LOCUS       DAOOLJ010000001       103232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_68_length_103232_cov_16.549347, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000001 DAOOLJ010000000
VERSION     DAOOLJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 103232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 103232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103232
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68_length_103232_cov_16.549347"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..2320
                     /gene="betT"
                     /locus_tag="PLW16_00005"
     CDS             332..2320
                     /gene="betT"
                     /locus_tag="PLW16_00005"
                     /inference="COORDINATES: protein motif:HMM:NF007399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HPB69315.1"
                     /translation="MNQAKADSDTKPRMKINPPVFFGAAGLIVLFVVFTIIWPERTGA
                     IFGSVQNWIVHSAGWFYVLAVATFLIFVVFLAISDFGRIKLGPDHSEPDYSYGSWFAM
                     LFSAGMGIGLMFFGVAEPVMHYMKPPVGDPETVQAAREAMKITFFHWGVHAWGIYAVV
                     AISLAYFAYRHNLPLTIRSAFYPLIGDRINGPVGHAVDIFAILGTMFGVATSLGFGAM
                     QVNAGITYLIGAPNNVYVQILLIAVITGIATISVVLGLDGGIRRLSQLNMFLAAGLLI
                     FVLIVGPTVYLLQTLTQNIGAYASDLVNKTFNLYAYQPSGWIGGWTLFYWGWWIAWAP
                     FVGMFIARVSRGRTIREFVTGVLLLPAGFTFMWMTFFGNTALHMIMVQGATDLGNAVA
                     ADTTMALFNFFEHLPFSAILSFLATILIVTFFVTSSDSGSLVIDMLASGGEDDPPVWQ
                     RIFWATSEGLVAVALLLAGGLGALQTAAIASALPFTIIMLLMCWGLVRALRVDVAKRI
                     SRRSAVLMPQTAGKPVSWQRRLQNVLRHPVESEVIDFINTTVRPALEDVAEEFRLKKM
                     DVEVKPGEDGRMWLEVLHGEEHNFYYGVRPRTYTPPAFAMRDTQKRRGEALKFYRAEV
                     YLSEGGQDYDLMGWSKEQIITDVIDHYEKHMHFLHLMR"
     gene            2498..4366
                     /locus_tag="PLW16_00010"
     CDS             2498..4366
                     /locus_tag="PLW16_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HPB69316.1"
                     /translation="MEQTPEVGRRRTFYDEAPDYRRPLLEIPADRKARMRERLSCLYG
                     DTAAGEWLPELERIIKVHFAHKSPEMIEKEKAYDPRERFSNDHLILITYGDSVRGDGP
                     TPLASLRRFIDTFTRGAINTLHILPFFPYSSDRGFAVIDFRRVDDKLGSWADIRKTKQ
                     RYDLMFDAVLNHCSSRSRMFREFLNGNPVYRDFFIAYDSPDDLTPEQRGLIFRPRTSD
                     ILTRFMTIDGPKYLWTTFSADQIDMNFRNPAVLMAVIDSILFYVRRGADLLRLDAVTY
                     LWSEPGTESAHLPQTHEIIKLLRDVVDVAASGVALVTETNVSHRENVAYFGDGRDEAH
                     LVYNFALPPLVLLAFYRGDATPLSQWAQGLEPPSDRTAFLNILDTHDGIGLMGVRGIL
                     PEEEIGFIVETAREMGALVSGRNNREATEEPYELNTTWWSALNRSDAGESLDFQVRRF
                     LASRSIALTLKGVPGIYIHGALGTPSDLDAAEASGEKRDVNRAIVDTRTLMADLWDPG
                     SKLSLLLRYGTHPIQVRRRERAFHPRGAQRVLLLSPRVFALWRTSPEGEESVLAITNV
                     TATTAAVEIPVASLGMAGKAWKSLLSGREWLPEGDTWTLALKPYGVVWLKPVEPSP"
     gene            4547..4657
                     /locus_tag="PLW16_00015"
                     /pseudo
     CDS             4547..4657
                     /locus_tag="PLW16_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4736..5083)
                     /locus_tag="PLW16_00020"
     CDS             complement(4736..5083)
                     /locus_tag="PLW16_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HPB69317.1"
                     /translation="MKRFIIAGILCLALVSVAHGQILKDVTSAARDKASTVAKDAANA
                     VVDDSSITAEVKANLFADTSLKGSKIKVQTKEGVVSLSGTVKTPQAKETADTIAKAVT
                     GVKSVKNKIAVKK"
     gene            complement(5198..6094)
                     /locus_tag="PLW16_00025"
     CDS             complement(5198..6094)
                     /locus_tag="PLW16_00025"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB69318.1"
                     /translation="MAGLREMLGCEYPIMQGPIGALNSPRLVAAISEAGAFGMLALGF
                     SGPEEAKKLAGEVRGLTDKPFGANLMIMNPSNAEILEILKDAGVTMVTTSVGAPSKIY
                     PLIHALGMHGLHVVLSLPHAVKASRDGVDGLVVSGAESGGIRSVASESSTMVLIPLVA
                     DHVAVPIVAAGGIADARGYRAALALGAQGVQIGTRLIASEESPAPQAWKEAIVACRDG
                     GTSLLPVREMNTKMRVIVNKKMQDLLADPAADVSRAFPFANAPKAWTTGDFDLFPAGA
                     GQIAALIQEIKPVKAIVAEMVS"
     gene            6297..7085
                     /locus_tag="PLW16_00030"
     CDS             6297..7085
                     /locus_tag="PLW16_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69319.1"
                     /translation="MLSKDYVEARELSEGIARAVGSPRFYIERHAELERSQALFDGAP
                     LVEKALRLAAGYGDCLGHGQSHVRKVAIDTGALVLIEGAGDPDLPRLVLLAHLAGLLH
                     DIKRSEKDHAHRGAEEAALILKTFPLRMAENRAITQAIGNHEAFRTYETLADPRAELL
                     SNALYDADKFRWGPDNFTETVWAMVAPRRIPLRVLMAHFLPGLEGVRRIRETFRTPTG
                     RRYGPDFIDRGLEIGRRLHEALMQKIDAEGVEASRPPRGTPEKG"
     gene            7092..7490
                     /locus_tag="PLW16_00035"
     CDS             7092..7490
                     /locus_tag="PLW16_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB69320.1"
                     /translation="MHSLTLRTASRFEMTDITDRINGMIGENGIVSGIAVMFVPHTTA
                     AVTINENADPDVPRDILASLDRAIPLKDRYLHDEGNSAAHVKASLVGSSEIVLIEGGR
                     LALGTWQSVFFCEFDGPRTRQIWIKLAGTS"
     gene            complement(7597..8100)
                     /locus_tag="PLW16_00040"
     CDS             complement(7597..8100)
                     /locus_tag="PLW16_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB69321.1"
                     /translation="MPIRNLVPSLWRGSAPARREPEHPFDALQQEMNRLFDSFFRGFD
                     AAPVGAMEGRLMGFTPFVDVKEDEKEVVVKAELPGIDEKDLDVSLSEHALTIKGEKKE
                     EKEDQGKGYYRMERSYGSFHREIPLPASIDAKKVEAAFKNGLLTVTLPKTEEARKADR
                     KISVKSQ"
     gene            complement(8136..8540)
                     /locus_tag="PLW16_00045"
     CDS             complement(8136..8540)
                     /locus_tag="PLW16_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB69322.1"
                     /translation="MAEDKKDMVKQPAATPAGTERTRARRVFVPRVDIYETSEAIVLL
                     ADMPGVDETGVDITLEKNILTLVGQAANGARAGYSLAYAEYDAGDYERAFTISDEVDR
                     ERIEATVKNGVLRVVLHKAEAVKARKIAIKAA"
     gene            complement(8560..9000)
                     /locus_tag="PLW16_00050"
     CDS             complement(8560..9000)
                     /locus_tag="PLW16_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB69323.1"
                     /translation="MYGPVLWRFGRAMDPWPEIQRLQQEMNRIFSSASQPYGQEFPAI
                     NAWAGEEGMVVTAELPGADPEKLDISVVGDSLTISGNREAEALKAGESYHRQERSCGR
                     FTRTIQLPFHVDAAKVAATYERGILNVVLPRSEAEKPKKISIKS"
     gene            complement(9224..9997)
                     /locus_tag="PLW16_00055"
     CDS             complement(9224..9997)
                     /locus_tag="PLW16_00055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB69324.1"
                     /translation="MMDMLNFAVTIAREAGSLLKGAFATPHRITYKGDINIVTEADLL
                     AENHLRERIRARFPDHDILAEESADAGTGAEYRWVVDPLDGTTNYAHGYPVFCVSVAL
                     ECRRQIVLGVVHNPMLDECFSALRGGGAFLDGRALSVSRTDALTRSLLATGFPYDIRE
                     SRDNNLDHFETLALKAQAIRRAGSAALDLAYVAAGRFDGFWELKLAPWDTAAGWLLVE
                     EAGGRVTDLDGGPYHLHAQHILASNGRIHPDLLELLSQT"
     gene            10176..11060
                     /locus_tag="PLW16_00060"
     CDS             10176..11060
                     /locus_tag="PLW16_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB69325.1"
                     /translation="MEEADDKSGSGILDLKALRESRGLTLENVHQRTRISMTNLEALE
                     AFRFEALPIPIYTRGFLRLYAQAVGIDPAPLLEHHRQYLNAISTTAPTEVPAGRPLKR
                     LPGKTVKRLLWSAALIVLVGVLFIALTAEQRTPGLAGKTVERSADLSPDAGSDAPAPG
                     MQKDPQEAGMINPVMPENRVSTPTAPADPKPPAAAVTGQAVPPDDPARIVKGQAPPYR
                     LALEARELTWIRITQDQEQPYQILLNPGDRVERVALQGFTLDIGNASGVAVVFQGRSL
                     GELGDPGEVVHLRLPAAP"
     gene            11226..11429
                     /locus_tag="PLW16_00065"
     CDS             11226..11429
                     /locus_tag="PLW16_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPB69326.1"
                     /translation="MFGIGMPELLIILVIILIIFGAGKLPEIGGAIGKGIKNFKKASR
                     EPEEIDVTPDAKKIDEKKPSANP"
     gene            complement(11426..11839)
                     /locus_tag="PLW16_00070"
     CDS             complement(11426..11839)
                     /locus_tag="PLW16_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPB69327.1"
                     /translation="MVNKVILVGRLGADPEVRYTPDGMNITNFRLATDEQRKDKNGDR
                     VKVTEWHRIVTFGKLAEICGNYLAKGKMVYIEGRIRTNAWEDKEGVKRYTTEIIASDM
                     RMLDSKGTGKGPESGEEAPAPASAFGPAPDEDVPF"
     gene            12034..13518
                     /gene="lysS"
                     /locus_tag="PLW16_00075"
     CDS             12034..13518
                     /gene="lysS"
                     /locus_tag="PLW16_00075"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPB69328.1"
                     /translation="MEDSELLRKRREKIDALKADGVDLYPNDVRVDHTTGEIQDRFGH
                     LDEAALQDVPETVSLAGRIMAIRNFGKAAFAVLQDRKGRLQVYIARNILGDAPYTLFK
                     RLDIGDIVFVAGRVFRTRTGELTVEAHALRLIAKSLHPLPEKWHGLTDVEIRYRQRHL
                     DLIVNPKVRDVFYRRSRIIQLIRRFMEDRDFLEVETPMMQPKAGGAVARPFKTFHNAL
                     GMDFYLRIAPELYLKRLITGGLERVFEINRNFRNEGMSSFHNPEFTMMEFYQAYATFL
                     DLMTLTEDLFTHIARDLFGGLTFTYQGTDIDLTPPWTRLTVQEAILQYTELPPEVLRD
                     PARAVAQARARGLDVKDDDPLGKVIMAIFEETVETRLVQPHFITHYPVEVSPLSRRSV
                     DNPGLTDRFELYICGREIANAFSELNDPADQRERFLMQAKDRDAGDDEAHVMDEDYVR
                     VLEYGMPPTAGEGIGIDRLVMLFTDATSIRDVILFPHMRIKEDA"
     gene            13520..14797
                     /locus_tag="PLW16_00080"
     CDS             13520..14797
                     /locus_tag="PLW16_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HPB69329.1"
                     /translation="MSYELFISRRYLRARRKQVFVSIITLLSIAGILLGVAALIIVLA
                     VMNGFETDLRDKILGINSHIVLMEYSGVMRDPDRVLERVRRTEGVAAATPFIYAQAML
                     KSGPRVSGVVLRGMDTATASQVISLGRMREGKLAYLDAPPPPASGLSGDLRGLPGIVV
                     GKELAKNLGLFLYGPLYVISPAGTATPMGMAPRMKKFVVVGIFDSGFYEYDSTLAYVS
                     LRDSQELLQMGGSVTGIEIKIRDLYRADAAAKRMEQALGYPYWARHWMEMNKNLFSAL
                     KLEKRVMFIILSLIVLVAAFNIITTLIMVVMEKTKDIAVLKSMGATSRSIMRIFVYQG
                     LIIGAVGTLLGCATGLAVAFNLEKISAAIETLFGFKILPGDVYYLSTLPSQVNYGDVA
                     VIVAGSLLISLLSTLYPSYRAGRLDPAETLRYE"
     gene            14801..15475
                     /locus_tag="PLW16_00085"
     CDS             14801..15475
                     /locus_tag="PLW16_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69330.1"
                     /translation="MAIAIQNLWKTYIKDGNRIDALKGIDLDIAAAESLALVGVSGAG
                     KSTLIHILGTLDRPTQGRVLYNGVDVFTWPEKRLAAFRNTRIGFVFQFHQLLPEFSGL
                     ENVMLPALIGGLAKREARKKAEGLLKDVGLGDRMTHKPGELSGGEQQRVAVARALIMA
                     PDILLADEPTGNLDTETGKIIEDLLIDLHRERNVTLVVVTHNQSLAQRMSRSVGLRDG
                     RIDPRA"
     gene            15468..18146
                     /gene="bamA"
                     /locus_tag="PLW16_00090"
     CDS             15468..18146
                     /gene="bamA"
                     /locus_tag="PLW16_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPB69331.1"
                     /translation="MPEKPHPLAPACRLLLLLMALLCLWSAETSAEEGVKSGPKKAAL
                     LPFAVHSRSDAAGLQTAIGKALDAELRKSSRYLELIPGSAFAPEIEGKPLDDALALRV
                     GQTTGADVVLRGSLTEFGESVSVDVSLLFVRSGKSVSGISAQGKGREDLPRMASQLAA
                     DILIRTSAAARLAKIDIQGNRRIESDAIRQAVKSTAGQLLSDETLSRDLKAIYRMGYF
                     EDVQASVADGPEGKVLTFTVRERPMITEVRIKGNKAIDTGDIEAVLGVKTRQIVNREK
                     VKEDVEKIRALYIGKGYYNVEIADAVEGDGDKEVRVVFTITENKRLFIKAIRFEGNQA
                     FTEKQLRNMMATNEWGLLHFFTDSGVLKTDVLKQDIGKLNAFYLNNGYINAQIGEPEV
                     THDAKGIYVKIRVVEGKPFKVGNVEIHGDPLQTPREALREKLAILGKTYYNREAIVRD
                     IDTLTRVCNDEGYAYADVTPRTKAQEKTQTVDIVYEIAKGKQVYFNRISIVGNTRTRD
                     KVLRRQLNIVEGDLYNSSKMKQSYMALNRLRYFEEVDFQSERGPDDTLTNVIIRVKEK
                     PTGMFSIGAGYSAQDAAMVMAQVSQQNLFGRGQILSLKANIGSETAMYELSFTEPWLF
                     DIPLWSQFSLWNFTREYDTYDLDSSGVGATLGYPLWERITGYLGYRLSIDDVYNIDEA
                     DASYYVKEQAGETTTSSASLTLSRDTTDDTLFPSSGTRISASVTYAGGPLGGDHDFTK
                     YGATAAWFYPLPLDMVFNIRGRIGYLQRNGDTKLPIYERYVLGGISTLRGLRDVGPKD
                     ERTGDIIGGTTMLCGSVEIIFPLIRNAGMKGVVFYDTGNTWESGYSLSDLRQTAGVGI
                     RWYSPIGPLRLEWGYVLDKKDDEDASRFEFTIGMFM"
     gene            18309..18848
                     /locus_tag="PLW16_00095"
     CDS             18309..18848
                     /locus_tag="PLW16_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPB69332.1"
                     /translation="MKRFLLLSLGGMLCLALLICIPEVQAAEKTGFIDMREVMLNSEA
                     GKKATEEFKKIYERDKASIQEKETELRKLKDDLEKQRSILKEEAYREKEAAYQKKFRD
                     YQLLVKDANEDLQARDQELSRTLIPEILKVVGAIGEKESYTLVLDIGTVPVAYFNKEN
                     DLTKRVIEAFNKTYKPKTP"
     gene            18871..19908
                     /gene="lpxD"
                     /locus_tag="PLW16_00100"
     CDS             18871..19908
                     /gene="lpxD"
                     /locus_tag="PLW16_00100"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPB69333.1"
                     /translation="MPTLKEIAAFLDGRVIGDPEVVIERVCGIDEAGHGDLTFVANPK
                     YRSRLATTGASAVLVASGTEAAGKNLVWVADPYVAFGQALGLLHPEDKAAAGIHPRAL
                     VEGEAFVSPEATVYPGVYVGQRARIDRGAVLFPGVFIGADAVVGEDSVLHPGVTVYRR
                     CLIGRRVTLHAGVVVGGDGFGFARPGRENRKIPQVGIVQIDDDVEIGANTTIDRGTLG
                     RTWIQRGVKIDNLVQIAHNVVVGENSIIVSQAGISGSTTLGKSVILGGQAGVVGHIRV
                     GDHVMAAARTGIHKDVPPSQIISGAPHRPHREWLRQEACLSKLPELRQTLKTLQRRVA
                     ALERQTAKKSL"
     gene            19920..20693
                     /gene="lpxA"
                     /locus_tag="PLW16_00105"
     CDS             19920..20693
                     /gene="lpxA"
                     /locus_tag="PLW16_00105"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPB69334.1"
                     /translation="MKIHPTAVISPEARLAESVEVGPYTIVGPDVEIGASTVIGPHVV
                     IEKCTDIGEGCHIRQFASIGADPQDLKYQGEPTRVVIGNFNTIRECATIHRATTADIG
                     VTMIGDHNLLMAYTHVAHNCKIGNHVIMSNAVNLAGHIHVEDFAIIGGMTGIHQFTRI
                     GAHSFIGGASAVPRDVPPYVLVSGNTAKPYGLNLVGLKRRGFSEDTLQALKHAYRLVF
                     RSSLRLAVALDRVEQEVPDLPEIRQFVEFIRHSARGICR"
     gene            20703..21650
                     /locus_tag="PLW16_00110"
     CDS             20703..21650
                     /locus_tag="PLW16_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB69335.1"
                     /translation="MAKIRIGVVGIGHLGTYHLQKFRMMDACVITAVSDSDGEKAAKA
                     AAEYGCAAFEDYRDLIPHVDAVSIAVPTAAHPEVAGAFLAAGIDVFLEKPLAPTLAEA
                     QSLVALAEANGRTFQAGFIERFNPAIVALKALLGVPLFVESHRLHPFFERGTDVDVIL
                     DLMIHDLDILAWFVPAPLAHVEAVGVRVLSENVDIANARLTFENGCVANVTASRVTGK
                     TLQKMRFFGLEGYHAVDFAQRELVSLKRVRQADGKIDIVDNRADVIPKDPLEAEIRAF
                     VEAVLRRTPPPVTGRDALAAQEMAGLIGERIQAVEARWA"
     gene            21733..22872
                     /gene="lpxB"
                     /locus_tag="PLW16_00115"
     CDS             21733..22872
                     /gene="lpxB"
                     /locus_tag="PLW16_00115"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPB69336.1"
                     /translation="MSGRRVMIVAGEASGDLHGARLVQAMRALDPALAFSGIGGRNLR
                     QAGVRCLADVADMGVVGLTEVTRKLGTILRARRDLRRILSDAPPDLLILIDYPDFNLP
                     LARIAKARGVPVLYYISPQIWAWRKGRLNAIRKCVDHMAVILPFEKPLYDQAGVPATF
                     VGHPLLDALGDTPAPPEARRMFQLAEGVTTVALLPGSREGEARKLLPEMLGAARIIAR
                     ELPPVQFVLPLADSLDPAVVEGIVRRRDLAVRIVRNRIYDVLPAADVALVASGTATLE
                     TALMNLPMVVVYKVSPLSYWVGRAFIHVGHISLVNIIAGKTVVPELIQGEAHANRMAV
                     EVIDILSRKSRKLRMHEELSQIRARLGEPGAAAKTAALAMDLLNT"
     gene            22923..24755
                     /gene="msbA"
                     /locus_tag="PLW16_00120"
     CDS             22923..24755
                     /gene="msbA"
                     /locus_tag="PLW16_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HPB69337.1"
                     /translation="MKPQPTPPRPPGKESGGDIYIRLLKLVRPHAPKFVLAMLCMLVV
                     GALTSALAFLVKPAMDDIFLKKDTRMLMLIPVAVVLIYLIKGACHYAQSIIMNFIGQR
                     VIADLRYRLYEKIQRQSLAFFTQNPTGVLISRITNDVNFMKEAVSEAVTNLFKDSFTI
                     VCLIFVIFYRDWKLAIIAMVVFPLTIYPIATFGRKMRKVATRTQVTMGSLTSLLQETI
                     SGTRIVKAFSMEEHENERFAGENERLFRLTMKSVAISAISSPFMEFLGGIGIAVIVFY
                     GGYQVIQGHSTPGTFFSFLTALIMLYEPIKRLTNVNNKIQQGISAAIRVFGIIDTTPD
                     ILDAPDAVELPRITQGIALEDVSFRYEDTPVLKNIALRIPAGEVLAFVGMSGGGKTTL
                     VNLIPRFYDVTSGRILIDGRDIRTATIASLRKQIGMVTQQTILFNDTVRNNIAYGSFE
                     KPEAEIIRAARAANAHDFITRLPQGYDTRIGEQGTKLSGGERQRISIARAILKDAPIL
                     ILDEATSSLDTEAEIEVQGALENLMQGRTTLVIAHRLSTVRNADRIVVLVDGRIIEEG
                     THDELLARQGEYAKLYAMQFKEENGNGVRNRTPSPSGSAATGEA"
     gene            24755..25834
                     /gene="lpxK"
                     /locus_tag="PLW16_00125"
     CDS             24755..25834
                     /gene="lpxK"
                     /locus_tag="PLW16_00125"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPB69338.1"
                     /translation="MHPQAALEGIWYRPGAQARRHPLFWPLYALSLPYGAAIGLRNRL
                     YDGGGLPRTKLPCPVISVGNLTVGGTGKTPLVMLLAELMQEAGWRPAILSRGFGGRSS
                     RGVHVVSDGRRLLATPETVGDEPVLMARTLPSVPVLTGPDRVRTGKAALDRFGAKLLI
                     LDDAFQHRRLARDLDIVLTDLTLPFGNGCLLPAGPLREPVQALKRARIVIRTGSEAEI
                     RTSPASADAGGLRGFHRPRELIRGDGAERHPPAMLAGKRLLAFAGLGRPDAFRKTLAA
                     LGAEVADFLPFPDHHAYRRSDIEGIRQRAVAAKVEFIVTTEKDAVRLAPCADFLGEIF
                     QLRIVMDILPERETLAARVLERIAP"
     gene            25831..26448
                     /gene="gmhB"
                     /locus_tag="PLW16_00130"
     CDS             25831..26448
                     /gene="gmhB"
                     /locus_tag="PLW16_00130"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HPB69339.1"
                     /translation="MRDAGHAAVFLDRDGTINEEVGYLDRIDRLRLLPGAAEGIRLLN
                     ASGFKVVVVTNQSGVARGLFTEAFVVEVHRTLAELLGRQGARIDRFEYCPHHPTEGAG
                     RYRRACPCRKPEPGMLLKAAAALAIDLDRSYVVGDTPRDLEAARRAGARGILVRTGYG
                     TEHLKAASAAETTAAHVADDLLAAVHWILTEADRHGPDPRDRRRP"
     gene            26445..27509
                     /locus_tag="PLW16_00135"
     CDS             26445..27509
                     /locus_tag="PLW16_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPB69340.1"
                     /translation="MNLLIVKLSAIGDVIHTLPSLAALRRHFPAAHITWVVEEAASDL
                     LADHPHLDRVLVSRRRRWQSDLKRGRVRQPLRAIAAFLGDLRSRPYDLVIDFHGLFKS
                     ALLVGLSGGRRKLGYDSLQEGSGLFYTEKIPEDLTRHAVDRYLDFARHLGIEPGKAEF
                     LIPDTADQRGRIDSLLQGLGIAPRERFIALSPVAFWETKLWEEARFSRLGDRIRRELG
                     LPVVLTDGACDYTARIARGMTEAAVNLGGRTTLRELAALYRRAALLVTTDSGPMHLAA
                     AVGCPVVALFGPTDPRRTGPYGPGHTVLESPVDCRPCFRKTCETRQCMRDITVDAVFE
                     AVREKLTDRRQKMHAEKTLE"
     gene            27520..27699
                     /locus_tag="PLW16_00140"
     CDS             27520..27699
                     /locus_tag="PLW16_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPB69341.1"
                     /translation="MTLNKDLLDILACPKCKGEIHLNDAGDGLICDACRLRYEIRDDI
                     PIMLIEEARSLDEGP"
     gene            27696..28586
                     /locus_tag="PLW16_00145"
     CDS             27696..28586
                     /locus_tag="PLW16_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB69342.1"
                     /translation="MIYAKLFLTALFWGGTFVAARVVTQEFSPFSASLWRFVIASAVL
                     AVPVVLHEGGLPRLRRRRVLPVVLLGLTGVLAYNVFFFLGLKTIPAGRAALIVANNPV
                     FIALFSALLFKERLSIANGIGIGVCLAGAVIVISRGNPGGLLSGAIGPGELFILGCVA
                     SWVSYSLIGKVVLRELSPLAAVAWSCLIGAAALLPFAWQEGLVRQTALASPAAWAGLL
                     FLGLPGTAIGFLWYYEGIRAIGPSRAAVFINFVPVSAVLFGWLILSETLTRSLLTGGA
                     LVVVGVFLTNRAGGRKTLET"
     gene            28702..29487
                     /gene="xth"
                     /locus_tag="PLW16_00150"
     CDS             28702..29487
                     /gene="xth"
                     /locus_tag="PLW16_00150"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPB69343.1"
                     /translation="MNPFTIATYNVNSIRSRLHILLPWLETYRPDVFCMQETKVDDGR
                     FPADAFTNLGYHCIFRGAKQYNGVAIASLAAPEAVRYGFSDGGSPDDDRLVCGVFDGV
                     AVVNAYVPQGRDRESEHFAYKLAWFKRLKTFLAAHYSPAQPLVCCGDLNCAREAIDVH
                     DPKRLLGHVCFTPEVWDAFDALTAWGLEDLLRRHHPGEANHYTFFDYRVPRSLERGLG
                     WRIDHLLVTAPLAERSLGCRIDLEPRRAVKPSDHTILIGEFRR"
     gene            29501..30043
                     /locus_tag="PLW16_00155"
     CDS             29501..30043
                     /locus_tag="PLW16_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69344.1"
                     /translation="MDEIQETLRWNEDVARKLSGIEREIADSPDPAGLFEHLVLSMET
                     AFGIPFVWLSLVRTPQADALRGTLAASGLLGDRLNLIDGDAFAALLDGRSEPLLVNGD
                     LRSFYRLLPPMKKFLFRSLALVPFTLDGTVVGSLNHGDPSPQRYHPDLATDLLAQLGA
                     RVSARLGALSSKAVPLPASP"
     gene            30217..30498
                     /locus_tag="PLW16_00160"
     CDS             30217..30498
                     /locus_tag="PLW16_00160"
                     /inference="COORDINATES: protein motif:HMM:NF040962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HPB69345.1"
                     /translation="MVKADPKASCCAPGNRTPSGCHIESLVSVDERGQMVLPKDLRDK
                     AGIKAGDKLALVSLKKDGEICCLALIKVDTLADQVKEFLGPVMRDAVTG"
     gene            30583..31686
                     /gene="hgcA"
                     /locus_tag="PLW16_00165"
     CDS             30583..31686
                     /gene="hgcA"
                     /locus_tag="PLW16_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264175424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HPB69346.1"
                     /translation="MASPQALSAADIALPMRPPGLDQPFVNGAAEGPAGKIPRVSSVL
                     TWRDSWGSIKARWGVGRMDYAVDPGLYALGSPDAGSPVLVTANYKMSFDRLREALTGR
                     NAWILVLNTEGINVWCAAGKGTFGTGELVRCIVASGLKDCVRHRELILPQLGAPGVSA
                     HWVRKMSGFTVHYGPIRARDIPAYLDGGLRATPKMRRKTFSLPERAVLIPIELVAALK
                     PVALLVPILVLISGLGGRGAFVQNLIHDGLFSLTALLLAILSGAVLSPLLLPWLPGRA
                     FATKGLAVGLAAAIALLSSWGLNLKSGRDLLLGGAWLLMMPGISAFLAMNFTGASTYT
                     SLSGVRREMRWALPVEIGAGLSGLAVLLVSRFF"
     gene            31705..32019
                     /gene="hgcB"
                     /locus_tag="PLW16_00170"
     CDS             31705..32019
                     /gene="hgcB"
                     /locus_tag="PLW16_00170"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HPB69347.1"
                     /translation="MIYLKNVVTLEVDRDKCTGCGMCLEVCPHAVLAMEDRRIRIRNR
                     DACMECGACARNCPVGAVSVEAGVGCTAAVINGLLNRQAPSCGYSLDPKRPSAGKVGG
                     CC"
     gene            complement(32070..33875)
                     /locus_tag="PLW16_00175"
     CDS             complement(32070..33875)
                     /locus_tag="PLW16_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB69348.1"
                     /translation="MKKLEETSMAAVFQNRAREHGDKACVAYRNDAGQWFDVSWNRMN
                     EMVHNLAYYLMGKGIQHGDKVAVFSPNRYEWWVADLAILSIGAVNIPIYATNTAEEVR
                     YILDNADSRICFAGTHAHMEKVVAVRDRLPMLMEIVLFNDLEQPVDGVVTLPEAIRQG
                     GAQADAALFDRRLAAIKPEDLCTFIYTSGTTGDPKGVMLSHHNIISNVHQFNAYFPEM
                     VNRFHKAPSFLPLSHAVERTAVYYCQVYCANKIHFVKSIETLLEDLQTIHPSIMASVP
                     RVLEKMYEAITAKVAQAPPLKQKLFAWALDVGRRNVPFVCKGKARTGLFAIEYALANK
                     LIFSKLKSVLGLDQIYFIGLGGAPMPRKVLEFFLGMDIPCCDAYGLSETSPMVTCNYV
                     GHIQPGTIGVPVIDTEVKISEEGEILVKGPQVMVGYYKNEAATKGAFTPDGFLKTGDL
                     GEFDAEGNLRITGRTKELIITSGGKNISPQNIENSLMFSPYINQLSVVGEGKKFISAL
                     IVPAFEVLEPWAKENGVTFADRSELVKHAKVQALFEQQIKAFTKDFGQVEQVKKFTLL
                     DREWLQETGELTPTLKVKRRVIAVKYADAIEKMYS"
     gene            complement(33945..34517)
                     /locus_tag="PLW16_00180"
     CDS             complement(33945..34517)
                     /locus_tag="PLW16_00180"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPB69349.1"
                     /translation="MNADAIEDALKMNEDLAPYCRKALENGAAHFRITHPGMVATAPW
                     VRWKCQFGCPGYGMGYCCPPHTPTDDQTRALLDSYRRAILFHIEAPATPERGKRYRAF
                     MDMLVSLEGELFKDGYYKAFVFLAGPCILCKKCGKLSNVSCLHADRARPAMEACGIDV
                     FQTARNNGFFIETLRVREQTNNEYCLMMVD"
     gene            complement(34537..35418)
                     /locus_tag="PLW16_00185"
     CDS             complement(34537..35418)
                     /locus_tag="PLW16_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB69350.1"
                     /translation="MVNFTQYMISGLSAGSLYALVALGVVLIYRSTRILNFAHGDMAT
                     MGTFFAFSLLSWRFPFYLALPLSLLFGALLAVVFYFSIIVPAQRRDATLLGQIALTVG
                     LSMIFQGAMIYVWGTDSKMLAFPLSDTKTWNVGPIYLNELSLGTLIVGILGSILLYLL
                     IQKTRLGLAMRATSENLPAAQTLGIPTRTILATSWGLAALLGVLAGLFLAPALILDPF
                     FMLEPFLKGFAGAILGGLNSLPGAIIGGLLIGIIENLCGGYISLEFKNTVPFLIIILV
                     LLFKHEGLLGKEFKERI"
     gene            complement(35438..36418)
                     /locus_tag="PLW16_00190"
     CDS             complement(35438..36418)
                     /locus_tag="PLW16_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB69351.1"
                     /translation="MNLVESYRDDLKLIEKPWTGCWIGMTLLVLVLLPWFSSAHWVYL
                     GTLIFIVAVGIMGQNLLIGYTGQISLGQAGFLAIGAFTFGHLAKLGIPVGLALLAAGL
                     MAAFFGVLVGFPSLRLKGPYLAIVTLGFGIAVYQTFTSFEVLSGGRMGLVVPKFPALF
                     GFSREVSMYYFALLLGLTFTLVTYNLVSSYLGRAFVAIRDNDIAAEVLGVNLTNYKLL
                     SFAISSFYTGIHGAMFGMFMGFIEPQMFNFSESLIIFVAVVIGGLASVEGAIFGAAFV
                     ILIPQIFSGYREFVPVIFGVTVVLIMIFEPLGLAGRWFKIRLYYRNWPFR"
     gene            complement(36451..38262)
                     /locus_tag="PLW16_00195"
     CDS             complement(36451..38262)
                     /locus_tag="PLW16_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69352.1"
                     /translation="MPLNAMAVPQAFFEQVEKHRTRVALRHKQYGIWNKVTWGQYGEN
                     VRQVAAAILSLELPRGQTVCILGDNRPEWLICHMAIMTAGMRTCGIYPTSSPDEIRYI
                     IDHSEAQLLFAENEEQLDKILDILPNLRLQRIVVWDEKGLWGYTHPRVVFLREFIRRA
                     EAHLADHPVAVTDSLRAIDVEQTAMVIYTSGTTGRPKGALLSHRNISMVTQAFLQIIP
                     VSEKDEVVSYLPLAHIYENLVSVFMPVFTGVTVNFVESLETLPANLREISPTIFGSVP
                     RIWEKFASNIHIKIDDSTPLKRFLYRRCVQVGRRYQQARQAGAGSLLVWRAVYALAYW
                     GVLYHLKRQLGFERIRWALCSAAAASKELFEFFNILGIPLRDGYGQTESTGIIALQRL
                     DKEPRYGYVGEALPGLEMRISTEGEILARGPGVFQGYFKNPELTEETLRDGWLHTGDL
                     GAIDDGWLKIVGRMKDILITSGGKNITPEFIENKLKFSTYIQDAVIIGDGRHYLTALI
                     LIDEDNVVKFAQDNRIPFATFEDLTQNPAIVKLLDGEVKEVNETLARVETIKKFALIP
                     RRFYAEDGDVTPTQKVKRRNVEKLYKDLIDEMYRGEA"
     gene            complement(38262..39035)
                     /locus_tag="PLW16_00200"
     CDS             complement(38262..39035)
                     /locus_tag="PLW16_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69353.1"
                     /translation="MSFLNIASIETLYFDRIYALRGISLTVRENEIFTVLGPNGAGKT
                     TLLKTIAGLLKDQPKKGTIEFCGRRISGLTPEKIASLGIVYVPEDRGLFRELTVEENL
                     DLGCWGRRDKAVRNDLNFIYDLFPILKERGGQQAETLSGGEQQMLAIGRAILRRPKLL
                     MLDEPSLGLSPIMARSVYDTLRKVSERDMTILLVEQNARMALDIADYGYIMEAGRIVL
                     KGTSQELKENDDVKELYLGLEQGNASEKGWKFYTKRRRW"
     gene            complement(39032..39787)
                     /locus_tag="PLW16_00205"
     CDS             complement(39032..39787)
                     /locus_tag="PLW16_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69354.1"
                     /translation="MSLLKVNNLTVQFGGIKALSAIDFEMEEKEILSIIGPNGAGKTT
                     LFNCVSGVYRPTGGEVLFAGESLIGLSPDRIAQRGIGRTFQNIRLFNNMSVLDNLMLG
                     RHMKFRRSLLHAFLRIRKEELIHREKVEEIIDFLDLAAYRESRVIDCPYGVQKRVELG
                     RAMVMEPKLLLLDEPVAGLTVEEKNRVAYLVTEMRGRFGFAVLLIEHDLRIVSRLADR
                     MVVLNFGEKLAEGPPAEVQRNPEVIRAYVGETS"
     gene            complement(39803..40984)
                     /locus_tag="PLW16_00210"
     CDS             complement(39803..40984)
                     /locus_tag="PLW16_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB69355.1"
                     /translation="MFWILLIIIGFSLPATVLAAGTPGVTKDEVTIGMTCPMSGPAAL
                     WSAMALGNSAWAKHINDQGGIHGRKIKFVVKDDGYNPSRALANLTEMKDDVLAYGCVI
                     GSATANASKDFLLTSKVPVVHVHANPRIWLGTPAAQRRNIFIAYPDYIDESEAVAKFA
                     VTKMGIKTIALFGQNDDWGKSAEIGIQKAIKALGGKGKFAGFVTFEVTDRAVGGHALK
                     LKELGADAVVIYGAPTQAALIIKEMAKIGYKPKIFTANPLGDPLMYKIAGELWEGAYP
                     AVSGNVSLPGVDPQADRVVDILLKYEPALKGREFLGVTGATTMMLIAEGLRKAGKNLT
                     RESFVKAMEGIKNFKCEGMGAPITFSAKQKMGLNAIRICEAKGGKHVPISEFMIFNPL
                     F"
     gene            complement(41109..42251)
                     /locus_tag="PLW16_00215"
     CDS             complement(41109..42251)
                     /locus_tag="PLW16_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69356.1"
                     /translation="MDFALTEEQGFIRDTIRKFVAKACDREQIKRMDEEGAFPAELFR
                     KVSDIGLCALTIPEADGGGGPNTLGAVIVTAELAGIYPPLAGTFATHSLCGGKVLADL
                     GTEAQRERYLPGVIDGSSLVTLALLEPDQGYDQSGTRTTARREGERVVLNGAKTFVRL
                     ADRAALILVRAALEDGDGTPAFFIVAAGTRGIRIRALETVGFHGMSLCKVGLDAVAVG
                     AEERLGGDADAPTERKKLAGVMSAEHLAVAACGMGIARGAYAYALQYARERVQFGRPI
                     IQFGAIREMLVEMALQARVAELLTCQAASLIDRGRECLPEIIQARLAAAEAARKTSLH
                     GVQIFGGYGYAMEYDAQRYLRDAMVLLSGGGTRQVLGQALGVLWEY"
     gene            complement(42337..43920)
                     /locus_tag="PLW16_00220"
     CDS             complement(42337..43920)
                     /locus_tag="PLW16_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69357.1"
                     /translation="MATLFHGITCADLYDRSVAAFRSRTAILFNDETYTYDELQKNAY
                     SLAHALDKLGLKKGDRVAFLMANCPEYIFCEYALGKLGLVRVPLAVLLSSKDHIYMMN
                     QSDCKALIYHERMAGRVQEMIPSLETVKQFICVAQDPATVTSGHAHLQSLIAANPPEA
                     PRVALECTDLVAIYYTGGTTGLPKGVMMDHNTWVGTFISEMLHLGLDWDEVFAFATPL
                     THAGGCLILPILLKRGTCEIQDHFDPKLFLERVEKVKVTTTFIVPTMIYVLLDYPDLK
                     KYDLASLRNIIYGASAIAPARLKQAVDTLGPIFSQLFGQTESPMMISALSRQEHVIAD
                     PERRMRIYTSCGRPVMTADVRIVDENGKELPRGEAGDIVCRTINMMTGYLKNPEQTAE
                     TIRDGWLWTGDVGKFDEEGYLYIVDRSKDMIISGGFNIFPREIEDVLHEHPAVKNVAV
                     VGVPHEKWGEEVTAVVSLHAGKTASEKELVQFVKERKGSLLAPKSVKFWDEIPLTNLG
                     KLDKKKIREMFWKGMDRKV"
     gene            complement(44004..46013)
                     /locus_tag="PLW16_00225"
     CDS             complement(44004..46013)
                     /locus_tag="PLW16_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB69358.1"
                     /translation="MGRIFNCSNEDGIAVVRFDVPGEAMNTWTEEAIADFAVLLETLE
                     RQKVAYRGVIFISGKSTNFHAGANLNMLGAEKDLADFRKGCDAFNGLFIRLENLGIPT
                     VAAIHGPCMGGGYEFALTMTARIATDGKRTTIGLPECNVGVIPGAGGTQRLPRLIGYP
                     ALDFIPRGAVLPAAKALEVGMVDRVIPEGEDLLAKAKALLAEILGGTAGLKREKPDLS
                     ALDATLEAAKKNVLKATRGRAIPAPMLALKAMSEGLKVPIEQGLKIETDCFIEALKAP
                     ECKGTINTFFLKTLSDKPQAMIPRGFEPKPIRKFGVLGFGTMGRGIVINILTRTRLPI
                     VVKDVSGALEPGMGFLRKILEGMAAKNRLKEPVDSLIARVKPVAEWTHDFKDVDCVIE
                     AVFEDPAVKDQVYRELCQAVADDCLLATNTSSLPVDKLAKSVSNPERFVGAHFFSPVW
                     MMQLLEIIRGQATSDATTNNMLAVCAALGKRPVVCNDNPGFVVNAMLFPYFIKAFDLL
                     AEGVAMDRIDAAMMKFGLPVGPIRLTDEVGIDVSYLVLTKSLGLVPPPALERVYKAGR
                     YGRNKNGKGFYAQDGAPDPEVLPLINPDNRQKEYSVAELQDMLFTPFVQTGHELLRKK
                     VVSDPRSIDIGAIWGVGFPADKGGPMKWGDLIGLSEKLYGKKFYT"
     gene            complement(46085..47242)
                     /locus_tag="PLW16_00230"
     CDS             complement(46085..47242)
                     /locus_tag="PLW16_00230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69359.1"
                     /translation="MDFNFSEKQNALREKVRGYTEANISRAVVREIEERDEFPHDLVK
                     KFCELGLARINIPEAYGGLNGDIVDLMIVFEEIAKRSPVLCWNLGNILLYGNEIIGVN
                     GNQAQKDEYLPKLARGEIMFSFAITEPNAGSDAASIKTRALEAVPKDGSYVINGGKMF
                     ITGAGVTQYTVTFARTAESKYGGITSFIVDTSLKGYSAKPIKKLGFHGSNTCEVVYDN
                     VRVPPQSILGGEEKGLNNGWKQEMKLLNQERLVLSSMTLGMAQSSFDEALQFAREKMQ
                     RTDPGTDVQSIQHDIAEMASELEAMKVLAYSAAWKEAQGLNPVRETSMTKYFCAETAK
                     QIILKGMNVLGESASSMDYEMQRNLREILIFSIGGGTSQIQKNIICKTFGL"
     gene            complement(47308..47757)
                     /locus_tag="PLW16_00235"
     CDS             complement(47308..47757)
                     /locus_tag="PLW16_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB69360.1"
                     /translation="MSQKYWEDFDIGFNFRTPSITVTETHVVNWAGLTMDFYPLHMDA
                     EYCAKTVFKERIAHGPLIFGMAVGMAGLAKVEGGSIIAWMGVDKMRMLAPVKFGDTIT
                     VHIEAVEKKETSKPSQGLQTWRYTVKNQRDEAVMVFDMKFLMRRKGA"
     gene            complement(47802..48221)
                     /locus_tag="PLW16_00240"
     CDS             complement(47802..48221)
                     /locus_tag="PLW16_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69361.1"
                     /translation="MDTPVKPKPIPVVNPWAKPFWEAARENKLIIQQCKDCGTYIFYP
                     RIACTNCFSDNLTWVPASGRGKVYTYTVVESYPPSAFIPDLPFVVAVVRLEEGVQMLS
                     NIVGCDPHSVYCEMPVAVVFEKLNEEFTLPKFKPVEA"
     gene            complement(48238..49422)
                     /locus_tag="PLW16_00245"
     CDS             complement(48238..49422)
                     /locus_tag="PLW16_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69362.1"
                     /translation="MSKLKGKVAIIGIGEVPTGRFPETAAIYHAIASARLAIRDAGIQ
                     KDEIDYVMPTAALFSPQFNTELVTCRVAEELGLKNVKKNCQVFAGGSSSTCALEIAAS
                     LIHSGAANMILFVHADRLGTGVSAQGGIDLFSTAGISAEWEVPFGQHYSAIAAMSTMR
                     YKYETGCTDEHISAVCVSNRKWAQLNPNAMFKKDLAIEEVMASKSLSTPLRVKHSNVL
                     ADGGAAFIVTSAERARAICERPVYLLGEGGVVTHFVFSQEPDVSRFGWAKAGRMAFEE
                     AGLTPKDVDVAEIYDSYPIYQLIGFEELGFAERGQGGEMFLKGDTWPGGKIPTTTNGG
                     MLSQGHTGAGGAVALMVETARQLMGKAGERQVQGARFAVETAVGGTYMDAQVTILGTE
                     IP"
     gene            complement(49442..50350)
                     /locus_tag="PLW16_00250"
     CDS             complement(49442..50350)
                     /locus_tag="PLW16_00250"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB69363.1"
                     /translation="MPPLIQTEFTAVMGIRYPIIGAPMFLVSYEELVAAVCEAGGLGM
                     LPLPNYRTTDQLRRALDIIRSRTEAPVGVNIHLSGKFPWQEQLDLCLEHGVKFFITSL
                     GDPRLILDAVHAHGGWVFADVVSLAQGLKAREGGVDGLVAVGAGAGGHGGRISTLVLV
                     PYLKETIGLPVVAAGGIASGAQMAAALAVGACAVIVGTRFIATPESRASAAYKQAVID
                     ADPDGIVCTDRITGNPANWIAASIADTEERPPLTSKKWLDFWSAGQSVAQVRDIKPAG
                     AIVAEMVREYGETCRRMQGTVVSNGT"
     gene            complement(50449..51657)
                     /locus_tag="PLW16_00255"
     CDS             complement(50449..51657)
                     /locus_tag="PLW16_00255"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HPB69364.1"
                     /translation="MATGIRDKVAIIGMGCTKFGERWDCGPEDLMVEAFQECLSDAGI
                     EKNDLQAAWFGSCLDEVNVGKSALPPSLALRLPNIGVTRVENYCASGTEAFRGAVYAV
                     ASGAYDICLAIGAEKLKDIGYAGLPEFSAGMGLTNQQWFPNMTAPGCFAMLASGYSAK
                     YGVDMKELKRAMAHVSAKSHANGALNPKAHLRAPVTEEVILKAPMIAYPLGLFDCCGV
                     SDGSACAIVATPEVARRLGKKDFVTVKALQMSLSNGEEMSFTNWDGSGFVTTAKASAA
                     AYREAGIVNPREEISMMEVHDCFSITELVTMEDLHISARGEATKDIMDGFYDLNGKVP
                     CQVDGGLKCFGHPIGASGLRMIYEMYLQFHGRAGQRQLENPRYGLTHNLGGFPFMNVC
                     SIAIVGRNEA"
     gene            complement(51660..53126)
                     /locus_tag="PLW16_00260"
     CDS             complement(51660..53126)
                     /locus_tag="PLW16_00260"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HPB69365.1"
                     /translation="MIGITSFGGYIPRLRLSRKLIAQNVAWYSPVIWGAAQGEKAVAN
                     WDEDALSMAVAAAQDCLVGRDRTQVDGVYAASTTFPFADRLNADILATALNLSEDGTA
                     CADFTGCLKAGTTAVISALEAIQAGGKKRVLVAAGDQRRTKMATNFEMFLGDGAAALL
                     LGSQEVIAEFKGGYSLSCDFIDHYRDAGKEFDYAWEERWVRDEGYGKIIPRAIFGFLK
                     KTGLTLGDFDKVVYPCYFSATHRGIAKKLGLDPAKVQSNLHEVCGDTGAAHPLVMFVA
                     ALEDAKPGDKILLVGFGQGCDVLYFQVTDAMAGYRGGQGVKGSLAKRAELTSYTKFMK
                     FRDIVTADLGIRGEANPNSSLNILWRNRKKLIGLVGSRCKKCGTPQYTFTPVCVNPDC
                     NAVGDMEDYAFSGQKGTVFMYTGDMLAASVDPPAIYGIVNIEGGGRLLADFTDCTLDE
                     IKVGLPVRMSFRRRSKDPVRGFSGYFWKVVPQATEKGV"
     gene            complement(53290..54732)
                     /locus_tag="PLW16_00265"
     CDS             complement(53290..54732)
                     /locus_tag="PLW16_00265"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPB69366.1"
                     /translation="MGLMTAQEYKDSLNDGRIVYYKGRKIDNVATDPDLKACVETLAV
                     DYEMAHDPRYKELALVYDEELGENISRYYHTPQNGQDLLKQLELIIKATELGDGYIPL
                     AHDIGADALNAIAITARAMKDAGKVYLERIENFRTYLKKTDKCIVAGVTDVKGDRSLR
                     PSDPKQNHPDFNVRIVERNDQGIIVRGAKVHITGAAYAHEFFVIPCRAMTDADADYAV
                     AFAIQANAKGIRQIVRPFHGRISDMEFPVKRELRRMHTDSLIIFDDVFVPWERVFLCG
                     EVKFAASIVYNFALMHRRTGCAYRIPLSEQLLGAAVAIADYNGVADAPHVREKITECV
                     IYLETLSALSRSACLDFVDHAGIPVPNPMTTNIAKYHFAHNYHKIVEIVQDLAGGLLV
                     TAPTYQDWCNPETHDDIDKYLGGKAGLPTEARLRMFDLIRRMTSADLETICLHGEGSP
                     YAERMTILMEARGLIKKCKQIVEELAAIKK"
     gene            complement(54791..55555)
                     /locus_tag="PLW16_00270"
     CDS             complement(54791..55555)
                     /locus_tag="PLW16_00270"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB69367.1"
                     /translation="MNTSYLSLQGKVAVVTGGSRGIGRAVALALADAGADVVVGSRKL
                     ADLEKVAEEIRAMGRKALAVAMHNREVESIKNFVDTVVKEFGRIDILVNNAATNPGMG
                     LLVDMDEKMYDQIMITNLKGYTILSQLVGKVMQQQKGGNIVNISSVGGVSPDVGLGLY
                     CISKAGIGMLTRAMAKELGDFGIRVNCIAPGVVRTKFSQALWSNEPMMKQELAHTPIK
                     RIAEPEEIGRIALFLASDASAYMTGQILIVDGGGSI"
     gene            complement(55860..57074)
                     /locus_tag="PLW16_00275"
     CDS             complement(55860..57074)
                     /locus_tag="PLW16_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB69368.1"
                     /translation="MKARMEWWCVVLAVALCLSLGSAWAADVRGVTDKEVKIACLVDL
                     SGPGKYGGPPIADAFRDYVAWVNDTGGINGRKINLIVEDNGILPTTTMNAAKKVILKD
                     EVLAIAMNLGSAGARAILPLCEENQVVLMPHGANKEFYSPGNKWVFVPHTVQFSMASR
                     AVEYILGKNRKARIGIIYQDDDFGKEGLEGARAAAKFMKTKLVAEAPYKTGTIDFTPH
                     MNAMKEANVDTIVLWTYLPQSAAIIKQKVKMGWDVQLISNNTTGVPPLFALTGEAADG
                     YMLVTPYAPGHMSLPGIKRIKEINAKYGNPAKTLGNPMYPDYLYLAGWGYIEAMGQGL
                     RKAGRNLNPETLVKGLESVKNFDMGGLSPNITFGPKQHVSSFSSLVLKADGKAKRFVI
                     IDSIKAPRTPQK"
     gene            complement(57222..58256)
                     /locus_tag="PLW16_00280"
     CDS             complement(57222..58256)
                     /locus_tag="PLW16_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB69369.1"
                     /translation="MANFKVSYQEDTALFQNLWVKSWLAIFVAAMIALPFLMSRYQLS
                     ILNEMGIAVIGALGLNLLTGYTGQISLGHGAFVAIGAYTTGLLTGALGLPFVIALPLS
                     GLMAALLGMIVGIPSLRLKGLYLALGTLAFGFIVEYVLFHWDLTKGDMGMAVPKIGAG
                     GFVIETERQYFFLIMSFAVLATLVAKNLARTKIGRCFVAIRDRDIAAEAMGISLAKYK
                     IMAFAVSAFYAGIAGCLMAHYQKWIVPGNFDISLSIAYIAMIVLGGMGTILGSILGAI
                     LITGIPHGIVFLTDAFRETYPSLSGLIVDIKLGIFGIIIVLTLLFEPQGLFGIYRRIK
                     VYWKTWPFKY"
     gene            complement(58267..59166)
                     /locus_tag="PLW16_00285"
     CDS             complement(58267..59166)
                     /locus_tag="PLW16_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB69370.1"
                     /translation="MEKILEILLNGLSMGAIYALLALGFVLIFKSTGVINFAQGELAM
                     VGAFICYSFATLMGVSYVYAFFIALIGGLLLGAIVYQVFFRTMVGEPLFSTIMVTVGL
                     ASILACVAGLIWGHDVYSLRSPFTDKIFNLGGIIFAQGSFFTIIVSVILFALFVLFFN
                     RSLLGISMRGTAEDPDTAGLMGINVKRIHMAAWALGALVAAVAGMFLAEQSFVRLSMS
                     HTGIKAMAAAILGGMDSISGAILGGILIGLVEGLAASYLSGMELGNFHFGDIKDVAAF
                     AIMILVLMVRPHGIFGREKVERV"
     gene            complement(59209..61020)
                     /locus_tag="PLW16_00290"
     CDS             complement(59209..61020)
                     /locus_tag="PLW16_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69371.1"
                     /translation="MERVETTLPKLLLEKARLYGGRVAMREKYKGIWQEISWNTYRDK
                     VEAFSLGLVALGMTKGDQVSILGENCPEWIFTDLAIQGVGGVAVGIYPTNSADQVRYI
                     LDHSESRFVVVKDQEQADKVLAAKESLSRLAKIIVIDMKGLRHYEEPLIVSYREVEAA
                     GREAARQDPERFSRLVAATRPEDVAFIVYTSGTTGHPKGAMISHRNIIHQIVNGLQPI
                     LRFTDRDTLLSYLPLCHILERNLSMAMPLVFGYTVNFAESIDTVQQNIQEIGPTFFAA
                     VPRILEKLHSSVQIRMGDSTWFKHWMYRAWQPVGRRVSQFEMSGAPVPWGWRLLYALG
                     YLFIFRAIRDKIGMLNCRCLMSGGAPIAPEILSFFRILGVRTIEMYGITETAGTVSGP
                     RGQIKHGSVGEPCRALEIKLAEDGEILLRGDSIFVGYYRDPAATHDVLPDGWFHTGDI
                     GQLDADGHLYIVDRKKDIIITSGGKNISPSEIENKLKCSTYVKEAIVIGDRRNFITAL
                     IQIDYDNVGNWAQKNKIPFTTYRSLAENPAVYDLIKEEVHAANEGLAQVETVKKFKIL
                     EKELDQDDEELTATQKVKRKIIEERFQKEIEAMYAGR"
     gene            complement(61070..61858)
                     /locus_tag="PLW16_00295"
     CDS             complement(61070..61858)
                     /locus_tag="PLW16_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69372.1"
                     /translation="MAVMNQEASLQLEIADLSMSFGGIQAIRGVSFGVKKGEIYSIIG
                     PNGAGKTTIFNCISGIYRAKTGSIRFESRELIGLKPDRIAQLGISRAFQNIALFKGMT
                     VLDNLMSARVHLMKYGLIGAAVYLGRCLREEVQNRDEVEDVIEFLDLQPVRKSPVGSL
                     SYGLQKRVELARALAMKPKLMLVDEPVSGMNLEEREDMARYIIDINEERDITVILIEH
                     DMGLVMDLSDRVTVLNFGTKIAEGVPGEVSRNPGVVQAYLGEAG"
     gene            complement(61846..62640)
                     /locus_tag="PLW16_00300"
     CDS             complement(61846..62640)
                     /locus_tag="PLW16_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69373.1"
                     /translation="MLRVTNLETTYNDIILALKGISLQVPAGRIVALLGANGAGKTTT
                     INTVSGIRKAIDLKIEDGKVEFDGRDIGALEPHEVVSLGISQVPEGRRIFAELTVEEN
                     ILIGSYALKERKNFIRNEEMVFNYFPILKERRRQQAGYLSGGEQQMLAIARALIAEPR
                     LIMLDEPSLGLAPLVVREIFDIIGHINRERGVAILLVEQNANVALSIAHYGYIMETGR
                     IVMEGPSDVLRENRDIKEFYLGLTDVGTKKSFRDVKHYKRRKRWLS"
     gene            63161..63409
                     /locus_tag="PLW16_00305"
     CDS             63161..63409
                     /locus_tag="PLW16_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69374.1"
                     /translation="MIYIKEKFVDDRTIVMKVDGVLDQDASAVLNHTCQNRLQTKYSI
                     ILNLEGLIHITREGRTFLQQVQDGIRLENIPDFVKLEH"
     gene            63595..66717
                     /locus_tag="PLW16_00310"
     CDS             63595..66717
                     /locus_tag="PLW16_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB69375.1"
                     /translation="METHPVQNDYQRLLATAALFQETFSIDWLMDLFADRKPSEILSL
                     LANGVGEGVLCQEGPGQYRFSHPKKKDGWKQRLSEQEETIAHGFIADILMRQLPDDDQ
                     KAHALAHHLIHIVNDTERCQYLTAAGDLHLNAFRAEDALQCYTKVLDDLSTRHGEDED
                     RLFARTAIQYSKVSTARHDTAQVLAILQAALERAEKRNLQSTQAILKMHMAKNEWLRS
                     RYDSAMHYFDQGWALAKELNEPRLMRSAMNFSTFFLYWQGRFKDAIQIYEQSVSDIEN
                     YPRESFNLLANITIGYCYAQIGQVTQGLGMLDAIRNHCLERGDLYLACHTAGNIGNIL
                     LDIGRREDALSFLETSGREAKKSHNDWVGMSVNINLSYLYYLRGDTKPAVRHLKDFLQ
                     QSKKVQATVYLYPYLLELCLAMKTGKLPQIDGLSLEKEINVLIKGKNIFLKGVAYRFL
                     AIVRKMEGAPPEEIIPILNNAVRWLDEAGHQIELSKTRLELARQYLASGEEDKAQELM
                     QITSKILATFNEEMVPDDLRALIKNRPVGESLLKEIIKLGHEVVTIRDNKDLIQHIIT
                     TLNRITGAERGAIFLFEDGRSPRKLLLRASKNLTQEQISHENFASSMKMIDDVAATGQ
                     GRIFEADLENQALSAGEIIRSRICVPMILRDKTVGVLYHDNRLLSSAFKESDLDLISY
                     FAAWAAFALDNVSAYEEIKRLNRKLCEENLYYKEEHLKNLHFEDIVGESSGIKHVLSQ
                     VEQVAQTDATVMIVGETGVGKELVARAIHRHSARRDKPFIRVHCSALPESLIPSELFG
                     HEKGSFTGAIRRRLGRFELADGGTLFLDEIGDISPDIQVRLLRVLQTKEFERVGGCET
                     LRSDFRLIVATNRNLEGLVKNQTFRSDLYYRLAVFPVYVPPLRERKEDIPRLAYYFLR
                     IYSQKRGKEFSRITEAEMEKLIQYDWPGNVRELENIIERGTILSSGPIFNVPELGAVQ
                     HPETGPRKDVITLRDNERRHILWALEKTAWKVRGKGGAAELLDIPPSTLAFRMNKLGI
                     KRTPAAPVQP"
     gene            66860..68761
                     /locus_tag="PLW16_00315"
     CDS             66860..68761
                     /locus_tag="PLW16_00315"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HPB69376.1"
                     /translation="MSSQLRYAEVYEQSIHQPELFWGQAAEEIQWTKRWDRVLDDSNK
                     PFYRWFAGGELNTCFNALDYQVASGRGEQAAIIYDSPVTGTVRKISYREMLDQVAKFA
                     GVLTAQGVRKGDTVIIYMPMIPEAVMAMLACARIGAVHSVVFGGFAPNELAIRINDAK
                     PRLIVTASCGIEGKKTIPYKPLLDEAVKLADHKPEKCIIYQRPQVTAELTPGRDLAWD
                     AVMADAKAVPCVPVAATDPLYILYTSGTTGRPKGVMRDNGGHAVALKWSMKHIYDIKP
                     GEVYWAASDVGWVVGHSYIVYAPLLTGCTTVLYEGKPVGTPDPGAFWRVISEHGVSVL
                     FTAPTAFRAIKKEDPQGTYLKKYDIRSLRYLFLAGERLDPDTYHWASDMLKIPVVDHW
                     WQTETGWAIAANCMGVEPFPIKAGSPTKPAPGYNVKITDFRGEELPNGQEGYVVIRLP
                     LPPGCMPTLWKDDERFLEYVNKIPDCYVTGDGGYYDEDGYIFIMGRIDDVINVAGHRL
                     STGAMEEIVSKHKDVAECAVLGVQDTLKGEVPLGFVVLKAGSDRDPAEIEQELVRMVR
                     DEIGAVACFKQCVVVKALPKTRSGKILRSTMRKIANGEDFDVPSTIEDASVLGSITDA
                     AQKIGYGKK"
     gene            complement(68988..70532)
                     /locus_tag="PLW16_00320"
     CDS             complement(68988..70532)
                     /locus_tag="PLW16_00320"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB69377.1"
                     /translation="MKLAFANIRDMVETQAHRYGDRIAIRYYERDITYRELDEGSNRV
                     ANGLLKLGVRKGERVCILMDNSPEFYEAYFGIIKIGAIAGPVNCWWQTGEIQYLLNDS
                     GAVALFCDHVYRGHVDKILDGTPALKHVIEREATDDRFLKFEDLRALAADLTPVGIDL
                     DDVATIVYTSGTTGNPKGVLLTHRNILTNSWQASLLANIDENDVIMCFLPLFHVNGLV
                     ITGTSPLSKGSQIILRRNFSASEFWECVAKYRVSIFSGVPTVYQILLNTPGFEGVDVS
                     SLRYGVCGAAPMPVEAIRKFEETFNMIIIEGYGLTEGTAGATANPIDGVRKIGSIGIP
                     FAESEIRLFDEQDREVSQGEVGEICIKGDHVMKGYFNKPEETAQTLRGGWLHTGDMAY
                     KDEEGYLFIVDRKKEMIIRGGENIYPKELEGIIYRHPQVMEVAVVGSPDPIYGEEVMA
                     CLVLHPGQALTEEAFKDWCRANMASYKVPKYVDIRTALPKNILGKILKKELKSALKAE
                     GKITAA"
     gene            70808..71485
                     /locus_tag="PLW16_00325"
     CDS             70808..71485
                     /locus_tag="PLW16_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB69378.1"
                     /translation="MNIIEKALGDGRLALSEYEAKQVLSAYGIPVTREVLVEKEVDLT
                     DALRQVGYPVVMKGCAAAIAHKTEKGLIHVDVRNEAEARQAFSTLREALAETGGAVLV
                     QTFIRGQRELVVGMTRDPQFGPCVMFGLGGIFTEILRDVSFRKAPLERADALEMMQEI
                     KGHKILGALRGMPAVDLEAMAEILIRVGRIGLENDRVKEIDINPIIIVQGKPIAVDAL
                     IVLEPAP"
     gene            71498..72766
                     /locus_tag="PLW16_00330"
     CDS             71498..72766
                     /locus_tag="PLW16_00330"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPB69379.1"
                     /translation="MSTDRFSMREAVRFGWVTTKDHFLFFVGILLAAFLIVIVPEGIG
                     KALSGLADEQAPGLGLAFKGIGLLFSLLSSVLYLILQMGFIRISLNFHDGSPSGFADL
                     FSSWRLFFKYLLGAILYGLRCFWPVFLLGIVAAILIPSLKAQAAPSTTLAAAITLGVL
                     FLILLPLTVIWAIKYQFFGYLVVDRGLGPVEAIRKSADVTDGAKVDLFLFCLLIVGIN
                     LLGMLALMVGLFVTLPLSVIAYAHVYRRLSGEAPVVQRLEDAEAEPDAVVPLDIDLGG
                     GESPVPAAVMKPSVRIVSKTAPASAGEETRTKGLFLLFYRNDYEGAIQEFTRAIGQDA
                     RYAGAYFSRGMAYFKLGEFNLALKDFNEAIDLNRDYAEEAGVFVQRGLAREKLGDRSG
                     AVQDLRAAVALGNRDAQRWLNWIMEQGEDL"
     gene            complement(72790..74037)
                     /locus_tag="PLW16_00335"
     CDS             complement(72790..74037)
                     /locus_tag="PLW16_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB69380.1"
                     /translation="MKREAGLYWGWYVVMGAFLILGINYGARYCFGVFVKPMALEYGW
                     SRSVISVGASLMILTYGIGGILSGRLLDRMAPKWLMTLGALVVSAGFIAMGYIRAPWQ
                     FYLVYGLICGGGAAFFGVVVNSSYVGKWFVKRRGTAIGIATIGIGIGTMLLAPLSGYI
                     VKVYGWRMGFSFLGVLILVIGVLLSQGLMGKTRPEDYGLAPDGDRSGAPPVSAGATVN
                     AAPVGWVLKDSRFWILAACYSLAVMAEMSAFVHQVAYARDNHIEEVVAAASLGFIGIG
                     SIGGRFFFGWLSDHIPDAKYSASLGFICMAAGMIVLLQATSATLLMVYALLFGFGYGS
                     IATMMPYLLADRFGRQVLGTAYGILTFFVAGIGGSIGPVLGGALYDRLGSYAYAWQLN
                     LAVLILAVFLILAMKPRIENPGH"
     gene            74302..76392
                     /locus_tag="PLW16_00340"
     CDS             74302..76392
                     /locus_tag="PLW16_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB69381.1"
                     /translation="MRKSRSPRREPAAGHRVIEADPLLKTVFQRIGKPLEAPLVPDPF
                     QCRAVEAIRHTDCLVTAPTGSGKTWIAEQAIREVFLRGGRAWYTSPLKALTNSKWVEF
                     GKIFGADHVGIVTGDTKENTDAPILVGTTEILRNQLYDAMHSGEDLGCELVILDEAHF
                     LGDRDRGVVWEEIMIYLPARIHLLLLSATISNGEEIAAWLEKIRGKVCTVVNESRRPV
                     PLYPLFLHPKGRIIPLLAKEKLYGQVHEYLHRPHGAGHRHSRPDFGHILEVLKTFNLT
                     PAIFFLKSRSECDAALNSCRRQAPAAVSDPFPLVRDELMDRFPYLANHKQLAHLDGAR
                     VAAHHGGQLPAWKFLVETLMKQGCLDAIFATSTVAAGVNFPARTIVLFNSDLFDGQAF
                     SPMNGTEFHQMTGRAGRRGKDKIGFLLAIPGRFMDLTHVRDLLFKEAESVHSRIRSDF
                     SMVLNLLLSQTPQDIREIFASSFSTFQQKRGPQSGAALAAAERSRAGRRTAPMQTATA
                     DLWEDFLRHLAFLRAEGFVDTDDLLTESGVWASKLRLDQPLLIAECLRRGAFPREDAK
                     LLAAVVAPFVYDGDQDPFPEKERLPRRLVAACNRVARALQPFAARQEAAGFDTHPLCF
                     GAASVIYDWARGSDWDGIIDRTGIGEGDLAMLVLRTADNLRQLASLNETHPEMAALAW
                     NAREAILKEPVRFT"
     gene            76472..76547
                     /locus_tag="PLW16_00345"
     tRNA            76472..76547
                     /locus_tag="PLW16_00345"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76506..76508,aa:Glu,seq:ttc)
     gene            76690..77301
                     /locus_tag="PLW16_00350"
     CDS             76690..77301
                     /locus_tag="PLW16_00350"
                     /inference="COORDINATES: protein motif:HMM:NF022168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrbL family protein"
                     /protein_id="HPB69382.1"
                     /translation="MLDIETARVVGKGLHRVCYAHPQDENLCIKVLLPLESKARLIEA
                     VREVGYYRLLERRGIPWTMLPKFHGEVLTSRGPGYVFDLIRDYDGGVSQTLGHYLASS
                     LETERHAEELAMSFDLLRDYLLQWKIITMTIKAKNILYHRIGKNEGRLVIVDNIGNSD
                     FIPICNYVDFLAERKIRRKWRRFKAALRKEYPKNTFVQRRFNP"
     gene            77558..77695
                     /locus_tag="PLW16_00355"
     CDS             77558..77695
                     /locus_tag="PLW16_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69383.1"
                     /translation="MDELERLRSHITAADFKKGARLGVLYTKLAYNDHTGSQRGTPGT
                     E"
     gene            78116..78613
                     /locus_tag="PLW16_00360"
     CDS             78116..78613
                     /locus_tag="PLW16_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69384.1"
                     /translation="MAFRMALAKRRGFVEIFSWKDNLATRLTLIALFFLGLYISAQWG
                     LWASVAYVLLWVASYLVLFAGTCRRCAYYGRSCPIPLEGSIVCRLFPKSEQPFTGSAI
                     LYASIAYVLRVGLPVGIILQDRLVFEGILLGLIFILFWVSNLFWSGCPNCINDLCPLN
                     PGQGK"
     gene            78667..79149
                     /locus_tag="PLW16_00365"
     CDS             78667..79149
                     /locus_tag="PLW16_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264297331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:78739..78741,aa:Sec)
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPB69385.1"
                     /translation="MSQQHETLLQDTLKKARQRMHLYTUAEASLQGLQALWELPVAAS
                     WATAGYMGAILAGKTTCGTLIGSSIAIGLYCGRHVGAPPEAHPEQRSRSIEGVKQLYA
                     DFIKTFGATDCLALCYCDFSNPGDVTRYIENRAWRESCDRFLDFCIQKCLQMSHDGVI
                     "
     gene            79254..80285
                     /locus_tag="PLW16_00370"
     CDS             79254..80285
                     /locus_tag="PLW16_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HPB69386.1"
                     /translation="MNPLSNSEDVERKILLILRILKESPGPIGARLIARRMQDFGILL
                     SERAVRYHLKLMDERGLTRLVGRRDGRLITEMGIDEAADARVRDKIGLAISRIESLAF
                     RTTFNPQTMNGVLPVNVSFFPRDQFDTALAAMAPAFRRGLAVSDLVAVADEGQRLGEV
                     RVPPDRIGFATVCSIVINGVLLKNGIPMDSKFGGILQVKNGRALRFVELIYYSGSSLD
                     PSEAFILGRMTSVKRVIETGEGKILANFREIPALSRGLAEELFLKLKRAGIRGDLSLG
                     EISEPVGQTPVDVNKVGIILVGGLNPVACAHELGLMTESQAMSTVLDFNDLQNFWTLL
                     DHRNIKYDG"
     gene            80350..81597
                     /locus_tag="PLW16_00375"
     CDS             80350..81597
                     /locus_tag="PLW16_00375"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPB69387.1"
                     /translation="MAELNPFVMAQRQLDQCAKILNLDPGIQAMLRVPVRELHVSLPV
                     RMDDGEVKVFQGFRVQYNDANGPTKGGIRFHPDETIDTVKALAAWMTWKCSLVDLPLG
                     GGKGGVICNPKEMSQGELERLSRAYIDQVWRIIGPEKDIPAPDVYTNPQIMAWMMDEY
                     SKLCGKNQFGVITGKPLALGGSAGRGDATARGGLYTVREAAKVLGLDLAKATIAVQGY
                     GNAGYYAACLAQSLFGSKIVAISDSKGGICCDGGFDPVAVGEHKAQTSSVCSFPETDA
                     ISNEALLEMDVDILFPSAMENVITETNAPKIKAKIIAELANGPTTPEADEILHKNGVH
                     VIPDFLCNAGGVTVSYFEMVQNFYMYYWDEEEVHKRLEKKMTAAYHKVYETSKKYNIN
                     MRQAAYVVAVERVVEAMKLRGWV"
     gene            81908..84877
                     /locus_tag="PLW16_00380"
     CDS             81908..84877
                     /locus_tag="PLW16_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPB69388.1"
                     /translation="MSPVCPESPLRAGDVRHGFLVQRIEAIPDLCVTACELVHEATGA
                     QVVHLQAHDRENLFAIGFRTPPPDSTGLPHILEHSVLAGSERYPVKDAFNELLRGTLQ
                     TFLNAFTYPDKTVYPVASQTRQDFYNLARVYIDLTLRPRLRRETFLQEGHHLEFTVPD
                     DIASDLTVSGVVYNEMKGAYSSPDALMAKVIQESLFPQTPYACDSGGDPDVIPTLTYE
                     AFRAFHRMYYSPSNARIFLYGDIPTEDHLIFLAEMLSGFARVSVDSAIPGQPRWKKSV
                     AVRGTYPIGRDESASGKSVVNVAWMLAENLDGETVTLLEVAADALVGSAAGPLRKALI
                     DSGLGEDLSSATGMERDLRQVAFAVGLRGTDADRADRIEALILDTLERVAREGFARDL
                     IEGALHQVEFHGREIVRGAYPYGIVLMGRAYHTWLYDGDPFAGLNFPRIIGRIREQWS
                     GRSTLFQDIVRQWLIENPHRVLSVMVPDPDDLPRREAAFRERMARLKASLPVARLEEI
                     RTQTEALRKYQTEADSSEALATLPKLQLKDIPRDVETVPTQRTAIAGVPTLRHELFTN
                     GIAYLDLAFDLSHLPEALHPWLPLLGKCTTHMGAAGHDYADMARRITLKTGGLGCGLQ
                     AGLLADDRGHWQKMTFHVRALHRNIEEAVSILIDLLSAGDLEDAARLKDLIAERKNGL
                     QASVIPSGHIFARRAAGAALSLPAYRDEQWHGRTQLKFMHAKAQQGNADPTALQEPLR
                     TLKSTIFKRAGLILNLTADAAGLDGLTAALAPRLGALPAGEDTAPAAPVLPLPHHPGI
                     AIAAQVCYVAKLLPAPAYDNPLTAALMVAARHLAGGYLYKHVRLQGGAYGGLSAYDPL
                     AGVFAFLSYRDPHIVQTLTAYTGAVKTLIDAPVPVEELTKAIIGTIGALDKPLDPSGR
                     GHVAMIREFVGLTDERRQRLRSAILEMTPQGIREAMARYFREAEPRADVAVLAPEDRL
                     RQANQALGNTLVLETLG"
     gene            85091..85576
                     /locus_tag="PLW16_00385"
     CDS             85091..85576
                     /locus_tag="PLW16_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69389.1"
                     /translation="MKRTTALSIRFVITGLILLAIGCAGWPAAQQAGRFDLDEGVTGA
                     FERYEMNPGYDYYISGSDACPNAVLGLEKGISLGETLWKPFPADPAAFKDIIDRMRLR
                     AINLHRHQYGFVLRDGAGQAIGVWYSLISARTVIRRRSDGTFMIYTPDLDTYDLYERE
                     D"
     gene            complement(85602..88235)
                     /gene="alaS"
                     /locus_tag="PLW16_00390"
     CDS             complement(85602..88235)
                     /gene="alaS"
                     /locus_tag="PLW16_00390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPB69390.1"
                     /translation="MMTGTEIRESFLRYFEGKGHARVASSSLVPKDDPTLLFTNAGMV
                     QFKNCFLGLEDRGYKRAASSQKCVRAGGKHNDLENVGVTARHHTFFEMLGNFSFGDYF
                     KKDAIAWGWEYLTGVMALPKDKLWITIFKDDDEAYAIWRDEMGVPAERIVRMGEKSNF
                     WMMGETGPCGPCSEILYDQGEGTGCGRPECDIHCECDRHLEIWNLVFTQFDRDAAGTL
                     SPLPNPNIDTGMGLERLAAVVQGVKSNYDSDLFRPIIRFIERLSGKAYGTGAESDVSI
                     RVIADHSRAVAFLVGDGVLPSNEGRGYVLRRILRRAARHGKLLGFDKPFLHQVCGVVI
                     DLMQGVYPDLTDKRSYIDKVVVNEEQRFSDTLDAGLKILQEEVARLQAGGQGVLGGEI
                     VFKLYDTFGFPTDLTADIVRKEGIRLDMDGFERAMEAQREKARESWKGSGEEAVAEAY
                     QQLSVAGVATTFVGYEGRTEADSRVTALLKKDRPVEEIAAGDSAELFVAETPFYGETG
                     GQVGDTGVVTGDGVQFEVWDTQRPVEGIVTHIGKLKRGRLRVGDAVHLKVEAGDRRAT
                     EANHSGTHVLNAALKAVLGDHVKQSGSLVNPERLRFDFTHFSRIEDDELACIEALANE
                     TIRRNLPVVTKLLPREEAMKSGATAVFDEKYGAEVRVVRMGEVSAELCGGTHVTRTGD
                     IGFLKIVHESAVAAGVRRIEAVTGRGAVVYARRLEEELKKAAGFVKASPFEVSERMEK
                     LLKQQRELEKEIEALKGKLAAKDSADLIDQAREVKGVRVLTAQVEIADVKTLRDFGDK
                     LKDRIGSGIILLGSRSGDKAMLLCLVTKDLTGRYNAGRIIKALAPVVGGSGGGRSDMA
                     QAGGPRPENLPAALNKIEEIL"
     gene            complement(88332..88820)
                     /locus_tag="PLW16_00395"
     CDS             complement(88332..88820)
                     /locus_tag="PLW16_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPB69391.1"
                     /translation="MADTLSDQEALERAFRMLARRPHSEREIQRKLKEKGASGAGIAR
                     VLDRLRELGYVDDAAFARGWAKGRAGGRLWGDRRIAAGLREKGIPAELIRTTLSEIRE
                     DVDEGEALRRCLERKLRGRDPAALELREKRRLAQSLAGRGFPLDLIVRELNAGEGRSV
                     KG"
     gene            complement(88825..89841)
                     /gene="recA"
                     /locus_tag="PLW16_00400"
     CDS             complement(88825..89841)
                     /gene="recA"
                     /locus_tag="PLW16_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPB69392.1"
                     /translation="MVADTERMKAVDLAITQIERQFGKGSIMRLGGGERVADVPAIST
                     GSLSLDLALGVGGVPRGRVIEIFGPESGGKTTLALHMVAEAQKQGGLAAFIDAEHALD
                     VGYAKKIGVDTDDLLISQPDTGEQALEIAETLVRSGALDVLVVDSVAALVPKAELEGE
                     MGDAQMGLQARLMSQALRKLTGSISKSRTTVIFINQLRMKIGVFFGNPETTTGGNALK
                     FYASMRLDIRKVSTIKSGQDVIGMRTRVKVVKNKVAPPFRETEFDIIFGEGISREGDI
                     LDLAAEQGIVEKSGAWYSYKGDRIGQGRDNTKLFLKENTEVMARIEVEVREKFGLKKG
                     EQEA"
     gene            complement(90034..90615)
                     /gene="thpR"
                     /locus_tag="PLW16_00405"
     CDS             complement(90034..90615)
                     /gene="thpR"
                     /locus_tag="PLW16_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPB69393.1"
                     /translation="MSGETTLRAFLAVEPPAAVRGEIAAIQERLKKTVRGVIRWVRPA
                     EIHLTLKFFGNIPPPAVDRISEAVAARTVGVAPFDLTVRTVGVFPDLNRPRVVWLGMA
                     GEVERLLVLQTSLERAFGVLGFPREERPFRAHLTLGRIKTPKGLVGLARAVEIGERAT
                     AGLFRADHLVLFRSELTPQGAVYARLAAFPLRG"
     gene            complement(90641..91897)
                     /locus_tag="PLW16_00410"
     CDS             complement(90641..91897)
                     /locus_tag="PLW16_00410"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPB69394.1"
                     /translation="MRVGILTIGNELISGRTQDTNASLIARTLQIQGWPVVVILSVGD
                     DEAAIREALGLILAQADAAVVTGGLGPTADDITTAAIARIFALPLLTDKAVLATIKSI
                     FEKYRLNWTDNNAKQAVFPAGAEVIPNPVGTAAGFALQKGNQVVAVIPGVPREVARML
                     PEGVVPLLRRAFPEAAACVATRTVKSFGLGEAEVDRALADADWTGMGVEIGFYPHFPE
                     NHIVLTARAATDAEAERRLQAAEAAVTGRLGRHVFSRDGEALEAVVGRLLSERGLTLA
                     VAESCTGGLVTDRLTDVPGSSAYLERGLVTYSNAAKVALLGVPEAVLAVHGAVSEETA
                     RRMAVGVRRQAGTDLGLAVTGIAGPEGGTAAKPVGTVYIALADADETVCRRFAFRWDR
                     RRIKMIAAQSALMMVKRYLAGEVQQA"
     gene            complement(91915..92442)
                     /locus_tag="PLW16_00415"
     CDS             complement(91915..92442)
                     /locus_tag="PLW16_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPB69395.1"
                     /translation="MKAFLAVYARRIPPYRMPEGAPLGERFVRIIATGLGSGYVPFAP
                     GTAGTLVAIPLYLVLFPLSWPLYLLTAVALTFLAIHVSGAAEVLFGRKDAPRIVIDEI
                     CGFLFAMFCVTPTVTHIAAGFALFRLFDIVKPFPAGLIQRRFPGGYGVVLDDVAAGVY
                     ANLVLRALVWAGVLG"
     gene            92632..93954
                     /locus_tag="PLW16_00420"
     CDS             92632..93954
                     /locus_tag="PLW16_00420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPB69396.1"
                     /translation="MNDAILMPHRWPRAILHVDGDAFFTSCEEVLHPELRGKPIVTGG
                     ERGIVACASYAAKALGIRRAVSLPEAKRLCPDLVVLPSDYETYSLLSRRMFNVMRRFT
                     PQVEEYSIDEAFADITGLRRPLKASYETIALRMKDAIERELGLTVSVGLSLTKVLAKA
                     ASKHRKPSGFTVVPSRGIPLLLESLPVGGVWGIGPATASYLDKLGVRTALDFARLAER
                     TARQRLTKPGVETWQELRGTSVYPVSPEEKSVYASISKTKTFAPPSADPEYLFAHLAR
                     NLESACIKARRYGLAPRRVAAFLKRQNFDTSGREARLSRPCAHPLELSPLLRGLFNDL
                     YRRRDLYRATGVVLLDLAPDALIQYSLFEDPLKAEKIRELYEAVDAVSRRYGKHALHL
                     GGSHLIEVRGKGRRGDPTVRENTCPYGKTRRKQLGLPLFRPASGNAET"
     gene            94090..95061
                     /locus_tag="PLW16_00425"
     CDS             94090..95061
                     /locus_tag="PLW16_00425"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPB69397.1"
                     /translation="MTGTKKVFSRFIWIGAALLLAAAAGYALLHRQDQNTVPPGFVVG
                     NGRLEATEIDIATKQPGRLMAVFVREGDAVRPYQLLARMDTAPLEAQLRQAQAEVRRA
                     EEARRSALAKVDESQSRVVLARRELERSERLLAKGIATQQRLEHDQTNRQSAEADDAA
                     ARARVAEAEAAIAAAVARTDYLKAEIADGSLWSPVRGRVLVRLAEPGEVLPAGGKVLT
                     VIDPEEMYMTVFLPEKEAGNLALGAEARVVPDAFPERPLRARVVFVSAKAQFTPKEVE
                     TAEERQKLTFRVKVRLEPHRESRLKPGLPGVSWVRLDPRAPWPPSLQ"
     gene            95058..97820
                     /gene="rbbA"
                     /locus_tag="PLW16_00430"
     CDS             95058..97820
                     /gene="rbbA"
                     /locus_tag="PLW16_00430"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HPB69398.1"
                     /translation="MNDRTDIPPPVCRIAGLRHRYGARRALDDVSLEIPAGRRVGFIG
                     PDGVGKSTILGILAGLKKIQRGNVTVLGGDMRSASYRLQVCSRIAFMPQGLGQNLYTT
                     LSVRENCDFFGRLYNFSRPERDARIRELLSATRLAPFAGRPAGKLSGGMKQKLGLCCA
                     LIHDPDVLVMDEPTTGVDPLSRQQFWKLIDEMRLRHRGMSILVATAYMEEAEHLDWLV
                     MMAGGRVLAAGTPGELKALSGKETIEEAYIALLPEERRAGHRVFAVPPRILSADGAAI
                     EAQHLTKRFGAFTAVEDVSFTIPKGEIFGFVGSNGCGKTTTMKMVTGLLPPTAGEAYL
                     FGSAVDAGSIAMRKRIGYMSQTFSLYGELTVRQNLTLHAHLFDLPAASIPARVEEMSA
                     VFDLGNVMDDQAERLPLGIRQRLSLAVAAIHDPEVLILDEPTSGVDPIARDHFWELLA
                     GLSRERQVTIFITTHYMNEAGRCDRVALMSQGRVLACDSPKELTRRRKCRTLEEAFIA
                     YMREDADGDDREADAGGAGGLERPATILTPPARPESRRRFDIHRLFAVAHKETLELLR
                     DSVLLLFALLVAPLLLLVYGYGINTDIDAIRFAVLDHDGTAASRAYVEHYTGSPYFRD
                     RGQILSPLEMERGFQKLNYSLVIEIPPGFARDLKRERSPAVGVWIDGTMPFRAETAKN
                     YAEAVHLAYLAEQPEAAALPVTGGAVQIESRYWYNPNVRSRLAIVPGVLAAVLLLVPT
                     LLTAVAVVREKELGSITNLYTSPVTRLEFLWGKQLPYVVVNAVNFFALIAIILGVFQI
                     PFRGSFPALVVGALLYLVASTGLGLLVSTFTRTQIAALVAAFVLTMVPAFQFSGLLTP
                     VSSLTGGAAFMARTFPAHYFLDISVGTFTKAIGFQELAAHYLVLAAMCVALTATTVAL
                     LNKQEK"
     gene            97823..98938
                     /locus_tag="PLW16_00435"
     CDS             97823..98938
                     /locus_tag="PLW16_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB69399.1"
                     /translation="MDRIAHIYRLGLKELISIRHEYILIFFLVYSFSALIIVTAKGTG
                     LQVNNASVAIVDEDRSPLSARIRASIRKPYFQTPRDLTFPEIGGTLARNAVTFIIDIP
                     PGFQTDLLAGRRPEIRIHVDGTAIGHAKMGTTYLKKMIAAEISAYLLGERQTPAPAVQ
                     QTVRVRYNPNRQNTWFMGLMMLVNMITMLSIILPAAALLREREHGTVEHLLVMPLSPP
                     EIMIAKVWANSLVVLTGAIFSLWLVVRGVLGAPVAGSFPLFLAGTVVYLFATTGLGIL
                     LATIARNGPQLGLLCVPVITPITMLSGAMTPLEAMPAFLQTVMSVFPTTHYVQFSSAV
                     LIRGAGTAIVWEALAAMALIGAVLFLAALFRFRATFR"
     gene            complement(98959..100314)
                     /locus_tag="PLW16_00440"
     CDS             complement(98959..100314)
                     /locus_tag="PLW16_00440"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB69400.1"
                     /translation="MCDTFVATPDFTASGHLIFGKNSDREPNEAQAIVRYPAAETDAP
                     FVQATYIRIPQVPRTFEVLLSRPFHMWGGEMGVNERGVVIGNEAVFTRVGIDRKTPAL
                     TGMDLLRFALERSASATAALETVTDLLERYGQDACGGYENRRMFYHNSFLIADPNEAY
                     CLETAGRHWAALRVRGFRSISNGLTIESDYDRISGGCIDFARRRGWLKRGATFSFRGC
                     FSDVFYTHLSGSRLRRERTTRAGLAARGRLTAAMAMDILRMEGDPDRTGPFHPARSDM
                     GSICMHATGLFTPSQTAGSLVGEMRPAGPSTCWMTGTSIPSTAVFLPLTLPGTTLMDA
                     GGRMPAARADDSLWWRHERLYRLCLTNYPSAVEAFRREQAELQDAFVRGERTLFDGGA
                     PTAAALDAYSADCLTRVLEAYRAWHDRVAALRLKPAPRAPLYRRRWRRWNRRAGLSLP
                     A"
     gene            complement(100345..101652)
                     /locus_tag="PLW16_00445"
     CDS             complement(100345..101652)
                     /locus_tag="PLW16_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPB69401.1"
                     /translation="MSAPPSDLNAIFAAALAAVDPEPAVRDQLDGVRALYHREGHGRL
                     IVTGFGKAAFAMARAVRRGLPDLIADGCIVTKDGHRVPEVSLDPIRVREAGHPLPDER
                     GLAATAEILGLGAVDERTLVVNLVSGGGSALLVAPAPGVTLTEKQRVTEQLLKAGATI
                     QELNAVRKHLSRVKGGRLAAHLHPARTLSFLLSDVIGDAVDVIASGPTAPDPTTYDEA
                     WAVLERHRLLPDVPDAVRAVLNRGRRGELPETPKPGDPVFSRVENRIVGSNRLALAAA
                     GRRAESLGYAVRILSAELAGEAVEVGKALAAVALRARIEAGSGRDKLCLLAGGETTVT
                     VRGAGRGGRNQELALSFAMEIAGVEGITMLSAGTDGTDGPTDAAGARVDGRTVIRAER
                     RGWAADEYLARNDSYTFFERLGERFVTGPTGTNVMDIQVVLIG"
     gene            complement(101662..102336)
                     /gene="hypB"
                     /locus_tag="PLW16_00450"
     CDS             complement(101662..102336)
                     /gene="hypB"
                     /locus_tag="PLW16_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPB69402.1"
                     /translation="MSDVRLIDIREEILSDNQELAGKIRADLGRHGVFMLNLMSSPGA
                     GKTSLILRTLSTLRDRVRIGVIEGDIASSVDAEKVSREGVPAVQVRTGGFCHLDAPMI
                     DRALKELDLADLDCIIIENVGNLVCPAEFDTGSQRRAMILSVPEGDDKPLKYPLMFSV
                     CDALVVNKVDYLGTSDFDLERLRERVCKLNPAIRIFPVSCKTGEGIAEWTGWLAEQVA
                     SARPPA"
     gene            complement(102336..102686)
                     /gene="hypA"
                     /locus_tag="PLW16_00455"
     CDS             complement(102336..102686)
                     /gene="hypA"
                     /locus_tag="PLW16_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPB69403.1"
                     /translation="MHELAVTESILNVVLRHAGQNGVRRVLGIALRIGELSDLEDTWL
                     QRYFDYLSKDTLAAGARLHIERVPVVFRCEPCRHDFPVNVREIDQVRCPQCGGERMTL
                     VSGREYFIKNIEVI"
     gene            102947..>103232
                     /locus_tag="PLW16_00460"
     CDS             102947..>103232
                     /locus_tag="PLW16_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69404.1"
                     /translation="MKRPLITLLSVGLVLWMAAMARAEVSSVTKIGNDVIVSEGTTVK
                     NATAIGGQVTVDGTIQGDAVAIGGSVVLGSSALVAGDCISIGGVIVKGRGA"
BASE COUNT        19018 a        34071 c        30848 g        19295 t
ORIGIN      
        1 gagcaccaga ctacgaatct gggggccgca ggttcgaatc ctgctgggcg caccacacac
       61 catcaagggg ttggatagtc tcatccaacc ccttgttttt tattgcagat tctgatatca
      121 aaaagactcg aacggtgaac gagccccggc aagcctttaa cagccgcggt ttcgtcccgg
      181 tgcgacccgt ttgccgatgc gtccagcgga gatttcggac accaagaaac gacatgggaa
      241 cctgccggtt cagcatccat ttcggccgtc agttccggcg tccgggagaa catccaaatc
      301 aatccacctt tctttttcgg ggaggaccgc gatgaaccag gcaaaagcag attccgatac
      361 aaagccccgg atgaaaatca atccaccggt ctttttcggc gccgcggggt tgatcgtcct
      421 ttttgtcgtc ttcaccatca tttggcccga gcggacgggg gcgatcttcg gcagtgtgca
      481 aaactggatc gtacattccg ccggctggtt ctacgttctg gcggtggcca ccttcctcat
      541 cttcgtggtg tttctggcca tctctgattt cgggcgcatc aagctcggtc ccgaccacag
      601 cgagcccgac tacagctacg ggtcctggtt tgccatgctc ttttcagccg gcatgggtat
      661 tgggctgatg tttttcggtg tggccgaacc ggtgatgcat tacatgaagc cgccggtggg
      721 agatcccgag acggtgcaag cggcccgtga agcgatgaag atcactttct tccactgggg
      781 ggttcacgcc tggggaatat acgccgtggt cgccatttcg ctggcctatt ttgcctatcg
      841 gcacaacctg ccactgacca tccgttccgc cttttacccg cttatcggtg atcgcattaa
      901 cggcccggta ggtcatgccg tggacatctt cgcaatcctg ggaaccatgt tcggcgtggc
      961 gacctcgctg ggcttcgggg ccatgcaggt caacgccggc atcacgtatc tgatcggcgc
     1021 tcccaacaat gtctatgtac agattctttt aattgccgtc atcaccggta ttgcgacgat
     1081 atccgtcgtg ctggggcttg acggcggcat tcgcaggctc tcccagctca atatgttcct
     1141 cgctgcgggg ctgttgatct ttgtccttat tgtcggcccc accgtctatc tgttacagac
     1201 gctcacccag aacatcggcg cgtatgcttc ggacctcgtc aataaaacct ttaacctgta
     1261 tgcctatcag cccagcggct ggatcggcgg ctggacgctg ttctactggg gttggtggat
     1321 tgcctgggcg ccttttgtcg gcatgttcat cgcacgggtc tcacgggggc ggaccatccg
     1381 ggaattcgtc acgggtgtct tattgcttcc ggccggattc acgttcatgt ggatgacctt
     1441 tttcggaaac acggccctgc atatgatcat ggttcagggc gcaaccgacc tggggaatgc
     1501 cgtggctgcc gataccacga tggccctgtt caatttcttt gaacacctgc cattctctgc
     1561 aatcctatcg tttctggcga cgattctgat tgtgaccttc tttgtgacct cttcggattc
     1621 cggttcgctg gtcatcgaca tgctggcctc cggcggtgaa gacgacccgc cggtctggca
     1681 gcgcatcttt tgggccacga gcgaaggtct ggtcgctgtg gcgctgcttc tggccggagg
     1741 actgggggcg cttcagaccg cggccattgc cagcgcactt ccctttacca tcatcatgct
     1801 gctgatgtgc tggggactgg tgcgcgctct gcgcgtggac gtggccaaac ggatctcccg
     1861 gcgcagcgcc gtgctgatgc cgcagaccgc cgggaaaccg gtttcgtggc agcgaaggct
     1921 gcagaatgtt ctgcgtcatc ccgtcgagtc ggaagtgatt gattttatca acaccacggt
     1981 ccggcccgcg ctggaagacg tcgcggagga attccggctc aagaaaatgg acgttgaagt
     2041 aaaacccggc gaggacggcc ggatgtggct ggaagttctg catggcgaag aacacaactt
     2101 ctattacggc gtgcggccgc gcacttacac accccctgcc ttcgccatgc gcgacacgca
     2161 aaaaagacgc ggggaggcgc tcaagttcta ccgagccgaa gtgtatttga gcgaaggcgg
     2221 tcaagactac gacctgatgg gctggagcaa ggaacaaatc attaccgacg tcatcgatca
     2281 ttatgaaaaa cacatgcact ttttgcacct catgcggtaa gaaacgcagc aagacgaaaa
     2341 gagccaaccg gcaacagccg gacgagaaat ggcggtccct gtgcgccggg gcggtcggct
     2401 ttctcgccgg acaggcaatg cgggcgtcca ggggagaggc gaacccgaag cgggcccggg
     2461 aaatcctgat ggaacgactg gataagggct ggtgaagatg gaacaaacac cggaggtcgg
     2521 acgaaggaga acgttttacg acgaggcacc ggactaccgg cgcccactcc tggagattcc
     2581 ggcggatcgg aaggcccgga tgcgcgagcg gctatcttgc ctctacggag atacggccgc
     2641 cggggagtgg ctgcccgaac tggaacggat catcaaggtg cacttcgccc acaagtctcc
     2701 ggaaatgatc gaaaaggaga aggcttacga cccccgggag cgcttctcca acgatcatct
     2761 catcctgatc acctacggcg acagcgttcg gggggacgga ccgacgcccc tcgcgtcgct
     2821 gcgtcgattt atcgacacct tcacccgtgg cgcgatcaac acgttgcata tcttgccctt
     2881 ctttccctat tcctcggacc gagggtttgc cgtcatcgac tttcgccggg tggatgacaa
     2941 gctgggttcc tgggcggaca tccgcaagac gaagcagcgg tatgacctga tgttcgacgc
     3001 cgtgttgaac cactgttcgt cgcgaagcag gatgttccgg gagtttctca atggcaatcc
     3061 cgtctaccgg gacttcttta tcgcctatga ttcgcccgac gacctcaccc ccgagcagcg
     3121 agggctgatc tttcgccccc ggacgtcgga tatcttgacg cgctttatga ccatcgacgg
     3181 gccgaagtac ctctggacga ccttctcggc cgatcagatc gacatgaact tccgcaatcc
     3241 ggccgtcctg atggcggtga tcgacagcat cctcttctac gtccggcggg gagcggatct
     3301 gctgcgcttg gacgcggtga cctacctgtg gtctgaaccg gggaccgaat cggcccacct
     3361 gccccagacc cacgagatca tcaagctgtt gcgggacgtg gtggacgtcg cggcctccgg
     3421 ggtagccctg gtcaccgaga cgaacgtgtc tcaccgtgaa aacgttgcct atttcggaga
     3481 cggccgagat gaggcgcacc tggtgtacaa ctttgccctg ccgccgttgg tcctgctcgc
     3541 cttctatcgg ggcgatgcca ccccgctctc acagtgggcc cagggactgg aaccgccgtc
     3601 ggaccggacc gccttcctga atatcctcga tacccatgac gggatcggcc tgatgggcgt
     3661 tcgagggatc ctcccggagg aggagatcgg cttcatcgtc gaaacggcgc gggaaatggg
     3721 agccctggtt tccggaagaa acaatcggga ggcaacggaa gaaccctacg aattgaacac
     3781 gacttggtgg agtgccctca accgttccga cgccggcgag agcctggatt ttcaggtgcg
     3841 ccgcttcctg gcctcgcgaa gcatcgccct gacgctcaag ggcgttccgg gaatttacat
     3901 ccacggcgcc ctcgggacgc ccagtgattt ggacgcggct gaggcttcgg gggagaaacg
     3961 ggatgtgaac cgcgccatcg tcgacaccag gacgttgatg gcggaccttt gggacccggg
     4021 gtccaaactg tcccttctgc tccgctacgg gacccacccg atccaggtca ggcggaggga
     4081 acgggccttc catccccggg gcgcccaacg agttctcttg ctctcgccga gggttttcgc
     4141 cctctggcga acctcccccg aaggcgaaga gtccgtgctg gccatcacca atgtgaccgc
     4201 aacaacggcg gccgtggaaa tccccgttgc gtcgttagga atggcgggca aggcctggaa
     4261 gagcctcctc tccggtcggg aatggctgcc cgaaggcgat acctggaccc tggcactgaa
     4321 gccttacggc gtggtctggc tcaaacccgt ggaaccgtct ccgtaaccgg atgtccctct
     4381 ctcgctcggc gtcccgctta cccttgaggc gtcttgatca gatgaaatcc ccggttgatg
     4441 agacaatggt tggagtgggt ggagttgcgg cgcgccgacc ggatggatct tcgtcaggtt
     4501 cccccaaaag tagcgtttcc cggcacaata cccccaggag gctctaatga agaaagcacg
     4561 atttaggaga cgcagatctg ccggcattct caaggaatct gatgctggaa ctccggtcaa
     4621 ggagacctgc cgcacccatg agatctctga tgccaccgtg cttcgatttc caaggggaga
     4681 ggacgttccc attcgctgtc ggggaacggc ctctcaagaa aaggggggct tccgactatt
     4741 tcttgacggc aattttgttt ttgacggatt tgacgccggt cacggccttg gcaatcgtgt
     4801 ccgccgtttc cttcgcctgg ggggttttca cggttcccga caggctcacc acgccttctt
     4861 tggtttgtac cttgattttt gaacccttca aggacgtatc cgcgaagaga ttggccttga
     4921 cctctgccgt aatcgagctg tcatcgacaa cggcattcgc ggcgtccttg gcgacggtgc
     4981 tcgccttgtc tctggctgca gatgtgacgt ctttcaggat ctggccatgg gcaaccgaaa
     5041 ccagggccag acaaagaatc cccgcgatga tgaatcgttt catatatccc tcctcaaggt
     5101 atttgtcatc cgtataatga acccgaagcg ttctgtcaag gggttgccat cggtcgtcgt
     5161 gccggcctca ttttttgggg gtcttccccc cgaacggtca ggaaaccatt tccgcaacga
     5221 tcgccttgac cggcttgatc tcctggatca gcgccgcgat ttgccccgcc cccgccggga
     5281 agagatcgaa gtctcccgtg gtccaggcct tcggggcatt ggcaaacggg aatgccctgg
     5341 agacatccgc ggcgggatcg gccaggagat cctgcatctt cttgttcacg atcactcgca
     5401 tcttcgtgtt catctccctc acgggaagca gagacgtacc gccgtcacgg caggccacga
     5461 tggcttcctt ccatgcctgg ggggccggac tctcttccga agcaatcaat cgcgtcccta
     5521 tctggacgcc ctgcgccccg agtgcaaggg ccgcgcgata gccgcgcgcg tcggcgattc
     5581 ctccggccgc gacgatcgga acggcgacat gatctgcaac gagcgggatg agaaccatgg
     5641 tggatgattc ggaggcgacc gagcggatcc cgccggattc cgcccccgac accaccagtc
     5701 cgtccacgcc atccctcgat gccttgacgg catgggggag cgaaaggaca acatgaaggc
     5761 cgtgcatgcc caaggcatgg atgaggggat agattttcga cggcgccccc accgaggtcg
     5821 tcaccatcgt gacgccggcg tccttcagga tctcgagaat ctccgcgttc gagggattca
     5881 tgatcatgag gttcgccccg aacggcttgt ccgtcaggcc tctaacctcg ccggctaatt
     5941 tttttgcctc ttcgggaccg gagaacccga gggcaagcat cccgaaggca cccgcctccg
     6001 aaatcgcggc caccagccgg ggactgttca gggcgcctat gggcccctgc atgatcggat
     6061 actcgcaccc caacatctcc cgtaaaccag ccattctcgc cctccttttc cggtcgcatg
     6121 tttgaaaaaa aatcaccccg atgccgaatc tacatgacag ccatggcgcc gtcaacaagt
     6181 ttgaggggga cgtcacggac acgtcagaat ctttcctctt tcaactttat cggaaatcgc
     6241 gtagataaat atgcagaaaa acgggattgc aggggccgct gcgggagggg attacgttgc
     6301 tgtccaaaga ctatgtcgag gcccgggagc tctccgaggg cattgcccgc gcggtgggat
     6361 cgccgcgttt ctacatcgaa cgtcatgccg agcttgagcg atcgcaagcc ctgttcgacg
     6421 gcgcgcccct ggtggagaag gcgctgcgcc tcgccgccgg ctacggagac tgtctggggc
     6481 acggacagtc gcacgtcagg aaggtcgcca tcgacacggg ggcgctcgtc ctgatcgagg
     6541 gggcgggcga cccggatttg ccgcggcttg tcctgctggc ccacctggcc gggctcctcc
     6601 acgatatcaa gcgctccgag aaagaccacg cccatcgcgg ggccgaagag gcggccctaa
     6661 tcctgaagac cttccccctc cggatggcgg aaaaccgggc catcacccag gccatcggca
     6721 accacgaggc cttccggacc tacgagaccc tggccgatcc gagggccgaa ctgttgtcca
     6781 acgccctgta cgacgcggat aaattccgct ggggaccgga caacttcacc gaaaccgtct
     6841 gggccatggt cgccccgcgg cgcatccccc tacgggtgct gatggcccac ttcctccccg
     6901 gactggaggg cgtccgacgg atccgggaga ctttccgcac ccccacgggc aggcggtacg
     6961 gaccggattt catcgaccgg ggtctggaga tcgggcggcg cctgcacgaa gcgctcatgc
     7021 aaaaaatcga cgccgagggc gtggaggcgt ctcgcccacc ccgagggact ccggagaagg
     7081 gataggacgc catgcattca ttgaccctcc gaaccgcatc ccggttcgag atgaccgaca
     7141 tcacggacag gatcaacggc atgatcggcg aaaacggcat cgtcagcggc atcgcggtca
     7201 tgttcgtccc ccacacgacc gcggccgtca cgatcaacga gaacgccgat cccgacgtgc
     7261 ccagagacat cctggcctcc ctggaccggg ccatcccgct gaaggatcgc tacctccatg
     7321 atgaaggcaa ttcggcggcc cacgtcaagg cttcgctcgt gggaagctcg gaaatcgtcc
     7381 tgatcgaggg gggacgcctg gccctgggca cgtggcagtc ggtgttcttc tgcgaattcg
     7441 acggtccgag gacgcgccag atctggatca agctggcagg aacaagctga gacggaatcg
     7501 gggcccgtcg cggatgaggg gaggattcca cccccgtcct ccatccggaa tcgggcggcg
     7561 acggggggag agcgatccgt ccctcgtgtt gcggatttac tgggatttga cggagatctt
     7621 tcggtctgct ttcctcgcct cctccgtttt cggaagggtg acggtgagca gaccgttctt
     7681 gaacgcggcc tcgactttct tggcgtcgat actcgccggc aggggaatct cacgatggaa
     7741 ggagccgtag gaccgctcca tccggtagta acccttgccc tgatcttcct tctcctcctt
     7801 cttttcgcct ttgatggtca gggcatgctc gctcagagac acgtcgaggt ccttctcgtc
     7861 gatcccgggc agctccgcct tgaccaccac ctccttttca tcctccttga cgtccacgaa
     7921 gggcgtgaac cccatcagcc gcccctccat ggcgccgacg ggcgccgcat cgaacccgcg
     7981 gaaaaaactg tcgaacagtc ggttcatctc ctgctgcaag gcgtcaaaag gatgttccgg
     8041 ctcccgtcgc gccggggcgc tgccgcgcca gagagacggg actaggtttc tgatcggcat
     8101 cgtcattatc ctcctttcgc aaaagatcct gtgttttacg ctgccttgat ggcaatcttc
     8161 ctcgccttca cggcctcggc cttgtgcaga acgactctga gaaccccgtt tttgacggtg
     8221 gcctcgatcc gctcccgatc cacttcatcg gaaatcgtga aggcgcgctc atagtcgccg
     8281 gcatcatatt cggcataggc cagactgtag cccgcccggg ccccattggc ggcctggccg
     8341 acgagggtca ggatgttctt ttctagggtg atgtccaccc ccgtctcatc cacccccggc
     8401 atgtccgcca aaaggacgat cgcctccgag gtctcgtata tgtccacccg cgggacgaag
     8461 acgcgcctgg cgcgggtgcg ttccgttccg gccggagttg ccgccggttg cttgaccatg
     8521 tctttcttgt cttctgccat gatccgttac ctcctttcct caggacttga tcgatatctt
     8581 cttcggcttc tcggcctcgg agcgtgggag aaccacgttc aggattcccc gctcgtaggt
     8641 cgccgcgacc ttggccgcgt cgacgtgaaa ggggagctga atggtccgcg tgaagcggcc
     8701 acagctgcgc tcctgccgat ggtaactctc cccggccttc agggcctccg cttcccggtt
     8761 cccgctgatc gtgagactgt cccccaccac ggaaatatcc agcttctcag gatccgcacc
     8821 gggaagttcc gccgtcacca ccatgccctc ttccccggcc caggcgttga tcgcgggaaa
     8881 ctcctggccg tagggctgcg aggcactcga aaaaattcgg ttcatctcct gctgcaggcg
     8941 ctggatttcc ggccagggat ccatggcgcg tccgaatctc cataaaacag gtccgtacat
     9001 cgttgcacct cctttaaaag aagtttgttc acgtttgagg cgggtcgcgg gcacacgcag
     9061 cccgtgacat tcctctatat aaccttccga tatcgtttga tcatttccga tttgtcccta
     9121 atatagtcac caaatatcgg ttgtcaagag aggagggtgc aggacaggcg atgcggccct
     9181 ttcagacacc tcgccgtggg ccgggaagga gaatcggagg ggttcaggtc tgactcagga
     9241 gttccagcag gtcggggtga atcctgccgt tggaggcgag aatgtgctga gcatgcaagt
     9301 gataggggcc gccgtcgagg tcggtgacgc gcccgcccgc ttcctcgacg agaagccatc
     9361 cggccgccgt atcccagggc gccagtttga gctcccagaa gccgtcgaac cggccggccg
     9421 ccacgtaggc gagatcgagc gccgccgagc cggcccgccg gatggcctgg gccttgagcg
     9481 ccagcgtctc gaaatgatcc agattgttgt cccggctctc ccggatgtcg taggggaatc
     9541 ccgttgccag gaggcttcgg gtcagggcgt ccgtccggga gacggacagg gcccggccgt
     9601 cgagaaaggc gcctccgccc ctgagcgcgg agaagcattc gtccagcatg gggttatgga
     9661 ccacccccag gacgatctgt ctccggcact ccagggccac cgatacgcag aagacgggat
     9721 agccgtgcgc gtagttggtc gtcccgtcca acggatcgac gacccagcga tactcggcgc
     9781 cggtgcccgc atccgcggat tcctcggcca ggatgtcatg atcggggaat cttgcccgga
     9841 tgcgttcccg cagatggttc tccgccagca ggtcggcttc cgtaacgatg ttgatgtctc
     9901 ccttgtacgt gatccggtgg ggggtcgcaa acgcaccctt caagaggctt cccgcctccc
     9961 gggcaatggt tacggcaaag ttcagcatat ccatcatcgg cggcacccct cttcaatggg
    10021 tctggcccgt ctcccccgcg gcgcatcctc tataccacat cttcccgaag acgcaatccc
    10081 ccccgaggtt tttattgcgc ggacggggga atcatgataa tttcccggcg caaggttttg
    10141 gcgacggaat gacgcaggtc ggacaggaga cggacgtgga agaagccgac gacaagagcg
    10201 ggtcgggcat ccttgatcta aaggccctcc gggaatcccg gggtctcacc ctggagaacg
    10261 ttcaccagcg gacgcggatc agcatgacca acctggaagc cctggaggca ttccggttcg
    10321 aggcgctgcc gatccccatt tacacccggg ggttcctcag actctacgcc caggccgtgg
    10381 gaatcgatcc cgcgccgctt ctggagcacc accggcagta cctgaacgcg atcagcacca
    10441 ctgcgcccac ggaggttccc gccggccgcc cgctgaagcg cctccccggc aaaacggtca
    10501 aacgcctcct ctggtccgcg gcgctgatcg tgctcgtcgg ggtgctgttc attgccctga
    10561 ccgcggaaca gcgcaccccc ggccttgccg ggaagacggt cgaaagatcc gccgacctct
    10621 ctccggatgc cgggagcgac gcccccgcgc cgggaatgca aaaagacccg caagaagcgg
    10681 gcatgatcaa tccggtcatg cctgaaaacc gcgtctcgac cccgacggcg ccggcagacc
    10741 cgaagccccc ggccgcggcg gtcaccggac aggcggttcc ccccgatgac cccgcccgta
    10801 tcgtcaaggg ccaagccccc ccttaccggc tggccctgga ggcccgggaa ctgacttgga
    10861 tccggatcac ccaggatcag gagcagccct atcagatttt actgaacccc ggcgaccggg
    10921 tggagcgggt cgccttacag gggtttaccc tggatatcgg aaatgccagt ggcgttgccg
    10981 tcgtgttcca gggcaggtct ctgggggaac tcggcgatcc gggagaggtg gtgcacctga
    11041 gacttcccgc cgctccctga gatccggccg gccgttgcat cccccgggcc ggatttcttc
    11101 gctgttgaca aaagcggaac ggatgtttta aggttgaatg cacccgtaag gacggcgcgc
    11161 ttttccgagg aagcggccga tcaggtgcgg caggtcatca attgtccatg aaggagacgt
    11221 gagccatgtt cggaatcggc atgcccgaat tactgattat actcgtcatc atcctgatca
    11281 tcttcggggc cggcaaactc ccggaaatcg gcggcgcgat cggcaagggc atcaagaatt
    11341 tcaagaaggc ctcccgggag ccggaagaga tcgacgtcac accggacgcc aagaagatcg
    11401 acgagaagaa accgtccgcc aacccctaaa agggaacatc ctcatccgga gcgggaccga
    11461 acgccgaggc cggcgcgggg gcctcctcac cgctctccgg tcccttgccc gtgcccttgg
    11521 aatccagcat ccgcatgtcc gaggcgatga tctccgtcgt ataacgtttc acgccctcct
    11581 tgtcctccca ggcattggtc cggatccggc cttcgatata gaccatcttc cccttggcca
    11641 ggtagttccc gcagatttcc gccaacttgc cgaaggtcac gatgcgatgc cattcggtga
    11701 ccttgacccg gtcgccgttt ttatccttcc gctgctcatc cgtcgccagg cgaaagttgg
    11761 ttatattcat cccatccggc gtataccgca cctccggatc cgcgcccaat ctccccacca
    11821 gaatcacctt gttgaccatc gcgttcctcc tttcatccgt ttggcgaacg gcgacgcgtt
    11881 gcgcgtcgtc cgcgccggcc gttctgcgct cgaacgcctt cctgatacac aagttttgcg
    11941 ggattggcaa tgcttattta cacaaaacat gcttgactaa tgcaccgatt caatatagtt
    12001 aatgcgactt tgtacgcagg gggcaaattt tccatggaag acagcgaatt actacggaag
    12061 cgccgggaaa agatcgatgc cctgaaggct gacggggtgg acctctatcc caacgatgtc
    12121 cgggtggacc atacgacggg ggagatccag gaccgtttcg gccacttgga cgaagcggcc
    12181 ctccaggatg tcccggagac ggtttccctg gccggacgca tcatggcgat ccggaacttc
    12241 ggaaaggcgg ccttcgccgt tctccaggac cgtaaaggcc gccttcaggt ctacatcgcg
    12301 cggaatatcc tcggtgacgc tccctacacc cttttcaagc gcctggacat cggcgacatc
    12361 gtcttcgtcg ccggccgggt gttccgcacc cggaccggag agttgaccgt ggaggcgcac
    12421 gcgttgcgtc tgatcgcgaa gtccctgcac cccctgcccg aaaaatggca cggcctcacc
    12481 gacgtggaaa tccgctaccg gcagcggcat ctggacctga tcgtcaaccc gaaggtgcgc
    12541 gacgtctttt acaggcgcag tcggatcatc caactgatcc gccgcttcat ggaggatcgg
    12601 gacttcctgg aggtggaaac ccccatgatg cagcccaagg ccgggggcgc cgtcgcccgc
    12661 cccttcaaga cgttccacaa cgccttgggg atggatttct atctgcgcat cgcgccggag
    12721 ctctacctca agcgcctgat caccggtggc ctcgagcgag tcttcgagat caaccggaac
    12781 ttccggaacg aggggatgtc ctccttccac aatccggaat tcaccatgat ggaattctat
    12841 caggcctacg cgaccttcct cgatctgatg acgctgacgg aagacctctt cacccatatc
    12901 gcccgtgacc tcttcggagg cctgaccttt acctaccagg gaaccgacat cgatctgacc
    12961 cctccctgga cgcgcctcac ggtgcaggag gccatcctgc agtacacgga gctgcccccg
    13021 gaggtcttgc gggatccggc ccgggcggtg gcccaggccc gcgcccgggg tctggacgtc
    13081 aaggacgacg accctctggg caaggtcatc atggcgatct tcgaggaaac ggtggaaacc
    13141 cggctcgtcc aaccccactt catcacccac tacccggtcg aggtttcccc gctgtcccgg
    13201 cgcagcgtcg acaaccccgg cctgaccgac cggttcgagc tttatatctg cggccgggag
    13261 atcgccaacg ccttttccga gctgaacgat ccggcggacc agcgggaacg ctttctcatg
    13321 caggcgaagg atcgggatgc cggggacgac gaggcccacg tcatggacga ggactacgtc
    13381 cgggtcctgg aatacggcat gccccccacc gccggcgagg ggatcggcat cgaccgcctc
    13441 gtcatgctct tcaccgacgc gacatcgatc cgggacgtga tcctgttccc ccacatgcgg
    13501 atcaaggaag acgcataaca tgtcctatga gctcttcatc agccggcggt acctgcgggc
    13561 gaggcggaag caggttttcg tctccatcat caccttgcta tccatcgccg ggattcttct
    13621 gggcgtggcc gccctgatca tcgtcctcgc cgtcatgaac ggcttcgaga cggatctgcg
    13681 ggacaagatc ctcggaatca actcccacat cgtgctcatg gagtacagcg gcgtcatgcg
    13741 cgatcccgac cgcgtcctgg agagggtccg ccggacggag ggcgtcgccg ccgcgacccc
    13801 cttcatctat gcccaggcca tgctgaagag cggtccacgc gtcagcgggg tcgtcctgag
    13861 gggcatggat accgccaccg cctcccaagt catcagcctg ggccggatgc gcgaggggaa
    13921 actcgcctac ctcgatgcgc ccccgccccc cgcgtccggg ctgtccggag atctccgggg
    13981 gctgccggga atcgtcgtcg gaaaggaact ggccaagaac ctgggactct ttctgtacgg
    14041 gcctctttac gtcatctcgc ccgcgggaac ggccacgccg atgggcatgg cgccccggat
    14101 gaagaagttc gtcgtcgtcg gcatcttcga ctcaggattc tatgaatacg actccacgct
    14161 cgcctatgtg tcgctccggg acagccagga gcttctccag atgggcggga gcgtcacggg
    14221 gatcgagatc aagatccggg acctgtaccg cgccgacgcc gcggccaaac ggatggagca
    14281 ggcgctggga tatccctact gggcccggca ctggatggag atgaacaaaa acctcttttc
    14341 cgccctcaaa ctggaaaagc gcgtcatgtt catcattctg tcgctcatcg tgctggtcgc
    14401 ggccttcaac atcatcacga cgctcatcat ggtcgtgatg gaaaagacga aggacatcgc
    14461 cgtgctgaaa tccatggggg ccacctcgcg gagcatcatg cgcatcttcg tctatcaggg
    14521 actcatcatc ggggccgttg gaacgctgct tggatgcgcc acgggcctgg ccgtcgcctt
    14581 caatctcgag aaaatctccg cggccatcga aacccttttt ggattcaaaa tcctgcccgg
    14641 cgacgtctac tacctgagca cccttccctc ccaggtgaat tacggcgatg tggccgtcat
    14701 cgtggcgggc agcctgctca tcagccttct ctccaccctt tatccgtcct atcgcgccgg
    14761 aagactcgac ccggcggaaa cgctccgata cgagtagaat atggcgatcg cgattcaaaa
    14821 cctgtggaaa acctatatca aggacggcaa ccggatcgac gccctcaagg gcatcgacct
    14881 cgacatcgcc gccgccgaat cgctggcctt agtcggcgtc tcgggcgccg gcaagagcac
    14941 cctgatccac attctgggga cgctggatcg ccccacgcag ggacgggttc tctataacgg
    15001 cgtcgacgtt ttcacctggc ccgagaagcg gctggccgcc tttcgcaaca cccggatcgg
    15061 gttcgtcttc cagtttcatc aactcctgcc ggagttttcc ggtctggaga acgtcatgct
    15121 ccccgccctg atcggcgggc tcgcgaagcg ggaggcccgg aaaaaggcgg aaggcctctt
    15181 gaaggatgtg ggacttggtg ataggatgac gcacaaaccc ggcgagctct ccggcggcga
    15241 gcagcagcgc gtcgcggtcg cccgggcgct gatcatggcg ccggatattc tcctggccga
    15301 tgaaccgaca ggcaatctcg atacggaaac gggcaaaatc attgaggacc tgctcatcga
    15361 tctccacaga gaacggaacg tcacgctcgt cgtggtcacc cacaaccaat ccctggccca
    15421 gcggatgtcg agaagcgtcg gcctgcgcga cgggaggatc gatccccgtg cctgagaaac
    15481 cgcatcccct cgctccggcc tgccgtctgc tgctgctcct catggcgctt ctctgcctct
    15541 ggtcggcgga gacatcggca gaggaaggcg tgaagtcggg tccgaagaag gcggccctcc
    15601 tgcccttcgc cgtccacagc cggtcggacg ccgccgggct ccagacggcg atcgggaaag
    15661 ccctggacgc cgaactgcgc aaatcctccc gctacctcga actcatcccc gggtcggcct
    15721 tcgccccgga gatcgagggg aaacccctcg atgacgccct ggccctccgc gtggggcaga
    15781 ccaccggcgc cgatgtcgtg ctccggggca gcctcacgga gttcggcgaa tcggtcagtg
    15841 tcgatgtcag cctgcttttc gtccggtccg gcaaatccgt ctccggcatc tccgcccagg
    15901 ggaagggccg cgaagacctc ccgcggatgg ccagccaact ggcggcggat atcctcatcc
    15961 ggacgtcggc tgccgctcgc ctcgccaaaa tcgacatcca gggaaaccgg cgcatcgaga
    16021 gcgacgccat ccgtcaagca gtcaagagca cggcggggca actgctctcc gacgaaacgc
    16081 tttccaggga cctcaaggcc atctaccgga tgggctattt cgaagatgtc caggccagcg
    16141 tggccgacgg accggaaggg aaggtcctca ccttcacggt ccgggagagg cccatgatca
    16201 cggaggtccg gatcaagggc aacaaggcga tcgacaccgg tgatatcgag gccgtgctcg
    16261 gcgtgaagac ccggcagatc gtcaaccgcg aaaaggtcaa ggaggacgtg gaaaagatcc
    16321 gggccctcta tatcggcaag ggctactaca acgtcgagat tgcggatgcg gtggagggcg
    16381 acggggacaa ggaggtgcgc gtcgtcttca cgatcaccga aaacaagcgg ctcttcatca
    16441 aggccatccg cttcgaaggg aaccaggcct tcacggaaaa acagctccgg aacatgatgg
    16501 cgaccaacga gtggggcctt ctccatttct tcaccgattc cggggtgctg aaaacggacg
    16561 tcctcaagca ggacatcggc aaactgaatg cgttctatct gaacaacggc tatatcaacg
    16621 cccagatcgg cgagccggaa gtcacccacg acgccaaagg aatctacgtc aagatccgcg
    16681 tggtcgaggg gaagcccttc aaggtcggca atgtggagat tcatggagac ccactgcaga
    16741 cgccccgtga agcgctccgg gagaagctcg ccatcctggg gaagacgtac tacaaccggg
    16801 aggccatcgt ccgcgacatc gacaccctga cccgggtctg caacgacgaa ggctacgcct
    16861 atgccgacgt cacgccccgg acgaaggcgc aggaaaagac ccagaccgtc gacatcgtct
    16921 acgagatcgc caagggcaaa caggtctact tcaaccggat ttcgatcgtc ggcaacaccc
    16981 gcacgcgcga caaggttctc cgccgccagc tcaacatcgt ggaaggagac ctttacaaca
    17041 gcagcaagat gaagcagagc tacatggccc tcaaccgact gcgctatttc gaggaggtgg
    17101 acttccagtc ggaaagaggt cccgacgaca ccctgacgaa cgtcatcatc cgcgtcaagg
    17161 aaaagcccac gggcatgttc agcatcggcg ccggctacag cgcccaggac gcggcgatgg
    17221 tcatggctca ggtctcccag cagaacctct tcggccgggg ccagatcctc tcgctcaagg
    17281 ccaacatcgg gtccgagacg gcgatgtacg aactgtcctt caccgaaccg tggctcttcg
    17341 acatccccct gtggagccag ttcagcctct ggaatttcac gcgggaatac gacacctacg
    17401 acctcgattc cagcggcgtc ggcgcgacgc tggggtatcc cctgtgggag cgcatcaccg
    17461 gctatctcgg ctaccggctg tccatcgacg acgtctacaa catcgacgaa gccgacgcct
    17521 cgtactacgt caaggagcag gcgggagaga caacgacgag cagcgcctcg ctcaccctct
    17581 cccgggacac gaccgacgac accctgtttc ccagcagcgg gacgcggatt tccgcatccg
    17641 tcacctatgc gggcggcccg ctggggggcg accacgactt caccaaatac ggggcgacgg
    17701 ccgcctggtt ctacccgctg cccctggaca tggtcttcaa cattcggggc cggatcggct
    17761 atctccagcg aaacggagac accaagctcc ccatctacga gcgctatgtg ctgggcggca
    17821 tcagcaccct tcgggggctg cgcgacgtgg gccccaagga tgagaggacg ggcgacatca
    17881 tcggcggcac gacgatgctc tgcgggagcg tcgagatcat cttccccctg atccggaacg
    17941 ccggcatgaa gggggtcgtc ttttacgata cgggaaatac ctgggagagc ggctattcgt
    18001 tgagcgatct ccgccagacg gccggcgtgg ggatccgctg gtattccccc atcggccctc
    18061 ttcgcctgga atggggctac gtcctggaca agaaggatga tgaagatgcc agccgcttcg
    18121 aatttacgat cgggatgttc atgtaagggg cgggcgaagg gcgcggggga atcgactcct
    18181 tccgccttga accgtcagcc cttcgctcta aacaaaccgg tgaaaggaaa agggccggag
    18241 gcgaggggaa ccgattcctt aagccttgac cgttgaccct tcacccatca aaagggggta
    18301 ttggaagcat gaaaagattt ttgttgctct cgttgggagg catgctgtgt ctcgctctgc
    18361 tgatctgcat cccggaggtt caggcggcgg agaagaccgg tttcatcgac atgcgcgagg
    18421 tcatgctcaa ttccgaagcc ggtaaaaagg cgaccgagga attcaagaag atttacgaac
    18481 gcgacaaggc ctcgatccag gagaaggaaa cggagctgcg gaaactcaag gacgatctcg
    18541 aaaagcagcg gtccatcctc aaggaagagg cctaccggga aaaggaggcc gcgtatcaga
    18601 agaagttccg tgactaccag ctcctggtca aggacgcaaa cgaggacctc caggcccgcg
    18661 atcaggagct ctcccggacc ctgatccctg aaatcctgaa ggtcgtcggc gccatcgggg
    18721 aaaaggaaag ctacaccctc gtcctcgaca tcgggacggt accggtggcc tatttcaaca
    18781 aggaaaacga tctgaccaag cgggtgatcg aagcgttcaa caagacctac aaaccgaaga
    18841 cgccttagtg cggaaaggat tcctcaaccc ttgccaaccc tgaaagagat cgcagccttc
    18901 ctggacggtc gggtgatcgg cgatccggag gtcgtcatcg aacgggtctg cggcatcgac
    18961 gaggccggtc acggcgactt gaccttcgtg gccaacccga aataccggtc ccgcctggcg
    19021 acgaccggcg cctccgccgt tctcgtggcg tcgggcacgg aggccgccgg caagaacctg
    19081 gtctgggtcg cggatcccta cgtcgccttc ggccaggccc tcgggttact ccatccggag
    19141 gataaggcgg cggccggcat ccatccgcgg gccctggtgg agggagaggc attcgtttct
    19201 ccggaggcaa ccgtctatcc gggcgtttat gtgggtcaac gggcccggat cgaccgggga
    19261 gcggtcctct tccccggcgt ctttatcggc gcggacgccg tcgtcggcga ggacagcgtt
    19321 ctccatcccg gggtcacggt ataccgccgc tgtctcatcg gccggcgtgt cacgctgcac
    19381 gccggcgtcg tggtgggcgg cgacggattc gggtttgccc gaccgggccg ggaaaaccgg
    19441 aagattcccc aggtgggcat cgtgcagatc gacgacgacg tcgagatcgg cgccaatacg
    19501 accatcgacc ggggcaccct cggacggacc tggatccagc ggggggtgaa gatcgacaac
    19561 ctcgtccaga tcgcccacaa cgtggtcgtc ggcgaaaaca gcatcatcgt ctcgcaggcg
    19621 gggatctccg gcagcacgac ccttgggaag agcgtcattc tggggggaca ggccggcgtc
    19681 gtcggccaca tccgggtcgg ggatcacgtc atggccgccg cgcgcacggg cattcacaag
    19741 gacgtcccac cgtcccagat catctccggc gccccccacc ggccccaccg ggagtggctg
    19801 cggcaggagg cctgtctgtc gaaactcccg gagttgagac agacgcttaa aaccctgcag
    19861 cgccgggtgg ccgccctgga gcggcagaca gcaaaaaaat cactttgaag gaacgtgcca
    19921 tgaagattca tcccaccgcc gtcatctctc ccgaggcccg gcttgccgag tcggtggaag
    19981 tcggacccta cacgatcgtc ggaccggacg tcgagatcgg cgccagcacc gtcatcgggc
    20041 cgcacgtcgt catcgaaaaa tgcaccgaca tcggcgaggg gtgccacatc cgccagtttg
    20101 cctccatcgg cgccgacccc caggacctga aatatcaggg agagcccacg cgcgtcgtga
    20161 tcggcaattt taacacgatc cgcgagtgcg cgacgatcca ccgcgccacg acggccgaca
    20221 tcggcgtcac gatgatcggc gaccacaacc tcctgatggc ctacacccac gtcgcgcaca
    20281 actgcaagat cggcaaccac gtcatcatgt ccaacgccgt gaatctggcc gggcacatcc
    20341 acgtcgagga tttcgccatc atcggaggca tgaccggcat ccaccagttc acgcgcatcg
    20401 gcgcccacag tttcatcggg ggcgcctcgg ccgtgccccg ggacgtcccg ccgtacgtcc
    20461 tggtctcggg caacaccgcc aaaccgtacg gactgaacct ggtcgggctc aagcgccggg
    20521 gtttctccga ggacaccctt caggccctga agcacgcgta ccgcctggtc ttccgttcct
    20581 ccctgcggct cgcggtcgcc ctcgaccgag tcgagcaaga ggtgccggac cttccggaga
    20641 tccggcagtt tgtcgagttc atccggcatt ccgcccgggg catctgtcgg tagggacgca
    20701 tcatggccaa gatccgcatc ggcgtcgtgg gcatcgggca tctggggacg taccacctgc
    20761 agaaattccg gatgatggat gcctgcgtca ttacggccgt ttccgacagc gacggcgaaa
    20821 aggcggcaaa ggcggcggcg gaatacgggt gcgcggcttt cgaggactac cgcgacctca
    20881 tcccccatgt ggatgccgtc agcatcgcgg tcccgacagc cgcccacccc gaggtcgccg
    20941 gcgccttcct cgcggccgga atcgacgtct ttctggaaaa gcccctggcc cccacccttg
    21001 ccgaagccca atccctggtg gcgctggccg aggccaacgg caggaccttt caggccggct
    21061 tcatcgagcg cttcaacccc gccattgtcg ccctgaaggc gcttctcggg gtgcccctat
    21121 tcgtcgagtc gcatcgcctc caccccttct tcgagcgggg cacggacgtc gatgtcattc
    21181 tcgatctcat gatccacgat ctggatatcc tggcctggtt cgttccggcg ccgctcgcgc
    21241 acgtcgaggc cgtcggggtc cgggttctat cggagaacgt ggacatcgcc aacgcccgcc
    21301 tgaccttcga gaacggatgt gtcgcaaacg ttacggcaag ccgcgtcacc ggaaaaaccc
    21361 ttcagaagat gcgattcttc ggcctggaag gctatcacgc cgtggatttc gcccagcggg
    21421 agctggtttc cctcaagcgt gtcagacagg ccgacggcaa gatcgacatc gtcgacaacc
    21481 gggccgacgt cattccaaag gacccgctcg aggcggagat ccgcgccttc gtcgaggccg
    21541 tcctccggcg cacgccgcca ccggtcacgg ggcgggatgc cctggccgcc caggagatgg
    21601 ccggcctcat cggtgaacgg atccaggcgg tggaggcgcg gtgggcctga tgtccggaat
    21661 gcttttccag acccgtcgcc gccggcacgg ccggatcgcg tgccgcgaga gatcacccgg
    21721 ggaggtccgc cgatgtcggg caggcgcgta atgattgtcg cgggcgaggc ctcgggcgat
    21781 ctccacggcg cccgtctggt ccaggcgatg cgtgccctcg atcccgcgct tgcctttagc
    21841 ggaatcggag ggcggaacct gcgccaggcc ggtgtgcggt gtctggccga cgtggcggat
    21901 atgggcgtcg tgggcttgac cgaggtgacc cggaagctgg gcaccattct ccgggcgagg
    21961 cgcgacctgc gccggattct gtccgacgcg cccccagatc tcctgatcct catcgactac
    22021 cccgatttca acctccccct ggccaggatc gccaaggcgc gcggcgttcc cgtcctgtat
    22081 tacatcagcc cccagatctg ggcctggcga aaggggaggc tgaacgcaat ccggaagtgc
    22141 gtcgatcaca tggccgtgat tctcccgttc gagaaacccc tgtacgacca ggccggcgtc
    22201 cccgccacct tcgtcggcca ccccctgctt gatgccctgg gggacacccc cgccccgccg
    22261 gaggcccgcc ggatgttcca gctggcggaa ggcgtcacca ccgtcgcgct cctgccgggc
    22321 agccgtgagg gagaggcaag aaagctcctg ccggagatgc tcggggccgc ccggatcatc
    22381 gcccgggagc tgccgccggt ccagtttgtc ctgcccctgg ccgacagcct cgacccggcg
    22441 gtcgtggagg gcatcgtccg gcggcgggac ctcgcggtcc ggatcgtccg caaccggatc
    22501 tacgacgtcc tgcccgccgc cgacgtcgcc ctggtggcct cgggaacggc cacgctggag
    22561 acggccctca tgaacctccc catggtcgtg gtctacaagg tctcgcccct gtcctactgg
    22621 gtcggccggg cgttcatcca tgtgggccac atcagcctgg tcaacatcat cgccggtaag
    22681 acggtcgtgc cggagctcat ccagggcgag gcgcacgcga accggatggc cgtggaggtg
    22741 atcgacatcc tgagccgcaa gtcgcggaag ctccggatgc acgaagaatt gtcgcaaatc
    22801 cgcgcccgcc tgggcgagcc gggcgccgcc gcgaagacgg cggccctggc gatggacctt
    22861 ctgaacacgt gaccgacggc gcgcttcgaa acgcacccgg ccgccgcgga caaggacaac
    22921 cgatgaaacc acaacccaca ccgcctcgcc cccccgggaa ggaatccggc ggggacattt
    22981 acatccgcct gctgaagctc gtccggcccc atgcgccgaa gttcgttctc gccatgctct
    23041 gcatgctcgt cgtcggtgcg ctgacctcgg ccctggcctt tctcgtcaag ccggccatgg
    23101 acgacatctt cctcaaaaaa gacacccgca tgctcatgct catccccgtc gccgtcgtcc
    23161 tgatctacct gatcaagggc gcgtgccact acgcccagtc gatcatcatg aacttcatcg
    23221 gccagcgcgt gatcgccgat ctccgctacc ggctgtacga aaagatccag cgacagtccc
    23281 tggccttctt cacccagaac ccaacgggcg ttctgatctc gcgcatcacc aacgacgtca
    23341 atttcatgaa agaggccgtc tcggaagccg tcacgaacct gttcaaggat tccttcacca
    23401 tcgtctgcct cattttcgtg atcttttacc gcgactggaa gctggccatc atcgcgatgg
    23461 tcgtcttccc cctgacgatt taccccatcg ccaccttcgg gcggaagatg cggaaggtgg
    23521 ccacccgcac gcaggtcacc atgggcagcc tgacgagcct ccttcaggaa acgatctcgg
    23581 ggacccggat cgtcaaggcc ttcagcatgg aagaacacga aaacgagcgc ttcgccggtg
    23641 aaaacgagcg gttgttccgc ctgaccatga aatcggtcgc catctccgcc atttccagcc
    23701 ccttcatgga gtttttgggc ggcatcggca tcgccgtcat cgtcttctac ggcggctacc
    23761 aggtcatcca gggccactcc acgccgggaa cgttcttctc ctttttgacg gcgctcatca
    23821 tgctctacga accgatcaag cgcctgacca acgtcaacaa caagattcag cagggcatct
    23881 ccgccgcgat ccgtgtcttc ggaatcatcg acacgacgcc ggacatcctc gatgcgcccg
    23941 acgccgtgga actgcctcgg atcacgcagg gcatcgccct ggaggacgtc tcctttcgtt
    24001 acgaggatac gccggtgctg aaaaacatcg cgctccggat tccggccggc gaggtgctgg
    24061 ccttcgtcgg gatgagcggg ggcgggaaga cgaccctggt caacctgatc ccacggttct
    24121 acgacgtcac ctccggcagg atcctgatcg acgggcgcga catccgcacc gccacgatcg
    24181 cctcgctccg gaaacagatc ggcatggtga cccagcagac gatcctgttc aacgacaccg
    24241 tccgcaacaa catcgcgtac gggagcttcg aaaaaccgga agccgagatc atccgggccg
    24301 cccgggccgc caacgcccac gacttcatca cccgtctccc gcagggatac gacacccgga
    24361 tcggggagca ggggaccaag ctctccggcg gcgagcggca gcgcatctcg atcgcgcggg
    24421 ccatcctgaa ggacgccccc atcctgatcc tggacgaggc gacctcctca ctggacacgg
    24481 aagcggaaat tgaggtccag ggcgccctgg agaacctgat gcaggggcgg acaaccctgg
    24541 tcatcgccca ccgcctctcg accgtccgca atgccgaccg gatcgtcgtc ctcgtcgacg
    24601 gccggatcat cgaggagggg acccacgacg aactgctcgc ccggcagggg gagtacgcga
    24661 agctctacgc catgcagttc aaggaagaga acggcaacgg cgtgcgcaac cggaccccgt
    24721 ccccttccgg ttccgcggcg acgggggagg cgtgatgcat ccgcaggcgg cccttgaagg
    24781 gatctggtat cggcccgggg cgcaggcccg ccggcatccc ctcttctggc cgctctacgc
    24841 gctgtccctg ccctacgggg cggcgatcgg tctgcgcaac cgcctgtacg acggaggcgg
    24901 ccttccccgg acgaaactcc cctgccccgt catcagcgtg gggaacctca ccgtgggggg
    24961 aacggggaag accccgctgg tcatgctcct cgcggaactg atgcaggagg cgggctggcg
    25021 cccggccatc ctcagccggg ggttcggcgg ccgctcgtcc cggggggttc acgtcgtctc
    25081 ggacggccga cgcctgctcg ccacacccga aacggtgggg gacgagcccg tcctgatggc
    25141 ccgcaccctc ccgtcggttc cggtgctgac gggaccggac cgcgtccgga cgggcaaggc
    25201 ggcgctcgac cgcttcgggg cgaagctcct gatcctcgac gacgccttcc agcatcggcg
    25261 cctcgcccgc gatctggaca tcgtcctgac ggatctcacc ctgcccttcg gcaacggctg
    25321 cctcctcccg gcgggcccgc tgcgcgaacc cgtccaggcc ctcaagcggg cccggatcgt
    25381 gatccggacc ggatccgaag cggagatccg cacctccccc gcgtcggcgg acgccggcgg
    25441 tctgcgggga tttcaccgtc cccgtgagct catccgcggc gatggcgccg aacgacatcc
    25501 ccccgcgatg ctcgccggga agcgcctcct ggcgttcgcc ggcctcggcc ggcccgacgc
    25561 cttccggaag accctcgcgg cgctgggcgc cgaggtggcc gattttctcc ccttcccgga
    25621 tcatcacgcc taccgccgct cggacatcga gggcatccgt cagcgggcgg tcgcggcaaa
    25681 agtcgaattc atcgtcacga cggagaagga cgcggtccgt ctggccccat gcgccgactt
    25741 tctcggagag atcttccagc tccggatcgt catggacatc ctgcccgaac gggagacgct
    25801 ggcggcgagg gtgctcgaga ggatcgcccc atgagggacg caggacacgc cgccgtcttc
    25861 ctggaccggg acggcacgat caacgaggag gtgggctacc tggaccgtat cgacaggctc
    25921 cgcctgcttc ccggagcggc cgagggcatc cgcctgctca acgcctcggg cttcaaggtg
    25981 gtggtcgtga ccaaccagtc cggtgtggcg cggggcctgt tcacggaggc cttcgtcgtc
    26041 gaggtccatc gcacgctggc ggagctcctc gggcgccagg gggcccggat cgaccgtttc
    26101 gagtactgcc cgcaccaccc gaccgagggg gcgggtcggt accgccgggc gtgcccgtgc
    26161 cggaaacccg aaccggggat gctgctcaag gccgcggcgg cccttgccat cgatctcgac
    26221 cggtcttacg tcgtggggga cacgcccagg gacctggagg cggcccggcg cgccggtgcg
    26281 cgggggattc tcgtccgcac gggctacggc acggaacacc tgaaagccgc gtcagccgcc
    26341 gagacgaccg ccgcgcatgt cgccgacgat ctgctcgcgg ccgtccactg gatcctcacc
    26401 gaggcggacc ggcacggtcc ggatccccgc gaccggaggc ggccatgaac ctcctgatcg
    26461 tcaaattgag cgccatcggg gacgtgatcc acaccctgcc ctccctggcc gccctgcgcc
    26521 gtcacttccc cgcagcccac atcacctggg tcgtcgagga ggccgcgagc gacctgctcg
    26581 cggatcaccc ccacctcgac cgcgtcctcg tctcccgccg cagacgctgg cagagcgacc
    26641 tgaagcgggg gcgcgtccgg cagccgctgc gggcgatcgc cgcgtttctc ggggacctgc
    26701 gctcccgccc ctacgacctc gtcatcgact tccacggcct gttcaaaagc gcgctcctgg
    26761 tcggtctgag cggggggcgg aggaaactgg gctatgacag cctgcaggag ggcagcggcc
    26821 tgttctacac ggagaagatc ccggaggacc tgacgcgcca cgccgtggat cgttatctcg
    26881 actttgcgcg tcacctcggg atcgaacccg gaaaggcgga attcctgatc ccggacaccg
    26941 ccgaccagcg gggaagaatc gattcgctcc ttcagggcct cggcatcgcc ccgcgggagc
    27001 ggttcatcgc cctcagcccc gtcgctttct gggaaacgaa actatgggaa gaggcgcggt
    27061 tctcccgcct gggcgaccgt atccgccgcg aactcggcct gccggtcgtt ctgacggacg
    27121 gcgcatgcga ttacacggca cggatcgccc ggggcatgac ggaggccgcc gtcaacctgg
    27181 gcggccggac cacgctccgg gagctggccg ccctctaccg gcgggcggcc ctgctggtca
    27241 cgaccgactc cggacccatg cacctcgcgg ccgcggtggg ttgtcccgtg gtggccctgt
    27301 tcggccccac cgacccccgc cggaccggac cgtacggacc gggacacacc gtccttgaga
    27361 gtcccgtcga ctgccgaccc tgtttccgca agacttgcga gacccgccag tgcatgcggg
    27421 acatcacggt cgatgccgtg ttcgaggcgg tgcgggaaaa actgacggac cgccggcaga
    27481 aaatgcatgc agaaaaaacg ctcgaataag gagaacccca tgaccctcaa caaggacctg
    27541 ctcgacatcc tggcctgccc caagtgcaag ggagagatcc atttaaacga cgccggagac
    27601 gggctcatct gcgacgcctg ccgcctcagg tacgaaatcc gggacgacat tcccatcatg
    27661 ctcattgaag aggcccgatc cctggatgag ggaccatgat ctacgccaag ctgttcctga
    27721 cggccctctt ctgggggggc accttcgtcg ccgcccgcgt cgtcacgcaa gagttctctc
    27781 ccttcagtgc atccctgtgg cgcttcgtca tcgcttcggc ggtcctggcc gtcccggtcg
    27841 tcctccacga ggggggactg ccccgtctcc ggcggcgccg ggtcctgccg gtcgtcctcc
    27901 tcggcctgac gggcgtcctc gcctacaacg tctttttctt cctcggcctc aagaccatcc
    27961 cggccggacg agccgcgctg atcgtcgcca acaaccccgt cttcatcgcc ctgttttccg
    28021 ccctgctctt caaggaacgc ctctcgatcg ccaacgggat cggcatcggc gtgtgtctgg
    28081 ccggggccgt catcgtcatt tcccggggga atcccggcgg cctcctgtcg ggtgcaatcg
    28141 ggccgggcga gcttttcatc ctgggctgcg tcgcaagctg ggtgtcctac tccctcatcg
    28201 gcaaggtcgt tctgcgcgaa ctctcgccgc tggcggccgt ggcctggtcc tgcctgatcg
    28261 gcgcggcggc cctgctgccc ttcgcctggc aggaaggcct cgtccggcag acggctctcg
    28321 cctcgccggc ggcctgggcc ggtctgctct tcctgggctt gccgggaacg gccatcgggt
    28381 tcctgtggta ctacgaaggc atccgggcca tcggtccttc ccgcgccgcg gtgttcatca
    28441 atttcgtccc cgtgagcgcc gtcctgttcg gttggctgat cctctccgaa acgctgacgc
    28501 gcagcctctt gacgggcggc gccctcgtcg tcgtcggcgt ctttttgacc aaccgggcgg
    28561 gcgggcggaa aacccttgaa acatagcgaa aatcatgcta cggctcccca tgcgtttctg
    28621 gtgtgccgcc ccggcggccc tgggtcgtct ccgcggggga tgggcgcctg acgtgcgtcg
    28681 ccggggaaaa aggggctcat cttgaatccg ttcacgatcg cgacctacaa cgtcaattcc
    28741 atccgttccc gactgcacat cctcctccct tggctggaaa cataccggcc cgacgttttc
    28801 tgcatgcagg aaaccaaggt cgacgacggc cggtttcccg cggacgcctt cacgaacctc
    28861 ggctatcact gcatcttccg gggcgcaaaa cagtacaacg gggtcgccat cgcctccctc
    28921 gccgcgccgg aggcggtccg ctacggattt tccgacggcg gctcccccga tgacgaccgg
    28981 cttgtgtgcg gcgttttcga cggcgtggcg gtcgtcaacg cctacgtgcc ccaggggcgg
    29041 gaccgcgaga gcgaacactt cgcctacaag ctcgcgtggt ttaagcgtct gaaaacgttc
    29101 ctggccgccc attactcccc cgcccaaccg ctggtctgct gcggcgacct gaactgcgcc
    29161 cgggaggcga tcgacgtcca cgaccccaag cgcttgctgg gacacgtctg tttcacgcct
    29221 gaggtttggg acgccttcga cgcgctgacg gcctgggggc tggaggacct tctccggcgt
    29281 catcacccgg gtgaagcgaa ccactacacc ttcttcgatt accgggtccc ccggtccctc
    29341 gaacggggcc tgggatggcg gatcgaccac ctgctcgtca ccgcgccgct ggcggaacgg
    29401 tccctgggct gccggatcga tctggaaccg cgccgggcgg tgaaaccctc cgatcatacg
    29461 atcctgatcg gcgaattccg gcgctgaggc gcgtgaggtc atggacgaaa tccaggaaac
    29521 gctgcgatgg aatgaagacg tggcccggaa actctcggga atcgaacggg agatcgccga
    29581 tagtcccgat cctgcgggtc ttttcgagca tctcgtgctg agcatggaga ccgccttcgg
    29641 gatccccttc gtctggctct cgctggtccg aacccctcag gccgacgccc tccggggcac
    29701 cctcgccgcc tccggcctgc tcggcgaccg gctgaacctg atcgacggcg acgccttcgc
    29761 ggcccttctg gacggccgga gcgaaccgct cctggtcaac ggtgatctcc gctccttcta
    29821 ccgtctgctc ccgcccatga aaaagttcct cttccggtcg ctggccctgg tccccttcac
    29881 cctggacgga acggtcgtcg gcagcctcaa tcacggtgac ccctctccgc agcgctatca
    29941 tccggacctg gcgacggatc ttctcgcgca gctcggcgcc cgcgtctccg cccgcctcgg
    30001 cgcgctgtcg tcaaaggccg ttccgctgcc cgcctcgccc tgacagggtg ccccggttcc
    30061 aagcctgcct ccggaaagcg ttgcccggca tccgggaaac ggtcccttcc gggtttcatc
    30121 ccttgacaaa cgttccacta atccgtacat tcagcaatgc ctgattgcgc tgatcgtgta
    30181 tgattcgcgg ggcatcgaac gggagggggt ggggttatgg tcaaggccga tccgaaggcg
    30241 tcctgctgcg cgcccggaaa ccggacgccg agcggttgcc acatcgaatc gctggtcagc
    30301 gtcgacgagc gcggacagat ggtgctcccc aaggacctgc gcgacaaggc cgggatcaag
    30361 gccggcgaca agctggccct cgtcagcctg aaaaaggacg gggagatctg ctgtctggcg
    30421 ttgatcaagg tcgacacgct ggccgaccag gtcaaggaat ttctcggacc ggtgatgcgc
    30481 gatgcggtta ccggctgatc gtggcggcgg atctccgctt cgggagcgga tcccatgaaa
    30541 tggcctcgga acaattcagc gaatgaggcg gtcctcttgc ccatggcatc gccgcaggcc
    30601 ctgtccgccg cggatatcgc acttccgatg cggccgcccg gactggatca gccgttcgtg
    30661 aacggcgctg cggaaggccc ggcgggaaag attccccggg tttcgtcggt gctcacttgg
    30721 cgggacagct ggggttcgat caaggcccgc tggggcgtcg gccggatgga ttatgccgtc
    30781 gatccgggtc tatacgcgct cgggtcgccc gatgcggggt cccccgtgct cgtcacggcc
    30841 aactacaaga tgagcttcga ccgcctccgg gaggcgctga cggggcggaa tgcctggatc
    30901 ctcgtcctca acacggaggg aatcaacgtc tggtgcgccg ccggcaaggg gacgttcgga
    30961 acgggggaac tcgtccgctg catcgtcgcc agcggcctga aagattgcgt cagacaccgg
    31021 gagctgattc tgccgcagtt gggggccccc ggcgtttccg cccattgggt ccggaaaatg
    31081 tcggggttta cggtccatta cggcccgata cgcgcgcggg acattcccgc ctatctcgac
    31141 ggcggcctga gggcgacacc gaagatgcga cggaaaacct tttccctgcc ggagcgcgcc
    31201 gtcctcattc ccatagagct ggttgccgcg ctaaagcctg tggccttgct cgtcccgatc
    31261 ctggtcctga tcagcgggct cggcggccgg ggcgcgttcg tccagaacct gatccatgac
    31321 ggtctgtttt cgttgacggc cttgctgctg gcgatcctgt ccggggcggt cttgagcccg
    31381 ctcctcctgc cctggctccc cggacgcgcc ttcgcaacga aaggcctggc cgtcgggctt
    31441 gcggccgcga tcgccctgct ctcttcctgg gggctgaatc tcaaaagcgg gcgggacctg
    31501 ctgctcggag gcgcctggct cttgatgatg ccggggattt ccgcctttct ggcgatgaac
    31561 ttcaccgggg cctccaccta cacgtcgctc tccggcgtga ggcgggaaat gcgctgggcg
    31621 cttcccgtcg aaatcggggc cggtctttcc ggattggccg tactcctggt ctcccgcttt
    31681 ttttaagagg atgaggcgaa gacgatgatc tacctgaaga acgtggtcac ccttgaagtg
    31741 gaccgggaca aatgcaccgg ctgcgggatg tgcctggaag tctgccccca cgcggtcctg
    31801 gcgatggagg atcggcgcat ccgcatccgg aatcgcgatg cctgcatgga atgcggcgcc
    31861 tgcgcccgca attgcccggt cggcgccgtg tccgtcgagg ccggtgtcgg gtgtaccgcg
    31921 gccgtgatca acggactgct gaaccgacaa gccccgtcct gcggttacag cctcgatccg
    31981 aagcgtccat ctgcgggaaa agtcggaggc tgctgctgaa gttcatcgtt gcgaccgcac
    32041 ctatccgtca tcccgaagca ggcacgtgcc tacgaataca tcttctcgat ggcgtctgcg
    32101 tactttacgg cgatcacccg ccgtttcacc ttgagggtcg gcgtcagctc gccggtttcc
    32161 tgcaaccatt cccgatcgag cagggtgaac ttcttgacct gctcgacctg accgaagtcc
    32221 ttcgtaaacg ccttgatctg ctgttcgaac agggcctgaa cctttgcatg cttcaccagc
    32281 tcggaccggt cggcgaaggt gacaccgttc tccttcgccc agggctcaag aacctcgaac
    32341 gccgggacga tcagcgccga aatgaatttc ttcccctccc cgacgacgga aagctgattg
    32401 atgtaggggg agaacatcag gctgttctcg atattctgcg gggagatatt cttgccgccc
    32461 gaggtgatga tgagctcctt ggtcctgccg gtgatcctca gattcccctc ggcatcgaac
    32521 tcccccaaat cgccggtttt caggaatccg tccggcgtga acgccccttt ggtcgccgcc
    32581 tcgttcttgt agtagccgac catcacctgg gggcccttca cgaggatctc gccctcttcg
    32641 ctgatcttca cctcggtgtc gatcacgggc acgccaatgg tccccggctg gatatggccg
    32701 acgtagttgc acgtcaccat cggagaggtt tcgctcagtc cgtaggcgtc gcagcaggga
    32761 atgtccattc ctaggaagaa ctccagaacc ttgcggggca tgggcgcccc gccgaggccg
    32821 atgaaataga tctgatcgag gcccagcacg ctcttcagct tcgagaagat cagtttgttc
    32881 gccagggcgt actcgatcgc aaacaggccg gtccgcgcct tccccttgca aacgaaaggc
    32941 acgtttcggc ggccgacgtc gagcgcccag gcgaaaagct tctgcttgag gggcggggcc
    33001 tgggccacct tcgcggtgat cgcctcgtac atcttctcca gtacccgcgg caccgaggcc
    33061 atgatggacg gatggattgt ctgcaggtct tcaagcagcg tttcgatgct cttgacgaaa
    33121 tggatcttgt tggcgcagta gacttggcag tagtacacgg cggtgcgctc aacggcatgc
    33181 gagaggggaa ggaacgacgg ggccttgtgg aagcggttca ccatctccgg gaaataggcg
    33241 ttgaactggt ggacgttgga gatgatattg tggtgtgata gcatgacgcc cttgggatcg
    33301 ccggtggtcc ccgacgtata gatgaacgtg cacagatcct cgggcttgat cgccgccagc
    33361 cgccggtcga agagcgccgc gtcggcctgc gcgcctccct gccggatggc ctccgggagg
    33421 gtcaccaccc cgtccaccgg ctgttccaga tcattgaaga ggacgatctc catgagcatc
    33481 ggcagccggt cccgcacggc cacgaccttc tccatgtggg cgtgggtgcc cgcaaagcag
    33541 atccgcgaat cggcgttgtc gaggatgtaa cgtacctctt ccgcggtgtt cgtcgcgtag
    33601 atggggatat tcaccgcccc gatcgagaga atcgccagat cggcgaccca ccactcgtaa
    33661 cggttcgggg aaaagaccgc gaccttgtcg ccgtgttgaa tccctttgcc catcaggtag
    33721 taggccaggt tgtggaccat ctcgttcatc cggttccacg aaacgtcgaa ccattgaccc
    33781 gcgtcgttgc gataggccac gcacgccttg tcgccgtgtt cacgggcgcg attctgaaaa
    33841 accgccgcca tcgaggtttc ttctaacttt ttcacaggtc acccctttct tgaaaacctt
    33901 gcccccgccg gccgcgggac atgaaggtcg atcccggtgg aggctcagtc caccatcatg
    33961 aggcagtact cgttgttcgt ctgctccctc acccgcaggg tttcgatgaa aaatccgttg
    34021 ttgcgggccg tctggaaaac gtcaatcccg caggcctcca ttgcgggacg ggcgcggtcg
    34081 gcatgcagac aggaaacgtt ggagagtttc ccgcatttct tgcacaggat gcagggaccg
    34141 gccagaaaaa caaaggcctt gtagtatccg tccttgaaga gctccccttc cagactcacg
    34201 agcatgtcca tgaaggcccg gtaccgcttg ccccgttcag gcgtcgccgg tgcttcgatg
    34261 tggaagagga tcgcccgccg gtaggaatcg agaagcgccc gcgtctggtc atcagtgggc
    34321 gtatggggag ggcagcaata tcccatgccg tagccggggc agccgaactg gcacttccat
    34381 ctcacccagg gggcggtcgc gaccatgccc ggatgggtga tcctgaaatg cgccgcgccg
    34441 ttctcgagcg ccttccggca gtacggcgcc agatcctcat tcatcttcaa cgcatcttca
    34501 atcgcatccg cattcatggt tcctccgtat cgagcgttag atccgttcct tgaactcctt
    34561 gcctaaaagc ccttcgtgct tgaacaaaag aacgagaatg atgatgagga aaggcaccgt
    34621 attcttgaat tcgagggaaa tgtaaccgcc gcagaggttt tcgatgatcc cgatcaggag
    34681 cccgccgatg atggcgccgg gcagactgtt caatccgccc aggatcgcgc cggcgaagcc
    34741 tttcaggaag ggctccagca tgaagaaggg atcgagaatc agggccggtg ccaggaagag
    34801 cccggccaga acccccagaa gggcggccaa cccccaggag gtcgcgagga tggtccgggt
    34861 cggaattccc agggtctgtg cggcgggcag attctccgac gtggccctca tggcgaggcc
    34921 caaacgggtc ttctggatca ggagatacag caggatgctg cccaggatgc cgacgatcag
    34981 tgtgccgagg ctcagctcgt tcaggtagat cggccccacg ttccaggtct tcgtgtcgga
    35041 caggggaaag gcgagcattt tggagtccgt tccccacacg taaatcatgg cgccttgaaa
    35101 aatcatgctc aatcccacgg tgagggcaat ctgccccagc agggtcgcgt cgcgtcgctg
    35161 ggccggaacg atgatggaaa agtaaaagac gaccgcaagc agggcgccga aaagcaggct
    35221 gagcgggagc gccagataaa aggggaagcg ccaggacagg aggctgaatg caaaaaaggt
    35281 ccccatcgtg gccatgtctc cgtgggcgaa attcaggatt ctcgtggagc ggtagatcag
    35341 caccacgccc agggccacca gcgcgtagag gctgccggcg gacaggccgg aaatcatgta
    35401 ttgggtgaag ttgaccatgg aggactcctc ctcgtcgcta gcggaacggc cagttgcggt
    35461 aataaagacg gatcttgaac caccgtcccg cgagcccgag gggttcgaag atcatgatca
    35521 agacgaccgt caccccgaag atcaccggca cgaattcccg gtagccgctg aagatctggg
    35581 gaatgaggat gacgaaggcg gctccgaaga tcgccccctc caccgaggcc aatccgccga
    35641 tgaccaccgc cacgaagatg atcagggatt cggagaaatt gaacatctgg ggttcgatga
    35701 accccatgaa catcccgaac atggcgccgt ggatccccgt gtagaaggag ctgatggcga
    35761 aggacaggag cttgtagttc gttaggttga cgccgaggac ctccgccgcg atgtcgttgt
    35821 cgcggatggc gacgaaggcc cgaccgaggt aggacgagac gaggttgtac gtgaccaggg
    35881 taaacgtcag ccccagcaga agggcaaagt agtacatcga cacctcccgg gaaaatccga
    35941 agagggcggg aaatttagga acgacaagcc ccatgcgccc ccccgagagg acctcgaaac
    36001 tggtgaaggt ctggtacacg gcaatcccga accccagggt gacgatggcc agatacggcc
    36061 ccttcaggcg gagggagggg aaaccgacga gaaccccgaa gaaggccgcc atgagccccg
    36121 cggccaggag cgcaagaccg acgggaatgc ccagctttgc caggtggccg aaggtgaagg
    36181 cgccgatggc gaggaagccc gcctgcccca gcgagatctg cccggtgtat ccgatcagga
    36241 ggttctgccc catgatgccg acggcgacga tgaagatcag ggtgcccaga tagacccagt
    36301 gcgcgctgct gaaccagggg agcagcacga ggactagcaa ggtcataccg atccagcaac
    36361 ccgtccaggg cttttcaatc agtttgagat cgtctctgta gctttccacc aagttcattg
    36421 tcgtttccgt ctcgctttcg ctccaccgct ttacgcttcg ccacgataca tctcgtcgat
    36481 caggtccttg tagagctttt ccacgttccg tcgcttcacc ttctgcgtcg gggtgacgtc
    36541 cccgtcctcg gcgtagaacc gccgggggat cagggcgaac ttcttgatcg tctccactcg
    36601 cgccaaggtc tcgttcacct ccttgacctc tccgtccagc agcttgacga tcgccgggtt
    36661 ctgcgtgagg tcttcgaagg tggcgaaggg gatccgattg tcctgggcga acttgaccac
    36721 gttgtcctcg tcgatcagga tcagggccgt gagataatgg cgaccgtcgc cgatgatcac
    36781 ggcgtcctgg atataggtgc tgaacttcag cttattttcg atgaactccg gcgtgatgtt
    36841 cttcccgccg gaagtgatca ggatgtcctt catccgcccg acgatcttga gccagccgtc
    36901 gtcgatggcg ccgagatcgc ccgtgtggag ccagccgtcc cgcagagtct cttccgtgag
    36961 ttcgggattc ttgaagtagc cctgaaagac cccgggaccc cgggctaaga tctccccttc
    37021 cgtcgagatt cgcatctcga ggcccgggag ggcctccccc acatagccgt accgcggctc
    37081 cttgtccagg cgctgcaggg cgataatgcc cgtcgattcc gtctggccgt agccgtcccg
    37141 gagcgggatg cccaggatat tgaaaaactc gaaaagctcc ttcgacgccg cggcggcgga
    37201 gcaaagcgcc cagcggattc gctcgaaccc caactgccgc ttgaggtggt agagcacgcc
    37261 ccagtaggca agcgcgtaga ccgcgcgcca gacgaggagc gacccggcac cggcctgccg
    37321 ggcctgctgg tagcgccgcc ctacctgtac gcatcgcctg tagaggaacc gcttgagcgg
    37381 ggttgagtcg tctatcttga tgtggatgtt ggaggcaaac ttctcccaga tccgggggac
    37441 actgccgaag atcgttggag agatttcccg gaggttggcc gggagcgtct ccagactttc
    37501 cacgaagttg acggtgacgc ccgtgaagac cggcatgaag accgaaacga ggttttcgta
    37561 aatgtgggcg agcggcaggt aggaaacgac ttcgtctttt tcgctcaccg gaatgatctg
    37621 gaggaaggcc tgggtcacca tcgagatgtt ccggtgggag aggagcgcgc ccttcggacg
    37681 acccgtcgtt cccgacgtgt agatcaccat cgccgtctgt tcgacgtcga tcgcccgtag
    37741 actgtctgtc acggcgaccg gatgatccgc caggtgcgcc tccgcccggc ggatgaactc
    37801 gcgtaagaaa acgacccggg gatgggtgta gccccacagg cccttctcgt cccagacaac
    37861 gatccgctgg aggcggaggt tcggcaggat atcgaggatc ttgtcgagtt gctcctcgtt
    37921 ttccgcgaag agcagctgcg cctccgaatg gtcgatgata taccggattt catcgggaga
    37981 ggacgtggga tagatcccgc atgtccgcat tccggcggtc ataatggcca tgtggcagat
    38041 cagccattcc ggacggttgt cccccaggat gcagaccgtc tgcccccggg gcagttcgag
    38101 cgacaggatg gcggcggcca cctgccggac gttctccccg tactggcccc aggtgacctt
    38161 gttccagatc ccgtactgct tgtgccggag tgccacgcgg gtacggtgct tttccacctg
    38221 ttcaaaaaag gcttggggaa cggccatcgc attcagcggc attaccatct cctccgcttc
    38281 gtgtagaatt tccagccctt ctcggaagcg ttcccctgct ccaggcccag atacagctcc
    38341 ttgacgtcgt cgttctcctt gagttcctgc gaggtcccct tcaggacgat gcgacccgcc
    38401 tccatgatgt agccgtagtc ggcgatgtcc agggccatcc gggcattctg ttcgaccagg
    38461 aggatcgtca tgtcccgctc gctgaccttg cgcagggtgt catacacgct gcgggccatg
    38521 atgggggaca gccccaggga gggttcgtcc agcatgagga gtttcggccg gcggaggatg
    38581 gcccggccga tggccagcat ctgctgctcg ccgcctgaaa gcgtttccgc ctgctgcccg
    38641 ccccgttctt tcaggatggg gaagaggtcg tagatgaagt tcaggtcgtt ccggaccgcc
    38701 ttgtcccgac gcccccagca accgagatcg agattttcct cgaccgtcag ctcacggaaa
    38761 agaccgcgat cctccggcac gtagacgatg cccagagagg cgatcttctc gggcgtgaga
    38821 ccgctgatcc gcctgccgca gaactcgatg gtccccttct tgggctggtc cttcaacagg
    38881 cccgcaatcg tcttcaggag cgtggtcttc cccgccccgt tcggaccgag aacggtgaag
    38941 atctcgtttt ccctcacggt cagggagatc ccccgcaggg cgtagatccg gtcgaaatag
    39001 agggtttcga tgcttgcgat attcaaaaaa ctcatgaggt ctcaccgaca taggcgcgaa
    39061 tcacctcagg gtttctctgc acctccgcgg gaggaccctc cgccaatttc tctccgaagt
    39121 tcaagaccac catgcggtcg gccagtcgag acacgatcct caggtcatgt tcgatgagca
    39181 gaacggcaaa accgaacctg ccccgcatct ccgtcaccag atacgccacg cgattttttt
    39241 cctccacggt caaccccgcg accggttcgt ccaggaggag gagtttcggc tccatcacca
    39301 tggcgcggcc cagttccacg cgcttctgca ccccataggg gcagtcgatg acccgggatt
    39361 cccggtaggc cgcgagatcg agaaaatcaa tgatttcttc caccttctcc cgatggatca
    39421 actcctcctt ccggatacgc aggaaggcgt ggaggaggct tctcctgaac ttcatgtgcc
    39481 ggcccagcat gaggttgtcg agcaccgaca tgttgttgaa cagccggatg ttctgaaagg
    39541 tccggccgat gccccgctgg gcgattctgt cgggggagag cccgatcagg ctctccccgg
    39601 caaacagaac ctctcccccg gtgggacgat aaacgccgga cacgcagttg aagagggtgg
    39661 tcttccccgc cccgttcgga ccgatgatcg aaaggatttc cttctcctcc atttcgaaat
    39721 cgatcgccga cagggccttg atgccgccga actgcacggt caggttgttg accttcaata
    39781 ggctcatggt cttcgaagaa ccttaaaaca aggggttgaa aatcataaac tcgctgatcg
    39841 gcacgtgctt gccgcccttc gcctcgcaga tccggatggc gttcagcccc attttctgct
    39901 tggccgagaa ggtgatgggg gctcccattc cctcgcactt gaaattcttg atgccctcca
    39961 tggccttcac gaagctctcg cgcgtcaggt tcttccctgc cttccgcaac ccttcggcga
    40021 tcagcatcat cgtcgtggcg ccggtcacgc cgaggaactc ccggcccttc agcgccggct
    40081 cgtacttgag gaggatgtcg acgaccctgt ccgcctgcgg atcgacgccc ggcagggaca
    40141 cgttgcccga gacggcgggg taagcgccct cccacagctc gccggcgatc ttgtacatca
    40201 ggggatcgcc caggggattg gcggtgaaaa ttttgggctt gtagccgatc ttcgccatct
    40261 ccttgatgat cagcgccgcc tgcgtcggtg ccccgtagat gaccacggcg tccgcgccga
    40321 gttccttcag cttcagggcg tggcccccca ccgcccggtc cgtcacttca aacgtgacga
    40381 agccggcgaa cttccccttg ccgccgagcg ccttgatcgc cttctgaatg ccgatttcag
    40441 ccgactttcc ccagtcgtcg ttctggccga aaagcgcgat cgtcttgatt cccatcttgg
    40501 tgacggcaaa tttggcgacc gcctcggact cgtcgatgta gtccgggtag gcgataaaga
    40561 tgttcctccg ctgcgcggcc ggcgtcccca gccagatgcg ggggttggca tggacgtgga
    40621 cgaccggcac cttgctcgtg aggaggaaat ccttgctcgc attggccgtc gccgaaccga
    40681 tcacgcaacc gtacgcgagc acgtcgtctt tcatctccgt caggttggcc agggcccggc
    40741 tcgggttgta gccgtcgtcc ttgaccacga acttgatctt gcggccgtga atccccccct
    40801 gatcgttgat gtgcttcgcc caggcggaat ttccgagcgc catggcgctc cacagcgccg
    40861 ccggtccgga catggggcag gtcatgccga tggtgacctc gtctttcgtc acgcccgggg
    40921 taccggcagc caggacggtc gccggcaacg aaaaaccgat gatgatcaac agaatccaaa
    40981 acaagcccct cctcatatgc cacctccttt tttaaaaagc accgtgtcca tcaccgagga
    41041 aagttcaggc gctttccccc ccctgtctga tcccttccgt ggcttcacct ttttccggat
    41101 ccaccgtttc aatattccca cagcactccc agtgcctgac ccagcacctg acgggttccc
    41161 ccgccgctga gcaggaccat ggcatcccgg agatagcgct gggcgtcgta ttccatggcg
    41221 taaccgtagc cgccgaaaat ctggacgccg tgcagggacg tcttgcgcgc cgcctccgcc
    41281 gcggcaaggc gcgcctggat gatctccggc aggcattccc gcccccggtc gatcagcgac
    41341 gccgcctggc aggtgagcag ttcggccacc cgcgcctgca gcgccatctc caccagcatc
    41401 tcccggatcg ccccgaactg gatgatcggc cggccgaact gcacgcgctc ccgggcatac
    41461 tggagtgcgt aagcgtaggc gccccgggcg atgcccatgc cgcaggccgc cacggccaga
    41521 tgctcggcgc tcatgacccc ggccagtttc ttgcgctccg tcggggcatc ggcgtctccc
    41581 cccagccttt cttccgcgcc aacggcaacg gcgtccaggc cgaccttgca caggctcatg
    41641 ccgtgaaatc cgaccgtctc cagcgcgcgg atcctgatcc cgcgggtgcc cgcggcgacg
    41701 atgaagaagg cgggggtccc gtcaccgtcc tccagggcag cccggacgag gatcagggcc
    41761 gcccggtcgg ccagacgcac gaacgtcttc gccccgttca ggaccactcg ctccccttcc
    41821 cgccgggccg tcgtccgggt gccgctctgg tcatagccct gatcgggctc gagcagcgcg
    41881 agggtcacga gcgacgaccc gtcgatgacg ccgggaagat agcgctcccg ctgcgcctcc
    41941 gttcccaggt cggcgagcac ctttccgccg cagaggctgt gggtggcgaa ggttcccgcc
    42001 aggggcggat agatgccggc cagctcggcc gtaacgatca ccgcgcccaa ggtgttgggg
    42061 ccgccccccc cgtccgcttc gggaatcgtc agggcgcaca ggccgatgtc cgacaccttg
    42121 cggaagagtt ccgcgggaaa ggccccctcc tcgtccatgc gcttgatctg ctcgcggtca
    42181 cacgccttcg ccacgaactt ccggatggta tcccggatga acccctgttc ttccgtcagt
    42241 gcgaaatcca tttttcaatc cttttctttt tccatgccgg gatcgggatg ggagacgccc
    42301 cccctcccgc ctccgttccg ttaccgggga tccgccttac accttgcggt ccatcccctt
    42361 ccagaacatt tcccggatct tcttcttgtc cagctttccg aggttcgtca ggggaatctc
    42421 atcccagaac ttgacgctct tcggggccaa aaggcttccc ttgcgctcct tgacgaactg
    42481 gacgagctcc ttttcgctcg ctgtcttgcc cgcatgcaac gacaccaccg ccgtcacttc
    42541 ctcgccccac ttctcgtggg gcacgcccac cacggccacg tttttcaccg cggggtgttc
    42601 atggaggaca tcctcgattt cccgggggaa gatgttgaat ccgccgctga tgatcatgtc
    42661 cttggaacgg tccacgatgt agaggtagcc ctcctcgtcg aactttccca cgtccccggt
    42721 ccacaaccag ccgtcccgga tcgtctccgc cgtctgttcc ggattcttga ggtagcccgt
    42781 catcatgttg atcgtccggc agacgatatc ccccgcttcg ccgcggggca gctccttgcc
    42841 gttctcgtcc acgatgcgga cgtcggccgt catgaccgga cgcccgcagg acgtgtagat
    42901 tctcatgcga cgttccggat cggcgatgac gtgctcctga cgggacaggg cgctgatcat
    42961 catgggggac tccgtctgtc cgaagagctg ggaaaaaatc ggccccagcg tgtccaccgc
    43021 ctgtttcagg cgcgccggag cgatggcgga agcgccgtag atgatgttgc gcaggctggc
    43081 gaggtcatac ttcttgaggt caggataatc caggaggaca tagatcatcg tgggcacgat
    43141 gaaggtcgtc gtgaccttga ccttctccac ccgctccaga aagagcttcg ggtcgaaatg
    43201 atcctggatc tcgcaggtcc cgcgtttcag caggatcggc aggatcaggc atcctcccgc
    43261 atgggtgagc ggcgtggcga aggcgaacac ctcgtcccaa tccagcccca gatggagcat
    43321 ctcgctgatg aacgtgccca cccaggtgtt gtggtccatc atcacgccct tgggaaggcc
    43381 ggtggtgccg cctgtgtagt agatcgcgac caggtccgtg cattcaagcg cgacccgcgg
    43441 cgcttccggc ggattcgccg cgatgaggga ctgcaggtgc gcgtgcccgg acgtcacggt
    43501 cgccggatcc tgggccacgc agatgaactg cttgaccgtc tcgagcgagg ggatcatctc
    43561 ctgaacccgg ccggccatcc gttcgtggta gatcagggcc ttgcagtcgg actgattcat
    43621 catgtagatg tgatccttgg aactgagcag gaccgccagg ggaacgcgaa ccaaccccag
    43681 ctttcccaac gcatactcgc agaagatgta ttccggacag ttggccatca ggaacgcgac
    43741 ccgatcgccc ttcttgagcc ccagcttgtc gagggcatgg gccagggaat aggcgttctt
    43801 ctgcagttcg tcgtaggtgt acgtctcgtc gttgaaaaga atggcggtgc gggaacgaaa
    43861 agccgccacc gaacggtcat acagatccgc gcaggtgatg ccgtgaaaca gtgttgccat
    43921 gacgtttttc cctttcttgc cggccccggg ccggcggcct gttcccaaaa caggcggccc
    43981 ccggcgtgcg acgattccgt tttttacgtg tagaatttct tgccgtagag tttctcgctc
    44041 aatccgatca ggtcgcccca tttcatcgga ccgcccttgt cggccgggaa accgaccccc
    44101 cagatcgcgc cgatgtcgat cgaccgcgga tcgctgacga ccttcttccg gagaagctcg
    44161 tgacccgtct ggacgaaggg ggtgaagagc atgtcctgca gctcggccac ggaatactcc
    44221 ttctgcctgt tgtccggatt gatcaagggc aggacctcgg gatcgggcgc gccgtcctgc
    44281 gcatagaatc cctttccgtt cttgttgcgc ccgtaacgcc ccgccttgta gaccctctcc
    44341 agggccgggg gcggcaccag ccccagggac ttcgtcagga cgagatagga aacgtcgatc
    44401 ccgacctcgt cggtcaggcg gatgggaccc acgggcagac cgaacttcat catggccgcg
    44461 tcgatcctgt ccatggccac gccttcggcg aggagatcga aggccttgat gaaatacggg
    44521 aagagcatgg cgttgaccac gaacccgggg ttgtcgttgc agacgaccgg gcgcttgccg
    44581 agggcggcgc agacggccag catgttgttc gtcgtggcgt cgctcgtggc ctggccccgg
    44641 atgatctcca gaagctgcat catccagacg ggggagaaga aatgggctcc cacgaaccgc
    44701 tccgggttcg agacgctctt cgccagttta tccacgggca ggctcgatgt gttcgtcgcc
    44761 aggagacagt cgtccgccac ggcctggcac agctcccggt agacctgatc cttgacggcg
    44821 ggatcctcga aaacggcctc gatgacgcaa tcgacgtcct tgaagtcgtg cgtccattcc
    44881 gccaccggct tgacccgggc gatcaggctg tcgacgggtt ccttcaggcg gttcttggcc
    44941 gccatgcctt ccagaatctt ccggaggaag cccatgcccg gttccagggc cccggagaca
    45001 tccttgacga cgatcggcag ccgggtccgg gtcaggatgt tgatgacgat cccgcgcccc
    45061 atcgtgccga agccgaggac gccgaatttc cggatgggct tgggctcgaa tcccctggga
    45121 atcatggcct gcggcttgtc ggacagggtc ttcaggaaaa aggtgttgat cgtccccttg
    45181 cattccggcg ccttgagcgc ctcgatgaaa cagtccgttt cgatcttgag gccctgttcg
    45241 atcggcacct tgagcccctc gctcatcgcc ttgagcgcca gcatcggcgc cggaatcgcc
    45301 cggccccgcg tcgccttgag cacgttcttt ttcgccgcct cgagcgtcgc gtcgagtgcg
    45361 gacagatccg gcttctcccg cttcaatccg gccgtcccgc cgaggatctc cgcgaggagt
    45421 gctttcgcct tcgccagcag gtcttctccc tccgggatca cccggtcgac catgccgacc
    45481 tccagggcct tggccgcggg cagaacggcg ccgcggggga taaaatccag ggccggatag
    45541 ccgatgagcc gggggagacg ctgggtgccg ccggcgccgg gaatgacgcc cacgttgcac
    45601 tcgggcaggc cgatcgtcgt ccgtttgccg tcggtcgcga tcctcgccgt catggtcagg
    45661 gcgaattcat accccccgcc catgcagggg ccatgaatgg cggccacggt cggtatgccg
    45721 aggttttcca atcggatgaa gagcccgttg aacgcatcgc accccttccg gaagtccgcc
    45781 agatccttct ccgcccccag catgttcagg ttcgcgccgg catgaaaatt cgtgcttttt
    45841 ccggagatga agatcacgcc ccggtaagcc accttctgac gctccagggt ttcgagcaga
    45901 acggcgaaat ccgcgatggc ctcctccgtc caggtgttca tcgcctcgcc gggcacgtcg
    45961 aaccggacca cggcgatgcc gtcctcgttg ctgcagttaa aaatccgtcc catgttccct
    46021 ctccttccgt caacgcatga tcggtccggc cgcggcctac gtcatccccc ggttcggccg
    46081 accctcaaag tccaaaggtc ttgcagatga tgttcttctg gatttgcgac gtccctccac
    46141 cgatggagaa gatgaggatc tcccggaggt ttctctgcat ctcgtaatcc atggaggatg
    46201 cgctctcgcc caggacgttc attcctttca ggatgatctg cttggccgtc tcggcgcaga
    46261 agtacttggt catggaggtt tcccggaccg gattcaaccc ctgcgcctcc ttccaggccg
    46321 cgctgtaggc caagaccttc atggcctcca gctccgaggc catctcggcg atgtcgtgct
    46381 gaatcgactg cacgtccgtg cccggatcgg tccgctgcat tttctcccga gcaaactgca
    46441 gggcctcgtc gaaggaggac tgcgccatgc ccagcgtcat ggaagacaga acgagccgct
    46501 cctggttcag cagcttcatt tcctgcttcc agccattgtt gagccccttt tcctccccgc
    46561 cgagaatgga ttgcgggggc acccggacgt tatcgtagac cacctcgcag gtattcgacc
    46621 cgtggaagcc gagcttcttg atcggttttg cgctgtagcc cttcagggac gtgtcgacga
    46681 tgaaggacgt gatgccgccg tatttcgatt cggcggtccg ggcgaacgtc accgtgtact
    46741 gcgtcacacc cgccccggtg atgaacatct tgccgccgtt gatcacgtaa gacccgtcct
    46801 tcggaaccgc ctccagcgcg cgcgtcttga tgctggcggc atccgagccg gcgttaggct
    46861 ccgtaatggc gaagctgaac atgatctcgc cccgggccag tttcgggaga tattcatcct
    46921 tctgggcctg attgccgttc acgccgatga tctcgttgcc gtacaggagg atgttgccga
    46981 ggttccagca caggacgggg gaccgtttgg cgatctcctc gaagacgatc atcaaatcca
    47041 cgatgtcgcc gttcaagccc ccgtaggcct cggggatatt gatcctggcc aaccccagtt
    47101 cgcagaattt tttcacgaga tcgtggggga actcgtccct ctcctcgatt tcacgaacca
    47161 cggcccggga aatgttggcc tcggtatatc cccgcacctt ctcccgcaac gcattctgct
    47221 tctcgctgaa attgaaatcc acgttccgtc tccttccaaa aacgtccggc gcctcccccg
    47281 gcagggaggg ggccgtccgc tcgggcgtca ggcgcctttc cggcgcatca gaaacttcat
    47341 gtcgaaaacc atgacggctt cgtcgcgctg gttcttcacc gtataacgcc aggtctgcaa
    47401 tccctgcgag ggcttgctcg tctccttctt ctccaccgct tcgatatgga ccgtaatcgt
    47461 gtccccgaac ttgaccgggg ccagcatgcg catcttatcc acgcccatcc aggcgatgat
    47521 cgaaccgccc tccaccttcg ccaggcccgc catgccgacc gccatcccaa agatgagagg
    47581 cccatgggcg atccgctcct tgaagaccgt ttttgcgcaa tactccgcgt ccatatggag
    47641 gggataaaaa tccatggtca accccgccca gttcaccaca tgcgtttccg tcaccgtgat
    47701 ggacggcgtc cgaaaattga aaccgatatc gaaatcttcc cagtacttct gcgacatgat
    47761 ccacctcctt gatcagttcg ccccgtccgg accgcggaat cttacgcttc caccggcttg
    47821 aacttcggca gggtgaattc ctcgttcaat ttttcgaaaa cgaccgccac cggcatttcg
    47881 caatagacgc tgtgcggatc gcagccgacg atgttgctca acatctgcac cccttcctcc
    47941 aggcggacca cggcgaccac aaagggcaga tcgggaatga aggccgaggg cggatagctc
    48001 tcgacgacgg tgtaggtata aacctttccc cggcccgacg ccggcaccca ggtcaggttg
    48061 tccgaaaagc agttcgtgca ggcgatccgc gggtagaaga tataggtgcc gcaatccttg
    48121 cattgctgga tgatcagctt gttttcccgc gccgcctccc agaaaggctt ggcccaggga
    48181 ttgacgaccg gaatcggttt gggtttaacg ggtgtatcca tgactgacct cctctcccta
    48241 cggaatttcc gttcccagga tggtgacctg ggcgtccatg tacgtccccc cgacagcggt
    48301 ctcgaccgcg aagcgcgccc cctggacctg ccgttcgccg gccttgccca tcaactggcg
    48361 cgccgtctcg accatcaggg ccacggcgcc gccggcgccc gtatgcccct gggacagcat
    48421 gccgccgttg gtcgtcgtcg gaatcttccc gccgggccag gtatcgccct tcaggaacat
    48481 ctcgccgccc tgtccccgct cggcgaaacc gagctcctcg aagccgatca gctggtagat
    48541 cggataggaa tcgtagatct ccgccacgtc gacgtccttg ggcgtgagcc cggcctcctc
    48601 gaaggccatc cgccccgcct tggcccatcc gaaccggctg acatcgggtt cctgtgaaaa
    48661 gacgaagtgg gtcacgacac ccccctcgcc caggagatac acgggacgct cgcagatggc
    48721 cctcgcccgc tcggccgagg tcacgatgaa ggccgccccg ccgtccgcca ggacgttgga
    48781 gtgcttgacc ctcagcggcg tggagagtga cttggaggcc atgacctcct cgatcgccag
    48841 atccttcttg aacatcgcgt tggggttcag ctgcgcccat ttccggttgg agacgcagac
    48901 cgccgagatg tgctcgtccg tacagcccgt ctcgtacttg tagcgcatgg tcgacatcgc
    48961 ggcgatcgcc gaatagtgct ggccgaacgg cacttcccac tccgcggaga tcccggccgt
    49021 cgagaacaga tcgatgccgc cctgcgcgga gacgcccgtc cccagacggt cggcatggac
    49081 gaacaggatc atgttcgccg cgccgctgtg gatgaggctc gcggcgattt ccagggcgca
    49141 ggtgctgctg gaaccgcccg cgaacacctg gcagttcttc tttacgttct tgagccccag
    49201 ttcctcggcc acccggcagg tcacgagttc cgtgttgaac tgggggctga acagcgccgc
    49261 cgtgggcatg acatagtcga tctcgtcctt ttggataccg gcgtccctga tggccagcct
    49321 ggccgaggca atggcgtgat agatggccgc cgtctccgga aatctccccg tggggacttc
    49381 accgatcccg atgatggcga ccttcccttt cagtttactc atggtggacc tcctcccttg
    49441 atcacgttcc gttcgacacc accgtgccct gcatgcgccg gcacgtctca ccatactcgc
    49501 gcaccatctc ggcgacgatc gcgcccgcag gcttgatgtc cctcacctgg gccacgctct
    49561 gtcccgcact ccagaaatcc agccattttt tcgaggtgag cggcggacgc tcctccgtgt
    49621 ccgcgatgct cgcggcgatc cagttcgccg gattccccgt gattcgatcc gtgcagacaa
    49681 tgccgtccgg atcggcatcg atgaccgcct gcttgtaagc cgcggaggcc cgggattcag
    49741 gggtggcaat gaaccgtgtc cccacgatca ccgcgcaggc gccgaccgcc agggccgccg
    49801 ccatctgcgc accggacgcg atgccgcccg ccgcgacaac gggcaatccg atcgtctcct
    49861 tcagatacgg aacgagaacg agcgtcgaga tccggccgcc atgaccgccg gcgccggccc
    49921 ccaccgccac cagaccgtcc acgccgccct ctctggcctt cagtccctgc gcgagcgaga
    49981 cgacgtcggc gaacacccat cccccgtgcg catgcacggc atccaggatc aggcggggat
    50041 cgccgagaga cgtaatgaag aatttcaccc catgctcgag acagagatca agctgttcct
    50101 gccagggaaa ttttccggag aggtggatgt tgacgcccac cggcgcctcc gtccgggagc
    50161 ggatgatgtc cagagcccgc cgcagttgat ccgtcgtccg gtaattcggc aggggaagca
    50221 tgccgagccc cccggcctcg cagacggccg cgacgagttc ctcgtaggaa accagaaaca
    50281 tgggcgcccc gatgatggga taacggatac ccatcacggc ggtgaattct gtttgaatca
    50341 agggcggcat tcgggttctt gcgctcctct cacccttgca gaatcatcct gtatcgccgg
    50401 ggggcctcgc cgtccgtccc ggcacgcccc ccggtccaac ggggggcctc aggcttcgtt
    50461 cctgccgacg atggcaatgc tgcagacgtt catgaacggg aatccgccca ggttgtgggt
    50521 cagaccgtat cgggggttct cgagctgccg ctggccggca cgtccgtgaa actggaggta
    50581 catctcgtaa atcatcctca gccccgaggc gccgatcgga tgtccgaagc acttgagccc
    50641 cccgtccacc tgacagggca ctttgccgtt cagatcgtag aagccgtcca tgatgtcctt
    50701 cgtcgcttct ccccgcgcgg agatgtgcag gtcctccatg gtcacgagtt ccgtaatgga
    50761 gaagcagtca tggacctcca tcatgctgat ctcctcccgg gggttcacga tcccggcctc
    50821 ccggtaggcc gcggcgcttg ccttggccgt cgtgacaaag ccggagccgt cccagttggt
    50881 gaaggacatc tcctcgccgt tgctcagcga catctgcagg gccttgaccg tcacgaaatc
    50941 cttcttcccg aggcgccggg ccacctccgg cgttgcgacg atggcgcagg ccgaaccgtc
    51001 actgacgccg cagcagtcga agagtcccaa gggataggcg atcatggggg ccttcaggat
    51061 gacctcctcc gtcaccggcg cccgcaggtg cgccttggga ttgagggcgc cgttggcgtg
    51121 gcttttcgca gagacgtggg ccatcgcgcg cttcagttcc ttcatgtcca ccccgtattt
    51181 ggcgctgtaa ccgctggcca gcatggcaaa gcaccccggc gcggtcatgt tggggaacca
    51241 ctgctggttg gtcaggccca tcccggccga aaattccggc agccccgcat acccgatatc
    51301 cttgagcttc tcggcgccga tggccaggca gatgtcatag gcccccgaag cgacggcgta
    51361 gacggcgccc cggaacgcct ccgtgccgct cgcgcagtaa ttttccaccc tcgtcacgcc
    51421 gatgttgggt agacgcagag ccagggaggg cggcagcgcg ctcttcccga cgttcacctc
    51481 atccaggcag gagccgaacc aggccgcctg gaggtcgttc ttctcgatcc cggcgtcgct
    51541 caagcactcc tgaaacgcct ccaccatcag gtcttccgga ccgcaatccc agcgttcgcc
    51601 gaatttggtg catcccatgc caataatggc aaccttgtcc ctgattcccg tagccatcgt
    51661 cacaccccct tctcggtcgc ctgcgggacg actttccaga aatagccgct gaacccgcgc
    51721 accgggtctt tcgacctccg gcggaacgac atccggacgg gaagccccac cttgatctcg
    51781 tccagcgtac agtccgtgaa atcggccagc agccgacccc cgccctcaat gtttacgatc
    51841 ccgtagatcg caggcggatc gaccgatgcg gccagcatat cgcccgtata catgaagacc
    51901 gttcccttct gaccggagaa cgcgtagtct tccatatcgc cgacggcgtt gcagtccgga
    51961 ttcacgcaga ccggtgtaaa ggtgtattga ggcgttccgc attttttgca gcggctgccg
    52021 accaagccga tgagtttctt gcggttccgc cacaggatgt tgagcgacga attcggattg
    52081 gcctcgccgc ggatgcccag atccgcggtg acgatatccc ggaacttcat gaacttcgta
    52141 taactcgtca attccgcccg cttcgccagc gagcccttga cgccctgacc gccgcgataa
    52201 cccgccatcg cgtccgtgac ctggaaatac aggacgtcgc agccctgacc gaaccccacg
    52261 aggaggatct tgtctcccgg cttggcgtct tccagggccg ccacgaacat caccagcggg
    52321 tgcgccgcgc ccgtatctcc gcagacctcg tgaaggttgc tctgcacctt cgcaggatcg
    52381 agcccgagct tcttggcgat ccccctgtgg gtggcgctga agtagcaggg atagacgacc
    52441 ttgtcgaagt cgccgagggt cagacccgtt ttcttcagaa agccgaaaat ggcccggggg
    52501 atgatcttgc cgtatccctc atccctgacc catcgttcct cccacgcata gtcgaactcc
    52561 ttgccggcgt cgcgataatg atcgatgaaa tcgcagctga gggaatagcc gcccttgaat
    52621 tcggcaatca cctcctgact gcccagcagc agggcggccg ccccatcccc cagaaacatt
    52681 tcgaaattgg tcgccatctt cgttcgccgc tggtcgcccg ccgccacgag gacgcgcttc
    52741 tttccgccgg cctgaatcgc ctccagggcc gagatgacgg ccgtcgttcc ggccttcagg
    52801 cagcccgtga aatccgcaca ggccgtcccg tcctcgctca ggttgagcgc cgtcgccagg
    52861 atgtcggcgt tgagacggtc cgcaaacggg aaggtcgtcg aggcggcata gacgccgtca
    52921 acctgcgttc tgtcccgccc gacgagacag tcctgcgcgg ccgccacggc catgctcagg
    52981 gcgtcttcat cccagttcgc cacggccttt tctccctgag cggcgcccca gatgaccggc
    53041 gagtaccacg ccacgttctg ggcaatcagc ttccggctca atcgcaagcg gggaatatac
    53101 cccccaaaag atgtgatacc gatcatgatt ctcctcctca ccaacgaacg cttcgcgcct
    53161 tccccgcatc tccggcctgc cgccgggctt cagcggcgcg acacgaaact ccccgaaaag
    53221 gcaccccgcc cggacgcacc cccgcccgcg ggcggaaagc gcatccggga catccccatc
    53281 gagaggccct cactttttga tcgcggccag ctcctcgaca atctgcttgc atttcttgat
    53341 gagcccgcgg gcctccatca ggatcgtcat ccgctcggcg tagggcgacc cctccccgtg
    53401 gagacagatc gtctccaggt ccgccgaggt catccggcgg atgaggtcga acatgcgcag
    53461 acgcgcttcc gtcggcaggc cggccttccc acccagatac ttgtcgatgt cgtcgtgcgt
    53521 ctcgggattg caccaatcct gatacgtcgg ggcggtgacg agcagtccgc ccgccaggtc
    53581 ctgaacgatc tcgacgatct tgtgataatt gtgggcaaag tggtactttg cgatgttcgt
    53641 ggtcatggga ttgggcaccg gaatgcccgc atggtccacg aagtcgagac aggcggaccg
    53701 ggacagggcg gaaagggttt ccagataaat gacgcactcc gtgatctttt ccctgacatg
    53761 cggggcgtcg gccacaccgt tgtaatcggc gatcgccacg gcggccccca ggagctgctc
    53821 gctcagggga atccggtagg cgcagcccgt gcggcggtgc atcagggcga agttgtagac
    53881 gatggaggcg gcgaacttca cctcgccgca caggaagacc cgctcccagg ggacaaagac
    53941 atcgtcgaag atgatgaggg agtcggtgtg catccggcgg agctcccgct tgaccggaaa
    54001 ctccatgtcg ctgatccgtc cgtggaaggg acgaacgatc tgccggatcc ccttggcatt
    54061 cgcctggatg gcgaaggcca cggcgtaatc cgcatccgcg tccgtcatgg cccggcaggg
    54121 gatgacgaaa aattcgtggg cataggccgc cccggtgata tggaccttgg cgccgcgcac
    54181 gatgatgccc tggtcgttcc gctccacgat ccggacgttg aaatcggggt gattctgctt
    54241 cggatcggag ggccgcaggc ttcgatcgcc cttgacgtcc gtcacgcccg cgacgatgca
    54301 cttgtccgtc tttttcaggt acgtgcggaa gttctcgatg cgctccaggt acaccttgcc
    54361 ggcatccttc atcgcccgcg ccgtgatggc gatggcattc agggcgtccg caccgatgtc
    54421 gtgggccagg ggaatatagc cgtcaccgag ttccgtggcc ttgatgatga gttcgagctg
    54481 tttgaggagg tcctgcccgt tctgcggcgt gtggtaatag cggctgatat tctcgcccaa
    54541 ctcctcatcg tacaccaggg caagctcctt gtaccgggga tcgtgggcca tttcgtaatc
    54601 gacggcgagg gtctcgacgc aggccttcaa atccggatcc gtggcgacat tgtcgatctt
    54661 tcgtcccttg tagtagacga tgcgcccgtc attcaaactg tccttgtact cctgtgcggt
    54721 cattaaaccc atggtaccca tgcctcctta tgcattctct ccggctgagg gatgaagtga
    54781 agatccatac tcagatcgat ccgccgccgt cgacgatcag gatctgcccc gtcatatagg
    54841 ccgaggcatc cgacgccagg aacagggcga tccgcccgat ctcttccggc tcggcgatgc
    54901 gcttgatcgg cgtgtgggcg agttcctgct tcatcatcgg ctcgttgctc cagagggcct
    54961 ggctgaactt cgtccggacc acaccgggag caatgcagtt gacgcggatg ccgaaatcgc
    55021 ccagctcctt ggccatcgcc cgggtcagca tgccgatccc ggccttgctg atacagtaca
    55081 accccagtcc gacatcgggg ctcacgccgc cgacggagga gatgttgacg atgttgccgc
    55141 ccttctgctg ctgcatgacc ttgcccacga gctgactcag gatggtatag cccttgaggt
    55201 tcgtgatcat gatctggtcg tacatcttct cgtccatgtc gacgagcagg cccatgccgg
    55261 gattggtggc cgcattgttc acgagaatgt cgatgcgacc gaattccttc acgacggtat
    55321 cgacgaaatt tttgatgctt tcaacttccc ggttgtgcat cgccaccgcc agggcctttc
    55381 gccccatggc cctgatctcc tcggcgacct tttcaagatc ggccagcttg cggctgccga
    55441 cgacgacatc ggccccggca tcggcgagcg ccaacgcaac ggccctgccg atgccccggc
    55501 ttcctccggt gaccacggcg acctttccct gcaacgacag ataggatgta ttcacacttt
    55561 ttctcctttc cgaatgattg atggcctcct ggaacgcgta tcccgtctcg ttagcaaaat
    55621 ggatgccacc tctcatttat aaatgaatct ccgtgatttc aaatgcttgg catttaagcc
    55681 gccccaaaaa acgctcgaga ggcacaacat acggtttctt gaccctccac atgttgcgcg
    55741 ggtgcgcgtc gggggttgtc cccccccccc caagggtcag ggtcccggtg gcgggcacat
    55801 ccgcctccgg gaccctgacg ggcggcatgt ccgaccgccg ggtcgagcga tttacccggt
    55861 cacttctggg gcgtccgcgg cgccttgatc gaatcgatga tgacgaaccg tttggccttc
    55921 ccgtccgcct tgaggacaag gctggaaaag ctgctcacgt gctgcttcgg accgaaggtg
    55981 atgttcgggg aaagtccgcc catgtcgaag ttcttgacgc tttcgagccc cttgacgagg
    56041 gtttcggggt tcagattccg cccggccttg cggagcccct ggcccatggc ttcgatgtac
    56101 ccccaacccg ccagatacag ataatccgga tacatggggt tgcccagagt cttggccgga
    56161 ttgccgtatt tcgcgttgat ctccttaatg cgcttgatcc ccggaaggct catgtgtccc
    56221 ggggcgtagg gcgtgacgag catatagccg tccgccgcct ctcccgtgag ggcaaacagc
    56281 ggcggaacac ccgtcgtgtt gttggagatc agctggacat cccagcccat cttgaccttc
    56341 tgcttgatga tggcggccga ctggggcagg tacgtccaga ggacgatggt gtccacgttg
    56401 gcctccttca tggcgttcat gtgcggggtg aagtcgatgg ttccggtctt gtacggggcc
    56461 tcagccacca gcttggtctt catgaacttg gccgccgcgc gggccccttc cagaccttcc
    56521 ttcccgaaat cgtcgtcctg atagatgatc ccgatcctgg ctttcctgtt tttgccgagg
    56581 atgtattcga ccgcacggga tgccatgctg aactgaaccg tatgggggac gaagacccat
    56641 ttgtttcccg gggagtagaa ttccttgttg gccccatggg gcatgaggac gacctggttc
    56701 tcctcgcaca ggggcaagat ggctctcgcc ccggcgctac ccaggttcat ggcgatggcc
    56761 agcacctcgt ccttgaggat gaccttcttg gcggcgttca tggtcgtggt gggcaggatg
    56821 ccgttgtcct cgacgatcag gttgatcttc cgcccgttaa tgccgccggt atcgttcacc
    56881 caggccacat aatcccggaa ggcgtccgca atgggcggtc cgccatactt ccccgggccc
    56941 gagaggtcga cgagacaggc gatcttgact tccttgtccg tcacgccgcg cacgtccgcc
    57001 gcccaggcgc tgcccagcga caggcacagg gccaccgcca acacaacaca ccaccattcc
    57061 attctcgctt tcatttttcc cctccctttc gtgattggat cattcgattt cgccatcgtt
    57121 ccttccggtg accgatggcc catgctggct tccgatgtcc gaagaattcc ctcttccccc
    57181 ggcggaaccg ctggtgacgg accgaccggg acccccgccc gtcaatactt gaagggccag
    57241 gtcttccagt agaccttgat ccgacgatag atcccgaaga ggccctgagg ttcgaacagc
    57301 agcgtgagaa cgatgatgat accgaagatg cccagtttga tatccacgat cagcccgctg
    57361 aggctgggat acgtttcgcg aaaggcgtcg gtcagaaaaa cgatgccgtg gggaatgccg
    57421 gtaatcagga tggccccgag gatagacccc aggatggttc ccatcccgcc gagaacgatc
    57481 atggcaatat acgcgatcga cagggagatg tcgaagtttc cgggaacgat ccacttctgg
    57541 taatgcgcca tcaggcatcc cgcgatgccg gcgtagaagg cgctgacggc aaaggccatg
    57601 atcttgtact tcgcgagcga gatccccatg gcttccgcgg cgatatcccg atcccggatg
    57661 gccacgaagc accgtccgat cttggtacgg gcgagattct tcgctacaag cgtggccagg
    57721 accgcgaacg acatgatcag aaagaaatac tgtcgctccg tttcaatcac aaagcccccc
    57781 gcgccgatct tgggaaccgc cattcccata tcgccctttg tgaggtccca gtggaagagg
    57841 acgtattcca cgatgaagcc gaaggccagc gtccccaggg ccagatagag gcccttgagc
    57901 cggagggagg ggatccccac gatcatcccc agcagggcgg ccatcagacc ggaaaggggc
    57961 agggcgatca caaagggaag tccgagcgcc cccgtcagga ggccggtggt gtaggcgccg
    58021 atggccacga aggccccgtg tcccagcgaa atctgccccg tgtagcccgt caacagattg
    58081 aggcccaggg cgccgataac ggcaatgccc atctcgttca ggatgctgag ctggtagcgc
    58141 gacatcagaa agggaagcgc gatcatggcc gccacgaaga tcgcgagcca ggatttgacc
    58201 cagagattct ggaacagcgc cgtatcttcc tgataactga ccttgaagtt cgccatgacg
    58261 gtctctctac accctctcca ccttttcccg gccgaagatc ccgtgcggcc ggaccatgag
    58321 cacgaggatc atgatcgcga aggcggccac atccttgatg tccccgaaat ggaagttccc
    58381 gagttccatg ccgctcagat agctcgcggc gaggccttcg accaggccga tgaggatccc
    58441 gcccagaatg gcgccgctga tgctgtccat ccccccgaga atcgccgccg ccatggcctt
    58501 gatccccgta tggctcatgg aaaggcggac gaaactctgc tccgcgagga acatgcccgc
    58561 cactgccgcg accagcgccc ccagcgccca ggccgccatg tggatccgct tcacgttgat
    58621 gcccatgagt cctgccgtat cgggatcctc ggccgtcccc cgcatggaaa tccccaggag
    58681 cgaacggttg aagaacagga caaacagggc gaaaaggatg acggatacga taatggtaaa
    58741 aaacgaaccc tgggcgaaga taatcccccc gagattgaag atcttgtccg tgaaggggga
    58801 gcggagcgag tagacgtcgt gcccccagat gagaccggcc acacaggcaa ggatgctcgc
    58861 gagcccgaca gtcaccatga tcgtcgagaa caggggctcg cccaccatcg tccggaagaa
    58921 gacctgatag acgattgcgc ccagaagcag gccgccgatc agggcgatga agaaggcata
    58981 aacataagac acgcccatga gcgtcgcgaa gctgtagcag atgaaggccc ccaccatggc
    59041 cagttctccc tgggcaaaat tgatgacgcc cgtcgatttg aagatgagga cgaaccccag
    59101 ggccagcagg gcgtaaatgg cccccatgga caggccgttc aacaggatct ccagaatttt
    59161 ctccatgcat ccacccgcct tcctgatccg gacgtcacga ccgcggtctc agcgtcccgc
    59221 gtacatcgcc tcgatttcct tttggaaccg ctcttcgatg attttgcgtt tcaccttctg
    59281 cgtcgccgtc agctcctcgt cgtcctggtc cagctccttc tccaggatct tgaatttctt
    59341 gacggtctcc acctgggcga gaccttcgtt ggcggcatgg acctcttcct tgatcaggtc
    59401 gtatacggcc ggattctccg ccaggctccg ataggtcgta aaggggatct tgttcttctg
    59461 cgcccagttc cccacgttgt cgtaatcgat ctggatgagg gcggtgataa aattgcggcg
    59521 atccccgatg acaatggcct ccttgacata ggtgctgcac ttgagcttgt tttcgatctc
    59581 cgagggagag atgtttttcc ccccggaggt gatgatgatg tccttcttgc ggtcgacgat
    59641 gtaaagatgg ccgtccgcgt cgagttggcc gatgtcaccc gtatggaacc agccgtcggg
    59701 aaggacatcg tgggtcgccg ctggatcgcg gtagtatccg acaaagatcg aatccccgcg
    59761 aagcagaatc tctccgtcct cggccagctt gatctccagc gcacggcagg gctcgcccac
    59821 ggagccgtgc ttgatttggc cccggggacc ggaaaccgtc cccgccgttt ccgtaatccc
    59881 gtacatttcg atcgtccgta cgcccaatat tcggaagaaa ctgaggattt ctggtgcaat
    59941 gggggcgccg ccgctcatga ggcaacggca gttgagcatt ccgatcttgt cccggatggc
    60001 acggaagatg aagagatagc ccagggcata caggagccgc cagccccaag gaaccggcgc
    60061 gccgctcatt tcaaactgcg agacgcgacg cccgaccggt tgccaggccc gatacatcca
    60121 atgcttgaac cacgttgaat cccccatccg gatttggacg ctcgagtgga gcttttcgag
    60181 gatgcggggg acggcggcaa aaaatgtcgg gccgatttcc tgaatgttct gctggaccgt
    60241 atcgatcgac tcggcaaagt tgaccgtgta gccgaacacc aggggcatcg ccatggacag
    60301 gtttcgctcc aggatatggc acagcggcag ataggaaagc agggtatccc gatccgtaaa
    60361 gcgcaggatc ggctgcaggc cgttgacgat ctggtggatg atgttccggt gcgaaatcat
    60421 ggcgcccttg ggatgccccg tggtacccga ggtatagacg ataaaggcca cgtcctcggg
    60481 tcgggtcgct gccacgagcc gggaaaagcg ctccgggtcc tgacgggccg cctcgcggcc
    60541 ggccgcttcc acctcccgat agctgacgat gaggggctcc tcgtaatggc gcagcccctt
    60601 catgtcgatc acgatgatct tcgcaagccg ggacagcgac tccttggccg cgagcacctt
    60661 atccgcctgc tcctggtcct tgacgaccac gaaccgggat tcggagtgat cgaggatata
    60721 ccggacctgg tcggccgaat tcgtcggata gatgccgacc gccacgccgc ccacgccctg
    60781 gatcgcgagg tcggtaaaga tccactccgg gcagttctct ccgaggatcg agacctggtc
    60841 cccctttgtc atgcccagcg ctacaaggcc caaggagaag gcctcgacct tgtcccggta
    60901 ggtgttccat gagatttcct gccagatgcc cttgtatttc tcccgcatgg cgacccgccc
    60961 cccatagagc cgggccttct ccaagaggag tttcggcagc gtggtctcta ccctctccat
    61021 gaatcccttt cccccaaccg gtcgggacaa ggcaacggtc cgcttgacgt catcccgcct
    61081 ccccgaggta cgcctggacc accccgggat ttctgctgac ttcccccgga acaccctcgg
    61141 cgattttggt gccgaaattc aggaccgtca cgcgatcgga caggtccatc acgaggccca
    61201 tgtcatgttc gatgaggatc accgtgatgt ccctctcctc gttgatgtcg atgatgtacc
    61261 gcgccatgtc ctcgcgctct tcgagattca tgccgctcac gggctcgtcg accagcatga
    61321 gcttcggctt catggccagg gcccgggcca gttccactcg cttctgcagg ccatacgaca
    61381 gactcccgac cggcgatttg cgcaccggct gcagatcgag aaactcgatc acgtcttcga
    61441 cttcgtcgcg attctgaacc tcttcccgaa gacagcggcc gaggtaaacg gcggccccga
    61501 tcaacccgta cttcatgagg tggacgcgcg ccgacatcag attgtcgagg acggtcatcc
    61561 ccttgaacag ggcgatgttc tgaaaagccc tggaaattcc cagttgcgcg atccggtcgg
    61621 gcttgaggcc gatcagttcg cggctctcaa agcggatgct gcccgttttg gcccggtaaa
    61681 tcccgctgat gcagttgaag atggtggtct tcccggcccc gttcggtccg atgatggaat
    61741 agatctcgcc cttcttgacc ccgaaggaaa caccacgaat ggcctgaatc ccgccaaaac
    61801 tcatggacag atccgcgatc tcaagctgaa gggaggcctc ttgattcatg acagccatcg
    61861 tttccgtctc ttgtaatgtt tgacgtcccg gaagctcttt ttggtgccca catccgtcag
    61921 ccccaggtaa aattccttga tgtcccgatt ttcgcgaagc acgtcgctgg gaccttccat
    61981 cacgatccga ccggtctcca taatatatcc ataatgggcg atcgacaggg ccacgttggc
    62041 gttctgctcc accagcagga tggccacccc ccgctcccgg ttgatatggc cgatgatgtc
    62101 aaatatttct ctcacgacca ggggcgccag tccgagggac ggttcgtcca gcatgatcag
    62161 ccggggctcg gcgatgaggg cccgggcgat ggccagcatc tgctgctccc cgccgctgag
    62221 atagccggcc tgctgccgac gtcgttcctt gagaatcggg aaatagttga agaccatctc
    62281 ctcgttgcga atgaagtttt ttctctcctt cagggcatag gaaccgatga ggatgttttc
    62341 ttccaccgtc agttcggcaa agatgcggcg tccttccggg acctgtgaga tgcccaggga
    62401 gaccacctcg tgtggctcga gggcgccgat gtcccggccg tcgaactcca cctttccgtc
    62461 ctcgatctta aggtcgatgg cttttcggat tccggagacg gtgttgatcg tcgtggtctt
    62521 gccggcgccg ttggcgccga ggagggcgac gatgcgtccc gccggaacct ggagggaaat
    62581 gcccttcaag gccagaatga tgtcgttata ggtggtttcg agattggtga ccctcagcac
    62641 cagacccccc tggttcccat cggaaaatgt caacgcctga cgcacaatcc gtccgatgat
    62701 cctgtccccc gttggatgaa ggccgatggc ccaaggcacc cgccgacaac cccgaaagac
    62761 cggggcgtct cccggaccac ctgtttctgg aaggtgggat cggcctgtct tctggatcta
    62821 tcattccacg caatattgaa gccaaatatg tcaaccactt gttctcatta attaaaattg
    62881 aactttgtcc ctcacggaca cccgggggac caggtcaatt gcgcaacata ttgtttttgg
    62941 ccatcgggat gttgtgcact gatccttccc ccgcctggcc gagggtgtcg ttccagcggc
    63001 accccatggg aaaaaccgac ggcccggaga ggtcgaacca ccatggaacc cggggtcatt
    63061 cctcctgcgg gtttcattcg gcaaccggcg ttgccggagg cacaggcatg taaattgcga
    63121 gttatccaac aggcaaattc agggccgacg gtgtcgaagc atgatctaca tcaaggaaaa
    63181 gtttgtcgat gaccgcacaa tcgtcatgaa ggtggacggg gtactggacc aggacgcctc
    63241 cgccgttctc aaccatacat gccagaaccg ccttcagaca aaatacagca tcatcctcaa
    63301 tctggagggt ctcatccaca ttacccgcga ggggagaacc tttctccagc aggttcagga
    63361 cgggatccgt ttggagaata ttccggattt cgtaaagctc gaacactgaa gccctcccct
    63421 cccccgcccc aatccgccgc aatcccatca cggccggata acgccgtccg gggcagggtc
    63481 cttgattgag ttcacgtctt gtgatagcgt tcggatcagg ttcaaatcgc tgccgtccgt
    63541 ttcggccggc aaccgttgcc cgcgggtttt tcaggggtaa aggagaccgg aaacgtggaa
    63601 acccatcccg tgcagaacga ctaccagcgc ttgctggcca ccgcggcgct ttttcaggag
    63661 accttttcga tcgactggct gatggatctc tttgcggaca ggaaaccttc cgaaatcctt
    63721 tccctgctcg ccaacggcgt cggtgagggt gtgctgtgcc aggagggtcc gggtcagtac
    63781 cgcttttcgc atcccaagaa gaaggacggc tggaagcagc gcctttccga acaggaagag
    63841 acgattgcgc acggtttcat cgcggacatc ctgatgcgcc agttgccgga cgacgatcag
    63901 aaggcccacg ccttggccca tcacctcatc catatcgtca acgacacgga acggtgccaa
    63961 tacctgaccg ccgccggcga tcttcacctg aacgcctttc gggccgaaga cgcccttcag
    64021 tgctacacca aggttctcga cgacctctcg acccggcacg gcgaggatga ggaccgcctg
    64081 tttgcccgaa ccgccattca gtactccaag gtttcgaccg cccgccatga caccgctcag
    64141 gtcctcgcca tcctgcaggc ggccctggaa agggcggaaa agcgcaacct gcagagcacc
    64201 caggcgattc tcaagatgca catggccaag aacgagtggc tccgttcccg ctatgacagc
    64261 gccatgcatt acttcgatca gggctgggcc ctggccaagg aactgaacga accgagattg
    64321 atgcgctcgg cgatgaattt ctccaccttt ttcctgtact ggcaaggacg cttcaaggat
    64381 gccatccaaa tctacgaaca gtccgtttcc gacatcgaaa actatccccg ggaaagtttc
    64441 aatctgttgg ccaatatcac catcggctac tgctacgccc agatcggcca ggtgacgcag
    64501 gggctgggga tgctggacgc catccggaac cactgcctgg agcgggggga tctctacctg
    64561 gcgtgccata ccgcgggaaa catcggcaac atcctgctcg acatcggcag gcgggaagac
    64621 gccctgtcct ttctggaaac ctcggggcgg gaagcgaaga aatcccataa cgactgggtc
    64681 gggatgtccg tcaacatcaa tctgtcctat ctttactacc ttcggggcga cacgaaaccg
    64741 gccgtccggc acctcaagga ttttctgcag caaagcaaga aagtccaggc caccgtatac
    64801 ctgtacccgt acctgctgga actttgtctc gccatgaaga cgggcaagct gccccagatc
    64861 gacggtctct ccctcgagaa ggaaatcaac gtcctgatca aggggaagaa tatctttctc
    64921 aagggggtgg cctatcggtt cctcgccatt gtccggaaga tggaaggcgc ccccccggag
    64981 gagatcatcc ccattctgaa caacgccgtc cggtggctgg acgaggcggg gcatcagatc
    65041 gagctgtcca agacacgcct ggaactggcc cgccaatacc tggccagcgg ggaggaggac
    65101 aaggcgcagg aactgatgca gatcacgagc aagatcctcg ccaccttcaa cgaagagatg
    65161 gttccggacg acctgcgggc cctgatcaag aaccggccgg tgggggaaag cctcctgaag
    65221 gagattatca agctgggtca tgaagtggtc accatccgcg acaacaagga tctgatccag
    65281 cacatcatca ccaccctgaa ccggatcacc ggcgccgagc ggggggccat cttccttttc
    65341 gaggatgggc gttcgccccg gaagctcctc ctccgggcct ccaagaattt gacccaggag
    65401 cagatcagcc atgagaactt cgcctcctcc atgaagatga tcgacgatgt cgccgcgacc
    65461 ggccagggaa gaatcttcga ggcggacctg gaaaaccagg ccctgtcggc cggcgaaatc
    65521 atccgctccc gcatctgcgt ccccatgatc ctgagggata agaccgtggg ggtgctctac
    65581 cacgacaacc ggcttctgag cagcgccttc aaggaatccg acctcgatct catctcctac
    65641 tttgcggcgt gggcggcctt cgccctggac aacgtcagcg cctacgaaga aataaagcgc
    65701 ctgaaccgaa agctctgcga ggagaacctg tactacaagg aagagcacct gaagaacctc
    65761 cacttcgagg acatcgttgg cgagagttca ggcatcaaac atgtcctgag ccaggtggag
    65821 caagtcgccc agacggacgc caccgtcatg atcgtcggag aaaccggcgt gggtaaggaa
    65881 ctggttgccc gggccatcca ccgccacagc gcccggcggg acaagccctt catccgggtc
    65941 cattgcagcg cgttgcccga gagcctgatt cccagcgagc tgttcggcca tgaaaagggg
    66001 tccttcacgg gggccattcg gcggcgtctg ggcaggttcg agctggccga cggcgggacc
    66061 ctgttcctgg acgagatcgg cgacatcagc ccggacatcc aggtccgcct gctgagggtc
    66121 ctgcagacca aggagttcga gcgggtcggt ggatgcgaaa ccttgcgttc cgacttccgc
    66181 ctcatcgtcg ccacgaaccg caacctggaa gggctggtca agaatcagac cttccgctcc
    66241 gatttgtact accgactggc cgtgttcccc gtctatgtgc cccccctgag ggagcggaaa
    66301 gaagacatcc cccggctggc gtattacttc ctgaggattt actcccagaa gcgggggaag
    66361 gaattttcca ggatcaccga ggcggagatg gagaagctca tccagtacga ctggccggga
    66421 aacgtccgcg aactggaaaa catcatcgag cgcggaacga ttctctcgtc cggtccgatc
    66481 ttcaatgttc cggaactggg tgccgtgcag cacccggaaa ccggaccccg caaggacgtt
    66541 atcaccctgc gcgacaacga acggcgccat atcctctggg cgctggagaa aacggcttgg
    66601 aaagtccgcg ggaagggtgg tgcggcggag cttctggaca ttccgccctc cacgctggcc
    66661 ttccggatga acaaactggg catcaaaaga accccggccg ccccggttca gccctaggat
    66721 cttgattttt tgcttgacat tcacccgcga tctgaatatg agttatcgaa tatccatcat
    66781 ttgattgaat cgtgaaatgg aatagagcgg tggagtgcgc cgcctcgatc gctgaaaatt
    66841 actgtaggga gggaaacgta tgtccagcca gttgaggtac gccgaggttt acgaacaatc
    66901 cattcaccag ccggagcttt tctggggtca ggcagcggaa gagatccagt ggacgaaacg
    66961 gtgggaccgg gtgctcgacg acagcaacaa gccgttttat cgctggtttg ctggcgggga
    67021 actgaacacc tgcttcaatg ccttggatta tcaggtggcg tcgggtcgcg gtgagcaggc
    67081 cgccatcatc tacgacagcc ccgtcaccgg aaccgtccgg aagatcagct atcgggagat
    67141 gctggatcag gttgcgaaat tcgccggcgt cctgaccgct cagggcgtac gcaagggcga
    67201 tacggtcatc atctacatgc ccatgatccc ggaggccgtc atggcgatgc tggcctgcgc
    67261 ccgcatcggc gccgttcatt ccgtcgtctt cggcggcttc gcccccaacg aactggccat
    67321 ccgcatcaac gacgccaagc cgaggctgat cgtgaccgcc tcctgcggca tcgaggggaa
    67381 aaagaccatc ccctacaaac ccctcctgga cgaggccgtc aaactcgccg atcataaacc
    67441 ggagaagtgc atcatctatc agcgccccca ggtaacggcg gagctcacgc ccggccgcga
    67501 tctggcttgg gacgccgtga tggccgacgc caaagcggtt ccctgcgtcc ccgtcgccgc
    67561 cacggacccc ctgtacatcc tttacacctc cgggaccacg ggccgcccca aaggggtcat
    67621 gcgggacaac ggcggtcatg ccgtggccct caagtggtcg atgaagcaca tctacgacat
    67681 caagccgggt gaggtgtact gggcggcctc cgacgtgggc tgggtggtcg gccattccta
    67741 tatcgtctat gccccgcttt tgaccggctg caccacggtg ctctatgaag ggaagcccgt
    67801 cggcaccccc gatcccggcg ccttctggcg ggtcatctcc gagcacggcg tctccgtcct
    67861 cttcaccgcc ccgacggcct tccgcgccat caagaaagaa gatccgcagg ggacctatct
    67921 gaaaaagtac gatatccgtt ccctccgcta cctcttcctg gccggggagc gcctcgatcc
    67981 ggacacctac cactgggcga gcgacatgct caagatcccc gtggtggatc actggtggca
    68041 gacggaaacg ggctgggcta ttgcggccaa ctgcatgggc gtcgagccct tccccatcaa
    68101 ggcgggctcc cccaccaagc cggcgcccgg gtacaacgtg aagatcacgg atttccgcgg
    68161 cgaggagctt cccaacggac aggagggcta tgtcgtcatc cgcctgcccc tgccccccgg
    68221 ctgcatgccc accttgtgga aggacgacga acgtttcctg gaatacgtca acaagatccc
    68281 cgactgctac gtcacgggag acggcggtta ttacgacgag gacggctata tcttcatcat
    68341 ggggcggatc gacgacgtca tcaacgtggc gggacaccgc ctatcgaccg gcgccatgga
    68401 agagatcgtc tccaagcata aggacgtggc ggagtgcgcc gtcctgggcg ttcaggatac
    68461 gttgaagggc gaagtgccgc tggggttcgt cgtactcaag gcaggcagcg accgagatcc
    68521 ggcggagatc gagcaggaac tcgtccggat ggtccgggac gagatcggcg ccgtggcctg
    68581 tttcaagcag tgcgtcgtcg tcaaggccct cccgaaaacc cggtccggca agatcctccg
    68641 gagcaccatg cgcaagatcg ccaacggcga ggacttcgat gtcccgtcca ccattgagga
    68701 cgcctccgtc ctcgggagca tcaccgacgc cgcgcagaag atcggctacg ggaagaaatg
    68761 acgggacagc ggggaacccc gatccagacg ggcgccccgg tccttttcca acccgacctc
    68821 cccgcttcgc ctggggtggg gcgggaaccc ccgaacatcc gcgggacacg cttccgggat
    68881 ccgtctcccg aagccgccga aggatagaag gcttaatccc tgactggaaa cacccccgcc
    68941 tcacgaacga ggcgggggtg tttttgactt catttttcgg gccgtgatta cgcggcggtg
    69001 atcttcccct ccgccttcag cgcggacttc agctccttct tgaggatctt gcccaggatg
    69061 ttcttgggca gcgccgtccg gatatccacg tatttcggaa ctttatatga ggccatgttg
    69121 gcccggcacc agtctttgaa ggcctcttcc gtcagcgcct gaccgggatg gagcacgagg
    69181 caggccatca cctcctcgcc gtagatcggg tcgggcgacc cgacgaccgc aacctccatg
    69241 acctggggat ggcggtagat gatgccttcc agttccttgg gatagatgtt ctcaccgccc
    69301 cggatgatca tctccttctt ccggtccacg atgaataggt acccctcctc gtccttgtag
    69361 gccatgtccc cggtgtgaag ccatccgccc cgcagggtct gggccgtctc ctccggcttg
    69421 ttgaagtagc ccttcatgac gtgatcgccc ttgatgcaga tttcgccgac ctccccctgg
    69481 gacacttccc ggtcctgctc gtcaaacagc cggatctcgc tttccgcaaa ggggatcccg
    69541 atggaaccga tcttccggac gccgtcgatg ggattggccg tcgccccggc ggtgccttca
    69601 gtcagcccgt agccctcgat gatgatcatg ttgaaggtct cctcgaactt ccggatggcc
    69661 tccacgggca tgggggccgc cccgcagacg ccgtagcgta gcgaggacac gtccaccccc
    69721 tcgaaccccg gcgtgttaag gagaatctga tacaccgtcg gcacgccgct gaagatcgag
    69781 acgcggtatt tcgcgacgca ctcccagaac tccgaggccg agaagttccg acgcaggatg
    69841 atctgggacc ccttcgacag cggagacgtc ccggtgatga cgaggccgtt gacgtggaac
    69901 agggggagga agcacatgat gacgtcgttc tcgtcgatat tggcgagcag gctcgcctgc
    69961 caggagttcg tcaggatgtt cctgtgggtg agcagcaccc ccttcgggtt gcccgtcgtc
    70021 cccgaagtat agacgatcgt cgccacgtcg tcgaggtcga tcccgacggg ggtgagatcg
    70081 gccgccaaag cccgcaggtc ttcgaacttc aggaaccggt cgtcggtcgc ctcccgttcg
    70141 atgacgtgct tcagggccgg cgtcccgtcg aggatcttgt cgacgtgccc ccggtagaca
    70201 tggtcgcaaa acagggccac cgccccggaa tcgttgagca ggtactggat ctccccggtc
    70261 tgccaccagc agttgaccgg accggcgatc gccccgatct tgatgatgcc gaaataggcc
    70321 tcatagaact ccgggctgtt gtccatcaga atgcagaccc gttcgccctt ccggaccccc
    70381 agtttgagaa ggccgttggc gacccgattg ctcccctcgt cgagctcccg ataggtgatg
    70441 tcccgttcgt aatagcggat ggcgatccgg tccccgtagc ggtgggcctg agtctccacc
    70501 atatccctga tgttcgcaaa cgccagcttc atgttccctc cttgtcgtca ttcttccctc
    70561 acggatcggc gcggcgaccc gggattttcc ccggctataa gggcatcggg aaataaatgc
    70621 aatcaaaaaa acttttccca ttgatcttgt cccacatttt tgttatgcgt ttcgacgcgg
    70681 ggcggggttt tcgtcgcacc agggtccacc cgcagcacct gttgggccgg cgtgccccgc
    70741 aacgtcgtac acaacccttt cataagcaac cgccatcgca gtgtccatcg ccaaacggga
    70801 ggtattcatg aacatcatcg agaaggccct cggagacggt cgtcttgccc tttccgaata
    70861 cgaggccaaa caggtcctgt ccgcctacgg cattcccgtg acccgggaag tcctggtcga
    70921 aaaggaggtg gacctgaccg acgcgctccg gcaggtcggc taccccgtgg tcatgaaggg
    70981 gtgcgcggcc gccatcgccc acaagacgga aaagggcctc atccacgtgg atgtccgcaa
    71041 cgaagcggag gcgcgccaag ccttctccac ccttcgggag gccctcgcgg aaaccggggg
    71101 cgcggtcctc gttcagacct tcatccgggg ccagcgggaa ctcgtcgtcg ggatgacgcg
    71161 cgatccccag ttcggcccct gcgtcatgtt cggactcggg ggaatcttca cggaaatcct
    71221 ccgggacgtt tccttccgga aggccccgct cgaacgcgcc gacgccttgg agatgatgca
    71281 ggaaatcaag ggacacaaga ttctgggtgc cctgaggggc atgccggcgg tggacctcga
    71341 ggcgatggcg gagatcctga tccgcgtcgg ccggatcggt cttgaaaacg accgggttaa
    71401 ggaaatcgac atcaacccta tcatcattgt acaggggaaa cccatcgccg tggatgccct
    71461 gatcgttctc gaacccgctc cctgaggagg cgaggccatg tcgacggaca gattttccat
    71521 gcgggaggcc gtccggttcg gttgggtcac gacgaaagat cattttctct ttttcgtcgg
    71581 gatcctgctg gcggcatttc tgatcgtcat cgtccccgaa ggcatcggga aggcattgag
    71641 cggtctggcg gatgaacagg cgccgggcct ggggctcgca ttcaagggga tcggcctcct
    71701 tttttctctc ctcagttcgg tcctctacct gatcctgcag atgggattca tccggatctc
    71761 tctcaatttc catgacgggt ctcccagcgg cttcgccgac ctcttctcgt cctggcgcct
    71821 gtttttcaaa tacctgctcg gggcgatcct gtacggcctg cgctgcttct ggcccgtctt
    71881 cctgctgggc atcgtcgccg ccatcctgat ccccagcctc aaggcgcagg ccgccccgtc
    71941 cacgaccctg gcggcggcca tcaccctcgg cgttctcttt ctcattcttc tgcccctgac
    72001 cgtcatctgg gccatcaagt accagttttt cggctacctt gtcgtcgatc ggggcttggg
    72061 gccggtggag gccatccgga agagcgccga cgtaaccgac ggcgccaagg tggatctctt
    72121 cctcttttgc ctgctcatcg tcgggatcaa cctcctgggg atgctggccc tgatggtcgg
    72181 ccttttcgtc accctgcccc tctcggtgat cgcctacgcc cacgtgtatc gccggctttc
    72241 gggagaggcg ccggtcgtgc agcggcttga agacgccgaa gcggagccag acgcggtagt
    72301 cccgctggat atcgatctgg gcgggggaga atcgcccgtc cccgcggctg tgatgaagcc
    72361 gtccgtccgg atcgtctcga agaccgcccc ggcatccgcc ggcgaagaga cccggacgaa
    72421 ggggcttttc ctgctgtttt accggaacga ctacgagggc gccatccagg aattcacgcg
    72481 cgccatcggg caggacgccc gctacgccgg agcctacttc agccggggaa tggcgtactt
    72541 caaactgggg gagttcaacc tcgccctgaa ggatttcaac gaggcgatcg acctgaaccg
    72601 ggactatgcg gaggaggccg gtgtgttcgt ccaacgcggg ctcgcccgcg aaaaactggg
    72661 agaccgctcc ggagcggttc aggacttgcg ggcggcggtg gcgctgggca accgggacgc
    72721 gcagcgctgg ctgaactgga tcatggaaca gggcgaggat ctttagccgg gaggcgcgtc
    72781 agcccccctt cagtgtcccg ggttttcgat ccgcggcttc atcgccagga tcaggaaaac
    72841 ggccaggatc aggaccgcca ggttgagctg ccacgcgtag gcgtaagagc ccagacggtc
    72901 gtagagggct ccacccagca ccggcccgat gctcccgccg attcccgcca cgaagaacgt
    72961 cagaataccg taggccgtcc ccaggacctg ccggccgaaa cgatccgcca gcagatacgg
    73021 catcatggtg gcgatggacc cgtaaccgaa gccgaagagc agggcgtaga ccatgagcag
    73081 ggtcgcggac gtggcttgga gcaggacaat catccccgcg gccatgcaga tgaagcccag
    73141 cgaggcggag tatttcgcgt cggggatatg atcactcaac cagccgaaga agaaccgccc
    73201 cccgatgctg ccgatgccga tgaatcccag ggaggcggcg gcgaccacct cttcgatgtg
    73261 gttgtcccgg gcataggcga cctgatgcac gaaggcggac atctccgcca tcaccgccag
    73321 actgtagcag gcggcgagaa tccagaaccg ggagtccttc aggacccatc cgaccggtgc
    73381 ggcgttcacc gtcgctccgg cggaaaccgg aggcgcgccg gagcggtcgc cgtccggcgc
    73441 caggccgtag tcctcgggcc tggttttccc catgagccct tgggagagca ggacgccgat
    73501 cacgagaatc aggaccccga ggaacgaaaa ccccatccgc cagccataga ccttcacgat
    73561 atagccggaa agaggcgcca ggagcatcgt gccgatcccg atcccgatcg tagcgatgcc
    73621 gattgccgtt ccccgtcttt tcacgaacca cttgcccaca taggagctgt tgacgacgac
    73681 gccgaagaac gccgcgccgc cgccgcaaat cagcccgtaa acgagataga actgccaggg
    73741 tgcgcggatg tagcccatgg cgatgaatcc cgcagacacc acgagtgctc cgagggtcat
    73801 gagccacttc ggggccatcc ggtccaatag ccgaccggaa aggattccgc cgatgccata
    73861 ggtcaggatc atcagggaag cgcccacgga aatcaccgaa cgcgaccagc cgtactccag
    73921 ggccatgggc ttgacgaaga cgccgaaaca gtaccgcgcc ccataattga tccccaaaat
    73981 caggaatgcg cccatcacca cgtaccatcc ccagtacagc cccgcttcac gcttcatcgc
    74041 tcactccttc gcccggcgaa aagtttgcgc acgtgtatga tgaatccccc cgtttgtcaa
    74101 cgaggaacgg tccttatttc ttttccaaaa ttgcgcaatt gtgatacaaa gcctgaagaa
    74161 gaaaatccca tcgccgtctg cggacggatg acgccggggg agggcaagcc ctcccccggc
    74221 ggatcgcttc cgctttgggc gtcggaaagg acacgtcccg ccacagtgat caggaaatcc
    74281 tcccctccct ccgccagacg gatgagaaaa tcccgctctc cccgacggga gcccgccgcg
    74341 ggacaccggg tcatcgaggc ggacccgctc ctcaagacgg tgttccagcg gatcggcaag
    74401 cctctggaag cccccctcgt gccggatccg ttccagtgcc gggccgtcga agcgatccgc
    74461 catacggact gtctggtcac cgcccctacc ggttccggca agacctggat cgccgagcag
    74521 gccatccggg aggtctttct gcgtggtgga cgcgcgtggt acacctctcc cctcaaggcc
    74581 ctgaccaact ccaagtgggt ggagttcgga aagatcttcg gtgcggacca tgtcggcatc
    74641 gtcacgggcg acaccaagga gaacacggat gcccccatcc tggtcggaac gacggagatt
    74701 ctgagaaatc agctctacga cgccatgcat tccggtgaag atctgggctg cgagctcgtg
    74761 attctggacg aggcccactt cctgggtgat cgggaccggg gcgtcgtctg ggaggagatc
    74821 atgatctatc tccccgcccg catccatctc ctcctcctgt cggccaccat cagcaacggg
    74881 gaggagatcg ccgcctggct ggaaaagatc cggggcaagg tctgtaccgt ggtgaacgaa
    74941 agcaggcgcc ccgtccccct gtatccgctg tttctccacc cgaaaggccg catcataccc
    75001 cttttggcca aggagaagct ctacggccag gtccacgaat atctgcaccg ccctcacggg
    75061 gccggacacc gtcactcccg gccggacttc ggacatatcc tggaggtcct caagacgttc
    75121 aacctgaccc cggccatctt tttcctcaag tcccgttcgg aatgcgatgc ggccctcaac
    75181 tcctgccgga ggcaagcccc tgccgccgtg tccgatccct ttcccctcgt ccgggacgaa
    75241 ctcatggacc gcttccccta cctcgcgaac cacaagcaac tcgcgcatct cgatggtgcc
    75301 agggtggcgg ctcaccatgg cggccagctc ccggcctgga aatttctcgt agagactctg
    75361 atgaagcagg gctgtctcga cgcgattttc gcgacctcga ccgtcgctgc cggcgtcaat
    75421 tttcccgcgc ggaccatcgt cctgttcaat tcggacctgt tcgacggcca ggccttcagc
    75481 cccatgaacg gcacagaatt tcatcagatg acgggacggg ccggccgccg cggaaaggac
    75541 aagatcgggt tcctccttgc catacccggc cgcttcatgg atctgacgca tgtccgggac
    75601 ctgctcttca aagaggcgga gtccgttcac agccgcatcc ggagcgactt ttccatggtc
    75661 ctgaacctgc tgctttccca gacaccgcag gacatccggg agatattcgc cagctccttc
    75721 tcaaccttcc agcaaaagcg cggccctcag tccggcgccg cccttgccgc ggcggaacgg
    75781 tcccgcgccg gacggcgcac tgccccgatg cagactgcaa cggcggatct ctgggaagat
    75841 tttctccgcc acttggcgtt tctgagggcc gagggttttg tcgacacgga cgatctcttg
    75901 acggaaagcg gtgtctgggc ctcgaagctc cggctggatc aaccgctgct cattgcggaa
    75961 tgtctccggc ggggtgcctt cccccgcgag gacgccaaac tcctcgccgc ggtggtggcg
    76021 cccttcgttt acgatggcga ccaggacccg ttcccggaaa aagagcgtct gccgcgccgg
    76081 ctggttgcgg cctgcaaccg cgtggctagg gcgctgcaac cgtttgcggc gcgccaggag
    76141 gcagccggtt tcgacaccca cccgctctgc ttcggggcgg cctccgtcat ctacgattgg
    76201 gcccgcggtt ccgactggga cggcatcatc gaccgaaccg gcatcggcga gggagatctg
    76261 gccatgctcg ttctcaggac cgcagacaat cttcgccaat tggcctcctt gaacgaaacg
    76321 catccggaaa tggccgccct ggcgtggaat gcacgggagg ccatcctgaa ggaaccggtg
    76381 cggtttacgt aaattctgct tgacattccg agggataaac atctatatag gccgaccttt
    76441 gacgcagccc gtcctcggat ggctcatcca cgtccccatc gtctagtggc ctaggacacc
    76501 ggcctttcac gccggtaacc ggggttcgac tccccgtggg gacgccaatc atattagcca
    76561 gttaaggaat tcttcaactg gtttttttct ttggtggtac tttcggtggc actttcggtc
    76621 ggaattacat caagaaggct catcacacct gccaaagtgt gagtcaaggt gggggtgaga
    76681 ggatcggaca tgctggatat tgaaacggcg cgtgtggtgg gaaagggatt gcatcgtgtc
    76741 tgttacgccc atccacagga tgagaatctt tgcatcaagg tccttcttcc attggaatcc
    76801 aaggcacgtt tgatcgaggc ggtccgtgaa gtcggctact atcgtttact cgaaagaaga
    76861 ggcattccct ggaccatgtt gccgaaattc catggagagg tgcttaccag ccgcggtccg
    76921 ggatatgttt ttgacctgat ccgcgattac gacggcggcg tatcgcaaac actcggccat
    76981 tacctggcct cgtccctgga aacggaaagg catgccgaag aactggcgat gtccttcgat
    77041 ctcctgaggg attatttgct gcagtggaag attattacca tgacgatcaa ggccaaaaat
    77101 attctttacc atagaatcgg caaaaatgag ggaaggctgg tcatcgtgga taatatcggc
    77161 aattccgatt ttatccctat ctgtaattac gtcgattttc tggcggaaag aaaaatccgc
    77221 cgtaaatggc gccggtttaa ggcggcgtta cggaaagaat accccaaaaa cacgttcgtg
    77281 cagcggagat tcaatcccta atttgatcga ggaatagata ttgggacatc ctttttctgc
    77341 atcggcgggt gtgccaccca ccgttctatc cgctttttcc acttcagggc attccagaat
    77401 tccggatgaa acagcgggga ttttcagcaa gacaatcacc gctacaggcc agggctctac
    77461 cccatcattc cggcagatct ttcgatactg actcgcctca gagtcctttc agcgtatttt
    77521 cacggtttct caggtttcga aacacactgg aggtttcatg gatgaactgg aaagactgcg
    77581 ttctcatatt accgccgctg atttcaagaa aggggcaagg ctgggtgtcc tttacaccaa
    77641 attggcttat aacgatcaca cagggtcaca gcggggaaca cccggaactg aatgaatcca
    77701 gataatcaca ataaaacagg tggcttagac atcttttagt ttctcatttc acgccggtaa
    77761 ccggggttcg actccccgtg gggacgtcaa tcataaaacc catccgtcaa agaaggtttc
    77821 ccggcccgtt cctccttccg agagacgaat ttccagcctg cagacataaa agggtgcctg
    77881 ccctgcccga ttcaccccct tcccttccgg gcaaccctgt cccggccccc cagcctcgtt
    77941 ttgccttgac atcgcggatc agggtggtat gcatcttaca ccatcgctgc tctggttccc
    78001 tctgttccca cgaggggaaa acgggcacaa acaccggcag gcaacctccc gcaactccga
    78061 cacccgcgca gacggcaagg gtgggggcgg ggaatgaatc cgcaggggtg actccatggc
    78121 gttcagaatg gctctggcga agcggagggg gtttgtcgag atattttcct ggaaggacaa
    78181 cctcgccacc cggctcaccc tcatcgcact ttttttcttg ggtctctata tcagcgccca
    78241 gtggggcctc tgggcctccg tcgcgtacgt ccttctatgg gtggcatcct atcttgtcct
    78301 ctttgcaggc acgtgccgac gatgcgccta ttacgggagg tcctgcccca tcccgctgga
    78361 aggctccatc gtctgccgct tattcccgaa gagcgaacag ccgttcacgg ggtctgccat
    78421 cttatacgcc tcgatcgcct atgtactccg ggtcggactc cccgtcggga tcatccttca
    78481 ggaccggctg gttttcgagg ggatcctgtt gggtctgatc ttcatcctgt tttgggtttc
    78541 caatctgttt tggtcgggct gccccaactg catcaatgac ctgtgcccgc tgaacccggg
    78601 ccagggcaaa taagccgatg gccgctgacc gagaccggat tgccgaaacc atgacgtgga
    78661 ggttgtatga gccagcaaca tgaaactctc ctccaggata cgctgaagaa ggcgcgtcag
    78721 cgtatgcacc tctacacctg agcggaagca agcctgcagg gactgcaggc cctctgggaa
    78781 ttgcctgtgg cagcgtcgtg ggcaacggcc gggtacatgg gagccatcct ggccggcaag
    78841 acaacctgcg gcacgctgat cggcagcagc atcgccatcg gcctttactg cggccgtcac
    78901 gtcggggcgc cgcccgaggc gcatcccgaa cagagaagcc gttccatcga aggcgttaag
    78961 cagctctatg ccgatttcat caaaaccttc ggcgccaccg actgccttgc cctttgctat
    79021 tgcgatttca gcaatcccgg cgatgtgacc cgctatattg aaaaccgggc ctggcgggaa
    79081 tcctgtgacc gttttcttga tttctgcatt cagaaatgtc ttcaaatgag ccatgatggg
    79141 gtgatttgat ctggaggtgc cgcccatccc cgcatcgcca ggcgccctct ttatgtgctt
    79201 gacaggcccg tttcggcatg ttagtgaata cggaaaagga ttgccgtttg cctatgaacc
    79261 ccttatcgaa cagcgaagac gtcgagcgta aaatcctcct catcctccgc attctcaagg
    79321 aatcgccggg cccgataggg gcgcgcctca ttgcgcgccg catgcaggat ttcggcatcc
    79381 tcctgagcga gcgagccgtc cggtaccacc tcaagctcat ggacgagcgc ggcctcaccc
    79441 gacttgtcgg gcgacgcgac gggcgtttga tcacggagat gggcatcgat gaggcggccg
    79501 atgcccgcgt ccgggacaag atcggcctgg ccatctcccg gatcgaatcg ctggccttcc
    79561 ggacgacgtt caacccccag accatgaacg gcgtcctgcc cgtcaatgtc tcctttttcc
    79621 cccgggacca gttcgatacg gccttggccg ccatggcgcc ggccttcagg cgcggcctcg
    79681 cggtcagcga cctcgtggcc gtcgccgacg aggggcagcg gctgggcgag gtgcgggtcc
    79741 ctccggaccg catcgggttc gcaacggtct gcagcatcgt catcaacggc gtgctcctga
    79801 aaaacggcat cccgatggac tccaagttcg ggggcattct ccaggtcaag aacggccggg
    79861 ccctgcggtt cgtggagctg atctattact ccggctcgtc cctcgatcct tcggaggctt
    79921 tcatccttgg ccggatgacc tccgtaaagc gggtcatcga gacgggcgag ggaaagatcc
    79981 tggccaactt ccgggagatt cccgcgctga gccgggggct ggccgaggaa ctcttcctga
    80041 aactcaagcg ggccgggatt cggggcgacc tgtcactcgg ggagataagc gaacccgtgg
    80101 gccagacgcc cgtcgatgtc aacaaggtcg ggattatcct ggtcggtggc ctgaaccccg
    80161 tcgcctgcgc ccacgaactc gggctgatga cggaaagcca ggccatgtcc accgtgctgg
    80221 acttcaacga tctccagaat ttctggaccc tcctggacca tcggaatatc aaatacgacg
    80281 gataagacgg atcgtcccgg cacttcgacg attcgaaaac ttaattcatt ttttgaggag
    80341 gaaaattgta tggcagaatt gaatcccttt gtaatggcgc agaggcagtt ggatcaatgt
    80401 gccaaaatcc tgaacctgga ccccggcatc caggccatgc tccgggtccc cgtccgtgaa
    80461 ttgcacgtct ccctgccggt ccgcatggac gacggcgagg taaaggtctt tcaggggttc
    80521 cgcgttcagt ataacgacgc caatggcccg acaaagggcg gcatccggtt ccacccggac
    80581 gaaacgatcg acaccgtcaa ggccctcgcc gcctggatga cctggaaatg ctccctcgtc
    80641 gacctgccgc tgggcggcgg caagggcggc gtcatctgca atcccaagga gatgtcgcag
    80701 ggcgaattgg aacgcctgag ccgcgcctat atcgaccagg tctggcggat catcggcccg
    80761 gaaaaggaca tccccgctcc ggatgtgtac accaatcccc agatcatggc ttggatgatg
    80821 gatgaatact ccaagctctg cggcaagaac cagttcggcg tgatcacggg caagcccctg
    80881 gcgctcggcg gttccgccgg ccgcggcgac gccaccgccc ggggtggtct gtataccgtg
    80941 cgcgaggcgg ccaaggtcct ggggctcgac ctcgccaagg cgaccatcgc cgtccaaggc
    81001 tacggcaacg ccggatatta cgccgcctgc ctcgcccagt ccctgttcgg ctccaaaatc
    81061 gtggcgatca gcgactccaa gggcggcatc tgctgcgatg gcggattcga cccggtggcc
    81121 gtgggcgagc ataaggccca gacctcgagc gtctgcagct tccccgaaac ggatgccatc
    81181 tccaacgaag cgctcttgga aatggacgtg gacatcctct tcccctccgc catggagaac
    81241 gtcatcaccg agaccaacgc ccccaagatc aaggcgaaga tcatcgccga gctggccaac
    81301 ggaccgacga cgccggaagc cgatgagatc ctccacaaga acggcgtcca tgtcattccg
    81361 gacttcctgt gcaacgccgg aggcgtgacc gtgtcctact tcgaaatggt ccagaacttc
    81421 tacatgtatt actgggacga ggaggaggtg cacaagcgcc tggaaaagaa aatgaccgcc
    81481 gcgtatcaca aggtctatga gacatcgaag aaatacaaca tcaacatgcg gcaggcggcg
    81541 tacgtcgtcg cggtcgagcg cgtggtggag gccatgaagc tccgcggctg ggtctgaaaa
    81601 ccgccgccgc ttcgagaaac cttcatctca tcccaagggg ggataaaccg tccccccttt
    81661 tttcgtgcag gaagatcttt gagaatcggg atgcgtccgg gatcgagccg acgacactcc
    81721 cgacgccagg cgggttttgc gcttgacgcc ccggacggaa acggtgtaac ccagacccat
    81781 caaccggcgg ctccgcatca ccggcaacgc attgcgcccg acgcttcggt cccggcccgt
    81841 gtcaacggcc ggagcaccca gggcggcaac gtcccgggac gcgggcgccc cgcaagcgag
    81901 gcaacatatg tccccagtct gtccagaatc cccactcaga gcgggagacg tccgtcacgg
    81961 gtttctcgtc cagcgaatcg aagccatccc ggacctctgc gtcaccgcct gcgagctcgt
    82021 ccacgaggcc accggcgccc aggttgtcca cctccaggcc cacgaccggg aaaacctctt
    82081 cgcgatcggt ttccggaccc cgcccccgga ttccacgggg ctcccccaca tcctggagca
    82141 ttctgtcctc gccggctccg agcgctatcc ggtgaaggac gctttcaacg aactcctgcg
    82201 cggcacgctt cagacgtttc tcaatgcctt cacgtatccg gacaagaccg tctatccggt
    82261 cgccagccag acccggcagg acttctacaa cctggcccgt gtttacatcg atctcaccct
    82321 ccggccccgg ctgcggcggg agaccttcct gcaggagggg caccacctgg aattcaccgt
    82381 gccggacgac atcgcgagcg acctgaccgt ctcgggggtg gtctacaatg aaatgaaggg
    82441 ggcctattca tcgccggatg ccctgatggc caaggtcatc caggagtcgc tcttcccgca
    82501 aacgccctac gcctgcgatt ccggagggga cccggacgtc atccccacgc tgacctacga
    82561 ggccttccgg gcctttcacc ggatgtatta ttcgccctcc aatgcccgta tcttcctcta
    82621 tggagacatc cccacggagg atcatctgat ctttctcgcc gagatgctgt ccggtttcgc
    82681 gcgtgtatcg gtcgattccg caatccccgg gcagcctcgc tggaagaagt ccgtcgccgt
    82741 cagaggcacc taccccatcg gccgcgacga atccgcctcc ggcaagagcg tcgtgaacgt
    82801 ggcctggatg ctggcggaga atctcgacgg ggaaacggta acgctcctgg aagtcgccgc
    82861 agacgccctc gtgggcagcg ccgcgggtcc cctccgcaag gccctgatcg attccggtct
    82921 cggcgaggat ctctcgtcgg ccacagggat ggaacgggat ctgcggcagg tcgcctttgc
    82981 cgtcggtctc cggggtacgg acgccgaccg ggccgaccgg atcgaggccc tgatcctcga
    83041 caccctcgaa cgcgtggccc gcgagggctt cgcgcgcgac ctcatagaag gcgccctcca
    83101 tcaggtggaa tttcacggcc gggagatcgt ccggggcgcc tacccctacg gcatcgttct
    83161 gatggggaga gcctatcaca cctggctgta cgacggcgat cccttcgccg gcctcaactt
    83221 cccccgcatc atcgggcgta tccgggaaca atggtcgggc cggtccaccc tgttccagga
    83281 catcgtccgg cagtggctca tcgaaaatcc ccaccgggtg ctctccgtca tggtgccgga
    83341 cccggacgat ctgccccgcc gggaggcggc cttccgagag cggatggccc gcctgaaggc
    83401 ctccctgccg gtcgcgcggc tggaggaaat ccgaacccag accgaggcac tgcgaaaata
    83461 tcagaccgaa gcggattctt cggaggcctt ggccacgctg ccgaagctgc agctcaaaga
    83521 catcccgcgc gacgtggaaa ccgttcccac gcagaggacg gcgatcgccg gtgttcccac
    83581 cctgcggcac gaactgttca ccaacggcat tgcctatctg gacctggcct tcgacctctc
    83641 ccacctcccg gaggcactcc atccttggct gccgctcctg ggcaagtgca cgacccacat
    83701 gggggcggcc ggacacgact atgcggacat ggcccggcgg atcaccctga agacgggagg
    83761 gctcggctgc ggcctgcagg cggggctcct ggcggacgac cggggccact ggcagaagat
    83821 gacgtttcac gtcagggccc tgcaccgcaa tatcgaggag gccgtctcga tcctgatcga
    83881 cctgctgtcg gccggtgatc tggaagacgc cgcacggctg aaggacctga tcgccgagcg
    83941 gaaaaacggc ctccaggctt ccgtcatccc ttccggccac atcttcgccc gccgggcggc
    84001 gggggcggcg ctttccctgc cggcctaccg ggacgagcag tggcacgggc ggacgcaact
    84061 caagtttatg catgcaaagg cgcaacaggg gaacgccgat ccgaccgcgc ttcaagagcc
    84121 cctgcgcaca cttaagtcga cgatcttcaa acgcgcgggt ctgatcctca atctgaccgc
    84181 cgacgcggca ggtctggacg gcctcacggc ggccctggcg ccccggctcg gcgccctccc
    84241 ggcgggggaa gacacggcgc ccgccgcacc cgtcctgcca ctcccgcatc atccgggcat
    84301 cgcgatcgcc gcgcaggtct gttacgtggc caagctcctc cccgcccccg cctacgacaa
    84361 tcccctgacc gccgccctga tggtcgccgc ccggcacctc gccggcggct atctctacaa
    84421 gcacgtccgg ctccagggcg gggcctacgg aggcctgtcc gcctatgatc ccctggccgg
    84481 cgtgttcgcc tttctctcct accgggaccc ccatatcgtc cagaccctca cggcctatac
    84541 gggtgccgtg aaaaccctga tcgacgcacc ggtcccggtc gaggaactga ccaaggccat
    84601 catcggcacc atcggcgccc tggacaaacc cctggaccca tccggacgcg gccacgtggc
    84661 catgatccgg gagttcgtcg gattgacgga cgaacggcgg caacgcctgc ggagcgccat
    84721 cctggagatg acgccgcagg gtattcggga agcaatggcg cggtacttcc gggaggccga
    84781 gccgcgggcc gatgtcgccg ttctggcgcc ggaggatcgc ctccggcagg ccaatcaggc
    84841 cctcgggaac acgctggtgc tggagacgct gggctgaagg agcgtggcgg ggcaacgggt
    84901 gtttccagac ggggcgggag acggacggaa ccgacccgac agggcgggga agggctcgcc
    84961 cggactactc cgtccggtaa gagaccagac cgaccatcgc cagaaaggcg atcacgaggc
    85021 cgagtccgaa ataggtgaag gtaacggtgg ggaccaattg accccagacc cgatgaggag
    85081 gaaaaccgcc atgaaacgaa cgacagccct gagcatccgg ttcgtcataa ccggtctgat
    85141 cctgctcgca atcggctgtg ccggatggcc ggcagcccag caggcgggcc gattcgacct
    85201 ggatgagggc gtcaccgggg cgttcgaacg ctacgaaatg aaccccggat acgactacta
    85261 catcagcggc tccgacgcct gccccaacgc ggtactgggc ttggaaaaag ggatatctct
    85321 gggcgaaacc ctctggaagc cgtttcccgc cgatcccgcg gccttcaagg acatcatcga
    85381 ccgcatgagg ctccgggcga tcaatctgca ccgccaccag tacggcttcg tcctccggga
    85441 cggcgcaggg caggcgattg gcgtctggta ctcgctgatt tccgcgcgaa ccgtcatccg
    85501 gcgccggagc gacggcacct tcatgatcta cacgccggat ctggacacct acgacctata
    85561 cgaacgggag gactgaactg cgccgaggaa tggctctctc ctcacaaaat ctcctcgatc
    85621 ttgttgagcg ccgccggcag gttttccggc cggggccctc ccgcctgggc catgtccgac
    85681 cggccgccgc cgctgccgcc cacgaccggg gcgagggcct tgatgatccg gcccgcattg
    85741 tagcgaccgg tcagatcctt cgtcaccagg cacaggagca tcgccttgtc gccggaccga
    85801 ctgcccaaga ggatgatccc cgaaccgatc cggtccttca gtttgtcacc gaaatccctc
    85861 agggtcttga catccgcgat ctccacctgg gccgtcagga cgcgaacccc tttgacctca
    85921 cgcgcctgat cgatcaggtc ggccgaatcc ttcgccgcca gttttccctt cagggcctcg
    85981 atttccttct ccagctcccg ctgctgcttc aggagttttt ccatcctttc ggacacctcg
    86041 aagggcgagg ccttcacgaa accggccgcc ttcttcaatt cttcctctag acggcgggca
    86101 tacaccaccg caccccggcc ggtcacggcc tcgatccgcc ggacgccggc ggcgacggcc
    86161 gattcgtgga caattttgag aaacccgatg tcgcccgtcc gcgtcacatg ggtcccgccg
    86221 cacagctccg cgctgacctc ccccatccgg acgacgcgca cctccgcccc gtacttctcg
    86281 tcgaagaccg ccgtggcgcc ggatttcatc gcctcttccc gcggcaggag cttggtcacc
    86341 accgggagat tccgccggat ggtttcgttg gcgagggctt cgatgcacgc cagctcgtcg
    86401 tcctcgatcc gggagaagtg ggtgaagtcg aaacgcaggc gttccggatt gacgagcgat
    86461 ccggactgct tgacgtggtc gcccaggacc gccttcaggg cggcgttcaa cacatgggtg
    86521 cccgaatggt tcgcctcggt cgcccgccgg tcgcccgcct ccaccttcag gtgaacggca
    86581 tctcccacgc ggagacgccc gcgtttgagc ttgccgatat gggtgacgat cccctccacg
    86641 ggacgctggg tgtcccaaac ctcgaactgg acgccgtcac ccgtgacaac tcccgtgtcg
    86701 cccacctgac cgcccgtttc gccgtaaaaa ggcgtctcgg ccacgaagag ttcggcgctg
    86761 tcgcccgccg cgatctcctc cacgggccgg tccttcttca ggagcgcggt cacccgggag
    86821 tcggcctccg tccggccctc atagcccaca aaggtggtgg cgacgcccgc cacggacagt
    86881 tgctggtagg cctccgcaac ggcctcctca ccgctgccct tccaggattc ccgcgccttc
    86941 tcgcgctggg cctccatggc ccgttcgaaa ccgtccatat ccaggcgaat cccctccttc
    87001 cgaacgatgt ccgccgtcag gtcggtggga aagccgaagg tgtcgtagag cttgaaaacg
    87061 atctcgccgc caagaacccc ctggccgccc gcctggagac gcgccacctc ctcctggagg
    87121 attttgagcc ccgcgtccag ggtatcgctg aaccgctgct cctcgttgac cacgaccttg
    87181 tcgatatagg agcgcttgtc cgtcagatcc ggatagaccc cctgcatgag gtcgatcacg
    87241 acgccgcaca cctggtggag gaagggctta tcgaacccga gcagtttccc gtggcgggcg
    87301 gctcgccgca ggattcgcct caggacgtag ccccgccctt cgttgctcgg cagcacgccg
    87361 tcaccgacga gaaacgcgac ggcccggcta tggtcggcaa tgacccggat ggagacgtcc
    87421 gactccgcgc ccgtcccgta cgccttgccg ctcaggcgct cgatgaagcg gataatcggc
    87481 cggaaaagat cggaatcgta gttgctctta accccctgga caacggcggc cagccgctcc
    87541 agacccatgc ccgtgtcgat gttcggattc ggcagggggc tgagcgtccc ggcggcgtcg
    87601 cggtcgaact gggtgaagac gaggttccag atctcgaggt ggcggtcaca ttcgcagtgg
    87661 atgtcgcatt ccggtcggcc gcagcccgtc ccctcgccct ggtcgtagag gatctccgaa
    87721 caggggccac agggacccgt ttcgcccatc atccaaaaat tgctcttctc gcccatgcgg
    87781 acgatccgct ccgccggcac gcccatctcg tcgcgccaga tcgcataggc ctcatcgtcg
    87841 tccttgaaaa tcgtaatcca cagcttgtcc ttcggcaggg ccatgacccc ggtcaggtac
    87901 tcccaccccc aggcgatggc gtcctttttg aagtagtcgc caaaggagaa gtttcccagc
    87961 atctcgaaga aggtgtggtg ccgggccgtt acgccgacgt tttccaggtc gttgtgcttg
    88021 cccccggccc ggacgcactt ctgggagctc gccgcccgct tgtagccccg gtcttcgagg
    88081 ccgagaaaac agttcttgaa ctggaccatc cccgcattgg taaacaggag ggtcggatcg
    88141 tccttgggaa cgagcgatga gctggcaacc cgcgcgtgac ctttcccctc gaaatacctt
    88201 agaaaactct ccctgatctc cgtccccgtc atcatgtgaa acaaccgcct cctgtacaaa
    88261 atagtctccg gccaaaggtc cggggtgaac gccgaaaggc tccgttcctc cgaacctttc
    88321 gccctcggcc ctcagccttt cacgcttcgc ccttcgcctg cattcaactc cctcacgatc
    88381 agatccaacg ggaacccgcg cccggcaagg ctttgggcta gacgccgctt ttcccggagc
    88441 tccagcgccg cgggatcgcg cccccgtagt ttcctttcca ggcaccgccg cagggcctcg
    88501 ccctcgtcca cgtcctcacg gatctcggac agcgtcgtcc ggatgagctc cgccgggatt
    88561 cccttctccc gaagccccgc cgcaatccgg cgatcccccc agagccgccc gcccgcccgt
    88621 cctttcgccc acccccgggc aaaggcggcg tcatcgacgt accccagttc ccggagccga
    88681 tccagcaccc gggcgatgcc cgctccggaa gccccctttt ccttgagctt ccgctggatc
    88741 tcccgctcgc tgtgggggcg tctggccagc atccggaagg cccgctccag ggcctcctga
    88801 tcggagaggg tgtccgccac ggcgctacgc ctcctgctcc ccctttttga gtccgaactt
    88861 ctcccggacc tccacctcga tccgggccat cacctcggtg ttctccttca ggaagagctt
    88921 tgtattgtcc cggccctggc cgatccgatc ccccttgtag gaataccagg ccccactctt
    88981 ctccacgatg ccctgctccg ccgcgaggtc caggatgtct ccctcccggg agatgccctc
    89041 gccgaagatg atgtcgaatt ccgtctcccg gaagggcggg gcgaccttgt tcttcacgac
    89101 cttgacgcgg gtccgcatcc cgatcacgtc ctggccggat ttgatcgtgc tgaccttgcg
    89161 aatgtccagg cgcatggacg cgtagaactt gagcgcgttg ccgccggtgg tcgtctctgg
    89221 gttcccgaag aaaacgccga tcttcatccg gagctggttg atgaagatca cggtcgtccg
    89281 cgacttgctg atgctgcccg tgagcttccg gagggcctgg gacatcagcc gggcctgaag
    89341 gcccatctgg gcgtccccca tctccccctc gagctccgcc ttcggcacca gggcggccac
    89401 ggaatcgacc accagcacgt ccagggcgcc gctccgcacc agcgtctcgg cgatctccag
    89461 ggcctgctcc cccgtatcgg gctgggagat caggaggtcg tccgtgtcga cgccgatctt
    89521 cttggcgtaa ccgacatcca gggcgtgctc ggcgtcgatg aaggccgcga gtcccccctg
    89581 cttctgggcc tccgcgacca tgtgcagggc gagcgtcgtc ttcccgcccg attcggggcc
    89641 gaagatttcg atcacccgtc cccgcggcac gccgcctacg cccaaggcca gatcaaggct
    89701 caacgatccg gtggaaatcg ccggcacgtc ggccactctt tcaccgccgc cgagccgcat
    89761 gatggacccc ttgccgaact gccgttcgat ctgggtaatg gccagatcca ccgctttcat
    89821 cctctccgta tccgcgacca tagccacctc ctgataatga tcttttctct tcaacattcg
    89881 tgggtatgcc tcttcctttt cccgcttttg ctcaagcgcg attctggtaa atcctcattt
    89941 ctccgctctc cgcgcgtcgg accccctgag gcccggcgcc ccggcgggag ccgacccatt
    90001 cccgcccctc gcctcgtttc cggaaggggc gggtcatccc cggaggggaa aggcggcaag
    90061 ccgggcatac acggcgccct gcggcgtcaa ttcactccgg aacaggacca ggtgatcggc
    90121 cctgaacaga ccggccgtcg ccctctcgcc gatctcgacc gcgcgggcca ggccgaccag
    90181 gcccttcggg gtcttgatcc gccccagggt caggtgcgcc cggaaaggcc gctcctcccg
    90241 cggaaacccc aggaccccga acgcccgttc gagcgacgtt tgcaggacga gcagacgctc
    90301 cacctcccct gccatcccca gccagaccac gcgagggcgg ttcagatcgg ggaaaacacc
    90361 gaccgtccgg accgtcagat cgaacggggc gaccccgacg gtccgtgccg cgaccgcctc
    90421 cgagatccgg tccacggccg gcggcgggat attcccgaag aacttgaggg tcaggtgaat
    90481 ctccgccgga cggacccacc ggatcacccc ccggaccgtt ttcttcagac gttcctggat
    90541 cgcggcgatc tctccgcgca cggccgccgg gggctcgacg gccaggaagg cccgcagggt
    90601 cgtttctccg ctcatcgctt ccccaaggcc gccctttccg tcacgcctgc tgcacctcac
    90661 ccgcgagata gcgcttcacc atcatgagcg cggactgcgc ggcgatcatc ttgatccggc
    90721 ggcggtccca gcggaaggcg aaccgccggc agaccgtttc atcggcgtcc gccagggcga
    90781 tgtagaccgt ccccacgggc ttcgccgccg ttccgccctc cggtccggcg atgccggtca
    90841 cggcgagacc cagatccgtc cccgcctgcc gcctcacccc cacggccatc cgccgggccg
    90901 tctcttcgct gacggcgccg tgcaccgcga ggacggcctc cggcaccccg aggagcgcca
    90961 ccttggcggc gttgctgtag gtcacgagtc cccgttccag gtaggcggaa ctgccgggaa
    91021 catccgtcag ccggtccgtg acgagcccac ccgtgcagga ctcggccacg gccagggtga
    91081 gcccccgttc actgagcagg cgccccacca cggcctcgag ggcttccccg tcccgcgaga
    91141 agacatggcg ccccaggcgc cccgtcaccg ccgcctccgc cgcctgcagc cgccgttcgg
    91201 cctccgcatc ggtcgccgcc cgcgccgtca ggacgatgtg gttctccggg aagtgggggt
    91261 agaagccgat ctcgacgccc attccggtcc agtcggcatc ggccagggcc cgatccacct
    91321 cggcctcgcc caggccgaaa ctcttgaccg tccgggtcgc aacacaggcc gccgcctcgg
    91381 gaaacgcccg cctcaggagc gggacgaccc cctcgggcag catccgtgca acctcccggg
    91441 gaacgcccgg aatcaccgcc accacctggt tgcccttctg gagggcaaac cccgccgccg
    91501 ttcccacggg gttgggtatg acctccgccc ccgccgggaa aacggcctgc ttggcgttgt
    91561 tgtccgtcca gttcagacgg tatttctcga agatcgactt gatggtggcc aggaccgcct
    91621 tatccgtcaa gaggggcagc gcgaagatcc gggcgatcgc ggcggtcgtg atgtcgtccg
    91681 ccgtgggccc cagtcccccg gtcacgacgg cggcgtcggc ctgcgccagg atcagcccaa
    91741 gggcctcccg gatcgccgcc tcgtcgtccc ccacggacag gatgacgacc accggccacc
    91801 cctggatctg gagggtccgg gcgatcagcg aggcgttcgt gtcctgcgtt cgcccgctga
    91861 tcagctcgtt gccgatggtc agaatgccga ccctcatgtt gagatatccc ccgtttaacc
    91921 cagaacgccc gcccacacca gggcccgcag gacgagattc gcgtagaccc ccgccgcgac
    91981 gtcgtccagc accaccccgt aaccgccggg aaaccgccgc tgaatgagac cggccggaaa
    92041 gggcttcaca atatcgaaga gccggaaaag cgcaaacccg gcggcgatat gcgtcacggt
    92101 cggcgtgacg cagaacatgg cgaacagaaa cccgcagatc tcgtcgatca cgatccgcgg
    92161 ggcatccttc cggccaaaga gaacctccgc cgccccggag acgtgaatcg ccagaaacgt
    92221 cagtgccacc gccgtcagga gatacagcgg ccaggacagg ggaaaaagga cgagataaag
    92281 gggaatcgcg acgagcgtcc ccgccgttcc gggggcgaag gggacatacc cgctccccag
    92341 accggtggcg atgatcctga caaacctctc gcccaagggc gccccttccg gcatcctgta
    92401 cggcgggatc cgccgggcgt aaaccgccaa aaatgccttc atttttccgc tctcagttat
    92461 cctcttttcc cccccctgtc aatgagcaaa aaaccccttg acaatagaac gtgcgttcta
    92521 tcataatgga tccacggagg caagagaaaa agattctccc ggccgtaaac cgcttgagat
    92581 ctctgaaaag cgcgatccgt ttcccaaagg tcaaggattt cgggcaatac gatgaacgac
    92641 gcgatcttga tgccccaccg ctggccccgg gccatcctgc acgtggacgg ggacgccttc
    92701 ttcacctcct gcgaggaggt cctccacccg gaattgcgcg ggaagcccat tgtcaccggc
    92761 ggcgagcggg gcatcgtcgc ctgcgcgagc tacgcggcca aggctctggg catccggcgg
    92821 gccgtttccc tcccggaggc gaaacggctc tgtccggacc tcgtcgtcct cccctccgac
    92881 tacgagacct acagcctcct ctcccggcgg atgttcaacg tcatgcgccg cttcaccccc
    92941 caggtcgagg aatactccat cgacgaggcc ttcgccgaca tcacgggcct caggcggccc
    93001 ctcaaggcct cctacgaaac catcgcactg cggatgaagg acgccatcga gcgggaactg
    93061 ggcctgaccg tctccgtcgg cctctccctg accaaggtcc tcgccaaggc ggcctccaag
    93121 caccggaagc cctccggttt caccgtcgtc cccagccggg ggatccccct cctgctcgag
    93181 tcgcttcccg tgggcggggt ctggggcatc ggccccgcga ccgcgagcta tctcgacaag
    93241 ctgggcgtcc ggaccgccct ggacttcgcc cgccttgcgg agcggacggc ccgtcagcgc
    93301 ctcacgaagc ccggcgtcga gacctggcag gagctccggg ggacgtccgt ctaccccgtc
    93361 tcgccggagg agaagagcgt ctatgcctcc atcagcaaga cgaagacctt cgccccgccg
    93421 agcgccgatc cggagtacct cttcgcccac ctggcccgga acctcgaatc cgcctgcatc
    93481 aaggcccggc gctacggcct cgccccgcgg cgggtggccg ccttcctgaa gcggcagaac
    93541 ttcgatacat cggggcggga ggcgcgcctc agccgcccct gcgcccaccc cctggagctg
    93601 tcccccctgc tccgcggcct gttcaacgac ctctaccggc gccgggacct ctaccgcgcc
    93661 accggggtcg tcctcctcga cctggccccg gacgcgctca tccagtactc cctcttcgaa
    93721 gaccccctga aggcggaaaa gatccgcgag ctctacgaag ccgtggacgc ggtgagccgc
    93781 cgctacggca agcacgccct ccacctgggg ggctcccacc tgatcgaggt ccggggcaag
    93841 ggccgccggg gcgacccgac ggtccgggag aacacctgcc cctacggcaa gacccgccgc
    93901 aagcagctgg ggctgccgct cttccggccg gcctccggga acgcggagac gtaacggccc
    93961 cctgcccccg atggtctttt actttttccc ggaaatctgc taggtttcct gcgctacggg
    94021 cgtttccgcc cgccatgggg aacgggacgc cccgaaccgc gcgaatgttc cctgtgcgaa
    94081 aggaccgcca tgaccggaac taagaaggtt ttctcccgtt tcatctggat cggcgcggct
    94141 cttctgctgg cggccgccgc cggttacgcc cttctccacc gccaggacca gaacaccgtc
    94201 ccgccgggat tcgtcgtcgg caacggccgt ctggaggcga cggagatcga catcgccacg
    94261 aaacagccgg gacgcttgat ggcggtcttt gtccgggagg gggacgccgt ccggccgtat
    94321 cagctcctcg cacggatgga taccgcgccg ctcgaggccc agctccggca ggcccaggcg
    94381 gaggtccggc gggccgaaga ggcccgccgc tcggcgctgg ccaaggtcga tgagagccag
    94441 agccgggtgg tcctcgcccg tcgggagctg gagcggtcgg agcggcttct ggccaaggga
    94501 atcgccaccc agcagcggct ggagcacgat cagaccaacc ggcagagcgc cgaggccgac
    94561 gatgccgcgg cccgggccag ggtggccgaa gccgaggccg cgatcgcggc ggccgtcgcc
    94621 cggacggatt acctgaaggc ggaaatcgcg gacgggtccc tctggagccc cgtccggggg
    94681 cgcgtgctcg tgcgcctcgc cgagccgggc gaggtgctgc cggccggggg caaggtcctg
    94741 acggtcatcg acccggagga gatgtacatg accgtctttc tgccggaaaa ggaggcgggg
    94801 aatctcgccc tgggcgctga ggcgcgggtc gtgcccgacg cctttccgga gcgccccctc
    94861 cgggcgcgcg tcgtcttcgt ctcggccaag gcccaattca cgccgaagga ggtggaaacg
    94921 gccgaggagc gtcagaagct cacgttccgg gtcaaggtcc gtctggagcc ccaccgtgaa
    94981 tcccgcctga aaccgggtct gccgggcgtt tcctgggtgc gcctcgatcc ccgggccccc
    95041 tggccccctt ccctgcaatg aacgaccgga cggatatccc gccccccgtc tgccgcatcg
    95101 ccggcctccg gcaccggtac ggcgcccggc gggccttgga cgacgtctcc ctggagattc
    95161 ccgcggggcg ccgcgtcggc ttcatcggtc ccgacggcgt cggcaagtcg acgatcctgg
    95221 gcatcctggc cggcctgaaa aagattcagc gcggaaacgt caccgtcctc ggcggcgaca
    95281 tgcgttccgc atcgtaccgg cttcaggtct gttcccggat tgcgttcatg ccgcaggggc
    95341 tgggacagaa tctttacacc accctgtcgg tccgggaaaa ttgcgatttc ttcggccgcc
    95401 tctacaattt ctcccgtccg gagcgggatg cgcgcatccg cgaactgctc tccgcgaccc
    95461 gcctggctcc cttcgccggc cggccggccg gcaagctgtc cgggggcatg aagcagaagc
    95521 tgggactgtg ctgcgccctg atccacgatc ccgatgttct cgtcatggac gaaccgacga
    95581 cgggggtgga ccccctgtcc cggcagcagt tctggaaact catcgacgag atgcggcttc
    95641 ggcatcgcgg catgagcatt ctcgtcgcca cggcctacat ggaagaggcg gagcacctcg
    95701 actggctggt catgatggcg gggggacgcg tgctggccgc gggaacaccg ggggagttaa
    95761 aggcgctcag cggcaaagag acgatcgagg aggcctacat cgccctgctc ccggaggaga
    95821 ggcgtgccgg tcatcgcgtc tttgccgtcc ccccccgcat cctctccgcg gatggcgcgg
    95881 ccatcgaggc ccagcacctc acaaagcgtt tcggggcctt caccgccgtc gaggacgtct
    95941 ccttcacgat cccgaaaggc gaaatcttcg gctttgtcgg ctccaacggc tgcggcaaga
    96001 cgaccacgat gaaaatggtg acggggcttc ttccccccac ggcgggcgag gcctacctgt
    96061 tcggcagcgc ggtcgacgcc ggcagcatcg ccatgcgcaa acgcatcggg tacatgtccc
    96121 agaccttctc cctgtacggc gagctgaccg tccggcagaa cctgaccctg cacgcccacc
    96181 tcttcgacct gcccgcggcc tcgatccccg cccgggtgga agaaatgagt gcggttttcg
    96241 atctgggcaa cgtcatggac gatcaggcgg agcgactgcc cctcggcatc cgccagcgtt
    96301 tgtccctcgc cgtggccgcg atccacgatc cggaagtcct gatcctggac gaacctacct
    96361 cgggggtgga ccccatcgcc cgggaccact tctgggaact cctggccgga ctttcccgcg
    96421 agcgccaggt gacgatcttc atcacgaccc actacatgaa cgaggccggc cgctgcgacc
    96481 gggtggccct gatgagccag gggcgcgtcc tggcctgcga tagcccgaag gaactcacgc
    96541 gtcgccggaa gtgccggacc ctggaagagg cgttcatcgc ctacatgcgg gaggatgccg
    96601 acggggacga ccgcgaagcg gacgcgggag gggccggcgg ccttgaacgt cccgccacga
    96661 tcttgacacc cccggcgcga ccggaatccc gacgccgctt cgatatccac cgccttttcg
    96721 ccgtggcgca caaagagacg ctcgaactcc tccgggactc ggtcctcctc ctcttcgccc
    96781 tcctcgtggc gccgctgctg ctgctggtgt atggctacgg catcaacacg gatatcgatg
    96841 cgattcgctt cgccgtcctc gaccatgacg gaacggcggc aagccgcgcg tatgtcgagc
    96901 attacaccgg ttcgccctac ttccgggatc gggggcaaat cctgtctccg ctggagatgg
    96961 agcggggatt tcagaagctg aattactccc tcgtcatcga aattccgccg gggttcgcca
    97021 gagacctgaa gcgggaacgc agccccgcgg tgggggtgtg gatcgacggg accatgccgt
    97081 tccgggcgga aacggcgaag aattacgcgg aggccgtcca tctggcctat ctggccgagc
    97141 aaccggaggc ggccgccctc ccggtcacgg gcggcgcggt acagatcgaa tcccgctact
    97201 ggtacaaccc caacgtccgg agccgcctgg ccatcgttcc gggcgttctg gccgccgtcc
    97261 tgctgctcgt gcccaccctg ctgacggcgg tggccgtggt gcgggaaaag gagctcggct
    97321 ccatcacgaa cctctacacc agcccggtca cgcgcctgga gtttctgtgg gggaagcagc
    97381 tcccctacgt cgtcgtcaat gcggtcaatt tcttcgcgct gatcgcgatc attctcgggg
    97441 tgtttcagat cccgttccgg ggcagcttcc cggccctcgt cgtcggggcc cttctctatc
    97501 tcgtcgccag cacgggactc gggctgctcg tctccacctt cacccggacg cagatcgcgg
    97561 cgttggtggc ggccttcgtc ctgacgatgg ttcccgcgtt ccagttttcc ggtctcctga
    97621 cgccggtctc gtcgctcacg ggcggcgccg ccttcatggc ccggaccttc cccgcccatt
    97681 actttctcga catcagcgtg gggaccttca ccaaggccat cggatttcag gaactggcgg
    97741 cccattatct tgtcctggca gcgatgtgcg tcgccctgac ggccacgacg gtcgcgctgc
    97801 tcaacaagca ggagaagtag cgatggaccg gatcgcccac atctaccggc tggggttgaa
    97861 ggagctcatc agcatccgcc acgaatacat cctgatcttt ttcctggtgt actcgttttc
    97921 cgccctgatc atcgttacgg cgaagggaac cgggcttcag gtgaacaacg cctccgtcgc
    97981 gatcgtcgac gaagaccgga gccccctttc ggcccgcatc cgggcgtcca ttcgcaaacc
    98041 ctattttcag acgccccggg acctgacctt cccggagatc ggcggaaccc tggcccgcaa
    98101 cgccgtgacc ttcatcatcg acatcccgcc cggtttccag acggacctcc tggccggacg
    98161 gcggcccgag atccggatcc atgtcgacgg cacggccatc ggtcatgcga agatgggaac
    98221 cacctacctg aagaaaatga tcgccgccga gatctcggcc tatctcctgg gcgaacggca
    98281 gacgccggcg cccgccgtgc agcagaccgt ccgcgtccgt tacaacccga accgccagaa
    98341 cacctggttc atgggactca tgatgctcgt caacatgatc accatgctct ccatcattct
    98401 tcccgcggcc gcgctgctcc gggaacggga gcacgggacg gtggaacacc tgctggtcat
    98461 gccgctgtcc ccgccggaga tcatgatcgc caaggtctgg gcgaacagcc tcgtcgtcct
    98521 gacaggcgcc attttctcac tgtggctcgt ggtccggggg gttttggggg cgccggtggc
    98581 gggctcgttc ccgctcttcc tcgccgggac ggtcgtctat cttttcgcca ccaccgggct
    98641 cggcatcctc ctcgccacca tcgcccggaa cggaccgcag ctgggactgc tctgcgtgcc
    98701 cgtcatcacg ccgatcacga tgctctccgg cgccatgacg ccgctcgagg ccatgccggc
    98761 gtttctacag accgtgatgt cggtctttcc cacgacgcac tacgtccaat tctcgtcggc
    98821 ggttctcatc cgcggggccg gaacggcgat cgtctgggaa gcgctcgcgg ccatggcgct
    98881 catcggcgcc gtcctctttc tcgcggcgct cttccgcttc cgggcaacct tccgctaacg
    98941 gtcgcctcga ccgttccgtc aagcgggcag agaaagcccc gcccggcgat tccagcgccg
    99001 ccaccgccgc cggtacagag gggcgcgggg cgcgggcttc agccggagcg ccgccacccg
    99061 gtcgtgccag gcccggtacg cctccaggac gcgcgtcagg cagtccgcgg aataggcgtc
    99121 gagcgcggcg gcggtcggag cgccgccatc gaacagggtc ctctccccgc ggacgaacgc
    99181 gtcctgcagc tccgcctgct cccgccggaa ggcctccacg gccgacggat agttcgtgag
    99241 gcacaaccgg tagagccgtt catggcgcca ccacagggag tcgtcggccc gcgccgcggg
    99301 catccgcccg ccggcatcca tcagggtcgt cccgggcagg gtcaggggaa ggaagacggc
    99361 cgtgctcggg atcgacgttc cggtcatcca gcaggtggac ggtccggcgg ggcgcatctc
    99421 cccgacgagc gaacccgccg tctgggaggg cgtgaacaga ccggtggcgt gcatgcagat
    99481 cgaccccatg tccgagcggg ccgggtggaa cggccccgtc cggtccgggt cgccctccat
    99541 gcggaggatg tccatcgcca tcgccgccgt caggcgcccg cgcgccgcca ggcccgcccg
    99601 cgtggttcgt tccctccgga ggcgcgagcc gctgagatgg gtgtaaaaga catcgctgaa
    99661 gcatccgcgg aagctgaagg tcgcgccccg cttcagccat ccccggcggc gcgcgaaatc
    99721 gatacacccg ccgctgatcc ggtcgtagtc cgactcgatg gtgaggccgt tggagatgga
    99781 gcggaaaccc cgcacgcgga gggcggccca gtgccgtccg gcggtttcca gacaataggc
    99841 ctcgttcgga tcggcgatga gaaagctgtt gtggtagaac atccggcggt tctcgtaccc
    99901 gccgcaggcg tcctggccgt aacgctcgag cagatcggtc accgtttcca gggccgccgt
    99961 ggcggaggcg ctgcgctcca aggcgaaccg cagcaggtcc atgccggtca gggccggcgt
   100021 cttccggtcg atcccgaccc gcgtgaagac ggcttcgttg ccgatcacca ccccgcgctc
   100081 attgaccccc atctcgcccc cccacatgtg gaagggccgg gagaggagga cctcgaaggt
   100141 ccggggaacc tgcggaatac ggatataggt ggcctggaca aacggcgcgt ccgtctcggc
   100201 ggccggataa cgcacgatgg cctgcgcctc attcggctcc cggtccgaat tctttccgaa
   100261 aatcagatgc cccgacgccg tgaaatcggg cgttgccaca aaggtgtcgc acatgatcct
   100321 gacctccggt aagcagggac ggcctcatcc gatgaggacg acctggatgt ccatgacatt
   100381 cgttcccgtg ggacccgtga cgaaccgttc gccgagccgc tcgaaaaagg tgtagctgtc
   100441 gttgcgcgca agatattcgt ccgccgccca tcccctgcgc tccgcccgga tcacggtccg
   100501 gccgtccacc cgggcgccgg cggcgtccgt cggaccgtcc gtcccgtcgg ttcccgcgga
   100561 cagcatcgta atgccctcga cgccggcgat ttccatcgca aaggagaggg ccagctcctg
   100621 gttccgtccc ccgcgccccg cgccccggac cgtcaccgtc gtctcgccac cggcgagcag
   100681 gcagagcttg tcccggccgg atccggcttc gattctcgcc cgcagggcga ccgccgccag
   100741 cgctttcccg acctcgaccg cttctccggc cagctccgcc gagaggatcc ggaccgcgta
   100801 ccccagggat tcggcgcgac ggccggccgc ggccaaggcc agacggttgc tgccgacgat
   100861 ccggttttcc acccgggaaa aaaccggatc ccccggcttc ggggtttccg gcagttcccc
   100921 gcgccggccc cggttcaaca ccgcccggac ggcgtcggga acgtccggca agagccggtg
   100981 acgctccagg acggcccagg cctcgtcgta ggtggtcgga tcgggcgccg tcggcccgga
   101041 agcgatcacg tcaacggcgt cgccaatgac atcggacagg aggaacgaca gggttcgcgc
   101101 gggatggagg tgcgccgcca agcgtccccc cttgacccgg gagaggtgct tacggacggc
   101161 attcagttcc tgaatcgtag cgccggcctt caggagctgc tccgtcaccc gttgcttctc
   101221 cgtcagggtg acgccggggg cgggggcaac caggagggcc gatccgccac cggagaccag
   101281 attgacgacc agggtccgct cgtctaccgc acccagaccg aggatctcgg cggtcgccgc
   101341 cagaccccgt tcatccggca ggggatgccc ggcctcccgc acccggatcg ggtccaggga
   101401 gacctccgga acgcggtggc cgtccttggt gacgatgcac ccgtctgcga tcagatccgg
   101461 caagccccgc cgcacggccc gcgccatggc gaaggcggcc tttccgaatc cggtcacgat
   101521 caggcggcca tgcccctcgc gatgatagag ggcacggaca ccgtccagct ggtcgcggac
   101581 ggccggctcc ggatccacgg cggccagcgc cgcggcgaag atggcattga gatccgaagg
   101641 cggggcgctc atcggggacc gtcaggcggg cggtctggcg ctagcaacct gttcggcaag
   101701 ccagccggtc cattccgcga tcccctcacc ggtcttgcag gagacgggaa agatccggat
   101761 agcgggattc aacttgcaaa cccgctcgcg cagccgttcc agatcgaagt cggaggtccc
   101821 cagatagtct accttgttga cgaccagggc gtcacagacg gaaaacatga gcgggtattt
   101881 caggggtttg tcgtcccctt ccggaacgct caggatcatg gcgcgcctct gggaaccggt
   101941 gtcgaactcg gcggggcaaa ccaggttccc cacgttctcg atgatgatgc aatccagatc
   102001 cgcgaggtcg agttccttga gcgcccgatc gatcatcggc gcgtcgaggt ggcaaaaccc
   102061 tcccgttcgc acctgcaccg ccggcacccc ctcgcggctc accttctcgg catccacgga
   102121 ggaggcgatg tccccctcga tgacgccgat ccggacccgg tccctcaagg tcgacagggt
   102181 tcggaggatc aggctcgttt tcccggcgcc gggagaggac atcaggttca gcatgaagac
   102241 gccatgccgg cccaggtcgg cgcggatctt tccggcgagt tcctggttgt cggagaggat
   102301 ctcttcccga atgtcgatca agcggacgtc tgacatcata tcacctcgat atttttgatg
   102361 aaatattcac gaccggagac gagcgtcatc cgctccccgc cacactgagg acagcggacc
   102421 tgatctatct cgcggacatt gacgggaaag tcgtggcggc agggctcgca gcggaagacc
   102481 acggggacgc gctcgatgtg cagccgggcg ccggcggcca gggtatcctt gctcaggtag
   102541 tcgaagtagc gctgcagcca cgtgtcctcc aaatcgctga gctccccgat cctcagggcg
   102601 attcccagga cccggcggac gccgttctgt cccgcgtgcc tgagaaccac attcagaatg
   102661 ctctccgtca ccgccagctc gtgcatccgt ccctcccccg gtccggttcc cttccacgcc
   102721 tcggccatcc ctgggaccgg gatcgaattt tataccaccc ctcccccgga aaaacaatcc
   102781 cttccggcga caacgccgtc tttccaattg acacgtgcgt gacgcttctc tatacttcgg
   102841 ccagcttagc gcgtgtaagg tttagcgggc aggggcgcga ggcggcccgt cggtatgaac
   102901 gaggcccttc ccgaccgtgg agcggctcct gccacgagga gaaacgatga aacggccctt
   102961 gatcaccctc ctgtccgtcg gtctggttct ttggatggct gccatggccc gggcggaggt
   103021 gtcgtccgtc accaaaatcg gcaatgacgt gatcgtcagt gaaggaacga cggtcaagaa
   103081 tgccacggcc atcggggggc aggtgaccgt ggacggcacg attcagggag atgccgttgc
   103141 catcgggggc tccgtcgttc tgggaagcag cgccctagtg gcgggggatt gcatctccat
   103201 cggcggggtc atcgtcaagg ggcggggagc gg
//
LOCUS       DAOOLJ010000002        99562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_79_length_99562_cov_17.678354, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000002 DAOOLJ010000000
VERSION     DAOOLJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 99562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 99562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99562
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_99562_cov_17.678354"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLW16_00465"
     CDS             <1..617
                     /locus_tag="PLW16_00465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB69405.1"
                     /translation="IVGLDASPERIAHAQRCYGSRAGIEFAVHDLRESLSRFGTFDGA
                     WVRFVLEYFQRESLEIVRNIAASLRSGGWLYLIDLDHNALNHYELPAPMAKILPEISR
                     CLEEIYDFDAYAGRKLYAHLFDLGFEDIEVSVTAHHVIYGQSREVDSYDWTKKIEVTA
                     PRLTDLFSMYPGGYDRFMADFREFFHNPRRFTYTPLILCKGRKP"
     gene            complement(629..2950)
                     /locus_tag="PLW16_00470"
     CDS             complement(629..2950)
                     /locus_tag="PLW16_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB69406.1"
                     /translation="MNDQALYNSRNIKVYMEYLGTHYPDLDAQTILDYAGMTIYQLND
                     GGHWFNQTQIDRFYEMVVRMTRNPNIGLEVGRFAPFSKAAGAMTHYVAGFINPKTAYS
                     VVAKLYNQVSRACLLQARALKSNQAEITVRLKPGVEEKLYQCENRWGALEGISKLLTG
                     RFPHIDHPVCMHKEGDCCRYIITWEPTRSSLWKRIGTYTALVSLVIVLALFIGFANRY
                     AFAVSSVALLLILGAFMRASVLEKEELKDLLESRGDAAELLLDQVNTRYSNAVLIKEI
                     GQYVSSALDTELLLKYLLSALEKNLEFSRGMVMLANGDRTRLEFTAGYGYSPEDEDFL
                     KKTEFSLANPHSRGAFVVSFHKQTPFLVNDLREIEQDLSPRSLDFTRKMGSRSFICVP
                     ITYEGRSEGILAVDNLRSDRPLNETDISLLMGIAPQIGISINNARIYEKLQEREKRFR
                     ILAESAPDIIFTLDNDGKLLYVNPAWKRILGHPADAVVSLPLTAFVDEQDSHRLVQLL
                     KGVRENRETLMDALLTIPDCSGIEHNFSFNCAPNIDGEGRVDSLVGIFKDMTDLRRSE
                     VELKKSYQKLQLAMSSTISVISLIAESRDPYTAGHQRRVADLAAAIAREMELSEERVT
                     TIHMAGLIHDIGKINVPAEILSKPGKLNSGEFALIKNHPETGYNILDKVDFTPPIAQI
                     VYQHHETMDGSGYPRGIVGRDIILEARIITVADVVEAMASHRPYRPALGIEKALDEIR
                     THRGVTYDPDVADACIALFTSGRFHFKDPGIKD"
     gene            complement(2947..4998)
                     /locus_tag="PLW16_00475"
     CDS             complement(2947..4998)
                     /locus_tag="PLW16_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69407.1"
                     /translation="MPIRLFLHRDLLEATRSAPVIGKKHLINTIHRLHFMNGNILVHV
                     GDPEHQEDLLVQGRLESCAAGEIRCLWAQGAPRFSENAQPLHVIVEDGLSLILFPVRL
                     TGCDETGFSAVLPETGYLLGKRRARRHACRDTEVTLTQSGFSAQGDLVDFSPFAFRVR
                     LEAEPGLSFIWMNPDDSITICLRKGERILFSGPCRCLRQTGNLRKREMVLAPLRGEIQ
                     RFRKQKTRTPRLRLTPPPSVHFVHPCLGKPMRRIVTDLTFSGFAVEEQADDTVLMPGL
                     ILHDLEIRPAGASGITCTAQVIYRRKIAKGRIRCGFAILDMDFRAYRHLSHLMVHSGS
                     SLARFTCEIETDALWEFFFDTGFIYPQKYHLIQSAREAFKDTYRRLYREDQDIEAHFT
                     LQENDRIYGHVSIFRAYQRAWMVHHLAARPLNGKRTGLFVLKNILKFFDGLYRYPSIR
                     IDHMMFYFRPENYFTNLFFGGFARDLGNPRACSLDLFAYLSHPMDGPRSSLPPDWQFG
                     AFETRHLPEMEQFYRNASGGLLLDVLRLGQTDPSCEPLEEVYRRHGFLRQCHIYELLH
                     EQALKALLVVDRSSPGFNLSELLNGIKVVVTDPAGLPWRVLSAALDRLAVDYGTDSVP
                     LMIYPATYPSQEGISVEKRYLLWILDTQYGREYVEYMENKTRLTLRFLLKHLLGKMVS
                     K"
     gene            complement(5244..6692)
                     /gene="gltA"
                     /locus_tag="PLW16_00480"
     CDS             complement(5244..6692)
                     /gene="gltA"
                     /locus_tag="PLW16_00480"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPB69408.1"
                     /translation="MQRTGADSMATNMTTDRKDKKEKIPRQPMPEQPPAERVRNFAEV
                     PLGYTEAEAVLEARRCIQCKKPGCIAGCPVEVQIPAFIRLIAEGKFVEAARKLKEDNS
                     LPAVCGRVCPQEDQCEKSCILGKKGEPVAIGRLERFAADHERNKGEVFIPAPAAPNGL
                     RVAVVGSGPSGLTIAGDLIKLGYGVTVFEALHKAGGVLVYGIPEFRLPKAIVEAEVAY
                     LEKLGVRIERNAVIGRLKTVDDLFAEGYGAVYLSVGAGAPVFMNIPGENLSGIYSANE
                     YLTRSNLMKAYRFPEYDTPIVRGKKVAVIGGGNVAMDSVRTALRLGAETATIVYRRTE
                     AEMPARKEEVHHAKEEGVRFELLTAPLEYLGDDQGWVTGTRCRRMELGEPDASGRRSP
                     VPVPGSEFVLAADTVVVAVGTTANPIVPATTPGLETNRRGYIVVKGEDGATSRPGVWA
                     GGDIVTGSATVILAMGAGKQAARAIHAFLSKA"
     gene            complement(6658..7494)
                     /locus_tag="PLW16_00485"
     CDS             complement(6658..7494)
                     /locus_tag="PLW16_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPB69409.1"
                     /translation="MNKITLRRQLSENVVRMDILAPEIARRRRAGQFLVLKVHEQGER
                     IPLTMVDSDPAAGTVTLIYQVVGKTTALLAGLGVGDTILDVQGPLGNPTEIETFGHVV
                     CVGGGVGVGVIYPIARALKEAGNHVTSIIGSRTKDLLILEEETGRVSDRLIVATDDGS
                     YGFHGFVSQVLQGLIDRKDPIDRVFAIGPVPMMRVVCDVTRPHAIPTIVSLNPIMVDA
                     TGMCGACRVSVGGETRFACVDGPEFDGHQVDFKLLASRLRMYCPQEEEAYAAHRCGQH
                     GN"
     gene            7792..7944
                     /locus_tag="PLW16_00490"
     CDS             7792..7944
                     /locus_tag="PLW16_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69410.1"
                     /translation="MTSTSPSSPGKIPVRIDPDLADLISGFLDKRRQDLADYLDRIEI
                     AYEQPD"
     gene            8102..8908
                     /gene="truA"
                     /locus_tag="PLW16_00495"
     CDS             8102..8908
                     /gene="truA"
                     /locus_tag="PLW16_00495"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPB69411.1"
                     /translation="MTRYKLTLEYDGTGYRGWQTQQNARSVQGTVREAARTLLGAAVD
                     LQGAGRTDAGVHALGQVAHLEAAVRMPVRKLQEGLNDLLPAGIHVLRTEETPPDFHAR
                     HHARARSYVYLISRRRTAFGKRYVWWVRDRLDVKAMQSAAADIAGFHDFASFADRRLE
                     KGHSTRVQVERAELFERGELIVFRIAGSHFLWKMVRRLVGTLVEIGRGSPPPREMKTL
                     LNRVSEAPAAWTAPPSGLYLEQVLYEGDVWNPEPFAPLPLWGLSGARDRS"
     gene            9173..10369
                     /locus_tag="PLW16_00500"
     CDS             9173..10369
                     /locus_tag="PLW16_00500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB69412.1"
                     /translation="MKYPDIPFEPFFSWVCRPRVMYAPGLRSEVGYEMAQLGGKRVAI
                     FTDKGLVDAGVADQIVEAVRNSDLELAGVFDGIVQDARIDIINAGAKFYRGKGADCMI
                     ALGGGSVMDTAKAVNIMIGGGEEDFGPLAAQVALYEGAKPLPPHIAFPTTAGTGSEVT
                     IAMVVLDVEAKAKISVAHPYCNCDLAMLDPELMVKLPAKITAFTGMDALTHAVEGATS
                     TSAEPISDALCLHAIRLIFKYLPIAVKEPDNVDARGYMLLASCIAGMGFSNSMTGAVH
                     ALAHALGGQCGIPHGLANGILLPHVMDFNVSERPERFMMVADAMGIPVDGMEPEAAGR
                     LAVQAVKDLLKEVGLTQTLKDLGVPTDRESLKPLMELASGDSQIPYNPRYCEEDDILD
                     LYMKAL"
     gene            10393..10812
                     /locus_tag="PLW16_00505"
     CDS             10393..10812
                     /locus_tag="PLW16_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPB69413.1"
                     /translation="MEFWKDESEPIKAFLALFDASAKDPELSEGLKKVNQLIWFDYTE
                     SGPNCSFWVDTRGGALKNGAGKPDGEPDLIMSLSADDAHRSWSDKLNPVMAITRKKIR
                     VKGSATGLLKLAPKLKKVAAIYMTVLKDLGWEDKIIK"
     gene            11032..12897
                     /locus_tag="PLW16_00510"
     CDS             11032..12897
                     /locus_tag="PLW16_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPB69414.1"
                     /translation="MNGNTGKIVSVDLTAGQATVETLPGEDYRKYIGGSGLAAKLFWE
                     RGNFAADPLSPEAMLLFMNGPFAGLRLSGASRNSVAGCSPLTGHWGDSSCGGYFAPEL
                     RYAGFDGLILTGKAARPSLLLIVDDKVTIEDAGAYWGKGTEEVTQALKAKYGKDYRTL
                     VVGPAAECGVRYTMILNDGHHATGRAGFGAVMGAKNLKAIVVKATKKEMTLADPEAFE
                     ALRKDLNVRIKQALASEVLHENGTAANLMGGVYSGDVPVKNWTSNFWEEAADALTGST
                     LTDTYLTRRGACAYCGIACKRYVEVKDGPFAVPDGPGPEYETIVAFGTLIGSIDLAAA
                     CKAGRVCNDLGMDSISAGGTIAWAMEAFERGDLTLEDTGGIALNWGDMDTVVNRILPM
                     IARREGKLGDLLADGSVAAAKKIGRGLDYTVHSKGLEAPMHDPRGGGHGMALTYAVSP
                     RGACHVADPMLFVEMGARYYPEIGFDFILEPKTSENKAEAAVTSVALGAIENSACFCQ
                     FADAEVTIPDWLALFNTVAGYNWEAEDMLAAGRRVFFLKRLINYRYGLTAADDALTPR
                     MLEPGKDGEAEGSQVDLEAMKSRFYELMDIDPVRGIPTPAALAALDMSAEAQAVA"
     gene            complement(13031..13570)
                     /locus_tag="PLW16_00515"
     CDS             complement(13031..13570)
                     /locus_tag="PLW16_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPB69415.1"
                     /translation="MKTDKQTDRQTALRIFFLMALVLVFAATSGGSLLAAPKKLATAK
                     AGDCAACHGADKVLPDGHAPTAAMNGEACLGCHQKGSPLALYTKMPLSHFHQLKGITC
                     QACHTDLNAPQELSTEQCLACHGSFETVAARTANVKPQNPHSSPHGNTYTACDLCHHQ
                     HAKSENFCAQCHNFKFVVP"
     gene            complement(13655..15202)
                     /locus_tag="PLW16_00520"
     CDS             complement(13655..15202)
                     /locus_tag="PLW16_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPB69416.1"
                     /translation="MKEKEKAVSRRDFLKVTGGTAAGIAVTGMAWRPGEAEAAAMPKK
                     WNETFDVVVVGSGFAGLAAAYEAKKAGASVVVLEKMRTPGGNSIINGGVVSAAGSPLE
                     ARDGIKDSPELLLKDMLAAGLNLNHLELARLVAYESNNTVQWTINELGVKYKDSLSQE
                     GGHSVPRMYSTYNQSGSAIVTQQLEKLKEIGLIPRTQVLLKRLLRDTDGRVKGVEVRE
                     KYVFPRENSGRTKFIKAKKAVVLATGGFGNDVPFRTIQDPRLTADLESTNQPGATGEA
                     LREAFRIGCTPVQLSWIQLGPWGSPDEKGMGLNPFFAQLCCAMYGLWVDTQTGKRFVN
                     ELADRKIRADAIIRVGNKCIAFTDANGFAIGQKMLGDIMAKLMERGVVKRYDSLAAMA
                     EAYDCPIAPLRETIANFNKAVAAGKDEEWGRYLQKNQRALDTAPYYASRLMPKVHHTM
                     GGVNINVEAQALNVEDDKPIPGLYCAGEVTGGVHGAVRLGSCAVLDCLTFGRIAGRNA
                     VREKAWG"
     gene            complement(15439..15678)
                     /locus_tag="PLW16_00525"
     CDS             complement(15439..15678)
                     /locus_tag="PLW16_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPB69417.1"
                     /translation="MTATTQKTGTNLNSLHGIMTISAWGFVIVIASFLFLYLGYRLDQ
                     WLGTAPNFMLGLFFLAIVTTIGRLYREAWVRKEQK"
     gene            complement(15729..16004)
                     /locus_tag="PLW16_00530"
     CDS             complement(15729..16004)
                     /locus_tag="PLW16_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69418.1"
                     /translation="MKKTVSTLIVTIAVVLLTATFSFAEYAVGAGDAFPYFQLGCLIV
                     GGLIIVSLKHKYEKIYLGEAVGAFALYTVLVALFTSPVVNVVKTLVS"
     gene            complement(16274..17131)
                     /locus_tag="PLW16_00535"
     CDS             complement(16274..17131)
                     /locus_tag="PLW16_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HPB69419.1"
                     /translation="MAGHSLPVDRIVRYVLERRCREGGFCFYRLEEPNGSDTYYALSI
                     LHLLGVPFREDRTLGFLKARQQQDGAYESIFQAFYCLQSLKLLGEEPEADPTSYILDH
                     LHVYEIEKLPAEVTSIFRRMLMLVELCTARHIALAPSLRETVIRFVMHFRKEDGGFGA
                     FYATLLETAQALRILDRLGYPLQILGSEAFVSQCADPVCGFVDIPFTTFSFLEHIHAG
                     VVASAYLNRPPPHADACLQFLSACQTLSGGFSRSTQAGIATLEDTCLAVETIDLLRKF
                     ELIGWRLCD"
     gene            complement(17118..17762)
                     /locus_tag="PLW16_00540"
     CDS             complement(17118..17762)
                     /locus_tag="PLW16_00540"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB69420.1"
                     /translation="MFVGREKDTARIIRALERGQNVVLTGIHGIGKTILIKHIAALTE
                     KNWRFVFVDFSLAPTQVCRRLLKEFISAGRSTGEIEARTYKSLRYLTANIALKDSRQH
                     IIVLDNLARLTAAKASLLRYLILEKRYLFVAIAETFLPARDLFRLRAELLPAIDVVVK
                     RLPIEETIAFFNDARRRYKLPWTDADVRMLSEASGCYPLGMKDTVSRERNRGWT"
     gene            complement(17826..19115)
                     /gene="eno"
                     /locus_tag="PLW16_00545"
     CDS             complement(17826..19115)
                     /gene="eno"
                     /locus_tag="PLW16_00545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB69421.1"
                     /translation="MSPKIKRIHAMEILDSRGNPTLRAFVTLDDGISASASCPSGAST
                     GEHEAVELRDGDKKRYGGKGTLQAVHNVNKTIAPALIGMNPLAQEQIDRLMIDLDGQP
                     NKGRLGANVMLSVSMAVARAASLSLSTPLYTYLAGSRKIRLPVPMMNILNGGKHANNS
                     IDFQEFMVMPLGAPTFAEALRYGVETFHALKGILSKKGHVTGVGDEGGFAPNLKSNEE
                     ACEVILEAIEKAGYQPGRDVALALDPAASSFYEGGRYRLVKSGQGEKDAAAMNRLYDS
                     WLQKYPLVSLEDGLAENDWEGFQAQTRLLGTRIQIVGDDIFVTNSRFIKRGIEEKTAN
                     AVLIKLNQIGTVTETIEAVDLCRKAGWGIVISHRSGETEDTFLADFAVAMGGGQIKTG
                     SACRSERIAKYNRLLEIEAELGADACFENPMKKGSTK"
     gene            complement(19156..20808)
                     /locus_tag="PLW16_00550"
     CDS             complement(19156..20808)
                     /locus_tag="PLW16_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPB69422.1"
                     /translation="MPDLLSDACGQLMARMRDAVERFQMISLRRPLDAAEALLRQNSP
                     IAVAVLGQFKAGKSSFLNSLIGRTLLPVGVVPVTTVITRMQYGPKDLARVRFFDGANR
                     EIGLDSLADFTAEAKNPSNEKNIAYVDIELSGLQDYEGLCLVDTPGLGSVFQAHIRES
                     ENWLPEVGAALLAISADRPLSEQDLHLIRDLTAHTPRIVLLLTKSDLLDPDQQEEVVT
                     FFQDTLQRELNRKFPVLLYSTRQETERFKTRLEMEVLLPLAANRDREFRNILKHKARS
                     LARGCLGYLEIARKMSQQADQDRKGLRQRILDERVNYTLLQEELTVITRENSRQTRTF
                     IMGNLNPLAAALREKLATQLKRDMATWTGNLWKLTRRYEDWLTENFTEEIGRISRTEQ
                     AHFMGTLKKAHASLDRSLASFRALLSDNIERVLGVRLAEADWRIEVAEPEHPDIHMAK
                     TFDFHFDLLWFLIPMTLFRGLFERHFLKMIDREVEVNLSRLAFQWETRINAAMEEMRK
                     QAERYIRDELTTIEALLSRQQGQTLDIEKNIQEIVNLFEKVK"
     regulatory      complement(20890..20954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            21157..22542
                     /locus_tag="PLW16_00555"
     CDS             21157..22542
                     /locus_tag="PLW16_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPB69423.1"
                     /translation="MTDPKSPLRSFFSSVRLALFLLIAIALTAIAGTVIPQGSAAPEF
                     AARLTPSMAALLQKLQLFDVFHSLWFLILILLLTVNLVVCSWSRFPASLVRWRQARSP
                     APAPEGAFDRLSSGNRIRVTGEVPAVAAMVETRLRKRVGPVRTAPGRDATILSAQKGA
                     ASHFGVYLVHLGVLVVMAGVVLGFFLGFEGSLQLGEGEAADAVRTKAGGVQRLDFTVR
                     CDRFVLEHYTGGMPKTYRSDLTFLKGGKVLRRTPVLVNHPVTFGGYRFYQASYGTIPG
                     GGATLALRREGRALGSVEVGVGAGFNLPGGEGRVEVQRIEENLMHMGPAVKLLIRTPG
                     EERPLWVFQHLETILKEQPNLFQMMPMLNPAGFTPYEFALARLSPRYYTGLQVARDPG
                     APVVAAGAVLLVVGFLFVFFYAHRQVWVRVAAENGAVEIAVTGRTNRDRVGLEREIAS
                     LLKGFREGGAG"
     gene            22539..23366
                     /gene="ccsB"
                     /locus_tag="PLW16_00560"
     CDS             22539..23366
                     /gene="ccsB"
                     /locus_tag="PLW16_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPB69424.1"
                     /translation="MINTEILSWVTFIYLGAFLLYLLRMVNGREFWGVGASGAVVLGW
                     IAHTAGLLIRWRESHALGIGHAPMSNFYESLIFFAWATALFYLIVEWRTRNRSLGTFV
                     LPIVFLLMAFASLSPGMNDRIQPLIPALQSNWLTVHVFTCFLGYAAFTAAFGLGIMFF
                     LKRLDRPARFVGLIPAAETLDELLYQSVVLGFVLLALGIMTGSVWAHYAWGSYWSWDP
                     KETWSLITWLIYAALLHARYVRGWRGNRIALLSVVGFAAMLFTYLGVNYLPSLHSYL"
     gene            23540..24124
                     /locus_tag="PLW16_00565"
     CDS             23540..24124
                     /locus_tag="PLW16_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HPB69425.1"
                     /translation="MKKSRLLLILVLVFCCLPFPSALQAVDLQLNRTPVKVFFSPSGG
                     CTDAVVTEIDQARSEVLVQAYVFTSAPIAKALLQAHRRGVRVEAILDKSQQTRRYSSA
                     TFLVNAGSPTYIDGQHAIAHNKVMIIDGERVISGSFNFTKAAEESNAENLVIIRSAEL
                     ARVHLANWREHRAHSTAIDRLTLVQPSAGRPQEP"
     gene            complement(24368..25228)
                     /locus_tag="PLW16_00570"
     CDS             complement(24368..25228)
                     /locus_tag="PLW16_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPB69426.1"
                     /translation="MSRKVIVTCALVGSSTRKSQNPNVPYSPKELAEAAALAHKAGAA
                     MVHVHGIEDDGTNTTRVERIREGHDAIKQKCPDLIVNLTSALGPGATPEQRIGQIVAV
                     KPEMASLNMGTMNFAAVNRKTGEVVVDYTFENSLIVISDFVRQMKENGVKPEMECYTS
                     SHIDNFYAISGSGHFTAPLNFNFVWGVCGGQSFRPAMFTSMVQALPAGANFTACGVGN
                     EEWPVITQSILCGGHVRVGLEDNIRMPNGELAKGNYELVEAAVRIIDGLGLEPATPDE
                     ARQIMGLTRR"
     gene            complement(25318..26853)
                     /locus_tag="PLW16_00575"
     CDS             complement(25318..26853)
                     /locus_tag="PLW16_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69427.1"
                     /translation="MENNISRFLDDNVTIFGEHDQFIYLGPEGEKRFTNTEIRRRARA
                     LSTGLRKIGVKKGDIVGAVVGNIPEVMEIINGINRGGGVYLPIIFMLTALEIRYILED
                     SKCKVVITEDSLLAKVREATQGVATIEKIIVIGKETGADITPYASLLTEIDNRGDVVP
                     VDPDDMAILMYTSGTTGFPKGVMLSHRNMESQMRTGQSVWGGKKGQIMLTTVPMNHIY
                     GVISCLELYSLGCVSLMMPPFDPRKVLDAIRDYKVSFIPVVPTMLIYLMMVYDPAKDD
                     LSSLDLLICSGGPLALETLEKAQKMFGKEITQGYGCTEVGGSIARQRRDWPRKPGSVG
                     FPLPGLALKLVDLNGKEVPRGEEGEVICKGPIIMKGYLNKPKETSLALIDGWLHTGDL
                     GKMDDDGELYITGRVKDLIIKGGENIDPGVAEGWLYKHPAVWECAVIAIPDEKYGEEV
                     AAVVTLKPGQKATEEELLAYLNQHLHHFVAPKKIFIFDALPKTGLGKILKREIRKIVN
                     EKL"
     gene            complement(27062..27220)
                     /locus_tag="PLW16_00580"
     CDS             complement(27062..27220)
                     /locus_tag="PLW16_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69428.1"
                     /translation="MGKIEELYQTYVERIMDWYDGLEQLYQYGVLFLVIVVGLLIFAF
                     FLLRRITK"
     gene            complement(27282..28883)
                     /locus_tag="PLW16_00585"
     CDS             complement(27282..28883)
                     /locus_tag="PLW16_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB69429.1"
                     /translation="MQTHTEIWVVADLRSERLFGYTLNLLAKARELAQAVSGRAAVVV
                     LESPSPAPAEPGGLTPDEADARGLAHGADLVYRIADPLLAAPRADCFALALASAVRQR
                     APRLVLCALTDFGRELAARTARLHDAGMIADCADLRWQAGGIVASCPSWGGEITAEIT
                     YADPDRTGFATVQPHIRAAVAFAGTPGVAERLPIDSLTPPPGLTLLSRETEPEGRRCL
                     EDAETVVVGGGGLGSADGFHLLRELAVALGAEVGATRPPVAQHWVDEDRLIGQTGKTV
                     RPRLLVSVGTSGAIQYTAGIAESETIVAVNRDAEAPIFALADIGVVADARTFLPLLTA
                     KVKQTVMRGLADLIWDENEKDGAESFGDRIRKIREARDWTPAALAEATGQTPEFIARV
                     ERDEVAPPVAFLLRLAAALGVDPSTFLSREEKTHLRDQRAQAYIKRTQNYSYQNLTPE
                     AAHDHLKAFLITIDPKQAHKPVAYKHEGEEFIFVMAGELELTLGSKAHHLHPGESIHF
                     NSDTPHKLKSLSNVETRCLVVLYTI"
     gene            complement(28894..29664)
                     /locus_tag="PLW16_00590"
     CDS             complement(28894..29664)
                     /locus_tag="PLW16_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB69430.1"
                     /translation="MNIICCLKSVLLAAPEGQVARTADLSDLNPFDRPVLETALRLRE
                     ETGGRITALSMGPPAAADALRQALALGADRAVLLCDRALAGADTLATSTALAAAVARL
                     APYDLLLFGTRTSDSDTGQVGPQTAVRLGLPLVTWVHALERTASGFVAERRADGFRER
                     FAVTLPAAMTIHPAAVTPRDETLGGIDAAYEHGVIETWGLDTLGCDPAEVGEAGSPTR
                     VVSLAKKTRDRKCELLTGASEEVAEALVRTLADRGLIG"
     gene            complement(29668..30810)
                     /locus_tag="PLW16_00595"
     CDS             complement(29668..30810)
                     /locus_tag="PLW16_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69431.1"
                     /translation="MNTFLTDKNRAVRRAVRAFCEREIAPIAKQIDQEASFPWEVADK
                     MGRLGYFGIQAPKTLGGAELDTVSYVLIIEEISRASGGLGLCVTVHNSVAVAPLLAFG
                     TEAQKQRWIPPLARGEKIGAFCLTEPNAGSDASGIEATALRDGDHYCVNAGKVLVTNG
                     GIADLCLIFARTDPKAGRRGISVIVAERGTPGFSVGDLEDLCGMRANPVSSIRLADCL
                     IPVENRLGREGMGLAIGLAALDTGRIGIAAQAVGIAQAALEESVRYAKERRQFGVPIA
                     RHQSIQQMIADMATDLDAARLMVLRAAVRRDRGDPFSQASAMAKLHASEAAMRITDQA
                     VQIHGGYGYSKAYPVERYWRDARVTRIYEGTSEIHRMVIARGVLGD"
     gene            complement(30928..31809)
                     /locus_tag="PLW16_00600"
     CDS             complement(30928..31809)
                     /locus_tag="PLW16_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HPB69432.1"
                     /translation="MNGPWIWIDSPDKLRDARQKIFQSRALCIDTEYDSFRYFRERLC
                     LIQIRDGATTYLFDPLDSLDFSFLGGPFADPAVLKIFHAGDNDIRLLKRDYGFQFRHL
                     FDTQRAASLLGSQHLALSTVIDTFLKIDLDKTKKMQRSRWDRRPLSDAQIRYAIRDTL
                     FLFPLYETLSALLAERGLIEQGEAVFAGMAAVVWQEKTPHPRAHERIPGYEVLTDNER
                     LRLRRLVEWRFAKAREVDRSAFMVLSDQELLRLSQSRTPSLTFFREQGLLPPGKIDRY
                     GTEVLAVLAAAGTSPAG"
     gene            complement(31951..32748)
                     /locus_tag="PLW16_00605"
     CDS             complement(31951..32748)
                     /locus_tag="PLW16_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPB69433.1"
                     /translation="MIPAGQYLPGDGPIHRLDPRVKILWAIAFSILLFQAGLAGGILL
                     TALLAAVGAMSGIPFRRFLSALGPLWVLVALLFLLHAFFSNGPVLFALPGLPIALTST
                     GLARGFTVAWQFLCLVSAGLFLTLTTPPGLLVLGLERLLKPTGRLGVPYTELAFTVSI
                     ALRFLPMLHETFLQVRDAQRSRGSDTRSGTLPGRLRAVSALVAPLLLGTFRQADALVE
                     AMEARGYRPGPRTGLTTPRFTPPDVAACVLLGLIVALMTLPLALRLF"
     gene            complement(32745..33605)
                     /locus_tag="PLW16_00610"
     CDS             complement(32745..33605)
                     /locus_tag="PLW16_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB69434.1"
                     /translation="MIQVQTVTFTYDGESRSVLRGATFVVAPGEHVVLSGPNGCGKTT
                     LLKHLNALLVPTAGEVLIDGLSTRDRRHAPEIRRRVGMVFQNPDRQIVGMTVEEDVAF
                     GPGNLGLPPTEIRRRVDAALAAVDLDALRHRSPQALSGGEKRLLAIAGVLAMEPRYLA
                     LDEPTAYLDPVGRRRVLDVLDCLQREGIAILHVTHDLDALLNADRLLSLDGGVIRHDG
                     PPRVILKEPGAWSGIGLETPPVARLLERLRQDGVDLPADVLTVEEAARALQGLLAGRD
                     AGGRTEGGPP"
     gene            complement(33616..34158)
                     /locus_tag="PLW16_00615"
     CDS             complement(33616..34158)
                     /locus_tag="PLW16_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPB69435.1"
                     /translation="MKDAAGPITLRGMTRAALFGALTAVGAFILIPIPPVPVTLQTLF
                     LYLAASTLGGALGAWSQTVYLLLGIIGLPVFAGGKAGLGVLIGPTGGYLLGFVPAAWI
                     AGHLIALKARPGFAWVAFSLAAGTACLYGTGVAQLAVVGKLGLAKALTVGILPFLPGD
                     VLKIAAATGIFLKVRDRLKR"
     gene            34468..35772
                     /locus_tag="PLW16_00620"
     CDS             34468..35772
                     /locus_tag="PLW16_00620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005334.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB69436.1"
                     /translation="MRFDIAKSGSGLTYEIRNIVAVANKLKSYGREVVWENIGDPINK
                     GEKIPAWMKEVLVKILEEDRSYGYSPTKGMNETREYLADKVNRRGKVQISPEDIIFFN
                     GLGDAIARAYSSIRIDARVILPEPTYSTHLLAEVLHASFPPNTYRMNPYSGWCPDIRE
                     LELKVKSHHAIVGILVINPDNPTGFVYPEENLRQIVQIAKEHDLFLVFDETYRNIVYN
                     GKQTVPLSDIIEDVPGISMKGISKEFPWPGSRCGWIEVYNADKDETFSRYIGAILSQK
                     MSEVCSTTLPQIAIPRIMEHAEYDRYLNERVRHYEKLSNIAYDILSDVPCLIVNRTNG
                     AFYMTAVFNEAILNNRQALPIENDEIRQFIEKLVHDKIEPDKRFVYYLLGATGICVVP
                     LTSFFTALPGFRMTLLDKDEKEFEQVVRTIAESVTRYINSAT"
     gene            complement(35883..36164)
                     /locus_tag="PLW16_00625"
     CDS             complement(35883..36164)
                     /locus_tag="PLW16_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69437.1"
                     /translation="MFKSKLNPEVSAETLKGRVWIGVFLLAIWLVFLWWAGITFPFVG
                     HDANGQNIVYGVIVGIPFLVLITLYMALAVKDLKEAVAAMKAFSKGNRK"
     gene            complement(36151..37086)
                     /locus_tag="PLW16_00630"
     CDS             complement(36151..37086)
                     /locus_tag="PLW16_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HPB69438.1"
                     /translation="MYFGPHLRIESWMFEIVLYLFFILVFWAGTKIIGREKTSLFLWG
                     SLIWTLTVENAMVIIGAYDYFAYANYYCWADKLIPGFSGWSCMVFFVPLSISIGWFIF
                     GLSAFIISDRLLPQGNIWIKAVVASVMMVSLDMLMDPISVVNEWWRWTVAGFYFRGVS
                     VGNYIGWFFMLFFFAGIYERSVIEKGSFAWLRPIEGLIFRRNTKDLSDTDSQTLKKIF
                     YFRTVAFIPVMLFVTILASQPLKQIGFNRYAPFNSVFPTTYDQKYPASAKPADMAPAV
                     IPDGDPRKVEGPRCEIAKGANVPTQPAGGASHVQK"
     gene            complement(37265..38728)
                     /locus_tag="PLW16_00635"
     CDS             complement(37265..38728)
                     /locus_tag="PLW16_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69439.1"
                     /translation="MPNKPFTIPKNKEEIRAWIKSHPAWEATMATDALFFPAEKLRTM
                     QNENLVKQMDRLEKFSPHYRAKFKEWGLDPKSIRTVDDLVKVPLTSKEEFMKDRGMSF
                     RLDMDPANMLDYIMYEVLYTTGTTTGMPSPFFNTTYDMFMQCWMFQIGCKICYMEPGD
                     MVMNLFPYHFIPHMGYYKTVHFAAAANLRMCWGFTGAPLPGFPYEFHRSLQQSIEDIE
                     RYKVNVLSGIGSYVRRLIMVAEEQGRDFSSVKSVQALGEAVPMGMRDDIVRRLQRLGA
                     GEVFVSNAFGFTEAQSAFQECRPLGGAHGGGPLCYFEVVDEQGNRKPDGESGLLCITH
                     LDRRGTCLLRYLVGDIVAITDEPCEHCGRNDQRIIAAVGSTYGTRTKELMKVKGTLIN
                     PEIMRDAVANTAGVIEYQFVVTKENADDPYSMDLLQLRIGADKGVDLAELERTLKEKV
                     KRAVELTPKIIFEERSAIFNPGETLKATRVVDMRPKE"
     gene            complement(38944..39378)
                     /locus_tag="PLW16_00640"
     CDS             complement(38944..39378)
                     /locus_tag="PLW16_00640"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPB69440.1"
                     /translation="MKRIALETVIMAALVFAAAPLWAASPIGKWKTIDEKDGKAKSIV
                     EVYEQNGKIYGKFLEMLNPADKNKVCDKCEGADKGKPIVGMVFIKGLKADGDEYTGGT
                     ILDPGSGKIYKGKLKVVEGGKKLKASGCISFICKGQTWLRVE"
     gene            complement(39606..41147)
                     /locus_tag="PLW16_00645"
     CDS             complement(39606..41147)
                     /locus_tag="PLW16_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB69441.1"
                     /translation="MENSNISSILDLNIETYGEYDYLIYLGYEGEKILTNREILVRAR
                     RFSVGLQKIGVKRGDIVGSVLSNVLEIIEIINGINRMGATYLPIIYMLTAAEIRYILK
                     DSGCKVILTEANLLPKVLEAAKGLPSVERIVLIGEGEGDNIVPYESLLTEDDGRGDAV
                     DVDPDREIAVLMYTSGTTGFPKGVLLSHRTFRFQFTSGQMVWGGKVGGRVLTTVLMNH
                     IYGVLSITEGNKGGGVCVVMQPFDPRRVLDVIRDYKANYVSVVPTMLIYLLMVHKPEI
                     DDLSSLVLLICSGGPLAMETLNQAQRAFKMEITQGYGCTEVGGSITRQRLDRPRKPGS
                     VGAALPGIALKIVDFQGKALPPGEEGEIVCRGDCLMNGYLNKPQETASAIIDGWLHTG
                     DLGRLDEDGDLFITGRLKDLIIKGGENIDPGVAEGWLYKHPAIWECAVIAIPDPKYHE
                     EVAAAVTLKPGMKATEEELLAYLGEHLHPFVAPKKIFIFDALPKTGLGKILKREIRRI
                     VHEQI"
     gene            complement(41224..41448)
                     /locus_tag="PLW16_00650"
     CDS             complement(41224..41448)
                     /locus_tag="PLW16_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69442.1"
                     /translation="MSLSSAYDLTTLVKPPRTFFINYPLGHTSGKPFDRANQTAILKD
                     ALGGAREINTPGAIVALPYAWDDPFWLAGA"
     gene            complement(41512..41988)
                     /locus_tag="PLW16_00655"
     CDS             complement(41512..41988)
                     /locus_tag="PLW16_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HPB69443.1"
                     /translation="MKYLKYVDVVQSMFPDQPPYQWTVNDSVPWTPIVKPLAESAVAM
                     ISSSGMYRKDQAPFKPDKNDLSFREIPADTDVRDLAISHDYYDHRDAEQDVNCVFPIE
                     RLRELAAEGFIGGLTPFHLTFMGRVFRKTQFLGEMIPAMTARIEAMKADLVLLVPA"
     gene            42263..43147
                     /locus_tag="PLW16_00660"
     CDS             42263..43147
                     /locus_tag="PLW16_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPB69444.1"
                     /translation="MTKESKHTLAALWPTIRQAAWTCILLAAGSAICAAAVNGILIPR
                     EFVSGGFTGLALVLHYLWPLLPVSVLYFLFNVPLFVASWFFVGRRFFAYSVVGMAVYA
                     VTLDWVRLPIPVTDPLLSALLAGILMGIGMGIILRSTGSAGGTDILSVILYQRWSVRP
                     GATILASNLLILILSLIFFSLEIVLYTLIYLYVSSQIIDIVVMGLSRRKVVFVISREW
                     EAISRRVLKEIDRGLTILHGEGGYSGHRQQVLYTVVTFRELGLLKRIVREADPAAFVV
                     VTDTQEVMGPRIGNQPPW"
     gene            43362..44408
                     /locus_tag="PLW16_00665"
     CDS             43362..44408
                     /locus_tag="PLW16_00665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB69445.1"
                     /translation="MSVTENKVMTAKEAIGRFVKDGDHLVIGNYTVGTCAALVFEVIR
                     QQRKGLTLYSQSGVFDVEVLVGGGCVDRLVSTYVYRAGGKEGGSAVERAMKAGTLEME
                     DYTNFQYNARLVAGMHGFSFMQVLEGAMVTDLFNKRSFLGDDKYRVIPCPYTGKQILT
                     VPAANPDVCIVHVQRADRFGNAQYWGALGSVAAAALASKRIVVSCEEIVEHDIIQSSP
                     HLTVIPGYRVDAVVEIPFGAHPTEVLGYYNLDRLMYALFFMSDMNGDGLKAWMDEWVY
                     GCVDRHAYLDHYMAKHGAESLDAIRAKAYYSAPANYGAAYTSCWDNEDRERSMGVTLA
                     EMERILEERGLLNG"
     gene            44401..45222
                     /locus_tag="PLW16_00670"
     CDS             44401..45222
                     /locus_tag="PLW16_00670"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB69446.1"
                     /translation="MAKKYTLDELLIITAAREIKDGENVILGVGLPTTAGAMAKALHA
                     PHATLMMESGILDIEPLVPPNHIADAHSCRGFSYATDLFSMFTMTYRGYVDVCFLGVA
                     QIDRFGNINTTVIGDYYQPKMRLPGSGGAADFLSYAKKTILTMRGGEFVNTLDYFTSP
                     GYLDGGDSRDASGLFPRGSGPVMLLTTKGVFRFDEQTKELVLAKVHPGISVDDIRKDI
                     PWDLKIAPDLAETDRPTDVEIDFIRRFAPTEAVGRKLMYELAIAHSFNKALARGK"
     gene            complement(46568..47872)
                     /locus_tag="PLW16_00675"
     CDS             complement(46568..47872)
                     /locus_tag="PLW16_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPB69447.1"
                     /translation="MNFIKHDVWRIRRTNLPPGKSFFLTLLRVLILSVRGFNEDKCEL
                     RASALTFYSLISIVPVAAMAFGIAKGFGFEKVLEAQLRSKLTGHEEILNNVIQFSHSL
                     LESTKGGLLAGIGLIVLFWAVIKVLGQIEESLNDIWGIKEQRSLGRKFGDYLSLMLVC
                     PILIILSSSVTIFVTTQVTLIMERITILGAFSPVVLFLMKLLPYSLLWGLFTFLYMFM
                     PNTKVRFTSALLAGVVSGTIFQVVQWAYIAFQIGVAKYNAIYGSFAAFPLFLIWLQLS
                     WLIVLYGAEVSFAHQNVNTYEFEPDALQVSRRLKKLLSLQIAQHIIQNFARGEAPPTA
                     RKVSHELEIPIRLVNDILFDLTQSGILSATDIGGDCERGFQPARDIGVLTIQHVVDAM
                     EQRGINTIPFAKTHEFSMISETLETFRKTIEELPANRLLKDL"
     gene            complement(48276..49010)
                     /locus_tag="PLW16_00680"
     CDS             complement(48276..49010)
                     /locus_tag="PLW16_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPB69448.1"
                     /translation="MKKIGSVFFILFWVAGIPGLAEAGIWSDILKELPVAKSAETASV
                     PDQKTAVSGLREALVIGTENAVKALSKTDGYFSNPLVKIKLPDNIQRVADVVARLGYQ
                     EQVDGLVLSMNRAAEAAAPKAIALFADAIKTLSIDDARKIIQGGDTAATQYFKDKTSE
                     RLYDEFKPVVVSNMNKVGVAKAYKDMIAPYETLPFVSKEAMDLDHYVTNKALDGLFLV
                     VAQEEQKIRTNPEARVTELLKTVFGN"
     gene            complement(49097..49414)
                     /locus_tag="PLW16_00685"
     CDS             complement(49097..49414)
                     /locus_tag="PLW16_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB69449.1"
                     /translation="MSETFFEEAREIKVFACVDVLVCGGGTSGIFAALASSRQGGGTA
                     TSYLVNPIPVMMGKGGLIQEFMDRMADMGGYIKQDPSTVSTCRRCLPEVRSKRDFQRT
                     TCR"
     gene            complement(49714..50979)
                     /locus_tag="PLW16_00690"
     CDS             complement(49714..50979)
                     /locus_tag="PLW16_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPB69450.1"
                     /translation="MTTVKRAKRSSDYPMLSIREAWLRVSSALEPLPPVRKPLVGAAG
                     LVLAEDIIAKDSMPPFAAASMDGYAVISSDPSLEKSITGEQDAGVKLDLSVEPGTTVR
                     IMTGAPLPTGADAVVPFEETGENEGIVRLKVIPKPGENVRPVGLDMAAGETVLPKGLV
                     LGPAEIGLLAALGQFQVWVHPRPRISIMSTGNELVAADETLRPGQIRDSNSLTLSVAA
                     AACGFEVTAASCPVRDDSGKLELALVEALNNSDAVITSGGVSMGKLDLVKPLLAKLGD
                     VLIGRVAMKPGKPFTFALLQGKPVFGLPGFPVSSLVCFEQFVRPALRLMSGLNLLWRP
                     CVEVSLAENLRHDPDRTEFQRAVVKADHDGFIAYSTGSQSSGRLKSLVGANALLILPI
                     GTGDAPVGSRVKAFLIDQPETEEELPISH"
     gene            complement(51003..51212)
                     /locus_tag="PLW16_00695"
     CDS             complement(51003..51212)
                     /locus_tag="PLW16_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPB69451.1"
                     /translation="MKISARNVLKGKVIEVSEGTVNTEVLLELAGGEKVVSMITKDSA
                     KRLGLKTGKKAYAIIKASSVMIGVD"
     gene            complement(51514..52233)
                     /locus_tag="PLW16_00700"
     CDS             complement(51514..52233)
                     /locus_tag="PLW16_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB69452.1"
                     /translation="MHLENVTIHPDKYPLRDRYPFNLPVFSQPQSIPFTTTVTLFVGE
                     NGTGKSTLLEALAQSCGIHIWRDESRSRFEYNPYEDQFHRCLSVTWTSGRVPGSYFGS
                     AIFHHFAQILDEWAAADPVQLQYFGGKSLLTQSHGQSLLSFFRSRYRLKGLYLLDEPE
                     TALSPRSQIDLLEILTNADDGRDAQFIIATHSPLLLACPGAVIYSFDAAPIETVAYEE
                     TEHYRIYKSFMENRRRFLRPR"
     gene            complement(52410..53165)
                     /gene="cobS"
                     /locus_tag="PLW16_00705"
     CDS             complement(52410..53165)
                     /gene="cobS"
                     /locus_tag="PLW16_00705"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPB69453.1"
                     /translation="MKAFFAALQFLTVLRVPLRFQGAGVDLERCAPFFPLVGLLVGAL
                     AALFDFLVSQILPSAIASTLTVLILVKLTGGLHMDGLADTADGFFSARDRETILAIMR
                     DSRIGTMGVMAIVFVILIKVGALTTLELPRRTALILLAPVAGRVALLVMMTALPYTRP
                     EGGLASRFLSGRSRANAVWGACFLVIAGGLLARWMGFAAAVLALAATAAVSAYSRRKI
                     GGFTGDTLGAACEIAESTVLLTAAAWAYGFPPF"
     gene            complement(53162..54226)
                     /gene="cobT"
                     /locus_tag="PLW16_00710"
     CDS             complement(53162..54226)
                     /gene="cobT"
                     /locus_tag="PLW16_00710"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPB69454.1"
                     /translation="MNLLETVVNSIPPADAGARACAHARLEQLSMPHWALGRLMDLAE
                     DLAGITGSMPPPVARKTIVTMAADHGVAVRGVSRYPQEVTVQMVRNFVRGGAGINALA
                     RLAGAEVVVVDLGVAGDLSDLADAGRILSRRIAAGTRDMTAGPAMSREQARTALEAGI
                     AVARDLGPTTDIFGTGEMGIGNTAPSSAIVALYSGRPVADVTGRGTGIDDAGLRRKIA
                     LIERALAVNAPDPADPVGVLAAVGGFEIGGIAGLILGAAAQRKPVLVDGFISTAGAIL
                     AARIAPRSLDAMIASHRSLEPGHRIALDCLGRRPLLDLDLRLGEGTGAALALHLVEAA
                     VRVLTDVATFDEANVSKAES"
     gene            complement(54223..54870)
                     /gene="cobU"
                     /locus_tag="PLW16_00715"
     CDS             complement(54223..54870)
                     /gene="cobU"
                     /locus_tag="PLW16_00715"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPB69455.1"
                     /translation="MARMILVTGGGRSGKSAYAQTTAEATAGPRTFIATCPVLDDEMA
                     ARIRRHREARSGAAWTTIEEEFDLTGALLQWGGDGVALVDCLTLWVNNLLYVAERQGR
                     AVSEDDIADRCRDIIRTCETLSGTVLFVTNEIGLGIVPDTPLARLYRDLAGRCNQVMA
                     QAAETVVLMVSGLPLFLKGETCIGGSREILRGQPAFSPGSASGHRSPSRMKKERG"
     gene            complement(54870..55661)
                     /gene="cbiR"
                     /locus_tag="PLW16_00720"
     CDS             complement(54870..55661)
                     /gene="cbiR"
                     /locus_tag="PLW16_00720"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HPB69456.1"
                     /translation="MDKGWPDLKGCFPFRLGTTSYILPAPILPNVRFLAPLVDEIELV
                     LFESRFAENLPTEAEIRELARLGDAYDLSYNVHLPTDLALGNPDAAVRRAAGETLWKF
                     YDRTLPLAPTAYILHLEGQPAAGNGSPAMDRWRENCLSSLAALFDTGVDRRRLAVENL
                     EAPFAAVAPIVAALELNVCLDAGHLLRQSLPMADLFTPYGNKTVMIHLHGIDGGCDHR
                     SLKDVPEAAWSFLRGFLETYRGGVSVEVFSRDDLGDSLVRLAELC"
     gene            55894..56793
                     /locus_tag="PLW16_00725"
     CDS             55894..56793
                     /locus_tag="PLW16_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPB69457.1"
                     /translation="MIPRKILPGTLIGFALLFLCMHAAAADPPRRIVSLAPNLTEILF
                     DLGLADRIAGVTNYCLLPPAARGKPKVGGFVNPSLEAIIALRPDLVLLTDDGNPRAVA
                     DRLRALGIRTHVFKTGHIRDLPREIRSLGDVLAVPGRARERAETMARTFERIENRART
                     RGAPRMRTALFIIQPEPLIVAGKGTTMDDALALLGLVNIGAAGRAAYPKLSLESVLSG
                     NPDVIFIARGHDDVMASADRLLKRLRSLDAVLRGRVYTVGDGILRPGPRLVDALEEMS
                     GHLQGRGAGAGADRIRRSARGGP"
     gene            complement(56765..57283)
                     /locus_tag="PLW16_00730"
     CDS             complement(56765..57283)
                     /locus_tag="PLW16_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPB69458.1"
                     /translation="MALEDIAVHDWSVRIHHLWEKQWLVLTAGDFAAGRYNAMTVAWG
                     SMGCMWKVPFVQVVVRPTRHTHAFMERYESFTLCAFPEAYRSAVLLIGTKSGRDLDKI
                     AASGLTPVPASAVAAPAYAEAELVVECRKMYWQDMDPGHFLDPSIDGNYPHKDYHRIY
                     FGRVMALRGRTA"
     gene            57537..58892
                     /gene="nhaA"
                     /locus_tag="PLW16_00735"
     CDS             57537..58892
                     /gene="nhaA"
                     /locus_tag="PLW16_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPB69459.1"
                     /translation="MDNAYPLEPLFGKILSPFERFLRQTTAGGIVLMGMTLLTLIVAS
                     SPWGDAYRHLWETPLRVGLGPQTLEMSLHTWVNEGLMALFFLLVGLELKREIMVGELA
                     SLRNAALPVAGAVGGMIAPAGIYILLNPSGPAAAGWGIPMATDIAFAVGILVLLAWRI
                     PRGVIIFLTALAIADDLGAVLVIALFYTQDIDMHLLAAAAALLGILVLLNRGGIRHSL
                     PYAILGILLWLALLKSGIHATLAGVLLAFAIPARPIFTPHQFDKRLDAMKQALHETEP
                     DSEGSDILRSLARMFGLAERLERTSAAVQSPQQRMEHRLAPWVTFGVIPLFAMANAGL
                     DVAAMPIREVVLQPVTLGVVLGLVLGKFLGITGFAWLAVKLGWARLPGGVGWRHISGS
                     AWLGGIGFTMSLFIGQLAFAWDPLLFEQARFGIMLASLLSAILGLGWLYFGARGAPAE
                     D"
     gene            complement(58889..60097)
                     /locus_tag="PLW16_00740"
     CDS             complement(58889..60097)
                     /locus_tag="PLW16_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPB69460.1"
                     /translation="MADFYQHGMITTLQRLGKRPLAELETDLQAVARMRNIALLLPAL
                     VSEFDTPSMPRIIDELKGVRYLRRLVLSLDRANRRQFSKVKKALSVLPMEVRVVWHDG
                     PRMKALYKELRTCDFSIDTPGKGRSVWMTLGYLLADKNLYAIALHDTDIVNYSREMLA
                     RLIYPVVHPAVDFEFSKGYYARVTDRLYGRVTRLYYIPLIRTLKRILEYNAFLEYLGN
                     FRYALSGEFALVSSLARGIRISPTWGLEVSLLSEVYQRASLNRICQVEISETYEHKHQ
                     EMDRGKPDAGLVRMAKDIAEALIRVLSQDGIVMSQAFFRTLYTAYIEEARVTIEKYHA
                     LSLLNGLYYDRHSEIEATEIFVVALREATKAFVADPVGIPMMSAWVRVEAAIPDFGDR
                     LTEAVEADNR"
     gene            complement(60070..61350)
                     /locus_tag="PLW16_00745"
     CDS             complement(60070..61350)
                     /locus_tag="PLW16_00745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB69461.1"
                     /translation="MQKFSSAIRDHVRQKIEGLRQADIVIGIPAYQSGPAIVHVIRTV
                     AQGLKDHYPDARALLMIADGGSTDDTRDLARSYDVKSFNIEKIVTIYRGLPGKGSALR
                     AVFEVAAFLKPKAMAVFDSDLASITPNWVRNLLEPVFGGYDFVVPDYQRHKLDGTITN
                     TIAYNLTRALYGQRIRQPIGGDFGISLPLVRDYLQREVWETDVARFGIDIWMTTTAIV
                     SGYRICQARLGSKIHGEKDPAADLGPMFRQVVGTIFQLMEDYEPHWRQVQASTSVPTL
                     GDYVGEEPPALSIDQESLVDYFRIGFRNFGGVWHKILERRDLDIVKHLARHADAGDFT
                     VPVETWVRIVYRYMRAFHTTPRQRMKLLDTLIPLYYGRVASLVNELRDKDAAGAEEHF
                     EQQARMFESMKGYLLDLWEQTEGGEPWQTSTSTA"
     gene            complement(61381..63072)
                     /locus_tag="PLW16_00750"
     CDS             complement(61381..63072)
                     /locus_tag="PLW16_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPB69462.1"
                     /translation="MTSQKRITGRFILGLSFLIFLISALCVVPTGDARQTAPKEGKDV
                     QAPGAPVVFDNRTLFSIQARVFSLSPEERAKFVQARVAKLADNHQLNLEKISVTNGET
                     SSDVSVDDLVLMTVTDADAKAAGKKRLDLAQEHAATIQAAIGEYRKDRSMKSILTGTL
                     FAVLFTALLCLFVCAWKKLFVKVYAAIESWKGVRIPTIKFQKLEILSSQQLGNMLIRA
                     ARLIYWVILLSILYFYTGFVLTFFPWTKAYSAKILGYILTPLSIIANALVNFLPNLFF
                     LTIIVLILYYLLKLAKFIFSAVERGVLVFPGFYPEWAQSTYAIVRFFLLAFGAVAAFP
                     YLPGSDAPAFKGVSIFIGLLVSLGSTAAVANVVAGLIMTYMRAFRIGDRVRISDATGD
                     VVEKTLLVTRLRTIKNEEITIPNAMILGSHIVNYSSAAGTTGLILHTGVTIGYDVPWR
                     RVHECLTAAAAKTVHILKAPPPFVFQTSLDDFYVSYELNAYTDKPAMMASIYSELHQN
                     IQDTFNEAGIEIMSPHYAALREGNQTTIPEAYLPESYTPPVFRFVGPEGKTGGNP"
     gene            complement(63232..64095)
                     /locus_tag="PLW16_00755"
     CDS             complement(63232..64095)
                     /locus_tag="PLW16_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPB69463.1"
                     /translation="MNRKPTLIIFTDLDGTLLNEADYAWTDANSALTAIREHGIPLIF
                     TTSKTRPEVERLQAAMDIRDPFICENGAAVFFPEGYRRFSIPHAVLQGRYCVLSRGVP
                     YTEIRRFVAEARACYDILGFGDLSPREIARLTGLPLAEARLAKAREFSEPLIIENPEH
                     VAALRKMARTRGFSLIRGGRFHHLSGGGQDKGRAVRLATKVFRRNLGRTPLTIGLGNS
                     ENDAPLLDAVDIPVLIPHAGGGGIAGGFPNARRANHPGSRGWNEAVLALLDETGALPV
                     RGRKQNADRRK"
     gene            64383..65120
                     /locus_tag="PLW16_00760"
     CDS             64383..65120
                     /locus_tag="PLW16_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69464.1"
                     /translation="MKKTTNRLLTGFGIVLVLVILALGGYLVKMNVETRKMTPSATGE
                     ITAGVYAVRDSYVNLFLIKGADGYIAVDAGNSLERVRQEMQTLSIDPSQVTAVFLTHT
                     DFDHVAALELFPRATIYLSKAEEQMINGTTPRFAVIHNRKLPRYALLDDGQTVDVSGR
                     SVRGIATPGHTPGAMSYVVDGAYLFVGDTMSLKNGKADLFNEFFNMDSTTQEASIRKL
                     AALKNPKFVFTAHYGFTDDAARAFGDR"
     gene            65255..66703
                     /locus_tag="PLW16_00765"
     CDS             65255..66703
                     /locus_tag="PLW16_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB69465.1"
                     /translation="MQDFTEGHIGRQVINFSTPIMFGNLLLSVYGIINMIWVGRLLGH
                     QAVAAISASLPIVMLMPAFLIGLGMATNVLIAQAFGRRDTAMLKKILANSFIASMTFC
                     LLISVIALLFRRQLLDWVNAPREIQGMALSYLTIIMATLVFQFFINWMNGMLRGLGDA
                     TTVVKILILLAVFNVVFVPLFIRGIGPLPPLGVAGAAWGTALAAVSTCVIGYLYLLRF
                     NPYVNMQNWDYRLDWRIIREVFVIGVPASLQMMVVSLAGVIIISLVNRYGTAVTAAFG
                     IGMQIDMLATIPFMSIGMAVTTIAGHNLGARKLDRVFQTLRVSVWFGLLVAFACTVVL
                     LIFPHDIGAIFLKESLEKARVLDTVRDYYRWMAFIFPCFAVIFAIQGVLRSAGDTLAL
                     MGLSFIAMFVIRIPLAYFLAGPIGLKQDGIWMAMLSSSALAVFLNWLYFRYGPWKKRR
                     ILGDQGKGLPEERVEVPGRAPISARNRASTRR"
     gene            complement(66666..68117)
                     /locus_tag="PLW16_00770"
     CDS             complement(66666..68117)
                     /locus_tag="PLW16_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPB69466.1"
                     /translation="MNFRLILYLVAVLVFYIGLSMTAPLLVALYYGDGGAHAFLLSLI
                     ATCGLGGGALLWSRGQEDYALNHRDGVAIVALGWISAGLVATLPYLLSGTIPDFTDAY
                     FECISGFTTTGASILTDVERLPRGILFWRAQTQWLGGMGIIVLSIAILPFLGVGGMQL
                     YKAETPSPVVDKLTPRISDTARTLWKIYLFLTVMQIALLLFGGLSWFDSVCHAFATLP
                     TGGFSTKNASVGHYHSAYVECIVIAFMFIAGINFSLHYKLVHGRLREFIRDPEFRAYL
                     LITGTFILIVVLDVYGGKIYPTVFEAFRHATFSVVSLLTTTGFVTADFEAWPALSQQV
                     LLLCMFLGGMAGSTGGGIKILRVVLLAKHCYLEILRIIHPHAVTVVKLGRTPVPQTVM
                     RSIWGFFMLYVGIFILAAMALAALGMDVMSSISGAAACLGNVGPGLGSIGPMDNFSGV
                     PPAGKWILIACMLLGRLEIYTIIVLLMPGFWRK"
     gene            complement(68117..69475)
                     /gene="trkA"
                     /locus_tag="PLW16_00775"
     CDS             complement(68117..69475)
                     /gene="trkA"
                     /locus_tag="PLW16_00775"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPB69467.1"
                     /translation="MRVIIIGAGEVGYHIAKKLSDESQEVVLIDQDPAKIRRISENLD
                     VQAFLGSGTSPQTLRAAGIQEAELLAAATDSDEVNLIACLLARSLNPHMVKVARVKNR
                     EYLGEETLFSRDLLGVDLIINTNAMMVDTILGLMEVPGASEVIDFVGGEVKLVSFLIT
                     TESPLAGKRLTDLKAYEGNLLVGAIVRGHKILIPSGRDTIQPMDLVYLVMKRAEVNPT
                     LDRLGISTRPLKNVLIVGAGETGSAVAKALDRRRMNVKIIDQDGEKCKKLATVLDRAI
                     VIRGDGADRDLLREEYAGDMDLLIALTGDEESNVLISLLAKELGTRRTITRVSKLSYM
                     HIVSAIGLDTVINPRMAAVRAILQYIRRGRIISVAPLREEEAEAIEAEALETSDIVNT
                     PLSKVPFPPGAILGAIVRGNEIIIPSGDTIIRPHDRLIIFALQRVSKKIEELLTVKLE
                     YF"
     gene            69865..70341
                     /locus_tag="PLW16_00780"
     CDS             69865..70341
                     /locus_tag="PLW16_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPB69468.1"
                     /translation="MSGALISEQMRAAVGSPLASGQPFAVEKGSIRDLAEAINHLHPL
                     YVDEAYAQSTGHGSVLAPPTYPSYNLALGAPLEVLTFEFPVGSILHGSDDWEFLGNIH
                     AGDILTPRGKLLDLYEKQGKRGGMLFIVSEITYTNQRGEVVAIYRPTEVVIALPTA"
     gene            70356..70817
                     /locus_tag="PLW16_00785"
     CDS             70356..70817
                     /locus_tag="PLW16_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB69469.1"
                     /translation="MKTQPYFEDIRVGEEIPHQVREPLSTRGLVKWAAAVRDFYEVHY
                     DKDFARSIGLRDVIAHGPNKCALLGRLMTEWIGEAGRLHRLSCTHKASNFPGETLVCR
                     GRVKDKYSKDGRHYVECEVWVENQDGVVAAPGAATVSLPSRGVEPLPSARP"
     regulatory      70839..71028
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            71041..72075
                     /locus_tag="PLW16_00790"
     CDS             71041..72075
                     /locus_tag="PLW16_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB69470.1"
                     /translation="MRPTVHPIVTPFRVLRISGILFLICLAVFVGSLFAGPGQVGVRT
                     LLASLLSTGSGSFAGDADAGQTILLSLRLPRLVFAGIVGAALSVSGVVFQALLRNPLA
                     DPYILGISGGAAVGAIAGILLGAAAVPFGVPGLAFVGALTTLLAVFAVAGAGRDLRSH
                     TLLLAGVIVNAFFNAVIMFLLAVSSSGDLHSIMFWLMGDLSLARGRDILSVGAALVLG
                     FSVICLYARPLNLIVTGEETARTLGVDPATAKRVLLVVTSLMTGLAVSVSGTIGFVGL
                     MVPHLMRMAFGPDHRLLLPASLFFGAAFLILADTLARTLLAPMELPVGVVTALCGAPY
                     FIYLLRRMND"
     gene            72125..72883
                     /locus_tag="PLW16_00795"
     CDS             72125..72883
                     /locus_tag="PLW16_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69471.1"
                     /translation="MLTDVAFSVAAGAFLGIIGPNGSGKTTLLKVMNGLLRPQAGAVQ
                     LNGRPLSGMRRREVARVMAVVPQDAALLFTFTVEEMVLMGRSPHLGRFGFEGKGDFRI
                     ARAAMDRTGVLALAHRPYDALSGGERQRVLIARALAQEPRILLLDEPTAYLDIRHQAG
                     IFDLMKDLNRRRGLTVVAVTHDINLASLYSDEVLLLADGRVFGAGPPEAVVTTENIRG
                     AYGISVLVDPHPATGLPRVTPAGYAGGGGPDPAP"
     regulatory      73114..73234
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            73286..75394
                     /locus_tag="PLW16_00800"
     CDS             73286..75394
                     /locus_tag="PLW16_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB69472.1"
                     /translation="MRIRLQWLVMLFCLSGAMLTPAPGLSAAATAEPPGAAITLEEVV
                     VTATKTPAKRRDVPNAVLVLDAGEIRASGAKTIGELLANEPGIDWQTYGNYGGAAQEI
                     HIRGMRGNGTQVLINGVPVNSPSLGVADVGRLPIDAVERIEIVKGSGSLLYGSGAMGG
                     TVHILTKGPLRDKTDLKIGAGYGSRNTWRVAAESGMFVAGDFGYYLTAGRTETDGFRD
                     NSDLRQTDASLTLVLDRKDGMNVRLYGDYLYRKYGMPGVEPPAGTGTYAIGTETFYNR
                     DAATLLDRSGDKDGRIVLEVKGKPAPWLGYDFKGYYTDMENHNVQRYAFNGSGDENWV
                     TNRVLGADGHVDVYPFEGAKLLLGGEYKNFIWKNDNFDLDTSGRRTVETAHDAGLHTK
                     GVFTEAEYRPSRYWKMLAGFRHENHSAFGSGNLPLFGLIVNLGEATALKINHGKHFLA
                     PTPNDLYWPADPYTRGNVDLKPETGWHTDVTVEQSLLNDKVFVSLSYFHWKVEDKIQW
                     EPDSRGVWSPMNLADYRAEGLEAGVRIGPFDGLTLALGYTYLDAEEENRAYTRQDYGW
                     PPFIAPDFRYAMVPRRAAMTPEHQFKGDLTYRSRFGLTATATVRYVSDRLVYNTETTT
                     YPDTETVVHTLRSYWTADLKLEQRLGGHWVLTLAGRNLFDRDYDTRLGSFTDQTTLKS
                     ALCGYPGAGRSVFAGVAYEF"
     gene            complement(75484..77604)
                     /locus_tag="PLW16_00805"
     CDS             complement(75484..77604)
                     /locus_tag="PLW16_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPB69473.1"
                     /translation="MPSSGMGDPRAAACPPPNRLSGEKSPYLLQHATNPVDWRPWGEE
                     AFAEAARQDKPIFLSIGYSTCHWCHVMAHESFEDPAVAALMNRHFINIKVDREERPDI
                     DGIYMAACQMMTGRGGWPLTIIMTADKRPFYAATYIPRQDRFGQIGMLALLPRIDEIW
                     RNRRQDIVDKAEEVVAALRAGREAPPGAAPRTESLDAAFDQLARLFDGTNGGFGSRPK
                     FPTPQNLLFLLRYWKRTGNRYALGMAEATLQAMRRGGIYDQIGFGFHRYSVDPHWLVP
                     HFEKMLYDQAMLAYAYTEAFQATGEKTYADTAREVFSYVLTRMTSPEGAFYSAEDADS
                     EGEEGKFYLWRWEDVDGILDEADADLFRRTFRFEKTGNFTDELTGRKTGMNIPHLAPA
                     TEGAGGTSPIIDPESEARVERARQTLFAVRQRRVPPQKDDKILTDWNGLMIASLAKAG
                     ACLDEPAYIDAAQRAAAFILGRMRRPDGALLHRFRDGEAGIAGTLDDYAFFTLALIEL
                     YEANGESSYLEEALALTCVLMTAFKDPRGGFFFTPRDGEEILVRQKEIFEGAMPSGNA
                     VAVWNLLRLGRMTGETTLLDEAWDAAKAFDRDLRQSPGAYAQFMVALDFAFGPAREVV
                     VVGDPAAEDTRALLRALRSRFLPRTVVILRPAGEASPPICRLAPYTRDMPCIDGRATA
                     YVCTGNACLKPTTDLRTMLAAIDA"
     gene            77701..78138
                     /locus_tag="PLW16_00810"
     CDS             77701..78138
                     /locus_tag="PLW16_00810"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB69474.1"
                     /translation="MIEKMTSLVREKDTCVLATAAGGEPHCSLMSYVADDDGLSLYML
                     SRKGTRKHGNLMNNARVSLLIDTREDIAGRDRGAVRALTVRGVFQSIDDDSRRGAILE
                     KLLDRHPPLRELAGHPDIDVFEVKAVSFELLEGVSRATVVEVA"
     gene            complement(78048..79061)
                     /locus_tag="PLW16_00815"
     CDS             complement(78048..79061)
                     /locus_tag="PLW16_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HPB69475.1"
                     /translation="MTDLIGDLAAEQETLEAVVTALAEAVWDRPTPFYGWTIRDEICH
                     LAFFDAAARLSATDAAAFAAQMDQASRDGDPFEETLKIGRALSTEALLDWWRRERSGL
                     IDGLKALPPGHRLPWFGPPMGVRSFTTARLMETWAHGQDILDALGQERPATIRLRHIA
                     HLGVSTRDWSFLIRGLPPPATPVRVELLGPSGEVWTWGAADAGEAIRGAAEDFCLVVT
                     QRRHVDDTDLNVAGDGARHWMRIAQAFAGPPAEGPEPGAFPRRGTAAAPGPRPAEPGA
                     DRSERSSRTGSAFPSPGRGMGQADRRAARAGGQATSTTVARETPSSSSKETAFTSKTS
                     ISG"
     gene            complement(79126..80529)
                     /locus_tag="PLW16_00820"
     CDS             complement(79126..80529)
                     /locus_tag="PLW16_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB69476.1"
                     /translation="MTKPYDTIVVGAGIAGLGVGAILTREAGQKVLVLDRFPKFGGRM
                     MSYDGYPSAGWRVEIGLHMIELGEKSTCNALNERVGKKVAWAPFSDTVDIWNGEKFVS
                     VAELVPMTREDKAAFRDLMQKIAMLSDAEIEAWDDRSLAEWLHENVPQAPVRELFTDL
                     GMIMTTIPHAINMAAGEVLYIARENLLKTRQALQASYPRDGMEGVTRGLAEVIREGGG
                     EIRLNCDVQEVGVADGRAAGVRVPAQKHIYQEEYRIFETELLTAERVVCALPIYKLSQ
                     ILDFNPVTSPLPRWWVKRITDIQHEITGLIGFMIGLKEPVVDPRKKCFFSALKTRHAG
                     FPFQAFPASNFSEHVAPAGKQVIHTDLVCEHAEASDKFMRQRLLKRHWEDLKDMFPGI
                     EAKTEWRLPYYVDGCDGLARQPGLVGNFKPGLKAPGIPNLYFAGDTYRGRGLAINSAA
                     QSGMLCADLILKELKGK"
     gene            80910..82517
                     /locus_tag="PLW16_00825"
     CDS             80910..82517
                     /locus_tag="PLW16_00825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69477.1"
                     /translation="MKIKFMGAARTVTGSCFIVEAAGHRFAVDCGMHQGNAEIDQRNW
                     EVDLYDPAGIEFFIITHAHIDHSGLIPRMVQKGFHGPIYMTPPTKDLLQVMLLDSAHI
                     QETEAQWKTKRRLRHGDKRIQPLYTQKDAQASFPLFESVPYEKVFDPCDGVRVNLRDA
                     GHILGAALAELRIRDNGADTKLVFSGDLGRPSQLMMKDPSVVEEADILFMESTYGNRN
                     HKNEDASLDELAQAVAYSHERGEKVIIPAFAVERTQEMIYSLHLLEKSGRLPKDMPVF
                     VDSPLAIAATEIFRRHKHQFDAEAQALLARGEDPLTLPGLRYTQSTQDSIDINAVAGP
                     AVVISASGMANAGRIKHHLRHNLWREGASIVFVGFQAAGTTGRRIIDGAEKVRLFGED
                     VAVKAKVFTINGFSAHAGQTQLLDWFAHFRNPAMQLFLVHGEYTAQQALAGLIEERFG
                     IKAVIPDYLEETTLNRGEALKRMEFPEQAAPRIDWEYLIADVEAKLAHLKERQANIEG
                     KARVEQTDLRDRLLEVNRSLMQLISES"
     gene            82542..83756
                     /locus_tag="PLW16_00830"
     CDS             82542..83756
                     /locus_tag="PLW16_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB69478.1"
                     /translation="MAEDLSRLKIDKSAQALRPARRRAFLLPVLILLVAVILAALYVL
                     GVLKPASAVQTATVSFVYPSQNFTVLNASGYIVAQRKAAVASKATGRLTSLWVEEGSR
                     VTRGRVIAQLESDDAEASRDQAAANLSVSRASLNQAKTVLEDAARDYDRNRRLLEKGI
                     VARNAFDLAETRYRSAQAAVTGAEAAIRAAAAGLQGANVALEYTRIRAPFDAVVLTKN
                     ADIGDIVTPLGAAANAKAAVVTIADLGSLVVEVDVSEANIGRVTVGQPCEIQLDAIPD
                     ERFPGRVHMIVPTVDRSKAAVMVKVRFLSADPRRLPDMSAKVAFLTRPVGPDEGKPRL
                     GVPRAAVVKRGGRQVVYVVQGGRVRETDIRTGRGLGEMIEVAGGPKAGEKVVVNPPAA
                     LGDGGRIEVAEP"
     gene            83753..84466
                     /locus_tag="PLW16_00835"
     CDS             83753..84466
                     /locus_tag="PLW16_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69479.1"
                     /translation="MTTEAPAPIIDVQGVSKSYRRGELVVPVLHDITFTIAPGEFLSL
                     MGPSGSGKSTLLNLVAGIDHVDGGRIIVEGVDITRLTETELARWRANSVGFIFQFYNL
                     IPVLTALENVELPLHLSGLSRRARREHALTALDLVGLSHRLDHFPGQLSGGEQQRVAI
                     ARAVVTDPTVLVADEPTGDLDRVSAGEVLDMMARLNRELGKTIVMVTHDPRAAARAHL
                     KRHLDKGVLSADDPSENPD"
     gene            84441..85598
                     /locus_tag="PLW16_00840"
     CDS             84441..85598
                     /locus_tag="PLW16_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB69480.1"
                     /translation="MILLRILIRNAFRNRLRTGLTILGITIAILAFGLLRTVVSAWYA
                     GVAASSATRLVTRNAVSLIFPLPLAYKDKIRQVDGVSHVSWGNWFGGIYISEKNFFAN
                     FCVDPRSYLALYPEYILSDRDRKAFLSDRKGFIAGRKLADRFGWKPGDTVTLKGVIFP
                     GNWDFVLRGVYRGRDRTIDETQFLFQWDYLNETVRQRYPSRADQVGFYMIGVASPERA
                     ADVAGSIDRLFKNSLAETLTETEKAFQLGFIAMTEAIVMAIQLVSIVIIVIIMAVVAN
                     TMAMTTRERIGDYAILKTLGFGGRQIALLIFGEALVITGIGAAIGMALTYPAARAFGS
                     TLSNFFPVFNVARETLWLDLGAALVVAVAAAVIPTQRAIGIRIADGLRRIG"
     gene            85598..86764
                     /locus_tag="PLW16_00845"
     CDS             85598..86764
                     /locus_tag="PLW16_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB69481.1"
                     /translation="MAVPFSYSLRNLWTRRMTTILTVAGMALVVFVFATILMLAEGLE
                     RTLVETGSADNVVVIRKGSASEVMSGVERAQAAVVEVQPEVAVGRDGRRLLAKELVVL
                     IALPKRGNGKASNVVLRGVGPASLELRPQVRLVAGRLPRMGSSEVMAGRSVVKGFQGV
                     GLNERVCCGLRDWRVVGVFDAGNTGFSSEIWGDADQFMQAFRRPAYSSVLFKLRDARE
                     FPAVKARLETDPRLPLEAKRETRYYLDQSEIMAKFLRILGVSLTVIFSIGAMLGAMIT
                     MYSAVANRTAEIGTLRALGFRRRNILIAFLMESLLLGFIGGAVGIACSSLTQLLTIST
                     LNFQTFSELAFRFTLSPAIAAQAMGFSLGMGFIGGVLPALRASRLNIVDALRAV"
     gene            complement(86845..87957)
                     /locus_tag="PLW16_00850"
     CDS             complement(86845..87957)
                     /locus_tag="PLW16_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPB69482.1"
                     /translation="MSKRYEPLIPPHRILLGPGPSNVPPAVMAALGSPVIGHLDPAFL
                     AIMEEVQEMLRAVLRTENRFTLPVSGTGSAGMEAALVNVLEPGDRILVCVNGIFGERM
                     CDIAVRCGAELERVEAAWGSLVDPAAVRRALAAFPAKVVAVVHAETSTGVLQPLPELA
                     EAAHARGALFLVDAVTSLAGAPLETDAWGIDVCYSGTQKCLSCPPGLAPITFGPRAVE
                     VLEKRRTRVQSWYLDMTLILKYWGPDRVYHHTAPISMIYALREALRIVLEEGLEARWE
                     RHRRVHQALVRGLQKMGFSMLVAEADRAPMINAVLVPEGMDEAAVRRKLLVSAGIEIG
                     GGLGALKGKVWRIGLMGASCTEENVEALLAALAALR"
     gene            complement(87990..88982)
                     /locus_tag="PLW16_00855"
     CDS             complement(87990..88982)
                     /locus_tag="PLW16_00855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPB69483.1"
                     /translation="MGKAKVFVTRLIPEAGLGRIRTACDARIWEERFPPDRKVLFEQV
                     RGVDGILSLLNDRMDAAVMDAAGSGLKIISNYAVGFDNIDVPAATARGILVGHTPGAV
                     TEATADLAFALLMAAARRIVEGVDYIRAGKWETFEPLVLLGRDIHGAALGIVGMGRIG
                     QAMARRARGFDMEVLYYHPRGETAASEAAGAKFCATLDELLRKSDFVSLHVPLNAETR
                     HLIDGAALRNMKETAVLVNTARGGVVDPEALYEALRNGVIAAAALDVTDPEPLPEDHP
                     LLGLPNCLVVPHIGSASVATRERMAVMAADNLIAALRGEVPKHLVNPEALSRRG"
     gene            complement(88993..89910)
                     /locus_tag="PLW16_00860"
     CDS             complement(88993..89910)
                     /locus_tag="PLW16_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPB69484.1"
                     /translation="MNIAIVGIGGVGGYYGGRLARRYEAGDEHRIAFVARGAHLEAIR
                     NGGLKVVTPDGEFTARPAQATDRPGDLGPLDLVLITVKGYDLEGAAGALEAVTGEATV
                     VIPLENGVDTAERLGKILPRARVLNGTVYISSRIAAPGVVQQLGGAGSLFFGPETGPV
                     EPFRPIESLFLEAGIQAVLTAAVAVEVWKKFLFISPVAGTTALFGKSLGGILEEPEAL
                     RLLEGMMDEVDRLARARGIDLPANAVAQALETVARFPYETKTSFQVDVENGRKTEIET
                     FMGYVVRAGQALGIDTPFNRRVYDALSKS"
     gene            complement(89907..90590)
                     /locus_tag="PLW16_00865"
     CDS             complement(89907..90590)
                     /locus_tag="PLW16_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69485.1"
                     /translation="MFEHEHILREPLFYLFVVTTILLTVGYFQGRRRNRRIFLDAFQA
                     LVDTVKPDDQTYTTIGGVVGYHANLLLKRKGPVSRVDATITLLPRQSWLYLPISFLTM
                     RFDRLFVTIHLRSDVPGEGHLIERSYSRFRGPKIANDARLEKESVRWGKLDFILYYDN
                     LNVRERFRGHLARHPDPGTIRHIALVPGDRKGFVFMIPKRGRVATMFRPVYELIISLA
                     SDAKKGKSP"
     gene            complement(90593..91528)
                     /locus_tag="PLW16_00870"
     CDS             complement(90593..91528)
                     /locus_tag="PLW16_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HPB69486.1"
                     /translation="MKFLFFTGKGGVGKSTIAAAWGRQISRRARVLVQSLDPAHNLGD
                     IFGVTAGNGRKKISDTLTIQEVDLKKLSREYLERETRVLGETYRYLQVLNLDDYFSVL
                     KYSPGIEEYALLTSIERTLNAETGFDYVMFDTPPTGLTLRFLALPSVTLTWIDRLIRI
                     RETILEKRYTIGKIRRPLKPGETPAEERLAYDPGEDAILERLRALRRNYDRLNGLLRG
                     EDCSVVLVFNPDLLSLQESCRLAEGLAELRLPLRLLVNNKVTERNREMAERVEQALRK
                     AAPGVPVERVPLSEALYEGKETVLYRIENDIGERF"
     gene            complement(91525..91809)
                     /locus_tag="PLW16_00875"
     CDS             complement(91525..91809)
                     /locus_tag="PLW16_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69487.1"
                     /translation="MKFSPISLLREFLVRLDDHHRESAAALTAVEARELENIFALLVL
                     GSFIGLPAPPTFLAVELLPFMEREIRILNVRAEDAGDMLAEMCGMLGIDG"
     gene            complement(91879..93621)
                     /locus_tag="PLW16_00880"
     CDS             complement(91879..93621)
                     /locus_tag="PLW16_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPB69488.1"
                     /translation="MTTLIILVGLAIYVLLYFLYGKKLERDVVGATDANEAPSSRLYD
                     GVDYIPARKAVLFGHHFSSIAGAAPIIGPALAMAYGWVPGLLWVWFGNVFIGAVHDYL
                     ALMASVRYDGRSIQFVASDLMGKRTGTAFYWLVFFLLILVVAAFSAVVGGMFVKTPAI
                     ASAYVWSIVAALILGVLIYRVKMNFTLASVIGVIMLAVAIFLGVEFPMPATYETWMYI
                     LFFYIIIAAAIPVNVLLQPRDYQNSFLLYAGLILGLLAAVFAFRGFEVPAFTSFSPIL
                     TGGKPTPFWPAIPLIIACGSLSGFHSLVASGTTSKQLTKESDGLLIGYGAMFTEGFLS
                     TIVVVAIAGFGFTALQTAAAEKGIEFTLTASTWATQFTPLSSKLGLSQANLFIQSYAD
                     MVAATWLSVIPTQYVKVLAGMWVAAFALTTLDTTNRLARYCVSEMALPLKGTSAGLYN
                     FLTNRWIASLIPAFIGIYLAYSKNFTILWPSFGAANQLIASIALMTGACWVHSKLQSR
                     FCHVAVIPAWILWVTVTAALVWFMWVPLPANIASSPVQGWTVMGISIVMLIMNFIFIV
                     DFVKKYYREQVQSK"
     gene            94043..94822
                     /locus_tag="PLW16_00885"
     CDS             94043..94822
                     /locus_tag="PLW16_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB69489.1"
                     /translation="MSFGIGILAGALGALVGLGGGVVMIPLLTGWLGFSQHQAQGTSL
                     LALMFTGLAGAVAYGANRSVDWPAAALLVLPAMLMAWVGARSCNRLPERNLRRLFSAF
                     LIAISALMLLKPFLHPAASGAASVGFEAGVLVVTGLVTGFLSGLFGIGGGAIVIAAMV
                     LVLGYGQHPAQGSALVAMVPVAAVGAWCYRDFGRADAGVLKGLVPGILLGAAGGAQLA
                     HLFPDAALRIIFAVVLAGTGLQGLRAKVGEASCRASGGRAG"
     gene            94825..95193
                     /locus_tag="PLW16_00890"
     CDS             94825..95193
                     /locus_tag="PLW16_00890"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPB69490.1"
                     /translation="MSAKAFDRAVARAVDRIPAEIRDLMENVVVAVRKQPTQEMLDAV
                     GMTADDPPLGLYWGESLLERSSFSPVQYPDTIFIFQEPLEEMCETLAELEREIEITVV
                     HEVAHFLGMDEDRLADLGYA"
     gene            complement(95213..97489)
                     /locus_tag="PLW16_00895"
     CDS             complement(95213..97489)
                     /locus_tag="PLW16_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB69491.1"
                     /translation="MSDGSALSPTVEILIAVNTALTNLRLYPPTSAMIRTTLEKLHHT
                     LGQVLENDEEVTFAEAEQTLLVNGTPLGPKDRERPQVAAFLQLMMNVGAHNIVFSRGV
                     GADELLAFLDILTEKPEMLAERGGLRKVLEARDLPHIRLGEKRFVAMDPDRPFPGTGG
                     IGEESILDYLAGTAGAASLDPVRIREMAQDPEWFGRVLRAGIAQIAAGDASPPEGPFS
                     DRLGSMMRMLGETAAPEEAESLSLLIARTVVQLDAGVIQALLSRGTESGFEERFFQHV
                     RDLLHRNLPPAGGGRPTDPGAPSGDPALAEPLADADLPPGARAPRGRNTGIFERQVAA
                     LETMIKRHGQGEPILSVDGGPAATPVAAPAAAPKEAPAERIGLLYARGRGAEAEEIID
                     ELFATLASERAAEREQASDNLARILPGLVSDNRYEPVVRNLEKLTAWIGKETAATTAY
                     TKLCRQVGELGQRLIRERRFSACRAIVKTFHQIHTGALERNDTIQQLAEDVLRDLSQA
                     ELIDILFNVFQTRDRQGLDQPGQLFESDEAGRLLVRLEPAAAHFLELLGKSENAEERI
                     RIMNLINDMGTLAVSAVLTELRQDGVWYYQRNLARLLGRIGGDIHVRALQPLLLHADR
                     RVQKETLKSINGIGGDERGPLLTETLPQAQEALQLDIVPLLGSLRHPPAVPVLIDLLK
                     AKPVKSSAAREELEEKICAALGAIGEESALPALTSVQRQIGFLSLRAFSPRVKAAAGR
                     AIAEIDRKRKRGGGVGKR"
     gene            complement(97626..98174)
                     /locus_tag="PLW16_00900"
     CDS             complement(97626..98174)
                     /locus_tag="PLW16_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPB69492.1"
                     /translation="MTSKKLLVLAILASIFAHAVVLSLTGFIDMTGESRDPETLTVSL
                     KEPEKATEEQATKPAAPAARRAPAALPPVPQEDTVALENPDSPYTPYLLKVKYRINRR
                     WAYPPEAVSRGHEGETVVRFSIAANGSLAGADVVTSAGSELLDRAALDVIRTSSPFGP
                     LPRPFRLDRLHIVARFQYRLKE"
     gene            98332..>99562
                     /locus_tag="PLW16_00905"
     CDS             98332..>99562
                     /locus_tag="PLW16_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB69493.1"
                     /translation="MTLRRKILIIFGLTLLALIAILWLSSNSDLIVPLLLVGLVGFVL
                     LEKFALRPLAGLAGEIARIGECGDFSERISTQGRDNGAGMAAAVNRMLDALEKTRGQF
                     ETESARYRAIIELQSEFIGRFRADGTITFVNDAFCRLVGKPRDELTGTSLRTLIPTDD
                     YERILQQVRVLTPAAPDLPDNEHRIAFPDGRARWLLWNARAIFDAGGRFLECQAVGRD
                     ITREKELRSAYRRSVSTIEQAKIEWEGSVDSLEGLILLVDDQGRVVRANRSLEAWSLG
                     SVKAAIGRPLHEVLHPGCSESECALASFLKASLGRVRVGETVETELADPRFEKTVQVR
                     LRTVSLGGRRNGPASAAIAIVLVLDVTARVRAEETLKAVNSQLEEANRQLGMAYARMR
                     DEKDHLKRQLFQEEMGLL"
BASE COUNT        17778 a        30831 c        32321 g        18632 t
ORIGIN      
        1 cgatcgtcgg actggacgcc tcgccggagc ggatcgccca tgcgcaacgg tgctatggct
       61 cccgtgcggg gatcgaattc gccgtccacg acctgcgaga atccttaagc cgcttcggca
      121 cgtttgacgg cgcctgggtc cgtttcgtcc tggagtattt ccagcgggaa agccttgaga
      181 tcgtccggaa tattgcggcc agcctgcgtt cgggggggtg gctgtatctg atcgatctgg
      241 atcacaatgc cctcaatcac tacgaactgc cggcgccgat ggcgaaaatc ctgcccgaaa
      301 tttcgcgatg cctggaagag atctacgatt tcgatgccta cgcgggtcgc aagctctacg
      361 cccatctttt cgacctggga ttcgaggaca tcgaggtcag cgtgacggcc catcacgtga
      421 tctacggcca gagtcgggag gtggacagtt acgactggac caagaagatc gaggtaacgg
      481 ccccgcgtct cacggatctt ttttccatgt atcccggtgg gtacgaccgc ttcatggccg
      541 atttcaggga attcttccac aacccccggc gctttaccta cacccccctg atcctgtgca
      601 aggggagaaa gccctgagag acgggccttc aatccttgat gccgggatcc ttaaaatgaa
      661 aacgaccgct cgtgaacagc gcgatgcagg cgtccgccac atcgggatca tatgtcacgc
      721 cccggtgggt gcggatttca tcgagcgcct tttcgatgcc taacgccggc cgataggggc
      781 gatggctggc catcgcctcg acgacgtccg ccacggtgat gatgcgcgcc tcgaggatga
      841 tgtcacgccc gacgatcccc ctcgggtagc cggatccgtc catggtttcg tggtgctggt
      901 agacgatctg ggcgatgggc ggcgtgaagt cgaccttgtc caggatgttg taccccgttt
      961 ccggatgatt tttgatcagg gcgaactcgc cgctgttcag cttccccggc ttgctcagga
     1021 tctccgcggg gacattgatt ttgccgatgt cgtggatgag ccccgccatg tggatcgtcg
     1081 tcacgcgttc ttcggacagc tccatctcgc gggcgatggc cgcggccagg tcggctaccc
     1141 gccgttggtg accggccgtg tagggatcgc gggactcggc gatgagcgag atgacgctga
     1201 tcgtactcga catggccagc tggagcttct ggtagctctt tttcaactcg acctcggagc
     1261 gtctcagatc cgtcatgtcc ttgaagatgc ccaccaggct gtccacacgt ccctccccgt
     1321 cgatgttggg ggcgcagttg aaggaaaagt tgtgctcgat cccggaacag tccgggatcg
     1381 tcaacagcgc atccatcagg gtctcccggt tttccctgac gcctttcagc agctggacca
     1441 ggcgatggga atcctgctcg tcgacgaagg cggtcagggg gagtgagacg accgcgtccg
     1501 cgggatggcc caggatccgc ttccaggccg gattgacgta tagcagcttt ccgtcgttgt
     1561 ccaacgtgaa aatgatgtcc ggcgcgcttt ccgccaggat ccggaaccgc ttttcccttt
     1621 cctgcagctt ctcgtagatc cgggcgttgt tgatgctgat cccgatctgg ggggcgatcc
     1681 ccatgagcag gctgatgtcc gtctcgttga gcggccggtc ggaccggagg ttatccacgg
     1741 ccaggatgcc ctcggagcgg ccctcgtagg tgatggggac gcagatgaag gagcggctcc
     1801 ccatcttgcg ggtgaagtcc aggcttctcg gagagaggtc ctgctcgatt tcacggaggt
     1861 cattgacgag aaacggggtc tgtttgtgga acgagacgac gaaagcgccc cgggaatggg
     1921 gattggccag ggagaactcc gttttcttca ggaagtcctc atcctcggga gaatagccgt
     1981 aaccggccgt gaattccagg cgggtccggt cgccgttggc gagcatcacc atcccccggc
     2041 tgaattcaag gttcttttcc agggcggaca gcagatattt cagcagcagc tcggtgtcca
     2101 gggcgctgga gacgtactga ccgatctcct tgatcaggac ggcgttgctg tagcgggtat
     2161 tgacctggtc gagcagaagt tccgcggcat ccccgcgact ctcgaggagg tccttcaatt
     2221 cctccttttc cagcaccgag gcccgcatga aagcgcccag gatcagcagc agcgccaccg
     2281 atgagacggc gaaggcgtag cggttggcga agccgatgaa gagggccagc acgatcacca
     2341 gcgacacgag ggcggtgtag gtgccgatcc gtttccagag ggaggatcgg gtcggttccc
     2401 aggtaatgat gtaccggcag cagtctccct ccttgtgcat gcagacggga tggtcgatgt
     2461 ggggaaatct gccggtcagc agttttgaaa tgccttcgag agccccccac ctgttttcgc
     2521 actggtagag cttttcctcc acgccgggtt tcagcctgac cgtaatttcc gcctgattgc
     2581 ttttcaatgc gcgtgcctgg agcaaacacg cccggctgac ttggttatac aatttcgcaa
     2641 cgaccgaata agccgttttc gggttgatga agccggcgac atagtgggtc atggccccgg
     2701 cggctttgga aaagggggca aagcgtccca cctccaggcc gatgttcgga ttgcgcgtca
     2761 tcctgacgac catctcgtag aagcgatcga tctgcgtctg gttgaaccag tggcccccgt
     2821 cgttcagctg atagatcgtc attcccgcat aatcgagaat agtctgggca tccagatccg
     2881 gatagtgggt gcccaggtac tccatgtaca ccttgatgtt gcggctgttg tagagggctt
     2941 gatcgttcac ttcgacacca ttttcccgag aagatgcttg agcaggaacc gcagggtcaa
     3001 cctggttttg ttctccatgt attcgacgta ttccctacca tactgggtat ccagtatcca
     3061 gaggagatac cgtttttcca ccgatattcc ttcctgagag ggataggtgg cggggtagat
     3121 catgaggggt acgctgtccg tcccgtaatc caccgccaac ctgtcgaggg cggcgctcag
     3181 aacccgccag ggaaggcccg ccgggtcggt tacgacgacc ttgatgccgt tgaggagttc
     3241 ggaaagattg aatccgggag aggaacggtc gaccacgagc agggctttca gcgcctgttc
     3301 gtggagcagt tcgtagatgt gacactgccg gagaaacccg tgcctgcgat agacctcttc
     3361 caggggttcg cagctggggt ccgtctgtcc gaggcgcagc acgtccagaa gcaggccgcc
     3421 ggaggcgttc cggtagaatt gctccatttc cggcaggtgg cgggtctcga acgccccgaa
     3481 ctgccagtcc gggggcaggg acgaccgggg tccatccatc gggtgactca agtaggcgaa
     3541 aaggtccagg gagcaggccc ggggattgcc gagatctctc gcaaatccgc cgaagaagag
     3601 attggtgaag tagttttccg gcctgaaata gaacatcatg tgatcgatgc gaatcgaggg
     3661 ataccggtag aggccgtcga agaacttgag gatgttcttc aggacaaaca gccccgtccg
     3721 ctttccattc aacggtctgg ccgcaaggtg atggaccatc caggcccgct gatacgcgcg
     3781 gaaaattgaa acgtggccgt aaatccggtc attttcctgc agggtgaagt gggcttcgat
     3841 gtcctgatcc tcgcggtaca gcctacggta ggtgtccttg aaggcttcac gcgcggattg
     3901 gatgagatgg tatttctgag gatagatgaa tcccgtgtcg aagaagaact cccagagggc
     3961 gtccgtctct atctcgcaag tgaaccgggc cagggagctc cccgaatgga ccatcaggtg
     4021 gctgagatgg cggtaggcac ggaagtccat gtccaggatg gcgaagccgc agcgaattcg
     4081 tcccttcgcg attttgcgtc gatagattac ctgggcggtg caggtgatgc cgctggcccc
     4141 ggcgggccgg atttcgagat cgtggaggat cagccccggc atcaggacgg tgtcgtccgc
     4201 ctgttcctcg acggcgaatc ccgagaaggt caggtccgtg acgattcgcc gcatcggctt
     4261 tccgaggcag ggatgaacga aatgaacgga gggagggggc gtgaggcgca gacggggcgt
     4321 ccgggtcttc tgtttgcgga agcgctgaat ttccccgcgc aggggggcca gaaccatctc
     4381 ccttttccgc aagttgcccg tctggcgcag gcatcggcag gggccggaga agaggatccg
     4441 ttcgcccttc cgcaggcaga tcgtgatcga atcgtccggg ttcatccaga tgaaggaaag
     4501 gccgggctcc gcttcaaggc ggacacggaa ggcgaaggga ctgaaatcga cgagatcgcc
     4561 ttgggcacta aaaccgctct gtgtcagcgt gacctcggtg tcccggcagg cgtgccgccg
     4621 ggcgcgccgt ttgcccaaaa gatatcccgt ctccggcagg acggcggaga acccggtttc
     4681 gtcacacccc gtgagccgaa cagggaacag gatgagcgag agaccgtcct ccacgatcac
     4741 atggagcggc tgggcgtttt cggaaaaccg cggagcgcct tgagcccaga gacagcggat
     4801 ctctcccgcc gcgcaggatt ccaggcggcc ttgaacgagg agatcttcct gatgctcggg
     4861 atcacccaca tgaacgagga tattcccgtt catgaaatgg aggcggtgga tcgtgttgat
     4921 cagatgcttt ttgccgatga caggggcgga acgggtcgct tccagcaggt cccggtggag
     4981 aaagagccgt atcggcatga atgttccgtc ccccccttgc gtctgccggc acgccggatg
     5041 tccgcggcaa ggcagacgtt ttttgcccct tgattctcaa tccggcttga acaacgatgt
     5101 tgcgggaatc gccccttcat aagcctgcgc ccctcgggtc cggattgcag gctggtggat
     5161 gcttacgctg aaggagtctg tgatctcttt gggtgcggta tataccgttc aatcgtttcg
     5221 tttgtcaacg gctatctgaa aattcatgcc ttcgacagga acgcgtggat ggcgcgggcg
     5281 gcctgctttc cggcgcccat ggcgaggatg accgtggccg acccggtgac gatgtcgccc
     5341 ccggcccaga cgcccggccg cgaggtggcg ccgtcttcgc ccttgacgac gatatagccc
     5401 cgccggttcg tttccagacc cggcgtcgtg gcggggacga tggggttggc cgtggtgccg
     5461 acggcgacca cgaccgtgtc cgccgccagg acgaattccg aacccgggac cggcacgggg
     5521 ctccggcgcc ccgaggcgtc cggttctccc agttccatgc gccggcagcg cgtcccggtc
     5581 acccagccct ggtcgtcgcc gaggtattcc aggggggcgg tcaggagttc gaagcggacg
     5641 ccctcctcct tggcgtggtg gacctcctcc ttccgggccg gcatctccgc ctccgtgcgg
     5701 cggtagacga tcgtcgccgt ctcggccccc aggcgcaggg ccgtccggac ggagtccatc
     5761 gccacgttgc cgccgccgat cacggcgacc tttttccccc ggacgatggg ggtgtcatat
     5821 tcgggaaacc ggtaggcctt catcaggttc gaccgggtca ggtactcgtt ggccgagtag
     5881 atgccgctca ggttttcgcc ggggatgttc ataaagaccg gtgcgccggc cccgacggac
     5941 agatagacgg ccccgtagcc ctcggcgaaa agatcgtcca ccgtcttgag gcggccgatg
     6001 acggcgttcc gttcgatgcg gacgcccagc ttttccaggt aggccacctc cgcctcgacg
     6061 atggccttgg gaagacggaa ttccgggatg ccgtagacca ggacgccgcc cgccttgtgg
     6121 agggcctcga acaccgtcac gccgtagccc agcttgatca ggtcccccgc gatcgtcagg
     6181 cccgacgggc cgctcccgac gacggcgacg cggaggccgt tgggcgccgc gggcgccggg
     6241 ataaagacct cgcccttgtt ccgctcatgg tcggcggcga agcgctccag gcggccgatg
     6301 gcgacgggct cccccttctt ccccaggatg caggacttct cgcactggtc ctcctggggg
     6361 cagacgcggc cgcagaccgc gggcaggctg ttgtcctcct tgagcttgcg ggccgcctcc
     6421 acgaacttcc cctccgcgat cagacggatg aaggccggga tctggacctc cacggggcag
     6481 ccggcgatgc agccgggctt cttgcactgg atgcagcggc gggcttccag gacggcctcg
     6541 gcctccgtat agcccagcgg aacctccgcg aaattccgca cccgctccgc ggggggctgt
     6601 tccggcatcg gctgccgggg gatcttttcc ttcttgtcct tcctgtccgt cgtcatgtta
     6661 gttgccatgc tgtccgcacc tgtgcgctgc ataggcctcc tcctcctgcg ggcaatacat
     6721 ccgcaggcgg ctggcgagca gcttgaaatc cacctggtga ccgtcgaatt ccgggccgtc
     6781 cacgcaggcg aagcgcgtct cgccccccac ggacacccgg caggcgccgc acatgccggt
     6841 ggcgtccacc atgatcgggt ttaggctgac gatcgtcggg attgcatggg gccgggtcac
     6901 gtcgcagacg acccgcatca tgggaaccgg gccgatggcg aaaacccggt cgatcgggtc
     6961 cttccggtcg atcagcccct gtaggacctg gctgacgaac ccgtggaatc cgtagctccc
     7021 gtcgtccgtc gccacgatca ggcggtcgct cacccggccg gtttcctcct ccaggatcag
     7081 gaggtccttc gtccggctgc cgatgatgga agtcacgtga tttccagcct ccttcagggc
     7141 gcgggcgatg gggtagatga cgccgacccc cacgccgccg cccacgcaga cgacgtgtcc
     7201 gaaggtttcg atctccgtgg gattgcccag ggggccctgc acgtcgagga tcgtgtcacc
     7261 gacgccgagt cccgcgagca gggccgtcgt tttgcccacg acctggtaga tgagggtgac
     7321 ggttccggcg gccgggtccg aatccaccat cgtcagcggg atccgttccc cctgctcgtg
     7381 gaccttcagc accagaaact gtcccgcccg tcgcctccgg gcgatctccg gggccaggat
     7441 gtccatccgc acgacgtttt ccgaaagctg ccgtcgcagc gtgatcttgt tcaaaaggcc
     7501 gtctcctgaa atgcgcgaaa tttagggacg cggcgcggaa cacagctgcg tccggaggca
     7561 cccgtccccg cacgccctat agcacaggcc cggccggctt tcaaggttat ccgggagggg
     7621 atttcgcgtt ctcccgcggg aaaacgctcc gacaaacccg gaggggtgat gttcaaggcc
     7681 cccggttcgg cggcagcagg attagcggtt gtgaaatcca tcgatctttg ttagcttcat
     7741 gcccatcggc atggcgggat cgccgacact gtcgccaacg gagcaaatgc catgacttcg
     7801 acatccccat ccagcccggg caaaattccc gtccggatcg acccggacct ggccgatctc
     7861 atttccggtt ttctcgacaa gcgccgccag gacctcgccg actacctcga ccgcatcgag
     7921 atcgcctatg aacagccgga ctgaacctgc actgattcta agtgtcttcg acgattccga
     7981 tatcctgcag atcatctcaa aaaaccggtc aggaaggatg ttctcctggt ccgcgtgcgc
     8041 aggaacctga ttccatgaag cgcgtcccgt ggggcttctc caccccgacc ggcaggattt
     8101 tatgacccgc tacaagctca cgctcgaata cgacggcacc ggttaccggg gctggcagac
     8161 ccagcagaat gcccgcagcg tccaggggac cgtgcgggag gcggcccgga cccttctggg
     8221 ggcggcggtc gatctgcagg gggccggccg gacggacgcc ggcgtccatg ccctggggca
     8281 ggtggcccac ctggaagccg ccgtccgcat gccggtccgg aagcttcagg aaggattgaa
     8341 cgatctgctc ccggccggca tccatgtcct ccggaccgaa gaaacgcccc cggactttca
     8401 tgcccgccac cacgcccgtg cccggagcta tgtctatctg atctcaaggc gccggaccgc
     8461 tttcgggaag cgctatgtct ggtgggtgcg ggaccggctc gatgtgaagg ccatgcagtc
     8521 ggccgccgcg gacattgcgg gctttcacga tttcgcgtcg tttgcggacc gccgcctcga
     8581 aaaggggcac tcgacccggg tgcaggtcga gcgggcggag ctgttcgagc ggggcgaact
     8641 gatcgtcttc cgcatcgccg gatcccattt cctctggaag atggtccggc gtctggtggg
     8701 aaccctggtg gaaatcggac ggggaagccc gccgccccgt gagatgaaga ccctcctgaa
     8761 ccgcgtctcg gaagccccgg cggcatggac ggcgccccct tcgggcctgt atctggaaca
     8821 ggtgctctac gagggggatg tctggaaccc cgagccgttc gcgcccctcc ccctctgggg
     8881 gctgagcggc gcgcgtgacc gctcctgaag acggatcctt gaaaaatgat tgcacccgtc
     8941 cgggccttgt gttaaatttc gccccgtact cctctattca gcgtttccag gcaaaacgga
     9001 tccacgttgc gggtgcttga accggcgacg ggcagaaccg atgaacccga gagatgtccc
     9061 ccatccccgt ccccgcgatc gaagacgggc caggcccttt gagtgcttcc ccgttaatcg
     9121 tactggcgtt taacgtccct ggattgggga cgtctatcaa aaggaggtcg ccatgaaata
     9181 tccagatatc ccgtttgaac cgttcttcag ttgggtttgc cggccccggg tcatgtatgc
     9241 tccgggtttg cgcagcgaag tgggctacga gatggcgcag ctggggggaa agagggtggc
     9301 catttttacc gacaaggggc tggtcgatgc cggggtggcg gaccagatcg tcgaggccgt
     9361 ccggaattcg gacctggagc tggccggggt atttgacggc atcgtccagg acgcccggat
     9421 cgacatcatc aatgccgggg cgaagttcta ccggggaaag ggcgccgact gcatgatcgc
     9481 cctgggcggc ggcagcgtca tggacaccgc caaggcggtg aacatcatga tcggcggcgg
     9541 cgaggaggac ttcggccccc tggcggcgca ggtcgccctt tatgaggggg cgaagcccct
     9601 gccgccgcac atcgccttcc cgacaaccgc gggaacgggc agcgaagtca cgatcgccat
     9661 ggtcgtcttg gacgtcgagg cgaaggccaa gatctcggtc gcccatccct actgcaactg
     9721 cgacctggcc atgctggacc cggaactcat ggtcaagctg cccgcaaaga tcacggcctt
     9781 cacgggcatg gatgccctca cgcacgccgt cgagggcgcc acctcgacct ccgccgaacc
     9841 gatcagcgac gccctgtgtc tccatgccat ccgcctgatt ttcaagtatc tcccgatcgc
     9901 ggtcaaggag ccggacaacg tcgacgcccg gggctacatg ctgcttgcga gctgcatcgc
     9961 cgggatgggt ttctccaaca gcatgacggg cgccgttcat gccctggccc atgccctggg
    10021 gggacagtgc ggcattcccc acgggctcgc caacgggatt ctgctgcccc atgtcatgga
    10081 tttcaatgtt tcggagcggc ccgaacggtt catgatggtg gccgacgcca tgggcatccc
    10141 cgtggacggg atggagcccg aggccgccgg ccgcctggcc gtccaggcgg tgaaagacct
    10201 cttgaaggag gtcggcctga cgcagacgct caaggatctg ggcgttccga cggaccggga
    10261 atccctgaag cccctgatgg agttggcctc gggcgacagc cagatcccct acaacccccg
    10321 ttattgtgaa gaggatgata tcctggacct gtatatgaaa gccctttaaa tctaaaaaat
    10381 ggaggaaaga aaatggaatt ctggaaagac gaatcggaac cgatcaaggc gttcctggcg
    10441 ctcttcgatg cgtcggcgaa agaccccgaa ctgtccgaag gcctgaagaa ggtcaaccag
    10501 ctcatctggt tcgactacac ggagagcggc cccaactgct ccttctgggt ggatacacgc
    10561 ggcggggcgc tgaagaacgg cgccggcaag cccgacggcg aaccggacct gatcatgagc
    10621 ctgtcggccg acgacgccca ccggtcctgg tccgacaagc tcaacccggt catggccatc
    10681 acgcgcaaga agatccgggt caagggttcg gcgacggggc tcctcaagct cgcccccaag
    10741 ctgaagaagg tcgccgcgat ttacatgacg gtcctgaagg acctgggctg ggaagacaag
    10801 atcatcaaat aacggtcagg ggcgttcggc atgggctaaa ggccgaaggc gaagggccgg
    10861 ggggagaggg cggtttccgg cccttcgccg gggcaccgtg ggcgttgcgg tccgggacgg
    10921 cagatcgggg ctcgggcgcg tcgcgacggg ccttcggccc cggcgccacg gggatgttca
    10981 gaggaagctg gacggatgcc tcggcgtccg gagaaggatg aggggtggcc tatgaatggc
    11041 aatacgggca agattgtcag cgtggacctc acggccggtc aggcgacggt ggagaccctg
    11101 ccgggggaag actaccggaa atacatcggt ggcagcggac tggcggcgaa gctcttctgg
    11161 gagcgcggaa acttcgctgc cgaccccctg tccccggagg cgatgctcct gttcatgaac
    11221 gggccgttcg cggggctgcg cctgtccggg gcgagccgga acagcgtcgc gggctgttcg
    11281 ccgctgacgg gccactgggg ggactcctcc tgcggcgggt attttgcgcc ggagctgcgc
    11341 tatgccggct tcgacggcct gatcctcacc gggaaggcgg ctcggccgtc cctgctcctg
    11401 atcgtggacg acaaggtcac catcgaggac gccggggcgt actgggggaa ggggacggag
    11461 gaggtcacgc aggcgctgaa agcgaagtac gggaaggact accggaccct ggtcgtcgga
    11521 ccggcggcgg agtgcggcgt ccgctacacg atgatcctga acgacgggca ccacgcgacc
    11581 ggccgggccg gcttcggcgc cgtcatgggg gcgaagaacc tgaaggccat cgtcgtcaag
    11641 gcgacgaaga aggagatgac gctggccgat ccggaggcct tcgaggccct tcgcaaggac
    11701 ctgaacgtcc ggatcaagca ggccctggcc tcggaggtcc tgcacgaaaa cgggacggcg
    11761 gcgaacctga tgggcggggt gtacagcggc gacgttccgg tgaagaactg gacgtccaac
    11821 ttctgggaag aggcggccga tgcgctcacc ggcagcaccc taacggacac ttatctcacg
    11881 cggcgcggcg catgcgccta ctgcggcatc gcctgcaagc gctacgtgga ggtcaaggac
    11941 gggccgttcg ccgttcccga cgggccgggt ccggagtacg agacgatcgt ggccttcggg
    12001 acgctgatcg ggtcgatcga tctggccgcg gcgtgcaagg ccggccgggt ctgcaacgac
    12061 ctcgggatgg attccatctc ggcgggcggc acgatcgcgt gggcgatgga ggccttcgag
    12121 cggggggacc tgacgctgga ggatacgggc gggatcgccc tgaactgggg cgacatggac
    12181 acggtggtca accggattct gccgatgatc gcccggcgcg aggggaagct gggagacctg
    12241 ctcgcggacg gcagcgtcgc ggcggcgaag aagatcggta ggggcctgga ctatacggtc
    12301 cacagcaagg gactggaggc gccgatgcac gatccccggg gcgggggcca cgggatggcc
    12361 ctgacctacg cggtcagtcc gcgcggggcg tgccacgtgg cggacccgat gctctttgtg
    12421 gagatgggcg cccgctacta tcccgagatc ggcttcgatt ttatcctgga gccgaaaacc
    12481 agcgagaaca aggccgaggc ggcggtgacg tcggtcgccc tgggggcgat cgaaaacagc
    12541 gcctgcttct gccagtttgc cgacgcggag gtgacgattc ccgactggct tgccctgttc
    12601 aacacggtgg cgggctataa ctgggaggcg gaagatatgc tggcggccgg gcgacgggtg
    12661 tttttcctca agcgcctgat caattaccgc tacggcctga cggcggcgga cgacgccctg
    12721 acgccccgca tgctggaacc ggggaaggac ggcgaggcgg aagggtccca ggtggatctc
    12781 gaggccatga agtcgcgttt ctacgaactg atggacatcg accccgtccg gggcatcccg
    12841 acgccggcgg cgctggcggc gctggacatg tccgcggagg cgcaggccgt cgcgtagcgc
    12901 gacccgatgc cgccggccat cgctgtgtca aagcgcaccg gaggggtggg atcggcctga
    12961 aaacacttcg cgacaaaaaa gcccgttcga gggaacgggc ttttttgtcg cgaagtgggg
    13021 cgaccccggg ttacggcacg acgaatttga agttgtgaca ctgggcgcag aaattttcgg
    13081 acttcgcatg ctgatgatgg cagaggtcgc aggcggtata ggtgttgccg tggggcgatg
    13141 agtgggggtt ctggggcttg acgttggccg tcctggccgc caccgtctcg aagctgccgt
    13201 ggcaggccag gcactgctcg gtggagagct cctggggtgc gttcaggtcc gtgtggcatg
    13261 cttggcaggt gatgcccttg agctgatgga agtggctcag gggcattttc gtgtagagcg
    13321 cgagcggact ccccttctgg tggcagccca ggcaggcctc gccgttcatc gcggcggtcg
    13381 gggcgtggcc gtccggcagg accttgtccg cgccgtgaca ggcggcacag tctcccgcct
    13441 tggccgttgc cagcttcttg ggtgccgcga ggagcgatcc gccggacgtt gcggcgaaca
    13501 cgagaaccag ggccatcagg aagaaaattc tcagcgccgt ctgccgatcc gtctgtttgt
    13561 ccgttttcat cttgttcctc ccgagtggtg atggtccgtc cggaggggag agccgcggcc
    13621 ccgaggtgca ccgcccctcc ccaaccggtt ccttttagcc ccaggccttt tccctgaccg
    13681 cgttccgtcc ggcgatacgc ccgaacgtca gacagtccag tacggcgcaa cttccgagac
    13741 ggacggcgcc gtggacgcct cccgtcacct caccggcgca gtagagaccg gggatgggct
    13801 tgtcatcctc cacattgagg gcctgcgcct ccacgttgat gttgacgccg cccatcgtgt
    13861 ggtgcacctt gggcatcagc cgtgaggcgt agtagggggc ggtgtcgagg gctcgctggt
    13921 tcttctggag gtaccggccc cattcctcgt ccttgccggc cgctaccgcc ttgttgaagt
    13981 tggcgatggt ttccctcagg ggggcgatcg ggcagtcgta ggcctccgcc atcgccgcca
    14041 gcgaatcgta gcgcttaacg acgccccgtt ccatgagctt ggccatgatg tccccgagca
    14101 tcttctggcc gatggcgaag ccgttggcgt cggtgaaggc gatgcacttg ttgccgaccc
    14161 ggataatggc gtcagcacgg atcttccggt cggccagttc gttgacgaaa cgcttgccgg
    14221 tctgggtgtc gacccagagc ccgtacatgg cacagcacaa ctgggcgaag aagggattca
    14281 gccccatccc cttttcgtcc gggctgcccc aggggccgag ctggatccag gagagctgca
    14341 ccggggtgca cccgatccgg aaggcctcgc gcagcgcctc tcccgtggcc ccgggttggt
    14401 tcgtggattc gagatcggcc gtcaaccggg gatcctgaat ggtccggaat gggacgtcgt
    14461 tgccgaagcc gcccgtggcc aggacgaccg ctttcttcgc cttgatgaac ttcgtcctgc
    14521 cgctgttttc tctcgggaag acatacttct cgcggacctc gacacccttg acccgcccat
    14581 cggtatcccg cagaaggcgt ttgaggagga cctgggtgcg gggaataaga ccgatttcct
    14641 tcagcttttc caattgctgc gtcacgatgg ccgaaccgct ctggttgtag gtgctgtaca
    14701 tacgcgggac cgaatggccg ccctcctgcg acagggaatc cttgtacttg acgccgagtt
    14761 cgttgatggt ccactggacg gtgttgttgg attcgtacgc caccagccgg gccagctcaa
    14821 ggtggttcag gttgagaccc gctgcaagca tgtccttgag cagcagttcc ggcgaatcct
    14881 tgatgccgtc ccgggcctcc aggggtgagc cggcggcgga gacgacgccg ccgttgatga
    14941 tggaattgcc tccgggggtc cgcatctttt ccagaaccac gaccgaggcg ccggccttct
    15001 tggcttcgta ggcggcggca agccccgcaa aaccgctgcc gacgaccacg acgtcgaacg
    15061 tttcgttcca tttcttcggc atcgcggcgg cttccgcctc gcccggccgc caagccatcc
    15121 cggtgaccgc gatccccgcc gcggtgcctc ccgtgacctt caggaagtca cgccttgaaa
    15181 ccgctttttc cttttctttc atcagggaca cctccattcg aaaaaacgtt tttcaaacag
    15241 gttgacttga ggccgcatcc cccttttcga ctctcctatg gcataaaatg ggagaaaaga
    15301 aaacgagaat tgcgccggcg tcttcccgcc ccgcggctca aacacccctg tcgcttcagc
    15361 aggacgaaaa aagcccccgg cagcgatcct gccgggggcc gagcgagacg agacgggcgg
    15421 tgaatcgacg gaccccgcct atttctgctc cttcctcacc caagcctccc gatagaggcg
    15481 gccgatcgtc gtcacgatgg cgaggaagaa caggccgagc atgaagttgg gggccgtgcc
    15541 gagccactgg tccaggcgat agcccaggta gaggaagagg aacgaggcga tcacgatgac
    15601 gaatccccag gcgctgatgg tcatgatacc gtgcaggcta ttgaggttcg ttccggtctt
    15661 ttgcgtggtg gcggtcatgt ttcgttcctc ctttccaacg gtttccgcgt ccggtcgggg
    15721 acggttggct agctcaccag ggtcttcacc acgttgacga cgggggaggt gaagagggcg
    15781 acgaggacgg tgtagagggc gaaggcgccg acggcctctc ccaggtagat cttttcatac
    15841 ttgtgcttga gggaaacgat gatgagcccg ccgacgatga ggcagccgag ctggaagtag
    15901 gggaaggcgt ctcccgcccc gacggcgtat tcggcgaagc tgaaggtggc cgtgaggagt
    15961 acgactgcga tcgtcacgat gagggtggaa acggtctttt tcatggtcta tgtcctcctt
    16021 gaagggtttc tttcgttttg cctatttata tgagaaaagc gtgccacagc gacgggcacc
    16081 ggcaataatt catgcaacta tttgatttat tgttgcaaag atgaattcgg gcatttcggt
    16141 cggcggtccg tttgacccct ctgcaggcgt ccgcttggat gaaacgttca acggcgttga
    16201 aaggaatgga gaggcgccgg gagagcgccg ggtgtagctg aaacgcgttc ccacccaggt
    16261 cggccgtcgg cggttaatcg cataagcgcc atccaatcaa ttcgaatttc ctaagtaaat
    16321 cgatcgtctc gacggccaaa caggtgtcct ccagggtggc gatgccggct tgggtggatc
    16381 gcgaaaagcc gccgctcagt gtctggcagg ccgacaggaa ttgaaggcag gcatccgcat
    16441 ggggaggcgg cctgtttaaa taggctgaag ccaccacgcc cgcatggata tgctccagga
    16501 aggagaaggt ggtgaagggg atatcgacaa acccgcagac cggatcggcg cattgcgaaa
    16561 caaaggcctc gcttcccaag atctgcaggg gataacccag cctatcgagg attcgcaaag
    16621 cctgggcggt ttccaaaagc gttgcgtaga atgcgccgaa gcctccgtct tcttttcgaa
    16681 aatgcatgac aaatcggatc acggtctcgc ggagggaggg cgcgagcgca atatgccggg
    16741 cggtgcagag ctccacgagc atcagcatcc gccggaagat ggacgtgact tcggccggca
    16801 atttctcgat ctcgtaaaca tggagatgat ccaggatgta ggatgtcgga tcggcttccg
    16861 gctcctcccc caggagtttc agcgattgca gacagtagaa cgcctggaag atgctctcat
    16921 aggcgccgtc ctgttgttgg cgggccttga gaaaccccag ggtgcgatcc tcccggaacg
    16981 ggacgccgag caggtggagg atcgagagag cgtagtacgt gtcggagccg ttgggttctt
    17041 ccagacgata aaaacagaaa ccgccctccc ggcaacggcg ttcgaggaca taacggacaa
    17101 tccgatccac gggaagacta tgtccagcca cgattccgtt ccctcgatac ggtgtctttc
    17161 atgcccaaag gataacagcc agaggcctcc gaaagcattc gtacatcggc atccgtccat
    17221 ggcagcttgt atcttcggcg tgcatcgttg aaaaatgcga tggtctcctc gatcggcagg
    17281 cgtttaacga cgacatcgat cgccggcagc aattctgcgc ggaggcggaa cagatcacgc
    17341 gcggggagga acgtttcagc gatggccaca aacaaatagc gtttctccag gatcagataa
    17401 cgaagcaggg aagccttggc ggctgtcaat cgggcgaggt tgtccaaaac gatgatgtgc
    17461 tgacgggaat ccttgagggc gatattggcc gtcaggtaac gtaacgactt ataggtcctt
    17521 gcttcaatct cgccggttga ccttccggcc gaaataaatt ccttgagcag gcgtcggcaa
    17581 acctgggtgg gcgcgagtga aaaatcaacg aaaacgaacc tccagttctt ttccgtgaga
    17641 gcggcaatgt gcttgatcaa gatcgtcttc ccgatgccgt gaattccggt taggacgaca
    17701 ttttgacccc gttcgagagc ccggataatc cgagcggtat ccttttcgcg accgacaaac
    17761 atgccatcac ctgatcagga gtcatcaacc gcatgggcag gggcggactc catcgccggc
    17821 tcaggctatt ttgtggaacc ttttttcatg ggattctcga aacaggcgtc cgcacccaac
    17881 tcggcctcga tctccagcag gcggttgtat ttggcgattc gttcgctccg gcaggctgag
    17941 cccgtcttga tttgcccccc tcccatggcc acggcgaagt cggccaggaa ggtgtcttcc
    18001 gtttcccccg agcgatggga aatcacgatt ccccatccgg ctttgcggca taaatcgacc
    18061 gcttcgattg tttccgtcac cgtgccgatc tgattgagtt tgatgaggac ggcattggcg
    18121 gttttttctt cgatgccgcg tttgatgaaa cgggagttcg tcacaaagat gtcgtcgccg
    18181 acgatctgga tccgggtgcc aagcaggcgc gtttgcgcct ggaacccctc ccagtcgttt
    18241 tccgccaagc catcttcaag ggagacgagc gggtattttt gcaaccagga atcataaagt
    18301 cggttcattg cggcggcgtc tttctcgccc tgcccggatt tgacaagccg gtaacgcccg
    18361 ccttcgtaaa aggaactcgc ggccgggtcc aaagcgaggg ccacatcccg gccgggttgg
    18421 taaccggctt tttcaatggc ttccagaatc acctcacagg cctcttcgtt gctcttcaaa
    18481 ttcggcgcga agcccccttc atcgccgacg ccggtcacat gtcccttctt gctcaagata
    18541 cccttcaagg catggaaggt ttccacccca taccgcaggg cctcggcgaa ggtgggcgcg
    18601 cccaggggca tcaccatgaa ttcctgaaaa tcgatgctgt tgttggcgtg cttgccgccg
    18661 ttaaggatat tcatcatggg tacgggaaga cggatcttcc gggatccggc gagataggta
    18721 tagagaggcg tggacaggct cagggaagcc gccctcgcca cggccattga gacgctgagc
    18781 atgacgttgg ctccgaggcg gcccttgttc ggctggccgt cgagatcgat catcaggcgg
    18841 tcaatttgtt cctgcgcaag tggattcatg ccaatcaggg cgggtgcaat tgttttgttg
    18901 acgttgtgaa cggcctgcag cgttcccttg ccgccgtacc gctttttatc accgtcgcgc
    18961 agctccacgg cctcgtgctc ccccgtggac gccccggagg ggcaggaggc ggaggcggaa
    19021 atcccgtcat ccaaggtgac aaaggcgcgc agggttggat tgcctcgcga atcgagtatc
    19081 tccatggcgt gaattctctt tatttttgga ctcatgacgg tgttcctttc cctgtaagga
    19141 tatcggaata aatttctact tcaccttttc gaacagattc actatttctt gaatgttctt
    19201 ctcgatgtcc aatgtctgtc cttgttgccg ggagaggagc gcctcgatcg tcgtcagctc
    19261 gtcccggatg tagcgctcgg cctgcttccg catctcttcc atggccgcgt tgatccgggt
    19321 ttcccactgg aaggccaggc gggagagatt gacctccacc tcacggtcga tcatcttgag
    19381 gaagtggcgc tcaaacaggc cgcggaagag ggtcatgggg atcaggaacc acagcaggtc
    19441 gaagtggaaa tcgaaggtct tggccatgtg gatgtcgggg tgttcaggct cggcgacctc
    19501 gatcctccag tccgcctcgg cgagccggac gcccaaaacc cgctcgatgt tgtcgctgag
    19561 cagcgcccgg aaggaggcca gcgaacgatc cagactggcg tgagccttct tcagcgttcc
    19621 catgaaatgg gcttgctccg tccgcgagat ccgcccgatc tcctcggtga agttttccgt
    19681 gagccagtcc tcgtaccgcc gggtgagttt ccacaggttg cccgtccatg ttgccatgtc
    19741 gcgtttgagt tgcgtggcga gtttctcccg gagcgccgcc gccagggggt tcaggttccc
    19801 catgatgaag gtgcgcgtct gccgcgaatt ctcccgggtg atgacggtca gctcttcctg
    19861 gagaagggtg tagttcaccc gctcgtccaa aatccgctgc cgcaggccct tccgatcttg
    19921 atcggcctgc tgggacatct tccgggcgat ctccaggtaa cccaggcatc cccgagccag
    19981 ggaccgggcc ttgtgcttga ggatgtttct gaattcgcga tcgcggttgg cggcaagggg
    20041 cagcaggacc tccatttcca ggcgcgtttt gaaccgctcc gtctcctggc gagtggagta
    20101 gagcagaacg ggaaacttcc ggttcagctc gcgctgtagg gtgtcctgga agaaggtgac
    20161 gacctcttcc tgctggtcgg ggtcaagcag gtccgacttg gtgaggagca ggacgatccg
    20221 gggggtgtgc gccgtcaggt cgcggatcag gtgcaggtct tgttcggaaa gggggcgatc
    20281 cgcgctgatg gcgagcaggg cggcgccgac ctcgggcagc cagttttccg actcgcggat
    20341 gtgcgcctga aaaacgctgc ccagcccggg ggtgtcgacg aggcacaacc cctcgtagtc
    20401 ttgcaggccg gaaagctcga tatcgacata ggcgatgttc ttttcgttgg acggattttt
    20461 ggcctccgcc gtgaagtccg ccagggaatc gaggccgatc tcgcgatttg cgccgtcgaa
    20521 aaaacggacc cgggctaaat ccttcggtcc gtactgcatg cgggtgatca ccgtggtgac
    20581 ggggacaacc ccgacgggca ggagcgtccg tccgatcagg ctgtttaaga aagaactctt
    20641 gccggccttg aattgtccga ggaccgcaac ggcgatgggt gaattttgcc ggagaagcgc
    20701 ttccgccgcg tccagcggac gccgcaggga aatcatctgg aagcgttcga cggcgtcgcg
    20761 catgcgcgcc atgagttgcc cgcacgcatc cgacaagagg tcgggcatgg atgaatccgg
    20821 tgcggatgtc ataaaaaaac tcccccgggt ctgcggcagg agtcggatcg gctgtgggcc
    20881 cctgccagcg ttgtggtagg agttatcagc ctggaggggc agtttattcg gtgaactcca
    20941 tcaccgaggg gacctattaa ccgaccgcgc cctgtttgtc aagcgaaagc gggcctgacg
    21001 gaaaatcgcg gcgatgtttc acgatgttcc ccttgcgcgc ctgacggggc cggggccgat
    21061 ttccgggaca aacttgacaa gcgaccggga ttgttttatc tttccgcacc gtgccggggc
    21121 agagtccggc gatttctcct gaaaggcagg tcccgcgtga ccgatccgaa gtctcccctc
    21181 cgctccttct tctcctccgt gaggctggcc ttgttcctct tgattgcgat cgcactcacg
    21241 gcgatcgccg gcaccgtgat tccccagggg agcgccgccc cggagttcgc cgcccgcctc
    21301 accccgagca tggccgccct cctgcagaag cttcagcttt tcgatgtctt ccattccctc
    21361 tggtttctga ttctgatcct tctgctgacc gtcaacctgg tcgtctgctc gtggagccgc
    21421 tttcccgctt ccctggtccg ttggcggcag gcccgctcgc ccgcaccggc gccggagggc
    21481 gccttcgacc ggctttcctc cggaaaccgg atccgtgtca ccggggaggt cccggcggtt
    21541 gcggcgatgg tggagacacg gctgcgcaag cgcgtcggac cggtccggac cgctccaggg
    21601 cgggatgcga ccatcctctc cgcacagaag ggcgccgcgt cccatttcgg cgtgtatctc
    21661 gtccacctgg gcgtcctcgt tgtcatggcc ggggtcgtcc tcggattctt tctgggattc
    21721 gaagggtccc tccaactggg cgaaggggag gcggcggatg ccgtgaggac gaaggcaggg
    21781 ggcgtccaga ggctggattt caccgtccgc tgcgaccggt tcgtcctgga gcactacacg
    21841 ggcgggatgc cgaagaccta ccgctccgat ctgacgttcc tgaagggggg gaaggtcctc
    21901 cgccggacgc cggttctcgt gaatcacccg gtgaccttcg gcggctaccg gttctaccag
    21961 gcgagctacg gcacgatccc gggcggtggc gcgaccctgg ccctccgccg cgaaggacgg
    22021 gcgctgggat ccgtcgaggt tggcgtgggc gccggcttta acctccccgg cggggaggga
    22081 agggtggagg tccagcggat cgaggagaac ctgatgcaca tggggccggc ggtcaagctc
    22141 ctgatccgga cgccgggcga ggagaggccc ctctgggttt tccagcacct ggagacgatt
    22201 ctaaaggaac agccgaacct gtttcagatg atgcccatgc tgaaccctgc cgggtttacg
    22261 ccgtacgaat tcgcccttgc gcggctctct ccccgctact acacgggtct gcaggtcgcc
    22321 cgcgatcccg gggcgccggt cgtggcggcg ggggcggtgc tcctcgttgt cggtttcctc
    22381 ttcgttttct tctacgccca ccgccaggtc tgggtgcgcg tggcggcgga gaacggggcc
    22441 gtcgagatcg ccgtcaccgg aaggacgaac cgtgaccggg tcggcctcga gcgggagatc
    22501 gcgtccctgc tcaaggggtt ccgtgaaggg ggcgccggat gattaacacc gagatcctga
    22561 gctgggtgac cttcatctat cttggggcct ttctgctcta cctgctgcgc atggtgaacg
    22621 gccgggaatt ctggggcgtc ggggcttcgg gcgcggtggt cttggggtgg atcgcccata
    22681 cggcgggcct gctgatccgc tggcgcgagt cccatgcgct gggcatcggc cacgcgccca
    22741 tgtccaactt ttatgagtcg ctcatctttt tcgcctgggc aacggcgctc ttctacctga
    22801 ttgtcgagtg gcggacgcgc aaccgcagtc tcgggacgtt cgtcctgccc attgtcttcc
    22861 tcctgatggc gttcgcgtcg ctgtctcccg gcatgaacga ccgaatccag ccgctcatcc
    22921 cggccctgca gagcaactgg ctgaccgtcc acgtcttcac ctgcttcctg ggctatgccg
    22981 cctttaccgc ggccttcgga ttggggatca tgttcttcct caagcgcctc gaccgccccg
    23041 cccgctttgt cggcctgatt ccggcggcgg aaaccctgga cgagctgctg taccagagcg
    23101 tcgtcctggg attcgtcctg cttgctctgg ggatcatgac gggctccgtt tgggcccatt
    23161 acgcctgggg gtcctactgg agctgggacc ccaaggagac ctggtccctc atcacgtggc
    23221 tcatctatgc cgccctgctt catgcccgct atgtccgggg ctggcgcggc aaccggatcg
    23281 cacttctttc cgtcgtcggt tttgccgcca tgctgttcac ctacctcggc gtcaactacc
    23341 tgcccagcct gcacagctac ctgtaaacgg tcagaagaag gtccgccccc ggaggggcat
    23401 cctccgcttg ccaaaatccg ggaaatgcgc taaaagcgcc cacgtcgcgg gcggcggcga
    23461 gatgcgcccg ggtgtgaaag aaatgaatga aaggggatcg tgaagcagtg tgcggcctgc
    23521 cccgcgacat gatcgttgca tgaaaaaatc gcggttattg ctcatcctcg ttttggtttt
    23581 ttgctgtctc ccgttccctt ccgccttgca ggccgtcgac ctgcaactga accgcacgcc
    23641 ggtgaaggtg tttttcagcc cctccggcgg ttgcacggac gccgttgtga cggagatcga
    23701 ccaggcccgt tcggaggtcc tcgtgcaggc ctacgtcttc acctccgcgc cgatcgcgaa
    23761 ggccctgctc caggcgcacc gccgcggtgt ccgggtcgag gccatcctgg acaagagcca
    23821 gcagacccgg cggtattcat cagccacgtt tctggtcaac gccggcagcc ccacctacat
    23881 cgacggccag cacgccatcg cgcacaacaa ggtcatgatc atcgatggcg agcgcgtgat
    23941 ttccggatcg ttcaacttca cgaaggcggc ggaagagagc aacgccgaaa acctcgtcat
    24001 tatccgttcc gccgaactgg cccgggtcca cctggcaaac tggcgggagc accgtgccca
    24061 ttccacggcg atcgatcgcc tgacgttggt ccaaccatcc gcaggccgtc cgcaggaacc
    24121 ctgagcggat ttttccattc gaaatccccc ccgccgtcgt tcgccacgaa caaggcggcg
    24181 aggcccctgc cggggcggga ggcaagaggc cggaggttga accggggcga agcgcggccc
    24241 ggtcttgaga cgcccccaaa aagaaaaaag cctcggaagc gaagggtttc gtcggctttc
    24301 ttctttcaag aggaaacggg atgcgcggct tccggtcccg gcgggacgaa tccgtccggc
    24361 ggacggctca gcgccgggtg agtcccatga tctgccgggc ctcgtccggg gtggccggct
    24421 ccaggcccag gccatcgatg atccggacgg cggcctccac cagttcgtag ttccctttcg
    24481 ccaactcgcc gttgggcatc cggatattgt cctcgagccc cacccgaacg tgtcccccgc
    24541 acaggatcga ctgggtgatg acgggccatt cctcgtttcc cacgccgcag gccgtgaagt
    24601 tcgcgcccgc cggcagggcc tggaccatgc tcgtgaacat ggcgggccgg aaggactgac
    24661 cgccgcagac gccccagacg aaattgaaat tcaagggcgc ggtgaagtgg cccgatcccg
    24721 aaatggcgta gaagttgtcg atgtgggaag aggtgtagca ctccatctcc ggcttgacgc
    24781 cgttttcctt catctgccgg acgaagtcgc tgatgacgat caggctgttc tcgaaggtgt
    24841 agtccacgac gacctcgccg gttttccggt taaccgccgc gaagttcatg gtccccatgt
    24901 tgagagaggc catctccggt ttgacggcga caatctggcc gatgcgctgt tcgggcgtgg
    24961 cgccgggtcc cagggccgag gtcaggttga cgatcaggtc gggacatttc tgcttgatgg
    25021 cgtcgtgacc ctcgcggatc cgctcgaccc gcgtcgtgtt tgtaccgtca tcctcgatgc
    25081 cgtggacgtg gaccatggcg gccccggcct tgtgggcgag cgccgccgcc tcggccagtt
    25141 ccttgggcga gtaggggacg ttggggttct ggctcttccg ggtcgaggag ccgaccaggg
    25201 cgcaggtgac gatgactttg cgtgacatga ggcaatctcc ttgtcgttcg atgggatgtc
    25261 ggttggggcc ggggcgggac gaaggaggga tggcgtccac tgccccggtc ccacccgtta
    25321 gagcttttcg ttgacgatct tccggatctc ccgcttcagg atcttgccga gtccggtctt
    25381 cggcagggcg tcaaagatga agatcttctt cggggccacg aagtggtgca gatgctggtt
    25441 caggtaggcc aggagttcct cctcggtggc cttctgtccg ggtttgaggg tgacgaccgc
    25501 ggcgacctcc tcgccgtact tttcgtccgg gatggcgatg acggcgcact cccagacagc
    25561 gggatgcttg tagagccagc cttcggcgac gcccggatcg atgttctccc cgcccttgat
    25621 gatgaggtcc ttcacgcggc ccgtgatgta gagttccccg tcgtcgtcca ttttacccag
    25681 gtcacctgtg tgcagccagc cgtcgatgag ggccagcgac gtttctttgg gtttgttgag
    25741 atagcccttc atgatgatgg ggcccttgca gatgacctcg ccctcttcac cgcggggaac
    25801 ttccttgccg ttcagatcga cgagcttcag ggccaggccg ggcagaggga agccaacgct
    25861 gccgggcttg cgcggccagt cgcgacgctg ccgggcgatg gatccgccga cctcggtgca
    25921 gccgtagccc tgggtgatct ccttgccgaa catcttctgg gccttctcca gggtttcgag
    25981 cgcgagcgga ccgccggagc agatgaggag gtcgagcgag gagaggtcgt ccttggccgg
    26041 atcgtagacc atcatgaggt agatgagcat cgtggggacc acggggatga agctcacctt
    26101 gtagtcccgg atggcgtcga ggaccttgcg ggggtcgaag ggaggcatca tcaggctgac
    26161 gcagcccagg gagtacagct caaggcagct gatgacgccg tagatgtggt tcatcggcac
    26221 cgttgtcagc atgatctggc ccttcttccc gccccagacg gactgtcccg tcctcatctg
    26281 ggattccatg ttgcggtgag agagcatgac gcccttcgga aagcccgtcg tcccggacgt
    26341 gtacatcagg atggccatgt cgtcggggtc caccggtacg acgtcccccc gattgtcgat
    26401 ttccgtcagc aggctggcgt agggggtgat gtccgcgccg gtttcctttc cgatgacgat
    26461 gatcttctcg atcgtggcga cgccctgggt ggcttcccgg accttggcga gcaagctgtc
    26521 ttccgtgatg accaccttgc atttggagtc ttcgagaatg tagcggattt ccagggccgt
    26581 caacatgaat atgatcggta gataaacgcc gccgccgcgg ttgatgccgt tgatgatctc
    26641 catgacctcg gggatattgc cgacgacggc gcccacgatg tcgccctttt tcacaccgat
    26701 cttccgcagt cccgtggaca gggcgcgtgc ccggcgccgg atctccgtgt tcgtgaaccg
    26761 tttctcgcct tcggggccga gatagatgaa ctggtcgtgt tctccgaaga tcgtcacgtt
    26821 gtcatccaga aaccggctga tgttgttctc cacgctgcac ctcctggggt tttgatgtac
    26881 gttaaaagtg accctgacgg ttggggccgc aggatgcttc ggacgctctg acgggggttt
    26941 tctatcagag cctgactgac cagtcaatct atttttctcg tctgggcgaa aggcgccccg
    27001 tcagcgcaac cataccgcgc ggacgagtcg ggggagagct tccgtgcgat cgtttcaggg
    27061 gctatttcgt gattcgtctc aggaggaaaa aggcgaagat cagaagcccg accacgataa
    27121 ccaggaataa gacgccgtat tgatacaatt gctcaaggcc atcgtaccag tccatgatcc
    27181 gttccacata cgtctgatag agttcctcga tttttcccat tgcctgatcc tccggataga
    27241 agggttgcgg tcggcgcgac cctaggcttc cttaccgtgt atcatattgt gtacaggacg
    27301 acaaggcagc gggtctccac gttggagagg cttttgagct tgtggggggt gtcggagttg
    27361 aaatggatcg actccccggg atggaggtga tgggccttgc tgcccagggt gagctccaac
    27421 tcgcccgcca tcacgaaaat gaactcctcg ccctcgtgct tgtaggccac gggcttgtgc
    27481 gcctgtttcg gatcgatggt gatgagaaag gccttcagat ggtcgtgcgc cgcctccggg
    27541 gtcagattct gatacgagta gttctgggtg cgcttgatat aggcctgggc ccgctggtcc
    27601 cggaggtgcg tcttttcctc ccggctgagg aaggtcgagg gatccacgcc cagggccgcc
    27661 gccaggcgca gtaggaaggc caccggcggc gcgacctcgt cgcgctcgac ccgggcgatg
    27721 aactccgggg tctgcccggt ggcctcggcc agtgcggcgg gcgtccagtc gcgggcttcg
    27781 cggattttcc tgatccggtc cccgaaggat tccgctccgt ccttctcgtt ctcgtcccag
    27841 atcagatcgg ccaggccgcg catgacggtc tgcttaacct tcgccgtgag gaggggcaga
    27901 aaggtcctgg catcggccac gacgccgata tccgcgaggg cgaagatcgg ggcctcagcg
    27961 tcccggttga cggcgacgat cgtttcggat tccgcgatgc cggccgtgta ctggatcgcg
    28021 cccgaagttc cgactgacac gagcaggcgg gggcggaccg tcttgcccgt ttggccgatg
    28081 aggcggtctt cgtccaccca gtgctgggcc acaggaggcc gggttgctcc gacctccgcg
    28141 ccgagggcga cggcgagttc cctcagcagg tgaaacccgt ccgcgctgcc caggccgccc
    28201 ccgccgacga cgacggtctc cgcgtcctcc aggcaccgtc ggccttccgg ttcggtttca
    28261 cgggacagga gcgtcagccc cggcggcgga gtcaggctgt cgatcggaag gcgctcggca
    28321 acgccgggtg tcccggcgaa ggcgacggcc gcgcggatgt ggggctggac cgtggcgaag
    28381 ccggtccgat ccggatcggc ataggtgatt tcggcggtga tttcccctcc ccaggacggg
    28441 caggaggcga cgatgccgcc ggcctgccag cggaggtccg cacagtcggc gatcatgccc
    28501 gcatcgtgca aacgggccgt gcgtgcggca agttcgcgtc cgaagtccgt cagcgcacac
    28561 aggacgaggc gcggggcgcg ttgacggacg gcggacgcaa gggctagggc gaaacaatcc
    28621 gcccgcggcg cggccaggag ggggtccgcg atccggtaga cgaggtccgc cccgtgcgcc
    28681 agaccgcgcg cgtccgcctc gtccggggtg agtccccccg gctccgcggg cgccggagac
    28741 ggggactcga gaacgacgac cgccgcccgg cccgagaccg cttgggcgag ttcccgcgcc
    28801 ttggccagaa ggttcagggt gtagccgaag agccgctcgc tcctcagatc ggccaccacc
    28861 cagatttccg tgtgggtttg catgggcatt gttctatccg atcaatcccc ggtcggcgag
    28921 tgtccggacg agggcctccg cgacctcctc gctggcgccg gtcaggagtt cgcatttccg
    28981 gtcccgggtt ttcttcgcga gggagacaac ccgcgtgggc gagccggctt cgccgacctc
    29041 ggcgggatcg cacccgaggg tgtcgagtcc ccaggtctcg atgacgccgt gctcgtaggc
    29101 ggcatcgatg ccgcccaggg tttcgtcccg gggcgtgacc gcggccggat ggatggtcat
    29161 ggcggcgggc agcgtcaccg caaaccgttc ccgaaagccg tccgcccgtc gctccgcgac
    29221 gaatcccgac gccgtccgct ccagggcatg cacccacgtc acgagcggta acccgagccg
    29281 gacggcggtc tggggaccga cctggcccgt gtcgctgtcg gaggtccggg tgccaaagag
    29341 gagaaggtcg tacggcgcga gacgggcgac ggccgcggca agcgcggtcg aggtggccag
    29401 ggtatccgcg ccggccaagg cccggtcgca gagcaggacg gcccggtccg cccccagggc
    29461 cagggcctgg cggagggcgt cggcggcggc gggcggcccc atggacaggg ccgtgatgcg
    29521 tccgccggtc tcttcccgca ggcgcagggc cgtctcgagg acgggccggt cgaaggggtt
    29581 caggtccgag agatccgccg tgcgcgcgac ctgcccttcc ggcgcggcga ggagaaccga
    29641 tttcaggcag cagatgatgt tcatggctca gtctcccagc acgccgcggg cgatgaccat
    29701 ccggtggatc tccgaggtcc cctcgtagat ccgggtcacc cgagcgtccc gccagtagcg
    29761 ttcgacgggg taggccttcg aatagccgta gccgccgtgg atctggaccg cttgatccgt
    29821 gatccgcatg gccgcttccg aggcgtggag ctttgccatc gccgaggcct gtgaaaacgg
    29881 atctccccgg tcccgccgga cggcggcgcg aagcaccatc aggcgggccg catcgagatc
    29941 ggtggccatg tcggcgatca tctgctggat gctctggtgc cgggcgatgg gcacgccgaa
    30001 ctgccgccgc tctttcgcat agcggacgga ttcctcgagg gcggcctggg cgattcccac
    30061 cgcctgggcg gcgatgccga tccggcccgt atccagggcg gcgagaccga tggcgagacc
    30121 catgccttcc cggccgagcc gattctcgac ggggatcagg cagtccgcca gccggatgga
    30181 actcaccggg ttggcgcgca tcccgcagag gtcctcgagg tcgcccaccg agaatccggg
    30241 ggtcccccgt tcggcgacga tgacgctgat tccccgtcgg cccgccttgg gatccgtccg
    30301 agcaaagatg aggcaaaggt cggcgatgcc gccgttcgtg accaggacct tcccggcgtt
    30361 gacgcagtag tgatccccgt ccctcagcgc cgtggcctcg atgccggagg cgtcggagcc
    30421 ggcgttgggc tcggtgaggc agaaggcccc gatcttttcg ccgcgcgcca ggggcgggat
    30481 ccagcgctgt ttctgcgcct ccgttccgaa ggcgaggagc ggggcgacgg ccacgctgtt
    30541 gtggacggtg acgcacagac ccaggccccc ggaggcgcgg gagatttctt cgatgatgag
    30601 gacgtagctc accgtatcca gttcggcgcc gcccagggtc ttcggcgcct ggatgccgaa
    30661 atagcccagc cgtcccatct tgtccgccac ctcccagggg aagctcgcct cctggtcgat
    30721 ctgcttggcg atgggggcga tttcccgctc gcagaaggcc cgcacggcgc gcctgacggc
    30781 gcggttcttg tcggtcagaa aggtgttcat cgggtttcgg gacggcctct gttcaaattt
    30841 gcatgcacgc ttcgttcaaa gggcggctcg atagcacaca ttcgggaaaa cgtcaacggg
    30901 tcgacggggg gtccgttccc cgccccctca gccggccggg gacgtgccgg ccgccgccag
    30961 gaccgccagc acctccgttc cgtaccggtc gatctttccc ggtggaagga gcccttgctc
    31021 ccggaagaac gtcagtgacg gcgtccggct ctgcgaaagg cgcaggagct cttggtcgga
    31081 gagcaccatg aatgccgacc ggtccacctc ccgggccttg gcgaagcgcc actcgacgag
    31141 ccgccggaga cggagccgct cgttgtcggt caggacctca tacccgggga tccgctcgtg
    31201 ggcgcgggga tgcggggtct tctcctgcca gacaacggcg gccatccccg caaagaccgc
    31261 ctcgccctgt tcgatcagcc cgcgctccgc gaggagcgcc gacagggttt catagagggg
    31321 gaagaggaag agcgtgtccc ggatggcata gcgaatctgg gcgtccgaga gcgggcgccg
    31381 gtcccaccgg gaacgctgca tcttcttggt cttgtccaga tcgatcttga gaaacgtgtc
    31441 gatcacggtg gacagggcga ggtgctggct tccgagcagg ctcgccgccc gctgcgtgtc
    31501 gaagaggtga cgaaactgga agccgtagtc ccgcttgagc aggcggatgt cgttgtcgcc
    31561 ggcgtggaaa atcttgagga cggccggatc cgcgaatgga ccgccgagaa acgagaaatc
    31621 cagggaatcg agcggatcga acaggtaggt tgtcgccccg tcacggatct ggatcaggca
    31681 aagcctctcc cggaagtagc gaaaggagtc gtactccgtg tcgatgcaca gggcgcggct
    31741 ttgaaaaatc ttttgccggg cgtcccgcag tttgtcgggg ctgtcgatcc atatccaggg
    31801 gccattcatg gttgcgcgtc tttccggccg ccgcgaacat cctgtctcgg gaagacggga
    31861 cggtttcaca tccccggcgg tgcgtgggag tgtaggagaa aagggcgggg cttgtcaaca
    31921 aaaatggccg tcacgaccga cgggatggcc tcaaaagagc cggagggcca gggggagcgt
    31981 catcaaggcg acgatcagtc ccagcagaac gcaggccgcg acgtccggcg gcgtgaagcg
    32041 cggggtcgtc aggcccgtcc gggggccggg gcggtagccc cgcgcctcca tggcctcgac
    32101 cagggcatcg gcctggcgga aggtccccag gagcaggggg gcgacaaggg cggagacggc
    32161 ccggaggcgc ccgggaagcg ttccgctccg ggtgtcggag ccccgggaac gctgggcgtc
    32221 gcggacctgc aggaacgtct cgtggagcat gggcaggaag cgcagggcga tcgaaaccgt
    32281 gaaggcgagc tccgtgtagg gcacgccgag acgtcccgtc ggtttgagca gccgttcgag
    32341 gcccaggacc aggagccccg gcggcgtggt cagggtcagg aagaggcccg cggagactag
    32401 gcagaggaac tgccaggcga cggtgaatcc ccgcgcgagg ccggtggagg tgagggcgat
    32461 cggcaggccc ggcagggcga acaggaccgg cccgttcgaa aagaaggcat gcaggagaaa
    32521 caggagggcg acgaggaccc acaacgggcc cagggcggac aggaagcggc ggaacgggat
    32581 gccggacatg gcgccgacgg ccgccaagag cgccgtcagg aggatgccgc ccgcgagtcc
    32641 tgcctgaaac agaaggatgc tgaaggcgat ggcccagagg atcttcacgc gcggatcgag
    32701 ccgatggatc ggaccgtctc cggggaggta ctggccggcg ggaatcatgg cggcccgccc
    32761 tccgttctcc cgccggcgtc gcgcccggcc agtaggccct gcagcgcccg ggcggcctcc
    32821 tccaccgtca ggacgtcggc ggggagatcg acgccgtcct gccgcaggcg ctccagcaag
    32881 cgggcgacgg gaggcgtttc caggccgatc ccggaccatg cccccggctc cttcaggatg
    32941 acgcgcggcg gaccgtcgtg gcggatgacg ccgccgtcca gggagagcag gcggtcggcg
    33001 ttcaggaggg cgtccaaatc gtgggtgacg tggaggatcg cgatcccttc gcgctgcagg
    33061 cagtcgagga cgtcgaggac gcggcggcgg ccgacggggt ccaggtaggc cgtcggttca
    33121 tccagggcca ggtagcgcgg ctccatggcc aggacgccgg caatggcgag cagccgcttc
    33181 tccccgccgg agagggcctg gggggagcga tgtctcaggg cgtccaggtc gacggctgcg
    33241 agggctgcat ccacgcgccg ccggatctct gtcggcggga gaccgaggtt gccgggaccg
    33301 aaggccacgt cctcttccac cgtcatccct acgatctggc ggtcggggtt ctggaagacc
    33361 atccccacac gccgccggat ctccggggcg tggcggcggt cccgcgtgct caagccgtcg
    33421 atcaagacct cgccggctgt ggggacgaga agggcgttca ggtgcttcag aagcgttgtc
    33481 ttgccgcagc cgttcggacc cgacaggacg acgtgctctc ccggggcgac gacgaacgtg
    33541 gctcccctca ggacggagcg ggactcgcca tcgtaagtga aggttacggt ttgaacctgg
    33601 atcacgcctc gctcctcatc ttttcaggcg atcccggacc ttgaggaaga tgcccgtcgc
    33661 cgcggcgatc ttgagaacat cccccggcag gaaagggagg atgccgacgg tcagggcctt
    33721 cgcgagtccc agctttccca cgacggccag ttgggcgacg cccgtcccgt agaggcaggc
    33781 cgtgccggcg gccagggaga aggccaccca ggcgaagccg ggccttgcct tcagggcgat
    33841 caggtggccg gcgatccagg ccgccgggac gaatcccagc aagtaaccgc ccgtcggccc
    33901 gatcaggacg ccgagccccg ccttgccgcc ggcgaagacg ggcagaccga tgatgccgag
    33961 caggaggtac acggtctggc tccaggcccc cagggcgccg cccagcgtgg acgcggccag
    34021 gtacaggaag agggtctgga gggtgaccgg cacgggaggg atcgggataa ggatgaaggc
    34081 ccccacggcc gtcagggcgc cgaacagggc cgcacgggtc atgccccgca aggtgatggg
    34141 gccggccgca tccttcatga aacctctcct tcccggcggg ccgatcccgt tgaagccaaa
    34201 cggtgtcacg gattcttccg cgacccgttg ggcggaagcg cgtcggggaa acgccggtgt
    34261 ttaatccgga tcggactcga tccgcgtcac ggggggcgag tatagggaag agcggacggc
    34321 gagtcaagcg attccggttt tcaaaaaaat ctggctattt cccccaaatt atactatgga
    34381 acgtggccct gaacctggtt ccgggccaag agattccgtt ccgccggcgg aagcgcgcgg
    34441 cgggcgacag cgttggaaga ggcgtgtatg aggttcgata ttgcgaagag cggcagcggt
    34501 ctgacctacg agatccgcaa catcgtggcg gttgccaata aactgaaatc ctacggtcgg
    34561 gaggtcgtct gggagaacat cggcgatccg atcaacaagg gggagaagat cccggcctgg
    34621 atgaaagagg tcctggtcaa gattctcgag gaagaccggt cgtacggata ttcgccgacg
    34681 aagggcatga acgaaacccg ggaatacctg gccgacaagg tcaaccggcg gggcaaggtc
    34741 cagatctccc cggaggacat catctttttc aatggcctgg gcgatgccat cgcccgcgcc
    34801 tacagctcca tccgcatcga cgcccgggtc atcctgccgg agccgaccta ctccacccac
    34861 ctgctagcgg aggtgctcca cgcctccttc ccgccgaaca cctaccggat gaatccgtac
    34921 agcggctggt gcccggacat ccgggagctg gaactgaagg tcaagagcca tcacgccatc
    34981 gtgggcattc tcgtcatcaa cccggacaac ccgaccgggt tcgtctatcc cgaggaaaac
    35041 ctccgccaga ttgtccagat cgccaaggag cacgatctgt tcctcgtctt cgacgaaacc
    35101 taccggaaca tcgtctacaa cggcaagcag acggtccccc tttccgacat catcgaggac
    35161 gtcccgggga tcagcatgaa ggggatctcg aaggaattcc cctggccggg ttcccgctgc
    35221 ggctggatcg aggtgtacaa cgccgacaag gatgaaacat tctcgcgcta catcggcgcc
    35281 atcctcagcc agaagatgtc cgaggtctgc tcgacgacgc tgccccagat cgccattccc
    35341 cggatcatgg agcatgcgga gtacgaccgc tacctgaacg agcgggtccg ccactacgag
    35401 aagctatcca acatcgccta cgacatcctg agtgacgtcc cgtgcctgat cgtcaaccgg
    35461 acgaacggcg ccttctacat gacggcggtg ttcaacgagg cgatcctgaa caaccgccag
    35521 gccctgccca tcgagaacga cgagattcgc cagttcatcg agaaactggt gcacgacaag
    35581 atcgagcccg acaaacgatt cgtgtactat ctgctggggg caaccgggat ctgcgtcgtg
    35641 ccgctgacgt cgttcttcac cgccctgccg ggcttccgga tgaccctgct cgacaaggac
    35701 gagaaggaat tcgagcaggt cgtccgcacc atcgccgaga gtgtgacacg ctacatcaac
    35761 tccgccacgt aacgcggagg acattccgtg ccgccggcga accggcgaca cgggaatccc
    35821 agtcctgacg ccaggccgtc tgtcgccctg ccgcgcgtcg atccaccctg tctattccgc
    35881 gttcacttcc tgtttccttt agaaaatgcc ttcatggccg cgaccgcctc tttgaggtct
    35941 ttgacggcaa gcgccatata aagggttata agcaccagga agggaatgcc cacgatcacc
    36001 ccatagacga tattctgccc gttggcgtcg tgtcccacga acgggaacgt gatgccggcc
    36061 caccaaagga agaccagcca gatcgccagc aggaacaccc caatccacac acgccccttc
    36121 agggtctcgg cggagacttc cggattcagt ttacttttga acatggcttg cacctcctgc
    36181 aggctgagtc ggaacattcg ctcccttggc gatttcacaa cgcggaccct cgacctttct
    36241 cgggtcgccg tcgggaatga ccgccggcgc catatcggcc ggcttcgctg atgccgggta
    36301 cttttggtcg tatgtcgttg ggaaaacgct gttgaatggt gcgtaccggt tgaatccgat
    36361 ctgcttgagc ggctgggatg ccagaatggt gacgaacagc atcacgggga tgaaggccac
    36421 ggttcgaaaa tagaaaatct tcttgagggt ctggctgtcg gtatccgaca ggtcttttgt
    36481 gttgcgccgg aaaatgagcc cctcgatggg gcgcaaccag gcaaatgagc ccttctcgat
    36541 gacgctgcgc tcataaatgc cggcgaagaa gaacagcatg aaaaaccagc cgatgtaatt
    36601 gccgacgctg actccccgga agtaaaaacc ggcaacggtc caccgccacc attcattcac
    36661 gacgctgatc ggatccatca gcatatcgag gctgaccatc atgacggagg cgaccaccgc
    36721 cttgatccag atgttgccct gcggcaggag acggtccgaa ataatgaaag cggacaggcc
    36781 gaagatgaac cagccgatcg agatgctcag aggcacaaaa aagaccatgc agctccagcc
    36841 gctgaagccc gggatcaatt tgtccgccca gcagtagtag ttcgcatagg cgaaataatc
    36901 ataggcaccg atgatgacca tggcattctc gacggtcagg gtccagatca gggagcccca
    36961 gaggaagagg cttgttttct ctcttccgat gatctttgtg ccggcccaga agaccagaat
    37021 aaaaaacagg tagagaacaa tttcgaacat ccaggattcg attctcaagt ggggaccaaa
    37081 gtacatcttt ccctcctttc tgacaggggc gtctttttca agccgcgcgg gttgcgaatt
    37141 caagatcagg tgttcgtgaa gcggtttcct tgcccccgcc tcacgcccgc ctgccatgtt
    37201 gatgtcgagc cggtcgccga ccgggaaacg atccttgcgt ggacccccac cgacgtgcgc
    37261 ccgatcattc cttgggccgc atgtccacca cgcgcgtcgc tttgagcgtt tcccccggat
    37321 tgaagatcgc cgacctttcc tcgaagatga ttttgggcgt caattccacg gcccgcttga
    37381 ccttttcctt cagcgttctt tccagttcgg ccagatccac ccccttgtcg gcgccgatac
    37441 gcaattggag caagtccatc gaatagggat cgtccgcgtt ttccttcgtg accacaaact
    37501 ggtattcgat tacaccggcc gtattggcga ccgcgtcgcg catgatttcg ggattgatca
    37561 gggtgccctt gaccttcatc agttccttgg tccgcgttcc gtaggtgctc cccaccgccg
    37621 caatgatccg ctggtcgttt cggccgcagt gctcgcaagg ttcatccgta atggcgacaa
    37681 tgtctccgac cagatagcgc agcagacagg ttccccgccg gtcgaggtgg gtgatgcaca
    37741 aaagccccga ctcgccatcc ggtttgcggt ttccctgttc atccacgact tcgaagtagc
    37801 agagcggtcc gccgccgtgc gcccctccca acggccgaca ttcctggaac gcgctctgcg
    37861 cttcggtaaa cccgaaggcg ttgctgacga agacctcgcc ggcccccaag cgttgcagcc
    37921 tgcggacgat gtcatcgcgc atgcccatgg gaacggcctc tcccaaggcc tggacggact
    37981 tgacggacga aaaatccctc ccctgttctt cggcgaccat gatgagtctg cggacgtagg
    38041 acccgattcc ggagaggaca ttgaccttgt aacgttcgat gtcctcgatg gattgctgca
    38101 aggaacggtg aaattcgtaa ggaaagccgg gcaggggcgc gcccgtgaag ccccagcaca
    38161 tcctcagatt cgccgcggcg gcgaagtgga cggtcttgta gtagcccata tgcgggatga
    38221 agtggtaggg aaagagattc atgaccatgt cgcccggttc catgtagcag atcttgcaac
    38281 cgatctgaaa catccagcac tgcatgaaca tgtcgtacgt ggtgttgaag aacggggagg
    38341 gcatgccggt tgtcgtgccc gtcgtataga gcacctcata catgatgtaa tccagcatgt
    38401 tggccgggtc catatccagt ctgaaggaca tgccgcggtc cttcatgaat tcctccttgc
    38461 tcgtcagcgg gaccttgacc aggtcgtcca cggttcggat ggacttggga tccaggcccc
    38521 attccttgaa tttggcgcgg tagtgaggac tgaacttttc caggcgatcc atctgtttga
    38581 ccaggttttc attctgcatg gtcctgagtt tttccgccgg gaagaaaagc gcatccgtgg
    38641 ccatcgtggc ttcccacgcg ggatggcttt tgatccaggc cctgatttcc tctttgttct
    38701 tcggtatcgt gaacggtttg ttcggcatct tttccctctc ctcaaaaaat gatcgtatcg
    38761 gttttcccga cccccgaccc tctgggggaa gcaatatccc ccggtttgcg cgccgtccaa
    38821 ccggccggcg ccggcgtgct ttcattccgg gacgtctcgc ctccgcggtc ccgcaaaaaa
    38881 aggggacccg tgggtgggcc ccctcatttc ttcatggctg tgcggagtca cgtttgaccg
    38941 ccatcattcg accctcagcc aggtttgccc cttgcagatg aacgaaatgc atccggaggc
    39001 cttcagcttc ttgccgcctt cgaccacctt gagcttgccc ttgtagatct tcccggagcc
    39061 gggatcgagg atggtgccgc cggtgtattc atcgccgtcc gccttgagcc ccttgatgaa
    39121 gaccatcccg acgatgggct tccccttatc ggcgccctcg cacttgtcgc agaccttgtt
    39181 cttgtccgcc gggtttagca tttcgagaaa tttcccgtaa atctttccgt tctgctcgta
    39241 gacctcgacg atggacttgg ccttgccgtc cttttcgtcg atcgtcttcc acttgccgat
    39301 cggtgacgcc gcccagagcg gtgccgcggc gaacaccaac gccgccatga tgaccgtctc
    39361 caacgcaatt ctcttcacct tcaaacctcc tcctccagaa ttgagtctca gccgccggtg
    39421 acagatgacc ttccagcacg gatggacgag actttgcacg cacctcgaaa acggccggcc
    39481 gaggcgccgg cgtccgggag cggtgtcggc gcgaatcgcc aaggcgtatg actgaccggt
    39541 cgatcgcgtc ggtggacata gacgaaatcg tttcccgtgt caagaaattc aggaacgttt
    39601 cccattcaga tctgttcgtg aacgatgcgc cggatctcgc gcttcaagat cttccccagc
    39661 cccgtcttgg gcagggcgtc gaagatgaag atcttcttcg gcgccacgaa ggggtggagg
    39721 tgttcgccca ggtaggccag cagctcctcc tccgtggcct tcatcccggg cttcagggtg
    39781 acggccgccg ccacctcctc gtgatatttg gggtccggga tggcgatgac ggcgcactcc
    39841 cagatggcgg gatgcttgta gagccagccc tcggccacgc cgggatcgat attctcgccg
    39901 cccttgatga tcaggtcctt caggcgtccg gtgatgaaca gatcgccgtc ctcgtcgagg
    39961 cggcccaggt cgcccgtgtg cagccagccg tcaatgatgg ccgaggcggt ctcctggggc
    40021 ttgttcaggt agccgttcat gaggcagtcc ccccggcaga cgatctctcc ctcctcgccg
    40081 ggcggaaggg ccttgccctg aaaatccacg atcttcagcg cgatccccgg cagggccgcc
    40141 ccgacgctgc cgggcttgcg cggccggtcc agacgctggc gggtgatgga cccgcccacc
    40201 tccgtgcagc cgtagccctg ggtgatctcc atcttgaagg cccgctgggc ctggttcagg
    40261 gtttccatgg ccagcgggcc gccggagcag atcaagagca ccagggacga caggtcgtcg
    40321 atctccggct tgtggaccat gaggagatag atgagcatcg tcgggacgac ggagacgtaa
    40381 ttggccttgt agtcgcggat gacgtcgagc acccggcgcg ggtcgaaggg ctgcatcacg
    40441 acgcacaccc cacccccctt gttcccctcc gtgatgctca acaccccgta gatgtggttc
    40501 atgaggacgg tggtcaggac ccggcctccg accttcccgc cccagaccat ctggccgctc
    40561 gtgaactgga acctgaacgt ccggtggctg agcaggacgc ccttcgggaa gcccgtggtc
    40621 ccggaggtgt acatcaggac ggcgatttcg cgatcgggat cgacgtcgac ggcgtcgccg
    40681 cggccgtcgt cctccgtcag gaggctttcg tacgggacga tgttgtcgcc ttcgccctcc
    40741 ccgatcagga cgatccgctc gacggacggc aatcccttcg ccgcctccag gaccttcggc
    40801 agcagattgg cctccgtgag gatgaccttg cagcccgagt ctttcaggat gtatcggatc
    40861 tccgccgccg tgagcatgta gatgatgggc aggtaggtgg cgcccatgcg gttgatgccg
    40921 ttgatgatct cgatgatctc caggacgttg ctcaggaccg agcccacgat gtccccccgc
    40981 ttcacgccga tcttctgcag ccccacggaa aagcggcggg cgcggacgag gatttccctg
    41041 ttggtcagga tcttctcgcc ctcgtacccc aggtagatca ggtagtcgta ttcgccatag
    41101 gtttcgatgt tcaggtcgag aatggagctg atgttgctgt tttccatgaa gcgctttccc
    41161 cccttcggtt gcttatggcg ccggcttcct gacggtgact gcaaaaacca tgggtgggaa
    41221 tgctcacgcc ccggcaagcc agaacgggtc gtcccacgcg tagggaagcg cgacgatggc
    41281 gccgggtgtg ttgatctcgc gggcgccgcc cagggcgtcc ttcaggatgg ccgtctgatt
    41341 tgctcggtcg aacggtttgc ccgaggtatg gccgagcggg tagttgataa aaaaggtccg
    41401 cggcggcttg acgagcgtcg tcaggtcgta ggccgaggac agggacacgg tcgggatgcc
    41461 tttctcttcg agggttcggg cgatcaatcc cacggattga tggcagagtg gtcaggcggg
    41521 caccagcagg acgaggtcgg ccttcatcgc ctcgatgcgg gccgtcatcg cggggatcat
    41581 ctcgccgagg aactgggtct tgcggaagac gcggcccatg aaggtcaggt ggaacggcgt
    41641 gagcccgccg atgaacccct cggcggcgag ctcccgcagc cgctcgatgg gaaagacgca
    41701 gttgacgtct tgttccgcgt cccggtgatc gtagtagtcg tggctgatcg ccaggtcgcg
    41761 tacgtccgtg tccgcgggga tctcgcggaa ggacaggtcg tttttgtcgg gcttgaacgg
    41821 ggcctgatcc ttgcggtaca tgccgctcga gctgatcatc gcgacggcgc tctccgccag
    41881 gggcttgacg atgggcgtcc aggggacgga atcgttgacc gtccactggt aagggggttg
    41941 atccggaaac atggactgca cgacatcgac gtacttgagg tacttcatcg ctccctcctt
    42001 ccggtccgaa tgtatgcaaa atctaaccga tatctctgaa agatgccaac aaaaagtcgg
    42061 ggcgtgaagg tttcgtcgcg aattggccga atgatcacga atgatccttg acgacggggc
    42121 aaaaatcggc caaagataag taacaacgtt tggaaaacgt ccgcgggtgg cgaagggcga
    42181 cgaggccttt gcggggaagc ggcgggtatt ccatgcgcac ggccctgcga ggaagagaca
    42241 gggaatgaag gatggaatga ggatgacgaa ggaaagcaaa cacacgctgg ccgccctgtg
    42301 gccgaccatt cgacaggccg cctggacctg catcctgctc gccgcgggca gtgccatctg
    42361 cgcggcggcg gtcaacggta tcctgattcc ccgggagttc gtgagcggcg gtttcacggg
    42421 actcgccctc gtcctgcatt acctctggcc cctgcttccc gtgtccgtcc tgtatttcct
    42481 cttcaacgtg cccctgttcg tcgcgagctg gttcttcgtg ggccggcggt tcttcgccta
    42541 cagcgtcgtc gggatggcgg tatatgccgt cactctggac tgggtgcggc tcccgatccc
    42601 ggtgacggac cccctcctct cggcgcttct ggccgggatc ctgatgggaa tcgggatggg
    42661 gatcatcctc cggtccacgg gctccgccgg cgggacggac atcctgtcgg tcatcctcta
    42721 ccagcgctgg tccgtgcgtc cgggggcgac gatcctggca tcaaacctcc tcattctcat
    42781 cctctccctg attttcttct ccctggagat cgtcctgtac accctgatct acctgtacgt
    42841 gtcctcccag atcatcgaca tcgtcgtgat gggtctgagc cggcgcaagg ttgtcttcgt
    42901 catctcgcgc gagtgggagg ccatctccag acgagtcctg aaagagatcg accgggggct
    42961 caccatcctg cacggcgaag ggggatactc gggccaccgg cagcaggtcc tctacacggt
    43021 cgtgaccttc cgggaactgg ggctcctgaa acgcatcgtc cgcgaggctg acccggcggc
    43081 cttcgtcgtt gtgaccgata cccaggaggt catgggccca cgcatcggaa accagccccc
    43141 ttggtagggc aggcggcggg gtcgccttct cccgccccgg aggcgagatg gaagcccgcc
    43201 tcaaggtctg gccctggtcc ggtgaggaag tccgatctgc cccgctgagg tgccgccggg
    43261 cagggcgtaa aaaaggtgtt cagaaatgcg aaattcaggt gaggaaaaat cggcttgggg
    43321 ttaaggtcga tcgtccgtca aaccaaatca aggaggaaca catgtctgtc acagaaaaca
    43381 aggtcatgac ggcgaaggag gccatcggcc gcttcgtcaa ggacggtgac cacctggtca
    43441 taggcaacta cacggtgggg acctgcgccg ccctcgtttt cgaggtcatc cggcagcagc
    43501 ggaagggcct gacgctctac tcgcagtccg gcgtcttcga cgtggaggtg ctcgtcgggg
    43561 gcgggtgcgt ggaccggctt gtgtccacct acgtctaccg ggccggcggc aaggagggcg
    43621 gctcggccgt ggaacgcgcg atgaaggcgg ggacgctcga aatggaggac tacacgaact
    43681 tccagtacaa cgcccggctc gtggcgggca tgcacgggtt ttccttcatg caagtcctcg
    43741 agggcgcgat ggtcacggac ctgttcaaca agcgctcgtt tttgggcgac gacaagtacc
    43801 gggtcatccc ctgcccgtac acgggcaagc agatcctcac cgtgcccgcg gcgaacccgg
    43861 acgtctgcat cgtgcacgtc cagcgggccg accgcttcgg caacgcccag tactggggcg
    43921 ccctgggcag cgtcgccgcc gcggcgctgg cgagcaagcg catcgtcgtg tcctgtgagg
    43981 agattgtgga gcacgacatc atccagtcga gcccccacct gaccgtcatc cccggctacc
    44041 gggtggacgc ggtcgtggag atccccttcg gggcgcatcc caccgaggtg ctcgggtact
    44101 acaacctcga ccgcctgatg tacgccctgt tcttcatgtc ggacatgaac ggcgacgggc
    44161 tcaaggcctg gatggacgaa tgggtctacg gctgcgtgga ccggcacgcc tacctggacc
    44221 actacatggc caagcacggc gccgagtcgc tcgacgccat ccgggcgaag gcctactact
    44281 ccgcgccggc gaactacggc gccgcctaca cctcgtgctg ggacaacgag gacagggaac
    44341 gcagcatggg cgtcacgctc gcggagatgg aaagaatcct ggaggaaagg ggactgctca
    44401 atggctaaga aatataccct cgacgaactg ctcatcatca cggccgcccg cgagatcaag
    44461 gacggcgaga acgtcatcct cggggtgggg cttcccacca ccgccggcgc catggccaag
    44521 gccctgcatg cgccgcacgc caccctcatg atggagtccg gcatcctgga catcgagccc
    44581 ctggtgccgc cgaaccacat cgcggatgcc cattcatgcc ggggcttctc ctacgccacg
    44641 gacctctttt ccatgttcac catgacctac cggggctacg tggacgtgtg cttcctgggg
    44701 gtggcccaga tcgaccgctt cggcaacatc aacaccacgg tcatcggcga ctactaccag
    44761 ccgaagatgc ggctgccggg atcgggcggg gcggcggatt ttctttcgta tgccaagaag
    44821 accattctca ccatgcgggg cggagagttc gtcaacacgc tcgactattt cacctcgccg
    44881 ggctacctgg acggcgggga cagccgggac gcctcggggc ttttcccgag ggggtctggc
    44941 ccggtcatgc tgctcacgac gaagggggtc ttccgcttcg acgagcagac caaggaactg
    45001 gtgctggcca aggtccatcc cggtattagc gtggacgaca tccgcaagga catcccctgg
    45061 gatctgaaga tcgcccccga tctggccgag accgaccggc cgacggacgt cgagatcgac
    45121 ttcatccggc gcttcgcccc gaccgaggcg gtcggccgga agcttatgta cgagctcgcc
    45181 atcgcgcaca gcttcaacaa ggccctggcg cgggggaagt gagcgcctct cccgccgcga
    45241 cgccggtcgc gcaagggtta ggggtgggcg aggggtggag cgtggagggg agggcaccgc
    45301 atggaccggg cggctatggg atccgttgaa cgcagcgctc gtggtcttgc cgcggctccc
    45361 gaatgatgag gttgttgttt ggggtggagc cattcatgcc ccgcgaggtc cggcggactt
    45421 tcgaaatctt gacgagacgg ggatccgtcg acctcaacat acgcttatgg ttccaaacat
    45481 gcccccatac gttgcaatgt cttgaacgtt ttccaaaagc ccagcgggga tggccgtcgc
    45541 cgcgttgatg gcgtcaacaa cctcggcttt ctggcgggct ctaaaatgcc gcccgagata
    45601 aaccgacaca acgacacctt tgtcgaatct aaatttgact tttctccttt tggggggaag
    45661 cttacgcctt caaagcgtcc ctcaatatgc tgaggttccg ccaaatattg aggccaaaca
    45721 aatcccctta tgctcctcgg ctattctctc cctttaacct agcagactgt tattcctgat
    45781 gaatcgaaac atctgatttt ccctaggcca ttggcgggct atttgcggct acatatgtca
    45841 atccgattgg ataatttaac gccgccggag gaaagagggg taattaccat ggatcaggga
    45901 acacagcatc ggggacagca ggagcttcag gcgataatcg ggggcatttt tgactacgtt
    45961 ctcaggaagg acaaagagaa cgccatcctg agtgcgtgca ggatggaacg ccccacggag
    46021 aagtcggatg ctgggcatac taaaccatgc gcctgttttc aatgtttatg accagtatat
    46081 catatcaaac tttacaacta acagcgacac tgacagtgag aaggtttccg ttgcttcccc
    46141 ggaaatggca acttctcttc cggcgtagcc tctcgggact cataatatca acaatctttg
    46201 tggtattgat gccacaggaa acggaggcaa caatgagaac acacaccatg acaaaaccgg
    46261 ctgaaaacgt actggcactc actcaattca tcctcgaacg cccctctctg ttaaacacaa
    46321 atgatttcat acacaccagc catcatgaca ttgtaagatg gtttatcgat tgagatgtga
    46381 cacagatgag cagaataaga acaataacag ttcagaaaga acatcacgag ggaaggcttc
    46441 tcaaaagttt tctcgcgctt aagtcggata accttgcagc cttcacctac cgactgttca
    46501 gctgtcgcca ttccgcagaa ttgatgatga cggaatgaaa cgaactgcct gaaagttgcc
    46561 ttgcggatta gagatctttc agcaatctgt ttgcgggcag ctcctcaatg gttttgcgga
    46621 atgtttcgag cgtttcagat atcatggaaa attcgtgcgt tttggcaaag gggattgtgt
    46681 tgatccccct ttgttccatg gcgtcgacga catgctggat cgtcaacacg ccgatgtcac
    46741 gggcaggctg gaatccccgc tcgcaatcgc ctccgatgtc cgtagcggag agaatgccgc
    46801 tttgggtcag atcgaagagg atgtcgttga caaggcggat cgggatttca agctcgtgcg
    46861 acaccttcct ggcagtcggg ggagcttctc cccgggcgaa gttttggatg atgtgctgag
    46921 cgatctgaag tgaaagcagc ttcttgagcc ggcggctgac ctgaagtgca tctggttcga
    46981 attcataggt gttcacattc tgatgggcaa aggacacttc ggcgccatac aagacgatca
    47041 accagctcag ctggagccag atgaggaaga gcggaaaggc agcgaaactt ccgtaaatgg
    47101 cgttgtattt cgctaccccg atctgaaagg cgatgtatgc ccattgcaca acctgaaaaa
    47161 tggtgccgga aacgactcct gccagcagag ctgacgtaaa gcgaaccttc gtgtttggca
    47221 tgaacatgta caggaaagtg aacaaccccc ataggaggct gtaggggagc agtttcatta
    47281 aaaacaagac gacggggctg aacgccccta ggattgttat cctttccatg atcaaggtca
    47341 cctgtgtggt gacaaaaatc gtcacgctgc ttgagagaat gatgagaatc gggcagacca
    47401 gcatcagcga caggtagtct ccgaatttcc gaccgaggga gcgctgctct ttgattcccc
    47461 agatatcatt tagggattcc tcgatctggc ccagtacctt gatcacagcc cagaaaagaa
    47521 cgatcaggcc gatgccggcc aataatcctc ccttggtgct ttccaaaaga gagtgggaga
    47581 actggatcac attgttgaga atctcttcgt gaccggtcag cttgctgcgc aactgcgctt
    47641 ccagcacctt ttcgaagccg aaccccttgg caattccgaa ggccatggcg gccaccggca
    47701 cgattgagat cagggagtag aaggtgagtg ccgatgcccg gagctcgcac ttgtcttcat
    47761 tgaatcccct tactgaaaga atcagcaccc gcagcagcgt taggaaaaaa gatttccccg
    47821 gaggaaggtt tgtgcgtcgg atacgccaga catcgtgttt gatgaagttc actgcctttg
    47881 tccatatagc ggccatggcc ccttccccct acgctattta ttaacccaac aattaagcat
    47941 ggctaacaat tctgcttgat gcccgtattc attagatctc tccataatgc ttcgccgtat
    48001 taagttgccg gtttaatgtt attcgcaatc gagatgaagg caaatggtta taaagtagcg
    48061 actttcaact gttctcatcg cgcaatcatg atcacagttt ccaacatgtc gattatttcg
    48121 ttcacccaat gagtcttcag agacaggata ctgcaagatg gatttattat ggatttattt
    48181 acgttaggaa gctccaagga tcaattatca taatctcacc cccgtgcctc tggtgcaggc
    48241 ggccaaagaa cagctgcttt ctggattcag ccgcgctagt tgccgaagac agtcttcagg
    48301 agttccgtca cccttgcttc gggattggtt cgtatcttct gttcttcttg agcgacgacg
    48361 agaaacaggc cgtcgagggc tttgttggtc acgtagtgat cgagatccat tgcctccttg
    48421 gagacaaacg ggagtgtttc ataaggcgcg atcatgtcct tgtaggcctt tgccaccccc
    48481 accttgttca tgttcgacac gaccacaggc ttgaactcgt catagagtct ttccgacgtc
    48541 ttgtctttga aatactgggt cgccgccgta tcacccccct gaataatctt ccgcgcatca
    48601 tcgattgaca gggtcttgat ggcatccgca aaaagggcga tggccttggg agcggccgcc
    48661 tccgccgcac gattcatgct caggacaaga ccgtcgacct gttcctgata gcccagccgg
    48721 gcgacgacat cggcaaccct ctggatattg tcgggcagtt ttatcttcac caacggattg
    48781 ctgaaatagc cgtcggtttt cgacaacgcc ttgacggcat tctctgtgcc gatgaccagg
    48841 gcctccctta atccggaaac ggccgttttc tgatccggaa cggaggccgt ctcggcgctt
    48901 ttagcgaccg gcagctcttt cagaatatcg gaccatatgc cggcttccgc caaaccgggg
    48961 atgccggcaa cccagaacag tatgaagaaa acagacccta tctttttcat cactcatcct
    49021 cgtttcaagt tgatggtccg ggtgtgggag gccctccgat tccacacgtt cctctatcgc
    49081 acaagcggac cgattgtcaa cgacaagtcg tccgctgaaa atcccgtttt gatcgcactt
    49141 cgggcagaca cctccggcat gtactgacgg tcgaagggtc ctgcttgata taccctccca
    49201 tgtcggccat tcggtccatg aattcctgaa taagccctcc cttccccatc atcaccggta
    49261 tcgggttcac cagataggaa gtcgcggtgc cgccgccttg ccgcgacgaa gcaagcgcag
    49321 cgaatatccc ggacgtaccg ccgccgcaga cgaggacatc aacgcaggca aaaactttta
    49381 tttctcttgc ttcttcaaag aaagtttcgc tcatggtcat cccctccttc gattggcttt
    49441 ccagccgcgt catgggtaga cacataaaaa ccgcgaatgc aaatgcggct aactggtacg
    49501 gataagacct tgcttttctg ggaagtgagg atggggtagt cgcgatgtcg agagcaaata
    49561 gaggcgtccg ctttgtctga tcctctggag agaagacttg gaggtcagag aggacggcgg
    49621 cggacgtttg cttccccggt atttgacaca aaaaccgcga aaaaaagcca cggtcggccg
    49681 aacttactca cggatggttc cgggcagccg gaatcaatga gatatcggca attcctcctc
    49741 tgtctccggc tgatcgatca ggaaggcctt gacacgcgaa ccgacgggag cgtcgcccgt
    49801 accgatgggc aggatgagca gagcgttggc gccgaccaac gatttcagcc ggccggaact
    49861 ctgggagccg gtgctgtagg cgatgaaccc gtcatgatcg gctttgacaa cggcgcgttg
    49921 aaactcggtt cgatccggat cgtgtcgcaa gttttccgcc aagcttacct ctacacacgg
    49981 gcgccacaaa agattcaatc cgctcatcag gcgcagcgcc ggtcggacaa attgctcaaa
    50041 acagaccagg gacgatacgg gaaaacccgg caatccgaat accggttttc cctgcaacag
    50101 ggcaaaggtg aagggcttgc ccggtttcat ggccacacgg ccgatcagca cgtcgccgag
    50161 ctttgccagc aacggcttga ccagatccag ttttcccatg gaaacgccgc ccgatgtgat
    50221 gaccgcgtcg ctgttgttca aggcctcaac aagggccagt tcaagttttc cgctgtcatc
    50281 acgaaccgga cacgacgcgg ccgtcacttc aaatccgcag gctgcggcgg cgacggaaag
    50341 cgtgagggaa ttggagtcgc ggatttggcc cggccgcagg gtttcatcgg cagccaccaa
    50401 ttcattgccc gtggacatga tggatattcg cgggcggggg tgaacccaaa cctggaattg
    50461 accgagtgcg gccaggagtc cgatctccgc cggtcccagc accaagccct tcggaaggac
    50521 ggtttccccg gcggccatat ccaaaccgac gggtcgcaca ttttctcccg gtttcggaat
    50581 gaccttcaag cggacaatgc cctcgttttc cccggtttct tcaaaaggga ccacggcatc
    50641 tgcgccggtc ggtaggggtg cgcccgtcat gatcctcacg gtcgttcccg gctcaacgga
    50701 caggtcaagc ttgacccccg catcctgttc gccggtgatt gatttttcca gggaggggtc
    50761 gctgctgatc accgcgtagc cgtccatgga agccgcggcg aatggcggca tactgtcctt
    50821 ggcgataata tcttccgcca ggactaagcc ggccgcgcca accagcggct tgcgaaccgg
    50881 tggaaggggt tcaagcgccg aggaaacccg cagccaggcc tccctgatag acagcattgg
    50941 atagtcggaa ctgcgtttgg ctcgttttac cgtcgtcatg ttgtcccggt ttccgtgttg
    51001 cctcagtcca cgccgatcat cacgctggaa gccttgatga tcgcataggc cttcttgccg
    51061 gttttcaggc cgagtctctt tgcggaatct ttggtgatca tcgaaacgac cttttctccc
    51121 cccgcaagct ccaagagcac ctccgtgttc acggttcctt ccgagacctc gatgactttt
    51181 cccttcaaca cgttgcgagc gctgatcttc atggcaagcc tccttttgtt gtctgttgat
    51241 ccctctgtaa atccccgata agtccgaagc tcgggtgaat gaacccagtc cgcgcggtcc
    51301 tcaaacaccc ccgatatccc tatcccacgg ggcagaccga ttgtcaatga caagtaaccc
    51361 gctgaaattt cgttctgatc gcattccggc cggactttcc ggcctgcgcg tgacgattgc
    51421 ggattgaaag attgcgcgtc gatccgttca tgatgatcct gcgccgaggg tgacggcggc
    51481 cgcctttgcg tcaatggaag gcaagcggtc gttctacctc ggcctcagga agcggcgccg
    51541 gttctccatg aagcttttgt aaatccgata gtgctccgtt tcttcatagg ccaccgtctc
    51601 tatgggcgct gcatcgaagc tgtagatgac ggcgccgggg cacgccagca gaagcggaga
    51661 atgggtggcg atgatgaact gggcgtctct gccgtcgtcg gcatttgtca ggatctccag
    51721 aaggtcgatc tggctgcgcg gggataaggc cgtttccggt tcgtccagaa ggtagagccc
    51781 cttcaggcgg tagcggctcc ggaaaaagga caggagcgac tggccgtgcg actgggtcag
    51841 gagcgacttc ccgccgaaat actggagctg gaccgggtct gccgccgccc attcgtccag
    51901 aatctgggcg aagtggtgaa agatggccga cccgaagtac gatccgggca cgcggcccga
    51961 cgtccacgtc acgctcaggc agcgatgaaa ctggtcctcg tatggattgt attcgaaacg
    52021 ggaccgcgac tcatcccgcc agatatgtat tccgcagctt tgggcaagcg cctccaggag
    52081 ggtggatttg cccgttccgt tttctccgac gaaaagggtc acggtcgttg tgaaggggat
    52141 ggattggggc tgtgaaaaaa caggaaggtt gaacgggtaa cgatcccgca ggggatactt
    52201 gtccggatga atcgtgacgt tttcgagatg catgttctca atcggggcga attttcataa
    52261 gcgccgtccg tgatgaagct ttccgtctgc cacggcgccg tgtattgctc ggggccggaa
    52321 ctatagagag gtcggtcgga cgtgtcaagc aaagatgtcc gcgggctggg gcatcgatgt
    52381 cgacgcggcc gtgacggcga tcgttcttgt cagaatgggg ggaagccata ggcccaggcc
    52441 gccgccgtga ggaggaccgt ggattcggcg atctcgcacg cggcgcccag ggtgtcgccg
    52501 gtgaagccgc cgatcttgcg ccggctgtat gccgacacgg ccgccgtggc cgccagcgcc
    52561 agaacggcgg cggcgaaccc catccaccgg gcgagaaggc cgcccgcgat cacgagaaaa
    52621 cacgcgcccc agaccgcatt ggcccgggag cggccgctga ggaagcgcga ggccagtccg
    52681 ccctcggggc gggtataggg gagggccgtc atcatgacca gcagggcgac gcggccggcg
    52741 acgggcgcaa ggaggatcag ggccgtccgc cgcggaagct ccagggtcgt cagggcgccg
    52801 accttgatca ggatgacgaa gacgatcgcc atgaccccca tcgtgccgat gcggctgtcc
    52861 cgcatgatgg cgaggatcgt ttcccggtcg cgggcgctga agaatccatc cgccgtatcg
    52921 gcgagtccgt ccatgtggag gccgccggtg agcttcacca ggatgagcac ggtcagcgtg
    52981 ctcgcgatgg cggagggcag gatctgcgag accaggaaat cgaatagggc ggcgagggcg
    53041 ccgaccagca ggccgaccag ggggaagaag ggggcgcacc gctccaggtc gaccccggcg
    53101 ccctgaaaac gcagggggac gcgaaggacc gtgagaaact ggagggcggc gaaaaatgcc
    53161 ttcatgactc cgccttggag acgttcgcct catcgaaggt cgcgacgtcg gtgaggacac
    53221 gcaccgcggc ctccaccagg tgcagggcca gcgccgcccc cgttccctcg cccagacgca
    53281 ggtccagatc gaggagcggg cgcctgccca ggcaatccag agcaatccgg tgacccggct
    53341 cgagactccg gtgggaggcg atcatggcgt cgagactccg gggggcgatc cgggcggcga
    53401 ggatggcacc ggccgtggag atgaacccgt ccacgagaac gggcttccgc tgcgccgctg
    53461 cgccgaggat gagcccggcg atgccgccga tctcgaaacc gccgaccgcg gcgaggacgc
    53521 cgacggggtc ggcggggtcc ggggcgttga cggcgagggc ccgttcgatc agggcgatct
    53581 tgcgccggag gcccgcgtcg tcgatccccg tgccgcgccc cgtgacgtcc gcgacggggc
    53641 gaccgctgta gagggcgacg atggcgctgc tgggggcggt gttgccgatt cccatctcgc
    53701 cggtgccgaa gatgtccgtc gtcggcccca ggtcccgggc cacggcgatg ccggcctcca
    53761 gggctgtcct cgcctgttcg cgcgacatgg cgggacccgc ggtcatgtcc cgggtgccgg
    53821 cggcgatccg ccgcgacaaa atccggccgg cgtcggccag atcgctcaaa tcccccgcga
    53881 cgcccagatc gacgacgacc acctccgccc cggcgaggcg ggccagggcg ttgattcccg
    53941 cgcctccccg gacgaaattg cggaccatct ggacggttac ctcttgcggg taccggctga
    54001 ccccgcggac ggcgacgccg tgatccgccg ccatcgtgac aatcgtcttc cgggcgacgg
    54061 gcggcggcat gctcccggtg atgccggcga gatcctccgc gagatccatc aggcggccga
    54121 gcgcccagtg gggcatggag agctgctcca ggcgcgcgtg cgcgcaggcg cgggccccgg
    54181 cgtccgcggg cgggatgctg tttacgaccg tttccaggag gttcatcctc tttccttttt
    54241 catccgggat ggcgaccgat ggcctgatgc cgatccgggg gagaatgccg gttgaccgcg
    54301 caggatctca cggctcccgc cgatgcatgt ttcccccttg aggaacagag gcaggccgct
    54361 gaccatcagg acgaccgttt ccgccgcctg cgccatgacc tggttgcacc gcccggccaa
    54421 gtcccggtag agccgggcca ggggggtgtc cggaacgatt cccagaccga tctcgttcgt
    54481 gacgaaaagc accgttcccg acagcgtttc gcaggtgcgg atgatgtccc ggcaccggtc
    54541 ggcgatgtcg tcttccgaaa cggcccggcc ctggcgttcc gccacgtaca ggaggttgtt
    54601 gacccacagg gtcaggcaat cgacgagcgc caccccgtcc ccgccccact gcagcagggc
    54661 cccggtcagg tcgaattcct cctcaatcgt cgtccacgcg gcgccgctcc gcgcctcccg
    54721 gtggcggcgg atccgcgcgg ccatctcgtc gtcgagaacc gggcaggtgg cgataaaggt
    54781 cctggggccc gccgttgcct cggccgtcgt ctgggcgtag gcgctcttgc cgctccggcc
    54841 gccgcccgtg acgaggatca tccgcgccat cagcaaagct ccgccaggcg aacgagcgaa
    54901 tccccgaggt cgtcccgcga aaagacctcg accgagacgc cgccgcgata ggtttcgaga
    54961 aagccgcgca ggaacgacca cgccgcttcg ggaacgtctt tgagggagcg gtgatcgcac
    55021 ccaccgtcga ttccgtggag atggatcatg accgttttgt taccgtacgg cgtgaacagg
    55081 tccgccatcg ggagcgactg tctcaacagg tgccccgcgt ccagacagac attcagctcg
    55141 agcgccgcca cgatcggcgc aacggcggcg aagggggctt caaggttttc cacggccagg
    55201 cgtcgtcggt ccacgccggt gtcgaaaagc gccgcgagcg acgacaggca gttctcccgc
    55261 cagcggtcca tcgcggggct tccattcccg gcggcgggtt gaccctccag gtggaggatg
    55321 taggccgttg gcgccagggg cagggtccgg tcgtaaaact tccacagggt ctcgcccgcc
    55381 gcccgccgga ccgccgcgtc gggattgccc agggccagat ccgtcggcag atgaacgttg
    55441 taactcaggt cgtacgcgtc gccgaggcgc gccagttcgc ggatttccgc ctcggtgggg
    55501 agattttcgg cgaaacggct ttcgaagagt accagctcga tttcgtccac gagcggcgcg
    55561 agaaagcgga cattgggaag gatcggcgcc gggaggatat aggaggtggt tcccagacgg
    55621 aacgggaagc aacccttcag gtccggccag cctttatcca tcacgacatg cctttcattc
    55681 cccggtcgtc ctccggagcc gggatgacga gggggcaaat cgagattcct ttcgggcggg
    55741 agtgcggcct cgaacgggag gggattcgat cgccgacggc cgtcccgatc ggatcgactg
    55801 cggcgtagca cgcgccggaa cggcttgtca aggagcggtt gacaagcgtt tccgcctggc
    55861 gtaagcaggt cagtcaggag tgatcggcat gtcatgatcc cacggaaaat tctccccgga
    55921 accctgatcg ggttcgcact gctgtttctt tgcatgcacg ccgcggcggc cgacccgccg
    55981 cgacggatcg tttccctcgc gccgaatcta acggaaatcc tcttcgatct ggggctggcg
    56041 gaccggatcg cgggcgtgac gaactactgc ctcctgccgc cggccgcgcg cgggaaaccg
    56101 aaagtcgggg ggttcgtcaa tccttccctg gaggccatca tcgccctgcg gccggacctc
    56161 gtcctgctga cggacgacgg caatccgcgg gccgtcgcgg accggctgcg ggcgctcggc
    56221 atccgcacgc acgttttcaa gaccggccac atccgggacc tgccgcgtga gatccgaagt
    56281 ctgggggatg tgctggcggt gcccggtcgg gcccgcgagc gggcggagac aatggcgcgg
    56341 acgtttgaac gcatcgagaa ccgggcccgg acgcggggcg ccccgcggat gcgcaccgcg
    56401 ctcttcatca tccagccgga gccgctcatc gtggcgggga agggcacgac catggacgat
    56461 gcccttgccc tgctcggcct cgtcaatatc ggcgcggccg gccgcgcggc ctatcccaaa
    56521 ctgtccctcg agtccgtgct gagcggcaac cccgacgtca tcttcatcgc ccgcggacac
    56581 gacgacgtca tggcgtccgc ggaccgactc ctgaagcgcc tccgctcgct cgacgcggtc
    56641 ctccgggggc gcgtctacac cgtcggcgac ggcattctgc gccccggccc ccgcctcgtg
    56701 gacgccctgg aggagatgtc cgggcacctt caggggcgcg gagcgggtgc cggggcggac
    56761 cggatcaggc ggtccgcccg cggagggcca tgacccggcc gaaatagatg cggtggtagt
    56821 ccttatgggg atagttcccg tcgatggacg gatcgaggaa gtgcccgggg tccatgtcct
    56881 gccagtacat ctttcggcac tcgaccacga gttccgcctc cgcgtaggcg ggcgcggcga
    56941 ccgcggacgc ggggacgggc gtcagacccg acgcggcgat cttgtccagg tcccggcccg
    57001 atttcgtccc gatcagcagc acggcggacc ggtaggcttc cggaaaggcg cacagggtga
    57061 agctttcgta tcgctccatg aaggcgtggg tgtggcgcgt cggacggacg acgacctgaa
    57121 cgaacggcac tttccacatg cagcccatgc tgccccaggc caccgtcatg gcgttatacc
    57181 ggcccgcggc gaaatccccg gcggtgagga cgagccactg cttttcccaa aggtggtgga
    57241 tccgaaccga ccaatcatga acggcaatat cctcaagtgc catggtgtgc ctccctcgca
    57301 aattgtctcc gcccccgtcg agggcgcgac cgcggatccg gtacgccgga aggcgttccc
    57361 gaaccggcgc gttcggtttt tcgtataccg gaatcccctc ctgaaaacaa ccccgggacg
    57421 agtcgccggt gccgcgacac gcggagcttg cattttttta agcgattcgc tataaaaaac
    57481 ccgcttcttc gaccactggg cccacaaccg gtaccggcat gaaatcgagg ctccccatgg
    57541 acaatgcgta ccccctcgaa ccccttttcg gaaagatctt gagcccgttc gaacggtttc
    57601 tccggcagac cacggccggc ggcatcgtgc tcatggggat gacccttctg accctcatcg
    57661 tggccagttc gccgtggggc gacgcctacc ggcacctctg ggagacaccc ctccgggtgg
    57721 ggttaggccc tcagacgctg gagatgtccc tgcatacctg ggtgaacgag ggcctgatgg
    57781 ccctcttctt tttgttggtc gggctggaac tgaaacggga aatcatggtg ggcgaactcg
    57841 cttccctacg caacgccgcc ctgccggtcg cgggggccgt cggcgggatg attgcgccgg
    57901 cgggcatcta tatcctgctc aacccttccg gaccggccgc ggccggctgg ggcattccga
    57961 tggccacgga catcgccttc gccgtgggca ttctggtcct ccttgcctgg cgcatcccga
    58021 gaggggtcat catttttctg accgccctgg ccattgcgga tgatctgggg gcggtgctgg
    58081 tcatcgcgct cttctacacg caggatatcg atatgcatct gctggccgcg gccgccgcat
    58141 tgctgggcat cctggttctc ctgaaccgcg gcgggatccg ccactccctg ccttacgcca
    58201 tcctggggat tctcctgtgg ctggcgctcc tgaagtcggg gatacatgcc accctggccg
    58261 gcgtgctcct ggccttcgcg atccccgcgc ggcccatttt tacgccccat cagttcgata
    58321 agcggctcga tgcgatgaaa caggcccttc acgagaccga gccggattcc gaagggagcg
    58381 acatactccg gagccttgcc cggatgttcg gcctggcaga gcgtctcgaa aggacatccg
    58441 ccgccgtgca gtcgccccag cagcggatgg aacaccgcct cgccccgtgg gtcacgttcg
    58501 gggtcatccc gctgtttgcc atggccaatg cggggctgga cgtggcggcg atgccgatcc
    58561 gggaggtcgt ccttcaaccc gtgacgctgg gggtcgttct ggggctcgtt ttgggtaaat
    58621 tcctcgggat cacgggtttt gcctggctcg ccgtgaagct cgggtgggcg cgcctccccg
    58681 gcggcgtcgg gtggcgtcac atttcgggct cggcctggct cgggggcatc ggattcacga
    58741 tgtccctctt catcgggcaa ttggccttcg cctgggatcc gttgctcttt gaacaggccc
    58801 gtttcggcat catgctggcc tccctgctgt ccgcgattct ggggctcggc tggctctatt
    58861 tcggcgcacg gggggcgcct gccgaggact agcggttgtc cgcctccacc gcctccgtga
    58921 ggcgatcgcc gaaatcggga atggccgcct ccacccggac ccaggcggac atcatgggga
    58981 tgcccaccgg gtcggcgacg aaggctttgg ttgcctcccg cagagcgacg acgaatattt
    59041 ccgtcgcctc gatttcggaa tggcggtcgt agtacaggcc gttgagcagg gacagggcgt
    59101 ggtacttttc gatggtcacc cgggcttcct cgatataggc cgtgtagagg gtccggaaga
    59161 aggcctggct catgacgatg ccgtcctgac tcaggacgcg tatcagggcc tcggcgatgt
    59221 ccttggccat gcgcaccagc cctgcgtcgg gtttcccccg gtccatctcc tggtgcttgt
    59281 gctcgtaggt ttcggagatc tccacctgac agatgcggtt gagggacgcc cgctggtaga
    59341 cttcgctcaa gagcgagact tcgagtcccc aggtgggcga gatgcggatg ccgcgggcga
    59401 ggctgctgac cagggcgaac tcgccggaca gcgcatagcg gaagttgccc aggtattcga
    59461 gaaaggcgtt gtactccagg atccgcttca acgtccggat cagcgggatg tagtagagcc
    59521 gggtcacccg cccgtagagc cggtccgtca cccgggcgta ataccctttg ctgaattcaa
    59581 aatcgaccgc cgggtgcacc acggggtaga tcaggcgcgc gagcatttcc cgggagtagt
    59641 tgacgatgtc cgtgtcgtgg agggcgatgg cgtagaggtt tttgtcggcc agcagatagc
    59701 ccaaggtcat ccagaccgac cgtccttttc cgggagtgtc gatggagaag tcgcaggtcc
    59761 gcaattcctt gtagagtgcc ttcatccgcg gtccatcatg ccagacgacg cggacctcca
    59821 tgggcaggac ggacagggcc ttcttgacct tggagaactg tcgcctgttg gcccggtcga
    59881 gggacaggac caggcgtcgc aggtagcgga cccctttcaa ctcatcgatg atgcggggca
    59941 tggacggggt gtcgaattcc gacacgaggg cggggagcag cagggcgatg ttccgcatgc
    60001 gggcgacggc ctggaggtcg gtttccagtt ccgcgagcgg gcgcttcccg agcctttgca
    60061 gcgtggtgat catgccgtgc tggtagaagt ctgccatggt tcccctcctt ccgtttgttc
    60121 ccagaggtcg agcaggtacc ccttcatgga ctcgaacatc cgggcctgct gttcgaagtg
    60181 ttcctccgca ccagccgcat ctttgtcccg cagttcattc accagcgatg cgactcgtcc
    60241 gtagtacagc gggatcagcg tgtcgaggag cttcatccgt tgcctgggcg tggtgtgaaa
    60301 ggcccgcatg tagcggtaaa cgatgcggac ccaggtttcg acaggcacgg tgaagtcgcc
    60361 ggcgtcggca tgccgggcga gatgcttcac gatgtcgaga tcccgacgct ccaggatctt
    60421 gtgccagacc ccgccgaagt tcctgaagcc gatccggaaa tagtcgacca ggctctcctg
    60481 gtcgatggac agggccggcg gctcctctcc cacatagtcg cccagcgtgg ggacgctggt
    60541 cgaggcctgc acctggcgcc agtggggttc gtagtcctcc ataagctgaa agatggtgcc
    60601 caccacctgg cggaacatgg gccccagatc ggccgccggg tccttttccc cgtggatttt
    60661 ggaccccagc ctggcctggc agatccggta tcccgagacg atcgccgtgg tggtcatcca
    60721 gatgtcgatg ccgaagcggg cgacatccgt ttcccagacc tcgcgctgca gataatcccg
    60781 aaccagcggc agggagatgc cgaaatcgcc cccgatcggc tggcggatcc gctggccgta
    60841 gagggcgcgc gtcaggttgt atgcgatggt gttggtgatg gtcccgtcga gcttgtggcg
    60901 ctgatagtcc ggcacgacga agtcgtaacc gccgaaaacc ggctccagga ggttccggac
    60961 ccagttcggg gtgatggaag ccaggtcgga atcgaacacg gccatcgcct tcggtttgag
    61021 aaaggcggcc acctcgaaca cggcccgcaa tgcggagcct tttcccggca gcccccggta
    61081 gatggtgacg atcttttcga tgttgaagga cttgacgtcg taggaacgcg ccaggtcgcg
    61141 cgtgtcgtcg gtggagcccc cgtcggcgat catgaggagc gccctggcgt cggggtaatg
    61201 gtccttcagg ccctgggcca cggtccggat cacatggacg atggcgggtc cgctctggta
    61261 ggcggggatg ccgatgacga tgtcggcctg tcggagcccc tcgatcttct gacggacgtg
    61321 gtcgcgaatg gctgaggaga atttttgcat cagggtggcc ctccggaaat aaacggtcaa
    61381 tcagggattc ccgccggtct tgccctccgg ccccacaaat ctgaagaccg gcggcgtgta
    61441 ggattccggc agatatgcct ccgggatggt cgtctggttg ccttcccgca gggcggcata
    61501 atggggcgac atgatctcga tgcccgcctc gttgaaggtg tcctggatgt tctggtgtaa
    61561 ttccgaataa atggatgcca tcatggcggg tttgtccgtg taggcgttca gttcgtaact
    61621 gacatagaaa tcatccaggc tcgtctggaa gacgaagggg ggaggcgcct tcaggatgtg
    61681 gaccgtcttg gcggcagcgg ccgtcagaca ttcatgaact ctccgccacg gcacatcgta
    61741 gccgatcgtt acgccggtgt gcaggatgag gccggtcgtt cccgccgccg agctgtaatt
    61801 cacgatgtgg ctgcccagaa tcatggcgtt ggggatggtg atctcctcgt tcttgatggt
    61861 cctcaggcgc gtcacaagca gcgtcttttc gaccacgtcg cccgtggcgt ccgatatccg
    61921 gacacggtcg ccgatccgaa aggcccgcat ataggtcatg atcaggccgg ccacgacgtt
    61981 ggcgacggcc gccgtggagc ccagggacac caggaggccg ataaagatgg agaccccctt
    62041 gaaggccggc gcatccgaac ccggaaggta cggaaacgcg gccaccgccc cgaatgccag
    62101 caggaagaat cggacgatcg cataggtcga ttgggcccac tcgggataga agccgggaaa
    62161 gacgaggacg cccctttcca cggccgagaa gatgaatttg gcgagcttca gaaggtagta
    62221 caggatcagg acgatgatcg tcaggaaaaa caggttcggc agaaaattca ccagggcgtt
    62281 ggcgatgatt gacagcggcg tcaggatgta gcccaggatc ttcgcggaat acgccttcgt
    62341 ccagggaaag aacgtcagga cgaaccccgt atagaagtac aggatcgaca gcaggatgac
    62401 ccaatagatc agcctggctg cccggatcag catgttgccg agctgctggg agcttaaaat
    62461 ctccagcttc tggaatttga tcgtgggaat gcgcacccct ttccacgatt cgatagcggc
    62521 atacaccttg acgaagagtt ttttccaggc gcagacgaac aggcacagaa gggccgtgaa
    62581 cagaacggca aacagcgtgc cggtcaggat cgacttcatg ctcctgtctt ttcggtattc
    62641 cccgatggcg gcctggatcg tcgcggcgtg ttcctgggcg agatcgagtc ttttcttgcc
    62701 cgcggccttg gcatcggcgt ccgtcacggt catgagcacc aaatcgtcga cggaaacgtc
    62761 cgaggaggtc tccccattcg tgaccgagat cttttcgaga ttgagctgat gattatcggc
    62821 cagcttcgcg actcgggcct ggacgaattt cgcccgctct tcgggagaaa gggaaaacac
    62881 cctggcctga atcgaaaaaa gggtgcggtt gtcgaaaacc accggggcgc cgggggcctg
    62941 gacatctttg ccctccttgg gagcggtttg ccgcgcgtcg ccggtgggga cgacgcacag
    63001 cgcggaaatc aggaagatca ggaacgacag ccccagaatg aaacgtcccg tgatgcgttt
    63061 ttgcgaagtc atgctgtgtc tcctgaaggg cctatggcat tccaggcccc gttggcggat
    63121 gacgcggatc ggacatcggc tgcgttcgtc tcgcccggcg tttcccctga gaatcgaact
    63181 ttccggggca ggatagcttc agtgcgtctc tttatcaaat aaaaaatgtg ttcattttcg
    63241 ccgatccgcg ttctgtttac gtcctcggac ggggagcgcg cccgtctcgt cgagcagcgc
    63301 gaggaccgct tcgttccatc cccggctgcc cgggtggttg gcgcgtctgg cgttcgggaa
    63361 gcccccggcg atgccgccgc ctcccgcgtg ggggatgagc acgggaatgt caaccgcgtc
    63421 gagcaggggg gcgtcgtttt cgctgttgcc caggccgatc gtcaatggtg tccggcccag
    63481 gtttctgcga aaaacctttg tggccaggcg gacggcgcgg cccttgtcct gtccgccacc
    63541 cgagaggtgg tggaaccgtc cgccccggat gagactgaac ccacgggtcc gggccatttt
    63601 cctgagggcg gcaacgtgct ccgggttttc gatgatcagg ggttcggaaa actctctggc
    63661 cttggccagg cgggcctccg ccagcggcaa gccggtcagc cgggcgatct cccggggcga
    63721 cagatccccg aatccaagga tatcgtaaca cgccctggcc tcggccacga agcggcggat
    63781 ctcggtatac ggaacgcccc gggagaggac gcaataacgg ccctgcagga ccgcatgagg
    63841 gatcgaaaac cggcggtacc cctcggggaa gaatacggcg gcgccgtttt cgcagatgaa
    63901 ggggtcccgg atgtccatcg cggcctgcag gcgctcgact tccggccgcg ttttgctcgt
    63961 cgtgaagatc agcgggatgc cgtgctcccg gattgccgtc agggcggaat ttgcgtccgt
    64021 ccacgcgtaa tcggcttcgt tcagcagggt gccgtcgaga tccgtgaaaa tgatgagggt
    64081 cggcttacgg ttcatggttc gtgcccgctc gggattgacc gatgcgccaa tccaacgcat
    64141 catacttaac gaatggacaa aaggcgagga aaacgatgat tgctttgcat atcgccaaga
    64201 tatccgtgaa atcttccctt gccttttggg gggcgagggt gcataggaaa aacggccggg
    64261 ggacggccgg gcgggacgcg ggatctctga gcacacgggc gtttcccgcc tcggaatcgg
    64321 gacagggaaa agacagcggg gaaacgtgga taagcgggca cttgcgaagg gagggggcga
    64381 caatgaagaa aacgacgaat agactcttga cgggattcgg catcgtcctg gtcctggtga
    64441 tcctggccct gggcgggtat ctcgtcaaga tgaacgtgga aacgcggaag atgacgccgt
    64501 cggccaccgg ggagatcacg gccggcgtgt atgccgtccg ggattcctac gtcaatcttt
    64561 tcctcatcaa gggcgcggac ggatatatcg ccgtcgatgc gggcaacagc ctggagcggg
    64621 tccggcagga gatgcagacc ctgtccatag acccgtcaca ggtgacggcc gtctttttga
    64681 cgcacaccga tttcgatcat gtggccgcgc tggagctgtt tccccgggcc acgatttacc
    64741 tctcgaaggc cgaggagcag atgatcaacg ggacaacgcc ccgttttgcc gtcatccata
    64801 accggaagct cccccgttac gcactgctgg acgacggtca aaccgtcgat gtttccgggc
    64861 ggagcgtgcg tggcattgct acgcccggcc acacccccgg cgccatgagc tacgtcgtcg
    64921 acggggccta cctatttgtc ggcgatacga tgagcctgaa aaacgggaaa gccgatttat
    64981 ttaatgaatt tttcaacatg gattcgacca cgcaggaagc atccatccgg aagctggccg
    65041 ccctgaaaaa tccgaaattt gtattcaccg cccactatgg attcacggac gatgccgctc
    65101 gggcgttcgg tgatcgatga aggttacccc ccaatggcgc atcggcgccc ctccaccgct
    65161 ggcaaagggg gtgagagacg gtcgggcata aagatcgatt cttgaagatt aaggaaggcg
    65221 ggcgaagccc gcccatgacg ttttgaaggc cgccgtgcag gatttcacag aaggccacat
    65281 tggcaggcaa gtcatcaatt tcagcacgcc gatcatgttc ggcaacctgc tgctgtccgt
    65341 ttacgggatc atcaacatga tctgggtggg gcgactcctg ggccatcagg ccgtggccgc
    65401 catatcggcc tccctgccca tcgtcatgct catgcccgcg tttctgatcg gcctggggat
    65461 ggcgaccaat gttctcatcg cacaggcatt cggccgccgg gacacggcga tgctgaaaaa
    65521 gatcctggcg aattccttta tcgcctcgat gacgttttgc ctgctgatct ccgtgatcgc
    65581 gctcctgttc cggcgtcagt tgctggactg ggtcaatgcg ccgcgggaga ttcaaggcat
    65641 ggcgctgtcc tacctgacca tcatcatggc gacgctggtc tttcagtttt tcatcaactg
    65701 gatgaacggc atgcttcgcg gtttgggaga tgctacaacc gtcgtgaaga tcctcatcct
    65761 gctggcggta ttcaacgtcg tgttcgttcc gctgttcatt cgcggcatcg gacccctgcc
    65821 ccctctcggt gtggccgggg cggcgtgggg cacggcgctg gctgccgtgt cgacctgtgt
    65881 catcggctat ctttatcttc tgcgcttcaa tccctacgtc aatatgcaaa actgggacta
    65941 ccgattggat tggcggatta tccgagaagt tttcgtcatc ggcgttcccg catcgctgca
    66001 gatgatggtc gtctccctgg ctggcgtgat catcatctcc ctggtcaacc gttacggcac
    66061 ggcggtgacc gccgccttcg gcatcggcat gcagatcgat atgctggcca ccatcccctt
    66121 catgtccatc gggatggcgg tgaccacgat cgcaggtcac aatctgggcg cgaggaaact
    66181 ggaccgtgtc ttccagaccc tgcgcgtctc ggtctggttc ggcctgctcg tggcgtttgc
    66241 ctgcacggtc gtgctgctga ttttccctca cgacatcggg gccatcttcc tcaaggaatc
    66301 gctggaaaag gcccgcgtgc tggacacggt ccgcgattat taccggtgga tggccttcat
    66361 tttcccctgc ttcgccgtta tcttcgccat ccagggggtc ttgcgcagtg caggcgacac
    66421 cctggccctg atgggactgt cgttcatcgc catgttcgtc atccgcattc cgctcgccta
    66481 tttcctggcc ggtcccattg gattgaagca ggacggtatc tggatggcga tgctttccag
    66541 cagcgccctg gccgtcttct tgaactggct gtatttcagg tacggccctt ggaagaaacg
    66601 gcggatcctg ggagatcaag gcaaaggctt accggaagaa agggtggaag tccctggccg
    66661 cgcccctatt tccgccagaa accgggcatc aacaagacga tgatcgtgta gatctccagc
    66721 cggcccagca gcatgcaggc gatcaggatc cacttgcccg ccgggggcac gccggaaaaa
    66781 ttgtccatcg gcccgatgct gcccaggccg ggccccacgt tgcccagaca ggccgccgct
    66841 cccgagatcg aggacatcac gtccatgccc agcgccgcga gggccatcgc ggcgaggatg
    66901 aaaatgccga cataaagcat gaagaacccc cagatgctcc gcatgaccgt ctggggaacc
    66961 ggcgtccgcc ccagcttgac caccgtgacg gcgtggggat ggatgatgcg gaggatctcc
    67021 aggtagcagt gcttggccag gagaacgacg cgcaggatct tgattcctcc gcccgtcgat
    67081 ccggccatgc cgcccaggaa catgcaaagc agcaggacct gttgggagag cgccggccac
    67141 gcctcgaaat cggccgtgac gaatcccgtc gtggtcagga gggagaccac cgaaaaggtc
    67201 gcatggcgaa aggcttcgaa aaccgtcgga tagatctttc ccccgtagac gtccaggacg
    67261 acgatcagga tgaaggtccc ggtgatgagc aggtaggcac ggaactccgg gtcccggatg
    67321 aattcccgca gccggccgtg gacgagcttg tagtgaagcg aaaagttgat gcccgcgatg
    67381 aacatgaagg cgatgacgat gcattcgacg taggcactgt gataatggcc gacgctcgcg
    67441 tttttcgttg aaaagccgcc cgtgggcagg gtggcgaagg cgtggcagac ggaatcgaac
    67501 caggacaggc ctccgaaaag caggagcgcg atctgcatga ccgtcaggaa aagatagatt
    67561 ttccagagtg tccgggcggt gtctgagatt cggggggtca gcttgtccac gacgggactg
    67621 ggggtttcag ccttgtagag ctgcatgccg ccgacgccga ggaagggcag gatggcgatc
    67681 gacaggacga tgatgcccat gcctcccagc cattgggtct gcgcacgcca gaagaggatg
    67741 cccctgggca ggcgctcgac atccgtcagg atgcttgcgc cggtggtcgt aaatcctgaa
    67801 atgcattcaa agtaggcgtc ggtaaaatcg ggaatggtcc ccgaaagaag atagggcagg
    67861 gtcgccacga gaccggcgga gatccatccc agggcgacga tggccacgcc gtcccggtga
    67921 ttcaaggcgt agtcttcctg cccccggctc cacaggagag ccccgcctcc gaggccgcag
    67981 gttgcgatca gcgacagaag gaaggcgtgg gcgccgccgt cgccgtagta aagcgcaacc
    68041 agcaggggcg ccgtcatgga caggccgatg tagaagacga ggaccgcgac cagatagagg
    68101 atcaggcgga aattcatcaa aaatactcca gcttgacggt caggagttct tcgatcttct
    68161 tcgagacccg ttgcagggcg aaaatgatga gccggtcgtg gggccggatg atggtgtcac
    68221 cgctgggaat gatgatttcg tttccgcgga cgatggcccc cagaatcgcc cccgggggga
    68281 agggaacctt ggacagcggg gtgttgacga tatccgacgt ttcgagcgcc tccgcttcga
    68341 tggcttcggc ctcctcttcc cgaagggggg cgacggagat gatgcgaccc cggcggatgt
    68401 actggagaat cgcgcggacc gccgccatgc ggggattgat gaccgtgtcc agaccgatgg
    68461 ccgacacgat gtgcatgtag ctcagcttgc tgacccgggt gatggtccgc cgggtcccca
    68521 gttccttggc gagaagggag atgaggacgt tgctctcctc gtcgccggtc agggcgatga
    68581 gcagatccat gtcgccggcg tattcctccc ggagcaggtc ccggtcggcg ccgtctcccc
    68641 ggatcacgat cgcccggtcc agaacggtgg ccagcttttt gcacttctcc ccgtcctggt
    68701 cgatgatctt gacgttcatg cgacgccggt cgagggcctt ggcgacggcc gagccggttt
    68761 cccccgcccc gacgatgagg acgtttttca gggggcgggt cgaaatccca agccggtcga
    68821 gcgtagggtt gacctccgcc cgtttcatca ccagatagac gagatccatg ggctgaatgg
    68881 tatcgcggcc gctggggatc aggatcttgt ggccccggac gatggcgccg acgagcaggt
    68941 tgccctcgta cgccttcagg tcggtcagcc gcttcccggc caggggggac tcggtggtga
    69001 tgaggaagct gaccagcttg acctcgccac ccacgaagtc gatcacctcc gaggcgcccg
    69061 gaacctccat gaggccgaga atcgtgtcca ccatcatggc gttcgtattg atgatcaggt
    69121 ccaccccgag gagatcccgg ctgaaaaggg tttcttcccc gagatactcc cggttcttga
    69181 cccgcgcgac cttgaccatg tgcgggttga gactgcgggc cagcaggcag gcaatcaggt
    69241 tgacctcgtc gctgtcggtg gctgccgcca gaagctccgc ttcctgaatc ccggcggcgc
    69301 gaagcgtctg gggactcgta ccggagccca gaaatgcctg cacatcgaga ttctcgctga
    69361 tccggcggat tttggcggga tcctggtcga tcaggacgac ctcctgactt tcatcggaga
    69421 gcttcttggc gatatggtac ccgacttctc ccgcgccgat gatgatcact cgcatgattt
    69481 cctcccgcaa tcgggtgaac cgaggactct ctatacaaca aacggatcgt ggatgacagc
    69541 aggaccctca cgccgggaat cgccctcatc gttcctccga tgtcccgggg cggattccgc
    69601 cgctgcagga aggcgtgcgg ccgggcgagg cgtttccgca acgccggacc ctcccgccgt
    69661 gtgggatggt tcatcgaata ccaccgcggc cccgctttga caagcggaaa gccttccccg
    69721 ggatgatgga ttagtgggta caagatccgt caggaccttt gatacggcgc cgatgacccg
    69781 agagcgatat ttcccttgat cttgcttagg cagtgtggaa tgaaagggct cattcacttt
    69841 cctggaggag gtcacgaaaa tcccatgtca ggagcattga tcagcgagca gatgcgcgcg
    69901 gccgtcgggt cgccgctggc gtccggacaa ccgtttgcgg tcgaaaaagg gtcgatccgg
    69961 gatctggcgg aggccatcaa ccatctccac cccctttacg tggatgaggc gtacgcccaa
    70021 agcacgggtc acggcagcgt gctggcgccg ccgacgtacc cgtcctacaa cctggcgctg
    70081 ggcgcgcccc tggaggtgct cacgttcgag ttccccgtgg gcagtatcct gcacggcagc
    70141 gacgactggg aattcctcgg aaacatccat gcgggcgaca ttctcacgcc gcgggggaaa
    70201 ctcctggacc tctacgagaa acagggcaag cggggcggca tgctcttcat cgtttcggag
    70261 atcacctaca cgaaccagcg gggcgaggtc gtggcgatct accgtcccac ggaagtcgtg
    70321 atcgccctgc cgacggctta aggagcacac aggtcatgaa gacgcaacct tactttgaag
    70381 acatccgcgt gggcgaggaa atcccgcatc aggtgcggga accgctctcc acccgcggcc
    70441 tggtgaagtg ggccgcggcg gtccgggact tctacgaggt ccactacgac aaggattttg
    70501 cacgcagcat cggcctgcgg gacgtcatcg cccacggccc caacaagtgc gcgctcctcg
    70561 gccgcctgat gacggaatgg atcggcgagg ccggacggct gcacaggctc tcctgtacgc
    70621 acaaggcgag caatttcccc ggcgagaccc tggtctgccg cgggcgggtc aaggacaagt
    70681 attccaaaga cggccggcat tacgtggaat gcgaggtctg ggtggagaat caggacggcg
    70741 tcgtggccgc cccgggtgcg gcgacggtgt cgcttccttc gcggggggta gaaccgctcc
    70801 cgtcggcgcg accgtgaatc cgcgcttgac aaacaccccc gatcttttct aaggtcggtc
    70861 cgtctcaaat ggatggatgt gaggaagagg ggtgagaagc ccccgctgcc ccgcagccgt
    70921 gaagggaacg aacgcccagg atgccactgt cgccggtcaa cggcgatggg aaggcgggtc
    70981 agtaggtcgc ccgaagccgg aagaccggtc catccgcaag tcctcgaggg aggcctccgc
    71041 gtgcgaccga ccgtccatcc cattgtcact ccgttccggg ttctccgtat cagcggtatt
    71101 ctgtttctga tctgtctggc cgttttcgtc gggtcgctct tcgccggtcc ggggcaggtc
    71161 ggcgtgcgca ccctgctggc gtcgctcctc tcgacgggct ccggatcgtt tgccggcgac
    71221 gccgatgcgg ggcagaccat ccttctgtcc ctccggcttc cccgtcttgt cttcgcgggg
    71281 atcgtcgggg ccgccctgtc cgtgtcgggg gtcgtgtttc aggcgctgtt gcgcaacccg
    71341 ctcgccgacc cctatatcct cggcatctcg gggggcgccg ccgtgggggc gatcgccggg
    71401 atcctgctcg gcgcggcggc cgttcctttc ggcgtccccg gcctcgcctt cgtcggggcg
    71461 ctgacgaccc tgctggcggt ctttgccgtg gccggcgccg gaagggacct ccgttcccac
    71521 accctgcttc tggccggggt catcgtcaac gccttcttca acgccgtcat catgtttctc
    71581 ctcgccgtca gcagttcggg cgacctccac agcatcatgt tctggctcat gggggatctg
    71641 agcctcgccc ggggacggga catcctgtcc gtcggagcgg cgctcgtcct gggcttctcc
    71701 gtgatctgcc tgtacgcgcg tccgctcaac ctgatcgtga cgggggagga gacggcccgc
    71761 accctgggcg tcgacccggc gacggcgaag cgggtccttc tcgtcgtgac ctcgctcatg
    71821 accggcctgg ccgtttcggt gagcggcacg atcggtttcg tgggactgat ggtgccgcac
    71881 ctgatgcgga tggcctttgg cccggatcac cggctgctgc tgcccgcctc cctctttttc
    71941 ggggcggcct ttctcatcct ggccgacacg ctcgcccgga cgctgcttgc cccgatggag
    72001 ctgcccgtgg gcgtggtcac ggccctgtgc ggggcgccct atttcatcta tcttctgagg
    72061 aggatgaacg attaacgccg ttctggacat cgaccgggtc cgtttccgct acgccgaaga
    72121 ctgggtgctg acggacgtcg ccttttccgt ggccgccggc gcctttctgg ggatcatcgg
    72181 tcccaacggt tccgggaaga cgaccctgct caaggtgatg aacgggctcc tccgccccca
    72241 ggcgggcgcg gtccagctga acggccgtcc cctgagcggg atgcggcgcc gcgaggtggc
    72301 gcgggtgatg gccgtcgtcc cccaggacgc ggcgctgctg ttcaccttca ccgtggagga
    72361 gatggtcctg atgggccgat cgccgcacct gggccgtttc gggttcgagg ggaaggggga
    72421 cttccggatc gcccgcgcgg ccatggaccg gaccggcgtc ctcgccctgg cgcaccggcc
    72481 ctatgacgcc ctgagcggag gggaaaggca gcgggtcctg atcgcccgcg ccctggcgca
    72541 ggaaccgcga attctcctcc ttgacgagcc gaccgcctat ctcgacatcc gccatcaggc
    72601 ggggatcttc gacctgatga aggacctgaa ccgccgccgg ggcctgacgg tcgtcgccgt
    72661 cacgcacgac atcaacctgg cctcgctcta cagcgacgag gtcctgctcc tggccgacgg
    72721 gcgcgtcttt ggcgccggcc cgccggaggc ggttgtcacc acggaaaaca tccgcggggc
    72781 gtacggcatc agcgtcctcg tggaccccca cccggcgaca ggcctgcccc gggtcacgcc
    72841 ggccggctat gccgggggcg gagggcccga tccggcaccc tgaaaggccc gatcctttct
    72901 ccgtgccgga cccgtttcga tccgttgata ccgcgtccgc acatccggac ccccgtcttc
    72961 gggagcggtc gggccgcggg gcaagcggac cgaaggatcg ccgcccggtg cccggattca
    73021 tccggttttc cgggtacgaa atgatttcgt gaagttgggc gagtgggcgc cgcacgcgcg
    73081 cgcactcgat tcttgaacgt cccgcggcag ggaggaagcc ggtgcgaatc cggcgctgcc
    73141 ccgcaactgt aagtggaacg acctccgcac gaggccacgg gttcgcggtt gaacccggaa
    73201 ggcgcggaca gtaggacgcc acgagccagg agacaccgcc gcaggccagc gactcgattc
    73261 ccgcggggga aaggagaaac gttccatgag gatccggtta cagtggcttg tgatgttgtt
    73321 ttgtttgtcg ggggcgatgc tcacccctgc ccccggttta tctgccgccg cgacggccga
    73381 acctccgggg gcggcgatta cgctcgagga ggtggtggtg accgccacga agaccccggc
    73441 gaagcggagg gatgttccca atgcggtcct cgtccttgac gccggggaga ttcgggcctc
    73501 cggcgcgaaa acgatcgggg aattgctggc caacgagccc gggatcgact ggcagaccta
    73561 cgggaattac ggtggcgccg cccaggagat ccacatccgc ggcatgcggg gaaacggcac
    73621 gcaggtcctg atcaacggcg ttcccgtcaa ctccccctct ctgggtgttg cggatgtggg
    73681 caggcttccc atcgacgccg tcgagcgcat tgaaatcgtc aagggatccg ggtccctcct
    73741 ctacgggtcg ggcgccatgg gcggcaccgt ccatatcctg acgaaggggc ccctaagaga
    73801 caaaacggac ctcaaaatcg gcgccggcta cggttcccgg aacacctggc gggtggcggc
    73861 ggaaagcggg atgttcgtcg ccggggattt cggatattac ctgaccgccg gcagaacgga
    73921 aaccgacggc ttccgggaca acagcgatct ccggcagacc gatgcgtcgc tcacgctggt
    73981 tctggacagg aaggacggga tgaatgtccg tctctacgga gattacctgt accggaaata
    74041 cgggatgccc ggtgtcgaac cgcccgccgg cacggggacc tacgccatcg gaacggagac
    74101 gttctacaac cgtgacgcgg ccaccctgct ggatcgtagc ggcgacaagg acggccgcat
    74161 cgtgctcgag gtcaagggga agcccgcccc atggctgggc tatgatttca agggatacta
    74221 cacggacatg gagaatcaca atgttcagcg gtacgccttc aacggatcgg gggacgaaaa
    74281 ctgggtgacc aaccgggtgc taggcgcgga cggacatgtg gacgtttacc ccttcgaggg
    74341 cgccaaactc cttctgggcg gcgagtacaa gaacttcatc tggaaaaatg acaattttga
    74401 cctggacacc tccggccgtc gaaccgtcga aacggcccat gatgccggtc tccatacgaa
    74461 gggcgtcttc acggaggcgg aataccggcc gtcgcggtac tggaagatgc tggctggttt
    74521 ccggcacgaa aatcactcgg ccttcggctc gggaaacctg cccctcttcg gcctgatcgt
    74581 caatctcggc gaggcgacgg ccctgaagat caaccatggg aaacatttcc tggcccccac
    74641 ccccaacgac ctgtactggc cggccgatcc gtatacccgg ggcaacgtcg acctgaagcc
    74701 cgaaacgggc tggcataccg atgtgacggt agaacagtcc ctgctgaacg acaaggtgtt
    74761 cgtaagcctg tcgtatttcc attggaaggt ggaggacaag atccagtggg aaccggattc
    74821 gcggggtgtc tggagcccca tgaatctggc ggactatcgg gcggagggcc tcgaggcggg
    74881 ggtcaggatc ggaccctttg atggcttgac gctggccctg ggctacacct atctggatgc
    74941 ggaagaggaa aaccgcgcct atacgagaca ggactatgga tggccgccgt tcatcgcccc
    75001 ggatttccgg tacgccatgg tcccccggcg tgccgccatg acgccggagc atcagttcaa
    75061 gggcgacctc acgtaccgga gccgcttcgg actgaccgcg acggccacgg tccgttacgt
    75121 gagcgaccgg ctcgtttaca atacggagac caccacctat cccgacaccg aaaccgttgt
    75181 ccataccctc cgttcctact ggacggcgga tctgaaactc gaacagcgtc tgggcgggca
    75241 ctgggtcctg acactggcgg gccgtaacct gttcgaccgg gattacgaca cgcgtctggg
    75301 atcctttacc gatcaaacca ccctcaagag cgcgctgtgc ggatatcccg gggcgggacg
    75361 gtctgttttc gcgggcgtgg cttacgagtt ttgacgacgc cttgcgtgta tggcacgcgg
    75421 tgcctgttcg atccgccggg tgaagccggt ggccccgccg cgggggatgc cggccccctc
    75481 cggctatgcg tcgatcgcag cgagcatcgt ccggaggtcc gtcgtcggct tcaggcaggc
    75541 gttgccggtg cagacgtagg ccgtcgcccg cccgtctatg catggcatgt ctcgcgtgta
    75601 gggggcaagg cggcagatgg ggggagaggc ctcgccggcc gggcgcagga tgacgaccgt
    75661 ccgcgggaga aaacgggacc tcaaggcccg cagcagggcg cgggtgtctt ccgcggccgg
    75721 gtcgcccacg accaccactt cccgcgccgg tccgaaggcg aaatcgaggg cgaccatgaa
    75781 ttgggcatag gcccccggcg attgccgcag atcgcgatcg aaggccttgg cggcatccca
    75841 cgcctcgtcc agcagcgtcg tttctcccgt catgcgtccc agccgcagca ggttccagac
    75901 ggccacggcg ttgccggacg gcatggcccc ttcgaagatc tccttctggc ggacgaggat
    75961 ctcttcccca tcccgcggcg tgaagaagaa gcccccacgg ggatccttga aggcggtcat
    76021 caggacgcac gtcagggcca gggcctcctc cagataggac gactccccgt tggcttcata
    76081 gagttcgatc agggcaaggg tgaagaacgc gtaatcgtcg agggtgcccg caatgcccgc
    76141 ctcgccgtcg cggaagcggt gcaggagggc gccgtcgggg cggcgcatcc gaccgaggat
    76201 gaaggccgcg gccctttggg cggcgtcgat ataggccggt tcgtccaggc aggccccggc
    76261 cttggccagg gacgcgatca tcaggccgtt ccagtcggtc aggatcttgt cgtctttctg
    76321 cggcggaacc cgccgctgcc ggacggcgaa aagcgtctga cgggcccgct cgacgcgtgc
    76381 ttcgctctcc gggtcgatga tcggcgaggt cccgcccgcc ccttcggttg cgggcgcaag
    76441 atgcgggatg ttcatgcccg ttttgcgtcc cgtgagctcg tcggtgaagt tgccggtttt
    76501 ctcgaagcgg aaggtgcgcc tgaacagatc ggcgtcggcc tcgtccagga tgccgtcgac
    76561 gtcttcccat cgccagaggt agaatttccc ctcttccccc tcgctgtcgg cgtcctcggc
    76621 ggaataaaag gctccctcgg gcgacgtcat ccgggtcagg acataggaga agacctcccg
    76681 cgccgtgtcg gcatacgttt tctcgcccgt tgcctggaag gcttccgtat aggcgtaggc
    76741 cagcatggcc tgatcgtaga gcattttttc gaaatggggg acgagccagt gcgggtccac
    76801 gctatagcgg tgaaagccga agccgatctg gtcgtagatc ccgccgcggc gcatggcctg
    76861 gagggtcgcc tccgccattc ccagggcgta ccggttgccg gtccgtttcc agtaacgcag
    76921 gaggaacagg aggttctgcg gggtcgggaa tttgggccgg gagccgaagc cgccgttcgt
    76981 cccgtcgaag agacgggcca gttggtcaaa ggcggcatcg aggctttcgg ttcgcggcgc
    77041 ggccccgggc ggggcctcgc ggccggcccg caacgccgcg acgacctcct ccgccttatc
    77101 gacaatgtcc tgccgccggt tccgccagat ctcgtcgatc cgggggagga gggccagcat
    77161 gccgatctgt ccgaaccggt cctgccgggg gatgtacgtc gccgcgtaaa agggcctttt
    77221 gtcggcggtc atgatgatcg tcagcggcca gccgccgcgt cccgtcatca tctggcaggc
    77281 ggccatgtag atcccgtcga tatcgggccg ctcctcccgg tcgaccttga tgttgatgaa
    77341 atgccggttc atcagggcgg ccacggcggg gtcttcgaac gattcatggg ccatgacgtg
    77401 gcaccagtgg caggtggaat agccgatgga cagaaagatc ggcttgtcct gccgggccgc
    77461 ctcggcgaag gcctcctcgc cccacgggcg ccagtcgacg gggttcgtcg catgctggag
    77521 gaggtagggg cttttctcgc cgctcaggcg gttcgggggg gggcaggcgg ccgctcgggg
    77581 gtcgcccatt ccagatgaag gcattgtcat tttccccgtt ctcgtctaca gtgtgcgcat
    77641 catcataccg ttttttatcc ggatggaaag attattttgg acaggggagg gcgggatacg
    77701 atgatcgaaa aaatgacgtc tctggtccgg gaaaaggaca cctgcgtcct cgccacggcg
    77761 gcggggggcg agccgcactg ctccctcatg tcctatgtgg cggacgacga cggtctttcc
    77821 ctttatatgc tcagccgcaa ggggacaaga aaacacggca acctgatgaa caacgcgcgg
    77881 gtcagcctgc tcatcgacac gcgggaagac attgccggcc gggaccgcgg ggccgtcagg
    77941 gccctgaccg tgcggggcgt tttccagtcg atcgacgatg actcccgacg gggcgccatc
    78001 ctggaaaaac tgctcgatcg tcatcccccc ctccgcgaac tggcgggtca tccggatatc
    78061 gacgtctttg aagtgaaagc ggtttccttc gaactgctgg aaggcgtctc ccgggcgacc
    78121 gttgtcgagg tcgcctgacc gccggcccgc gcggcacgtc ggtcggcttg gcccatcccc
    78181 cggccggggg atgggaatgc gctgccggtc cgggaggatc gttcggaacg gtcggccccg
    78241 ggttccgccg ggcgcgggcc cggggcggcg gcggtccctc tgcgggggaa ggcgcccggt
    78301 tcgggaccct cggcgggcgg ccccgcgaac gcctgggcga tgcgcatcca gtgccgggcc
    78361 ccatccccgg cgacgttcag gtctgtatcg tcgacgtgcc gccgctgtgt caccacgagg
    78421 caaaaatctt ccgccgctcc ccggatcgct tcgccggcgt ccgccgctcc ccaggtccaa
    78481 acctctccgg acggtcccag gagttccacg cggacggggg tcgcgggcgg gggcaggccg
    78541 cggatcagga agctccagtc acgagtgctc accccgagat gggcgatgtg ccggaggcgg
    78601 atcgtcgccg ggcgctcctg gcccagggcg tccaagatgt cctgcccgtg cgcccaggtt
    78661 tccatgagcc tggccgtggt gaacgatcgg acgcccatgg gcggtccgaa ccagggaagg
    78721 cggtgtcccg gcgggagggc cttcaggccg tcgatcagac cggaccgctc tcggcgccac
    78781 cagtccagaa gcgcttccgt ggacagcgcc cggccgatct tcagcgtctc ctcgaaggga
    78841 tccccgtccc gggaggcctg atccatctgc gcggcgaacg cggccgcgtc cgtcgcggac
    78901 agccgcgccg cggcgtcgaa gaaggccagg tggcagatct cgtcgcggat ggtccagccg
    78961 taaaaggggg tgggccggtc ccagaccgct tccgcgagcg ccgtgacgac ggcctccagc
    79021 gtctcctgct ccgccgcgag atcgccgatg aggtccgtca tctccgcctg cctttcgtcc
    79081 gccgcggggg cctgtgcgcc cgctgggtgg aacgcgccga aaaactcatt ttcccttgag
    79141 ttctttcagg atcaggtccg cgcagagcat gccgctctgg gcggcgctgt taatggcgag
    79201 gccccggccc cggtaggtgt cgccggcgaa gtagaggttg gggatccccg gcgccttgag
    79261 ccccggtttg aaattcccca cgaggcccgg ctggcgggcc agaccgtcgc agccgtccac
    79321 gtaatagggc agacgccatt ccgtcttcgc ttcgatcccc gggaacatgt ctttcagatc
    79381 ttcccagtgg cgcttgagga gccgttgacg catgaacttg tccgaggcct ccgcgtgctc
    79441 gcagaccagg tccgtgtgga taacctgctt tcccgccggg gccacgtgct cggagaaatt
    79501 cgaggccggg aaggcctgga agggaaaacc ggcgtgccgg gttttcaggg cgctgaagaa
    79561 gcatttcttc cggggatcca ccaccggctc cttcaggccg atcatgaagc cgatcagccc
    79621 cgtgatctca tgctggatgt cggtgatgcg cttgacccac cagcgcggca gggggctcgt
    79681 gacggggttg aaatccagga tctgggacag cttgtagatg ggcagggcgc acaccacccg
    79741 ctcggcggtc aacagctccg tttcgaagat ccggtattcc tcctggtaga tgtgcttctg
    79801 cgcggggacc cgcacgcccg cggcccggcc gtccgcaacg cccacctcct ggacgtcgca
    79861 gttgaggcgg atctcgccgc ccccctcccg gatgacctcg gccagtccgc gggtgacccc
    79921 ctccatgccg tcccgcgggt agcttgcctg cagggcctgg cgggtcttca ggaggttctc
    79981 ccgcgcgatg tagaggacct cgcccgcggc catgttgatg gcgtgcggga tggtcgtcat
    80041 gatcatcccg agatcggtga agagttcccg aaccggggcc tggggaacgt tttcatggag
    80101 ccattcggca agcgaccggt cgtcccacgc ctcgatctcg gcgtcgctca gcatggcgat
    80161 cttctgcatc agatcacgga aggcggcctt gtcctcccgg gtcatgggga cgagctccgc
    80221 cacgctgacg aacttttccc cgttccagat gtccacggtg tcgctgaagg gcgcccaagc
    80281 caccttcttt cccacccgct cgttcagggc gttacaggtg gacttctccc cgagctcgat
    80341 catgtgcagg ccgatctcga cccgccagcc cgcggacgga tagccgtcat agctcatcat
    80401 tctcccgccg aacttgggaa aacggtcgag aacgagaacc ttctgcccgg cctccctggt
    80461 caggatcgcc ccaaccccca ggccggcgat ccccgccccg acgacaatcg tatcgtacgg
    80521 cttggtcatg attctcctcc ttcacgtggt ttgctgcgac ggcttgtccg gccggtggcg
    80581 ttcgatccac gtcctgacga gtgcctccga aggacgcacc gtaacttacc agatgtcgag
    80641 ggccttcctc aagcaaaacc ttcccgggca gcgaaaaaca tcccgccgct cgcggcaggc
    80701 gggtcttccg ccccggtgtc ggagggaaca aggcccgggc gcgggacgca aaattgcctt
    80761 gcaaaatgac ggactccgtg caaaattcac ggcgcatgcc gttgccggtc ggcggatccg
    80821 ttgctggagc ctgatgcggc ggcatcgcgt ttttcaaaga ccccaaggaa cgcggacgcg
    80881 tttttgactt catcgagagg atcaccgaaa tgaaaatcaa gttcatggga gcggcgcgga
    80941 cggtgaccgg ttcgtgcttc atcgtcgagg cggcgggtca ccgcttcgcc gtcgactgcg
    81001 gcatgcatca gggcaacgcc gagatcgacc agcgcaactg ggaggtggac ctctacgatc
    81061 cggctgggat cgagttcttc atcatcacgc acgcccacat cgatcactcg gggctgattc
    81121 cccggatggt gcagaagggg tttcacgggc cgatctacat gacgccgccg acaaaggatc
    81181 tccttcaggt gatgctcctg gacagcgccc acatccagga aacggaggcc cagtggaaga
    81241 cgaagaggcg gctcaggcac ggggacaagc gcatccagcc cctctatacc cagaaggacg
    81301 cccaagcgtc ttttcccctg ttcgagtccg ttccttacga gaaggtgttc gacccctgcg
    81361 acggcgtgag ggtcaacctc cgcgacgccg gccacatcct gggggcggcc ctggcggaac
    81421 tccggatccg ggataacggc gccgacacga agctcgtctt ttccggcgac ctcgggcgtc
    81481 cgtcccagct catgatgaag gaccccagcg tcgtggagga ggcggacatc ctcttcatgg
    81541 aatccacgta cgggaaccgg aaccacaaga acgaggacgc aagcctggac gaactcgccc
    81601 aagccgtggc ctacagccat gaacgggggg agaaggtgat catcccggcc ttcgccgtgg
    81661 agcggacgca ggaaatgatc tattccctgc acctcctgga aaagagcggc cggttgccca
    81721 aggacatgcc cgtattcgtt gacagcccac ttgccattgc ggcgacggag atcttccggc
    81781 gccacaaaca tcaattcgat gcggaggccc aggcgctgct cgcccgaggc gaggatccgc
    81841 tgacgctgcc cggcctgcgc tatacccaga gcacccagga ctcgatcgac atcaacgccg
    81901 ttgccggtcc cgcggtcgtc atctccgcga gcggtatggc caatgcgggc cggatcaagc
    81961 atcacctgcg tcacaacctc tggcgggagg gggcgagcat cgtgttcgtc ggcttccagg
    82021 cggcgggaac gacggggcgc cggatcatcg acggggcgga gaaggtgcgg ctcttcggcg
    82081 aggacgtcgc cgtcaaggcg aaggtcttca cgatcaacgg tttttccgcc cacgccggcc
    82141 agacgcaact gctcgactgg ttcgcccact tccggaaccc ggccatgcaa ctttttctcg
    82201 tccacggaga atacacggcc cagcaggcgt tggcgggtct catcgaggag cgcttcggga
    82261 tcaaggccgt catcccggat tatctggaag agacgaccct caaccgtggc gaggcgctta
    82321 agcgcatgga attccccgag caggccgcgc cccggatcga ctgggagtac ctcatcgccg
    82381 acgtggaggc gaaactggcc catctgaaag aacggcaggc gaacatcgaa ggcaaggccc
    82441 gagtggaaca gacggacctc cgggatcgtt tgctcgaagt gaaccggagc ctgatgcagc
    82501 tcatctcgga gagctgaccg tcccggacag ggggacaccc gatggcagaa gacctttccc
    82561 ggctgaagat cgacaagtcg gcgcaggccc tacggccggc gaggcggcgc gcatttctcc
    82621 tgccggtcct gatcctgctc gttgcggtca tcctcgccgc gctttacgtc ctcggcgtcc
    82681 tgaagccggc gtccgccgtg cagacggcga cggtctcttt cgtctacccc tcccagaatt
    82741 tcacggtgct caacgccagc ggatacattg tcgcccaacg caaagcggca gtcgcgtcga
    82801 aggccaccgg ccggctgacg tccctctggg tggaggaggg gagccgggtg acccgggggc
    82861 gggtcatcgc ccaactggaa agcgacgatg cggaggccag ccgggatcaa gcggcggcga
    82921 acctatccgt gagccgggcc agcctgaatc aggcgaagac ggtgctggag gatgcggcgc
    82981 gggattacga ccgcaaccgg cggctgctcg aaaagggcat cgttgcccgc aatgccttcg
    83041 atctggccga aacccgatac cggagcgccc aggcggccgt gaccggcgcc gaagcagcga
    83101 tccgagccgc cgcggccggg ctgcagggtg cgaacgtcgc cctggaatac acccggattc
    83161 gcgcgccctt cgacgccgtg gtcctcacga agaacgccga catcggagat atcgtcaccc
    83221 cgctgggggc ggcggccaac gccaaggcgg ccgtggtgac gattgcggat ctcgggtcgc
    83281 tggtcgtgga ggtggacgtc tcggaggcga acatcggacg ggtgaccgtt gggcagccct
    83341 gcgagattca gctggacgcg attccggacg agcggttccc cgggagggtc catatgatcg
    83401 tgcccaccgt ggaccggagc aaggcggcgg tcatggtcaa ggtccggttc ctgagcgcgg
    83461 acccacgcag gctcccggac atgagcgcca aggtcgcctt cctgacccgg ccggtggggc
    83521 cggacgaggg gaagccgcgg ctgggcgtgc cccgcgcggc cgtcgtgaag cggggaggtc
    83581 ggcaggtcgt ctacgtcgtt caaggcggcc gcgtccggga aacggacatc cggacgggcc
    83641 gcgggctcgg ggagatgatc gaggtggcgg gcgggccgaa ggcgggcgag aaggtggtcg
    83701 tgaatccgcc ggccgcgctc ggggacggcg ggcggatcga ggtggcggaa ccgtgacgac
    83761 ggaagcgccg gcacccatca tcgacgtgca gggggtcagc aagtcctacc ggcgggggga
    83821 gctggtggtg ccggttctgc acgacatcac cttcaccatc gcccccggcg agttcctatc
    83881 cctcatgggg ccgtcgggct cgggcaagag cacgctcttg aacctcgtcg ccggcatcga
    83941 ccacgtcgac ggcggccgca tcattgtcga aggcgtggac atcacccggc tcaccgaaac
    84001 cgaactggcc cggtggcggg cgaattccgt cgggttcatc ttccagttct acaacctgat
    84061 ccccgtgctg accgccctgg aaaacgtgga gctgcccctg cacctgtcgg ggctttcccg
    84121 gcgggcgcgc cgggagcacg ccctgacggc cctggacctc gtcggcctgt cccaccgcct
    84181 cgatcatttt cccggccagc tctccggcgg ggagcagcag cgcgtcgcca tcgcccgggc
    84241 ggtcgtcacc gacccgacgg tgctcgtggc ggacgagccg acgggtgacc tcgaccgggt
    84301 ttcggccggg gaggtcctgg acatgatggc acgcctgaac cgggagctgg gcaagacgat
    84361 cgtcatggtg acccacgacc cgcgggcggc cgcacgcgcg catctgaagc gccacctgga
    84421 caagggagtc ctgagcgccg atgatccttc tgagaatcct gattagaaac gccttccgga
    84481 accgcctgcg gacggggctg acgatcctcg ggatcaccat cgcgatcctg gccttcggcc
    84541 ttctgcgcac ggtcgtgagc gcctggtacg cgggtgtggc cgcctcctcc gccacccgtc
    84601 tcgtgacgcg caacgccgtc tccctcatct ttcccctgcc gctggcctac aaggacaaga
    84661 tccgccaggt cgacggtgtt tcccacgtgt cctggggaaa ctggttcggg gggatctaca
    84721 tcagcgagaa gaacttcttc gccaatttct gcgtggaccc tcgtagttac ctggccctgt
    84781 atcccgaata catcctgtcc gatcgggaca ggaaggcctt cctgtccgac cgcaaggggt
    84841 tcatcgcggg ccgcaagctc gccgaccggt tcggctggaa gccaggcgac acggtgacgc
    84901 tcaagggcgt catctttccc ggcaactggg atttcgtcct gcggggggtc taccgcggcc
    84961 gagaccggac catcgacgag acccagtttc tcttccagtg ggactacctc aacgaaacgg
    85021 tgaggcagcg ctaccccagc cgcgccgacc aggttggctt ctacatgatc ggggtggcga
    85081 gccccgagcg ggcggcggat gtcgcggggt ccatcgaccg gctcttcaag aattccctgg
    85141 ccgagacgct gactgagacg gaaaaggcct tccagctcgg cttcatcgcc atgacggagg
    85201 cgatcgtcat ggccattcag ctcgtctcca tcgtgatcat cgtcattatc atggcggtgg
    85261 tcgcgaacac gatggccatg acgacgcggg agcggatcgg ggattacgcc atcctgaaga
    85321 ccctggggtt cggcggccgg cagatcgccc tcctcatctt cggcgaggcc ctggtcatca
    85381 ccgggatcgg cgccgccatc gggatggccc tgacctatcc ggcggcccgg gccttcggca
    85441 gcacactgtc caacttcttt cccgtcttca acgtcgcgcg cgagaccctc tggctcgatc
    85501 tcggagcggc gctcgtcgtg gccgtcgcgg cggcggtgat tcccacgcag cgggcgatcg
    85561 ggatccgtat cgccgacggc ctgcggagga tcggctgatg gcggtgcctt tctcctacag
    85621 cctccgcaac ctctggaccc ggcgcatgac cacgatcctg acggtcgccg gcatggccct
    85681 cgtggtgttc gtcttcgcca cgatcctgat gctcgccgaa ggtctcgaac ggaccctcgt
    85741 cgagacggga tcggcggaca atgtcgtcgt gatccgcaag gggtcggcct ccgaggtcat
    85801 gagcggcgtg gagcgcgcgc aggcggcggt cgtcgaggtc cagccggagg tcgccgtcgg
    85861 ccgcgacggc cggagactgc tcgccaagga actcgtcgtg ctcatcgccc tgccgaaacg
    85921 ggggaacgga aaggcgtcaa acgtcgtcct gaggggggtc ggccccgcgt ccctggagct
    85981 gcgtccccag gtccgcctgg tggcgggacg gctgccgcga atggggtcct cggaggtgat
    86041 ggccgggcgg agcgtcgtga agggttttca gggcgttggg ctgaacgagc gggtctgctg
    86101 cggcctgcgc gactggcggg tcgtgggggt cttcgatgcg gggaacaccg gcttcagctc
    86161 ggagatctgg ggggacgcgg accagttcat gcaggccttc cgccggcccg cctactcctc
    86221 cgtcctcttc aagcttcggg atgcccggga atttccggcc gtcaaggcgc gcctggagac
    86281 ggacccccgc ctgcccctcg aggcgaagcg ggagacgcgc tactacctgg accagtcgga
    86341 gatcatggcc aagttcctcc gcatcctcgg cgtctccctg acggtcattt tctcgatcgg
    86401 cgccatgctc ggggccatga tcaccatgta ctcggccgtc gccaaccgga ccgccgagat
    86461 cgggacgctg cgcgccctgg gcttccgtcg ccggaacatc ctgatcgcct tcctgatgga
    86521 gtcgctgctg ctgggcttca tcggcggggc ggtcggcatc gcctgctcgt ccctgaccca
    86581 actgctgacg atctcgacgc tgaacttcca gaccttctcc gaactggcct tccggttcac
    86641 cctgagcccg gcgatcgccg cccaggcgat gggtttttcc ctcgggatgg gcttcatcgg
    86701 cggggttctg cccgccctcc gggcctcgcg cctgaacatc gtcgacgccc tgcgggccgt
    86761 gtagaaaacc cggccgatct tcgttcaagg gtccgcgatc ggaggggggt agcggccgcg
    86821 cctgttccga aattcctccg gccctcaacg gagggcagcc agggcggcga gaagggcctc
    86881 cacgttctct tccgtgcacg acgcgcccat gaggccgatc cgccagacct tccccttcag
    86941 cgctcccagc cccccgccga tctcgatgcc ggcgctgacg aggagcttcc ggcggacggc
    87001 ggcctcgtcc attccctccg gaacgaggac ggcgttgatc atgggcgcgc gatcggcctc
    87061 ggccacgagc atcgagaatc ccattttctg gaggccccgg accagggcct ggtgcactcg
    87121 ccggtgccgc tcccaccgcg cttccaggcc ttcctccagg acgatccgga gggcctcccg
    87181 cagggcgtag atcatgctga tcggcgccgt gtggtgatac acccggtccg gtccccagta
    87241 cttgaggatg agggtcatgt cgaggtacca ggactgcacg cgggtccttc gcttctccag
    87301 gacctcgacg gcgcgcgggc cgaaggtgat gggggccaag ccgggggggc agctcaggca
    87361 cttctgcgtt cctgagtagc aaacgtcgat gccccaggcg tcggtctcca ggggggcgcc
    87421 ggcgagggac gtcacggcgt cgacgaggaa gagggcgccc cgggcgtggg ccgcctccgc
    87481 cagctccggg aggggctgga ggacgcccgt agacgtctcg gcgtggacga ccgccacgac
    87541 cttggcggga aaggccgcaa gggccctgcg gacggcggcg gggtccacga gggatcccca
    87601 ggccgcctcc acccgctcaa gttcggcgcc gcagcgcacc gcgatgtcgc acatccgctc
    87661 cccgaaaatg ccgttgacgc acaccaggat cctgtccccc ggttcgagga cgttgaccag
    87721 ggccgcctcc atcccggcgc ttcccgtccc cgacacgggc agggtaaacc ggttttccgt
    87781 ccgcagaacg gcccggagca tctcctggac ctcttccatg atggcgagaa aggcgggatc
    87841 gaggtggccg atgacggggg accccagggc cgccatgacg gccgggggga cgttgctcgg
    87901 tcccggcccc aggaggatcc ggtgcggcgg gatcaggggt tcgtagcgtt tggacatgcg
    87961 gtctcctttc cgtgcaggag gcggtcccgt cagccccgcc gggacagggc ctcggggttg
    88021 acgaggtgtt tcgggacctc tccccggagg gcggcgatca ggttgtccgc cgccatgacg
    88081 gccatccgct cccgggtcgc cacggaggcg ctgccgatgt gggggacgac gaggcagttg
    88141 ggaaggccga ggagggggtg atcctccggc agaggttcgg gatccgtcac gtccagggcg
    88201 gcggcggcga tgacgccgtt ccggagggcc tcgtacagtg cctccgggtc caccacgccc
    88261 ccccgggcgg tgttgacgag cacggccgtt tccttcatgt tgcgtagagc ggccccgtcg
    88321 atgaggtgcc gcgtctcggc gttgagcggc acgtggagac tgacgaagtc cgatttccgg
    88381 aggagttcgt cgagggtggc gcaaaacttc gccccggccg cctcgctggc cgccgtctcg
    88441 ccccgggggt ggtagtaaag gacctccatg tcaaagcccc gggccctccg ggccatggcc
    88501 tggccgatcc ggcccatgcc gacgatcccg agggcggccc cgtggatgtc ccgtcccagc
    88561 aggacgagcg gttcgaaggt ctcccatttt cccgcccgga tgtagtccac cccttccacg
    88621 atccgcctcg ccgccgccat gagcagggcg aacgcgaggt cggccgtggc ttccgtcacg
    88681 gcgccggggg tgtggccgac gaggattccc cgggcggttg ccgcggggac gtcgatgttg
    88741 tcgaaaccga cggcgtagtt gctgatgatc ttgagcccgg aaccggcggc gtccatgacg
    88801 gcggcgtcca tccggtcgtt gaggagggac aggatcccgt ccaccccccg tacctgctcg
    88861 aagaggactt tccggtcggg gggaaaccgc tcctcccata tccgggcgtc gcaggctgta
    88921 cggatccggc cgagccctgc ctcggggatg agccgggtga cgaaaacctt cgcttttccc
    88981 atggaccggc tcctaggact ttgacagggc atcgtagacc cggcggttga agggggtgtc
    89041 gatccccagg gcctgtcccg cccggaccac gtatcccatg aacgtctcga tctccgtttt
    89101 tctcccgttc tccacatcca cctggaagga ggtcttcgtc tcgtaaggga agcgtgccac
    89161 tgtttccagg gcctgggcga cggcgttcgc ggggaggtcg attccccggg cccgggccag
    89221 ccggtccacc tcgtccatca tcccctcgag cagacggagg gcctccggtt cttccaggat
    89281 gccccccagg gattttccga agagggccgt cgtgcccgcc accggcgaga tgaagaggaa
    89341 ctttttccag acctccacgg cgacggccgc ggtcagaacg gcttggatgc ccgcctccag
    89401 aaagagggat tcgatcgggc ggaagggctc caccggcccc gtttcggggc cgaagaacag
    89461 gctgcccgcc ccgcccagtt gctggaccac cccgggcgcg gcgatgcggc tcgagatgta
    89521 caccgtcccg ttcaggaccc gggcgcgggg caggatcttc cccagccgct ccgcggtgtc
    89581 cacgccgttc tcgaggggga tgacgacggt cgcctccccc gtgaccgcct cgagggcccc
    89641 ggcggcgccc tcgaggtcgt accccttgac cgtgatcagg acgaggtcga gggggccgag
    89701 gtctcccggg cggtccgtgg cctgggccgg ccgggcggtg aactccccgt ccggggtgac
    89761 gaccttgaga cccccgttcc ggatcgcctc gaggtgtgcg ccgcgcgcca cgaaggcgat
    89821 ccggtgttcg tctccggctt cgtaacggcg ggccagccgc cccccgtagt agccccccac
    89881 gccgccgatg ccgacaattg cgatgttcat gggctctttc ctttcttcgc gtccgacgcg
    89941 agggagatga tgagctcgta gacgggccgg aacatcgtcg ctacccgccc ccttttgggg
    90001 atcatgaaca cgaaaccctt gcgatcgcct ggaaccaggg cgatgtggcg gatcgtcccc
    90061 ggatcgggat gacgggcgag atgaccgcgg aaccgttccc gcacgttcag gttatcataa
    90121 tagaggataa aatccagctt tccccaccgg accgactcct tttccagccg ggcgtcgttg
    90181 gcgatcttcg gtccccggaa acggctgtag gagcgttcga tgagatggcc ctctccggga
    90241 acgtccgacc tcagatgaat cgtgacgaag agacggtcga accgcatggt gagaaaggag
    90301 atgggcaggt agagccagga ctggcggggg aggagcgtga tcgtcgcatc cacccgcgac
    90361 accggacctt tccgtttgag gaggagattg gcgtgatacc ccacgacgcc gccgatggtc
    90421 gtgtaggtct gatcgtcggg cttcaccgtg tccaccaggg cctggaaggc gtcgaggaaa
    90481 atcctccggt ttcgtctccg gccctggaaa taccccaccg tcaggaggat cgtggtgacg
    90541 acgaacagat agaacaaggg ttcccgcagg atgtgttcgt gttcgaacat gttcagaatc
    90601 gctccccgat gtcgttctcg atccggtaaa ggaccgtttc tttcccctcg taaagggcct
    90661 ccgaaagggg cacccgttcc acgggaacgc cgggtgccgc cttccgcagg gcctgctcga
    90721 cccgctccgc catttcccgg ttgcgctccg tgaccttgtt gtttaccagc aggcggagcg
    90781 gcaggcggag ctccgccaga ccctccgcga gccggcagga ttcctggagg gacagcaggt
    90841 cggggttgaa cacgaggacc acgctgcaat cctcaccccg caggaggccg ttcaaccggt
    90901 cgtaattcct ccggagggcg cggagcctct ccaggatcgc atcctctccg ggatcgtatg
    90961 ccagtcgctc ttccgccggg gtttcccctg gcttcagggg cctgcggatc tttccgatcg
    91021 tgtagcgctt ttcgagaatc gtctctcgga tgcggatcag ccggtcgatc caggtgaggg
    91081 tcacgctggg gagagcgagg aaccggaggg tgagccccgt cggcggggtg tcgaacatca
    91141 cgtagtcgaa gccggtttcg gcgttcaggg ttcgctcgat gctcgtgagg agggcatact
    91201 cctcgattcc cggagagtac ttgaggacgg aaaagtagtc gtccaggttg agcacctgga
    91261 gatagcggta agtctcaccg aggacccggg tttcccgttc gagatactcc cgggacagct
    91321 ttttaagatc cacctcctgg atcgtcaggg tgtccgagat ctttttcctg ccgttgcccg
    91381 ccgtaacgcc gaaaatgtcc ccgaggttgt gggccgggtc gagggactgg acgagcaccc
    91441 gggcccgccg ggagatctgc cggccccagg cggcggcgat ggtggacttt cccacccccc
    91501 cctttcccgt aaagaacaaa aacttcatcc gtcgattccc agcatgccgc acatttcagc
    91561 gagcatatcc ccggcgtcct ccgcccggac gttcaggatc cggatctccc gttccatgaa
    91621 gggcaggagc tccacggcca aaaacgtcgg gggggccggc aggccgatga aggatccgag
    91681 gacgaggagg gcgaagatgt tttccagctc ccttgcttcc acggcggtca gcgccgcggc
    91741 gctttcccgg tggtggtcgt caagccggac caggaattcc cgcaggagcg aaatcgggga
    91801 gaatttcatg cacgcatcca tcaccgggga aaagacgcgg cgcgggggct tcgggctccc
    91861 ccgcgccgcg ccgtccgttc acttggactg aacttgttca cggtagtact tcttcacgaa
    91921 atccacgatg aaaatgaagt tcatgatgag catgacgatc gaaatgccca tgacggtcca
    91981 tccctggacg ggcgagctgg cgatgttcgc cggcagggga acccacatga accacaccag
    92041 ggcggcggtg accgttaccc acaggatcca ggcggggatg acggccacat gacagaatct
    92101 ggactgcagc ttcgaatgga cccagcacgc cccggtcatc agggcgatgg aggcgatcaa
    92161 ctggttcgcc gcgccgaagg aaggccagag gatggtgaag ttcttgctgt aggccaggta
    92221 gatcccgatg aaggccggga tgagcgaggc gatccagcgg ttcgtgagaa agttgtagag
    92281 tcccgccgac gtccccttga gggggagcgc catctcggag acgcagtagc gggccagccg
    92341 attcgtcgtg tcgagggtcg tcagggcgaa ggcggcgacc cacatcccgg ccagcacctt
    92401 cacgtactgg gtggggatca ccgacagcca cgtggcggcc accatgtcgg cgtaggactg
    92461 gatgaagagg ttcgcctggg aaagcccgag tttcgaggac aggggggtga actgggtggc
    92521 ccaggtggag gcggtcaggg tgaactcgat gcccttctcc gccgccgccg tctgcagggc
    92581 cgtgaacccg aaccccgcga tggccaccac gacgatcgtc gacaggaacc cctcggtgaa
    92641 catggcgccg taaccgatga gcaggccgtc gctttccttg gtgagctgct tggaggtcgt
    92701 tcccgaggcc accagggagt ggaaccccga cagggacccg caggcgatga tcagcggaat
    92761 cgccggccag aagggggtcg gcttgccgcc cgtgaggatg ggggagaaac tcgtgaaggc
    92821 ggggacctcg aacccccgga aggcgaagac cgccgcgagg agccccagga tgagtccggc
    92881 gtacagcagg aaggagttct ggtagtccct cggctgcagg aggacgttca cgggaatggc
    92941 ggcggcgatg atgatgtaga agaacaggat atacatccac gtttcgtagg tcgccggcat
    93001 gggaaactcc acgccgagga agatggcgac ggcgagcata atgacgccga tgacgctcgc
    93061 cagggtgaag ttcatcttca cgcggtagat gaggacgccc aggatgaggg ccgcgacgat
    93121 tgaccagacg taggcggagg cgatggcggg cgtcttcacg aacatgccgc cgacgacggc
    93181 gctgaaagcc gccacgacga ggatgagcag gaagaagacg agccagtaga aggcggtccc
    93241 cgttctcttc cccatgaggt ccgaggccac gaactggatc gaccggccgt cgtagcggac
    93301 ggacgccatg agggcgagat agtcatggac ggccccgatg aaaacgttgc cgaaccagac
    93361 ccagagaagc cccggcaccc agccgtaggc catggccagc gccggtccga tgatgggcgc
    93421 cgctccggcg atggaggaga aatgatgacc gaacaggacg gccttgcgtg cggggatgta
    93481 gtcgacgccg tcgtacagcc gggacgacgg cgcctcgttg gcgtcggtcg cccccaccac
    93541 atcccgctcc agttttttcc cgtacaggaa atagaggagg acataaatcg ctaaaccgac
    93601 caggataatc agggtagtca cgctcttacc tcctttctga agccatgcat gctacggttc
    93661 ggtgcaggaa gcccctgccc ggacgttccg gacgcgggga catccccttc gttgattctg
    93721 gaaaaacggc ttgacggtcg ggacgggact caagagggtg gaaacatcgt ttcgaaatcc
    93781 ggcggacttt cccatggagc ggctcccttg gggcggatca tgccagctgc ctgtttctga
    93841 acgcgtgcac tgtaaacaag tcaggcccgc atgtcaaaga aaatttggca acctgggatc
    93901 gcgtcttgct tttcccgatc ggccggggta tggaacagag gcgtgtccga cgggagccgg
    93961 agggggcgga ccgcgtgccg ttttgcgcgc tcagccgggt ccggcgcggg agaacggacc
    94021 gcggtccgga ggatgaacgg gcatgagttt cggaatcggg atactggccg gcgcactcgg
    94081 cgcgctggtc ggcctgggag gcggcgtggt gatgatcccc ctgctgaccg gctggctcgg
    94141 attctcccag caccaggcgc agggaaccag cctgctcgcc ctcatgttta ccgggctggc
    94201 cggggccgtc gcctatggcg ccaaccggtc cgtcgactgg ccggccgccg ccctgctcgt
    94261 cctgcccgcc atgctgatgg cctgggtcgg cgcgcggtcc tgcaaccgcc tgccggaacg
    94321 gaacctccgg cgtcttttca gcgcgttcct catcgcgatt tccgccctga tgctcctcaa
    94381 gcccttcctg catcccgccg catccggcgc ggcctccgtc ggcttcgagg ccggtgtcct
    94441 ggtggtgacg ggattggtca cgggatttct gtccggactt ttcggcatcg gcggcggggc
    94501 catcgtgatc gcggcgatgg tgcttgtgct cggctacgga cagcacccgg cacaggggag
    94561 cgcgctcgtc gccatggttc cggtcgccgc cgtcggagcg tggtgctacc gggacttcgg
    94621 gcgcgccgat gccggcgttc tcaagggcct tgtcccgggc atcctcctgg gggccgcggg
    94681 cggcgcccaa ctggcccatc tgttccccga cgccgcactg cggatcatct tcgccgtcgt
    94741 cctcgccggt acgggtctcc agggtctgcg ggcgaaagtg ggagaagcat cgtgtcgggc
    94801 ttcggggggg agggcgggat gaaaatgagt gcaaaagcct tcgatcgggc cgtcgcgcgc
    94861 gcggtggacc gcattccggc ggagatccgg gatctgatgg agaacgtcgt cgtcgccgtc
    94921 cggaagcagc cgacgcagga aatgctcgat gcggtcggga tgaccgcgga cgacccgccc
    94981 ctcggcctct actggggaga atccctgctc gagcgttcca gcttttcgcc cgtccagtac
    95041 ccggacacga tcttcatctt tcaggagccg ctcgaagaaa tgtgcgaaac cctcgcggag
    95101 ctggagcggg agatcgagat caccgtggtt cacgaggtcg cccatttcct cggtatggac
    95161 gaggatcgcc tggcggacct gggatacgcc tgagggggtc gtggccgcgc ggtcaccgct
    95221 ttccgacccc gccgccgcgc ttccgcttcc tgtcgatttc cgcgatggcc ctgcccgcgg
    95281 ccgccttcac ccttgggctg aaggcccgga ggctcaggaa tccgatctgg cgctggacgg
    95341 atgtcagcgc cggcagggcg gattcctcgc cgatggcccc caaggccgcg cagatctttt
    95401 cttccagctc ttccctggcc gccgaggact tgacgggttt cgccttcagg agatcgatca
    95461 agacgggtac ggccggcgga tggcgcaggc tgcccagcag gggaacgatg tcgagctgga
    95521 gcgcttcctg cgcctgggga agggtctccg tcaggagcgg cccccgctcg tcgccgccga
    95581 tgccgttgat acttttcagc gtctccttct gaacgcgccg gtcggcgtgg aggagcagcg
    95641 gctgcagggc ccggacatgg atgtccccgc cgatccggcc caggaggcgc gccagattcc
    95701 gctggtagta ccagacgccg tcctgacgaa gttcggtcag caccgcggat acggccaggg
    95761 ttcccatgtc gttgatgagg ttcatgatcc ggatgcgctc ttccgcgttt tcactcttcc
    95821 cgagcagctc caggaaatgg gcggcggcag gttccaagcg gaccaacagg cgtccggcct
    95881 cgtcgctttc gaagagctgg ccgggctggt cgagcccctg ccggtcgcgg gtttggaaaa
    95941 cgttgaacag gatatcgatc agctccgcct gcgagaggtc ccgcaggacg tcttccgcca
    96001 gctgctggat cgtatcgttc cgctccagcg cgccggtatg aatctggtga aaggttttca
    96061 cgatggcgcg gcaggcgctg aaccggcgct cgcggatcag ccgttgcccc agttccccca
    96121 cctggcggca gagctttgtg taggcggtgg tggcggcggt ttccttcccg atccacgcgg
    96181 tcagcttttc caggttgcgg acgaccggtt cgtagcggtt gtcggaaacg agtcccggaa
    96241 ggatccgggc caggttgtcc gacgcctgtt cgcgctcggc cgcccgttcg ctcgccaggg
    96301 tcgcaaacag ctcgtcgatg atctcctcgg cttcggcgcc gcgcccgcgg gcgtaaagga
    96361 ggccaatccg ttcggcggga gcctccttgg gagccgccgc cggcgccgcg acgggggtgg
    96421 ccgcggggcc cccgtcgacc gagaggatcg gctcgccctg tccgtggcgc ttgatcatcg
    96481 tctccaaggc cgcaacctgt cgttcgaaga tccccgtatt ccgacccctc ggcgcccgcg
    96541 cccccggcgg gagatcggcg tccgccaacg gttccgcgag agcggggtct cccgagggcg
    96601 ccccggggtc ggtgggtctc cccccccccg ccggagggag gttgcgatgc agcaggtcgc
    96661 ggacgtgctg gaagaaacgc tcctcgaacc cgctttccgt gccgcgggac aggagggcct
    96721 ggatgacccc ggcgtccagt tggaccacgg tcctggcgat caggagcgag agggactccg
    96781 cctcttccgg cgcggccgtc tcgcccagca tccgcatcat ggacccaagg cggtccgaaa
    96841 atggcccctc cggtggtgat gcatccccgg cggcgatctg cgcgatgccg gcccgaagga
    96901 ccctcccgaa ccactcggga tcctgcgcca tctcgcggat cctgacggga tcgagagacg
    96961 cggcgcccgc ggttccggcg agataatcga ggatcgactc ctccccgatc ccaccggtcc
    97021 cggggaacgg ccggtccggg tccatcgcga cgaagcgctt ctcgcccaga cgaatgtgcg
    97081 ggaggtcccg cgcctccaag accttccgga gcccgccccg ctccgccagc atctccggtt
    97141 tttccgtcag aatatccaga aaggccagca gttcgtccgc gccgacgccc cgggagaaca
    97201 cgatgttgtg ggcgccgacg ttcatcatga gctggagaaa ggcggcgacc tggggtcttt
    97261 cccggtcttt cgggcccagg ggggttccat tgacgaggag ggtctgttcc gcttcggcga
    97321 acgtcacctc ctcgtcgttt tccaggacct gccccagggt atggtgcagc ttttccaggg
    97381 tggtccggat catggcgctc gtgggaggat agaggcgcag attggtcagg gcggtattga
    97441 cggcgatgag gatctcgacc gttgggctca gcgcgcttcc gtctgacatg ttccctccgt
    97501 gtacgcgtgc gcccggtcca cgcggggcgc aaagctcgcc ccgggtttcg ggcttcgtgg
    97561 gatcttacgg gaaacatcgg cggctgtcaa cgaccagggg gcgcgcgggg ccgggggatg
    97621 cgggatcatt ccttcagacg gtattgaaag cgggcgacga tgtgcagccg gtcgagccgg
    97681 aacggccggg ggaggggccc gaacggcgag gaggtccgga tgacgtccag ggcggcgcgg
    97741 tcgagcagct ccgagccggc ggaggtcacc acgtccgccc cggcgaggct gccgttcgcg
    97801 gcgatggaaa aacggaccac ggtttccccc tcgtggccgc gtgagaccgc ctcggggggg
    97861 taggcccagc gccggttgat ccggtacttg accttgagca ggtagggcgt gtaggggctg
    97921 tccgggttct ccagggcgac ggtgtcctcc tgcggaacgg ggggaagggc cgccggcgcc
    97981 ctccgggcgg cgggagcggc cggcttcgtc gcctgttcct ccgtcgcctt ctccggttcc
    98041 ttcaggctga ccgtgagcgt ttccgggtcc cggctctccc cggtcatgtc gatgaatccc
    98101 gtgagagaca ggacgaccgc gtgggcaaag atcgacgcca ggatggccag gacgagcagt
    98161 tttttggagg tcatgggccc gctccgttga ggtgcgggaa ctatagcaga gatggggcag
    98221 gaatcaacgg gaaatgcgca cggattctcc ttgattatcg ggcgaagccg ttgtacacaa
    98281 cacccgtccg ccgaaaaacc gcccgaccct cacgcaccgg agacgagatc catgacgctg
    98341 cgccggaaaa tcctgatcat tttcgggctg accctgttgg ccctgatcgc gatcctttgg
    98401 ctcagctcaa acagtgatct gatcgtgccc ctgcttctcg tcggcctcgt cggctttgtc
    98461 cttctggaaa aattcgccct gcgccctctc gcgggtctcg ccggggagat cgcgaggatc
    98521 ggcgagtgcg gcgacttctc cgagcggatt tcgacccagg gtcgggacaa cggggcgggg
    98581 atggcggcgg ccgtcaaccg catgctcgat gcgctggaaa agacgcgggg ccagttcgaa
    98641 accgaatcgg cccgctaccg ggcgatcatc gaactccagt cggaattcat cggccgcttc
    98701 cgggcggacg ggacgatcac cttcgtcaac gacgccttct gccggctggt cggaaaaccg
    98761 cgggacgaac tgaccggaac gagcctccgc accctgattc ccacggacga ctatgaacgg
    98821 atcctgcagc aggtgcgcgt cctgacgccc gcggcgccgg atctgcccga caacgaacat
    98881 cgcatcgcct tccccgacgg ccgggcgcgc tggctgctgt ggaatgcgcg ggccatcttc
    98941 gacgccgggg gccgcttcct ggagtgccag gccgtcggtc gggacatcac ccgggaaaag
    99001 gagctccgga gcgcctaccg gcggagcgtc tcgacgatcg agcaggccaa gatcgaatgg
    99061 gagggctccg tggattccct ggaggggttg atcctcctgg tggacgatca ggggcgggtg
    99121 gtgcgggcca accggagcct ggaggcctgg tccctgggat cggtaaaggc ggcgatcggc
    99181 cggcccctgc acgaggtcct ccatcccggc tgttccgaat ccgaatgcgc cctggcgtcg
    99241 tttttgaagg cgtccctggg gagggtccgc gtcggcgaga cggtcgagac ggaactggcc
    99301 gatccacggt tcgaaaagac ggtccaggtc cggctgcgga ccgtttccct cgggggtcgg
    99361 cgcaacggtc cggcatccgc cgcgatcgcc atcgtcctcg ttctggacgt caccgcccgc
    99421 gtgcgggcgg aagagacctt gaaggccgtg aacagccagc tcgaggaggc caaccggcaa
    99481 ctggggatgg cctatgcccg gatgcgcgac gagaaggacc atctgaagag acagctcttc
    99541 caggaggaga tggggctgct gt
//
LOCUS       DAOOLJ010000003        84157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_132_length_84157_cov_16.324380, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000003 DAOOLJ010000000
VERSION     DAOOLJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 84157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 84157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84157
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_84157_cov_16.324380"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLW16_00910"
     CDS             <1..645
                     /locus_tag="PLW16_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB69494.1"
                     /translation="PVCVIDDGSTDATAARLEEIVGVAVLRHETNRGKGAAILTGFDA
                     LAGRADWAVTLDADGQHDPNDIPGMIRAIPAGSRPIVVGRREGMIGADVPWTSRFGRK
                     FSNFWVRAAGGPVLSDTQSGLRIYPLPEAARLGVRARRFQFEVEILVRAHWRGIPVIE
                     APVGVSYTPGMKRVSHFRPFVDFCRNAETFGRLIALRILRFPGRGGPRPTSDEG"
     gene            650..3778
                     /locus_tag="PLW16_00915"
     CDS             650..3778
                     /locus_tag="PLW16_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB69495.1"
                     /translation="MFFRRYRFRWFLILAVFLILGGLFIYTARGIRIETDILASLPHR
                     DPVLADAHRVIRHLPVQDRLVCDLEIRGGDSETLVEAAERFETGLTRSGLFRKVGLGE
                     MQALGPELVAHVVGHLPLLFDERQLREEVVPRLAPERIERQLAENLDLLQGLEGIGQA
                     ELVARDPLGLRTLVMARMAQLLPAREARFHRGQLLSNDGAHLLMTAELAGAATDTRFS
                     RDIPPLLDDLTKEMNAAYRSRGVSFVVTPVGAYRAALDNETAARGDMRTAIWLTTIGI
                     ALLLVLTFPRPLIGLMAILPSTAGALSALFVCSLIFPRLSILAVSFGGAIMAFTVDLG
                     IAYLLFLDRPFETTGKQAAREVQSVETLAVLTTIGAFLLLTVSEFSVLAEIGVFAALG
                     VACAYAFVHVVFPLIIPVMPPAKRVRTSPLATLLDRIVSSEGRGRGRLAAAALLFGVM
                     LCFSRPVFQVDLNAMNSLSAASIAAEKRVQAVWGNLTSRVYLLTEGAGPEALQDKSDA
                     LAPWLEEDERRGVIRAPFVLSDLFPGEARARRQAEAWRAFWTPERTADVARSLKRSGD
                     GMGFSPAAFTPFLNSLTAAPPATPDVPERLAGLLGLSPGPEGPVQVTMVTPGPAYDAR
                     AFFDRYAATGLVRIFDAGLFSKRLGDVLVTLFTEVALIVAMGITLVVFFFFLDWRRTL
                     IVLAPVVFALVCTLGTLKLLGRPIDIPGVMLWVVIMGMGIDYGIYYVCAYQRCLDEHD
                     SSMRLIRLSILLAAATTLIGFGVLAIAEHAVLKSIGLTSFFGIGYSLLGAFVLVPPLI
                     RRVLAPVALPPESFPAGSPRHESRVRLRYRHLAAHPRLFARLKMHLDPMFPHLASFVS
                     APRRILDIGCGIAVPSVWLTELYPQARVFGIDPDEERIRVARQAIGPRGEMRTGRAPE
                     LDDLPEEADTALVLDLIHMLDEAAWRRTLAVLHAKLITGGRLILRATVPADRPFPWKR
                     RLEEFRLKSHRLAPHYRSRTAIMAGIAAAGFDLETVEADGPGSEEVWFVAVRSPSDPV
                     EEASRTSEGVRP"
     gene            3775..4683
                     /locus_tag="PLW16_00920"
     CDS             3775..4683
                     /locus_tag="PLW16_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB69496.1"
                     /translation="MKDRFYRFLTFLTRHLGAWVFLLVAWFIATGYFLFFPRRVAVSV
                     RFYRRLLPGRSRFHPLWCAWRQYHHFVRVFLDRFLLTEGADLPTTYEGWDHLVAAAES
                     GRGGIVLMSHVGNWEIAAHLLKHYGRSHPSMKLLLYLGEKHREQIERRQKKSVARNGI
                     RVIAVPEEGGSPMDIVEGLNFLREGGLVSMTGDRLWGRDPRTVDVRFAGARARIPETP
                     FVLALMSGAPLLIFFAYRTRGGVYQLITKPPLTVRAASRSERTDAITAAAQTYADTLE
                     ETVRAHPFEWYHFEPFLLEDPAAGPG"
     gene            complement(4711..5487)
                     /locus_tag="PLW16_00925"
     CDS             complement(4711..5487)
                     /locus_tag="PLW16_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB69497.1"
                     /translation="MSQEIVLTEARGPVSWIIFNRPEVYNAFNLDLAKGAFSALVRAV
                     DDPATRVIVFAGAGKSFSVGADVTEVTAAPDPTRIIGELATIAHRAIVEVRQSPKPVL
                     AAVNRLAAGYGLALALASDVRVATERVRLRYAYSTIGLTGDGGINWTLPRLIGTARAL
                     EVALLGEDIHEREAEKMGIITKFFPEDRLIEETQAIAERIAAIPAKTASAIKRMVYGS
                     AGMDLLVHLSVEHAALLEATTAPDFGIMLDRLLAGFSKKG"
     gene            complement(5704..7050)
                     /locus_tag="PLW16_00930"
     CDS             complement(5704..7050)
                     /locus_tag="PLW16_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB69498.1"
                     /translation="MIESIFSRLSDPDRPYVDGVSTLGDICLQARGIRDAFTRLREPE
                     DAPVCLCLQDRSLLLAALLASLAGGPRFILPHAYDRRVLEEVREVLPFHRILTDTAPD
                     IPAGVEAVPCETRRPEHGDLAPVRSPDEVFLTLFTGGSTGKPRSWSKTPRNLFGEAFF
                     LARTLGIRKSDRLLATVPPQHIYGLLCSVLLPFAAEARILPGTCLFPREILAALASAD
                     VTVLVCVPIHYRALKTADLKRFSLRLALSSAGAVDAEDGAFFRERTGLPVTEIFGSTE
                     TGGIALRTDPSAAGVCIPFRVVDWKIVSDRLAVRSAFISPDLPRDAEGYFLTSDRVAP
                     AQDGFRLLGRADGIVKVGGKRVDLEEVRNRLRRLPGVKDAHVLALPLKRGRQVELAAL
                     VAGDLDPAELKAAVQTMGEPHARPRRLRIAAEIPTLPNGKIDREKIEQLLSDVTPS"
     gene            complement(7054..7755)
                     /locus_tag="PLW16_00935"
     CDS             complement(7054..7755)
                     /locus_tag="PLW16_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPB69499.1"
                     /translation="MNDTIAARIRRIRKDMADAAAKSGRPPSAVRLMAVTKTVDEARI
                     REALAAGVDVLGENYVQEARRKIERLGTDVPWHGIGHLQTNKAKQAVKLFTLIHSVDR
                     IGLAEEIDRRAAAAGRVTHILIEVNVTSEASKSGVPLSQALPLVHAVSALKNVSVRGL
                     MTLPPWSDDPEASRPCFAALRRLRDQIRAECLECCVMEELSMGMSQDYRVAIEEGATI
                     VRIGRLIFGERPARG"
     gene            complement(7752..8408)
                     /locus_tag="PLW16_00940"
     CDS             complement(7752..8408)
                     /locus_tag="PLW16_00940"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPB69500.1"
                     /translation="MHLQDGNSRAGAEPSAARIAMTGPLILASASPRRVELLRRLGLD
                     FETRTSGVDETFLPGEAPADHCRRLARTKAETVARRHPHAWVMGADTIVVIGDEILGK
                     PGSPPEAMDMLGKLSGREHRVFTGFSLCRHDRGLSLTGAVESSVCFKEIPADEMAWYV
                     RSGEPFDKAGAYAVQGRGAFFIREIRGSYTNVVGLPLAEVVDTLKQAGVIVFAPEETP
                     "
     gene            8538..8660
                     /locus_tag="PLW16_00945"
     CDS             8538..8660
                     /locus_tag="PLW16_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69501.1"
                     /translation="MRFWQGTGSGMIGVGLILLLAAPVMAATRHVQMDIPGCMT"
     gene            complement(8910..10025)
                     /locus_tag="PLW16_00950"
     CDS             complement(8910..10025)
                     /locus_tag="PLW16_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPB69502.1"
                     /translation="MHAEIGNPDGGKSIYAVIMAGGKGTRFWPRSRERMPKHLLDIVG
                     TRTIIQETLARLSPLIPPDRTLVVTGASHAEELMRQLPEVPPDNILIEPMGRNTAPCI
                     GLAALAVNRRSPGAVMAVLPADHLIRDEARFLHVLAAAVRVADREAALVTIGIAPTGP
                     ETGYGYLENGEPAGTVTGETVYRVRSIREKPDRPQAESFLRQGNFSWNSGMFVWQAPV
                     ILDHIARHLPDIYRALREIDVAWGTPRESDVLRDAYAAMTPISIDYGIMEKARDVLLI
                     KGDFGWSDVGSWDALWEVSPRDAAGNAVRSGAHFVGVEARNSFVFSPKKLVALVDVED
                     LIVVETEDALLICRRGASQDVKKVVNLLAEGPGRAYL"
     gene            complement(10030..11253)
                     /locus_tag="PLW16_00955"
     CDS             complement(10030..11253)
                     /locus_tag="PLW16_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HPB69503.1"
                     /translation="MKITEIELYHVHIPLKHTFWPTWIPGYPQTHNRFTLIRLVTDDG
                     IEGWSAGTAMGEERQGLGDLLGGYIIGADPTDIDRIQSLLKQAGYLGWLNPWIEPACW
                     DIMGKAQGKPVYELLGGEARAIEAYCSFGELHEPARRVEEILSLMARGFKTFKLRAKA
                     KTFAEDVQLIEEVRKGVGDGPTLGVDANQGWLVSIVDRIPAWDLNRAGDYARACADNG
                     IEWLEEPLDWHDYDGLAALKKASPVKISGAELNHGWDEIKVMLEKDCFHVYQPDATFA
                     GGIAQVRQVIDACHRHDREYSPHTWTNGIGFYINWNLVLADRGNTHPLEYPLEEPSWV
                     PEFRDGLIAPILPDKNAMLWPFTRPGLGFEIDRKMLKKYARRFYRQTTRGLAFKVIRE
                     KGLKAALEIKKRKEG"
     gene            11501..12694
                     /locus_tag="PLW16_00960"
     CDS             11501..12694
                     /locus_tag="PLW16_00960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPB69504.1"
                     /translation="MTKKPVYPVVTLKAGREDSLRRGHPWLFSGALARIPEGLDPGEV
                     VTVASASGDALALGFYNPQTDIAVRILTRDCRAGIDAAFWRERLQVALALRRRVVPAD
                     TTGYRLINAEGDGVPGLVVDRYDRWLVLSIATAGMDGFREDWVRILEEALAPEGIYER
                     SEGAARRREGLSDRSGVLAGAAMPEVVPMSENGFPFAVDVEAGQKTGFFLDQRPNREL
                     SGRLAAGAGCMLNAFAYSGGFSVYGIRGGAGRVVSVDISERATALAKRNLALNGLSEE
                     THPVLTADVFKYLRETDETFDLVVLDPPAFARSRKDLARAARGYKDINLQAMRRLTAG
                     GLLLTFSCSNPLDQDLFEKIVLGAARDAIRTARVLQRLGPGGDHPTDLGHPEGRYLKG
                     LLLCL"
     gene            complement(12707..13483)
                     /locus_tag="PLW16_00965"
     CDS             complement(12707..13483)
                     /locus_tag="PLW16_00965"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPB69505.1"
                     /translation="MEVITEKNGAVWTVIINRPETRNAVNRDTADRLVAAFSEFDRDE
                     CAAAAVLYGSGGAFCAGADLKAVARLSEGQANRLNTDMTAPGPMGPTRMKLSKPVIAA
                     ITGHAVAGGLELACWCDLRVMERNAVLGVFCRRFGVPLIDGGTQRLPRLIGMSRALDL
                     ILTGRPVGAEEALAMGLVNRVVEPGAARTAAEALAAEIAAFPQRCLRSDRLAAYEGFD
                     QAFDAAMENEFRRGLEVIFSGETQEGAARFRNGTGRHGRF"
     gene            complement(13527..13925)
                     /locus_tag="PLW16_00970"
     CDS             complement(13527..13925)
                     /locus_tag="PLW16_00970"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPB69506.1"
                     /translation="MIKKTLWVCLLLSILMVGCDTALTIGNRTVGVTSGDFLYTDGFL
                     HSRYAASFDRVWEACEQMLSDMKATQVKPVRKIAQGQIQAVIQDEKVSIEVEYVARNE
                     TTVAIRTGVSGNNLASRMLHERIAQQLAKP"
     gene            complement(14056..14904)
                     /locus_tag="PLW16_00975"
     CDS             complement(14056..14904)
                     /locus_tag="PLW16_00975"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB69507.1"
                     /translation="MASPRKTSLLCPGCRKLISADEPACPHCGLTRPGAPWRAALGVF
                     GLASRQLVPGLIAVNITLYLLSLLLTPAGIGLAPSPFSFLSPDGRALYRLGATGVLPI
                     AYEGRWWTLVTASFLHGGLLHLFFNMAALWQLGPFVQQIFGTHRFLVLYVASGTAGFL
                     LSYLAGIPFTIGASAGLCGLIGAILYYGKSRGGFWGESVYRQATGWIVGLVLFGLLVP
                     GINNWAHGGGLVAGLLMAFALGYKERNGEPAWLAPCGGVTAIGTLVVLVWATVRAFLS
                     DVPAPF"
     gene            complement(14911..16068)
                     /locus_tag="PLW16_00980"
     CDS             complement(14911..16068)
                     /locus_tag="PLW16_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPB69508.1"
                     /translation="MNPEPLHRFQYPGKELVLVGTAHVSRDSAELVDRVIREEKPDTI
                     CIELCAARYEALRQKDKWQEMDIVRVVREKRTALLLAQLLMASVQNKLAERFHIQPGE
                     EMLRAMARAEETGAELVLADREIRITLLRVWRSMGFWARTKFISEMVFSLFFSEEITE
                     KDIEKLKQQDVLEVALETVGKKLPELKTTLIDERDQYLAHRISHAPGQKIVAVVGAGH
                     VPGIVKNLDRIIDLNALNRIPPPSLWAKWAGWGFTLAILGLFVAGFFASGSRASLTMM
                     AWWAAITAACASVGAILLLAHPFTIAAASLAAPFTTLHPLLAAGWIAGLVEASVRKPQ
                     VRDFLELKNDIMSVRGFFRNKITRILLLVAIVNLTTSVGTFIAIPFIMRFI"
     gene            complement(16153..16902)
                     /locus_tag="PLW16_00985"
     CDS             complement(16153..16902)
                     /locus_tag="PLW16_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69509.1"
                     /translation="MEVCFLGTNGWYDTETGSTVCTLISAPGAVVVLDAGGGLAKLDR
                     HLSPEDPRPVYIFLSHFHLDHIGGLHTLTKFNLPGGLTLCGPAGGRAVLRTFVNAPFT
                     VPLDGLPYPVHLCELPGEAAALPFPVQALPLVHAGLTLGFRLELDGRIIAYCPDTGYC
                     ENAVTLARRADLLITECAYKTGQSSESWPHLNPETAARIAREAGARRLALTHFDARIY
                     GSLPERREAEAAARADFPETFAARDDLRIEC"
     gene            complement(16979..17680)
                     /locus_tag="PLW16_00990"
     CDS             complement(16979..17680)
                     /locus_tag="PLW16_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPB69510.1"
                     /translation="MTTPEETAALKQEILACNGWLSSHGYFGTLRGTGGNVSVRSGDD
                     AFLITPSSLSYDEMTAEDLCLLDFDLNVRAGARRPSVEAGLHAAVYRKRPEIRAIVHT
                     HQPFASVFALINRPVPALFDEVAFKLGEQVEIISYALSGSPELAANVAARMDNHAHAY
                     IIQNHGALLLGADLAEAWLNAELLEKACQVYACALSTGAPVSPLPEAVRNVIRDTRRF
                     LREEKLKTLSGNRSG"
     gene            17892..18812
                     /locus_tag="PLW16_00995"
     CDS             17892..18812
                     /locus_tag="PLW16_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB69511.1"
                     /translation="MKWTGKHVALALGGGGVRGFCHIGVLRILEREDVPIDLIVGTSA
                     GALIGGAYASGQAPRDIQAKVDAYLSSPEFGASTLKSIGLAISPEQKGRWRKARAYVL
                     NRFHLVRAFFRPAVLPTEDFRSLIDYFLPEVDIRETRIPFRAVATDLITGKQVVFSEG
                     PLRQAVLASCAVPGAVEPVRHGEWLLADGGVTSLVPVNAARAAGADVVIAVMVDRDLM
                     PDGNMETAQGIIQRAGEIAANTLEATELAGADVIIRPDVGDLHWMDFSRAGELVRKGE
                     EAARAALKQIDASLPLYRRISRLGRRIFKG"
     gene            18817..19584
                     /locus_tag="PLW16_01000"
     CDS             18817..19584
                     /locus_tag="PLW16_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB69512.1"
                     /translation="MTDSRLFGSAFLKIAHRGYSSRYPENTLPAFEGAVAAGADMIEL
                     DVRLSRDGELVVIHDATIDRTSNGRGRMADLTLAELRRFSYPNAMTAFGRVPIPTLAE
                     VIDRVGAQVTLNVEIKGRRSGSAGMERLIAALLRRKGAVDRVIVSAFDRRALVEMKRA
                     DGQVRTGLLYDRGWRRFGEDVRRLGVYSVHPRLSAIDAEALRWVHSEGVRVFPWVAKD
                     RGDVDRCRAADFIDGVMVNDLALFGDACPCGHNPDRT"
     gene            19640..20938
                     /locus_tag="PLW16_01005"
     CDS             19640..20938
                     /locus_tag="PLW16_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPB69513.1"
                     /translation="MYWEKEIETMDRTALERMQVERLTQTLDRASRSPYYAERLKAAG
                     VDARSVRTVADVAKFPLTTKDDLREHWPYGFLAVERDELIRMHSSSGTTGRATVIFHT
                     TGDIAAWTNIVARSMYMTGMRRSDVFQNMMTYGLFTGGLGFHYGAEKIGALVIPAGAG
                     NSKRQIQLMRDFATTVIHIIPSYALHLSTVFQELGVDPRRDTEVKIAFIGAEPHSEKM
                     RQKIESFYGFKAFNSYGLSEMNGPGVAFECPEQNGMHLWEDNYILEIVDPDTLKPLPD
                     GEEGEMVLTTLQREGMPIVRYRTKDLTRVIPGACPCGRTHRRIERIKGRTDDMLILKG
                     VNIFPIQIEKKLMDIPGVGQNFLIVLDREGYNDHMTVKVEVNREFFLGDLKQLEDLRR
                     RIVEDLKSDILITPKVDLVEPGSLPKGEGKAVRVVDNRKD"
     gene            20994..21449
                     /locus_tag="PLW16_01010"
     CDS             20994..21449
                     /locus_tag="PLW16_01010"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB69514.1"
                     /translation="MIAQQISIFAENKPGRLAAVTRILAAEKINIRATNITTADTFGV
                     INFIVDDPKRAAKALTRAGMTVQLRDVLAILLEDKPGGLDRLTQLLAREAVNINNAYG
                     FVLEGSKKAVFVVDVDQMGKTDQLLRKHGIQTLDTEALSSIEPFHYLNY"
     gene            21470..21904
                     /locus_tag="PLW16_01015"
     CDS             21470..21904
                     /locus_tag="PLW16_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69515.1"
                     /translation="MTEKDKGHFADKHRGERSVSPALAAAIRDKAGEGKLPCRRAETI
                     CREMDVTMGEVGKAADLMELEITKCQLGLFGFAPDRKAVKPAAGVSPEFETDIRAACV
                     DGRLPCRSAWDIAARRRIPKMDVSAACEALEIKVKPCQLGAF"
     gene            complement(21986..22705)
                     /locus_tag="PLW16_01020"
     CDS             complement(21986..22705)
                     /locus_tag="PLW16_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HPB69516.1"
                     /translation="MSRIGFISGTALQHLEPFRTLRPKVTETAFGTATVHVSETLAYI
                     RRHGQGPDDYILPHRINHAANLRALKDLGCTEVIGLNSVGSLKMHLSPGTIVVPHDFI
                     ELYGTFTVFSNRPVHVVPGLDEPARRRLIAAAGVAGIEVVASGIYWQTTGPRLETRAE
                     IAMMTAFADMVGMTMAGEAVIACELELPYASLCSIDNYGHGLMATPPAPAQIAATARE
                     NADRMGRIVRTYLKHRPGTPP"
     gene            22919..23257
                     /gene="trxA"
                     /locus_tag="PLW16_01025"
     CDS             22919..23257
                     /gene="trxA"
                     /locus_tag="PLW16_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPB69517.1"
                     /translation="MGKEDLIIHVTDADFDGEVIKADKPALVDFWAPWCGPCKAIAPL
                     VEALAEENRDRLKVAKVNVDDNPNTASAYGIRSIPTLLLFKEGKLVDTLIGMVPKDRL
                     EAFVGKVFQS"
     gene            23254..24042
                     /locus_tag="PLW16_01030"
     CDS             23254..24042
                     /locus_tag="PLW16_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPB69518.1"
                     /translation="MIGRVRSKLPARAGRESADERWRGGEAWKAGLLAALLVLLAGCS
                     WFSHQDMARATPEGLYRQGFEDYQAGRYERAIDNFQRVKEQYPLSPLALLAELGIADA
                     HFSDKNYVEAEAAYMEFAGLHPTNDNLPYVLYQIGMCHYNQRSTIDRDQSETVRAQRE
                     FERLIARFPDSRFAFMAEKKLIDCRRNLAEQEFYVGRFYFRSKKYAAALKRFEGIARD
                     YANLGLDYKVDYFLRETRRRLAEEEARKKAMGLTDTAPAAAPTP"
     gene            complement(24063..25634)
                     /locus_tag="PLW16_01035"
     CDS             complement(24063..25634)
                     /locus_tag="PLW16_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB69519.1"
                     /translation="MTHPQLVAAMSRPEFYPDRPDAVELVQTHISLIFLAGDTVYKVK
                     KAVDFGFLDFTTLAKRRHYCNEELRLNRRLAPQAYLEVVAVTADPNGEPRLGGAGPVV
                     EYAVKMRRLPQERMLKRLLAEGSMRPETMEAVAQKLAAFHREAETGGEIDRIGGIETI
                     RRNHEENFEQTEPFIGRTIPAEQYRFIRAYALGFLERERALFDQRVADHRIRDCHGDL
                     HLEHILVADDILIFDCIEFNERFRYEDVAAEVAFLAMDLDFNGYAVEAVHFVEAYIRC
                     TGDTEIRTLLNFYKCYYAYVRGKVVGFRLDDTAIAAPDRDEAARTAGRYFDLAYTYAA
                     HLERPALILVAGLMGTGKSLLARRLAPLLGATIIRMDVLRKEILALAPTDRRYENFGE
                     GIYSEEISRKTYDTALQRADDLLAAGNSVIIDASYKRDDERRRAEALGRRHGAAFYIL
                     DCLCPEALIRRRLDTRAANAEEPSDGRWDIFEAQRADFDPMSGWASGVHIAVDTGTTP
                     EAMTAEALRGIRQGR"
     gene            complement(25631..26677)
                     /gene="rlmN"
                     /locus_tag="PLW16_01040"
     CDS             complement(25631..26677)
                     /gene="rlmN"
                     /locus_tag="PLW16_01040"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPB69520.1"
                     /translation="MDRPNLRNMTLEELEAFLAGMGKEGYRARQIMRWMHAFGVTDFT
                     AMTTLAKDFRAEIGARARLDLPELVETQVSRDGTKKALFRLSDGERIESVLIPGRNHW
                     TLCVSTQAGCRMGCRFCLTGKLGFRRQLTPAEIAGQLTVLKLRTPEGPDIKNLVMMGM
                     GEPLANYDNTLKAIRILTSDYGHGFSSRKVTVSTCGLAPGIEQLGRDVCVNLAISLNA
                     PDDERRSRLMPVNRKYPLSALLQACRDYPMPGRRMLTFEYILLDGVNDAPADAEALCR
                     LLKGLRCKLNLIAFNEFPGAPFRTPTPERISAFQGVLLRNHYTAILRASKGSDILAAC
                     GQLSGRTVQQEVSP"
     gene            complement(26670..27899)
                     /locus_tag="PLW16_01045"
     CDS             complement(26670..27899)
                     /locus_tag="PLW16_01045"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPB69521.1"
                     /translation="MTLVKKIQVKTTVVEMDGDEMTRIMWRMVKDHLLLPFLEMDLAA
                     YDLHVKHRDDTDDAVTVEAARAIMKYGVGVKCATITPTEDRVKEYGLKKAWKSPNGTI
                     RAMIDGTVFRTPILVRNIRPLVTTWRKPITIGRHAYGDVYSGVELAVPGAGRTEIVFH
                     PSDGGPATTRTIHAFSGPGIVQGTHNTDASIESFAQACFTYALDQKIDLWFSTKDTIS
                     KVYDARFRQIFDREYEARWKDRFRQAGIEYFFTLIDDAVARIIRSEGGVLWALKNYDG
                     DVMSDMVATAFGSLAMMTSVLVSPHGYYEFEAAHGTVQKHYYKHLAGEETSTNSMALI
                     FAWTGGLRKRGELDGTPQVVAFADALEAAALETVEAGVMTGDLLAVADPSPDNRKVST
                     EAFILAVAERLQRKLHG"
     gene            complement(27927..29867)
                     /locus_tag="PLW16_01050"
     CDS             complement(27927..29867)
                     /locus_tag="PLW16_01050"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPB69522.1"
                     /translation="MAATLTQKILESHLAFGRLEAGEEIAIRIDQTLTQDATGTMAYL
                     QFEAMRCARVRTELSVSYIDHNTVQIGFENADDHRYLTSVARKYGLYLSRAGNGICHQ
                     VHLERFGKPGRTLIGSDSHTPTAGGLGLFAIGAGGLDVAQAMAGEPFYLACPRVVRIN
                     LLGALPPWVSAKDIVLRILQHFTTKGNVGCVFEYAGPGIEALTVPERATIANMGAECG
                     VTTSLFPSDGMTRAFLRAQGREKDWIPLAADADAVFERTVDIDLSALVPLAACPHGPD
                     EVCTVRDIGPLAVQQVCIGSCTNASYKDLATVARILRGRTVHPDISFIVAPGSRQVVE
                     NLLRDGYLADLIGAGARLMESACGFCIGNSQSPSSGGVSLRTSNRNFLGRSGTKDARI
                     YLVSPETAAAAVLTGRLTDPRDLDMTYPDVGMPERFYIDDRLIEKPDGVMNPDSVPIL
                     RGPNIGAPPANTPLPEALDGETTLKVGDKITTDHIIPAGARMKYRSNIPRYAKFVFEI
                     LDPTFPARAARIRDEGRHNIIVAGLSYGQGSSREHAAICPMYLGVKAVIAKSFERIHA
                     TNLVNFGILPLLFADEADYDRIAQGDRLTIPNVRAALEGGETLTVENRTKHLTFAVKH
                     TLSTRQRALILAGGALNASCTS"
     gene            complement(30188..31198)
                     /locus_tag="PLW16_01055"
     CDS             complement(30188..31198)
                     /locus_tag="PLW16_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB69523.1"
                     /translation="MMKRRIFPWMGLAVLFSSFILCQTALAKPSYTVKRGDSLSKISR
                     TFHVSIADLKAANDMKGTVLRPGQVLFVPGTQMARLTKSSVSKQKKTASTASRNSKSA
                     RVYVVKRGDSLRKIALKTGVSVKTLARLNHLRTHLIKPGQRLALSAPRLPEESGSSGT
                     QASPDVALRGGDELDAEEDLGPVREEDWAAVEKDKLASSELLGKWNNPSERELLVKVA
                     KGFMGAPYRLGGNSVRGLDCSAFVKKIYSFFDISLPRTAHEQSRVGKHVARGDLVEGD
                     LVFFHTRRPIGHVGIYIGNNEFVHAASGRSKMVRVNSLDEPYYSKHFVKATRLKGLDD
                     GA"
     gene            31452..32351
                     /gene="mtgA"
                     /locus_tag="PLW16_01060"
     CDS             31452..32351
                     /gene="mtgA"
                     /locus_tag="PLW16_01060"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPB69524.1"
                     /translation="MLKWVRIIVLVTVAALAVNVSYYFIFPDVSQLQRKNPQKTAFMK
                     YREAEWREKGHRRKIRQQWVPWSRISPYVAKAVIIAEDDKFWRHEGFDFKAMEKALKK
                     DLQKKRFKVGGSTISQQLAKNLYLSPSKNPVRKVKEAILTWRLENRLSKRRIIELYLN
                     VAEWGEGIFGIEAAARRYFGKPAAALTAREAAQLAAVLPNPRRFKPTGGSRYVTYRSE
                     RIYQIMVRRGIVIPEFEELLTEPEEAATEGWLDLDVPDPLSPPEKDEAPPGAPVETGP
                     QTGDKEQEGLAPPPPSEPAGPSA"
     gene            complement(32431..33681)
                     /locus_tag="PLW16_01065"
     CDS             complement(32431..33681)
                     /locus_tag="PLW16_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69525.1"
                     /translation="MKDVVIVSACRTAIGTFGGSLKGSNSATLASVVMKEAIARAGID
                     PALIEDVRFGCCMEPVDTLNVTRIAALLAGIPETTTAVTVNRVCISAMEAVVSSMAWI
                     QAGLAEIVLAGGVEHMSGAPYSVPAARWGCRLQDQVFVDNLIHGLHAGSHVIRGLEKG
                     PIKEGPIVDLFRGKPYIMGITAELIAQKHNLSREEIDEVALRSHNNAERATLEGDFAA
                     EIVPVEIPQKKGKPPKFFDRDEHFRPGLTLADLAALPPAFIPKIGKVTAGNSSGINDG
                     AAAVLLMSAEKAQALGLKPLARITAVGRGGCHPSVMGLSPVPAVQDLLKRSGMKIDDF
                     DLIELNEAFASQYLGCERELGLNRQITNVNGSGCALGHPVGATGCRIMVTLIHALKKR
                     GKTQGLATLCGGGGVSLAVALEVL"
     gene            complement(33799..33874)
                     /locus_tag="PLW16_01070"
     tRNA            complement(33799..33874)
                     /locus_tag="PLW16_01070"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33839..33841),aa:Asn,seq:gtt)
     gene            34152..35450
                     /locus_tag="PLW16_01075"
     CDS             34152..35450
                     /locus_tag="PLW16_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB69526.1"
                     /translation="MEASEHPHGAQDFKVSRGYAYYVFLLLFLLYLFNYVDRMVVAAI
                     FPYLKADWGLTDTQCGWFASIVTLMMTVFVFPVSLLVDRWSRKKAIGIMAVLWSFGAA
                     ACAFTRNFTQLFTMRSLVGVGEAAYTSGGHAMIAAYFPEEKRATMNGLFTAAIPMGTA
                     IGVILGGVIAETLGWRYAFGILAVPGLIVAILFFWVKDYKTVQIVRTTGDGAQAVRVK
                     MKFADIVREFLHTPSVLFTYLGYVGNTFVTTGLMNWLPSYYHRVDGLPMDKAGLKTSI
                     VFLLAIFGAPIGGYITDRIRKRFLNARMTIPAVTSFLTALFLFIGFSFLEGRAQYLFL
                     IFFGFTAPMFAAGGSAVTQDVVHPGLRAISYSLAQFFMMLLGYSLSPIFIGFISDRYD
                     LLTAFKFLPLFALFGGAAFFVGSFFYVRDLHKVEKVRLEG"
     gene            35507..35785
                     /locus_tag="PLW16_01080"
     CDS             35507..35785
                     /locus_tag="PLW16_01080"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPB69527.1"
                     /translation="MKPNVIVIHPGDNVAVALEDIPAGAAVYLPDGQVFAALTAIPYS
                     HKVALADLVAGAVVVKYGETIGEVTEDVRRGDWIHVHNLDIEEQKENS"
     gene            35786..36943
                     /locus_tag="PLW16_01085"
     CDS             35786..36943
                     /locus_tag="PLW16_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPB69528.1"
                     /translation="METFLGYARPDGSVGIRNHVAVIPSVACANGVVAAIARVLPDVV
                     PLYHGHGCGRAVEIPLHTRVLVNLGRHPNCAAVLVVGLGCEVLKAEGIARAIAETGKP
                     VDYFNIQECGGSRRSATKGIEIAAEMLRAAGRCERRPFPLSRLILGLECGGSDAFSGI
                     TANPAVGLASDWLVDRGGTVLLTEDTEMIGTSPILERRAKTPAVAGEIKTMIADAERL
                     THDLLGPLAPFVIAPGNMDGGMSSIREKSLGCIVKAGSRTITQVTDYGEVPAEKGVVL
                     MRGPGYDTESIAGVAAAGAQFVLFTTGRGNPIGFPTVPVLKVASTAKLYRAMEDDMDI
                     NAGAVLEGKRLAAVGDEIVALIREVADGRKTKAEINRQDGIVCLYTRHPAF"
     gene            36967..37707
                     /locus_tag="PLW16_01090"
     CDS             36967..37707
                     /locus_tag="PLW16_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB69529.1"
                     /translation="MRRVSLFVPCLVDLLLPEIGEAAFALLRRLGVEVVYHEAQTCCG
                     QPAINAGYLPQARKVARHFLKVFGEDEAIVCPAGSCVVTVKHHYPELFADEPDWRTRA
                     ESLAPRVYELSQYLVDVLGVEDVGATFGGKIAYHEACHVLRGLGVSEQPKKLVRAVTG
                     AELVPLNAADVCCGFGGEFSLQYPDISEVLVAEKTENFINSQADALVLCEPGCFLNIS
                     GYVHRHHPEKKVFHLATLLAGNGKGGGA"
     gene            37704..39146
                     /locus_tag="PLW16_01095"
     CDS             37704..39146
                     /locus_tag="PLW16_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HPB69530.1"
                     /translation="MKIRTEQFVEIAREEMKNTRSKAFLGLLPPVINAMRQISLATFP
                     DPDAAQAYGAAIRGEVVTRLPELLEEFERNATARGTKVVWARTAAEANAFVLDLAKKK
                     GVAYVTKGKSMLSEEIGLNDALIDNGVDAIETDLGEFIIQLLKRPPFHLIGPAVNIAV
                     EEIRDLFMEKGILAEPTEDPVALGYAARLYLRDKFHRLEMGVTGVNMAVAETGTIVNV
                     ENEGNIRFAKSSPRTQVSIMSLEKVVPTMADALHMIRLLCRNGTGQKMASYISFDTGP
                     RAAGEIDGPEEVYCVIVDNGRADLYDDPVARDVFRCIRCGACANICPVYTKIGGYPYG
                     WAYTGPMGQLLTPLLLGFSGTSDLFRACTLCGVCKSVCPAGVDHPRMLLYYRARDAAG
                     DPRLRSRGRPLKERTLYDLFAWGATHPRLWRQGAKRMRPRVNRGARDGYLSRSVEPLD
                     GWFRSRDLPEMAEKTFHERWKEREGHKAKA"
     gene            39170..39844
                     /locus_tag="PLW16_01100"
     CDS             39170..39844
                     /locus_tag="PLW16_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPB69531.1"
                     /translation="MPYAAREDILGKLKKADRREEAPARPPLPPLTEVGWGTEQLIEK
                     FTENLALQTGVVHRVADRAGALEKLTEIAQAEGWKAVMIATDEVLAPLDLAAWGRTNG
                     IDVLKAADFTARDAFKDAVFDQVDAGITGVDWAVAETGTVALVHDERQPRLVSLAPIL
                     HVVVVPVDRILPVYEQALERILAARGGMPAHLNLITGPSMTADIQATPFKGMHGPRRV
                     IALLIG"
     gene            39925..41172
                     /locus_tag="PLW16_01105"
     CDS             39925..41172
                     /locus_tag="PLW16_01105"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPB69532.1"
                     /translation="MKSVWVPSRRWYENEERELTFPDRWGVDNLTSPGLEKPGLTPEE
                     VAAKIARPVAGPLLEELARGKKQAVIVFDDMTRPTPVKEVATTILDALRRAGMQRDQI
                     RFLWALGSHAAYDMIAARKKLGDDIVERYAVYNHDAFQNCVRVGRTPTGVELWFNREY
                     LACDLKIGIGCITAHVHVGFGGGAKIVLPGVAGIETINQFHNQQFRDFARTGLGNFDN
                     NIMRAECDAAGDLAGLDFKVDCLINRRGEIANLYAGPFRETHALGAEEGRVHYGIPCA
                     VGYDVTVCNAYGKANESAIALVFTLATMKPGQGTGVLVCDAPEGQVPHYVMRAWGSDY
                     GGRHYQARAKGFVGLLMKKLIVFNPQPDRTSLDLVCHADDAVVVKTWDEVRSILEADY
                     PGEAKVAVVQDGTMQYILPPGNG"
     gene            41236..42366
                     /locus_tag="PLW16_01110"
     CDS             41236..42366
                     /locus_tag="PLW16_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPB69533.1"
                     /translation="MEMTIMGQKELQERVHRARLCTGCGACVNLCPYQVFYNDQTIVL
                     DACDIKEGRCFAFCPRTPTDLDAMRRRFFAPGNLTPEIGAVKGYLIARAADAAVRRGA
                     QHGGTVSALMALALDEGLIDTAIVAEGADRFLQQGVAVGDGAEVLKRGKSKFIAAATV
                     AAFNRAAKGPAEAIGVVATPCQVFALAKMRMKPLPELDNNIDKLKLVIGLFCGWALDW
                     RKFTALLAARTALEDITGMDIPQGRRLVVVYTRNGALEIPWDEVAPIAREACGVCIDT
                     TAEFADLSVGSARIGETWETTRTWNQVIVRTEAGQALIDLARRKGVLEFRDPPAGVLD
                     ELKAAARDKKKASLEKIIARTGNPEDLIYVDRRDPVIRAILD"
     gene            42400..44334
                     /locus_tag="PLW16_01115"
     CDS             42400..44334
                     /locus_tag="PLW16_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB69534.1"
                     /translation="MSKLIDPREGVRVLLMGNEAIVRGALEAGVNVAAGYPGTPSSEI
                     VQNLADVAGERNLYVEWSVNEKVAMEVAAAASFAKLRSLCAMKQNGVNVASDFLLHLT
                     AAGTRGGMVLVTCDDPGALSSVNEGESRHFARMVEVPLLEPGDFQEAKDMTKWAFELS
                     EEIRNLVVLRSVTRMSHASGNVTLGRLSDARPKAEFKYNGFILDPLEGPVVSAPVPYK
                     HTLQQEKLKRVREIFEESPFNTYAGPEKPDLLVVTSSACTLYSREAIDVLGVKDRVGL
                     FKLGTTWPLPPELLKKRLASTDQVLIVEEVIPFLEENVKILAAETAREIGVKTFFGKN
                     DGTLPSIGELNPDLVAVAIAKILGIDHVAMPPEYEKKATLMSFAKAPNRELTFCPGCP
                     HRASFWAIHHALLMDNRLGFVCGDIGCYSMAALPCGFSTLKTLHAMGSGTGVASGFGQ
                     LHRFGMEQPAMTVCGDSTFFASVMPALVNAIHHRAELTVVVLDNSGTAMTGFQPHPGL
                     KVNAMGGDVPALDIARICAAMGAKVEVRDPFQIEETRETLIRLLDQKGVKVLIMRQMC
                     ALSPEKRAKKNYEVKVDEALCLGETCGCNRLCTRVFRCPALVWDKERKKARVDEVLCA
                     GCGVCALICPAGAIRKEEVA"
     gene            44338..44964
                     /locus_tag="PLW16_01120"
     CDS             44338..44964
                     /locus_tag="PLW16_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPB69535.1"
                     /translation="MGTQTLPNDPFNIIITGVGGQGNVLASRVLANMLVRRGYDVTIG
                     ETFGASQRGGSVMSHIRVSTRGAWSPQIPKGKADMVVSLEPVEAIRVMKDYGNEEVRI
                     LVNDRPIYPVGVIAGELNYPALDEIAGALKDLSKHVWFIDATEEAIRLGNPILGNVIM
                     IGAACGLGILPVGRAEFAEVMKESLAADKLEINLAAFDLGAAKPAAAA"
     gene            45011..47374
                     /locus_tag="PLW16_01125"
     CDS             45011..47374
                     /locus_tag="PLW16_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB69536.1"
                     /translation="MAKLTLKEWLARVEGDRRFMENVRAIRRIPARDGVYAPYPDWVH
                     PSLAKVLAARGMGRLYSHQAEAVACVRRGESVVVVTPTASGKTLCYNLPVLQRILEEP
                     ETRALYLFPTKALAQDQMHEVHGLIGGLGADIKTYTYDGDTPDDARRAIRRQGHVVVT
                     NPDMLHAGILPHHTKWQKLFANLRYVVIDELHIYRGVFGSHLTNVLRRLVRIARFYGA
                     DPVFVCCSATVANPREHAERLLERPVALIDESGAPAAAKTFILYNPPIVNRELGIRQS
                     ALTPARKMAGDLIENGVQTIVFATSRLNVEVLTKYLKDRFAKGKPVDAQFVTGYRGGY
                     LPNLRRQIEAGLRSREVMGVVATNALELGIDIGDLEAAILCGYPGSIASTWQQAGRAG
                     RRTGLSVAVLIAKSAPMDQFIVENPDYFFSRSPEFCRVNPDNLLILLHHIKSAAFELP
                     FEKGENFGGENLEELLDYLEEKGVLHRTGERWHWSAESYPADEVSLRSVNPENVVVVD
                     ATEAGNHRVIAEVDWDSAFTTVHDDAIYMVESQQYYVDKLDLERKTAYVHKVEAEYFT
                     DAMTYTNVRVIDAFAKKPGREVLVEHGEVQVVSKVVGYKKIKFYTSENLGYGDVNLPE
                     RDMHTTSYWFTIPRDRLSRLPFHQAEIIDGLAGTAYALHHLAAMLLMADLHDLDRAIG
                     DKSGEWFVRTGKQGRTITASPAGEDGATPTMDAFDPTIFLFDAYPGGIGFSDLLFERH
                     DLLMRLARQLIESCPCPNGCPSCVGPVLEVGPKAKEAALAILDLIET"
     gene            47656..47964
                     /locus_tag="PLW16_01130"
     CDS             47656..47964
                     /locus_tag="PLW16_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69537.1"
                     /translation="MNIHILETTNNFIKVQENVWESGYWKLDEAQAKKLVGGEIYFHK
                     KRQEPSFYGGTILAYRVAPEGVHEGRIIFTLKHLTACRNVRTDKSGWSKNMKVIDPES
                     "
     gene            48140..49456
                     /locus_tag="PLW16_01135"
     CDS             48140..49456
                     /locus_tag="PLW16_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPB69538.1"
                     /translation="MSVRGRLQRLTGETTNGGNDSREGSRAEDISALRERIEAILSRR
                     PEAGQGGVSPSFRGRGVALEDLVPGVETANAAGVFFCAYQKAGESVRHGARCVRDWLS
                     LDMERLAVLANDAALNRLEVAQALFLDTETTGLAGGAGTVPFLIGLGWFEEGGFATRQ
                     LFARDYAEERAALVHLRELAAERRFLVTFNGKAFDVNLLAARFIMNRLPDPLSGLPHL
                     DLLHPARRLLSHRLGNRRLGALEAEILGFEREGDIPGSEIPRRYFDWLRNRDGRLMAD
                     VFTHNRLDILSMAALAARLTDMIVSDGATSADRPDDRLAAARFLLARDRPETAARMLA
                     PLCACDHPDTAREAARELSLLHKRAGHWTAAVRLWEEMARRDPGDAFPLVELAKWWEH
                     RRHDFDRALALTDRAGAGRLSGDDRAALDRRRDRLNRKQAARPARA"
     gene            49636..50694
                     /gene="waaF"
                     /locus_tag="PLW16_01140"
     CDS             49636..50694
                     /gene="waaF"
                     /locus_tag="PLW16_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPB69539.1"
                     /translation="MPGNLASVNSILVRCPNWVGDIVMATPVFSCLRENFPEASIVAC
                     IRGYARGIIEDGPWFDGIIACNDKTFAGFRQTVSEIRRIRPDMVILLPNSIRSLLTSW
                     LGGAKKIYGYKRNLRGLLLAGGPAPVRDGAGRVKALPMQTYYLEICRSLDLTPPDDPR
                     PELFVSAELQAKGESLLRKYGIAPGDRVIGLNPGASFGSSKCWPAEYFAELAGLLKRR
                     LDGKIMLFVGPGEEGIAEAIVKHCRVSVIDTGPDRIDLAALKPLIQRCNLLVTNDTGP
                     RHIAVAFGVPVVVIMGPTDPRYTSANLDKSVVIRKTLDCSPCHKKVCPWDHRCMTEIR
                     PEEVLGEAIKLLERSDLR"
     gene            50691..52175
                     /locus_tag="PLW16_01145"
     CDS             50691..52175
                     /locus_tag="PLW16_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPB69540.1"
                     /translation="MRPEFYEGKWARLRERPTAEALKPLAKQIALSFLDEYFYNDRYE
                     GGYIRLLCEMATAFEDDALNLSGAGALFGIVIESLCDDFEELQTETYNRVMCQVVTHC
                     RTLPAGAWLDRRLRDFGMDSYESLFGRIETFRNHSDAFRPDIRPPKKILLLSRVTIGA
                     DVAITSVMIQRLKRTFPEAEIRVIGGAKLDQIFGGNPDVHIDVFDYSRRGGLFERFRG
                     WEAVLEGIGREAPPDLAREVILVDPDSRLSQLGVLPLIGDDQYLFFNSRGKDSYPKKM
                     SISELTNHWLDRVFGEGEFRFPRVWLQKDDLDRAARFVEGLRAAGCGRVIAINFGVGG
                     NSRKRLSEAFERRLIATLLEEPRTVVLLDKGFGEEELGRTARLMAAFADRPLLDIPFA
                     ALPDRAMTGGLAGVECDIGEIAALISHSDEFIGYDSACQHIAAAVGVPTYTVFAGSNN
                     TRFIRRWHACGPVRSEIIHVDTLTHPPMFDDEDVVARIIDARRV"
     gene            52202..53716
                     /locus_tag="PLW16_01150"
     CDS             52202..53716
                     /locus_tag="PLW16_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB69541.1"
                     /translation="MKVLFVYPNVGSQLGFNYGVSYLSALLKAAGHEVVFRQMCEDLG
                     PLPSKDEFIAWLKEIDPDVIGFSVVTNQWPYARELAGWCREATSAPLVCGGIHATVAA
                     REVLATGAFDYVFSGECEEAFPEFMDRLARQEDVSDLRNLGHVVNGEIRINPIRPLPD
                     LESLPPKDYRIFDFQRIIDAKNGWVGLMASRGCPFNCTYCFNHVMVKKYRDDLACSFQ
                     DLHYIRHHRVEQMIDEIVFLQKTYKNIKMFLFDDDLFTFDRDYVIRFCRAYRKVSSLP
                     FVVNAHVGFFDEERARALAEANCKIVKVGIESGSEHIRRQVMNRRTSNRRIVEVIRLI
                     DRYRMHSSCFIMLGLPYEGRREVMETVTLLGEARPGRFRWTFFFPFPGTKAHDLSVQG
                     GYVNFDRMGSLMNFTDESCLDFGPEHNLFLKKVGLILPWFVNAHARLEVSPFYRGRVD
                     ALLKMDLETFEMAAPSIREEDREISSRFQAEGQTHYAIKYNPFMGVISDYFTQE"
     gene            complement(53880..54575)
                     /gene="aat"
                     /locus_tag="PLW16_01155"
     CDS             complement(53880..54575)
                     /gene="aat"
                     /locus_tag="PLW16_01155"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPB69542.1"
                     /translation="MPVFELTEDILFPPVRLADRSGLLAVGGDLSPARLLAAYRQGIF
                     PWYGEEDPLLWWSPHPRFVLFPRELHISGSLRALLRKNAFTITFDRDFPAVIEACGRP
                     SPGRDETWITPEMREAYLTLHELGYAHSVEARQGGNIVGGLYGVSLGRCFFGESMFTE
                     VSNASKAAFVTLVERLGARDFKLIDCQVFTRHLQSLGARMIARKTFMNRLRLALAYPT
                     LRGNWGERPDFIV"
     gene            54680..55222
                     /locus_tag="PLW16_01160"
     CDS             54680..55222
                     /locus_tag="PLW16_01160"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPB69543.1"
                     /translation="MNLFGKPKGKKLHTRTIEVNTYEYGAGRLVVEGALKDLRWQDHY
                     LATGEKKPPGVLHGMIIHLLVNVRNLEIEDVHVEMPDTPREECLETRGCIARVKGMRI
                     AGGFTLKVKEMLGGIQGCSHLLALLTAMAPAAVQGFAAHILRDDTELKSTWAGLSRFL
                     EDTCWVWRKDGPLLKKLQSL"
     gene            complement(55272..57533)
                     /gene="clpA"
                     /locus_tag="PLW16_01165"
     CDS             complement(55272..57533)
                     /gene="clpA"
                     /locus_tag="PLW16_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HPB69544.1"
                     /translation="MKISETLNRIIMAAYAEANVRHHEFITPEHLLYACLFFEDGRDI
                     IGHCGGNIEHLKRLLARHLQETHTPVEGNQAFQSLGFQSVLERAVWHTSNAQKDILEL
                     GDVLVSLLDEKESFAAHFLRKEGISRLSLLNYISHGVSALPPEGESGPRPDTPPARED
                     AKPAEQKEPDAGKILRAFTTELTAKASAGEFDPLIGREEILDRTLQVLCRRLKNNPVH
                     VGEPGVGKTALTEGLAQRVAAGAVPKKIRDAKIFALDMGALLAGTRYRGDFEERMKRV
                     IAELQKMENVILFIDEIHTIVGAGAVSGGSLDASNILKPALASGNLRCIGSTTYDEYR
                     KHFEKDGALSRRFQKIEIPEPTAAETLEILKGVKTRYESYHGVVYTDAALQAAAELSH
                     RYINDRRLPDKAIDVLDEAGAVIGMEAGEDSDVREIDRRTVEAVVAKIARIPERSVSS
                     SEVEKLKDLEAELKRRIFGQDAAVETVVEAVKRARAGFREADKPVASLLFVGPTGVGK
                     TELARQLADVLGVPLHRYDMSEYQERHAVAKLVGAPPGYVGYEEGGLLTEAIRKTPHA
                     VLLLDEIEKAHTDIFNALLQVMDYATLSDNTGRKADFRNVVILMTSNAGARDIGRPVI
                     GFEGRPLNRDAVFPAIEKTFSPEFRNRLDGVVNFNALTDTVVLDIVRKRIDEFRLQLK
                     GKGVTLEVTDACTDWLVRRGSSPEFGAREIGRLIQDKIKHFFVDEVLFGRLREGGAAR
                     ADIEDDDVVVRSV"
     gene            complement(57539..57859)
                     /locus_tag="PLW16_01170"
     CDS             complement(57539..57859)
                     /locus_tag="PLW16_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPB69545.1"
                     /translation="MPQSLHPDRPAQHPDVHDDVAHQEPKMYRVVLHNDHYTTMQFVV
                     EVLIAIFHKAAADATRIMLDVHRKGRGTCGVFTYDIAMTKIDQVHQLAQKHEFPLKCS
                     AEEA"
     gene            58078..58206
                     /locus_tag="PLW16_01175"
     CDS             58078..58206
                     /locus_tag="PLW16_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69546.1"
                     /translation="MEQKEIIDFTIAHGASQAAWDAFLSDLDDRRGAYLFYGQADS"
     gene            58268..58984
                     /locus_tag="PLW16_01180"
     CDS             58268..58984
                     /locus_tag="PLW16_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69547.1"
                     /translation="MEKADNVWDFFDKFYCISLKEREDRRRSAREEFSKVGLADRVEF
                     VLGERLPYDLEQQVYESHMACLRKGLESGAQSIVIFEDDVEFDRFDTKHLINCINFLR
                     QHPDWKVLLFGALIRSSCKTDNPCVQKVRYQSLTHAYALNRHYAQELAYEPWEGIVND
                     TLFRSLTDGIYALYPMCAFQKDFKSDNYKYPRLEKIRRLLGGLNRIQKGFEFYSRHKF
                     GIYAVQTAAILLVLFAFIGR"
     gene            complement(59264..60157)
                     /locus_tag="PLW16_01185"
     CDS             complement(59264..60157)
                     /locus_tag="PLW16_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPB69548.1"
                     /translation="MGRTDLYFAYGTNMNRADLQTWCDRKGLPYPLGRKVGNAWLPDM
                     RQVFNFLSERRGGGVSNLRHRTGQAIPGVLYETLPDGWATLDAREDAPKVYKRLPVVV
                     LTGDGREYSAVTYQVDPAMIEGIFVPPHPDYIAAVQEALIAHGLDDRMFNAVSLGFDP
                     PWTVDHLFVYGTLKEGGPRHHILETWGDHTCRGTAQAPGLLFDSGKNFPCMTPPEGED
                     AWVAGEVYPLKDPRKAFEMFDIVEGFKGYGNPGSRFRRTLTRVRTLDDSCFTAWVFMY
                     AGSTAGMSRITSGSWKGPPPA"
     gene            60466..61218
                     /locus_tag="PLW16_01190"
     CDS             60466..61218
                     /locus_tag="PLW16_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HPB69549.1"
                     /translation="MRAAKTPPGQTLAGRKDAGRRRVFWAFLGLVLTVLPAYAVTPEE
                     ALRKDFPELKFDRIGPSPVPGMAEVIVGPDILYYHPREGVIMTGEMISRELKNLTRQR
                     KGELQAEQIKTLPLDLAVRIGTGSHRVIEFTDPNCPYCRQASKYLKDRMDLSRYIFFF
                     PLSPRSEDKIRYVLCAPDRGKAYEDVMAGRRDGVEPEICKNSQAGEIQKRHRDLGQRL
                     GLEGTPFFVIDGEVVAGADLPRMEKLLEAKKP"
     gene            61215..62141
                     /locus_tag="PLW16_01195"
     CDS             61215..62141
                     /locus_tag="PLW16_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPB69550.1"
                     /translation="MKLRREAYLRPLTVLLAFAGVGVALYYLTCDDACRYLSGSVFRV
                     DLKYWGIGYMGGVLVLGLFRRTLWNSLLLSFGVGGEVFLVGFQVVHRVFCPYCLTFAA
                     IVLLLFALHVRPGKKAALLLFAMAGLLTFLLAFSGSATPVYAAGPAHVPTFGKGPVNV
                     RLYTDYACEPCQMLETRAAPLLDALVRKDKIRLTFIDTPIHTETKLYVKYYLYVLRES
                     QTLASAFRIRTLLVEAAQKRVRDPTKLEAFLVARGVSVRPVDVTPTLEVFLGYLSKDR
                     IQFTPTCVVESRAGKKTYTGVNDILAALKRIR"
     gene            62377..63591
                     /locus_tag="PLW16_01200"
     CDS             62377..63591
                     /locus_tag="PLW16_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPB69551.1"
                     /translation="MKDVFQAVQVTDHVYWVGAIDWDIRDFHGYTTPRGSTYNAYLIL
                     GDQIALLDTVKAPFMGEMMSRIASVVDPQKISVLISNHSELDHSGGLPAAVEAIRPGK
                     VYASAVGVRTLQELFHGALEVLPLKDGDRLSLGNLNLTFLETRMLHWPDSMFSYLEEE
                     ALLFSQDAFGMHLASLDRFDDEMDPDVLKHEAATYFANILLPYAPLVTQLLARVGNLG
                     LRLGIVAPDHGPIWRKDIPVILDAYGRWARQEPTRKAVVVYATMWHSTERMARALSEG
                     LKAEGAEVRVMAMDHVHRSQVAYELLEAGALICGSPTLNNHLLPQMADVMTYLKGLKP
                     RNLMGAAFGSYGWSGESVRQLNDILVEMKVEKVGEGIRVKHRPDAGVLADCYALGREI
                     GERLRAKAAPAA"
     gene            63618..63776
                     /locus_tag="PLW16_01205"
     CDS             63618..63776
                     /locus_tag="PLW16_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPB69552.1"
                     /translation="MKSYECGVCGYVYDPAKGDPDNGIAPGVPFEDLPEGWTCPICGA
                     EKDQFSPL"
     gene            63787..64281
                     /locus_tag="PLW16_01210"
     CDS             63787..64281
                     /locus_tag="PLW16_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB69553.1"
                     /translation="MATIRDALYQAYTGEAKAALRLKVYAEKAAEEGYLQMAKLFRVI
                     AYSEEIHGARALRVLREIKSTEENLADSFESETRVAEVAYEQFIKQAEAEGNKPALLH
                     FSQSRDVEETHAKLYKEAMTHFLEERETHYHVCKICGYVADGVLPEECPVCGAKRDHF
                     VSFD"
     gene            64354..67152
                     /gene="ileS"
                     /locus_tag="PLW16_01215"
     CDS             64354..67152
                     /gene="ileS"
                     /locus_tag="PLW16_01215"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPB69554.1"
                     /translation="MNYKNTLNLPQTPFPMKANLSQKEPDMLRRWEEMRIYDKIREAS
                     QGRPVYILHDGPPYANGNIHLGTAMNKVIKDMVIKAKNMSGLDCLYVPGWDCHGLPIE
                     HQVDKELGERKEGLSQADKRRACRVYAERFVDVQRQQFKRLGGFGEWENPYLTMHYEY
                     EAITVAEFCKLFLSGDVYKGKKPVYWCATCKTALAEAEVEYADHTTPSIYVKFPMISD
                     VAAVRPKLAGETVSVVIWTTTPWTIPANLAIAFHKDFEYVALKIRGEVLLLAKELIDY
                     CLDAFGFRGEAYEILDEFPGAAVEGLKCRHPLIDRESLLILAPFVTLEAGTGCVHIAP
                     GHGQEDYEIGVVYGLDNYAPVDADGKFTPDVKDFAGQFVFDANDAVNDKLREAGTLLG
                     RVDIDHSYPHCWRCKEPIIFRSTEQWFISMDKNGLRKKALEAIDRVQWIPAWGRDRIY
                     GMVENRPDWCISRQRLWGVPITIFYCRHCQQELLTTEIADRIVALVREHGADVWFERT
                     AKELMPAGTVCPSCGGDAFTKETNILDVWFDSGVSHAAVLETRQRHRSPADLYLEGSD
                     QHRGWFHSSLLESVGTRGQAPYRSVLTHGFVVDGDGKKMSKSVGNVVDPQEIIDRYGA
                     EILRLWVAAEDYTADIRISEEILKRLVEAYRRIRNTGRFILGNLYDFNPKRDRVAPTE
                     MMDIDRWALLRLKEIIRRVRDAYERYQFHVAFYTLYNFCTVDLSALYLDVLKDRLYTS
                     PAASLERRSAQTAMMTILDAMTRLLAPILTFTAEELWGALPPFDGKASSVHLTQFPEP
                     QTDPDDAALGERWKTLIALKGEIAKAVEIARQNKVVGHSLDAAVALTLPEMLRAFVEG
                     AREELKTLSIVSRLVVVEPGAMDAAPYASEEFPGLMIGVSKAPGEKCNRCWMFSETVG
                     SRPAHPTLCERCARSLEP"
     gene            67164..67649
                     /gene="lspA"
                     /locus_tag="PLW16_01220"
     CDS             67164..67649
                     /gene="lspA"
                     /locus_tag="PLW16_01220"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPB69555.1"
                     /translation="MPRKYVVLVITTLVVVFLDQGTKAYIAGTLPLHQSWEVLPGLFN
                     ITHIRNPGAAFGFLSGASPRFRILFFIAVTLLALGLILHYIYRATAGEGFQAACLSLI
                     FAGAAGNLIDRVRFGEVVDFLDVHWGVHHWPAFNVADAAISVGAILLILALIREGRSK
                     T"
     gene            67729..68601
                     /locus_tag="PLW16_01225"
     CDS             67729..68601
                     /locus_tag="PLW16_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPB69556.1"
                     /translation="MNRLRLVFILLILGGGLGFLFIPAAVPPDSVFLPIMPPSRADAW
                     NAPAEDHCGLRVVRAYPHDPEAFTQGLFYEGGNLYEGTGLYGKSSLRRLDLVSGRIVR
                     IRRLSPAFFGEGVTSCRNRIIQLTWRERVGFVYDLQTFRLLGRFPREGEGWGITCDGR
                     RLIVSDGTDRLQLLDPDTYRPVGSLSVRDREGRSVARLNELEFVEGEIFANIWQSDRV
                     ARICPKTGRVAGWIHLPELERGPAGEEPNGIAYDARGKRLFITGKLWPHVYEVILTPL
                     VGPPRTKETGGAAP"
     gene            complement(68546..70252)
                     /locus_tag="PLW16_01230"
     CDS             complement(68546..70252)
                     /locus_tag="PLW16_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPB69557.1"
                     /translation="MGRSRTRIFLGVALFVVFTGATGPLQAQAPSDSPPLSPTPFSTI
                     DRTFQGPEEEALYESALVRQFYENRAWRPAWTDAAGVSPAAMDFLDTLRRVDTEGLFP
                     SDYHLQRLETLSARLSPSASAPIGQAPENRSRFEWLMTDTFLHCGRHLSNGRTGYARG
                     DAPGANLCPDTDLADTLEKALAEGRVGEALLDFSPRHGYFLRLRAALSRYRGIVARGG
                     WPVIPPGPTLRRGTQGPEVSLIRQRLAAEEGLGEESTATPEDFDTRLEEDVKRFQQRH
                     GLKASGAVDADTRQAMSVPAADRLALIELNLERWRWLCRQLGDDYLLVNIPDFRLRAV
                     RDGFPVLELRVVVGQTRPEWQTPEFNSLLSQIVFHPFWVVPRSIATREMLPKIKADPD
                     YLKRQRLQVVRSARDKTGLDPFEIAWESLSVHHFPYTLRQDPGAGNALGQIKFVLTNT
                     PDIYLHDTPSKRHFRRHVRTFSHGCVRVEKPLALVRHLFGDTPATEKLIREGLMKKRP
                     TTINLPQPVLVYLVYVTSWAEADGNVHFRPDVYGRDPLLSALLREPRHPFPSFEAAPP
                     EG"
     gene            complement(70381..71682)
                     /locus_tag="PLW16_01235"
     CDS             complement(70381..71682)
                     /locus_tag="PLW16_01235"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPB69558.1"
                     /translation="MTPITVGIVGFLILLVLMFLRMPVGFVMAVVGILGFGQLVSWPA
                     AFNLAARDFFATFSAYNLTVIPLFVLMGQIAFHAGISGRLFNAAHKFVGHLPGGLAMA
                     TIGACAAFSAICGSTSATAATMASIALPEMKKYRYDPALATGVVAAGGSLGILIPPST
                     IFIVYGIMTEQSIGKLFMAGVLPGLLLTGLFIATILIWTRLQPQIGPRGPRTTAAEKL
                     RSLAGLIETLLLFILVMGGLFAGIFTPTEAAAIGAFGTLLIAVIGRNLSWRGFLRAMN
                     DTTRISCMIFVIVAGATVFGHFLAVTGIPSGIGEWVSGLHLHRMLIAALVLVVYILLG
                     CLMDSLAMIMLTIPVVFPVATALGFDPVWFGVIIVLAAEMGVITPPVGINVYVVAGVA
                     RDVPLHVIFKGTTHLLAALVVTALLILAFPQIALYLPGLMK"
     gene            complement(71679..72164)
                     /locus_tag="PLW16_01240"
     CDS             complement(71679..72164)
                     /locus_tag="PLW16_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB69559.1"
                     /translation="MDRPDRIIRQASHLFSHMASGAVVTMMLLSTADIVLRFFGRPIP
                     GAYELVGFLGTLVVSFALARTAMEKGNIAVDILVEKLPRRAQTVIGLANDAIGGVLFG
                     LIGWQSLRYALELKQSGEVSSTLGMPVYPFALGIALGCGLLALVLFSEFLARFDKAVR
                     S"
     gene            complement(72261..73334)
                     /locus_tag="PLW16_01245"
     CDS             complement(72261..73334)
                     /locus_tag="PLW16_01245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB69560.1"
                     /translation="MKRKCCTLLLIVSAVLLWMVNVTAIDCRAAEITLTYSIFFPSTH
                     GQAKAADAWAKEIEKRTQGRVKINLFYGGTLTQADQCFDGVLNGISDLGMSCFAYTRG
                     RFPVMEALDLPLGYPSGQAATFVANAFYRRWQPRELSGVKLLYIHAHGPGLLHAVKPI
                     RTLEELKGKKIRSTGLSAKVVTALGAVPVAMPQGGTYEALQKGIVHGTFAPIETLKGW
                     KQAEVVKSTTESTAIGYTTAMFVAMNLKKWQSLPPDIQKVLEQVSEEWIDVHARAWDN
                     LDAEGRRYTLSLGNRIIPLSQAESRRWVRGVKPVVSEYIDANRTQGVNPEKAVRDLQA
                     SIKAFGKKKFSSYRQYKQSTGVK"
     gene            complement(73648..73950)
                     /locus_tag="PLW16_01250"
     CDS             complement(73648..73950)
                     /locus_tag="PLW16_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69561.1"
                     /translation="MPLNSLMLGGAVAVVLGLVGLIGWHEAFFILLKGGLPIALLLGG
                     ILAVYVGFDDLQDKIREEKRRHEEKLDQAREEIELAKARAELYREELEKLKDGNQK"
     gene            74335..74715
                     /gene="hisI"
                     /locus_tag="PLW16_01255"
     CDS             74335..74715
                     /gene="hisI"
                     /locus_tag="PLW16_01255"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPB69562.1"
                     /translation="MTEPDFDKGDGLIPVIVQESETGEVLMLAYMNAEAWRETLRTGK
                     ATYWSRSRKTLWVKGESSGHVQWVKEIRIDCDRDTLLLRVRQVGDAACHTGYRSCFYR
                     KLSPGSEAVVGERVFDPETVYNKG"
     gene            74816..75736
                     /gene="hisG"
                     /locus_tag="PLW16_01260"
     CDS             74816..75736
                     /gene="hisG"
                     /locus_tag="PLW16_01260"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPB69563.1"
                     /translation="MAPKKKTQAAPVCGTPGRRLKIGIPKGSLENSTIDLFKRAGWRI
                     TYDSRSYFPDIDDEELFSTLVRAQEMSRYVEDGTLDLGLTGRDWILENESRVVVVQDL
                     VYSKASMRPARWVLVVREDSPIRSLADLEGKRISTELVNFTKKYFRKQKINVQVEFSW
                     GATEAKVVEGLVDAIVEVTETGSTIRANKLRIVQELLKTNTQLIANAEAWKDPWKKQK
                     IEQIRTLLMGSLQAMNKVGIKLNVARKNLGRVMLLLPSLKAPTVSSLYSEDWFAVETI
                     VDMAIVRELIPLLKEAGAEGIIEYPLYKVI"
     gene            75744..76331
                     /gene="hisB"
                     /locus_tag="PLW16_01265"
     CDS             75744..76331
                     /gene="hisB"
                     /locus_tag="PLW16_01265"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPB69564.1"
                     /translation="MKRKARIHRKTSETDIAITIVIEGTGVARVNTTIPFLDHMLALF
                     AKHGRFDLDVQASGDTEVDDHHLVEDVGICLGNALKEALGGKWGINRYGNALTPMDET
                     LCSVALDLSGRPYLVYHVKFGNRKIKQFDAALLHEFFKALTDRSGMTLHINLVYGKNT
                     HHIAEGTFKAFARALMEAVAIPEGRTDLPSTKGLL"
     gene            76395..77081
                     /locus_tag="PLW16_01270"
     CDS             76395..77081
                     /locus_tag="PLW16_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69565.1"
                     /translation="MTPLGKACLLMAIVVALWGTAACASRPGVAAGVPLKETAVLKRI
                     AVLPFAEIAPDETSGRSGRCPVCGALFSGEAMPEGAAGVVESAFLRRIEAGGKFDLVP
                     SDRTQAVYRRLEAAALKQTTLQILRAVGRDLAVEGIVAGYVYRLRERKGYPYAAERPA
                     SVAFEIHLIRVSDGASVWRGIYDHTQASLMENILHAASFYREKGRWVSAAELTDEGME
                     GVLQNFPGLN"
     gene            77160..77912
                     /gene="hisA"
                     /locus_tag="PLW16_01275"
     CDS             77160..77912
                     /gene="hisA"
                     /locus_tag="PLW16_01275"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPB69566.1"
                     /translation="MILIPAIDLKDGQCVRLRQGDFSRVTVYGEDPAAMATHWCHQGA
                     TRIHVVDLDGSRAGEPRNRRAVQDIVRTVPIPVEVGGGIRTLDTIADYLDLGVRWVIL
                     GTAAMKNAALLEEACRRWPGRIILGLDAVDGRVAVEAWTESTAETAIELARRYEGLAL
                     AAVVYTDIHRDGMEVGVNVPATKLLAESLRLPVIASGGVAGIADIEKLLEIENAGVMG
                     VIIGKALYTGAISLPEALRLREGSTPAASHGG"
     gene            77917..78261
                     /locus_tag="PLW16_01280"
     CDS             77917..78261
                     /locus_tag="PLW16_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPB69567.1"
                     /translation="MEDCVFCKIISGTIPAGKVYEDEHVLAFDDIHPMSPIHVVLIPK
                     RHVPTLLDLAPSEDDLPRHLIQGVQAVARIKGLDRNGFRMVINCMEDGGQVVFHLHCH
                     ILGGQRLKDDLA"
     gene            78331..78780
                     /locus_tag="PLW16_01285"
     CDS             78331..78780
                     /locus_tag="PLW16_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPB69568.1"
                     /translation="MNFREKMTEDMVQAAKHREKIRLSTLRLIKTALHNREIDLKREM
                     NEAEFLQMLASMVKQRRDSIEQFGKGGRADLVAQEEEELRIIQEFMPRPLSDEEIERE
                     IDLAIDEAGAAGPKDLGKVMKILMPKLAGKADGKTVGEKVKAKLTGA"
     gene            78797..80593
                     /gene="dnaG"
                     /locus_tag="PLW16_01290"
     CDS             78797..80593
                     /gene="dnaG"
                     /locus_tag="PLW16_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB69569.1"
                     /translation="MKGSIPADKISEIRDRTDLVALVSEYVTLKKAGRNYLGLCPFHQ
                     EKTPSFTVSPEKQMFYCFGCGEGGNGFDFLMKASHMTFPEAVRHLAAKTGVVIPEPVR
                     SAKEKAQDGLREQAVRVNGLAATHFARNLHGAAGRVARAYLENRGISEETIQVFRLGY
                     APDAWRSLRDYLARSGAPVDLAEKLGLIIPGKDGGFYDRFRARLIFPIEDAAGRVVAF
                     GGRIIGEGEPKYLNSPESILFSKGRQLYGLNRAREEMRRQDCVILVEGYFDLIALYQA
                     GIRNGVATLGTALTRDHVDLLRRYTRNVAAVFDPDEAGRKALARSLEVFLAGGMQARA
                     VILPGGRDPDEFVRASGPEAFLEVIAHAPSAVDYYIDRMIGSQRSFEEKQAAIQAAVP
                     FMARIADPVSRELFIRRVAERVGIGEGLLKKEVSRGVPSSRAEVPVSAASRKPAPLDP
                     LEWSLLQLMLEHPETIPEISRSGILDCFLHEDLKRFGAYLVKQGCEGHGQTPSIQDLL
                     AAWPEDPIRERVQRLSVTASPYDPGMVDKIRVDTIHRIRRRWFRGKHRLLAEALRDAQ
                     DRGDQAGSEELLRRKESLLREEQGVDINRSKG"
     gene            80606..82360
                     /gene="rpoD"
                     /locus_tag="PLW16_01295"
     CDS             80606..82360
                     /gene="rpoD"
                     /locus_tag="PLW16_01295"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPB69570.1"
                     /translation="MAKEIQSDEFKKLMTLGEEKGFLTYDDVNDLLPSDVISSDQIDD
                     IIILFGEKNIDIIDTDKGDKIVVKKSPGELVEHKEEEDVLGVISAGKTGDPVKMYLRE
                     MGLVSLLSREGEVEIAKKIEEGNKEIMDAIFGMAVSVKDVIDIGAKIRAEELRIKNVV
                     ENLEDEDGFVEEDLHRERVLKLIEKVAMLDRKNDVMRQKLRAKGLAEKEKALFHEELA
                     KNTKGIVKYCRKVRFSKRQIDRMVFRLKSYLLEIEEAEREIQKAKDLTGLNIKEMERL
                     WVRLRKSPQEAKLAARERRVSLERLKATEPRVLEAQRQIRRILLETGLPLAALKRAVL
                     SIEKGEWKAEIAKRKLVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
                     QRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGREPT
                     PEEIANKMEYPLEKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKKVMSPSEATIN
                     MDLAEQTRKILSTLTPREEKVLRMRFGISERFEQAFEEDRDLFEVKQIEIKPMDAGQG
                     RKLRNPSQRRLAAPLRAK"
     gene            82430..82505
                     /locus_tag="PLW16_01300"
     tRNA            82430..82505
                     /locus_tag="PLW16_01300"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82463..82465,aa:Ile,seq:cat)
     gene            82680..83405
                     /locus_tag="PLW16_01305"
     CDS             82680..83405
                     /locus_tag="PLW16_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPB69571.1"
                     /translation="MKDTISLLVALQTLDSEAVQVAARKQSLPEKIRLLEETRQKQQG
                     ALDEAEEKLRGLTQRHRDQEEQLKRGVEGLKKAKQRLLDVKTNKEYQAILKEMEVIET
                     LQGKVEDEIIALLEQVDHMKAAVKQHEIDLSAFLAVFAQEKQELEGQVQALDDNLARL
                     KKKRQTLLEALPGDLVRRYETIREKRNGIAVISVWKEVCNGCHMNIPPQMYIELQRSQ
                     ELRTCPNCHRIIYWRNEASVGES"
     gene            83447..83935
                     /gene="rnhA"
                     /locus_tag="PLW16_01310"
     CDS             83447..83935
                     /gene="rnhA"
                     /locus_tag="PLW16_01310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPB69572.1"
                     /translation="MVHTDGACLGNPGPGGYGVILSYRGRSKELSGGYRLTTNNRMEL
                     MAVIVGLEAVKPGYDVTVHSDSRYVVDAVTKGWAVRWRAQGWMRNRKDRAENADLWER
                     LLDLCRHRTVRFLWEPGHAGHPENERCDALAVAAAKGSDQAVDAGYERASPRGDSGRT
                     GV"
     gene            83968..>84157
                     /gene="rnpB"
                     /locus_tag="PLW16_01315"
     ncRNA           83968..>84157
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW16_01315"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT        15343 a        25602 c        27923 g        15289 t
ORIGIN      
        1 ccggtctgcg tgatcgacga cggctcgacg gacgcgacgg ccgcgcgcct ggaggagatc
       61 gtgggggtcg ccgtgctccg ccacgagacg aaccggggca agggggcggc gatcctcacg
      121 gggttcgacg cccttgccgg ccgggcggac tgggctgtga ccctggatgc ggatggccag
      181 cacgacccga acgacatacc tggaatgatc cgggcgatcc cggcggggag ccggccgatc
      241 gtggtcggcc gacgggaggg gatgatcggg gccgatgtcc cctggacaag ccgcttcggt
      301 cggaaatttt cgaatttctg ggtccgggcg gccggggggc cggtcctttc cgacacccag
      361 agcggtttgc gcatctatcc gctgccggag gcggcccggc tgggcgtccg cgcccgccgc
      421 ttccagttcg aggtggagat ccttgttcgg gcgcactggc gcgggattcc cgtgatcgag
      481 gcgccggtgg gcgtctccta cacgcccggc atgaagcggg tctcgcattt ccggccgttc
      541 gtcgatttct gccggaatgc ggagacgttc ggccgtctga tcgcgttgcg tatcctgcgc
      601 tttcccggga ggggagggcc gcgaccgaca tccgatgagg ggtgagccgt tgttcttccg
      661 ccgctaccgt ttccgctggt tcctgatcct cgccgtattc ctgattctgg gcggtctttt
      721 tatctatacc gcccgcggga tccggatcga gacggacatc ctcgcctcgc tcccccaccg
      781 ggatccggtc ctggcggacg cccaccgcgt catccgccac ctgccggtcc aggaccggct
      841 ggtctgcgat ctcgagatcc gcggcgggga ctcggaaacg ctggtcgagg cggcggagcg
      901 gttcgagacg gggctgacgc ggagcgggct tttccgaaag gtcggcctgg gcgagatgca
      961 ggccctcggc ccggagctcg tggcccacgt cgtcgggcac ctgcccctcc tgttcgacga
     1021 acggcagctc cgggaggagg tcgtcccgcg cctcgccccg gaacgcatcg aacggcagct
     1081 cgcggagaat ctcgatctcc tccaggggct ggagggcatc ggtcaggcgg agcttgttgc
     1141 ccgggacccc ctggggctga gaaccctcgt gatggcgagg atggcccaac tcctgccggc
     1201 gcgggaggcc cgcttccacc gggggcagct cctctcgaac gacggcgccc acctcctgat
     1261 gacggccgag ctggccggcg ccgcgacgga cacccgcttt tcgcgggaca ttccgcccct
     1321 gctcgacgac ctcacgaaag aaatgaatgc agcctaccgt tcccggggcg tgtctttcgt
     1381 tgtcacgccc gtcggggcct accgcgccgc cctggacaac gaaacggccg cccgagggga
     1441 catgcggacg gcgatctggc tcacgacgat cgggatcgcg ctgctgctcg tcctgacctt
     1501 tccgcgcccc ctgatcgggc tcatggcgat cctgccctcg acggcagggg cactttccgc
     1561 gctctttgtc tgctcgctga tcttcccgcg cctgtccatc ctggccgtca gcttcggggg
     1621 cgccatcatg gcctttaccg tcgatttggg aatcgcctat ctcctcttcc tggaccgacc
     1681 tttcgagacg accgggaaac aggcggcgcg tgaggtgcaa tcggtggaaa ctctcgcggt
     1741 cctgacgacg atcggcgcct tcctgctgct caccgtcagc gaattcagcg ttctggccga
     1801 gatcggcgtc tttgccgcgc tgggggtggc ctgcgcctac gccttcgtcc atgtcgtctt
     1861 tcccctgatc atccccgtca tgccgccggc gaaacgggtc cggacaagcc cgcttgcgac
     1921 gctcctggac cggatcgttt cgtcggaggg gcgcgggcgc gggagactcg ccgccgcggc
     1981 gctcctgttc ggggtgatgc tctgtttctc acgacccgtc tttcaagtgg acctgaacgc
     2041 catgaattcc ctgagcgcgg ccagcatcgc cgccgagaaa cgggttcagg cggtttgggg
     2101 aaatttgacg agccgcgttt atctgctcac ggagggagcg ggtcccgagg cgttgcagga
     2161 taagagcgac gccctggccc cctggcttga ggaagacgaa agacgcggcg tgatccgggc
     2221 gcccttcgtg ctttcggacc tctttccggg cgaggcgcgg gcgcgccgtc aggccgaggc
     2281 ctggcgggcc ttctggacgc cggagcggac ggcggacgtg gcgcggtcgc tcaagcggtc
     2341 cggcgatggg atgggcttca gtccggctgc gttcacgccg ttcctaaatt cgctgaccgc
     2401 cgcaccgccc gcgacgcctg acgttccgga gcgccttgcc ggtctgctgg gcctctcgcc
     2461 gggaccggag ggtccggtgc aggtcacgat ggtgacgccc ggccccgcgt acgacgcccg
     2521 ggcgttcttc gaccggtatg cggccacggg gctcgtccgg atcttcgacg ccgggctttt
     2581 cagcaagcgc ctgggggatg tgctggtgac gcttttcacg gaggtcgccc tgatcgtggc
     2641 gatgggcatc acgctcgtcg ttttcttctt cttcctcgac tggcggcgga ccctcatcgt
     2701 gctggcgccg gtggtcttcg ccctcgtctg caccctgggg accctgaaac tcctcggccg
     2761 acccatcgac atccccgggg tcatgctctg ggtcgtcatc atgggcatgg ggatcgacta
     2821 cggcatctat tatgtctgcg cctaccagcg ctgcctcgac gagcacgact cctccatgcg
     2881 cctgatccgc ctgtccatac ttctggcggc ggcgacgacg ctgatcggct tcggcgttct
     2941 ggccatcgcc gagcacgccg tcctcaaaag catcgggctg acctccttct tcgggatcgg
     3001 ctattccctg ctcggggcgt ttgtcctcgt cccacccctg atccggcgcg tcctggcgcc
     3061 ggtggcgctg cccccggaat ccttccccgc cggttcgccc cggcatgagt cgcgggtgcg
     3121 cctgcgctac cggcatctcg cggcccatcc ccgtctcttc gcccgcctca agatgcacct
     3181 cgatccgatg tttccccatc tggccagctt tgtgagcgcg ccccgccgca tcctggacat
     3241 cggctgcggc atcgccgttc cctccgtctg gctcacggag ctgtatcccc aggcgcgcgt
     3301 attcggcatc gatccggacg aggagcggat ccgcgtcgcc cggcaggcga tcggcccccg
     3361 cggcgagatg cggaccggac gcgcccccga actcgatgac ctgccggagg aggccgacac
     3421 ggccctcgtg ctcgacctta tccacatgct cgacgaagcg gcctggcggc gaaccctcgc
     3481 cgttttgcat gcaaaactga tcaccggcgg ccgactcatt ctgcgggcca ccgttcccgc
     3541 ggaccggccc ttcccctgga agcggcgcct ggaggagttc cgcctgaagt cccatcgcct
     3601 ggccccccat taccggagcc ggacggcgat catggcaggg atcgcggcgg ccggctttga
     3661 tctcgaaacg gtggaggcgg acgggccggg cagtgaagag gtctggttcg tcgccgtccg
     3721 gtccccgtcg gacccggtcg aagaggcatc gcggacatcg gagggggtgc ggccgtgaag
     3781 gaccgcttct accgcttcct gaccttcctg acgcggcacc tgggcgcgtg ggtctttctt
     3841 ctggtcgcct ggttcatcgc cacgggctac ttcctcttct tcccgcgccg cgtcgccgtc
     3901 agcgtgcgct tctaccgccg cctgcttccc ggccgatcgc gtttccaccc cctgtggtgc
     3961 gcgtggcggc agtatcacca cttcgtgcgc gtcttcctgg accggttcct gctgacggag
     4021 ggggcggatc tcccgacgac gtacgaaggc tgggaccatc tggtggcggc ggcggagagc
     4081 ggccggggcg gcatcgtgct catgtcccac gtgggaaact gggagatcgc cgcccacctc
     4141 ctcaagcact atggacggag ccacccgagc atgaaactgc tcctgtatct gggggagaaa
     4201 caccgggagc agatcgagcg gcggcagaag aagagcgtcg ccaggaacgg catccgggtc
     4261 attgccgtgc cggaggaggg gggctcgccg atggacatcg tcgaggggct gaatttcctg
     4321 cgggaagggg ggctggtgtc catgacgggc gaccgcctct gggggcggga cccgcggacc
     4381 gtcgacgtcc ggtttgcggg cgctcgcgcc cggattccgg agacgccatt cgttctcgcc
     4441 ctgatgtcgg gggcaccgct cctgatcttt tttgcctacc gcacgcgcgg gggggtgtac
     4501 caactgatca cgaagccgcc cctgaccgtc cgggcggcct cgcggagcga gcgcacggac
     4561 gccattaccg ccgcggcgca gacctacgcc gacaccctcg aggagacggt gcgtgcccat
     4621 cccttcgagt ggtaccactt cgagccgttt cttctcgaag accccgcggc cgggccgggt
     4681 taatgaacgc cgcggcgtcc ggcgccgtgt ttatcccttt ttggaaaacc ccgcgagcag
     4741 tcgatcgagc atgatcccga agtcgggcgc cgtcgtcgct tccaggagcg ccgcatgctc
     4801 gaccgacagg tgaacaagca gatccattcc cgccgagccg tagaccatgc gcttgatggc
     4861 cgacgccgtc ttggccggga tggcggcgat gcgctcggcg atggcctggg tctcttcgat
     4921 aagccggtct tcggggaaaa acttcgtgat gattcccatc ttttcggcct cgcgctcgtg
     4981 gatgtcctcg cccaggagcg ccacttccag agccctggcg gttccgatga gcctgggcag
     5041 ggtccagttg atgcctccgt cgccggtgag cccgatggtg gaataggcgt agcgcaggcg
     5101 cacccgctcg gtggccaccc ggacgtccga ggccagggcg agggcgagac cgtatccagc
     5161 cgcgaggcgg ttgacggcgg ccaggaccgg cttggggctc tgccggacct cgacgatcgc
     5221 ccgatgcgcg atggtggcga gttccccgat gatgcgggtg ggatccgggg ccgcggtgac
     5281 ctccgtcacg tctgcgccga cggagaagga tttgcccgca cccgcgaaga cgatgacccg
     5341 ggtggccgga tcgtcgacgg cgcggaccag ggccgagaag gcccccttcg ccaggtcgag
     5401 gttgaaggca ttgtaaacct ccggccggtt gaagatgatc caggagaccg gaccgcgggc
     5461 ttccgtcagg acgatttctt gagacatgcg ctgttcctcc cccccttgat gattaccgtt
     5521 ccgggcgtgc gatgaacggt cggaaccgcc ccgggtccgc acccctctcg aggcggacgc
     5581 cgcgcgcttc gaattatacg ggaccccaag cgttatgcag acgggtcccg ggcgtcgtca
     5641 tccggcgccg gctcgatcga aaggcccctt cacgccgtcg ttcccgcccg aagctccgct
     5701 ttttcagcta ggggtcacgt cggacaagag ctgttcgatt ttttcccggt cgatcttacc
     5761 gttcggcagc gtagggatct ccgcggcgat ccggaggcgg cgcggacggg catgaggctc
     5821 gcccatcgtc tggacggccg ccttgagttc cgccggatcg aggtcgcccg ccacgagggc
     5881 cgcgagttcc acctggcgcc cccgcttcag gggaagggcc agaacgtgcg cgtccttcac
     5941 gccgggaagg cgcctcagcc ggttccggac ctcctccaga tccacccgct ttccgccgac
     6001 cttgacgatg ccgtctgctc ggcccaagag ccggaagccg tcttgcgccg gggcgacgcg
     6061 gtctgaggtc agaaaatatc cctccgcgtc ccgcggcaga tccggggaga tgaacgccga
     6121 gcgtaccgcg aggcgatcgg agacgatctt ccaatccacg acgcggaagg ggatgcagac
     6181 gcccgcggcg gacgggtcgg tgcgcagggc gatccctccg gtttccgtcg agccgaagat
     6241 ctccgtgacg ggcaggcccg ttctttcccg gaagaaggcg ccgtcctcgg cgtccacggc
     6301 gccagctgac gagagggcga gacggagcga aaagcgcttc agatcagccg ttttgagtgc
     6361 gcgataatgg atggggacgc agaccaggac cgtgacgtcg gccgaggcga gggcggcgag
     6421 gatttccctc gggaacaggc aggttccggg aagaatccgg gcctccgcgg cgaacggcag
     6481 aagcacggag cacaggagcc cgtagatgtg ctgcggcggg acggtggcga gcagccggtc
     6541 gctctttcgg atgcccaggg ttcgggccag gaagaacgcc tctccgaaca ggttccgggg
     6601 ggtcttggac caggaacggg gcttgccggt cgatccgccg gtaaagagag tcaaaaaaac
     6661 ctcgtcgggg gagcggacgg gggcgagatc gccgtgctcc ggccggcggg tctcgcaggg
     6721 gacggcctcc acgccggcgg ggatatcggg tgcggtgtcc gtcaggatgc gatggaaggg
     6781 caggacctcg cggacctctt ccaggacccg gcggtcatag gcgtggggca ggatgaaacg
     6841 gggaccgccg gccagggagg ccaggagcgc cgcgaggaga agcgaacgat cctggaggca
     6901 caggcagacc ggcgcatcct ccggctcgcg gagtcgggtg aaggcatccc ggatgcccct
     6961 tgcctgcagg cagatgtcgc ccagggtgct gacgccgtcg acatacggac ggtcgggatc
     7021 cgagaggcgg gaaaagatgg attcgatcat cggtcatcct cgagcggggc gttccccgaa
     7081 gatgaggcga ccgatccgga cgatcgtggc cccttcctcg atggcaaccc ggtagtcctg
     7141 ggacatgccc atggacagtt cctccatgac acagcattcg aggcactcgg cccggatctg
     7201 atcccgaaga cgcctcaggg cggcgaagca gggccgggat gcctccgggt cgtcggacca
     7261 ggggggcagg gtcatcaggc cccgcaccga cacgtttttg agggccgaga cggcgtgaac
     7321 cagcggcagg gcctgggaaa gcgggacgcc gctcttggac gcctcgctgg tgacattgac
     7381 ctcgatcagg atatgcgtga cgcggcccgc ggcggccgca cgccggtcga tctcctccgc
     7441 caggccgatc cggtccaccg aatggatcag ggtgaagagc ttcacggcct gtttggcctt
     7501 gttcgtctgc agatgaccga tgccgtgcca gggcacgtcc gtgcccaggc gttcgatctt
     7561 ccgccgggcc tcctggacgt agttttcccc caggacgtcg acaccggcgg cgagggcttc
     7621 ccggattcgc gcctcgtcca ccgtcttggt gacggccatg agacggacgg cggagggggg
     7681 gcggccgctc ttcgcggcgg cgtcggccat gtccttacgg atgcggcgga tgcgtgcggc
     7741 gatcgtgtcg ttcatggggt ttcctccggg gcaaagacga tgacgccggc ctgcttcagg
     7801 gtatccacca cttcggccag gggcaggccc acgacattgg tgtaggagcc ccggatctcc
     7861 cggatgaaaa aggcgccccg gccctggacg gcgtaggccc cggccttgtc gaagggttcc
     7921 cccgaccgca cgtaccaggc catctcgtcg gccggaatct ccttgaagca gacggaggat
     7981 tcgaccgccc cggtgaggga gagcccccgg tcatggcggc agagggagaa tcccgtgaag
     8041 acccggtgct cccggccgct cagtttgccc agcatgtcca tcgcctccgg cggcgatccc
     8101 ggtttaccca ggatttcgtc cccgatgacc acgatcgtat cggcgcccat cacccaagca
     8161 tgcggatgcc ggcgggcgac cgtctccgcc ttggtccggg ccaagcgccg gcagtggtcc
     8221 gccggcgcct cccccggcag aaaggtctcg tcgacgccgc tcgtccgggt ctcgaaatcc
     8281 agtcccaggc gacggaggag ttcgacgcga cgaggggagg ccgaggccag gatgagcggc
     8341 cccgtcatcg cgatgcgcgc ggcggagggc tcagcccccg cccgagaatt cccgtcctga
     8401 agatgcatgg tgccccgctt ttcaagaaat cccttgcctg atttttccca ccgtcctata
     8461 gaggaaccgt gccgccctgt caagcggcgt tcgacggcgc ggggtggcac gaaccctgcg
     8521 ggcgggaggc gatcgacatg aggttttggc aaggcaccgg gtcagggatg atcggcgtgg
     8581 ggctgatcct gctgctggcg gcgccggtga tggccgccac gcggcacgtg cagatggata
     8641 ttcccggatg catgacctga agcggctatg cgaggataag ttctatcctc aagcaaatcg
     8701 aaggcgtcac gtgctaccgg gcgacgggca agaatgagtt gacggtcgtt taccgggacg
     8761 aagaggtcag ccttgaaacc atcctggagg ccctgcgcag agggggcgtc gaatggcggg
     8821 gcaaacccac ctccctgccg ggcttgcccc ccggggcgtc tccgggggaa cccgcgcgca
     8881 gctcctagcc ttcgcccaat tgcccgccct tatagatagg cccggcccgg accctccgcg
     8941 aggaggttca cgaccttctt cacgtcctgg gatgcccctc gtctgcagat caagagagcg
     9001 tcctccgtct ccacgacgat caggtcctcc acatcgacca gggccacgag cttcttcgga
     9061 ctgaacacga aggagttgcg agcctccacc ccgacgaagt gcgccccgga ccggacggcg
     9121 ttgcccgccg cgtcccgggg cgagacctcc cagagggcgt cccagctccc gacgtcggac
     9181 catccgaagt cccccttgat caggaggacg tcgcgggcct tttccatgat accgtaatca
     9241 atggaaatgg gggtcatcgc ggcgtaggcg tcgcggagga cgtccgattc acggggggtg
     9301 ccccaggcga cgtcgatctc ccgaagtgcc cggtaaatat cgggcaggtg ccgcgcgatg
     9361 tggtccagga tgaccggcgc ctgccagacg aacatcccgc tgttccagga aaaattaccc
     9421 tgccgaagga aggactccgc ctgggggcga tcgggcttct cgcggatgga tcggacccga
     9481 tagaccgtct ctccggtgac cgttccagcc ggctcgccgt tttccaggta gccgtatccg
     9541 gtttcggggc cggtcggcgc gatgccgatg gtcacgagcg ccgcctcacg gtcggcgacc
     9601 cggacggccg cggccaagac atgaaggaag cgcgcttcgt cccggatgag atggtcggcg
     9661 ggcaggacgg ccatcacggc gcccggcgag cgccggttga cggccagggc cgcgaggccg
     9721 atgcagggag cggtgttgcg gcccatcggt tcgatcagga tgttgtccgg cgggacctcc
     9781 gggagctggc gcatcagctc ctccgcgtga ctcgcccccg tgacgaccag cgtccggtcc
     9841 ggcgggatca ggggggagag ccgggcgagc gtctcctgga tgatcgtccg cgtacccacg
     9901 atgtccagca ggtgcttggg catccgctcg cgactgcgcg gccagaaccg tgttcccttg
     9961 ccccccgcca tgatgacggc gtagatcgac ttgccgccgt ccggattgcc gatttctgca
    10021 tgcatgcggt tatccttcct tccgcttctt gatctcgagc gccgccttga gccctttttc
    10081 acggatcacc ttgaaggcca ggcccctggt tgtctggcgg tagaagcgcc gggcgtattt
    10141 tttgagcatc tttcggtcga tctcgaatcc caggccgggg cgcgtgaagg gccacaacat
    10201 ggcgttcttg tcgggcagga tgggcgcgat gaggccgtcg cggaattccg gcacccagga
    10261 cggttcctcc agtggatatt cgagcggatg ggtgtttccc cgatcggcca ggaccaggtt
    10321 ccagttgatg tagaagccga tcccgttggt ccaggtatgc ggggagtatt cccggtcgtg
    10381 ccggtggcag gcatcgatga cctgcctgac ctgcgcgatc cctccggcaa aggtggcgtc
    10441 tggctgatac acgtggaagc agtccttctc gagcatgacc ttgatctcgt cccacccgtg
    10501 gttcagctcg gcgcccgaaa tcttcacggg agaggccttc ttcagcgcgg ccagcccgtc
    10561 gtaatcgtgc cagtccagcg gctcctccag ccattcgatc ccgttgtcgg cgcaggcccg
    10621 cgcgtaatcg cccgcccgat tgagatccca ggcggggatg cggtccacga tggaaaccag
    10681 ccatccctgg ttggcgtcca cgcccagggt cggaccgtcg cccaccccct tgcggacctc
    10741 ttcgatcagc tggacgtcct ccgcgaaggt tttggccttc gcccgcagct tgaaggtttt
    10801 gaagccgcgc gccatcaggg acaggatttc ctccacgcgc cgggccggtt cgtgcagctc
    10861 gccgaacgag cagtaggcct cgatggctcg ggcctctccc ccgaggagct cgtagaccgg
    10921 tttcccctgg gccttgccca tgatgtccca gcaggcgggc tcgatccagg ggttaagcca
    10981 gcccaggtag ccggcctgct tgagcaggct ctggatgcgg tcgatatcgg tcggatcggc
    11041 gccgatgatg tacccgccca gcaggtcgcc cagaccctgc cgttcctccc ccatggccgt
    11101 cccggcggac catccttcga tcccgtcgtc cgtgacgagc cgaatcaggg tgaagcggtt
    11161 gtgcgtctgg ggatagccgg ggatccaggt cggccaaaag gtgtgtttga gggggatatg
    11221 gacgtgataa agctcgatct cggtgatttt catcgtgccg cctccttcca tgagtcattg
    11281 cctgggaccg ccggcagtct tgatcccgct tcatgcgccg tcttttcgat cccggtgaat
    11341 ggaggcgaag tatacgaggg gcggtccggg cttgtcaagc gtggaatccg gagaattctt
    11401 gcattttcgg gacggaatgg atagacttcc cccgcgcctc gcccggaggt ccgcggggaa
    11461 cggaagtgtg cggggccaca ggcgcaggag gatgtcggcc atgacgaaaa agccggtcta
    11521 tccggtcgtg acgctcaagg cgggacggga ggactccctc agacggggcc atccgtggct
    11581 cttctccggc gccctggcgc ggattccgga gggactggac cccggagagg tggtcacggt
    11641 ggcctcggcg tcgggcgatg cgctggccct ggggttttac aatccccaga cggacatcgc
    11701 cgttcgcatc ctgacacgag actgccgggc ggggatcgat gccgcctttt ggcgcgagcg
    11761 gctccaggtg gcgcttgcgt tgcggcgacg agtggttccg gccgatacga ccggctaccg
    11821 tctgatcaat gccgagggcg atggggtgcc cgggctcgtc gtcgaccgat acgaccgctg
    11881 gttggtcctg tctatcgcca cggcgggaat ggacggcttt cgggaggact gggtgcggat
    11941 tctcgaagag gcgctcgctc ccgaagggat ttatgagcgg agcgaggggg cggcgcgacg
    12001 gcgggaaggc ctgtccgacc ggagcggcgt tctcgcgggg gcggcgatgc cggaggtggt
    12061 gccgatgtcg gaaaacggct ttccgtttgc agtggacgtg gaagccggac agaaaacggg
    12121 gtttttcctg gatcagagac ccaaccggga gctgtccggg aggctggccg cgggcgcggg
    12181 atgcatgctg aacgcctttg cctattccgg cggcttttcg gtttacggaa tccggggcgg
    12241 ggcggggcgg gtcgtctccg tcgacatttc cgagcgggca acggcgcttg cgaaacgaaa
    12301 tcttgcgctg aacgggctgt ccgaggagac gcatcccgtc ttgacggccg atgtcttcaa
    12361 atatctccgg gaaaccgacg aaaccttcga cctggtcgtg ctcgatccgc ccgcgtttgc
    12421 gcgcagccgc aaggatctgg cgcgggcggc gcggggttac aaggacatca acctccaggc
    12481 gatgcgtcgc ctgaccgcgg ggggactgct cctgaccttt tcctgctcga atcccctcga
    12541 tcaggatctg ttcgagaaga tcgttctcgg cgcggcgcgg gatgcgatcc gaacggcccg
    12601 ggtgctgcag cgcctgggtc ccggcggcga ccatcccacc gatctggggc atcccgaagg
    12661 ccgctatctg aagggcctcc tgctttgcct gtagggcggg tatccttcaa aagcgcccgt
    12721 ggcgtcccgt tccgttccgg aaccgcgccg cgccctcctg ggtctctccg ctgaaaatca
    12781 cctccaggcc gcggcggaat tcgttttcca tcgccgcgtc gaaggcctga tcgaaccctt
    12841 cgtaagcggc gaggcggtcg ctgcgaagac agcgctgcgg gaacgcggcg atttccgcgg
    12901 ccagggcctc cgccgccgtc cgggccgcgc ccggttccac cactcggttg acgagcccca
    12961 tggcgagggc ctcctccgcg cccaccggcc gtccggtcag gatcagatcg agcgcacggc
    13021 tcatgccgat caggcggggg agccgctgcg tgcccccgtc gatcaggggg acgccgaagc
    13081 ggcggcagaa cacgccgagg acggcgttcc gctccatgac ccgaaggtcg caccagcagg
    13141 ccagttccaa gccgccggcc acggcgtgac ccgtaatcgc ggcgatgacc ggcttggaca
    13201 gtttcatgcg tgtcggcccc atgggtcccg gggcggtcat gtccgtgttc aaccggttgg
    13261 cctgtccttc ggatagtctg gccaccgcct tcaggtcggc gccggcgcag aaggcgcctc
    13321 cggaaccgta caggacggct gcggcggcgc attcgtcccg gtcgaactcg ctaaaggccg
    13381 cgaccagccg atccgcggtg tcccgattga cggcgttgcg ggtctccggg cggttgatga
    13441 tgacggtcca gacggcaccg tttttctccg tgatcacttc catgccttca tctccataca
    13501 ggcccggacg ggcacgaagc gcggcctcag ggtttggcca gttgctgggc gatgcgctcg
    13561 tggagcatcc gggacgcgag attgttgccc gagacgccgg tccggatcgc gacggtcgtt
    13621 tcgttgcggg ccacgtattc gacctcgatg gagacctttt cgtcctggat gacggcctgg
    13681 atctgtccct gggcgatctt ccggaccggt ttgacctgcg tggccttcat gtcgctcagc
    13741 atctgttcgc aggcctccca cacccggtcg aaggaagcgg cgtagcggga gtgcagaaac
    13801 ccgtcggtgt agaggaaatc ccctgaagtg acgccgaccg tgcggtttcc gatggtcagc
    13861 gccgtgtcgc atcccaccat cagaatgctc agcagaagac acacccacag agtttttttg
    13921 atcatgatgc cctctccttc tcttgtcaga atggacggct gaaccgccgt gcttcccgta
    13981 tcgcccctgg ccgccggaga cggcggtcgg ggtgcggcca ctataggaaa atccgcttcg
    14041 ggaagtcaat cgaattcaga acggggcggg gacgtccgag aggaaggccc ggaccgtcgc
    14101 ccagaccagg acgacgagcg tcccgatggc ggtgacgccg ccgcaagggg cgagccaggc
    14161 cggttcgccg ttccgctcct tgtacccgag tgcgaaggcc atcagcaagc ccgcgaccag
    14221 gccgccgcca tgcgcccagt tgttgatgcc ggggacgagc agaccgaaga gcaccagtcc
    14281 gacgatccac ccggtggcct gccggtagac ggactcgccc caaaagcccc cccggctctt
    14341 tccgtagtat aggatggcgc cgataagtcc gcagaggccg gcggatgccc cgatcgtgaa
    14401 ggggatgccg gcgagatagg acaggagaaa tccggccgtg ccgctcgcca cgtacaggac
    14461 gaggaagcgg tgggtcccga agatctgctg cacgaagggc cccagctgcc agagggcggc
    14521 catgttgaag aacaggtgaa gaaggccgcc gtggagaaag gacgccgtga ccagcgtcca
    14581 ccagcgcccc tcgtaagcga tggggagaac gccggttgcc cccagccggt agagggccct
    14641 tccgtccggc gagaggaagg agaacggact gggggcgaga ccgatgccgg cgggcgtcag
    14701 gagcagggac aggaggtaga gagtgatgtt gacggcgatg agtcccggga cgagttgccg
    14761 ggaggcgagg ccgaagacgc ccagcgccgc ccgccagggc gcgcccggtc ttgtcaggcc
    14821 gcaatggggg caggccggtt catcggcgct gatcagcttg cggcagccgg ggcagagaag
    14881 cgatgttttt cttggggacg ccatgcgagt ctaaatgaac ctcatgatga acggaatggc
    14941 gatgaaggtg ccgaccgagg tggtgaggtt gacgatcgcg acgagcagca ggatgcgcgt
    15001 gatcttgttc cggaagaacc cgcggacgct catgatgtcg ttcttcagct ccaggaaatc
    15061 ccggacctgg ggtttgcgga cggacgcctc gacgaggccg gcgatccagc ccgcggcgag
    15121 cagggggtgc agcgtcgtga agggggcggc cagagaggcg gcggcgatcg tgaaggggtg
    15181 cgcgagcagc aggatcgctc cgacgctggc gcaggcggcg gtgatcgccg cccaccacgc
    15241 catcatcgtg aggctggcgc ggcttccgga ggcgaagaag cccgcgacga acagaccgag
    15301 gatggccagc gtgaaccccc atcccgccca ttttgcccag aggctcgggg gggggatccg
    15361 gttcagggcg ttgaggtcga tgatccgatc caggttcttc acgatgccgg ggacgtggcc
    15421 ggcgcccacc acggccacga tcttttgtcc gggcgcgtgg gagatcctgt gcgccagata
    15481 ctgatcgcgc tcgtcgatca gggtcgtctt gagctcgggc agcttcttgc cgaccgtctc
    15541 cagggccacc tcgaggacgt cctgctgctt gagcttctcg atgtccttct ccgtgatctc
    15601 ttcggagaag aagagggaaa aaaccatctc gctgatgaac ttcgtccggg cccagaatcc
    15661 catcgatcgc cagacccgga gcagggtgat gcggatttcc cgatcggcca ggaccagctc
    15721 ggcgcccgtt tcctccgcgc gggccatggc ccggagcatt tcctcgcccg gctgaatatg
    15781 gaatctctcc gcgagcttgt tttgaacgga tgccatgagc agctgggcga gcaggagggc
    15841 ggtccgtttt tcacggacca cgcggacgat gtccatctcc tgccacttgt ccttctgccg
    15901 gagggcctcg tagcgggccg cgcacaactc gatacagatg gtgtccggtt tctcctcgcg
    15961 gatgacgcgg tccacaagct cggcgctgtc gcgcgaaaca tgcgcggtgc cgacgagcac
    16021 gagttccttg ccggggtatt gaaaccggtg caggggttca gggttcaagg gggggggtat
    16081 ccttctcatg tcggggatgc gaaccgtcca tagcaggatt gatgccggtt gtaaagggaa
    16141 atggccgcgg cttcagcatt cgatgcgaag gtcgtcgcgg gcggcgaacg tctcggggaa
    16201 atcggcccgc gccgcggcct ccgcctccct tcgctccggc agactcccgt agatgcgggc
    16261 gtcgaagtgc gtcagcgcca ggcgccgcgc cccggcctcc cgggcaatcc gggcggccgt
    16321 ctccgggttc aggtgcggcc agctttccga agattgcccc gttttgtagg cgcactccgt
    16381 gatcaggaga tccgcccgac gggcgagggt cacggcgttt tcacagtatc ccgtgtcggg
    16441 acagtaggcg atgatccgcc cgtcgagttc caggcgaaac cccagggtca gccccgcatg
    16501 gacgaggggc agggcctgga cggggaaggg cagggcggcc gcttccccgg gcagctcgca
    16561 caggtggacc ggatagggca gcccgtccag cggcaccgta aagggggcgt tcacgaaggt
    16621 gcggaggacc gcgcggccgc cggccgggcc gcagagggtc agtccccccg ggagattgaa
    16681 tttcgtcagg gtgtggagtc cgccgatgtg gtccaggtga aaatgactga gaaaaatgta
    16741 caccggccgt gggtcttccg gtgacaggtg acggtccagt ttggcgaggc cgccgccggc
    16801 atcgaggacg acgaccgccc cgggtgcgga aatcagggta cagaccgtgc tccccgtttc
    16861 cgtgtcatac cagccgttgg tgcctaggaa acagacctcc atcccccgta tccctcctct
    16921 cccggggtca gcgtgacatc gccgggacgt cgccttgcct ctcccctccg tctcacggtc
    16981 atccggaccg gttcccggag agggttttga gcttttcctc cctgagaaag cggcgcgtgt
    17041 cccggatgac gttccggacc gcctccggca ggggggacac gggcgccccc gtggagaggg
    17101 cgcaggcgta gacctggcag gccttttcca gaagctcggc gttgagccac gcctcggcga
    17161 ggtccgcgcc cagcaggagg gcgccgtggt tctggatgat gtaggcgtgg gcgtggttgt
    17221 ccatccgcgc ggcgacgttg gccgccagct ccgggctgcc ggacagggcg taggagatga
    17281 tctccacctg ctcccccagc ttgaaggcga cctcgtcgaa cagcgccggg acgggccggt
    17341 tgatcaaggc gaagacgctg gcgaagggct ggtgggtgtg aacgatggcc cggatctccg
    17401 ggcgtttcct gtacacggcc gcgtgcagcc ccgcctccac cgacggacgc cgggcccccg
    17461 cccggacgtt caggtcgaag tcgagcaggc agaggtcctc ggcggtcatc tcgtcgtagg
    17521 aaaggctcga cggggtgatg aggaaggcat cgtctccgga ccgcacggag acgttgccgc
    17581 cggttccccg gagcgtgccg aaatacccgt gggatgagag ccatccgttg caggccagga
    17641 tttcctgttt cagggccgcc gtttcttccg gtgtcgtcat gggaaccctc cttccgtggt
    17701 tgtcaaagtt cataggccat ggagcggcag cgagaatagc ggaacggccc gaacttgtca
    17761 agcacgcgag acgtcggggg aggcgaagcg gagcggtcgg gaaacccaaa cgggattccc
    17821 tgttgacagg cgcggacggc gccggtaaaa gaggctgaag ggttgaggat acgggcgggg
    17881 gagggacagc catgaaatgg acggggaagc acgtggccct ggcattgggt ggtggaggtg
    17941 tccgcgggtt ttgccatatc ggcgtcctcc ggatcctgga aagggaggac gttccgattg
    18001 acctgatcgt ggggaccagc gccggcgcgc tgatcggggg ggcctacgcg agcggccagg
    18061 ccccccggga tatccaggcc aaggtcgatg cctatctgtc gagccccgag tttggcgcct
    18121 ccacgctgaa atccatcgga ctggcgatct ccccggagca gaagggccgc tggcgcaagg
    18181 cccgggccta cgtcctgaac cgattccacc tcgtccgcgc cttcttcagg ccggcggtgc
    18241 tccccaccga ggatttccgt tcgctcatcg attatttcct cccggaggtc gacattcgcg
    18301 aaacccgcat tcccttccgg gcggtcgcca ccgacctgat cacggggaaa caggtcgtct
    18361 tttccgaggg tccgctccgt caggccgtgc tggccagttg cgccgttccg ggtgcggtgg
    18421 agccggtccg gcacggggag tggctcctgg ccgacggtgg cgtcacgagc ctcgttcccg
    18481 tgaacgcggc gcgcgcggcg ggagcggacg tcgtgatcgc cgtgatggtg gatcgcgatc
    18541 tgatgcccga cggcaacatg gaaacggcgc agggcattat ccagcgtgcc ggtgaaatcg
    18601 cggccaacac actcgaagcc acggaactcg cgggggcgga cgtgatcatc cgccccgacg
    18661 tgggcgatct gcactggatg gacttcagcc gggcggggga gctcgtccgg aagggggaag
    18721 aggccgcccg agcggccctc aaacagatcg acgcttccct gcccctttac cggcggatct
    18781 cccgcctggg ccgccgcatc ttcaagggat gaatccttga cggacagtcg ccttttcggc
    18841 tctgcctttc tgaagatcgc ccaccggggc tactcgtcac ggtaccccga gaatacgctt
    18901 ccggcgttcg agggggccgt cgcggccggg gcggacatga tcgagctcga tgtccgtctc
    18961 tctcgggatg gcgaactcgt cgtgatccac gacgcgacaa tcgaccgcac ctctaacggc
    19021 cggggccgga tggctgatct gaccctggcc gaactccggc gcttcagcta ccccaacgcc
    19081 atgacggcgt tcggccgggt cccgatcccg accctggcgg aggtgatcga ccgcgtcggg
    19141 gcgcaggtga cgctgaacgt cgagatcaag gggcggcggt cggggtccgc cgggatggag
    19201 cgcttgattg ccgcgcttct gcggcggaaa ggggccgttg accgcgtcat cgtctcggcc
    19261 tttgaccggc gggcgctggt cgagatgaaa cgggcggacg gacaagtgag gacgggcctg
    19321 ctctacgacc ggggttggag gcgcttcggt gaagacgtgc ggcgactcgg cgtctacagc
    19381 gtccacccaa ggctcagcgc gatcgacgcg gaggcgctgc ggtgggtcca ttccgagggg
    19441 gtccgggtgt ttccctgggt tgcgaaggac cggggagatg tcgatcggtg ccgggcggcg
    19501 gacttcatcg acggcgtgat ggtcaacgac ctcgccctgt tcggagacgc atgcccctgc
    19561 ggtcacaacc cagataggac ttgataaaat cgaccgggag ttgtagaaaa ccgtcagaag
    19621 atcagcggga ggcacaggca tgtactggga aaaagagatc gaaacgatgg atcggaccgc
    19681 gcttgagcgg atgcaggtcg aacggttgac ccaaaccctc gaccgagcgt cccgctcgcc
    19741 ctactacgcg gagcggctca aggccgccgg ggtggatgcc cggagcgtcc ggaccgtcgc
    19801 cgatgtcgcg aaattccccc tgaccaccaa ggacgatctc cgggaacact ggccctacgg
    19861 atttcttgcc gtggagcggg acgagctgat tcgcatgcat tcgtcctccg ggacgacggg
    19921 ccgggccacg gtgatctttc acacgacggg cgacatcgcc gcctggacca acatcgtggc
    19981 ccgctcgatg tacatgaccg gcatgcgccg gtccgacgtc ttccagaaca tgatgaccta
    20041 cggcctgttc accggcgggc tggggttcca ctacggggcg gagaagatcg gcgccctcgt
    20101 cattccggcc ggcgccggca acagcaagcg ccagatccaa ctcatgcggg atttcgcgac
    20161 gacggtgatc cacatcatcc ccagttacgc cctgcacctg tccacggtgt tccaggaact
    20221 cggcgtcgat ccccgccggg acaccgaggt gaagatcgcc ttcatcggcg ccgaaccgca
    20281 ctcggagaag atgcgccaga agatcgagtc gttctacgga ttcaaggcct tcaattccta
    20341 cggactgtcg gagatgaacg gtccgggcgt ggccttcgaa tgtcccgaac agaacggaat
    20401 gcacctctgg gaggacaact acatcctcga aatcgtcgac ccggacacgc tgaagccgct
    20461 tccggacggg gaagaggggg aaatggttct gacgacgctc cagcgggagg ggatgcccat
    20521 tgtccgttac cggacgaagg acctgacccg ggtcatcccg ggggcctgcc cctgcggacg
    20581 aacgcaccga cggatcgagc ggatcaaggg ccggacggac gacatgctga tcctgaaggg
    20641 cgtcaacatc tttcccatcc agatcgagaa gaaactgatg gacattcccg gcgtcggaca
    20701 gaatttcctg atcgtcctcg accgggaggg gtacaacgac cacatgaccg tcaaggtgga
    20761 ggtcaaccgg gaattcttcc tgggggatct gaagcagttg gaggacctgc ggcgaaggat
    20821 cgtcgaagac ctcaaaagcg acatcctcat cacgccgaag gtcgatctgg tcgagccggg
    20881 cagcctgccg aagggcgagg ggaaggcggt ccgggtcgtg gacaaccgca aggactgata
    20941 ttcgacggcg ccgccacccc acgaaacccg aacgcttcag ggagggtcga accatgattg
    21001 cccagcagat ttccattttt gcggaaaaca aacccggccg gctggcggcg gtgacccgca
    21061 ttctggccgc ggaaaagatc aacatccggg cgacgaacat caccacggcg gataccttcg
    21121 gggtgatcaa cttcatcgtg gatgatccca agcgcgccgc gaaggccctc acccgggcgg
    21181 gaatgacggt gcagctccgc gacgtcctgg ccatcctgct cgaggacaag cccggcgggc
    21241 tcgaccggct gacccagctt ctggcccgtg aggcggtcaa catcaacaac gcctatggct
    21301 tcgtcctcga gggcagcaag aaggccgttt tcgtggtcga tgtcgaccag atgggaaaaa
    21361 cggaccagct cctccggaag cacggtatcc agacccttga tacggaggcc ctgtcgtcga
    21421 tcgaaccctt tcattacctc aattactgaa ggcggcgagg agtcggaaca tgacggaaaa
    21481 agacaaaggg cactttgcgg acaagcaccg gggagagcgg tcggtaagcc cggcactcgc
    21541 ggcggcgatt cgggataagg cgggcgaggg caagctccct tgccggcgcg ccgagacgat
    21601 ctgccgggaa atggacgtca cgatgggcga agtcggcaag gcggcggacc tgatggagct
    21661 ggagatcacg aagtgccagc tcggtctgtt cggctttgcg cccgaccgga aggcggtcaa
    21721 gcccgccgcc ggggtttctc cggagttcga gacggacatc cgggccgcct gcgtcgacgg
    21781 tcgcctcccc tgccgctccg cctgggacat cgcggcacgt cgccggattc ccaagatgga
    21841 cgtttccgcg gcctgcgaag ccctggagat caaggtcaag ccctgccaat tgggggcctt
    21901 ttaggccgag cgcgggggat cgggaagagc ctttgcggcg tgccggcggg agggcgcagc
    21961 cgttccgaag atatggccgg gtttctcagg gcggggttcc cggacggtgt ttcaggtagg
    22021 ttcggacgat ccgtcccatg cggtcggcgt tttcacgggc ggtggcggcg atctgcgccg
    22081 gtgccggcgg cgttgccata agcccgtgac cgtaattgtc gatggagcac agggaggcgt
    22141 aggggagttc gagttcgcac gcgatcacgg cctctccggc catggtcatg ccgaccatat
    22201 cggcgaaggc ggtcatcatc gcgatttccg cccgggtttc cagacgcggg cccgtcgtct
    22261 gccagtagat tcccgacgcc accacctcga ttccggcgac ccccgccgcg gcgatcagcc
    22321 gccgtcgcgc cggttcgtca aggccgggga cgacatgaac cggccggttc gagaagaccg
    22381 tgaacgttcc gtagagctcg atgaagtcgt gcggaacgac gatcgtcccc ggcgacaggt
    22441 gcatcttcag ggatccgacg gagttcaggc cgatgacctc cgtgcagcct agatccttga
    22501 gggccctcag attcgccgcg tggttgatcc ggtgggggag gatgtagtcg tccgggccct
    22561 ggccgtggcg ccggatgtac gccagtgttt ccgacacgtg gaccgtcgcc gtcccgaagg
    22621 ccgtttccgt aaccttcggt ctcagggtgc ggaaaggttc gaggtgctgc agggcggtgc
    22681 cggagatgaa gccgatacgg gacatggcgg gggacctccc gggacaggaa tcatgcaaca
    22741 ccgtttaaca gaaccgcggc cggttgtcaa aggccggggc gtcgaaatag ggtttgacat
    22801 ccggcaaaat tgtgtgagat aggttgccag cccccgggta cgccgtgatc gggccccgtc
    22861 gggccgggcg gcagggcgga aaggtttgag tgcgaaaaag ggaggtggaa gggacgccat
    22921 gggaaaagag gacttgatca tccatgtcac cgacgcggat ttcgatggcg aggtcatcaa
    22981 ggcggacaaa ccggccctcg tcgatttctg ggcgccgtgg tgcggaccgt gcaaggcgat
    23041 cgcgccgctc gtggaagcgc tggcggagga gaaccgcgat cggctcaagg ttgccaaggt
    23101 caacgtggac gacaacccga acacggcctc cgcctacggg atccggagca ttccgaccct
    23161 cctgttgttc aaggagggaa agctcgtcga taccctgatc ggcatggttc ccaaggaccg
    23221 cctcgaggcg tttgttggga aagtctttca gtcgtgatcg gtcgggtgag atcgaagctc
    23281 ccggcccgcg cggggcggga aagcgcagat gaacggtggc gcggcggcga ggcgtggaag
    23341 gccggtctgc tggcggcact gctcgtcctt ctcgcgggct gcagttggtt cagccatcag
    23401 gacatggccc gggcgacgcc cgaagggctc taccgtcagg gcttcgagga ttatcaggcc
    23461 ggccggtacg aaagggctat cgacaacttc cagcgcgtca aggagcagta ccccctgagc
    23521 cccctggccc tgcttgcgga gctgggcatc gccgacgccc atttcagcga caagaattac
    23581 gtggaggcgg aggcggccta catggaattt gccgggcttc atccgaccaa cgacaacctg
    23641 ccgtacgtcc tgtaccagat cggcatgtgc cactacaacc agaggtcgac gatcgaccgg
    23701 gatcagtcgg agacggtccg tgcacagagg gaattcgagc gcctgatcgc ccgcttcccg
    23761 gacagccggt tcgccttcat ggcggaaaag aagctgatcg attgccgacg gaacctcgcc
    23821 gaacaggagt tctacgtcgg ccggttttat ttccggtcga agaaatacgc ggccgccctg
    23881 aaacggttcg aagggattgc gcgggattac gcgaacctcg gactggacta caaggtggat
    23941 tatttcctcc gggaaacccg ccgccgcctc gccgaggagg aggcccggaa gaaggccatg
    24001 ggcctgaccg acaccgcccc cgctgccgcc ccaaccccct gaggtttttt gaagaaccgc
    24061 attcagcgtc cctgccggat gccgcggagg gcctcggccg tcatggcctc cggtgtcgtt
    24121 ccggtgtcga cggcgatatg gacgccggat gcccagccgg acatggggtc gaaatccgcc
    24181 cgctgggcct cgaagatgtc ccagcggccg tccgaaggtt cctctgcgtt tgccgcacgg
    24241 gtgtcgagtc tacggcggat caacgcctcc ggacagaggc aatccaggat gtaaaacgcc
    24301 gccccgtgac gccggcccag cgcctcggcc cggcgccgtt cgtcgtcccg tttgtaggac
    24361 gcgtcgatga taacggagtt gcccgccgcc aggaggtcgt cggcgcgctg cagggccgta
    24421 tcgtacgttt tgcgggagat ctcctccgaa tagattccct cgccgaagtt ttcgtagcgg
    24481 cggtccgtcg gtgcgagtgc aaggatctcc ttgcgcagga cgtccatccg gatgattgtc
    24541 gcgcccagga gcggggcgag acgacgagcc agcaagctct tccccgtgcc catgaggccg
    24601 gcgaccagga tcagggccgg ccgctccagg tgcgcggcgt aggtgtaggc gaggtcgaaa
    24661 tagcgtccgg ccgtccgggc cgcttcgtcc cggtccggag ccgcgatcgc ggtgtcgtcg
    24721 aggcggaagc ccacgacctt tccccgcacg taggcgtaat agcacttgta gaagttgagc
    24781 agggtccgga tctccgtgtc gcccgtgcag cggatgtagg cctcgacgaa gtggactgcc
    24841 tcgacggcgt agccgttgaa atccagatcc atggccagaa aggcgacctc cgccgccaca
    24901 tcctcgtagc ggaagcgttc gttgaactcg atgcagtcga agatcaggat atcgtcggcg
    24961 accaggatgt gttcgaggtg caggtcgccg tggcagtctc ggatccggtg gtcggcaacc
    25021 ctctggtcga aaagcgcccg ctcccgttcc aggaagccca gggcgtaggc gcggatgaac
    25081 cggtactgct cggcggggat cgtccggccg atgaacggtt ccgtctgttc gaaattctcc
    25141 tcgtgattgc gccggatggt ttcgatccca ccgatccggt cgatctcgcc acccgtctcc
    25201 gcctcgcggt ggaaggccgc caacttttgc gcaacggctt ccatcgtctc gggtcgcatg
    25261 gaaccctcgg ccaggaggcg cttcaacatt cgctcctggg gaaggcgccg catcttgacg
    25321 gcgtattcga cgacgggtcc tgctccaccc aaccgtggct ctccgttagg gtcggccgtg
    25381 acggccacaa cctccaggta cgcctgcggg gccaggcggc ggttcaggcg gagttcctcg
    25441 ttgcagtaat gccggcgctt cgccagcgtc gtaaagtcga ggaaaccgaa atccacggct
    25501 ttcttgacct tgtaaaccgt atctccggcg aggaaaatga gtgaaatgtg ggtctggacc
    25561 aactcgacgg cgtccgggcg atcgggatag aactccggcc ggctcatggc ggcgacaagc
    25621 tgcgggtgcg tcatggggaa acctcctgtt ggacggtccg tccgctgagc tgtccgcagg
    25681 cggccaggat gtcgctgccc ttgctcgcgc ggaggatggc cgtgtagtgg ttgcggagca
    25741 gaacgccctg gaaggcactg atcctttccg gcgtgggggt gcggaagggg gcgccgggaa
    25801 attcgttgaa ggcgatcagg ttgagcttgc agcgcaggcc cttgagcagg cggcacaggg
    25861 cttcggcgtc cgcgggcgcg tcgttgacgc cgtccaagag gatgtactcg aaggtcagca
    25921 tgcgacggcc gggcatgggg tagtcgcggc aggcctggag cagcgcgctc agggggtatt
    25981 ttcggttgac gggcatcagc cggcttcgcc gttcgtcgtc gggggcgttc agggaaatgg
    26041 cgaggttcac gcagacgtcc cggccgagtt gttcgatccc gggggccagg ccgcacgtgg
    26101 agaccgtgac cttccggctg gaaaagccgt gtccgtaatc ggaggtgagg atgcggatgg
    26161 ccttgagcgt gttgtcgtag ttggccaggg gttcccccat gcccatcatg accaggttct
    26221 tgatgtccgg gccttcgggg gtccggagct taaggaccgt gagctgcccc gcgatttccg
    26281 cgggggtgag ctgcctccgg aacccgagtt ttcccgtcag gcagaaacgg cagcccatcc
    26341 ggcatccggc ctgcgtggag acgcagagag tccagtggtt tcttcccggg atgaggacgc
    26401 tctcgatgcg ctcgccgtcc gagaggcgga acagggcctt cttcgtcccg tcccgtgaaa
    26461 cctgcgtctc gaccagttcg ggcaggtcca gccgagcccg ggcgccgatt tccgcacgga
    26521 agtccttggc cagcgtggtc atcgccgtga agtccgtgac tccgaaggca tgcatccacc
    26581 tcatgatctg gcgggcccgg tagccctctt ttcccatgcc ggcaaggaag gcctccaact
    26641 cctccagtgt catgttccgc aggttgggcc tatccatgga gcttccgctg caacctctcc
    26701 gcgacagcca gaatgaaggc ctccgtggag accttccggt tatcgggaga cgggtccgcg
    26761 acggcgagga gatcccccgt cataacaccc gcttcgaccg tttcgagggc ggccgcctcg
    26821 agcgcgtcgg cgaaggcgac cacctgtggc gtgccgtcca gttcaccgcg cttgcggagc
    26881 cccccggtcc aggcgaagat cagtgccatg gaattggtcg aggtctcctc gccggcgagg
    26941 tgcttgtagt agtgtttctg aacggtgccg tgcgccgcct cgaattcgta gtagccgtga
    27001 ggcgacacga ggacggacgt catcatggcg aggctgccga aggccgtagc caccatgtcg
    27061 gacatgacgt ccccgtcgta gttcttgagc gcccacagga cgcctccctc ggagcggatg
    27121 atccgggcga ccgcgtcgtc gatgagcgtg aagaaatatt cgatccccgc ctgccggaat
    27181 cggtccttcc agcgcgcctc gtattcccgg tcgaagatct gacggaatcg ggcgtcgtag
    27241 accttggaga tggtgtcctt ggtgctgaac cacaggtcga tcttctggtc cagggcgtag
    27301 gtgaagcagg cctgcgcgaa gctctcgatg gaggcgtcgg tattgtgcgt cccctgaacg
    27361 atgccgggac cggagaaggc gtgaatcgtc cgggtcgtcg cggggccgcc gtccgaggga
    27421 tgaaacacga tctccgtccg gcccgcgccc ggtacggcga gttcgacgcc gctgtagacg
    27481 tcgccgtagg cgtggcggcc gatcgtgatg ggtttgcgcc aggtcgtgac caaggggcgg
    27541 atgttccgga cgaggatcgg ggtgcggaac accgtgccgt cgatcatcgc ccggatcgtg
    27601 ccgttggggc tcttccaggc ctttttcagg ccgtattcct tcacccggtc ttccgtcggt
    27661 gtgatggtgg cgcacttgac cccgacgccg tatttcatga tcgcccgggc ggcctccacc
    27721 gtgaccgcat cgtccgtgtc gtcccggtgc ttgacgtgga gatcgtaggc cgcaaggtcc
    27781 atttcgagga agggcaatag caggtggtcc ttcaccatcc gccacatgat gcgcgtcatc
    27841 tcgtcgccat ccatctcgac gacggttgtt ttcacctgga tctttttcac caaagtcatc
    27901 ggttcaactc ctgtgttttc cttctttcag gatgtgcagc ttgcgttcaa ggcgccgccg
    27961 gcgaggatca acgcccgctg gcgcgtcgag agggtatgct tcacggcgaa cgtcaggtgt
    28021 ttcgtccggt tttccaccgt gagggtttct ccgccctcca gggcggcgcg cacgttcggg
    28081 attgtgagcc ggtccccctg cgcgatccgg tcgtagtcgg cctcgtccgc gaagaggagc
    28141 ggcaggatgc cgaaattgac caggttggtg gcgtggatcc gctcgaagga tttggcgatc
    28201 acggccttca ccccgagata catggggcag atcgccgcgt gctcgcggga cgatccctgg
    28261 ccgtaggaca gccccgccac gatgatgttg tggcggcctt cgtcgcggat gcgggcggcg
    28321 cgggcgggaa aggtagggtc gaggatttcg aagacgaatt tggcgtagcg gggaatgttg
    28381 gagcggtatt tcatgcgcgc tcccgccggg atgatgtggt cggtggtgat cttgtcgccc
    28441 accttgaggg tggtctcgcc gtcgagcgcc tccggcaggg gagtgttggc cggcggggcg
    28501 ccgatgttcg gaccgcgcaa gatggggacg gagtcggggt tcatgacgcc gtccggcttc
    28561 tcgatgaggc ggtcgtcgat ataaaaccgc tccggcatgc cgacgtccgg gtaggtcata
    28621 tcgagatcgc gcggatcggt caggcgaccg gtcaggacgg ccgcggcggc tgtctccgga
    28681 ctgacgaggt aaatccgagc gtccttcgtg ccggaacggc cgagaaagtt ccgattcgag
    28741 gtgcgcaggg aaacgccgcc ggaggagggg ctctggctgt tgccgatgca gaagccgcag
    28801 gcgctttcca tcagacgggc tcccgccccg atcaggtccg cgagatagcc gtcccggagc
    28861 aggttttcca ccacttgccg ggaaccgggg gcgacgatga agctgatatc gggatggacg
    28921 gttcgccccc ggaggatccg ggcgacggtg gccaggtcct tgtaggaggc gttcgtacaa
    28981 ctgccgatgc acacctgctg aacggcgagg gggccgatgt cgcggacggt acacacctcg
    29041 tcggggccgt gcgggcaggc ggccagcgga acgagtgcgg acaggtcgat gtccaccgtg
    29101 cgttcgaaga ccgcgtcggc atcggcggcg agcggaatcc agtccttctc ccgtccctgg
    29161 gcccggagaa aggcccgcgt catcccgtca ctggggaaga gagaggtcgt cactccgcat
    29221 tcggcgccca tattcgcgat ggtcgcccgt tccggaacgg tcagggcctc aatcccggga
    29281 ccggcgtact cgaagacgca gccgacgttg cccttcgtcg tgaagtgctg caggatgcgc
    29341 aggacgatgt ccttggcgga aacccagggc ggcaaggcgc ccaggaggtt gatgcggacg
    29401 acccgggggc aggccaggta aaagggctca cccgccatcg cttgggcgac gtcgagaccg
    29461 cccgcgccga tggcgaacag gccgaggccg ccggcggtcg gggtgtgact gtccgatccg
    29521 atcagggtgc ggcccggctt gccgaagcgc tccaggtgga cctggtgaca gataccgttt
    29581 ccggcgcggg acagatagag accgtatttc cgggcgacgc tcgtcagata tcggtgatcg
    29641 tccgcgtttt cgaaaccgat ctgcacggtg ttgtggtcga tgtagctgac ggaaagctcc
    29701 gtccgaacgc gggcgcagcg catggcctcg aactggaggt aggccatcgt cccggtggcg
    29761 tcctgcgtca gggtctggtc gatccggatg gcgatttcct ctccggcctc gaggcggccg
    29821 aaggccagat gggattccag gatcttctgt gtgagcgttg cggccatgcc atggttacct
    29881 gaatgaaggg ctaagggttg aaggttaaga atccgggacc gccgatggat cttcccggcc
    29941 ggtcatacaa aacatgcggt gcacccgggg gtacgccgca tggggggctg tcgattcccg
    30001 ttgcgcccgg cagtttttgg attcgggaaa cggcccggtt cctatcatat tcgctgtcga
    30061 ttgacaacaa cattcccccg cgcctccccg tgtcggtacg gaggtttctt ccggacgaag
    30121 gggacgaaaa ggcaaaaaaa atgggacgag agactcgtcc cagggttcgt gcattcgctc
    30181 aggaccatta agcgccgtcg tccaggccct tgagacgagt ggccttgacg aaatgcttgc
    30241 tgtaataggg ttcgtcaaga ctgttgaccc ggaccatttt cgatcgaccg gaggcggcgt
    30301 gaacgaactc gttgttgccg atgtagatgc cgacgtggcc gatggggcgg cgggtgtgga
    30361 agaagacgag atcaccctcc acaaggtccc cgcgggctac atgttttccg acgcgcgact
    30421 gttcgtgggc cgtccggggc agggagatgt cgaaaaacga ataaatcttc tttacaaagg
    30481 ccgagcagtc gagcccacga accgaattcc cgcccaggcg atagggggcg cccatgaagc
    30541 cctttgcgac cttgaccagg agttcccgct cgctgggatt gttccatttc ccgaggagtt
    30601 cggagcttgc gagcttgtcc ttttcaacgg cggcccagtc ttcctcgcgg acgggaccga
    30661 ggtcttcttc cgcgtctaat tcgtcgccgc cgcggagggc gacgtctggc gaggcctgtg
    30721 tgccgctgct gccgctttcc tccggtaggc gcggggccga cagggcgagt ctctggcccg
    30781 gcttgatcag atgggtgcgc agatggttca gacgcgcgag cgttttcact gagacgccgg
    30841 tcttcagggc gatcttccgc agactgtccc cccgtttgac cacgtacacc cgggccgact
    30901 tcgaattccg cgaagcggtt gaggcagttt tcttttgttt cgatacgctg gacttcgtca
    30961 gccgggccat ttgcgttccg gggacgaaga ggacctgacc gggtcgaagg accgttccct
    31021 tcatgtcgtt agcggccttg agatcggcaa tgctgacatg gaaggttcgg gatatcttgg
    31081 agagcgaatc gccgcgcttg accgtgtatg aaggttttgc cagagccgtc tgacacaaga
    31141 tgaatgacga gaacaggact gctagcccca tccatgggaa aatccgccgt ttcatcatgg
    31201 tatacctcct cttgggtagt cgggggttgc cccattttgc caacggtgca cagattttga
    31261 caatggatga caaactgcac acaaaaagtt gacgacctgt aacggatatt tcagggaatg
    31321 tcaaaacttt ttttacccac agcgatgatt ttcgaggcga ttttacgcat ccatctgtaa
    31381 aaatgctttt aaaatccgtt gttatggggt atgaggcgcg ggaggtaaaa aaacgttccg
    31441 ggaatgcgtc gatgttgaaa tgggttagga taatcgtctt ggtgaccgtg gcggcgcttg
    31501 ccgtcaacgt ttcgtattac ttcatttttc ccgatgtttc tcaattgcag cggaagaatc
    31561 cccagaaaac cgccttcatg aaataccggg aggcggagtg gcgggagaag ggccaccgcc
    31621 gcaagattcg tcagcaatgg gtcccctggt cgcgtatctc accgtatgtg gccaaggccg
    31681 ttatcatcgc cgaggacgac aaattctggc gtcacgaggg gttcgatttc aaggcgatgg
    31741 agaaggctct gaaaaaggat ctgcaaaaaa agcggttcaa ggtgggtggc agtaccatca
    31801 gccagcaact cgcgaaaaat ctttatcttt ccccgtccaa gaatcccgtc cgcaaggtca
    31861 aggaggccat cctgacctgg cggctggaga accggctttc caaacgccgg atcatcgaac
    31921 tctacctgaa tgtcgcggaa tggggggagg gaatcttcgg catcgaggcg gcggcccggc
    31981 gttacttcgg aaaacctgcg gcggccctca cggcccggga ggcggcgcaa ctggcggccg
    32041 tcctgcccaa tccccggcgg ttcaaaccga cggggggatc gcgctacgtg acctaccgct
    32101 cggagcggat ctaccagatc atggtccgcc ggggcattgt cattcccgag ttcgaggagc
    32161 tgttgacgga accggaggaa gcggcgacgg agggatggct ggatctcgac gttccggatc
    32221 cactttcccc gccggaaaag gatgaggccc ctcccggggc gcctgtcgag acggggccgc
    32281 agacgggtga caaggaacag gaaggcttgg ctccgcctcc cccgtcggag cctgccggcc
    32341 catctgcctg acccacgccc caggtcaagc gaaacggcgg ccggggttac cccggccgcc
    32401 gttgtttccc gcccgtgatt caagtctgaa tcagagcacc tccaaggcga cggccaacga
    32461 tacgcctcct ccgccgcaca gtgtggcgag cccttgggtc ttgccgcgtt tcttgagggc
    32521 gtggatcagg gtcaccatga tgcggcaccc cgtggcgccg accggatggc ccagtgcgca
    32581 gccggagccg ttgacgttcg tgatctggcg gttcagtccg agctcgcgct cgcagcccag
    32641 gtactgggag gcgaaggcct cgttgagctc gatcaggtcg aaatcgtcta tcttcatgcc
    32701 cgacctcttc agcagatcct gaacggccgg caccggggag aggcccatga cggaggggtg
    32761 gcagccccca cgtccgacgg ccgtgatgcg ggccaggggc ttcaggccga gcgcctgcgc
    32821 cttttccgcg gacatcagca ggacggccgc ggcgccgtcg ttgatgccgg aagagttccc
    32881 cgccgtgacc ttgccgatct tggggatgaa ggccggcggc agcgcggcga ggtccgccag
    32941 ggtgagcccc ggccggaaat gctcatcgcg gtcgaagaac ttcggcggtt ttcccttctt
    33001 ctgggggatc tccaccggta cgatctcggc ggcgaagtcg ccctcgaggg tcgcccgttc
    33061 agcgttgttg tggctgcgta gggcgacctc gtcgatctcc tcccggctca ggttgtgctt
    33121 ttgggcgatg agttccgccg tgatgcccat gatgtagggt ttgccgcgga aaaggtcgac
    33181 gatcggtcct tccttgatgg ggcccttctc caggccgcga atcacgtggg aaccggcatg
    33241 gagaccgtgg atgaggttat ccacgaagac ctggtcctga agacggcatc cccagcgcgc
    33301 ggcgggaacg gaatagggcg ccccggacat gtgttccaca ccaccggcca ggacgatctc
    33361 ggcgagtccg gcctggatcc aggccatgga agagacgacc gcctccatgg cggagatgca
    33421 gacgcggttg accgttacgg ccgtggtcgt ctccggaatg ccggccagaa gcgccgcgat
    33481 tcgcgtgacg ttcaaggtat cgaccggctc catgcagcag ccgaagcgca cgtcctcgat
    33541 caaggcgggg tcgatgccgg cccgggcgat ggcctccttc atcacgacac tcgccagggt
    33601 ggcgctgttg gagcctttca ggctgccccc gaacgtgccg atggcggtcc gacatgcaga
    33661 tacgataacg acatccttca tcctgttata tcctccttaa ggggtgtgat tcgagggctt
    33721 accgccctcg ttgggatcag ggtcggggcc atgaaaaaag gctgtaaaac cgatgccggt
    33781 ctcacagcct ggatcttctg gctccccagc gcggactcga accacggacc cgatggttaa
    33841 cagccatctg ctctaccgac tgagctactg gggaatcggt ttagggcgag tctcatagta
    33901 caagcctccc ggaaagtcaa caggcgaatt gcatggggga gagattttgt gaacgccttg
    33961 aaacggttga ccgcacctga ggccccccag ccgaatgtcg gtcgccctgc tttttgtctt
    34021 gactatgggc aagcccttcg ataggttgca cgatcatatt caaaacatga acgaatccgg
    34081 agagggaggt gacggaaaag acttaacctg acaaccgctg ttgccgcagc cgcaagggaa
    34141 ggagcccact catggaggca tcagaacatc cgcacggagc acaagacttc aaggtgtcga
    34201 gaggctacgc gtattatgtt ttcctgctgc tcttcctcct ttaccttttc aattatgtag
    34261 atcggatggt cgtggccgcg atcttcccct atttgaaggc cgactgggga ttgaccgata
    34321 cccagtgcgg ctggtttgcc tccatcgtca ccctgatgat gacggtgttt gtctttccgg
    34381 tctccctgct ggtggaccgg tggagcagaa aaaaggccat cggtattatg gccgtgctgt
    34441 ggagtttcgg cgcggccgca tgcgccttca cccgcaactt cactcagctt ttcacgatgc
    34501 gatccctggt gggcgtgggc gaggcggcct atacctccgg cggccatgcc atgatcgccg
    34561 cctatttccc tgaagaaaaa cgggccacca tgaacggcct ttttacggct gccataccca
    34621 tgggtacggc catcggcgtc atcttgggcg gtgtgatcgc cgaaaccttg ggctggcgat
    34681 acgccttcgg catcctcgcc gtccccggcc tgatcgtggc cattctcttc ttctgggtga
    34741 aggattacaa gaccgtgcag atcgtgagaa caaccgggga tggcgcacag gccgtccggg
    34801 tgaagatgaa gtttgccgat atcgtgaggg agttcctgca tacgccttct gtgctgttca
    34861 cctatctggg atacgtggga aacacctttg tcaccaccgg cttgatgaac tggctcccct
    34921 cctactacca ccgcgtggac ggcctgccca tggacaaggc cgggctcaag acatccatcg
    34981 ttttcctcct ggccatcttc ggcgcgccca tcggcggcta catcacggac aggatccgga
    35041 agaggttcct gaacgcccgc atgaccatcc ctgccgtcac ctcgtttctg acggcgcttt
    35101 tcctttttat cggcttctcc ttcctggagg ggagggcgca atacctcttc ctgattttct
    35161 tcgggtttac ggccccgatg ttcgccgccg gcggttccgc ggtgacccag gacgtggtgc
    35221 accccggcct ccgcgcgata tcgtacagcc tggcccagtt cttcatgatg ctcctggggt
    35281 attcattgag ccccattttc atcgggttta tctcggaccg ctatgatttg ctcacggcct
    35341 tcaagttcct gccgctgttc gccctgttcg gtggcgccgc atttttcgtg ggatcgttct
    35401 tctatgtgag agacctccac aaggtggaaa aggttagact ggaaggatga tcttgcatcg
    35461 catcgtgtga aataaagaca tgcggtgagc tgggaagggg gttgttgtga aaccgaatgt
    35521 catcgttatc catcccgggg acaacgtcgc cgtcgccctg gaggatattc ccgccggagc
    35581 ggccgtttac ctccccgacg gtcaggtctt tgccgcgctc acggcgattc cgtacagcca
    35641 caaggttgcc ttggccgatt tggttgccgg tgccgtggtc gtcaagtatg gcgagacgat
    35701 tggggaggtc acggaggacg ttcgtcgggg cgactggatt cacgtccaca acctggatat
    35761 cgaagagcaa aaggaaaact cgtagatgga aacgtttctg ggatacgcca ggccggacgg
    35821 ttcggtgggg atccggaacc acgtcgcggt tatcccgtcg gtcgcctgtg ccaacggggt
    35881 ggtggctgcc atcgcccggg tcctccccga cgttgtgccc ctctaccacg gccacggctg
    35941 cggccgggcc gtcgagatcc cgctccacac ccgcgtgctg gtcaacctgg gccggcaccc
    36001 gaattgcgcc gcggtgctcg tcgtggggct gggatgcgag gtcctcaaag ccgagggcat
    36061 cgcccgcgcc attgccgaaa cgggcaagcc ggtcgactat ttcaacattc aggaatgcgg
    36121 aggatcacgt cggtcggcga cgaagggcat tgaaatcgcg gccgagatgc ttcgcgcggc
    36181 cggccggtgc gaacgtcgcc cctttccgct ctcccggctc attctcggcc tcgaatgcgg
    36241 cggctccgac gccttctccg gtatcacggc caaccccgct gtcggcctcg cctcggactg
    36301 gctcgtcgac cggggaggca ccgttcttct gacggaggat acggagatga tcgggacgag
    36361 cccgatcctg gagcggcgcg caaagacccc cgcggtcgcg ggagagatca aaacaatgat
    36421 tgccgacgcc gaacggctga cccacgacct cctgggacct ctcgcgccct tcgtcatcgc
    36481 cccgggcaac atggacggcg gcatgagttc gatccgggag aagtcgctgg ggtgcatcgt
    36541 caaggccggc agccgcacga tcacccaggt gaccgactat ggggaggtcc cggccgaaaa
    36601 aggagttgtc ctcatgcgcg gtcccgggta tgatacggaa tccatcgccg gggtcgccgc
    36661 cgcgggcgcc cagttcgtcc tctttacgac ggggcgcggt aatccgatcg gctttcccac
    36721 cgtccccgtc ctcaaggtgg ccagcacggc gaaactgtac cgggcgatgg aggacgacat
    36781 ggatatcaac gccggggccg ttctcgaggg gaaacgcttg gcggcggtcg gcgacgagat
    36841 tgtcgccctc atccgcgaag tcgcggacgg acgcaaaacc aaggcggaaa tcaaccggca
    36901 ggacggcatt gtctgcctct acacgcgcca tccggccttt tgacggttga gaagggaggg
    36961 gtctgcatga ggagggtgtc tctgttcgtt ccgtgtctgg tcgatctgct cctgcccgag
    37021 atcggagaag ccgcctttgc gctgctgaga cgcctcggcg tcgaggtcgt gtaccacgag
    37081 gctcagacct gctgcgggca gccggcgatc aatgccgggt accttccgca ggcgcgcaag
    37141 gtcgcccggc acttcctcaa ggtctttggc gaggacgagg cgatcgtctg ccccgccgga
    37201 tcgtgtgtcg tcacggtcaa gcaccactac ccggagctgt ttgccgacga accggactgg
    37261 cggacccggg cggagtccct ggccccgcgg gtctacgagc tgtcccagta ccttgtcgat
    37321 gtgctcggcg tggaggatgt gggggcgacg ttcgggggga agatcgccta tcacgaggcc
    37381 tgccacgtcc tgcgggggct cggcgtttct gaacagccga aaaagctggt tcgggccgtg
    37441 accggcgcgg aactcgtgcc cctgaacgcc gccgacgttt gctgtggctt tggaggagag
    37501 ttttccctgc aatatccgga tatttcagaa gtcctggtcg cggagaagac cgaaaatttc
    37561 atcaacagcc aggccgatgc cctcgtcctg tgtgagccgg gatgtttcct gaatatctcc
    37621 ggatacgtgc accgtcatca tccggaaaag aaggtcttcc atctggccac gcttctcgcg
    37681 ggcaacggaa aggggggggg cgcatgaaga tccggacgga acagtttgtc gagatcgccc
    37741 gggaggagat gaaaaacacg cgctcgaagg cctttttggg cctcctgccg ccggtgatca
    37801 acgccatgcg gcagatatcg ctcgcgacct ttcccgatcc ggacgccgct caggcgtacg
    37861 gcgcggcgat ccgcggggag gtggtgaccc gtcttcccga actgctggag gaattcgaga
    37921 ggaacgcgac cgcgcggggg acgaaggtcg tctgggcgcg gacggccgcg gaggccaacg
    37981 ctttcgtcct cgatctggcc aaaaagaagg gcgtcgccta cgtcaccaag ggaaaatcca
    38041 tgctgagcga ggagatcggg ctcaacgatg ccctcatcga caacggcgtc gacgcaatcg
    38101 agaccgatct cggcgagttc atcattcagt tgctcaagcg cccccccttc cacctgatcg
    38161 gtcccgccgt caacatcgcg gtggaggaga tccgcgacct ctttatggaa aaaggcatcc
    38221 tggcggagcc gacggaagat cccgtcgcgc tcggctatgc cgcccggctt tatctgcggg
    38281 acaagtttca ccgtctggag atgggggtga cgggggtgaa catggccgtg gcggagacgg
    38341 ggacgatcgt caacgtggag aacgagggca acatccgctt cgccaagtcg tccccccgga
    38401 cgcaggtttc catcatgtcc ctcgaaaagg tcgtgccgac gatggccgac gccctccaca
    38461 tgatccgcct cctgtgccgg aacggcacgg gccagaagat ggcgtcctac atctccttcg
    38521 ataccggacc gcgggcggcc ggggagatcg acgggccgga ggaggtctat tgcgtgatcg
    38581 tcgacaacgg tcgggcggac ctctacgacg atcccgtcgc ccgggacgtc ttccgttgca
    38641 tccgctgcgg ggcctgcgcc aacatctgtc ccgtgtatac gaagatcggc ggctatccct
    38701 acggctgggc ctacacgggc ccgatggggc agctcctgac cccgctcctc ctcggcttct
    38761 ccgggacgtc ggacctcttt cgcgcctgca ccctctgtgg ggtctgcaag agcgtctgcc
    38821 cggccggggt ggaccacccc cggatgcttc tttactaccg tgcccgggat gccgcgggcg
    38881 acccgagact gagatcccgg gggcggccct tgaaggaacg gacgctttac gacctcttcg
    38941 cctggggcgc cacgcatccg cggttgtgga ggcagggggc aaagcgcatg cgcccccgag
    39001 tcaaccgcgg cgcgcgggac gggtacctga gccggtccgt ggaaccgctc gacgggtggt
    39061 tccggagccg ggacctgccg gagatggcgg aaaagacgtt tcatgaacgg tggaaggagc
    39121 gggaagggca taaggcgaaa gcctgacgcc tcaaacgaag gaggggagca tgccgtacgc
    39181 tgcgagagag gacatcctcg ggaagctcaa gaaggcggac cggcgggagg aggcgccggc
    39241 gagacccccg ttgccgcccc tgacggaggt cggctggggc acggaacagc tgatcgaaaa
    39301 gttcacggag aatctcgccc tccagacggg agtcgtccac cgggtcgccg accgcgcggg
    39361 cgccctcgaa aaactgacgg agatcgcgca ggccgagggc tggaaggcgg tcatgatcgc
    39421 aaccgacgag gtcctggcac cgctcgacct cgcggcctgg ggccggacga acgggattga
    39481 tgtcctgaag gcggcggatt tcacggcccg ggacgccttc aaagacgccg tcttcgatca
    39541 ggtggacgcc gggatcacgg gcgtggactg ggccgttgcc gagaccggca cggtcgccct
    39601 cgtccacgac gagcggcaac cgcgtctggt ctcgctcgcg ccgatcctcc atgtcgtggt
    39661 cgtcccggtg gaccggattc tgccggtcta cgagcaggcc ctggagcgga tcctcgcggc
    39721 ccggggtggc atgccggcgc acctgaacct gatcacgggc ccgagcatga cggcggacat
    39781 ccaggcgacg cccttcaagg gaatgcacgg cccccggcgc gtcatcgccc tcctgatcgg
    39841 gtaggccggg caggctttag gcaattgatt aaaaatgcgt gcaatcgttc gatatgaatt
    39901 gattgatgat cttgtgaggc aaacatgaaa agtgtctggg tgccgtcacg gcgctggtat
    39961 gagaacgagg agcgggagct gacgttcccg gaccgctggg gggtggacaa cctgacctcc
    40021 ccggggctgg agaagcccgg actcacgccg gaagaggtcg cggcgaagat cgcgcgcccc
    40081 gtcgcggggc cgctcctgga ggaactcgcc cggggcaaaa aacaggccgt catcgtcttc
    40141 gacgacatga cgcgcccgac cccggtgaag gaggtcgcga cgaccatctt ggatgccctg
    40201 cgccgggccg ggatgcagcg ggaccagatc cggttcctct gggcactggg gagtcacgcc
    40261 gcctacgaca tgatcgccgc ccgcaagaaa ctgggggacg acatcgtcga gcgctacgcc
    40321 gtctataacc acgacgcctt ccagaactgc gtccgggtgg ggcggacgcc gaccggcgtg
    40381 gagctgtggt tcaaccggga atacctggcc tgtgacctga aaatcgggat cggctgcatc
    40441 accgcccacg tccacgtggg gttcggcggc ggtgcgaaga tcgtcctgcc cggcgtggcg
    40501 gggatcgaaa cgatcaacca gtttcacaac cagcagttcc gggatttcgc ccggacgggt
    40561 ctgggcaact tcgacaacaa catcatgcgg gccgaatgcg acgcggcggg agatctcgcc
    40621 ggcctcgact tcaaggtgga ctgcctcatc aaccgacgcg gcgagatcgc gaacctctac
    40681 gccgggccgt tccgcgagac gcacgccctc ggtgccgagg agggccgggt gcactacggt
    40741 atcccctgcg cggtgggcta cgacgtcacg gtctgcaatg cctacggaaa ggcgaacgaa
    40801 tccgcgatcg cactggtgtt cacgctggcg acgatgaagc cggggcaggg caccggggtg
    40861 ctcgtctgcg acgccccgga gggccaggtt ccccattacg tcatgcgtgc ctgggggtct
    40921 gactacggcg gacgccacta ccaggcccgc gcgaaggggt tcgtgggcct gctcatgaag
    40981 aagttgatcg tcttcaatcc gcagcccgac cggacgagcc tggacctcgt ctgccacgcg
    41041 gatgatgccg tcgtcgtcaa gacctgggac gaggttcggt ccatcctgga ggccgattat
    41101 ccgggcgagg cgaaggtggc cgtggtgcag gacggaacga tgcagtacat cctgccgccg
    41161 ggaaatgggt gaaaacgacg cgttttggcc attcgagggg tttgacaaca accgaaagag
    41221 ggaggtcggg ttcatatgga aatgacgatc atggggcaga aagagctcca ggaacgggtg
    41281 caccgggcgc ggctctgcac gggatgcggg gcctgcgtca acctctgtcc gtatcaggtg
    41341 ttctacaacg accagacgat cgttctggat gcctgcgaca tcaaggaggg ccgctgtttc
    41401 gccttctgtc cccggacgcc cacggacctc gatgcgatgc ggaggcgctt tttcgccccc
    41461 ggaaacctga cgccggagat cggggcggtg aaggggtacc tgattgcccg cgccgccgat
    41521 gcggccgtcc gaaggggcgc ccagcacggg gggaccgtca gcgccctgat ggcgctcgcc
    41581 ctcgatgaag gtctgatcga tacggccatc gttgccgagg gcgcggaccg ctttctccag
    41641 cagggcgtcg ccgtcggaga cggggcggag gtcctgaagc ggggcaagag caagttcatt
    41701 gcggcggcga cggtggccgc cttcaaccgg gcggccaagg gcccggccga agccataggc
    41761 gtggtcgcca cgccctgcca ggtcttcgcc ctcgccaaga tgcggatgaa gcccctgccg
    41821 gagctggaca acaatatcga caagctgaag ctcgtcatcg ggcttttctg cggctgggcc
    41881 ttggactggc ggaaattcac cgccctgctc gcggcccgaa cggccctcga ggacatcacc
    41941 gggatggaca ttccccaggg ccgtcgcctg gtcgtcgtct acacccggaa cggcgccctc
    42001 gaaatcccgt gggacgaggt cgcgcccatt gcccgggagg cctgcggggt ctgcatcgac
    42061 acgacggccg agttcgccga tctttccgtg ggctccgccc ggatcggcga gacgtgggag
    42121 acgacccgga cctggaacca ggtcatcgtc cggacggagg cgggtcaggc cctgatcgac
    42181 ctggcgcgtc gcaagggcgt tctcgaattc cgcgaccccc ccgccggggt gctcgacgaa
    42241 ctcaaggccg cagccaggga caagaagaag gcgtccctgg agaagatcat cgccaggacg
    42301 ggaaaccccg aggacctgat ctacgtggat cgccgggacc ccgtcatccg cgccatcctt
    42361 gattagaaaa accattcctg taacgacacg gaggacaaga tgtcgaaact gatcgatccg
    42421 cgcgagggcg tccgggtgct cctcatgggc aatgaggcca ttgtccgggg cgccctggag
    42481 gccggtgtca acgtggcggc gggctatccc ggcacgccgt cgtccgaaat cgtccagaac
    42541 ctggcggacg tggcggggga gcgcaacctc tacgtcgagt ggtccgtcaa cgagaaggtg
    42601 gccatggagg tggcggccgc ggcctccttc gcgaagctcc gctccctgtg cgccatgaag
    42661 cagaacgggg tgaacgtcgc ctccgacttt ctgctccatc tgacggcggc cggcacgcgg
    42721 ggcggaatgg tgcttgtcac ctgcgacgac cccggggcgc tctccagcgt caacgagggg
    42781 gagtcacgcc acttcgcgcg catggtggag gtgcccctcc tggagcccgg cgatttccag
    42841 gaagcgaagg acatgacgaa atgggccttc gagctctccg aggagatccg gaacctcgtc
    42901 gtcctgagga gcgtgacccg catgtcccac gcgagcggca acgtgaccct ggggcggctc
    42961 tccgacgcgc ggccgaaggc ggagttcaag tacaacggct tcatcctcga ccccctggag
    43021 gggcccgtcg tcagcgcgcc ggtgccctac aaacacaccc tccaacagga aaagttgaaa
    43081 agggtccggg agatcttcga agagagcccc ttcaacacct acgccggccc ggagaaaccg
    43141 gacctgctcg tcgtcacgag cagtgcctgc accctgtaca gccgggaggc gatcgacgtc
    43201 ctcggcgtga aagaccgggt gggtcttttc aagctgggga ccacctggcc cctgccgccc
    43261 gaactgctca agaaacgtct cgcgtcgacg gaccaggtcc tcatcgtgga ggaggtcatc
    43321 ccctttctgg aggagaacgt gaagatcctg gccgcggaaa ccgcacgcga gatcggcgtc
    43381 aagaccttct ttggaaagaa cgacgggacg ctcccctcca ttggcgagct gaatcccgac
    43441 ctcgttgcgg tggcgatcgc aaaaatcctg gggatcgacc acgtcgccat gccgcctgaa
    43501 tacgaaaaga aggcgacgct catgagtttc gcaaaggccc ccaaccgtga gctgaccttc
    43561 tgtcccggct gtcctcatcg ggcttctttc tgggcgatcc accacgccct gctcatggac
    43621 aaccggctgg ggttcgtctg cggcgatatc ggctgctatt cgatggcggc cctcccgtgc
    43681 gggttcagca ccctgaagac ccttcacgcc atgggatcgg gcaccggggt cgcgagcggg
    43741 ttcggccagc tccaccgctt cggcatggaa cagcccgcga tgacggtctg cggcgactcc
    43801 accttcttcg cctccgtcat gccggccctg gtgaacgcga tccaccaccg cgccgagctg
    43861 accgtggtcg ttctcgacaa cagcggcacc gccatgacgg gcttccagcc ccatccggga
    43921 ctcaaggtga acgccatggg cggggacgtt cccgccctgg acatcgcgcg gatctgcgcg
    43981 gcgatggggg caaaggtgga ggtccgggac ccctttcaga tcgaagagac ccgcgagacc
    44041 ctgatccgcc tcctcgacca gaagggcgtc aaggttctga tcatgcgcca gatgtgcgcg
    44101 cttagtccgg agaagcgggc gaagaaaaat tatgaggtga aggtggacga ggccttgtgc
    44161 ctgggcgaga cctgcggctg caaccgcctc tgcacgcgcg tcttccgctg tccggcgctc
    44221 gtgtgggaca aggagcggaa gaaggcgagg gtggacgagg tcctgtgcgc cggctgcggc
    44281 gtctgcgcgc tcatctgccc ggcgggggcg atccggaaag aggaggtggc ttgaacgatg
    44341 ggaacgcaaa ccttaccgaa tgatcccttc aacatcatca ttaccggcgt gggcggacag
    44401 gggaacgtcc tggcctcgcg cgtgctcgcc aacatgcttg tccgccgggg gtacgacgtt
    44461 accatcggcg agaccttcgg cgcctcccag cgcggcggtt cggtcatgag ccacatccgg
    44521 gtctcgaccc ggggcgcctg gagcccccag atccccaagg ggaaggcgga catggtcgtt
    44581 tccctggagc cggtggaggc gatccgggtc atgaaggact acggcaacga agaggtccgg
    44641 atcctcgtca acgaccggcc catctatccg gtgggcgtga tcgccgggga gctcaactat
    44701 ccggcgctcg acgagatcgc cggcgccctg aaggacctgt ccaagcatgt ctggttcatc
    44761 gacgcgaccg aggaggcgat ccggctcggc aatccgatcc tgggcaacgt catcatgatc
    44821 ggcgccgcct gcggcctcgg catcctgccc gtgggccgcg ccgagttcgc cgaggtcatg
    44881 aaggagagcc tcgcggccga caagctcgag atcaacctgg cggcgttcga tctcggggcc
    44941 gccaagcccg ccgccgcggc atgaccgtcg cgacgcgaac gggcaccgcg gggaggggtg
    45001 tttttccgtc atggccaaac tgacgctcaa ggagtggctc gcgcgggtcg agggcgaccg
    45061 gcgcttcatg gagaacgtcc gggcgatccg ccggatcccc gcccgcgacg gggtctacgc
    45121 cccgtatccg gactgggtgc acccctcgct cgcgaaggtc ctcgcggcgc gggggatggg
    45181 gcggctctac agccaccagg ccgaggcggt cgcgtgcgtc cgccgggggg aaagcgtcgt
    45241 ggtcgtcacg ccgacggcaa gcggcaagac cctctgttac aacctgccgg tcctgcagcg
    45301 catcctcgaa gagccggaaa cccgcgccct gtacctgttc ccgaccaagg cgctggccca
    45361 ggaccagatg cacgaggtcc acggactgat cggcgggctg ggtgcggaca tcaaaaccta
    45421 cacctacgac ggggacaccc cggacgacgc ccgccgggcg atccgccggc aggggcacgt
    45481 cgtcgtgacc aatcccgaca tgctccacgc cgggatcctg ccccaccaca cgaagtggca
    45541 gaagctcttt gcgaatctcc gctacgtcgt catcgacgaa ctccacatct accggggggt
    45601 tttcggatcc catctgacca acgtcctgcg gcgcttggtc aggatcgccc gcttctacgg
    45661 ggccgacccc gtcttcgtct gctgctccgc cacggtggca aacccgcgcg agcacgccga
    45721 gcgcctcctg gagcgtcccg tcgcgctcat cgacgagagc ggggcgccgg ccgcggcgaa
    45781 gaccttcatt ctctacaacc cccccatcgt gaaccgggaa ctcggcattc gccagtccgc
    45841 cctgacgccg gcccggaaga tggcgggcga cctcatcgag aacggcgtgc agacgatcgt
    45901 cttcgcgacg agccgcctga acgttgaggt tctgacgaag tacctcaagg accgcttcgc
    45961 gaagggaaag cccgtcgacg cgcagttcgt gacgggctac cgagggggct atttgccgaa
    46021 tctccgaaga cagatcgagg ccggcctgcg cagtcgcgag gtcatgggcg tggtcgcgac
    46081 gaacgccctc gagctcggga tcgacatcgg cgacctggag gcggccatcc tctgcggcta
    46141 ccccggctct atcgccagca cctggcagca ggccgggcgc gcggggcggc ggacggggct
    46201 cagcgtcgcc gtccttatcg ccaagagcgc gccgatggat caattcatcg tggagaatcc
    46261 cgactatttc ttctcccgtt caccggagtt ctgtcgggtc aatccggaca acctcctgat
    46321 cctcctgcac cacatcaaga gcgcggcctt cgaactcccc ttcgagaagg gggaaaactt
    46381 cggcggtgag aatcttgagg aactgctcga ctatctggag gagaagggtg tgctgcaccg
    46441 cacgggcgaa cgctggcact ggtcggcgga gagctatccc gccgacgagg taagcctgcg
    46501 gagcgtcaac cccgagaacg tcgtggtcgt cgatgcgacc gaagcgggaa accaccgggt
    46561 catcgccgag gtggactggg acagcgcctt cacgaccgtc cacgacgacg cgatctacat
    46621 ggtggagtct cagcagtatt acgtggacaa gctcgacctg gagcgcaaga ccgcctacgt
    46681 ccacaaggtt gaggcggagt atttcaccga cgccatgacc tatacgaacg tccgggtcat
    46741 cgatgccttc gcgaagaagc cggggcggga ggttctggtc gagcacgggg aggtccaggt
    46801 ggtgagcaag gtcgtcggct acaagaagat caagttctac acctcggaga atctgggcta
    46861 cggcgatgtc aacctgccgg agagggacat gcacacgacc agctactggt tcacgattcc
    46921 ccgggaccgc ttgtcccgcc tgccgttcca tcaggcggag atcatcgacg gcctggcggg
    46981 gaccgcctac gccctgcacc atctggcggc gatgctcctg atggccgacc tccacgatct
    47041 cgaccgggcg atcggggata agagcgggga atggttcgtc cggacgggaa aacagggtcg
    47101 gacgatcacg gcctcccccg ccggggagga cggcgcgacc cccacgatgg acgccttcga
    47161 cccgacgatc ttcctgttcg acgcgtaccc cggtggaatc ggcttctccg acctcctctt
    47221 cgagcgccac gacctcctga tgcgcttagc ccgtcagctc atcgaaagct gtccctgtcc
    47281 aaacggctgc ccctcctgcg tcggtccggt ccttgaggtc ggaccgaagg ccaaggaggc
    47341 ggccctggcg attctggacc tgatcgagac gtagaaaaaa tcccggacgc cggcggagct
    47401 gcatttcttg gccgcgtcgt tgttcccccg tttcggagaa atgtcttgac ttctcgaaaa
    47461 atgaggctat ggtctaactt agagaatgta atcgacgctc ctcaaaataa tagtggttga
    47521 tcccctctgt ccatcgcgat aacacgaatg ttttttggcc ctgtctatta catgaggcag
    47581 ggttttttgt tggggcgggc cccgtgtatg gccgagaggt gaaaacgcat aaacgtccca
    47641 cgaggaaggt gatccatgaa tatccacatt ttagagacaa cgaacaattt catcaaggta
    47701 caggaaaacg tgtgggaaag cggctactgg aagctggacg aagcccaggc aaaaaagttg
    47761 gtcggcggag agatctactt tcacaagaaa agacaggagc cttccttcta cggcggcacc
    47821 atcctggcct atcgggttgc gccggaaggc gttcatgagg gcaggatcat cttcacgctc
    47881 aagcatctca ccgcatgcag aaatgtccga acggacaagt cgggatggtc gaagaacatg
    47941 aaggtcatcg atcctgaatc ctaggaaccc gccgacgggc ggatgtcccg tgaggcgcga
    48001 atcggacggg tcctcttgcc gaacgcccgg gtcgcggcgg aaggatcttc ggtgcgcaga
    48061 ccgaacctga ggacgccgcc aacctcatcc cagtataaat agcggttgaa cttgcccggg
    48121 agcgtggtat catgcggcca tgagtgtcag gggccgttta cagagactga caggcgagac
    48181 gaccaacggc ggcaacgatt cccgcgaggg atcgcgagca gaggacatct cggccctgcg
    48241 ggagcgaatc gaagccatcc tctcccggcg gcctgaggcg gggcagggtg gggtgtcgcc
    48301 gtccttccgc ggacgcggcg tggcattgga ggatctggtt ccgggtgtgg aaacggcgaa
    48361 cgcggccggg gtctttttct gcgcgtatca gaaggcgggg gagtccgtcc gtcacggggc
    48421 gcgatgcgtc cgggattggc tgtccctcga catggaacgg ctcgccgttc tggcgaacga
    48481 cgctgccctc aacaggcttg aggttgccca ggccctcttc ctcgatacgg aaacgacggg
    48541 actggcgggc ggagcgggga cggtgccctt tctcatcggc ctcggctggt tcgaggaggg
    48601 gggattcgcg acccgtcagc tctttgcccg tgattacgcc gaagagcgag cggcgctcgt
    48661 ccatctgcgg gaactggcag cggagcggcg gttcttggtc accttcaacg gcaaggcctt
    48721 cgacgtcaat ctcctggcgg cgcgcttcat catgaaccgc ctgccggatc ccctctccgg
    48781 actcccccac ctcgatcttc tccatcccgc ccggcgcctg ctttcccatc gcctgggcaa
    48841 ccgacgtctg ggggcgctgg aggcggagat cctgggattc gagcgggaag gggacatccc
    48901 cggaagcgaa atcccccggc ggtacttcga ctggctccgc aaccgcgacg ggcggctcat
    48961 ggccgacgtc ttcacgcaca accggctcga catcctgtcg atggcggccc tggccgcccg
    49021 cctgacggac atgatcgtgt ccgacggcgc gacctccgcg gatcgtccgg acgaccgcct
    49081 cgccgccgcg cgtttcctgc tcgcgcggga tcgtccggaa acggccgcac gcatgctcgc
    49141 gccgctttgc gcctgcgacc atccggacac ggcgcgggag gcggcgcgcg aattgtctct
    49201 gcttcacaag cgcgcaggcc attggacggc ggcggtcagg ctctgggagg agatggcccg
    49261 gcgggacccc ggagacgcct ttcccctcgt cgagctggcc aaatggtggg aacaccggcg
    49321 acacgatttc gatcgggccc tggcgctgac cgaccgcgcc ggcgccggcc gcctgtcggg
    49381 agacgaccgc gccgctcttg accgccgccg cgaccgtctg aaccggaaac aggcggcccg
    49441 ccccgcccgc gcgtaggcgc gcgcgcccgt tcccggtcgg attcagggga acccccaacc
    49501 ggccggccgc gtcggccgga gccggatttt ttctttgacg ggcggagggc gacgcagtat
    49561 atgggggccc cgatcggccg tggggaacgg cgaggggccg atccgtcatt tcgatgcagg
    49621 gtgaggaaaa ggaacatgcc gggaaacctg gcatcggtca attccatctt ggtacgctgc
    49681 ccgaactggg taggagacat tgtcatggcc accccggtct tctcctgcct cagggagaat
    49741 tttcccgaag cctccatcgt ggcctgcatc cggggatatg cccgtgggat catcgaagac
    49801 ggcccatggt tcgacgggat catcgcctgc aacgacaaaa cctttgccgg cttcaggcag
    49861 acggtaagcg agatccgccg gatccgtccg gacatggtga ttcttctgcc caactcgatc
    49921 cgctctctac tgacctcatg gctcggaggg gcaaaaaaga tctacgggta caagaggaac
    49981 ctcagggggc tgctccttgc cggaggaccg gcgcccgttc gcgacggggc cggaagggtg
    50041 aaggccctgc ccatgcaaac gtattacctg gagatatgcc gctcgctgga tctgacaccg
    50101 ccggacgatc cccggccgga gttgttcgta tcggcggagc tccaggccaa gggcgaaagt
    50161 ctcctgcgga agtacgggat cgcccccggt gaccgggtga tcggtctgaa tcccggggcg
    50221 agcttcgggt cctccaagtg ctggccggcc gaatacttcg ccgaactcgc cgggttgctg
    50281 aagcgcaggc tggacggcaa gatcatgctc tttgtcggcc cgggagaaga ggggatcgcc
    50341 gaggccatcg tcaaacattg ccgggtttcg gtaatcgata ccggaccgga caggatcgac
    50401 ctggccgccc tgaagcccct gatccaacgg tgcaacctgt tggtgaccaa cgacaccggt
    50461 ccaagacaca tcgccgttgc cttcggcgtg ccggtcgtcg tcatcatggg gcccaccgat
    50521 ccccggtaca catcggccaa cctggacaag tccgtcgtga tccggaaaac gctggactgc
    50581 tccccgtgtc ataaaaaggt ctgcccctgg gatcaccggt gcatgacgga gatccggccg
    50641 gaggaggtcc tgggggaggc gatcaaactg ctggagagat ccgatttgag atgagacctg
    50701 aattttacga gggcaaatgg gcgcgcctgc gcgagcgccc gacagcggag gcgctgaaac
    50761 ccctggccaa gcagatcgcc ctgtcgtttc tcgacgagta tttctacaac gatcggtacg
    50821 agggcggcta tatccgtctg ctgtgcgaga tggccacggc cttcgaggac gatgcgctca
    50881 atctgtccgg cgcgggcgcc ctgttcggca tcgtcatcga gagcctctgt gacgattttg
    50941 aagaactcca gaccgaaacc tacaaccggg tgatgtgcca ggtcgtcacc cattgccgca
    51001 ccctgcccgc cggggcctgg ctggaccggc ggctgaggga tttcggcatg gattcctatg
    51061 agtccctgtt tggccggatc gaaaccttcc gcaatcacag cgatgcgttc cggccggaca
    51121 tccggccccc gaaaaagatc ctgctcctgt cccgcgtgac catcggcgcg gacgtcgcca
    51181 ttacgagcgt gatgatccag cggctgaagc gcaccttccc ggaggcggag attcgggtca
    51241 tcggcggtgc gaagctcgac cagatcttcg gcggaaatcc cgatgtccac atcgatgtgt
    51301 tcgactactc ccggcggggg gggctgttcg aacgtttccg gggctgggag gccgtgctcg
    51361 aggggatcgg ccgggaggcg ccgccggacc tggcccggga ggtcatcctc gtggacccgg
    51421 actcccgcct gtctcaactc ggtgtgctgc cgctgatcgg ggacgatcag tacctctttt
    51481 tcaacagccg gggaaaggat tcctacccca agaagatgtc catttcggag ctcaccaacc
    51541 actggctcga tcgggtgttc ggcgaggggg aattccgctt tccccgggtc tggctccaaa
    51601 aggatgatct tgatcgcgcg gcccggttcg tggagggcct ccgggcggcg gggtgcggcc
    51661 gcgtcatcgc catcaatttc ggcgtgggtg gcaattcccg gaaacgcctc agcgaggcgt
    51721 tcgaaaggcg tttgatcgcg accctgctgg aggaaccccg gaccgtcgtt ctcctcgata
    51781 agggcttcgg ggaggaggaa cttggacgca cggcgcgcct gatggccgcc ttcgccgacc
    51841 ggccgctcct cgacatcccc ttcgcggccc tgcccgaccg ggcgatgacc ggcggcctcg
    51901 ccggcgtcga atgcgacatc ggcgagatcg cggcgctgat ctcccacagc gatgagttca
    51961 tcggctatga ttccgcttgt cagcacatcg ccgccgcggt cggggttccc acatatacgg
    52021 ttttcgcggg cagcaacaac acccgtttca tccggcgctg gcacgcctgc ggcccggtcc
    52081 ggagcgagat catccatgtg gacaccctga cgcatccgcc catgttcgac gacgaggacg
    52141 tcgtggcccg catcatcgac gccagaaggg tttgacggat tagcgggatg aaaggcagca
    52201 gatgaaagtc ctttttgtgt atccgaatgt cggctcccag ttggggttca attacggggt
    52261 ctcctacctg tcggccctgt tgaaggcggc cggccacgag gttgttttcc gccagatgtg
    52321 cgaagacctg gggccgctcc cgtccaagga cgaattcatc gcctggctga aggaaatcga
    52381 ccccgacgtc atcggctttt ccgtggtgac gaatcagtgg ccctacgcca gggaactcgc
    52441 cgggtggtgc cgggaggcga catccgcccc gctcgtctgc ggcggcatcc atgccacggt
    52501 tgcggcccgg gaggtcctgg cgaccggggc cttcgattac gtgttttcgg gagagtgcga
    52561 ggaggcgttt cccgagttca tggaccggct ggcccgccag gaggatgtct cggacctccg
    52621 gaacctgggc cacgtcgtca acggcgaaat ccggattaat ccgatccggc cgctgccgga
    52681 tctcgagtcc ctcccaccca aggactaccg gatcttcgac ttccagcgga tcatcgatgc
    52741 gaaaaacggc tgggtcggat tgatggcatc ccgcggttgc ccgttcaact gcacctactg
    52801 tttcaaccat gtgatggtga agaaataccg ggacgacctc gcgtgtagct ttcaggatct
    52861 ccactacatc cgtcaccacc gcgtggaaca gatgatcgac gagatcgtgt ttcttcagaa
    52921 gacgtacaag aacatcaaga tgttcctctt cgatgacgac ctgttcacct tcgaccgtga
    52981 ctatgtgatt cgattctgcc gggcctatcg gaaggtttcg tcgcttccct tcgtcgtcaa
    53041 cgcccacgtg ggcttcttcg acgaggagcg ggcccgggcc ctggcggagg cgaactgcaa
    53101 gatcgtcaag gtggggatcg agagcggcag cgagcacatc cgccgtcagg tgatgaaccg
    53161 gcgcaccagc aatcggagaa tcgtcgaggt gatccggctg atcgaccgat accggatgca
    53221 cagctcgtgt tttatcatgc tgggccttcc ctatgaaggg cgccgggaag tcatggaaac
    53281 cgtcaccctt ctgggggagg cgaggcccgg gaggttccga tggaccttct tcttcccgtt
    53341 tccgggcacg aaggcccatg acctgagcgt ccagggcggt tacgtgaact tcgaccggat
    53401 gggctcgctc atgaatttca ccgatgaatc ctgtctggat ttcgggcctg aacacaacct
    53461 gttcctgaag aaggtcggcc tcatcctgcc ctggttcgtc aatgcccacg cccgcctcga
    53521 ggtgtctccc ttctatcggg gccgggtgga cgcattgctc aagatggacc tggaaacgtt
    53581 cgaaatggcc gctccctcca tccgggagga agaccgtgaa atctcttccc gattccaggc
    53641 cgaaggccag acccactacg cgatcaagta caaccccttc atgggcgtga tttccgacta
    53701 ctttacccaa gagtaagccg ccgtcgttct cgtgcagtcg ggcggcgagt tcccggatct
    53761 tcgggaattc gtaggcgaag acggagcggt aggggtggat cttcccccga cggaagtcgt
    53821 cggtcgcggc gcaggacaag cccgcggctt cgatgattgg tggaacgggg cgggcccggt
    53881 caaacgatga aatcgggccg ctccccccag ttgccccgga gcgtcggata ggccagtgcc
    53941 aggcgaagcc ggttcatgaa ggttttccgg gcgatcatcc gggcccccag gctttgcaga
    54001 tggcgggtga agacctggca gtcgatcagc ttgaagtcgc gggctccaag ccgttcgacc
    54061 agtgtgacaa aggccgcttt cgaggcgttg ctgacctcgg tgaacatgga ttccccgaag
    54121 aagcagcgac cgagggagac cccgtagagg ccgccgacga tgttcccccc ctgacgggcc
    54181 tcgacggaat gcgcgtaccc cagctcgtga agggtcagat aggcctcgcg catctccggg
    54241 gtgatccagg tttcgtcccg tccggggctg ggccgtccgc aggcctcgat cacggcgggg
    54301 aaatcgcggt cgaaggtgat ggtgaaggcg tttttcctca ggagcgcccg cagactgccg
    54361 gagatgtgga gctcccgggg gaagaggaca aagcgggggt ggggcgacca ccagaggagt
    54421 ggatcctcct cgccgtacca gggaaagatc ccctgccggt aggcggccag gaggcgggcg
    54481 ggggagaggt cgccgccgac ggcgaggagc ccgctccggt cggccaggcg caccggtggg
    54541 aagaggatgt cctcggtcaa ttcgaatacg ggcatgggcg ctctatagca cacttcgggt
    54601 tgacaggcac gaaccgatcc gctatgggag cggtcgcgct gcatgcaaaa aaacgccgga
    54661 gacggaaggg gatgtgtcca tgaacctgtt tggaaagccg aagggaaaga agttgcacac
    54721 gcggaccatc gaggtgaata cctacgaata tggcgccgga cgtctggtcg tagagggtgc
    54781 cctgaaggat ctgcgctggc aggatcatta cctggccacc ggtgagaaga agccgccggg
    54841 ggttctgcac gggatgatca tccatttgct cgtgaacgtc cgcaacctgg agatcgagga
    54901 cgttcatgtg gagatgcccg acacgccccg cgaagaatgt ctggagaccc gcgggtgcat
    54961 cgcccgggtc aaggggatgc ggatcgcggg cggttttaca ctgaaggtca aggagatgct
    55021 gggcgggatt caggggtgca gccacctcct ggcccttctg accgccatgg cgccggccgc
    55081 cgttcagggc ttcgcggcgc acatcctcag ggacgatacg gagctgaaat cgacctgggc
    55141 gggactgtcc cggtttctgg aggacacctg ctgggtgtgg cggaaggacg gtccgctgct
    55201 gaagaaactc cagagtctgt aacggggtcg ccgagaggct cccggccgtc tgcgggccgg
    55261 aggatccgga ctcagaccga tcgcaccacc acgtcgtcgt cctcgatgtc ggcccgggcg
    55321 gctccaccct cgcggaggcg gccgaaaaga acctcgtcca cgaagaagtg cttgattttg
    55381 tcctggatga ggcggccgat ctcccgcgcg ccgaattccg gagaggaccc ccggcggacc
    55441 agccagtcgg tgcaggcgtc cgtcacctcc agcgtcacgc cttttccctt caactgaaga
    55501 cggaattcat cgatgcgttt ccgaacgatg tcgaggacca ccgtgtccgt gagcgcgttg
    55561 aaattgacga cgccgtccag gcgattccgg aattccgggg aaaaggtctt ctcgatggcg
    55621 gggaagaccg cgtcgcggtt cagcggccgt ccctcgaagc cgatgaccgg gcggccgatg
    55681 tcccgtgcgc cggcgttgga ggtcatgagg atcaccacgt tccggaagtc cgccttgcgg
    55741 cccgtgttgt ccgagagggt ggcgtagtcc atcacctgaa gcagggcgtt gaagatatcc
    55801 gtgtgggcct tctcgatctc gtccaggagg agaacggcgt ggggggtctt gcggatcgcc
    55861 tcggtcagga ggccgccctc ttcgtacccc acgtaaccgg ggggcgcgcc gacaagcttc
    55921 gccacggcgt gccgctcctg gtattcactc atatcgtagc ggtggagtgg cacgcccagg
    55981 acgtccgcca gctggcgggc cagttcggtc ttgccgacgc ccgtcggccc gacgaacagc
    56041 agcgaggcga cgggtttgtc cgcctcccgg aagccggccc gcgcccgttt cacggcctcc
    56101 acgaccgtct ccaccgccgc gtcctgaccg aaaatccggc gcttcagctc cgcctccagg
    56161 tccttcagct tctccacctc ggacgaggag acgctccgct ccgggatccg cgcgattttc
    56221 gctacgaccg cctcgaccgt ccggcggtcg atctcccgaa cgtcggaatc ctcaccggct
    56281 tccatgccga tcaccgcccc ggcctcgtcc agaacgtcga tcgccttgtc gggcaggcgg
    56341 cggtcgttga tgtagcgatg cgagagttcc gccgccgcct ggagggccgc gtccgtataa
    56401 acgacgccgt ggtaactttc ataccgggtc ttgacgccct taaggatctc caaggtttcg
    56461 gcggccgtgg gttccgggat ctcgatcttc tgaaaacggc gcgacagggc gccgtccttt
    56521 tcgaagtgtt tccggtattc gtcgtacgtc gtcgagccga tacagcggag gtttccggag
    56581 gcgagcgccg gtttcaggat gttggaggcg tccagcgagc cgccggagac ggcgccggcg
    56641 ccgacgatgg tgtggatctc gtcgatgaaa aggatgacgt tctccatctt ctgcaattcg
    56701 gcgatgaccc gcttcatccg ctcctcgaaa tcgccccggt agcgggtacc cgccagcagg
    56761 gcccccatgt cgagggcgaa gatcttcgcg tcccgaatct tcttcggaac ggcgccggcc
    56821 gcaacccgtt gggcgagtcc ctcggtcaga gcggtttttc cgacacccgg ttccccgacg
    56881 tgaacggggt tgttcttcag ccgccggcag aggacctgga gggtgcggtc gaggatctcc
    56941 tcccggccga tcagggggtc gaactcgccg gcgctggcct tggcggtcaa ttcggtcgtg
    57001 aaggcccgca ggatcttgcc cgcgtccggt tccttctgtt ccgccggttt cgcatcttcg
    57061 cgcgcaggcg gggtatccgg tcgcggaccg ctctcccctt ccggtggcag ggccgaaacg
    57121 ccgtgggaga tgtagttcag cagggacaga cgggaaatcc cttcttttct caaaaaatgg
    57181 gcggcgaaag attccttttc gtccaagagg gagacgagga cgtcccccag ttccaggata
    57241 tctttctgcg cgttggaggt gtgccagact gcccgttcca gaacactctg gaatccgagg
    57301 gactgaaacg cctggttgcc ctcgacgggg gtgtgcgtct cctggaggtg gcgcgccaga
    57361 agccgcttca ggtgttcgat gtttccgccg cagtggccga tgatatcccg gccgtcctcg
    57421 aagaacagac aggcgtagag cagatgctcc ggggtgatga attcatgatg gcgcacgttc
    57481 gcctcggcgt aagccgccat aatgatgcgg ttcagggttt cggagatctt catggcggtc
    57541 aggcttcctc cgcggagcac ttcaggggga attcgtgttt ttgggccagt tggtggactt
    57601 ggtcgatttt ggtcatggcg atgtcgtagg tgaagacgcc gcaggtgccc cgaccctttc
    57661 gatggacgtc cagcatgatg cgggtcgcat cggcggcggc tttgtggaaa atcgcgatca
    57721 gcacctccac gacgaattgc atggtcgtgt aatgatcgtt gtgaaggacg acccggtaca
    57781 tcttcggctc ctggtgcgcc acatcgtcat gaacgtcggg atgctgcgcc ggccgatcgg
    57841 gatggagcga ctggggcata tccacctcgc ctgggaaaac cggctgttcc gaccgtgccg
    57901 tccggccggg aaagggggtc ggcagggttc ggcctcaatg tggctgtaat ataggacacc
    57961 ttgccgggcg tgtcaatcca tgtgttagtg acggcaatgt ctcgggtctt gttgtgccag
    58021 aagcagaaga aggggtgtct ccccggtttg acgagaccga aagagaaaag atggcccatg
    58081 gaacaaaagg agatcatcga ttttaccatt gcccatggcg cgtcacaggc cgcttgggat
    58141 gccttcctgt ccgatcttga tgacaggcgg ggagcttatt tgttttatgg gcaggctgat
    58201 tcttgacagt ggaagaggtt catattatag gacttggaca tggttcgtca tcggatgaga
    58261 ggacgccttg gaaaaagcag acaatgtctg ggattttttt gacaagtttt actgcatttc
    58321 tctaaaggaa cgggaagatc gccgccggtc ggcgcgcgaa gaattttcaa aagtgggact
    58381 ggcggacaga gtcgagtttg tgctgggcga acgtctgccg tacgatctag agcagcaggt
    58441 ttatgaatca cacatggcat gcctgcgcaa agggctggaa tccggcgctc aaagcattgt
    58501 tatcttcgaa gacgacgtgg aattcgaccg ttttgacaca aaacatttaa taaattgcat
    58561 aaatttcttg agacagcatc ccgattggaa ggtcctgctt tttggcgctt taatccggtc
    58621 aagctgtaaa acagacaacc catgcgtgca aaaagtccgc tatcaaagcc tgacgcatgc
    58681 ctacgcctta aaccggcact atgcccagga attggcctat gagccatggg agggaatcgt
    58741 gaatgacacc ctctttcgtt ccctgacgga cggcatctat gcgctttatc ccatgtgcgc
    58801 gtttcaaaag gatttcaaat cggataatta caagtatccg aggctcgaaa aaatccggcg
    58861 tttgctgggc ggattaaacc ggattcaaaa gggctttgaa ttctactcac gccacaaatt
    58921 cggcatctac gcggttcaga ccgctgccat cctgcttgtg ctgttcgcat tcattgggcg
    58981 ctgaacagcg attccttccg cgcagctctg aatgtccaaa acatcgcatc ttttgacccg
    59041 tttaaggcca atatccctcc ggcgtggatc agaaacgaaa ttacattgac ttgcccctaa
    59101 aagagtaaaa accttcatcc cgttgatcaa gcattgaatt cttgacatcc cacggtggat
    59161 ggaactgaaa aaccaggttt tggaagcgga caggaggtgt gtgtgccgca cggagggatc
    59221 ggcgtcgccg atcccgggat gccgaggaag ccgttcgaaa gagtcaggcg ggcgggggtc
    59281 ctttccagct tcccgaggtg atgcgggaca tcccggcggt tgaaccggcg tacatgaaca
    59341 cccaggcggt aaaacagctg tcgtcaagcg tccggacgcg ggtgagggtc cggcggaatc
    59401 gggagccagg gttgccgtac cccttgaatc cctctacgat atcgaacatt tcgaaggctt
    59461 tacgcggatc cttgagtgga tagacctcgc ccgcgaccca cgcgtcctcc ccctcgggag
    59521 gggtcatgca ggggaagttt ttcccgctgt cgaaaagcag gcctggcgcc tgggcggtgc
    59581 cccggcaggt atggtcaccc cacgtttcga ggatgtgatg acggggcccc ccttccttga
    59641 gggtgccgta gacgaacagg tggtccacgg tccaaggcgg atcgaatccc agggaaacgg
    59701 cgttgaacat ccggtcgtcg aggccgtggg cgatcagggc ctcctgaacg gcggcgatgt
    59761 aatcggggtg aggcgggacg aaaatccctt cgatcatggc gggatcgact tggtaggtga
    59821 ccgcactgta ttcccggcca tcccccgtca gaacgaccac ggggaggcgc ttgtagactt
    59881 tcggcgcgtc ctcccttgcg tcgagggttg cccagccgtc cggcagcgtc tcgtaaagga
    59941 cccccggtat cgcctggccg gtccggtgcc ggaggttgga gaccccgccc ccccgccttt
    60001 cggagaggaa attgaagacc tgacgcatat cggggagcca ggcgttgccg acttttcttc
    60061 cgagcggata ggggaggcct ttccgatcgc accaggtctg cagatccgcc cgattcatgt
    60121 tggttccgta ggcaaaatac aggtcggttc ttcccatgat ctctctcctt gaccggaatc
    60181 acccaatgct agaattataa agatttcagg gttcttctgc aagctttttt aaccatgcgg
    60241 gcgggccgga ggggcgaatt cgcggttgat ttttggcgca tttgcgatta cggtcgactt
    60301 tgcggtcggt tcagcgggca cggcggacgg ctgagggtgc cgccaagcca acgaaatatt
    60361 ggaaggggtg gccccgggtg atacgggaaa gcgtctggaa gtctttttgc ggacggtgcc
    60421 gcctaccggg agcgcgccgg gactgtatcg ggttaaggtg aaaaaatgcg tgcagctaaa
    60481 actccaccgg ggcaaaccct tgcgggcagg aaggacgccg ggcgacggcg ggttttctgg
    60541 gcctttctgg ggctggtcct gacggtcctc cccgcctatg ccgtgactcc ggaggaggct
    60601 ctccggaagg acttcccgga gctgaagttc gaccggatcg gtcccagccc ggtcccgggc
    60661 atggccgagg tgatcgtcgg ccccgacatc ctgtactatc acccccggga gggggtcatc
    60721 atgacgggcg agatgatcag ccgggaacta aagaacctga cccgtcagcg gaaaggggag
    60781 ctccaggccg agcagatcaa aacccttccc ctcgatctgg ccgtccggat cggaacggga
    60841 tcccacaggg tgatcgaatt taccgacccg aattgtccct attgccgcca ggcttcgaaa
    60901 tacctgaaag acaggatgga tctgtcccgg tacatcttct tcttccccct ttcgcccaga
    60961 tccgaggaca agatccgcta tgtcctctgc gccccggacc ggggcaaggc ctacgaggat
    61021 gtgatggccg gccggaggga cggcgtcgaa ccggagattt gcaagaattc ccaagcgggc
    61081 gagattcaga aacggcatcg ggaccttggg cagcgtctcg gtctcgaggg gacgccgttt
    61141 ttcgtgatcg atggcgaggt ggtggcgggg gcggatctgc cccgcatgga gaaactgtta
    61201 gaggcaaaaa agccatgaag ctgcggcggg aagcgtacct cagacccctg accgtcctgc
    61261 tcgcctttgc cggggtgggg gtggccctgt attacctcac ctgcgatgac gcctgccggt
    61321 atctgtcggg ctcggtcttc cgcgtggatc taaaatactg gggcatcggc tatatgggag
    61381 gggttctggt cctggggctg ttccgcagga ccctgtggaa cagcctgctg ctgtccttcg
    61441 gtgttggcgg cgaggtcttt ctcgtcggat ttcaggtcgt ccaccgggtg ttttgcccct
    61501 actgcctgac gttcgccgcc atcgtgctcc tcctgtttgc cctgcacgtc cggccgggaa
    61561 agaaggcggc cctgctgctg ttcgccatgg ccgggctcct cacgtttctc ctggcgtttt
    61621 ccggatcggc aacgcccgtt tacgccgccg gaccggctca cgttccgacc ttcggaaaag
    61681 gccccgtcaa cgtccgcctg tacactgatt acgcctgcga accctgccag atgctcgaaa
    61741 cgcgagcggc gcccctgctc gacgcgctgg ttcgcaagga caagatccga ctgaccttca
    61801 ttgatacgcc catccacacg gaaacgaagc tctacgtcaa atactacctc tacgttctcc
    61861 gggagtcgca gacgctggcg tcggctttcc gtatccgcac gctcctcgtc gaggcggcgc
    61921 agaaaagggt ccgtgatccg acgaagctgg aggccttcct cgtcgcccga ggggtttccg
    61981 ttcgccccgt cgacgtgacg ccgaccctcg aggtcttcct gggctatctg agcaaggacc
    62041 ggattcagtt caccccgacc tgtgtcgttg aatcgcgcgc cggaaagaag acctatacgg
    62101 gcgtcaatga tattcttgcg gcgctgaagc gcatccgtta ggccccccgc gacgctcccg
    62161 tcctcgtctc cgaaccgggc tggagggatg cccaagcccc gcttcccttt cggtcatggg
    62221 ggtatcggac cttccgtcga ttccggtttc cgctcccgtc gcaaaatccc gggtgcaatc
    62281 cgttgcgttt tcggctataa gaggcgggac gggtcggaag atccgtcccg ccggtccggt
    62341 gcggagccgg gccaggacaa cgggagggcg aacgtgatga aggatgtgtt tcaggccgtc
    62401 caagtgacgg atcatgtgta ctgggtgggt gcgatcgact gggacatccg ggatttccac
    62461 ggctatacga cgccccgggg cagtacgtac aacgcatatt tgatcctggg cgatcagatc
    62521 gcgctgctgg atacggtgaa agcccccttc atgggcgaga tgatgtcccg gatcgcctcg
    62581 gtcgtcgatc cgcaaaagat ttccgtcctc atctccaacc actccgagtt ggaccattcg
    62641 ggcggcctgc ccgccgccgt tgaagcgatc cggccgggaa aggtgtatgc ctcagcggtc
    62701 ggtgtgcgca ccctccagga actctttcac ggcgcgctgg aggttctccc tctgaaggac
    62761 ggtgaccggc tcagcctcgg gaacctgaac ctgaccttcc tggagacccg gatgctccac
    62821 tggccggaca gcatgttttc ctacctcgag gaggaggccc tgctgttttc ccaggacgcc
    62881 ttcgggatgc acctggcctc cctggatcgg ttcgatgacg agatggatcc ggacgtcctg
    62941 aagcatgaag ccgcgaccta tttcgccaac atcctgctcc cctatgcgcc gctggtgacc
    63001 cagcttctgg cgcgggtcgg caacctcggc ctccggctcg ggatcgtcgc tccggatcac
    63061 ggacccatct ggcggaagga catcccggtt atcctcgacg cctatgggcg ctgggcgagg
    63121 caggagccga cgcggaaggc ggtcgtcgtc tatgccacca tgtggcacag cacggagcgc
    63181 atggcccggg cgttgagcga gggactgaag gcggagggag ccgaggtcag ggtcatggcc
    63241 atggatcatg tccatcgcag ccaggtggcc tacgaactgc tcgaagcggg tgccctgatc
    63301 tgcggctcgc cgaccctgaa caaccatctc ctgccccaga tggccgacgt gatgacctat
    63361 ctcaaggggt tgaagcccag aaacctcatg ggcgccgcct tcgggtccta cggctggagt
    63421 ggggagtccg tcagacagtt gaacgacatc ctggtggaga tgaaggtgga aaaggtcggg
    63481 gagggaatcc gggtcaagca ccggcctgac gcgggtgtct tggccgactg ttatgccctc
    63541 ggacgggaga tcggggagcg gttgcgcgcg aaggccgcgc ccgcggcata aatcacttca
    63601 agacaaggag cggacccatg aaaagttacg agtgcggtgt gtgcggctat gtttacgatc
    63661 cggccaaggg cgatccggac aacgggatcg cccccggcgt ccccttcgaa gacctgccgg
    63721 aaggttggac ctgtcccata tgcggtgcgg aaaaagatca gttttcaccc ctatagagga
    63781 agactcatgg caacgattag agatgcgctt tatcaggcct acacgggaga ggcgaaagcg
    63841 gcccttaggc tcaaggttta cgcggaaaag gcggcggagg agggctacct ccagatggcc
    63901 aagctcttcc gggtgatcgc ctattcggaa gagattcacg gggcgcgtgc gctccgggtg
    63961 ctcagggaga tcaagtccac ggaggagaac ctggccgaca gcttcgaatc cgaaacgcgg
    64021 gtggcggaag tcgcctacga acagttcatc aaacaggccg aggcggaggg aaacaagccg
    64081 gcgctcctcc atttcagcca gagccgggat gtcgaggaaa cccacgccaa gctgtacaag
    64141 gaagccatga cccatttcct cgaggagcgg gaaacgcact atcacgtctg caagatctgc
    64201 ggatatgtcg ccgacggcgt tcttcccgaa gaatgtcccg tctgcggtgc gaagcgcgac
    64261 cacttcgtct cctttgactg atccgcctcc ccgcttgaaa agcgggggat tctgtgttag
    64321 ctacagcgcc aaccaacgat gcaggagcgc tccatgaact acaagaatac gttgaacctc
    64381 cctcaaaccc cgtttcccat gaaggccaat ctgtcccaga aggagccgga catgctccgg
    64441 cgctgggaag agatgcggat ttacgacaag attcgcgagg cctcgcaggg gcgccccgtc
    64501 tatatcctcc atgacggtcc cccctacgcc aacggaaata tccatctggg cacggccatg
    64561 aacaaggtca tcaaggacat ggtcatcaag gccaagaaca tgtccggtct ggattgcctc
    64621 tacgttccgg gctgggactg ccacgggctt ccgatcgagc atcaggtgga caaggaactc
    64681 ggagagcgaa aggagggcct gtcccaggcg gacaagcgcc gcgcctgccg cgtgtacgcg
    64741 gagcggttcg tggacgttca gcggcagcag ttcaagcgac tgggcggatt cggggagtgg
    64801 gaaaatccat acctgacgat gcactacgaa tacgaggcga tcacggtggc ggagttctgc
    64861 aagctcttcc tctcggggga cgtctacaag ggcaagaagc ccgtctactg gtgcgccacc
    64921 tgcaagaccg ccctggcgga ggccgaggtc gaatacgccg accatacgac cccctcgatt
    64981 tacgtcaaat tcccgatgat ttccgacgtg gcggccgtcc ggcccaagct ggccggggag
    65041 acggtcagcg tggtcatctg gacgacgacc ccctggacga ttcccgccaa cctggccatc
    65101 gccttccata aggatttcga gtatgtcgcc ctaaagatcc gaggcgaggt cctcctcctg
    65161 gcgaaggagc tcatcgacta ctgcctggac gccttcgggt ttcgggggga ggcttatgag
    65221 atcctggacg agtttcccgg ggccgcggtg gaggggctca agtgccggca tcccctgatc
    65281 gaccgggaga gcctgctcat tctcgcgcct ttcgtgaccc tggaggcagg aacgggctgt
    65341 gtccacattg cccccggcca cggtcaggag gactacgaga tcggcgtggt ctacggcctc
    65401 gacaactatg ccccggtcga tgcggacggg aaatttacgc ctgacgtcaa ggatttcgcc
    65461 ggccagttcg tgtttgacgc caacgacgcc gtgaacgata agctacggga agccggcacg
    65521 cttctggggc gcgtcgacat cgaccattcc tacccccact gttggcgatg caaggagccg
    65581 atcatcttcc ggtccacgga gcagtggttc atttccatgg acaagaacgg cctgcgtaag
    65641 aaggccctgg aggccatcga ccgggtgcaa tggatcccgg cgtggggccg cgaccggatc
    65701 tacggcatgg tcgaaaaccg accggactgg tgcatctcgc gtcaacggct ctggggggtg
    65761 cccatcacca ttttctactg ccgccattgc cagcaggaac tcctgacgac cgagatcgcc
    65821 gaccggatcg tcgccctggt ccgagaacac ggggccgacg tctggttcga gcggacggcc
    65881 aaggaactga tgccggcggg cacggtctgt ccgtcctgcg gcggcgatgc gtttaccaag
    65941 gagacgaaca tcctcgacgt ctggttcgat tcgggcgtca gccatgccgc ggttctcgag
    66001 acccgccagc gacatcgctc gcccgcggac ctgtacctcg aggggagcga tcaacatcgg
    66061 gggtggttcc attcgtcgct gctcgaatcg gtcgggacgc gcggacaggc cccctacagg
    66121 agcgttttga cccacggatt cgtcgtcgac ggcgacggca agaagatgtc caagtccgtg
    66181 ggaaacgtcg tcgatcccca ggagatcatc gatcggtacg gggcggaaat cctccgcctg
    66241 tgggtggcgg ccgaggatta tacggccgac atccgcatct ccgaggagat tctgaagcgg
    66301 ctcgtcgagg cctatcggcg catccgcaat acgggccggt tcatcctggg caatctgtac
    66361 gatttcaacc cgaagcggga tcgggtggcg cctaccgaga tgatggacat cgaccggtgg
    66421 gccctcctca ggctcaagga gatcatccga cgggtccggg acgcctacga acgctaccag
    66481 ttccatgtcg ccttctatac cctgtacaat ttctgcaccg tggacctgag cgccctgtat
    66541 ctggacgtgc tcaaggaccg gctgtacacg tcgcctgcgg cttcactcga gcgccgttcc
    66601 gcgcagacgg cgatgatgac gattctcgat gccatgacgc gcctactcgc gccgatcctc
    66661 acgttcaccg cggaggaact ctggggcgcc ctgccgccgt tcgacgggaa ggcatccagc
    66721 gtccacctga cccagtttcc ggaaccgcaa acggaccccg acgatgcggc cctgggggag
    66781 cgatggaaga cgctgatcgc cctcaagggg gagatcgcca aggccgtcga gatcgcacgg
    66841 caaaacaagg tcgtcggcca ctccctcgat gcggcggtgg cgctcacgct gccggagatg
    66901 ctgcgcgcgt ttgtggaagg tgcgcgggag gagctcaaga ccctctcgat cgtttcccgg
    66961 cttgtcgtgg tcgagcccgg agcgatggat gccgcccctt atgccagcga agagttcccc
    67021 gggctgatga tcggcgtctc gaaggcgccg ggcgagaaat gcaaccgctg ctggatgttc
    67081 agcgagaccg tcggttcacg gcctgcgcat ccgacccttt gcgagcgctg tgcccgcagt
    67141 ctcgaaccct gaaggagtgg accgtgccac gcaagtatgt cgtgcttgtc atcacgaccc
    67201 tcgtcgtggt gtttctggat cagggaacca aggcctatat cgcgggaacc cttcccctgc
    67261 atcaatcctg ggaagtcctg cccggtctgt tcaacatcac ccatatccga aatcccggtg
    67321 ccgcgttcgg gtttctctca ggcgcatccc cccggttccg gatcctgttc ttcattgccg
    67381 tcacccttct cgctctgggg ctcatcctcc attacatcta ccgggcgacg gcgggggaag
    67441 gattccaggc ggcctgcctt tccctgattt tcgccggcgc cgccggcaac ttgatcgacc
    67501 gcgttcgttt cggcgaggtc gtcgattttc tggacgtaca ctggggcgtc catcactggc
    67561 cggccttcaa cgtggccgat gccgccatct ccgtcggggc gatcctgctc atcctggcgc
    67621 tgatccggga aggccggtca aagacctgag aaagggaaaa gacattcaaa agtcacgtga
    67681 gcgtgaccac cgtcggttta cgtgagcgta gacccaggaa cggacgcctt gaaccgtttg
    67741 cggctcgtct tcattctcct cattctcggt ggaggtctgg ggtttttatt catcccggcc
    67801 gcggttcccc ccgattcagt attcctcccg atcatgcctc catcccgggc ggacgcttgg
    67861 aacgccccgg ctgaggatca ttgcggcctc cgggtcgtcc gcgcttatcc gcacgatccg
    67921 gaggccttta cccagggcct gttctatgag ggcgggaacc tttacgaagg caccggcctt
    67981 tacgggaaat catcgctgcg ccggctcgac ctcgtctccg gacggatcgt tcgtatccgc
    68041 cgtctttcgc cggcgttctt cggcgagggc gtcacctcgt gccggaatcg gatcattcaa
    68101 ctgacctggc gcgagcgggt ggggttcgtc tacgatcttc aaaccttccg cctgctgggc
    68161 cgttttcccc gggagggcga aggctggggc atcacctgcg acggtcggcg tttgatcgtc
    68221 agcgacggca ccgaccgcct gcagcttctc gatcccgata cgtatcgtcc cgtcggatcg
    68281 ctttccgtaa gggatcggga gggacggtcc gtcgcgcggc taaacgaact cgaattcgtg
    68341 gaaggagaga tcttcgccaa tatctggcag agcgaccggg tcgcccgcat ttgtccgaaa
    68401 accggtcggg ttgcgggatg gattcatctg ccggaacttg agcggggccc ggccggtgaa
    68461 gagccgaacg ggatcgccta cgacgcgagg gggaagcgcc tgttcatcac gggtaaactc
    68521 tggccgcacg tctatgaagt gatcctcacc cctctggtgg ggccgcctcg aacgaaggaa
    68581 acgggtggcg cggctccctg agcagggctg acagcagggg atcgcgtccg tagacgtcgg
    68641 gtcggaaatg gacgttcccg tccgcctcag cccaggacgt gacatagacg agatagacga
    68701 gaaccggctg gggcagattg atagtggtgg ggcgtttttt catgagcccc tcccggatca
    68761 acttctccgt cgccggggtg tcgccgaaca gatgccgcac cagcgcgagg ggtttttcca
    68821 ctcggacgca gccgtggctg aaggtgcgga catgccgccg gaaatgccgc ttggaagggg
    68881 tgtcgtggag gtagatgtcc ggcgtgttcg tgaggacgaa cttgatttgt cccagggcgt
    68941 ttcccgctcc cggatcctgt cgcaatgtgt aggggaagtg gtgcacggag aggctttccc
    69001 acgcgatctc aaacgggtcc aaacccgttt tatctctcgc gcttctgacc acctgaagac
    69061 gttgtctttt caggtaatcc ggatcggctt tgattttggg cagcatttcc cgggttgcga
    69121 tcgatctggg caccacccag aaggggtgga acacgatctg ggacaggagg gaattgaact
    69181 cgggggtttg ccactccgga cgggtctggc ccacgacaac cctgagttcc aagaccggga
    69241 agccatcccg cacggcgcgc agccggaaat ccgggatatt gacgaggaga taatcgtccc
    69301 ccagctgtcg gcacagccat cgccaccgtt ccagattcag ttcgatcagg gccagccggt
    69361 cggctgccgg gacgctcatg gcctgacggg tgtcggcgtc gacggcgccg gatgctttca
    69421 ggccgtggcg ctgctgaaac ctcttgacgt cctcttccag gcgggtatcg aagtcttccg
    69481 gtgttgcggt ggattcctcc cccaggccct cttccgcggc caggcgttgg cggatcaggg
    69541 acacctcggg gccctgggtt ccccggcgaa gcgtcggccc gggaggaatg accggccagc
    69601 ctccccgggc gacgattccc cgatagcggg acagcgcggc ccgtaggcgc agaaaatagc
    69661 cgtgccgagg agagaagtcg aggagggcct caccgacgcg tccctcggcg agtgccttct
    69721 ccagggtgtc ggccagatcc gtatccgggc agaggtttgc gccgggcgcg tcgccgcggg
    69781 cgtacccggt ccggccattc gacagatgac gaccgcagtg caggaaggtg tccgtcatga
    69841 gccattcgaa ccgcgaccgg ttctcagggg cctgcccgat tggagcggat gcggatggcg
    69901 aaaggcgggc ggagagcgtc tccaagcgtt gcaggtgata gtctgacgga aagagtcctt
    69961 ccgtgtccac cctgcgcagc gtgtcgagaa aatccatggc cgcggggctc accccggcgg
    70021 catccgtcca ggccggccgc caggcgcgat tttcgtaaaa ttgccggacg agggcggatt
    70081 catagagggc ctcctcttcc ggcccttgga atgtccggtc gatcgtggaa aacggggtcg
    70141 gggagagggg gggcgaatcg gacggcgcct gggcctgcag ggggccggtg gccccggtaa
    70201 agacgacgaa gagggcaacg ccgagaaaga tccttgtacg cgagcgcccc atgaattcct
    70261 ccgttgccgg gtgttcccac cggtgcccca ccggacggcc ggtcgcggcg ccgatggaca
    70321 ggcttgcgcg caccgttgcc gtggacgcta ccacgcggcc ggggggattt caaggcgatt
    70381 ctacttcatc aggccgggca gatagagggc gatctggggg aaggccagga tgagaagggc
    70441 cgtgacgacg agggcggcca gcagatgggt cgttcccttg aagatgacgt ggagcggcac
    70501 gtcgcgggct acgccggcca cgacatacac gttgatgccg accggcgggg tgatgacgcc
    70561 catctccgcg gccaggacga tgatcacgcc gaaccaaacg gggtcgaaac ccagggcggt
    70621 cgcgacgggg aagacgaccg ggatcgtcag catgatcatg gccagggaat ccatgagaca
    70681 tccgaggagg atgtagacga cgaggaccag cgccgcgatc agcatgcggt gcagatggag
    70741 gccggagacc cattccccga tgccggaggg aatgccggtg accgccagga aatggccgaa
    70801 gaccgtcgcc ccggcgacga tgacgaagat catgcaggag atccgggtgg tgtcgttcat
    70861 ggcgcgcaga aacccccgcc aggagaggtt gcggccgatc acggcgatca ggagggttcc
    70921 gaaggccccg atggcggccg cctccgttgg ggtgaaaatg ccggcgaaga gtccgcccat
    70981 caccaggatg aacaggagga gcgtttcgat gaggccggcc agagagcgga gtttctccgc
    71041 ggcggttgtc cgtggccccc gcggcccgat ctgcggttgc aggcgggtcc agatcaggat
    71101 cgtcgcgatg aaaaggccgg tcagcagaag gcccggcaga acgccggcca tgaagagttt
    71161 cccgatggac tgttccgtca tgatcccgta gacgatgaaa atcgtgctgg ggggaatgag
    71221 gatcccgagg cttcccccgg ccgcgacgac cccggtggcc agggccggat catagcggta
    71281 cttcttcatc tcgggcaggg cgatcgaagc catcgtcgcc gccgtggcgc tggtggaccc
    71341 gcagatggcg gagaaggccg cgcaggcgcc gatggtggcc atggcgaggc cgccggggag
    71401 gtgccccacg aacttgtggg cggcgttgaa gagacggccg ctgatcccgg cgtggaaggc
    71461 gatctgtccc atgagcacga agagcggaat gaccgtcagg ttgtaggcac tgaaggtggc
    71521 gaaaaaatcc cgggcggcga ggttgaacgc ggccggccag gagacgagct gtccgaagcc
    71581 caggatgccg acgacggcca tgacgaagcc caccggcatt cggaggaaca tgaggacaag
    71641 gaggatgagg aaaccgacga tgcccacggt gatcggcgtc atgaccgcac cgccttgtcg
    71701 aatctcgcca gaaattcgga gaacaggacc agggccagca ggccgcaacc cagggcgatg
    71761 cccagggcga agggatagac gggcatgcca agggtcgacg acacctcccc gctttgcttg
    71821 agctcaaggg cataccgaag gctctgccag ccgatcagac cgaaaagaac gcctccgatc
    71881 gcgtcattgg cgaggccgat gaccgtctgg gcgcggcggg ggagcttctc caccaggatg
    71941 tccacggcga tgtttccctt ctccatggcc gtgcgcgcca gggcgaagga aacgacgagg
    72001 gtgcccagaa aaccgacgag ttcataagcg cctgggatgg gacgtccgaa gaagcgcagg
    72061 acgatgtcgg ccgtggacaa cagcatcatg gtgacgacgg cgccgctggc catgtggctg
    72121 aagaggtgac ttgcctggcg gatgatcctg tccggtcgat ccattgatct tttttcccag
    72181 ctccgaggtt gaggtgctcc cggcctcctt cacagcgcgg cggccgggag cgtttttcat
    72241 gatgatcgtt cttgacggtc ctatttcacg cccgtcgact gtttgtactg gcgataactc
    72301 gagaactttt tcttgccgaa ggccttgata gacgcctgga gatcccgcac cgccttctcc
    72361 gggttcaccc cctgcgtccg gttggcgtcg atgtactcgc tcacgaccgg tttaacgcct
    72421 cttacccagc gccgggactc ggcctgcgag agggggatga tccggttgcc caggctgagg
    72481 gtgtaccggc gtccctcggc gtcgaggtta tcccaggcgc gggcgtggac atcgatccat
    72541 tcctcgctga cctgttccag gaccttttgg atgtccgggg gcagagactg ccacttcttc
    72601 aggttcatcg ccacgaacat cgccgtcgtg taaccgatgg cggtcgattc cgtggtgctc
    72661 ttgaccacct cggcctgttt ccatcccttg agggtttcga tcggggcgaa cgtcccgtgc
    72721 acgatgccct tctggagggc ctcgtacgtt ccgccctggg gcatggccac gggaacggcc
    72781 cccagggctg tgacgacctt ggcgctcaat ccggtggagc ggattttctt ccccttcagt
    72841 tcctccagcg tcctgatggg cttcacggcg tgaagtagac cgggaccgtg agcgtggatg
    72901 taaaggagtt tgacgccgct gagctcacgg ggctgccagc gtcgatagaa agcgttggcg
    72961 acgaacgtgg cagcctgtcc actgggatag cccaggggga ggtccagagc ctccatgacg
    73021 gggaacctgc cacgcgtgta ggcgaaacag gacatgccca ggtccgagat cccgttcagg
    73081 acgccgtcga agcactgatc ggcctgggtc agggtgccgc cgtagaatag gttgatcttg
    73141 acgcgtccct gggtgcgttt ttcgatttcc ttggcccagg catcggccgc cttggcctgc
    73201 ccgtgtgtgg acggaaagaa gatgctgtag gtcagggtga tctcggcggc ccgacagtcg
    73261 atggccgtga cgttcaccat ccacaggagg accgcggaga caatcagcag caacgtgcaa
    73321 cattttcttt tcatggcgag gctcctttga cgaagggttt gggtttcggg aaaattatgc
    73381 aaaaaccgtc aaccgggaca actcgggtaa gccgcccggt tcaaccagaa ctgtttaaac
    73441 acgaagtcgg ggaatgggga tcggatcgcc tgctggaccg cacggaaggc ggacagcagg
    73501 catcaatacg tatagattgg ccgacagatc aaggcgcttg gtgcatggtt gggggtacgt
    73561 ttcatccggg atgaagtcct ctccgctacg gagtccattc ggcacgatgt cggcctttat
    73621 gaaatttcac cgggactgtc aagcgattta tttctggttt ccatctttga gcttttccag
    73681 ctcttcccgg tataattccg cccgggcttt ggcgagttcg atttcttccc tggcttggtc
    73741 gagcttctcc tcgtgacggc gcttctcctc gcggatcttg tcctggaggt cgtcaaatcc
    73801 gacgtagacg gccagaatcc ctcccaggag aagggcgatg gggagaccgc ccttgagcag
    73861 gatgaaaaac gcctcatgcc atccgatcag accgaccagc ccgagaacga ccgcgacggc
    73921 gccgcccaac atcaaagaat tcaatggcat tttcctcctc cttccatggc gggtccattg
    73981 cgtggcggat cgccggccac cggcatgaca cgggtgcttc ccgatccgcg cgacgttcga
    74041 actgaacccc gcatacagcc cctctgccgt tgccgactat cataaaatgg gttggtctgc
    74101 aagatgaatt cattccggct gtcgccgcgg tgatctatgt tttccaccgg tcggcgtcgt
    74161 gcgatcggga aaataatcct tgataaattt caccccttcc attagcatcg ttcgcactgc
    74221 cgccgggcgg gaggcatcgg ccggggccgc tgaaaacctt tccggcgccg gcttacccgt
    74281 aagatagatt ttcaaggggc gccgaaactc atcgtcggga taggaacgtg tgcaatgaca
    74341 gagccggatt ttgacaaggg cgacggcctg ataccggtca tcgttcagga aagcgaaacg
    74401 ggggaagtac tcatgctggc ctatatgaat gccgaggcct ggcgggaaac cctccgaacg
    74461 gggaaggcca cctactggag ccgctcccgc aagaccctct gggtgaaagg ggagtcttcc
    74521 ggtcacgttc agtgggtgaa ggaaattcgg atcgactgcg accgggacac cctgcttctc
    74581 agggtccgcc aggtcggaga cgcggcctgc catacgggat accggtcctg tttctaccgg
    74641 aaactctctc cgggttcgga agcggtcgtg ggagagcggg ttttcgaccc tgaaacggtg
    74701 tacaacaaag gctgaaggcg atagagcgca tcgggcgccg cccggttgat ggaggaaccg
    74761 gttcggcgtc gtccgacccg gcccgggcag agagaatctg gaaagggaaa aacgcatggc
    74821 ccccaagaag aaaacgcagg ccgcgccggt gtgcggaacc ccgggacgca ggctcaaaat
    74881 cggaattccc aaaggcagcc tcgaaaacag cacgatcgac ctgttcaagc gggccggttg
    74941 gcggatcacc tatgacagcc ggagctattt ccccgacatc gacgacgaag agctcttcag
    75001 cacccttgtg cgggcgcagg agatgtcccg ttacgtcgag gacggaacgc tggacctggg
    75061 actgacgggc cgggactgga tcttggagaa cgaatcccgc gtcgtcgttg tccaggacct
    75121 cgtttactcc aaggcctcca tgcgtcccgc gcgctgggtg ctcgtcgtcc gggaggattc
    75181 gccgatccgg tcgctggccg atctggaggg aaagcgcatc tcgacggagc tggtcaactt
    75241 cacgaagaag tatttcagga aacaaaagat caacgtgcag gtggagtttt cctggggggc
    75301 gacggaagcc aaagtggtcg agggattggt ggacgccatc gtggaggtga cggaaaccgg
    75361 cagcaccatc cgggccaaca agctccggat cgttcaagaa ctcctgaaaa ccaataccca
    75421 gttgatcgcg aatgccgagg cctggaagga cccctggaaa aagcagaaga tcgagcagat
    75481 ccggaccctc ctcatgggat cgctgcaggc catgaacaag gtagggatca agctgaacgt
    75541 cgcccgtaaa aacctggggc gggtcatgct cctgttgcct tccctcaagg cgccgaccgt
    75601 ctccagcctc tattccgaag attggtttgc cgtggagacg atcgtcgata tggcgattgt
    75661 gcgggagctc atcccgctcc tgaaggaggc gggagcggag ggcatcatcg aatatcccct
    75721 gtataaagtc atttgaggtg gccatgaaaa gaaaggcccg gatacacaga aagacgagcg
    75781 agacggacat cgccatcacc atcgtgatcg agggaacggg tgtggcccga gtgaatacga
    75841 cgattccctt cctcgaccat atgctggccc tgttcgccaa gcacggccgc ttcgacctgg
    75901 acgttcaggc ctcgggcgat acggaggtcg acgaccatca cctggtggag gatgtgggca
    75961 tctgtctggg gaacgcgctg aaggaggcct tgggggggaa atggggcatc aaccgttacg
    76021 gaaacgccct gacgccgatg gatgaaaccc tctgcagcgt ggccctcgat ctgtccgggc
    76081 ggccctatct ggtgtaccat gtcaagttcg gaaaccggaa aataaagcag ttcgatgcgg
    76141 cactcctgca tgaattcttc aaggccctga ccgaccggag tggaatgaca ttgcatatca
    76201 atcttgtcta tggtaaaaat acccaccata tcgcggaggg aaccttcaag gccttcgccc
    76261 gggccctgat ggaggccgtc gcgattccgg aaggtcggac ggaccttcct tcgaccaaag
    76321 gactcctgta aggcgcttcc cgccgggggg agacgccgcc gggccggcag ccggcatcgt
    76381 tcatgaagac ctgcgtgaca ccgcttggaa aggcctgcct tcttatggcc atcgtcgttg
    76441 ccctgtgggg tacggcggcc tgcgcttccc gacccggggt tgccgccggc gttccgctca
    76501 aggagacggc tgtcctgaag cggattgccg ttctgccctt tgcggaaatc gcccccgatg
    76561 aaacctccgg acgctcggga cggtgtcccg tgtgcggtgc cctgttcagc ggggaagcga
    76621 tgccggaagg ggctgcaggc gtcgttgaga gcgcttttct ccggcggatc gaggcggggg
    76681 gcaaattcga tcttgttccg tccgaccgga cccaggccgt gtaccggcgc cttgaggcgg
    76741 cggccctgaa acagacgacc ctgcagatcc tgcgggcggt gggacgggat ctggcggtgg
    76801 agggcattgt ggccggctac gtctaccggc ttcgggagcg gaaagggtat ccctatgccg
    76861 cggaacgtcc ggcatcggta gcattcgaga tccacctgat ccgcgtcagc gacggcgctt
    76921 ccgtctggcg gggtatttac gaccacaccc aggcctccct gatggagaat atcctccatg
    76981 ccgcttcttt ctaccgggag aaggggcggt gggtatcggc ggcggaactg acggacgagg
    77041 ggatggaggg ggttctccaa aactttcccg gactgaatta ggcggacgct tcgttcccgt
    77101 tatcgtcttc ggaatttctg ttggcaggaa attccgaatt tttttcatgg agcacgatcg
    77161 tgatcctcat cccggccatt gatctcaaag acggccagtg cgtgcgtctc cgccagggag
    77221 acttctcgcg cgtaaccgta tatggggaag atcccgcggc catggcgacc cactggtgtc
    77281 accagggcgc gacccggatc cacgttgtcg atctggatgg ttcccgtgcc ggagaacccc
    77341 ggaatcgcag ggccgtgcag gacatcgtca gaacggtacc catccccgtg gaagtcgggg
    77401 gaggaatccg gacgctcgac acgatcgccg actatctcga tctgggggtc cggtgggtga
    77461 ttctcgggac ggcggcgatg aagaacgccg ccctcttgga ggaggcgtgc cggcgctggc
    77521 cggggcgcat cattctcggt ctggacgccg tcgacggacg cgtggccgtc gaggcctgga
    77581 cggagagcac ggcggaaacg gcgatcgaac tggcccggcg ctacgagggc cttgcgctcg
    77641 cggccgtcgt ttataccgac atccatcggg acgggatgga agtgggggtt aatgtcccgg
    77701 cgacgaaact cctggccgaa tcgctccgcc tgcccgtgat cgcttcgggc ggtgtggcgg
    77761 gaatcgcgga tatcgagaaa ctgctggaaa tcgaaaatgc cggcgtcatg ggcgtgatta
    77821 tcggaaaggc cctgtacacc ggcgccatat ccctgccgga ggcactccgt ttgcgggagg
    77881 gaagtacgcc ggcggcatcg cacggaggtt gatgtcatgg aggattgcgt tttctgcaaa
    77941 atcatcagcg ggaccattcc cgccggcaag gtttacgagg acgagcatgt gctggccttc
    78001 gacgacatcc atcccatgtc cccgatccac gttgtcctga tccccaaacg gcacgtcccg
    78061 accctgctcg atctggcgcc gtcggaggac gatctgcccc gtcatctgat ccagggcgtg
    78121 caggcggtgg ccaggatcaa gggcctggat cggaacggat tccggatggt gatcaattgc
    78181 atggaagacg gggggcaggt cgtttttcac ctccactgcc acatcctggg ggggcagcgc
    78241 ctgaaggacg atctggcctg aagggctcgc cgctattgac aaccgatcga ggtgtactaa
    78301 cttaacccta tgccggaagg atgatccctc atgaattttc gtgaaaagat gaccgaggac
    78361 atggttcagg ccgcaaagca ccgggaaaag atacggctct ctaccctgcg gctgatcaag
    78421 acggccctgc acaaccggga aatcgatctc aagcgtgaaa tgaatgaggc cgaatttctc
    78481 cagatgctcg cctccatggt caagcagcgc agggactcca tcgagcagtt cggcaaggga
    78541 gggcgggccg atctggttgc ccaagaggaa gaggaactcc ggatcatcca ggagttcatg
    78601 ccccgcccct tgtcggacga agagattgaa cgggagatcg acctggccat cgacgaagcc
    78661 ggagcggccg gtccgaagga tctgggaaag gtcatgaaaa tcctcatgcc gaaactcgcc
    78721 gggaaagccg atggcaagac cgtgggagag aaggtcaagg caaaactgac cggggcgtag
    78781 gagaggcgga cggatcttga agggatcgat acccgcggac aagatatccg agatccggga
    78841 tcgcacggac ctcgtggctc ttgtgtcgga atatgtcacg ttgaagaagg cgggacgcaa
    78901 ttacctgggg ctgtgcccgt tccatcagga aaagacgccg tccttcaccg tcagcccgga
    78961 aaagcagatg ttttactgtt tcggctgcgg ggaggggggc aacggcttcg acttcctgat
    79021 gaaggcgagc cacatgacgt ttccggaggc ggtgcgccat cttgcggcaa aaacgggcgt
    79081 cgtcattccc gaaccggtcc gatcggccaa ggagaaagcg caggacggcc ttagagagca
    79141 ggcggtccgg gtcaacggcc tggccgcaac tcatttcgca cgaaacctac acggggccgc
    79201 ggggcgtgtc gcccgggcct atctcgaaaa caggggaatc agcgaggaga cgatccaggt
    79261 gtttcgtctg ggctatgcgc ccgacgcttg gcgttcgctg cgggattacc tcgcccggtc
    79321 gggcgcgccc gtggacctgg cggagaaact cggcctgatc atcccgggga aggacggtgg
    79381 cttctacgac cgtttccgtg cccgtctgat ctttcctatc gaggacgccg ccgggcgggt
    79441 ggttgccttc ggcggcagga tcatcggcga gggggagccc aagtacctca attctccgga
    79501 atcgatcctt ttcagcaaag gtcggcagct ctacgggctc aatcgggcgc gggaagagat
    79561 gcgtcgacag gattgcgtga tcctggtcga gggctatttc gatctgattg cgctttatca
    79621 ggccgggatt cggaatgggg tcgccaccct gggtacggcg ttgacccgtg atcacgtgga
    79681 ccttctcagg cggtataccc ggaatgtcgc ggccgtgttc gacccggacg aggctggacg
    79741 aaaggcgctg gcccggagcc ttgaagtctt tctggctggc gggatgcagg cccgggcggt
    79801 gattcttccc ggcggccggg acccggatga gtttgtgcgg gcgtcgggtc cggaggcctt
    79861 tctcgaggtc atcgcacacg ccccgtcggc cgtcgattat tatatcgatc ggatgatcgg
    79921 gtcacagcgc tctttcgagg agaagcaggc ggcgattcag gcggccgttc cgttcatggc
    79981 ccggatcgcg gatccggtca gccgcgagct gttcatccgg cgcgtggcgg agcgggtagg
    80041 gatcggcgag ggcctgctga aaaaggaagt ttcgcgtggg gtcccgtctt cccgggcgga
    80101 ggttcccgtc tcggcggcaa gtaggaaacc ggcccccctc gaccccctgg aatggagcct
    80161 gttgcaactg atgctcgaac acccggagac gattccggaa atttcccgaa gcggcattct
    80221 ggactgtttc ctgcatgagg atttgaaacg cttcggcgcg tatctcgtga agcaggggtg
    80281 tgaagggcac gggcagacgc cctccattca ggaccttctg gccgcatggc ccgaagaccc
    80341 gatccgggag cgggtgcaga gactctccgt gaccgcgagc ccctacgatc ccggcatggt
    80401 ggacaagatt cgggtcgata ccatccaccg gatccggcgg cggtggttcc gggggaagca
    80461 tcggctgttg gccgaagccc tccgcgacgc ccaggacaga ggggatcagg cgggcagcga
    80521 ggaactcctg cgccggaagg aaagcctcct tagggaggag cagggcgtcg atatcaatcg
    80581 cagtaaagga tgaggggaga tcaccatggc gaaagagatc caatcggatg aattcaaaaa
    80641 actgatgacc ttgggcgagg agaaggggtt cttgacctat gacgatgtca atgaccttct
    80701 gccatcggat gtgatctcat cggaccagat cgacgatatc atcattctgt tcggagagaa
    80761 gaacatcgat atcatcgata ccgacaaggg ggataagatc gtcgttaaga aaagcccggg
    80821 cgaactcgtt gaacacaagg aggaagagga tgttctaggc gtgatttcgg ccggaaagac
    80881 cggggacccg gtcaagatgt acctgcgcga aatgggactc gtttccctgc tcagccgcga
    80941 aggggaggtc gagatcgcca agaagatcga ggaaggcaac aaggagatca tggacgccat
    81001 tttcgggatg gcggtttccg tgaaggatgt gatcgatatc ggtgccaaga tccgggcgga
    81061 ggaactgcgg atcaagaacg tagtggagaa cctcgaagat gaagacggat tcgtggaaga
    81121 ggacctgcat cgtgaacggg tgctcaagct gatcgagaag gtcgcgatgc tggaccggaa
    81181 aaatgacgtc atgcgccaga aacttcgggc gaaggggctg gccgaaaagg aaaaggcgct
    81241 gtttcacgaa gagctggcca agaatacgaa ggggatcgtc aagtattgtc gaaaggtgcg
    81301 tttcagcaag cgtcagatcg atcggatggt gttccgcttg aagagctacc tcctggagat
    81361 cgaagaggcg gagcgcgaaa ttcagaaggc caaggacctc acgggcctca acatcaagga
    81421 gatggagcgt ctctgggtgc gcctgaggaa atccccccaa gaggccaaat tggccgcccg
    81481 cgaacggcgc gtctccctcg aaagactgaa ggcgacggag ccccgcgtcc ttgaggcgca
    81541 gcggcagatc cgtaggattt tactggaaac gggccttccc ctggccgcac tgaagagggc
    81601 ggtgctgtcg atcgagaagg gggaatggaa agccgaaatc gccaagcgga agctggtcga
    81661 agcgaatctg cgtctcgtgg tcagcatcgc caaaaagtac acgaaccgcg ggctccagtt
    81721 tctggatctg attcaggagg ggaacatcgg ccttatgaag gcggtggaca agttcgaata
    81781 tcagcgcggg tacaaatttt caacctatgc gacctggtgg atccggcagg cgattacccg
    81841 cgccatcgcc gatcaggccc ggaccatccg aattcccgtc catatgatcg agaccatcaa
    81901 caaactgatc cgcacgtcgc gctatctcgt ccaggaactg ggtcgcgaac ccacgccgga
    81961 agagattgcc aacaagatgg agtacccgct ggagaaggtc cgcaaagtcc tgaaaatcgc
    82021 caaggagccg atttccctcg aaaccccgat cggtgaggag gaggacagcc atctgggcga
    82081 ttttatcgag gacaaaaagg tcatgtcccc ctcggaggcg acgatcaaca tggacctcgc
    82141 ggaacagacg cgcaagatcc tctccacgct gacgccgcgt gaagagaagg tcctgaggat
    82201 gcgcttcggc atcagcgaac gcttcgaaca ggccttcgag gaggaccggg atttgttcga
    82261 ggtgaagcag attgaaatca agccgatgga cgccggtcag ggacgaaagc tgagaaaccc
    82321 gtcgcagaga cgattggccg ccccgttgcg ggcgaaataa atgattgaca aggcggccgg
    82381 gaggggttag aaagccactc cgtccggcaa agcggcagga cgatcgagcg ggcccatagc
    82441 tcaactggta gagccaccgg ctcataaccg gtcggtccct ggttcgaacc caggtgggcc
    82501 caccatcatc gaaggaacgt tcagcgggat gctatcaagc acgacatatc gggatggccg
    82561 ggattgcagc atgccttgcc catcagagca ggttcagggg gaggagaccc cgtgatcgcg
    82621 catgcaccgc caccggccgg tgcttttttt ttccattccc gtgggaaggg agcaggtgat
    82681 tgaaggatac gatctcgtta ttggttgcgc tacagaccct ggattcggaa gcggttcagg
    82741 tcgcggcccg gaaacagtcg ctgcctgaga agattcgtct cctggaggaa acacggcaga
    82801 aacagcaggg cgccctggat gaagcggagg agaagctcag ggggctgacc cagcggcacc
    82861 gggaccagga ggagcagttg aagcgcggcg tggagggact gaaaaaggcg aagcagcgct
    82921 tgctcgacgt caagaccaac aaggagtatc aggccatcct gaaggagatg gaggtcattg
    82981 aaacgcttca ggggaaagtg gaggacgaga tcattgcgct gctcgagcag gtggaccaca
    83041 tgaaggcggc cgtcaaacag cacgaaatag acctgtcggc ctttctagcc gtctttgccc
    83101 aagagaaaca ggaacttgag gggcaggtcc aggcgctcga cgacaacctg gcccgcttga
    83161 agaagaagcg tcagaccctc ctggaagcgt tgcccgggga tctggtgcgg cgctacgaga
    83221 caattcggga gaagagaaac ggcatcgccg tgatctccgt ctggaaggag gtttgtaacg
    83281 gctgccacat gaacattccg ccgcagatgt acattgaact ccaacgttcg caggagctga
    83341 ggacctgccc caattgccac cgcatcattt actggcggaa cgaagcgtca gtgggtgaat
    83401 cttgagtggc ggaccgtcac cagacggggg ggaggcctgc ggggtcgtgg tccatacgga
    83461 cggcgcctgc ctcggcaatc ccggacccgg aggatacggc gtcatcctga gttatcgggg
    83521 acggagcaag gagctctcgg gggggtatcg tctgaccacg aacaaccgga tggagctcat
    83581 ggcggtgatt gtcggtctgg aggcggtcaa gccgggctat gatgtgacgg tccattcgga
    83641 ttcccgctac gtcgttgacg ccgtgacgaa gggctgggcc gtccgctggc gggcgcaggg
    83701 ctggatgcga aaccggaagg atcgtgcgga gaatgccgat ctctgggaaa ggctgctcga
    83761 tctctgtaga caccgtacgg tccggtttct gtgggagccc ggtcatgccg gccatccgga
    83821 gaacgagcgt tgcgatgccc tggccgtggc ggcggccaag ggatccgatc aggccgtgga
    83881 cgccggatac gagcgggcat cccctcgcgg cgattccggt cgaaccgggg tgtgaacccc
    83941 cgcaacaggc attgaatgga gggggctgga gtggggcaga tggtcgctga ttctaacgga
    84001 tcagaggaaa gtccgaactc cacagggcag gatggtcgct aacggcgact gggggcgacc
    84061 ctaaggaaag tgccacagaa aagataccgc ccgcggcatg cgggtaaggg tgaaaaggcg
    84121 aggtaagagc tcaccagttt cccgggtgac cgggaaa
//
LOCUS       DAOOLJ010000004        82817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_140_length_82817_cov_17.564051, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000004 DAOOLJ010000000
VERSION     DAOOLJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 82817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 82817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82817
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_82817_cov_17.564051"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLW16_01320"
     CDS             complement(<1..659)
                     /locus_tag="PLW16_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB69573.1"
                     /translation="MKILIVDDEEGVRISLQKVLERDGYEVLLAERGADGIDLVRKFP
                     EDIEVVISDFKMPGLDGLETLMEIGRINPDIIRVILTGYATLDRAIDTVNQGIDGFLT
                     KPFENKEIRAKIREFITKKRLKQFVSEQVLQEMHRTGEKLLPKRRTVSVLFTDIRGFA
                     DMTARMSPQDIADLLNTYYFRPLDRIVFEYNGTLDKHIGDSIMAIFGAPLSYDDDAFR
                     AV"
     gene            complement(656..>1555)
                     /locus_tag="PLW16_01325"
     CDS             complement(656..>1555)
                     /locus_tag="PLW16_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB69574.1"
                     /translation="LILIAIAMVLIYWVFDSMASEQVFTRVVIVCLVIVYGVMTQSLI
                     NGRKAALAEKERTQEQLIQSESLAAIGQLVAGIAHELNNPLASASSLIQTDIELLEEQ
                     EEKRPIFEELRKDLAYSLKELNKTQRIVKSILDLSRQTQTYLEDVNMNAVIDEALQVL
                     YNQYKTMDVVIEKQYDPDLPALQGNFSNLGQVLINIINNALQALPEGRGTLTLSTARD
                     SKTESIVVTCRDTGVGIPPQTLKDIFKPFFTTKEVGKGTGLGLYISHEIVHKHGGEIH
                     VNSEAGKGATFTITLPCRRCGAP"
     assembly_gap    1556..1655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1991..2731)
                     /locus_tag="PLW16_01330"
     CDS             complement(1991..2731)
                     /locus_tag="PLW16_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPB69575.1"
                     /translation="MERKAYRYVDLITVLFVIVLVMSNILSSAKIVDWGVSLGGVPLA
                     FDAGTLLFPVGYIFGDILTEVYGFKRSRRVIWMGFFGLAVCSLLLYGVRILPGEALWE
                     QSVGQRAFDAVFGGMTSGGIVVASLLGYWLGEFSNSFVLARMKLMTRGRWLWTRTVGS
                     TLIGEGIDTLAFVGAATLFGVFPPALFVTLVVTNYLFKVGVEVLMTPLTYAIVGRLKA
                     AEGVDVYDIGTDFNPFRLRDDRTAAAKP"
     gene            2921..3856
                     /locus_tag="PLW16_01335"
     CDS             2921..3856
                     /locus_tag="PLW16_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPB69576.1"
                     /translation="MPSGKIFFVVNPHAANGETGRLWPLIQAKARERLGPFRAQLTAG
                     TDDATRITRQALLDGAELIVCIGGDGTLNEVVNGFMDEKGPIRPEAVLGLVPNGTGCD
                     FVRTVPIPADLDSALNTIRAGHTRALDLGRLRFADHRGGTGLRYFHNIASFGMGGEVV
                     DRVNRTSKALGPFISFMWGTLVSLMHYNAKRIRIKVDDYFDQEVAIWHVAVGNGRYHG
                     GGMLVAPDARMDDGLFHVTIVSDMILPRVFIHLPKLYNGKIKTIRQVSTLVGKTVSAT
                     SSDRVLLDVDGEQPGCLPATIEIVPAAIRMITQRG"
     gene            complement(4003..5016)
                     /gene="gap"
                     /locus_tag="PLW16_01340"
     CDS             complement(4003..5016)
                     /gene="gap"
                     /locus_tag="PLW16_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB69577.1"
                     /translation="MIRLGINGFGRIGRQVLKAVLTRYPGTLEVAAVNDLFDVRTNAH
                     LFKFDTSYGAYPGEVKVRKDAFVIDGKTIRNFALRDPAAIPWDAEGVDIVIESTGLFV
                     TGPKAAAHLEAGAKKVIISAPAKEEDLTVVMGVNHKAYNPKKHHIVSNASCTTNCLAP
                     PVKIVHEALGIEKGMMTTVHSYTNDQRILDLPHKDLRRARAAAMNIIPTTTGAARALA
                     LVLPDLAGRFDGYSLRVPTPTVSIVDFVAELKTPTTTEALRRLLANAAKKSLKGIMAC
                     EEAPLVSSDFKGNVHSSIIDLEFTQVLRDNMAKVVAWYDNEWGYSCRVADLADFMGRK
                     GWT"
     gene            complement(5030..5593)
                     /locus_tag="PLW16_01345"
     CDS             complement(5030..5593)
                     /locus_tag="PLW16_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB69578.1"
                     /translation="MKGKEMDYFTALYDVAKVINASLEPARVLDEIVRCVTETMRVKA
                     CSLRLLDAREKKLELGAAFGLSETYLHKGPIMVEESGLDQKALKGKTIWLKDAQTNRD
                     FQYGAKARSEGIKSVLVVPLLLEKKAVGVLRAYTDRVREFNDREVRFLEAVANLSAIA
                     LDNARMHRTLQYRCDLMAAHKNRIDDN"
     gene            complement(5942..6838)
                     /locus_tag="PLW16_01350"
     CDS             complement(5942..6838)
                     /locus_tag="PLW16_01350"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPB69579.1"
                     /translation="MPAFGIETLHRAVRVVIALVIIGAGAMSLPGQTSEPSASPLDKA
                     KEDLSRVIYPSQIRVPWRPHFLRPQDSLERLFGKDWVTVARFNRLDRRHAYPGMTIKV
                     PVDMIAARAYTPLPMRYEPAGPYPKYLLLDLTEQWLGAYEYGNLRLSMPAATGTADHP
                     TPLGFFQVTARDRTHTSSMYKTEKGEGQYPMDYALLFYVGPDGAAFWIHARDMPGRPA
                     SHGCVGVYDEFMQLRTYGTPEDPVLFDSRALYDWAVGDEKHGFDSGTQQEISGGPVIE
                     IRGELPRRLPRPTVLPPFQPPC"
     gene            7140..10082
                     /locus_tag="PLW16_01355"
     CDS             7140..10082
                     /locus_tag="PLW16_01355"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HPB69580.1"
                     /translation="MGRIGKITVGLVVVLGVIVIAGFYVLPPLMKPWAVEKLAGVLKR
                     DVAVERIRLNPFRLSCTVEGVMIREPAQTDPFVSFDGLYVNVGGLSSLFHRALVLEEV
                     RLTRPYVHIARKPDRTYNFSDLIPREKEPPEPEKKPFRFSLNNIALVDGSVDFDDRPM
                     NTRHTVRGMNLGLPFLSNTEVHVDHDVEPRLEALVNGDTLVLAGKSKVFHKSKESHFT
                     LNVRDLDLPHYLNYVPVKLNGTLVSAVLDTNLDIAFVMPEDQSPALRLGGDVTLRNVA
                     FDDPRGAAVIRLPSLRVDLAAVEPLVPDIHLAKVMLQSPEIAVRRDGNGRINLLNLTR
                     TPEKPAKREAPKAEEKAEQPAGKPALKLRIDEVAVEAAKLSFRDETPAQTADIRVAPL
                     DLRVANLSTEKGATGTVDLRLTVERQGTVTVAGPFGIEPLRADLALDVKKLAIRTFQP
                     YFADKIRIHVAGGTLSLAGNVNLSRERNRDPRATYAGKIYVSNLATLDQTHANRFVNW
                     KQLYFEGVRAGVNPFFTNIRGISLTDYYARIIINPDGTLNLTNILAGEKKETAGGAPA
                     EPKPAPAAQAKTAPAKAAMDIKIGKVTVQGGTIDFSDRFIQPNYSVKMLRMGGSVTGL
                     STDQASRAAVDLKGNLGRGSPVEIAGKINPLIKDPFVDMKLHFKDIELSPMTPYSGKF
                     IGHPIEKGKLTFDVAYRIDQRKLDAQNKVFFDQLTFGDRVDSPTAIKVPVTLAVALLK
                     DRKGEIHLDIPISGSLDDPKFRVWPVIWQVIVNLITKAATAPFALLSALMGGGEELSF
                     LEFDAGSARITETGRDKLKALAKALYDRPGLRMDIEGYVDVDQDREGLKKAELNRKLR
                     VQKLNDLIARGQSATLRDIGIEPGEYEKYLALAYRVETFPKPRTAVGVTKGLPPAEME
                     KLILTNIAISDGDLKALAARRASTVKDALLGSGEIPPERIFIVEPRSLAPRKQEKQKD
                     SRVDFKLK"
     gene            complement(10075..11559)
                     /gene="amrB"
                     /locus_tag="PLW16_01360"
     CDS             complement(10075..11559)
                     /gene="amrB"
                     /locus_tag="PLW16_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB69581.1"
                     /translation="MSWNRKQSSEEEGIRPPVVAGKFYPGDARVLRTAVEKFLADAAP
                     VQVKDAVAIVAPHAGYIYSGQVAADAFRQTAGGGYDLVVLLGTNHTRPNYRKIGFYPG
                     AGFRTPLGIAAIDQAAVSALIAADPDGIRDATVHEREHSVEVQVPFVQVLFPEARIVP
                     AVVGEAEPDLCRRFGEALGAVLRNRRALIVASSDLSHYPAAEDAERVDPAVLEAIAGM
                     DPARVRERIRTEMARRVRDLHTCACGEGPILAAMTAATALGAAAGRVVSYAHSGQVAV
                     GDRNRVVGYGAVAFAKGVVEMNAKPEAVGEEAGGLSMEDKKALLALARETITRLLTAQ
                     TVPLARGLSPRLEVRRGVFVTLKKRGRLRGCIGRMVGDMPLGQLVGTMAVQSAFHDSR
                     FAELRAEELKDLEIEISVLTPMTPVRGPGDIIVGRDGVLIRKGRHSAVFLPQVATEQG
                     WDRDEMLDNLAMKAGLSPGAWREGTEFLTFQAEVFGEAEFGGPT"
     gene            complement(11546..11752)
                     /locus_tag="PLW16_01365"
     CDS             complement(11546..11752)
                     /locus_tag="PLW16_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69582.1"
                     /translation="MPDRLFARVPRPRYIDAIKPAEKSVVFAGAGAAGQESREISRAH
                     MKGSNFRGNIMVEGFCIGGRHVVE"
     gene            complement(11788..12363)
                     /locus_tag="PLW16_01370"
     CDS             complement(11788..12363)
                     /locus_tag="PLW16_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPB69583.1"
                     /translation="MNVLLSGATRDGRTGLADGLADQFRRQDLLPGGFLCLPAERDGA
                     GPTYVIRDLLSGQARPFAHLHNPERAPAAGETAIGRWRVDAAGFAFGAQAVGRAVTLK
                     TPLVFLDEIGPLELAGGGFRWLADLLIRGPLKPPVFIGIVREEVVPRVRGLYPDQVFD
                     VVRAGARPAAEIYADLRRAMRGLGTQLRELR"
     gene            complement(12360..13211)
                     /gene="larE"
                     /locus_tag="PLW16_01375"
     CDS             complement(12360..13211)
                     /gene="larE"
                     /locus_tag="PLW16_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPB69584.1"
                     /translation="MTAGATTGQRAPEALRKRYGRLTDRLRKRGPCAVAFSGGLDSAF
                     LLLAAREALGDRVLAVTAETVYMAQWELEEAGEIARGLGVAHLRLDVAFPEAIRRNPP
                     DRCYRCKHHLMDLILAEARTRGFPTVMDGTNVEDAPEDRPGMRALRELGIVSPLREQG
                     WTKSDIRAMSRARGLTVWDKPPYACLLTRLPHGRVVTAKGLRRIEAAERHLAGLGFRA
                     VRVRSHGDLARLEAAPADLERLLDAELRRDVHRTLQGVGFRYVSLDLEGYRSGSMNRD
                     RKKREPE"
     gene            complement(13242..15029)
                     /locus_tag="PLW16_01380"
     CDS             complement(13242..15029)
                     /locus_tag="PLW16_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB69585.1"
                     /translation="MKIDLSGQDLQRRYSDLGARDDALLDSEGSFRQLLEDAHDCIVA
                     IDLEGRITYCNPAVQAMSGGRDLIGLPIRKLVAPESLSVYDTMIDRRRAGVTDHLSYA
                     FTLRDSMGAERHFDARSSILSKDGVPVGIMVIAREETERKKTEAVLVESEAKYRSLVE
                     NANEAIFIVQDGVLKFANPRTLVLTGATAEELAVTPFTEFIHPEDREFVLKSHLRRLR
                     GERFAPRYRFRAVRKAGETRWVELDAVRIQWQGRDATLNFVADVTERVAMEEALKESE
                     ARYRSFFMTSRDAVYITTREGELLDCNDAAAEMFGYGDKETLLRMFRISDIYAVSEER
                     ARHIEAIERQGSVMDYPITLRGLDGRIHHTLISSTLWRDPLGRVGFQGTIKDITERKE
                     AEEHLQKTLWNLRKAVEAIITTLSMAVEMKDPYTAGHQRRVSQLCRAIAEAMGLPPDQ
                     VEGVAMAAMIHDIGKIGLPAEILSKPTRLTPIELSLLQTHADTGYDILKDIEFPWPVA
                     HIVAQHHERMDGSGYPEGLAMDDILLEAKILAVADVVEAMASYRPYRPAVGIVSALEE
                     IERQQGILYDPEVVQVCLRLFREQGYRLE"
     gene            complement(15181..15417)
                     /locus_tag="PLW16_01385"
     CDS             complement(15181..15417)
                     /locus_tag="PLW16_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69586.1"
                     /translation="MGKRYELIYGYLHCIGRTTYSAGFVATEDEARAWVERQQAPGAR
                     RMKPPQEDPIRRCGVSYCPLKAQQPWFAWRVCEE"
     gene            15587..16423
                     /locus_tag="PLW16_01390"
     CDS             15587..16423
                     /locus_tag="PLW16_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB69587.1"
                     /translation="MYEDLKGKTALITGSGKRTGMGYAMAETLAGAGTNVIIADLGRG
                     EVIDGDLKMGAWEEMTRLAEELAERFGVATLAVAVDVTDNASIAAMVATVRERFPRVD
                     ILCNNAGASFGVPNAVLTYDEAAWLKTIDVNLHSVFRISRAVIPLMAGAPGVIINTAS
                     RAGKVPPIFNGAYAVAKAGVIMLSKVMAKELGGMGIRVNAICPGQIRTDLEKWRFGLE
                     AKFFATTVEEREQEMCKTIPLGCLGIPADAGHLVAFLASDASRYITGQAINLDGGQCM
                     EL"
     gene            16453..18150
                     /locus_tag="PLW16_01395"
     CDS             16453..18150
                     /locus_tag="PLW16_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPB69588.1"
                     /translation="MDKIIGGKLVADALVDWGVDYIFSLSGGHITPIYQFLENTNITI
                     FDTRHEQAAVFMAEAWARMTRKPAVAMVTAGPGFTNALSGIASARLSNAPVILISGCV
                     GTEATEKLDLQDMSQLPVITPMVKKALVCHIPERIPEFIDMAFRTAIAGRPGPVYLEL
                     PCNVLNATVNPALVKKMHTTLSSQPVDRENTRKVVDLLKAAKNPIVIGGSGIWYGGAE
                     KEMIAFVEKIGIPAFTAGAGRGVIPDTHPLCFGSSLAIRPGAAMATLMTTDLVLFLGG
                     RLSLFYIFGEIFPPNAKFIQVDIAPDEIGRNRTVDLGIVSDTRAFLRELNGMLDAEGI
                     AETLKKQYQPWVETVRKADQDGKNGAKALWECDVQPIHPMRLAKEVNDFMNREDDIVV
                     ADGGDTSTWTGMTRTVVKGGTYLDYGLYGCLAVGIPYANAAKLKHPDKRVLLIMGDGS
                     VGFNFMEFHTAIRKKLPIVVVIGNDQSWGMIAHSQQLRLGHNIPNGTELGWVDYHKMV
                     EALGGFGTMVEKPEDIRPALEAAFASGKTACINVKVDPSVISPGSVALANLGGYKCE"
     gene            18226..19524
                     /locus_tag="PLW16_01400"
     CDS             18226..19524
                     /locus_tag="PLW16_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB69589.1"
                     /translation="MNHDAVAPEGSKLSNRSAYYIFTLLFLLYFFDYVDRTIVTSVAP
                     FIREEWDLTDMQSGLLLSVVYWSIVAFVFPVSILVDRWSRKKTVGVMALLWTFATVAC
                     AFTKSFPQLLLARGAIGVGEAGYAPAGTAMLSGLFPPEKRSRMMGFWNIAIPLGTAVG
                     IGLGGFIATHWGWRHAFGLVALPGAIIAILFFFVKDYKTVELVKTVGAHDTGKNKIQM
                     SKMDIVREFIHTPSLMLTNVGFIGCIFVNNAIIFWMPTYFHRTGGLSISEAGMKTSLL
                     MVLALVGLPLGGWLADMWFKKRVSARLLFPAITTALNAVAIFIGISLLTGQAQYVTLL
                     LMGVLASAFAPAAITATQEAIHPGLRAISYSLCVVVQNLFGASMAPIVIGKLSDLYGI
                     QTAMSVLPVFLVASALVFLAGSFFYARDLAKVEKVTLEWE"
     gene            19563..20222
                     /locus_tag="PLW16_01405"
     CDS             19563..20222
                     /locus_tag="PLW16_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB69590.1"
                     /translation="MGSKERREREKEQRKSQILDVARELLLEKGLQATSINQIAKRSE
                     LSVGTFYFYYRSKEELYVALQQEGLDLLYGMTTAAIRRKRNPRAKIHAIAQAYLTFAE
                     EHRSYFDIINFFLNSSSEIFFSEQLKEQVDEHGNRIVDVLANVIADGIRSGDFRPVPA
                     KRYALILWAMIHGVTQLRKLRHTILRGEDLHKIYTDAVGLFIDAIGTAGAGQEAPTLD
                     T"
     gene            20284..21483
                     /gene="dsrA"
                     /locus_tag="PLW16_01410"
     CDS             20284..21483
                     /gene="dsrA"
                     /locus_tag="PLW16_01410"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HPB69591.1"
                     /translation="MTDSKTPLLDELEKGPWPSFVKEIKQAAEKKPAAADLLNQLELS
                     YKDRIGHWKHGGIVGVKGYGSGVIGRYSDRPDLFPNVQAFHTMRVNHPTGWFYKTDAL
                     RKLCDIWDKHGSGLTNLHGSTGDAILLGTTTEHLQPCFDDLSEAGFDLGGSGSALRTP
                     SACVGPARCEWACIDSLDICYQATMTLQDQIHRPMFPYKFKIKVSACANDCVSAIARS
                     DLAIMGTWKGRIQVDPAEVRAYADAGLDIRGEVCDLCPTKCMEWSEADRTLVIHDGDC
                     NRCMHCLNVMPKALRPGKERGASILVGGKAPIVQGSLLSWVIVPFMKLEPPYTELFDL
                     IARIQDWWDENGRSRERLGELINRLGMRHFLKAVGLPAVPQMVKHPRTNPYVFFWPEE
                     IDKKEVK"
     gene            21485..22546
                     /gene="dsrB"
                     /locus_tag="PLW16_01415"
     CDS             21485..22546
                     /gene="dsrB"
                     /locus_tag="PLW16_01415"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HPB69592.1"
                     /translation="MAKTDIGPPDYKQFLPPIIRDNYGKWRYHEIVRPGVLKHVGENG
                     DVLYTVRVASPRLLSTDHIREICDLAETYCDGFLRFTSRHNIEFLLADEHRVAPLIEE
                     LQAKNYPVGGTGHSITNMVHTQGWVHCHTPATDASGPVKAIMDELYDHFTHMDLPAHV
                     RISLACCLNMCGAVHCSDIAILGIHRTPPRPDNSRLPNVCELPTTVASCPTGAIRADM
                     KNKTVQVNDEKCMYCGNCYTVCPAMPIADPDNDGISIWVGGKVSNARSAPMFSKLAIP
                     FLPNNPPRWPEVVAAVKNIVEVYAAHARKHERLGEWIERVGWERFFTLTGIAFTDKHI
                     DDFTLATETFRTTTQFKWG"
     gene            22548..22775
                     /locus_tag="PLW16_01420"
     CDS             22548..22775
                     /locus_tag="PLW16_01420"
                     /inference="COORDINATES: protein motif:HMM:NF020263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HPB69593.1"
                     /translation="MSAEIKERVLAYFREKSKGDKKMFYIKDVVKGLPDVDRHAVQAA
                     VKELLDEEVLKYWSSGSTTYLMLAENFPKDA"
     gene            22835..24409
                     /locus_tag="PLW16_01425"
     CDS             22835..24409
                     /locus_tag="PLW16_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HPB69594.1"
                     /translation="MDRTQTVWPTSLVSAAVPRLVIGALRGGAGKTTLAVGILAALRR
                     RGLDVAPFKKGPDYIDAAWLSAAAGRSCRNLDTFLMGADRVRSSFRQYAPPSGFALIE
                     GSRGLYDGLDAAGSHSTAELAKLLTAPVVLVMDSDKVTRTAAAMILGCQRLDPAVDIR
                     GVILNRTARSRHEGILRRAIEEACGIPVLGAVPRMADYPFPERHLGLTPPQEHDHVTG
                     ALRRAAKVAEDCLDLERLVSLTESAPPLAAGNADLADGARRPGTAHRRRRPETRDSGR
                     PVIGIIRDSAFQFYYPENLEALAARGAFIVEISACTAAALPEIDALYIGGGFPETHAR
                     ILAENKRFRQALRQGVEDGLPVYAECGGLVYLGETLTVGTETFPMAGVLPVAFGMERK
                     PQGHGYTLLEVTEVNPFFPVGTTLKGHEFHYARVLDGDAGGATPCCRVIRGRGIDGRG
                     DGLVSNNCLALFTHLHAAGEPRWAEALVKQAVRHRRTRSRRERDANRAARGPAAFLEG
                     PAGIGLPRGTAPQTQQ"
     gene            24436..24756
                     /locus_tag="PLW16_01430"
     CDS             24436..24756
                     /locus_tag="PLW16_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPB69595.1"
                     /translation="MPTIELAGKSYEVDEDGFLQELDKWSEEFAEAYAHVDGIEGPLT
                     EEHWRVINYLRGYYQEFGIAPMVRKVVRETGLDLKRIYELFPSGPGNGACKLAGLAKP
                     TGCV"
     gene            24871..25812
                     /locus_tag="PLW16_01435"
     CDS             24871..25812
                     /locus_tag="PLW16_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPB69596.1"
                     /translation="MGLYVLTYVCALVFVLAMGLRIRRQIALPVHVRWELYPVRHETA
                     ERVAYGGSYLEALNWWERPDERSVWNELKYMAPEILLMRGLWKDNRRLWWVSFPFHFG
                     LYLMTATFGLLLLHALLALVGSQDLAEGHPLGRLLDVLIVLAGWTGLILGNIGCLGLF
                     HRRLADPELRDYASFADYLNLGFIFLFFLCAFAAGLGDPSLAGARAYALGLLTAGSAP
                     AASVPGPSAAGIATVVMASLLAAYIPLTHMSHMLMKYFFYHNVKWDDAPNRRGGKIEA
                     AVRKNLGLRPTWQAWHVGADGRKSWREIAASEPGEMK"
     gene            25809..27422
                     /locus_tag="PLW16_01440"
     CDS             25809..27422
                     /locus_tag="PLW16_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB69597.1"
                     /translation="MRTRLAPTDISRPSDCLSHIEAEDLAPLPPPYEDFNTTAIPPLK
                     PEKVRHIETSLDDTVALGIPKPASPEEEEALVRQFLSGLKKLFEKENNWTFLQPLLLS
                     MEHCAKCQTCADACPVFEMSGRKEIYRPIFRAEVLRRIYKKYLRPGGKITAKLTGDID
                     LNWTAIARLAELSYRCTLCRRCAAACPIGVDNGLIAHEIRKLFSQEMGIAPKELHEKG
                     TVQHLQVGSSTGMKPPGAKDSLEFIQEEMEEITGYKVDIPWDVEGADILLIHNAGEIL
                     AWPDNVGAFAVIFQAAGVSWTLSSEAAGYDVVNYGLWYDDVQFSRVAVRHADIARRLK
                     VKKIVIGECGHAHKALTVVADRLLTGDLNIPRESALVTLADIVLSGKLRLDPRRNDFP
                     VTLHDPCNIVRSMGIVAPQRRILKKIAPRFREMTPHGVENYCCGGGSGFAIMSGHNFV
                     DWRNRVSSRKKFLQILAAFANEPAEVPKYVCAPCSNCKGAIRDLIRYYHAEERSRLYY
                     GGLVDLIVNAMADMKKPIIRFGDEPSWKA"
     gene            27454..28491
                     /gene="dsrM"
                     /locus_tag="PLW16_01445"
     CDS             27454..28491
                     /gene="dsrM"
                     /locus_tag="PLW16_01445"
                     /inference="COORDINATES: protein motif:HMM:NF038037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HPB69598.1"
                     /translation="MTLFAPLIAIALLIALPLAGLAASGEGLLAVGVPYAALAVLVVG
                     FVRRIARWANAPVPFRIPTTAGQGRSLDGIKPDRLDNPATTWGVLGRMALEILCFRSL
                     FRNTKQRLAPPARAESAGDKAAATARPVYVSAKGLWFFALLFHGSLLVIVLRHLRFFL
                     TPVPTWVDGLIALDGLFAVGLPTAYLSDAALLAALLFLLLRRVLIQQVRYITLAADYF
                     PLLLIGAIAVSGLLLRHAFKVDLNQVKALAVGWATFSPAVPAETEAFFFVHLFLVCAL
                     AAYLPFGKLMHGAGVFLSPTRNLANDNRRRRHVNPWNAPVKVHPYEEYEDEFRGKMKA
                     AGIPVEREEGS"
     gene            28564..30201
                     /locus_tag="PLW16_01450"
     CDS             28564..30201
                     /locus_tag="PLW16_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB69599.1"
                     /translation="MAIDKLPTPEELSHIDYRPPGTGWRNTPVDFRPGTWCYSATPKS
                     LTTLDLPNPRQWQPSDADWKLPENWRTIILDGMRERLEKYRSFRLFMDICVRCGACAD
                     KCHFYIGTGDPKNMPILRAELIRSVYRRYFTTAGRILGPLAGARDLTEDVLKEWFYYF
                     YQCTECRRCSVFCPYGIDQAEITMIGRELLTLVGCNINWVLEPAANCFRTGNHLGIPP
                     HGIRDMLDFLVDDIKDRTGVRVEVPLNRKGAEILFVTPSGDYFADPGTYTCMGYLMLF
                     HELGLDYTMSTYASEGGNFGLFTSHEMIKRLNAKIYAEAKRLGVKWILGGECGHMWRV
                     VHQYMDTMNGPADFLEVPVSPITGTRFENAAGTKMVHIAEFTADLIQNGKLRLDPARN
                     DHLRVTFHDSCNPARAMGLLEEPRTIIRHACNHFFEMPEDTIREKTLCCGGGAGLGTD
                     ENMEMRMRGGYPRAMAVKHVRERHGVNRLACMCAIDRVTLPPLMDYWVPGVGVTGVHE
                     LLGNALILTGEKQRTTDLRGEPLLRASHADGVHEAKG"
     gene            30263..30769
                     /gene="dsrJ"
                     /locus_tag="PLW16_01455"
     CDS             30263..30769
                     /gene="dsrJ"
                     /locus_tag="PLW16_01455"
                     /inference="COORDINATES: protein motif:HMM:NF038038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HPB69600.1"
                     /translation="MKGTDRWKIFAGLFVFLALAALPFWYGGWDRPAPVLDLDTPAIR
                     QLPERRCVEATAFMRANHMTLLADWRDASTRDGRRIYVARDGREIEASLSQTCLRCHT
                     DKTRFCDRCHDDAGVSPTCWDCHVVPEDRRESATVTHRQRGEGGDVSPFPVDVAAREK
                     ESRGPGPR"
     gene            30794..31573
                     /locus_tag="PLW16_01460"
     CDS             30794..31573
                     /locus_tag="PLW16_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB69601.1"
                     /translation="MHSSRRDFLKLTGIALIGAAVPDALLPAGLAPAWAGAPGFRPDV
                     RALKAARWGMVIDLAKCRPDCTDCIAACHAAHNVPAFGNPKDEIAWIRRETFGRIFPA
                     QVHRHLEPRRQAGPVIALCNHCANPPCVRVCPTGATFRRPDGIVMMDYHRCIGCRFCI
                     AACPYGARGMNYRDPRPFIRRIDPGFPTRTRGVVEKCNFCVERLAVGKPPACVAACPY
                     GALTFGDLEAPDSAVRRAIAGRTVLRRKPELGTEPQVYYTL"
     gene            31595..32752
                     /gene="nrfD"
                     /locus_tag="PLW16_01465"
     CDS             31595..32752
                     /gene="nrfD"
                     /locus_tag="PLW16_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPB69602.1"
                     /translation="MLEKALHGNHRYWGWLAFLAVVVGAGAFFYLRQLDYGLGITGMS
                     RDVTWGLYIAQFTFLVGIAASAVMVVLPYYLHDYKAFGRITILGEFLAVAAVVMCVTF
                     IVIDLGRPERAFNVLLHPAPRSILFWDMVVLTGYLVLNLVIGWTVLENERNGKAPPAW
                     VKPLILLSIPWAISIHTVTAFIYAGLPGRDFWLTAIMAPRFLASAFASGPALLVLLCL
                     VLRRWGGFDAGREPLQALARIIAYALAASLFFVLMELFTAFYSGIPGHTEPFRYLYAG
                     LDGHTRLVPWMWTSAVLAVLALVLLIVPALRRNEAWLALACLAVFASLWIEKGLGLVV
                     TGFIPSPLGQITEYTPTGPEVAITLGVWALGFVILTALYRIALGVRKETEA"
     gene            32759..33961
                     /locus_tag="PLW16_01470"
     CDS             32759..33961
                     /locus_tag="PLW16_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB69603.1"
                     /translation="MKTLYVETTPARFLAVKTLRGLWPGVLWSRLSTLQYREMTPTAP
                     PAGWVHVKNHQGGICGSDLHLIFLDADLSVHPALLPGSTRKYLGHELVGHVLEAPPDS
                     GFKPGDRVIRQMRTGGGSCLAHGLAPCGFCRAGQYHQCERTGLPDSIGGGLSDELYLP
                     AGGLMAVPDDLADAQAVLVEPAACAIHGVLRAPPNPGEAVLVYGAGTIAYFVLQVLRI
                     VLPDCRITAIAEFPFQEDMARRCGADEVWLAHDDLLARTAARTGGRVVTGRANRRTLL
                     GGFDRIYDCVGVAATLGLSLRLARARGTVILIGVTLKPLTLDMTPVWYNEVNLVGSVS
                     FAAEAWRGERIRDFDLAIRWMREGRLKTEGFATHRFPLERYREAILAAVRKASSRSVK
                     VVLEMEKS"
     gene            34034..35263
                     /locus_tag="PLW16_01475"
     CDS             34034..35263
                     /locus_tag="PLW16_01475"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPB69604.1"
                     /translation="MNTKMAMKILVLNCGSSSVKYKLYDMESGRVVALGFAERIGLAG
                     SGIRHQAEGAAAVSFEHTLRDHREALEILLGMRPGGPRAGSIDPAAITAIGHRVVHGG
                     EYFRTPVIIDDAVLVNLKACSMLAPLHNPHNLAGIEVCRKLLTEVPQVAVFDTAFHQT
                     MPPLAYTYAIPHRYYDTYRIRKYGFHGTSHRYVAERAAELAGRPLAELKIVTCHLGAG
                     CSLCAVRDGHSIDTTMGFTPLAGVVMGTRSGDIDPALVPFIADKEGLPPAAVMDILNS
                     ESGVFGISGISGDFRDLLKAAREGNERATLALDVFTYSIAKGIGSLLPALEGLDALVF
                     TAGIGENSPDIRRLICRRLAYFGITLDDGKNALARTETDVATTESRVRIYVIPTDEER
                     MIARETRKLIDPGRGPG"
     gene            35350..37104
                     /gene="xsc"
                     /locus_tag="PLW16_01480"
     CDS             35350..37104
                     /gene="xsc"
                     /locus_tag="PLW16_01480"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HPB69605.1"
                     /translation="MAKIKMTPSEAIVETLLAEGVDHVTGIVGSAFMDMLDLFPTAGI
                     RFIPVRHEQSAGHMEDAYARITGRAGVLTGQNGPGITNMVTSVAAANMGHTPLVIISP
                     SAGTPTIGWDGFQECDQVSVFKAITKATVRVPHPKRAADCLRTAFRIAYADRGAVLYD
                     IPRDYFYGELEEEILPPHRYRVDARGCGSLADIERAAKLLMNAKNPVIVSGRGVVDAD
                     ALDVIKAIAEHLTAPVAVTYLHNDAFYGDHPLFVGPIGYMGSKAAMRTLAKADVMLAV
                     GTRLSVFGTLPQYDINYFPKKAKIVQVDINPKHIARTHPVEIGIIGDAKEAAVEILKR
                     LRTADKRRKPDTARLGKIAAERKKWEQEIVRLARVKGTPINPRRVLLEMSRMMPDNAI
                     LTTDIGNVSSTANSYFTFKGGRRHIAALTFGNTGFAYPAALGAQLARPKAPVVAIIGD
                     GAWGMSLHEISTAVEHNLPVIACVFRNMWWGAEAKNQVDFYNNRFIGVDIPNPASFIP
                     AAEALGARGIRVEHPDQIGEAFKSALKIRRPTVLEFVVDGRQLAPPFRKDALALPTRL
                     LPKYAHLDHHNWRTDYDM"
     gene            37471..38013
                     /locus_tag="PLW16_01485"
     CDS             37471..38013
                     /locus_tag="PLW16_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPB69606.1"
                     /translation="MLYEFDGRTPQVADGAYVSDTATVIGDVVIGPHCYIGHGAILRG
                     DYGRIEIGPGTAVEEGVIIHAPPERVCRIGEKVTLGHGAVIHGQRLGDCSVVGMGAVL
                     SILSEIGEWTIVAEGAVVKIGQAIPDGMVAVGNPAAVVREIADRDREMWSWGKQIYID
                     LAEKYLRVGLHPVAPDGRNG"
     gene            complement(38089..39567)
                     /locus_tag="PLW16_01490"
     CDS             complement(38089..39567)
                     /locus_tag="PLW16_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPB69607.1"
                     /translation="MMNRENKRPVLTRRDLLKAGGRVLGAGFAGYAAYGLLGDRLGAT
                     ERPLGEKAKAGIPLVRSRGRRGGNRPNILFILGDNHNAKTMGCAHHPFIQTPGMDRLA
                     REGVLFEQAFNTTALCSPSRASILTGAYAHRHGVRNNHTAWTGRFTTFPEYLSREGYA
                     TAFIGKWHMPGGLPHLPFLDLFVAYTYREGQGAYFNCPMIVNGREVPSRKPYITEEVT
                     DYAIEFIDQQRSRNDGRPFCVYLAHRPGHPPFQAPADIAGMYHDADVSRVLPPNVDPI
                     WFGKTRGNVFQGIMMGSYEDQYRRYCETLTAMDRDIVRILDYLDRHNLSEKTIVVYMG
                     DNGMQWGTHHCHGIREPYEDSIRLPFIVRAPVIIHDPGGRRHQMILNIDVAPTMLDLA
                     GLEVPGDMDGISLAPVLGNPRGKTREACLLEFWRYYPENTPSYRGIRTENFKYIEFER
                     GRRPWLFDLKSDPGEMNNLFGRAEMSDIANELKERLAGLPRG"
     gene            complement(39609..40172)
                     /locus_tag="PLW16_01495"
     CDS             complement(39609..40172)
                     /locus_tag="PLW16_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69608.1"
                     /translation="MGRKSFILRVGMPVLPVFVAALLSFAVYFFLRFPDRTTFTLVVA
                     TLSGFFYFVSISFGALYVYTVSQLRGASLAESVLASFVTPFAWITKEVLILTESHPVL
                     ECLYWYLSPLSIWLFVLMCAELGVAALIVRKIRIQRNEKAYSMRVPALIIAGSLTVFA
                     LMYAWGRGENIYVIFLEGYRFFFGSGL"
     gene            40552..41880
                     /locus_tag="PLW16_01500"
     CDS             40552..41880
                     /locus_tag="PLW16_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB69609.1"
                     /translation="MIQIALPNKGASRDGVMRELKALHSADNDWRTGRMFSLIYNAGA
                     DVESVAREAYAEYMVENALSPFSFPSLLKMETELLSMIATLFHGETAVGSMTSGGSES
                     ILMAVKTAREWARAKRPGISSPELVMPVTAHPAFNKAAHFLGIRAVAVRVDGGFRASV
                     PDMIGAVTENTIMMVGSAFTYPHGMIDPIGELSGFALENNIWFHTDACLGGFMIPFLE
                     MEGYPVRPFDFRLPGVKSISADIHKYGYTPKGASTVTYRDMDLREFQFFAYADWPGGV
                     YGTACVSGARPGGAISSAWAVMRYLGQEGFRGLASKAMRAAKRLRAGIEAIPGLHVLG
                     NPDGTVFAFGSDGLNVYDLRDALKRRNWHVESQHLPPCLHLTVCPVHEEVVDAFLEDL
                     AEACGEAASVSENDRTEDAVLYGVMGTIPDRSQALDLAVKYLNDLYRIKP"
     gene            41898..42608
                     /locus_tag="PLW16_01505"
     CDS             41898..42608
                     /locus_tag="PLW16_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB69610.1"
                     /translation="MIGFLVIAHAVESVLGFGATVIAFGLGAHFFPADRLVVILVLIA
                     LVQSAYLMARWFRDIEWGIVFRSIAPAALMGLAAGILIRSMVNEGMLKLILGGFIVTL
                     SLIELRFLFLKKDAPPRDPSAVTGSLLVFGGGVFHGLLAVGGPLIVYYAGRKLASQES
                     VRGTLAFIWIFLNTIMLAGFIADGSITADVLSTAGLLLPGVLAGILIGNRLKISDRLF
                     KTATYLLLLVVGATLVLP"
     gene            42649..43824
                     /locus_tag="PLW16_01510"
     CDS             42649..43824
                     /locus_tag="PLW16_01510"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPB69611.1"
                     /translation="MSLILLSLTFLLFPAVVIYACGRFPAIDKIGAGIICYGAGIALS
                     LAGLVPAGARPVQEAFMNVSVPLSIPLMLFSVDLKRWSRLAGTTATSFACMILAVLCA
                     TAAGFLIFEDRLPEVWMIAGMSIGVYTGGTPNLNAIGLALKAPEHIIVLANTADMLVC
                     IPWFIFILTFAQRILNLFLPPFEKHLASPAADGRSAPDTDWETKDINDYRNIFSRPVL
                     LPLTGGLCLALTIFAAGVGLYSIAPREYNMAVLMLAITTLGLAASFIPAVRNIDKTFQ
                     LGQYIILVFCLVVGSMADIRQLVIAAPHIILYMALVVYGSFALHLFMSRIFKVDTDTT
                     IITATAAIFSPPFVPVVASALRNREVVISGLTAGIMGYVIGNYLGITIAYLLRAWGG"
     gene            complement(43955..44398)
                     /locus_tag="PLW16_01515"
     CDS             complement(43955..44398)
                     /locus_tag="PLW16_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB69612.1"
                     /translation="MQRASDLLKGKSKQLWTVLPEASVLDALKLMAEKEIGALVVMDK
                     KEKVVGIFTERDYARKIVIKGKNSLDTRVKEVMTPFEKMYTVKPDTRVEDCMVLMTGK
                     HIRHVPVFDGPKFLGLISIGDAVKAQIAQKDALIDHLSDYIGGKY"
     gene            44630..45154
                     /locus_tag="PLW16_01520"
     CDS             44630..45154
                     /locus_tag="PLW16_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:45026..45028,aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPB69613.1"
                     /translation="MSVMRAKTWSPYVAGALAGLLLVLSVFLTGKYFGASTTFVRAAG
                     FVEQSVAPEKVAGMEYFLKEKAKLDWQGMFVLAVLFGSLAAASLSGDRRAVPVPAMWE
                     ARFGASRVRRWTLAFLGGVIVMFGARLADGUPSGHGLSGLAQIAISGYISLVCFFLAG
                     IVVARILYGGGQRP"
     gene            45151..45678
                     /locus_tag="PLW16_01525"
     CDS             45151..45678
                     /locus_tag="PLW16_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPB69614.1"
                     /translation="MNELLTGAVTGLLFGFIMQKAQVIRYDRQLGALRLQDMTIVKFM
                     LSTILVAMVGIYLLFDLGMVKLSIKSTILGANIVGGLIFGIGWGLVGYCPATAMAALG
                     EGRYDSLFGLLGMLLGAGLFAEAYPFLKATVLTWGNFGKISIPAALGVNHWIVIVILV
                     AAFVGLFYWFEKKGL"
     gene            complement(45732..47162)
                     /locus_tag="PLW16_01530"
     CDS             complement(45732..47162)
                     /locus_tag="PLW16_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HPB69615.1"
                     /translation="MRTSRWAVAFSLLSGIVLASAALAGHVELVGKGPFKSGPEVTKK
                     CIECHEKETRDFMKTVHWTWTKEQKWKGKTVNIGKLNALNNFCIGLPGNWPRCTSCHA
                     GYGFKDESFDFSKAENVDCLVCHDTTGAYKKFPTAAGHPAYAGEAREFPKGTPWPPAD
                     LLKVAQSVGPTSRTTCGACHFYGGGGDHVKHGDLDTSMAHPSPEIDIHMGGKSKMTCQ
                     ACHQAKDHFLKGEAASVTLTHTHPSHIDCVDCHTGNVHRNFLVNKHTERVACQSCHIP
                     TFAKARPTKTWWDWSTAGKDIKAEDIPKDQYGMKLYDKMKGDFKWDKDVMPTYLWFNG
                     NIERYHAGDKIDPTKAVKLTHVTGGRNDPHSKIYPFKIMRGKQAYDRGNNTIAYVRVF
                     GPPGSDAYWQKYDWDRAIASGMKAAGQPYSGRFGFVETTMIWPVHHMVVSRDRSLKCG
                     DCHGENGRFDWKALGYKGDPRDPKNR"
     gene            47491..47838
                     /locus_tag="PLW16_01535"
     CDS             47491..47838
                     /locus_tag="PLW16_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HPB69616.1"
                     /translation="MKKFALFVFKGDPLCFIHVLLNAMDMKAKGHEAKIILEGAAVKL
                     VPELVKANNPLNGLWKKSLDAGLVEGVCKACSNKLGTLEAAKAQDLTLLDDMSGHPGM
                     ASYRDKGYEIISF"
     gene            complement(47933..50017)
                     /locus_tag="PLW16_01540"
     CDS             complement(47933..50017)
                     /locus_tag="PLW16_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB69617.1"
                     /translation="MSEELRWVKTHCARMDHGGCGLRVGVRGNEIVRIEGDPDGYLNR
                     GYTCFKGRVSAERLTHPDRLKQPLKRVGGRGEGKWQAISWEQALEETAENLRRIRETY
                     GARAVGFGVGMPKGLEHFVLIRLANLFGSPNVVASQDVCHAPREITGVHTCGFYPVAD
                     LHHPTELMLLWGSNLTSTHEEGQIAVQLLERVKGGAKLVVVDPRRIELAEKADLWLPL
                     RPGTDPALALGMLHVICEESLYDKAFVETYTYGFEDLVRQVKAYPPERVAEITWVPAE
                     QIRRAARMYAAAKPAALQWGNAVEHSVNAFDAIRALVCLMAVCGNLDVSGGNVNARDP
                     KIMGLGPFVRADLIPDKRKEMIGAHHGIIPRLMTVAPAFFRQAVLKDVPYPVRGFYGM
                     CTNPMVAWADSRRTFETFKRLDFVAVADLFMTPTAAMADIVLPVAHQYEMNDIGHYGL
                     GHGVIFARPKIVEPPEGCWPDMKILNELGKRVSSPELWPEDHEAFLEDVVRPAGLTYR
                     QFVERGYLKGPDRFRAYEEKGFSTPTGKVELRLSTAGKFGLTALPEFSGFPEEDDPDY
                     PLLLISAKSRYFLHSSYRWIRRLRERRPHPTVEIHPETAARHGIGDGERVVIETRHGA
                     MTQRARLTDGLDPRVICASPGWWFPEGDPARQYDWGEANVNLLTSVEKLGREFGTPNL
                     KGLPCRLLGPRT"
     gene            complement(50142..51098)
                     /locus_tag="PLW16_01545"
     CDS             complement(50142..51098)
                     /locus_tag="PLW16_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69618.1"
                     /translation="MKKIALNALDRVEILTLQDNTIDITMMDNSAVVQRAMAIKEGQI
                     RNSILAEHGFSALVKTTAAGKTRTLLFDFGYSEEGAAYNARALNADLTQVEALALSHG
                     HSDHMGGFAALTAMIGKKGLPFYVHPGIFKTPRYVKYGEGLKFDFPTITRESLEQAGV
                     NVVETKAPVTLLDGDVGFLGEIERVTDFEKGFPIAFFQEDAVEKWDPIEDDTAVVMNL
                     KGKGLIVLSGCAHSGVVNTVRHAQNVTGIEKVHVVMGGFHLSGPFFEPIVGRTTEELR
                     KLAPDYVIPTHCTGRKAILYMENTMPEQFILNMSGTKLTFAA"
     gene            complement(51138..51710)
                     /locus_tag="PLW16_01550"
     CDS             complement(51138..51710)
                     /locus_tag="PLW16_01550"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPB69619.1"
                     /translation="MTKTQGVEKRTMVPKRIFLTKGVGSHREELHSFELALRDAGIEK
                     CNLVQVSSIMPPGCRLITRPQGLQEIKPGAITYCVMSRCCSNEPRRLLAASVGLAIPK
                     DRQAYGYISEHHAFGQTERQAGDYAEDLAAAMLASTLGIAFNVDESWDEKKEIFRISG
                     KIVATKNITQSSIVKSKGYTTVLAAVVFVL"
     gene            complement(51707..52168)
                     /locus_tag="PLW16_01555"
     CDS             complement(51707..52168)
                     /locus_tag="PLW16_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPB69620.1"
                     /translation="MEEELMSAEERLGLARTEDQPRYGEKLLYYGNSAEAVGIMDDPD
                     AHGRVEGFCGDSTEMFLAIRDGVIAEARFTTSGCFFSRAACHAAVALAKGKTVAEALK
                     IDENAILAELGGMPEDHEHCAYLASLTLRWAVEEWQEKRGPVGRARPDAAR"
     gene            complement(52244..52966)
                     /gene="prxU"
                     /locus_tag="PLW16_01560"
     CDS             complement(52244..52966)
                     /gene="prxU"
                     /locus_tag="PLW16_01560"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084511687.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(52712..52714),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPB69621.1"
                     /translation="MGKEIKAGCARPTGGPVGEKADTSKDSPQQPMIKEVKAMITVGK
                     KAPDFTAPAYHKGKFISVQLSEFLGKWVSLCFYPGDFTFVUATEISAVAEKYPEFKKL
                     GVEVLSMSVDSVFVHKMWNDHELSRMVKGGVPFPMLSDGGGRVGSVYGVYLEDAGVEA
                     RGRFLIDPDGVIQGFEVLTPPVGRNVGETIRQFQAFQHVRKSKGTEAMPSGWKPGKLT
                     LKPGPGLVGKVWEVWKTKMAFD"
     gene            complement(53161..53421)
                     /locus_tag="PLW16_01565"
     CDS             complement(53161..53421)
                     /locus_tag="PLW16_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB69622.1"
                     /translation="MPTYEYECAECGLKFERRQAITEAPLVECPACGGGVRRLISGGT
                     GFILKGSGHGKAGRGGGCALEETGRTCCGRDARCGSASCGDE"
     gene            complement(53499..54755)
                     /locus_tag="PLW16_01570"
     CDS             complement(53499..54755)
                     /locus_tag="PLW16_01570"
                     /inference="COORDINATES: protein motif:HMM:NF041136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140132 - iron-sulfur cluster carrier
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein MrpORP"
                     /protein_id="HPB69623.1"
                     /translation="MDCQGKETGRQGHQQRPDAQDRKERREQEALTERMNQIKHKIMV
                     LSGKGGVGKSTVAANIAVALALEGRKVGLLDLDFHGPSIPKLLKLEQGQLRSDGKSIV
                     PVEYLYGIKVVSIGFIMPERDAAVIWRGPMKMGAIKQFLSEVAWGELDDLIIDFPPGT
                     GDEPLSAAQLLPDSDGAVIVTTPQDLALSDVRKSISFCRQLKLPVIGVIENMSGLVCP
                     NCRTVIDVFKRGGGEAMAREMGVPFLGRIPLDPAIVEASDDGNPFIYFQNKTETAQAF
                     MKALDPILNLKDKKPAPVVLPMASRTAAAVTKIAVPVSDGLLCAHFGHCEVFALLDVD
                     MDGKKILRREDVTPPPHEPGLLPKWLHEKEARIIIAGGMGSRAQGLFTENGIQVVCGA
                     PADTPEKVVTAYMNGTLATGPNVCDH"
     gene            complement(54830..55312)
                     /gene="tsaA"
                     /locus_tag="PLW16_01575"
     CDS             complement(54830..55312)
                     /gene="tsaA"
                     /locus_tag="PLW16_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPB69624.1"
                     /translation="MSGAIRFTPIGLLRSGHTLAEETPIQPVYAQGAWGRAEVFPEYA
                     EGLQDIESFSHVFLIYHLHLAAPAGLIVKPFLQDRERGVFATCHPGRPNAIGMSLVAL
                     LRREGHVLHLDGADFIDGTPLLDIKPYAARFHRIDRPRSGWHEEVDEQEAVRRGRRAP
                     "
     gene            complement(55309..55671)
                     /locus_tag="PLW16_01580"
     CDS             complement(55309..55671)
                     /locus_tag="PLW16_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB69625.1"
                     /translation="MKIVFTTSGTDLEAPLDRRFGRAPRFLVYDLEGRTFEIVENKQN
                     LNAAQGAGIQSAEAVARTGAKAVVTGHCGPKAFRVLSAAGVVILTSDAPTVAAALDQY
                     RSGVLTEARAADVEGHWA"
     gene            complement(56685..58043)
                     /locus_tag="PLW16_01585"
     CDS             complement(56685..58043)
                     /locus_tag="PLW16_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB69626.1"
                     /translation="MKKHPSRNKLAVDPVNGQDVILDSIADGVFTVDSDWRITSFNRA
                     AEKITGVPRDEAIGQQCKDVLKANICEKDCCLRKTMKSGTPIINRTVYIIDSRGRRRP
                     VSISTALLKGTAGAVLGAVETFRDISVEEDLRKTIEGKYAFEDIVSKNHRMLQLFDIL
                     ADVADSGSTVLIEGESGTGKELFARAVHNLSPRKKQAFVAVNCGALPDTLLESELFGY
                     KAGAFTDAKRDKAGRFRLAEKGTLFLDEIGDITPAMQVRLLRVLQEKTYEPLGSTESV
                     RHDVRIIAATNKNLEERVRQGLFREDLFYRINVFKITLPPLRERMEDVPLLADHFIRR
                     FNVLQQKEIEGLSEEALACLMAYDYPGNIRELGNIIERAFILCKAGRIEKIHLPESLF
                     KMGGGDPRPEPLSLREMEAIHLTNALRANDWNRLRTARQLGIHKTTLYRKIKALRIVT
                     PE"
     gene            58342..59871
                     /locus_tag="PLW16_01590"
     CDS             58342..59871
                     /locus_tag="PLW16_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPB69627.1"
                     /translation="MTAPLIIAMAQGKEVVLLPNMTNRHGLIAGATGTGKTVTLQKMA
                     ESFASIGVPVFVADIKGDLSGIGAAGQASEKMLKRLEAIGIKEFVPRANTAVFWDVYG
                     EAGHPVRATVSDMGPLLLARLLNLNETQSGVLTLVFKIADDNGLLLLDMKDLRAMIQF
                     VGDNAKQFTTAYGNISAASVGAIQRGLMTIEQQGGDKFFGEPMLDIADLMQTDGEGRG
                     VINVLAADKLYNNPRLYATFLLWMLAELFEQLPEVGDVEKPKLVFFFDEAHLLFNDAP
                     TALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPETVLGQLGNRVQHALRAFTPRDQKAV
                     RTAAETLRPNPAFDAVTAITELGVGEALISFLDEKGRPAPVERANVIAPGSRLGVLDT
                     AERSAAIRNSILYGHYEKILDRESAYEKLRASVQGAATPAQPPVTAGDPEAAAPGGGG
                     VLGGGIGDILFGRTGPRGGKTDGLVQTAAKSVARTIGSSVGREIVRGVLGSLLGGGRR
                     R"
     gene            59960..60604
                     /locus_tag="PLW16_01595"
     CDS             59960..60604
                     /locus_tag="PLW16_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPB69628.1"
                     /translation="MLTVLFTIFASSFVVALSGALMPGPLLTATISESSRYGFITGPL
                     MIAGHALLELALVGAILLGLAPFFQRPEVFVATALSGSVILAWMAFGMFRSLPALTLS
                     RKPDPRRRNHPLISGILMSVANPYWIIWWATIGLGYILYSWRFGLRGVVFFFAGHILA
                     DLAWYSLVSATVAGGRQFLTDRLYRGLIAACAAFLVVFAGYFAFAGLGKLNAIL"
     gene            complement(60648..61970)
                     /gene="pcnB"
                     /locus_tag="PLW16_01600"
     CDS             complement(60648..61970)
                     /gene="pcnB"
                     /locus_tag="PLW16_01600"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPB69629.1"
                     /translation="MPPRIVSRKEHRISRRQISPNALRTLYRLRDNGFVAYLVGGCVR
                     DLLLGRTPKDFDIATDAAPGQLKRLFRNCRLVGRRFRLAHLHFQDEIIEVSTFRSAAP
                     VDEGEAGKTEEDHRPPRHLKDEDGMVLRDNVFGTPEEDALRRDFTINALAYNIADFSV
                     IDHSTGLVDLEQRLIRPIGDPYVRFTEDPVRMLRAVRFAASHGFVIEPATWDVLCELS
                     PTITRASSARLYEEVLKLFLLGSAGPVFPLLEESGLGPALFPGLGGGGDENGRRSIVQ
                     ANLAGLDRLCRNGTPPNAPLCFAALFGPDLEEAALARHRDGVPRQQALDAACAVFFEA
                     LCRTVSVPGRIGSQLRAILALQPLLQRQPPRRPARMVHRPEFADGLGYLRLAAETSAE
                     RRPVVAWWDAFLSGDAPPTGKEAPVAETPAKQRRHRRRRPRPATVAAS"
     gene            62178..63074
                     /locus_tag="PLW16_01605"
     CDS             62178..63074
                     /locus_tag="PLW16_01605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB69630.1"
                     /translation="MNPFQLHACLAEAFRNVERHRDAADLIREYSVNRRNIREVALDA
                     VDLGDCRQVLDLGSGFGPFIDVLKGRLAPGTRVTGVDVFGDYGAPFLSACRDAGFQGN
                     FLKEGASVIETFPERAFDLIVCSYSLYFFPDRVPDISRTLSDVGTFIAITHDRNNMRE
                     LISTVKAILAELGHRPDDPLPVENILGRFTAENGRALLQPWFGEIRAIDYPNALVFEA
                     ESQPQLLRYFRFKSPFFLHGTAFTTETVARHLEARLRPRDRFVLSKNDRIFLCSSPRP
                     GNASRPDGGNTRNPLGAIQAPV"
     gene            63071..64081
                     /locus_tag="PLW16_01610"
     CDS             63071..64081
                     /locus_tag="PLW16_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB69631.1"
                     /translation="MTKTSRNYCPHCGKAMGRAREEGVLRDFCATCMRFFYDNPLPVV
                     TALVVTDRRLLLVRRGNRPYRGRWCLPIGFAEVGESIEEATLRELREEAGIEGRVTGL
                     VDVDSVRSGYYGDLIVVTFEVEARGGTPAPGSDTTQARYFPLNALPKLAFSSNRRAVE
                     TYIRQMADTWAIADSFSRAMGNEAGRERTDLLSDALLHVIEWNAEQIVHLWIADVTTN
                     RSTPAYHAVDPARLFRRAHRIVSQFGRWLGGGYDDGDIMKFYMQLGRERREEGFPLSE
                     VLSALSLIRKHIWTFALSRGVWNRTLAIYMALELDRRIVIFFDKATFHASRGYEGATA
                     ST"
     gene            complement(64157..65359)
                     /locus_tag="PLW16_01615"
     CDS             complement(64157..65359)
                     /locus_tag="PLW16_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB69632.1"
                     /translation="MKKLRTLVGIVMICFVFGALGGGIARAEVGVTKDTILLGTFQDL
                     SGPAAYLGRLATGVLQVWMKDVNDRGGIHGRKLKLVVEDNKYDPVLTKTAFTKLVDQH
                     KVFMIVSVYGSTPCTAILPDIQAKKIPVYTTAAAVQTMFDPPQRYLFWYACNGQDQGI
                     LMADYVFKDLKAKNPKIGLCYQDDEWGKDTLDGMEMAAKKYGFKVVEAPYKRGTKNLN
                     AQAMKLKAEGVTHCLFVGYAPVYAALLMEANKIGWKPVFFGDYVSVDPKTFMAGALAN
                     GHYHIGNLGLRHEGGPGWKKIEKGFTEAGLESMLNEPLIPILWNPLLLLTKAMEDCGK
                     NLTRERLIDRVEKIRNFDTGGLGKIQFGKNVRKGAHYYRILQADASKKIFVPVTGWRQ
                     PSLVWGKR"
     gene            complement(65582..66628)
                     /locus_tag="PLW16_01620"
     CDS             complement(65582..66628)
                     /locus_tag="PLW16_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB69633.1"
                     /translation="MAVTRFTIRSYPQDLRIFPSPAARISLVLLGAFLLTFPLFMGSY
                     GVFVACICGVSIISALGLNLLTGCTGLLSMGHAAFMGIGAYTAAILAVRTGLPLIATL
                     PLAGLAAALAGLIVGIPALRLKGLYLVVTTLAFQFIVEHVIYHWESLTQSDKGIALPT
                     PDLFGFPLNSYQSYYYVVLVLAVAAAVFTKNLLMSRTGRAFVAIRDRDIAAEIIGIHM
                     AKYKIMSFAISSFMAGVAGALYAYLLHLIGPDHFTFNQSILYIAMIIVGGMGTVLGSV
                     LGAIFMVLLPEAINAVSGPLVSAYPDLAPRIGGLSVMVYGLIIIFFLLFEPLGLFGIW
                     IRVKRYWKTWPYTY"
     gene            complement(66661..67551)
                     /locus_tag="PLW16_01625"
     CDS             complement(66661..67551)
                     /locus_tag="PLW16_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB69634.1"
                     /translation="MASNILIQLTITGLAWGSVYACIALGFVLIFKATDIFNFAQPEL
                     MMLGAYLTFAFITVLKLPFIGAFLAALVLMALITALLEMLVVRPLVGEPVLSVIMVTL
                     GLANVIRGMTGLVWGYEEYNFPAPFPKDPLTIFGVAINQAEIYTIVATMILLVGFFLF
                     FKYSQSGISMRATAEDTSAAFLMGINVKGVFTASWVIASLVATVAGVFMASLTYLDPI
                     MSHTGLKALPALILGGLDSVHGAILGGLIIGVAENLAGFYLADYLGSGINEITAYVLA
                     LIIMMIRPYGLFGTKEIERV"
     gene            complement(67596..69398)
                     /locus_tag="PLW16_01630"
     CDS             complement(67596..69398)
                     /locus_tag="PLW16_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69635.1"
                     /translation="MREDTVPHIFLARVRKYGDRVALREKEFGIWQEISWNDYSRHVR
                     HFCLGLMDLGLMPGDHVSIFGENLPEWLYADLATQSARGVAVGVYPTNPSREAQYVIG
                     HSESVFVVCDDQEQVDKVLEVREALPFMRKIIVIDMKGLRRGTDPMIVSFEEVERIGG
                     RIEAADPERYWRTVDQVRPEDVAIMVYTSGTTGPPKGAMLSHDNVAQFLKAQSQTLPQ
                     YDTDEIVSYLPLCHVAERMMSVFLPIYSGATVNFAESVDTVTQDMREIFPTFYLAVPR
                     IWEKMMAGIVIKMKDSTWAKATAYRLFMPVGREMALARLSRGPIPFSLRLSHGLGERL
                     LFRHLRKELGLLRARLALSGAAPISPEVLLFFHSLGVPVLEGWGMTEETGIGTANLPG
                     DVKLGTVGKPIRGVEIRIAEDGEILLKCNHVFVGYWKDPEATARTVVDGWLHTGDVGE
                     LDPEGRLKITDRKKEIIITSGGKNIAPSEIENRLKCSPYINEAIVIGDAKPYLTALIQ
                     IEYDNVAKWAQDRGLAYTTFKSLAQLQEVRELVQQEVHEANRDFSQVETIKKFRLLEK
                     ELDHDDNELTATLKLRRKTLYEKLGEIIEEMYRN"
     gene            complement(69395..70186)
                     /locus_tag="PLW16_01635"
     CDS             complement(69395..70186)
                     /locus_tag="PLW16_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69636.1"
                     /translation="MGFLEIREISLHFKGIVALDRVSLNVEEGELVAVIGPNGAGKTC
                     LLNCISGFYRPDGGNILFADRDLAHWRPDQIAKLGISRTFQNVELFARMNVIDNMMLG
                     RHHHFRHGFWHNAVFGYRAAEEAVHRRKVEEIIDFLEMERWRKHLVSSLPYGVQKRVE
                     LGRALVQEPRLLLLDEPMAGMNQEETEDIARFILDIHEELGTTIVMIEHDMDVVMDIS
                     KRICVLDFGRKIAEGTTGEVQRNPEVQKAYLGDDATTLLKKEGAG"
     gene            complement(70196..70999)
                     /locus_tag="PLW16_01640"
     CDS             complement(70196..70999)
                     /locus_tag="PLW16_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69637.1"
                     /translation="MGSLRLNNLEVIYNDIILVLRGISLEVPEGAIVAFLGSNGAGKS
                     TTLKAISGLLDYEDGEIKKGVIEFDGHPIHKMDPQEIVQMGITQVPEGRRIFAELTVD
                     ENIRVGAHTRRDGKAEIARDYERVLGYFPPLRQRLKIQAGFLSGGEQQMLAIARALMA
                     RPSLIMLDEPSLGLAPLLVTEIFDIIRRINREERVSILLVEQNAFMALAVAEYGYIME
                     NGKIVLDGPSGKLMENEDVKEFYLGMKDEKERRSFAEVKHYKRRKRWLS"
     gene            complement(71510..71995)
                     /locus_tag="PLW16_01645"
     CDS             complement(71510..71995)
                     /locus_tag="PLW16_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB69638.1"
                     /translation="MGEQDKGELRDALQGYKAGITPGVKATLAWNRELVFAGTTPQGY
                     EIEFDAESQWGCNPTEALLLSLAGCMAIDIVSILKKMRVALTAFRVDVTGQRNPEPPQ
                     YFRSVEMALHMAGQNLDARRVERAIALSRQTYCSVYNSLRPDLELTVRYVFEETAAAP
                     S"
     gene            72164..73018
                     /locus_tag="PLW16_01650"
     CDS             72164..73018
                     /locus_tag="PLW16_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB69639.1"
                     /translation="MSQEKNVYQFRGGSKYVLDEELARIVNVSMALEMPLLLKGEPGT
                     GKTMLAHAIAESLKMPLIVLNVKSSMKLIDALYQYDTLTRLNDSRFGDSKRDVSNIED
                     YIKMGKIGQAFTAETRTILLIDEIDKADTDFQDDMLDVLDQMQFDIIEIDKTMTARHR
                     PVIVITSNAKKDLSDPFLGRCNFHHIAFPDPEMMRRIVNVHFPNLSDDMLDACIAAFY
                     RIREFKGIEKKPATRELINWIRALKTDPDFRVRALEKGETPFLGILFKKSNDLQMAAQ
                     QAARSSLR"
     gene            73028..74221
                     /locus_tag="PLW16_01655"
     CDS             73028..74221
                     /locus_tag="PLW16_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69640.1"
                     /translation="MFVDFFYTLRDQGIPVTPTSFLRLHKACSMGLVNSLEDFYTTAR
                     CVLVKSERYFDLYDQIFSHYFQGMTLETWDDQEITAAARALLDEWLQNPKELADALGL
                     DAEKLKAMTPEELIKYFFDRLKEQKEAHHGGNRWIGTGGTSPVGHSGHHPGGMRVGGR
                     SGNKSAIKVALERRYKDYSQEGPITESQLSEALKRLRRMIPHGPKDVVNVEKTIYETM
                     RNGGEIDIVFDRSLRDRLKVILMIDNGGWSMDPYIETVQTLFNYARSQFKDLKIYYFH
                     NTVYSKVWADPQRRLKPEPLAEFVRRDPETRLFIVGDASMAPYELMHVNGSIYVDDRQ
                     SLASMDRLRVLQKTFRHSVWINPQPEEEWSYTWTVNAIAEVFPMYPLTLDGLDRAVQF
                     LVKKN"
     gene            74278..74847
                     /locus_tag="PLW16_01660"
     CDS             74278..74847
                     /locus_tag="PLW16_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB69641.1"
                     /translation="MKTKMGIGLVVLTALLFSLCPELPAVPPPAVGGTLPNVELPGPK
                     DGSDRRYLGLSGSFKIPEIKAQAVIVEIFSMYCPYCQKEAPVVNQLFARIEGNPALKG
                     KIKMLGIGMGNSPFEVGVFKKKYKVVFPLFPDPDFVIHKRLGEVRTPYFIVIKIHPDG
                     SHRVIYSKLGAFEGVDAFLAAIVRDAGIK"
     gene            74888..75493
                     /locus_tag="PLW16_01665"
     CDS             74888..75493
                     /locus_tag="PLW16_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPB69642.1"
                     /translation="MKKIGPIGAVCFLGAFLLLAAGVPVDAASEAFKDNIYDTGPLKP
                     RDSVLKVKVGRFAPDFTLRAVAGGPVSLRQFRGKKNVVLSFVPAAWTAVCSDQWPGYN
                     IVKELFDEHDAVLLGITADNIPTLYSWTNQMGTLWFPVLSDFWPHGKTAARYGVLRSD
                     GTTERALFVIDKRGVIRYIDVHDINQRPSLEVLMGELEKLP"
     gene            complement(75542..76678)
                     /locus_tag="PLW16_01670"
     CDS             complement(75542..76678)
                     /locus_tag="PLW16_01670"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPB69643.1"
                     /translation="MAAALLIIFYLLFPVVAIWLAGRYALFNKLGAVVICYIAGIILG
                     NIGMMPAGIAALQNPLMLVTISLALPLMFFSIDVRRWSRLAGKSLLSFMLQILSILIV
                     ATLGFFVFRGWVGEETWKLAGMFVGVYTGGTVNLGAIATALRTDQTLYLAAHTSDVIV
                     SSVYLLILMTVGQRLFLRFLPAFRPAETSAPETEFEFDSYAGIFAKETFLPLLGALGL
                     AVLIFVAGGLSFLIFTEDVAFVTAILTISTLGIVFSFVPTIRKIAMTYQAGNYFILIF
                     CLVVSSMADFNRLVSTAPIMIAYVTFTVLACLVLHTALAALFRIDADTVIITSVAGIC
                     SPPLVPMVATALKNKEIVLSGVITGIIGWVVGTYLGITLSYLLR"
     gene            complement(76818..77702)
                     /locus_tag="PLW16_01675"
     CDS             complement(76818..77702)
                     /locus_tag="PLW16_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69644.1"
                     /translation="MHLMSLKTRPVAAMVLLIMVTLFVPPSPAGGEPRVTSDKDRYRS
                     GEKIQVHFSGAPGDGGDWICIVPAGSPDTDAGDYRYMPEGTSQGVLTFDAPPPGKYEV
                     RAYYDYRRNGYVVSARYGFAVESGPVLLTRPKPVAGPETTAPVESQPVKAPPLIPSQI
                     SVAVFHFTSSSMDASQYAVKVLGTLTNAPKMQASFDLLDRKDLETFLVVNDLHQTDRI
                     EDVIHIGTRLGLNIVITGSIEKRGTLIIINYKVISIDHRRVIFTDRSLSLGEASLVSD
                     VMKMSDAMIEAILRSSSR"
     gene            complement(77807..78451)
                     /locus_tag="PLW16_01680"
     CDS             complement(77807..78451)
                     /locus_tag="PLW16_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB69645.1"
                     /translation="METSVAEARRIGLLADSHGDLGRTEQAADALLAAGAERLIHLGD
                     FCDTVQESSWMRVMQALRRYRMMTVKGNNDFLVETILKERPPGPDPAEVQLRAFLKSL
                     PMTLVADGLCFAHSLPYDSLRSFYEPVDDGTTAKAIPVFQSTAHRILFCGHAHTPVLF
                     RWRAGRVSREAIPPDLPVPMRADERYIAVVGAVEDGECALYDGGEGTYRRIRLD"
     gene            78630..79715
                     /locus_tag="PLW16_01685"
     CDS             78630..79715
                     /locus_tag="PLW16_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69646.1"
                     /translation="MKRPRALVNGATVTAALLLILALAACAPYKAWLKGQAENHATRH
                     IAFWGEAWQSAPLSARLAPAPPALIEKIRLENRLYGFPERPEPVTPEPVFTEAVRRIG
                     DLLPERVRSLAEQRIIGIYLVNGLGGTGYAEAILNAEGKERYAVIVLDRDVLLTRRAN
                     AWASWKENSFFRPATGMETALELILETDEEDSVVNAIVFILLHEMGHALGMASGVHPS
                     WNGPAEVSDAYPFTALSWRRQGSDVVSRADAAFPRRSALKPYAFAGARLSLDEAPEIY
                     RALQAHSDFPSAQAAVTLWEDFAESFATYLHVVRQDKSYEIRIRRPDRPEQAFPSCWR
                     EARCDNKKAFMRRWFENPVSPALPAPP"
     gene            complement(79840..81015)
                     /locus_tag="PLW16_01690"
     CDS             complement(79840..81015)
                     /locus_tag="PLW16_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB69647.1"
                     /translation="MTMTDEHNGLARAERLYRDRALRARELKEGGARLMGYLCLYPPV
                     ELMTALGFVPFRVFGDRRQPVTEGNRVLMPAVCPFLRSLIDLGMKGQYAFLDGLVGAH
                     ICDVGSMSVHLWRDYVTAPAFVHFLDVPHTVHAPALDFFRTQIEILLKDLEGLSGRRL
                     DGTALRAAITAHNRQRALLRDLYDLRRTVPPRLTGVEAQRAALAVTGLPVEEGNDLLA
                     AVIAEVRQRPLPAPSGAPRLLVWGSVIDDTSLFELVAAAGAEVVTDDICVGTRAFSTD
                     VETEGDPLTALARHYLADIRCPRTYRQADDAGGRPYREDLEARFGYLAPLIRDWRVDG
                     VILQSVMYCDTHGYEIPAVRDYLNGLGVRNFYLEHDYSDGSRQQVRTRVEAFVEMLR"
     gene            complement(81114..82472)
                     /locus_tag="PLW16_01695"
     CDS             complement(81114..82472)
                     /locus_tag="PLW16_01695"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB69648.1"
                     /translation="MGEPELKPPKPGKAINRLKSMYPLRNVVNEAYLTTLMASQAGNP
                     TAWSMLNWWEGDILLKAMDVEVVYPENYATIAASMGQAEGYLALADAEGFPAHLCGYA
                     RTTLGYTKRMMVDEAGRVPPDAPLGGMARPTLLVGAGTACDARYKWFQALGRYFDCPQ
                     WVLELPHLGVKEFQEDGAEEAAVRFVVAELRAFVAFLERLLGRKMDWAKLEAVVDLAL
                     KIQRVFHEVNELRKHRPCPMHSGDFWSAMPGCLFLTGHLPKVLELYEDMHREVAERVA
                     QGVAGIAVPEKYRLLFAELPPWHSLKFFENLAERGWNFVVESYAYHPVVPMDLSAVPD
                     PLERIARICMRFFHLRHRQAFSDGVSYAFVAPYLDSARTYRCDGAFLHPLLTCRTASA
                     HLQATQDVLIRKLRVPSLLAEGDIVDIRLFNPERTLIQAEAFEATMDHYREERRQAGS
                     DW"
BASE COUNT        15195 a        27132 c        25442 g        14948 t
ORIGIN      
        1 acggcccgga acgcgtcgtc gtcgtaggac aggggcgccc cgaagatggc catgatgctg
       61 tcgccgatgt gcttgtccag ggtgccgttg tattcgaaga cgatccggtc gagcggccgg
      121 aaatagtacg tgttcagaag atcggcgatg tcctggggcg acatccgggc cgtcatgtcc
      181 gcaaaccccc ggatgtccgt gaacagcacg ctgacggtcc gtcgcttcgg gagaagtttt
      241 tcgccggtgc ggtgcatctc ctggaggacc tgttccgaga cgaactgttt caggcgcttc
      301 ttggtgatga attcgcggat cttggcccgg atttccttgt tctcgaaagg cttcgtcaga
      361 aacccgtcga tcccctggtt gaccgtgtcg atggcgcggt ccagcgtcgc atagccggtc
      421 aggatcaccc ggatgatatc cggattgatc cggccgatct ccatcagcgt ctccaggccg
      481 tccagcccgg gcatcttgaa gtccgagatc accacctcga tgtcttcggg aaatttccgg
      541 acgaggtcga tcccgtccgc cccccgctcg gccagcagga cttcataccc gtcccgttcg
      601 aggaccttct gcagggatat ccggacgcct tcttcgtcgt ccacgatcag aatcttcacg
      661 gcgcgccgca cctcctgcat ggcagggtga tggtgaacgt ggcccccttc cccgcctcgc
      721 tgttcacgtg gatctcgccc ccgtgcttgt ggacgatttc atgggagata tagagcccca
      781 gccccgttcc cttcccgacc tccttggtcg tgaagaaggg cttgaagatg tccttgagcg
      841 tctggggcgg gatgccgacg cccgtgtccc ggcacgtgac gacgatgctt tccgtcttcg
      901 aatcgcgcgc ggtggagagc gtgagggttc cccggccctc cggcagcgcc tgcagggcat
      961 tgttgatgat attgatgagc acctggccga ggttggagaa attgccctga agggccggca
     1021 ggtccggatc gtactgcttt tcgataacga cgtccatcgt tttgtactgg ttgtagagaa
     1081 cctggagcgc ctcgtcgatg acggcattca tgttgacgtc ctcgagatag gtctgcgtct
     1141 ggcgggacag gtccaggatg ctcttgacga tccgctgggt cttgttcagc tccttgaggg
     1201 agtaggccag atccttccgc aattcctcaa agatcgggcg tttctcctcc tgctcctcga
     1261 gcagctcgat atcggtctgg atgaggctcg atgcgctggc cagcgggttg ttcagctcgt
     1321 gcgcgatccc ggcgacgagc tggccgatgg cggccaggct ttccgactgg atcaactgct
     1381 cctgcgtcct ttccttttcc gcgagcgccg ccttgcgccc gttgatgagg gactgggtca
     1441 tcaccccgta cacgatgacc aggcagacga tgaccacacg cgtgaagacc tgctcggacg
     1501 ccatggagtc gaacacccag tagatgagga ccatggcgat ggcgatgagg atgagnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatg gagtcgaaca cccagtagat
     1681 gaggaccatg gcgatggcga tgaggatgag accgttggct tcgatctgct ggcggagcgt
     1741 gggcttgttt tgcatacaat tcctgtcggg atctctcgcc gggaggggat ggcgggcagg
     1801 catcctcccg cgaagtctca gacgccgtaa agcacgcgca tcctaatgaa aaaaagggga
     1861 tgtgtcaaga aacacttgcg atttgcagcc gtggttcgac gggctcacca cgacatttgg
     1921 ggggcgccat gacaaccaag cttgtcgccc tgagcttgtc gaagggcctc atgaaaagac
     1981 tgcggaggcg tcagggcttg gcggcggcgg tccggtcgtc gcggaggcgg aaggggttga
     2041 agtcggttcc gatatcgtaa acgtccaccc cctccgcggc cttcagccgg cccacgatcg
     2101 cataggtcaa cggggtcatg aggacctcga cgccgacctt gaagaggtag ttcgtgacga
     2161 cgagggtgac gaacagggcc gggggaaaga cgccgaacag cgtggccgcg ccgacgaagg
     2221 ccagcgtgtc gatcccctcg ccgatgaggg tgctgcccac ggtgcgcgtc cagagccagc
     2281 gcccccgggt catcagcttc atccgggcca ggacgaagga attggaaaat tcgcccagcc
     2341 agtagcccag caggctggcc acgacgatcc cgccggaggt catcccgccg aacacggcgt
     2401 cgaaggcgcg ctggccgacc gactgctccc acagggcctc cccgggcaga atccggaccc
     2461 cgtacaggag gaggctgcag acggccagac cgaagaaccc catccagatg acccgccggg
     2521 aacgcttgaa tccgtagact tccgtcagga tgtcgccgaa gatgtagccg accgggaaca
     2581 gcagcgtgcc ggcgtcgaag gccagcggga ccccccccag cgagacgccc cagtccacga
     2641 tcttcgccga ggacaggatg ttgctcatga cgagcacgat cacgaacagg accgtgatca
     2701 gatcgacata ccggtaggcc ttccgttcca ttttttcctc cgagcgaaag gccctatcac
     2761 ggaacccgcc gccgggtcaa gcgagcgcgc cgccgtgctt gaattcataa aaccgatatg
     2821 ctatgcagag ctcgccttcg agacaggccc tgtttcttgg ggaagcggcg ccgtacggga
     2881 ctccgggaac gggcgccatc cgacacggga ggacggaacg ttgccaagcg ggaaaatctt
     2941 cttcgtcgtc aatccgcacg ccgccaacgg tgaaacgggc cggctgtggc cgctgatcca
     3001 ggccaaggcc cgggagaggc tggggccgtt tcgcgcccaa ctgaccgccg ggaccgacga
     3061 tgccacccgg atcacccgtc aggcgctcct ggacggcgcg gagctgatcg tctgcatcgg
     3121 cggggacggc accctgaacg aggtcgtcaa cgggttcatg gacgagaagg ggccgatccg
     3181 gccggaggcg gtgctgggcc tcgtccccaa cgggacgggc tgcgacttcg tgcgcacggt
     3241 ccccattccc gccgacctgg acagcgcgct caacacgatc cgcgccgggc atacccgggc
     3301 cctcgacctg ggccgcctcc gcttcgcgga ccaccggggc gggaccggcc tccgctattt
     3361 ccacaacatc gcgagcttcg gcatgggcgg cgaggtcgtg gatcgcgtca accgcacctc
     3421 caaggccctg ggcccgttca tctccttcat gtggggcacc ctcgtttccc tgatgcatta
     3481 caacgccaag cggatccgga tcaaggtgga cgactacttc gaccaagagg tcgccatctg
     3541 gcatgtcgcc gtgggaaacg gccgctacca cggcggcggc atgctcgtcg ccccggacgc
     3601 ccggatggac gacgggctct tccacgtcac gatcgtcagc gacatgatcc tgccccgcgt
     3661 cttcatccac ctgcccaagc tctacaacgg caagatcaag actatccggc aggtgtcgac
     3721 cctcgtcggg aagacggtca gcgccacctc ctccgaccgc gtcctcctgg acgtcgacgg
     3781 cgaacagccg gggtgtctgc cggccacgat cgaaatcgta ccggccgcca tccggatgat
     3841 cacccagcgg ggctgacggg cgccgcccgg gctgaggtca gggcttctac gctgtgaccc
     3901 gctcgggaca aggcctccgg gaggaactcg caggccacgg catcgtcgtc aatcgccaga
     3961 atgcggacct tcccggacgc cttcatccgg atctcccttt tctcacgtcc agcccttgcg
     4021 gcccatgaaa tcggccaggt ccgccacccg gcaggaatac ccccattcgt tgtcgtacca
     4081 ggcgacgacc tttgccatgt tgtccctgag cacctgcgtg aactccagat cgatgatgga
     4141 cgagtggacg ttccccttga aatccgagga gacgagcggg gcctcctcgc aggccatgat
     4201 gcccttgagc gacttcttcg ccgcgttcgc cagaaggcgg cgaagcgcct cggtcgtcgt
     4261 cggcgtcttc agttcggcca cgaaatcgac gatggagacc gtcggggtcg gcacgcgaag
     4321 ggaatatccg tcgaagcgcc cggccaggtc ggggagcacg agggcgagcg cccgggcggc
     4381 gccggtcgtc gtcgggatga tgttcatcgc cgccgcgcgc gcccggcgga ggtccttgtg
     4441 gggcaggtcg aggatccgct ggtcgttcgt gtaggagtgg accgtcgtca tcatcccctt
     4501 ctcgatgccc agggcctcgt ggacgatctt caccggcggg gccaggcagt tggtggtgca
     4561 ggaggcgttg gagacgatgt ggtgtttctt cgggttgtac gccttgtggt tgacccccat
     4621 gacgaccgtc aggtcctcct ccttggcggg cgccgagatg atcaccttct tcgcgccggc
     4681 ctcgaggtgg gccgccgcct tggggccggt gacgaaaagg cccgtgctct cgatcacgat
     4741 gtccaccccc tccgcgtccc aagggatggc ggccggatcg cgcaaggcga aattccggat
     4801 cgttttgccg tcgatgacga aggcgtcctt ccggaccttg acctcgccgg ggtaggcgcc
     4861 gtagctcgtg tcgaatttga agaggtgggc gttcgtccgc acatcgaaga gatcgttgac
     4921 cgccgccacc tccagcgtcc cgggataccg cgtcagaacg gccttcagca cctgccgccc
     4981 gattcgtccg aatccgttaa tgccaaggcg aatcatgggg acctcctttt caattgtcgt
     5041 cgatgcggtt tttgtgcgcc gccatgagat cgcagcggta ctggagggtg cggtgcatcc
     5101 gggcgttgtc gagggcgatg gcgctcaggt tcgccacggc ctccaggaag cgcacctccc
     5161 ggtcgttgaa ctcccggacc cggtccgtgt aagcgcgcag cacgcccacg gccttcttct
     5221 ccagcaggag cggcaccacc agaacggact tgatcccctc cgagcgggcc ttggcgccgt
     5281 actgaaaatc ccggttcgtc tgggcgtcct tgagccagat ggtcttgcct ttcagggcct
     5341 tttgatccag gccgctttcc tccaccatga tcggcccctt gtggagatag gtctcggaaa
     5401 gaccgaaggc ggcccccagc tccagcttct tctcccgggc atccaggagc cgcaggctgc
     5461 aggccttgac ccgcatggtt tccgtgacgc agcgcacgat ctcgtcgaga acccgggcgg
     5521 gctccagaga ggcattgatg accttggcca cgtcatacag ggcagtgaaa taatccattt
     5581 ccttcccctt catcgcctca cctcctgaac gcgtatttgt ctttttcatt aatgtttccc
     5641 gaacgccaac gcaagcactt attgtccggc cgttcttcct gtcatttcga ccgtccttcc
     5701 tgtcatctcg accgaaggga gaaatctttt agaagcggcc gtcgtaaaga tttctcgtcg
     5761 cccacgctcc ttctaaatga cacggggggt gaaagatttc tcgtcgctcg cgctcctcga
     5821 aatgacgtgg tgcgaaggcc tctaaatagg caagggtctc gaaataggaa ccccttttcg
     5881 gaccccgaaa aaacacgatc gcgctcgcga tctttgggcg ccgtcgaagg gccggccgga
     5941 ctcagcaggg cggctggaag gggggaagaa cggtgggcct gggcagccgg cggggcagct
     6001 cgccccggat ctcgatgacc ggaccgccgg aaatctcctg ctgggttccg gaatcgaatc
     6061 cgtgcttttc gtcgcctacg gcccagtcgt agagggcgcg ggaatcgaac agcaccgggt
     6121 cctccggtgt cccgtaggtc ctcagctgca tgaactcgtc gtagacgccg acgcacccgt
     6181 gcgaggcggg ccggcccggc atatcccggg cgtggatcca gaaggcggcg ccgtccggcc
     6241 ccacgtaaaa cagcagggcg taatccatcg ggtactggcc ctcccccttt tccgtcttgt
     6301 acatggaaga ggtgtgcgtc cggtcccggg cggtcacctg gaagaaaccg agaggcgtgg
     6361 ggtgatcggc cgtccccgtg gccgccggca tggaaagcct gaggttgccg tattcgtaag
     6421 cccccagcca ctgttcggtc aggtcgagca ggaggtattt cgggtagggg cccgccggct
     6481 cgtaacgcat cgggaggggg gtgtacgccc gcgccgcgat catgtccacg ggcaccttga
     6541 tcgtcatgcc cgggtaggca tggcggcggt cgagacggtt gaagcgggcc acggtcaccc
     6601 agtctttccc gaagagccgc tccaggctgt cctggggccg caggaagtgg ggccgccagg
     6661 ggacgcggat ctgactggga tatatgacgc ggctcaggtc ctccttcgcc ttgtccaagg
     6721 gcgaggcgga cggttcggac gtttggccgg gcagggacat ggcaccggcc ccgatgatca
     6781 ccagggcgat gacgacccga accgcccggt ggagggtttc gattccgaat gccggcatcg
     6841 gcttcgcggc cttccggaaa ttctcgctgt ctcccactgc tcgcgccccg atcggcgtga
     6901 cgggccccac ggcccgccgt acgcggcgtc ctctatcacg ttttgccgcc cccggtaaag
     6961 ggcaatcacg gacgcggccg gggcccgttt ccgcgtatcg tcaccggcga aatcgcccga
     7021 tgcctcccgg gaaggcgcgt ttgatcttga ccttccgcgg tcaaagtcgt agggttttcc
     7081 caggttttcc gaacggaacc gtgtttttga aaaatgccgg catttcgggg aggagcgtga
     7141 tgggtcgcat cgggaaaatc accgtcggtc tggtcgtcgt gctcggggtt atcgtcatcg
     7201 ccggattcta cgttctgccg ccgttgatga agccctgggc cgtcgagaag cttgcgggcg
     7261 tcctcaagcg ggatgtcgcg gtcgaacgca tccggctcaa cccgttccgt ctgtcctgca
     7321 ccgtcgaggg cgtcatgatc cgagagcccg cgcagacgga tcccttcgtc tccttcgacg
     7381 ggctgtacgt caacgtcggc gggctgtcct ccctgttcca ccgcgcactc gtcctggagg
     7441 aggtccgcct gacgcgtccc tacgtgcaca tcgcccggaa accggaccga acctacaatt
     7501 tttcggacct catccccagg gagaaggagc cgcccgaacc ggagaagaaa ccgttccggt
     7561 tctccctcaa caacatcgcc ctggtcgacg gcagcgtcga tttcgacgat cggccgatga
     7621 acacccgcca caccgtgcgc gggatgaatc tcggccttcc cttcctctcc aacacggagg
     7681 tccacgtcga ccatgacgtg gagccccggc tcgaagcgct cgtcaacggc gacaccctcg
     7741 tcctcgccgg aaaatcgaag gtgttccaca aatcgaagga gtcgcacttc acgctgaacg
     7801 tccgggacct cgacctgccc cactacctga attacgtccc cgtgaagctg aacggaacgc
     7861 tcgtgtcggc cgtcttggac acgaacctgg atatcgcctt cgtcatgccg gaagaccaat
     7921 caccggccct tcggctgggt ggggatgtga cgctccgcaa cgtggccttc gacgatccgc
     7981 ggggcgccgc cgtcatccgc ctgccgtcgc tgcgcgtcga cctggccgcc gtggagccgc
     8041 tcgtccccga catccacctg gcgaaggtga tgcttcagtc ccccgagatc gccgtccgga
     8101 gagacgggaa tggccgcatc aatctgctca acctcacccg cacgcctgaa aaaccggcga
     8161 agcgggaggc gccgaaggcg gaggaaaagg cggaacaacc ggccggaaaa ccggccctca
     8221 aactgcggat cgacgaggtg gcggtcgaag cggcgaagct ctccttccgc gacgagacgc
     8281 ccgcccaaac cgccgatatc cgggtggcgc cgctcgacct ccgcgtcgcg aacctgtcca
     8341 ccgagaaggg ggcgaccggc accgtcgatc tcaggctcac ggtcgagcgg cagggaacgg
     8401 tgaccgtcgc cggcccgttc ggcatcgagc ccctccgcgc cgacctggcg ctcgacgtca
     8461 aaaagctcgc catccggacc tttcagccct acttcgccga caagatccgg atccacgtgg
     8521 ccggcggcac cctgtcgctc gcggggaacg tcaacctgtc ccgggagcgc aaccgcgacc
     8581 cccgggcgac ctacgcgggc aagatctacg tctccaacct cgccaccctc gaccagaccc
     8641 acgccaaccg cttcgtgaac tggaagcagc tctatttcga gggcgtccgg gcgggcgtca
     8701 atccgttctt cacgaatatc cgggggatct ccctgaccga ttactacgcc cggatcatca
     8761 tcaatccgga cgggaccctg aacctgacga acatccttgc cggcgaaaag aaggaaaccg
     8821 ccggcggcgc cccggcggaa ccgaagcccg ccccggccgc gcaggcgaag acggcgcccg
     8881 caaaagccgc gatggacatc aagatcggca aggtcacggt ccagggggga accatcgact
     8941 tctccgaccg cttcatccag ccgaactact cggtcaagat gctccgcatg ggcggaagcg
     9001 tcacggggct ctccacggat caagcctccc gggcggcggt cgatctgaag ggcaatctcg
     9061 ggcggggctc gcccgtggag atcgcgggga agatcaaccc cctgatcaag gatccgttcg
     9121 tcgatatgaa gctccacttc aaggacatcg agctgagccc gatgaccccc tattccggaa
     9181 agttcatcgg gcatccgatc gaaaagggca agttgacctt cgacgtcgcc taccggatcg
     9241 accagcggaa gctggacgcc cagaacaagg ttttcttcga ccagctcacc ttcggagacc
     9301 gggtggacag tcccaccgcc atcaaggtcc cggtgaccct ggccgtcgcg ctcctgaagg
     9361 accggaaggg cgagatccac ctcgacatcc ccatttccgg aagcctcgac gacccgaaat
     9421 tccgcgtctg gccggtcatc tggcaggtca tcgtcaacct catcaccaag gcggcgaccg
     9481 ccccctttgc actcctgtcg gccctcatgg gcggcgggga ggagctgagt tttcttgaat
     9541 tcgacgcggg tagcgccagg atcacggaga ctggccggga caagctcaag gcgctggcca
     9601 aggccctcta cgaccggccc ggcctcagga tggacatcga ggggtatgtg gatgtggatc
     9661 aggaccggga ggggctgaaa aaggcggagc tgaaccggaa gctgcgggtc cagaaactga
     9721 atgacctgat cgcgcggggg cagtcggcga cgctgaggga catcgggatc gaacccgggg
     9781 aatacgagaa atacctcgcc ctggcctacc gggtggagac gttccccaaa ccccgcaccg
     9841 ccgtgggcgt gacgaagggc ctcccgcccg cggagatgga gaagctgatc ctgacgaaca
     9901 tagcgatcag cgacggtgac ttgaaggcgc tcgccgcacg gcgggcctcg accgtgaagg
     9961 acgccctcct gggatcgggg gagatcccgc cggagcggat cttcatcgtc gaaccccggt
    10021 ctctggcgcc ccggaagcag gagaagcaaa aggacagccg cgtcgacttc aagcttaagt
    10081 aggaccgccg aactccgcct cgccgaagac ctcggcctgg aaggtcagga actccgtgcc
    10141 ctcccgccac gcgccgggcg agaggcccgc cttcatcgca aggttgtcga gcatctcgtc
    10201 ccgatcccag ccctgctccg tcgccacctg ggggagaaag acggccgaat gccgcccctt
    10261 gcggatcagc acgccgtccc ggccgacgat gatgtccccg ggaccgcgga ccggcgtcat
    10321 cggggtcagg acggagattt cgatttccag gtccttgagt tcctccgccc tcagttcggc
    10381 gaagcggctg tcgtggaagg cggactggac ggccatcgtc ccgacaagct ggcccagcgg
    10441 catgtccccg accattcggc cgatgcaccc gcggagccgg ccgcgctttt tcagggtcac
    10501 gaaaacgccg cgacgaacct cgagccgggg ggaaagcccc cgcgccagag gcaccgtctg
    10561 cgccgtcagc aggcgcgtga tcgtctcccg ggccagggca agcagggcct tcttgtcctc
    10621 catactcagc ccgccggcct cctcccccac cgcctccggt tttgcattca tttcgacgac
    10681 ccctttcgca aaggccacgg cgccgtatcc caccacccgg ttccggtcgc ctacggccac
    10741 ctggccggaa tgggcatagc tgacgacccg ccccgccgcg gcgcccaggg cggtcgccgc
    10801 cgtcatggcc gccaggatgg gaccctcgcc gcaggcgcag gtgtggaggt cgcgaacccg
    10861 gcgggccatc tcggtcctga tgcgctcccg aacgcgggcg gggtccatcc cggcgatggc
    10921 ctccagaacc gccggatcga cccgctcggc atcctccgcc gctgggtagt gggacagatc
    10981 ggaactcgcg acgatcaggg cgcgacggtt acgcagcacc gcgcccaggg cctccccgaa
    11041 ccgccggcag agatcgggtt ccgcctcgcc gaccacggcc ggaacgatcc tcgcctccgg
    11101 gaagaggacc tgcacgaagg ggacctgcac ctcgacggaa tgttcccgct cgtggacggt
    11161 cgcgtcacgg atgccgtccg gatcggccgc gatcagggcg gagaccgccg cttgatcgat
    11221 ggcggcgatc ccgagggggg tccggaaacc cgcgccggga taaaagccga tcttccggta
    11281 attcggccgg gtgtgattcg tccccaggag cacaacgagg tcatacccgc cgcccgccgt
    11341 ctgccggaag gcatcggcgg caacctggcc ggagtagata tatccggcgt gaggcgcgac
    11401 gatggcgacc gcatccttca cctgaaccgg cgcggcgtcg gccaggaact tctcgacggc
    11461 cgtcctcagc acccgcgcat cccccggata gaacttcccg gcgaccacgg gggggcggat
    11521 cccttcctct tccgacgatt gcttcctatt ccacgacatg gcgtcctccg atacaaaaac
    11581 cctcgaccat gatgttccct ctaaaattgg atcccttcat gtgcgcccgg gagatttccc
    11641 gggactcctg ccccgcggcc ccggcgcccg cgaagacgac gctcttctcc gccggcttga
    11701 tcgcgtcgat gtaccgcggc ctcggcaccc gcgcaaacaa ccggtcaggc atgcagcgga
    11761 atcgggattc ccaacgcgcc cggcccttca tcggagttcg cgaagctgtg ttcccagtcc
    11821 ccgcatcgcc cggcgcaggt cggcatagat ctccgccgcc ggtcgcgcgc cggcgcgcac
    11881 cacatcgaag acctgatccg gatacaggcc ccgaacccgg gggaccactt cctcccggac
    11941 gatcccgata aagaccggcg gcttcagcgg tccgcggatg agcaagtcgg ccagccagcg
    12001 gaagccgccg cccgccagtt cgaggggacc gatttcgtcg agaaacacga gcggcgtctt
    12061 gagcgtcacg gcccttccca cggcctgggc gccgaaggcg aatcccgccg cgtcgacacg
    12121 ccagcggccg atcgccgtct cgcccgccgc gggggcgcgt tcagggttgt gcaggtgcgc
    12181 gaagggacgc gcctgaccgc tcagcaggtc gcggatgaca tacgtcggac cggccccgtc
    12241 ccgctcggcc ggcagacaga gaaaccctcc gggcaggagg tcctgcctcc ggaactggtc
    12301 cgccagcccg tcggccaatc cggtccggcc gtcgcgggtg gcgccgctca acaaaacgtt
    12361 cattcgggtt ctcttttctt ccggtccctg ttcatgcttc ccgaacggta gccttcgaga
    12421 tcgagcgaga cgtaacggaa gccgaccccc tgaagggttc ggtggacgtc acgccgcagt
    12481 tcggcatcga ggagtcgttc cagatccgcc ggtgcggcct ctagccgcgc cagatcgccg
    12541 tggctgcgga cgcggaccgc ccggaaaccg agcccggcga gatgacgctc cgccgcctcg
    12601 atccgccgga gacctttggc ggtcacgacc cgaccgtggg gcagccgggt caggagacag
    12661 gcgtagggcg gcttgtccca gaccgtcagc ccccgggccc gcgacatcgc gcgaatgtcc
    12721 gacttggtcc agccctgctc ccgcagggga ctgacaatcc cgagttcccg gagcgcccgc
    12781 atccccggcc ggtcctccgg ggcatcctcc acgttcgtcc cgtccatgac cgtcggaaag
    12841 ccccgggtcc gtgcctccgc gagaatcaga tccatcaggt gatgcttgca gcggtagcag
    12901 cgatcgggcg gattgcggcg gatcgcctcc ggaaacgcca cgtcaagcct cagatgcgcg
    12961 acaccgagcc cccgggcaat ctcgcccgct tcctccagct cccattgcgc catgtacacg
    13021 gtttcggccg taacggccag cacccgatcg cccaaggcct cccgggccgc gagcagcaga
    13081 aacgcgctgt cgaggccccc ggaaaaggcc acggcgcagg ggccccgttt tcgcagacga
    13141 tccgtcaggc gcccgtaccg tttccgcagg gcctccggcg ctctctgacc ggttgtcgca
    13201 cccgcggtca tagacgggaa cacctccgtg gccatctcgg atcactccaa acgatagccc
    13261 tgttcacgga agagccgcag gcacacctgg accacctcgg gatcgtagag gatcccctgc
    13321 tggcgctcga tctcctccag ggcggacacg atgcccaccg ccggacggta gggccggtag
    13381 gaggccatcg cttccaccac gtccgccacc gccaggatct tcgcctccag gagaatgtcg
    13441 tccatggcga gcccttcggg gtagccgctc ccatccatcc gttcatggtg ctgggcaacg
    13501 atgtgtgcca ccggccaggg gaactcgatg tctttcagga tgtcatatcc ggtatcggca
    13561 tgggtttgga gcaggctgag ctcgatgggg gtcagacggg tcggcttgct caggatctcc
    13621 gccggcaggc cgatcttgcc gatgtcgtgg atcatggccg ccatcgccac gccttccacc
    13681 tggtcgggcg gcaggcccat cgcctccgcg atggcacggc atagctgcga gacgcgtctt
    13741 tggtgccccg ccgtgtaggg atccttcatc tccacggcca tggacagggt cgtgatgatc
    13801 gcctcgaccg ccttgcggag gttccagagg gttttttgaa gatgctcctc cgcctccttg
    13861 cgctcggtga tgtccttgat ggtcccctga aatccgaccc gaccgagcgg gtcccgccac
    13921 agcgtggaac tgatgagggt gtggtggatc cggccgtcga gccctcgcag agtaatcggg
    13981 tagtccatga cggatccctg gcgttcgatt gcctcgatgt gccgggcgcg ttcctccgaa
    14041 acggcgtaga tgtcggagat ccggaacatc cgcaagagcg tctccttgtc tccgtagccg
    14101 aacatctccg ccgccgcgtc gttgcagtcc aggagctctc cctcccgggt ggtgatgtat
    14161 accgcgtccc gggaggtcat gaagaacgag cggtagcgcg cctcgctctc cttcagggcc
    14221 tcctccatcg ccacgcgctc cgttacatcc gccacgaagt tcagggtggc atcccgtccc
    14281 tgccactgga tccggacggc gtccagttcg acccaccgcg tctcgcccgc cttccggacg
    14341 gcccggaaac ggtaccgcgg cgcgaacctc tcgccccgca ggcgtcgcag atggctctta
    14401 agcacgaatt cgcggtcttc ggggtggatg aactcggtga agggcgtgac ggccagctcc
    14461 tcggccgtgg cgcccgtcag cacaagggtc cggggattgg cgaacttcag aaccccgtcc
    14521 tggacgatga agatcgcctc gttcgcattc tccacgagac tccggtattt cgcctccgat
    14581 tcgaccagga cggcctcggt ttttttgcgc tcggtctctt cgcgggcaat caccatgatg
    14641 ccgacaggca ctccgtcctt cgataggatg gacgagcgtg catcgaaatg ccgctccgcc
    14701 cccatcgagt cccgcagggt gaacgcatag ctcaaatggt ccgtgacgcc cgcccggcgc
    14761 cggtcgatca tggtgtcgta cacggacagg gactcgggcg cgaccagctt ccggatcggc
    14821 agcccgatga ggtcccgacc gccggacatg gcctgtaccg ccggattgca gtatgtgatc
    14881 cggccctcca ggtcgatggc gacgatgcag tcatgggcgt cctctagcag ttgacgaaaa
    14941 ctcccctcgc tgtccagaag ggcgtcgtcc cgcgccccca ggtcgctgta ccgccgctgc
    15001 aggtcctgac cggacaagtc tatcttcacg cggtcccctc cccctctctc acatcccatc
    15061 gctcggccgt gtgccttgaa cgaccctgcc cgtccgtgcg ggcggccgtt ggataacgct
    15121 cggatcaaag gcccgggttc atgtataata cggatatccg tcgtcgtagc aaggaaaatc
    15181 tcattcctcg cacacccgcc aagcgaacca gggttgctgc gccttgaggg ggcaatagga
    15241 gacgccgcaa cgccggatcg gatcctcctg gggcggtttc atgcgcctcg cccccggcgc
    15301 ctgctgccgc tccacccacg cacgcgcttc gtcctccgtc gcgacgaacc ccgcgctgta
    15361 cgtggtgcgg ccgatgcaat ggagataccc gtagatcagt tcataccgct ttcccataac
    15421 gcctccccgg cggctttttt caaacctggg gaatggacaa cccatcccct cgatattgca
    15481 atccatcttt cctgaaaaaa aattcgcttg acatttctca gaatttgaat accattcaat
    15541 taacgaatgg tgcttttgtc aagaactttt ttacaggaga tgcgccatgt atgaggatct
    15601 gaaagggaaa acggccctga tcaccgggtc gggaaaaaga acgggcatgg gctacgccat
    15661 ggcggaaacc cttgcggggg cggggacgaa tgtcatcatc gccgatctgg gccggggcga
    15721 ggtgatcgac ggcgacctca agatgggggc ctgggaggag atgacccgct tggccgaaga
    15781 gctggcggaa cgcttcggcg tggcgaccct ggccgtcgcc gtggacgtca cggacaacgc
    15841 ctcgatcgcc gcgatggtgg cgaccgtccg ggagcgcttc cccagggtgg acatcctctg
    15901 caacaacgcc ggcgcctcct tcggcgtgcc gaatgcggtg ctgacctacg acgaggccgc
    15961 ctggctcaag accatcgacg tcaacctcca ctccgtcttc cggatttccc gcgccgtgat
    16021 cccgctcatg gcgggcgcgc cgggggtcat catcaacacg gcctcgcggg ccggcaaggt
    16081 cccccccatc ttcaacggcg cgtatgccgt cgccaaggcc ggcgtcatca tgctgtccaa
    16141 ggtcatggcc aaggagctgg ggggcatggg catccgggtc aacgccatct gccccgggca
    16201 gatccggacg gacctggaaa agtggcgctt cggcctggag gcgaaatttt tcgccaccac
    16261 ggtggaggag cgcgagcagg aaatgtgcaa gaccatcccg ctgggctgcc tgggcattcc
    16321 ggcggatgca ggtcatctcg tcgcctttct ggcttcggac gcctcccggt acatcaccgg
    16381 ccaggcgatc aacctggacg gcgggcaatg catggagctt taacccgaca tctcgtttca
    16441 ggaggaatgc tcatggataa aatcatcggc ggcaaactcg ttgcggacgc cctggtcgac
    16501 tggggcgtag attacatctt ttcgctgagc ggcggacaca tcactcccat ttatcaattt
    16561 ctggaaaaca cgaacattac gatcttcgac acgcgccacg agcaggcggc cgttttcatg
    16621 gccgaggcct gggcgcggat gacccgcaag cccgcggtgg cgatggtcac ggccgggccc
    16681 gggttcacca acgccctgtc gggcatcgcc agcgcccgcc tgtccaacgc cccggtcatc
    16741 ctcatctcgg ggtgcgtggg aaccgaggcg acggaaaagc tcgatcttca ggacatgagc
    16801 cagctgcccg tcattacgcc gatggtcaaa aaggccctcg tgtgccacat ccccgagcgc
    16861 atccccgaat tcatcgacat ggccttccgg acggccatcg ccgggcgtcc cgggcccgtc
    16921 tacctggagc tcccctgcaa cgtcctgaac gccaccgtga atccggccct cgtcaaaaag
    16981 atgcacacga ccctctcgtc ccagccggtc gaccgggaaa acacccggaa ggtcgtcgat
    17041 ctcctgaagg cggccaagaa ccccatcgtc atcggcggca gcggcatctg gtacgggggc
    17101 gcggaaaagg agatgatcgc gttcgtcgag aaaatcggga ttccggcctt cacggcggga
    17161 gcgggacggg gcgtcatccc cgacacgcat cccctgtgtt tcgggtcgtc gctcgccatc
    17221 cggcccgggg cggccatggc cacgctcatg accacggacc tggtcctctt cctcggcggg
    17281 cgcctcagcc tgttctacat cttcggcgaa atcttccccc cgaatgcaaa attcatccag
    17341 gtggacatcg cgcccgatga gatcgggcgc aaccggacgg tcgacctcgg catcgtcagc
    17401 gatacccggg cgtttctccg ggaactcaac ggcatgctgg atgcagaggg catcgccgaa
    17461 accctgaaga agcaatacca gccctgggtt gaaacggtcc gcaaggcgga ccaggacggc
    17521 aagaacgggg ccaaggccct gtgggaatgc gacgtccagc ccatccatcc catgcggctg
    17581 gccaaggagg tcaacgattt catgaaccgg gaggacgaca tcgtcgtcgc ggacggcggc
    17641 gatacgtcga cctggacggg catgacccga accgtcgtca agggcggcac ttacctggac
    17701 tacggccttt acggctgtct cgccgtcggc atcccctacg ccaacgccgc caagctgaaa
    17761 cacccggaca agcgggtcct gctgatcatg ggcgacggat ccgtcggctt caacttcatg
    17821 gaattccata cggcgatccg aaagaagctg cccatcgtcg tcgtcatcgg caatgaccag
    17881 tcctggggga tgatcgcgca cagccagcag ttgcgtctgg gccacaacat cccgaacggc
    17941 acggaactgg gctgggtgga ttaccacaag atggtcgagg ccctcggggg cttcggaacg
    18001 atggtggaaa aaccggagga catccgtccg gccctggagg cggcgttcgc ctccggcaaa
    18061 acggcctgca tcaacgtgaa ggtggacccg tccgtcatca gcccgggcag cgtcgccctg
    18121 gccaatctcg gcgggtacaa gtgtgaatga gcgccctgct cacgtcatcg tttcaggggg
    18181 cgccggaccg gctacccccc ttcgaaacgg gttaaggagg tttcgatgaa ccacgacgcc
    18241 gttgcccccg agggaagcaa gttgtcgaac cgaagcgctt actacatctt cacgctgctg
    18301 tttctgcttt atttcttcga ttacgtggat cgaacgatcg tgacctccgt cgcgcccttc
    18361 atccgcgaag agtgggacct cacggacatg cagtcgggcc tgctcctgtc tgtcgtctac
    18421 tggtccatcg tggcctttgt ctttcccgtc tcgatcctcg tggaccgctg gagccggaaa
    18481 aagaccgtgg gcgtcatggc ccttctctgg accttcgcga cggtcgcctg cgccttcacc
    18541 aaaagcttcc cccaactgct gctcgcccgc ggcgccatcg gagtcggcga agccggctat
    18601 gcccccgccg gtacggccat gctctccggg ctgtttccac cggaaaaacg gtcccggatg
    18661 atggggttct ggaatatcgc cattcccctc ggaacggccg tcggcatcgg gctgggcgga
    18721 ttcatcgcca cccattgggg ctggcgccat gccttcggcc tggtggccct tcccggggcg
    18781 atcatcgcca tcctgttctt cttcgtcaag gactacaaga cggtggaact ggtcaagacg
    18841 gtgggcgcgc acgacaccgg caaaaacaag atccagatga gcaagatgga catcgtccgg
    18901 gagttcatcc acaccccctc gctgatgctg accaacgtcg gcttcatcgg ctgcatcttc
    18961 gtcaacaatg ccatcatctt ctggatgccc acctattttc accggacggg cggcctctcc
    19021 atcagcgaag cgggcatgaa gacaagcctg ctcatggttc tggcgctcgt cggcctgccc
    19081 ctgggaggat ggctggccga catgtggttc aagaagcggg tgtcggcgcg gctgttgttt
    19141 cccgcgatta cgaccgcgct caacgccgtc gcgattttca tcggcatttc cctcctcacg
    19201 ggacaggcgc aatatgtgac gcttctgctc atgggcgtcc tggcctccgc cttcgccccg
    19261 gcggccatca ccgccacgca ggaggcgatc caccccgggc tgcgggcgat ctcctactcc
    19321 ctgtgcgtcg tcgttcagaa cctcttcggg gcctccatgg cgcccatcgt gatcgggaaa
    19381 ctctccgatc tctacggcat ccaaacggcg atgtccgtcc tgcccgtttt cttggtggcc
    19441 tccgccctgg tgttcctggc cggatcgttc ttctacgcgc gcgatctggc caaggtggaa
    19501 aaggtgaccc tggagtggga atgatcatgg agatcaacgg aatatgacga aacgagattg
    19561 agatgggatc gaaagagcga agagaacgag agaaggaaca gcgcaagagc cagatcctgg
    19621 acgtggcccg ggaactgctc ttggagaagg gcctgcaggc aacgtcgatc aaccagatcg
    19681 cgaagcgctc cgagctcagc gtcggcacct tctatttcta ctaccggagc aaggaagaac
    19741 tgtacgtcgc cctccagcag gaagggctcg acctcctcta cggcatgacc accgccgcca
    19801 tccggcggaa gcgaaacccc agggcgaaga tccacgccat cgcccaagcc tacctaacct
    19861 tcgcggagga gcacaggagc tatttcgaca tcatcaactt cttcctcaac tcttcctcgg
    19921 agatcttctt ctccgaacag cttaaggagc aggtcgatga gcacggcaac cggatcgtgg
    19981 acgtgctggc caatgtcatc gcggacggaa tccgttcggg tgatttccgc cccgtaccgg
    20041 cgaagcgata cgcgctgatc ctctgggcga tgatccacgg tgtcacccag ctccgcaaac
    20101 tgcgacacac catcctccgg ggggaggacc tgcacaaaat ctacacggat gccgtcgggc
    20161 ttttcatcga cgccatcggc acggcgggcg ccgggcagga ggcaccaacg ctcgacacct
    20221 gatttttgat cgactgtcgg tgggggccgc ccggcggcac accgaagaaa ggggatcgga
    20281 gatatgactg actccaaaac accgcttttg gacgagctcg aaaagggacc ctggccgagc
    20341 ttcgtcaagg aaatcaaaca ggccgcggag aagaagccgg ccgcggcgga cctgctcaac
    20401 cagctcgagc tgtcctacaa ggaccgcatc ggccactgga aacacggggg gatcgtcggc
    20461 gtcaagggct acggctccgg ggtcatcggc cgctactcgg accggccgga cctgtttccg
    20521 aacgtccagg ccttccacac gatgcgggtc aaccacccga ccgggtggtt ctacaagacg
    20581 gacgccctgc gcaagctctg cgatatctgg gacaagcacg gcagcgggct gacgaatctg
    20641 cacggctcca cgggggatgc gattctcctg ggcacgacga cggagcacct ccagccctgc
    20701 ttcgacgacc tgtcagaggc cggcttcgac ctgggcggct ccggctcggc cctgcggacg
    20761 cccagcgcct gcgtcgggcc ggcccggtgc gaatgggcct gcatcgacag cctggacatc
    20821 tgctaccagg cgacgatgac cctgcaggac cagatccacc ggcccatgtt cccctacaag
    20881 ttcaagatca aggtgtcggc ctgcgccaac gactgcgtct cggccatcgc ccgctcggac
    20941 ctggccatca tgggcacctg gaaggggcgg atccaggtcg accccgccga ggtccgggcc
    21001 tatgcggacg ccggcctgga catccggggg gaggtctgcg acctctgtcc cacgaaatgt
    21061 atggaatgga gcgaggcgga ccggaccctc gtcatccacg acggggactg caaccgctgc
    21121 atgcactgcc tgaacgtgat gccgaaggcg ctgcgtccgg ggaaggaacg gggcgcctcc
    21181 atccttgtcg gcggcaaggc cccgatcgtc cagggttcgc ttctgtcctg ggtcatcgtc
    21241 cccttcatga agctcgagcc gccctatacg gaactcttcg acctgatcgc ccgcatccag
    21301 gactggtggg acgaaaacgg ccgctcccgg gagcgcctgg gcgaactcat caaccggctc
    21361 ggcatgcggc atttcctcaa ggccgtgggc ctgcccgccg tcccccagat ggtcaaacat
    21421 ccgcggacca acccatacgt cttcttctgg cccgaagaga tcgacaagaa ggaggtgaag
    21481 tgacatggcc aagacagaca tcggtcctcc cgactacaaa cagttcctgc cgccgatcat
    21541 ccgggacaat tacggcaagt ggcggtatca tgaaatcgtc cggcccggcg tgctcaagca
    21601 cgtaggagaa aacggcgacg tcctgtacac cgttcgggtc gcctcgccgc gtctgctctc
    21661 cacggatcat atccgggaga tctgcgatct cgccgagaca tactgcgacg gcttcctgcg
    21721 gttcaccagc cgccacaaca tcgagttcct cctggcggac gagcatcgcg tcgcccccct
    21781 cattgaggaa ttgcaggcaa aaaactaccc ggtcggcggg acgggccact ccatcaccaa
    21841 catggtccac acgcagggct gggtccactg ccacacgccg gccacggacg cctccggacc
    21901 ggtgaaggcg atcatggacg agctctacga tcacttcacc cacatggacc tcccggccca
    21961 cgtccggatc tcgctcgcct gctgtctcaa catgtgcggc gcggtgcact gctccgacat
    22021 cgccatcctg ggcatccacc ggacgccccc ccggccggac aacagccgcc tgccgaacgt
    22081 ctgcgagctc ccgacgacgg tggcgtcctg ccccaccggg gccatccggg cggacatgaa
    22141 gaacaagacc gtccaggtca acgacgagaa gtgcatgtac tgcggcaact gctataccgt
    22201 ctgcccggcc atgcccatcg ccgacccgga caacgacggc atctccatct gggtgggggg
    22261 caaggtctcc aacgcgcgga gcgcccccat gttctccaag ctcgccattc ccttcctacc
    22321 gaacaacccg ccgcgctggc cggaggtcgt ggcggcggtg aagaacatcg tcgaggtcta
    22381 tgcggcgcac gcccggaagc acgagcgcct cggggagtgg atcgagcggg tgggctggga
    22441 gcggttcttc acgctcaccg gcatcgcgtt cacggacaag cacatcgacg acttcacgct
    22501 ggccacggaa accttccgga ccaccacaca gttcaaatgg gggtgatatg agcgcagaga
    22561 tcaaggaacg ggtcctcgcc tacttcaggg agaaatcgaa gggcgacaag aagatgttct
    22621 acatcaagga cgtcgtgaag ggcctgccgg acgtggaccg gcacgccgtc caagccgccg
    22681 tcaaggagct gctcgacgag gaggtcctca aatactggtc gagcggcagc acgacatacc
    22741 tgatgctggc ggaaaacttc ccgaaggatg catgacggcc cgctacggaa ccgtgcgcat
    22801 cgggtttccg cccggtagat tggcaggtaa agacatggat cgaacgcaaa ccgtttggcc
    22861 gacatccctc gtctccgccg ccgtcccccg tctcgtgatc ggggccttgc gcggaggtgc
    22921 ggggaagacg acgctcgccg tcggcatcct ggccgccctg cgccgccggg gcctcgacgt
    22981 cgcccccttc aagaaggggc cggactacat cgatgccgcg tggctctcgg ccgcggccgg
    23041 aagatcttgc cggaacctgg atacgttcct catgggcgcg gatcgggtcc ggtcgtcctt
    23101 ccggcaatat gcaccgccgt ccgggttcgc cctgatcgag ggaagccggg gcctctacga
    23161 cggactcgac gcggcaggca gccacagcac ggcggagctg gccaagctcc tgacggcccc
    23221 ggtcgtcctc gtcatggaca gcgacaaggt gacccggacc gccgcggcga tgatcctggg
    23281 ctgccagcgg ctcgatcccg cggtcgacat ccgcggcgtc atccttaacc gcaccgcccg
    23341 gagccgacac gagggaatcc tgcggcgcgc catcgaggag gcctgcggca tccccgtcct
    23401 gggggccgtc ccccggatgg ccgactaccc gttccccgaa cgccatctcg gcctcacgcc
    23461 gccgcaggag cacgaccacg taaccggggc cctgagacgc gccgcgaagg tcgcggaaga
    23521 ctgtctggac ctggaacggc tggtgtccct caccgagtcg gccccgcccc tggcggcggg
    23581 caacgctgac ctggccgacg gcgcacgccg cccggggacg gcacaccgac gacggcggcc
    23641 ggaaacccgg gacagcggac gtccggtcat cgggatcatc cgcgattcgg ccttccagtt
    23701 ctactacccg gagaacctcg aggccctcgc ggcccggggc gcgttcatcg tggagatcag
    23761 cgcctgcacc gcggccgcgc ttccggagat cgacgccctc tacatcggcg gcggctttcc
    23821 ggaaactcac gcccggatcc tggcggaaaa caaacgattc cgacaggcgt tgcgccaggg
    23881 cgtcgaagac ggtctgcccg tctacgccga atgcggcggt ctcgtgtatc tgggtgaaac
    23941 gctgacggtc ggaacggaaa cctttcccat ggcgggcgtt ttgcccgtcg ccttcggcat
    24001 ggagcgcaaa ccgcaggggc acggctacac cctgctcgag gtgacggagg tcaacccctt
    24061 ctttcccgtc gggacgacgt tgaagggtca cgaatttcat tacgcccgcg tgctggacgg
    24121 agacgcgggc ggtgcaacgc cctgctgccg ggtcattcgg ggcaggggca tcgacggccg
    24181 gggagacggc ctggtctcga acaactgcct ggcgctcttc acgcatctcc acgccgcggg
    24241 cgagccccgc tgggcggagg cgctggtgaa gcaggcggtc cgccaccggc ggacgcgatc
    24301 ccggcgggag cgggacgcga accgcgcggc acggggaccg gcggccttcc tggagggccc
    24361 ggccgggatc gggctccccc gcggaacggc gccgcaaaca caacaataaa ccatgttcta
    24421 taaggaggtt tgcacatgcc aacgattgaa ttagcgggaa agtcctacga ggtggacgag
    24481 gacgggttcc tccaggaact cgacaaatgg agtgaggaat tcgccgaggc ctacgcccac
    24541 gtggacggca tcgaagggcc gctgaccgag gaacactggc gggtgatcaa ctacctgaga
    24601 ggctattacc aggaattcgg gatcgccccg atggtgcgga aggtggtccg ggagacggga
    24661 ctcgacctga agcggatcta cgaactcttc ccctcggggc cgggcaacgg cgcctgcaag
    24721 ctcgcggggc tcgccaaacc gaccggctgc gtctaaaatc gggaccgcca ttccctgccg
    24781 gatgcggggc aagaaaagcc ccgacgggcc cgggcatttc gacgggcgca cgtcagcgga
    24841 cggcggggat ggggcaggaa taggcaggtc atgggactct acgttttaac ctatgtatgc
    24901 gcgctcgtgt tcgtcctggc gatgggcctc cggatccgga ggcagatcgc gctacccgtc
    24961 cacgtccgct gggaactcta tcccgtccgg cacgaaaccg ccgaacgggt ggcctacggc
    25021 ggctcctacc tcgaagcgtt gaactggtgg gaaaggcccg atgaacgctc cgtctggaac
    25081 gaactgaaat acatggcgcc ggagattcta ctcatgcggg gcctgtggaa ggacaaccgg
    25141 cgcctctggt gggtctcctt tccctttcac ttcggcttgt atctgatgac ggccaccttc
    25201 ggcctgctgc tgcttcacgc cctgctcgcc ctggtgggat cccaggacct cgcggagggc
    25261 catccgctcg ggcgccttct ggacgtcttg atcgtcctgg cggggtggac gggcctgatc
    25321 ctggggaaca tcggctgcct ggggcttttc catcggcggc tcgccgatcc ggaattgagg
    25381 gactacgcga gcttcgccga ttacctcaat ctcggattta tttttctgtt cttcctctgc
    25441 gccttcgcgg cgggtctggg cgatccgtcg cttgcgggcg cccgggccta tgcactcggc
    25501 ctgctgaccg cggggtccgc gccggcggca tccgttcccg gtccgtccgc ggccggaatc
    25561 gcaaccgtcg tcatggcctc actcctggcc gcctatatcc ccctgacgca catgtcccac
    25621 atgctgatga agtacttctt ctatcacaac gtcaaatggg atgacgcccc taaccgccgg
    25681 ggcggaaaga tcgaagcggc cgtccggaaa aacctcgggc tcaggccgac ctggcaggcc
    25741 tggcacgtgg gagcggacgg ccggaaatcc tggcgcgaga tcgccgcttc ggaacccgga
    25801 gagatgaaat gagaacgaga ctcgccccca cagacatctc ccggccgtcc gactgcctgt
    25861 cgcacatcga ggcggaggat cttgcgcccc ttccgccgcc ctatgaggat ttcaatacca
    25921 cggcgattcc ccccctcaag ccggagaagg tccggcacat cgagacgtcc ctggacgata
    25981 ccgtggctct cggcatcccc aagcccgcaa gtccggaaga ggaggaggcg ctggtccgcc
    26041 agttcctctc cggcctgaaa aaactcttcg agaaggaaaa caactggacg tttctgcagc
    26101 cgctgcttct gtccatggaa cactgcgcca aatgccagac ctgcgccgac gcctgccccg
    26161 tttttgaaat gagcggccgg aaggagatct accggcccat cttccgagcc gaggtcctgc
    26221 gccggattta caaaaagtat ctgaggccgg gcgggaagat cacggccaag ctgaccggcg
    26281 acatcgattt gaactggacg gcgatcgcgc gcctggccga gttgtcctat cgctgcaccc
    26341 tctgccgccg ctgcgccgcg gcctgcccga tcggcgtgga caacggcctg atcgcgcacg
    26401 agatccgcaa gctcttctcg caggaaatgg gcatcgcccc caaggaactc cacgaaaaag
    26461 gaaccgtcca gcatcttcag gtcggctcct ccaccggcat gaaacccccg ggcgcgaagg
    26521 acagcctcga gttcatccag gaagagatgg aagagatcac gggctacaag gtggacatcc
    26581 cctgggatgt ggaaggcgcg gacatcctgc tcatccacaa tgccggcgag atcctggcct
    26641 ggccggacaa tgtcggggcg ttcgccgtca tatttcaggc ggccggcgtt tcgtggacac
    26701 tgtcttcgga ggcggccggt tacgacgtgg tcaactacgg gctgtggtac gacgatgtcc
    26761 agttttcccg tgttgccgtc cggcatgccg atatcgcccg ccgcctcaag gtcaaaaaga
    26821 tcgtcatcgg cgaatgcggc catgcgcaca aggcgctcac ggtcgtcgcc gaccgcctgc
    26881 tgacgggcga tctcaacatc ccgcgcgaat ccgccttggt cacgctcgcg gacatcgtcc
    26941 tcagcggtaa attgcggctc gatccccgca gaaacgattt ccccgtgacg ctgcacgatc
    27001 cctgcaacat cgtgcgttcc atggggatcg tggcgcccca gcggcgcatc ctgaaaaaga
    27061 tcgcgccgcg cttccgggaa atgacgccgc acggggtgga gaattactgc tgcggcggcg
    27121 gttccgggtt cgccatcatg tcgggacaca acttcgtcga ctggcggaac cgggtcagtt
    27181 cccgcaagaa attcctgcag atcttggccg ccttcgccaa cgagccggcg gaggtcccga
    27241 agtacgtctg cgccccctgc tcgaactgca agggcgccat ccgggatctg atccgctatt
    27301 accacgcgga ggaacgcagc cgcctgtatt acggcgggct ggtcgacctg atcgtcaatg
    27361 ccatggcgga catgaaaaag ccgatcatcc gcttcggcga cgaaccgtcg tggaaagcct
    27421 gatcaaacga acgaggcaga aggaatcgcc ctcatgaccc ttttcgcacc gctcatcgcc
    27481 atcgccctcc tgatcgccct ccccctcgcg ggactggcgg cgagcgggga aggtttgctg
    27541 gccgtcggcg tcccctacgc ggccctggcg gtgctcgtcg tcggattcgt ccggcgcatc
    27601 gcccgctggg cgaacgcgcc cgtccccttc cgcatcccca cgacggcggg gcaggggcgc
    27661 tcgctcgacg ggatcaaacc ggaccgcctc gacaaccccg cgacgacctg gggcgtcctc
    27721 ggccggatgg cgcttgaaat cctctgtttc cggtcgctct tccggaacac gaaacaacgc
    27781 ctggcgcctc cggcgcgggc ggaaagcgcc ggggacaaag cggcggccac ggcccgaccc
    27841 gtttatgtca gcgccaaggg cctgtggttc ttcgcgctgc tctttcacgg gtcgcttctg
    27901 gtgatcgtgc tccggcacct gcgcttcttt ctcacgccgg tcccgacctg ggtcgacggc
    27961 ctgatcgcgc tcgacggcct cttcgccgtc ggcctcccga ccgcctacct gtccgacgcg
    28021 gcgcttctcg cggccctctt gttcctgctt ctccggcggg tcctgatcca gcaggttcgc
    28081 tacatcacgc tggcggcgga ttattttccg ctgttgttga tcggcgcaat cgccgtttcc
    28141 ggcctcctgc tgcggcatgc cttcaaggtc gatctcaacc aggtgaaggc gctcgcggtg
    28201 ggctgggcca ccttttcccc ggcggtcccg gcggaaacag aggcgttctt cttcgtccat
    28261 ctcttcctgg tgtgcgccct ggccgcctat ctcccgttcg gaaaactcat gcatggggcg
    28321 ggcgttttcc tgagccccac ccggaacctc gccaacgaca accggcggcg gcgtcacgtg
    28381 aacccctgga acgcgccggt gaaggtgcat ccctacgaag aatacgagga cgaattccgg
    28441 ggcaagatga aggcggcggg catccccgtc gagcgggagg aaggatcatg agacggttag
    28501 actcccgttt cgcgaagggc gaaacggaga cttcccgcag aaatatcgac aggacgtgca
    28561 accatggcaa tcgataaact tccgacgccc gaagaactga gccatatcga ctaccggcct
    28621 cccggaaccg gatggcggaa caccccggtg gatttccgtc cggggacctg gtgctacagc
    28681 gccaccccga aaagcctgac caccctggat ctccccaatc cccggcagtg gcagccgtcg
    28741 gacgcggact ggaagctccc tgagaactgg cgaacaatca tcctcgatgg catgcgggag
    28801 cgcctggaga agtaccggtc gttccggctc ttcatggaca tctgcgtccg ctgtggggcc
    28861 tgtgcggaca agtgccactt ctacatcggg acgggagatc cgaagaacat gccgatcctg
    28921 agggcggaac tgatccggag cgtctaccgc cgctacttca cgacggccgg gcgcatcctc
    28981 ggccctctcg ccggggcgcg ggacctgacc gaggacgtcc tgaaggagtg gttctactac
    29041 ttctaccagt gcacggagtg ccgccgctgc tccgtcttct gcccctacgg catcgaccag
    29101 gcggagatca cgatgatcgg ccgggagctt ctgaccctcg tcggctgcaa catcaactgg
    29161 gtcctcgaac cggcggcgaa ctgctttcgg acggggaacc acctgggcat cccgccccac
    29221 gggatcaggg atatgctcga cttcctcgtc gacgatatca aggaccgcac gggcgtccgc
    29281 gtggaggtcc ccctgaaccg gaagggcgcc gagatcctct tcgtcacgcc gtcgggggac
    29341 tactttgccg acccgggcac ctacacctgc atgggctacc tgatgctctt ccacgagctt
    29401 ggtctcgact acacgatgag cacctacgcc tccgaagggg gaaatttcgg cctgttcacc
    29461 tcccacgaga tgatcaagcg cttaaacgcc aagatctacg ccgaggcgaa gcgcctgggc
    29521 gtgaagtgga tcctgggcgg cgagtgcggc cacatgtggc gggtcgtcca ccagtacatg
    29581 gacacgatga acggcccggc cgatttcctc gaggtccccg tctcgcccat cacgggaacg
    29641 cggttcgaaa acgccgccgg gacgaagatg gtccacatcg ccgagttcac ggccgacctg
    29701 atccaaaacg gaaaactcag gctcgacccc gcccggaacg accacctgag ggtcaccttc
    29761 cacgactcgt gcaaccccgc gcgggcaatg ggcctcctgg aggagccgcg cacgattatc
    29821 cggcatgcct gcaaccattt cttcgagatg ccggaagata cgatccgtga aaagaccctc
    29881 tgctgcggtg gcggggcggg actgggaacg gacgagaaca tggagatgcg gatgcgcggg
    29941 ggctaccccc gggcgatggc ggtgaaacat gtccgggaac ggcacggcgt gaaccgcttg
    30001 gcctgcatgt gcgccatcga ccgggtaacg ctaccgcccc tgatggatta ctgggtcccc
    30061 ggggtcgggg tcacgggcgt gcacgagctc ttgggcaatg ccctgatcct gacgggagaa
    30121 aagcaacgga cgacggacct gcggggagag cccctcctcc gggcgtcgca tgcggacggg
    30181 gtacacgaag cgaagggatg aagcggcggg gcgaggcgga cccgttcggg ggcgcctgcc
    30241 gccgggagag gatcatcgga ccatgaaggg aacggatcgc tggaaaatct ttgcggggtt
    30301 gttcgtcttc ctcgcgctcg ccgccctgcc cttctggtac ggcgggtggg acaggccggc
    30361 gccggtcctc gatctcgaca ccccggccat ccggcaactg ccggaaaggc gatgcgtcga
    30421 ggccaccgcg ttcatgcggg cgaaccacat gacgctgctg gccgactggc gggacgcctc
    30481 gacccgggac gggcggcgga tctacgtcgc gcgggacggg cgggaaatcg aggcgagcct
    30541 gtctcagacg tgcctgcgct gccacaccga caagacccgc ttctgcgacc gctgtcacga
    30601 cgacgcgggc gtgagtccca cctgctggga ctgccacgtc gtcccggagg accggcgtga
    30661 atcggcgacg gtcacgcaca ggcagagggg ggaagggggg gacgtgtccc cgttccccgt
    30721 tgacgttgcc gcacgggaaa aagaaagccg gggacctggt ccccgatgaa acttgcatgc
    30781 agggacagag gttatgcaca gtagccgcag agactttttg aaactgacgg gcattgccct
    30841 gatcggcgcc gccgtccccg acgcgctgct tccggccggg cttgcgccgg catgggcggg
    30901 agcacccggc ttccgcccgg atgtccgcgc gctcaaggcc gcgcgctggg ggatggtcat
    30961 cgacctcgcg aaatgccgcc cggactgcac ggactgcatc gccgcctgcc acgcggccca
    31021 caacgtcccc gctttcggca atccgaagga cgagatcgcc tggatccgcc gggagacctt
    31081 cggtcggatc ttccccgcgc aggtccacag gcacctcgaa ccccggcgtc aggcggggcc
    31141 ggtgatcgcc ctgtgcaacc actgcgcgaa tccgccctgc gtccgcgtct gccccacggg
    31201 ggcgaccttt cggcggcccg acggaatcgt catgatggac taccaccggt gcatcggctg
    31261 ccgtttctgc atcgcggcct gcccctacgg cgcccgcggc atgaactatc gcgatccccg
    31321 gccgttcatc cgccgcatcg atcccgggtt cccgacgcga acgaggggcg tcgtggagaa
    31381 gtgcaatttc tgtgtggagc ggctggccgt gggaaagccg cccgcgtgcg tcgcggcctg
    31441 tccctacggc gccctgactt tcggcgacct ggaggcgccc gactccgcgg tgcggcgcgc
    31501 aatcgccgga agaacggtcc tccggcgtaa accggaactg ggcacggagc cccaagttta
    31561 ttatacgtta taagatgagg aaccggggga tgacatgcta gagaaggccc tgcacggaaa
    31621 tcaccgctac tggggatggc tcgcgttcct cgccgtcgtc gtcggcgcgg gcgcgttttt
    31681 ctacctccgg cagctcgact acggcctggg catcacgggg atgagccggg acgtcacctg
    31741 ggggctctac atcgcccagt tcacgtttct ggtcgggatc gccgcctcgg ccgtcatggt
    31801 cgtcctgccc tattacctcc acgactacaa ggcgttcggc cggatcacga tcctgggcga
    31861 attcctcgcg gtcgccgccg tcgtcatgtg cgtgaccttc atcgtcatcg acctcggccg
    31921 gcctgaacgc gccttcaacg tcctgctgca ccctgccccc cgctccatcc tcttctggga
    31981 catggtcgtc ctgacgggct acctcgtgct caacctcgtt atcggctgga cggtcctgga
    32041 gaacgagcgc aacggcaagg cgccgcccgc ctgggtcaag cccctgatcc tgctttcgat
    32101 cccctgggcg atcagtattc atacggtcac ggccttcatc tacgccggcc tgccgggacg
    32161 ggacttctgg ctgacggcca tcatggcccc ccgctttctc gcctccgcct tcgcctcggg
    32221 gcctgcgctg ctcgtgctcc tctgcctcgt tctgcggcgc tggggcggct tcgacgcggg
    32281 ccgcgagccg ctccaggcgc tggcccggat catcgcctac gccttggcgg cgagcctctt
    32341 cttcgtcctc atggagctct tcacggcctt ctacagcggc atccccggcc acacggaacc
    32401 gttccgctac ctctacgccg gtctggacgg gcacacgcgg cttgtcccgt ggatgtggac
    32461 ctcggccgtc ctggcggtcc tggccctcgt gctcctgatc gttccggcgc tgcggcgcaa
    32521 cgaagcctgg ctcgccctgg cctgcctggc cgtcttcgcc tcgctgtgga tcgaaaaagg
    32581 gctggggctt gtcgtcacgg ggttcatccc ctcccccctg ggccagatca ccgaatacac
    32641 gcccaccggc cccgaggtcg cgatcacgct gggcgtctgg gcgctgggat tcgtcatcct
    32701 gacggccctc tacaggatcg ccctgggcgt caggaaggaa acggaggcat agacgatcat
    32761 gaagaccctc tacgtcgaga ccacgcccgc ccgcttcctc gccgtcaaga ccctccgcgg
    32821 gctctggccg ggcgtgctgt ggagccgcct gtccacgctc cagtatcggg agatgacgcc
    32881 gaccgccccg ccggccggct gggtccacgt gaaaaatcac caaggcggaa tctgcggaag
    32941 cgatctgcac ctgatcttcc tcgacgccga tctgagcgtc cacccggccc tcctgccggg
    33001 cagcacacgc aagtacctgg ggcacgaact cgtggggcac gtcctggaag cacccccgga
    33061 cagcggcttc aagcccggcg accgggtcat ccggcagatg cgcaccggcg gcggctcctg
    33121 cctggcgcat ggcctcgccc cctgcggttt ctgccgggcg ggtcagtatc accaatgcga
    33181 gcgaacggga ctcccggatt ccatcggcgg gggactgagc gacgagcttt acctccccgc
    33241 cgggggcctg atggcggtcc ccgacgatct cgcggacgcg caggcggttc tggtcgagcc
    33301 ggcggcctgc gccatccacg gggttctgcg cgcaccgccc aatcccgggg aggcggtcct
    33361 cgtctacggg gcgggcacga tcgcctattt cgtcctgcag gtcctccgaa tcgtccttcc
    33421 ggactgccgg atcacggcga tcgccgaatt cccctttcag gaagacatgg cccggcggtg
    33481 cggggcggac gaggtctggc tggcgcacga cgatctcctc gccaggaccg cggcgcggac
    33541 gggggggcgg gtcgtcaccg gccgggcaaa ccgccggacc ctcctgggcg gcttcgaccg
    33601 gatctacgac tgcgtgggtg tcgccgcgac cctcgggctc tccctgcgcc tcgcgcgggc
    33661 gcgcggcacc gtcatcctga tcggcgttac cctcaaaccc ctgacgctgg acatgacgcc
    33721 ggtgtggtac aatgaggtga acctggtggg cagcgtctcc ttcgccgccg aagcgtggcg
    33781 aggggagcgg atccgggact tcgatctcgc catccgctgg atgcgggagg ggcggctgaa
    33841 gacggagggg ttcgccacgc accgcttccc gctggaacgg taccgggagg cgatcctggc
    33901 cgccgtgcgg aaggcgtcca gccgctccgt gaaagtggtg ctggagatgg agaagagctg
    33961 agggcgaacg gtcaacggcg taaagcgaag ggaacggacc aaggcgcagg tcaagcgacc
    34021 ccggggaaaa gatttgaata caaaaatggc catgaagatt ctggttctca actgcggaag
    34081 ctcgtcggtg aagtacaagc tctacgacat ggaaagcggc cgggtggtgg cgctcggctt
    34141 tgcggaacgg atcggcctgg cgggctcagg aatccgtcat caggcggagg gcgcggcggc
    34201 cgtttccttc gaacacaccc tgcgggatca ccgggaagcg ctggaaatcc tactcgggat
    34261 gcggcccggc ggtccgcgtg cgggcagcat cgaccccgcg gcgatcacgg ccatcggtca
    34321 ccgggtggtc cacggcggcg aatatttccg gacccccgtg atcatcgacg acgcggtcct
    34381 cgtcaacctg aaggcgtgca gcatgctggc ccctctccac aacccccaca acctggcggg
    34441 aatcgaggtc tgccggaaac tgctgacgga ggtgccgcag gtggccgtct tcgacacggc
    34501 cttccaccag accatgcccc ccctggccta cacatacgcg atcccgcatc gttactacga
    34561 cacctaccgg atccgcaaat acggcttcca cggaacctcc caccgctacg tggccgagcg
    34621 cgccgccgaa ctcgcgggcc gtcccctggc ggagctgaag atcgtaacct gccacctcgg
    34681 ggcggggtgc agcctctgcg ccgtccgcga cgggcactcc atcgatacga cgatgggctt
    34741 cacgccgctc gcgggtgtcg tcatgggaac ccgctcgggg gacatcgatc ccgccctcgt
    34801 cccgttcatc gccgacaagg aaggcctgcc gcccgccgcc gttatggaca tcctgaactc
    34861 cgaaagcggg gttttcggga tctccgggat ctccggcgat ttccgggatc tcctgaaggc
    34921 cgcgcgggag gggaacgaac gggcgacact cgccctggac gtcttcacgt acagcatcgc
    34981 caaggggatc ggttcgctcc tgccggccct ggagggactc gacgccctcg ttttcacggc
    35041 cggcatcggc gagaactcgc ccgacatccg caggctgatc tgccggcgtc tggcatattt
    35101 cggcatcacg ctggacgatg ggaagaatgc ccttgcccga acggaaacgg acgtcgcgac
    35161 gacggagagc cgggtcagga tctacgtcat cccgacggac gaggagcgca tgatcgcccg
    35221 ggaaacgcgg aagctcatcg atcctgggcg gggacccggc tgaagggcgg cagcggcagc
    35281 atcccccgcc ggggaatcaa aggggtcaac cggacaacga acgaaattca acgcaatgga
    35341 ggtatcaaca tggccaagat caaaatgacg cccagtgagg cgattgtgga aaccctgctc
    35401 gccgaaggcg tggatcacgt caccggcatc gtcgggagcg cctttatgga catgctcgat
    35461 ctcttcccca cggcggggat ccgtttcatc cccgttcggc acgagcagag cgcggggcac
    35521 atggaggacg cctacgcccg gatcaccggc cgggccgggg ttctcacggg tcagaacgga
    35581 ccggggatca cgaacatggt cacctcggtc gcggcggcca acatgggcca tacgccgctc
    35641 gtcatcatct ccccgtcggc cgggacgccg accatcggct gggacggttt ccaggagtgc
    35701 gaccaggtct ccgtcttcaa ggccatcacg aaggccacgg tccgcgtgcc ccatcccaag
    35761 cgggcggccg actgcctgcg gaccgccttc cggatcgcgt acgccgaccg tggggcggtc
    35821 ctgtacgaca tcccccggga ctacttctac ggcgagttgg aagaggagat ccttccgccc
    35881 caccgctacc gcgtggacgc ccggggctgc ggatcgctcg ccgacatcga gcgggcggcg
    35941 aagctcctca tgaacgcgaa gaaccccgtc atcgtctccg gacggggggt cgtggacgcg
    36001 gacgccctgg acgtgatcaa ggccattgcc gagcacctga ccgcgccggt cgccgtgacc
    36061 tatctgcaca acgacgcctt ctacggggac caccccctgt tcgtcggccc catcgggtac
    36121 atgggctcca aggcggcaat gcggaccctg gccaaggcgg acgtgatgct cgccgtggga
    36181 acgcgcctgt cggtcttcgg caccctgccc cagtacgaca tcaactactt cccgaagaag
    36241 gcgaagatcg tccaggtgga catcaacccg aaacacatcg cccggacgca ccccgtcgag
    36301 atcggcatca tcggggacgc gaaggaggcc gcggtggaga tcctgaagcg cctccggacc
    36361 gcggacaagc ggcggaagcc ggatacggcg cgcctgggga aaatcgccgc ggagaggaaa
    36421 aaatgggagc aggagatcgt ccggttggcg agggtcaagg gaacgccgat caacccccgc
    36481 cgcgtccttt tggagatgtc gcggatgatg ccggacaacg ccatcctgac gacggacatc
    36541 ggcaacgtgt cctcgaccgc caacagctac ttcaccttca agggcggccg gcgccacatc
    36601 gcggcactca ccttcggcaa cacgggcttc gcctacccgg cggccctggg cgcccagctc
    36661 gcccgcccca aggcgccggt cgtcgccatc atcggcgacg gggcctgggg gatgagcctc
    36721 cacgagatca gcaccgccgt ggagcacaac ctgccggtca tcgcctgcgt cttcaggaac
    36781 atgtggtggg gcgccgaggc gaagaaccag gtggacttct acaacaaccg cttcatcggg
    36841 gtcgacatcc cgaaccccgc gagcttcatc ccggcggcgg aggccctggg cgcccgcggc
    36901 atccgcgtgg agcatccgga ccagatcggg gaggccttca aatcggccct caagatccgc
    36961 cggcccaccg tcctggagtt tgtcgtggac ggccggcagc tcgccccgcc cttccggaag
    37021 gacgccctgg cgctgcccac gcggctcctg ccgaagtacg cccacctgga ccaccacaac
    37081 tggcggacgg actacgacat gtagccccct cccgaaacaa ggccgcggac ccggcggggg
    37141 ttgttccccc gatcgggtcc gcggatgctt cccgccccag cggctgcggc acaggcaccg
    37201 gctccacgct tcgggtcgat agggtcacgt tcacttgcct gcgggaagaa gcgccgctcg
    37261 aagcccccgt gacggtatga tccctgcagc aaatgaccca acgaaaagat tgaattgaca
    37321 tccggggcgg gcttccctat agtgccgccg tccacagccg agacgtcgcc cttccgggga
    37381 gggtcccgtt tttcagtgca acgtcgccat cactgcagat gcggcctttc gatcaacccg
    37441 agaccctccg ccccatggaa aggaatgcgc atgttgtatg aattcgacgg cagaacccca
    37501 caggtcgccg acggcgccta tgtcagcgac accgctacgg tgatcggcga cgtcgtgatc
    37561 ggcccccatt gttacatcgg tcacggcgcc atcctgcggg gggactacgg ccggattgag
    37621 atcggtccgg gaacggcagt ggaggagggc gtcatcatcc atgccccgcc ggaacgggtc
    37681 tgccggatcg gtgaaaaggt caccctggga cacggggcgg tcattcacgg ccagaggctc
    37741 ggagattgct ccgtcgtcgg catgggtgcg gttttgagca tcctctccga gatcggcgaa
    37801 tggaccatcg tggcggaggg ggccgttgtc aagatcggcc aggcgatccc ggatgggatg
    37861 gtcgccgtgg ggaacccggc ggccgtcgtt cgcgagatcg ccgaccggga ccgggagatg
    37921 tggagctggg gcaagcagat ctacatcgat cttgcggaga aatacctgcg ggtcggcctt
    37981 catcccgttg ctccggacgg gcggaacgga taacgaggcc cgcccctctc gggatgacga
    38041 tgaagcgaac gcgacttcgt ttcggcagaa gacttggaga cgaccgtttc agcccctcgg
    38101 gagcccggcg agccgttcct ttaactcatt cgcaatatcg ctcatttctg cgcggccaaa
    38161 tagattattc atctctcccg gatcgctttt cagatcaaaa agccaaggac gtctgccgcg
    38221 ttcaaactca atgtatttga aattttcggt acgaatgccc cggtatgatg gcgtgttttc
    38281 cgggtaatag cgccagaatt caagaaggca ggcctctctg gttttcccgc gggggttccc
    38341 gagaacaggg gcaaggctga taccgtccat atctccggga acctcaaggc ccgccaggtc
    38401 aagcatcgtc ggggcaacgt caatattcaa gatcatctga tgtctccttc ccccggggtc
    38461 atgaatgatg acgggtgccc tcacgataaa aggaaggcgg attgaatcct catagggttc
    38521 gcgaattccg tggcaatgat gggttcccca ctgcatgcca ttgtcaccca tgtataccac
    38581 gatggttttc tcgctcaggt tgtgccggtc cagataatcg agaattcgga cgatatcccg
    38641 atccatggcg gtaagggttt cgcagtagcg tcgatactga tcctcatagg agcccatcat
    38701 gatgccctgg aagacattcc ccctcgtttt cccaaaccat atcgggtcga cgttcggcgg
    38761 aagcacccgg ctcacatccg cgtcatgata cattccagca atgtccgcgg gcgcctgaaa
    38821 gggcggatga ccgggccggt gcgcgagata gacgcagaag ggtctgccgt catttcgcga
    38881 tctctgctgg tcaataaact cgatcgcgta atccgtcact tcctcggtga tatagggctt
    38941 gcgggaaggc acttcgcggc cattgacaat catcgggcag ttgaagtagg caccctgccc
    39001 ctcgcgatag gtgtaggcca caaaaagatc aagaaagggg agatggggca agccgccggg
    39061 catgtgccac ttcccgatga atgccgtggc gtatccctca cgactcagat attccgggaa
    39121 ggtcgtgaac ctgcctgtcc aagcggtatg attgttcctg acgccatgcc ggtgggcata
    39181 ggcccccgtc agaatgctgg cgcgggaggg gctgcacaga gcggtggtat tgaaggcctg
    39241 ctcgaaaaga accccttccc gggccagcct gtccattccc ggggtttgaa tgaagggatg
    39301 atgcgcacag cccattgttt ttgcgttatg attatcgccg aggataaaca ggatattggg
    39361 cctgttacca ccccgcctgc cgcgagatcg cacaaggggg atgcccgcct tcgctttttc
    39421 acccaagggt cgttccgtgg caccgagcct atctcccagc aggccgtatg ccgcatatcc
    39481 cgcaaagccg gccccgagga ctcttcctcc ggccttcaaa agatcccgtc tggttaaaac
    39541 agggcgttta ttttctctgt tcatcatcgt cccccgccga gcggcttgcg ggttgctcca
    39601 cgttgttcct acaagccgct tccaaaaaag aaccggtatc cttcaagaaa gatcacatag
    39661 atattttcgc ccctgcccca ggcgtacatc agggcaaaga ccgtgaggct cccggcaatg
    39721 atcagcgcgg gaactctcat ggagtatgcc ttttcattcc tttggatgcg gatctttctt
    39781 acaatgagcg ccgcgacacc taactcggca cacatcagga caaaaagcca gatgctcaga
    39841 gggctgagat accagtaaag gcattcaagg acggggtggg attccgtcag gatgagcact
    39901 tcctttgtaa tccacgcaaa aggtgtgaca aatgaagcca ggacactctc cgccaaagag
    39961 gccccccgaa gctgggaaac cgtatagaca tacaaagccc cgaaggaaat cgagacaaaa
    40021 tagaaaaagc ctgaaagagt cgcaacaacg agggtgaatg ttgtccggtc gggaaacctc
    40081 aggaaaaagt aaactgcgaa cgaaagcagg gccgcaacaa aaacgggcaa gaccggcatc
    40141 cctacgcgca aaataaatga tttccgtccc atcgcctcac catcccaatc ggaaactctc
    40201 ttcacattga tttgcggcgt ccatataagc ttatcggcac gagatgaagt cacgtcgttt
    40261 atatttccgg gacttccggc ggaagaaagc gggtggagga agtcccgcgg tcatacacaa
    40321 acggacacgg ttgataccga gacggcggaa ggaatgatgt taagtttacc atggacaggc
    40381 gaagaaggcc agcgcgattg ccgaggcgga taaaatcttc ttgacacaca caagagaccc
    40441 gaatacactc cactctacac gagtgaaatt cttatccatc cttggctttc caccgcatga
    40501 tgaatatgtg ttcattttat gaatcgggat tcattgaatg agggaatggg gatgatacag
    40561 atcgcacttc ccaacaaggg cgcttcgcgc gatggggtga tgagggagct caaggccctg
    40621 cattccgcgg acaacgattg gcggacgggg cggatgttca gcctcatcta caacgccggg
    40681 gccgatgtgg aatcggtcgc ccgggaggcc tacgccgagt acatggtgga gaacgcgctg
    40741 agccccttct ccttcccaag cctcctcaag atggagacgg agttgctctc catgatcgcc
    40801 acgctctttc acggggagac ggccgtgggc agcatgacct cgggcggatc ggagagcatc
    40861 ctgatggcgg tgaagaccgc gcgggaatgg gcgcgcgcga agcggccggg gatctcctcc
    40921 cccgaactgg tcatgccggt caccgcccac ccggcgttca acaaggcggc ccacttcctg
    40981 gggatcaggg cggtggcggt ccgcgtcgat ggcggattcc gcgcgagcgt cccggacatg
    41041 atcggcgcgg tcacggagaa caccatcatg atggtgggga gcgccttcac ctatccccac
    41101 ggcatgatcg atcccatcgg cgagctttcc ggcttcgcgc ttgaaaacaa catctggttc
    41161 cacaccgacg cctgcctggg agggttcatg attcccttcc tcgaaatgga gggatacccg
    41221 gtgcgcccct tcgacttcag gcttcccggc gtcaagtcga tctcggccga catccacaag
    41281 tacggataca ccccgaaggg ggcgtcgacg gtcacctatc gcgacatgga tctcagggag
    41341 ttccaattct tcgcgtacgc ggactggccc ggcggtgtgt acgggacggc ctgcgtctcc
    41401 ggcgctcgcc ccgggggggc catctcctcc gcatgggccg tgatgcgcta cctcggccaa
    41461 gaggggttcc gcgggcttgc gtcgaaggcc atgagggccg ccaagcgtct cagggcgggt
    41521 atcgaggcga ttcccggcct ccatgttctc gggaaccccg acgggaccgt cttcgctttc
    41581 gggagcgacg ggttgaacgt ctacgacctg cgcgacgccc tcaagcggcg gaactggcac
    41641 gtggagagcc agcacctccc gccctgcctg caccttaccg tctgccccgt ccacgaggag
    41701 gtcgtggacg cgttcctcga ggatctcgcg gaggcctgcg gcgaggcggc ttccgtgagc
    41761 gagaacgaca ggaccgaaga cgcggtgctc tacggggtga tggggaccat ccccgacagg
    41821 agtcaggccc tggatctggc ggtaaaatac ctcaacgacc tgtacaggat caagccataa
    41881 cgcacgaaat gctcctgatg atcgggttcc tggtgatcgc ccacgcggtg gagtccgtcc
    41941 tcggcttcgg cgccacggtc atcgccttcg gcctgggggc gcatttcttc cctgccgacc
    42001 ggctggtggt gatcctggtg ctcattgcgc ttgttcagag cgcctacctc atggcacgat
    42061 ggttccgcga catcgagtgg ggaatcgtct tccggtccat cgctcccgcc gcgctcatgg
    42121 gactggcggc tggcatcctg atccgcagca tggtcaacga agggatgctg aagttgatcc
    42181 tgggggggtt catcgtcacc ctctcgctta tcgagctgcg cttcctcttt ctgaaaaagg
    42241 acgctccccc ccgcgatcct tcggcggtca cgggatccct cctggtcttc ggcggggggg
    42301 tgttccacgg ccttctcgcg gtcggcgggc cgctgatcgt gtactacgcg ggaaggaagc
    42361 tcgcctccca ggagtcggtg cgcggaacac ttgcattcat ctggatattc ctcaacacga
    42421 tcatgctcgc gggattcatc gcggacggga gcattaccgc ggacgttctc tctaccgcgg
    42481 gtcttctgct tccgggggtg ctcgcgggca tcctcatcgg caacaggctg aaaatcagcg
    42541 acaggctctt caagacggca acgtacctgc tgcttctcgt ggtcggcgca acgctggtcc
    42601 tgccatgacc cgcggcggcg ctccgaagac cttcaaccag gaggcgccat gagcttgatt
    42661 cttctgagtc tcaccttcct tctgtttccc gccgtggtga tctatgcgtg cggccggttc
    42721 cccgcgatcg acaagatcgg agcgggcatc atctgctacg gtgcgggcat cgccctgagc
    42781 ctcgccggac tcgttcctgc gggcgcaagg ccggtccagg aggcgttcat gaacgtgtcg
    42841 gtgccgctct cgataccgct gatgctcttc tccgtagacc tcaaacgctg gtcccgcctc
    42901 gccggaacga cggcgacttc cttcgcgtgc atgatcctcg ccgtcctctg cgccacggcg
    42961 gcgggttttc tcattttcga ggaccgtctg cccgaggtgt ggatgatagc gggaatgtcc
    43021 atcggcgtgt acacgggcgg gacgcccaac ctcaacgcga tcgggctcgc gctgaaggcc
    43081 ccggagcaca tcatcgtcct cgcgaacacc gccgacatgt tggtatgcat cccctggttc
    43141 atcttcatcc ttaccttcgc ccagcggatc ctcaatctct tccttccccc tttcgagaag
    43201 catctcgctt ccccggccgc ggacggtcgc agcgcaccgg ataccgattg ggagacgaaa
    43261 gacatcaatg actaccgcaa catcttctcc cggccggtcc tactcccgct gacggggggt
    43321 ctctgcctcg cgctcaccat cttcgcggca ggcgtaggcc tgtattcaat cgccccgcgt
    43381 gagtacaaca tggcggtcct gatgctggcc atcactaccc ttgggcttgc ggcctcgttc
    43441 atccccgcgg tccggaacat cgacaagacg ttccagctgg gccagtatat catcctggtg
    43501 ttctgcctcg tggtgggcag catggcggac atccggcagc tcgtgatcgc cgccccgcat
    43561 atcatcctgt acatggccct ggtcgtgtac ggctcatttg ccctgcatct cttcatgagc
    43621 cggattttca aggtcgacac cgacacgacg atcatcacgg ccaccgcggc catcttcagc
    43681 ccgcccttcg tacccgtggt cgcctcggcc ctcaggaaca gggaggtcgt catatcgggc
    43741 cttaccgcgg gcatcatggg atacgtcatc ggcaactatc tcggcatcac catcgcctac
    43801 cttctgaggg cctggggagg ataaccgcgg acgggaggcc tcgaccgcgc cccttgtctt
    43861 tccgccatga gacaaaacgt tcccgggaca aaaaagggca ggacctggat gatcctgccc
    43921 ccgggaacag cccgccaaaa agacggcgtc cggttcagta cttcccaccg atataatcgc
    43981 tcaggtgatc gatcagcgca tccttctggg cgatctgggc tttgacggca tcgccgatcg
    44041 agatgaggcc gagaaatttg ggcccgtcga aaacgggcac atgacggata tgtttcccgg
    44101 tcatcagcac catgcaatcc tccacccgcg tatcgggctt taccgtgtac attttctcaa
    44161 acggcgtcat cacctctttg acccgggtat cgagggaatt cttgcccttg atgacgattt
    44221 ttctcgcgta atcccgttcc gtgaagatac cgacaacctt ctccttcttg tccataacca
    44281 ccagcgcccc gatttccttc tccgccatga gcttcagggc gtcgagcacc gaagcctccg
    44341 gaagaaccgt ccacaattgc ttcgatttgc ccttcaataa atcgctcgct ctctgcattg
    44401 ttccatccct ccccttgaat tcagaatgtt atccgaaaag ccggttctta ctttaaaaga
    44461 aaacggggaa tttggcaagc ctttgtgtca ttcggcttgc ccgttgggaa gcgcccaagg
    44521 aacgatgagc gttcttttcc cccgccaaac cgccatccgc gccgtttcat tttgaggttg
    44581 ccgcagcaga cgatttatgt catgaattgc gttatctcag gaaaggagga tgagcgtcat
    44641 gcgagcgaaa acgtggtccc cttacgttgc cggcgccttg gccggtctgc tgctggtcct
    44701 gtccgtcttc ctcacgggga agtacttcgg ggcctcgacg accttcgtcc gcgccgcggg
    44761 gtttgtcgag cagtccgtcg cgccggaaaa agtggccggc atggagtatt tcctcaagga
    44821 gaaggcaaag ctcgactggc agggaatgtt tgtcctggcg gttctcttcg gttctctcgc
    44881 ggcggcctcg ctgtccggtg accggcgggc ggtcccggta cccgcgatgt gggaggcgcg
    44941 cttcggggct tcccgcgtcc gccgctggac gctggcgttc ctcggcggcg tgatcgtgat
    45001 gttcggcgcg cgtctcgcgg acgggtgacc gagcggtcac ggtctgagcg gtctggctca
    45061 gatagccatc agcgggtaca tctctctcgt ctgtttcttc ctggccggca ttgtcgtggc
    45121 cagaatcctg tatggaggag gtcaacggcc atgaacgaac ttctgaccgg cgccgtcacg
    45181 ggccttctct tcggattcat catgcagaag gcccaggtca tccgttacga tcgtcagttg
    45241 ggcgccttgc gcctgcagga catgaccatc gtcaaattca tgctgagcac catcctggtc
    45301 gccatggtgg ggatctacct gcttttcgac ctggggatgg tgaaactctc catcaagtcc
    45361 acgatcctgg gggcaaacat cgtgggcggc ctgatcttcg gtatcggctg gggcctggtg
    45421 ggctattgcc cggccacggc catggcggcc ttgggcgagg ggcgctacga cagtctcttc
    45481 gggcttctgg gaatgctgct cggagcgggc ctcttcgccg aggcgtaccc gttcctcaag
    45541 gcgaccgtgc tcacctgggg aaacttcggc aagatcagca tcccggcggc cctgggtgtc
    45601 aaccactgga tcgtgatcgt catcctggtc gcggcctttg tcggactttt ctactggttc
    45661 gagaagaagg ggctatagga tctgccggtc ggcggaaccg tcgttgcaac cgttccgccg
    45721 accggaccgt tttatcgatt cttcggatcc ctcgggtctc ccttgtaacc cagggccttc
    45781 cagtcaaacc gtccgttctc gccgtggcaa tcgccgcact tcagcgagcg gtccctggaa
    45841 accaccatgt ggtgcacagg ccagatcatg gtggtttcca cgaagccgaa ccggccgctg
    45901 tacggctgtc cggcggcctt catcccgctg gcaatggcgc ggtcccagtc atacttctgc
    45961 caataggcgt cactgcccgg agggccgaaa acccgcacgt aggcgatggt gttgttccct
    46021 ctgtcgtagg cctgcttccc cctcatgatc ttgaacgggt agatcttgga atggggatcg
    46081 ttgcggccgc ccgtgacgtg cgtcagcttc accgctttgg tcggatcgat cttgtcgccg
    46141 gcatggtagc gttcaatgtt tccgttgaac cacaggtagg tgggcattac gtccttatcc
    46201 cacttgaagt ctcctttcat cttgtcgtag agcttcatgc cgtactggtc cttcgggata
    46261 tcctccgcct tgatgtcctt ccccgccgtg gaccagtccc accaggtctt ggtcggccgg
    46321 gccttggcga aggtagggat atggcagctc tggcaggcca ccctctcggt gtgcttgttc
    46381 accaggaagt tccggtgcac attgcccgtg tgacaatcga cgcagtcgat gtggctcggg
    46441 tgggtgtggg tgagggtgac ggaggccgct tcccccttga ggaagtggtc cttggcctga
    46501 tggcacgcct ggcaggtcat cttgctcttg ccccccatgt ggatgtcgat ctcgggggaa
    46561 ggatgggcca tggaggtgtc caagtctccg tgcttgacgt gatccccccc gccaccgtag
    46621 aagtggcagg ccccacaggt cgtgcggctc gtcgggccga cggactgggc cactttcaaa
    46681 aggtcggcgg gcggccatgg cgtgcccttg gggaactccc tggcttctcc cgcgtaggcc
    46741 gggtggcccg ccgcggtcgg aaatttcttg taggcaccgg tcgtgtcgtg gcagactagg
    46801 caatcgacgt tttccgcctt gctgaagtcg aaactctcat ccttgaagcc gtagccggcg
    46861 tggcaactgg tgcatcgcgg ccagttcccg ggcagcccga tgcagaagtt gttcagcgcg
    46921 ttcagcttgc cgatgttgac cgttttccct ttccatttct gttcttttgt ccaagtccag
    46981 tggacggttt tcatgaagtc ccgggtttcc ttctcatgac actcgataca cttttttgtg
    47041 acttcgggtc cgctcttgaa ggggcccttg ccgaccagtt cgacatggcc ggccaaggcc
    47101 gccgatgcca agacgattcc ggaaagcaat gagaatgcga cagcccatcg ggacgtcctc
    47161 ataaagaccc tccttccgtt catcgtttct cgagaagaat gaccctgctg ggtgttgatc
    47221 atagagaatc gactgaggga agtcaatccg aaaatatcag ctattccgga gtccttcatc
    47281 caaaccggcg gcgatcgtcg cccagaacgg gcgttccccc ggcggttttc tccggatttt
    47341 ctcccgaaag ccccacaccg tcttttgttc tctcgtcatg ctgaagggct ttttccgttt
    47401 gacatcccca tcccccatac cttaaggtct gaacgccaaa aaacaagcgt tgacccgaca
    47461 acgatccgac tttttgaaag gagtcaagcc atgaagaaat tcgccttgtt cgtattcaaa
    47521 ggagatcccc tgtgcttcat ccacgtgctc ttgaacgcca tggatatgaa agccaaaggg
    47581 catgaggcga aaatcatcct cgaaggcgcc gcggtcaaac tggtgcccga acttgtgaaa
    47641 gctaacaatc cgctgaatgg attgtggaaa aagagtctcg acgccggctt ggttgaaggc
    47701 gtctgcaaag cctgttcgaa caaactggga accctcgagg ccgccaaagc gcaagacttg
    47761 acccttcttg acgatatgtc cggtcatccc ggcatggcat cgtaccggga taagggatac
    47821 gaaatcattt ccttctgatc cgcggtgaag cttgcgtgtt cgggtttgac cccgtttgga
    47881 tctgcacggg caccccgccg gagggtatgg ccggcgggaa aagcggacga gatcacgtgc
    47941 gcgggccgag caggcgacag ggcaggccct tcaggttggg ggtcccgaac tcccggccca
    48001 gtttttccac ggacgtcagc aggttgacat tcgcttcccc ccagtcgtac tggcgggcgg
    48061 ggtcgccctc cgggaaccac catcccgggg aggcgcagat caccctggga tcgaggccgt
    48121 cggtgagccg ggcgcgctgg gtcatcgcac cgtgcctcgt ctcgatgaca accctctccc
    48181 cgtcgccgat gccgtggcgg gcggccgttt cggggtggat ctccaccgtc ggatggggcc
    48241 gtctttccct gagtcttcgt atccagcggt aggatgagtg caaaaaatag cggctcttgg
    48301 cgctgatcag gagcagcgga taatccggat cgtcctcctc gggaaatccc gaaaattcgg
    48361 gcagggccgt aagcccgaac ttccccgcgg tgctcaggcg cagctccacc ttccccgtcg
    48421 gcgtcgaaaa tcccttctct tcgtaggctc ggaagcggtc cggccccttg aggtatcccc
    48481 tctccacgaa ctgccggtag gtcagtcccg ccgggcggac gacgtcctcc agaaacgctt
    48541 cgtggtcctc tggccacagc tcgggggagg agacgcgttt tcccaattcg ttcaggattt
    48601 tcatgtccgg ccagcagccc tcgggcggct cgacgatctt gggacgggcg aagatcacgc
    48661 cgtggccgag accgtagtgc ccgatgtcgt tcatctcgta ctggtgggcg acgggcagga
    48721 cgatgtccgc catggccgcc gtcggcgtca tgaagagatc ggcaacggcc acgaagtcga
    48781 ggcgtttgaa ggtctcgaag gtgcgccggc tgtccgccca ggccaccatc ggattcgtac
    48841 acatcccgta gaaaccccgg acgggatagg gcacgtcctt aagaaccgcc tggcggaaga
    48901 aggccggggc gaccgtcatg agccgcggga tgatgccgtg gtgggcgccg atcatctcct
    48961 tgcgcttgtc cggaatgagg tcggcacgaa cgaacggtcc gagacccatg atcttcgggt
    49021 cccgggcgtt gacgttgccc ccggagacgt cgagattgcc gcagacggcc atgagacaga
    49081 caagcgcgcg gatcgcgtcg aaggcgttga cactgtgttc gacggcattg ccccactgga
    49141 gggccgccgg tttcgccgcg gcgtacatcc gggcggcccg gcggatctgc tcggcgggca
    49201 cccaggtgat ctccgccacc ctctcggggg gatacgcctt cacctgccgg acgagatctt
    49261 caaaaccgta agtgtacgtc tcgacaaagg ccttgtcgta cagggattct tcgcagatca
    49321 cgtggagcat cccgagggcc agggccggat ccgtaccggg cctgagcggg agccaaaggt
    49381 cggccttctc ggcaagctcg atccgccggg gatccacaac gacgagcttc gcgccgccct
    49441 tcacccgctc caggagctga acggcgatct gcccctcttc gtgggtggag gtgagattgc
    49501 ttccccagag caacatcagt tcggtcggat gatggaggtc cgccacgggg taaaacccgc
    49561 aggtgtgaac gcccgtgatc tcccgggggg catggcagac atcctgcgaa gcgacaacgt
    49621 tgggggatcc gaagaggttg gccaggcgga ttaaaacgaa atgttcgagc cccttcggca
    49681 tgccgacacc gaaacccacg gcccgggcgc cgtaggtctc ccggatgcgc cgcagattct
    49741 cggccgtctc ctccagggcc tgctcccagg aaatggcctg ccatttccct tcgcctcgcc
    49801 cgcccacccg tttcagcggt tgcttcaggc ggtcgggatg ggtgaggcgc tccgccgaca
    49861 cccgcccctt gaaacaggta tacccccggt tcaggtaccc gtccggatcg ccctcgatcc
    49921 ggacgatttc gttgccccgg accccgaccc ggagaccgca gcccccgtgg tccatgcgcg
    49981 cacagtgggt tttcacccag cgaagctctt ctgacatctc tctcgaacag tcggtatcca
    50041 cggcggtctc ggcccggatg ccaagccaat ccttgccctt gaacagccgg cccagggcgt
    50101 acccgggacg tcgacaggct gaccgggctg caggggccgc ctcaggcggc gaaggtcagt
    50161 ttcgtccccg acatgttcag gatgaactgc tccggcatgg tgttctccat atagaggatc
    50221 gccttccgcc cggtgcagtg cgtcgggatg acgtaatcgg gggcgagctt ccggagctcc
    50281 tccgtcgtcc ggccgacgat cggctcgaaa aacggtccgg acaggtggaa cccgcccatg
    50341 acgacgtgca ccttctcgat ccccgttacg ttctgcgcgt ggcggaccgt gttgacgacg
    50401 cccgaatggg cgcagccgga caggacgatc aggcccttcc ccttcagatt catgaccacc
    50461 gcggtgtcgt cctcgatcgg gtcccacttc tccacggcat cctcctggaa aaaggcgatc
    50521 gggaagccct tctcgaaatc cgtcacccgc tcgatctccc caagaaagcc gacatcgccg
    50581 tccaggagcg tgacgggcgc cttcgtctcg acgacgttca cgccggcctg ttccaggctt
    50641 tcccgggtaa tggtgggaaa gtcgaacttg agcccctcgc cgtacttcac gtagcggggg
    50701 gtcttgaaga tccccgggtg gacgtagaag ggcaggccct tcttcccgat catcgccgtg
    50761 agcgcggcaa agccgcccat gtggtcgctg tggccgtggg agagcgccag ggcctccacc
    50821 tgcgtgaggt cggcgttcag ggcgcgggcg ttataggcgg ccccctcctc ggaatagccg
    50881 aaatcgaaga gcagcgtgcg ggtcttcccg gcggcggttg tcttcacgag ggcggaaaat
    50941 ccgtgctccg ccaggatgga gttccggatc tgcccctcct tgatggccat cgcccgctgt
    51001 acgacggcgc tgttgtccat catcgtgatg tcgatggtgt tgtcctgcag ggtcaggatc
    51061 tccacccggt ccaaggcatt caaggcaatc ttcttcaaaa tgttggtctc ccttctttcg
    51121 ttgaaaatgg atgacattca aagcacgaac accacggccg ccagcacggt cgtgtagcct
    51181 ttgctcttca cgatgctcga ctgggtgatg ttcttcgtcg ccacgatctt gccgctgatc
    51241 cggaaaatct ccttcttttc gtcccagctc tcgtccacgt tgaacgcgat acccagggtc
    51301 gaggccagca tcgccgccgc cagatcctcc gcgtaatccc ccgcctgccg ttccgtctgg
    51361 ccgaaggcgt ggtgttcgct gatataaccg tacgcctgcc ggtccttcgg aatggccagg
    51421 cccacggacg ccgcgaggag ccgccgcggc tcgttgctgc agcagcggct catgacgcaa
    51481 tacgtgatgg cgcccggttt gatctcctgc aagccctgcg gccgggtgat gagccggcat
    51541 cccggcggca tgatgctcga cacctggacc aggttgcact tttcgattcc cgcgtcgcgg
    51601 agggccaact cgaaggagtg aagctcctcc cggtggctcc cgacgccctt ggtcagaaag
    51661 attctcttcg gcaccatggt tcgcttctcc actccctgcg tcttcgtcac cgcgccgcat
    51721 ccggccgcgc ccttcctacg ggaccgcgct tttcttgcca ttcctcgaca gcccaccgga
    51781 gcgtcaacga ggccagataa gcgcaatgtt cgtgatcctc cggcatgccc ccgagctcgg
    51841 ccagaatcgc gttttcgtcg atcttgagcg cctccgcgac ggttttcccc ttcgccaggg
    51901 ccaccgcggc atggcacgcc gcccggctga aaaaacatcc gctcgtcgtg aagcgggcct
    51961 ccgcgatcac cccgtcccga atcgccagga acatctcggt cgaatcgccg cagaaacctt
    52021 ccacccggcc gtgggcgtcc gggtcgtcca tgatgcccac ggcctcggcg ctatttccat
    52081 aatacagcaa cttctccccg taacgcggct gatcttcagt tctggccagg cccagcctct
    52141 cctcggcaga catcaactcc tcttccacgc aacctcactc ccgcatcggc gcgcacgggc
    52201 ggtctcaccc gtgcgtcgtc ttcatgattc gctctgaccg ttttcaatcg aacgccatct
    52261 tcgttttcca gacttcccag accttgccga cgagtcccgg accgggcttc agcgtgagtt
    52321 tccccggctt ccagcccgaa ggcatggctt ccgtgccctt gctcttgcgg acgtgctgga
    52381 aggcctggaa ctggcggatc gtttcgccca cgttgcgccc caccggcggc gtgagcacct
    52441 cgaatccctg gatgacgccg tcgggatcga tcaggaaccg tccccgggcc tccacgcccg
    52501 catcctccag ataaacgccg tagacggagc cgacccggcc gccgccgtcg gacagcatcg
    52561 ggaaaggcac gccgcccttg accatcctgg acagctcatg atcgttccac atcttgtgga
    52621 caaaaacgct gtccacgctc atggagagca cctcgacgcc cagctttttg aattcgggat
    52681 atttttcggc gaccgccgaa atttccgtcg ctcagacaaa ggtgaagtcc ccggggtaga
    52741 aacacaggct gacccatttc cccaggaatt cggacaactg aacggagatg aactttccct
    52801 tgtggtacgc cggcgccgta aaatcgggtg cctttttgcc gaccgtaatc atcgctttga
    52861 cctccttgat cattggctgc tgaggggaat ccttggacgt gtccgctttt tcgccgaccg
    52921 gaccgccggt cggccgggcg catcccgcct tgatttcctt tcccatacgc gatgtcctcc
    52981 cttccttcaa ttttgcctcg gcatcctcaa accggaaacg gggacaggcc ctgactccgg
    53041 aacatcttcg ggttcgtatt cccttgactt gaagaatcgt tccccgccgt tgacatacgt
    53101 cccgacccgc ccggcgtcag gaaaccggcc catagacgta tcgcaaggcg ccccgcccca
    53161 tcactcgtcc ccacatgacg cgcttccaca ccgcgcatcc cttccgcagc aggtccggcc
    53221 cgtttcttcg agggcgcacc cgccgccacg acccgccttg ccatgtccgg atcctttcag
    53281 gataaagccc gtcccgccgc tgatcaaccg gcgaacgccg cccccacacg ccggacattc
    53341 cacgagaggc gcctccgtga tggcctgccg gcgctcgaac ttcaaaccgc actcagcgca
    53401 ttcatattca tacgtgggca tgcttcaaac ccttttccac gcttctgctt ataatccggt
    53461 tcctgccgtt agggaagcgt tttccgttga agtccggctc agtgatcgca gacattcgga
    53521 cccgtggcga gggttccgtt catataggcc gtgaccacct tctccggcgt atcggccggg
    53581 gcgccgcaga cgacctgaat gccgttctcc gtaaaaagtc cctgggcgcg ggagcccatc
    53641 cccccggcga tgatgatccg ggcctctttt tcatgaagcc atttgggaag cagacccggc
    53701 tcgtgggggg gcggcgtcac atcctcccgc cggaggatct ttttcccgtc catgtccaca
    53761 tccagcaggg caaaaacctc gcaatggccg aaatgggcgc acaacagccc gtccgaaacg
    53821 gggacggcga tcttcgtcac ggcagcggcg gtccgcgacg ccatcggcaa gacgaccggc
    53881 gcgggcttct tgtccttcag attcaggatg gggtcgaggg ccttcatgaa cgcctgggcc
    53941 gtctccgttt tgttctggaa atagatgaac gggttgccgt cgtcggatgc ctcgacgatc
    54001 gcgggatcca gcggaattcg ccccagaaag ggaacgccca tttccctggc catggcctcc
    54061 ccgccgcccc gcttgaagac gtcgatgacc gtccggcagt tcggacagac cagtccgctc
    54121 atgttctcga tgaccccgat gacggggagc ttcaactgcc ggcagaaact gatggatttg
    54181 cgaacatccg aaagggccag gtcctggggc gtggtaacga tgacggcccc gtcgctgtcc
    54241 ggcagcagtt gcgccgcgga gagcggctcg tcgcccgtgc cggggggaaa gtcgatgatg
    54301 agatcgtcca gttcgcccca cgccacctcg gacaggaact gcttgatcgc ccccatcttc
    54361 atcgggccgc gccagatgac cgccgcatca cgctccggca tgatgaaacc gatggacacc
    54421 accttgatcc cgtagagata ctccaccgga acaatggatt ttccgtccga ccgcaattgc
    54481 ccctgctcca atttgagcag tttgggaatg ctcgggccgt gaaaatcgag gtccagaagt
    54541 cctaccttcc ggccctccag ggcgagggcc acggcaatgt tggccgcgac ggtgctcttc
    54601 cccacgccgc cctttccgga caggaccatg atcttgtgct tgatctggtt catccgctcc
    54661 gtcagggctt cctgctcccg ccgttctttc cgatcctggg cgtcggggcg ctgttgatgc
    54721 ccctgacggc ccgtttcctt gccttgacaa tccatgacgc ggtacccctt tccttgcatt
    54781 gcgggtccgg gaggaatccc tcctcaagga cccggttcat tcattgtccc tatggcgccc
    54841 ggcgcccgcg ccgcaccgcc tcttgctcat cgacctcctc atgccatccg cttctggggc
    54901 gatcgatccg gtggaacctg gccgcgtaag gcttgatatc caggagcggc gttccatcga
    54961 taaagtccgc cccgtcgagg tgcaggacat ggccctcacg gcgaagcagc gccacgagac
    55021 tcatgccgat ggcgttgggc cggccggggt ggcacgtggc aaagacgccc cgttccctgt
    55081 cctggaggaa cggcttcacg atcagtccgg ccggcgccgc aaggtgcagg tggtagatca
    55141 gaaagacatg tgaaaagctt tcgatgtcct gcagaccctc ggcatactcg ggaaacacct
    55201 ccgcacggcc ccaggccccc tgcgcataaa cgggctggat cggcgtctcc tccgcgaggg
    55261 tatgcccgct gcggaggagg ccgatcggcg taaaccggat cgcgccgctc atgcccagtg
    55321 gccctcgaca tcggcggccc gggcctccgt gagcaccccc gagcggtact ggtcaagggc
    55381 tgcggcgacc gttggggcgt cggaggtcag gatcacgaca ccggccgcac tcagaacgcg
    55441 aaacgccttc gggccgcagt ggccggtcac gacggccttc gccccggtcc gggccaccgc
    55501 ctccgcggat tgaatcccgg ccccctgcgc ggcgttcaga ttctgcttgt tctcgacgat
    55561 ttcaaaggtc cgaccctcca gatcgtaaac gagaaatctc ggggcccggc caaaacgtct
    55621 gtccaacggg gcctcgagat cggtccccga tgtcgtaaaa acaatcttca tggattctcc
    55681 ctttcacttt catccaggcg gtgtctgatg aaattcaatt cggactgcat tccggacccg
    55741 gcgccgcatc cttccgcgcg tccggtcacg acgcccattc ccatgggatc cgttcgattc
    55801 cctcccggca catcctacaa cccgttactc ccgaatgacc ggttatggcc ttgtcatgac
    55861 ggtcccgcac ttcgggcagt tgcggctgga gcagggcaca ccgcgctcat gcggctccgt
    55921 ttgcccgcac tgcgggcaaa cgcaacgtcc gacagtccct gccgcaaaag cggcggcggt
    55981 gcgacccgcc cttcgcgctc cccgaccctg tccatttcga ccggggccga tcggtcctgt
    56041 cccatctcct cttggcatcg cactttcctc gttcgccccc tgcccggccc ccgccgcatg
    56101 cattcgcgga agacagccca tccgcagatg attccggcaa cccggcaggg caaaccgaac
    56161 ctgccccagc cgatcgctcc gataggcccc aatcacctct tcaacgcggc cccgtacaaa
    56221 aggcaaaacc gtgatccctg acgattccag caggcgcttc aggggcatgg agatcgcccc
    56281 gcagatcagc agaccgaccc cgtttctctt gagccgcagg gccttccccg acccatccga
    56341 tccccccagc ttcattaggg ttcttcggcc gtcgccctcg agaacatcct cgataatcag
    56401 gatgtcctcg gccgcgtcaa agacggtcga gatggaatcc ttccagatgg ataaggcaat
    56461 cttcattcgg gttcccctaa cccggattca tcgtgcgtga cggacccgct tgaaaacctt
    56521 ctgcaagggg aacatcaaga tctgtgccat agaaccggca atcaccaaaa cacagcaatc
    56581 atcgatacaa ataccggatc ggggaaaagg cgggcaacca tgaagttgcg tgattgcaac
    56641 tgcgcggtac ggatgaggtg cgtgattgca actcaaggag caggctattc gggggtgacg
    56701 atccgcagag ccttgatctt ccggtagagg gtggtcttgt gaatgcccaa ctgcctcgcc
    56761 gtccgcagac gattccagtc gttcgcccgc agggcgttcg tcagatggat ggcctccatc
    56821 tcccgcaggg aaaggggttc cggccgagga tcgccgccgc ccatcttgaa cagggattcc
    56881 ggaaggtgga tcttctcgat ccgcccggcc ttgcaaagga tgaaggcccg ttcgatgata
    56941 ttgcccaact cccggatatt gccgggatag tcgtaggcca ttaggcaggc caaggcctcc
    57001 tcgctcagcc cctcgatctc cttctgctga aggacgttga accggcggat aaagtgatcc
    57061 gccaggaggg gcacatcctc catccgttcc cggaggggcg ggagggtgat cttaaagacg
    57121 ttgatccggt aaaagaggtc ttcccggaac agcccctgcc gaacccgttc ctcgagattt
    57181 ttgttcgtcg cggcgataat ccggacgtcg tgacggacgc tttcggtgga ccccaagggt
    57241 tcgtaggtct tctcctgaag gacgcgcagc aggcgaacct gcatggccgg ggtgatgtcc
    57301 ccgatttcat ccagaaacag cgttcccttt tccgccaggc ggaaccgtcc cgccttgtcc
    57361 cgcttcgcat ccgtgaaagc cccggcctta tagccgaaca actccgattc cagcagggtg
    57421 tcgggcaggg ccccgcagtt cacggcgaca aaggcctgtt ttttcctcgg actcaagttg
    57481 tgaacggccc gggcaaaaag ttcctttccg gtgccgcttt ccccttcgat gaggaccgta
    57541 ctgccgctgt cggcgacatc ggcgaggatg tcgaacagtt gaagcatccg gtggttcttg
    57601 gaaacgatgt cctcgaaggc gtatttccct tcgatggtct tccggaggtc ttcttcaaca
    57661 ctgatgtcgc ggaacgtctc cacggcgccg agaacggctc cggcggttcc tttgaggagg
    57721 gcggtggaaa tgctgacggg tcggcggcgg cccctggaat cgatgatata gaccgtccgg
    57781 ttgatgatcg gcgtacccga tttcatcgtc ttgcggaggc agcagtcctt ttcacagatg
    57841 ttcgccttca ggacatcctt gcactgctgt ccgatggcct cgtccctggg aacgccggtg
    57901 atcttttccg ccgcccggtt gaaagacgtg atccgccaat ccgagtcaac ggtaaaaacc
    57961 ccgtcggcaa tggaatccag gatgacgtcc tgtccattta caggatccac ggcaagcttg
    58021 ttccgggatg gatgcttctt catcttgtgg atcgggaatc ctctatcaat gttcgtccac
    58081 ctggttccga cacgagggca atgcaacctt taccgcgttc tccttgtcgg cggcaaattg
    58141 catcttgcgc cggacgcccg gcaggcagtg acgcatacgg tcctgaaggt aggcacatgc
    58201 gtgtcatctg tcaagcgata aaaacggcgg gggttttcct tgttgacatc tccagcccat
    58261 ttcttgtagt ttttaaggca taggacacgt tttgccgttg gaggattgac ggacgtcgtc
    58321 tccatgccgg aaggaggttc catgaccgca ccgctcatca tcgccatggc ccagggaaag
    58381 gaagtcgtcc tgctgccgaa catgaccaac cggcacgggc tcatcgccgg cgcgaccggc
    58441 acggggaaga ccgtcacact gcagaagatg gccgaatcct tcgcgtccat cggcgtcccc
    58501 gttttcgtgg ccgatatcaa aggggacctc tccggcatcg gcgcggccgg ccaggcctcc
    58561 gagaagatgc tcaagaggct ggaggccatc ggcatcaagg aattcgtccc gcgtgccaat
    58621 accgccgtct tctgggacgt gtacggcgaa gccggacacc cggtgcgcgc caccgtgtcc
    58681 gacatggggc cgcttctgct tgcgcggctc ctgaacctca acgaaacgca gtccggcgtg
    58741 ctgaccctgg tcttcaagat cgccgacgac aacggcctgc tcctgctcga catgaaggac
    58801 ctgcgggcca tgatccagtt cgtgggcgac aacgccaagc agttcaccac cgcatacgga
    58861 aacatctccg ccgcgtccgt cggcgccatt cagcgcgggc tgatgacgat cgaacagcag
    58921 ggcggcgaca aattcttcgg ggaaccgatg ctcgacatcg ccgacctgat gcagaccgac
    58981 ggcgagggcc gcggcgtgat caacgtgctg gcggcggaca agctctacaa caacccccgg
    59041 ctttacgcca ccttcctgct ctggatgctg gccgagctct tcgaacagct gcccgaggtg
    59101 ggagacgtgg agaagcccaa actggttttc ttcttcgacg aggcccacct gctgttcaac
    59161 gacgcgccga cggcgctgct ggaaaagatc gaacaggtgg tgcgcctcat ccgctccaag
    59221 ggcgtggggg tctacttcgt cacgcagaat cccctggaca tccccgaaac tgttctcgga
    59281 cagttgggaa accgcgtcca gcacgccctg cgcgccttca cgccgcgcga ccagaaggcg
    59341 gtccggacgg cggcggagac cctgcgcccc aatcccgcct tcgatgccgt cacggcgatc
    59401 actgaactgg gcgtcggcga ggccctgatc tcctttctcg acgagaaagg ccggccggcg
    59461 ccggtggagc gggcgaacgt catcgccccc ggttcgcgtc tcggcgtcct cgacacggcc
    59521 gagcgctcgg cggccatccg gaactccatc ctctacgggc actatgaaaa gatcctcgac
    59581 cgggaatcgg cctacgaaaa attgcgtgca tccgttcagg gcgccgcaac gcccgcgcaa
    59641 ccgccagtga cggcgggcga cccggaggcg gccgccccgg gcggcggagg cgtgctgggc
    59701 ggcggcatcg gcgacatcct gttcggacgg accggccctc gcggggggaa aaccgacggc
    59761 ctggttcaga ccgccgccaa gagcgtggcc cggacgatcg gctcgtccgt gggacgggaa
    59821 atcgtccgcg gcgtcctggg ctctcttctg gggggcggtc gacgccgttg aagacatcgg
    59881 gaattcaatc ggcttgcccc gtttcgggtg gggccctcag gaaatcgtcc gccaccgggt
    59941 gaacgatagc ggacatccca tgctgaccgt tctgttcacg atttttgcct cgtccttcgt
    60001 cgtcgcgctg tccggcgccc tgatgccggg gccgctcctg accgcgacga tcagcgagag
    60061 ctcgcggtac ggattcatca cggggccact gatgatcgcc ggacacgccc ttctggagct
    60121 tgcgctcgtc ggagccatcc tgcttgggct ggcgccgttt ttccaacgac cggaggtctt
    60181 cgtggccacg gccctcagcg ggtctgtgat tctcgcctgg atggccttcg ggatgttccg
    60241 ctccctgccc gcactcaccc tctcccggaa gcctgacccg cggcggcgga atcaccccct
    60301 gatcagcggc attctgatga gcgtggccaa cccgtactgg atcatctggt gggcgaccat
    60361 tggcctgggg tacatcctct actcctggcg gttcggcctc cggggtgtcg tttttttctt
    60421 tgccgggcat atcctcgcgg acctcgcctg gtattccctg gtctccgcta cggtggccgg
    60481 ggggcgacag tttctcaccg atcgccttta ccggggcctg atcgcggcct gcgccgcttt
    60541 tctcgtcgtg tttgccgggt atttcgcctt tgcggggctt ggaaagctga acgcgatcct
    60601 ctgatggggg tccactgacc gttgcgaggc gatacggggg atggccgtca agatgcggcg
    60661 acggtggccg gacggggacg gcggcggcga tgcctccgtt gttttgccgg ggtctcggcg
    60721 accggcgctt cctttccggt cgggggggcg tccccggaca ggaaggcgtc ccaccaggcc
    60781 acgaccgggc ggcgctccgc gctcgtttcc gccgccaggc ggaggtagcc caacccgtcc
    60841 gcaaactcgg gccgatggac catgcgcgcc gggcggcgcg ggggctggcg ttgcagcagg
    60901 ggctgaaggg caaggatggc gcgcagttga ctgccgatcc gcccgggcac gctgacggtc
    60961 cggcacagcg cctcgaaaaa aacggcgcac gcggcatcca gggcctgctg gcgtggaaca
    61021 ccgtcccggt gacgcgccaa ggccgcctct tccagatcgg gtccgaaaag agccgcgaag
    61081 cagagcggtg cgttcggggg cgtcccgttc cggcagagcc ggtcgagccc tgccagattg
    61141 gcctgcacga tggaccgccg gccgttttca tcccccccgc cgccgaggcc gggaaacagg
    61201 gcgggaccga gaccgctttc ttccagaaga gggaaaaccg gaccggcgga acccagcaga
    61261 aagagcttca gaacctcttc ataaagccgc gccgacgagg cgcgggtgat ggtgggagac
    61321 agttcgcaca ggacgtccca cgtcgccggt tcgatgacaa acccatggga agcggcaaag
    61381 cggaccgcgc ggagcatgcg taccgggtcc tccgtgaagc ggacgtaagg atcgccgatg
    61441 ggacggatga gccgctgttc gaggtcgacc agccccgtgg aatggtcgat cacggaaaaa
    61501 tcggcgatat tgtaggcgag cgcattgatg gtgaagtcac ggcgaagggc atcctcctcc
    61561 ggcgtgccga agacattgtc ccggagtacc atgccgtcct cgtccttgag atgacggggt
    61621 gggcggtgat cttcctccgt cttgcccgcc tccccctcat cgaccggggc ggccgagcgg
    61681 aaggtggaaa cctcgatgat ctcgtcctgg aagtggagat gggccagccg gaagcggcga
    61741 ccgacgaggc ggcagttgcg gaaaagccgc ttgagctgac ctggagcggc atcggtcgcg
    61801 atgtcgaagt ccttcggggt tcttcccagg aggaggtccc ggacgcagcc cccgaccagg
    61861 taggcgacga acccgttgtc ccggagccgg tagagcgtgc gcagggcgtt cgggctgatc
    61921 tgccttcggg agatgcggtg ctcctttctg gaaacgatgc gtggcggcat gggcggcacc
    61981 atagcacacc ccgtcgaaaa agcaacatcc aggcatgcgg ggcgacggcc ccgtccgcga
    62041 cagtcccgcc gcaccccgca ttcacgacga accgggattt ccgggtgcaa tttccgggtt
    62101 ggcaaagtct ccgagagctg atagggttcg ccaagtgacg ggcggacggt aagaacaacg
    62161 catcgcgagg ccctgccatg aatcccttcc agttgcacgc atgcctggcc gaggccttcc
    62221 gcaacgtgga acggcaccgg gacgcggccg atctcatccg ggagtattcc gtcaaccggc
    62281 ggaacatccg ggaggtggcg ctcgacgccg tggatttggg cgactgccgg caggtcctgg
    62341 atctgggcag cgggttcgga cccttcatcg acgtcctgaa gggacgcctc gcccccggga
    62401 cccgtgtgac gggggtggac gtcttcggcg actacggcgc accttttctg tccgcctgcc
    62461 gggatgccgg gtttcaggga aacttcctca aggaaggcgc atccgtgatc gagacctttc
    62521 ccgaacgcgc cttcgatctg atcgtctgca gctactccct ctatttcttc ccggaccggg
    62581 ttccggacat cagccggacg ctcagcgacg tgggaacgtt catcgcgatc acccacgacc
    62641 ggaacaacat gcgggagctc atctccaccg tcaaggcgat cctggccgag ctcggccacc
    62701 gcccggacga tcctctgccc gtggaaaaca tcctcggccg gttcacggcg gaaaacggac
    62761 gggcgctgct gcaaccctgg ttcggcgaga tccgcgcgat cgattacccc aacgccctgg
    62821 tgttcgaagc ggagagccaa ccgcagctcc tccgctattt ccgcttcaag agtccgttct
    62881 ttcttcacgg aaccgccttc accacggaaa cggtggcgcg acacctcgag gcccgcctcc
    62941 ggccgcggga ccggttcgtc ctgtccaaga acgatcggat ctttctctgt tcctcccccc
    63001 gcccgggaaa cgcctctcgg ccggacgggg gcaacaccag aaacccgtta ggggcgattc
    63061 aggcgccggt atgacgaaga catcgagaaa ttactgcccg cactgcggaa aggcgatggg
    63121 ccgggcacgt gaagaggggg tgctgcgcga cttctgcgcc acctgcatgc gtttttttta
    63181 cgacaacccc ctgcccgtcg tgaccgccct cgtggtgacg gaccgccggc tgctcctggt
    63241 ccggcgggga aaccggccct accggggacg ctggtgtctc cccatcggct tcgcggaggt
    63301 cggagagagc atcgaggagg ccacactccg ggaactgagg gaagaggccg ggatcgaggg
    63361 cagggtcacg ggcctcgtgg acgtggactc cgtccgcagc ggctattacg gcgacctgat
    63421 cgtcgtgacc ttcgaagtgg aggcccgggg gggaacgccg gcgccgggaa gcgatacgac
    63481 gcaggcccgc tattttccgc tgaacgccct gccgaagctg gccttctcgt ccaaccgcag
    63541 ggcggtcgaa acctacatcc ggcagatggc cgacacctgg gccatcgccg attccttttc
    63601 ccgggcgatg ggaaacgagg cggggcggga gaggaccgac ctcctgtccg acgccctgct
    63661 ccacgtcatc gagtggaatg ccgaacagat cgtgcatctg tggatcgccg acgtgacaac
    63721 gaaccgttcc acgcccgcgt accacgcggt cgatccggcg cgccttttcc ggcgggcgca
    63781 ccggatcgtc tcccagttcg gccgctggct ggggggggga tacgacgatg gagacatcat
    63841 gaaattttac atgcagctag gccgcgagcg gcgggaagag ggcttccccc tctcggaggt
    63901 cctgagcgcc ctcagcctga tccgcaagca catctggacc ttcgccctgt cccggggggt
    63961 ctggaaccgg accctggcca tctacatggc cctggaactc gaccgccgca tcgttatctt
    64021 tttcgacaag gcgaccttcc acgcgtcgcg gggctacgaa ggggcgacgg cctctacgta
    64081 gccgccctag ccggggccgg ggagacgccg acgactcgtg cctctccccg gcgccgaggc
    64141 ctctctttcc gaccgattac cgcttccccc aaaccagact gggctggcgc cagccggtca
    64201 ccggaacgaa gattttcttc gaggcgtccg cctgcaggat ccggtaatag tgggcgccct
    64261 tgcgcacatt cttgccgaac tggatctttc ccagtccgcc cgtgtcgaaa ttccggattt
    64321 tttcgacccg gtcgatcagg cgctcgcgcg tgaggttctt cccgcagtcc tccatcgcct
    64381 tggtcaacag gaggagcgga ttccacagga tgggaatcag gggctcgttc agcatgctct
    64441 ccagaccggc ctcggtgaag cccttctcga tctttttcca gccgggtccc ccctcgtggc
    64501 gcagaccgag gttcccgatg tggtaatgcc cgttggccag ggcgccggcc atgaaggtct
    64561 tgggatcgac ggacacgtaa tctccgaaga acacgggctt ccagccgatc ttgttggctt
    64621 ccatgagcag ggcggcgtag accggcgcat agccgacgaa aagacaatgg gtcacgcctt
    64681 ccgccttgag cttcatggcc tgggcgttca ggttcttcgt cccccgcttg tagggcgcct
    64741 ccacgacctt gaacccgtat tttttggccg ccatttccat tccgtccagg gtgtccttcc
    64801 cccactcgtc atcctgatag cagaggccga tcttcgggtt cttggccttc aggtccttga
    64861 agacgtaatc ggccatcagg atcccctggt cctggccgtt gcaggcatac cagaagaggt
    64921 agcgctgggg cgggtcgaac atcgtctgca cggccgcggc ggtcgtgtag acggggatct
    64981 tcttcgcctg aatgtcgggg aggatggccg tgcagggcgt ggagccgtag accgacacga
    65041 tcatgaacac cttgtgttga tcgacgagct tcgtgaacgc tgttttcgtc aggaccggat
    65101 cgtacttgtt gtcctccacg acgagcttga gctttcgccc gtggatgcct ccccgatcgt
    65161 tcacgtcctt catccacacc tgcaggacgc ccgtggccag tctgcccaga taggcggccg
    65221 gccctgacaa atcctggaag gtacctaaaa gaatggtgtc cttggtgacc cccacttcgg
    65281 cccgggcgat gcccccaccg agcgctccga acacgaaaca gatcatgaca atcccaacga
    65341 gcgtccgcaa ctttttcatg atgccctcct tttgctgccg tcgacaagat tcataaaccg
    65401 cttcgtcacc gtactaccga cccgtgacgt caagcgacct tcgcgcatcg acaaacatta
    65461 ccacttccgt cgcaacggag aacgcgaggc ccggttcccg ggacggcgac gtgctccgga
    65521 atgacgaaag gcagggtttt ccggtgtctt gctccctcaa cgactttcgc ccttttaccg
    65581 ctcaatacgt gtacggccaa gtcttccagt agcgcttgac gcggatccag atcccgaaca
    65641 gccccagcgg ttcgaagagg aggaaaaaga tgatgatgag gccgtacacc atgaccgaca
    65701 gcccgccgat ccggggcgcc aggtccggat aggcggacac cagcggaccg gagaccgcat
    65761 tgatcgcctc gggcaggagc accatgaaga tcgccccgag gaccgacccc aagacggtgc
    65821 ccatgccgcc gacgatgatc atggcgatgt acaggatcga ctggttgaat gtgaaatggt
    65881 ccggaccgat caggtgcaga agataagcgt acagggcgcc cgccacgccg gccatgaacg
    65941 aactgatcgc aaaggacatg atcttgtatt tggccatatg gatcccgatg atctcggccg
    66001 cgatgtcccg gtcgcggatg gccacgaagg cccgacccgt ccggctcatc aggagattct
    66061 tcgtgaagac cgccgccgcg acggcgagca ccaggacgac gtaataatag gactggtaag
    66121 agttcagagg gaatccgaac agatccggag tcggcagggc gatgcccttg tcggactggg
    66181 tgagactctc ccagtggtag atgacgtgct cgacgatgaa ctgaaacgcc agggtggtga
    66241 cgacgagata aagccccttc aggcgcaggg cgggaatccc gacgatcaat cccgcgagcg
    66301 ccgccgccag gccggccagc ggcagcgtgg cgatcagcgg aagacccgtc cggacagcca
    66361 ggatcgccgc cgtgtaggcc ccgataccca tgaaggccgc atgccccatg gacagcagcc
    66421 ccgtgcagcc cgtcaacagg ttcaacccca gggcgctgat gatcgagacg ccgcagatgc
    66481 acgcgacgaa aaccccgtat gaccccatga acaggggaaa ggtcagtaga aacgcaccca
    66541 gcagcacgag cgagatcctg gccgccggac tcgggaaaat ccggagatcc tgcggatagg
    66601 accggatggt gaatcgcgtc acagccatgt cggttttttc tccccgcttc cggcgccccg
    66661 tcagacgcgc tcgatttcct tcgtgccgaa cagaccgtag ggacggatca tcatgatgat
    66721 cagggccagg acgtaggcgg taatttcatt gatgccgctc ccaagataat cggccagata
    66781 gaaaccggcc aggttttccg caacgccgat gatgagcccc ccgaggatcg ccccgtgcac
    66841 gctgtccagt ccccccagga tcagggcggg cagggccttg agtcccgtgt ggctcatgat
    66901 gggatcgagg taggtcaggc tcgccatgaa gacgccggcc accgtcgcca caagggaagc
    66961 gatcacccaa gaggccgtga acaccccctt cacgttgatc cccatcagaa acgccgccga
    67021 ggtgtcttcg gccgtcgccc gcatgctgat gccggactgg gagtacttga aaaaaaggaa
    67081 gaagccgacg aggaggatca tcgtcgcgac gatggtgtaa atctcggcct ggttgatggc
    67141 cacgccgaag atcgtcaggg ggtccttggg aaagggcgcc gggaaattgt actcctcata
    67201 cccccagacc agccccgtca tgccgcggat gacgttggcc agccccaggg tcaccatgat
    67261 caccgaaagc accggctctc cgaccagggg acggaccacc agcatctcca gcagtgccgt
    67321 gatgagggcc atcaggacca gggcggcgag aaacgccccg ataaacggca gcttcaggac
    67381 cgtgatgaag gcaaaggtga gataggcgcc cagcatcatc agctccggct gggcgaagtt
    67441 gaagatgtcg gtcgccttga agatgagaac gaatccgagg gcgatgcagg cgtagacact
    67501 cccccaggcc agacccgtga tcgtgagctg tatcaggatg ttggacgcca tgttcctccc
    67561 ctttcaccgc cggggcgcga tcggccggcc cggggttaat tgcggtacat ctcctcgatg
    67621 atctctccta acttttcgta gagggtcttg cgacgcagct tcagcgtcgc cgtcagttca
    67681 ttgtcatcgt gatccagctc cttctccagc agacggaact tcttgatcgt ttccacctgc
    67741 gagaaatccc ggttggcctc atggacctcc tgctgaacca gctcgcggac ctcctgaagc
    67801 tgggccaggc tcttgaaggt cgtatacgcc agtccccggt cctgggccca tttggccaca
    67861 ttgtcgtact cgatctggat cagcgccgtc aggtagggtt tcgcgtcccc gatgacgatc
    67921 gcctcgttga tgtaggggct gcacttcagg cgattctcga tctcggaggg ggcgatgttc
    67981 ttgccgccgg aagtgatgat gatctccttc ttccggtccg tgatcttgag ccgtccctcc
    68041 ggatcgagtt cccccacatc cccggtgtgc agccagccgt ccaccaccgt ccgcgccgtc
    68101 gcctccggat ccttccagta gccgacaaaa acgtggttgc acttgagcag gatctcaccg
    68161 tcctcggcga tccggatttc aaccccgcgg atcggctttc ctacggtccc cagcttcacg
    68221 tcccccggca ggttggctgt gccgatgccc gtttcttcgg tcatccccca cccctcgagc
    68281 accggaacac ccagagaatg gaagaagagc agcacctcgg gcgatatagg cgccgccccg
    68341 ctcaaggcca gccgggcgcg cagaagcccc agctccttcc gcagatgacg gaagagcagc
    68401 cgttccccca ggccgtgcga gagcctgagc gaaaagggaa tcggcccgcg ggacaggcgg
    68461 gccagcgcca tctcccgtcc gacgggcatg aagaggcggt aggcggtcgc cttcgcccag
    68521 gtggagtcct tcatcttgat gacgatcccg gccatcatct tttcccagat cctcggcacg
    68581 gcgagataaa acgtcggaaa gatttcccgc atgtcctggg tgacggtatc gacgctctcg
    68641 gcgaaattga ccgtcgcacc ggaatagatg ggcagaaaaa cggacatcat gcgctccgcc
    68701 acatggcaca ggggcaggta cgaaacgatc tcgtccgtgt cgtattgggg cagggtctga
    68761 ctctgggcct tgagaaactg ggccacgttg tcgtggctga gcatcgcccc cttcggggga
    68821 cccgtcgtac cggaggtgta gaccatgatg gcgacatcct ccgggcggac ctgatcgacg
    68881 gtgcgccagt accgctccgg gtctgccgcc tcgatccggc cgccgatccg ctccacctcc
    68941 tcgaaggaga caatcatcgg atccgtgccg cggcgcaggc ccttcatgtc gatgacgatg
    69001 atctttctca tgaagggcag tgcttcccgg acctccagga ccttgtccac ctgctcctgg
    69061 tcgtcgcaga caacgaagac cgattcggaa tgaccgatga cgtactgggc ctcccgggaa
    69121 ggattcgtgg ggtaaacgcc cacggccacc ccgcgggcgc tctgggtggc cagatccgcg
    69181 taaagccact ccggaaggtt ttcgccgaag atgctcacat gatcgccggg cataagaccg
    69241 aggtccatca gccccaggca gaagtgccgc acatgccggc tgtaatcgtt ccaggaaatc
    69301 tcctgccaga tgccgaattc cttctcccgc agggcgaccc ggtcgccgta ctttctgacc
    69361 cgggcgagaa agatgtgggg aacggtatcc tctctcatcc cgccccctcc ttcttgagaa
    69421 gcgtggtggc gtcatccccg aggtaggcct tctggacctc cgggttcctc tgcacctcgc
    69481 cggtcgtccc ctcggcgatc ttgcggccga aatcgaggac gcagatccgc ttggagatgt
    69541 ccatcaccac gtccatgtca tgctcgatca tcacgatcgt ggtccccaac tcctcgtgaa
    69601 tgtccagaat gaagcgggcg atgtcctccg tctcctcctg gttcatcccg gccatcggct
    69661 cgtccagcag gagaaggcgc ggctcctgga ccagggcgcg ccccagttcc acccgtttct
    69721 gaaccccata gggcaggctg gacacgaggt gcttccgcca gcgttccatt tcgagaaaat
    69781 cgatgatctc ctcgaccttt cgccggtgga cggcctcctc ggccgcccga tagccgaaga
    69841 cggcgttgtg ccagaatccg tgccggaaat ggtggtggcg gcccagcatc atgttgtcga
    69901 tcacgttcat ccgggcgaac agctccacgt tctggaaggt cctggaaatg cccagcttgg
    69961 cgatctgatc gggccgccag tgcgcgaggt cccggtccgc gaagaggatg ttcccaccgt
    70021 ccggccggta gaagccgctg atgcagttca acaggcaggt cttgccggca ccgttgggac
    70081 cgatgacggc gacgagctcg ccctcctcga cattcaggga aacgcgatcc agggcaacga
    70141 tgcctttgaa atggaggctg atctcgcgga tctccagaaa tcccatgggc ctcctttagg
    70201 aaagccagcg cttgcggcgt ttgtaatgct tgacttcggc aaagctccgc cgttcctttt
    70261 cgtccttcat gcccaggtag aactccttca cgtcctcgtt ttccatcagc ttcccgctcg
    70321 ggccgtccaa aacgatcttg ccgttctcca tgatgtaccc gtattcagcc accgcgaggg
    70381 ccatgaaggc attctgctcc acgaggagga tcgagacccg ctcctcccgg ttgatgcgcc
    70441 ggataatgtc gaagatttcc gtgacgagca ggggggcgag gcccagggac ggctcatcca
    70501 gcatgatcag ggacggccgg gccatcaggg cccgcgcaat ggccagcatc tgctgctcgc
    70561 cgccgctcag gaaccccgcc tggatcttca gccgctggcg gaggggggga aagtagccca
    70621 gaacacgctc gtaatcgcgg gcgatctcgg ccttgccgtc tcggcgcgtg tgggcgccga
    70681 cccggatgtt ctcgtcgacc gtgagttcgg caaagatgcg ccgcccttcg ggaacctggg
    70741 tgatccccat ctgcacgatt tcctgcggat ccattttatg gatggggtga ccgtcaaact
    70801 cgatcactcc cttcttgatt tcgccgtcct cgtaatcgag aagccccgag atggccttga
    70861 gggtggtcga cttccccgcc ccgttggatc cgagaaaggc cacgatcgcc ccctcaggaa
    70921 cctccaggga aatccccctc aggaccagga tgatgtcgtt gtagatgact tcgaggttgt
    70981 tcagcctgag cgatcccacg tccggagaaa cctccttcgc cgcaacggac ggccaaagac
    71041 aggcccacac ttccgagaaa cctctaaacg gctacaacat ctttacattt ctggagactt
    71101 ccccccgtga atgtcggcgg aaagcaccgt caggaaaaaa cgggaggaaa tgacaggccc
    71161 gaacagacgg ggggccggac aaacgatcgg cctgaacacc cccgccgtgg gcgcccgggt
    71221 gaccggtccg gcggggcgcc ggcagcgtcc ctgacccaat catcccggca cgaacaacgc
    71281 gcgaacggtt cacggataac caaaattcat cgggatgtca atacaaaaaa ggggcctgat
    71341 tcgcgggcgt gggtcagtgg atgtcatccg atgcgccggt tgaccgatca tccccttacg
    71401 tccgtcccac ggtcgtcccg cggtcgtccg gccggtgtca tcgtcatcgg gaccctgacc
    71461 gccgcatcgt cgtctcttcc gagaagacaa cgccccctgc gacacggggc tacgacgggg
    71521 cggcggcggt ctcttcgaac acataacgga ccgtcagctc cagatccgga cgcagggaat
    71581 tgtacacgga gcagtaggtc tgccgcgaca gggcaatggc gcgttcgacc cgccgcgcgt
    71641 ccaggttctg accggccatg tggagcgcca tctcgacgct ccggaagtac tggggcggtt
    71701 cagggttccg ctgaccggtg acatcgaccc ggaaagcggt gagcgccacg cgcatctttt
    71761 tcaggatcga cacgatgtcg atggccatgc agccggcgag cgacagcaac agggcctccg
    71821 tcggattgca gccccactgg gattcggcgt cgaactcgat ctcgtagccc tgcggcgtcg
    71881 tccccgcaaa gaccagctcc cggttccagg cgagggtcgc cttcaccccg ggggtaatcc
    71941 cggccttgta cccctgaagc gcatccctca attcgccctt gtcctgttcc cccatgccga
    72001 tcctcctgac cccgtgatat ccctttataa ccccgaacgg acgagaattc aagacagggg
    72061 gagcggcgga catgtgaaaa aaatctggaa aaattccccg gtattgttta aagaaagccg
    72121 ttaatcgaaa ggccagggac acaccgtccg aaggagaacc gtcatgagtc aggaaaaaaa
    72181 cgtctatcag ttccgcgggg gctcgaaata cgtcctcgac gaggagctcg cccggatcgt
    72241 caatgtctcc atggcgctgg agatgccgct cctgctcaag ggagagccgg gcaccggcaa
    72301 gacgatgctc gcccacgcca tcgcggaaag cctaaaaatg ccgctgatcg ttctgaacgt
    72361 caaatccagc atgaagctga tcgacgccct ctaccagtac gacaccctca cgcgcctgaa
    72421 cgacagtcgc ttcggcgact ccaaacggga cgtgagcaac atcgaggact acatcaagat
    72481 gggcaagatc ggccaggcct tcaccgccga aacgcgcacc atcctcctga tcgatgaaat
    72541 cgacaaggcc gacacggact tccaggacga catgctggac gtcctggacc agatgcagtt
    72601 cgacatcatc gagatcgaca agacgatgac cgcccggcat cgtccggtca tcgtcatcac
    72661 ctcgaacgcc aagaaggacc tctccgaccc cttcctcggc cgctgcaact tccatcacat
    72721 cgcctttccc gacccggaga tgatgcgccg gatcgtcaac gtccacttcc ccaacttgag
    72781 cgacgatatg ctggatgcct gcatcgccgc cttctaccgc atccgggaat tcaaggggat
    72841 cgaaaagaag cccgccacgc gcgaactgat caactggatc cgggcactca agaccgaccc
    72901 ggatttccgg gtgcgcgccc tggagaaggg cgaaacgccg tttctgggaa tcctgttcaa
    72961 gaaaagcaac gacttgcaga tggccgccca gcaggcggcc cggtccagtc tgcggtaggg
    73021 gcgaaacgtg ttcgtcgatt tcttctacac cttgcgggac cagggcatcc ccgtcacccc
    73081 gacctctttc ctgaggctgc acaaggcgtg cagcatgggg ctcgtgaact ccctcgagga
    73141 tttctacacg accgcccggt gcgtcctcgt caagagcgag cgctacttcg acctctacga
    73201 ccagatcttc tcccattact ttcaggggat gaccctcgag acctgggacg accaggagat
    73261 cacggccgcc gcgcgggccc tgctcgatga gtggctgcag aaccccaagg agctggccga
    73321 cgccctcggg ctcgacgccg aaaagctcaa ggcgatgacg ccggaggagc tcatcaagta
    73381 ctttttcgac cgcctgaagg agcagaagga ggcccatcac gggggaaacc gctggatcgg
    73441 gacgggcggc acgtcgccgg tcggccattc cggccatcac cccggcggga tgcgcgtcgg
    73501 cggccggtcc ggaaacaaat cggccatcaa ggtggccttg gagcgccgct acaaggacta
    73561 ctcccaggaa ggccccatca cggaatccca gctcagcgag gccctgaagc gcctgcgccg
    73621 gatgatcccc cacggtccga aggacgtggt caacgtggag aagacgatct acgagaccat
    73681 gcgcaacggc ggtgagatcg acatcgtctt cgaccggagc ctgagggacc gcctcaaggt
    73741 gatcctcatg atcgacaacg gcggctggtc gatggatccc tacatcgaaa cggtccagac
    73801 cctgttcaac tacgcgcgct cccagttcaa ggacctcaag atctactact tccacaatac
    73861 cgtttactcg aaagtctggg ccgacccgca gcggcggctg aaacccgagc cgctggccga
    73921 gtttgtccgc cgggacccgg agacgcggct gttcatcgtg ggcgacgcca gcatggcccc
    73981 ctacgaactc atgcacgtca acggatcgat ctacgtggac gaccggcagt cgctcgccag
    74041 catggaccgg ctcagggtcc tgcagaagac cttccggcac agcgtctgga tcaacccgca
    74101 gccggaggag gagtggagct acacctggac ggtcaacgcg attgcggagg tgttccccat
    74161 gtacccgctg accctggacg gcctggaccg ggccgtgcag tttctcgtca agaagaactg
    74221 aaggggcggc tgcccgttga acggaagagt cgagcgcaca tgcgaggagg acaaacgatg
    74281 aaaacgaaaa tggggatcgg tctcgtggtc ctgacggcgc tgcttttctc cctctgcccg
    74341 gagctgccgg ccgtcccgcc gccggccgtg ggcggaacgc tccccaacgt agagctgcca
    74401 ggaccgaagg acggctccga ccggcgttat ctgggcctgt ccggctcctt caaaatccct
    74461 gaaatcaagg cccaggcggt catcgtggaa atcttcagca tgtattgccc ctactgccag
    74521 aaggaggccc cggtcgtcaa tcagctcttc gcccggatcg agggaaaccc cgcgctcaag
    74581 gggaaaatca agatgctcgg gatcggcatg ggcaactccc ccttcgaggt gggcgtcttc
    74641 aagaagaaat acaaggtggt ttttccgctc ttccccgacc ccgattttgt catccacaag
    74701 cggttgggcg aggtgagaac gccgtacttc atcgtgatca agatccatcc cgacggcagc
    74761 caccgggtga tctactcgaa gctcggggcg ttcgaggggg tggacgcgtt cctcgccgcc
    74821 attgtccggg atgcggggat caaataagcg cagacatgcg gtcattccct tggggaggag
    74881 cgtggcaatg aagaaaatag gtccgatcgg ggcggtgtgt ttcttgggtg catttctgct
    74941 gctggcggcc ggagtgcccg ttgatgcggc ctccgaggcc ttcaaggaca acatctacga
    75001 caccggtccg ctcaaaccca gggacagcgt tctcaaggtt aaggtcggcc ggttcgcccc
    75061 ggatttcacc ctgagggccg ttgcgggcgg tcctgtctcc ctccggcagt tccggggaaa
    75121 gaagaacgtg gtgctctcgt tcgtgccggc ggcctggacg gcggtctgct ccgatcaatg
    75181 gcccggttac aacatcgtca aggagctctt cgacgagcac gacgccgtcc tgctgggcat
    75241 caccgcggac aatatcccca cgctttattc ctggacgaac cagatgggaa cgctgtggtt
    75301 cccggtcctc tccgatttct ggccccacgg aaagacggcg gcgcggtacg gtgtgctgcg
    75361 ctcggacggc accaccgagc gggccctctt cgtcatcgac aagcggggcg tcatccgcta
    75421 tatcgacgtt cacgacatca accagcgccc ttcgctcgag gtcctcatgg gcgaactgga
    75481 aaagctccct tagcgccttc ctcccggccc tgccgtcttc gctgaccggg cgatcgcccg
    75541 gtcagcgaag gagataagac agggtgatcc cgaggtacgt tcccaccacc cagccgatga
    75601 tcccggtgat gaccccggaa agcacgattt ccttgttctt cagcgccgtg gcgaccatgg
    75661 gcacgagcgg cggggagcag atgcccgcga cggaggtgat gatcaccgtg tcggcatcga
    75721 tcctgaacaa cgcggccagg gcggtgtgca gcaccaggca ggccaggacc gtgaaggtca
    75781 cgtaggcgat catgatcggg gcggtggaga ccaggcggtt gaagtccgcc atggaactca
    75841 ccacgaggca gaagatcagg atgaagtagt tccccgcctg gtaggtcatc gcgatcttcc
    75901 ggatggtcgg cacgaatgaa aaaacgatgc ccagggtgga gatggtcaga atcgccgtca
    75961 caaaggcgac atcctccgtg aagatcagaa aggacaggcc gccggcaaca aagatcagca
    76021 ctgctagtcc cagagcgccc agcagcggca aaaaggtttc cttcgcgaag atcccggcgt
    76081 acgaatcgaa ttcaaactcc gtttccggcg ccgaggtttc ggcgggtctg aaggcgggca
    76141 ggaagcggag aaagaggcgt tgcccgacgg tcatcaggat gagcagatag acggaagaca
    76201 cgatcacgtc ggaagtgtgg gcggccaggt acagggtctg atcggtcctg agcgccgtgg
    76261 cgatggcgcc gagattcacc gtcccgcccg tgtacacccc cacgaacatg ccggccaatt
    76321 tccaggtctc ttcccccacc cagccgcgaa atacgaaaaa ccccagggtc gcgacgatca
    76381 ggatggatag aatctgcagc atgaaggaca ggagcgattt ccccgcgagc ctgctccagc
    76441 ggcggacgtc aatggaaaaa aacatcagcg gcagcgccag actgatggtc accagcatca
    76501 ggggattttg cagggcggcg atcccggcgg gcatcatgcc gatgttgccg aggatgattc
    76561 cggcgatata gcagatcacg accgctccga gcttgttgaa gagggcatat cgcccggcca
    76621 gccagatcgc gacaacggga aacagaaggt aaaagatgat gagtaaagcg gccgccataa
    76681 gacctccttc gatcatgacc gtcacgtcgt cagaaaatgc cttccggaaa ttggtttccg
    76741 ccaaccgttt gcaaaagaac gccggttccg gcggtcgccg gaagcaaccc ggatcggaca
    76801 atcacaacca gacatgatca tcgagaagaa ctgcgcagga tggcctcgat catggcatcg
    76861 ctcattttca tcacatcgct gaccaggctg gcctccccca gggagaggga tctatcggtg
    76921 aagatgaccc tcctgtgatc gatgctgatc accttgtagt ttatgatgat cagggtcccc
    76981 cgcttttcaa tgctgcccgt aatgacgata ttcagtccca gccgggtgcc gatgtggatc
    77041 acgtcctcga tccggtccgt ctggtgaaga tcgttgacca ccaggaacgt ttccagatct
    77101 ttccggtcca gcaggtcgaa cgatgcctgc attttcgggg cattggtcag cgtaccgagc
    77161 accttgaccg cgtactggga agcatccata cttgaggacg taaaatgaaa aacagccacg
    77221 ctgatttgag aggggattag aggcggagct ttgaccggct gactttcaac cggcgctgtt
    77281 gtctcgggac cggcaacggg cttgggcctg gtgagaagca ccggtcccga ctccaccgca
    77341 aacccgtaac gggcggaaac cacgtacccg ttgcggcgat agtcgtaata agccctcacc
    77401 tcgtatttcc ccggcggggg ggcgtcaaag gtcaggacac cctgacttgt cccctccggc
    77461 atgtaccggt agtctcctgc gtccgtgtcc ggggaccccg ccggcacgat gcagatccaa
    77521 tcccccccat cgcccggagc accggaaaaa tgaacctgga tcttctcacc ggaacgatag
    77581 cggtccttgt cggatgtgac cctgggttcg ccgccggcag gacttggcgg gacgaagagg
    77641 gtcaccatga tcaaaaggac catggcggcg accggccttg tcttcagact catcaggtgc
    77701 atctgtttcc tcctccgaag ccgggatcca cccgtttctc cccatcgaac ggcgatgcat
    77761 gatgcccccc ggatcccggt ctccggcaag gaaccgcccg gaacgatcag tccagacgga
    77821 tccgccggta ggtcccctcc ccgccgtcgt agagggcgca ttcgccgtcc tccaccgccc
    77881 cgacgacggc gatgtagcgc tcgtctgccc tcatcgggac cggaaggtcc ggggggatcg
    77941 cctcccggct caccctcccg gcccgccaac ggaacaggac cggcgtgtgg gcgtggccgc
    78001 agaagaggat gcggtgcgcc gtcgactgaa aaaccggaat ggccttcgcc gtcgtgccgt
    78061 cgtcgaccgg ctcgtagaaa gaccggaggg aatcgtaagg gagggaatgg gcaaagcaga
    78121 ggccgtcggc aaccagggtc atcggcagcg atttgagaaa ggcccggagc tggacttccg
    78181 ccggatccgg accggggggg cgctccttca ggatcgtctc caccagaaaa tcgttgtttc
    78241 ccttgaccgt catcatccgg taccgtcgca gggcctgcat gaccctcatc caggaggatt
    78301 cctgcaccgt gtcgcagaag tcgcccagat gaatcaggcg ctccgccccg gcggccagga
    78361 gcgcgtccgc cgcctgctcc gtccgtccga gatcgccgtg actgtcggcc agaaggccga
    78421 tccgccgcgc ctcggccacc gatgtctcca tgaaatccgt tctcctgatg aagcaatcag
    78481 ggttttcgta tagcacagat ttccggcggc ggaaaatgct ttctcccatc gtccgggggc
    78541 gcccccttga cggccggaag tttcttgacg tatacagacc gaacaacctc aggtggagga
    78601 ggatcctcca tcccgtggag acggaagcca tgaaacgccc gcgcgccctc gtgaacgggg
    78661 caaccgtgac cgccgcgctt ctcctgatcc tcgcactggc ggcctgcgcc ccgtacaaag
    78721 cctggctcaa ggggcaggcg gaaaaccacg cgacgcgaca catcgcgttc tggggagagg
    78781 cgtggcaaag cgcccccctg agcgcgaggc tcgccccggc ccccccggcc ctgatcgaga
    78841 aaatccgcct cgaaaaccgc ctctacggat tcccggagcg ccccgaaccc gtcacgccag
    78901 aacccgtctt cacggaggcg gtccggcgga tcggcgacct cctgccggag cgcgtccgaa
    78961 gcctggccga acagcggatc atcggcatct atctcgtcaa cggactgggg ggaacgggct
    79021 acgccgaggc gattctgaac gcggagggga aggagcgcta cgccgtcatc gtcctggacc
    79081 gcgatgtcct cctgacacgg cgggcgaatg cctgggcgag ttggaaggag aacagctttt
    79141 tccgtccggc gaccggcatg gagaccgcac tcgagctgat cctggagacg gacgaggagg
    79201 actccgtcgt caacgccatc gtcttcatcc tcctccacga gatgggtcat gccctgggaa
    79261 tggcgagcgg cgtccacccc tcgtggaacg gccccgcgga ggtctccgac gcctatccct
    79321 ttacggccct gtcctggcgc cggcagggaa gcgacgtcgt cagtcgcgcg gacgcggcgt
    79381 tcccccggcg ttccgccctc aagccctacg ccttcgccgg ggcaaggctg tcgctggacg
    79441 aggcgccgga gatctaccgc gcgcttcagg cccactccga ctttccgtcc gcccaggcgg
    79501 cggtgaccct ctgggaggat tttgcggaga gcttcgcgac gtacctgcac gtcgtccgcc
    79561 aggacaagtc ctacgagatc cgcatccgtc gccccgaccg gccggagcag gccttcccct
    79621 cgtgctggcg cgaggcgcgc tgcgacaaca agaaggcctt catgcgccgc tggttcgaaa
    79681 accccgtatc cccggcgctg ccggcgccgc cttaggcgac ggcgcaaaag tgtccgcatg
    79741 cagacccacc gaaaccctga gccgcaaggc gcagcggctg ggaggccgat cggcgatgga
    79801 tgggggaaat aaagccgatg gcggcttttc gaccgacggt cagcggagca tctccacgaa
    79861 ggcctccacc cgcgtccgga cctgctggcg ggagccgtcg ctgtagtcgt gctccaggta
    79921 gaaattccgc acgcccagac cgttgagata gtcccggacg gcgggaatct cgtagccgtg
    79981 ggtgtcgcag tacatgacgc tctggaggat gacgccgtcc acccgccagt cgcggatcag
    80041 gggggcgaga taaccgaagc gggcctccag atcctcccgg tagggtctgc cacccgcgtc
    80101 gtccgcttgg cggtacgtcc ggggacagcg gatatcggcg aggtagtgcc gggccagcgc
    80161 cgtcagggga tcgccctccg tctccacatc ggtcgagaag gcccgcgtcc cgacgcagat
    80221 gtcgtccgtg acgacctcgg cgccggccgc cgcgacgagc tcgaacaggg acgtgtcgtc
    80281 gatcacgctg ccccagacga ggagccgggg cgccccggac ggcgccggga gcggccgctg
    80341 acggacctcg gcgatgaccg ccgccaagag gtcgttcccc tcctcgaccg gaaggccggt
    80401 gaccgcgagg gcggctcgct gcgcctcgac cccggtcagg cggggcggga ccgtccggcg
    80461 caggtcgtag agatcccgca gaagggcccg ctgccggttg tgcgccgtga tcgccgcgcg
    80521 cagggccgtc ccgtcgaggc gccggccgct caacccctcc aggtccttca gcaggatctc
    80581 gatctgggtg cggaaaaaat ccagggccgg cgcgtgcacc gtgtggggaa cgtccaggaa
    80641 atggacgaac gcgggcgccg tcacataatc ccgccacaga tgtacggaca tggagccgac
    80701 atcgcagatg tgcgccccga ccaggccgtc gagaaaggcg tattgcccct tcatgcccag
    80761 gtcgatcagg ctccggagaa acgggcagac cgcgggcatc agaacacggt tgccctccgt
    80821 caccggttgc cgccggtcgc cgaagacgcg gaaggggaca aaccccaggg ccgtcatcag
    80881 ctccacgggg ggatagaggc acaggtagcc catgaggcgc gcgccgccct ccttcaactc
    80941 ccgcgcccgc agggcccgat cccgatagag ccgctccgcg cgcgccaatc cgttgtgctc
    81001 gtccgtcatc gtcatgtcct cgtcgatttc ctgatcggcg ctccgcaacc gcgcccccgg
    81061 tgcatcggat gcccggtcgc acccgggcgt cgatgccccc gcctcttccg atcctaccag
    81121 tcggatcccg cctgccgccg ctcttcgcgg taatggtcca tcgtcgcctc gaaggcctcc
    81181 gcctggatca gcgtccgctc cgggttgaac agacggatgt ccacgatgtc gccctcggcc
    81241 aggagcgacg gcacccgcaa tttccggatg aggacgtcct gcgtcgcctg gaggtgggca
    81301 ctcgccgtcc ggcaggtcag caggggatgg aggaaggccc cgtcgcagcg gtacgtccgc
    81361 gccgaatcaa gatagggagc gacgaaggca taggagacgc cgtccgagaa ggcctgccga
    81421 tggcgcaggt gaaaaaaacg catgcagatg cgggcgatcc gctcgagggg atccgggacg
    81481 gcggacaggt ccatggggac gaccgggtgg taggcatagc tctccacgac gaagttccag
    81541 ccccgctcgg ccaggttttc gaagaacttc aggctgtgcc agggggggag ctcggcgaag
    81601 agcagccggt atttctccgg aacggcgatc ccggccaccc cctgcgcgac gcgctcggcc
    81661 acctcccggt gcatgtcctc gtagagttcg aggaccttcg gcagatgccc cgtcaaaaag
    81721 aggcaccccg gcatggcgct ccagaaatcc ccgctgtgca tggggcaggg gcgatgcttg
    81781 cgcagctcgt tgacctcgtg gaagacccgc tggatcttca acgcgagatc gacgacggcc
    81841 tcgagcttcg cccagtccat cttccggccc aggagccgct ccagaaaggc cacgaaggcg
    81901 cgcaactccg ccacgacaaa gcggacggcc gcctcctccg ccccgtcctc ttgaaactcc
    81961 ttcaccccga gatggggcag ctcgagcacc cactgcggac agtcgaagta ccgccccagg
    82021 gcctggaacc acttgtaccg ggcgtcgcag gccgttcccg ccccgacgag cagggtcggc
    82081 cgggccatac ccccgagggg ggcgtccggc ggcacccggc ccgcctcgtc caccatcatc
    82141 cgcttcgtgt agccgagggt ggtccgtgca tacccgcaca ggtgggcggg aaacccctcc
    82201 gcatccgcga gggccagata cccctccgcc tgccccatcg aggcggcaat ggtggcgtaa
    82261 ttctccgggt agaccacctc cacgtccatg gccttgagga gtatgtcccc ctcccaccag
    82321 ttgagcatcg accaggccgt gggattcccg gcctggctgg ccatcagggt ggtgagatac
    82381 gcctcgttga cgacgttgcg caggggatac atgctcttga ggcggttgat cgccttgccc
    82441 ggtttcgggg gcttaagctc cggttcaccc atggtcggtt ccctccttcc tgtgagcccg
    82501 cgggaaactc gtccggcggg acggggcggt ttcctttata gccgtttttg cctgcgatga
    82561 caaaacgaaa atcatttccg gtttgccgca tcgtcgtttt atggtataga ggaccccctc
    82621 cggaggaaag gttcccgcgg agggaaccgt tcgttccgga tgaaagggga gagggatggc
    82681 catcaggacg catgaatttt tcaacttcta ttcccacctg accggcggcg tcgcggcggt
    82741 ggtcgggacc gtctttttga gtcttgtggc cagctattcc gcgtcggcgc tgatcaccgc
    82801 cctgatctac ggcctgt
//
LOCUS       DAOOLJ010000005        76152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_180_length_76152_cov_17.113802, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000005 DAOOLJ010000000
VERSION     DAOOLJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 76152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76152
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_76152_cov_17.113802"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1316)
                     /locus_tag="PLW16_01700"
     CDS             complement(66..1316)
                     /locus_tag="PLW16_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPB69649.1"
                     /translation="MISVQQALEQILSAISPLGSERVSLLAALGRVAAEDVDARRAIP
                     PRDNSAMDGYALRWEDTLGATRESPVILEVVEDIPAGKVPEKTVVAGTAARIMTGAPI
                     PDGADAVMKMEETEKDGSRVRIFAAASAGLDIRRAGEDVRPGERIVRKGDVLTPARIG
                     MLASVGRAVIDVHQRPTVAVLATGNELVDVDEAFLTEGKIVSSNSYSLAAQVLDCGAV
                     PLQIGIARDTREDLIAKFNAALRADIILSSGGVSVGDYDLVKEIMKEVGNRMQFWQVA
                     MRPGKPLAFGTLGGVPLFGLPGNPVSSMVSFEQFVRPALLKMMGRRNLFRRIIQACLD
                     EDVQKHAGARNFLRARVEFRDGRYHASTTGEQGSGILKSMVLANGLIVIPEAVTHARA
                     GEEVTVQLIDGSLEGMAEPEYLKD"
     gene            1501..2415
                     /gene="nadA"
                     /locus_tag="PLW16_01705"
     CDS             1501..2415
                     /gene="nadA"
                     /locus_tag="PLW16_01705"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPB69650.1"
                     /translation="MTDIQAWQRKVRRLLRERRAILLAHYYQRDEIQEIADLRGDSLA
                     LSIEAARTDAPVIVFAGVHFMAESAAIISPERTVLLPRPDAGCALADWITAAQLQKVR
                     EADPDLLVVTYINSSAAVKALSDICCTSANAVRVVNSLPEGRKILMAPDGNLARYAAR
                     HTTRDIIPWNGHCPVHHDITVDEVRRAKVAWPGAPFAAHPECNPAVLDEADFVGSTAG
                     ILRFARETDAQTVLIGTERGIFHTLREESPGKRFVPVSERLLCETMKYTTLPDIVASL
                     ETMSPVISVPEAVRVPAKRALDHMLALG"
     gene            2429..6109
                     /gene="mfd"
                     /locus_tag="PLW16_01710"
     CDS             2429..6109
                     /gene="mfd"
                     /locus_tag="PLW16_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPB69651.1"
                     /translation="MSRLVRTIIADRHALRDAALAGLVDRVARGASGLRVQGVSGSAR
                     ALLAAVLFRRLGKPLAVVTPTEGEARGLHRDMAFFLGEDDALLYPEGETVATDILTAQ
                     SESERDRIHVLDRLLDGAPVSVVASLPSMLRRVLPRDVLRASRIALSMGDAADRDDLA
                     RRLLAGGYERVTLVAEAGEFSLRGHILDVFPPGAHHPVRLEFVGDEIESLRAFDAASQ
                     RSIGEILSCTLIPAREAILTEERIGRAIRNVRRRAQTLELPRPVWESLSDAIAEGRTA
                     QLPARLLPLFYENLPGTEDPTAAEGLGTLFDYLPAEALFVLDNPAALRQSQEEFANEL
                     DGLILKAEREERFFLEREQTHLSGPDGWAAIQARPHLLVGGLTLTAGDAKPAEGGTTA
                     ETAVTLSTDRETTRLEGGRVSVAEEGLLKPLAERIRQWIASGYRVLFVCAGPESRDRI
                     GHLLAQYDLPATREEAFSLEDTVSQRGRGRLGLVEGRLTGGFHWPGLKLAVVSEEEIF
                     GRKHSRRRPRPVREGYFLKSFGELNEGDPVVHTDHGIGVYRGLTRLAVGNIENDFLLI
                     EYQEGDRLYLPVDRLDRIQRYIGSDGNDPRLDRLGGQSWETLKSRVKKSIREVAEELV
                     SIYAAREVMERENFSPPDRSYEEFASAFPFTETPDQVRAIEDVHLDMNAPKPMDRLIC
                     GDAGFGKTEVALRASFRAVMDGRQVAVLVPTTILAEQHYQTFLERFRDYPVQVACLNR
                     FRSRVQQAEIVAGIHRGTVDIVIGTHRLLQKDVAFKNLGLVIIDEEQRFGVLHKEKLK
                     KLRTLVDVLTLTATPIPRTLHLSLVGIRDLSIINSPPEDRQPIKTYLAEFDEDMIQKA
                     IRQELLRGGQVFFLHDRVRSLNTMARLVEKLVPEARVEVVHGQMKSTDIERIMAAFVR
                     RDFDVLVCTTIIGAGLDIPTANTMIINRADRFGLAQLYQIRGRVGRARDEAYAYLFVP
                     RGALLSPEARRRLEVILEFTEPGSGFRIAAGDLDIRGAGNLLGMSQSGHIAAVGYELY
                     TELMEKTIREIKGEGPPEERVRPEIHLGVPAYIPSDYMSDEHQRLVTYKRISLAADED
                     TLAEIRAELLDCYGFIPGEVDNLLEVIRIRNALTDLKGRKMGYDEKQLFIHFSPDSPV
                     DPARISDLAKLMNLPPPREKPVRTLARSLRKPAAARETPPGIRLTPDYRLYVPLPGLK
                     GDGVLAGARRLIDALGTGKPDA"
     gene            6180..7148
                     /locus_tag="PLW16_01715"
     CDS             6180..7148
                     /locus_tag="PLW16_01715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB69652.1"
                     /translation="MKAHQIVLLFLVCLFASSAAAATSVVERIVAVVNDEIITSYELN
                     SAFEPMEKRIEDTYKGPNKERLLQETRMDLLKRMIDKLLLEQEAKKTGIVVKDEEVME
                     TIRDMARRQNVSLDALKEALAKEGTTLEAYRNEIREQMVRMRILRRDVRSKIAISEEE
                     IGEYYLKHREVYEGKEAVHIKQILLPYSPNLDEEGKKKLREQAETIRQRIMAGEPFDL
                     MAVTYSQGPAAAQGGDLGFIEKGLMLPEVDRVAFRLKQDEVSEIIESSLGLHLLKLVD
                     KRGAGVKPIEAVRQEIVDRLEEQKLEVKYGDWIEGLRKRSHIEIRL"
     gene            7197..10022
                     /gene="uvrA"
                     /locus_tag="PLW16_01720"
     CDS             7197..10022
                     /gene="uvrA"
                     /locus_tag="PLW16_01720"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPB69653.1"
                     /translation="MNWIRIKGASQHNLKNVSLDIPRDRLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSTYARQFIGQMDKPDVESIEGLSPAIAIEQRAASHNPRSTVGTVT
                     EIYDYLRLLFARIGVPHCYQCGREILAQTIDMMIDHVLGLPEKTRVNLLAPIVRGKKG
                     EFQKDLKKLLKDGFVRVRVDGELRELADEIVLDKNKRHDIDCLVDRLVVKEGIRKRLR
                     DSLETAAGLTDGLVRVEVVGGEEILFSEKYACPDCGISLPELAPRMFSFNTPYGACPD
                     CGGLGSKMYFDEVLVIPDPSLSIREGAIAPWAGRNSVFFFQRLDALAQHYQFDINTPF
                     NRLPANIREALLYGSGKEKIKFYWDRRGRRFFYTKAFEGIINTLDRRYRETTSSAVRL
                     ELMKYISLRECPACRGARLKQESLSVTVSGRNIFDVCRLSIRECQAWFDTLPLTPQET
                     LIAERILKEIRERLRFLIDVGVDYLSLERSADSLSGGEGQRIRLATQIGSGLVGVLYV
                     LDEPTVGLHQRDNVRLIHTLKRLRDLGNTVLVVEHDADMMLSSDHIIDMGPGAGQEGG
                     EVVVQGSPAEVCRYGPSLTGQYLSGRVSIPLPSSRRPRPDRFIVLEGAYEHNLKDIDI
                     RIPVGVFTAVTGVSGSGKSTLVMRTLHKILERKLSRQRGRVGKIRRIVDLGGIERVIM
                     INQQPIGRTPRSNPVTYTGIFSPIRELFTGLPDARMRGYKPGRFSFNVKGGRCEACEG
                     NGLIKIEMHFLPDVYVTCDVCHGRRFNPDTLDIRYKGRNIAEVLDLTVLQALSFFENI
                     PSIRTRLQLLYDVGLGYLRLGQSATTLSGGEAQRIKLSRELGKRANSNTLYILDEPTI
                     GLHFADIQKLLDVLMRLVDMGNTVVVIEHNLDVVKSADHIIDLGPEGGPGGGWIVAEG
                     TPEEVAACEESLTGRFLKPILSRTGPA"
     gene            complement(10049..11176)
                     /locus_tag="PLW16_01725"
     CDS             complement(10049..11176)
                     /locus_tag="PLW16_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69654.1"
                     /translation="MMERRKQVAKITGIVNRGEAVGVIEALAEHGIVDAHLAVGRSDV
                     LHDKKWLFGASERTVIDEDPIEILTFLVDPALEDAALKLVLHAGRLGIAGRGMAFSET
                     VELLWAHELCAENRTADFTVASDIPLLSDLALVCCIAQRGEGDRMAMISLDTGTCVPA
                     ITFGQGTGVRDKMGLLRITIPAEKEVVHIVTDRHDAEGLMNLMIDVGDLDQPGKGFLY
                     CAPLNRGIVNTKIKRGVTRHAASIEQIVSVIDELKGGTKWRRRSGTAESEDAGKAYLR
                     DLTDLTMLCNEGRGPELVKAAMAAGAAGATIGRQRHVRPRQSPLSRLSPARECCNMIV
                     SPEQTPAIGEALRQAGAFDDETHGQVYARPVPKAFTYIGKS"
     gene            complement(11173..13293)
                     /locus_tag="PLW16_01730"
     CDS             complement(11173..13293)
                     /locus_tag="PLW16_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPB69655.1"
                     /translation="MTVRQQAGGRIRVGFRQAVGMLAPYVKSRLMAQIKAVALIIVYL
                     VLFQTLILGIPVARATVIAAGLALVIVGLTFFMEGLVLGLMPLGEVIGLKLPQKSVLP
                     VILVFSFILGVGATFAEPAIGVLKAAGASVKAWDAPLLFLLLNRYPDFLIYAVGIGVG
                     IAVVAGMLRFLYNWSLKPFIYLLIPVLVVFSVWAYFDKNMMFLTGLAWDCGGVTTGPV
                     TVPLVLALGIGVCRVVGSAGSGSAGFGVVTLASLFPILTVMSLGTLFVGSVPQPMSEA
                     DFFKLENRQATQALFSHPDEMAGYAFLNAAPENQLAFFGGDRDGMLAFLREASAEAKV
                     RERVFGPEADALKKWAASQGTREQQVAVFGDIEASTRYAASASALPERLDVFDLLARN
                     TRAAAQAIIPLSLFFILVLTLVLREKLPRADEILLGLVFAVIGMGLFNIGIELGLAKL
                     GGQVGGKIPSAFKAVELTDQTKTIHDFDPGVVQTALTQKGEESRFFYLKEKDRLLPLP
                     FNERQYNPETRQYTYVPTRGPLFGRSDGLGGILVVLLFAFILGYAATLAEPALNALGA
                     TVEDLTVGAFKKSLLMQTVAIGVGAGLAIGVAKIVWDLPLIWLLAPPYLLLLFVTAFS
                     TEEFVNIGWDSAGVTTGPITVPLVLAMGLGISTQVGVVEGFGILAMASVCPILSVLVV
                     GLWVTMKRRAAQASVPSGKGGTTP"
     gene            complement(13504..14427)
                     /locus_tag="PLW16_01735"
     CDS             complement(13504..14427)
                     /locus_tag="PLW16_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB69656.1"
                     /translation="MLKGLIYSLVSAFCLGMLAILIKLAYQTGLKDMALLQYRFLTAT
                     LVFGLYLLIRHPRLLRIRPATLAKTAFLGVVLHGWQSTCFFKALNYIPASTNALILYL
                     YPVIVTLLSLAVFRMKADRTIGLSLALLMGGCALVFYDAFLKALNVTGLLFAMGTTLT
                     FSVYLILSQIFLKGEEFLTVTFYVILFTAVPFLFVNNPLDIVHLAPRQLLLALAIGLV
                     PTALGVTFQFLAIRELGSARMSIFSTFEPVATVVMAWLVLGENIVFVQIAGMGLIIAG
                     IVLPNLRGCGLPRERPRHRPPAALDKAVGIR"
     gene            14655..14846
                     /locus_tag="PLW16_01740"
     CDS             14655..14846
                     /locus_tag="PLW16_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69657.1"
                     /translation="MGEITEKDRAMARRCVECPVCRRARRRQAGVAFWLVKNVEEGLC
                     PFCRAYEKVYGRKAHEPAP"
     gene            14869..15183
                     /locus_tag="PLW16_01745"
     CDS             14869..15183
                     /locus_tag="PLW16_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPB69658.1"
                     /translation="MGPTEGVETMLKHVVLMKFKTGVGETDVADLERGLAGLPAVIPE
                     IRGYDFGRDRRPERTFDFALVSTFDDETALERYKPHPRHQEVLARVKALCDELRVIDF
                     TY"
     gene            complement(15245..15889)
                     /gene="nadD"
                     /locus_tag="PLW16_01750"
     CDS             complement(15245..15889)
                     /gene="nadD"
                     /locus_tag="PLW16_01750"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPB69659.1"
                     /translation="MKWGLLGGTYDPIHFGHLRCAEEIREMFDLNRIIFVPASRPPHK
                     LEADITSFYHREQMVELAIEDNPSFSFSDIEHERGGVSFSVETVESFLGKYLKDLELY
                     FIVGQDAFHAIRTWKDWENLLVLCHFVVMTRPGYENRGLDGILPADFTARFVYDEACR
                     GYRGPTGQFIYFREVTFLDIAASRIRARVRAGQSIHYLVPDAVRHYIRKNVLYV"
     gene            complement(15886..17142)
                     /locus_tag="PLW16_01755"
     CDS             complement(15886..17142)
                     /locus_tag="PLW16_01755"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPB69660.1"
                     /translation="MNLHDQIVRIAREARQAADSLAHVPTDRKNAALLAMADALLARQ
                     AHLIAENARDLEAAREKGLPAAMIDRLTLKEQTLAGIAQGLREVAALTDPVGKVTGMW
                     RRPNGLLVGRMRIPLGVIGIIYESRPNVTADAAALCLKSGNAVILRGGSEAIHSNLAI
                     AACLTGVLRDHGLPTAAIQAIPLTDREAVHEMLQLDEWIDLIIPRGGEDLIRAVVSQS
                     RIPVIKHYKGVCHIFVDADADLDRAVPICLNAKTQRPGVCNAMETLLIHAAVAPAFLP
                     VVAERLREAGVALKGCPQARALLPDLAPATEDDWSREYLDLILSIRVVADLDEAIAHI
                     RRYGSLHTESILTENYTRAQRFLNEVNSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAFGPMGLEELTTTKFIIYGNGQVRT"
     gene            17363..18430
                     /locus_tag="PLW16_01760"
     CDS             17363..18430
                     /locus_tag="PLW16_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69661.1"
                     /translation="MDFLRIKAGPRAYEQLMDGGGVWDRISTYFGPAAGPRWLVAQGF
                     DLTLMKEGLLGRALPVWLVGSSAGAWRFAAWLQPEPEKSYAALREAYTGVTYARGDTP
                     DTILDKLLNVINAYIEDDALPFALNHKRYRLAILTARTRHLAASEIGWIQKGGLALCL
                     LGNMISRTNLARFAERVVFFHGPRPPAFCTHPEFQGRFIGLSPNNFKQAVMASGAIPL
                     VVAGVRDIFGAPRGVYRDGGLIDYHLSHDYSSRPDDLVLFFHHQERIVPGWLDKKLPR
                     RQPTPEALERVLMVFPSEAFLARLPYGKVPDRDDFLTFINEPAERIRYWNRSAELAAP
                     LGEMFLELVESGRIRRLLERF"
     gene            18512..19423
                     /locus_tag="PLW16_01765"
     CDS             18512..19423
                     /locus_tag="PLW16_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPB69662.1"
                     /translation="MKLKGVITVVRVPFLILALILGILGAAVAWYEAKRFGSPFHPGY
                     ALLATFGLVVAHAAVNIFNDYFDCRSGLDFKTRRTPFSGGSGAIPQGLLTIREALWLG
                     IGCLVIIIPIGLFFTWAAGWKMVPLLVLALCLIVLYTPLILKMGYPEWSPGLGLGILP
                     VMGAYFVHTGDYTASAFIASMPSFFLVHNLLLLNEFPDVEADWTVRRRTLPIVLGKRN
                     AAVFFSAVLLLTYLWILWAVMRQFMPVYALLALLTLPLAIQVIRGTFRYDEREVFMTV
                     MTKNVQVVLATQALLAAGYILGGIFPW"
     gene            19509..20447
                     /locus_tag="PLW16_01770"
     CDS             19509..20447
                     /locus_tag="PLW16_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69663.1"
                     /translation="MADPLFTQAARLQAMLESCELCPHRCRVDRTSGERGVCGLGASA
                     VVASTLVHHGEEPPLSGSRGAGTIFLASCNLGCPYCQNYQISRSTAGTVTDDEGLAEI
                     ALALEKRGCHNVEAVTPTPQAPRIAGALLAARRRGFALPFVYNCGGYENPEVIRLLDR
                     FVEIYLPDFKYGLAETALGLGNVPDYVPHALASLREMVHQVGDALELRERVATRGIIV
                     RHLVLPGHVANSLAVLKLIRENVSTAVPLSLMSQYTPVPAVKDHPDLGRRITRDEYDR
                     VVDAALDWGFSNLFIQDVDDRELTPDFDRESPFRWS"
     gene            complement(20467..24723)
                     /gene="glgP"
                     /locus_tag="PLW16_01775"
     CDS             complement(20467..24723)
                     /gene="glgP"
                     /locus_tag="PLW16_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPB69664.1"
                     /translation="MLKNREGYLFEVSWEVCNKVGGIYTVIMSKVNEAIRTFGQNYIL
                     FGPDLKTNLEFEETDEECWNRIREATAIKDIPCRFGRWQIPGRPKVILVNFSKRYNKD
                     QLLFRIWEDYGVDSIAGGWDYVEPVMFSYACGEVIETIYNLKLRTAGLPAVAQFHEWM
                     CGAGLLCLKKQVPEIGTVFTTHATMLGRTLAGSGMDIYTDMDSISPQREATNRNIAAK
                     YSMEVASAREADCFTTVSDITALEAKNFLGRAPDLVTPNGLDMGNIPDLTKDRGPALK
                     ARQRLLAAASRFLGREMPARTKIMVISGRYEYRNKGIDIFLGALGRLEKAMGEGTSVL
                     ACLFVIGGHTDLIPALQGDAVRIDPANPPLATYRLHNEASDPILEACNRVGLRNTAQN
                     RVNVIFVPAYLNGHDGLINLTYYEALSGCDLGVFPSYYEPWGYTPLESAAYSVPTVTT
                     DQAGFGLWAQSRMGENKGVLLVKRRGQSQDAIEGELYEVFRQCLSWSDEEERERRRQA
                     YDVAGQANWSDFYAHYRAAYGKALNVARERAVRLTSTEYRLETKHTFAGAASTQPHFR
                     QFTAVANLPRKIERLRELAYNLWWTWHPQARLLFSSLDPRIWGTMGNNPVRMLESVST
                     ERLIEAAENASYMNLYTQILKQFDGYLDDRLPDKNLAACPEIRWASPVAYFSTEYGLH
                     ECLPIYSGGLGTLSGDHLKTASDLNIPLVGVGLLYKKGFFHQSIDKDGTQIVDYPEND
                     FSNMPVQIVQDDRGLEVRIALDLPGRVLYANIWEVKVGRVSLYLLSTDTPRNTPQDRR
                     ITDCLYCSDPRTRIEQEILLGVGGVKLLKKLGIAPRVYHINEGHSAFLIFERISALMT
                     EEGLTFDEAAEVVRGSTVFTTHTPVEAGNERFDHDLTEHYFGSFVKWSGISWSRFMEL
                     GRKESGEDKPFYMTVLALKSAHLSNAVSRIHGEVSRRMWRDVWKGFHSSDLPIRSVTN
                     GAHLMSYIAPRMKELLDVYLGLDWERDLTDPERWARVQDIPDALLWRTRYELKQQLIN
                     FLRDDVARHGAKYGYAKTWREELYARINPSALLIGFSRRFAPYKRANLILSDLDRLDR
                     ILNHPTRPVAIVFAGKAHPNDSRGRDLIQQVVEIMRDERFRGKIFLLENYDIRIARRL
                     VQGVDVWLNTPRRPYEASGTSGQKVVVNGLLNLSTSDGWWPEGFDGTNGWTIGPVVKE
                     RSEDTPNADLDDAQALYELLEDTVAPLFYDRDVSGVPEKWVGMIKRSMRTLAPSFNTE
                     RMLLEYFHDLYLPSAERSCELVDDQYRLARELADWKRKLPMRFSSLRLLEVTVEGIHG
                     DAVMVDQPLNVTASVDPGKLDPEEILVELMIGRKDGADFAGRPDCMPLKMVGCAENGV
                     LTYALDYRVRQNGPHRYGVRVLPFHPRLSSLHETGLILWG"
     gene            complement(24717..25943)
                     /locus_tag="PLW16_01780"
     CDS             complement(24717..25943)
                     /locus_tag="PLW16_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPB69665.1"
                     /translation="MPSVCLYFQIHQPRRLKRFSVFDIDAAGAPAFARYEAEEENRRI
                     LTRVVEKCYRPATRLLGNLVATHAGRFRCAFSITGVTLDRLAIDHPDVLDAFRALAAS
                     GCVEFLNETYEHTLAFPRDPEEFRTQVRRHRERIRDAFGREAVTFRGTELIFSNALVE
                     RVAELGYRVMLAEGTEPLLRGRSPNRVYGTAVDPRVRVLLRNYRLSDDIAFRFSDRGW
                     TGFPLTAAKFAHWIHALADDARVVNLFMDYETFGEHQWAETGIFHFLEALPGEILKHP
                     AFRFQTPAEAAEAHPIEGILDCPGIVTWADRERDLTAWQGNAMQQDALRALYRLSVQV
                     QRRKDPDLLNTWRLLQTSDHFYYMCTKWSADGDVHRYFNPYASPYDAYVNFMNVLDDF
                     TERVTAARPELEEPRC"
     gene            complement(25957..27237)
                     /locus_tag="PLW16_01785"
     CDS             complement(25957..27237)
                     /locus_tag="PLW16_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB69666.1"
                     /translation="MRILMFGWEFPPRISGGLGTACLGLTRAMSELGQTITYVIPCAR
                     TGEEPGHVRLVSAAEVPASDRPGWDAEGAAPPAGMPGRPLDVLLRIIDSPLHPYLRED
                     RYPGISGGAAGMLAPSGNYGSDLMAEVLRYGKAAGRIAAEASFDVIHAHDWMTVPAAL
                     EARRVSGRPFVYHVHATEIDRSGNNVHPAIFAVERLGMSLADRVIAVSHYTRGLIVRH
                     YGIPAGRVSVVHNAVSRREAAPAYRAFKPPGKKWVLFMGRITFQKGPDHFVAAAAKVL
                     SVLPDVRFVMVGAGDLLPQTIERVAELGLGRQIHFTGFLDGEEVERMFALSDLYIMPS
                     VSEPFGITPLEAMLHDVPVIISKQSGVGEVLRHVPKVDFWDVDALAWTIVAILTQPEL
                     AEAIVARSRGELEALRWETAALRVLDVYRGLLGP"
     gene            27475..28131
                     /locus_tag="PLW16_01790"
     CDS             27475..28131
                     /locus_tag="PLW16_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB69667.1"
                     /translation="MKLFAISDIHGATKPIEAAAALIRAADGVVVSGDITRGKTRSEA
                     EEVVACIEQYSTRILAVHGNWDRPEVMTFLEDKGYSLHGTGRTLDGTGFFGLGGSSPT
                     PIHTATEYTEKDIDGLLRVGYQTVHEAARTVLVSHVPPRGVRDRSFLGLRGGSHSLKR
                     FLETHPVSLCLCGHIHEAAGIERFRDTLVANAGSFKRGHYLTVELGPTITVTAGRVAH
                     "
     gene            complement(28199..29380)
                     /locus_tag="PLW16_01795"
     CDS             complement(28199..29380)
                     /locus_tag="PLW16_01795"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPB69668.1"
                     /translation="MDTVYLDHAATTPTDPEVMAAMMRYFGGRFGNPSSIHACGRDAR
                     EALETARSQVAELIGAAPEEIVFTGGGTEADNLAVLGAAAARPPARNHLVTTAIEHPA
                     VLNACKSLVEAGCKLTVLAVDPLGRIDPEAVREAIRDETFLVSIMHGNNEIGAIEPIA
                     EAARVARERGVPFHTDAVQTAGKIPVAVEALGVDLLSLASHKIYGPKGVGALYVRRGT
                     RLAPRQFGGHQEGGLRSGTENVPGIVGMGRACEIARRDLAEQGVLLASLRDRLEGAIR
                     RALPELRFNGHPELRLPNILSVSVDGVTGEVLVREMDKQGFAVSAGSACTSESPTLSH
                     VLTALNLSVGEALGTVRFSLGRTNTAEQIDRAADAFIRIASRLQALAELEDAAGRRGC
                     R"
     gene            complement(29545..30006)
                     /locus_tag="PLW16_01800"
     CDS             complement(29545..30006)
                     /locus_tag="PLW16_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69669.1"
                     /translation="MAKEYKTLKTEIRLPYELSYGPVWTRFFEGMREKKIYGTKCEKC
                     GRILVPARSFCPRCFVDTNAWVEVAQTGKLTAWSYINYRYFEMPTEPPFIGALIRLDG
                     TDVDFLHLLGGIDLADLDKVRGIVHNGMQLKAVWQEERIGHILDLEHFAPV"
     gene            complement(30037..31203)
                     /locus_tag="PLW16_01805"
     CDS             complement(30037..31203)
                     /locus_tag="PLW16_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69670.1"
                     /translation="MGRAVGIVGVGQTHHGKRMEISYPDLVREAVVRVFEDTGLSPRD
                     IDGVVSGTMPSMMEGIALTHFYFADAMQAVGKPILKTETCGSTGVSIAHTAYYWISSG
                     MADCVLAVGHEKMNEGDAQATMTTVVEPFYQRFFIAGAPGVFSMQSQMWAERYGIPQE
                     KIREAAAHISVEHHEAAFANPYAHMKIHVTMDDVKNARLITYPVRLLDVCPTSDGACA
                     VIFMSEEKAKQLGRPVAWVKGVGYRGEEYFFGDSDKGVWQSAVGAAKQAYDQAGIKNP
                     RKELDVAEVYNPFTFQEMLFYECFGFCDLGQSPDLVLKGVFDRTGELPCDPSGGTLCT
                     NPIGATGLVRVAEAALQVMGRAGDHQIAGAKLALSHAMGGVDQFNGVMIVGSEL"
     gene            complement(31216..32358)
                     /locus_tag="PLW16_01810"
     CDS             complement(31216..32358)
                     /locus_tag="PLW16_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69671.1"
                     /translation="MGDRVAIVSVAQSPGAESKDNFYDQAYRAARECLDKAGLGREDV
                     DTVVSASSDIFHGGISCANAYYWDTTGAFLKNASRQDGESLFAFMYAVMRIMTGQYET
                     ALVISLCKGSENPPNDACTLMFADPFYQRDVGLTETVAAALQMNLYMERYGVTREQCA
                     AVAAKNLGNALKNPYAHRKGKYTVSDILRSPTLADPLTELQAAPKSEGMLAMLLAGEA
                     KARKLTDKPVWFKGYGSSLDTYTLGDRDLLRGELTRAAKRAYDMAGIKNPKEGIDVAE
                     ICEPYAFQELLWSEDLGFCGAGEGGKFLESGATQIGGSLPINPSGGVLANNPYVSRGL
                     QRLIEATLQLRGEAGEHQVPKSVKTALAHGTHGFAGQCHAVAIIGR"
     gene            complement(32605..33495)
                     /locus_tag="PLW16_01815"
     CDS             complement(32605..33495)
                     /locus_tag="PLW16_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPB69672.1"
                     /translation="MAPSDVIEINAAKRTLRLKDALSGSIFTGTDKVRSGYGNGDCLY
                     FDFRHRVFAVADGTERFPWASRDILCRLSDALMQAGVPASAADWKELINRRVYAGQKY
                     QHKTTFSCVAVSGDDHEVVLTVAHGGDSGVLVIDSISGDVLFETGRNMVFAGRSREIV
                     DVIECRVTDRNARVALYSDGFDDLVRFCINRSFLSRLSDAFAAFPIDGLGEQMHRLLG
                     KHAGRFEHDDIALMVMDPFRLLGGLEEKRVLIGGTQPHEEVHFRTGCLNGELDRWVPY
                     AGWSSASEAFLKLGITIQEA"
     gene            complement(33499..33963)
                     /locus_tag="PLW16_01820"
     CDS             complement(33499..33963)
                     /locus_tag="PLW16_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69673.1"
                     /translation="MAEKEQLIIESGDAAQPFKYAVGMHGSKFFTETRDNMRLMGLKC
                     PKCGKVYVPPRGVCGKCFVEMDDWVEVGPKGVIGTFTILRFAFIDPETGQQKPVPYGY
                     GFIKLDGADTLLQHYIDIKDESKVKIGARVEPVYAQERKGNTRDIEYFRIID"
     gene            complement(33975..35132)
                     /locus_tag="PLW16_01825"
     CDS             complement(33975..35132)
                     /locus_tag="PLW16_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69674.1"
                     /translation="MAKRVAIVGTGQTFHKSHRPDVNGQELINEAVQRALADADLTMK
                     DIDAILIGNMDHFEGINYVDCWSVDGCGGVMKPVIKITTGGTTGASLAIGGYHMVGSG
                     MFEKVLIIGWEKNSESDTTGAITTAFDPIWDRQVFAGAISGLAAEAQAYMARFGASDR
                     DGARVSVRDRKHAMNNPHAQLRKEVSLEDVLASPMLADPIHLLDVCPRSDGACAVIMA
                     SEDSAEKICPVPDWIWGTSAHNTYSYLGDGDWGRLTSMEQCAKEVFKKAGIKEPRKEL
                     DVIEMYQPYSFAGLIWIEDMGIVGHGEAPQFVWDGNTDMGGELPVNPSGGVIACNPIG
                     ATGLIRCAEAAMQVMGKAEGRQVPDVKFAVSTGFGGCMWTNMLLHGKRKPN"
     gene            complement(35144..36289)
                     /locus_tag="PLW16_01830"
     CDS             complement(35144..36289)
                     /locus_tag="PLW16_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69675.1"
                     /translation="MDRTAIIGVGMTTIEAEKRRETFADMTWEAVNKALSDAGMTIAD
                     IDNVVTTSSDFWDGRTISLMAVGDVSGAAEKNVSCVEGDGTYGAFYANARALSGSYKT
                     TLVTGHSKGSQCVSSLITNAAFDPIYERVLGMDMITASAMQANAYMNRTGVKPEDAAL
                     VSVKNHGNALKNPMAQLPMNITVDDVMASEMIADPLRKLDCSPVSDGCCAVIIAHESV
                     ATKFKQKPIWIRGLAFCTDSFFFGDRDLARSKALTEAAKKAYAMAGIKDPRKEIDLVE
                     LYDAFSYQELLWLEEMGLADYGKAAARLKAGEFNLDGALPVNPSGGLLSGHPVIAAGL
                     YRIAEIVLQLRGDAGAHQVKKAKTGLAHGVNGLCGQSHCVWILDKEK"
     gene            complement(36299..36766)
                     /locus_tag="PLW16_01835"
     CDS             complement(36299..36766)
                     /locus_tag="PLW16_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69676.1"
                     /translation="MSEIFKGIEPMVHNSKISVPYSWWAGDTASKFFISLRDARKIIA
                     PRCGKCGKVFMPPRKVCPTCFTENTDWVDVSDEGTVVSYSVSRRQLEAIPGDKKVPVI
                     WGLIKLDGADTALLHYLDEVDPGSVTIGMRVKAVFAESRTGGIRDISHFKPVK"
     gene            36965..38773
                     /locus_tag="PLW16_01840"
     CDS             36965..38773
                     /locus_tag="PLW16_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB69677.1"
                     /translation="MSQVNLAQIFRHQAQKYGERLAVEKRLRNQWQGWTWKTYYDTAR
                     ALGLGLYRLGVRRGDRVGLLSENRLEWIGADMGIICIGAITIPIYVTLPAAEVAYILG
                     NSDAKVFIAENQAAADKALKMNADLPELQKIVVIEADGCDLSHPLLMSFSALLDLGRG
                     LAAEEPDLFETLGDAIRTEDMATFVYTSGTTGPPKGAMISHRNILAIFDALDTVVPAY
                     ETDETVPFLPLCHVFERIAGHFYGMKVGITAHYAQSFDTIVEDIKTKRPTIVLAVPRV
                     CEKIYARIQSEVKSQPAWKQGIFRWAQRVGSELSLLKEQKKPVPPLLDLRYRLAYKMV
                     YQKLNEGLGGRVRWMTAAGAPLSREIADFFVSSGVFVIEGYGMTETTAPVSLNVLGDY
                     RFGTTGRPLPCNRVKIAPDGEILVKGDNIISGYWKMPEQTAAAFTPDGWLMTGDIGHL
                     DGDGFLVITDRKKDLIITAGGKNIAPQNIEGLFMKDPLFENFVVIGDRRKYLTALVTL
                     NLEEAARLARSAGLAFVDAQDLPEREDFRSIVEKHVAERNQHLAKYETIKYFRIVPHA
                     FTEQTGELTPSLKVKRKVVLEKYQELIEGMYPADVV"
     gene            complement(38850..40334)
                     /gene="cysS"
                     /locus_tag="PLW16_01845"
     CDS             complement(38850..40334)
                     /gene="cysS"
                     /locus_tag="PLW16_01845"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB69678.1"
                     /translation="MMSVRFYNTLTRRKEDFSPLQVGKAGIYVCGITAYDLCHIGHAR
                     AAIVFDVLARFLRYRGYDVTYVKNFTDVDDKIIARAEREGRTIGEVAETFIAAHDEDM
                     AALGVERATVTPRATEHIDGMIALIRTLLEKGLAYVVSGDVYYAVERFAGYGKLSGRG
                     LEEMLAGARIDVNEKKTNPMDFALWKGSKPGEPGWDSPWGKGRPGWHIECSVMSQRYL
                     GDTFDIHGGGEDLIFPHHENEIAQSEGATGRPLARVWMHNGFVRVNKEKMSKSLGNFS
                     TIREMLAVHPPEVLRLFILQSHYRSPVDFSDQALRETRTGLDRFYETRRKIQAALSGG
                     PAADGTAGERLTGGHRRLYEKATGLQERFAGAMDDDLNTARAIGYIFDGVRQVNAYLD
                     AEGQAASPERTLVLAAARQAFDRCGDVLGLFRQDPRDYFAMDQRRETGRRGLEVAEIE
                     DLIAQRLTARQARNWAQADAIRQRLAEHGVILQDGPAATTWKFA"
     gene            complement(40388..41428)
                     /gene="argC"
                     /locus_tag="PLW16_01850"
     CDS             complement(40388..41428)
                     /gene="argC"
                     /locus_tag="PLW16_01850"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPB69679.1"
                     /translation="MMKVGIYGASGYTGQELLRLLLRHPRVTVAAVTSRRYRGKAVAE
                     LYPVFADLTDLVFMDATPAAFAELAEVVFLALPHGVSMSVAPGFLAAGCKVIDLSADF
                     RLREASVYEQWYARHLAADTLPAAVYGLPELYRDAIRRTVLTANPGCYPTSIILGLAP
                     LLKAGWLKTDTIIADAKSGVSGAGREPVVTSLFCEVDGGFKAYKVGGQHRHTPEIEQE
                     LTALAGEAIRISFTPHLLPLNRGILSTLYGTLNRDASTADVKALYEAFYERERFVRIC
                     RSGNFPNLAAVRGSNYCDIGVTVDERTGRVIVVSVIDNLVKGAAGQAVQSMNLMCGFE
                     EAAGLEMLPLFP"
     gene            complement(41471..41863)
                     /gene="rpsI"
                     /locus_tag="PLW16_01855"
     CDS             complement(41471..41863)
                     /gene="rpsI"
                     /locus_tag="PLW16_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB69680.1"
                     /translation="MTDKRFYATGRRKSSVARVYMKEGSGVLTVNKRNFDDYFTRDSL
                     KMLIMQPLEMTGQKGKLDFFVNVCGGGVAGQAGAVKHGIAKALIEYDPEMRSLLKKAG
                     FLTRDSRMKERKKYGQPGARKRFQFSKR"
     gene            complement(41923..42351)
                     /gene="rplM"
                     /locus_tag="PLW16_01860"
     CDS             complement(41923..42351)
                     /gene="rplM"
                     /locus_tag="PLW16_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPB69681.1"
                     /translation="MKTYQAKKEERAEAWYLVDAEGQVLGRLASEVARRLRGKHKPVY
                     TPHTDTGDFIIVVNCEKILLTGRKLTDKVYSRHSGYPGGIKQVTAGKLLETAPEKVLH
                     TAVRGMLPKNSLGRQMLRKLKIYKGNDHPHQAQCPQTVVL"
     gene            complement(42451..44634)
                     /gene="lptD"
                     /locus_tag="PLW16_01865"
     CDS             complement(42451..44634)
                     /gene="lptD"
                     /locus_tag="PLW16_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HPB69682.1"
                     /translation="MYGITGKTIRFVLMALLSFVLNGMGGQGLAMELAPREGPVDIEA
                     DSLSYDRDTDTYHAQGNVVIIYSGGRLTAQSVLLDHRTDTAHAEGNVRLTSGRDVLEG
                     DRMTFHLTDKTGVVHAGKVFVAQNHFYLRGDKIAKTAEATYRVEGAEATTCDGPRPDW
                     RLTSREINVTVDGYGTMKHGKLLIRNIPVLYAPYFIFPAKTTRQSGFLLPYLAHSDDH
                     GWDAEVPFYWAISRSLDATFYSRYMDKRGFKEGTEFRYFAGERSSGVLYGDLLNDRKR
                     VTETSGPVSRDWRSDRLRWSLFLNHETVFTEGFYLRADIARVSDAWYFKDFDDRNYYR
                     DHRDRGGRERFEKVSFDGDASLRSLDSTVRLVKDWSRYNLTALARYTDDFTVPSNDVT
                     LQRYPEIILNRATLPLFGQPVYYGLKATYDYLYRGEGQRGHLYDVAPVFTLPIKLGRY
                     AQFTPEVGVRATLWDRDDDGVARGDRHGDREVFHAGFSLNTELSRVFDRPGKSLERLQ
                     HTIKPEIAYAYADATLERQVALPDYVARVNAQNLVSYGITNVLTAKVRGNDDQIAYRE
                     LLRLKLAQQFDIREARRDRDGPGDPGRRPFGVLALDLVMAPIPEISLVVRNLYDVHEH
                     AWTRTNYDLNARVGRVNLGLGYHNTRDILEEINLAVKADLTRTIRLDYTLRNNLRDNV
                     KVENYVQVTYHRQCWSIGLGYEKKEDDQRVTLLLSLYGLGQAGVH"
     gene            complement(44703..45968)
                     /locus_tag="PLW16_01870"
     CDS             complement(44703..45968)
                     /locus_tag="PLW16_01870"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPB69683.1"
                     /translation="MTSLPPPTFPPSFGPAQIRFGLGPIKRLLARLGDPQERYRTVLV
                     AGTNGKGSVSAMLAEILKQGGVRAGLYTSPHLIDFRERIRVGGRPISRAQARTLIREV
                     RAALTEEVTYFEFLTALAFTHFAREAVPWAVLEVGMGGRLDATNVVRPAVSIISNISL
                     EHRDYLGRSLRDIAREKAGIVKTGGVCLTAATQAAVRDTLAETCRRRRVRLLRLGGEI
                     RIRRHDDGSFSYFGVKTVLRHLHLALAGRHQQANAALAVGAAEILSERGVPLDEAAIR
                     RGLLNVRWEGRLETLRERPRVVVDGAHNPAGMAALVRSLRTEFSYARLILILGVLKDK
                     DYARMIRQAASLDATIIATRPRNERGLPAAHLAAAAARWHSRVEAVEEPREALRRALL
                     IAGPRDLVCVAGSLYLIGEIKACFGRVSL"
     gene            complement(45993..46769)
                     /locus_tag="PLW16_01875"
     CDS             complement(45993..46769)
                     /locus_tag="PLW16_01875"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HPB69684.1"
                     /translation="MDIRVLGCHGSQLPGLNTTSFLLNGHLLIDAGAVANVLNIEEQR
                     RIDQVLVTHAHLDHIRDIAFLADNLCYGERHKPLTVRSTPFVLQMIHAHVFNGVIWPD
                     FSILPSREKPVIRFKALVPGKTEWVGDLQIEAIKVDHTVETVGYVVRSAEGTVIFFGD
                     TGVTEPLWERANGVDDLRAIFIETSLPDGMKEMASQTGHLTPALLKEELKKLCHPHPE
                     VYLYHMKWDCHDVIREQIAVIGNHRIHFLKDGQRIRIDPC"
     gene            complement(46869..47129)
                     /locus_tag="PLW16_01880"
     CDS             complement(46869..47129)
                     /locus_tag="PLW16_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPB69685.1"
                     /translation="MAEVKIGEVVKFFAKPSVAAVKVTGGELKVGDTVKFTGHTTDLT
                     HTIDSMEVNNQKIDTAVTGDFIGVKIGDRVRPGDEVFRVIPD"
     gene            complement(47345..47743)
                     /gene="rplQ"
                     /locus_tag="PLW16_01885"
     CDS             complement(47345..47743)
                     /gene="rplQ"
                     /locus_tag="PLW16_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPB69686.1"
                     /translation="MRHGKFGKKLGRSSSHRQAMLRNMVTSLIRTEKITTTDAKAKVL
                     KSLADRMVTLGKRGDLHARRQALSFIRDRAMVIKLFDELSPRFRERPGGYTRIVKMGY
                     RHGDNAPVSVIEFIPAVTVDKPKKKTAAKK"
     gene            complement(47774..48793)
                     /locus_tag="PLW16_01890"
     CDS             complement(47774..48793)
                     /locus_tag="PLW16_01890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPB69687.1"
                     /translation="MQKNWRSLIRPKRIEIDEGTHTPFYGEFTCQPLERGYGITLGNA
                     LRRVLLSSIQGAAIVSVRFDGVLHEFSTIPGVKEDVTDIILNLKGIRLKLHGDGPRTV
                     RIDTKKEGAITAADIIADATVDVLNPEHHIATLFGKGAFHAELVVQNGKGYVPAQKEK
                     DLEQPEGTINIDATFSPIRKVNYTVTYARVGQVADYDKLVLEVWTDGSVLPEDAVAYA
                     SKILKEQLDVFINFEELEEQGEAVETGEVEGINEHLLRNVDDMELSVRSANCLKNAGI
                     NLIGELVQKTEAEMLKTKNFGRKSLNEIKEILAEMGLGFGMKLDFPPWNEMEKKGSEN
                     GSQAD"
     gene            complement(48842..49468)
                     /gene="rpsD"
                     /locus_tag="PLW16_01895"
     CDS             complement(48842..49468)
                     /gene="rpsD"
                     /locus_tag="PLW16_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016801712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPB69688.1"
                     /translation="MARYRESACRLCRREGLKLFLKGDRCYSEKCAFERRGYAPGEHG
                     QMRRKFSDYGVQLREKQKLRRMYGLLERQFRGYFDRADRQKGITGTNLLVGLERRLDN
                     MVFRMGFANSRAEARQLVRHNHFLVSGKPVNIPSYQVKVGDEVQVREGSRNVQRIAEA
                     METVARRGVPQWLELDRTNFRAVVKTMPGREDLTMPVQEQLVVELYSK"
     gene            complement(49559..49951)
                     /gene="rpsK"
                     /locus_tag="PLW16_01900"
     CDS             complement(49559..49951)
                     /gene="rpsK"
                     /locus_tag="PLW16_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPB69689.1"
                     /translation="MAKAVRKTGKKKEKKNIAEGVAHIQSTFNNTIVTITDLSGNVIA
                     WASSGMQGFKGSRKSTPFAAQMAAEDAVKKAKEHGLRSVQVYVKGPGSGRESALRSLQ
                     LAGLNITLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(49967..50335)
                     /gene="rpsM"
                     /locus_tag="PLW16_01905"
     CDS             complement(49967..50335)
                     /gene="rpsM"
                     /locus_tag="PLW16_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPB69690.1"
                     /translation="MARIAGVDLPKNKRMEIALTYIYGIGRSRAKQILAKAGVSESTK
                     TDELADMEINAIRNVIDREQKVEGDLRREVSMSIKRLMDIGTYRGLRHRKGLPVRGQR
                     THTNGRTRKGPRRSIAGKKK"
     gene            complement(50365..50478)
                     /gene="rpmJ"
                     /locus_tag="PLW16_01910"
     CDS             complement(50365..50478)
                     /gene="rpmJ"
                     /locus_tag="PLW16_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPB69691.1"
                     /translation="MKVRSSVKKICDKCKIIKRRGVLRVICENPKHKQRQG"
     gene            complement(50559..50777)
                     /gene="infA"
                     /locus_tag="PLW16_01915"
     CDS             complement(50559..50777)
                     /gene="infA"
                     /locus_tag="PLW16_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPB69692.1"
                     /translation="MAKEEPIEVEGRVIEPLPNAMFRVELENGHRVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRTK"
     gene            complement(50851..51603)
                     /gene="map"
                     /locus_tag="PLW16_01920"
     CDS             complement(50851..51603)
                     /gene="map"
                     /locus_tag="PLW16_01920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB69693.1"
                     /translation="MVILKSPEEIEKIRKSNRIVAEILAELKAHAKPGVTTGDLDRLS
                     ESLTHRRGARPAFKGYRGYPYSLCASVNQEVVHGMPSDGRVLKDGDLVSLDFGVDYQG
                     YFGDAAITVAIGQVSREARRLMDVTEAGLYEGIREARAGNRLGDISAAVQACVEAAGC
                     SVVRDYVGHGIGRELHEDPQIPNYGVKGRGVALKAGMVLAIEPMVNLGTYRVQVLEDG
                     WTVVTADGKLSAHFEHSVAITDRGPEILSLLR"
     gene            complement(51636..52943)
                     /gene="secY"
                     /locus_tag="PLW16_01925"
     CDS             complement(51636..52943)
                     /gene="secY"
                     /locus_tag="PLW16_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPB69694.1"
                     /translation="MLDGLKNIQKIPELQKRILFTFVLLTVYRIGAHIPTPGIDTVAL
                     GAFFEQAKGSLLGLFDMFAGGALSNLSVFALGIMPYISASIILQLLTVAVPHLERLSK
                     EGEAGRRKITQYTRYGTVLLSIIQGFGIAIGLENMAGPTGASIVITAGWSFRLMTVIT
                     LTAGTAFIMWLGETITEKGIGNGISLIIFAGIVCRGPEAILNTFRLLSSGEMGFFFII
                     LLILMMVAVVGVIVFVESGQRRIPVQYAKRVVGRKVYGGQSTHLPLKVNTAGVIPPIF
                     ASSIIMFPATIANFIPHPWMKTVADMMVPGRWGYELLYVAFIVFFCYFYTAVTFNPVD
                     VADNMKKYGGYVPGIRPGKKTAEYIDDVLTRLTFSGAIYVSAVCVLPSILISYFNVPF
                     YFGGTALLIVVGVALDTAGQIETHLLTRQYEGFMKKGRIARRR"
     gene            complement(52945..53388)
                     /gene="rplO"
                     /locus_tag="PLW16_01930"
     CDS             complement(52945..53388)
                     /gene="rplO"
                     /locus_tag="PLW16_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPB69695.1"
                     /translation="MNLSQLKPPEGSRKKRKRVGRGDGSGHGGTACKGAKGQNARSGG
                     KTKPAFEGGQMPMARRLPKRGFKNPFRRVLATLNLDQLKRFPAGSVIDKDMLVACGLI
                     RKKNDGVKILGKGELTGPLTLKVDQVSQGAKAKIEAAGGSVVEVA"
     gene            complement(53385..53585)
                     /gene="rpmD"
                     /locus_tag="PLW16_01935"
     CDS             complement(53385..53585)
                     /gene="rpmD"
                     /locus_tag="PLW16_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPB69696.1"
                     /translation="MAKKLKITLVKSMIGRPTKQRQVLRGLGLGRMNRSVVLEDTSAI
                     RGMVQKVIHLVAVEDLEGGAQG"
     gene            complement(53604..54083)
                     /gene="rpsE"
                     /locus_tag="PLW16_01940"
     CDS             complement(53604..54083)
                     /gene="rpsE"
                     /locus_tag="PLW16_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPB69697.1"
                     /translation="MEEQELLDRVIAINRTAKVVKGGRRFRFSAVVVVGDGQGSVGAG
                     LGKAHEVPEAIRKGMEQAKKNMVKVSIRERTIPYEVVGRYGAGRVLLKPASEGTGLIA
                     GGAVRAVLEVAGIHNILTKCLGSHNPHNLVKATLDGLVQLKDPATIFQLRGKSFGEA"
     gene            complement(54130..54495)
                     /gene="rplR"
                     /locus_tag="PLW16_01945"
     CDS             complement(54130..54495)
                     /gene="rplR"
                     /locus_tag="PLW16_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPB69698.1"
                     /translation="MATKKIEKIRAKREARVRSQIKGTRAVPRLSVFRSTRHIYVQAV
                     ADDVGQTLAAASTLSPELKERLERMKKSEAAREVGKLVSERLKAAGVEQVVFDRGRYL
                     YHGRVKALADAAREAGLKF"
     gene            complement(54554..55093)
                     /gene="rplF"
                     /locus_tag="PLW16_01950"
     CDS             complement(54554..55093)
                     /gene="rplF"
                     /locus_tag="PLW16_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPB69699.1"
                     /translation="MSRIGKKAIEIPERVQVRWDQERITVEGPKGALSRTLPAGIQVG
                     IDDRTITVTKLEDTRTGDAYQGLTRTLIANMVTGVSTGFVKNLEVVGVGYRAEMAGDA
                     LKLLVGFSGPITYQVPPGVKVSVEKQVNISVSGIDREQVGRVAAEIRGIRKPEPYKGK
                     GIKYAGEYVRRKVGKSVGA"
     gene            complement(55120..55521)
                     /gene="rpsH"
                     /locus_tag="PLW16_01955"
     CDS             complement(55120..55521)
                     /gene="rpsH"
                     /locus_tag="PLW16_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPB69700.1"
                     /translation="MGMTDPIADMLTRIRNANRAHFKSTDVALSRMNLSVAKVLKKSG
                     YISGYDVKKNAQGRQVLRIFLKYVDAKRTVMTDIQRVSKPGRRIYVGSGEIPKVLNGY
                     GISILSTSRGIVTDQEARELNVGGEILCKVW"
     gene            complement(55547..55732)
                     /locus_tag="PLW16_01960"
     CDS             complement(55547..55732)
                     /locus_tag="PLW16_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPB69701.1"
                     /translation="MAKKSLIAKAARKPKFSVRAYNRCAVCGRPRAYYRKFDMCRICL
                     RNLASKGEIPGVIKSSW"
     gene            complement(55779..56321)
                     /gene="rplE"
                     /locus_tag="PLW16_01965"
     CDS             complement(55779..56321)
                     /gene="rplE"
                     /locus_tag="PLW16_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPB69702.1"
                     /translation="MAARLKSYYEKEVVPALIEAFKYQNRMQVPKLEKIVINMGLGEA
                     IQNVKILESAAQEMAAICGQKPVITKARKSIATFKLRQGMSIGCRVTLRRERMYEFFD
                     RLANVALPRIRDFRGISLKSFDGRGNFSLGLREQIIFPEIDYDKIDKVKGMNIAIVTT
                     AKTDEEARLLLKLMGIPFRS"
     gene            complement(56334..56645)
                     /gene="rplX"
                     /locus_tag="PLW16_01970"
     CDS             complement(56334..56645)
                     /gene="rplX"
                     /locus_tag="PLW16_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPB69703.1"
                     /translation="MRLKKGDTVKVIAGKEKGKTGKILKIVGDRERVIVEKVNMLKRH
                     QRPDAKGKGGIVEKEGSIHLSNVMFVCNKCDGTVRVGYRLLDDGKKVRVCGKCQEILD
                     A"
     gene            complement(56670..57038)
                     /gene="rplN"
                     /locus_tag="PLW16_01975"
     CDS             complement(56670..57038)
                     /gene="rplN"
                     /locus_tag="PLW16_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPB69704.1"
                     /translation="MIQMQTVLNVADNSGAKKVACIKVLGGSKRRYASLGDVIVVSVK
                     EALPNSKVKKGDVMKAVIVRTVKEVRRPDGSYLKFDDNSAVLITNQLEPVGTRIFGPV
                     ARELRAKQFMKIISLAPEVL"
     gene            complement(57129..57386)
                     /gene="rpsQ"
                     /locus_tag="PLW16_01980"
     CDS             complement(57129..57386)
                     /gene="rpsQ"
                     /locus_tag="PLW16_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPB69705.1"
                     /translation="MSERGNRKTIQGVVLSNRMDKTIVVRAERIVKHPVFQKYIKRYE
                     KYKAHDETNQCAVGDRVLIVECRPLSREKRWRMTEVLERAK"
     gene            complement(57383..57580)
                     /gene="rpmC"
                     /locus_tag="PLW16_01985"
     CDS             complement(57383..57580)
                     /gene="rpmC"
                     /locus_tag="PLW16_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPB69706.1"
                     /translation="MKIKEIRDLSWAELQQRDRELGEELFKLRLRHASGQLESPAQMR
                     RVRRDIARVKTIMRVKEEQKA"
     gene            complement(57570..57992)
                     /gene="rplP"
                     /locus_tag="PLW16_01990"
     CDS             complement(57570..57992)
                     /gene="rplP"
                     /locus_tag="PLW16_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPB69707.1"
                     /translation="MLMPKRVKYRKLQRGRMGGKSTKGAKVTFGEYGLQAMECGWITA
                     RQIEAARVAMTRHVKRGGKIWIRIFPHKSVTKKPAETRMGKGKGAPEEWVAVIRPGMV
                     LYEMEGVDRAVAREAFRLAAHKLPIVTKFLARELSDED"
     gene            complement(58057..58701)
                     /gene="rpsC"
                     /locus_tag="PLW16_01995"
     CDS             complement(58057..58701)
                     /gene="rpsC"
                     /locus_tag="PLW16_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPB69708.1"
                     /translation="MGQKVNPIGFRLGGIKTWGSQWFSEKNYAELLHEDLKVKKFLKE
                     KLYHAGISKIEIERAAQKAKINIYAARPGVIIGKKGAEIEKLKKDLEGITRSEVIINI
                     LEVRKPEVDAQLVAENVASQLERRVAFRRAMKKCVTSALKFGAKGIRISCAGRLGGAE
                     MARSEWYREGRVPLHTLRADIDYGFAEARTAYGAIGVKVLLFHGEVLPGKRSKE"
     gene            complement(58721..59053)
                     /gene="rplV"
                     /locus_tag="PLW16_02000"
     CDS             complement(58721..59053)
                     /gene="rplV"
                     /locus_tag="PLW16_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPB69709.1"
                     /translation="MESRAVAKYVRISPQKVRVVVDLVRGKPVEEAATILAFTRKRAA
                     GMVKKVLKSALANATQNPNIDEKILFVKAVYVDQGPSLKRWRARAQGRAASIRKRMSH
                     ITVILDER"
     gene            complement(59124..59405)
                     /gene="rpsS"
                     /locus_tag="PLW16_02005"
     CDS             complement(59124..59405)
                     /gene="rpsS"
                     /locus_tag="PLW16_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPB69710.1"
                     /translation="MARSIRKGPYIEAKLLDKVRKAEASGSKAVIKTWSRRSTITPEL
                     IGQTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRTFFSHAGDRKSKVKR"
     gene            complement(59409..60227)
                     /gene="rplB"
                     /locus_tag="PLW16_02010"
     CDS             complement(59409..60227)
                     /gene="rplB"
                     /locus_tag="PLW16_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPB69711.1"
                     /translation="MIKKYKPTSPGRRFQTCSDFKDITSATPEKGLVRSLNKTGGRNS
                     NGRVTSRHRGGGHKRKFRLVDFCREKRDVPARVASIEYDPNRSARIALLNYLDGEKRY
                     ILAPDKLKVGDTVVSAERADVKPGNTLPLKNIPLGSLIHNVELKVGRGGQLIRSAGAY
                     GQLMAKEGGYAQVRLPSGEVRKVFIECSATIGQVGNLDHENISIGKAGRARWQGRRPK
                     VRGVAMNPIDHPMGGGEGKSSGGRHPCTPWGVPTKGHKTRTNKGTDKYIVKRRG"
     gene            complement(60266..60553)
                     /gene="rplW"
                     /locus_tag="PLW16_02015"
     CDS             complement(60266..60553)
                     /gene="rplW"
                     /locus_tag="PLW16_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPB69712.1"
                     /translation="MDRYRIIKRAHLTEKSTIAKDEANKYVFEVNRKANKIEIGDAVE
                     KLFKVKVVNVHVLNMTGKKKRLGRIVGQKQNWKKAIVTLAPGNSIELYEGV"
     gene            complement(60553..61176)
                     /gene="rplD"
                     /locus_tag="PLW16_02020"
     CDS             complement(60553..61176)
                     /gene="rplD"
                     /locus_tag="PLW16_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB69713.1"
                     /translation="MPTAAVYNIENEKISEITLSEAVFGASVNESLIYDVVRMQQAAR
                     RRGTAATKGRSDVSGGGKKPWRQKGTGRARAGTTRSPLWKGGGTVFGPQPRSYAFRMP
                     KKMRRLALISALSMKFQEAQMIILRDIPMEEIKTKRFQSVLDRLGLKKALFVIDQASP
                     ILEKSSRNIKDVKMIRSEGINVYDLLKYDRVVLLEPSVKKIEEALLS"
     gene            complement(61203..61829)
                     /gene="rplC"
                     /locus_tag="PLW16_02025"
     CDS             complement(61203..61829)
                     /gene="rplC"
                     /locus_tag="PLW16_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPB69714.1"
                     /translation="MKAGLIGRKLGMTQIFTEDGAAVPVTVIEMEPSVVIQKKTVKTD
                     GYDALQLGYGRIKQKNVTKPLQGHLLKAGKGFFRHLREFRISPEGYEPGQDLTAETFA
                     IGDYVDVVGISKGKGFAGVMKRHGFGGGRATHGSMFHRAPGSIGASAYPSRVFKGTRL
                     PGQLGDVRKTVQNLMVMDVRPDRNVILVKGAVPGGKAGVLLVKKAIKK"
     gene            complement(61926..62234)
                     /gene="rpsJ"
                     /locus_tag="PLW16_02030"
     CDS             complement(61926..62234)
                     /gene="rpsJ"
                     /locus_tag="PLW16_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPB69715.1"
                     /translation="MKDQKIRIRLKAYDYKLLDRSVVDIVETAKRTGARVAGPVPLPT
                     EINKFCVNRSPHVDKKSREQFEIRTHKRLIDIIEPTQATVDALMKLDLSAGVDVEIKL
                     "
     gene            complement(62264..63457)
                     /gene="tuf"
                     /locus_tag="PLW16_02035"
     CDS             complement(62264..63457)
                     /gene="tuf"
                     /locus_tag="PLW16_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPB69716.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKHLSKKGLAEF
                     RSFDSIDSAPEEKERGVTINISHVEYETAKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYIVVYMNKVDLVDDPELLDLVELELRELLTT
                     YEFPGEEIPIIRGSALKALESDDENASDVQSIFKLMDAVDTYVPEPVRDLDKPFLMPI
                     GDVFTISGRGTVVTGRVDRGIVRTGDEVEIVGIRPTFKTVCTGVEMFRKTLDEGRAGD
                     DIGVLLRGTKREDVERGQVVAKPNSITPHTKFEAAVYVLTKEEGGRHTPFFNGYRPQF
                     YFRTTDVTGVTTLPEGVEMVMPGDNVHLEINLITPIAMEEQLRFAIREGGRTVGAGVV
                     SKIIE"
     gene            complement(63541..64011)
                     /gene="rpsG"
                     /locus_tag="PLW16_02040"
     CDS             complement(63541..64011)
                     /gene="rpsG"
                     /locus_tag="PLW16_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPB69717.1"
                     /translation="MPRRREIAKREVLPDPKYHNKLVAKFINSLLKRGKKSTAESIMY
                     GALDIIEKRTKEQPVDVFERAVNNVKPVIEVKSRRVGGSTYQVPTEVAPSRRMALALR
                     WLILHSKERTEKTMREKLAAELIDAANNRGASIKKKEAVHKMAEANKAFAHYRF"
     gene            complement(64026..64397)
                     /gene="rpsL"
                     /locus_tag="PLW16_02045"
     CDS             complement(64026..64397)
                     /gene="rpsL"
                     /locus_tag="PLW16_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPB69718.1"
                     /translation="MPTINQLVRKGREKIQKKAATLALVQSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVSSYIPGIGHNLQEHSVVLVRGGRIKDLPGVRYHIVRGTL
                     DAVGVQDRKQGRSKYGAKKPS"
     gene            complement(64458..68600)
                     /gene="rpoC"
                     /locus_tag="PLW16_02050"
     CDS             complement(64458..68600)
                     /gene="rpoC"
                     /locus_tag="PLW16_02050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB69719.1"
                     /translation="MEDVFSYFEKPKDPIRFNAIKMSIASPEKILSWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHITLATPVAHIWFLKSLPSRIGNVLDLTLKELEKVLYFESWIVLEPKNTPLRKKELL
                     TEEDYIDLKEQYGPDAFEAGIGAEAIRKLLQQIDLEALYEELRTELRTSTSDTKRKKM
                     IKRLKVVEALRKSDNKPEWMILTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPDLRLHQCGLPKKMALELFKPFVYNRLMDKGYV
                     TTVKSAKKLVEKETAEVWDALDEVVREYPVMLNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCTAFNADFDGDQMAVHVPLSIEAQTEARVLMMSTNNILSPANGKPIIIPSQDIV
                     LGIYYLTRADNAVKGEGMVFSGPEEVRSALDAGEVDLHARIKIRVDGQVKDSTVGRIV
                     LYEIIPDTITFDQINKVMNKKELANLIDYCYRCCGDKTTVLLADRLKDLGFRYATRSG
                     SSIAIHNMVVPENKKDIVAKADRDVLKIQRQYMDGLITDGERYNKVIDIWAQATEKIA
                     AEMMSGISSVDVDSAAGKKIESFNPIYMMADSGARGSAVQIRQLAGMRGLMAKPSGEI
                     IETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIITEE
                     DCGTLDGIYVSALMEGGEIIETAGERVLGRVALEDIQDPFTGEVLVRANEGIDEALAE
                     KIDHAGIERVNIRSVLTCRSKNGVCAACYGRDLAHGHLVNIGEAVGIIAAQSIGEPGT
                     QLTMRTFHIGGTAKFEEHSTLEARHDGKIRFMNLNTVLSKTGDLVVMNRNGEVHVLDE
                     QGRGRGKYPIPYGAHLKKSDGSVVKKGNLIAEWDPFSIPILAEVDGTIKYGDIIEGKT
                     MQEQVDEVTGLSRKVIVESRGTDLRPRVSIKDAKGKTAKVPGTNMVARHLLSVGANIV
                     ASEGDAVSAGDIIAKIPRETTKTKDITGGLPRVAELFEARKPKEFAVISEIDGIVSYG
                     KDAKGKRKVVISPDIGEEKEYLIPKGKHVSVQEGDRVRAGEALMDGVHNPHDLLTIKG
                     EKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQMLRRVKIIDPGDTAFIADEQVERH
                     RFQEENERMMAEGGRPATAEALLLGITKASLSTQSFISAASFQETTRVLTEASLAGKT
                     DYLRGLKENVIMGRLIPAGTGLSLYRKLGIKTAVEELPISEIPEESGDESEKSLDSPA
                     LS"
     gene            complement(68656..72747)
                     /gene="rpoB"
                     /locus_tag="PLW16_02055"
     CDS             complement(68656..72747)
                     /gene="rpoB"
                     /locus_tag="PLW16_02055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB69720.1"
                     /translation="MGELRKNFGQIKRILDIPNLIDIQVRSYEKFLQMDVTLEKRGNF
                     GLQGAFRSVFPITDFSGKCSLEFVSYKIGTVRYDVKECIQKGMTYAAPLKIVARLVVF
                     DADRTLEPRPIRDIKEQEIYFGEIPLMTENGTFIVNGTERVIVSQLHRSPGIFFDHDK
                     GKTLASGKLIYSARVIPIRGSWLDLEFDSKDLLYVRIDRRRKMPVTILLKAMGNSTEQ
                     ILNYFYCVEKITVDQDRPQILVEDSLIGEKAPDDVVDAKTGEIVVKKGRKLTRALLKR
                     MGELKIQKIEVNQADLVGKILASDVRDAATGETLLHCNEALSLEGLETVLARGVSELS
                     FIRLDEDMANASIRDTLAMDRIESAEDAIIEIYRRLRPSNPPTPETAHKFFHSLFFDP
                     DTYDLSDVGRAKMNYKLRLDVATDLTVLRNEDILAAVKYLIDLKNGSAECSVDDIDHL
                     GNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLVNAKPVSAVVNEFF
                     GSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPVETP
                     EGPNIGLIVSLSTFARVNEFGFIETPYRLVEDGKVLDDVRYLTAIQEEDHIIAQADWP
                     LDQNGRFTGEMISARKGGDFQTVVPTEVNMMDVSPNQLVSVAAALIPFLEHDDANRAL
                     MGSNMQRQAVPLMKPKVPLVGTGMEAIVARDSGAVVAAKRAGVVESVDASRIVIKSEG
                     PEKDDLDTGVDIYNLIKYQRSNQDTCFNQKPIVNLGDRVRQGQIIADGPATDTGELAL
                     GRNVMVAFMSWGGYNYEDSILVSERVVREDVYTSIHIEEFETMARDTKLGKEEITRDI
                     PNVGEEALRNLDDSGIVRMGVSVKPGDILVGKITPKGETQLSPEEKLLRAIFGEKASE
                     VRDTSLRVPPGVEGTVIDAKIFTRKGAERDHRSQFIEDEALAELEKDRDDEIRIITDS
                     VRKEIRSLIVGKTAAGKLSDPKKRKIVLKKGDEVTEEVLEKIPFELWKEIPLQDEEAL
                     EERIATLLTNLERKVEFVHAYFAGKIEKLKAGDELPPGVIKLVKVYVAIKRKLSVGDK
                     MAGRHGNKGVLSRILPEEDMPYFADGTPVDIVLNPLGVPSRMNVGQILETHLGWAAKG
                     LGDKLGAMLDENVGVKRLKKELKDIYESPEISRLVEGLNEESLLKLVRRLGKGIPMAS
                     PVFDGADEERIKAMLAQAGLPEKGQTVLYDGRTGEPFDQEITVGMIYMLKLHHLVDNK
                     IHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYSLQEFLTVKSDDVAG
                     RTRIYETIVKGEHTLEPGLPESFNVLVKELQSLCLDVDLIEEE"
     gene            complement(72888..73277)
                     /gene="rplL"
                     /locus_tag="PLW16_02060"
     CDS             complement(72888..73277)
                     /gene="rplL"
                     /locus_tag="PLW16_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPB69721.1"
                     /translation="MAEQITKEDVIQFIEKMTVLELSELVKELETRFGVSAAAPVSVA
                     AAAAGPATAAAASEEKTEFDVILTGFGDQKIQVIKEVRAITGLGLKEAKDLVEGVPKP
                     VKEAVSKDEAADIKAKIEKVGGTVEIK"
     gene            complement(73312..73836)
                     /gene="rplJ"
                     /locus_tag="PLW16_02065"
     CDS             complement(73312..73836)
                     /gene="rplJ"
                     /locus_tag="PLW16_02065"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPB69722.1"
                     /translation="MDREQKEQVVVELREKLQSFKLAVLSDYSGMNVEKLTALRNALR
                     KTGTDLKVVKNTLLRIASKDTDMQVFSEQLKGPLALALNDGDVVEATKVLVDFAKKNA
                     ELEIKAGVLNGKVITPEQLAVLAELPSREILLAKLMSVMIGVQTSLVNVLSAVPRGLV
                     QVLGAYQAKQEAAQ"
     gene            complement(74070..74768)
                     /gene="rplA"
                     /locus_tag="PLW16_02070"
     CDS             complement(74070..74768)
                     /gene="rplA"
                     /locus_tag="PLW16_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPB69723.1"
                     /translation="MSSRGKKYLEAKKAIVSGQRYPLREAVEQVVKSAQAKFDETVDT
                     AIRLGVNPAHADQMVRGSVVLPNGLGKTIRVLVFAKGEKEKEAQDAGADFAGAEDLIE
                     KIKGGWLEFDRVIATPDMMGSVGKLGKILGPRGLMPNPKVGTVTFDVGKAVQELKAGK
                     VEFRVEKAGIVHSPVGKVSFGVDKLLENVQAVIEQIVKLKPASSKGTYLKSISLSTTM
                     GPGVKVDPQDVRTI"
     gene            complement(74793..75215)
                     /gene="rplK"
                     /locus_tag="PLW16_02075"
     CDS             complement(74793..75215)
                     /gene="rplK"
                     /locus_tag="PLW16_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPB69724.1"
                     /translation="MAKKVIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     MTQNQEGTVIPVVITVYADRSFTFVTKTPPASVLLKQAAKIAKGAGDPKRDKVGSVTE
                     KQIREIAELKYNDLNAVDMEGAVRIVSGTAKSMGIEII"
     gene            complement(75325..75855)
                     /gene="nusG"
                     /locus_tag="PLW16_02080"
     CDS             complement(75325..75855)
                     /gene="nusG"
                     /locus_tag="PLW16_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPB69725.1"
                     /translation="MALRWYVVHTYSGFENRVKMSLQERIESAGMQDFFSDVLIPEED
                     IVELISGEKKTSKRKFFPGYILVRMEMNDETWHIVKDTPKVTGFIGSKDKPSPIPDKD
                     VENLRARIDEGTLKPKPKFKFDEGDHVRIIDGPFTNFDGMVDEVKPEKGKLRVIVSIF
                     GRSTPVELDFIQVVLN"
     gene            complement(75860..76054)
                     /gene="secE"
                     /locus_tag="PLW16_02085"
     CDS             complement(75860..76054)
                     /gene="secE"
                     /locus_tag="PLW16_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPB69726.1"
                     /translation="MKLIIAQVGKFLKEARVELKKVTWPTPKQTLASTSVVILVSAVV
                     SLFLGLVDFGLTKIIKLVLG"
BASE COUNT        13649 a        24913 c        22571 g        15019 t
ORIGIN      
        1 ccccacaccg gatttgaagt ccgggaggcc caccagtgac cttggcactt ccttgatgtt
       61 cgttctcaat ccttcagata ttcgggctcc gccatccctt ccagggaacc gtcgatgagc
      121 tggacggtga cctcttcacc cgcccgcgcg tgcgttaccg cctccggaat aacgatcagg
      181 ccgttggcca gcaccatcga tttcaggatt ccggagccct gttctcccgt ggtggaggca
      241 tggtaccgcc cgtccctgaa ttcgacccgc gcgcgcagga agtttctcgc ccctgcgtgc
      301 ttctggacgt cttcgtccag gcacgcctga atgatccggc ggaagaggtt ccggcgtccc
      361 atcatcttga gcagggccgg acggacgaac tgctcgaagg agaccatgga cgagacgggg
      421 ttgccgggca ggccgaacag cggaacgccg ccgagagtcc cgaaagcaag cggcttgccc
      481 ggccgcatgg cgacctgcca gaactgcatc cggttgccca cctctttcat gatctccttg
      541 acgagatcgt agtcgcccac ggagacgccc cccgaagaca ggatgatgtc cgcgcgcagg
      601 gcggcgttga acttcgcgat caggtcctcg cgggtgtccc tcgcgatgcc gatctgcagc
      661 ggcaccgccc cgcagtccag gacctgggcc gcaagcgaat agctgttgct gctgacgatc
      721 ttcccctccg tcaggaaggc ttcgtcgaca tccacgagtt cgttgccggt cgccagaacc
      781 gccacggtgg gccgctgatg aacgtcgatg acggcgcggc cgacggaggc cagcatgccg
      841 atccgggccg gcgtcaggac atcccccttc cggacgatgc gctccccggg ccggacatcc
      901 tccccggcgc gacggatgtc caggccggcc gaggccgcgg cgaagatccg aacccggctc
      961 ccgtccttct cggtctcctc catcttcatg acggcgtcgg cgccatccgg gatgggggcg
     1021 ccggtcatga tccgtgccgc ggttccggcg acgacggtct tctccgggac tttcccggcc
     1081 gggatgtcct ccacgacctc gaggatcacc ggggattccc gcgtggcccc gagcgtgtcc
     1141 tcccaccgga gggcgtagcc gtccatggcg gagttgtccc ggggcgggat ggcgcggcgg
     1201 gcatcgacat cctccgcggc cacccggccg agggcggcga gcaagctcac cctttccgac
     1261 cccagggggc tgatggcgga cagaatctgt tccagggcct gttgaacaga gatcatcggt
     1321 ttcgatcctt ggtttggtgg ctgaacgcta acgtaaatcc gcacgcttgt caatatcggg
     1381 atgcccggcc ccctccggat ttgtccttga agtaattatg tttctgttgt agcaaatgag
     1441 gctatttatc cggggaatgc gtgcatcttt gtcccgcccc cgggaaagga cgaaccggtc
     1501 atgacggaca tacaggcatg gcagaggaag gtacgccggc tgctgaggga gcggcgggcg
     1561 attctcctcg cccactacta tcagcgggac gagatccagg agattgccga tctccggggc
     1621 gactccctgg ccctcagcat cgaggcggcc cggacggacg ccccggtcat cgtctttgcc
     1681 ggggtgcatt tcatggccga gagcgcggcc atcatctccc cggagcgcac cgtgctcctc
     1741 ccccgcccgg acgccggctg cgccttggcg gactggatca cggcggccca attgcaaaag
     1801 gtccgggaag ccgaccccga tctgctcgtc gtcacctaca tcaactcttc ggccgcggtc
     1861 aaggccctga gcgacatctg ctgcacctcg gccaacgcgg tccgcgtggt gaactccctg
     1921 ccggaggggc ggaaaatcct gatggcaccg gacggcaacc tcgcccgtta tgcggcccgc
     1981 catacgacgc gggacatcat cccctggaac gggcattgtc ccgtgcacca tgacatcacc
     2041 gtggacgaag tccggcgcgc caaggtggcc tggcctgggg cgcctttcgc cgcacatccc
     2101 gagtgcaatc ccgccgtcct cgacgaggcg gatttcgtcg gcagcacggc ggggatcctc
     2161 cgtttcgccc gggaaaccga tgcgcaaacg gtcctgatcg gcacggaacg gggaatcttc
     2221 cacacgttgc gcgaagaaag cccgggaaag cggttcgttc cggtctccga aaggcttctg
     2281 tgtgaaacca tgaaatacac gaccctgccg gatatcgtcg cgtccctgga gacgatgtcg
     2341 cccgtgattt ccgttccgga agccgtccgg gttccggcaa agcgcgcgct ggaccacatg
     2401 ctggcccttg gatgaggaga ggccggaggt gagccgactg gtgcgcacga tcatagcaga
     2461 cagacacgcc ttgcgcgatg ccgccctggc cgggctggtg gaccgggtcg cccgcggggc
     2521 gtccggcctc cgggttcagg gggtgtcggg aagcgcccgg gccctcctgg ccgcggtcct
     2581 tttccggcgc ctcggcaaac ccctggcggt ggtcaccccg acggagggcg aggcgcgcgg
     2641 cctgcaccgg gacatggcct ttttcctcgg ggaagacgac gcgcttctct atcccgaagg
     2701 cgaaaccgtg gcaacggaca tcctaaccgc ccagagcgag tccgagcggg accggatcca
     2761 cgtcctcgac cgtctcctcg acggcgcgcc ggtgtcggtc gtcgcctccc tgccgtcgat
     2821 gctgaggagg gttctcccgc gggacgtcct gcgcgccagc cggatcgccc tctcgatggg
     2881 ggatgcggcg gaccgggacg acctggcgcg gcggcttctc gccgggggct acgagcgggt
     2941 aacgctggtg gcggaggccg gggagttcag cctgcgcgga catatcctgg acgtctttcc
     3001 ccccggcgcc caccacccgg ttcgtctgga gttcgtgggg gacgagatcg agtccctccg
     3061 cgccttcgac gccgcctcgc agcgctcgat cggggaaatc ctgtcctgca cgcttatccc
     3121 ggcacgcgag gcgatcctga cggaggaacg gatcgggcgg gcgatccgca atgtccgacg
     3181 ccgcgcccag acgctggaac tgccccgccc ggtatgggaa tccctttcgg acgccatcgc
     3241 cgaaggacgc accgcgcagc ttcccgcccg gctgctgccc ctgttctacg agaacctccc
     3301 cgggacggaa gacccgacgg ccgcggaggg tctgggcacg ctcttcgact acctgccggc
     3361 cgaggcgctg ttcgttctcg acaatcccgc cgccctccgt cagtcccagg aggaattcgc
     3421 caacgagttg gacgggctga tcctgaaggc ggagcgggag gagcggttct ttctggaacg
     3481 ggagcagacg cacctgtccg gcccggacgg atgggcggcg attcaagccc ggccacattt
     3541 gttggtcggc gggctgacgc tgaccgccgg cgatgcgaaa cccgccgagg ggggaacgac
     3601 ggcggaaacg gccgtcacgc tgtccacgga tcgggagacc acccggctcg aaggcgggcg
     3661 ggtgtccgtc gcggaagagg gcctcctgaa acctctggcc gagcgcatcc ggcaatggat
     3721 cgcgtccgga taccgggtcc tcttcgtctg cgcgggaccg gaaagccggg accggatcgg
     3781 tcaccttctg gcccagtacg accttcccgc gacgcgggag gaggcgtttt ccctggagga
     3841 caccgtcagc cagcgcggcc gagggcgcct ggggctggtg gagggtcgcc tgaccggcgg
     3901 attccattgg ccgggcctga agctcgccgt ggtcagcgag gaggaaatct tcggccggaa
     3961 gcattcccgc cgccggcccc ggccggtgcg ggagggatat tttctcaagt cgttcggcga
     4021 gctcaacgag ggggaccccg tggtgcacac ggatcacggc atcggcgtct accgcgggct
     4081 cacgaggctc gccgtgggaa atatcgagaa cgattttctc ctgatcgagt accaggaggg
     4141 ggacaggctc tacctgcccg tggatcgtct cgatcggatc cagcgctata tcggctccga
     4201 cgggaacgat ccccggctcg accgtctggg cggccagtcc tgggagaccc tgaagtcccg
     4261 ggtgaagaaa tccatccggg aggtggccga ggaactggtt tccatctacg ccgcccgcga
     4321 ggtcatggaa cgggagaact tctccccgcc cgaccggtcg tacgaggaat tcgcctccgc
     4381 cttccccttc acggaaaccc cggatcaggt ccgggcgatc gaggatgtcc atctcgacat
     4441 gaacgccccc aagcccatgg atcgcctcat ctgcggcgac gccggcttcg ggaagaccga
     4501 ggtggccctg cgggcctcct tccgggcggt catggacggc cgccaagtgg ccgtgctcgt
     4561 ccccacgacg atcctggccg agcagcacta tcagacgttt ctggagcggt ttcgggatta
     4621 tcccgtccag gtggcctgcc tgaaccgctt ccgctcccgg gtccagcagg ctgagatcgt
     4681 ggcggggatc catcggggaa ccgtggacat cgtcatcggc acccatcgcc tcctgcagaa
     4741 ggatgttgca ttcaaaaacc tggggctggt catcatcgac gaggagcagc gattcggcgt
     4801 cctccacaag gaaaaactca agaagctgcg gaccctggtg gatgtgctga ccctgacggc
     4861 gaccccgatt ccccggacgc tgcacctgtc cctggtcggc atccgggacc tgtcgatcat
     4921 caactcgccg ccggaggacc ggcagccgat caagacctac ctcgccgagt tcgacgagga
     4981 catgattcag aaggccatcc gtcaggaact gctccgcggc ggacaggtgt tcttcctcca
     5041 cgaccgggtc cgctcgctca acacgatggc gcgcctcgtg gagaagctcg tccccgaggc
     5101 ccgggtggag gtggtgcacg gccagatgaa aagcacggac atcgaacgca tcatggccgc
     5161 cttcgtccgc cgggacttcg acgtcctggt ctgcacgacg atcatcggcg ccggactgga
     5221 catccccacg gccaacacga tgatcatcaa ccgcgcggac cgcttcggcc tggcacagct
     5281 ctaccaaatc cgcggccggg tcggccgcgc ccgggacgag gcctatgcct acctcttcgt
     5341 gccccggggc gccctgctct cacccgaggc ccggcggcgt ctggaggtga tcctcgaatt
     5401 caccgaaccc ggcagcgggt tccgcatcgc cgcgggggac ctggatatcc gcggggccgg
     5461 aaacctgctg ggcatgtccc agtccggcca tatcgccgcc gtcggctacg agctctacac
     5521 ggaactcatg gaaaagacga tccgggagat caagggggag ggcccgcccg aggagcgcgt
     5581 gcggccggag attcacctgg gggtgccggc ctatattccc tccgattaca tgagcgacga
     5641 gcatcagcgg ctcgtcacct acaagcggat ttccctggcc gccgacgagg acaccctggc
     5701 ggagatccgc gcggaactgc tcgactgtta cggattcatc cccggagagg tggacaatct
     5761 cctcgaggtc atccggatcc ggaacgccct cacggatctg aagggacgca agatgggata
     5821 cgatgaaaaa cagcttttca ttcatttttc cccggacagc cccgtcgatc cggcccggat
     5881 cagcgacctc gcgaagctca tgaaccttcc gccgccccgg gagaagccgg tgcgaacgct
     5941 cgcccgatcc ctgagaaagc ccgcggccgc gcgggaaaca ccgcccggca tccggctgac
     6001 gccggattac cgcctgtatg ttccgcttcc cggcctcaag ggggacgggg tcctcgcggg
     6061 ggcccggcgc ctgatcgacg ccctcggcac cgggaaaccc gatgcgtgaa aaaccgaccc
     6121 ggccgaatgg cgcctcgaac ggccaggcgg ccccttcttc gacgaaagga gacagactct
     6181 tgaaagccca tcaaatcgtt ctgctgtttc tggtttgcct gttcgcgtca tccgcggcgg
     6241 ccgcaaccag cgtcgtggag cggatcgtcg ccgtggtcaa cgacgagatc atcacctcct
     6301 atgaactgaa cagcgccttc gaacccatgg agaagcggat cgaagacacc tacaaagggc
     6361 ccaacaagga acggctcctc caggagacgc ggatggatct gctcaagaga atgatcgaca
     6421 agcttctgct cgaacaggag gcgaagaaaa cggggatcgt cgtcaaggat gaggaggtca
     6481 tggaaacgat ccgcgacatg gcccggcggc agaacgtttc gctggacgcc ctgaaggagg
     6541 ccctcgccaa ggaaggaacg accctggagg cgtaccggaa cgagatccgc gaacagatgg
     6601 tccggatgcg catcctgagg cgcgacgtcc gatcgaaaat cgccatcagc gaagaggaaa
     6661 tcggcgagta ctacctgaag catcgcgaag tgtacgaagg gaaagaggcc gtgcacatca
     6721 agcagatcct gctcccctat tcccccaacc tggacgagga aggcaaaaag aagctgcggg
     6781 agcaggcgga gaccatccgg cagcggatca tggcggggga gcctttcgac ctgatggcgg
     6841 tcacctattc ccagggaccg gcggcggccc agggcggcga cctgggattc atcgagaagg
     6901 ggctgatgct gccggaggtc gatcgggtcg ccttccgcct caagcaagat gaggtcagcg
     6961 agatcatcga atcctccctg gggctgcacc tcttgaagct ggtggacaag cgcggcgcgg
     7021 gcgtcaagcc catcgaggct gtgcgtcaag agatcgtgga tcggctggaa gagcagaaac
     7081 tggaagtgaa gtatggagac tggatcgaag ggctgcgcaa acggtcccac attgaaatcc
     7141 gtctgtagag cgggcaggcg gccgggcggt tctccgcccg cggatgggtg gcgcccatga
     7201 actggatccg catcaagggg gcgtcccagc acaatctgaa gaacgtcagc ctcgacatcc
     7261 cgcgggaccg gctcgtcgtc atcacgggcg tctcgggctc gggaaaatcg tcgctcgcct
     7321 tcgacacgat ctacgccgag ggacagcggc gctacgtcga atccctgtcc acgtacgcgc
     7381 gccagttcat cggacagatg gacaagccgg acgtggagtc catcgaaggc ctgtcgcccg
     7441 ccatcgccat cgaacagcgt gcggcgagcc acaatccccg gtccaccgtc ggcacggtga
     7501 cggagatcta cgattatctc cgcctgctgt ttgcacgcat cggcgtcccc cactgttatc
     7561 agtgcgggcg cgagatcctt gcccagacga tcgacatgat gatcgaccac gtcctcggcc
     7621 tgcccgagaa gacgcgggtc aacctgctgg cccccatcgt ccgcgggaaa aaaggggagt
     7681 tccagaaaga cctgaagaaa ctgctcaagg acgggttcgt ccgcgtccgc gtggacggcg
     7741 aactgcggga actcgccgac gagatcgtgc tcgacaagaa caagcgccac gatatcgatt
     7801 gtctcgtgga ccggctcgtc gtcaaggagg ggatccgcaa gcgcctgcgc gactccctgg
     7861 agacggcggc aggcctgacg gacggcctcg tccgggtgga agtcgtcggg ggagaggaga
     7921 tcctgttcag cgagaaatat gcctgtccgg actgcgggat cagcctgccg gagctggcgc
     7981 cccggatgtt ctcgttcaac accccgtacg gagcctgccc ggactgcgga gggctggggt
     8041 ccaagatgta ttttgacgaa gtcctggtca tccccgaccc gtcgctttcg atccgggaag
     8101 gcgccatcgc gccctgggcc gggcggaatt cggtcttctt cttccagcgg ctggacgccc
     8161 tggcccagca ctaccagttc gacatcaaca cccccttcaa ccgcctgccg gcgaacatcc
     8221 gcgaggcgct cctgtacggc tccggcaagg aaaagatcaa gttctactgg gaccggcgcg
     8281 ggcggcggtt cttttacacc aaggccttcg aggggatcat caacacgctg gaccggcgct
     8341 accgggagac cacgtccagc gccgtccgtc tggagctcat gaagtacatc agcctgcgcg
     8401 aatgtccggc ttgcaggggc gccaggctga aacaggagag cctgtccgtc accgtctccg
     8461 gccggaacat cttcgacgtc tgccgcctgt cgatccggga gtgccaggcg tggttcgaca
     8521 cccttcccct gacgccccag gaaacgctca tcgcggaacg gatcctgaag gagatccggg
     8581 agcgcctgcg ctttctgatc gacgtaggcg tggactacct gagcctggag cggtccgcgg
     8641 acagcctctc cggcggcgag ggccagcgca tccgtctggc gacgcagatc ggctcgggcc
     8701 tggtgggggt cctctacgtc ctggacgaac cgacggtcgg tctgcaccaa cgcgacaacg
     8761 tccgactgat ccacacgctc aagcgcctgc gcgacctcgg gaacacggtg ctcgtcgtcg
     8821 agcacgacgc ggacatgatg ctgtcgtccg atcacatcat cgacatgggg cccggggccg
     8881 gccaggaggg gggcgaggtc gtcgtccagg ggtcgcccgc ggaggtgtgc cgctacggcc
     8941 cctcgctgac gggccagtac ctctccgggc gggtttcgat cccccttccg tcatcccgcc
     9001 gcccccggcc ggaccggttc atcgtcctcg agggggcgta cgagcacaac ctaaaggaca
     9061 tcgacatccg catcccggtc ggcgtgttca ccgcggtcac cggggtctcc ggctcgggaa
     9121 agagcacgct ggtcatgcgg accctgcaca agatcctgga gcgcaagctc tcccggcagc
     9181 ggggccgggt cgggaagatc cggcgcatcg tcgatctggg cggcatcgag cgggtcatca
     9241 tgatcaacca gcagcccatc ggccggacgc cgcggtccaa tccggtgacc tataccggga
     9301 tcttctcccc gatccgggag ctcttcaccg gactgcccga cgcccggatg aggggctaca
     9361 agccggggcg gttcagcttc aacgtcaagg ggggacggtg cgaggcctgc gagggcaacg
     9421 gcctgatcaa gattgagatg cacttcctgc ccgacgtcta cgtcacctgc gacgtctgcc
     9481 acggccggcg cttcaacccg gacaccctgg acatccgcta caagggcagg aacatcgccg
     9541 aggtcctgga tttgacggtc ctgcaggcgc tgagcttttt cgaaaacatc ccgtccatcc
     9601 ggacccggct ccagctcctg tacgacgtcg gcctgggcta cctccgcctg gggcagtcgg
     9661 ccacgaccct ctccggcggg gaggcccagc ggatcaagct ctcccgcgag ctgggcaagc
     9721 gggccaacag caacaccctc tacatcctgg acgaacccac catcggcctg cacttcgccg
     9781 acatccagaa gctcctggac gtcctgatgc gcctggtcga catgggcaac accgtggtcg
     9841 tcatcgaaca caacctcgac gtcgtcaagt ccgccgacca catcatcgat ctgggcccgg
     9901 agggcggccc cggcgggggt tggatcgtcg ccgaggggac gcccgaggag gtggccgcct
     9961 gcgaggagtc cctgaccggc cggttcctca agccgatcct ttcacgaacg gggccggcct
    10021 agcccccctg cggcgacgga cgtctttttc aggattttcc gatataggta aaggccttcg
    10081 gaaccggacg ggcgtacacc tggccgtgcg tctcgtcatc gaaggcgccg gcctgccgaa
    10141 gggcctcacc gatggccggg gtctgctccg gggagacgat catgttgcag cactcgcgag
    10201 cgggggacag gcggctcagg ggggactgcc ggggacggac atgcctttgc cgcccgatgg
    10261 tcgcccccgc ggctcccgca gccatggcgg ccttgacgag ctccggcccc cgtccctcgt
    10321 tgcagagcat cgtcagatcg gtcaggtcgc gcagataggc cttccccgcg tcttcgctct
    10381 ccgcggtccc ggaacggcgc ctccatttgg tcccgccctt gagttcatcg atgacggaga
    10441 caatctgctc aatgctggcg gcgtgccggg tgacgccccg cttgattttc gtgttgacaa
    10501 tgccccggtt gaggggtgcg caatacagga agcccttgcc gggctggtcc aggtcgccca
    10561 cgtcgatcat caggttcatg aggccctccg catcgtgccg gtccgtaacg atgtggacca
    10621 cctccttctc ggcgggaatc gtgatccgga ggagccccat cttgtcgcgg acgccggtgc
    10681 cctgtccgaa ggtgatggcc ggcacgcacg ttccggtgtc gagggagatc atggccatcc
    10741 ggtcgccctc gccgcgctgc gcgatgcagc acacgagcgc cagatcggac aggaggggaa
    10801 tgtcgctcgc caccgtaaaa tcggccgtcc ggttttcagc gcagagctca tgggcccaga
    10861 gcagttcgac ggtctcgctg aaggccatgc cccgtcccgc aatgccgagc cttcctgcgt
    10921 gaaggacaag cttcagcgcc gcgtcctcca gggcggggtc cacgaggaag gtcaggattt
    10981 cgatcgggtc ttcgtcgatg acggtgcgct ccgaggcccc aaaaagccac ttcttgtcgt
    11041 gcaggacgtc ggagcggccc acggcaagat gtgcgtcgac gattccgtgt tcggcgagcg
    11101 cctcgatgac gcctaccgcc tcccctcggt tgacgattcc ggtaatcttg gcaacctgct
    11161 tccggcgctc catcatgggg tcgtccctcc ttttcccgac ggcacggacg cctgcgcggc
    11221 cctgcgcttc atcgtcaccc aaaggccgac cacgagcacc gaaaggatgg gacagacgga
    11281 ggccatcgcc agaatgccga atccctcgac caccccgacc tgggtgctga tcccgagacc
    11341 catcgcgagc accagcggca cggtgatcgg cccggtggtc acaccggcgc tgtcccaccc
    11401 gatgtttacg aactcctcgg tcgaaaaggc cgtgacgaac aggagcaaaa gatagggcgg
    11461 cgccaggagc cagatcagcg gaaggtccca gacgatcttg gccacgccga tcgcaagtcc
    11521 cgctcccact ccgatggcaa cggtctgcat gagcagggac tttttgaacg cgccgacggt
    11581 gagatcttcg acggtggccc ccagggcgtt aagggcgggt tcggccaggg tcgccgcgta
    11641 ccccagaatg aaggcgaaca ggagcaccac gagaatgccc ccgaggccgt ccgagcgacc
    11701 gaagagcggc ccccgggtcg gaacgtacgt gtactggcgc gtctccgggt tatactgccg
    11761 ctcgttgaac ggcagcggca ggagccgatc cttttccttc agataaaaga aacggctctc
    11821 ctcccccttt tgcgtcaggg cggtctgcac gacccccggg tcgaagtcgt ggatcgtctt
    11881 cgtctggtcg gtcaactcga cggccttgaa ggcggagggg atcttcccac cgacctggcc
    11941 cccgagcttc gccagcccca gctcgatgcc gatgttgaac agccccatcc cgatgacggc
    12001 aaacacaagc cccaggagga tttcatcggc ccgcggcagc ttctcccgaa gcacaagggt
    12061 cagcacgaga atgaaaaaca gcgacagcgg gataatggcc tgggccgcgg cgcgcgtgtt
    12121 gcgcgccagg aggtcgaaca catcgagacg ctccgggagg gcggaggcgg aggcggcgta
    12181 ccgggtggac gcctcgatgt ccccgaacac cgcgacctgc tgctcccttg ttccttggga
    12241 tgccgcccat ttcttcaggg catccgcttc ggggccgaag acccgctccc gcaccttcgc
    12301 ctcggcggac gcctccctga gaaaagcgag catcccgtcc cgatcccccc cgaagaacgc
    12361 caactgattc tccggggcgg cgttcaggaa cgcatagccg gccatttcat ccggatggct
    12421 gaagagggcc tgtgtcgcct ggcgattttc gagcttgaaa aaatcggcct cgctcatggg
    12481 ctggggaacc gagcccacaa agagggttcc cagggacatc acggtcagaa tcgggaaaag
    12541 cgaggcgagc gtcaccacgc cgaagcccgc cgatcccgat cccgcgcttc ccacaacgcg
    12601 gcagaccccg atgcccaggg ccaacaccag gggcacggtg accggaccgg tggtcacccc
    12661 gccgcaatcc cacgcgagac cggtgagaaa catcatgttc ttgtcgaaat aggcccagac
    12721 ggagaagacg accagcacgg ggatgagcag atagatgaag ggcttgaggg accagttgta
    12781 cagaaacctc agcatgcccg cgaccacggc gatgccgacc ccgatgccca cggcgtaaat
    12841 caggaagtcc gggtatcgat tcagcagcag gaaaagcagc ggtgcgtccc aggccttgac
    12901 cgaggcgccg gcggccttga ggaccccgat ggcgggctcc gcaaaggtgg cgccgacgcc
    12961 gagaatgaac gagaaaacca ggatgaccgg aaggacggac ttttgcggga gcttgaggcc
    13021 gatgacttcg cccaggggca tgagccccag gaccaggccc tccatgaaga acgtgaggcc
    13081 gacgatgacg agcgccaggc cggcggcaat gacggtggcc ctggccacgg ggatccccag
    13141 gatcagggtc tgaaacagga ccaggtagac gatgatgagg gcgacggcct tgatctgcgc
    13201 catcagccgg cttttgacgt acggcgccag catgccgacg gcctgtctga aaccgacccg
    13261 gatccttccg cccgcctgct gtcgaaccgt catgcgccct cccccgctgc ttccgtccgc
    13321 cgggacattc agacgcttcc ccggctgctt caaaggtttt tcccctcatc cggcccatcg
    13381 cgaacgggcg cgcggggttc tcgggcagaa gatcgagacc ccgttccgtt cgccgccgag
    13441 ccgcctgcac atccgggatt tctccaaaaa taggcgttct ttcgttcagt taggcaaaac
    13501 aacctaacgg attccgaccg ccttgtcaag cgccgcgggg ggccggtgcc ggggccgttc
    13561 ccgcgggagt ccgcatcccc ggaggttggg caggacaatc ccggcgatga tgagccccat
    13621 ccccgcgatt tggacgaaaa cgatgttctc ccccaggacg agccaggcca tgaccacggt
    13681 cgcgaccggc tcgaaggtcg agaagatgga catccgggcg ctgccgagct cgcggatggc
    13741 aaggaactgg aaggtcacgc ccagggccgt ggggacgagg ccgatcgcga gcgcgaggag
    13801 cagctggcgc ggcgccaggt gcacgatgtc gagcggattg ttgacgaaaa gaaacggaac
    13861 cgccgtgaac aggatcacat agaaggtgac tgtcaggaac tcttccccct tgaggaagat
    13921 ctgggacagg atgagataca ccgaaaaggt caaggtggtc cccatcgcga acagcagtcc
    13981 ggtcacgttc agggccttga ggaaggcgtc atagaagact agggcgcatc cccccatcag
    14041 aagcgccagg gaaaggccga tcgtccggtc cgccttcatc cggaaaacgg cgagagacag
    14101 gagcgtgacg atgacggggt agaggtagag gatcagggcg ttcgtcgacg cggggatgta
    14161 attgagggcc ttgaagaagc aggtgctctg ccaaccgtgg aggaccaccc cgaggaaggc
    14221 ggtcttggcc agggtggccg gacggatcct gagcaggcgg ggatggcgga tcaggaggta
    14281 aaggccgaag accagggtcg ccgtcagaaa gcggtactgc aggagcgcca tgtccttcag
    14341 gccggtctgg taggccagct tgatcaagat ggccagcatg ccgaggcaga acgcggaaac
    14401 gagcgaatag atcaggccct tgagcatgcc tctcttccgg tatgggcgat gggggggcgt
    14461 gcggcgtttc ccgggatggg cggatccggg ctccgggaag gccacgtccc gcgccgccgt
    14521 ttataggcaa ttcccgccgg tttgtaaagg gatcattggg gcccccgccc ggcctttttt
    14581 tctttgacaa atccggggcg caattttaga ttcaaccgta gaatccgttg acacccggaa
    14641 gagggggaga ggccatggga gagatcaccg agaaggaccg ggcgatggcc cggcgctgcg
    14701 tcgagtgccc ggtctgccgc cgggcgcggc gccggcaggc gggtgtggcg ttctggctcg
    14761 tgaagaacgt cgaggaggga ctctgcccgt tctgccgggc ctacgagaag gtctacgggc
    14821 gcaaggctca tgagccggca ccctgagaat gcacgggaca atccgggaat gggtccgacg
    14881 gaaggggtag agacgatgct caagcacgtg gtgctcatga agttcaagac cggggtcggc
    14941 gaaaccgatg tggccgatct cgaacgcggg ctggcggggc tgcccgccgt gatcccggag
    15001 atccggggct acgacttcgg ccgggaccgc cggccggagc gaacgttcga ttttgcgctc
    15061 gtctccacgt tcgacgacga gaccgccctg gagcgctaca agccccaccc gcgccaccag
    15121 gaggtcctgg ccagggtcaa ggccctgtgc gacgagctcc gggtgatcga tttcacgtat
    15181 tgatccgctc cggggtgggc aggaaagagg cggggccgtc cgtccgctgt ttcgccggcg
    15241 ggcctcaaac gtaaaggacg tttttgcgga tgtagtggcg aacggcgtcc gggacaaggt
    15301 agtggatgga ttggcccgca cggacccggg cgcggatccg cgaggccgcg atgtcgagga
    15361 aggtgacctc ccggaaatag atgaactggc cggtgggtcc ccgatagccc cggcacgcct
    15421 cgtcgtagac gaagcgggcg gtgaagtccg cgggtaggat gccgtcgagc ccccggtttt
    15481 catagccggg gcgtgtcatg acgacgaaat ggcacaggac gaggaggttc tcccagtcct
    15541 tccacgtccg gatggcgtgg aaggcgtcct ggccgacgat aaaatacagt tccaggtcct
    15601 tcaggtactt gccgaggaag gactcgaccg tctccaccga aaacgatacc cccccccgct
    15661 cgtgctcaat gtccgaaaac gaaaaggacg gattgtcctc gatggcaagc tcgaccatct
    15721 gctcgcggtg gtagaaggaa gtgatgtcgg cctccagttt atgcgggggg cgcgacgcgg
    15781 ggacgaagat gatgcggttc aggtcgaaca tctcccggat ctcctcggcg cacctcagat
    15841 ggccgaaatg gatcgggtcg tacgtgccgc ccaggagtcc ccatttcatg ttctcacctg
    15901 tccgtttccg tagatgatga atttggtcgt cgtcagttct tccagcccca tgggcccgaa
    15961 ggcgtgcagt ttggtggtgc tgatgccgat ctccgcccca agtcccagct cgaagccgtc
    16021 gctgaagcgg gtcgaggcgt tgacgagaac cgtggaggag ttgacctcgt tcaggaaccg
    16081 ctgcgcccgc gtgtagttct ccgtcaggat ggactccgtg tggagtgagc cgtagcggcg
    16141 gatgtgggcg atggcctcgt ccaggtcggc caccacgcgg atcgagagga tcagatccag
    16201 gtattcccgg gaccagtcgt cctccgtggc cggcgccaga tcgggcagga gagcgcgcgc
    16261 ctgggggcag cccttgagcg ccacgcctgc ttcccgtaat cgctccgcca cgacgggcag
    16321 gaaggcgggc gcaaccgccg catggatcag gagcgtctcc atggcgttgc agacaccggg
    16381 gcgctgcgtt tttgcattca ggcagatcgg aaccgcccgg tcgagatcgg catccgcgtc
    16441 gacgaagatg tggcagacgc ccttgtagtg cttgatcacc gggatcctcg actgactcac
    16501 cacggcgcgg atcaggtctt ccccaccccg cgggatgatc agatcaatcc attcgtcgag
    16561 ctgaagcatc tcatggacgg cctcgcggtc ggtgagcgga atcgcctgga tggcggctgt
    16621 cgggagaccg tggtcgcgaa ggacgcccgt caggcaggcg gcaatggcca agttggaatg
    16681 gatggcctcc gatccgcccc gcaggatgac ggcgttcccc gacttcagac acagcgccgc
    16741 ggcgtcggcg gtgacgttcg gtcgcgattc gtagatgatc ccgatgacgc cgagggggat
    16801 gcgcatccgg ccgaccagga gcccgttggg ccgccgccac atgccggtga ccttgcccac
    16861 cggatcggtg agggccgcca cttcgcggag accctgggcg atccccgcaa gtgtctgctc
    16921 cttcagggtc aggcggtcga tcatggccgc gggcaggcct ttctcgcgag ccgcttcgag
    16981 atcccgggcg ttctccgcga tcaggtgcgc ctgccgggcc agcagcgcgt cggccatcgc
    17041 gaggagagcc gcattcttcc ggtcggtggg gacatgggcc aggctgtcgg cggcctgcct
    17101 agcctctcgg gcgattcgaa cgatctggtc gtggagattc atgaacgggt cctttgtgcg
    17161 agattcgcgg cgtgatcccg acgcggagat ccggtctcaa atcgctcccg tcgggcggcg
    17221 gacccactct acaaaacacc ctttcacgtg tcaaggccaa ttgcggccgc ggacgcaaag
    17281 gcgacgcttg ccaagttccg gcaggtctgt taaggtcgcg gccgatcggg acggcggaaa
    17341 cgagggggag gacaaaattg cgatggattt tctgcggatt aaggcgggac cgagggcgta
    17401 tgagcagctc atggacggcg gcggcgtctg ggaccgcatc tcgacctatt tcgggccggc
    17461 ggcgggtcca cgctggctgg tcgcccaagg gttcgatctc acgctgatga aggagggtct
    17521 gctcggtcgc gccctgccgg tgtggctcgt cggctcctcc gcgggcgcct ggcgcttcgc
    17581 cgcctggctg cagcccgaac cggagaagag ctacgcggcg ttgcgggagg cctacacagg
    17641 ggtgacttat gcccggggcg acacgccgga tacgattctc gacaagctcc tgaacgtcat
    17701 caacgcctat atcgaggacg acgcgctgcc cttcgccctg aaccacaagc gctaccgcct
    17761 ggccatcctc acggcccgca cgcgtcacct ggcggcctcc gagatcggct ggatccagaa
    17821 ggggggcctg gccctgtgcc tgctcggcaa catgatcagc cgtacgaatc tcgcccgctt
    17881 cgccgagcgg gttgtgttct tccacggacc ccgcccgccc gccttctgca cgcacccgga
    17941 gttccagggc cgtttcattg gtctttctcc gaacaatttc aaacaggcgg tgatggcctc
    18001 cggcgcgatt ccgctggtcg tcgccggcgt ccgggacatc ttcggcgcac cccggggggt
    18061 ttaccgcgac gggggactga tcgactatca cctgagccat gattactcgt cgcggccgga
    18121 cgatctcgtc ctgttcttcc atcaccagga gcgcatcgtc cccggctggc tcgacaagaa
    18181 gcttccccgc cgtcagccca ccccggaagc cctggagcgg gtcctgatgg tgtttccatc
    18241 cgaggccttc ctcgcgcggc ttccctacgg aaaggtgccc gaccgggacg acttcctgac
    18301 cttcatcaat gaaccggcgg agcggatccg ctattggaac cgttccgccg agttggcggc
    18361 gcctctgggg gagatgttcc tcgaactggt ggagagcggc cggatccgcc ggttgctgga
    18421 gcgtttctga accgaaggcg cggggccggc gaaacgagag gagcccctcc cggacaagcg
    18481 gcgccccgcg gggccgcgga aaggagattt catgaaactc aagggggtga ttacggtcgt
    18541 ccgcgtcccc ttcctgatcc tcgcgctgat cctggggatt ctgggggctg cggtggcctg
    18601 gtacgaagcg aaacgatttg gatcgccgtt tcacccggga tacgcgctcc tggcgacatt
    18661 cggcctagtg gtcgcccacg cggcggtgaa catcttcaac gattacttcg actgccggag
    18721 cggtctcgac ttcaagaccc gccggacccc cttcagcggg ggaagcggcg ccatcccgca
    18781 ggggctgttg acaattcggg aagccctgtg gctcggcatc ggctgcctcg tcatcatcat
    18841 cccgatcggc ctcttcttca cgtgggcggc ggggtggaaa atggtgccgc ttctggttct
    18901 ggcgctctgc ctgatcgtcc tgtacacccc gctcatcctg aagatgggct accccgaatg
    18961 gtcccccggc ctcggactcg gcatcctgcc agtgatgggg gcctatttcg tccataccgg
    19021 cgattacacg gccagcgcct tcatcgcttc gatgccgtct ttcttcctgg ttcacaatct
    19081 gctcctgctc aacgaatttc cggacgttga agcggactgg accgtgcgga gaagaaccct
    19141 gccgatcgtt ctcggcaagc ggaacgccgc cgtctttttt tccgcggtcc tgctcttgac
    19201 ctacctctgg atcctctggg cagtgatgcg gcagttcatg cccgtctacg cgctgctcgc
    19261 gctgctgacg ctgcccctcg ccatccaggt gatccggggg acgttccggt atgacgagcg
    19321 ggaggtcttc atgacggtca tgacgaagaa tgtccaggtc gtgctggcga cccaggcgct
    19381 cttggccgcg ggatacatcc tgggagggat ctttccctgg tagcggcagg gatgacggac
    19441 ccggaccgac gcctggcgcc atcccccccc cacccgggga gtggacatcc acaaggagag
    19501 agaccccgat ggcagatccg cttttcacgc aagccgcacg gttgcaggcc atgctcgagt
    19561 cctgcgaact ctgtccgcac cgctgccgcg tggaccggac gagcggggag cggggcgtct
    19621 gcggactggg cgcttccgcc gtcgtggcca gcaccctggt tcatcacggg gaggagcctc
    19681 ccctttccgg cagccgaggc gccggcacga tttttctggc ctcatgcaac ctgggctgtc
    19741 cctactgcca gaactaccag atcagccggt cgacggcggg gacggtcacg gacgacgagg
    19801 gcctggcaga gatcgcgctg gcgctcgaga agcgggggtg ccacaacgtc gaggccgtca
    19861 cgccgacccc gcaggccccc cggatcgccg gagccctcct cgcggcccgg cgccggggat
    19921 tcgccctgcc gttcgtctac aactgcggcg gttacgaaaa ccccgaggtc atccggcttc
    19981 tggatcgctt cgtggagatc tacctgcccg acttcaagta cggcctcgcg gaaacggcgc
    20041 tcgggctggg caacgtgccg gactatgttc cccacgccct cgcttcgctc agggagatgg
    20101 tccaccaagt cggcgatgca ctggaacttc gggagagggt ggccacgcgg ggcatcatcg
    20161 tccgccacct ggtccttccg ggtcacgtgg ccaacagcct ggcggttctg aagctgatcc
    20221 gcgagaatgt ctcgacggcc gtccccttaa gcctgatgtc ccagtacacg ccggtcccgg
    20281 cggtgaagga ccatcccgac ctggggcggc gcatcacccg ggacgagtac gaccgggtcg
    20341 tggatgcggc cctggattgg ggcttctcca atcttttcat ccaagacgtg gacgaccggg
    20401 aactgacccc cgacttcgac cgggagtcgc ccttccgctg gtcctgagcg gacccggctt
    20461 ccgtccctac ccccagagga tcagacccgt ctcgtgcagg gaggacaggc gggggtgaaa
    20521 cggcaggacg cggacgccgt agcggtgggg gccgttctgc cggacccggt aatcgagggc
    20581 ataggtgaga acgccgtttt ccgcgcaccc gaccatcttc aggggcatgc agtccggccg
    20641 gccggcgaaa tccgccccgt ccttccgacc gatcatcagc tccacgagaa tctcctccgg
    20701 atccagtttg cccggatcga cgctggccgt cacgttgagc ggctgatcca ccatgacggc
    20761 gtcgccatgg atcccctcca cggtcacctc gagcaggcgc agggaagaaa atcgcatggg
    20821 gagcttccgt ttccagtccg ccagctcacg ggccaaccgg tactggtcgt cgacgagttc
    20881 acagctccgt tccgcggagg ggaggtacag atcgtggaag tattccagaa gcatccgctc
    20941 cgtgttgaag ctcggcgcca gggtccgcat ggagcgcttg atcatgccga cccatttctc
    21001 cggaacgccg gacacgtctc gatcgtagaa caggggcgcc acggtgtcct ccaggagttc
    21061 atagagggcc tgggcgtcgt ccaggtcggc attcggggtg tcttcggacc gttccttcac
    21121 aaccggaccg atcgtccagc cgttcgttcc gtcgaaccct tccggccacc acccgtcgga
    21181 tgtgctcagg ttgagcaggc cgttgaccac caccttctgg ccgctcgtcc cgctggcctc
    21241 atagggacgg cgtggcgtgt tcagccagac atcgacgccc tgaacgagac gccgggcgat
    21301 gcggatgtcg tagttctcga gcaggaagat cttcccccgg aaacgctcgt cccgcatgat
    21361 ttccacgacc tgttggatga gatccctgcc cctgctgtcg ttggggtggg ccttgccggc
    21421 aaagacgatg gccaccggcc gggtggggtg gttcagaatc cggtccaggc ggtcgagatc
    21481 cgacaggatg aggttcgccc gcttgtaggg cgcgaagcga cgtgagaagc cgatcaggag
    21541 tgccgacggg ttgatacggg cgtagagctc ctcccgccag gtcttggcat agccgtattt
    21601 tgccccatgc ctggccacgt catcccggag gaaattgatc agttgctgct tgagttcgta
    21661 acgggtgcgc cagaggaggg cgtctgggat gtcctggacc ctcgcccagc gctccggatc
    21721 cgtcaggtcc cgctcccagt cgaggccgag atacacgtct aggagttcct tcatgcgcgg
    21781 ggcgatgtag gacatcaggt gggcgccgtt cgtcacggat cggatgggaa ggtcggagct
    21841 gtggaacccc ttccagacgt cccgccacat ccgccgcgag acctcgccgt ggatccggct
    21901 gacggcgttg ctcaggtggg cggacttgag agcgagcacc gtcatgtaaa agggtttgtc
    21961 ctcgccgctt tccttgcggc caagttccat gaaccgggac caggaaatac cgctccactt
    22021 cacgaaactg ccgaagtagt gctcggtgag atcgtgatcg aagcgttcgt tgcccgcctc
    22081 cacgggggtg tgggtcgtga agacggtgct gccgcgcacc acctcggccg cctcgtcgaa
    22141 ggtcagaccc tcctcggtca taagcgccga gatgcgctcg aagatcagga aggcggaatg
    22201 cccttcgttg atatggtaga cgcggggggc gatccccaac ttcttcagga gcttcacgcc
    22261 gccgaccccg aggaggattt cctgttcgat gcgtgtccgc ggatccgaac agtacaggca
    22321 atcggtgatc ctccggtcct ggggcgtgtt gcggggcgta tcggtgctga gcaggtaaag
    22381 cgagacccgg ccgaccttga cttcccagat attggcatag aggacccggc cgggcagatc
    22441 aagagcgatc cggacctcga gcccccggtc atcctggaca atctggacgg gcatgttgga
    22501 gaagtcgttt tccggatagt cgacgatctg ggtgccgtcc ttgtcgatgc tctggtgaaa
    22561 gaagcccttc ttgtagagca ggccgacgcc gaccagcggg atgttcaggt cgctcgccgt
    22621 tttcaggtgg tcgccggaca gggtgcccag gcctccggag tagatcggca ggcattcgtg
    22681 gaggccgtat tccgtggaga agtaggcgac gggagaggcc cagcggatct ccgggcaagc
    22741 cgccagattc ttgtcgggga gccggtcgtc cagataaccg tcgaactgtt tgaggatctg
    22801 ggtgtaaaga ttcatatagc ttgcgttctc cgcggcctcg atcaggcgtt ccgtggagac
    22861 cgactccagc atgcggacgg gattgttgcc catcgtcccc cagattctcg gatccagcga
    22921 ggaaaacagc aagcgggcct gcgggtgcca ggtccaccag aggttatagg ccagctcgcg
    22981 caaccgctcg atcttgcggg gaaggttggc gacggcggtg aactgccgga agtggggctg
    23041 agtggaggcg gcgccggcga aggtgtgctt cgtttccaga cggtattccg tggaggtgag
    23101 cctgaccgcc ctttcgcggg caacattcag ggccttgccg taggccgcgc ggtagtgcgc
    23161 gtagaaatcg ctccagttgg cctgaccggc cacgtcatag gcctgccgcc gccgctcccg
    23221 ttcctcctcg tcggaccagg acaggcactg gcggaatacc tcgtagagct cgccttcgat
    23281 ggcgtcctgg gactgccccc ggcgtttcac taggagaacc cccttgtttt cacccatccg
    23341 gctctgcgcc cacaacccga atcctgcttg atccgtggtg accgtcggca cgctgtacgc
    23401 cgcgctctcc aggggcgtat acccccaggg ttcgtagtag gacgggaaga cccccaagtc
    23461 gcaccctgag agggcctcgt aatacgtcag gttgatcaga ccgtcgtggc cgttcaggta
    23521 agcggggacg aagatcacgt tgacccggtt ctgtgccgtg ttacgcagac cgacgcgatt
    23581 gcacgcctcc agaatcgggt cggaggcctc gttatggagg cggtaggttg cgagcggcgg
    23641 attggccgga tcgatccgga ccgcatcgcc ctgaagggcc gggatcaggt cggtatggcc
    23701 gccgatgacg aagagacacg ccaacacgga agtcccttcg cccatggctt tttccaggcg
    23761 tcccagggcg ccgagaaaga tgtcgatccc cttgttgcgg tactcgtaac gccctgagat
    23821 caccataatc ttcgtccggg cgggcatttc ccggccgaga aaccgggaag cggccgccag
    23881 gagcctctgg cgggccttca gggccgggcc gcggtccttc gtcagatcgg ggatgtttcc
    23941 catgtctagg ccgttgggag tgaccagatc cggagcgcgc cccaggaaat tcttggcctc
    24001 caaggcggtg atgtcgctga ccgtggtgaa acagtccgcc tccctggccg aggccacctc
    24061 catggaatac ttggcggcga tgttgcggtt cgtcgcttcg cgctgcgggg aaatgctgtc
    24121 catgtcggtg tagatgtcca tcccggaacc ggcgagggtt cgccccagca tggtggcgtg
    24181 ggtggtaaag accgtcccga tctccgggac ctgcttcttc aggcacaaga gcccggcgcc
    24241 gcacatccac tcgtggaact gggcgacggc cggcaggccg gccgtccgca acttaaggtt
    24301 gtagatcgtc tcgatcacct cgccgcaggc atagctgaac atgaccggtt ccacgtagtc
    24361 ccagccgccg gcgatggagt cgacgccata gtcctcccag atccggaaca ggagctggtc
    24421 cttgttgtag cgcttgctga aattgacaag gatgaccttg gggcggccgg gaatctgcca
    24481 gcggccgaag cggcagggaa tatccttgat ggcggttgct tcccggatcc ggttccagca
    24541 ctcctcgtcc gtttcttcga attccaggtt ggtcttcagg tccggtccga aaaggatgta
    24601 gttctgaccg aaggtgcgga tcgcttcatt gaccttgctc atgataaccg tatagatgcc
    24661 gccgaccttg ttgcagactt cccagctgac ctcgaacagg tacccttccc ggttcttcaa
    24721 catcgtggtt cctccaactc tggcctcgcg gcggtgaccc tttccgtaaa atcgtccagg
    24781 acgttcatga agttgacata ggcatcgtag ggtgatgcgt acggattgaa atagcggtgg
    24841 acgtccccgt cggcggacca tttggtgcac atgtagtaaa aatgatccga cgtctgcaga
    24901 agacgccagg tattcaagag atcggggtcc ttccgacgct gcacctgaac ggacagacga
    24961 tagagggcac ggagggcgtc ttgctgcata gcattgccct gccaggccgt caagtccctt
    25021 tcccgatccg cccaagtgac gatgccggga caatcgagga tgccttcgat cggatgggct
    25081 tccgccgcct cggccggagt ttggaaacga aacgccggat gcttgaggat ctctcccggc
    25141 aacgcctcga ggaagtggaa gatccccgtc tcggcccatt gatgctcccc gaaggtctcg
    25201 taatccatga acaggttgac aacccgggcg tcgtcggcca gggcatggat ccaatgggcg
    25261 aacttggccg cggtcagggg aaaacccgtc caaccccggt cggaaaaccg gaaggcgata
    25321 tcgtccgaga gacggtagtt ccggagcagg accctgaccc ggggatcgac cgccgtcccg
    25381 tagacccggt tcggggaacg cccccgcaga agcggctccg tcccctcggc aagcatgacc
    25441 cggtagccca gttccgcgac gcgctcgacc agggcgttgc tgaagatcag ctccgtaccc
    25501 cggaaggtga ccgcctcccg accgaacgcg tcgcggatcc gctcccggtg acgtcgtacc
    25561 tgggtgcgga attcctccgg atcacgcgga aaggccaggg tgtgttcata ggtttcgttc
    25621 agaaattcga cgcagcccga cgcggccagc gcgcggaagg cgtccaggac gtccggatgg
    25681 tcgatcgcga gccggtccag agtcaccccg gtgatggaaa aggcacagcg gaagcgcccg
    25741 gcgtgggtcg cgacgaggtt gccgaggagc cgggtcgcgg gccggtaaca cttttcgacc
    25801 acccgggtca ggatgcggcg gttttcctcc tcggcttcgt agcgcgcgaa ggccggggcg
    25861 ccggcggcat cgatgtcgaa gacgctgaaa cgcttcagac ggcggggctg gtggatttga
    25921 aagtagagac agaccgaggg catggcgttt atccgttcac ggaccgagga gcccgcgata
    25981 aacgtcgagg acgcggagcg ccgcagtttc ccagcggaga gcctccagtt ccccccgcga
    26041 gcgggccacg atcgcctcgg ccagctccgg ctgtgtcagg atagcgacga tggtccaggc
    26101 cagggcgtcc acgtcccaga aatccacctt cgggacgtgc cgcagcacct ccccgacgcc
    26161 cgactgcttc gagatgatga ccgggacgtc atgcagcatc gcctcgagcg gggtgatgcc
    26221 gaagggttcg gaaacgctgg gcatgatgta gaggtcgctc agggcgaaca tgcgttcgac
    26281 ctcctccccg tcaagaaagc ccgtgaagtg gatctggcgc ccgaggccca gttcggccac
    26341 ccgttcaatc gtctgcggca gcagatcgcc cgcaccgacc atgacgaagc ggacgtccgg
    26401 cagaacgctc aggaccttgg ccgccgccgc cacgaaatgg tccgggcctt tctggaaggt
    26461 gatgcggccc atgaaaagga cccatttctt cccgggaggc ttgaacgcac ggtaggctgg
    26521 cgccgcctcc cgacgggaga cggcgttatg cacgacagag accctgccgg cggggatgcc
    26581 gtagtgacgg acgatcaggc cacgggtata gtgactgacg gcgatgaccc gatcggccaa
    26641 gctcatgccg aggcgctcga cggcaaagat cgccggatgg acgttgttcc cgctccgatc
    26701 gatttccgtg gcatggacgt ggtacacgaa gggccggccg ctgacccggc gggcctcgag
    26761 cgcggcggga acggtcatcc agtcatgggc gtggatgacg tcgaaggacg cctccgcggc
    26821 aatccggccg gccgcctttc cgtaccgcag aacctcggcc atcagatcgg atccgtaatt
    26881 tccggaaggc gcgagcatcc cggccgcccc gcccgaaatg ccgggatacc ggtcttcacg
    26941 caggtaggga tggaggggag agtcgatgat ccgaagcagg acatccaggg gccttcctgg
    27001 cataccggcc ggtggcgccg cgccctcggc gtcccacccc ggacggtcgc tcgccggcac
    27061 ttcggccgcg gacacgagcc ggacatggcc gggctcctcc cccgtccggg cgcacgggat
    27121 gacataggtg atcgtctgtc ccagttcgct catggcgcgc gtcagcccca ggcaggccgt
    27181 ccccaaaccg ccgctgattc gggggggaaa ctcccagccg aacatcagga tgcgcatgcg
    27241 tcctccgctt tctcgccctc gcggaacccg tcgacatacc ggagtgcttc gaaactattg
    27301 caggatgtgt cccttctgtc aatagggaaa gggtctgtct gccgccgccc gcgtttccga
    27361 cagggccgtc cgggaaaaag gttttacaag gtccccggat tcgcatatga ttccagccgg
    27421 acgtgtctcc acaccgggga cggggacagg gatgccatac ggaagggaaa cgccatgaaa
    27481 ctgtttgcca tcagcgacat tcacggggcg acgaagccca tcgaagcggc agcggcgctt
    27541 atccgcgcgg ccgacggggt cgttgtctcc ggggacatca cccgcggcaa aacgcggtcg
    27601 gaagcggagg aggtcgtcgc ttgcattgag cagtattcca cccgcatcct cgccgttcac
    27661 ggcaactggg accggccgga ggtgatgacc ttcctcgagg acaaaggcta cagcctgcac
    27721 gggacgggac ggacgcttga cggaaccggc tttttcggtc tgggcgggtc atccccgacg
    27781 ccgattcaca cggcgacgga atacacggaa aaagacatcg acggtttgct ccgagtcgga
    27841 taccagacgg tccatgaagc ggcccgcacg gttctggttt cccatgttcc tccccggggc
    27901 gtcagggacc ggtcgttcct cggcctccgg ggcggcagcc acagcctgaa gcgcttcctg
    27961 gaaacgcacc cggtctccct gtgcctgtgc gggcacatcc acgaggccgc cgggatcgaa
    28021 cgcttccggg acacgctcgt ggccaacgcg ggcagcttca agcgggggca ttacctcacc
    28081 gtcgagcttg gccccacgat cacggtgacc gccggacggg ttgcccactg atcccggtgc
    28141 ggtggggtag cggatccacc gcaaccgggc aaggaatcct ctcctgtcga acgaggcttc
    28201 agcggcagcc gcgccgaccc gcggcgtcct ccagttcggc cagggcctga agccgggaag
    28261 cgatccggat gaaggcatcg gcggcccgat cgatctgctc cgccgtattc gtgcggccga
    28321 ggctgaaccg gacggtgccc agggcctccc ccacggagag attcagggcc gtcagaacgt
    28381 gggagagggt cggcgactcc gacgtgcacg ccgaaccggc cgacacggcg aacccctgct
    28441 tgtccatctc ccgcaccagg acctcccccg tcacgccgtc gacggaaacg ctcaggatgt
    28501 tgggcaggcg caactccggg tgcccgttga agcgcagttc cggaagggcc cgccggatcg
    28561 ccccctccaa tcgatcccgc aaggacgcaa gcaggacgcc ctgttcggcg aggtcccgcc
    28621 gggcgatctc gcaggccctg cccattccca cgatgccggg aacgttttcc gtaccgctcc
    28681 ggagcccccc ctcctggtgg ccgccgaact gccggggtgc caggcgcgtc ccccggcgga
    28741 catacagggc gcccacgccc ttcggcccgt aaatcttgtg ggaggccagc gacagaaggt
    28801 ccacgccaag cgcctcgacc gccacgggga tcttccccgc ggtctggacc gcatcggtgt
    28861 ggaagggaac gcccctctcc cgcgcaacgc gcgccgcctc ggcgatgggt tcgatcgcgc
    28921 cgatttcgtt gttgccatgc atgatgctga cgagaaaggt ctcgtcgcgg atggcctccc
    28981 gcacggcctc gggatcgatc cgtcccaggg gatcgaccgc caggacagtc agtttgcacc
    29041 ccgcttccac cagggatttg cacgcattca gcacggcggg gtgttcgatg gcggtggtga
    29101 cgaggtgatt ccgggcgggc gggcgggcgg cggcggcgcc gaggacggcc aggttgtccg
    29161 cctccgtccc gccgccggtg aagacgatct cttccggcgc cgctccgatc agctccgcca
    29221 cctgagaccg cgcggtttcc aaggcctccc gtgcgtcgcg cccgcaggcg tggatgctcg
    29281 aggggttgcc gaaacggccg ccgaagtacc gcatcatggc ggccattacc tccggatccg
    29341 tcggcgtcgt ggcggcgtgg tccaggtaaa cggtgtccat ggttccgatc tctctccttc
    29401 cggacggcag gattcgccgt ccataccccc ctgaaccggc gtttgtcaac tccaatcagg
    29461 gggagtggaa aaagtcctca cccgaacacc ggccgcctcc cgggaaaatc acccgaaagg
    29521 cggccagccc ccgccacccg tcgatcaaac cggggcaaaa tgctcgaggt cgagtatgtg
    29581 gccgatccgt tcctcctgcc aaacggcctt gagctgcatc ccgttgtgga cgatcccgcg
    29641 caccttgtcc agatccgcca agtcgattcc gcccaacaga tggagaaaat ccacgtccgt
    29701 tccatccagc cggatcaagg cgccgatgaa cgggggttcc gtcggcatct cgaaataccg
    29761 gtaattgatg tagctccagg cggtcagctt ccccgtctgg gccacctcca cccaagcgtt
    29821 tgtatccacg aaacagcgcg ggcagaaact ccgggccggc accaggatcc gaccgcattt
    29881 ctcgcacttc gtcccgtaaa tcttcttttc cctcatgccc tcgaaaaacc gcgtccatac
    29941 cggtccgtag gacagttcgt aaggaagccg aatctccgtc ttcagcgtct tgtattcctt
    30001 ggccatctca atctcctccc tcgatctttt gaacgctcac agttccgaac ccacgatcat
    30061 cacgccattg aattggtcga caccccccat cgcgtgactc aaggccagtt ttgcaccggc
    30121 aatctggtga tcccccgcgc gtcccatgac ctggagggcc gcctccgcca cccggaccag
    30181 cccggtcgcc ccgatcgggt tcgtgcacag cgtccccccg gaggggtcgc acggcagctc
    30241 tcccgtccga tcgaacaccc ctttgagcac caggtccggg gactgcccca ggtcgcagaa
    30301 gccgaaacat tcgtagaaga gcatctcctg gaaggtgaag gggttgtaca cctccgccac
    30361 gtccagttcc ttccggggat ttttgatccc cgcctgatcg tacgcctgct ttgcggcgcc
    30421 gaccgcgctc tgccagacgc ccttgtccga atcgccgaag aagtactcct cgccccgata
    30481 gcccacgccc ttcacccatg cgacgggccg gccgagctgc ttcgccttct cttcggacat
    30541 gaagatcacc gcgcaggccc cgtccgaggt cggacagacg tcaaggagtc gtaccgggta
    30601 ggtgatgagc cgcgcgttct taacgtcgtc catcgtcaca tggatcttca tgtgggcata
    30661 gggattcgcg aacgccgcct cgtgatgctc caccgagatg tgcgccgccg cctcgcgaat
    30721 cttctcctgc ggaatgccgt accgctccgc ccacatctga gactgcatcg aaaagacgcc
    30781 cggcgccccc gcgatgaaaa agcgctgata aaacggttcg accaccgtcg tcatggtcgc
    30841 ctgcgcgtcc ccctcgttca tcttctcgtg gccgaccgcc agcacgcaat ccgccattcc
    30901 ggacgatatc caataatacg ccgtatgggc aatcgaaacg cccgtcgatc cacatgtctc
    30961 cgtcttcagg atcggcttgc cgaccgcctg catcgcatcc gcaaagtaaa aatgcgtcag
    31021 cgcgatcccc tccatcatcg acggcatcgt ccccgagacc accccgtcga tgtcccgcgg
    31081 actcaacccc gtgtcctcga agacccgaac caccgcctcg cggaccagat cgggatagct
    31141 gatctccatc cgcttgccgt ggtgcgtctg tccaacccct acgattccga ccgctcttcc
    31201 catcgctctt ccctcctaac gtccgatgat cgcaacggca tgacactgac ccgcgaagcc
    31261 gtgcgtccca tgcgccaacg ccgttttcac cgacttcgga acctgatgct cgcccgcttc
    31321 gccgcggagc tgcaacgtcg cctcgatcag ccgctgaagc ccccgggaga catacgggtt
    31381 gttcgccaga acgcctccgg acggattgat cggcagggac ccgccgatct gcgttgcgcc
    31441 gctttccagg aacttgccgc cctcaccggc gccgcagaaa cccagatctt cgctccacaa
    31501 cagttcctga aacgcatagg gctcgcaaat ctcggccacg tcaatgcctt ccttcggatt
    31561 cttgatcccc gccatgtcgt aggcacgttt cgccgccctc gtcagttccc cgcgcagcag
    31621 atcccggtcc cccagggtat acgtatcaag ggaacttccg tagcccttga accagaccgg
    31681 cttgtccgtc agtttcctgg ccttcgcttc gcccgccagg agcatcgcga gcatcccctc
    31741 ggacttcggc gccgcctgca gctctgtcag cggatccgcc aaggtggggg atcgcaggat
    31801 gtccgacacc gtgtatttgc ccttccggtg cgcgtagggg tttttcaggg cattgcccag
    31861 gtttttcgcc gccacggcgg cgcactgttc ccgggtcacc ccgtagcgct ccatgtaaag
    31921 gttcatctga agcgccgccg ccaccgtctc cgtcaatccc acgtcgcgct ggtaaaaggg
    31981 gtccgcgaac atcagcgtgc aggcatcgtt cggcggattc tcgcttccct tgcacagact
    32041 gatcaccagc gccgtttcat attggcccgt catgatccgc atcaccgcgt acatgaaggc
    32101 aaataacgat tctccgtcct gacgtgacgc gttcttcaaa aacgcccccg tcgtatccca
    32161 gtaatacgcg ttcgcacagg aaatcccccc gtgaaagatg tcggaggagg ccgacaccac
    32221 cgtgtcgacg tcctccctgc ccaggcccgc cttgtccagg cattcccggg ccgcccggta
    32281 ggcctggtcg tagaaattat ccttcgactc ggctccgggg ctttgcgcca ccgatacgat
    32341 tgcgaccctg tcccccataa cctcactcct ttcgttaaga ttgacgattt cgtgaaaaag
    32401 ttgcggcgtg ttaaagacgg cgatccaagg ctattgtgaa acggtcagtg acaacgacat
    32461 aagccgggcg aaagcccgat agggtatggt ttcgacggta tacacggccg gcaggaagtc
    32521 aggccgcacg atgaaacacc gagtggcgct ctttatagca caaaatgggc ggggatttca
    32581 aaaaaatttc ccccttcccg cctttcacgc ttcctgaatc gtaatgccca gtttcagaaa
    32641 cgcctccgag gcgcttgacc atcccgcgta tggcacccac cggtcgagct cgccgttcag
    32701 gcaccccgtc cggaagtgca cctcttcgtg gggctgcgtt ccgccgatca gcacccgttt
    32761 ttcttcgagg ccgcccagca gacggaaggg atccatgacc atcagggcga tgtcgtcgtg
    32821 ctcgaaccgg ccggcatgtt ttcccaagag gcggtgcatc tgttcgccga ggccgtcgat
    32881 tggaaacgcg gcgaacgcgt ccgacaggcg ggagaggaag gagcggttga tgcagaagcg
    32941 aacgaggtcg tcgaaaccgt cactgtagag ggccacgcgg gcattccggt ccgtcacgcg
    33001 gcactcgatc acgtcaacga tttcccggct ccgccccgcg aagaccatgt tgcgcccggt
    33061 ctcgaagagg acgtcgccgc tgatggaatc gatcaccagc accccgctgt ctccgccgtg
    33121 tgccaccgtc aggacgactt cgtgatcgtc gccactgacg gccacacagc tgaaggtcgt
    33181 tttgtgctga tacttctgcc cggcatacac ccgccggttg atcaattcct tccagtccgc
    33241 cgccgatgcg ggaacgccgg cctgcatcag ggcatcggac agccggcaga ggatgtcccg
    33301 cgaggcccac gggaaccgct ccgtcccgtc cgccacggca aacacccggt ggcggaaatc
    33361 gaaatagaga cagtcgccgt tcccgtaccc gctgcgaacc ttgtccgtac cggtaaagat
    33421 gctcccgctc agcgcgtcct tgagcctcaa cgtccgcttc gcggcattga tctcgatgac
    33481 gtcagacggc gccatacatc aatcgatgat ccggaaatac tcgatgtccc gggtgttccc
    33541 tttgcgctct tgggcgtaaa caggctccac ccgggcgccg atcttgacct tgctctcatc
    33601 cttgatgtcg atatagtgct gcagcagggt gtcggccccg tcaagcttga tgaacccgta
    33661 cccgtaggga accggcttct gctggcccgt ctccgggtcg atgaaggcaa agcgcaggat
    33721 ggtgaacgtg ccgatcacgc ccttcggacc cacctcgacc cagtcgtcca tctcgacgaa
    33781 acacttcccg cagacgcccc gcggcgggac gtagaccttg ccgcacttgg ggcacttcag
    33841 acccatcagg cgcatgttgt cgcgggtttc cgtgaaaaac ttgctgccgt gcattcccac
    33901 cgcatatttg aacggctgcg ccgcatcgcc ggattcgata atcaactgtt ccttttcagc
    33961 catggtctca cctctcaatt cggttttctc ttgccgtgaa ggagcatgtt ggtccacatg
    34021 cagcccccga aacccgtgct gacggcaaac ttgacgtccg gaacctgccg cccttccgcc
    34081 ttgcccatga cctgcatcgc cgcctccgcg cagcggatga ggccggtcgc gccgatgggg
    34141 ttgcaggcga tcacgccccc ggaggggttg accggcagct ccccgcccat gtcggtgttg
    34201 ccgtcccaca cgaactgcgg cgcctcgccg tggccgacga tccccatgtc ctcgatccag
    34261 atgagaccgg cgaaggaata gggctgatac atctcgatga cgtccagttc tttccggggt
    34321 tccttgatcc ccgccttttt gaagacctcc ttcgcgcact gctccatgct ggtgagccgg
    34381 ccccagtcgc cgtcccccag gtaggaatac gtgttgtgcg cggaggttcc ccagatccag
    34441 tccggaaccg gacagatctt ctcggcgctg tcctcgctcg ccatgatcac cgcgcaggcg
    34501 ccgtcgctcc gggggcagac atcgagcagg tgaatgggat ccgccagcat cggagaggcc
    34561 agcacgtctt ccagcgacac ctctttccgg agctgggcat gcgggttgtt catcgcgtgc
    34621 tttcggtccc gcaccgaaac ccgggcgccg tcccggtcgg acgcgccgaa gcgcgccata
    34681 taggcctgcg cctcggcggc cagaccggag atggcgccgg caaacacctg ccggtcccag
    34741 atggggtcga aggccgtcgt gatggcgccg gtggtatccg attcggagtt cttctcccag
    34801 ccgatgatca ggaccttctc gaacataccg ctgccgacca tgtgatagcc gccgatggcc
    34861 agggaggccc ccgtcgtccc gccggtggtg atcttgatca ccggcttcat caccccgccg
    34921 cagccgtcga cgctccagca gtccacatag ttgatcccct cgaaatgatc catgttcccg
    34981 atgaggatgg cgtcaatatc cttcatcgtc aggtcggcgt cggccagggc gcgctgaacc
    35041 gcctcgttga tgagttcctg accgttcaca tccggacgat ggctcttgtg gaaggtctgc
    35101 cccgttccta cgattgctac tcgcttcgcc atagttcttc ctcctacttt tccttgtcca
    35161 gaatccagac gcagtgcgac tgaccgcaca gaccgttcac gccgtgggcc agacccgtct
    35221 tggccttctt cacctggtgc gccccggcgt cgccccgcag ttgcaggacg atctcggcaa
    35281 tccggtaaag tcccgcggca atcacgggat gacccgacag cagccccccc gacggattca
    35341 ccggcaacgc gccgtccagg ttgaattctc ccgccttgag acgtgcggcc gccttgccgt
    35401 agtccgcaag ccccatctct tcgagccaga gcagttcctg gtagctgaag gcatcgtaga
    35461 gctcgacgag atcgatctcc ttccgcgggt ccttgattcc ggccatggcg tacgccttct
    35521 tcgcggcctc ggtcagggcc ttgcttcggg ccagatcgcg gtcgccgaag aagaagctgt
    35581 ccgtacaaaa ggcgagtccc cggatccaga tgggcttctg tttgaacttc gtggctaccg
    35641 attcgtgcgc gatgatcacc gcgcagcacc cgtccgacac cggcgaacag tcgagcttgc
    35701 gcagtgggtc cgcgatcatc tcggaggcca tcacatcgtc aacggtgatg ttcatcggga
    35761 gctgcgccat cggattcttc agggcgttgc cgtggttctt caccgaaacc agggccgcat
    35821 cctccggctt cacacccgta cggttcatat aggcgttcgc ctgcatcgcg ctggccgtga
    35881 tcatgtccat gcccagcacc cgctcgtaga tggggtcgaa ggccgcattc gtgatcaggc
    35941 tggagacgca ctgcgaaccc ttggagtgtc ccgtgaccag ggtggtcttg taggaacccg
    36001 acagcgcccg cgcgttggcg taaaaagccc cgtacgtccc gtcgccctcc acgcaggaga
    36061 cgttcttttc cgccgcgccg ctcacatccc ccaccgccat cagggaaatc gtccgtccat
    36121 cccagaaatc gctggacgtg gtgacgacgt tgtcgatgtc ggcgatggtc attcccgcat
    36181 cgctcaacgc cttgtttacg gcctcccagg tcatgtcggc aaaggtctcc cgccgctttt
    36241 ccgcttcgat ggtcgtcatt ccgacgccga tgattgctgt tctgtccatg atccctccct
    36301 acttgacagg cttaaaatga gaaatatcgc ggatgccgcc cgtccgtgac tcagcaaaaa
    36361 ccgctttcac acgcataccg atcgtcaccg acccgggatc gacttcatcc aggtagtgaa
    36421 gcaaggcggt gtcagcccca tccagtttga tcagccccca gatcacgggg accttcttgt
    36481 cgcccgggat cgcctccagc tgccgccggg acacgctgta ggaaaccacc gtcccctcgt
    36541 ccgagacatc cacccagtcc gtgttctccg tgaaacaggt cgggcaaacc ttgcgcggcg
    36601 gcataaacac cttcccgcac ttgccgcacc gtggagcgat gatctttcgc gcgtcccgca
    36661 aactgatgaa gaacttgctg gccgtatccc ccgcccacca gctgtaaggt acgctgatct
    36721 tgctgttgtg aaccatcggt tcaattccct tgaagatctc tgacattcct cctgcctcct
    36781 ttcgtccaaa tggctttcac gcccttcatc tccggacggc cccgctcctg cggcgccaac
    36841 cgttttaacc atgccgtgca aactccctgt ttcccgaact caccgcggaa caaaccatat
    36901 attgattgac aagtcaatca aaaaacctta tgagggtccc accgacaaca aggaggcaaa
    36961 aggcatgtca caggttaatc tggcgcagat ttttcgtcac caggcacaga aatacggtga
    37021 gcgtctggcg gtcgaaaaac ggctgcgtaa tcagtggcag ggctggactt ggaaaacgta
    37081 ctacgacacg gcccgggcgt tgggactcgg cttgtaccgc ctcggcgtgc gccgggggga
    37141 ccgggtcggc ctgctcagcg aaaaccgcct ggagtggatc ggagcggaca tgggcatcat
    37201 ctgcatcggg gccatcacga tcccgatcta cgtcaccctg ccggccgcgg aggtggctta
    37261 catcctcggc aattccgacg ccaaggtctt catcgcggaa aaccaggccg ccgccgacaa
    37321 ggcgctgaag atgaacgccg acctgccgga gctccagaag atcgtcgtga tcgaagccga
    37381 cggttgcgac ctgtcccatc cgctcctgat gagcttttcg gcgctgctgg acctcggccg
    37441 cgggctcgcc gccgaggagc cggacctgtt tgaaaccctg ggagacgcca ttcgcacgga
    37501 ggacatggcc accttcgtct acacctccgg aacgacggga ccgccgaaag gggccatgat
    37561 cagccacagg aatatcctgg cgatcttcga cgccctcgat acggtcgttc ccgcctacga
    37621 aacggacgaa accgtcccgt tcctgccgct ctgccacgtt ttcgagcgga tcgccggcca
    37681 tttctacggg atgaaggtcg gcatcacggc ccactacgcc cagagtttcg acaccatcgt
    37741 cgaggacatc aaaaccaagc ggcccaccat cgttctggcc gtcccaaggg tctgcgagaa
    37801 aatctacgcc cgcatccagt ccgaggtgaa gagccagccc gcctggaaac aggggatctt
    37861 ccgctgggcg cagcgggtcg ggtctgagct cagcctgctc aaggagcaga aaaagcccgt
    37921 gccgcccctc ctcgatctcc gctaccgact ggcttacaaa atggtctatc agaaattgaa
    37981 cgaaggtctg ggcggccggg tccgctggat gaccgccgcc ggcgcccccc tgtcccggga
    38041 gatcgccgac ttcttcgtgt cctccggtgt ttttgtcatc gagggatacg gcatgacgga
    38101 aacgacggca cccgtgagcc tgaacgtgct gggggattac cggttcggca cgactggccg
    38161 gcccctgccc tgcaaccggg tgaagatcgc tccggatggc gagatcctcg tgaaggggga
    38221 caacatcatc agcggttact ggaaaatgcc cgagcagacg gcggcggcct tcaccccgga
    38281 cgggtggctc atgacgggcg acatcggaca cctggacggg gacgggtttc tcgtcatcac
    38341 ggaccggaag aaggacctca tcatcaccgc cggaggtaag aacatcgccc cccagaacat
    38401 cgaaggcctg ttcatgaagg accccctgtt cgagaatttc gtcgtcatcg gcgaccggag
    38461 aaagtacctg accgccctgg tcaccctcaa cctggaagaa gcggcccgcc tggcccgctc
    38521 ggccggattg gccttcgtcg acgcgcagga ccttccggag cgggaggatt tccggagcat
    38581 cgtcgagaag catgtcgccg agcggaatca gcacctggcg aaatacgaga ccatcaagta
    38641 cttccggatc gttccccacg cctttaccga acagacgggg gagctgaccc cttccctcaa
    38701 ggtgaagcgc aaggtggtgc tggaaaagta tcaggaactc atcgaaggga tgtaccccgc
    38761 ggacgtggtc tgatgggggc acgtccccgc cgcctcccga accgttcatc gcattccgcg
    38821 cgcggggatc gcaatggttg cagatcggct caggcgaact tccaggtcgt agcggcggga
    38881 ccgtcctgga ggatgacgcc gtgctcggcg agccgctgac ggatggcgtc ggcctgggcc
    38941 cagtttcggg cctgtctcgc tgtcaggcgc tgcgcgatga gatcttcgat ttcggccacc
    39001 tccagtccac ggcggccggt ttcccgccgc tgatccatcg cgaaatagtc gcggggatcc
    39061 tggcggaaca gaccgaggac gtctccgcac cggtcgaagg cctgccgggc cgccgccaga
    39121 acgagggtcc tctccgggct cgccgcctgg ccctcggcgt ccagataagc gttcacctgc
    39181 cgaactccgt cgaagatgta gccgatcgcc cgggcggtgt tgagatcgtc gtccatggcg
    39241 cccgcgaagc gctcctgaag ccccgtcgcc ttttcgtaaa gccgccgatg cccccccgtg
    39301 aggcgctccc ccgcggttcc gtcggccgcc ggccccccgg acagcgccgc ctggatcttc
    39361 cggcgggtct catagaagcg gtccagaccg gttcgggtct cgcggagggc ctggtcggag
    39421 aaatcgacgg gcgaccggta gtggctctgc aggatgaaca gccggagcac ctccggcggg
    39481 tggacggcca gcatctcgcg gatggtcgaa aagttcccca gggacttcga catcttttcc
    39541 ttgttcacgc gcacgaagcc gttgtgcatc cagacgcggg ccaggggccg gcccgtggcc
    39601 ccttccgact gggcgatctc gttttcatga tgggggaaaa tgaggtcctc cccgccgccg
    39661 tggatgtcga acgtgtctcc cagataacgc tggctcatga cggaacactc gatatgccag
    39721 cccggccgcc ctttccccca agggctgtcc cacccaggct ctccgggctt gctccccttc
    39781 cagagggcga aatccatggg gttcgttttc ttctcgttga cgtcgatccg cgccccggcc
    39841 aacatctcct cgaggccgcg gccggagagt tttccgtacc cggcaaaccg ctcgacggca
    39901 tagtagacgt ccccggaaac gacgtaggcc aggcccttct ccaggagcgt ccggatgagg
    39961 gcgatcatgc cgtcgatatg ctccgtcgcg cggggcgtga ccgtcgcgcg ctcgacgccc
    40021 agggcggcca tgtcctcgtc gtgcgcggcg atgaacgtct ccgccacctc gccgatggtc
    40081 cggccctcgc gttcggcgcg ggcaatgatt ttgtcgtcca cgtcggtgaa gttcttcacg
    40141 taggtcacgt catagccccg gtatctcagg aagcgggcca ggacgtcgaa gacgatggcc
    40201 gcgcgggcgt gaccgatatg gcagagatcg tacgccgtga tgccgcagac gtagatcccg
    40261 gccttcccca cctgaagggg ggaaaagtcc tctttccttc gtgtgagcgt gttgtagaaa
    40321 cgcaccgaca tcatgggttt cctttccgga aacggcttcg atcccggctg gcccgcaccg
    40381 gccggagtca ggggaacagg gggagcatct ccagcccggc ggcctcctcg aaaccgcaca
    40441 tgaggttcat gctctgaacg gcctgaccgg cggcgccctt caccaggttg tcgatgacgg
    40501 acacgacgat gacccggccc gttctttcgt cgacggtcac gccgatgtcg cagtaattgg
    40561 agccccgaac cgccgcgaga ttaggaaagt tccccgaccg gcagatccgg acgaaccgct
    40621 cccgttcgta aaaggcctcg tagagcgcct tcacgtcggc cgtggacgcg tcccggttca
    40681 gtgtcccgta cagggtgctc aagatccccc ggttgagcgg aagcagatgc ggcgtgaagg
    40741 aaatccggat ggcctccccg gccagggcgg tgagctcctg ctcgatctcc ggcgtatggc
    40801 gatgctgtcc gccgactttg taggccttga accccccgtc cacctcgcag aacagggagg
    40861 tcaccaccgg ttcccgtccc gccccgctga cgcccgactt cgcatcggcg atgatcgtat
    40921 ccgtcttgag ccatcccgcc ttgagcaggg gggcgagccc cagaatgatg ctggtgggat
    40981 agcagcccgg gttggccgtc aaaaccgtcc gccggatggc atcccggtac agttcgggca
    41041 ggccataaac ggcggcgggc agcgtgtccg ccgccaggtg ccgtgcatac cactgctcgt
    41101 agacggacgc ctcccgcagg cggaaatcgg cgctcaggtc gatgaccttg cagccggccg
    41161 cgagaaaccc cggggcgacc gacatggata cgccatgcgg aagggcgaga aacaccacct
    41221 ccgcgagttc ggcgaacgcc gccggcgtgg catccatgaa caccaggtcg gtcaaatccg
    41281 caaagacggg atagagctcg gcaaccgcct tcccccgata ccggcgggac gtgaccgccg
    41341 cgacggtgac ccgggggtgc cgcagaagta accggagcag ctcctgtccc gtatacccgc
    41401 tggccccata aatcccgacc ttcatcatcc ttcgactcca aaaaaaaggg ggccgaggcc
    41461 cccatggttt ctaacgcttg gagaattgga acctcttgcg cgcgccgggc tggccgtact
    41521 tctttctttc cttcatccgc gaatcacggg tcaggaaacc cgccttcttg agcagggaac
    41581 gcatctccgg atcgtactcg atcagggcct tggcaatccc gtgcttgacg gcgcccgcct
    41641 ggccggcaac cccgcccccg cagacattca cgaagaagtc cagtttgccc ttctggcccg
    41701 tcatctcgag cggctgcatg atcagcatct tgaggctgtc ccgcgtgaag tagtcgtcga
    41761 aattccgctt gttcaccgtc aggacgccgc tgccttcctt catatacacc cgtgccacgg
    41821 atgatttcct tctgcccgtt gcatagaacc gtttgtccgt catcgccttc tccaacccgt
    41881 tctctcctgt tgcgggcagg acgcccgcac cgtcgccccc gcctacagaa ccaccgtctg
    41941 cggacactgg gcctgatggg gatggtcatt ccccttgtat atcttgagct tccgcagcat
    42001 ctgccgcccc agggagttct tcggcaacat cccgcgcacg gcggtgtgga gcaccttctc
    42061 gggcgccgtt tccagcaatt tcccggccgt cacctgcttg atcccccccg gatagccgga
    42121 atggcgcgaa tagaccttgt cggtcagttt cctgcccgtc aggaggatct tttcgcaatt
    42181 cacgacaatg atgaaatcgc ccgtatccgt gtgcggggta taaacgggct tgtgcttacc
    42241 ccgcagtcgc cgcgcaacct cgctcgccag ccggcccagc acctgccctt cggcatccac
    42301 cagataccac gcctccgcgc gttcttcctt cttcgcctga taagtcttca taaaaccacc
    42361 gaacctcgag aattagaaag gggacgctac gaaatttaac gcggtttgtc aagggaaaac
    42421 ctgcatgcaa atatgcgtgc cggcaagggg tcaatggacg ccggcctgtc ccaggccgta
    42481 aagggagagc agcagcgtga cccgctggtc gtcctccttc ttctcgtacc cgaggccgat
    42541 gctccagcat tgacggtggt aggtcacttg cacgtaattc tccaccttca cgttgtcccg
    42601 gaggttgttc cggagcgtgt agtccaagcg gatcgtccgc gtcagatcgg ccttcacggc
    42661 cagattgatc tcttcgagaa tatcccgcgt attgtgatag ccgagcccca agttgacccg
    42721 gccgacccgc gcgttcaggt cgtagttggt tcgggtccag gcgtgttcat gcacgtcata
    42781 aaggttgcgg acgaccaggc tgatctccgg aatcggtgcc atgacgaggt caagggccag
    42841 cacgccaaag ggtcgccggc cggggtcgcc gggaccgtcc cgatcccggc gggcttcacg
    42901 gatgtcgaac tgctgggcca atttcagccg caagagttcc cgataggcga tctggtcgtc
    42961 gttcccccgc accttggccg tgagcacatt ggtgatgccg taggaaacga gattctgggc
    43021 attcacgcgg gccacgtaat cgggaagcgc cacctggcgt tccagcgtgg catccgcata
    43081 ggcataggcg atctccggct tgatcgtgtg ctggagccgc tccaaggact ttccgggacg
    43141 atcgaagacc cgggaaagct ccgtattcag agaaaagccg gcgtgaaaaa cctcccggtc
    43201 gccgtgccgg tctccccgtg ccaccccgtc gtcgtcccga tcccacaggg tggcccgcac
    43261 accgacctcc ggcgtgaact gggcatatcg gcccagcttt atcggcagcg tgaagacggg
    43321 cgcaacgtcg tacaaatgcc cccgctgccc ctccccccgg tacagataat cgtacgtggc
    43381 cttgaggccg tagtagaccg gctgaccgaa aaggggaagc gtcgcccggt tcaggatgat
    43441 ctcggggtac cgctggaggg tgacgtcgtt ggagggaacc gtgaaatcat cggtgtaacg
    43501 cgccagcgcc gtcagattgt agcgggacca atccttgacc agccgcacgg tcgaatcgag
    43561 ggagcggagc gacgcgtcac cgtcgaagga aaccttctcg aagcgttccc gccccccccg
    43621 gtcgcgatgg tcccggtagt agttgcggtc atcgaaatcc ttgaaatacc aggcgtcaga
    43681 cacccgggcg atatccgccc tcagataaaa tccctcggta aaaacggtct cgtggttgag
    43741 gaagagcgac cagcgcaacc ggtcggaccg ccagtcccgg ctgacggggc ccgatgtctc
    43801 cgtcacgcgc ttccgatcgt tgagaagatc cccgtagagg acgccggacg aacgctcccc
    43861 ggcgaaatac cggaattcgg tcccctcctt gaagccccgt ttgtccatgt agcgcgaata
    43921 gaaggtggcg tcgagcgacc gggagatggc ccagtaaaag gggacttcgg catcccagcc
    43981 gtgatcgtcg gagtgggcca gatagggcag gagaaacccc gactggcggg tcgtcttcgc
    44041 cggaaagatg aaatacgggg catagaggac cggaatgttc cggatcagga gtttcccgtg
    44101 tttcatggtc ccgtaaccgt cgaccgtgac gtttatttcc cggctggtca gccgccagtc
    44161 cggccggggc ccgtcgcagg tcgtcgcctc ggccccctcg acccgatagg tcgcttcggc
    44221 cgtcttggca atcttgtccc cccgcagata gaaatggttc tgggcgacga ataccttgcc
    44281 ggcgtgaaca accccggtct tgtccgtcag atggaaggtc atccggtcgc cttcgaggac
    44341 atcccgcccg gacgtcaggc ggacgttacc ctcagcatgg gccgtgtcgg tccggtggtc
    44401 gagcaggacc gactgtgcag tcaggcggcc gccgctgtaa atgatgacga cattgccttg
    44461 ggcgtgatag gtatcggtgt cccgatcgta ggacaggctg tccgcctcaa tgtcgaccgg
    44521 tccctcccgc ggggccagtt ccatcgccag gccttgcccc cccatgccgt tcaaaacgaa
    44581 cgataaaagg gccatgagga caaagcggat ggttttcccc gtgatgccgt acatgcttgg
    44641 atcgtggcct ttccaaaccg ggtgagggtg agcctgcctg ttagcacacc ggaggagatt
    44701 tttcaaaggg agacccgccc gaagcaggct tttatttctc cgatcagata gagggagccg
    44761 gcgacgcaaa cgaggtcgcg cggaccggcg atcagcagcg ccctccgcag ggcctcccgg
    44821 ggttcttcga ccgcctccac acgggaatgc caccgcgcgg ccgccgcggc caggtgcgca
    44881 gccggaaggc cccgttcatt acggggccgg gtcgcgatga tcgtggcgtc gagggacgcc
    44941 gcctgtcgga tcatccgggc ataatcctta tccttgagga ctcccaggat caggatcagg
    45001 cgcgcatagg agaactccgt ccggagcgac cggaccagtg cggccatgcc ggcgggattg
    45061 tgggccccgt cgacgacgac gcgggggcgc tcccgcaggg tttcgagccg gccctcccag
    45121 cggacgttca gcaggcctcg tcggatcgcc gcttcgtcca gaggcacccc ccgctcggag
    45181 aggatttcgg ccgcgccgac cgccaaggcg gcattggcct gctggtgccg accggcaagg
    45241 gccaggtgca gatggcgcaa gaccgtcttg acgccgaaat agctgaacga cccgtcgtca
    45301 tgacgccgga tgcggatctc cccgcccagc cggagcagac gaacccggcg ccgccggcag
    45361 gtctccgcca aggtgtcccg gaccgccgcc tgcgttgccg ccgtcagaca aaccccgccg
    45421 gtcttgacga tccccgcctt ctcccgggca atgtcgcgca gggaccggcc cagataatcc
    45481 cggtgctcca gggagatgtt cgagatgatg ctgaccgccg gcctcacgac attggtggca
    45541 tccagacgcc ctcccatccc cacttccaga acggcccagg gaacggcttc acgggcaaaa
    45601 tgggtgaagg ccagggccgt cagaaattcg aaataggtca cctcttcggt cagggccgcc
    45661 cgcacctccc ggatcagggt ccgggcctgc gcccgggaga tgggccggcc gccgacccgg
    45721 atccgctcgc ggaaatcgat cagatggggc gacgtgtaca gaccggcccg gacgccgccc
    45781 tgcttcagaa tctccgcgag catcgccgat accgacccct ttccgttcgt ccccgccacc
    45841 agaacggtcc tgtaccgttc ctggggatcg cccagacggg cgagcagccg tttgatcggc
    45901 cccagcccaa accggatctg cgccggaccg aaggacgggg gaaaagtcgg gggggggaga
    45961 gacgtcatgg gcatgcccgg tggccggagg cctcagcagg gatcgatccg gatacgctga
    46021 ccgtccttca gaaaatggat ccggtggttc ccgatcaccg cgatctgttc gcggatgacg
    46081 tcgtggcagt cccacttcat gtggtagagg tagacttcgg ggtggggatg gcaaagcttt
    46141 ttcagttctt ccttaagcag ggcaggcgtg agatgacccg tctgcgacgc catctccttc
    46201 atgccgtccg gcagggaggt ttcgatgaag atggcccgga gatcgtccac accgttcgcc
    46261 cgctcccaga ggggttccgt gacgcccgta tctccgaaaa agatgaccgt gccctccgcc
    46321 gagcgaacga catatcccac cgtttcgacc gtgtggtcga ccttgatcgc ctcgatctgg
    46381 aggtctccca cccattccgt tttaccgggc accagggcct tgaacctaat gaccggtttc
    46441 tcccggctgg ggaggatgct gaagtcgggc cagatgacgc cgttgaagac gtgcgcgtgg
    46501 atcatctgca ggacgaaggg cgtgcttcga accgtcaggg gcttgtgccg ctccccgtaa
    46561 cagaggttgt ccgctaggaa ggcgatatcg cgaatgtgat cgaggtgcgc gtgggtcacg
    46621 aggacctgat cgatgcgccg ctgttcttcg atgttcaaga cgttcgcgac ggcgccggcg
    46681 tcgatcagca ggtggccgtt cagaagaaaa ctcgtcgtat tcaggcccgg aagctgcgat
    46741 ccatggcaac ccaagactct gatatccatg ctgttccttc gatatctccc gagacggcgg
    46801 caacgcccct gggagcgtgg gcacgtggag catcccggcg gcttggggca gatcgttcag
    46861 gcgggcgctc agtccggaat caccctgaag acctcgtccc cgggacggac gcgatcaccg
    46921 atcttcaccc cgatgaaatc acccgtcacg gccgtatcga tcttctggtt gttgacttcc
    46981 atggaatcga tggtatgcgt cagatcggtc gtatgccccg taaacttgac cgtatcgccc
    47041 accttcaact cgccgcccgt taccttgacg gcggcaacac tgggctttgc gaaaaacttc
    47101 accacttctc cgatcttgac ctctgccatg acgacacctc cttcagcgac ttgataacca
    47161 tcggcaaaga cggcagattc tcccgttccg gagcagcgtc cgggttgcga cgggagccct
    47221 gaaaccgggg gaattataat cgtttcactg gaaaatacaa gggaaaagag acccttcccg
    47281 cggcgcgcat aacaaaaaag gcagggggtg agcctgcctt tttcctgcgg aacgcaccgg
    47341 cgttctactt cttggccgcc gtctttttct ttggtttatc caccgtcacc gccgggatga
    47401 attcgatcac ggacaccggg gcattgtccc cgtggcggta gcccattttc acgatccgtg
    47461 tataaccgcc cggacgctcc cgaaagcggg gggacaactc atcgaacagc ttgatcacca
    47521 tggcccggtc ccggatgaag gagagcgcct gccgtctggc atggaggtcg ccacgctttc
    47581 ccagagtcac catgcgatcc gccagactct tcaaaacctt ggccttggcg tcggtggtcg
    47641 taatcttttc cgtgcggatc agggaggtca ccatgttgcg cagcatggcc tgacgatggc
    47701 tgctcgacct tcccagtttc ttgccaaact tcccgtgacg catacccgat ctccttctct
    47761 cccgacgcgc cgcctaatcg gcctgcgaac cgttttcact ccccttcttt tccatctcgt
    47821 tccacggggg aaagtcgagc ttcatgccga accccagccc catctccgcg agaatttcct
    47881 tgatttcatt aagcgatttt cgcccgaaat tctttgtctt gagcatttcc gcttccgttt
    47941 tctgcacgag ttcaccgatc agattgatcc cggcgttctt cagacagttg gcggagcgta
    48001 cggacagctc catgtcgtcg acgttccgca ggaggtgttc gttgataccc tcgacctccc
    48061 ccgtttcgac cgcttctccc tgctcttcca gttcctcgaa gttgatgaag acgtcaagct
    48121 gctccttgag gatcttcgac gcatacgcca cggcgtcctc cggcaggacg cttccatccg
    48181 tccagacttc cagcaccagt ttgtcgtagt cggccacctg gcccacgcgg gcataggtca
    48241 cggtgtagtt gaccttccgg atgggcgaaa acgtggcgtc gatattgatg gtcccctccg
    48301 gctgctccag atccttttcc ttctgcgccg gaacgtaccc cttgccattt tgcacaacga
    48361 gctccgcgtg gaaggcgccc tttccgaaaa gggtggcgat gtgatgctcc ggattcagga
    48421 cgtctacggt ggcgtcggcg atgatgtcgg cggcggtgat cgccccctcc tttttggtgt
    48481 cgatccggac cgtccgcggt ccgtcaccgt gcagcttcag ccggatgccc ttcaggttga
    48541 ggatgatgtc ggtcacatct tccttgacgc cgggaatggt ggaaaattca tgaaggaccc
    48601 cgtcgaaccg cacggacaca atggccgccc cctggatgga cgacagcagg accctcctca
    48661 gggcattccc cagggtgatc ccgtaccccc gctcgagagg ctgacaggtg aactcgccat
    48721 agaacggggt atgggtgccc tcatcgatct cgattcgctt cggtcggatc aaactgcgcc
    48781 agtttttctg catggtcttt cttctcccgt tcccctgatc gtccgtcgta acgtgatcgc
    48841 gctatttcga ataaagctcc acgaccagct gttcctggac cggcatggtc aaatcctcac
    48901 ggcccggcat ggtcttcacg accgcccgga agttcgtccg atccaattca agccactgcg
    48961 gtacgcctcg ccgggcaacg gtttccatcg cttcggcgat ccgctggacg ttacggctgc
    49021 cttcccgcac ctgaacctcg tcccccacct tgacctgata ggaggggatg ttcaccggct
    49081 tgccgctgac cagaaagtga ttgtgccgga ccagttgtct cgcctcggca cgggaattag
    49141 cgaagcccat gcggaacacc atgttgtcca agcgcctttc gagaccgacc aagaggttcg
    49201 ttccggtgat gcccttctgc cgatcggccc gatcgaagta gccgcggaac tgccgctcga
    49261 gcagtccgta catccgccgc agcttctgct tctccctgag ctggacgcca taatcggaaa
    49321 attttcgtcg catttggcca tgctcgcccg gcgcatatcc ccgccgctca aacgcgcact
    49381 tctcgctgta gcaccggtcc cccttgagga acagcttcag cccttctctt cggcataatc
    49441 tgcatgcgga ttctcgatac cttgccaaaa aagcctccct gcttcctgaa tgtctcgtta
    49501 cgtcccgtcg aggggagcgc cccccggcgt ttccttgtcg cttttcgccc ccggcccctt
    49561 acacgcgccg gcgtttgggc ggccggcaac cgttatgtgg gatcggtgtc acgtcccgga
    49621 tgagagtgat gttgagaccc gccagttgca acgaccgcaa cgcggactcc cggccggaac
    49681 cgggcccctt gacatacacc tggacgctcc ggagaccgtg ttccttcgcc ttcttcacgg
    49741 catcctccgc cgccatctgc gccgcaaagg gggtgctctt ccgggacccc ttgaacccct
    49801 gcatccctga ggacgcccag gcaatgacat taccgctcag atccgtgatc gtgacgatcg
    49861 tgttgttgaa ggtggactga atgtgggcca cgccctcggc aatgttcttc ttttcctttt
    49921 tctttcccgt cttcctgacc gcctttgcca tgtttcctcc ggcttgctat ttcttcttac
    49981 ccgcgatgct tctgcggggc ccttttctcg tccggccgtt ggtatgggtc ctctgccccc
    50041 ggacgggcag ccccttgcgg tgccgcagac cccgataggt tccgatgtcc atcaggcgct
    50101 tgatggacat ggatacttcg cgccggagat caccctcgac cttctgctcc cggtcgatga
    50161 cattccggat cgcgttgatc tccatatcgg ccagttcgtc ggtcttggtg ctttcgctta
    50221 cgccggcctt ggctaaaatc tgcttcgccc gcgatcgccc gatcccgtaa atataggtca
    50281 gggcaatctc catgcgcttg ttcttgggta aatcgacacc tgcaattctg gccacctgat
    50341 aacctcctta tggatcccgg gcatctatcc ctggcgctgc ttgtgtttcg gattctcgca
    50401 gatcacccgt aaaacgcctc gccgcttgat gattttgcac ttgtcacaga ttttcttgac
    50461 ggaagatcgt actttcactg ccaacactcc ttcgcttctc cggccgaagc gaccgccgcg
    50521 gttcttccgc ccggtcgccc gccgctgatt ccggacggct acttcgtccg gtacgtaatc
    50581 cggccccgcg tgaggtcata gggggaaagc tccaccgtaa ccttgtctcc gggcagaatc
    50641 ttgatgaagt gcatccgcat cttcccggag atatgggcca gtacgcgatg accgttttcg
    50701 agctcgacgc ggaacatcgc attgggcaac ggctcaatga ccctcccttc gacctcgatc
    50761 ggttcttcct tcgccatggt tttcggtctc cctctcccgc gcatccccgt gttagccgga
    50821 cgccggtcct tccgtcacgg tcccgccccc tcagcgcagc aggctcagga tctccggccc
    50881 ccgatccgta atcgcgaccg aatgttcaaa atgcgccgac aactttccgt ccgccgtcac
    50941 caccgtccat ccgtcttcca agacctggac acgataggtc ccgagattga ccatcggttc
    51001 gatggcgagg accatgcctg ctttcagcgc caccccgcgg cccttgacac cgtaattcgg
    51061 aatctgaggg tcctcgtgga gctcccggcc gatcccgtgt ccgacataat cccggacgac
    51121 ggagcagccg gccgcctcga cacacgcctg gacggcggcg gagatgtctc ccaagcggtt
    51181 tcccgccctg gcctccctga tcccctcgta gagcccggct tcagtcacgt ccatcaagcg
    51241 acgggcctcc cgcgaaacct gcccgatcgc gaccgtgatg gcggcatcgc cgaaataccc
    51301 ctgataatcg acgccaaaat ccaggctgac gagatcgccg tccttcagaa cacggccgtc
    51361 ggagggcatc ccatgcacga cctcctggtt gaccgacgca cagagggaat agggataacc
    51421 ccgatacccc ttgaacgcgg gtcgcgcgcc ccggcgatgc gtgagcgatt ccgagaggcg
    51481 atccaagtcg cccgtcgtca cgccgggctt ggcgtgcgcc ttcaattccg caaggatttc
    51541 agccacgatc cggttgctct tccggatctt ctcgatttcc tccggtgatt tcaggatgac
    51601 catgtcattt tatctgccgg acggcacgcc tcccgtcagc gccttctggc aatgcgtcct
    51661 ttcttcatga agccttcgta ctgccgggtg agcagatggg tttcaatctg accggccgta
    51721 tccagggcaa cacccacgac gatcaggagg gccgtcccgc cgaagtaaaa cgggacgttg
    51781 aaataactga tcaggatgct gggcagaacg cagaccgctg aaacgtagat tgctccgctg
    51841 aaggtcagcc gggtcaacac gtcgtcgatg tactccgccg tctttttccc cggccgtatc
    51901 cccggaacgt atccgccgta cttcttcatg ttgtctgcca cgtccaccgg attgaaggtg
    51961 accgccgtat agaagtagca aaagaacacg atgaacgcga catagagcag ctcatagccc
    52021 caccgccccg gcaccatcat atcggcgacg gtcttcatcc agggatgggg gatgaaattg
    52081 gcgatcgtcg ccggaaacat gatgatcgag gaggcgaaga tcggcgggat cactccggct
    52141 gtattcacct tgaggggcag atgcgtcgac tgcccgccgt agaccttccg gcccaccacc
    52201 cgcttggcat actgaaccgg gatccggcgc tggccgctct ccacgaagac gatgaccccc
    52261 accaccgcga ccatcatgag gatcagcagg ataatgaaga agaaccccat ctcgcccgaa
    52321 gaaagaaggc gaaacgtgtt cagaatcgct tccggtccgc ggcagacgat cccggcaaag
    52381 atgatcaggg aaatgccgtt gccgatccct ttctcggtga tggtctcgcc cagccacatg
    52441 atgaaggccg taccggccgt gagggtgatc accgtcatga gccggaagga ccagccggcg
    52501 gtgatcacga tggaagcccc cgtcggaccg gccatgttct cgaggccgat ggcgatcccg
    52561 aacccctgga taatgctgag cagaaccgtc ccgtagcgcg tgtactgggt gatcttgcgt
    52621 ctccccgcct cgccttcctt cgagagccgc tccaggtggg gaacagccac cgtcagcagc
    52681 tgcaaaatga tggaggcgct gatgtagggc atgatcccca gggcaaaaac ggagaggttg
    52741 ctcagcgcgc cgccggcaaa catgtcaaag agccccagca gggatccctt ggcctgctcg
    52801 aaaaaggcgc ccagggccac ggtgtcgatc ccgggcgtgg ggatatgagc gccgatcctg
    52861 tacacggtca gcaaaacgaa cgtgaagaga atccgttttt gcagctccgg gatcttctgg
    52921 atgttcttta aaccgtctaa caactcatgc cacctcgaca acggagccgc cggcggcctc
    52981 gatcttggcc tttgcgccct ggctgacctg atccaccttc agggtcaggg gaccggtcaa
    53041 ctcacccttg cccaggattt tcacgccgtc gttctttttt cggatcaatc cacaggccac
    53101 cagcatatcc ttgtcgatga cggaaccggc gggaaagcgc ttgagctgat cgagattcag
    53161 ggtcgccaag acccgacgaa acggattctt gaagccccgc ttcggcaggc ggcgcgccat
    53221 gggcatctgc ccgccttcga acgccggctt cgtcttgccg ccggaacggg cattctgtcc
    53281 tttggcgcct ttacaggcgg tcccgccgtg tccggagccg tcaccccgcc cgacgcgctt
    53341 tctcttcttt cgagacccct ctgggggttt cagttgactt aaattcatcc ctgtgcgcct
    53401 ccttcgaggt cctcaaccgc cacgaggtga atgaccttct ggaccatgcc gcggatggcc
    53461 gacgtatcct cgagcacgac ggaccggttc attcgtccga gccccaggcc gcgcaggact
    53521 tgccgctgct tcgtcggccg gccgatcata ctttttacga gcgttatctt cagttttttt
    53581 gccacaaccg acccctcact tccctacgct tccccgaaac tcttcccccg caactggaaa
    53641 atggttgcgg gatccttcag ttggaccagc ccatccagcg tggccttgac caagttatgg
    53701 gggttgtggg agccgaggca cttggtcagg atgttgtgta tccccgccac ctcgagaacg
    53761 gcccggacgg caccgcccgc gatcagcccg gtcccctccg aggccggctt gagcagaacc
    53821 cggccggcgc cgtagcgccc caccacttcg taggggatgg tccgctctcg gatggacacc
    53881 ttcaccatat tcttctttgc ctgctccatg cccttacgaa tggcctcggg aacttcatga
    53941 gcctttccca gacccgcacc cacggagccc tgtccgtcac ctacgacgac gacggcgctg
    54001 aaacgaaatc gtctcccccc cttgaccacc ttcgccgtgc ggttaatcgc gatgaccctg
    54061 tccagcagtt cctgttcttc catgtctcgt cccgtcacca ttcctgtgag atattgcgcg
    54121 cgccgcacat cagaatttca ggcccgcttc ccgggccgcg tccgccagcg ccttcacgcg
    54181 gccgtggtag agataccgcc cgcgatcaaa aaccacctgc tcgaccccgg cggctttcag
    54241 ccgctcggaa accagcttgc ctacctcgcg ggccgcttcg ctcttcttca tccgctccaa
    54301 acgctctttc agctccgggc tcagggtgga cgcagcggcc agcgtttggc cgacgtcgtc
    54361 ggccacggcc tggacataga tatgccgggt gctccggaac acggacagcc tcggaacggc
    54421 ccgggtgccc ttgatctgcg acctcaccct cgcctcacgt ttcgccctga tcttttcaat
    54481 cttctttgtt gccaatgttc tacctcatga tccagcgcat ccgggcagcc ccgcccgccg
    54541 tttcttgatt cccttaggca cccacggatt tccccacctt gcggcggacg tactcgccgg
    54601 cgtacttgat gcccttaccc ttgtacggtt ccggtttccg gataccgcgg atctccgccg
    54661 ccacccggcc gacctgctct cgatcgattc cggagaccga gatgttgacc tgcttttcca
    54721 cgctgacctt gacgcccggc ggcacctgat aggttatggg gccggagaaa ccgaccagaa
    54781 gcttgagggc atccccggcc atctccgcgc ggtagccaac gccgacaacc tccagattct
    54841 tgacaaagcc cgtgctgacg ccggttacca tgttggcgat cagcgtgcgg gtcagtccct
    54901 gataggcgtc acccgtccgg gtatcctcga gcttcgtgac cgtgattgtc cggtcgtcga
    54961 tccccacctg gatccccgcg ggcagggttc ttgacagcgc tcccttcggc ccctcgaccg
    55021 tgatccgctc ctgatcccag cgcacctgga cgcgctcagg aatttcgatt gctttctttc
    55081 cgattctcga catggttcat ctcccgtcgg gtggccctgc taccagactt tgcacaggat
    55141 ctcgcccccg acattcagct cgcgggcctc ctgatccgtc acgattcccc gggatgtgga
    55201 aagaatggag attccgtaac cgttcagaac cttgggtatc tctccgcttc ccacatagat
    55261 ccttcggccc ggcttgctga cccgttgaat gtccgtcatg acggtccgtt tggcatccac
    55321 gtacttgagg aatattctca gcacttggcg gccctgggcg ttcttcttga catcgtagcc
    55381 gctgatatac ccggacttct tcagaacctt ggcgacgctc aggttcatcc gggaaagcgc
    55441 cacatcggtg cttttgaaat gggcgcgatt ggcatttcgg attcttgtaa gcatgtccgc
    55501 aatcggatcc gtcatcccca taactgactc cttaattccc tgctgactac cagctcgatt
    55561 tgatcacacc gggtatctcg cctttcgagg cgaggtttct caagcatatt ctgcacatat
    55621 cgaatttccg gtaatacgcg cgcggccgac cgcagacggc gcaccggttg taagcacgta
    55681 cggaaaactt cggttttctt gctgcctttg caatcaagga tttcttcgcc acacccatct
    55741 cctccggatt tcgtgttcct ctcgttccgc ttgacgtcct aacttcggaa gggtatcccc
    55801 atgagcttca gaagcagtcg cgcctcttcg tctgttttgg cggtcgtgac aatggcgatg
    55861 ttcataccct tgaccttatc aatcttgtcg tagtcgatct ccgggaagat gatctgctcc
    55921 ctcaggccca gggaaaagtt tccgcgccca tcgaaggatt tcagtgaaat cccccgaaag
    55981 tccctgatcc ggggaagggc cacgttcgcc aagcggtcga agaactcata catgcgctcc
    56041 cgccgcaggg taacgcgaca gccgatggac attccctggc gaagcttgaa ggtggcgatg
    56101 gacttcctcg ccttggtgat gaccggcttc tggccgcaga tcgccgccat ttcctgggcc
    56161 gcactctcca ggatcttcac gttctggatg gcctcgccca gccccatgtt gatgacgatc
    56221 ttttccagtt tcggaacctg catccggttc tggtacttaa acgcctcgat cagggcggga
    56281 acgacctctt tttcgtagta actcttcaat ctcgctgcca tgattccatt atcctaagcg
    56341 tccaaaatct cttggcactt cccgcagacg cggaccttct tgccatcgtc caggagccga
    56401 tacccgaccc gcaccgtccc gtcgcatttg ttgcagacga acatcacatt ggaaaggtgg
    56461 atcgagccct ccttttccac gatgccccct ttgcccttcg catcgggccg ctggtgccgt
    56521 ttgagcatgt tgaccttctc gacgatcacc cgctctctgt cccctacgat cttcaggatc
    56581 ttgccggttt tccctttttc cttcccagcg atcaccttga ccgtgtcgcc ctttttcaac
    56641 ctgatgtcct gcatctttct tcctcgcctt tagagcacct cgggtgccag agagatgatc
    56701 ttcatgaact gtttggcccg tagttcccgt gcgaccgggc cgaagattcg ggtcccgacg
    56761 ggctccaact gattggtaat caggacggcc gaattgtcat cgaacttgag ataggatccg
    56821 tccggtcgcc gaacctcttt caccgtccgg acgatcacgg ccttcatgac gtcccccttc
    56881 ttgaccttgg aattggggag cgcctccttc accgaaacca cgatgacgtc tcccaggctg
    56941 gcatagcgcc gcttcgatcc gcccaaaacc ttgatacagg cgaccttctt ggcccctgaa
    57001 ttatccgcca cattcaagac ggtctgcatc tgaatcatga ctacaccccg ctgaaactcc
    57061 ccgctcaatc cgcaccaggc aggaaggtcc cccgcctgcc tggccttctt tgaaggcaaa
    57121 cgccgcgcct acttcgcccg ttccaggact tccgtcatcc gccaccgctt ttcgcgactc
    57181 aacggccgac actccacgat cagcacccgg tcaccgaccg cacactggtt ggtctcatcg
    57241 tgggccttgt acttttcgta ccgtttgatg tatttttgaa aaaccgggtg cttgacaatc
    57301 ctctcggcac ggacgacgat ggttttgtcc atcctgttgc tcaagaccac cccctggatg
    57361 gtttttctgt tcccccgctc gctcatgcct tttgctcctc cttcaccctc atgatggtct
    57421 tgacacgggc gatgtcgcgc cgcacccgcc gcatctgggc gggggattcg agctgaccgg
    57481 aggcatgccg caggcgaagc ttgaagagct cctctcccaa ctcgcgatcc cgctgctgca
    57541 gttcagccca actcaggtcc cggatctcct taatcttcat cagacaactc cctggccaga
    57601 aacttcgtca caatcggcag cttatgcgcg gcaaggcgga aggcttcacg ggcaacggcc
    57661 cggtccaccc cctccatttc gtaaaggacc atgcccggcc ggatgacggc cacccactct
    57721 tccggggcgc ccttcccttt gcccatacgc gtttccgccg gcttcttggt cacggatttg
    57781 tgcgggaaaa tgcggatcca gatcttgcca ccccgcttga catgacgggt catcgcgaca
    57841 cgcgccgcct cgatctgtct tgcggtgatc cacccacact ccatcgcctg gaggccgtac
    57901 tcgccaaagg tcaccttggc gcccttggtg gatttgcccc ccatgcgtcc ccgctgtaat
    57961 ttcctgtatt tcacccgttt cggcattaac atcgtcagct actcctgttc tcctcccccg
    58021 aatctctccg cccaccgggg cgggaggcac gcgggcctat tccttgctcc gctttccggg
    58081 caacacctcg ccgtggaaca gcagcacctt gacgccgatc gcgccatacg cggtccgggc
    58141 ttcggcaaaa ccgtagtcga tatccgcccg gagggtgtga agcggaacgc gcccttcacg
    58201 gtaccactcg gagcgggcca tctccgcccc gccaagccgc ccggcgcaac tgatccggat
    58261 ccccttggcg ccgaacttca gggccgaggt cacgcacttc ttcatcgccc gccgaaacgc
    58321 gacgcggcgc tcgagttggc tggcgacgtt ctccgccacc agttgggcat ccacctcggg
    58381 ttttcggacc tcaagaatat tgatgatcac ctcgctgcgg gtgatcccct cgagatcctt
    58441 cttgagcttt tcaatctcgg cgcctttctt gccgatgatc acccccggcc gagcggcgta
    58501 gatgttgatc ttcgcctttt gggcggcacg ctcgatttcg atcttggaga tgccggcatg
    58561 atagagtttc tctttcagaa acttcttgac cttcaagtct tcgtggagga gttcggcgta
    58621 gttcttctcc gaaaaccatt gagaccccca tgtcttgatg ccccctagcc tgaacccgat
    58681 ggggttgacc ttctgaccca acctgacacc tcctcaacga tcaacgttca tccaagataa
    58741 cggtaatgtg actcatccgc ttgcgtatgc tggccgccct tccctgggcc cgcgcccgcc
    58801 accgcttcag cgacggcccc tgatcgacat agacggcctt cacgaaaaga atcttctcgt
    58861 cgatgttcgg gttctgcgtc gcgttcgcca gagccgattt caagaccttc ttgaccatcc
    58921 ccgcggcacg cttgcgggtg aacgccaaga tggtcgccgc ctcctcgacg ggtttccccc
    58981 ggaccagatc caccaccacc cgaaccttct gtggtgagat gcgcacatat tttgcaaccg
    59041 ctctcgattc catatcccct ttatactcca cccttacaat cgtccctaca actgcctccc
    59101 gggaaatcgc ccggattcgg cggttatcgc ttcaccttgg acttccggtc gccggcatgg
    59161 ctgaaaaagg ttcgcgtcgg ggcgaattcg cccaatttat gcccgaccat gttctccgtc
    59221 acaaacacgg gaatgaactt cttgccgttg tgaaccgcaa aggtttggcc gatcagttcc
    59281 ggggtaatcg tcgaacgccg ggaccacgtc ttgatgaccg ccttgctgcc cgaagcctcc
    59341 gccttgcgga ccttatccag aagcttggct tcgatatacg gaccttttct gattgagcgc
    59401 gccacttctt aaccccttct cttgacgatg tatttatccg tccccttgtt cgttcgcgtc
    59461 ttgtgaccct tggtcggcac tccccaaggc gtgcagggat gccttccacc cgaggatttg
    59521 ccttctccac cccccatcgg atggtcgatc gggttcatgg ccacacctcg caccttgggc
    59581 cgtcggccct gccagcgggc gcgcccggcc ttcccgatgc tgatgttctc atggtccaga
    59641 ttgccgacct gaccgatggt cgccgaacac tcgatgaaga ctttccggac ctctccggac
    59701 ggaaggcgga cctgggcgta tcccccctcc ttggccatga gctgcccgta ggcgccggca
    59761 ctgcggatca actgcccgcc cctgccgacc ttcagctcga cgttgtggat gagcgatccc
    59821 aaggggatgt tcttgagcgg cagggtattg cccggcttga catccgcccg ctcggcactc
    59881 accaccgtat cccccacttt cagcttgtcg ggtgcgagga tgtatcgttt ctcgccgtcc
    59941 agatagttga gcagggcgat cctcgccgac cggttcggat cgtactcgat cgaggccacc
    60001 cgggccggga catcccgctt ttcccggcaa aaatccacaa ggcggaattt tcgcttgtga
    60061 ccgccccccc tgtgccggct cgtgacccgg ccgttgctgt tccgcccacc ggttttattg
    60121 agcgaacgga cgagaccctt ctcgggcgtc gccgaggtaa tgtccttgaa gtccgagcag
    60181 gtctgaaacc gccggcccgg cgatgtcggt ttgtatttct taatcatcgg ctttcttcct
    60241 tgacgtcttt tcgtcctgcg cctgactaaa ccccctcgta cagctcgatg ctgttccccg
    60301 gcgcgagggt cacgatggcc tttttccagt tctgcttctg cccgacgatc cgtccgaggc
    60361 gctttttctt gcccgtcatg ttcaggacat ggacgttgac gaccttgacc ttgaacagct
    60421 tctccaccgc atcgccgatc tcgatcttgt tggccttccg gttgacctcg aagacgtact
    60481 tgttggcctc gtccttggcg atggtactct tctccgtcag gtgcgctctt ttgatgatcc
    60541 ggtatcggtc cattatgaca acaaagcctc ttcaatcttt ttcacggagg gttccagaag
    60601 aacgacccgg tcgtatttga gcagatcgta aacattgatc ccttccgacc ggatcatttt
    60661 gacgtccttg atgttccggg aagacttctc caggatcggg ctggcctgat cgatgacgaa
    60721 cagggccttc ttcagtccca ggcgatcgag gacggactgg aaccgcttcg ttttgatctc
    60781 ttccatggga atgtcccgca ggatgatcat ctgcgcttcc tggaacttca tgctgagcgc
    60841 cgagatcaga gcaagacgcc gcatcttctt gggcatgcgg aacgcatagc tgcggggttg
    60901 cggaccgaaa accgtcccgc cgcctttcca gagcggggag cgggtcgttc cggcccgggc
    60961 gcggcccgta cccttctggc gccagggctt cttgccgccg ccgctgacgt cgctgcgccc
    61021 cttggtcgcc gccgttcccc ggcgccgggc cgcctgctgc atcctcacga catcgtaaat
    61081 caggctttca ttgacggagg cgccgaaaac cgcttcgctc aacgtgatct ccgaaatttt
    61141 ctcgttttca atattgtaaa ccgctgccgt cggcatcttc accacccgtt cctgtccagt
    61201 tcctacttct tgatcgcctt cttcaccaaa agtacgcccg ccttgccccc gggaaccgcc
    61261 cccttaacga ggatcacatt ccggtccggc cgcacatcca tgaccatcag attctgaacg
    61321 gtctttcgga cgtcacccag ttgcccggga aggcgggtcc ccttgaagac ccgggaggga
    61381 taggcgctgg cgccgatgga gccgggtgcc cggtggaaca tggacccgtg cgtggcgcga
    61441 ccgccgccga agccgtgtcg cttcatgacg ccggcgaatc ccttcccctt ggagatgccc
    61501 acgacatcca cgtaatcgcc gatggcaaac gtctccgccg tcagatcctg gccgggctcg
    61561 tagccctccg gagagatgcg aaattccctc agatgacgga aaaagccttt gcccgccttc
    61621 aggagatgcc cctgcagggg tttggtgacg ttcttctgtt tgatgcgtcc gtaaccaagc
    61681 tgaagggcgt cgtagccgtc cgttttgacg gttttcttct gaatcacgac ggacggctcc
    61741 atctcgatga ccgttacggg gacggctgcg ccatcctcgg taaagatctg cgtcattccc
    61801 aactttcttc cgataagccc tgctttcatg gtcgttccag tatctttcct aaaatttcgg
    61861 aaaaccgccc cgagagaaaa aaacggagga gatgaatcct gcatccatct ccccgtacgc
    61921 ctaccctaca acttgatttc cacatccacc ccggcggaca gatccagctt catgagtgcg
    61981 tcgaccgtgg cctgggtcgg ttcgatgatg tcgatcagcc gcttgtgggt tctaatctcg
    62041 aactgctccc gggacttctt atcaacgtga ggagaacggt tgacgcagaa cttgttgatc
    62101 tccgtcggca gcggcacggg acccgccaca cgggcgcccg ttcgtttggc tgtctcgacg
    62161 atgtccacga ccgagcggtc cagcagcttg taatcgtagg ccttcaggcg tatccgaatc
    62221 ttttgatctt tcatggtctc ttcgtttcca ttcgctcagc ggcttactcg atgatcttgc
    62281 tgaccacacc ggcgcccacg gtccggccgc cctcgcggat ggcgaagcgc agctgctctt
    62341 ccatggcgat cggggtaatc aggttgatct ccaggtggac gttgtcgccg ggcatgacca
    62401 tctccacgcc ttcgggcagc gtcgtcaccc cggtcacgtc cgtggtccgg aagtagaact
    62461 ggggacggta cccgttgaag aacggcgtat gacggccgcc ttcctccttc gtcaggacgt
    62521 aaacggcagc ctcgaacttg gtgtggggag tgatggaatt cggcttggcg acaacctggc
    62581 ctcgttccac gtcttcccgc ttggtcccgc gcagcaggac gccgatatcg tcacccgcac
    62641 gcccttcatc cagggttttg cggaacatct cgaccccggt gcagaccgtt ttgaacgtgg
    62701 gccggatccc gacgatctcg acctcgtcgc cggtccgaac gatcccgcgg tccacacgac
    62761 ccgtcacgac ggtgccgcgg ccggagatgg tgaaaacgtc gccgatcggc atgaggaagg
    62821 gcttgtccag gtcgcggacg ggctcgggaa cgtacgtatc gacggcatcc atcagcttga
    62881 agatgctctg aacgtcggag gcgttctcgt cgtcgctctc gagggccttg agggcggaac
    62941 cgcggatgat cggaatttct tcaccgggga attcgtaagt cgtcagcaat tccctgagtt
    63001 ccagctccac gagatccagg agttccggat cgtcgacgag atcgaccttg ttcatgtaaa
    63061 ccacgatgta ggggacctgc acctggcggg cgaggaggat gtgttcccgg gtctggggca
    63121 tggggccgtc ggaggcggcg acgacgagga tcgtcccatc catgtgggcg gcgccggaga
    63181 tcatgttctt gatatagtcc gcgtggcccg gacagtccac atgggcgtag tggcgcttgg
    63241 ccgtctcgta ttccacgtgc gagatattga tcgtcacgcc tctttccttc tcttccgggg
    63301 cgctgtcgat ggaatcgaac gacctgaact cggccagacc tttcttggac aggtgcttcg
    63361 taatggctgc cgtcaacgtg gtcttgccgt gatccacgtg cccgatggtc ccgatattca
    63421 gatgcggctt cgtcctttca aatttcttct tcgccattgc tgttacctcc tcctataact
    63481 ttgaatggtt tttatccgta ttgcccgagc gcctaacagc agcctcttcg ttcatccacc
    63541 ctagaaccga taatgggcaa aggccttgtt cgcctccgcc atcttgtgaa cggcttcctt
    63601 cttcttgatg gaagcgcccc ggttgttggc cgcatcgatc agctccgctg ccagtttctc
    63661 ccgcatggtc ttctccgtcc gctccttgga atggaggatc agccagcgca acgccagcgc
    63721 catccgccgt gacggagcca cctccgtcgg cacctgatag gtggatccgc cgacgcgccg
    63781 ggatttgact tcgatgacgg gcttcacatt gttcaccgcc cgttcgaaca catcgaccgg
    63841 ctgctccttc gtccgcttct cgatgatgtc cagcgccccg tacataatgg attcggccgt
    63901 acttttcttg ccgcgcttca gcaggctgtt gatgaacttc gccaccagct tgttatggta
    63961 tttgggatcc ggcaggacct cacgcttggc aatttctctt cttctcggca tggttctctc
    64021 tccgcctagc tcggcttctt cgccccgtac ttcgaccgcc cttgtttccg gtcctggaca
    64081 ccgacggcat ccagcgtccc acgaacgatg tggtaccgga cgccgggaag gtccttgata
    64141 cgcccccctc ttacgagcac cacggagtgt tcctggaggt tgtggccgat gccgggaatg
    64201 tacgacgaga cctcgtaacc gctggtcaaa cgcaccctcg ccaccttccg caaggccgag
    64261 ttcggtttct tgggcgtcgt ggtatagact cgcacgcaca cgcctcgccg ctggggcgac
    64321 tggactaaag ccagcgtcgc cgcctttttc tggatcttct ctcttccttt acggaccaac
    64381 tgattgatgg tgggcatgat tcctctccgt aggactcgcg ctgcatttca caggtgcagc
    64441 aaaatttgag tcatctatca actcaaggcc gggctgtcaa gacttttctc gctttcatcg
    64501 ccggattctt caggtatttc agagatgggg agctcctcca ccgccgtctt gatcccgagc
    64561 ttccggtaca gggacagccc cgtgcccgcc gggatcaacc gtcccataat gacattctcc
    64621 ttgagccccc gcaagtagtc ggtcttcccc gcgaggctgg cctccgtcag gacccgggtc
    64681 gtctcctgga aggacgcggc cgagatgaag ctctgtgtgg agagcgaagc cttcgtgatc
    64741 cccagcagga gcgcctccgc cgtcgccgga cgcccaccct cggccatcat ccgctcgttc
    64801 tcctcctgaa agcggtgccg ttccacctgc tcgtcggcga tgaatgccgt gtcgcccgga
    64861 tcgatgatct tgacccgccg gagcatctgg cggacaatga cctccatgtg cttgtcgttg
    64921 atcttgacgc cctgaagccg gtagacctcc tggacctcgt caacgagata ccgggccagt
    64981 tccttttccc ccttgatcgt caagaggtcg tggggattgt ggaccccgtc catcaaggcc
    65041 tcaccggccc gcacgcggtc cccctcctgg acgctgacat gcttcccctt ggggatcagg
    65101 tattcctttt cctccccgat atcgggactg atcaccacct tgcgcttccc cttggcgtcc
    65161 tttccgtagg agacgatgcc gtcgatctcg ctgatgacgg caaactcctt cggtttgcgg
    65221 gcttcgaaaa gctcggccac gcggggaagg ccgccggtga tgtccttggt cttggtcgtt
    65281 tcgcgcggga tcttcgcgat gatgtcgccg gcgctgaccg cgtccccctc ggaggctacg
    65341 atattggccc cgacggacag caggtggcgg gccaccatgt tcgtccccgg caccttggcc
    65401 gttttgccct tggcgtcctt gatcgacacg cgcggcctca gatcggttcc ccgggactcc
    65461 acgatcacct tccgggaaag gccggtgacc tcatcgacct gttcctgcat ggtcttgcct
    65521 tcgatgatgt ccccgtactt gatggtcccg tcgacttcgg cgaggatggg aatggagaag
    65581 ggatcccatt cggcgatcag gttccccttc ttcaccacgc tgccgtcgga tttcttcaga
    65641 tgggcgccgt aaggaatggg gtacttgccg cgcccccgcc cctgttcatc gagaacgtgc
    65701 acctctccgt tgcggttcat cacgacgaga tcccccgtct tgctcaaaac cgtgttcagg
    65761 ttcatgaacc ggatcttacc gtcgtgtctc gcctccaggg tggaatgttc ctcgaatttc
    65821 gccgttccgc cgatatggaa cgtccgcatg gtcagctggg ttcccggctc gccgatggac
    65881 tgggcggcga tgatccccac cgcctccccg atgttgacaa ggtgcccgtg ggccaagtcc
    65941 cggccgtaac aggccgcgca gacgccgttc ttggagcggc aggtcagaac cgaacggatg
    66001 ttgacgcgct cgatgcccgc atggtcaatc ttttccgcca gcgcctcgtc gatcccttcg
    66061 ttggctcgaa ccaggacctc gccggtaaac ggatcctgaa tatcctccag ggccacccgc
    66121 cccagaaccc gctctccggc ggtttcgatg atctccccgc cttccatcag ggccgacacg
    66181 tagatcccgt ccagggtgcc acaatcctct tcggtgatga tgcagtcctg ggccacatcg
    66241 acaagccggc gcgtcagata ccccgaattc gcggtcttga gggcggtatc ggccagcccc
    66301 tttcgggcgc cgtgggtcga gatgaagtac tgcaacaccg tcaacccttc ccggaagttc
    66361 gccgtgatgg gcgtctcgat gatctcgccg gacggcttag ccatcaatcc tcgcatgccg
    66421 gccagctggc gaatctgcac cgcgctgccc cgcgcaccgg aatcggccat catgtaaatc
    66481 gggttgaagc tctcgatttt ctttcccgcc gcgctgtcca cgtccaccga actgatgccg
    66541 ctcatcatct ccgcggcgat cttctccgtg gcctgggccc agatgtcgat caccttgttg
    66601 tagcgctcgc cgtccgtaat gagaccgtcc atgtactgcc gctggatctt taggacgtcc
    66661 cgatcggcct tggccacgat gtccttcttg ttctcaggaa ccaccatgtt gtgaatggcg
    66721 atggacgatc cggaacgggt ggcgtacctg aaaccgagat ccttcaggcg gtccgccaga
    66781 agcacggtcg tcttgtcgcc gcagcaccgg tagcagtaat cgatcaggtt ggccagttcc
    66841 ttcttgttca tcaccttgtt gatctgatcg aaggtgatgg tgtcggggat gatctcgtaa
    66901 agcacaatcc ggccgacggt cgaatccttc acctggccgt cgacgcggat cttgatccgg
    66961 gcgtggaggt ccacttcccc cgcatccagt gcggaccgca cctcctccgg ccccgaaaag
    67021 accataccct ctcccttgac cgcgttgtcc gcccgggtca ggtaatagat ccccagcacg
    67081 atatcctgac tgggaatgat gatcggtttg ccgttggccg gcgacaggat gttgttcgtg
    67141 gacatcatga gcacccgggc ctccgtctgg gcctcgatgg aaaggggcac atgcacggcc
    67201 atctggtccc cgtcgaagtc cgcgttgaag gccgtgcaga ccaggggatg gagctgaatc
    67261 gccttgccct cgatcagcac gggttcgaag gcctggatcc ccagacgatg gagggtcggt
    67321 gcgcggttca gcatgaccgg atattcccgg accacctcgt cgagggcgtc ccagacctcc
    67381 gcggtttcct tttccacgag tttcttggcg ctcttgaccg tggtcacgta acccttgtcc
    67441 atgagccgat tgtagacgaa cggcttgaac agctcgaggg ccatcttctt cggcaggccg
    67501 cactggtgca gccgcagatc cggacccacg gtaatgacgg accggccgga atagtccacc
    67561 cgcttgccca aaaggttctg gcggaagcgg ccctgcttgc ccttcagcat atcggacagg
    67621 gagcgcagcg gacgtttgtt ggtcccggta atggcgcgcc cacggcgccc gttgtcgaac
    67681 agcacgtcca cggcctcttg gagcatccgc ttttcgttac ggatgatgat ctccggcgcg
    67741 ttcagctcct gaaggcgctt caggcggttg ttccggttga tgacccggcg gtagaggtcg
    67801 ttgaggtcgg acgtggcgaa acgcccgccg tccaggggaa cgaggggacg gagatccggc
    67861 ggaatgaccg gaagaactgt caggatcatc cactccggct tgttgtccga tttcctcagc
    67921 gcctcgacca ccttgagacg cttgatcatc ttcttgcgct tcgtgtccga ggtcgaggtc
    67981 ctcaattccg tgcggagctc ctcgtagagc gcctccaggt cgatctgctg gaggagtttg
    68041 cggatggctt cggcaccgat ccccgcttcg aaggcgtccg gcccgtactg ttccttgagg
    68101 tcgatgtaat cctcttcggt cagcagttcc ttcttcctca ggggggtgtt cttcggctcc
    68161 agcacgatcc aggattcgaa atagaggacc ttctccagct ccttcagcgt caggtccagg
    68221 acattgccga tccggctggg caggctcttc agaaaccaga tgtgggccac cggcgtcgcc
    68281 agggtgatgt gacccatccg ctcgcgacgg accttggact gaatgacctc cacgccacat
    68341 ttctcgcaca cgaccccgcg gtgcttcatc cgtttatatc gaccgcagag gcattcgtaa
    68401 tccttcaccg gtccgaagat cttggcacag aaaagaccgt cccgctccgg tttgaaggtc
    68461 cgatagttga tcgtttccgg cttcttgacc tcgccgtggg accaggaaag aatcttctcc
    68521 ggcgaggcaa tggacatctt gatggcattg aagcggatgg gatccttcgg tttttcaaag
    68581 taactgaaaa cgtcttccac ggacttatct cctgatggtt gtcattccgg gacgccgacg
    68641 gcggcggccc cttccttatt cttcctcgat cagatcgacg tcgaggcaga ggctctgcag
    68701 ttccttcacc agcacgttga aggactccgg aagcccgggc tccagggtat gttccccctt
    68761 gacgattgtc tcatagatcc gcgtccggcc ggccacatcg tcggatttga ccgtcaggaa
    68821 ctcctgcagg gagtaggccg ccccgtaggc ttccatggcc cagacctcca tctcccccag
    68881 ccgttgccca ccgaactgcg ccttgccgcc caagggctgc tgggtcacca gggagtacgg
    68941 cccgatggag cgggcgtgga tcttgttgtc gaccagatga tgcagcttca gcatatagat
    69001 catgccgacg gtgatttcct gatcgaacgg ttcgcccgtc cggccgtcgt agagcaccgt
    69061 ctggcccttc tcgggaagcc ccgcctgggc gagcatcgcc ttgatccgct cttcgtcggc
    69121 gccgtcaaag acgggactcg ccatgggaat gcccttcccc agacggcgca ccagcttcag
    69181 cagggattct tcgttaagcc cctccaccag gcgcgagatt tcgggagatt cgtaaatatc
    69241 cttcagttcc tttttcaggc gctttacacc gacattctcg tcgagcatgg cgcccagttt
    69301 gtcccccagg cctttggccg cccagcccaa gtgcgtctcg aggatctgtc ccacattcat
    69361 ccgggacggc acacccaggg gattcaggac gatgtccacc ggcgttccgt cggcgaaata
    69421 gggcatgtcc tcttcgggca gaatccgcga gaggaccccc ttgttaccgt ggcgtccggc
    69481 catcttgtcc ccgacggaca gctttctctt gatcgcgaca tagaccttga cgagcttgat
    69541 gaccccgggc gggagttcgt cgcccgcctt gagcttctcg attttgccgg cgaagtaggc
    69601 gtggacgaac tcgactttcc gctccagatt cgtcagcagg gtggcgatgc gctcttccag
    69661 ggcctcctcg tcctgcagcg ggatttcctt ccagagttcg aaggggatct tctccaaaac
    69721 ctcttcggtc acctcatcgc ccttcttcag gacgatcttc cgttttttgg ggtccgacag
    69781 ttttcccgcc gcggtcttcc cgacgatcag ggaacgaatc tccttgcgaa cgctgtcggt
    69841 gatgatccgg atctcgtcgt cccgatcttt ctccagttcg gccagagcct cgtcctcgat
    69901 gaactgggag cggtgatctc gctccgcccc cttgcgggtg aagatcttcg catcgatcac
    69961 cgtcccttcg acgccgggcg gaacgcggag ggaggtgtct cgcacctcgc tcgccttctc
    70021 gccgaagatg gcccggagca gtttctcttc cggagaaagc tgggtctcgc ccttcggcgt
    70081 aatcttcccg acgaggatgt ccccgggctt gacggacacc cccatcctca cgatgccgct
    70141 gtcgtccaga ttccgcaggg cctcctcgcc gacgttgggg atgtctcggg tgatctcctc
    70201 cttgcccagt ttggtgtccc gggccatggt ctcgaattcc tcgatgtgaa tggaggtata
    70261 gacgtcttca cgcacgaccc gttcactgac caggatggag tcttcgtagt tgtagccgcc
    70321 ccaggacatg aaagcgacca tgacgttgcg tcccaaggcg agctcgccgg tgtcggtggc
    70381 gggcccgtcg gcgatgatct gtccctgccg gacacgatcc cccagattga cgatcggctt
    70441 ctggttgaag cacgtgtcct ggttcgaccg ctggtacttg atgagattgt agatgtccac
    70501 ccctgtgtcg aggtcgtcct tttccggtcc ctcggacttg atgacgatcc gtgacgcatc
    70561 cacgctttcg acgacgcccg cgcgtttggc cgccaccacc gccccggaat ccctggcgac
    70621 gatcgcctcc ataccggtac ccaccagggg aaccttcggc ttcatcaacg ggactgcctg
    70681 gcgttgcatg ttggatccca tgagcgcccg gttggcgtca tcgtgttcga ggaagggaat
    70741 cagcgccgcg gcgacgctca ccaactggtt cggggacacg tccatcatgt tgacttcggt
    70801 gggaacgacc gtctggaagt cgcctccctt tcgggcggaa atcatttcac ccgtaaaccg
    70861 tccgttctgg tccaggggcc agtccgcctg ggcgatgatg tgatcttcct cctggatcgc
    70921 cgtcagataa cggacatcat caaggacctt gccgtcttcc accaggcggt agggcgtctc
    70981 gatgaagccg aattcattca cccgggcgaa ggtcgacagg gatacaatca gtccgatatt
    71041 cggaccttcc ggcgtttcca ccgggcagat ccgcccgtaa tgggtgggat ggacgtcccg
    71101 cacctcgaac ccggcacgtt cccgggtcag cccccccggc cccagggcgg aaagtcgcct
    71161 tttgtgggtg atttccgaca gggggttggt ctggtccatg aactgggaca actggctgct
    71221 gccgaagaac tcgttgacga ccgcggaaac cggcttggcg ttgaccaggt catggggcat
    71281 catggtttcg atgtcctgca ggctcatctt ctccttgatg gcccgctcca tcctgaccag
    71341 accgatgcgg tactgattct cgatcagttc tcccaccgaa cggacgcggc ggttgcccag
    71401 atgatcgata tcgtccacgc tgcattcggc gctgccgttc ttcagatcga tcaggtattt
    71461 caccgccgcc aggatatcct cgttgcgcag gacggtcagg tctgtcgcca catcgaggcg
    71521 gagcttgtaa ttcatttttg cccgcccgac atcggagagg tcataggtgt cgggatcgaa
    71581 aaagaggctg tggaagaact tgtgagccgt ctccggggtc gggggattgc ttggccggag
    71641 cctccggtag atttcgatga tggcatcctc ggccgattcg atccggtcca tggccaaggt
    71701 atcccgaatg gacgcgttgg ccatgtcttc gtccagccgg atgaagctca gttcggacac
    71761 gcccctcgcc aggaccgttt ccagtccctc gaggctcagg gcctcgttgc agtgcaacag
    71821 ggtctcgccc gtggccgcat cccggacatc gctcgccagg atcttcccga cgagatccgc
    71881 ctggttgacc tcgatcttct ggatcttcag ctcccccatg cgcttcagca gggcgcgggt
    71941 cagcttgcgg cctttcttaa cgacgatttc ccccgtcttg gcgtcgacga catcatcggg
    72001 ggccttttcg ccgatcaggg aatcttccac cagaatctgg gggcgatcct ggtccaccgt
    72061 gattttctcc acgcaataga agtaattgag gatctgctcc gtagagttcc ccatcgcctt
    72121 aaggaggatg gtcaccggca tcttgcggcg acgatcaatg cgcacgtaga ggaggtcctt
    72181 ggaatcgaat tccaagtcca gccacgatcc ccggatggga atgacccggg ctgaatagat
    72241 cagcttgccg ctggccaggg ttttcccctt gtcatgatca aagaagatgc cgggcgaccg
    72301 gtgcagctga ctgacgatga cccgttcggt cccgttgacg atgaaggtcc cgttttccgt
    72361 catcagggga atttcgccga agtagatctc ctgttccttg atgtcccgga tcggccgcgg
    72421 ttccagcgtt cggtccgcgt cgaacaccac gaggcgcgcc acgattttca gcggtgccgc
    72481 gtaggtcatg cccttctgga tgcactcctt gacatcatag cggaccgtcc cgatcttgta
    72541 actgacgaat tccagggagc actttccgct gaaatccgtg atggggaaga cgctcctgaa
    72601 ggcgccctga agaccgaaat tccccctttt ctccagagtg acgtccatct gcagaaattt
    72661 ctcgtacgag cgcacctgga tgtcgatcag gttgggaata tccagtatcc tcttgatttg
    72721 accgaagttt tttctcaact cgcccatagg catccttcac gattttttga atcccgtcgc
    72781 cccggcggcg catcccgaac acgtccggca tggccgccgc cgcgacctcc gcgctttcac
    72841 gggccctcgc ggcaggctga atccgctgcc gggagggagg atccagctta tttaatttcg
    72901 acggtaccgc cgactttttc gatcttggcc ttgatgtccg ccgcctcgtc cttggaaacg
    72961 gcttccttca ccggcttggg cacgccttcg accagatcct tcgcttcctt gagacccagg
    73021 ccggtaatgg cccggacttc cttgatgacc tggatcttct ggtccccgaa accggtgagg
    73081 atcacatcaa actccgtctt ctcttcggac gccgctgcag cggtggccgg tccggcggct
    73141 gcggccgcga ctgagacggg ggccgccgcc gatacgccga agcgcgtttc cagttccttt
    73201 acgagctcgg aaagctcgag caccgtcatc ttctcgatga attgaatgac atcttccttc
    73261 gtgatctgtt ctgccatctt cctcattcct tcctgtctgt ggtttatggg tttattgcgc
    73321 cgcttcctgt ttagcctgat aggcaccgag aacctgcacc agccccctcg gcaccgcgct
    73381 caggacgttc accagcgagg tctgtacgcc aatcatgacg gacatgagct tcgccaggag
    73441 gatttccctg ctcggcagct ccgccaggac ggcgagctgt tccggcgtga tgaccttccc
    73501 attcagaaca ccggccttga tttccagttc cgcgttcttc ttggcaaaat cgaccaggac
    73561 cttcgtcgcc tccacaacat ccccgtcatt cagagccagg gccaagggac ccttgagctg
    73621 ttcagaaaac acctgcatat ccgtatcctt ggacgcgata cgcaacaggg tgttcttcac
    73681 aaccttcaaa tccgtccccg tctttctcag ggcattccgc aaagcggtca gtttttccac
    73741 gttcatgccg ctgtaatcgc tcaggacggc cagcttgaaa gactggagct tttcccgaag
    73801 ctcgacgacg acttgctcct tctgctctcg atccaacctt caacctcctt tcagtttctg
    73861 aatttccgat gctgaaaccg cgattgtcgc cgtttccggt caccgcgcaa acgtcagaaa
    73921 actgatccac atacagggga aaaatcgaaa atccgaccgt aaataccgga tgtccaacgg
    73981 acacccgtgg tctcggcagg ccgaaacgct cgtttaaacc ctgtacctgc tgtctctgac
    74041 gtctgattcg gtctttttat atccttatct caaatcgttc tgacatcctg agggtctact
    74101 ttcaccccgg gacccatggt ggtggacagt gaaatgcttt tcaggtaagt ccctttgctg
    74161 gaggccggct tcaacttgac gatctgttcg atgacggcct gaacgttctc aagcagtttg
    74221 tcgaccccga aggatacctt gccgacgggg ctgtggacaa tgcccgcctt ctcgacgcga
    74281 aattcaacct tgccggcctt gagttcctga acggcttttc ccacgtcgaa cgtgacggtc
    74341 ccgaccttgg ggttgggcat caagcctctc ggacccagga ttttccccag ttttccgacg
    74401 ctccccatca tatcgggagt ggcgatgacg cggtcgaact ccagccagcc tcccttgatc
    74461 ttttcgatga ggtcttccgc cccggcaaaa tccgctccag cgtcctgggc ctccttttcc
    74521 ttttctccct tggcaaatac gagtacccgg atcgtcttcc ccaacccgtt cggcaacacg
    74581 acgctgcccc ggaccatctg atccgcgtgg gccggattca cccccagccg gatcgcggtg
    74641 tcgacggttt catcgaactt cgcctgagcc gatttaacca cctgttccac cgcctcccgc
    74701 aacgggtagc gctgaccgga cactatggct ttctttgcct ccagatattt cttaccccga
    74761 cttgacatga ctgaacctct acgtttgttt catcagataa tttcgatccc catggacttg
    74821 gccgtccccg agacgatcct gacggccccc tccatatcca cggcattcag atcgttgtat
    74881 ttcaactcgg caatctcccg aatctgcttt tccgtgacgg accccacctt atcgcgtttc
    74941 ggatccccgg cacccttggc gatctttgcg gcctgcttga gcagaacgga cgcggggggg
    75001 gttttggtga cgaacgtgaa ggagcggtcc gcgtaaaccg tgatcacgac gggaatcacc
    75061 gtcccctcct ggttctgcgt catagcgttg tacgccttgc aaaactccat gatgttcacg
    75121 ccatgctgcc ccagggccgg tccgatcgga ggcgacggcg tcgccttgcc ggccttgatc
    75181 tgcagtttga tattcgcgat aaccttcttt gccattggga tcacctcaat ccaggcgccg
    75241 gtcgccgggc aacgggccgg gaacgctgcc tccccctgaa ccctgatcgc ccgccgcctt
    75301 cagccgtatt gtgtcgctat gccctcaatt caggaccacc tgaataaaat ccagttccac
    75361 cggcgtggaa cgtccaaaga tgctgacaat gacgcggagc ttcccctttt cgggcttgac
    75421 ctcgtccacc atcccgtcga aattcgtaaa gggaccatca atgatccgaa cgtgatcgcc
    75481 ctcgtcgaac ttgaatttcg gcttgggctt cagcgtcccc tcgtcgatgc gggcacgcag
    75541 gttctccaca tccttgtccg gaatcggcga gggcttgtcc ttactcccga tgaaccccgt
    75601 caccttgggg gtatccttga cgatatgcca ggtttcgtcg ttcatctcca tccggacgag
    75661 gatgtaaccg gggaagaatt tccgcttcga cgtcttcttt tcgccggaga tcagttcgac
    75721 gatgtcttcc tccggaatca ggacatccga aaagaagtcc tgcatccccg ccgactcgat
    75781 gcgctcctgg agggacatct tcaccctgtt ttcgaaaccc gaatacgtat ggacgacata
    75841 ccagcgcaag gccatatcct taacccaaca cgagtttaat gatcttggta aggccgaaat
    75901 ccaccagccc caaaaacagc gatacgacag ccgacacgag aatcaccacc gaggtcgaag
    75961 cgagggtctg cttcggcgtc ggccatgtca cctttttgag ttcaacccgc gcctctttca
    76021 aaaacttacc gacctgcgcg atgatcaact tcattttgtc cattcgatgg cctctgttcc
    76081 gagcgtcccc tgaaaaagtg gcaggccagg agggactcga acccccagca tccggatttg
    76141 gagtccggcg ct
//
LOCUS       DAOOLJ010000006        75003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_189_length_75003_cov_18.600163, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000006 DAOOLJ010000000
VERSION     DAOOLJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 75003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75003
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_75003_cov_18.600163"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLW16_02090"
     CDS             <1..494
                     /locus_tag="PLW16_02090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPB69727.1"
                     /translation="PDDDVFDVIEMILAEGKTSRLHRRLVQELELATEVSAFSAPGHR
                     YPNLFIVAATPRSPHSPGEVEQAVERELEHLMTEPVTERELQQILHRLEYEEVVRMSS
                     NNGLASVLTEYEAVTGNWRCLIEHRRRVAAVTPADVMRVAKTFFTRGNRTVGTIVRKE
                     AAP"
     gene            491..1921
                     /locus_tag="PLW16_02095"
     CDS             491..1921
                     /locus_tag="PLW16_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB69728.1"
                     /translation="MKAALRRGLALVVLGVLFAATPLGAAAPVDPRTLTFPPLTYDIP
                     SPERFVLDNGMTVLLMTDRALPLIQITAYVGVGAVYEPADKCGLAELTGAMIRRGGFA
                     GKTPEALDAELEFMASEVESAIGDDLGTVSLRTLRKNFEATLGLFAGVMRSPAFDALR
                     FDQTVKEALETLRRQNDDPQELADRELLRAIYRGHPLGRTPTPATLRAIGREDLAAFH
                     RRYYRPNNVLLAVSGDIDRAELEDRLRSAFDGWEPAPVDFPTVPWPDMTLRPAMLYVK
                     KELAQSVIRMGHLGITKDNPDLYALRVMDFILGGGGLNSRLMGEIRANQGLAYNVSGY
                     FEIGRRFPGPFLAETETKAASTGRAIALMLDIMDGITKVPVTEAELTLAKESIVNAFI
                     FAFANAERVVTQRARIVYYGYPERYLEDYRANIAKVTRADVLRVARRYLHPDKLIKVV
                     VGDAARFDRPLSAFGEVREIRPDAWN"
     gene            1964..3361
                     /locus_tag="PLW16_02100"
     CDS             1964..3361
                     /locus_tag="PLW16_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB69729.1"
                     /translation="MTNRSLPDAFFKPGTVAVVGASKKAMFSSGIPDTLVKYGYRDRV
                     YLVNPRETEINGMPVYARLADIPAPVDLAIVVVPATQAPIVVRECVKLGIPAVVLESA
                     GFGETGPEGARLEEELRRLLRGARTRVIGPNCLGVINPHESFATTPVDLKALRPGNIG
                     VIAQSGTFGNILADWAPTQDLYFSKLVTIGNRLDVDETDCLLYFAEDAQTDVIVLYLE
                     GVKDGRRFYEATREVSRRKPVLVYKGGRSPAGRMAAASHTGSLTGEDELYDAIFRQSG
                     VTRAASFQELFEMARVFSREPLLTGPRICVVTASGSLGVMMADACMTGGLELPGLSPE
                     TVSVLRELAPAWMNVKNPLDVGPSGIFAAAMKAVAADPRIDGIIAVPVFPEEILRRFA
                     AMGIDTRSMNGDPREWRNLAPDKPFLMFTVGGPSWLESIRKTYGPAVSLVGSPEIAAR
                     SLAALYRYSSFRARG"
     gene            complement(3491..4060)
                     /locus_tag="PLW16_02105"
     CDS             complement(3491..4060)
                     /locus_tag="PLW16_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69730.1"
                     /translation="MKTKAVCLSLLFLLITAFELTPFPSFAQDGAEQRRRLRGDAAQH
                     MVDEATRAVDQANRDRRRIRPNVAPDRAARQTDTGRYGGDDINPAQTSDDPTYGYTRE
                     NPVKLGGDSGSGAAYSHVYLKQLRDKNRRPFKYYRVGNVGAGPDGHITDQYKLTDSDG
                     SEFTLFIDMYHPEKNPLDCLAPKGMFMAP"
     gene            4253..7159
                     /locus_tag="PLW16_02110"
     CDS             4253..7159
                     /locus_tag="PLW16_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPB69731.1"
                     /translation="MLLLSMPGNVCGSDAVTADLLQARYDRINRLLKDGRLDDALQQA
                     LRTLDMARGLYGQESAGTAPAIDRVAAVYRAMGRFADAETNLIAALDLVRRGPDLPPE
                     YTVSALNNLAGLYREMGRYGDAEALYLRAVDMQKSLRDESGEGEAALLSNLAVLYMNL
                     ARYADAEPLLKRVLAMAEKKAEPDRLSQRMKISMDGRRLEWGRRMLNLANLYFEMKRH
                     EDAESLTMRALGIFQPILGEEHPEVARVKGDLGVLYSATGQYARAEKLLTEAINSAST
                     AYGDKPQPVVAEYYVSLAQLYQEMGNYRDALKVAGHALPILQNTLGGSHPKTLRCLNT
                     IAVIHIYRGEYEQAESVLRQVLETARASLGSRHESLAVYLSNLGNVLAARGRHREANR
                     MFASAIGINEQKREDAFLILSEQQKLNYVSSMLHQIKLYLSHLLQHGVSDPEALGEAM
                     TVWLRWKGAVQEAQGRYGDALYRSDDPVLRRKFDELVRVRRELGGIQLFGGQAARQRG
                     AAETASRLEKRKQALEAELSGLSRDYAFERRIGKVDIARLTELLPPDAVYIDFAEIAM
                     TDFSRQMPGRMHYAAFVVASGGKVPVRLLDIADRDALDAVIGSYLKEMRKKAAGRPDH
                     DPTRLERDAVQLYRMLLNPLLPGLNGRTRLYVSPDGLLNLIPLEVLKSESGRYAVEDY
                     RINYVASGRDLARFDTVDPPVGRVVILADPDFDLKRPGQGDTQTPAVEAAEDGRRPAV
                     GIFSGVHFDRLPDTKQEAEAIGTLLKASGYRVDLYEGREAVEENLFAVRHPRILHIAT
                     HGYFLNGNAAASAATRGVRILLQEPVLTNLNDPVQNPMLRSGVALAGANASIRVGTDH
                     GLVSAERVLGLNLRGTDLVTLSACETGTGDIRAGEGVFGLKRAFLMSGAKTLVMSLWN
                     VPSAETTTLMTEFYTRMSRGALKASALREAKLKMMKQNPHPFYWGAFIMVGKPE"
     gene            complement(7303..8352)
                     /locus_tag="PLW16_02115"
     CDS             complement(7303..8352)
                     /locus_tag="PLW16_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB69732.1"
                     /translation="MKKKPARTTAQAEAKKINLALQGGGAHGAFTWGVLDRLLDDERI
                     NIEGIVGTSAGAVNASILAYGLIAGGRTKAQELLRRFWEFNSKNGRCSPFQPTFFDKL
                     YGSKGSMEFSPWWQTFDVLSRMFSPYQWNPNDTNVFRDILEELIDFPVLQRAHTVRLF
                     ICATNVLTGRLKVFENQEITPKMILASACLPYLYQAPEIDGEYYWDGGYMGNPPIFPV
                     IYNTDCQDVLIVQINPINIHEVPRSANAIFDRINTLSFNSSLMREMRAIHFVTSLIEK
                     GELDPAKYKHTFIHTIDAEEQMSKLTASSKMNLDMEFLKYLFETGREKAGEFLANHYD
                     DIGRKSSTDLAAKFF"
     gene            complement(8455..10434)
                     /locus_tag="PLW16_02120"
     CDS             complement(8455..10434)
                     /locus_tag="PLW16_02120"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB69733.1"
                     /translation="MPDIQHPLHRDLTAGMRRGKIVSIEEAADLIQDNDVIGFNGIIG
                     IGCPDEIICALEKKFLETGHPRNLTFFYASTLGDGNEMGLQHLAHEGLVKKVIGGHFG
                     KTPKLTQLALDGKIEGYNLPQGVLSVMIRDIAGGRPRTITKVGLGTFVDPRFGGGKLN
                     ANTKEDLVEVIAFDGQEYLAYKPPRLNIAIIKATTADLEGNIGFEKEPLYLDALSLAT
                     AAKNCGGFVLVQVEGLAERGALPMRSVIIPGALVDCVAVVTNAERHRQTAATLYNPAY
                     AHQYRIPVTLVPPIPLNERKIIARRAAFELKPNSIVNLGIGVPESISSVAAEEGIQEY
                     LTLTSEPGIIGGVPAGGGDFGAAVNVDALIAMSHQFDFYDGGGLDVAFLGLAEADEAG
                     NLNVSRFGPRIAGCGGFIDISQNARKVVFMGTFTAGGLKIDLRDGEMKIVQEGKVHKF
                     VKRVEEVTFSGAVAAADRKYVLYVTERCVFRLTPEGLELIEVAPGIDVERDIVGQMDF
                     KPVIRQAPRLMDHRLFKPVPMELKKDLLDIPIVNRLRYQPEDNLFFVNFEGLAIRSRE
                     QIDEIRAAVSAVMEPLGKKVRTIVNYDNFDLSPELVDYYTEMVSYVVGKYYDKVSRYS
                     TGAFLRMKLGDKLNERGMSPRIYETPEEAREEIDV"
     gene            complement(10600..11067)
                     /locus_tag="PLW16_02125"
     CDS             complement(10600..11067)
                     /locus_tag="PLW16_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPB69734.1"
                     /translation="MTEKLHLKKYTNRRLYDTERSAYVTLDDVLQVIRQGRRVEVVDA
                     KTGEDVTAFTLTQIILEESRKKNSLLPVPLLHLIIQYGETVLGEFFDHHLEQTLKSYL
                     SYKAAADEQFRKWLSLGMDFSKMTQTSIASLPAFQSFFDLFHDTDKPGGKGEA"
     gene            11352..11645
                     /locus_tag="PLW16_02130"
     CDS             11352..11645
                     /locus_tag="PLW16_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69735.1"
                     /translation="MEQEKLTKQMIEFHKTTFDNTFNAVEILQEQTEKMVGMFLDQAP
                     WFPAEGKQAVNEWLKTYKEGRETFKGSVNESYKKVEEYLGALEKKAPRTKAGK"
     gene            11719..11997
                     /locus_tag="PLW16_02135"
     CDS             11719..11997
                     /locus_tag="PLW16_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69736.1"
                     /translation="MEPKQMAKQMIQFNKAAFDNTFNAMTVLQDQAEKMMNSLIDQSA
                     WMPAEGKKALSDWVKAYKRGREDFKAAMDDNYKKVDAFFAGLEKGKSA"
     gene            12124..12609
                     /locus_tag="PLW16_02140"
     CDS             12124..12609
                     /locus_tag="PLW16_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69737.1"
                     /translation="MDARFYEFWGRFFSSLAQGRNPWEDWIPGRTPVAGGADAFSEFF
                     RGMDGGERRPEGDLPGGELWQEAAEAFQNSLRDFLALFDVVSREDYRALQDECAALKE
                     TVASQEKTIAGLKRRRGRKTGASPEETVKRLREVIRRQNEEFESLMKAAAGLFEGKPT
                     S"
     gene            complement(12667..13092)
                     /locus_tag="PLW16_02145"
     CDS             complement(12667..13092)
                     /locus_tag="PLW16_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPB69738.1"
                     /translation="MNGKSIDRIKVGDAAEFSKTVTETDIYLYAGITGDLNPAHINEA
                     YAKNTFFKTRIAHGMFTAGFISNLLGMQLPGPGTIYLKQSLSFLAPVRFGDTVTARAE
                     VVEVMAEKNRVRLKTTCTNQDGTVVLDGEALVSPPKAKP"
     gene            complement(13122..14168)
                     /locus_tag="PLW16_02150"
     CDS             complement(13122..14168)
                     /locus_tag="PLW16_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB69739.1"
                     /translation="MTKQDNYFRTIVKIGRALGTTLERDKILQLIVQGVLEAMGGKAA
                     CLFLMDEEKGTYVPVAQTGLSKNYVHAGLAHVKKEIPLLLKKGYLHFRDAASDPVVEN
                     REMKRAEGIASILVVPVKVKGRFIGTLSLYTAKVRNFSAGEIEFLTVLAEQGGVAVET
                     ARLVERLRENTRIFLDLAASINSSLDIKDILQTLSCEVSQAIGVKAASIRLLDEESNT
                     LKLVASCGLSETYLNKGPVSAEKSIAEALKGKPVVVRNASTDKGVQYKKEKKAEGIVT
                     ILSVPIKSKERVIGVLRLYSAVAREFTEDEVLLVTALAYQGGLAIQNAGLYLMLKSDM
                     KELKDEIWSHRCWF"
     gene            complement(14234..15469)
                     /gene="phaC"
                     /locus_tag="PLW16_02155"
     CDS             complement(14234..15469)
                     /gene="phaC"
                     /locus_tag="PLW16_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HPB69740.1"
                     /translation="MDQPKIPLDLILASLAEDTEKAQARVTKVSEVLLGELSTEIAPT
                     PYDIVYEEDRVRLKHYRLEGEARLKTPLLVVYALINRETMLDLQPDRSVVKNFLRDGI
                     DLYLIDWGYPSRKDRFLTIDDHVNGYLDHAMDFIRRKHNLPRINLMGICMGGSFCVMY
                     AALHPEKVKNLITTVTPTHFDTDTGLLHIWMKALDADRMVDTFGNIPADLMNLGFLLL
                     NPARLMIDKYVGFLENADNKSFVENFVRMEKWIFDSPDVPGETFRQFINDCYKKNLLI
                     QSKMKLGGKRVDLKKITMPLLNFYGMYDHLVPPAACEQLTRHVGSRDTEDVNLDTGHI
                     GIYVSSKSQREFAPKIARWLMERDGPDARPASKGAAIVKFTPGKAARGKGRPAPARPP
                     KKKKAERGRARPAQRAANK"
     gene            complement(15503..16396)
                     /locus_tag="PLW16_02160"
     CDS             complement(15503..16396)
                     /locus_tag="PLW16_02160"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HPB69741.1"
                     /translation="MDETQQGQAPDGLFPPDWIKAGADLWTSLLKMVPPPTEAAAGPS
                     TQKPPRKRSLESMEAALRMWQSLSTAMSDPAVAGAASKSLDALPDLFLKIAEASWDTC
                     FQIQKGQIEKAGKIGRKVEAYHFENLDQEFFKTLKDIYEKEVRQFFYIPQLGLTRFYQ
                     ERMNLLLDKLALLKATGAEFFSLLNLPFEQTTKVMQQEIEKLAQQGKVPKQAREYYNL
                     WVKILEGHFMTLFKSPEYSQALGELFNRLSEFIIAKNEVVQDLLQLLPVPTHKEVDAL
                     YRDLHQLKKRVRELERQLNPD"
     gene            complement(16566..17438)
                     /gene="lipA"
                     /locus_tag="PLW16_02165"
     CDS             complement(16566..17438)
                     /gene="lipA"
                     /locus_tag="PLW16_02165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPB69742.1"
                     /translation="MNGKIAVDPLRKPPWLKVRLPGGEADRRIARTLAELRLHSVCQE
                     ALCPNRGECFRAGTATFLILGDTCTRGCRYCHIRQGTPTPVDADEPERLVEAVRALGL
                     RYVVITSVTRDDLPDGGAEHFARCIHLLRTRLPDCRIEVLIPDLQGSREALERIIDAG
                     PEVINHNIEVARSLFPNLRPQGDYKRSLRLLAALHPHRDIVSKSGFMIGLGETRDAIV
                     ALLHDLAVVRCQRVTIGQYQQPTRRHWPVARYYPPAEFAEFRKIALGLGFRHVEAGPL
                     VRSSYHAGIFSLDG"
     gene            complement(17584..19419)
                     /locus_tag="PLW16_02170"
     CDS             complement(17584..19419)
                     /locus_tag="PLW16_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB69743.1"
                     /translation="MVKNILSLDNKELVEKLWAADPEIRRILAGSKHVEEARHILFDH
                     FNQLNRSMFNMKPDTPYAGMNSIEKRNAKECLRVFSNTIRTENEHLTGASPLTLLYDL
                     ARNRPGALERVGKAFLCEYLAFFKGITGKAGKQPKIPALLRTADGRSAAQDRSAATVR
                     SRQLDAYSAIMRRYRRRYRTGFDAALIRQRRKLKAKILAHFGAAEADWRDPRWHLKHI
                     LTDLDTISRLVRLDDDEAAGLAMAAEKGIPVEITPYYLSLFNASGRTDEDRQIRAQVL
                     PSVRHCASVAANREQGVDMDFMQERSTSPVDGITRRYPKVVILKPFNACPQICVYCQR
                     NWEVKQIDAPAMLPPEALREAVAWIRDNDHISEVLVTGGDPLCLDNRRLEWIVGQLAC
                     ISHIERIRISTRVPVTLPFRIDDGFLEILRKYHEWGRREICVSTHFEHAVELTPEALE
                     AVKKIKALGVNVYNQHVFTYYNSRRFELCLLRRMLKLSGIDPYYTFNTMGKEETVDFR
                     VPLARIEQERKEEARLLPGVVRTDEPVFNVPRLGKSNLRAWQDHELIMVLPDGRRVYR
                     FYTWEARITTNFDYLYTDVSIYDYLKRLAADGENTSDYKSIWYYF"
     gene            complement(19606..21087)
                     /gene="gcvPB"
                     /locus_tag="PLW16_02175"
     CDS             complement(19606..21087)
                     /gene="gcvPB"
                     /locus_tag="PLW16_02175"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPB69744.1"
                     /translation="MEKHPSHPNVELIFEKSRPGRRAGAIPPCDVPGTADEPRIEAHL
                     LRDDVDLPEVAEIDLVRHYTALSRRNFGVDLGFYPLGSCTMKYNPKVNDMAAALPGFA
                     DLHPGVPAAFAQGSLQLMDGLRRDLCEIFGMADFTLQPAAGAHGELTGVMMIRKYFEK
                     REEKRTQILIPDTAHGTNPASGALCGYEVVQVPSNDAGGVDLDALDAAMTENTAGLML
                     TNPNTLGLFERNIAAVARIIHDRGGLLYGDGANANAFLGKVRPGEIGFDVVQLNLHKT
                     FSTPHGCGGPGSGPVGVGAALEPFLPVPRIVRTDAGYAWSEAYPDTIGRVRAFYGNFA
                     VMVRACAYLRSLGAAGLRQTAEDAVLAANYIQARLKTFYDLPYNRPCMHECVFSGRRQ
                     VRESGVHTTDIAKRLLDYGVHPPTIYFPLIVPEAIMIEPTETESLETLDAFCDAMIAI
                     ATEAKDEPEIVKSAPHTTPVKRLDEVLAARRPDVCWKRPSGPA"
     gene            complement(21304..22632)
                     /gene="gcvPA"
                     /locus_tag="PLW16_02180"
     CDS             complement(21304..22632)
                     /gene="gcvPA"
                     /locus_tag="PLW16_02180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPB69745.1"
                     /translation="MDYSPHTAEDRKTMMDAVGIASIEDLFGDIPERWRLSRPLDLPP
                     ALSEQETFTHLKALSERNRLPDITLTGAGAYRHYIPAVVGHLSQRSEFSTAYTPYQAE
                     ISQGILQAVYEYQTMIARLTGMQVANASMYDGATATAEAAVLSARTLGRTGVVVARSV
                     HPAYRQVLRTYAWANGLSVEEAPFTGDGRLDLEALAAAVRESTAAVIVQSPNYFGVVE
                     DLAAVAPLVHAAGAYLIHCFTEATSLGLLKPAGGLGADLCVGEGQAFGNPVSYGGPYL
                     GIFAATDTFLRKIPGRLAGATVDRDGRRAFVLTLQTREQHIRREKATSNICSNEALCA
                     LAAAVYLVCLGKNLKPLAELNVAKAGYLRRRLLDLPGWAPAFSGPVYNEFVLRCPDPE
                     GANARWAAAGISGGVNLAGDYPELPGALLLCATELLTRADLDRAVDVLRP"
     gene            complement(22652..23050)
                     /gene="gcvH"
                     /locus_tag="PLW16_02185"
     CDS             complement(22652..23050)
                     /gene="gcvH"
                     /locus_tag="PLW16_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPB69746.1"
                     /translation="MSKPNPTDRKYSQEHEWALPHGDGTAAIGITDHAQELLTDIVFV
                     ELPALGRQVRQGEAVAVVESVKSVSDVFAPLSGEVVAVNRALEDRPELINQDACGEGW
                     IAKLKLSDPREYDGLMDAAAYDAFIRDEAH"
     gene            complement(23093..24187)
                     /gene="gcvT"
                     /locus_tag="PLW16_02190"
     CDS             complement(23093..24187)
                     /gene="gcvT"
                     /locus_tag="PLW16_02190"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPB69747.1"
                     /translation="MNQTPLYDRHVHAGARMIDFGGWAMPVQYAGVIEEHRATRKSAG
                     LFDICHMGEIEVRGAAALAFLQRVMSRDLGDLETGRLKLGVLTSETGGILDDLTVYRL
                     ESDRYGLVTNAGTRLKDAAWLKTVKAAEGFRKVEIVDRTDEIGKLDLQGPVAQAILQP
                     LTPAALDGLAYYRFTHTRVADRPALVSRSGYTGEDGFEIYAASDEIGAIWDALLESGA
                     ARNLKPVGLGARDTLRLEAGMLLYGHDMDETTSPHEVTYGWLVDPAKDFIGSDALRRQ
                     KAAGVERKLVGFEMVGRGIAREGHPVWRNGEAIGAVTSGTFSPTLSRAIGLAMVSATA
                     KDPGTEICIQIRDHLVPAVVVKLPFYRRPR"
     gene            complement(24329..24652)
                     /locus_tag="PLW16_02195"
     CDS             complement(24329..24652)
                     /locus_tag="PLW16_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69748.1"
                     /translation="MVKIQCPQCGKSFMWTDGMPIRGRCPTDDCRWTYDVGEQIKKSA
                     EAKTSSPKDELRCPNCGAPIASHLTVCQSCDGVVVGGKALRKSNIFFGVVVILILLSL
                     LYRYY"
     gene            24859..25593
                     /locus_tag="PLW16_02200"
     CDS             24859..25593
                     /locus_tag="PLW16_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPB69749.1"
                     /translation="MIRFQAVHKWFKKLHVLNDVNLHVKQGEVVVVCGPSGSGKSTLI
                     RTINKLEPIQKGKLFVDGQDLSDRKTDINKLRAEIGFVFQQFNLYPHLSVIKNITLAP
                     IKIRKLARKEAEEQATALLERVGLAEKRDAYPAQLSGGQQQRVAIARGLAMKPKIMLF
                     DEPTSALDPEMIGEVLEVMQGLAHTGMTLIVVTHEMGFAREVADRVTFMDYGVILEEA
                     TPDEFFKNPQHERAQLFLKEILSPMH"
     gene            25691..26515
                     /locus_tag="PLW16_02205"
     CDS             25691..26515
                     /locus_tag="PLW16_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB69750.1"
                     /translation="MMKRLGMWGLALVFACSLGGTALAADTLADVKSKGVMVCGVKDS
                     TPGFGYIDEQTRQIIGYDVDFCKAIAKKLGVNLELRPVTSATRMPQLIAGNIDIIAAT
                     MTKNAERAKQIDFSYMYFFTGQKFLVKKGTVKKLADLEGKRIGTAKGSTSEQNAAKAL
                     PKATILSFDDYPQAFLALEQGKVAAVTTDESILANLLAKAPNKRKYEIPNIQISDEPY
                     GLGMRKGDVNFVNAVNKALLDMEKKGEAKKIFSKWFGSKGKSGIVLKRTFTITPER"
     gene            26534..27241
                     /locus_tag="PLW16_02210"
     CDS             26534..27241
                     /locus_tag="PLW16_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB69751.1"
                     /translation="MFNYQFDWAIVTSGQYFEWILSGVKVTIQLSLVSIALAFILGLL
                     IAVMRMSQNAPVRWLAMGYLEFFRNTPLLVQIFFWYFGSDKILPQAARDWLYTMNFEF
                     AAAVIALTIYTSAFIAEDIRSGVRSIPKEQMEAARSSGFSYLRSMLYIILPQAVRITI
                     PPLINQFLNLAKNSSLAMTIGVAELTYQARQVESYTFKGFEAFTAATLVYLVISLVIT
                     GLMTLYSKKVLSPIGMR"
     gene            27245..27946
                     /locus_tag="PLW16_02215"
     CDS             27245..27946
                     /locus_tag="PLW16_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB69752.1"
                     /translation="METGLDFTVIWTNITHFMIGRYPSGPMGGVLLTLYLAAVSLVLS
                     FFGGLILGLMCVSRNPLVRYPTLAVVNTIRGIPLLLVIFWMYFLLPTFLGYRTPESWT
                     VIAALTIFTSAYMSQIVQAGIEGIPSGQMEAALSTGLHPRQAMLFIVLPQALRNMIPS
                     FVNQFVSMIKDTSLAYIVGVSELTHVATQINNRTMIYPTEIFLFIAVIYFIICFAFTS
                     LSRWLEKRLAWRKAI"
     gene            complement(27996..28559)
                     /gene="def"
                     /locus_tag="PLW16_02220"
     CDS             complement(27996..28559)
                     /gene="def"
                     /locus_tag="PLW16_02220"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB69753.1"
                     /translation="MTEGKILTLWSDDHWNEAEAKRLRESCEELPIPLNRAAREDIRA
                     LMDAFLERDDALGLAAPQIGIGRRVIIFKNKHFDEKGRPASDKDYDVLVNPRITQTRG
                     EPVRGAEGCLSCPEIQVEIERHPEIKVRAYDREGRKINKRYTGFGARVVQHEMDHLEG
                     ILIVDHEGAVLVPRKKRAFFESVLSKK"
     gene            28816..30078
                     /locus_tag="PLW16_02225"
     CDS             28816..30078
                     /locus_tag="PLW16_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HPB69754.1"
                     /translation="MTLPFVPSLITAVARREFSRAMHFEPGRPVDFPPGDRSAPRLLY
                     LHVPFCEQLCPYCSFNRVVFEPELCRAYFRALRREIRLYAERGYDFGGVYVGGGTPTV
                     LPGELGETLDLVRASFGIREISVETNPNHLTDETLALLKDAGVNRLSVGVQSFQDPLL
                     RQMERYAKYGSGEAITRRLGQIAGRFDTLNADMIFNFPSQTPEMLAEDLAVIRSLGVD
                     QVTWYPLMVSDSTRRKVSRTLGRVRYSRERRYYGEIVKGLLPMYRLSSAWCFSRQPSL
                     IDEYIVDYAEYAGLGSGSIGYLDGTCYANTFRIPDYIAQVERGEIPLLAARAFSRRDR
                     IRYDFLMQLFGMTLDIRGLKKKYGPAIWRHLCFPLLAFGAAGGLRYRAPVVRLTATGR
                     YLWVIMMREFFIAVNNFRDDCRNGDGPV"
     gene            30202..31689
                     /gene="selA"
                     /locus_tag="PLW16_02230"
     CDS             30202..31689
                     /gene="selA"
                     /locus_tag="PLW16_02230"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPB69755.1"
                     /translation="MDERKKTLLRELPKIDALMLLLEAKGATALAPRELILQTCRAVV
                     ADLRAEIVGVEGPGKTSSGKRGGDLRVGPESLRLPTPEEVAARVAARLEDLHRYRLRR
                     VINATGVILHTNLGRAPLCPEAVDRIVAVSRGYSNLEFDLERGERGLRYDHVRDRLCA
                     LTGAEDALVVNNNAAAVLLVLNTLAFGREAIVSRGELIEIGGEFRIPDVMEKSGGRLR
                     EVGTTNRTRLADYERALGPETALILKVHTSNFRIVGFTEEADLGELVALGKRSGVPVL
                     DDLGSGCLIGLEHYGLMREPTVQDVLATGVDVVTFSGDKLLGGPQAGIILGGREILGR
                     VKKNPLNRALRIDKLTLAALEATLIHYLDPEAARERLQVLKALTEPLPDVKKRAHRLL
                     RLLRRAGLAGLDLSLEAGVSMTGGGSLPTQEIPTSLVALRPRSLSAARLEEGLRRLPT
                     PVVARIAEDAVLLDVRTLMEEELGCIRDGLRTLFPVRPHDPGRNP"
     gene            31756..32769
                     /locus_tag="PLW16_02235"
     CDS             31756..32769
                     /locus_tag="PLW16_02235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB69756.1"
                     /translation="MDKTIETWTVSPSLDGARLDLFLTEASSLSRSQVKRLVEGGRVS
                     IDGVVAPKVSRKLKACETVVLNRPPVAVMEALPEDIPLSILYEDAHLLVLDKPAGLVV
                     HPAPGHAGGTLVNAVLRHCRDLSGIGGVLRPGIVHRLDKDTSGVLVVAKSDTAHAGLV
                     NRFKRREIGKTYLALVWGAPRENAGRIDLPVGRHPRERQRMSVRGRRGKPAVTHWRVV
                     RRYGAVSLLEVALETGRTHQIRVHLNAIGHPVVGDPVYGGAGRVRGVADPVLRFRLQA
                     MKRQALHAARLDFVHPVTGMPLSVSAPLPPDMAELTAFLEKDAGGAAPDLGNGAPRPG
                     GAR"
     gene            32859..33662
                     /gene="pgeF"
                     /locus_tag="PLW16_02240"
     CDS             32859..33662
                     /gene="pgeF"
                     /locus_tag="PLW16_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPB69757.1"
                     /translation="MFEADLLAEIPSLRHAFSTRRGGSSGGCFSSLNFSFRQGDEAEN
                     VRANWAALSRAFGIAPERFLTVHQVHGNRVWVIDGAESIPTSGDGDAREDAPRCDAIV
                     TNRPGIAIGVKTADCVPVLIADPVRRVIAAVHAGWQGTARRIVEHVCAALEARFSCRR
                     EDLRAAIGPAIGPCCYEVDDRVREAMAGLPGLERIFSPRDAPGRWRLDLALANRLQLR
                     NAGVPASRVAEANLCTACRPDLFFSHRGSGGKAGRHFSFLLMDGESGAG"
     gene            34033..35280
                     /gene="rho"
                     /locus_tag="PLW16_02245"
     CDS             34033..35280
                     /gene="rho"
                     /locus_tag="PLW16_02245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPB69758.1"
                     /translation="MNIDEIKRLPISQLTDMAKDFNVPGAGGLRRQDLIFAILQAEAE
                     KNGVIYGAGVLEILPDGFGFLRAVDYNYLPSPDDIYISPSQIRRFSLRTGDTISGEVR
                     PPKEGEKYFALLKVDTVNFESPETARDKILFDNLTPLYPQEKLNLESNPENFSTRVMD
                     LFTPIGKGQRGLIVSPPRAGKTVLLQDIAHSISANHKEVILMVLLIDERPEEVTDMQR
                     SVQGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRIADRRVFPAFDLIRSGTRKEELLTPKANLNRVWILRRLLQEMNPVDA
                     IEFIVDKVRKTQSNQEFLDSMNQ"
     gene            35409..35621
                     /gene="rpmE"
                     /locus_tag="PLW16_02250"
     CDS             35409..35621
                     /gene="rpmE"
                     /locus_tag="PLW16_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPB69759.1"
                     /translation="MKEGIHPDYQETTITCVCGNVIETRSTKKDIKIEICSQCHPFMT
                     GKQKIVDTEGRVERFKKKYAGTGGKK"
     gene            35928..36998
                     /gene="prfA"
                     /locus_tag="PLW16_02255"
     CDS             35928..36998
                     /gene="prfA"
                     /locus_tag="PLW16_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPB69760.1"
                     /translation="MFKRIKEIESRYEELERLLSDPEVIAKQAVYQRHAKEHADLREL
                     VETFRLYEKTEARIREDQELLRGPDEDIREMAKEELPILKEALDGLEERLKRLLLPKD
                     PNDERNVFLEIRAGTGGDEAGLFAGDLFRMYARFAERNRWKIEVVSSTPAGGMGGFKE
                     IIALITGAGAFSRMKYESGVHRVQRVPVTEAQGRIHTSAVTVAILPEAEEVEVQIDPN
                     DLRIDVYHSSGHGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKAKAMKVLRARL
                     LDGMVTRQNAEISEARKIMVGSGDRSERIRTYNYPQGRVSDHRIGLTLYNLEGFLDGD
                     IEEVLEALSTHYQTEALKQSAG"
     gene            37004..37846
                     /gene="prmC"
                     /locus_tag="PLW16_02260"
     CDS             37004..37846
                     /gene="prmC"
                     /locus_tag="PLW16_02260"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPB69761.1"
                     /translation="MNIRGLIDRTTRTFAREGLPTPRLDAEVLLAACLRTDRSEFYIH
                     PEREVPPDHRRRFASWVGRRRRGQPVAYITGEKEFWSLPLTVGPQVLVPRPETEILVE
                     EVLRLASSLPADSRILEVGVGSGAVSIALATELPLARIAATDISPDALALARVNARRH
                     GVDDRIALMAGDLFEPVRGPFDLVVSNPPYIPEGAYDGLPAEVRCEPRLALLAGPDGT
                     DVHRRLIADGGRYLTAGGWLCLEMGEGQGGRLTAVLEKAGYADIVRRKDYGGRERVIR
                     ARWR"
     gene            37850..39100
                     /gene="murA"
                     /locus_tag="PLW16_02265"
     CDS             37850..39100
                     /gene="murA"
                     /locus_tag="PLW16_02265"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB69762.1"
                     /translation="MDKIVIEGGRRLEGEVRISGAKNAALPILISALLTEGPNTFHNV
                     PDLVDIKTVKKLLASFGVRMEGRETLSLDATDLTRCEASYDLVRTMRASILVLGPLVA
                     RLGEARVSLPGGCAIGARPVNLHIKALQEMGAEVELRDGYVEARCPRLNGAEIYFDIS
                     TVTGTENILMAACLARGTTVLKNAAREPEIVNLAQVLTGMGARIDGAGTDEIRIEGVE
                     RLHPVDAAVIPDRIEAGTFMIAAGMTGGDIRLTGCDPTHLDALITKLRETGMSIAVGG
                     GEIRAAGGSRIKSVDITTLPYPGFPTDLQAQMMAYMTAASGLSVITETVFENRFMHVS
                     ELLRMGADIQIQGNSAVVRGTPGLRGAAVMATDLRASASLILAGLMAEGRTELLRVYH
                     IDRGYQQIEKKFSALGADIRRMRA"
     gene            39102..40406
                     /gene="hisD"
                     /locus_tag="PLW16_02270"
     CDS             39102..40406
                     /gene="hisD"
                     /locus_tag="PLW16_02270"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPB69763.1"
                     /translation="MKIRRTSERDFDAFFRDLRLRGRAFDETLLAEVKGIVADVASRG
                     DDALFALTARFDGYTPTAETVEVAPTEWSAAAAGVAAADFELIELAARRIERFHRQQR
                     IDSYCAEDEPGITWGQRILPLERIGIYAPGGLAAYPSTVLMAAIPARIAGVREILLVS
                     PAREGRLNPLIAAAARVAGVTRIFKIGGAQAIAALAYGTASIPAVDKIVGPGNAYVAA
                     AKRVVYGQCAIDMIAGPSEVLIIADGSQNPAVCAADLLSQAEHDPLASALLLTPDDAY
                     ARRVTAEVERQLPTLSRRDIAARSWSDYGAAVVTKDLAEAVDLANRFAPEHLELMVAD
                     PESLLPGIVNAGAVFMGGCTPEAVGDYLAGPNHILPTGGTARFASPLGVYDYLKRMSV
                     LSFSREALMKYGRQAARLADLEGLEGHGKSITRRMDADPENP"
     gene            40414..41208
                     /locus_tag="PLW16_02275"
     CDS             40414..41208
                     /locus_tag="PLW16_02275"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPB69764.1"
                     /translation="MKTELYYYSGTGNSLWTARLLAQALGSAAVRPISRMDGSTVTAG
                     IEAVGLVFPVHIWGPPRRVIAFANALNADPATYCFAVAVNAGQVAATLVQLKRILHAR
                     NLTLASGFGIVMPSNYIPWGGPGPEEEQRRRIAAAREKIGRIAARVGNRESRPVEKGP
                     WWQNVVLTALNRLAFPHVPAMDKGFRVDETCNGCGVCKTVCPCGNIAWEAERPVWQHR
                     CEQCLACIQWCPREAIQYGRKTPGYARYHHPEIRLRDLTRERDLRG"
     gene            41229..41966
                     /locus_tag="PLW16_02280"
     CDS             41229..41966
                     /locus_tag="PLW16_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69765.1"
                     /translation="MRKIVFAGLGGVLLCATVASATGPHFRTDAFRTSAGELVITVVG
                     HATLMMAWGGKVLHIDPVTGQADYTKMPKADLILVTHEHADHLDPAAIGLIRTQRTAV
                     LASAEAARRIPGAVVLKNGDVRTVQGVRIEAVPAYNLVHMRKSGVPFHPRGIGNGYIV
                     TFGDTRVYIAGDTENTPEMKRLKGIDIAFLPMNLPYTMTPEMVADAAKAFRPKILYLY
                     HTSGTDTGRLVNLLRDAGGIEVRTKNP"
     gene            42016..42090
                     /locus_tag="PLW16_02285"
     tRNA            42016..42090
                     /locus_tag="PLW16_02285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42048..42050,aa:Gly,seq:ccc)
     gene            42219..42704
                     /gene="rimP"
                     /locus_tag="PLW16_02290"
     CDS             42219..42704
                     /gene="rimP"
                     /locus_tag="PLW16_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPB69766.1"
                     /translation="MAETSGSYRGEIASLADVLLRAEGMELVDVECLRMKSRWLVRIF
                     MDKPGGVTVDDCALISEQLGDVLDVRDLPPGAYTLEVSSPGLDRPLAKAEDFLRFRGS
                     RIDCRLKEKVAGSRHLRGILRDCLLGEGPGVLVIEVDGERRSISRDLILKARLIYEEE
                     P"
     gene            42768..44015
                     /gene="nusA"
                     /locus_tag="PLW16_02295"
     CDS             42768..44015
                     /gene="nusA"
                     /locus_tag="PLW16_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPB69767.1"
                     /translation="MFPELKRLIEQMGKDRGIDKQIIVEALEVAMETAARKKMGPRVE
                     IEAHYNDEAGEIEVFQFKTVMERVLDPEIQISIEEAREKWDEGAEPGDSLGMKIDAGT
                     FGRIAVQTAKQIIIQRVKDAERDNIYEEYKDRKGELVNGFVQRFEGGSIIVNLGRAEA
                     VVPVSEQIFREQYKRGERIRGYLCDVKKISKGPQVILSRTHPGFLKALFDVEVPEISE
                     GLIEIVNVSREPGKRAKISVRTKDKDIDPVGACVGMRGSRVQSVVQELRGEKIDIVPY
                     SDDPAKYVCSALSPAKVDKVFIDEDNRAMEVIVPDDQLSLAIGKNGHNVRLAVRLTGW
                     KIDVKSQSMATAQDEEGFKSLMKIPGVGEATAERLFKEGLKSVMAIAASDVETLSAIP
                     GVGEKSARQWIEAAGKILDQGVA"
     gene            44040..46727
                     /gene="infB"
                     /locus_tag="PLW16_02300"
     CDS             44040..46727
                     /gene="infB"
                     /locus_tag="PLW16_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPB69768.1"
                     /translation="MSKKRVHELAKELGIENKELIARLEKIGIAVKSHSSTLDEGDVE
                     RILKELKSSEPREVVEQRIKTGVIRRRSVRLPAEEAEPAPATEAPAEAEKSAAEALPK
                     EPVKPSTAPPAVPPAEAVQPKGAAPKRAPVSKAAPPAPPEPPTAGAEVRKVEAAAPAE
                     AAVAPEPTPKEKDMTPKPVPVARETETVSPTAPVPPAKAVPSPSARTAEKEVRKPAPS
                     RPDLMITRDAVPPRPEAPSARTEADKFKKRGKKPVEVLMEESAPAKKKVFAKKQFTDR
                     KARQIEVDTEERTPRWREEKRPAVVRMKKTEITVPKASKRRIKVGDAISVGDLAKKIG
                     VKASEVINKLMGLGMMATINQSIDFEAASLIATEFGYQVEPMGLEFDETLLKAETPAE
                     NLRPRAPVVTIMGHVDHGKTSLLDAIRETNIIGGEAGGITQAIGAYHVHIKNRDIVFL
                     DTPGHEAFTAMRARGAQVTDIVVLVVAADDGVMDQTVEAINHSRDAGVPIVVAINKID
                     KPGADSGRIKQALTEYNLVPEEWGGETIFSEVSAKQKIGIEELLELILLQADILELKA
                     DPDRPARGVIIEAKLDRGRGPVATVLIQEGTLREGDAFVSRTEYGRVRAMIDDKGKRI
                     REAGPATPVEVIGFSRVPQASSEFIGVEDEKKARSIGEYWIRKERERELSATAKITLE
                     QLYAKIKEGVKELNVILKADVQGSVEALVDALGKLGAADIKIKIIHSSTGAITETDVM
                     LASASNAIIIGFNVRPDARVAEVAETEGVEIKLYDIIYNVIADMKAAMEGLLEPEYRE
                     VSQGRAEVRDIFKVPKIGTIAGCYVTDGKLARSSGVKLVREGVVVYDGRIASLRRFKD
                     DVKEVATGFECGIGIENYNDIRIGDVIESYVQEEIERKL"
     gene            46777..47115
                     /locus_tag="PLW16_02305"
     CDS             46777..47115
                     /locus_tag="PLW16_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPB69769.1"
                     /translation="MVIGVGLIDILIPGSRSLKEKRGVLRRILKRTQNEFNISIAEVG
                     QQDLWQAARIGFCVVGNDRAYINGKMDHVFRFIDDLGMAERVRTKMEILSFADIGDDP
                     RLEMEKYGTI"
     gene            47102..47485
                     /gene="rbfA"
                     /locus_tag="PLW16_02310"
     CDS             47102..47485
                     /gene="rbfA"
                     /locus_tag="PLW16_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPB69770.1"
                     /translation="MAQYKRAERVADLIRAELADILRKRIRDPRIGFLTLSGVTLSDD
                     LRVAKVYFVEMGQDQCRPEIRAGLDRAVGFIRRELGKRIELRYVPELIFLYDPSFAYG
                     SRIERLIQEIHHQEEPEEPDAPEDH"
     gene            47466..48437
                     /locus_tag="PLW16_02315"
     CDS             47466..48437
                     /locus_tag="PLW16_02315"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPB69771.1"
                     /translation="MLQKIIDIIHRGRTFLITAHERLDGDALGSELALYAMLTDLRKE
                     AVVYNQDEIPGNYRFLPASETIVSALPPPETFDAAFILDCSELDRVGREAPRIAGIRQ
                     LVNIDHHVSNGGFCDTVLLDPKASSTGEILCRVMTAMNWEPTPSAATCLYTAILTDTG
                     GFHYGNTTPEALTAAAGLVVRGADPQRISENVYESNPLAKIRLLGAVLETLSLDGDDR
                     VGSLTVTREVLARTGAAPEHTDGLVDLPRTIRGVDVSVLFSEMEDGRIKVSLRSKGRV
                     NVERVARAFGGGGHVNAAACRVEGDVEAVKRNVIAAVVSSLGAEERR"
     gene            48434..49366
                     /gene="truB"
                     /locus_tag="PLW16_02320"
     CDS             48434..49366
                     /gene="truB"
                     /locus_tag="PLW16_02320"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPB69772.1"
                     /translation="MNGVIVIDKPAGKTSFDVVDEVRRTLGVRKAGHTGTLDPLATGV
                     LPVCLNEATKLVPFLMGGWKVYRATLLLGVRTDTLDREGQVERTCVPSATPEEIAGAL
                     EKCVGRRLQQAPRYSALKFKGKALHRWARQGVAIDPPSRIVEINELTIESIEGPYATF
                     TVRCSKGTYIRSLCAEVGESLGCGACLYALRRLKTGPFDETLAIRLEDEAGAAGPERL
                     RRGLIPLVDALPELTEIGVDASLAKRIRAGHQPKRGDWGEEDIPFLAPEDMIKFTLNR
                     RALVAIGKVVPPPQASGEASGDAPFTKILRVFNP"
     gene            49480..49746
                     /gene="rpsO"
                     /locus_tag="PLW16_02325"
     CDS             49480..49746
                     /gene="rpsO"
                     /locus_tag="PLW16_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB69773.1"
                     /translation="MLDAEKRKGIIGKYQIHENDTGSPEVQVALLTARIEYLTEHFKA
                     HKKDHHSRRGLLKLVGQRRRLLDYMKKKDVERYRSIIQQLGIRK"
     gene            49835..51970
                     /locus_tag="PLW16_02330"
     CDS             49835..51970
                     /locus_tag="PLW16_02330"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPB69774.1"
                     /translation="MSKDFRCDFAGREICLKTDYVAAQADGAVFAQYGDTVVLVTAVS
                     LKTAREGLDFLPLTVDYQEKTFAAGKIPGGFFKREGKLNEREILLSRIIDRSIRPLIQ
                     KGYFFETQIVATVLSVDDENDSDVVAMLGASAALTLSNIPFQGPIAGVRVGRLNGAFI
                     CNPSHAQQAESDLNLFLVGRKVLPGTEGRPYDVNLVMLEGEAREVDEDTLVDAINFGL
                     ESLRPVIDLQEQIRQEIGRPKRVFVPPAIDPDLAARVEAAARPGFTDGYATAGKLDRY
                     AKLDEIRAAVVKEFTAEAPDLKSQVLTILEHLERKILRGMILHEKKRSDGRSATEIRP
                     IRCDVGILPRAHGSGLFTRGETQALAALTLGTSSDEQRLDFIGGEEMRSFILHYNFPP
                     YSVAEAKSIRGPGRREIGHGNLARRALLPVLPAPEVFPYTIRIVSEILASNGSSSMAT
                     VCAGVLSLMDGGVPVKDVVAGIAMGLLKEGDEVVILSDILGDEDHAGDMDFKVCGTEK
                     GVTAMQMDIKIDGLTGDILRGALRQARDGRCFIIDRIRETMAAPRDDISVHAPRITTV
                     KVKPEKVRDVIGSGGKNIRQIISETGVTIDVEDDGTVTIASSDADAAARAIAMVKWLT
                     EEAEIGKIYTGTVKKVVDFGAFVEILPGTEGLVHISHLAKERVNKVTDIVHEGDEVMV
                     KVLEIDKQGKIRLSRKEALDAHGPENRSR"
     gene            51945..53198
                     /locus_tag="PLW16_02335"
     CDS             51945..53198
                     /locus_tag="PLW16_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB69775.1"
                     /translation="MVRKTVLDNGLTVVTEEIDTVRSISIGVWVRCGSRYESAADNGM
                     AHFIEHMLFKGTERRSAFDIAAAIDSVGGVMNAFTGKELTSLYIKVPDYHLPLAMDLL
                     ADILRQSRFAAEEIEKEKAVVRQEIHMLEDSPDEYVHEIFEKAFWGGHALGRSILGTK
                     DRVAGFTRDGLNAFFDRMYRGRNMVLSAAGHLDHAVFLKQVETAFGALPEGRRQAFPD
                     APTTEARQVVQEKDLEQVHLVVGTVAPAARSPERYAGFLLNAVFGGSMSSRLFQEVRE
                     KRGLAYAIQSYLVPYQDTGILGVYAGADADRVEEVATLIQAEMSRLRRTPLTADELQA
                     AKELIKGNFLLSMESTDHRMTRLAKNELCLGRQTTAEDVLAAVDAVTCEDIAHLAGEL
                     FRSETLSAAVMGPVRPKQAFDFRMT"
     gene            53209..53679
                     /gene="dut"
                     /locus_tag="PLW16_02340"
     CDS             53209..53679
                     /gene="dut"
                     /locus_tag="PLW16_02340"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPB69776.1"
                     /translation="MNVDALGEEDIEIPVQRLAGTEDLPLPRYMTERAAGMDVHAAVV
                     EDTVVPPGGRRLIPTGLKVAVPPGHEAEIRPRSGLAVRHGVTVLNSPGTIDADYRGEI
                     GILLINHGTEPFVVKRGERIAQMVVRRVGRVQWREASALEETGRGEGGFGHTRR"
     gene            53706..54896
                     /locus_tag="PLW16_02345"
     CDS             53706..54896
                     /locus_tag="PLW16_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPB69777.1"
                     /translation="MKKFKVQKSFQEINERIRRGKAVVVTAEEMIDIVERHGAVDAAR
                     RIDVVTTGTFSPMCSSGAFLNFGHTSPKIRASRIWLNDVPAYGGLAAVDCYLGATEMT
                     EADPLNSVYPGEFNYGGGHVIGDLVAGNLIRLRAEGYGTDCYPARRFEKTITIHDLRD
                     ATLFNPRNAYQNYSCAVNMSDKTIYTYMGMLRPRMGNATYATSGQLSPLFNDPYYQTI
                     GIGTRIFLGGAQGFVAWPGTQHNPRAPRGKNGVPREGAGTIATIGNLKEMAPEWLSGA
                     SMLGYGASLYVGIGIPIPILNEGLARFTAVKDEDLYAQVYDYGMDYPKGAAKSLGEVS
                     YRDLRGGEIVLNGKTIPTAPLSSYYKARQIAALLKEWIERGTFLLGEPQQLLPSIGEK
                     SDPS"
     gene            54993..55259
                     /locus_tag="PLW16_02350"
     CDS             54993..55259
                     /locus_tag="PLW16_02350"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPB69778.1"
                     /translation="MKVGRYLLVFVVFMALIISFGNRGLVDNYKMREKLQDLQRANQD
                     ISDANGELKRSIMLLRSDPATIERIARDELGMVKPGDFVYRFGK"
     gene            55416..56495
                     /gene="pilM"
                     /locus_tag="PLW16_02355"
     CDS             55416..56495
                     /gene="pilM"
                     /locus_tag="PLW16_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPB69779.1"
                     /translation="MVDIVGLFTGAKKLVGLDIGSSAVKLAEIVQTPKGFVLNNFHQT
                     PLPRGVITDGAVTEPDILTQHIKDLYKKAGCKRKAIVTSLSSHAAIVKKVNLAQMEEN
                     ELRELIHDEAGKYLPFDSMDDVLFDFQILGENEYNPNQMDVLIVAAKKDIVEGFTGAI
                     EKAGLPVVIMDVDSFALEHMYEQNYDFEETDVAALINIGASITNINVVLNGGSIFTRD
                     FSLGGNAVTEALQEKLGVTFEEAEKIKIEGVRGGEMEEREFNDSLLQLADPICSEIER
                     SLDYFRSTYGGGDIRKVLLCGGSANIPGIAQELSQRLSIETEVVDPFQKIAYNKKVID
                     PGQIESIGPVAAVGVGLALRRLGEK"
     gene            56492..57040
                     /locus_tag="PLW16_02360"
     CDS             56492..57040
                     /locus_tag="PLW16_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPB69780.1"
                     /translation="MIKINLLPYREKAAKAGMARQIILTAGFLIVLVLILAGTQIYLS
                     ANNARMEGEIKTAEEKLVTLNQKVGDVEKFKENKRVLERKLSVINGLEENRLAPVKML
                     AELSGLVPTRDVWLDKFTQRGRELQIEGVARDNTAVSRFMSSLERASFISAVELVSSK
                     EKEVSNVKLQQFTLTCQMKKGA"
     gene            57043..57771
                     /gene="pilO"
                     /locus_tag="PLW16_02365"
     CDS             57043..57771
                     /gene="pilO"
                     /locus_tag="PLW16_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPB69781.1"
                     /translation="MPITVDDIKKLPPKIQALILVGVVLLLGYFYYFFFLQATLEKRG
                     QLQTKLAELEQQVAEKERVAAQLTKYMREVETLEQQFKIALSKLPDKKEIPALLNSVA
                     LAGKGAGVRFLLFAPKPPARPVEKPGVKDNLKPSDKRAEEKGAAGSAGAPSAKAGGEA
                     FYDEIPVDVQIVGGYHNIALFFERVARLPRIVNIEDVVMGDAAAAADTKGRGRPLKTS
                     CIIKTYMFVERPDAAVKKTDQKAK"
     gene            57734..58330
                     /locus_tag="PLW16_02370"
     CDS             57734..58330
                     /locus_tag="PLW16_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HPB69782.1"
                     /translation="MLQSKRRIKKPSNPVTAIGVGLICCGLWLGAGVAAAADPGPLRP
                     SGPVPALAAKVKTAKTPEQVKAEKTPQAQFRYSPTGKADPFRPFLERDEIARKKAIKS
                     KPKPIPLSPLQRVGIEKFRLVGIAGGAARRVAVVEDAAGKFYTVKPGTIIGQNGGRVA
                     EIQEDRVIVEQTVSDQKKRTKVNRVVIQLRKEDYEGRL"
     gene            58327..60048
                     /locus_tag="PLW16_02375"
     CDS             58327..60048
                     /locus_tag="PLW16_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HPB69783.1"
                     /translation="MKRKWPWLWTSLLAASLILAGAVPAALAAAETSVGYVEEITAGP
                     LPGKERVTLVLSRMTAPTLEAVPGGGVLIKVPDMFIPEGLRSPLGEGALSNIRRIVPE
                     QRTVEGRQWAYVRVDLKQPVRYSVRQEGPSLIVDFNVAGLNEPPAATERTAAAAEEAR
                     PAAAPTAAAQEPLPGGDEKRYSGKRISLDFQDANIRSVFRLLSEVSGMSLVSGDDVKG
                     NVTVHMNNVPWDQALDVILDIKGLTKKQMGDVISIITLAKAKKDEADRQSKEEARIKA
                     EALEKEREQKLKQVKGKLRQISIEAKIVEAEEGFGRDLGIQWGVGKKSSFGSHYDYGV
                     VAQAANSAMTGDVASGGNVKGLAAGRALTAAGTALTAAGLAVNFPVAAAAPALGFVLE
                     GAHWILDAELLAQELTDRVKVISSPKVLTMEGVEARIKQGEQIPKVTPATAENPPTVD
                     YKDAFLELAVTPKITDDGRVSMKIRASNNSANYAKAIMQNPPINTSEVESNVVINDGD
                     TVVIGGVLKTTESKGGEGVPWISKIPLLGWLFKYESVQTGKRELLIFVTPKIVKDDAV
                     VAAASPA"
     gene            60302..61009
                     /locus_tag="PLW16_02380"
     CDS             60302..61009
                     /locus_tag="PLW16_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB69784.1"
                     /translation="MDDADQLQAGEPERNKRDLFLDQARVRLEQNQPRLALEMAEARL
                     RRLPDDIDARTILCHALIRMGRPDEAREMLRRVEGTLLDFASLYAVLGDCRLKEGFPE
                     EAAAYYRKFLAFNPPDAAAVREVSGKLDALTGDGDAGRPEDERPLRVGDIAPDFYTVT
                     LADLYLRQGHPEMAVDVLTRILEKDPEDVKARSRLREIQAVSEPRPVRRPGGHARTIG
                     TLSRWLVKLEASRPHAS"
     gene            60999..62093
                     /locus_tag="PLW16_02385"
     CDS             60999..62093
                     /locus_tag="PLW16_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB69785.1"
                     /translation="MPPEATPYARRRSRLSDRLPDWGLDALLLTEEANLRYLTGFAGT
                     DAALLMTESRASLLVDGRFLTEARETVPEVEIVEIRDLIEGIATALADAAPSRVGFET
                     AVLTVHVHQRLLAALGAGVSLRPLLDELSTLRAVKDEEEVRCMRKAAAIASSALQAIL
                     PDLRPGVAERDIALELDFRMRRQGAEGSAFPTIVASGPRSALPHATPGARKIRAGEGV
                     VIDYGAVFQGYRSDETCTVFVGGGPDREALAAYAQILEAQTLAIAGLRAGVTCRDVDR
                     IARDALRRGGWDRFFSHGTGHGVGLDVHEAPRLNARSESTLEAGMVVTVEPGVYLPGR
                     WGIRIEDMVLIRADGFEVLSRVPKTLTLLK"
     gene            62206..62601
                     /gene="gcvH"
                     /locus_tag="PLW16_02390"
     CDS             62206..62601
                     /gene="gcvH"
                     /locus_tag="PLW16_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPB69786.1"
                     /translation="MKVFPEDYMYSREHLWVRVDGDLATIGITDYAQEKLGEVLSIEL
                     PEIDTEVERDESFGSLESAKAVIELIAPVGGQVVNVNDDLLDDVGIINSDPHDTGWLI
                     VIEMSDLDEIDDLLDTRGYNDFILESEED"
     gene            62608..63453
                     /locus_tag="PLW16_02395"
     CDS             62608..63453
                     /locus_tag="PLW16_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HPB69787.1"
                     /translation="MSNWRLIPFHVSDAFENMAADEAIFRLMPLGHTPPTLRFYGWRP
                     AAVSLGYFQNTAQACNLAVCRREGIDVVRRPTGGRAVLHDGDLTYALCAGVDEPPFPP
                     GIAGAYRVIGRCLQEGLHRLGIDATPAPGNPAPPGVRDGSVACFARTARDELLVGGRK
                     ICGSAQARSRGAFLQHGSLLVRFDAARASRCLVAEAEERERTRRMLTRAVTAVDTCLA
                     PPPAMDAIVRVLAEGFAAVLHADLRPGALTDAEEALKARLKREKYRPEALNGEGEPDE
                     TAAPH"
     gene            63431..64507
                     /gene="hisC"
                     /locus_tag="PLW16_02400"
     CDS             63431..64507
                     /gene="hisC"
                     /locus_tag="PLW16_02400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPB69788.1"
                     /translation="MKRLHLIREEIRRQRAYPADATPCQIKLDANENPFPLPEDLTGL
                     FFSAVTREVALNRYPEAGSPALRRRFAARFGVAPNQVLLGNGSDELILALLIACGGPG
                     KSVLIPTPTFAMYRICALNLGCRVREAPLAAGGDLDLPAMLAALERERPVLTFLSYPN
                     NPTGGCFDDEKIAAVIERASGLVVVDEAYGPFSGRSFLPRLEAYDNLLILRTLSKVGL
                     AAMRIGFLVGPPEIVAEIDKVRLPYNLNSVSQMAAGFYLDHEARFMAQTEEIVRERVG
                     LWDALRQTAGMHPWPTDANFIFFTCDRDINQVYAALVKRGVLVKKFDASGSAPPAIRV
                     TVGRREENEAFLKALRAGVTTLGA"
     gene            64621..65727
                     /locus_tag="PLW16_02405"
     CDS             64621..65727
                     /locus_tag="PLW16_02405"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPB69789.1"
                     /translation="MKMILLLEDGTSFPGESFGAVGEATGWVHDERGVIGYQECLTDP
                     DNRGCIVNMTYPLIGNYGVNDEDGESDRAQAAALLVKEKSRVVSNWRAGSTLEAFMDR
                     WGVVGLEGVDTRALALHLREHGEMRGIVAPAASPTAALLAKVRADSGPLPADRPRGSR
                     SHAVSARPFETDGGPRIAMLDFGCRRSYLRQLEALSCQVRSFSPTAAWEEIRRSGPDG
                     LFLSNGPGAPEAFEPARRMLAAALGELPAFGIALGCQMLAIAAGGKATRMGQGHHGAN
                     YPVRDLRTGRTAMTLQNHGWVIDETSLKGGICTVSHRNLNDGTVEGVSSERGRFLGVQ
                     FSPVPDDDGRPSALFREFVERTTRTGGKRQKAKG"
     gene            65758..69027
                     /gene="carB"
                     /locus_tag="PLW16_02410"
     CDS             65758..69027
                     /gene="carB"
                     /locus_tag="PLW16_02410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPB69790.1"
                     /translation="MPKRTDLKKILIIGSGPIIIGQACEFDYSGSQACKALKEEGYSV
                     ILVNSNPATIMTDPEMADRTYIEPITPEIVEKIIARERPDAILPTLGGQTGLNTAVFL
                     ARAGVFEKYGVEVLGASAEAIARAEDRDLFKQAMLEIGVGVPDSGIATTVEEGMAIGL
                     GIGFPLILRPAFTLGGTGGSIAYNKEELEEFLAKAIEYSPVGQVLVERSVLGWKEIEF
                     EVMRDAADNVIIVTSMENVDAMGVHTGDSAVVAPAQTLTAPEYTHLTALCRKIIRKID
                     VTGGVNIQFAVSPDNGEVVIIEVNPRLSRSSALASKATGFPIARVATKLAVGFTLDEV
                     KNDVTGRTSALHEPTIDYCVFKISRFTFEKFPLADPTLNTSMKAVGEAMSIGRTFKEA
                     FQKGLRSLETGRFGFGADGKDVPFEGDGTVEKIREKLRIPNAERFFYIRYAFQAGLTI
                     DEIYDLTRIDRWFLFNMKEIFDIEEELVRYRFHGVKRDLPVWKKDFPAALLRRAKECG
                     FSDVQLAHILGVEEETVRRMRKKLGLNPVYKLVDTCAGEFKADRPYYYSSFETLEESH
                     PSDREKVIILGGGPNRIGQGIEFDYCCVHASLALRELGYESIMVNSNPETVSTDYDTS
                     DKLYFEPLTREDVLNIIEREKPVGVIVQFGGQTPLNLAVPLEKAGVPIWGTTPDSIDR
                     AEDRKRFQMLLKKLNLIQPDNGTARSYEEAQRVANKIGYPVIVRPSYVLGGRAMEIVY
                     DDEALGDFVKKAIRVSPEHPILIDKFLEDAIEIDVDALSDGETTIIGGIMEHIEEAGV
                     HSGDSASVIPPHTLDQRLVQVVKDNTYALARELHVSGLMNIQYAIRNDVVYVLEVNPR
                     ASRTVPFVSKATGVPLAKLATKVMAGRTLAEMGLTKEVEIRHVAVKESVFPFARFPGV
                     DAILGPEMRSTGEVMGIDVSFGKAFGKSQIAAGTNLPQTGKVFISVMNKDKRQIVFLA
                     KQMEHMGFSILATAGTGGVLKNNDIAVQILPKLSEGRPNVIDLMKNREVALIINTSGG
                     KKPRADTVFIRSTAVLHNVPLITTMSGAQAAVNAIEAMRKEAVKVRTIQEYHQEIGIG
                     R"
     gene            69221..69412
                     /gene="rpsU"
                     /locus_tag="PLW16_02415"
     CDS             69221..69412
                     /gene="rpsU"
                     /locus_tag="PLW16_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPB69791.1"
                     /translation="MEVKVFDNDVDKALKILKNKLSKSGLFKELKLRRAYEKPSVKKK
                     RKAIEARRRLAKVQRRRSS"
     gene            69587..70240
                     /locus_tag="PLW16_02420"
     CDS             69587..70240
                     /locus_tag="PLW16_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPB69792.1"
                     /translation="MEENQYLVDALSIEESWRIFRIMAEFVEAIDALSKVQRAVSIFG
                     SARTSPEDPFYQKAEYLARRLVEKGFGVITGGGPGIMEAANKGATEAGGKSVGMNIRL
                     PFEQKPNPYANISIDYRYFFIRKVMFVKYAVAYIILPGGFGTLDELFEALTLIQTRRI
                     KSFPVILMGTAYWQGLLDWLKGSLLANGMILPEDLDLIHVIDDPDEAVRHILKYVIV"
     gene            70279..71655
                     /gene="holA"
                     /locus_tag="PLW16_02425"
     CDS             70279..71655
                     /gene="holA"
                     /locus_tag="PLW16_02425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPB69793.1"
                     /translation="MVDQEGGSAPNLSRVLSDIASGCPVPCYLLFGEEEFLVKTAREK
                     LLNALLPPGERDLNLFSLDGETAEAGQIAATLMTPPLLPGRKVVLVEGTPLLQSKKTL
                     PDLEKSVREHLDRNPGRAIRDFLLLLHLAGWHLEDLAADGWKKISAEEWNRTIGGEGA
                     AEREAWLPRILDACLARGRDAVQGAEATEPLEAVLREGLPEGNHLILTAEAVDKRKRL
                     FQAIAKFGVALEFSPVKGEAKQKQRLQDVVREKIAQSGKKLTPKAWFALGEKTGFQLR
                     ATTSALDTLLTYVGDKVTVDEADVEAAVGKTREGTVFALTAALDRKDGREALFVLKDL
                     FDQGVNPLVILAVTAREIRNLIHARLLIDSGRFAEIEREPDYGRFQKQIYPEIKSVLG
                     VAGKKEGGLAGQHPYVVYQALKNARRFTAETLVDALEALAEMDLAFKSTPRDPALALE
                     RFVLRFCA"
     gene            complement(71667..72185)
                     /locus_tag="PLW16_02430"
     CDS             complement(71667..72185)
                     /locus_tag="PLW16_02430"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPB69794.1"
                     /translation="MHILIDGYNLIRQSPELLRHERTSLEAGRLALIQRLVTYQRQRG
                     HRITVVFDGWQGGSPFEERDRSGGVVIRYSRRGETADAVIRRLAREGREEVLVVTSDR
                     GIAEHLGRRGAVAVSSLEFADRLEKALLKDAAGPPDDPDAEDAPRGGKRKGMARRPSR
                     REKAVQARLRKI"
     gene            72526..73380
                     /locus_tag="PLW16_02435"
     CDS             72526..73380
                     /locus_tag="PLW16_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB69795.1"
                     /translation="MEIKNVCVLGAGLMGNGIAQVCAQAGLNVTMRDIEQRFIDGGMN
                     TIRKNLSRDVEKEKKTQADMDAILGRIKPTLDMKEAAGQADVVVEVVIEVMNVKKQVY
                     QELEQIVPAHCLFFTNTSGLSITEMAAITKRPDRFIGTHFFNPVPVMKLLEIIRGYET
                     SDETLAVASEWGKKIGKDVIVVKEAPAFVVNRILCSMINEAFFVLGEGLASAEDIDKG
                     MVLGCNHPIGPLALSDLVGNETLLRVVEGLHRELGDKYRPAPLLRQLVRAGRFGRKTG
                     KGVYEYKK"
     gene            73450..74589
                     /locus_tag="PLW16_02440"
     CDS             73450..74589
                     /locus_tag="PLW16_02440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69796.1"
                     /translation="MDFELTEELQMLKDMAYKFAKAEFPNMSHECDEGEKYTPEIRKK
                     AAENGLVGAWIPEAYGGAGAGMLGNTLITEELSKVDMGIGLNIVAATFGSESIFQYGS
                     EEQKQLYLPKLCNGEWVSAGAFTEPNAGTDVAGYKTRAVKDGSDYVVNGNKMFITNGT
                     VCDYMVTQCITNPDQKKHNSFSLIIIPADAKGVTRNKIHGKLGIRSSDTAEIALEDVR
                     VPQANLVGKEGKGFYQLMHFFDTTRVMVSGQALGISEACLEASVKYVKERTCFGAPLG
                     AYQMTQMKLTEMAIRIEALRGLVYKAAWLIDAGKPDYTLAAMAKFFGGQTAVFCANYA
                     VELHGGYGYIDEYDVQKWYRDAKILELYEGTKEAEIMTIGRVLQA"
     gene            74688..>75003
                     /locus_tag="PLW16_02445"
     CDS             74688..>75003
                     /locus_tag="PLW16_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB69797.1"
                     /translation="MKTRITELFGIKYPIVLSGMSWISVPSMVAAVSNAGGLGILATG
                     PMDPEQTRQAIREIRKKTDKPFGANATLLMPGAAANAKVLLEEQVPVINFALGKGDWL
                     VRE"
BASE COUNT        13806 a        22938 c        24958 g        13301 t
ORIGIN      
        1 accccgatga cgacgtgttc gacgtcatcg agatgatcct ggcggaaggc aagacatcgc
       61 gcttgcaccg ccggctcgtg caggagctcg aactggccac ggaggtgtcc gccttctcag
      121 ctcccggtca ccgctatccc aacctgttca tcgtggccgc gacgccgcgc agcccgcatt
      181 cgccgggcga ggtcgaacag gccgtcgaac gggaactcga acacctcatg accgagccgg
      241 tcacggagcg ggaacttcag cagatcctgc accggctgga atacgaggag gtcgtccgga
      301 tgtcctccaa caatggtctg gccagcgtcc tgaccgagta cgaggcggtg acggggaact
      361 ggcgctgcct gatcgagcat cgccgccggg tcgcagcggt cacgcccgcg gacgtgatgc
      421 gtgtggcgaa aaccttcttt acgaggggaa acaggacggt gggaacgatc gtgagaaagg
      481 aggcggcgcc gtgaaggcag ctctcagacg gggtctggcg ctggtcgtcc tcggagtcct
      541 gttcgccgct acgccgctcg gggccgcggc gcccgtcgat ccccggaccc tgaccttccc
      601 gcccctgacg tacgacattc cgtcgccgga gcggtttgtt ctggacaacg ggatgacggt
      661 cctcctcatg acggaccgcg cgttgccgct gatccagatc accgcctacg tcggcgtcgg
      721 cgccgtctat gagccggccg acaagtgcgg tctggcggag ctcaccggcg cgatgatccg
      781 gcgcggcggt ttcgcgggga aaacgcccga ggcgctcgac gccgagctgg agttcatggc
      841 ctccgaggtg gaatccgcca tcggcgacga cctgggaacc gtttccctga gaacgctccg
      901 caagaacttc gaggccacct tggggctctt cgccggggtg atgagaagcc ccgccttcga
      961 cgccctccgg ttcgaccaga ccgtcaagga ggccctggag acgctccgcc ggcagaacga
     1021 cgatccccag gagctggcgg accgggaact cctccgggcc atctatcgcg gccatcccct
     1081 cggccgcacc ccgacccccg ccaccctccg ggcgatcggg cgggaggatc tcgccgcgtt
     1141 ccaccggcgg tactaccggc cgaacaacgt cctgctcgcg gtgtccggcg acatcgaccg
     1201 cgcggaactg gaagaccggc tgaggtccgc cttcgacggc tgggagcccg cgccggtcga
     1261 ctttccgacc gttccctggc cggacatgac cctgcggccg gccatgctct acgtgaagaa
     1321 ggagcttgcc cagtcggtga tccgcatggg gcatctcggc atcacgaagg acaacccgga
     1381 cctctacgcc ctccgcgtca tggacttcat cctgggcggg ggcggactca actcccggct
     1441 catgggagaa atccgcgcca atcaggggct ggcctacaac gtttccggct atttcgaaat
     1501 cggccggaga tttcccgggc cgttcctcgc cgagaccgag accaaggcgg cgtccaccgg
     1561 gcgggccatc gccctgatgc tcgacatcat ggacggcatc acgaaggtgc cggtcacgga
     1621 ggccgagctg acgctcgcca aggagtccat cgtcaacgcc ttcatcttcg ccttcgccaa
     1681 tgccgagcgc gtggtcaccc agcgggcgcg catcgtctat tacgggtatc ccgaacggta
     1741 cctcgaggac taccgcgcca acatcgcaaa ggtgacgcgg gcggacgtcc tgagggtggc
     1801 ccgccggtat cttcatccgg acaaactcat caaggtcgtg gtgggcgacg cggcccgatt
     1861 cgaccgtccc ctttcggcgt tcggggaggt tcgggagatc cggccggacg cgtggaactg
     1921 acgggtcctg acggacccca tgccgggaaa ggatggaaac agggtgacga acagatctct
     1981 tccggacgcc ttcttcaagc cgggtaccgt ggccgttgtc ggtgcgtcaa agaaggccat
     2041 gttcagcagc ggcattccgg acacgcttgt caaatacggc taccgggacc gcgtgtacct
     2101 ggtcaacccc cgggaaacgg aaatcaacgg gatgcccgtc tatgcccgct tggccgacat
     2161 ccccgctccg gtcgatctcg ccatcgtggt tgtgccggcg acccaagctc cgattgtcgt
     2221 cagggagtgc gtcaagctcg gcattccagc cgttgttctc gaatcggcgg gattcggcga
     2281 gacgggccct gaaggggctc gtctggaaga ggaactgcgt cggcttctga ggggcgcgcg
     2341 gacgcgagtc atcggtccga attgcctggg ggtgatcaat ccgcatgaat cttttgcaac
     2401 gaccccggtt gatctgaagg cgttacggcc ggggaacatc ggtgtcatcg cccagagcgg
     2461 gaccttcggc aacattctcg cggactgggc gccgacgcag gatctctact tcagcaagct
     2521 tgtgaccatc gggaaccggc tggacgtgga cgaaaccgat tgcctcctct atttcgcaga
     2581 ggatgcccag acggacgtca tcgtcctgta cctggagggc gtgaaagacg gtcgccggtt
     2641 ttacgaggcg acccgagagg tgtcccgccg caagccggtc ttggtctata aggggggacg
     2701 atcgccggcg ggtcgcatgg ctgcggcttc acacacggga agtctgacgg gtgaagatga
     2761 actctacgat gcgattttcc ggcaatcggg ggtaacacgg gccgcgtcgt ttcaggaact
     2821 ttttgagatg gcccgcgtgt tctcgcggga gccgctcctg accgggccgc gcatctgcgt
     2881 tgtgacggca tccggctcgc tcggggtgat gatggccgat gcctgcatga ccggggggct
     2941 ggagcttccg ggtctgtcgc cggagacggt ttccgttttg cgtgaacttg ccccggcctg
     3001 gatgaatgtg aagaatcccc tcgatgtcgg cccctcgggt attttcgcgg ccgccatgaa
     3061 agccgtcgct gccgatccac ggatagacgg gatcatcgcc gttcccgttt ttcctgagga
     3121 gatcctgcgc aggtttgcgg cgatgggcat cgatacccgg tccatgaacg gcgacccccg
     3181 ggaatggcga aaccttgcgc cggacaagcc gttcctcatg ttcacggttg gtggaccttc
     3241 ctggctcgaa tccattcgca agacttacgg accggccgtt tccctggtcg gttcccccga
     3301 aatcgccgcc cggtctttgg ctgccctgta ccgctacagc tcttttcggg cccggggata
     3361 aaacggacct ctcccttcaa gccccttccc ggcatcccgc ggccgtcagg gatccgtttc
     3421 aggcagaaat catccgatga tccgtacggg cctgcagggt cctgatcaaa gatgcgggga
     3481 caggcttaaa tcatggggcc atgaacatgc ccttgggcgc cagacaatcc agtggattct
     3541 tttcggggtg gtacatatcg ataaagaggg tgaattcgct tccatcggaa tcggtcagct
     3601 tgtattgatc ggtgatgtgg ccgtccggac ctgcgccgac attgccgacc cggtagtatt
     3661 tgaacggccg gcgatttttg tcacgcaact gtttgagata cacgtgggag taggccgccc
     3721 ccgaacctga gtctccgccc aacttgaccg ggttctcccg ggtatagccg taggtggggt
     3781 cgtcggaggt ctgggccggg ttgatatcat cgccgccata ccgccccgtg tcggtttgcc
     3841 gcgcggcacg gtcgggagcg acattcggac ggatgcgcct tctgtcgcgg tttgcctgat
     3901 ccaccgcccg ggtcgcttca tcgaccatgt gttgcgcggc atccccgcgc aagcgccggc
     3961 gctgttcggc cccgtcttgc gcgaaggacg gaaatggcgt aagttcgaac gccgtgatca
     4021 agagaaaaag cagggacagg cagacggctt ttgttttcac gggcaacctc ccttgaagga
     4081 taggtcccgg actgtagcac tttttaacgg gattgccaag gtcgtgtcaa aagcccggtt
     4141 gcgtccgggc atcggtatta gatgaccgtt gcgcccgggg tcgtctcatg gtgtggccat
     4201 gctgaaccgt cgtatgatcg gtctattcct caagacagcc gtttgcttgg cgatgctgct
     4261 gttgtcgatg cctggaaacg tgtgcggcag cgacgcggtt acggcggatc tgctgcaggc
     4321 ccgctatgat cggatcaaca ggctcctgaa agacgggcgc ctcgatgacg cactgcaaca
     4381 ggccttgagg accctcgaca tggccagggg cctctacggc caggagtcag ccggaacggc
     4441 gcccgccatt gaccgggtgg ccgctgttta ccgggcgatg ggaaggttcg cggacgcgga
     4501 aaccaacctg atcgcggcgc ttgatctggt caggagaggg ccggacctgc cccccgaata
     4561 tacggtttcg gcgctgaaca acctggccgg actgtacagg gagatggggc gctatggcga
     4621 cgcggaagcg ttgtatctgc gggccgtcga catgcaaaag tccctccggg atgaaagcgg
     4681 ggagggtgaa gcggccctgc tcagcaatct cgcggttttg tatatgaacc ttgcccggta
     4741 tgcggatgcg gagccgctgt tgaagcgtgt tctcgcgatg gccgaaaaga aggctgagcc
     4801 ggaccgtctc agccaaagga tgaagatttc catggacggc cgtcggctgg agtgggggcg
     4861 gcgcatgctg aacctggcga acctgtattt cgagatgaag cgccatgagg acgcggaatc
     4921 cctgacaatg cgggcactgg ggatctttca gccgattctg ggggaagaac acccggaggt
     4981 ggcgcgcgtc aaaggcgatc tcggcgtcct gtattcggca accggtcagt acgcccgggc
     5041 ggaaaaactg ctgaccgaag ccatcaatag cgccagcacg gcctacggcg acaagcccca
     5101 acccgtggtc gccgagtatt atgtcagtct ggcccagttg tatcaggaaa tgggcaatta
     5161 ccgggacgcg ctgaaggttg ccggacacgc cctccccatc ctgcaaaaca ccctgggcgg
     5221 gagccatccc aaaaccctgc gctgccttaa cactattgcg gtcattcata tttaccgggg
     5281 ggaatatgaa caggcggaat ccgtcctccg gcaggtcctg gagacggccc gggcatcgct
     5341 gggatcgcgg catgaaagcc tggccgtcta tctgtccaac ctggggaatg tgctcgcggc
     5401 ccggggccgg catcgcgagg ccaaccggat gtttgccagc gccatcggca tcaacgaaca
     5461 gaaacgggag gatgcctttc tgatcctttc cgagcagcag aagcttaatt atgtgtcctc
     5521 catgctgcac cagatcaaac tttatctgtc ccacctgttg cagcatggcg tatccgaccc
     5581 ggaagcgctc ggggaggcca tgaccgtctg gctgagatgg aaaggggccg tacaggaggc
     5641 ccaggggcga tacggggatg ccctgtaccg ctccgacgat cccgtcctgc ggcggaagtt
     5701 cgatgaactt gtccgggtca ggcgcgagct ggggggcatc cagctctttg gaggacaggc
     5761 cgcccgccag cggggggccg ccgaaacggc cagccggctg gaaaagcgaa aacaggccct
     5821 ggaggccgag ctgagcgggc tcagcaggga ttatgcgttt gaacgcagga tcggcaaggt
     5881 cgatatcgcc cggttgacgg aactcctgcc gcccgatgcc gtgtatatcg attttgccga
     5941 gatcgccatg acggatttca gccggcagat gcccgggagg atgcactacg ccgctttcgt
     6001 tgtggcttcc ggtggcaagg ttccggtcag gctcttggac attgcggatc gggatgcgct
     6061 cgatgccgtt atcggcagct atttgaaaga gatgaggaag aaggccgcgg gacggccgga
     6121 ccacgatccg acgcgtctgg agagggatgc cgtccaactg taccggatgc tgctcaatcc
     6181 gctgcttcct ggtctgaacg ggcggacccg gctatacgtc agtccggacg gcctcctgaa
     6241 cctgatcccc ctggaggttc tgaagagcga aagcggccgc tacgccgtgg aagactaccg
     6301 gatcaactat gtcgcctccg gccgtgatct tgcccggttc gacacggttg atcctccggt
     6361 cggacgggtg gtgatcctcg ccgacccgga ctttgatttg aagcgacccg gacagggtga
     6421 cacgcagaca cccgccgttg aagcggccga agatggccgg cgtccggctg tcggcatctt
     6481 cagcggggtt cactttgaca gattgcccga tacgaaacag gaggcggagg ccatcgggac
     6541 ccttctgaag gcctcgggtt accgggtcga tctctacgag ggccgcgaag ccgttgagga
     6601 gaatctgttc gccgtgagac atccccggat cctgcacatt gccacgcatg gatattttct
     6661 gaacgggaat gccgcggcct ccgcggcgac ccggggggtg aggattctgc tccaggagcc
     6721 tgtcctgaca aacttgaatg accccgttca gaaccccatg ctgcgttccg gtgttgcgct
     6781 ggccggcgcc aatgcctcca tccgggtggg gacggatcac ggattggtga gcgccgaacg
     6841 ggttctgggg ctgaacctgc gcgggaccga cctggtcacg ctttcggcgt gcgaaacggg
     6901 cacgggagac atccgggccg gagaaggggt cttcggcctg aaacgcgcct tcctgatgtc
     6961 gggcgcgaag actctcgtga tgagcctgtg gaacgtcccc agcgccgaaa cgacgaccct
     7021 gatgaccgaa ttctacacgc ggatgtcgcg gggggccttg aaggcgtccg cgttacgcga
     7081 agccaagttg aagatgatga aacaaaaccc gcacccgttt tattggggag cctttatcat
     7141 ggtcggcaag ccggaatgat ccgccggaaa agaaacgagc tgcctcccga acaggcaaaa
     7201 aaacggggga acccccttgc cgccgccggg atccgcaaca ccacgatccg ttggggcggc
     7261 aaatgcgttt ccgatggatg ttgacaaatt caggatgccg cgtcagaaga atttcgccgc
     7321 cagatcggtg gaggatttcc ggccgatgtc gtcgtagtgg ttcgcgagga attccccggc
     7381 cttttcccgt ccggtctcga acaggtattt cagaaattcc atgtccagat tcatcttgct
     7441 cgaggccgtc agcttggaca tctgttcctc ggcatcgatg gtgtggatga acgtgtgctt
     7501 gtatttggcc ggatccagtt cccccttttc gatcagggac gtcacgaaat ggatggccct
     7561 catctcgcgc atgaggctgg agttgaagct cagcgtgttg atccggtcga agatggcgtt
     7621 ggcgcttcgg ggcacctcgt gaatgttgat cggattgatc tggacgatca ggacgtcctg
     7681 gcagtccgtg ttgtagatga cggggaagat gggcggattg cccatataac cgccgtccca
     7741 gtagtactcc ccgtcgattt cgggcgcctg atacaggtag ggcaggcagg cggaggccag
     7801 gatcatcttc ggcgtgatct cctggttttc aaaaaccttc agccgcccgg tcaggacgtt
     7861 cgtggcgcag atgaagagcc ggacggtgtg cgccctctgc agcaccggaa agtcgatcag
     7921 ctcctcgagg atgtcccgaa agacgttcgt atcgttgggg ttccactggt agggggaaaa
     7981 catccgcgag aggacgtcga acgtctgcca ccagggggag aattccatcg accccttgct
     8041 gccgtagagc ttgtcgaaga aggtcggctg gaacggtgaa catcggccgt ttttggagtt
     8101 gaattcccag aaccggcgca gaagctcctg ggcctttgtg cgcccgcccg cgatcaggcc
     8161 gtaggcgaga atggaggcat tgacggcgcc ggcgctggtg ccgacgatgc cttcgatgtt
     8221 gatgcgctcg tcgtcgagca gacggtccag aacgccccag gtgaaggcgc cgtgggctcc
     8281 cccgccctgc agcgccaaat tgatcttttt cgcttcggct tgagcggtgg tccgtgcggg
     8341 cttcttcttc atggcattcg attccggggt tgtttttgtc gcccacagta agcgacggtc
     8401 gttgatctgt caaggataaa ggcaggataa cggctatcgg ccgatgcgcc gcccctacac
     8461 gtcgatctcc tcacgcgcct cttccggcgt ttcgtagata cggggcgaca tgcccctttc
     8521 gttgagcttg tcccccagct tcatgcgcag gaacgccccc gtggaatagc ggctgacctt
     8581 gtcataatac ttgccgacca cgtagctgac catttccgtg taatagtcga ccagttccgg
     8641 gcttagatcg aaattgtcgt agttcacgat ggtgcgaacc tttttcccca ggggttccat
     8701 gacggccgac accgccgcgc ggatctcgtc gatctgctcc cggctgcgga tcgccaggcc
     8761 ctcgaaattg acgaagaaga ggttgtcctc cggctgatag cgcaggcgat tcacgatcgg
     8821 gatgtccagg aggtccttct tgagctccat gggcacgggt ttgaacaggc ggtgatccat
     8881 caggcggggg gcctggcgga tgacgggctt gaagtccatc tgccccacga tgtcccgttc
     8941 cacatcgatg cccggcgcca cctcgatgag ctccaggcct tcgggcgtca gcctgaagac
     9001 gcagcgctcc gtgacgtaga gaacgtactt tcggtccgcc gcggccacgg cgccggaaaa
     9061 ggtgacctcc tccacccgct tgacgaattt gtgcaccttc ccctcctgga cgatcttcat
     9121 ctcgccgtcg cggaggtcga tcttcaggcc gccggcggtg aaggtcccca tgaagaccac
     9181 cttccgggcg ttctggctga tgtcgatgaa gcccccgcac ccggcgatcc tggggccgaa
     9241 acggctgacg ttcaggttgc ccgcctcgtc ggcctcggcc aagccgagga aggccacgtc
     9301 cagcccgccg ccgtcgtaga aatcgaactg gtggctcatg gcgatcaggg cgtccacgtt
     9361 gacggcggcg ccgaagtccc cgccgccggc gggcacgccc ccgatgatgc cgggctcgga
     9421 cgtcagggtc aggtattcct ggattccctc ttccgccgcc acgctggaga tggattccgg
     9481 cacgccgatg cccaggttga cgatgctgtt gggcttgagt tcgaacgcgg cccggcgggc
     9541 gatgatcttg cgctcgttca gggggatggg aggcaccagg gtcacgggga tccggtattg
     9601 atgggcgtaa gccggattgt agagggttgc cgccgtctgg cggtggcgct ccgcgttcgt
     9661 gacgacggcg acgcagtcca cgagggcgcc ggggatgatc acgctgcgca tcggcagggc
     9721 gccccgctcg gcgagtccct cgacctggac caggacgaat ccgccgcagt tcttcgccgc
     9781 cgtggcgagg gagagggcgt ccagatatag gggctccttc tcgaagccga tgttgccctc
     9841 gagatcggcg gtcgtcgcct tgatgatggc gatgttgagc cgggggggct tgtaggcgag
     9901 atattcctgg ccgtcgaagg cgatcacctc cacgaggtcc tccttcgtgt tcgcgttgag
     9961 tttccctcca ccgaagcggg ggtccacgaa ggttcccagg ccgaccttgg tgatcgtccg
    10021 cgggcgcccg ccggcgatgt cccggatcat gacggacagg acgccctgcg gcaggttgta
    10081 gccttcgatt ttcccgtcca gggccaactg ggtgagcttg ggggtcttgc cgaaatgtcc
    10141 gccgatgacc tttttgacca ggccctcgtg cgcgagatgc tgcaggccca tctcgttgcc
    10201 gtctcccagg gtcgaggcgt agaagaacgt gaggttccgg ggatggcccg tttcgagaaa
    10261 tttcttctca agggcgcaga tgatttcgtc gggacagccg atcccgatga tgccgttgaa
    10321 gccgatcaca tcgttgtcct ggatcaggtc ggcggcctcc tcgatgctga cgatcttgcc
    10381 tcgccgcatg cccgcggtga ggtcccggtg cagggggtgc tgaatatccg gcatggcctc
    10441 ctccttgagg gatttcctga agcgatttat tttcccggtc ggcgaaaaac tataagaagg
    10501 tcgacggggc ttgtcaatcg aatatcggcg cccgaccggg acggcctccg gccccggggc
    10561 ggcgggatcg tctccggggc gtttcgcccc gggaggggtt caggcctccc ctttgccgcc
    10621 gggcttgtcc gtgtcgtgaa agaggtcgaa aaaagactgg aaggccggga gcgacgcgat
    10681 cgacgtctgg gtcatcttgg agaagtccat cccgaggctg agccatttcc ggaactgttc
    10741 gtccgcggcc gccttgtagg agaggtagct cttcagcgtc tgctcgagat ggtggtcgaa
    10801 gaattcgccc aggacggttt caccgtactg aatgatcaga tgcaaaaggg gaaccggcag
    10861 caggctgttt tttttcctgg attcctccag gatgatctgg gtgagcgtga aggccgtgac
    10921 gtcttcgccg gtttttgcat cgacgacttc cacgcgacgc ccctgacgga tgacctgcag
    10981 gacgtcgtcc agggtcacgt aggcgctcct ctcggtgtcg tagaggcgtc tgttggtata
    11041 tttcttcaag tgcagttttt cggtcatcgt tcctttcctt tctccttgcc ttcatagcgg
    11101 agaatcggtc acaatgcaac aaaaaaatgt cgctggggaa gataaccctt tttgtattcg
    11161 gatcggtcgg ttcgtttaac caatcgatca tacgaggaat atctaatctc gctcctctcg
    11221 gtgtcgaagg ggcgaggggg ataatcatct tattgcaatg caacattttc cgcttgacaa
    11281 atcttttggg tgcgctatat taaggcccac gtcgggcagg aatgagaaaa cacaaaaagg
    11341 aggaaaaagg catggaacag gaaaaactga cgaagcagat gattgaattt cacaagacga
    11401 cgtttgacaa caccttcaat gccgtggaga ttctccagga gcagacggaa aagatggtcg
    11461 gcatgttctt ggaccaggcg ccgtggttcc cggcagaggg gaagcaggcg gtcaacgaat
    11521 ggctgaagac ctacaaggaa gggcgggaga cgttcaaggg cagcgtcaac gaaagctaca
    11581 agaaggtgga agaatatttg ggcgctctcg agaagaaggc ccccaggacg aaggcgggca
    11641 aataggatcg ctcaacgaac cccgtccggg atgcgacggc cggaggaggg ggcaaaccct
    11701 ttgaaaggag aatgggacat ggaaccgaaa cagatggcga agcagatgat ccagttcaac
    11761 aaggccgctt tcgacaacac cttcaacgcg atgacggtgc tgcaggatca ggcggagaag
    11821 atgatgaaca gcctgatcga tcagagcgcc tggatgcccg cggaaggcaa gaaggccctg
    11881 tctgactggg tcaaggccta caagcggggt cgcgaggact tcaaggccgc aatggacgac
    11941 aactacaaga aggtcgatgc gttctttgcc ggcctggaga agggcaagag cgcctaggga
    12001 cgccggtgta aatcccctcc cgacaggggt ctaaccgggg catgacgagg gtcatccccg
    12061 acatcgcgac gacgaccgcc gttgcaccgc cgcatgtccg ctctgccgga gaatggaaac
    12121 gtcatggacg caagattcta tgagttttgg ggccggttct tctcgtccct ggcgcaggga
    12181 cggaacccct gggaggattg gattcccggg cggaccccgg tggccggcgg cgccgacgcc
    12241 ttctcggaat tcttccgcgg gatggacgga ggggagcgcc ggccggaagg cgacctgccg
    12301 ggcggggagc tgtggcagga ggcggcggag gcctttcaga attcgcttcg ggacttcctc
    12361 gccctcttcg acgtggtttc ccgggaggat tatcgggccc tgcaggacga atgcgcggcc
    12421 ctgaaggaaa cggtcgcgag ccaggaaaag accatcgcgg gcctgaaacg gcggcgcgga
    12481 agaaaaacgg gcgcctcccc ggaggaaacg gtgaaacggc tccgggaggt catccgcagg
    12541 cagaacgagg aattcgagtc gctcatgaag gcggcggccg gtctctttga agggaaaccg
    12601 acatcctgaa cggcgaacga ccgggcgcct cgaccccccg cttcaggggc ctgcgggcgg
    12661 gcggtcttac ggtttcgcct tgggcgggct gaccagggcc tcgccgtcga gaacgacggt
    12721 gccgtcctga ttggtgcacg tggttttcag gcggacgcga ttcttctccg ccatcacttc
    12781 gacgacctcc gcccgggcgg tgacggtgtc gccgaagcgg accggcgcca gaaagctgag
    12841 cgactgcttg aggtagatcg tgccggggcc gggaagctgc atccccagca ggttcgaaat
    12901 gaaaccggcg gtgaacatgc cgtgggcgat ccgggtcttg aagaaggtgt ttttggcgta
    12961 ggcctcgttg atgtgggcgg gattcagatc ccccgtgatc ccggcgtaaa ggtagatgtc
    13021 cgtttccgtc acggtcttcg aaaattcggc ggcgtcgccc accttgatcc gatcgatcga
    13081 cttaccgttc atgttcccct gtcccccttt tccgatcgct cctaaaacca gcagcggtga
    13141 ctccagattt catccttgag ttccttcatg tcgcttttga gcatgaggta gaggccggcg
    13201 ttctggatgg ccagcccccc ctggtaggcc agggcggtga ccagcaggac ttcgtcctcc
    13261 gtgaactcgc gggcgacggc gctgtacaga cgcagaacgc cgatcacccg ctcctttgac
    13321 ttgatgggaa cgctcaggat ggtgacgatg ccttccgctt tcttctcctt cttgtactgg
    13381 acccccttgt ccgtcgaggc gttccttacg accaccggtt tccccttcag ggcttcggcg
    13441 atgctctttt ccgcggaaac cggccccttg ttgaggtacg tctcgctgag cccgcaactg
    13501 gcgacgagtt tcagggtatt gctctcctcg tccagcagcc ggatcgaggc cgccttgacc
    13561 ccgatggcct gggagacctc gcagctcagg gtctggagga tgtccttgat gtcgaggctt
    13621 gagttgatgc tggcggccag atcgaggaag atccgggtgt tctcccgcag tctttcgacc
    13681 agccgcgcgg tttcgaccgc caccccaccc tgttcggcga gaaccgtcag gaattcgatc
    13741 tccccggcgg agaaattcct caccttggcc gtatacagcg agagggtgcc gatgaaacgg
    13801 cccttgacct tgaccggcac caccagaatg gaggcgattc cttccgcccg tttcatctcc
    13861 cggttttcca ccacgggatc ggacgcggcg tcgcggaagt ggaggtatcc cttcttcagg
    13921 aggagcggga tttccttctt gacgtgcgcc aggcccgcat ggacataatt ttttgaaagc
    13981 cccgtctggg caacggggac gtaggttccc ttctcctcat ccatcaggaa aaggcaggcc
    14041 gccttgcccc ccatggcctc gaggacgccc tgaacgatca gttgcaggat cttgtcccgt
    14101 tccagggtcg ttcccagggc ccggccgatc ttgacgatgg tcctgaaata attgtcttgc
    14161 ttggtcatgg ggatctctcc tgtctcgttt ctgcaatccg ctgcggggat aaggctttcc
    14221 tccgaagacc gctttattta ttcgccgccc gctgtgccgg ccgggccctt cccctctccg
    14281 cttttttctt cttcgggggg cgcgccgggg ccggccggcc cttcccacgc gccgctttcc
    14341 cgggggtgaa cttgacgatc gccgcccctt tcgatgccgg tcgggcgtcc ggcccgtccc
    14401 tttccatcag ccagcgcgcg atcttgggtg caaattcccg ctgactcttg gagctgacat
    14461 agatgccgat atggccggta tcgagattga cgtcctccgt atcccggctg ccgacgtgcc
    14521 gggtgagctg ttcgcaggcc gccggcggga ccaggtgatc gtacatgccg tagaagttca
    14581 gaagcggcat cgtgattttc ttaagatcga cccgtttgcc gcccagtttc atcttgctct
    14641 ggatcaggag attcttcttg tagcagtcgt tgatgaactg ccggaaggtc tcgccgggga
    14701 cgtcggggct gtcgaagatc cacttctcca tgcggacgaa gttttccacg aaggacttgt
    14761 tgtccgcgtt ctccaggaaa cccacgtatt tgtcgatcat gaggcgggcc gggttgagca
    14821 gcagaaaccc caggttcatc agatccgccg ggatgttgcc gaaggtgtcg accatccggt
    14881 cggcgtcgag ggccttcatc cagatgtgga gcaggccggt gtcggtgtcg aagtgggtcg
    14941 gcgtcaccgt cgtgatcagg ttcttgacct tttccgggtg gagcgcggcg tacatgacac
    15001 agaacgaacc gcccatgcag attcccatga ggttgatccg gggcaggttg tgcttcctgc
    15061 ggatgaaatc catcgcatga tccagatagc cgttgacgtg atcgtcgatg gtaagaaacc
    15121 ggtccttccg ggagggatag ccccagtcga tcaggtagag atcgatgccg tcccggagga
    15181 aattcttgac cacgctccgg tccggctgga gatccagcat ggtttcgcgg ttgatcagtg
    15241 cgtaaacgac cagaaggggg gtcttgagcc tggcctcgcc ctcgaggcga taatgcttca
    15301 gcctgacgcg gtcttcctcg tagacgatgt catagggggt gggcgcgatc tcggtcgaca
    15361 gctcaccgag caggacctcc gataccttgg tgacccgcgc ctgtgctttt tccgtgtctt
    15421 cggccaggct ggccagaatg agatcgaggg gaatcttggg ctgatccata agcgtttctc
    15481 ctcaccgggc ggccgcgcat cctcaatccg ggttcaattg cctctccagc tcccgcaccc
    15541 tctttttcaa ctggtgaaga tcccggtaga gcgcgtcgac ttccttgtgg gtgggtacgg
    15601 gcaggagctg caggaggtcc tggacaacct cgtttttggc gatgatgaat tccgacagcc
    15661 ggttgaacag ctcgccgagc gcctggctgt actccggcga cttgaagagc gtcatgaaat
    15721 ggccttcgag gatcttgacc cacaggttgt agtactccct ggcctgcttc ggaactttcc
    15781 cctgctgcgc cagcttttcg atctcctgct gcatcacctt ggtggtctgc tcgaagggga
    15841 ggttgagcag ggaaaagaac tccgcgcccg tcgccttgag cagggcgagc ttgtcgagga
    15901 gcaggttcat ccgttcctga tagaaccggg tcagacccag ctgggggatg tagaagaact
    15961 gacggacctc tttttcgtag atgtctttca gtgtcttgaa gaactcctgg tccaggtttt
    16021 cgaagtggta ggcctcgacc ttccggccga tcttgccggc tttctcgatc tggccttttt
    16081 ggatctgaaa gcaggtgtcc caagaggctt cggcgatctt gagaaacagg tccgggaggg
    16141 cgtcaagact cttcgatgcg gcgccggcca cggccggatc gctcatcgcg gtggagagcg
    16201 actgccacat cctgagggcc gcttccatcg attcgaggga gcgcttgcgc ggtggtttct
    16261 gcgtggaagg ccccgccgcg gcctcggtcg ggggcggcac cattttcagg agcgaggtcc
    16321 aaagatccgc ccccgccttg atccagtcgg ggggaaaaag cccgtccggg gcttggccct
    16381 gctgagtctc gtccatcgca cacctcatga gccggcaacg ctgcccgggg gatagcgtct
    16441 ccccgtgcat tcatctgtct gcacgtgaaa aaaaccctac accgatccct ccaggcggaa
    16501 ggtcaggcgg caggattgag caggaacgac tccgaaacga cgattgatga atatatccaa
    16561 acgattcaac cgtcaaggga aaagatcccg gcatggtagg agctgcgcac gagcggcccc
    16621 gcctcgacgt gccggaagcc cagtcccagg gcgattttcc tgaattcggc gaattcggcg
    16681 ggggggtagt accgggcaac gggccagtga cggcgcgtcg gctgctggta ctggccgatc
    16741 gtgacgcgct ggcagcggac gacggccagg tcgtggagca gggcgacgat ggcgtcccgg
    16801 gtctcgccca ggccgatcat gaagccggac ttgctgacga tgtcccggtg cgggtgaagg
    16861 gcggccagca gcctcaggga gcgtttgtag tccccctggg gacggaggtt aggaaacagg
    16921 gatctggcga cctcgatgtt gtgattgatc acctccggtc cggcgtcgat gatccgctcc
    16981 agcgcctcgc ggcttccctg gagatccggg atcaggacct cgatgcggca gtccgggagg
    17041 cgcgtgcgca gcaggtggat gcaacgggcg aaatgttccg cgcccccgtc gggcaggtcg
    17101 tcccgggtga ccgaggtgat gacgacgtag cggagcccga gggcgcggac ggcctccacg
    17161 aggcgctccg gttcgtcggc gtccaccggc gtgggcgtcc cctggcggat gtggcagtag
    17221 cggcagcccc gcgtgcaggt gtcgcccagg atgaggaacg tggccgttcc cgcccggaag
    17281 cactcgcccc ggttggggca gagggcttcc tggcagacgc tgtggagacg gagttcggcg
    17341 agggtgcgcg cgatccggcg gtccgcctcg ccgcccggca ggcggacctt gagccacggg
    17401 ggtttccgga gagggtcaac ggctatttta ccattcatca tcaaaggcct tttcgcactc
    17461 aggatccgtg atcccgttag cgccggggag atttttcggg gacccccgcc tccaaagcgc
    17521 gtcgaggcga tgcgggggct aaaaggtgcg gcgcgcggag ggaccgcggg tctggaagcg
    17581 ctctcagaag tagtaccaga tcgacttgta gtcgctagtg ttttcgccgt ccgcggccag
    17641 ccgcttcagg tagtcgtaga tggagacgtc cgtgtagaga tagtcgaaat tcgtcgtgat
    17701 ccgcgcctcc caggtgtaga agcggtacac tcggcggccg tcgggaagga ccatgatgag
    17761 ctcgtggtcc tgccaggccc ggaggttcga tttccccagg cgggggacgt tgaagaccgg
    17821 ttcgtccgtc cggacgacgc cgggcaggag acgggcctcc tccttccgct cctgctcgat
    17881 gcgggccagc gggacccgga agtccacggt ctcctccttc cccatcgtgt tgaaggtgta
    17941 gtaggggtcg attccggaga gcttcagcat ccgccggagc aggcagagtt cgaagcggcg
    18001 gctgttgtag tacgtgaaga cgtgctgatt atagacgttg acgcccaggg ccttgatctt
    18061 ttttacggcc tcgagggcct cgggcgtcag ctccacggcg tgctcgaaat gggtagagac
    18121 gcagatctcc cggcggcccc attcgtggta tttcctcagg atctcgagga agccgtcatc
    18181 gatccggaag ggaagggtga ccggaacgcg ggtgctgatc cggatgcgct cgatgtggga
    18241 gatgcaggcc agttgtccga cgatccattc caggcggcgg ttgtccaggc acagggggtc
    18301 gccgccggtc accaggacct cgctgatgtg gtcgttgtcc cggatccacg cgacggcctc
    18361 gcggagcgcc tccgggggca gcatcgccgg ggcgtcgatc tgcttcacct cccagttgcg
    18421 ctggcaatag acgcagattt gcgggcaggc gttgaagggc ttcaggatca cgaccttcgg
    18481 gtaccggcgg gtgatcccgt cgacggggct cgtcgagcgc tcctgcatga agtccatgtc
    18541 gacgccctgc tcccggttgg ccgcgacgct ggcgcagtgc cggacggacg gcaggacctg
    18601 ggcgcggatc tggcggtcct cgtcggtgcg ccccgaggcg ttgaacaggg acaggtaata
    18661 gggggtgatc tccacgggga tgcccttttc cgccgccatc gcgagccccg ccgcctcgtc
    18721 gtcgtcgaga cggacgagcc gggagatcgt gtcgagatcc gtcaggatgt gcttgagatg
    18781 ccaccggggg tcccgccagt cggcctccgc ggcgccgaag tgcgcgagaa tcttcgcctt
    18841 gagcttccgc cgctgccgaa tcagggcggc gtcgaatccg gtccggtagc gacggcggta
    18901 gcgccgcatg atggccgagt aggcgtcgag ctgtcgcgaa cggaccgtcg cggcggaacg
    18961 atcctgggcg gccgaccggc cgtccgcggt cctcaggagt gccggaattt tcggctgctt
    19021 gccggccttg ccggtgatgc ccttgaagaa ggcgaggtac tcgcacagga aggccttgcc
    19081 gacccgctcc agcgcccccg ggcggttccg ggccaggtcg tacaggagcg tcagcggcga
    19141 ggcgccggtc aggtgttcgt tttccgtccg gatggtgttg gagaagacgc ggaggcactc
    19201 cttggcgttt ctcttttcga tggaattcat gccggcgtag ggggtgtccg gcttcatgtt
    19261 gaacatcgag cggttgagct ggttgaagtg gtcgaacagg atatggcgcg cctcctcgac
    19321 gtgcttggag ccggcgagga tcctccggat ctccgggtcg gcggcccaca gcttctcgac
    19381 gagttccttg ttgtccagtg agagaatgtt tttgaccatg agacgggccc ccgtggtttc
    19441 gatcgtcgag ggacattttg gccgaagccg ccccgaacgt caatcatttc ctgtttccta
    19501 agataaaggt cccttcctga ccggcgtgat ctcatccctc ccgcgctccg tccggaggcc
    19561 ggcttcaagt tttccgatgt ccccggcggt ttcccgggtc gtggatcagg cgggaccgga
    19621 gggtctcttc cagcagacgt ccggccggcg ggcggccagg acctcgtcga ggcgcttcac
    19681 gggggttgtg tgcggggccg atttcacgat ctccggttcg tccttcgctt cggtggcgat
    19741 ggcgatcatc gcgtcgcaga aggcgtcgag cgtttcgagg ctctcggtct cggtcggttc
    19801 gatcatgatg gcctccggga cgatcagggg gaagtagatc gtcggtgggt ggacgccgta
    19861 gtcgagcagg cgcttggcga tgtcggtcgt gtggacgccg ctttcccgga cctgccgccg
    19921 tccggaaaag acgcattcgt gcatgcaggg gcggttgtag gggaggtcgt agaaggtctt
    19981 cagacgcgcc tggatgtagt tggcggcgag gacggcgtcc tccgccgtct gccggaggcc
    20041 cgccgcgccg aggctgcgga ggtaggcgca ggccctgacc atcacggcga agttcccgta
    20101 aaaggcccgg acgcgcccga tcgtgtcggg atacgcctcc gaccaggcgt agcccgcgtc
    20161 cgtccggacg atccggggga cgggaaggaa gggttcaagg gcggccccga caccgacggg
    20221 gccgctcccc gggccgccgc agccgtgggg ggtggagaag gtcttgtgga ggttcaactg
    20281 gacgacgtcg aagccgatct cgcccggccg cacctttccc aagaaggcgt tggcgttggc
    20341 gccgtcgccg tagaggaggc cgccccggtc atggatgatc cgggccaccg ccgcgatgtt
    20401 ccgctcgaac aggcccaggg tattcgggtt cgtcagcatg aggccggcgg tgttttccgt
    20461 catggccgcg tccagggcgt cgagatccac gccgcccgcg tcgttggacg gcacctgcac
    20521 gacctcgtat ccgcataggg cgcccgaggc gggattcgtc ccgtgggccg tgtcggggat
    20581 caggatttgc gtgcgttttt cctcccgctt ctcgaaatat ttccggatca tcatgacgcc
    20641 cgtcagctcg ccgtgggcgc cggccgccgg ctgcagggtg aagtcggcca tgccgaagat
    20701 ttcgcacaga tcccggcgca ggccgtccat cagctgcagg ctgccctggg cgaaggccgc
    20761 cgggaccccg gggtggaggt cggcgaaacc gggaagtgcc gccgccatgt cgttcacctt
    20821 cgggttgtac ttcatcgtac aggagccgag cgggtagaag ccgaggtcga cgccgaagtt
    20881 gcggcgggac agggcggtat aatggcggac gagatcaatc tccgccactt cggggaggtc
    20941 gacgtcgtcc cggagcaggt gcgcctcgat gcggggttcg tccgccgtcc ccggcacgtc
    21001 gcacggcggg atcgcgccgg cccgccggcc cggccggctc ttttcgaaaa tcagctcaac
    21061 gttcggatgg gagggatgct tttccacggt ttcttactcc gctcgctcga tctaattcaa
    21121 cgatcccgaa acaatcacgg atcggccgtt tttccattcc cgcgcaggcg ggaatccggt
    21181 ccttcgctta cgtcatggat cccgactgcg cgaaaacgac cgctttgacg gggggcgctt
    21241 catctccacg atggcgggat cgcgtccgct tcaaccggat cccttttccg tcgctccggg
    21301 ttgtcagggg cggagcacgt cgaccgcccg gtccaggtcc gcccgcgtga gcagctccgt
    21361 ggcgcacagg agcagcgcac cgggcagctc gggataatcc cccgcgaggt tgaccccgcc
    21421 gctgatgccg gccgccgccc accgggcgtt cgcgccctcc gggtcgggac agcgcaggac
    21481 gaactcgttg tagaccggtc cggagaaagc gggcgcccag ccgggcaggt cgaggaggcg
    21541 ccggcgcaga tacccggcct tcgcgacgtt cagctcggcc aggggcttca ggttcttccc
    21601 caaacagacg aggtagacgg cagcggcaag ggcgcagagg gcctcgttgg agcagatgtt
    21661 cgaggtcgcc ttctcccgcc ggatgtgctg ctcccgggtc tggagcgtca ggacgaaggc
    21721 gcgccggccg tcccggtcca ccgtggcgcc cgccaggcgt cccgggatct tccgcagaaa
    21781 cgtatccgtg gcggcgaaga tcccgagata cggcccgccg taactgaccg gattcccgaa
    21841 ggcctggcct tcccccacgc agaggtcggc gcccagcccg ccggcgggct tgagaagacc
    21901 cagagaggtg gcttcggtga agcaatgaat gaggtacgcc ccggcggcat gaacgagagg
    21961 cgcgacggcg gcaaggtcct ccaccacgcc gaaataattg gggctctgca cgatgacggc
    22021 ggcggtcgat tcccggacgg cggccgcgag ggcctcgagg tccagccgcc cgtcgcccgt
    22081 gaacggcgcc tcctcgaccg acagtccgtt cgcccaggca tacgtccgga ggacctggcg
    22141 gtacgcgggg tggacggagc gggcgacgac gaccccggtc cggccgaggg ttctggcgga
    22201 cagcaccgcg gcctccgccg tcgccgtggc cccgtcgtac atcgaggcgt tggcaacctg
    22261 cattcccgtg aggcgggcga tcatcgtctg gtattcgtag acggcctgca ggatgccctg
    22321 gctgatctcc gcctggtacg gggtgtaggc cgtggaaaac tcggaccgtt gcgagaggtg
    22381 tccgacgacg gcggggatgt agtgccggta ggcgccggcg cccgtgaggg tgatgtcggg
    22441 gaggcggttg cgttcgctca gcgccttcag atgcgtgaag gtctcctgct ccgagagggc
    22501 gggcggcagg tccagcgggc ggctcagacg ccatcgctcg gggatgtctc cgaacagatc
    22561 ttcgattgag gcgatcccaa ccgcgtccat catggttttg cggtcttccg ccgtgtgcgg
    22621 gctgtaatcc atcggcatct tcctcccggg atcagtgcgc ctcgtcccgg atgaaggcgt
    22681 cgtaggcggc ggcgtccatc aggccgtcgt attcccgcgg atcggacagc tttagtttcg
    22741 cgatccaccc ttcgccgcag gcgtcctggt tgatgagctc cggccggtcc tccagggccc
    22801 ggtttacggc gacaacctcg ccgctcaggg gggcgaagac gtccgagacg gacttaaccg
    22861 actccacgac ggcgacggct tcgccctggc ggacctgccg gccgagcgcg gggagctcga
    22921 cgaagacgat gtcggtgagc agttcctggg cgtgatcggt gatgccgatc gccgccgtgc
    22981 cgtcgccgtg tggcagggcc cactcgtgct cctgggaata tttccggtcg gttggattgg
    23041 gtttggacat ggccttctcc tgatcaatag agggtttgtc ggtttgccgg gtttaccggg
    23101 gccgcctgta aaagggaagt ttgaccacca cggccgggac gaggtggtcg cggatttgaa
    23161 tgcagatctc cgtccccggg tcctttgccg tcgcggatac catcgccaga ccgatggccc
    23221 gggaaagcgt cggcgaaaac gttcccgaag taacggcgcc gatcgcctcg ccgttccgcc
    23281 agacggggtg tccctcgcgg gcgattcccc gcccgaccat ctcgaagccg acgagcttgc
    23341 gttccacgcc cgccgctttc tggcgccgga gggcgtcgct cccgatgaag tccttcgccg
    23401 gatcgacgag ccagccgtag gtcacctcat ggggcgatgt cgtctcgtcc atgtcatggc
    23461 cgtagaggag catgcccgcc tccaggcgga gcgtatcccg cgccccgagg cccacgggct
    23521 tcagattccg ggcggcaccg gattcgagca gcgcatccca gattgcgccg atctcgtcgc
    23581 tcgcggcgta gatctcgaag ccgtcctcgc cggtgtaacc gctgcgggag acgagggccg
    23641 gccggtcggc cacccgcgtg tgcgtgaagc ggtagtacgc gagcccgtcc agggccgccg
    23701 gcgtcagggg ctgcaggatc gcctgggcca cggggccttg gagatcgagt ttcccgattt
    23761 catccgtccg gtccacgatt tccaccttcc ggaacccctc cgccgccttg acggtcttga
    23821 gccaggccgc gtccttgagc cgcgttccgg cgttggtgac cagcccgtag cggtccgact
    23881 ccagccggta gacggtcagg tcgtcgagaa tgccgcccgt ttcgctcgtc aggacgccga
    23941 gcttgagccg gcccgtttcc aggtcgccca ggtcccggct catcacgcgc tgcagaaacg
    24001 ccagcgcggc ggcgccccgg acctcgatct cgcccatgtg acagatgtcg aagagcccgg
    24061 cgctcttccg cgtggcgcgg tgctcctcga tgacgccggc gtactgaacg ggcatggccc
    24121 agccgccgaa atcgatcatc cgggcgccgg catggacgtg gcggtcgtaa agcggcgtct
    24181 ggttcatgag tcactcccga gtcggccttg aggcctgcct tgcggacccg cccaggccgg
    24241 gggctgagca ggcgccgggg ttggggacag gaccgcccga ccggtcccgt cagggcccgc
    24301 gggcgatcga cgcgaaggaa ccccggatct aatagtaacg gtaaagcagg gacagcagga
    24361 tcaggatgac gaccaccccg aaaaagatgt ttgattttct cagggccttt ccgcccacca
    24421 cgaccccgtc gcaggactgg cagaccgtca ggtgcgaggc gatgggcgcc ccgcagttgg
    24481 gacagcgcag ctcatctttc ggcgaggacg tcttggcttc ggcgcttttt ttgatctgtt
    24541 cgccgacgtc gtaggtccag cggcagtcat cggtcgggca gcggccccgg atcggcatcc
    24601 cgtccgtcca catgaaactc ttcccgcact ggggacattg gatcttgacc atcgcttgtc
    24661 tctccggaaa cgtttctcgg aatcgctcgg cgccgctcta cgtactataa gggagggggg
    24721 atttcaaggc aaaaggtcct cccccattat tttattgatt gttttccgaa actggtatat
    24781 gaaatttcac ctttttacga cgtggccgaa ccgccgcgcc ggaggggcct caacgttcag
    24841 gtttgccggg agtggcgcat gatcaggttc caggcggttc acaagtggtt caagaaactg
    24901 catgtgttga atgacgtcaa cctccatgtc aagcagggcg aggtcgtcgt cgtctgcggc
    24961 ccttccggat cggggaaatc caccctcatc cggacgatca acaagctcga acccatccag
    25021 aagggcaagc tgttcgtcga cggacaggac ctgtccgacc ggaaaaccga catcaacaag
    25081 ctgcgggcgg aaatcgggtt cgtcttccag cagttcaatc tctatcccca cctgagcgtc
    25141 atcaaaaaca tcaccctggc gcccatcaag atccgcaagc tcgcccgtaa agaggcggag
    25201 gagcaggcca cggccctgct ggagcgggtc gggctcgcgg agaagcggga tgcctacccg
    25261 gcgcagctct ccgggggaca gcagcagcgg gtggccattg cccgcgggct cgccatgaag
    25321 ccgaagatca tgctcttcga cgaaccgacg tcggccctcg acccggagat gatcggagag
    25381 gtcctggagg tcatgcaggg cctggcccac acgggcatga cgctgatcgt ggtgacgcat
    25441 gaaatgggat tcgcacgcga ggtggcggac cgcgtcacct tcatggatta cggggtgatt
    25501 ctcgaagagg ccacgccgga cgaattcttc aagaatcccc agcacgagcg ggcgcagttg
    25561 ttcctcaagg agatcctctc tccgatgcac taggagagaa aaggcgatgg gctagaggca
    25621 ccgggccata aggatggtcc tgaattcagc cttccatggg aattcattca gtgttgaaag
    25681 gaggacaagc atgatgaagc gattggggat gtggggattg gcactggtgt ttgcgtgcag
    25741 cctgggggga acggcgctgg ccgcggatac cctggcggac gtgaagagca agggcgtgat
    25801 ggtctgcggc gtgaaggatt ccacgcccgg gttcggctac atcgacgagc agacccgcca
    25861 gatcatcggg tacgacgtgg acttctgcaa ggccattgcc aagaagctcg gcgtcaatct
    25921 ggagttgaga cccgtgacgt cggcgacccg gatgccccag ctcatcgccg gtaacatcga
    25981 catcatcgcc gccacgatga ccaagaatgc cgagcgggcc aaacagatcg atttcagtta
    26041 catgtacttc ttcaccggcc agaagttcct cgtgaagaag ggaacggtca agaagctggc
    26101 cgacctcgag gggaagcgga tcggcacggc caaggggtcc acctccgagc agaatgcggc
    26161 caaggcgctg cccaaggcca cgatcctgtc cttcgacgat tatccccagg ccttcctggc
    26221 cctcgagcag gggaaggtgg cggccgtcac gaccgacgag tcgatcctgg cgaacctgct
    26281 ggccaaggcg cccaacaaga ggaaatacga aatccccaac atccagatct ccgacgagcc
    26341 ttacggcctc gggatgcgca agggcgacgt gaatttcgtg aacgccgtca acaaggccct
    26401 cctggacatg gagaagaagg gcgaggcgaa gaaaatcttc agcaagtggt tcggttccaa
    26461 ggggaagtcg gggatcgtcc tgaagcggac cttcacgatt acgccggaac gataagagga
    26521 gcttcctcca aacgtgttca actatcagtt cgactgggca atcgttacat cagggcagta
    26581 ttttgaatgg atcctttccg gcgtcaaggt caccattcag ctgtccctgg tgtcgattgc
    26641 cctcgctttt atcctcggcc tgctgatcgc cgtcatgcgg atgagccaga acgcgcccgt
    26701 ccgctggttg gcgatgggct acctcgagtt tttccgcaac accccgctcc tcgttcagat
    26761 cttcttctgg tatttcggct ccgacaagat cctcccgcag gccgcgcggg actggctgta
    26821 caccatgaat ttcgaattcg ccgccgcggt catcgcgctg acgatctaca cctcggcctt
    26881 catcgccgag gacatccgct ccggtgtccg ctccatcccg aaggagcaga tggaggcggc
    26941 ccgcagctcc ggattctcct atctccgatc catgctgtac atcatcctcc cccaggccgt
    27001 gcggatcacg atcccgccgc ttatcaacca gttcctcaac ctggcgaaaa actcgtcgct
    27061 cgccatgacc atcggcgtgg cggaactgac gtatcaggcc cggcaggtgg aaagctacac
    27121 gttcaagggg ttcgaggcct ttacggcggc gaccctggtc tatctggtga tctcgctcgt
    27181 catcacgggg ttgatgaccc tctacagcaa gaaggtgctg agccccatcg ggatgcgata
    27241 acgtatggaa accggactcg acttcaccgt catctggacg aacatcaccc acttcatgat
    27301 cgggcgctac ccgagcggcc ccatgggggg ggtgttgctc acgctttacc tggcggcggt
    27361 ctcgctcgtc ctgtccttct tcggcggtct gattctgggg ctcatgtgcg tctcgcgcaa
    27421 cccgctcgtc cgctatccca ccctggcggt ggtgaacacg atccgcggga tcccgctcct
    27481 tttggtgatt ttctggatgt atttcctgct gcccactttc ctgggctacc ggacgccgga
    27541 gagctggacg gtcatcgcgg cgctgacgat cttcacctcc gcctacatgt cccagatcgt
    27601 ccaggccggg atcgagggca tccccagcgg tcagatggag gcggccctct cgacgggact
    27661 ccacccgcgc caggccatgc tgttcatcgt cctgccccag gccctgcgta acatgatccc
    27721 ctccttcgta aaccagttcg tctccatgat caaggacacg tcgctggcgt acatcgtcgg
    27781 ggtttccgaa ctgacgcacg tggcgaccca gatcaacaac cggacgatga tctaccccac
    27841 ggaaatcttc ctgttcatcg ccgtgatcta cttcatcatc tgctttgcgt tcaccagcct
    27901 gtcacgctgg ctggagaaac ggctcgcctg gcgcaaggcg atctgaccgg ctcccggccg
    27961 cacccgcccc tttttttctc gaaaatcaat gcggattatt ttttggacag gacgctctcg
    28021 aagaaggccc gcttcttgcg cggcaccaga acggcaccct cgtggtccac gatcaggatg
    28081 ccctccaggt ggtccatctc gtgctggacg acgcgtgcgc cgaagccggt gtagcgcttg
    28141 ttgatcttgc gcccttcccg gtcgtaggcg cgcaccttga tttccgggtg gcgttcgatc
    28201 tcgacctgga tctcgggaca ggacaggcag ccctcggcgc cccggacggg ttctccccgc
    28261 gtctgggtga tgcgggggtt gacgagaacg tcgtaatcct tgtcgctggc gggccggccc
    28321 ttttcatcga aatgtttgtt cttgaaaatg atgacccggc ggccgatccc gatttggggg
    28381 gcggccagcc ccagggcgtc gtcccgctcc agaaaggcgt ccatcagggc gcggatgtcc
    28441 tcccgggcgg ccctgttcag ggggatgggg agctcctcac aggattcccg caggcgtttc
    28501 gcctccgctt cgttccagtg atcgtccgac cagagggtca ggatttttcc ttccgtcatg
    28561 ggatttctcc aagtttttcg tgcacccggc cgccctatac cacccttggg gacggaattc
    28621 aagggaggcg ggaccgcgcg aatccgcttg atcatcggga ggatgtctgc tacgtatcgg
    28681 gggcggcctt cacctcccct tcccccgcgg gacggggcgg ccttcgccgc cggggaccct
    28741 tgcggccccg cgtgagcacg cagccttcag gcatccggaa cggcgcactg cgccgttccg
    28801 gagagcggat cgatcatgac cttgccgttt gtcccgtccc tcattacggc cgtcgcccga
    28861 cgggaattct cccgggccat gcatttcgag ccgggccggc cggtcgactt cccccccggg
    28921 gaccggtccg ctccccgtct gctgtacctc catgtcccct tctgcgagca gctctgcccc
    28981 tactgttcct tcaaccgggt ggtgttcgaa ccggagctgt gccgggcgta ctttcgcgcc
    29041 ctgcggcggg agatccgcct ctatgccgag cgcggctacg acttcggcgg tgtctacgtc
    29101 ggcggcggga cgccgacggt cctgccgggc gagctgggcg aaacgctgga cctggtccgc
    29161 gcgtccttcg gcattcgtga aatatcggtg gaaacgaatc cgaaccacct gacggacgag
    29221 accctggcgc tcctgaagga cgccggggtg aaccgcctgt ccgtgggggt acagagtttt
    29281 caggatccgc tcctgaggca gatggagcgc tacgcgaagt acggcagcgg cgaggccatc
    29341 acccgccgcc tgggtcagat cgcggggcgt ttcgatacgc taaacgcgga catgatcttc
    29401 aactttccct cccagacgcc ggagatgctc gcggaggacc tcgccgtgat ccggtcgctt
    29461 ggggtggatc aggtcacctg gtacccgctg atggtctccg attccacgcg ccggaaggtg
    29521 agccggaccc tggggcgtgt ccgttacagc cgggaaaggc gctattacgg tgaaatcgtg
    29581 aagggccttc tgccgatgta ccgcctttct tcggcctggt gtttctcccg ccagccgtcc
    29641 ctgatcgacg agtacatcgt cgattacgcg gagtacgccg gtctaggcag cggttccatc
    29701 ggctatctgg acgggacctg ctacgccaac accttccgga ttcccgacta tatcgcgcag
    29761 gtcgaacggg gcgagatccc cctgctcgcc gcccgggcct tttcccggcg tgaccggatc
    29821 cggtatgact ttctcatgca gctgttcggg atgaccctgg atatccgggg gctcaagaag
    29881 aagtatggac cggcgatctg gcggcatctc tgtttccccc tgctcgcctt cggcgcggcc
    29941 ggcgggttgc ggtaccgcgc cccggtcgtc cgcctgacgg cgaccgggcg ttacctgtgg
    30001 gtgatcatga tgcgagagtt tttcatcgcc gtgaacaact tccgtgacga ctgccggaac
    30061 ggggacggac cggtctgagg ggccggtccg tattatccgc atgtttgcag gttccggaga
    30121 ccttgtcctc cgttcggtcg gcggagaggt tgaggaattc ttatgaagcc atgagttggc
    30181 gctttcgggg acggtggcgg aatggatgaa cggaaaaaga cgttgttgcg ggagctgccc
    30241 aagatcgatg cgctgatgct cctgctcgaa gcgaagggtg cgaccgcact ggccccgcgg
    30301 gaactgattc ttcagacgtg ccgggcggtg gtggccgatc tgcgggcgga gatcgtgggc
    30361 gtcgaagggc cgggcaagac gtcgtccggg aaacgcggcg gcgatttgcg ggtcggaccg
    30421 gagtccctgc gcctgccgac gccggaggag gtcgccgccc gggtcgcggc gcgcctcgag
    30481 gacctccacc gctaccgcct ccgccgggtg atcaacgcga ccggcgtcat cctccacacg
    30541 aatttgggac gggcgcccct gtgccccgag gccgtcgacc ggatcgtcgc ggtcagccgg
    30601 ggctacagca atctggaatt cgacctggag aggggtgagc gggggcttcg ctacgatcac
    30661 gtccgggacc ggctgtgtgc cctgacgggc gccgaggacg ccctcgtcgt caacaacaac
    30721 gcggcggcgg ttctgctggt cctgaacacc ctggctttcg gccgggaggc catcgtctcc
    30781 cgcggggagc tcatcgagat cggcggggaa ttccggattc ccgacgtcat ggagaagagc
    30841 ggcggccggc tccgggaggt gggcacgacg aaccgcaccc gcctggccga ctacgaacgg
    30901 gccctcgggc cggagacggc cctgatcctc aaggtccaca cgagcaactt ccggatcgtc
    30961 ggcttcacgg aagaggcgga cctgggcgaa cttgtcgccc tcggaaaacg atccggtgtg
    31021 cccgtcctgg acgatctcgg gagcggttgc ctgatcgggc tcgagcatta cggcctcatg
    31081 cgcgagccga cggtccagga cgtcctcgcg acgggagtcg acgtcgtcac gttcagcggg
    31141 gacaagctgc tgggcggtcc gcaggcgggg attatcctcg ggggaaggga gattttgggg
    31201 cgggtcaaga agaaccccct gaaccgggcg ctccggatcg acaaactgac cctggcggcc
    31261 ctggaggcga cgctcatcca ctatctcgat ccggaagcgg cccgtgagcg gctgcaggtc
    31321 ctcaaggccc tgacggagcc cctgccggac gtgaagaaac gggcgcatcg cctgctccgg
    31381 cttctgcgcc gggcggggct ggccggcctg gacctctccc tcgaggcggg ggtgtccatg
    31441 accggcggcg gctcgctgcc gacccaggag atcccgacgt cgctcgtcgc cctgaggccc
    31501 cggtctctgt ccgccgcccg cctggaggag gggctgaggc gtctgccgac gccggtagtc
    31561 gcccggatcg ccgaagacgc ggttctccta gacgtgcgga ccctgatgga ggaggaactg
    31621 gggtgtatcc gcgacggctt gagaaccctt ttccccgttc ggccgcacga tccgggaagg
    31681 aacccctgac ccgtcacagg cgtggccgcc gtcgaaaccg tcccggatgg gcggcggccc
    31741 gccgggaccc gtgttatgga taaaaccatc gagacctgga ccgtttcccc gtccctggac
    31801 ggcgcccgtc tggacctgtt cctgacggag gcgtcgtcgc tgtcccgctc ccaggtcaag
    31861 cgcctcgtcg aggggggccg ggtctcgatc gacggcgtcg tcgctccgaa ggtcagccgg
    31921 aagctgaagg cctgcgagac cgtcgtcctg aaccgacccc cggtcgccgt gatggaagcc
    31981 ctcccggagg acattcccct gtccattctt tatgaggatg cgcacctgct ggttctggac
    32041 aagccggcgg gcctcgtggt ccacccggcg cccgggcatg ccgggggcac ccttgtgaac
    32101 gccgtcctcc gccattgccg ggatctttcc gggatcggcg gggtcctgcg ccccggaatc
    32161 gtccaccgcc tggacaagga cacgtccggg gtcctcgttg tcgccaagtc ggacacggcc
    32221 cacgcggggc tggtcaaccg gttcaagcga cgggagatcg gcaagaccta cctcgcgctg
    32281 gtctggggcg cgccccggga aaacgcggga cgcatcgacc tgcccgtcgg ccgccatccg
    32341 cgggaacgcc agcggatgtc cgtccggggc cgtcggggca agccggcggt gacgcactgg
    32401 cgggtggtcc gtcgctacgg ggccgtgagt ctcctcgagg tggccctcga gaccggccgg
    32461 acccatcaga tccgggtgca tctgaacgcc attggccatc ccgtcgttgg ggaccccgtc
    32521 tacggcggcg ccggaagggt ccggggcgtg gccgatccgg tcctccggtt ccggctccag
    32581 gcgatgaagc gccaggccct ccatgcggcc cgcctcgatt tcgtccaccc cgtgaccggg
    32641 atgcccctga gcgtgagcgc accgcttccg ccggacatgg cggagctgac ggcgtttctg
    32701 gaaaaggatg ccggaggcgc cgcgcccgac ctgggcaacg gcgccccgcg tccgggggga
    32761 gcgcgatgag acccgcgcgg gaggcacggg ccggcgggcc ggcgcgctcg ggcgcatccg
    32821 gggaaggctt ccggattcgc cgccgcggtg gcctgagggt gttcgaagcc gatctcctgg
    32881 cggagatccc ttccctccgg cacgccttct ccacccgccg gggcgggagc agcggcgggt
    32941 gcttctccag cctcaatttc agcttccgcc agggggacga ggcggaaaac gtccgggcga
    33001 actgggcggc cctgtcccgg gccttcggga tcgcgccgga gcgcttcctg accgtccacc
    33061 aggtgcacgg caaccgggtc tgggtgatcg acggagcgga atcgattccg acgtccggcg
    33121 atggagacgc ccgggaagac gcgccccgct gtgatgcgat cgtcacaaac cggccgggga
    33181 tcgccattgg cgtcaagacg gcggattgcg ttccggtcct gatcgcggac ccggtccggc
    33241 gggtgatcgc ggccgtccat gccggctggc aggggacggc ccggcgcatc gtggaacatg
    33301 tctgcgccgc gctcgaggcg cggttttcct gccggaggga ggatctccgc gccgccatcg
    33361 gcccggccat cggcccgtgc tgttacgagg tggacgaccg cgtccgggag gcgatggccg
    33421 gtcttcccgg cctggaacgg attttctctc cccgggatgc gccgggacgt tggcggctcg
    33481 atctcgccct ggccaaccgg ttgcagctcc gaaatgcggg cgtgccggcg tcccgggtgg
    33541 cggaggcgaa cctgtgcacc gcctgccggc cggacctctt cttctcgcac cggggcagcg
    33601 gcggaaaagc cgggaggcac ttcagttttc tcctgatgga cggggagtcg ggcgccgggt
    33661 gaagggtccg ggctcttcgg aaaggggccc ttcccgcggg aacgggaaac cggcggcgaa
    33721 acggtgtccg ggctgttcgc cgggtcttga ggagcggaca tgaggacgcc tgaaaaaaat
    33781 cttgacacga acgggggcat ggggtaaggg aagctcaact ctgaacggtt ggtgcgaagt
    33841 ccttccccca tggaataacc ccagaattcg cgttagcaac ccggttccat cccagatctt
    33901 ccatatgatt tcgttgcaat gagaatgatg gccgccgcgt cgctgtgttt cagcgcgtgc
    33961 gtcggtgccg gaaacaggga gaaaagaccg tccttacggg aagagcaatt tgacaatgaa
    34021 gaaggaatct gtatgaatat cgatgaaatc aagcgtttgc cgatcagcca gttaaccgac
    34081 atggccaagg acttcaacgt ccccggggcc ggggggctca ggcgtcagga cctcattttc
    34141 gccatcctcc aggccgaagc ggagaagaac ggcgtcatct acggggcggg cgttctcgag
    34201 atcctgcccg acggcttcgg gtttctccgg gccgtggact acaattatct gcccagcccc
    34261 gacgatatct acatctcccc ctcgcagatc cgccgcttca gcctccgcac gggtgatacg
    34321 atctccggcg aggtccggcc gcccaaggag ggggagaagt attttgccct cctcaaggtg
    34381 gatacggtca atttcgagag ccccgagacc gcgcgcgaca agatcctgtt cgacaacctg
    34441 acgcccctct acccccagga aaagctgaac ctggaatcga atccggagaa tttctccacc
    34501 cgcgtcatgg acctgttcac gccgatcggc aagggacagc gcggcctgat cgtttcgccc
    34561 ccgcgggccg gcaagacggt gctcctgcag gacatcgccc acagcatttc cgccaatcac
    34621 aaggaagtca tcctgatggt gctcctgatc gacgagcggc ccgaagaggt gacggacatg
    34681 cagcggagtg tccagggaga ggtcatctct tccaccttcg acgaacccgc ctcgcgccac
    34741 gtccaggtcg ccgagatggt catcgagaag gccaagcgcc tggtcgagca caagaaggac
    34801 gtcgtgattc tcctggacag catcacccgg ctggcccggg cctacaacac cgtcgtcccc
    34861 cccagcggca aggttctctc cggcggcgtc gactccaacg ccctgcacaa gccgaagcgc
    34921 ttcttcgggg cggcgcgcaa catcgagaac ggcggcagcc tgaccatcat ctccacggcc
    34981 ctgatcgaca cggggagccg gatggacgag gtcatcttcg aggagttcaa gggcacgggc
    35041 aacatggagc tgcatctgga ccgcaggatc gccgaccggc gcgtctttcc ggccttcgac
    35101 ttgattcgtt ccggaacgcg gaaggaagag cttctgaccc cgaaggccaa cctgaaccgg
    35161 gtctggatct tgaggcgcct cctccaggag atgaaccccg tcgatgcgat cgaattcatc
    35221 gtggacaagg tccgcaagac ccagtcgaac caggaatttc tggattccat gaaccagtaa
    35281 gggccaagac gacaaaaaga cttgtttttt ttttcgagat ggctataatg caccccctgt
    35341 gcacgcgcgg ggcggcgaga cggtccgccc cggggcgggg acaatcggga aggaggctgg
    35401 cgaacagcat gaaggaaggg atccatccag actatcagga aacgaccatt acctgcgttt
    35461 gcgggaatgt catcgaaacg cggtccacga agaaggacat caagatcgaa atttgctccc
    35521 agtgccatcc gttcatgacg ggcaagcaga agatcgtcga cacggaaggc cgcgtggaac
    35581 ggttcaagaa gaaatacgcc ggcacgggcg ggaagaaata atccgctgcc ccgtgcgggg
    35641 gccgaaacgt ccctccgttg cccccgatcg gaacgaatct caggaaaggc gcggtgaagg
    35701 cgcattgtta cggccctatc cggtgtgcac cctttcgacg acacctccac cccgcccgga
    35761 cgatcccggg cgggcggttt cccttgaccc cggcgctgca ttccattctg acatttttcc
    35821 ttcggcgtgc ccggaagcgt ccgcgctccg gactcgccgg atagggcggg cgttcatgcg
    35881 ggaccggttc aggccggtgc cgtccggacg cgccgcgggg aacgaccatg ttcaaacgga
    35941 tcaaggagat tgaaagccgc tacgaggagc tcgagcgtct gctgagcgat ccggaggtca
    36001 tcgcgaaaca ggccgtttac cagcgccacg ccaaggagca tgcggatctg cgcgaactcg
    36061 tggagacctt ccgcctctac gagaaaacgg aggcccggat ccgtgaggat caggagcttc
    36121 tccgggggcc ggatgaggac atccgcgaga tggcgaagga ggagctcccc atcctgaagg
    36181 aggccctcga cggactggaa gagcgcctga agcggctcct gctgccgaag gaccccaacg
    36241 acgagaggaa cgtcttcctc gaaatccggg ccgggaccgg cggcgacgag gcggggcttt
    36301 tcgccggcga tctcttccgg atgtacgcgc ggttcgccga gcgcaaccgc tggaagatcg
    36361 aggtcgtcag cagcaccccg gccggcggca tgggcggttt caaggagatc atcgccctca
    36421 tcacgggggc gggcgccttc agccgaatga agtacgagag cggggtccac cgggtccagc
    36481 gcgtgcccgt caccgaggcc caggggagga tccacacgtc ggccgtcacg gtggccatcc
    36541 tgccggaggc ggaggaggtg gaggttcaga tcgatcccaa cgacctgagg atcgacgtgt
    36601 accattccag cggtcacggc gggcagagcg tcaacaccac ggattccgcc gtccggatca
    36661 cccacctgcc gacgggactc gtcgtcacct gccaggatga gaaatcccag ctcaagaaca
    36721 aggccaaggc gatgaaggtt ctccgcgcac ggctgctgga cggcatggtg acccggcaga
    36781 acgcggagat ctcggaggcc cggaagatca tggtgggcag cggtgaccgg agcgagcgca
    36841 tccggaccta caattacccg caggggcggg tgtcggatca ccggatcggc ctgaccctct
    36901 acaacctgga gggctttctg gacggggaca tcgaggaggt cctcgaggcc ttgagcaccc
    36961 attaccagac ggaagccctc aaacagtccg ccggctgacg aacatgaaca ttcgcggact
    37021 catcgaccgg accacccgga ccttcgcgcg ggaaggcctc ccgacgcccc gcttggatgc
    37081 cgaggtgctc ctcgccgcct gtctgcggac ggaccgaagc gaattttaca ttcatcccga
    37141 gcgtgaggtg ccgcccgatc accgccgccg ctttgccagc tgggtcggcc gccgccgccg
    37201 ggggcagccg gtggcctata tcacgggcga gaaggaattc tggtcgctgc cgctcacggt
    37261 gggcccgcag gtcctcgtcc cgcggccgga gacggagatt ctggtggaag aggtgctgcg
    37321 cctggcttcc tccctgccgg ccgattcgag gattctggag gtcggcgtcg gcagcggcgc
    37381 cgtcagcatc gccttggcga cggagctgcc gctcgcccgg atcgcggcca cggacatttc
    37441 cccggacgcc ctcgcgctgg cgcgcgtcaa cgcgcggcgc cacggcgtcg atgaccgtat
    37501 cgccctgatg gcgggggatc tgttcgaacc ggtgcggggg ccgttcgacc tcgttgtctc
    37561 caatccgccc tacatcccgg agggggcgta cgacggcctg ccggcggagg tccgctgcga
    37621 accgcggctg gccctgctcg ccgggccgga cgggacggac gtgcaccgcc gcctgatcgc
    37681 ggacggcggg cggtacctga cggcgggcgg ctggctctgc ttggagatgg gcgagggaca
    37741 gggcgggcgt ctgaccgccg tcctggagaa ggcgggatat gcggacatcg tccggaggaa
    37801 agactacggc ggcagggaac gggtcatccg ggcccgatgg aggtgagggt tggataagat
    37861 cgtcatcgaa gggggccgca ggctcgaagg ggaggtccgg atcagcgggg cgaagaacgc
    37921 cgccctgccc atcctcatct ccgcgctcct gacggaaggc ccgaacacct ttcacaacgt
    37981 tccggacctc gtggacatca agacggtcaa gaaattgctg gcgagcttcg gcgtgcggat
    38041 ggaggggaga gaaacgcttt cactcgatgc gaccgacctc acccgttgcg aggcctccta
    38101 cgacctggtt cgaaccatgc gggcgtcgat cctcgtcctg gggccgctgg tcgcgcgtct
    38161 gggcgaagcc cgggtgtctc tgccgggcgg atgcgccatc ggggcccgtc cggtgaatct
    38221 gcatatcaag gcactccagg agatgggcgc ggaggtcgaa ctccgggacg gctacgtgga
    38281 ggcccgttgc ccccgcctga acggggcgga gatctacttc gacatctcca cggtgacggg
    38341 aacggaaaac atcctgatgg cggcctgcct ggcccgggga acgacggttt tgaaaaacgc
    38401 cgcccgggaa ccggagatcg tgaatctggc gcaggtcctg acgggcatgg gcgcccggat
    38461 cgacggggcg ggaacggacg agatccgcat cgagggggtg gagcggcttc atcccgtcga
    38521 tgcggcggtg atcccggacc ggatcgaggc gggcacgttc atgatcgccg ccggcatgac
    38581 cgggggcgac atccgcctga ccggttgcga tccgacgcac ctggatgccc tgatcacgaa
    38641 gctgcgcgaa acgggcatgt ccatcgcggt cgggggaggc gaaatccggg cggccggcgg
    38701 aagccggatc aagagcgtcg acatcacgac cctgccatac ccgggatttc ccaccgacct
    38761 gcaggcgcag atgatggcct acatgacggc cgcctcggga ctcagcgtca tcacggaaac
    38821 cgttttcgag aaccgtttca tgcacgtgag cgaactgctc cgcatggggg cggacatcca
    38881 gatccagggc aacagcgcgg tggtcagggg gacgcccgga ctccggggtg ccgcggtgat
    38941 ggccacggac ctgcgggcct cggcctccct catcctggcc ggactcatgg ccgagggccg
    39001 gacggagctc ctgcgggtct accacatcga tcgcggctat cagcagatcg aaaagaaatt
    39061 ctccgcgctc ggggcggata tcaggaggat gcgggcttga catgaagatc agacgaacat
    39121 ccgagcggga tttcgacgcc ttttttcgcg atctcagact gcggggccgt gccttcgacg
    39181 agactctgct cgcggaagtg aaggggatcg tggcggatgt ggcctcccgc ggggatgacg
    39241 ccctgttcgc cctgacggcg cggttcgacg ggtacacgcc gacggcggaa accgtcgagg
    39301 tcgcgccgac ggagtggtcc gccgccgcgg cgggggttgc ggcggcggac ttcgaactga
    39361 tcgaactggc ggcccgccgg atcgaacggt ttcaccggca gcaacggatc gacagctact
    39421 gcgcggaaga cgaaccggga atcacctggg gacagcgcat cctgccactg gagcggatcg
    39481 ggatctacgc ccccggggga ctggccgcgt atccctcgac ggtcctcatg gcggccatcc
    39541 cggcccggat cgcgggtgtg cgggagatcc tcctcgtgtc tcccgcccgg gaggggaggc
    39601 tcaaccccct gatcgccgcc gccgcgcggg tggccggcgt gacgcgcatc ttcaagatcg
    39661 gcggcgcgca ggccatcgcc gccctggcct acgggaccgc ctcgatcccg gcggtggaca
    39721 agatcgtggg gcccggcaac gcctacgtgg cggcggcgaa gagggtggtg tacggccagt
    39781 gcgccatcga catgatcgcg ggccccagcg aagtcctcat catcgccgac ggatcgcaga
    39841 atcccgccgt ctgcgccgcg gacctcctgt cgcaggcgga gcacgacccg ctcgcgtccg
    39901 cgctgctgtt gacgccggac gatgcgtacg cccggcgggt gacggcggag gtcgaacggc
    39961 agctcccgac cctgtcgcgc cgcgacatcg ccgcccgctc ctggtcggat tacggcgcgg
    40021 cggtcgtcac gaaagacctc gcggaggccg ttgacctggc caaccgcttc gcgcccgagc
    40081 acctggagct gatggtggcc gatccggagt cgctcctgcc ggggatcgtc aacgccggcg
    40141 ccgtttttat gggcggctgc acccccgagg ccgtcggcga ctacctggca ggtccgaacc
    40201 acatcctgcc aacggggggg acggcccgct tcgcgtcgcc cctgggggtg tacgactatc
    40261 tcaagcggat gagcgtcctg tccttctccc gggaggctct gatgaagtac ggccgccagg
    40321 ccgcccggct ggcggacctc gaggggttgg agggccacgg aaaatccatt acccggcgaa
    40381 tggacgcgga tccagagaat ccttgagcgg acgatgaaaa ccgaacttta ctactacagc
    40441 ggtacgggca attccctctg gaccgcccgc ctgctcgcgc aggcgctggg atccgccgcg
    40501 gtccggccga tatcccgtat ggacggttcc accgtgacgg ccgggatcga ggcggtcggc
    40561 ctcgtcttcc ccgtgcatat ctgggggccg ccgcgccggg tgatcgcctt cgcgaatgcc
    40621 ctcaacgcgg acccggcgac gtattgcttc gcggtggcgg tcaacgccgg gcaggtcgcc
    40681 gccaccctcg tccaactgaa aaggattctg catgcaagaa acctgaccct ggcctccggg
    40741 ttcggcatcg tcatgccctc caactacatc ccctggggcg ggcccggccc ggaggaggaa
    40801 cagcgccggc ggatcgccgc tgcccgggag aagatcggcc gcatcgccgc ccgggtcggg
    40861 aaccgggaat cgcgacccgt cgaaaagggc ccctggtggc agaacgtcgt cctgaccgcg
    40921 ctcaaccggc tcgcttttcc ccacgtcccc gcgatggaca agggcttccg ggtcgatgaa
    40981 acatgcaacg gttgcggcgt ctgcaagacc gtctgcccgt gcgggaacat tgcctgggag
    41041 gcggaacggc cggtctggca gcaccgctgc gagcagtgtc tggcctgcat tcagtggtgc
    41101 ccgcgggagg cgatccagta cggcaggaaa acgccggggt acgcgcgcta ccaccacccg
    41161 gagatcaggc tgcgcgacct gacccgggag agggatttgc ggggctgacg ccgattggag
    41221 gtgacgcgat gagaaagatc gtctttgcgg ggttgggggg ggtgctcctg tgcgcgaccg
    41281 ttgcgtcggc gacgggtccg cacttccgga ccgatgcgtt ccgaacctcc gccggggagc
    41341 tcgtcatcac cgtcgtgggt cacgcaacgc tgatgatggc ctggggcggc aaggtcctgc
    41401 acatcgatcc ggtgaccggg caggccgatt atacgaagat gccgaaggcc gatctgatcc
    41461 tcgtcaccca cgagcacgcc gatcatctcg accccgcggc gatcggcctg atccggacgc
    41521 aaaggaccgc ggttctcgcc tcggcggagg ccgcccgcag gatcccgggc gccgtcgtcc
    41581 tgaagaacgg cgacgtccgc acggtccaag gggtgcggat cgaggccgtc ccggcctata
    41641 acctcgtcca catgcgcaag tccggcgtgc ccttccaccc ccgggggatc ggcaacgggt
    41701 acatcgtcac cttcggggac acgcgcgtct acatcgcggg agacacggaa aacaccccgg
    41761 agatgaagcg gctgaagggg atcgatatcg ccttcctgcc catgaacctg ccctatacca
    41821 tgacgcccga aatggtcgcg gacgccgcaa aggctttccg tccgaagatc ctctacctat
    41881 atcacaccag cgggacggac acgggacgtc ttgtcaacct gctccgggat gcgggcggca
    41941 tcgaagttcg gacaaaaaac ccttgacaaa acattccggg tggtacatat gccgtccagt
    42001 cgcggcagac cccgagcggg cgtaattcag tggtagaatg tcagcttccc aagctggacg
    42061 tcgtgggttc gagtcccatc gcccgctcca ttttccccct cccgtcaccg gtttttttct
    42121 tgcaatcccc tcttttctgt gttagggacc cttccagatc atcgggtttt tttagatgag
    42181 tgggcgcgtg cccacttttt ttttcccgaa agaggatcat ggcggagaca tcaggcagct
    42241 accggggaga gatcgcgtca ctcgcggacg tccttctgcg ggccgagggc atggaactgg
    42301 tcgacgtgga atgcctcagg atgaagtcgc gctggctggt tcgaatcttc atggacaagc
    42361 cgggcggggt gaccgttgac gactgtgcct tgatcagcga gcagttgggg gatgtcctcg
    42421 atgtgcgcga cctgcctccc ggggcctata ccctggaggt ttcttcgccc ggtctggacc
    42481 ggcccctggc gaaggcggag gattttctcc ggttccgggg ctcccggatc gactgccgtt
    42541 tgaaggagaa ggtcgcgggg agtcgtcatc tgcgcgggat cctgcgcgac tgccttttgg
    42601 gggagggtcc cggggtcttg gtgatcgagg tggacggtga acgccggtcg atttcccggg
    42661 atctgatcct gaaggcccgc ctcatctatg aagaggaacc ttaggaaaaa gcgtatgcgc
    42721 cgcatcgccc catggggcgc caggggagat aaagggagga caaggatatg tttccagaac
    42781 tcaaacgtct gatcgagcag atgggcaagg accgggggat cgacaagcag atcatcgtcg
    42841 aagccctgga ggtggcgatg gagacggcgg cccggaagaa gatggggccg cgggtggaga
    42901 tcgaagccca ttacaacgac gaggccggcg agatcgaggt gtttcagttc aagaccgtca
    42961 tggagagggt cctggatccg gagatccaga tttccatcga ggaggcccgg gagaagtggg
    43021 acgaaggcgc cgagccgggc gacagcctcg ggatgaagat cgatgccggc accttcgggc
    43081 ggattgccgt ccagacggcg aagcagatca tcatccagcg cgtcaaggac gcggagcggg
    43141 acaacatcta cgaggagtac aaggatcgca agggggaact ggtcaacggg ttcgtgcagc
    43201 gtttcgaggg agggagcatc atcgtgaacc tggggcgggc cgaggccgtg gtgccggtgt
    43261 cggagcagat ctttcgggag cagtacaagc ggggcgagcg gatccgcggg tacctctgcg
    43321 acgtcaagaa gatctccaag ggaccgcagg ttatcctctc ccggacccat ccggggtttc
    43381 tgaaggccct cttcgatgtc gaggttccgg agatctccga ggggctcatc gagatcgtca
    43441 acgtttcccg cgagccggga aagcgggcca agatctccgt gaggacgaag gacaaggaca
    43501 tcgatcccgt cggagcctgc gtcggcatgc gggggtcccg cgtccagagc gtcgttcagg
    43561 aattgcgcgg cgagaagatc gacatcgttc cctattccga cgatccggcc aagtacgtct
    43621 gcagcgccct gtcgccggcg aaggtggaca aggtgttcat cgacgaggac aaccgggcca
    43681 tggaagtcat tgtccccgat gaccagttgt ccctggccat cgggaaaaac ggtcacaacg
    43741 tccggctggc ggtccgcctg accggctgga agatcgatgt caagagccaa tccatggcga
    43801 cggcccagga tgaagagggg ttcaagtcgc tcatgaagat tccgggggtc ggcgaggcga
    43861 cggctgagcg gctgttcaag gaaggcctga agagcgtcat ggcgatcgcc gcctccgacg
    43921 tcgagaccct gtcggcgatt ccgggcgtcg gcgagaagtc ggcccggcag tggatcgaag
    43981 ccgcggggaa gatcctcgat caaggggtcg cgtaaacgga taaggcgggg gagttgcgca
    44041 tgtctaaaaa aagggtccat gaattagcga aggagctggg catcgagaac aaggagttga
    44101 ttgcccgcct ggaaaagatc ggcattgcgg tcaaatccca ctccagcacg ctggatgaag
    44161 gcgatgtgga gcgtattctc aaggagctca aatccagcga accgcgggag gttgtggagc
    44221 agaggatcaa aacgggggtg atccgacgcc ggtcggtacg tctaccggcg gaagaggccg
    44281 agcctgcacc cgccacggag gcgccggcgg aagccgagaa gtccgccgcg gaagcgcttc
    44341 ccaaagaacc ggtgaagccg tccacggccc cgccggccgt acctccggcg gaagccgttc
    44401 agcccaaagg ggccgcgccg aaaagggcgc ccgtttcgaa ggccgccccg ccggctcccc
    44461 cggaaccccc gactgccggg gcggaggtgc ggaaggtcga ggcggccgct ccggcggagg
    44521 ccgcggtcgc tcccgaaccg acgccgaaag agaaggatat gacgccgaaa ccggtgccgg
    44581 tcgcccggga gaccgagacg gtgtccccca cagcgccggt gcctccggcg aaggccgttc
    44641 catccccgtc ggccaggacc gccgaaaagg aagttcgaaa gcccgcccct tctcggccgg
    44701 acctgatgat cacccgggat gccgttcctc cgcggccgga agcgccgtcg gcgcggacgg
    44761 aggccgacaa gttcaaaaaa cgcgggaaga aaccggtcga agttctgatg gaagaatcgg
    44821 cgccggccaa gaagaaggtt ttcgccaaga agcagttcac cgaccgtaag gcgcgacaga
    44881 tcgaggtcga tacggaggaa cggacgccca gatggcggga ggagaagcgt cccgccgttg
    44941 tcaggatgaa gaagacggaa atcacggttc ccaaggccag caagaggagg atcaaggtgg
    45001 gcgatgccat ttcggtgggg gatctggcca agaagatagg cgtcaaggcc agcgaggtca
    45061 tcaacaagct catgggattg gggatgatgg ccacgatcaa ccagtccatc gatttcgagg
    45121 cggcgagcct gatcgccacg gagttcggtt atcaggtgga gccgatgggc ctggaattcg
    45181 acgagaccct gctgaaggcg gagacgcccg cggagaatct ccgaccgcgc gcaccggtgg
    45241 ttaccatcat ggggcacgtg gatcacggca agacgtcgct gctcgacgcc attcgtgaaa
    45301 ccaatatcat cggcggcgag gcgggcggca tcacgcaggc tatcggcgcc taccacgttc
    45361 acatcaagaa ccgggacatt gtgtttctcg atacgccggg gcacgaggcg ttcacggcca
    45421 tgcgcgcccg cggggcccag gtgacggaca tcgtcgttct cgtggtggcg gctgatgacg
    45481 gggtgatgga ccagacggtg gaggccatca accattcccg cgacgccggc gttcccatcg
    45541 tggtggcgat caacaagatc gacaagcccg gggcggattc cggtcggatc aagcaggccc
    45601 tgacggaata caatctggtc ccggaggagt ggggcggaga gacgatcttc tccgaggtgt
    45661 cggccaagca gaagatcggg atcgaagaac tgctggagtt gatcctcctg caggccgata
    45721 tcctggaact caaggccgat cccgaccgcc cggcccgcgg tgtcatcatc gaggccaagc
    45781 tggaccgcgg acgcggaccc gtggcgacgg tcctgattca ggaggggacc ctgcgggagg
    45841 gtgacgcctt cgtctcgcgg acggaatacg gccgggtccg ggccatgatc gacgacaagg
    45901 ggaagcggat ccgggaagcg gggccggcca cccccgtcga agtgatcggc ttttcccgtg
    45961 tgccccaggc gagctccgaa ttcatcggcg tcgaggacga gaagaaggcc cgcagcatcg
    46021 gcgaatactg gatccgcaag gagcgggagc gggagctttc cgccacggcg aagattaccc
    46081 tggaacagct ctatgcgaaa atcaaggagg gggtgaagga gctcaacgtc atcctcaagg
    46141 ccgacgtgca ggggtccgtc gaggccctcg tggacgcgct cggaaaactg ggcgcggccg
    46201 acatcaagat caagatcatc cattcctcca ccggcgccat cacggagacc gacgtcatgc
    46261 tggcgtcggc atccaacgcc atcatcatcg ggttcaacgt gcgtccggac gcccgggttg
    46321 ccgaggtggc cgaaacggaa ggggtggaga tcaagctcta cgacattatc tacaacgtga
    46381 ttgccgacat gaaggcggcg atggaggggc tcctggagcc ggagtaccgc gaggtcagcc
    46441 agggccgcgc cgaggtgcgg gacatcttca aggtgccgaa gatcggcacc atcgccggct
    46501 gctatgtgac ggacgggaag ctggcccggt cctcgggcgt caagctggtc cgggagggcg
    46561 tcgtcgtgta cgatggacgg atcgcctccc tccggcgctt caaggacgac gtcaaggagg
    46621 tcgcgacggg gttcgagtgc ggcatcggca tcgagaacta caacgacatc cgcatcgggg
    46681 acgtcatcga gtcttacgtc caggaggaaa tcgaacgcaa gctttaacgg gccgacgggc
    46741 ttcggggacg cccgcggacc ggggcgtgag gggggcatgg ttatcggtgt gggactgatc
    46801 gacatcctga tcccgggcag ccgctccctc aaggagaagc ggggcgttct gcggcggatt
    46861 ctcaagcgca cgcagaatga attcaacatc tccatcgccg aggtggggca gcaagacctc
    46921 tggcaggcgg cccggatcgg attctgcgta gtcggaaacg accgcgccta catcaacggc
    46981 aagatggatc acgtttttcg gttcatcgac gacctcggca tggcggagcg ggtccgcacg
    47041 aagatggaaa tcctttcgtt tgcggacatc ggggatgatc cgcgactgga aatggaaaag
    47101 tatggcacaa tataaacggg cggaacgcgt ggcggatctg atccgcgccg aactcgccga
    47161 tatcctgcgc aagcgcatcc gcgatccccg gatcgggttt ctgaccctgt cgggcgtgac
    47221 gctgtcggac gatctcaggg tggccaaggt gtatttcgtc gaaatgggac aggaccagtg
    47281 ccgcccggag atccgggcgg gcctggaccg ggccgtgggc ttcatccgcc gggaactggg
    47341 gaagcggatc gagttgcgct acgtgccgga actcatcttt ctgtacgacc cctccttcgc
    47401 ctacggcagc cggatcgaga ggctgatcca ggaaatccac catcaagaag aaccagagga
    47461 accggatgct ccagaagatc attgacatca tccatcgcgg ccggacgttt ctcattacgg
    47521 cccatgaacg gttggacggg gacgccctcg gatcggagct cgccctgtac gcgatgctga
    47581 ccgatctgcg gaaggaggcg gtggtctaca accaggacga gattcccggg aattaccgct
    47641 ttcttcccgc gagcgaaacc atcgtgtcgg cgctgccccc gccggagacg ttcgacgcgg
    47701 cgttcatcct ggattgcagc gagctggacc gggtgggtcg ggaggcccct cggatcgcag
    47761 gcatccggca actcgtgaat atcgaccatc acgtctccaa cggcggtttc tgcgatacgg
    47821 tcctgctcga tccgaaggca agttcgacgg gggaaatcct ctgccgggtc atgacggcca
    47881 tgaactggga accgacgccg tcggcagcga cgtgtctgta tacggcgatc ctgaccgata
    47941 ccggcgggtt ccattacggc aacacgacgc cggaggcact gacggcggcg gccgggctcg
    48001 tggtgcgcgg ggccgatccg cagaggattt cggaaaacgt ctacgagagc aaccccctcg
    48061 ccaagatccg gcttctgggc gcggtcctgg aaaccctctc cctcgacggg gacgaccgcg
    48121 tgggatcgct caccgtgacc cgcgaggtcc tggcgcggac gggcgcggcg cccgagcata
    48181 cggacggcct cgtggacctg ccccggacca tccggggggt ggacgtttcc gtcctgttca
    48241 gcgagatgga ggacggccgc atcaaggtca gtctgcggtc gaaaggtcgc gtcaacgtgg
    48301 agcgcgtcgc ccgtgccttc ggcggcggcg gtcacgtcaa tgccgccgcc tgtcgagtcg
    48361 agggggatgt ggaagcggtg aaacgaaacg tgatcgcggc ggttgtttcc agcctgggtg
    48421 cggaggagcg ccgatgaacg gcgtgatcgt gatcgacaag ccggcgggga agacctcgtt
    48481 tgacgtggtg gacgaggttc gccgtacgct gggcgtcaga aaggccggtc acaccggcac
    48541 gctcgatccc ctggccacgg gggtgcttcc ggtctgcctc aacgaggcga cgaagctcgt
    48601 gccgttcctc atgggaggat ggaaggtcta tcgggccacc ctgcttctgg gggtccggac
    48661 ggacaccctg gaccgggagg ggcaggtcga gcggacctgc gtcccgtccg cgacgccgga
    48721 ggagatcgcc ggcgcgctgg aaaagtgcgt cggcaggcgc ctgcagcagg cgccccgcta
    48781 ttcggccctc aagttcaagg gcaaggccct gcaccgctgg gcccgtcagg gggtcgcgat
    48841 cgacccgccc tcccggatcg tcgagatcaa cgaactgacg atcgagtcca tcgaggggcc
    48901 ctatgcgacc ttcaccgtcc gctgttcgaa gggaacctac atccgctcgc tctgcgcgga
    48961 ggtcggggaa agcctgggct gcggggcctg tctctacgcc ctgaggcgcc tcaaaaccgg
    49021 cccgttcgac gagaccctgg ccattcgtct ggaagatgag gcgggcgccg cgggaccgga
    49081 acggttgcgg cgggggctga tccccctggt cgacgccctg ccggaattga ccgagatcgg
    49141 ggtcgatgcc tcgctggcga agcggattcg cgccgggcat cagccgaaac ggggcgactg
    49201 gggggaagag gatattcctt ttcttgcccc ggaagatatg ataaaattta cactcaatcg
    49261 gcgtgccttg gtcgccatcg gcaaggtggt tcctcctccg caggcgtcgg gggaggcgtc
    49321 cggcgacgcg ccgtttacca agatcctgag ggtgttcaat ccctgatgag ccgcagcgcc
    49381 gccgtcgaag gcgcgaggcc gaagacgaat gcaagtcaac catgcgccgc attgtgagaa
    49441 ttcggcaaga gaacgacgtt caaggaagga gaaagggcag tgttagatgc ggagaaacga
    49501 aagggcatta tcggtaaata tcagatccac gagaacgata cgggctcccc ggaggttcag
    49561 gttgccttgc tgacggcgcg catcgagtat ttgacggagc acttcaaggc gcacaaaaag
    49621 gaccatcatt cccggcgggg actcttgaaa ctggtcggtc agcggcggcg gcttctcgat
    49681 tacatgaaga aaaaggatgt ggaacgatac cggagcatca tccagcagct ggggatccgg
    49741 aagtaatcct cccgcgggat gcatcgcttg caggaaaacg gatcggcccg gggcctgaat
    49801 ctgtgcccgg gggcctttag agaaaggaat gagaatgagt aaggattttc gttgtgactt
    49861 tgcgggcaga gagatctgcc tgaagacgga ctatgtggcc gcacaagccg atggcgccgt
    49921 gttcgcgcag tacggggata ccgtggtgct ggtgacggcc gtatccctga agacggcgcg
    49981 ggaagggctg gacttcctcc ccctgaccgt ggattatcag gaaaagacgt tcgcggccgg
    50041 caagatcccc ggcggcttct tcaagcggga aggaaaactg aacgagcggg agattctcct
    50101 ctcccggatc atcgaccgtt cgatccgccc gctcatccag aaggggtatt tcttcgagac
    50161 ccagatcgtc gcgacggtgc tctcggtcga cgacgagaac gacagcgacg tcgtggccat
    50221 gctgggggcg tcggccgccc tcacgctgtc gaatatcccc ttccagggac ccatcgccgg
    50281 cgtacgtgtc ggccgcctga acggggcctt catctgcaac ccctcgcatg cccagcaggc
    50341 cgagagcgat ctgaacctct tcctggtagg ccggaaggtc ctgccgggga cggaggggag
    50401 accctatgac gtcaacctcg tcatgcttga aggggaggcc cgggaagtgg acgaggatac
    50461 gctggtcgat gcgatcaact tcggcctgga gtccctgcgg cccgtcatcg acctgcagga
    50521 gcagatccgt caggagatcg gccgaccgaa gcgggtcttc gtgccgccgg ctatcgatcc
    50581 ggaccttgcc gcgcgcgtgg aagcggcggc ccggcccggc tttacggacg gctatgcgac
    50641 ggccgggaag ctcgaccgct acgcgaaact ggatgagatc cgggcggcgg tggtgaagga
    50701 attcacggcc gaggccccgg acctgaaatc gcaggtcctg accatcctgg agcatctgga
    50761 gcggaagatc ctgcggggga tgattctgca cgagaagaag cgcagtgacg gacgctccgc
    50821 gacggagatc cgcccgatcc gttgcgacgt ggggatcctg ccccgggccc acggctccgg
    50881 cctctttacg cgtggggaga cgcaggcctt ggccgcgctg acgctgggga cgtcctccga
    50941 cgaacagcgc ctggacttca tcggtggcga ggagatgcgc tcattcatcc tccactacaa
    51001 cttcccgccc tacagcgtgg cggaggcgaa gagcatccgc ggtcccggac gtcgggagat
    51061 cggtcacggc aacctggcgc gtcgggctct cctgcccgtc ctgcctgcgc cggaggtctt
    51121 tccctacacc atccggatcg tctccgagat cctcgcctcc aacgggtcgt cgtccatggc
    51181 gacggtctgt gctggggtcc tgtcgctgat ggacggcggc gttccggtca aggacgtcgt
    51241 cgccggtatc gcgatgggcc tcctgaagga gggggacgag gtggtcattc tgtccgatat
    51301 tctgggggac gaagaccacg ccggcgatat ggacttcaag gtctgcggaa cggagaaggg
    51361 cgtcaccgcg atgcagatgg acatcaagat cgacggtctg acgggcgaca tcctgagggg
    51421 cgccctgcgc caggcgcggg acggccgctg tttcatcatc gaccggatcc gggagacgat
    51481 ggcggcgccg cgcgacgaca tctccgttca cgccccccgc atcaccaccg ttaaggtcaa
    51541 gcctgagaag gtgcgcgacg tgatcggatc gggcggcaag aacatccgcc agatcatcag
    51601 cgagaccggc gtgacgatcg atgtcgagga cgacggcacc gtcacgatcg cgtccagcga
    51661 cgcggacgcc gccgcccggg ccatcgccat ggtcaagtgg ctgacggagg aagcggagat
    51721 cggcaagatc tataccggca cggtgaagaa ggtcgtcgat ttcggggcct tcgtggaaat
    51781 cctgccgggc acggaggggc tcgtccacat ctcccatctg gcgaaggagc gcgtcaacaa
    51841 ggtgaccgac atcgtccacg agggcgatga ggtgatggtc aaggtcctgg agatcgacaa
    51901 gcagggcaag atccgtctga gcagaaagga agccctggat gcccatggtc cggaaaaccg
    51961 ttctcgataa cggcctgacc gtcgttaccg aagaaatcga taccgtccgg tccatctcca
    52021 tcggcgtttg ggtgcgttgc ggttcccggt acgagtcggc ggccgacaac ggtatggcgc
    52081 acttcatcga gcatatgctc ttcaagggca cggagcgccg cagcgccttc gacatcgccg
    52141 cggccatcga ttccgtgggc ggggtcatga acgccttcac gggcaaggaa ctgacctcgc
    52201 tctacatcaa ggtgcccgat taccatctgc ccctggcgat ggatctgctg gcggacatcc
    52261 tgcggcagtc ccggtttgcc gccgaggaga tcgaaaagga aaaggccgtc gtccggcagg
    52321 agatccacat gctggaggac tcccccgacg agtatgtcca cgagatcttc gagaaggcct
    52381 tctggggcgg gcacgccctg ggccggtcca tcctggggac gaaggatcgg gtcgccggct
    52441 ttacgcggga tgggctgaat gcgtttttcg accggatgta ccggggacgg aacatggtgc
    52501 tgagcgccgc cggtcacctg gaccacgcgg tgttccttaa gcaggtcgag accgcgttcg
    52561 gcgccctgcc cgaggggcgg cggcaggcgt ttccggatgc accgacgacc gaggcccggc
    52621 aggtggtgca ggagaaggat ctcgaacagg tccacctggt cgtcgggacg gtggcccccg
    52681 ccgcccgcag tccggagcgc tatgccggat ttctgctcaa cgcggttttc gggggcagca
    52741 tgagttcccg cctgtttcag gaggtgcggg agaagcgggg actcgcctac gccatccaat
    52801 cgtacctcgt gccttaccag gatacgggca tcctcggggt gtacgccggc gccgacgccg
    52861 accgggtgga agaggtggcg acgctgatcc aggcggagat gagccgtctc cgacggacgc
    52921 cgctgacggc ggacgaactt caggcggcaa aggagttgat caagggaaat tttctcctga
    52981 gcatggagag cacggatcac cgcatgactc ggctggcgaa gaacgagttg tgtctcggcc
    53041 gtcagacgac cgcggaggac gtgctggccg ccgtggacgc cgtcacctgc gaggacatcg
    53101 cccacctggc cggcgagctg ttccgttcgg aaaccctgtc cgcggccgtc atgggtcctg
    53161 tccgtccaaa gcaggccttc gattttcgca tgacgtagga ggggtggcgt gaacgtcgac
    53221 gcccttgggg aggaagacat cgaaattccc gtgcagcgtc tcgcggggac ggaagacctg
    53281 cccctgcccc gttacatgac ggaacgggcg gccgggatgg atgtccacgc cgccgtcgtg
    53341 gaggatacgg tggtgccgcc gggcggacgc cggctgatcc cgacgggttt gaaagtcgcg
    53401 gtgccgccgg gccatgaggc cgagatccgt ccccggagcg ggctcgccgt ccggcacggc
    53461 gtgacggtcc tgaacagccc cggcacgatc gacgcggact accgggggga gatcggcatt
    53521 cttctgatca accacgggac ggagccgttc gtcgtgaagc ggggcgaacg gatcgcccag
    53581 atggtggtcc gccgggtggg gcgggtgcaa tggcgggagg cctccgcgct ggaagaaacc
    53641 ggacgcggcg agggcggatt cggccatacc cgccgctgat ccgggcggac gggcaggaaa
    53701 gcgtaatgaa gaagttcaaa gtccagaaat cgttccagga gatcaacgag cgaatccgcc
    53761 gagggaaggc cgtggtcgtc acggcggagg agatgatcga catcgtcgag cggcacggtg
    53821 ccgtggacgc ggcccggcgg atcgatgtgg tgacgacggg gaccttcagc cccatgtgtt
    53881 cttccggggc ctttctcaat ttcggccata cgtcgccgaa gatccgggcc tcccggatct
    53941 ggctgaacga cgttcccgcc tatggtgggc tcgccgcggt ggactgttat ttgggggcca
    54001 cggaaatgac ggaggccgat cccctgaaca gcgtctatcc cggtgagttc aactacggcg
    54061 gcgggcatgt gatcggggac cttgtggcgg gcaacctcat ccggctgcgc gcggagggct
    54121 atggtacgga ttgttacccg gcccggcgct tcgagaagac catcacgatt catgatctgc
    54181 gcgacgccac cctgttcaac ccgaggaacg cctaccagaa ctacagctgc gccgtcaaca
    54241 tgtccgacaa gaccatttac acctacatgg gcatgctgcg cccgcggatg ggaaacgcca
    54301 cctacgccac gtcgggccag ttgagccccc tgttcaacga cccctactat cagactatcg
    54361 gcatcgggac acgaatcttt ctcggcgggg cgcaggggtt tgtcgcctgg cccggcaccc
    54421 agcacaaccc ccgggcgccg cgcgggaaga acggcgttcc ccgcgagggc gcggggacca
    54481 tcgccaccat cggcaatctg aaggagatgg cgccggagtg gctgtccggc gccagcatgc
    54541 tgggatacgg ggcctccctg tacgtcggga tcggcattcc catcccgatt ctgaacgagg
    54601 ggctggcccg cttcaccgcc gtcaaggacg aggacctcta tgcccaggtc tacgattacg
    54661 gcatggacta cccgaaaggc gcggcgaaga gcctgggaga ggtgagctac agggatctgc
    54721 gcggcggaga gatcgtcctg aacgggaaga ccattcccac cgcgcccctc tccagttatt
    54781 acaaggcaag acaaatcgcc gccctcctca aggagtggat cgagcgggga acgttcctgc
    54841 tcggggaacc ccagcaactc ctgccgtcga tcggcgagaa gtccgacccg tcctgagcgg
    54901 gaaagaattt ttgttgcgcc tcaccgcacg atagggtatg ggaaaagaca tcgttgcggg
    54961 aacttcggga tctcggaact cggggggcat ccatgaaggt tgggcgttat ctgctagtgt
    55021 tcgtcgtctt catggcgttg attatttcct tcggcaatcg gggactggtc gacaactaca
    55081 agatgcggga aaagctgcaa gacctccaga gagcgaatca agacatcagc gatgccaacg
    55141 gggagctgaa gcggtccatc atgctgttgc gcagcgatcc ggccaccatc gagcggatcg
    55201 ccagggacga actggggatg gtcaagccgg gagatttcgt ctatcgcttt ggaaaatgag
    55261 atccccgaga atttttttct ttacttatgg atatttgatt gttaaaattc ttccccatcc
    55321 gttagaacga acgaagcgtg agagcttttt gtggaaccgg gaatgatcgg gacgttgcgc
    55381 gtcattagcg gagtccgaca cggcagggtg gatccatggt ggatatcgta gggttgttta
    55441 ccggcgccaa gaagctggtc gggctcgata tcggatcgag cgccgtcaag cttgccgaaa
    55501 tcgtccagac gccgaaaggg ttcgttctca acaactttca tcaaacgccc ctcccgcggg
    55561 gggtgatcac ggacggcgcc gtcaccgagc cggacatcct gacccagcac atcaaagacc
    55621 tctataagaa agccggctgc aagcgcaagg ccatcgtgac gtcgctgtcc agtcacgccg
    55681 cgatcgtgaa gaaggtcaat ctcgcccaga tggaggagaa cgagcttcgc gaactgattc
    55741 acgacgaggc cgggaagtat ctgcctttcg acagcatgga cgacgtcctg ttcgactttc
    55801 agatcctggg cgaaaacgag tacaacccga atcagatgga cgtgctcatc gtggcggcca
    55861 agaaggacat cgtcgaggga ttcaccggcg ccattgagaa agccgggctc cccgtggtga
    55921 tcatggacgt ggattccttc gccctggagc acatgtacga acagaattac gacttcgagg
    55981 aaacggacgt cgccgccctg atcaacatcg gggcgagcat caccaacatc aatgtcgtcc
    56041 tgaacggcgg ttccatcttc acgcgggact tcagcctggg cggcaacgcc gtcaccgagg
    56101 ccctgcagga aaaactcggc gtcaccttcg aggaggcgga gaagatcaag atcgaaggcg
    56161 tacgcggcgg cgagatggaa gagcgggagt tcaacgacag cctgctgcag cttgccgacc
    56221 cgatctgttc ggagatcgag cggtccctcg attatttccg ctccacttac ggtggggggg
    56281 acatccgcaa ggttctgctg tgcgggggaa gcgccaacat tcctggcatc gcgcaggaac
    56341 tttcccagag gcttagcatc gaaacggagg tcgtcgatcc gtttcagaag atcgcttaca
    56401 ataaaaaggt gatcgacccg gggcagatcg aaagcatcgg tcccgtggcc gcggtcggag
    56461 tgggactggc gctccggagg ttgggcgaga aatgatcaag atcaatcttc taccctaccg
    56521 ggaaaaggcc gcgaaggccg gcatggcacg gcagatcatt ctgaccgccg ggttcctgat
    56581 cgtcctggtt ctgatcctgg ccggcaccca gatctacctg tccgcgaaca atgcgcggat
    56641 ggaaggcgag atcaagaccg ccgaagaaaa gctcgtcacc ctgaaccaga aggtcggcga
    56701 cgtggagaag ttcaaggaga acaagcgggt cctggaacgg aagctctccg tcatcaacgg
    56761 cttggaggag aaccgcctcg ctccggtcaa gatgctggcc gagctctccg gcctcgtgcc
    56821 cacccgcgat gtctggctcg acaagttcac ccagcggggc agggagctcc agatcgaggg
    56881 tgtcgcccgc gacaatacgg cggtctcccg ctttatgtca agtctcgagc gggccagttt
    56941 tatcagcgcg gtggagctcg tgtcctcgaa agagaaagag gtctcaaacg tcaagctgca
    57001 gcaattcacc ctgacctgtc agatgaaaaa gggcgcctga ccatgccgat caccgttgac
    57061 gacatcaaga agctcccccc gaaaatacag gccctgattc tcgtcggcgt ggtgcttctg
    57121 ctgggctact tctattactt cttctttctc caggcgacgc tggaaaagcg ggggcagctg
    57181 cagaccaaac tcgccgaact ggagcagcag gtggcggaga aggagcgtgt cgccgcccaa
    57241 ctcaccaaat acatgcggga ggtcgagacc ctggagcagc agttcaagat cgcgctgtcc
    57301 aagcttcccg acaagaagga aattcccgcc ttgctcaatt ccgtcgccct ggccggcaag
    57361 ggcgcgggtg tccggtttct gctttttgcg ccgaagccac ccgccaggcc ggtcgagaag
    57421 cctggggtca aggacaatct gaagccctcc gacaagcggg ccgaagagaa gggtgcggcc
    57481 ggcagcgccg gggcgccgtc ggccaaggcc gggggggagg ccttctatga cgaaatcccc
    57541 gtcgatgttc agatcgttgg cggttatcac aatatcgcgc tcttcttcga gcgggtggcg
    57601 aggcttcccc ggatcgtcaa tatcgaggac gttgtcatgg gggatgccgc ggcggcggcc
    57661 gataccaagg gacgcggccg ccccctgaaa acgtcctgta tcatcaagac gtacatgttt
    57721 gtcgagaggc ccgatgctgc agtcaaaaag acggatcaaa aagccaagta atcccgtgac
    57781 ggcgatcggg gtcggcctga tctgctgcgg actctggctg ggagccgggg tggccgcggc
    57841 cgccgatccg ggtccgcttc gcccttccgg gcccgtgccc gccctggccg cgaaggtaaa
    57901 gaccgccaag acgccggagc aggtgaaggc ggagaagacg ccgcaggccc agtttcgcta
    57961 cagcccgacc ggcaaggcgg atccgttccg tcccttcctg gaacgggatg aaatcgcccg
    58021 caagaaggcg atcaagtcca aaccgaagcc cattcccctc tcgccgctgc agcgcgtcgg
    58081 catcgaaaag ttcaggctcg tcggaattgc gggcggcgcg gcgcggcgcg tcgcggtcgt
    58141 cgaagacgcg gccgggaagt tctacaccgt caagccggga accattatcg gacagaacgg
    58201 cggccgggtt gccgagatcc aggaagaccg ggtgatcgtc gagcaaacgg tgtcggatca
    58261 gaagaaacgg accaaggtca accgcgtggt catccagctt cgcaaagaag attatgaggg
    58321 aagattatga aacgaaaatg gccatggcta tggacctccc tgcttgcggc gtctctcatc
    58381 ctggccgggg cggtgcctgc cgcccttgcc gcagccgaga cgtccgtcgg ctacgtcgag
    58441 gagatcaccg ccggtcccct gccgggcaag gagcgcgtca ccctggtcct gtcccggatg
    58501 acggccccga ccctggaggc cgtgccgggc ggcggcgtgc tgatcaaggt gccggacatg
    58561 ttcatccccg agggattgcg cagtccgttg ggcgaaggcg ccctgagcaa catccgccgc
    58621 atcgttccag aacagcggac ggtggagggg cgccagtggg cgtatgtccg cgtcgatctg
    58681 aagcagccgg tccggtactc cgtccggcag gaagggccgt cgctcatcgt cgatttcaac
    58741 gtggcgggtc tgaatgagcc gccggcggcg acggagcgaa cggctgccgc cgcggaagaa
    58801 gcgcggccgg cagcggcccc gaccgccgcg gctcaagaac cgctcccggg cggggatgaa
    58861 aagcggtaca gcggcaagcg gatctcgctc gacttccagg atgcgaacat cagaagcgtt
    58921 ttcaggctgc tgtcggaagt gagcggcatg agcctcgtgt ccggcgacga cgtgaagggg
    58981 aacgtcaccg tccacatgaa caacgtcccc tgggaccagg ccctcgacgt catcctcgat
    59041 atcaaggggc tgacgaaaaa gcagatgggc gacgtgatca gcattatcac gctcgcaaag
    59101 gccaagaagg acgaggccga ccggcagtcc aaggaagagg cccgcatcaa ggcggaggcc
    59161 ctcgagaagg agcgtgaaca gaagctcaag caggtcaagg gcaagctccg ccagatctcc
    59221 atcgaggcga agatcgtgga ggcggaggag ggcttcggcc gggacctcgg gattcagtgg
    59281 ggcgttggga aaaagagcag cttcggttcg cactacgatt acggcgtcgt tgcccaggcg
    59341 gcgaacagcg ccatgaccgg tgacgtcgcg agcggcggga atgtcaaggg attggcggcc
    59401 ggaagggccc tcacggcggc cggaacggcc ctcacggcgg ccggcctggc cgtcaacttc
    59461 cccgtggccg cggcggcccc cgccctgggg ttcgtccttg agggcgccca ctggatcctc
    59521 gacgccgaat tgctggcgca ggaactgacg gaccgggtga aggtcatctc ctcgcccaag
    59581 gtcctcacga tggaaggcgt cgaggccagg atcaagcagg gcgagcagat tccgaaggtg
    59641 accccggcaa cggcggagaa cccgcccacc gtcgattaca aggacgcctt cctcgaactg
    59701 gccgtgacgc ccaagatcac cgacgacggc cgggtctcca tgaagatccg ggcgtccaac
    59761 aactcggcca actatgcaaa ggcgattatg caaaaccctc ccatcaacac gagcgaggtg
    59821 gaatcgaatg tcgtgatcaa cgacggcgac acggtcgtga tcggcggcgt cctcaaaacg
    59881 accgagagca aggggggcga gggggtgccc tggatttcca agattcccct gctggggtgg
    59941 ctcttcaaat acgagagcgt ccagaccggc aagcgcgaac tcctgatctt cgtgacgccg
    60001 aagatcgtca aggacgacgc cgtggttgcg gctgcgtcgc cggcctgatc ccgccggggt
    60061 ggttgacggg acgcgtctcc ggacgatccg gaatttgaag catgccaaga gatcgccttc
    60121 gataaggcga tctctttttt ttggggagag acggcttgac ttccgccttg acaggtccgg
    60181 gcgcctttgt tagcgtcggc caggatccgt gccgaaaaca ggcgagggct ttgttggtgc
    60241 ggcgctttaa gcggtcaggc ccgagaaagc aggggttcgg aaagacagga aggttcgatc
    60301 catggatgat gcagatcagc tccaggccgg cgagccggag cgcaacaaga gggatctttt
    60361 cctcgaccag gcgcgcgtcc gtctggaaca gaaccagccg cggctcgccc tggagatggc
    60421 cgaggcgcgg ttgcggcgtc ttccggacga catcgacgcc cggaccattc tctgccatgc
    60481 cctgatccgg atgggccgtc ccgacgaggc gcgggagatg ctgcggcggg tcgaggggac
    60541 cctgttggac ttcgcgagcc tctatgcggt gctgggggat tgccgcctca aggaagggtt
    60601 tccggaggag gcggcggcgt actaccggaa gtttctcgcc ttcaatccgc cggacgcggc
    60661 ggcggtccgg gaggtgtccg ggaagctgga cgcgctgacg ggagacggcg atgccggacg
    60721 gccggaagac gagcgccccc ttcgggtcgg ggacatcgct cccgatttct acaccgtcac
    60781 cctggccgat ctgtacctgc gccagggtca tccggagatg gccgtcgacg tcctcacccg
    60841 gatcctggaa aaggacccgg aagacgtcaa ggcccggtcg cgcctccggg agattcaggc
    60901 cgtctcggag ccccgcccgg tccggcgtcc gggagggcac gcgcgcacga tcgggacgct
    60961 ttcccggtgg ctcgtgaagc tcgaagcatc gaggccgcat gcctcctgaa gcgaccccct
    61021 acgcccggag acggtcgcgc ctgtccgatc gcctgcccga ctggggcctc gacgccctgc
    61081 ttctcaccga agaagccaac ctccgctacc tgaccggctt tgcggggacg gacgcggccc
    61141 ttctgatgac ggaaagccgt gcgtcgctgc tcgtggacgg ccgcttcctg acggaggccc
    61201 gcgagacggt tccggaggtg gagatcgtcg aaatccggga cctgatcgag gggatcgcga
    61261 cggcactggc cgatgccgcg ccctcccgcg tcgggttcga aaccgccgtt ctgaccgtcc
    61321 acgtccacca gcgcctgctc gccgcgctcg gagcgggggt gtcgctgcgg ccgctcttgg
    61381 acgaactgtc gacgctgcgg gccgtcaagg acgaagagga agtccgctgc atgcgaaagg
    61441 cggcggcgat cgcctcctcg gccctgcagg cgatcctgcc ggatctccgg cccggggtcg
    61501 ccgagcggga catcgccctg gaactcgatt tccgcatgcg gcgtcagggg gcggagggaa
    61561 gcgccttccc gacgatcgtg gcgtccgggc cgcggtccgc ccttccccac gccactcccg
    61621 gcgcccggaa gatccgggcc ggcgaggggg tcgtgatcga ctacggggcc gttttccaag
    61681 gctaccggtc cgacgagacc tgcacggtgt tcgtcggtgg cggtccggat cgcgaggcgc
    61741 tcgccgccta cgcgcagatc ctcgaggccc agacgctcgc catcgccggc cttcgggcgg
    61801 gtgtcacctg ccgcgacgtg gaccgcatcg cccgggacgc cctccgccgg ggcgggtggg
    61861 accgcttctt ctcccacggc accggccacg gcgtcggtct cgacgtccac gaggccccgc
    61921 ggctcaacgc ccgctcggag agcacccttg aagcgggcat ggtcgtcacg gtggaacccg
    61981 gggtttacct ccccggtcgc tgggggatcc gcatcgaaga catggtgctc atccgcgcgg
    62041 acggcttcga ggtcctgagc cgggtgccca agacgctgac cctcctgaaa tgacgagaaa
    62101 aaataagttt ctctattgac attcgcccgg aattctttta ctggcaggaa aacctgtcgt
    62161 tcaccgcagg tattgcacgt agaacgtccg cagaggatgg gggcaatgaa ggtatttcct
    62221 gaagactaca tgtacagtcg ggagcacttg tgggtcaggg tcgacggtga cctggcgacc
    62281 atcgggatca ccgattacgc ccaggaaaag ctgggcgaag tcctctcgat cgaactcccg
    62341 gagatcgaca ccgaggtcga gcgggacgaa tccttcgggt cgctcgagtc cgccaaggcg
    62401 gtgatcgaac ttatcgcccc cgtgggcggg caggtggtca atgtcaatga cgatcttttg
    62461 gacgacgtag gcatcatcaa cagcgacccc cacgataccg ggtggctgat cgtcatcgaa
    62521 atgagcgacc tcgatgaaat cgatgatctc ctcgacaccc gcggttacaa cgatttcatc
    62581 ctggaaagcg aggaggactg atccggtgtg tccaactgga gactgatccc ctttcatgta
    62641 tccgatgcct tcgagaacat ggccgccgac gaggccattt tccgcctgat gcccctgggg
    62701 catacgccgc cgaccctccg cttctacggg tggcgtccgg ccgccgtttc gctcggttat
    62761 tttcagaaca ctgcgcaagc ctgcaacctc gcggtctgcc gtcgggaggg gatcgacgtc
    62821 gtgcgtcgtc cgacgggggg gcgggccgtg ctccatgacg gagatctgac gtacgccctg
    62881 tgcgccggcg tggacgagcc gccctttcct cccgggatcg ccggcgccta ccgcgtgatc
    62941 ggccggtgtc ttcaggaggg cctgcaccgt ttggggatcg acgcgacgcc cgcgcccggg
    63001 aacccggcgc cgcccggggt gcgcgacggc tccgtcgcct gcttcgcacg gacggccagg
    63061 gatgaactgc tcgtcggggg ccggaagatc tgcgggtcgg cccaggccag gtcgcgcggc
    63121 gccttcctcc agcacgggtc gctcctggtc cggttcgatg cggcacgcgc gtcccggtgt
    63181 cttgtggccg aagcggaaga gcgggagcga acccgccgga tgctgacgcg ggccgtgacc
    63241 gccgtcgaca cctgcctggc gccgccgccg gcgatggacg caatcgtccg cgtcctggcg
    63301 gaggggtttg ccgcggtcct gcatgcggat ctgcgaccgg gcgccctgac cgacgcggaa
    63361 gaggccctga aggcgcgcct gaagcgggaa aaatatcggc ccgaggcctt aaacggcgaa
    63421 ggagaaccgg atgaaacggc tgcacctcat tagggaagag atccggcgac agcgggccta
    63481 tcccgcggac gccacgccct gccaaatcaa gctggatgcg aacgagaacc ccttccctct
    63541 gccggaggac ctgaccggcc tgttcttttc ggccgtgaca cgggaggtgg ccctgaaccg
    63601 ctatccggag gcgggttccc ccgccctgag gcgccggttt gccgcgcgct tcggggttgc
    63661 gcccaatcag gttctgctcg gcaacgggtc cgacgaattg atcctggccc tgctcatcgc
    63721 ctgcggcggc cccggcaaga gcgtgctgat cccgactccg accttcgcca tgtaccggat
    63781 ctgcgccttg aacctggggt gccgcgtccg cgaggcgccg ctcgcggcgg gcggagacct
    63841 ggacctgcca gcgatgctcg cggcgctcga acgggagcgg ccggtcctga ccttcctgag
    63901 ttatcccaac aatccgacgg gcgggtgttt cgacgacgag aagatcgccg ccgtcatcga
    63961 acgggcgtcg ggactggtcg tcgtcgacga ggcttatggc ccgttttccg gccgttcctt
    64021 tctcccccgc ctcgaggcgt acgacaacct cctgatcctg cggacgctct ccaaggtcgg
    64081 actggcggcg atgcggatcg gattcctcgt cggtcccccg gaaatcgtgg ccgagatcga
    64141 caaggtccgt cttccctaca acctgaacag cgtctcacag atggcggccg ggttctatct
    64201 cgatcacgag gcgcggttca tggcccagac ggaggagatc gtccgggaac gagtcggact
    64261 gtgggatgct ttacgacaaa ccgcggggat gcatccctgg ccgacggatg caaatttcat
    64321 tttttttact tgcgatcgcg atataaatca ggtatatgct gcacttgtca aaaggggtgt
    64381 cctcgtcaag aaattcgacg cctcgggaag cgcgccgccg gcgatacggg tcacggtcgg
    64441 ccgccgcgag gaaaatgagg cgtttctcaa agcactccgg gcaggggtta caacgttagg
    64501 ggcgtgattt aatctccagg caaaacaaag ggtagtcggc gacacggcgt caagagaagg
    64561 tgtcgagctg cccttttttt gcgccttttt aaagctctaa taaaatcaga gagttatgta
    64621 atgaagatga tcttgcttct ggaagacggc acgtctttcc ccggggagtc cttcggggcg
    64681 gtcggggaag cgacgggatg ggtccatgac gagcgcgggg tgatcggcta tcaggaatgc
    64741 ctgactgatc cggacaatcg gggctgcatc gtcaacatga cctatcccct gatcggcaac
    64801 tacggggtga acgacgagga cggggaatca gaccgggccc aggccgcggc cctcctggtc
    64861 aaggagaaga gccgggtcgt cagcaactgg cgcgccggat cgaccctcga ggccttcatg
    64921 gaccgctggg gcgtcgtggg cctggagggt gtcgatacgc gcgccctggc cctacacctg
    64981 agggaacacg gggagatgcg cgggatcgtc gccccggccg catctccgac ggcggccctc
    65041 ctggcgaagg tccgcgccga ctcaggcccg ctcccggcgg atcgccccag gggatcccgt
    65101 tcccatgcgg tttcggcccg cccctttgaa acggacggcg gtccgcgcat cgccatgctc
    65161 gatttcggct gccggcggtc ctatctgcgc cagctggagg ccctgtcgtg tcaggtgcgc
    65221 tccttttccc cgacggccgc ctgggaggag atccggagga gcggtcccga cgggctgttc
    65281 ctctcgaacg gtcccggcgc accggaggcg ttcgagccgg cccggcgcat gctggcggcc
    65341 gccctgggcg aacttcccgc gttcggaatc gccctggggt gtcagatgct cgccatcgcc
    65401 gcgggcggga aggcgacgcg catgggtcag gggcatcacg gcgccaatta tcccgtgcgg
    65461 gatctccgga cgggccggac ggcaatgacg ctccagaacc acggttgggt catcgacgag
    65521 acgtcgttga agggcggcat ttgcacggtg tcgcaccgga acctcaacga cggcacggtc
    65581 gagggcgttt cgtcggagcg ggggcggttt ctaggggtgc agttctctcc cgttccggat
    65641 gacgacggcc gtccgagcgc gttgttccgg gaattcgtgg agaggacgac caggacgggc
    65701 ggaaaaaggc agaaggcaaa aggttaaggg cggaggggaa gagaaaaagg gaaaacgatg
    65761 ccgaagcgaa ccgatctcaa aaagatcctc atcatcgggt cgggaccgat cattatcggc
    65821 caggcctgcg agtttgacta ttccggctcc caggcctgca aggccctcaa ggaggaaggc
    65881 tattccgtca tcctcgtgaa cagcaatccg gcgacgatca tgaccgatcc cgagatggcg
    65941 gaccggacct acatcgagcc catcaccccg gagattgtgg agaagatcat cgcccgggag
    66001 cggccggacg ccatcctccc cacgctgggc ggccagacgg ggctcaacac ggcggtcttt
    66061 ctcgcccgtg ccggtgtctt cgagaagtac ggcgtagagg tcctgggggc gtcggcggag
    66121 gccatcgccc gcgccgagga ccgggatctc ttcaagcagg cgatgctcga gatcggcgtg
    66181 ggggttccgg acagcggcat cgccaccacc gtggaggagg gcatggccat cggcctcggc
    66241 atcggcttcc ccctcatcct gcggcccgcc ttcaccctgg gcggcaccgg cgggtcgatc
    66301 gcctacaaca aggaagaact ggaggagttc ctcgccaagg cgatcgaata cagccccgtg
    66361 gggcaggtcc tggtcgaacg gtcggtcctg ggctggaagg agatcgagtt cgaggtcatg
    66421 cgggacgccg ccgacaacgt catcatcgtg acctccatgg agaacgtcga cgccatgggc
    66481 gttcacacgg gcgacagcgc cgtcgtggcg ccggcccaga ccctgacggc ccccgaatac
    66541 acccatctga cggctctgtg ccgcaagatc atccgcaaga tcgacgtcac cggcggcgtg
    66601 aacattcagt tcgccgtgag ccccgacaac ggcgaggtgg tgatcatcga ggtcaacccg
    66661 cgcctgtccc gtagttcggc ccttgcgtcc aaggcgaccg gtttcccgat cgcccgcgtg
    66721 gcgacgaagc tggcggtggg gtttaccctg gatgaagtca aaaacgacgt gaccggccgg
    66781 acgtcggccc tccatgagcc gacgatcgac tactgcgtct tcaagatttc ccgtttcacc
    66841 ttcgagaagt ttccgctggc cgacccgacc ctgaacacct ccatgaaggc cgtcggcgag
    66901 gccatgtcca tcggccggac ctttaaggaa gcctttcaga aggggctccg ctccctggaa
    66961 acggggcgct tcggtttcgg cgccgacggc aaggatgtcc ccttcgaggg ggacggcacc
    67021 gtcgagaaga ttcgcgagaa gctgcggatt ccgaacgcgg agcgcttctt ctacatccgc
    67081 tacgccttcc aggccgggct cacgatcgac gagatctacg acctcacgcg catcgaccgc
    67141 tggtttctct tcaacatgaa ggagattttc gacatcgagg aagagctggt ccgctaccgg
    67201 ttccacggtg tgaagcggga cctgccggtc tggaagaagg acttccccgc ggcgctcctc
    67261 cggcgtgcca aggaatgcgg gttttccgac gtgcaactcg cccacatcct gggcgtcgag
    67321 gaggaaacgg tccggcggat gcgcaaaaag ctggggctca atcccgtcta caagctcgtc
    67381 gacacctgcg ccggagagtt caaggccgac cgtccctact actactcctc tttcgagacg
    67441 ctggaggaga gccatccctc ggaccgcgag aaggtcatca tcctgggtgg cggacccaac
    67501 cggatcggcc aggggatcga attcgactac tgctgcgtgc atgcctccct ggccctgcgg
    67561 gagctggggt acgagagcat catggtgaac tcgaacccgg agacggtcag caccgattac
    67621 gacacctccg acaaactcta tttcgagccg ctcacccgcg aggatgtcct caacatcatc
    67681 gagcgtgaga agcccgtcgg agtcatcgta cagttcggcg gacagactcc cctgaacctc
    67741 gccgtgccgc tcgagaaggc gggcgtgccc atctggggga cgacgccgga cagcatcgac
    67801 cgcgcggagg accggaagcg cttccagatg cttctgaaga agctaaacct gatccagccc
    67861 gacaacggaa ccgcccgttc gtacgaggag gcccagcggg tggcgaacaa gatcggctat
    67921 cccgtgatcg tccgcccgtc gtacgtgctg ggcggccggg cgatggagat cgtctatgac
    67981 gacgaggcct tgggagactt cgtgaagaag gccatccggg tctcgccgga gcatccgatc
    68041 ctgatcgaca agttcctgga ggacgcgatc gaaatcgacg tggatgccct ctccgacggg
    68101 gagacgacga tcatcggcgg catcatggag cacatcgagg aggccggggt ccattccggc
    68161 gacagcgcct ccgtgattcc cccccacacc ctcgaccagc gcctggttca ggtcgtcaag
    68221 gacaacacct acgccctggc ccgcgagctt catgtcagcg gcctcatgaa catccagtac
    68281 gccatccgca acgacgtggt ctatgttctg gaagtcaatc cgcgggcgtc gcggacggta
    68341 cccttcgtca gcaaggcgac gggggttccc ctggccaagc tggccacgaa ggtcatggcc
    68401 ggccggaccc ttgccgagat gggattgacg aaagaggtgg aaatccgtca cgtggcggtc
    68461 aaggaatcgg tgttcccctt cgcccgcttc cccggcgtgg acgcgatcct ggggccggag
    68521 atgcgatcca cgggcgaggt catggggatc gacgtgagct tcggcaaggc cttcgggaag
    68581 tcccagatcg ccgccgggac gaaccttcct cagaccggga aagtcttcat cagcgtgatg
    68641 aacaaggaca agcgccagat cgtctttctg gccaagcaga tggagcacat gggcttctcc
    68701 atcctggcga cggccgggac gggcggggtt ctgaagaaca acgacatcgc ggtgcagatt
    68761 ctccccaaac tctccgaagg gcgtccgaac gtcatcgatc tgatgaagaa ccgggaggtg
    68821 gcgctgatta tcaacacctc gggcggcaag aagccccgcg cggacacggt ctttatccgg
    68881 tccacggcgg tgctgcacaa cgttccgctg atcacgacga tgtccggcgc ccaggcggcg
    68941 gtgaacgcca tcgaggccat gcggaaagag gcggtgaagg tccggacgat ccaggaatat
    69001 caccaagaga tcggtatcgg gcgttagggg aaaggcgaac ggagggggag tgaataatga
    69061 agggcgttgg gcaatcggtg tcggacgtcg ggtgaagaaa atcttgaaaa aatcggtgtc
    69121 gggcgatacg catcgagaat gggtgaaagg gggacagaag cccccgacgc ccggtgccta
    69181 acgcctgacg cccaatgcac gatggaaagg aaggcagaat ttggaagtca aggtattcga
    69241 caatgatgtg gacaaggcgc tgaagatcct gaagaacaag ctgtccaaga gcggtctctt
    69301 caaggagttg aagctccgcc gggcttacga gaaaccgtcg gtcaagaaaa agcgcaaggc
    69361 catcgaggcc cggcgccggc ttgccaaggt ccagcgccgc cggtcctcgt aagacaaccg
    69421 cgggaggggt gacgtcgcaa gacgggacgt cggtttgacg ggtttcgccc gggcggcgac
    69481 ggtgtgcgtc cttcccgaag attccgcgcc ggcatgccgg gtttgcaacg gaccggctcc
    69541 gcgcctatcg gacggccgga ccgtccgggg aagcgaggtc gaggcgatgg aagaaaatca
    69601 atatctggtg gacgccctgt ccatcgagga atcctggcgg atattccgga tcatggccga
    69661 gttcgtcgag gccatcgacg cgctttccaa ggtccagcgc gccgtgtcta tcttcggctc
    69721 cgcccggacc tcgccggagg accccttcta ccagaaggcg gaatatctgg ccagacggct
    69781 ggtggaaaag ggcttcggcg tcatcaccgg cggcggcccc ggcatcatgg aagcagccaa
    69841 caaaggggca accgaggccg gaggaaagtc cgtggggatg aatatccggt tgcccttcga
    69901 gcagaagccc aacccctatg ccaacatctc catcgattac cggtatttct tcatccgcaa
    69961 ggtcatgttc gtcaagtatg ccgtggccta tatcattctc cccggcggtt tcggcacgtt
    70021 ggacgagctc ttcgaggccc tgaccctcat tcagacccgg cgcatcaaga gtttccccgt
    70081 catcctgatg gggacggcat actggcaggg cctcctcgac tggctcaagg ggagcctcct
    70141 cgcgaacggc atgattcttc cggaggatct cgacctcatc catgtgatcg acgatcccga
    70201 cgaggcggtc cggcacatcc tgaagtacgt gattgtctaa agccgtggtc cgccgcggca
    70261 ctcccactgg taacgcccat ggttgatcag gaaggtggat ccgcaccgaa tctgtccagg
    70321 gtcctgagcg acatcgcatc cggttgtccc gttccctgct atctgctgtt cggcgaggag
    70381 gaattcctcg tcaagacggc ccgggagaag ctgctgaacg ccctgctgcc ccccggggaa
    70441 cgggacctga acctcttcag cctggatggc gaaacggcgg aagcgggaca gatcgccgcg
    70501 acgctgatga ccccgcccct gcttcccggc cgcaaggtcg tcctggtcga gggaacgccc
    70561 ctgctgcaat cgaaaaaaac cctgccggac ctggagaaga gcgttcgtga acatctggat
    70621 cggaaccccg gacgggccat ccgcgatttc ctgctgctcc tccatctggc gggctggcat
    70681 ctcgaagact tggctgccga cggctggaaa aagatttccg cggaggagtg gaaccggacg
    70741 attggtggcg aaggcgcggc cgaacgcgag gcctggctgc cccgtatcct tgacgcttgc
    70801 ctggcgcggg gacgggacgc ggtccagggt gcagaggcga cggagcccct ggaggcggtt
    70861 cttcgggagg gccttcccga aggcaatcac ctgatcctga cggccgaggc cgtggacaaa
    70921 agaaagcggc tcttccaggc catcgccaag ttcggcgttg ccctggagtt ttctcccgtg
    70981 aagggggagg cgaaacaaaa gcagcgcctc caggacgtgg tgcgggaaaa aatcgcccaa
    71041 agcggcaaga agctgacgcc gaaggcctgg ttcgccctgg gtgaaaagac gggatttcag
    71101 ctgcgggcca cgacgagcgc cttagacacg ctcctgacct acgtggggga caaggtcacc
    71161 gtcgacgagg cggatgtcga ggccgccgtg gggaagaccc gggagggcac ggtctttgcg
    71221 ctgacggcgg ccctggaccg gaaggacggc cgggaggccc tgtttgtcct gaaggatctc
    71281 ttcgaccagg gcgtgaaccc cttggtcatc ctggccgtaa cggcccgcga gatccgcaac
    71341 ctgattcacg cccgtctgct gatcgactcc ggccgatttg ccgaaatcga acgggagccg
    71401 gactatggcc ggtttcagaa acaaatctat cccgagatca agtccgtgct gggcgtggcc
    71461 gggaagaagg agggggggct cgccggtcag catccgtatg tggtgtacca ggccctgaaa
    71521 aacgcgcggc ggtttacggc ggaaaccctg gtcgacgcgc tcgaggccct ggcggagatg
    71581 gatctggcct tcaaatccac gcctcgtgat cccgccctgg ccctggagcg gttcgtcctg
    71641 cgcttctgcg cctgacggga gacggttcag atctttctca gccgggcctg tacggccttc
    71701 tcccgccgcg atggccggcg ggccatgccc ttccgttttc cgccgcgtgg cgcatcttcg
    71761 gcatccggat cgtccggcgg cccggcggcg tctttcagga gggccttttc caggcggtcg
    71821 gcgaattcca gcgaagaaac ggcgacggcg ccccggcggc ccaggtgttc ggcgattccc
    71881 cggtccgagg tgacgaccag gacctcctcg cgcccctcgc gggcgagacg ccggatcacg
    71941 gcatccgccg tttcaccccg ccgggaatag cggatcacca ccccgcccga gcgatcgcgc
    72001 tcctcgaagg gcgatccccc ctgccagccg tcgaagacga cggtgatccg gtgtccccgc
    72061 tgccgctggt aggtgaccag ccgctggatc agggccagtc gccccgcctc caggctcgtc
    72121 cgctcatggc gtaggagttc cggcgactgc cggatgaggt tgtagccgtc gatcaggatg
    72181 tgcatggtcc ggtttctccg acaatggcga gggggcatcg ctctccttgg tgcccgtgtc
    72241 cgcagatgtc caggcgccat ggacacactg ttttcatagt cgatgggcgt cctttgtgca
    72301 acgtgaaaat cggcccccgc tctcgtgacc ggctttttgc ttgacttcag ccgggcaggt
    72361 ttgttaggga tcagcgtcag cgtatccggt cccgagacgt cctgccgtcc ggtgcgcttt
    72421 ccggactccc cggtatcata aggttcatcc catggacgta tcatcggaac aactcgataa
    72481 gacaagagac tcacgacaac atttgccaga ggaaggagtt tgaagatgga aatcaagaac
    72541 gtgtgcgttt tgggtgcggg cctgatggga aacggcattg cgcaggtctg tgcgcaggcg
    72601 gggctcaatg tgacgatgcg ggacatcgaa cagcggttca tcgacggcgg catgaacacg
    72661 atccggaaaa acctgagccg cgacgtggag aaggaaaaga aaacccaggc cgacatggac
    72721 gccatcctgg ggcggattaa acccaccctg gacatgaaag aggcggccgg ccaggccgat
    72781 gtggtcgtcg aagtggtcat cgaggtcatg aacgtgaaga agcaggtcta tcaggaactg
    72841 gagcagatcg ttcccgccca ctgcctgttc tttaccaaca cgtccggcct gagcatcacg
    72901 gaaatggccg ccatcacaaa gcgtccggat cgcttcatcg ggacccactt cttcaatccc
    72961 gttccggtca tgaagctcct ggagatcatc cgcggctacg agacctccga cgaaaccctg
    73021 gcggtcgcgt ccgagtgggg gaagaagatc ggcaaggacg tgatcgtggt caaggaggcg
    73081 ccggccttcg tcgtgaaccg gatcctgtgc tccatgatca acgaagcctt cttcgtcttg
    73141 ggcgaaggcc tggcctccgc ggaggacatc gacaagggca tggtcctggg ctgcaaccat
    73201 ccgatcggtc ccctggccct gtccgacctg gtcggcaacg agacgctgct cagggtcgtc
    73261 gagggattgc accgggaact gggcgacaag taccggcccg cgcccctgct gaggcagctc
    73321 gtccgtgccg gccgctttgg acgaaagacg ggcaaggggg tgtacgagta taagaaatag
    73381 gcgcaaggcc cgggcggttg agccgcggtc cacgccgtga aattatcttt tttaaaaagg
    73441 agatgggaca tggattttga attgacggaa gaactgcaga tgttgaagga catggcgtat
    73501 aaattcgcca aggccgagtt tcccaacatg tcgcatgaat gcgacgaggg ggagaagtac
    73561 acgccggaga tccgcaagaa ggccgcggaa aacggcctgg tcggggcctg gattcccgag
    73621 gcgtacggcg gcgccggcgc cgggatgttg ggcaacaccc tgattacgga ggagctctcc
    73681 aaggtagaca tggggatcgg cctgaacatc gtggcggcga ccttcggttc cgagtcgatc
    73741 ttccagtacg ggtcggaaga gcagaagcag ctttacctgc ccaagctctg caacggcgaa
    73801 tgggtcagcg ccggggcgtt tacggaaccc aacgcgggga ccgacgtggc gggctacaag
    73861 acccgggcgg tcaaggatgg cagcgactat gtcgtcaacg ggaacaagat gttcatcacc
    73921 aacggcaccg tctgcgacta catggtgacc cagtgcatca cgaaccccga ccagaagaag
    73981 cacaacagct tcagcctgat catcatcccc gccgatgcca agggcgtcac gcgcaacaag
    74041 atccacggca aactcggcat ccgttccagc gatacggccg agatcgccct ggaggacgtc
    74101 cgggtgccgc aggccaacct cgtggggaag gagggcaagg ggttctacca gctcatgcat
    74161 ttcttcgaca cgacgcgggt catggtctcg ggccaggccc tcgggatttc cgaggcgtgc
    74221 ctggaagcga gcgtcaagta tgtcaaggag cggacctgtt tcggggcgcc gctgggggcc
    74281 tatcagatga cccagatgaa gctcaccgag atggccatcc ggatcgaggc cctgcggggg
    74341 ctcgtgtaca aggccgcgtg gctgatcgac gccggcaagc ccgattacac cctggccgcc
    74401 atggcgaagt tcttcggcgg gcagacggcc gttttctgcg ccaactacgc ggtcgaactg
    74461 cacggggggt acggctatat cgacgagtac gatgtccaga agtggtaccg cgacgccaaa
    74521 atcctggagc tctacgaagg gacgaaggaa gcggagatca tgaccatcgg ccgggttctg
    74581 caggcctgag atcggcgcgg cgcatcgtcc tgcggggacg ccacactgaa tcgaagcggg
    74641 gcaggatccg gggattctgt cccgctttct tgaaggggag gccattcgtg aagacaagaa
    74701 ttacggaact tttcgggatc aagtacccca tcgtcctgtc cgggatgagc tggatcagcg
    74761 tgccctcgat ggtcgcggcg gtttccaacg cgggtggtct ggggatcctc gccaccggcc
    74821 ccatggatcc cgagcagacc cgccaggcga tccgcgagat ccgcaaaaaa acggacaagc
    74881 ccttcggggc gaacgccacg ctcctgatgc cgggagcggc ggccaacgcc aaggtgctcc
    74941 tcgaggagca ggtgccggtc atcaactttg ccctgggcaa gggcgactgg ctcgtccggg
    75001 agg
//
LOCUS       DAOOLJ010000007        73965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_195_length_73965_cov_17.224936, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000007 DAOOLJ010000000
VERSION     DAOOLJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 73965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73965
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195_length_73965_cov_17.224936"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLW16_02450"
     CDS             complement(<1..367)
                     /locus_tag="PLW16_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69798.1"
                     /translation="MDFILNEDQAMLRDTVRRIAEEKFAPIAAEIDEKETFPKESVKV
                     LAENGLLGVQIPEAYGGAGAGMLAMVLVVEEIARVCAATSVILTTQALASDPLLLAGT
                     EDQKKNFLYRLASGACLGAC"
     gene            complement(611..1087)
                     /locus_tag="PLW16_02455"
     CDS             complement(611..1087)
                     /locus_tag="PLW16_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69799.1"
                     /translation="MAHQVESERLVSVFAYPMPYEWSIGLYGSKFFQEIKQNRRFVGI
                     RCDGCGKVYVPPRRVCGPCFRELTELVTLSDTGIITAFSVVNYPFIDPNTGAQRPIPY
                     TYGYIKLDGADNIFSHIINEMDLSKIKVGMKVRAIFKDTAEMQGNIQDIKYFDIVR"
     gene            complement(1105..2265)
                     /locus_tag="PLW16_02460"
     CDS             complement(1105..2265)
                     /locus_tag="PLW16_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69800.1"
                     /translation="MGRRVAIVGVGQTYHKSHRPDVNSREMIREAVTAALADAELTIR
                     DIDAIVIGNMDHFESINYVDTYSVEGSGGVMKPIMKVSTGGTTGATVAIAAYYHVASG
                     LFDRVLAIGFEKNSESDTTGAIITCSDAIWDRFSYSGAIPSLATEATAYMEKYGATQE
                     DAAIIGERDRNNALNNPFAHLQRPLTVADIMASPMLAYPIKLGDICPRSDGACACVLA
                     ADGVAQKIARKPAWFKAAAVRHTYTWFGDSTDYNKGLVSMQRASKDAYQQAGITKPAE
                     EIDVAELYLPYTWAGLKWIEDIGFCGPGEGVRFVRDGNTHMGGKIPVNPSGGVISTNC
                     IGATAMLRVAEAAVQVMGKGGKRQVPDVKNALATGFGGCFWSDVVILSTERP"
     gene            complement(2274..3416)
                     /locus_tag="PLW16_02465"
     CDS             complement(2274..3416)
                     /locus_tag="PLW16_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69801.1"
                     /translation="MDNAAIVGVAQTKYERRKKDQILADMVFDVTRAALDDAGLAIGD
                     IDSVVTVSNDFFDGRTISGMAVGDAAGGEDKNISTVSGDGAFGAFYGLMRVLSGYKAT
                     IVVAHYKGSEGSVPITTNGMFDPVFHRHLGMDAISAAALQANTYMTRFGIREEDVAQV
                     SVKNHGNAKNNPYAQLPMDITVADVMKSRKIAEPLKLLDCCPISDGAAAIIIANEETA
                     ARTRKKPVWVKGVAHCCEEYLLGDRRLADPVALKDAARRAYAMAGITDPMAEIDVAEV
                     LEPFSYMEPLWLEGMGFCKPGEGASLIEKGITPMGGRLPVNPSGGALSANPILVAGLA
                     RLIEATLQVRGEAGARQVKNAETALAHGFNGPCGQSHCVWIVGGRK"
     gene            complement(3437..3892)
                     /locus_tag="PLW16_02470"
     CDS             complement(3437..3892)
                     /locus_tag="PLW16_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69802.1"
                     /translation="MAEWLKDVEPLTLKGQIAVPYSWWVGDTGSRFLMSLRDDRKILG
                     TKCPSCGTVFVPPRRSCGRCFVDIEEWVDVAAEGVIEAHTIVHYAYPVQPVKPPFAYA
                     LIKLDGADVGFLHLITKDLGKIQNGLRVKAKFKEERTGSILDIDSFELI"
     gene            complement(3980..5326)
                     /locus_tag="PLW16_02475"
     CDS             complement(3980..5326)
                     /locus_tag="PLW16_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB69803.1"
                     /translation="MDRYDAIILGAGIAGLGVGALLQGKGLKTLVLEKSKTPGGRCKT
                     FHLPGGWKVDSGTHAVDLGKYSACATLLKRLGKEIPWSRNIEGAMLYDEGQWKLLPEY
                     LNLTTSDDRELADLETWMRAVDETELDTLDRVSLNELIRWKIASPRIAEFLRTIAMCQ
                     TTLTDADHISAGEVVAIYGDALRLNVKEGESLAQVRMPLGGIETMMQAMEAAYTERGG
                     TLLTGTSVRRVNVQPGKPKGIVTDAETYRAPIAVIAMPIWNMLKILFLDEMARYAPAW
                     AERMPTLERETSASMGFTIGTKAPLFTTPYYLSAWRLPNVGLPLQILGHTLFDDTISP
                     ANHMIAFIGACCTPAQASDKAFREKTLAAFWELVKRMFPNVERDLLWRQDGFYVGIDG
                     LGRSPGMTGRYRPPVHLPEVPGLYFAGDCYTGRGVGMNAAANSAMICAEEILKAHA"
     gene            complement(5457..6677)
                     /locus_tag="PLW16_02480"
     CDS             complement(5457..6677)
                     /locus_tag="PLW16_02480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69804.1"
                     /translation="MNANDVVVVSAVRTPFGRFDGVLKTVLSMDLGVLALKEVVRRID
                     LDPKQVDEVYYGTCIPAEYAIYTNVPARQITLLAGFPDESISLTIDRACCSSMTALRL
                     GCRAIKSGDAEIVVACGAENMGNVPLIASAEKTRWGSRLGHVALEDVLFELGYSRKGF
                     APVATDAGHVALEYGVTREKQDEWALASHTKYFAAAKAGKYEVGGELMRIEIPQKKAP
                     IVLEKDEAPRENLSLEKMAALKTVYGSPTVTAGNAPGMNSGASAMVIMSRKKADALGL
                     KPLATIEACACAAGSPKYMACVPAQAIEKMFAKTGHTIDDVDVIEINEAFAAVTLVSL
                     KMLAKDDPAKWQALQDKTNVNGGAVAIGHPVGASGARVTMTMMYELMRRGGGTGVAAI
                     CGGLSQGEAILIKV"
     gene            complement(6809..8332)
                     /locus_tag="PLW16_02485"
     CDS             complement(6809..8332)
                     /locus_tag="PLW16_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB69805.1"
                     /translation="MKEKQYDAIVVGAGVGGLTVGTLMAKEGLRVLILEQCDRPGGRA
                     LSIKGKEICDNGFDWYKKVLAAQYSYLASSQPDIETIIKNRMLDGYTLDIGYHALSAN
                     GAGYMLDFEDQIGGLPEIKKHGGMWGNYYKGKLYHDVAGSKIDPELKRIAKENKIPFF
                     SFYADAHMMTDEQLDALEKVSFKQWADEKGISKSDVIFNHLHSVATLFSTINSPEDIS
                     IGEIFRYFKTAFGPKLMRGVLGYVGGFVENGVMEWSKAVVEKYRGLGGDILYNAKVKK
                     VLIENGAVKGVLVDTAEGEQTFTTGKVVSNIPAQQTFKVIDKAHFPADYPEKIERMYG
                     YGSYCPYIGLNKLVMPEDEAKMGLKNTCVLPKSEGFDFDVYICWNIQSALDPTSAPTG
                     KYLYTGYLPLTEKEATTPELVKKIVNRLPEFMEEVYPGFKASMDWKLDVVCWKLEGVA
                     KSISQAGTQKVPVKSEHVRGLYFAGDTARGYGVAMDCAIVSGMICAGEILGKDFGFH"
     gene            complement(8372..9391)
                     /locus_tag="PLW16_02490"
     CDS             complement(8372..9391)
                     /locus_tag="PLW16_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69806.1"
                     /translation="MNEMEKKNYLSMWNGRRGAERNPSNYQRRDDGNHIYKSTDYYEW
                     WYVDASFDNGYHLVVTFHYMNAFLRPIVPSIQLMVYKPDGTQTARYDLCDEKDIYACP
                     DYCDVRMGEAWLKDAGATYEMYIKIKGVGARLSLKPVVPGWKPGTGLLYHDEAQGAIG
                     GWVVAVPYAEVTGELYLKEETIPVRGACYHDHNWGNKAQFDIFAGWYWGRIHNDKYII
                     DYGWVIPRAQEAPVVSPLLIARPGEIVLSTDRMETKLEEMVRDEKTGRDYARKLVITT
                     DTEGVKMTLTLDTTRMIETMQLPKIADWNQYYYRFLANYTMDVEVDGRRDVVSGQMLH
                     ELMYL"
     gene            complement(9401..9856)
                     /locus_tag="PLW16_02495"
     CDS             complement(9401..9856)
                     /locus_tag="PLW16_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69807.1"
                     /translation="MEEIKHYEPFEVDQHLTANSRYYAGRMGSRFYVALRDQQKILGA
                     KCPKCNKVLWPPRSTCQVCFSELTETVEIGPLGTVETFTVVTYNEPVQPRKAPFVYAV
                     IKLDGADTGMAHFLDEVDLDEVHIGMRVRPVFARDRKANILDIQYFKPL"
     gene            complement(9901..10395)
                     /locus_tag="PLW16_02500"
     CDS             complement(9901..10395)
                     /locus_tag="PLW16_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB69808.1"
                     /translation="MVQKEQEWISIPMTVDVPYRFAAGQYLTKYLVEMRDNGKIFAVK
                     CPRCKRVQLPPRIVCAECHVKNEEWVELAQEGTLVAFTIMYLPLTDPTTGKPHEPPFV
                     YGSVRLDGATSVLDHFVNVEPDMEKVWVGMRMRLVLRPKEQRTGDLSDILYYEPLPGQ
                     VRPK"
     gene            complement(10408..11577)
                     /locus_tag="PLW16_02505"
     CDS             complement(10408..11577)
                     /locus_tag="PLW16_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69809.1"
                     /translation="MKRNVAIVGGGQTYHAARRHDVDQVEMVNEAVRRALDDAGMTID
                     EIDTVFLGNMEFFEGVHMTDCWLVHGTGAYGKPGLKITTGGTVGATTVCVGAHHIASG
                     LFDKALCIGFEKQEEGDTGAILSGVAHPLWGRSLAGAAVGYFATMGSSYMATWGATEE
                     HSAMVAVKARQNARKNPYAHLKLDITIEDALNSRMLSYPIRMLDMCPASNGACAMVLA
                     DEKTAKKRCKKPVWIRAMDTAHNEQFELDWRFVDTKRPIFSKVCAARMYKKLGVKKPA
                     EYFDVFEVYEPATWAELIWYEDLGLCGPGEGFRLVEKGMTNIDGPIPVNPSGGVLSTN
                     CIGASAMLRVWEAALQIRGDAGGHQVPKDVRRALTTGYGGTNWTVMTALSKTLDD"
     gene            complement(11589..12740)
                     /locus_tag="PLW16_02510"
     CDS             complement(11589..12740)
                     /locus_tag="PLW16_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69810.1"
                     /translation="MADRVAIVGIAQTKFAPKRADVSYGEMAYEVIEAVLRQTGLKMD
                     KDIDNSISCSHDIWDGQTISDIGITDVIGGHLRNEEKMALDGSTAVYYGTIGILSGEF
                     ECTLLMAHTKMSQTNRNIVNNDAFDPIYTRLLGFDYTSAAALQARRYMYKYGVTPEQI
                     ARVVVKNLANAKKNPVAHQSGDFTVADVLSSPLVASPIRKLDIAPDTDGAVAMILASE
                     KRARQIKKNPVWIKGIGTCYDAYYLGDRDLAGVFALEKAAAQAYRMAGIKNPRRQIDV
                     AEVGDDFSYQELLWMEGLGLCDRGAAGRMVESGATEIKGKLPVNPSGGLLGGVPINVY
                     GLNRVAEAALQVMGQAGDHQVSKDVKVAVAQGHSGFCGQHQCVVVLGKE"
     gene            complement(12864..14051)
                     /locus_tag="PLW16_02515"
     CDS             complement(12864..14051)
                     /locus_tag="PLW16_02515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB69811.1"
                     /translation="MMKEVWIVDGVRTPIGRMGGALSGFRPEELAAFALKGLLEKTKI
                     DPAIVEDVLIGHACTNNAAVNIARWAVLKAGFPVSVTAQTVERQCGSALQTINSAAMA
                     ILAGFGDVYIAGGCESWSNAPYLMERQKVPFSLMPNAFIGKEVGPTPETNAPMGIVAE
                     ILAEEWGISREEQDAFGLRSQTLAIKAIEAGYFKDEIVPITIPQRKGDPIVFDRDEHP
                     RATNLEKLAALRPAFKKGGTVTAGSSSGMNDGGVAVLVMSKDKAESMGLKPLGRFVTC
                     ALAGVEPKYMGIAPAYAVPKVLERAGLKLADMDVVECNEAFASQTLAVMKELKKNGHV
                     VDPEKWNPYGGAIAFGHPNGMSGGRLALAVLKHLQRTGGRYGLATLCIGGGQGIATIF
                     ERI"
     gene            complement(14171..14956)
                     /locus_tag="PLW16_02520"
     CDS             complement(14171..14956)
                     /locus_tag="PLW16_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB69812.1"
                     /translation="MDYKDIKLTKDGAVATITIDRPEVLNAIRDNTMYEIQDALNGIA
                     QDDAIRVVVLTGAGDKAFVAGGDISIMAKGAGYLDVIHGLPAGQQITWNIEHFQKPVV
                     ARLNGLALGGGTELALACDIRVAADTIMMGVPEINLGIIPGYGGTQRLPRLVGVGMAK
                     KMVLTGEHISAQEAYRIGLVDVLVPKAELDGAVAKLCKRLASKSPIALAMGKEAINMG
                     VQADLRTGLSIEARCFCMCFGSQDRVEGMNAFLEKRKPEFKGK"
     gene            complement(14999..15889)
                     /locus_tag="PLW16_02525"
     CDS             complement(14999..15889)
                     /locus_tag="PLW16_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69813.1"
                     /translation="MDVKNMTVLGAGTMGHGIAQAAISAGINVVLYDVAQEFADKGAA
                     QIRKSLGKRVEKGKLAQEQADATLKLLRTSTDLKDAAKDADFVIEAVPESLDLKKKIF
                     AELDKICKPAAILASNTSVLPVTAIAGATERKDKVVGTHFFNPAAVMKLVEIVMPIGV
                     AAETVETAMAVCKKMGKVPVRTKDTPGFIVNRLLGPLYMDAAQMVLEGTATAEDIDTA
                     MKLGAGHPMGPCELADFGGFDVIQMAQDAVFEYTHSESAKLNILYRKMLEAGRLGRKN
                     GKGFYDYHPDGTKTPFKIFG"
     gene            complement(15939..17507)
                     /locus_tag="PLW16_02530"
     CDS             complement(15939..17507)
                     /locus_tag="PLW16_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69814.1"
                     /translation="MARVMDDFFRQHAAEQPDQEALVYKDRRFTYRQYDEITDRVAMG
                     LLGIGVKRGDRVGIYVPNWPEFIFAYLGAAKIGAVAVPVSWRFTAQEIKFVLDDAGIS
                     ALVMAAGFMGMDFVQNLKAARKELPLLKKVVILEEDKAEAGMIPWARFLAEPGPALEQ
                     AQAAVEPDDPMLFLYTSGTTGIPKAAMLTHKNLISYTNGQNAASDYGKGHTLLLNIPL
                     NHVGGAVMAVIATLNSGNKLVIMDMFVPEETLQIIEKERVTIIGQVPAQYALELLNPN
                     VEKYDLSTIQTAIVSSQPCPSELILAIKQRMGVMPQNAYGLTEVSGAITFTHPSHGEE
                     KLKHSVGAPINTVEIAIMDDKFNILPTGEVGEITIKGDPVMKGYWKRPDEDARVFDPK
                     GYFHTGDMGRLDEDGYLYIVGRKKEMFIRGGENVYPPEVEEALAKHPDVLMSAVVGRP
                     DPVMGEVGRAYIMKKPGTNPTAEGVKAFLKDHLANFKIPEDIVFRDQLPLTLLGKVKK
                     LDLYQEMEKEFPKK"
     gene            complement(17815..19281)
                     /locus_tag="PLW16_02535"
     CDS             complement(17815..19281)
                     /locus_tag="PLW16_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69815.1"
                     /translation="MSHKPFKIPKNPEEVMTWINSIPMYREDQPLDLSFKSQEEIRGV
                     QDAALVKQMERLGKFSPYYREKFKEWGLDYPSIKTLDDLEKVPLTSKADFMKDRGESF
                     KLDMDFEHIMEYILYEITYTTGTTTGMPSRFYNTTYDMFMQSWMFRIGCKLCYLEPDD
                     ILMNLFPYHFIPHIGYYKTVHFASAVGMSMCWGFTGAPLPGFPYNIHRSMQQAIEDIE
                     KKKVNALSGIASYIRRLIMVAEEQGRDLSRINKIQALGEAVPKGMRDDIRRRLQGMGA
                     GDVYISNAFGFTEAQTAFQECQELGGAHSGTPQLAYFEAVDENGKRKPDGEPGLLCIT
                     HLDRRGTCLLRYLVGDIVAVTNKPCPHCGRNDQRIVTAVGSTYGTRTKELMKVKGTLI
                     NPETMRDAVANTPGVVEYQFVVTKEKADDPYSMDMLKLRVGADKGADLASLEKVLKEK
                     VKKAVELTPKVEFVPLSEIFNPGATLKATRIVDERPQE"
     gene            complement(19488..20207)
                     /locus_tag="PLW16_02540"
     CDS             complement(19488..20207)
                     /locus_tag="PLW16_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB69816.1"
                     /translation="MDIDLHIHTNRYSGCSNIDPAKLLKKARAVGLDGIALTEHGIRW
                     SDDQIEQLRRASGVRDLLIIPGQEVACYWRHGRFQGEFLVFGYPKSLGSSRSAEEVIE
                     LVHAEGGVVVAAHPFKKFRHGDEGYYGAGKQVYDFHVDGLEVEHPDYDDEARTQAAEA
                     MASMGIAGTGASDSHALHTVGICRTVFERPVKGVAELCEEIRSGRLKALNLVNGRGVQ
                     ARPSGPDAPSCETRAATPSLL"
     gene            complement(20215..21354)
                     /locus_tag="PLW16_02545"
     CDS             complement(20215..21354)
                     /locus_tag="PLW16_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69817.1"
                     /translation="MYVNYFGESHEMLRASIRKFVEREIKPHIEHWEEEGTFPLELYK
                     KAGDAGFLGLGSPEEYGGTPADIFHQVAYTEEILRCGSVGLVSSLGSSAIAVPPILAL
                     GTEAQKRKYVPPVLAGDQVAVLGVTEPSGGSDVANLLTRAVRKGDTFIVNGSKTFITS
                     GTRADYVTTAVRTGGSGHSGISLLVIERGTPGFTVSKKLEKMGWWASDTAELSFDDCV
                     VPAENLLGEENKGFYGIMLNFQKERLYLAVEAHAVAEMALEESIRYAKERTAFGKPLT
                     GFQVTRHKLAEMATLLEASKQFNYRVAAMIGEGMNAYRDVCMAKNFATKVCDRIVYDA
                     VQIHGGYGYCREYLVERLYRDSRLFSIGGGTYEIMNEVISKQMGF"
     gene            complement(21386..22252)
                     /locus_tag="PLW16_02550"
     CDS             complement(21386..22252)
                     /locus_tag="PLW16_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69818.1"
                     /translation="MAGKGSIFLNQGRRAVRCGGFLGARLRSGSGRPDYLMGVGDCML
                     LDFRKGFFAVADSSERNSSSSRAFMLRFAGLLEGLDGIGAPRSFDDAQQAALREEVER
                     QSEKILGEMSFTDSCTFTGVLILKSATGCRGLLLHTGDSLLLHYDLGAATALQVTRSN
                     FWMVGRTPRFFQVEYLSFSDESRLLLSTDGFTYLRTPPPERREDFIRGLFEEHPVEDI
                     PDILIDGYDAGDIAKDDLALISLRPAGLCFHDQRIILGGTTSHVERSFREGQTDGRYE
                     DLYTPVLQAGAA"
     gene            complement(22316..22768)
                     /locus_tag="PLW16_02555"
     CDS             complement(22316..22768)
                     /locus_tag="PLW16_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB69819.1"
                     /translation="MEKSYFEDYTVGEKFISPARTITETDITLFAAFTGDWHPLHTDV
                     EFAKKTFFGERIAHGMLSLCVGSALIFRLGPYVALPKSFIAFYGMDSVRFVGAVKIGD
                     TIHCEGEIMDLQAKDDKRGVIVSKNSIKNQRGEDVVIYTTRALVGRKP"
     gene            complement(22805..24607)
                     /locus_tag="PLW16_02560"
     CDS             complement(22805..24607)
                     /locus_tag="PLW16_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPB69820.1"
                     /translation="MGQNFYKRDDRDVKFVLKEHLGIKRLLEFEPYSAFTMEDFDMIL
                     DQAQKIAANDIAPTFQDGDREGCHFDQGKVTVPRSFHDCWNVFKEGSWFALPLKPDYG
                     GQGIPLIIAEAAQEFFMSANFAFGCFSGMGPGNGAMIENYGSQAIKDLFCAKMYDGTW
                     GGGMCLTEPAVGSDAHMVSTKAIPDGSNYKIQGTKIFITSGEHDLTENIVHLVIARIE
                     GAPAGAKGVSLFAVPKIWVNEDGSLGEPNDVACIGIEHKMGLNGSATCVMNFGENGRC
                     RGHLVGEAGMGLAYMFQMVNVARMAVGLESVAFGANIYANVLAYAKERVQGTPFGKGG
                     ARVRIVEHADVRRMLMNLKSLTEGMRALVYKTYFMEDLAKNSLDEAERKRAKGRLELF
                     TPLVKAYCSDRIFEMGREGIQILGGYGFTKEYPVEQYTRDCKIFSIWDGTNFIQSADL
                     VGRKLSMGGGAVFKGWIDEVTAFIGTHKSETAFSGELEILEEALQTVAGLALDYAKYL
                     SEGKLDLVPLTSTRFLDCAAEVAIAHLLLEQGMIALGTLEQGQCGGADQAFYRGKVET
                     VRYYCRNFLPQVFGRARIIRMEDTTAVEIPEDYL"
     gene            complement(24737..25354)
                     /locus_tag="PLW16_02565"
     CDS             complement(24737..25354)
                     /locus_tag="PLW16_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB69821.1"
                     /translation="MDEATTQKKDIILNAAIKVFAEKGYYSSRTLDISNEAGVAYGSL
                     YHYFRSKDDILMSIFQERWNLLIAKVRQVREQGSGPEEQLGMVIDFIFRSYRQNPDLL
                     KVMIMDVPRLDKFYNPENWKLYNLFFRGLMEIFHEGQEQGVFAADVSPILATIIMHGA
                     VDATIRQYVYNPEFNKEEFPVEEARGQIMMVLRRGFSKKVEGEKS"
     gene            complement(25509..26447)
                     /gene="mdh"
                     /locus_tag="PLW16_02570"
     CDS             complement(25509..26447)
                     /gene="mdh"
                     /locus_tag="PLW16_02570"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPB69822.1"
                     /translation="MQKKVTVVGAGNVGATAAQRLAEKALCEVCLVDIVEGVPQGKAL
                     DLTEAAPIERHDCRIVGTNRYADSAGSDIVIVTAGIARKPGMSRDDLLATNMKIMRQV
                     VTEAVRHSPHAILIVVSNPLDAMCHVALEASGFPRERVVGMAGVLDAARFRAFIAMEL
                     KVSADNVQAGVLGGHGDTMVPLPRYSTVAGVPITELIPEERIAALVERTRQGGAEIVQ
                     LLKTGSAYYAPASAAVEMTEAILRDRKKILPCAAYLDGEYGIRGLFMGVPVKLGAGGI
                     EQIIELKLTETETAALKQSAATVAELLAVMKRLGVS"
     gene            26804..28153
                     /gene="glmM"
                     /locus_tag="PLW16_02575"
     CDS             26804..28153
                     /gene="glmM"
                     /locus_tag="PLW16_02575"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPB69823.1"
                     /translation="MGKLFGTDGVRGVANQYPMTAEMALNIGRATALQFKRAGHAPKI
                     IIGKDTRVSGYMLENALVSGICSMGVDAILVGVLPTPGIAYLTNSMRVDAGIVISASH
                     NPFQDNGIKIFSGEGFKLPDEKEAAIEDMIFANNLDKLHPSPTELGKAYRMDDARGRY
                     IVFLKHTFPKEYTLEGMKIVLDCANGAAYRVAPETFLELGAEVVTLFDQPNGRNINAT
                     CGSQHPEGLAQEILRQKADVGFAFDGDGDRCIAVDETGTVLTGDQIIALCAYVMKKEG
                     TLTNNLVVRTVMSNIGLTVAFEKLGIDSIMTRVGDRYVLEEMQARGSAIGGEDSGHII
                     YLREHTTGDGILTALQVVAAMKKESKPLSELARVMTVFPQSLINVDTKSRPEIATVPQ
                     IMTVIREVEAGLGSQGRVLVRYSGTQNMCRVMVEGPTPEETDRACRQIADAVKAAIG"
     gene            28263..29291
                     /locus_tag="PLW16_02580"
     CDS             28263..29291
                     /locus_tag="PLW16_02580"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPB69824.1"
                     /translation="MPFTVIITRDFDQMSEVAAEIVIQRIQERLQEKASCILGLATGN
                     SPTGLYKHLAKAVNSGNIDAARIRTFNLDEYIGLPGENAQQRALHPEGYSFFMIQELF
                     GLLQRKFGATYVPWGTLVDQERLAAELADHPEDWSEQGEDRGRAIVIRADARSDYLKW
                     IRREILDAYREKIEADGGIDLHVIGVGGRGHVAFHESGIPFEDNTMLLVRLDDNTVAN
                     AVADGHFRSRKESPCYAVSMGAELVYRAGTVLLLANGTRKTAPVADSLLNDPTPLVPI
                     SYGQIYARRGGDLIYVLDRAAAARILEQPERLRERGIRLEDISADGAKVRVRELTFAR
                     DPEMGQMG"
     gene            complement(29295..30245)
                     /locus_tag="PLW16_02585"
     CDS             complement(29295..30245)
                     /locus_tag="PLW16_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPB69825.1"
                     /translation="MEKIRVGISTCLLGKHVRYDGGHQEDRYLTHTLSRYFDWVPVCP
                     EVEYGLGVPREAMRLVGDPERPRLVTIRTAVDHTDGMKRWARERLSALEREGLGGFVF
                     KSKSPSSGMAAVKVYPDTGGSPVKKGVGIFAAALMERFPLLPVEEDGRLHDPSLRETF
                     LERIFVYHRWRELWRDGGRMADLVRFHAEHKLLLLSHSPKHVTLLGRLVANPERRPLR
                     ALREAYLRGMMEGLKLPATTRKNTNVLDHMAGYFKKRLTADEKEELRNVIDRYHRGLV
                     PLVVPLTLLGHYVRRYDEPYLKRQVYLNPHPLELMLRNHV"
     gene            complement(30425..31123)
                     /locus_tag="PLW16_02590"
     CDS             complement(30425..31123)
                     /locus_tag="PLW16_02590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPB69826.1"
                     /translation="MAKIQVKFKDAVIKEVPLTQDILTIGRKEGNDLVIDNQAVSGSH
                     ARIVKEEQDFSVEDLNSLNGTFINGKKVSKYILNNGDVILIGNHTLEYFSEKPGEDQV
                     KKPALRGHSMDETMVLSPTEQKKILVSTEKLDVLGGFLIVQGVTEKKDYLLKDRITTI
                     GKDDGALIKLKGFFAPKVAALVNRRKDGYFINPSGGKDLKVNGKKVEQRCDLKDGDMV
                     EVGDLKMQFYLKES"
     gene            complement(31127..31942)
                     /locus_tag="PLW16_02595"
     CDS             complement(31127..31942)
                     /locus_tag="PLW16_02595"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPB69827.1"
                     /translation="MKVFELRAAGQTDVGRVRANNEDAFCLLPEQGLLAVADGMGGHA
                     SGEVASKMSMDLIRGYFADAGTGPSPQVGDYDEDRSELTNRLGSAVRLANMAVYEASE
                     SNAQWQGMGTTVVAALIRKNKLSIAHVGDSRLYLIRYGNIEQLTDDHSVVAEQVKRDL
                     ITREEADRSEIKNILTRALGTEADVEVDLEEMTLLADDVLVLCSDGLSNMVSDDDILT
                     IVLSTRDPALACQTLVKIANENGGRDNITVVVAYLANTGWLASFINFFRGRRR"
     gene            complement(31939..34416)
                     /locus_tag="PLW16_02600"
     CDS             complement(31939..34416)
                     /locus_tag="PLW16_02600"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPB69828.1"
                     /translation="MKKTKVFRTDVFIGVILTLLILGVYALRWSPVEFLEYKFYDLGI
                     NLREGPTASPVVVVAIDDSSINALGRWPWPRSHIARMIDLLQGYGAKVIGLNILYSER
                     DTNRGLQAIRDVTASIEAKPGLQSSASVAEIYATLKTAEKNLDHDAALSTAIAASQRV
                     VLPFFFVLGGPVLMDSAGMAEALARNSVSFASPPDLVTARELIPPVPEFSDPALALGH
                     INLAADSDGAVRNDCLLIDYGGRVFPSLSLQLSLEYLGYDLKDMVIGEALTFRRIRIP
                     IHEQGRMLISYSGSYEAYPFVEVLNNRVAPESFRNKIVIIAPTATGLGTMQVTPIGTT
                     MPSVLITANAISNILNNDHIVRPGWATLLEIGILILFGLFLALGIPHLKAGISALISS
                     VLLVAWLVTAAYFMLAEGIWLKVFYPTLLLLVGYTIVVSRGYLFTEKSKERIEADSVE
                     TNKMLGLSFQGQGMLDMAFEKFRKCPVEDEAVKELLYNLGLDFERKRMFNKAVAVYEH
                     VVTAGSFKDIEERIKKLKAVGDTVIFGAGGARKDTTVIVENAQTRPTLGRYEIMKELG
                     RGAMGTVYLGKDPKINREVAIKTLRYEEFDEEQLADIKSRFFREAEAAGKLAHPNIVT
                     IYDVGEDYEIAYMAMELLDGADLAKNCQKDQLLPRAEVVRIISSVAGALDYAHASGIV
                     HRDIKPANIMILNNGEIKVADFGIARVMASSKTQTGVIMGTPSYMSPEQIAGQKVDGA
                     SDLFSLGVVFFELLTGEKPFAGDSIATLIFNITTTPPPSVREMAPDLPEQFETIIGKL
                     LAKDKSQRYQHGKELVDDLQELMKVVS"
     gene            complement(34664..35131)
                     /gene="bcp"
                     /locus_tag="PLW16_02605"
     CDS             complement(34664..35131)
                     /gene="bcp"
                     /locus_tag="PLW16_02605"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(34994..34996),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPB69829.1"
                     /translation="MAKLNVGDAAPAFSLRDAADIMVKRSDFKGRKLLVYFYPKAGTS
                     GUTKQAEAVRDARAELDALGAGAVGISPDSVAAQKTFSDRLKLSFPLLSDPDHAVAED
                     YGVWQEKSLYGKTYFGIVRSAFLIDADGKILAVWYKISPADTVPKVLAALRSG"
     gene            complement(35185..36855)
                     /locus_tag="PLW16_02610"
     CDS             complement(35185..36855)
                     /locus_tag="PLW16_02610"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPB69830.1"
                     /translation="MGTSEKPSVPERLRPIRDIASTIGLREEDLEYYGRYKAKVRLEA
                     IRRFPRRDDSNLILVSAMTPTPAGEGKTTVSIGLAQALARLGKSTIAALREPSLGPVF
                     GIKGGATGGGLSRVLPVDDINLHFTNDFPAVESAHNLLSALVDNAVFHGNPLNIDPRK
                     ITWRRVVDMNDRSLRDIVIGLGGSTNGVPRETGFDIVPSSEIMAILCLSRSYAELKDK
                     IRKILVGFTYDDKPVIAGDLKVEGAVTALLKYALLPNLVQTTEGVPAIIHGGPFANIA
                     QGTNSVMGTDLALRLADYVVTEAGFGFDLGAEKFFDIVAPYGELNPRIVVLVATVRAL
                     KYHAGVAREDLNAPNARAAVLGMANLRKHYGNIDKFNVPCIIALNRFPSDTDEEVRAV
                     VDAAEREGMNIAPADIFRLGGEGGLDLAEKTVRLIAGATGTFRTLYDWEQPVEDKIFT
                     IASEIYGAVSIDYRPLAKRNLDLIRRFGFDKLPVCIAKTQQSLSDNPSLLGMPRDFIV
                     TVREIKIASGAGFLIPITGEILRMPGLSKSPAAYHIDIDDAGTISGIV"
     gene            complement(36858..37397)
                     /locus_tag="PLW16_02615"
     CDS             complement(36858..37397)
                     /locus_tag="PLW16_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB69831.1"
                     /translation="MRSYVVFSSMGPDRPGLAKEFAEFFTARGINIERARGCVLGGEF
                     GMIILTSGKTDDIERLIGDLDTLRKKTGLDVYVRKTKAPSHREVLPSIPYKLIATAID
                     RPGIIHQISKTFQQRGISIDDIATNVDNNPVTGANVFQMICYFSLPPAVKILELKNDL
                     NRISDEYNIDIRFDAVINK"
     gene            37656..38651
                     /locus_tag="PLW16_02620"
     CDS             37656..38651
                     /locus_tag="PLW16_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB69832.1"
                     /translation="MKASLKLNRRGFLTATGGAALIGAASALGTPREAMAGRGRLATL
                     IDLTRCNGCDGRDVAACVSACKTAKAGVVPPPVDPIPVPFPTRKVEDWSKRQAVSDRL
                     TPYNLIYIQKAEVDTGGRKTTVFIPRRCMHCDNPACATLCPFSSNHKFENGAVVIDQD
                     TCFGGAKCKTVCPWEIPQRQSGIGIYLKVLPTLMGNGVMYKCDLCNDLIEAGKTPACI
                     AACPRQAMAIGPREEIFARAEALAKRIGGHLYGKEENGGTSTLYISPVPFADLNAAVQ
                     TGPGRPHLQPVARRMAGSDGLGKVVLAAPALGVAAGIVGVFAAISRRKDRSGKEE"
     gene            38655..39512
                     /locus_tag="PLW16_02625"
     CDS             38655..39512
                     /locus_tag="PLW16_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPB69833.1"
                     /translation="MSQDIREAENRVVRHSGLEMAEHWAIALSGLVLLLSGMFELPMA
                     ARYRITTLPGLAWSGDFITSIYVHYTASVIFIAAACFHLAYHALRGEAGLMPKKGDLR
                     ASIAVIKTFFGKGEEPPFGKYLPEQRLAYLGMAVIIAVLILSGLVKTYKNVFAPDLDY
                     TLVLWATWLHNAFFVLFILAFVAHIGALLLRPNWPMIRGIFTGTVRLDYARRRHPLWI
                     ATLEPPPAPPEAEEEPPAPPPVPPEATPGMTPETVATETETALPTTPPEGEGVVAPPA
                     DEPDSATRT"
     gene            complement(39582..40067)
                     /locus_tag="PLW16_02630"
     CDS             complement(39582..40067)
                     /locus_tag="PLW16_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HPB69834.1"
                     /translation="MRTTLIKIDGEFRRYWKHYVLQSLLATLSVFAVLYFLSLQHAVI
                     IASIGASAFIVFAMPDYVTAQARNVIGGQVVGLFCGFLFSLIPHPVMFSSILIYSLAV
                     GASIFVMVVTDTEHPPAAGTALGVAMTGISLHVLIAVVLSIGLLSLIRWLFKPYLRDL
                     T"
     gene            40329..43307
                     /locus_tag="PLW16_02635"
     CDS             40329..43307
                     /locus_tag="PLW16_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPB69835.1"
                     /translation="MLNRIEIGFREGIRDALGEKIRRRIVEHLGLAVDRVRTVDVYTI
                     EGDLTTAELEAAAAGPLSDPIIQTWSVNRALADRFDWLVEVGFRPGVTDNVGKTAGEA
                     IRLLLGRADAVFVRVYTSRQYILNGPLGRAEAERIAADLLANDLIDRTHIFDGKTWDP
                     ARGVAPCAPKVAGEDRPRLEEIDLDVDDAALLGISSARVLALNLEEMRKLRDHLKDPD
                     VLRERARVGLGARMTDAELECLAQTWSEHCKHKIFNARISYDDGTGRVRVIDSLFDTY
                     VKGATRAVRARMGESDWCLSVFVDNAGVIRFDEHWNLVFKVETHNSPSALDPYGGALT
                     GIVGVNRDPFGTGRGAKLIFNTDVFCFAPPDYAQPLPPRIMHPRRIYEGVREGVEHGG
                     NKSGIPTVNGCLVFDDRYLGKPLVYCGTGAVMPARLDGQPTHVKEILPGDRIVMTGGR
                     IGKDGIHGATFSSEELHESSPVTAVQIGDPITQKKMTDFLLVARDRNLYRCLTDNGAG
                     GLSSSVGETAQLAGGCDLDLAKAPLKYAGLDPWEILISESQERMTVAVPPEKIGAFLA
                     LAAKMDVEATDLGCYTDSGRFHIRYGDKTVAFLDMDFLHDGVPQLKLSACWTPPVHAE
                     PDFPEPADLGTALKAMLARLNICSKESVVRQYDHEVQGGSVVKPLVGAANDGPSDAAV
                     IRPFLDSFAGVVVANGICPRYSDLDAYQMAAAAIDEAVRNAVAVGGTLDHLAGLDNFC
                     WCDPVESEKTPDGAYKLAQLVRANEALYDVTTCYGVPCISGKDSMKNDYAIGNTKISI
                     PPTLLFSVFGRIDDVRRAVTMDAKKPGDLVYILGMTYNELGGSEWYALNGFVGNTVPR
                     VRPETAKARYDVLARAIRLGLVASCHDCADGGLGVALAETAFAGGMGMDVDLAKVPAE
                     AVGRNDVLIFSETPSRFLVTVSPDRESAFIEQFRGQTAARIGTVTTDGRLRLRGLRGE
                     ALLSEEIGALKAAWQRPLNF"
     gene            43402..44247
                     /locus_tag="PLW16_02640"
     CDS             43402..44247
                     /locus_tag="PLW16_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPB69836.1"
                     /translation="MAPTVKCIVLTGNGTNCEMEMAHACRLAGAEVVEIVHLSELLCG
                     EKRLEDYHFLNLPGGFLDGDDLGSAKAGANRILHAKIRGRAGATNAETRLYDQFSRFI
                     EDGKLILGVCNGFQLLVKLGVLPAFDAAERAQTVTLTYNDSGRFEDRWVHLKVDPASP
                     CVYTEGLTGLYLPVRHGEGKFIPGSAEVLARLHREHCIALQYSTADTATATLDYPANP
                     NGSVDAVAGICSASGRIFGMMPHPEAYLHRTNHPRWTREDLPETGMGLALFRNAVSFI
                     KRQRL"
     gene            44305..44721
                     /gene="rnk"
                     /locus_tag="PLW16_02645"
     CDS             44305..44721
                     /gene="rnk"
                     /locus_tag="PLW16_02645"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPB69837.1"
                     /translation="MAERPVYMTSFDMERLESLIQTHRSSSPKRKAQIDRLEKELDRA
                     VIVDPKDIPADVVTMNTKLRLRDETSGEEMVLSLVFPADANLEKNRVSVLAPIGTALL
                     GYRVGDIIEWEVPSGTKTFRITETLYQPEASGHYDV"
     gene            complement(44806..46413)
                     /locus_tag="PLW16_02650"
     CDS             complement(44806..46413)
                     /locus_tag="PLW16_02650"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPB69838.1"
                     /translation="MKTKFIFITGGVLSSLGKGLAAASIAALLECRGLRVSNQKLDPY
                     INVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFCSTRMSKANNLTTGQVYFSVITK
                     ERRGDYLGKTVQVIPHITNEIKDFIRGTAAGYDVAIVEIGGTVGDIESLPFLEAIRQF
                     RNEVGRQNAIFIHLTWVPLIKTAGEVKTKPTQHSVKALREIGIQPDILLCRSEQSLTE
                     DIKAKISLFCNVEVGAVFAARDVSCIYEVPLNYHREGVDRQIVDLLNIWTGQPHLEAW
                     EDVVNRMTHPTHEVTIAIVGKYVNLTDSYKSLNEALIHGGIANDCRVQLRFVDAERIE
                     KDGAGHLLDGAGGVLVPGGFGDRGIEGMILAAQCARTRRIPYFGICLGMQMAVVEYAR
                     HVCGLSGANSSEFDAATLHPVIDLLPEQRAVKEMGASMRLGAYPCVLDAESLAFAAYG
                     EGEISERHRHRYEFNNDFKDLLVQKGLNITGVSPDGRLAEIVELGDHPWFLGCQFHPE
                     FKSRPTAPHPLFSRFIAAALAHKGITD"
     gene            complement(46410..50231)
                     /locus_tag="PLW16_02655"
     CDS             complement(46410..50231)
                     /locus_tag="PLW16_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB69839.1"
                     /translation="MITRIVRRLKAWRVYVGRNPQGVSAPAIILLPVFNGVFFCGFAG
                     ILAIRSHEPLEDDARSPGRDVLPRLTRIALELKGNGLEAVLEGSVSPGEYLAPEAGDR
                     GPDGQSPAGLEPLVRCLKETRFFQALFFDSDRVHALSRLVADLKAFLADEERLLEDAA
                     GRFRTETLEIVNSRLVSLKDAVWSLEKDVLENVGRIAALAGAGRDVDVAAFRKLHYLN
                     FLLNGLDRLEVRGRDSAGIQISFSLSDPEDWRRIRRELKARNLDEAFRKRSAPGDLIP
                     GSISVFPPDGDTDPAGQAGRTVAFTFKTASIIGELGRNVRDLRQAIAGDEILQLVVRH
                     PSAAELSMAHTRWASVGSITVENCHPVNNFAPSGSDAGTERTRPRPGRSVGSISVVLN
                     GDIDNYAALRDALEARGETVAAEVTTDTKIIPLEIERYLRAGDDLTEAFRRAVAGFEG
                     SHAIAMTADCAPGKVFLALRGSGQSLYVGLAPDRYLFSSELYGLVEGTPTFLKMEGEA
                     PSRGRGAAMPAGQIVILDRESPGGLAGLRALRYDGAPFPLTEDDLQRAEITTRDIDRG
                     AYPHYFLKEIEESVLSVRKTLLGKYRRTADAKTGPEVRFNLGVDILPQPLRGRLERGE
                     LDRIVVIGHGTAAVAGQAVADALARDLKGSRIVVETRLASELSGFSLEDELASALVIP
                     ITQSGTTTDTNRAVALARERGAAVIAIVNRRQSDITTKADGVFYTSDGRDIEMSVAST
                     KAFYSQIVAGRVLSLCLAQLLGTLTDAAIDRELAVLERAPEMMRRVLERKDEIRAAVD
                     RLAIRKSSWAVVGSGPNKAAADEVRIKLSELCYKTISTDVVENKKHIDLSAEPLILVC
                     AAGSPEAVMGDIVKDVAIFKAHKATVVVFGDEGDDRFDDTADAVIPIPRAPWPLPVIL
                     NTVAGHLWGYFAARRIDADALFLREFRSRLNRQMAQGRREGQALFEMMADRAYRQMVR
                     AFAAGFQERRRAGSFALLSVGTIADLVLLLKYALGKLPLDDFWEDFPGERETASPVDL
                     LDIALGHAVDELSRPIDAIRHQAKTVTVGTSRKELFHRGVIFDLLKELGFSLKGLVSK
                     NIPDMVRIQKAVAAVTGYTLYDIEPLDGEGRPDERSLIRIRQRGGVALNMASRAEGAA
                     ALMGTKRTILNTGKIYAGYGKSDGAPLVMIPLWGETRGVRHLLLVHVRFADALSTQEK
                     REILGVRYQDLRNLVNEYNLPWEDRYLEDLPIGVLLDEAVEVVAGQIRARVGPAAGAG
                     KRQTFRQEESL"
     gene            complement(50493..51743)
                     /locus_tag="PLW16_02660"
     CDS             complement(50493..51743)
                     /locus_tag="PLW16_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPB69840.1"
                     /translation="MQEQNRFSPQVERLLARGVRIPLPQTVEIGDEVDPGRISGDGVV
                     LHAGTRISGARTLIAAGCRLGQEAPVSLNDCLLGPGVELKGGFFNSSVFLEKANLASG
                     AQVREGCLLEEEANGSHTVGLKQTLLFPFVTLGSLINFCDCLMAGGTSRKNHSEVGSS
                     YIHFNYTPQQDKATASLLGDVPRGVMLKAPPIFLGGQGGLVGPARIGYGTVIAAGTVW
                     RGDCPEGGALLSATGRNVRRPFTPGLYGDVRRRVLNNLHYIANLLALRQWYLHVRKPF
                     FRGQDLGAALFDGAMQVLELALVERIARFEALAEKMETSIRCLESRVDETACADIVRP
                     QRALLGGWPRLKAFLTSGEEASTAPERREAFVKRIAEAAGTSGYLAVVQSLEAATAAA
                     GTAWLQDIVDTVVDRSLAILPECR"
     gene            complement(51792..52082)
                     /locus_tag="PLW16_02665"
     CDS             complement(51792..52082)
                     /locus_tag="PLW16_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB69841.1"
                     /translation="MNTLRVAKSSEAAKLPSRVENLLEKKMQDMTLLLCDEGNGRSKL
                     YEEVMAMVERSLFKIALERSNHVKSAAATYLGMNRNTFQNRMNKLGMNGQPD"
     gene            complement(52126..52605)
                     /locus_tag="PLW16_02670"
     CDS             complement(52126..52605)
                     /locus_tag="PLW16_02670"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB69842.1"
                     /translation="MIPLTAVLILIIGLLALSFFAGTPTERIFLALVLIPLALVWSES
                     LSRRVSIGADGITLRKFLRPKTLRWEDISHVGCLVLRKKVYLLLTTGRGFHILSNEYE
                     RFADLARGIATHLDREKIEAEVLQQAENPTRNPANIAAAWVAAAVLAVLVYLKLFPA"
     gene            complement(52638..53141)
                     /gene="moaC"
                     /locus_tag="PLW16_02675"
     CDS             complement(52638..53141)
                     /gene="moaC"
                     /locus_tag="PLW16_02675"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPB69843.1"
                     /translation="MPTLSHLDEQGRARMVDVSVKAPTLRQATAGASIRMRRETAALI
                     VAGQMPKGDVFQVARIAGIMAAKRTGDLIPLCHSLELTDVDLSFAVDAREGTVSVEAT
                     AKTIGRTGVEMEALTAVSVAVLTIYDMCKAVDRGMVIGEVMLLHKRGGKSGEYRRGGA
                     GKGSDRE"
     gene            53437..54966
                     /locus_tag="PLW16_02680"
     CDS             53437..54966
                     /locus_tag="PLW16_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPB69844.1"
                     /translation="MIVKVFSSAIIGVDAYPVAVEVDISPGLPQFSTVGLPDVAVRES
                     KDRIKAAIKNSGYPFPKTHVTVNLAPADIKKEGTGFDLPMAVGILAADGTLGPQPAQD
                     YLLLGELSLDGSVKGVPGVLSAAFQARASGKKGIIVPVENAREAAMVQDVGVMPVGNL
                     AEVVEFLSGRRAIEPVVVDAAAVFSQSHRYPFDFKDIRGQEQAKRALEVAAAGSHNVL
                     MIGPPGAGKTMLAQRLPTILPDLTFPEAVETTKIYSVAGLLDRKQAFLATRPFRAPHH
                     SISDAGMVGGGHVPHPGEISLAHQGVLFLDELPEFRKNTLESLRQPLEDGTVTISRSL
                     MRTTYPARFMLIAAMNPCACGFYGDPRRACRCSPRQIRQYQGRISGPLLDRIDIHIDV
                     PAVRYRELAARQTAEPSAAIKARIEAARRCQRERLPDHPLFTNTRLTDPQLKAFCALD
                     TESQRLLEMAVDRLGLSARAYTRILKVARTIADLESEADIRSPHVAEAIHYRTLDRAL
                     I"
     gene            55031..56104
                     /locus_tag="PLW16_02685"
     CDS             55031..56104
                     /locus_tag="PLW16_02685"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPB69845.1"
                     /translation="MEITIRELPPEKRKAKPADESKLGFGKFFTDHMFTLKYDTALGW
                     HDALIGPYQPLSLDPTAMCLHYGQEIFEGLKAYRGQGGAIYLFRPEENIRRMNASAER
                     LCMPPVDPALFLDALKELVRVEKDWIPHSRGASLYIRPTMIATEPALGVHPAGAYLLF
                     IVVGPVGAYYAEGFAPTKIFVSEDYVRAVRGGMGNCKAAGNYAASLYASRIASDMGYT
                     QVLWLDAIERKYAEEVGTSNIFFVIGDELVTPPLAGSILPGVTRNSILQIARHWGCRV
                     VERPVAIAEVIEAAGSGVLKEAFASGTAAIVSPIGQIYYQGKEHVINGGKTGALTERL
                     YNEILQIQYGEKDDPFGWRIQIA"
     gene            56504..57142
                     /locus_tag="PLW16_02690"
     CDS             56504..57142
                     /locus_tag="PLW16_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPB69846.1"
                     /translation="MTVLSLIGASPAGADLPPLSEVIAGIQRQYDGMTDLRARFVQEI
                     TLSAMQRTDREEGTLYWKKPQRMLWHYHKPRVKKLIINQRQTWLYIPEDQAAYTQKTG
                     ALLKSQTAVRLFSGFSRLQEDFEIRFAQPSGKDREGNYLLLLTPKGRDTGASDIVVTV
                     DPERHLVIRCRFLDAYGNVTRLSLTQVQTDRRLPDRLFRFEPPPGVDVLPLP"
     gene            57182..59269
                     /locus_tag="PLW16_02695"
     CDS             57182..59269
                     /locus_tag="PLW16_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPB69847.1"
                     /translation="MMNIALPPWTEPGANESFLKREWLETNGRGGYASSTLLNCHTRK
                     YHGLLVANLASPPGRYVLLSKFEDSLRLRDREIYLSCHQYPGTLFPDEVLLREFILDG
                     YPSFLYTASLPGPGTTAGIKGDTPAGGTIRLRKSVLMIAGEDAVLVHYDLEAAPAGAV
                     LRLRPFLAYRGRHELSRRNPFLRAEVQPAPNGFFMNPYQGMPPLYLQTNRKGRFIEAP
                     AWYENFEYVREQERGYDGHEDLFTPGILEVPIRTGQAILVRAATTPGNTALHRLWQRE
                     ADGRRTATTEGKVSEPGRIAPPPGPEDSSAGGLAAEDCSCRERLFRAARRFLVTTPAG
                     RPAVIAGYPWFGDWGRDTLIALPGIAFCRGRFEEGAAILGALGDHERDGLLPNYFTAD
                     ESDPPYNTVDASLWFFWAVQQYLLYGGDPERVRLRFWPVMTRILTHFLDGTRLNIGMD
                     DRGLLHAGDGRDALTWMDAQVGGRPVTPRDGCAVEINALWYNALAFSEQLSHRFGKQD
                     IDLEGMLRKIRQSFQDTFWLESEHYLGDVYAGNRLDRAIRPNQILAVSLPFSPLDPFK
                     WKAVVHTVTDHLLTPCGLRTLSPEDPAYRGVYGGDPAARDGAYHQGTVWPWLLGHFGE
                     ATLRVAENRHAARTVLLRHLRSFLQAHLPEAGVGGISEIFSGEAPHRPDGCIDQAWSV
                     GELIRLYDLLARG"
     gene            complement(59395..59841)
                     /locus_tag="PLW16_02700"
     CDS             complement(59395..59841)
                     /locus_tag="PLW16_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69848.1"
                     /translation="MRAFADKLIDILERHTEEVARQWSKAVRSNPRTPFYHALSEEDY
                     VQWGISFYENLRKLYFSARPYPEVDEFFSRLGREMFNKGIPLEEAVYTLILLRRHIWL
                     FADFNVVFVTTLDMHQAVESINRTIQMFDQGIFVVTRKYGELTKSR"
     gene            complement(60029..60853)
                     /locus_tag="PLW16_02705"
     CDS             complement(60029..60853)
                     /locus_tag="PLW16_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB69849.1"
                     /translation="MSFRPAWWLAVLARIWPLTWISARMTRWPVVGRVVAWGSLPLFA
                     GKNLNITYLPVNEEVGGTANTLLPRQVVEALIRRSAHRVIINRCTCRDAKSCRRHPVT
                     MGCTLLGEGTKEIDPRIARHATVEEALAHLDDALREGLIPMTGRVRIDNFIWGVKDRG
                     KLLTICYCCRCCCTIMNSGRYLPAEAQAAIHPLAGLTISVDPDLCVRCGACVEECFMG
                     AIRLEDGEIVHDAVLCKGCGRCAAVCPQAAIRVVVADVNRAVAELMGRIDSLVRYE"
     gene            61188..61535
                     /locus_tag="PLW16_02710"
     CDS             61188..61535
                     /locus_tag="PLW16_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPB69850.1"
                     /translation="MYKTVVLLFFSNLFMTFAWYGHLKYMNGRPLLVVILLSWGIALF
                     EYTLQVPANRIGFHYYNLGQLKVIQEIITMTVFAGFVFLVMKQPLKWDYLWAGLCLVG
                     AAYFMFRGGLPPA"
     gene            61862..63478
                     /locus_tag="PLW16_02715"
     CDS             61862..63478
                     /locus_tag="PLW16_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPB69851.1"
                     /translation="MEVIETRIDPRSEAYRQNYEAMAALVTTLKEELRIAREDRSPQA
                     RNRLASQGKMPMREKLDLLLDRNTPFLEIAPLAARGLYDGKIHGAGLVSGIGVVGGRE
                     VLITGNDAMIKGGATYPLTVKKSLRCQTIAMENRLPAITLIDSAGGYLQLQSEVFPDL
                     DDGGRLFYNQAIMSKMGVPQITAVMGLCTAGGAYVPAMSDETVHVKGTGAIFLGGPPL
                     VKAATGEEVTADELGGADLHCRESGVSDYYAEDDRHAIAILRNIVETLPRTEKARLPM
                     HEPAPPLYDPKEIYGIVSRELKVPYDVRELIARIVDRSEFLEFKELYAPTLVCGWAYL
                     HGYPVGILGNNGVLFPDSALKGTQFIQLCDRRQIPLIFLQNINGFIIGSEYERLGITK
                     NGHKMVNAVATATVPKFTVIVGASFGAGNYAMCGRAYSPRFLWMWPNAEIAVMGGEQA
                     AGVLVTLRNDQLAKAGRPPLTEEEAAQIRDPVVAAARTESNAYYSTAQLWDDGILDPA
                     DTRDVLGLAISAALNAPIRDDVFGYGIFRM"
     gene            63520..65007
                     /locus_tag="PLW16_02720"
     CDS             63520..65007
                     /locus_tag="PLW16_02720"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPB69852.1"
                     /translation="MFKKILVANRGEIARRILHTCREMGIGTVAVYSDADRKAVHVLD
                     ADEAVHLGAADPSESYLHIDRIIAAAKETGAQAVHPGYGFLAENGDFAERCEREGLVF
                     IGPPARVIRALGDKTVARETMIRNGVPVIPGMNRPESDPDTLAKEADLLGYPVLIKAA
                     AGGGGKGMRIVQARDALHEACVSAASEASAAFGNGAIYLEKYLARPRHIEFQILADGL
                     GNMVHLLERECSIQRRHQKIIEETPSPALTPELRERMGQAAVAAARASGYVNAGTVEF
                     LLDPDGRFYFLEVNTRLQVEHPITEMVTGIDVVRRQIEIAAGLPLNLTQQEIRGRGHA
                     IECRIYAEDPEQGFFPSPGTIVFMQEPSGPGIRNDCGIYAGFQVPVEYDPILSKLVAH
                     GEDRDAAIARMLRALERYVILGIRTAIPFLMDVLRSDAFRAGETFTDFVAAHFSDWKP
                     ARTETDLARLAYIADDLGGRRETKATGLPAGEHPSPWQRLGNWQR"
     gene            65053..65586
                     /locus_tag="PLW16_02725"
     CDS             65053..65586
                     /locus_tag="PLW16_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69853.1"
                     /translation="MQYRFKTDEDAAVWTVDPEGTDRLTVTTGTQTLHVRHGAVSPHH
                     LHLAVDGLGVNTFVLRGDGEKTVVIRGVPYPVQDADFAERKTKKKADRENLPQVITPP
                     MPSVVVRIPVSEGDRVCKGQAVIVLTAMKMETTLTSPFPGRVTKVNVAAGEKVMPGRI
                     LVDIEKEAETDAPDEGR"
     gene            65632..66402
                     /locus_tag="PLW16_02730"
     CDS             65632..66402
                     /locus_tag="PLW16_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB69854.1"
                     /translation="MDERHLLYRVEDGIGTITINREAQRNAITPEAIRLFHVSLDEAE
                     QDERVRVLTVTGGGDKAFCTGAQLSGATTTEGRDLFADYAALLDKLVRFPKPTVARVK
                     GFCLAGGMGFMLACDIVIASDDARFGTPEVNVGLWPMMIGALIFRNVLQKKAMEMILL
                     GERMTAAQAMAMGLVTRVVPAERLDEEVDRVLKALASKSPIGMRIGKQAFYGMANLPL
                     PDALNYLSEKLKEVAATEDAMEGIVAFMEKRPPVFKGR"
     gene            66430..68223
                     /locus_tag="PLW16_02735"
     CDS             66430..68223
                     /locus_tag="PLW16_02735"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HPB69855.1"
                     /translation="MGATGQRGRDRVIIANCSGYFGDRLAAAREMVQGGPIDVLTGDY
                     LAELTMAILFAQKMKKPETGYVPTFLRQMEEVMGECLDKGIRVVSNAGGLNPRGLAAE
                     LKKIADALGLHPKIACIEGDDLMGRLADLQAQGEAFIHLDKGIGLKDAGAMPISANAY
                     LGGWGVAEALERGADIVVGGRLADAAVVMGPAAWWFGWRRDDWDRLAGAAVAGHIIEC
                     GAQATGGNYSFIDEVPSFLKVGYPIAEIFADGAFVITKHPGTGGLVSVDTVKAQLLYE
                     VRDPKYLTPDVVARFDTIGISQEGPDRTRIAGVKGEPPPATTKVCINNAGNYRNSMTV
                     VLTGLDIEKKAKIFEDALFDALGGRGQFAVEHVQLIRSDKEDPQTNDEAFAYLRLSVM
                     DPDRKKVAKFSAKIVELGLANIPGFTATAPPTKGGPAIVHWPTLIATKHIRQTVLLGD
                     ETWTVESVPPDPSAPAPEAPKIDLPPAPRGKTIRMPLGRVFATRSGDKGGNANLGVWA
                     KTPEAFAFLLEFLTTEKLKELLRDLSDFEMERHEFPNLLAVNFYIKGVLGEGVAASVR
                     SDPQAKTLGEYLRARVIEMPESIVSRTNAGR"
     gene            68300..70006
                     /locus_tag="PLW16_02740"
     CDS             68300..70006
                     /locus_tag="PLW16_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB69856.1"
                     /translation="MSAYDAKPWLKSYDPWVTPEMTIPDETYVDLLEKAIAHDPDRAA
                     FHFLGTTCTYRNLDRFSGRVADYLAARGCGRGDVVGINLPNTPQYLIALAGILRAGCV
                     ASGVSPLLTPREMAHQINDSSLKALITLDAIFEQRVQKIGNEIPSVKHCLVTNITDFL
                     PAYKRVLAKALKKVPVGRMAPIAGKEVLPFMDVLRKDQPPRPGIKIDPGDPCLIQYTG
                     GTTGLPKGAVLTHRNMVANITQAVTWFNLKMGGGVACSGFPFFHQAGLYFGLITMSAA
                     YTQCLIPDPRNTDHVCAEIAKYRPAILLHVPSLYQMLMDNPAFAKIDFSRCEVCISGA
                     APFSKEAITALEAIVGRGKVVEILGMTEASPVITMNPLRGRKKIGSVGIPLQSTRLKL
                     VDVETGTLEVPVGEVGEIIVHGPQVMKGYHNKPEDTAKTLRPFQNETWLYTGDVARMD
                     EDGYFYIADRTKDMIIVGGFKVFSREVEEVLSQHPAVQFCAVVGMTDPKRPDSQLVKA
                     VIQLAGEAAGSDAEAVRKDLLDHCRENLTPYKVPKVIEIMAAIPLTAVGKVDKKALRV
                     KG"
     gene            70069..71205
                     /locus_tag="PLW16_02745"
     CDS             70069..71205
                     /locus_tag="PLW16_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69857.1"
                     /translation="MYFNETHEAFRASVRRFIDREINPYMDEWEEKSVPLHDLFKKMG
                     ELGFLGIRYDPKYGGQGLDYWYETVLLEEAGHIRGFGVSTAIAVQTNMATPAIDAFGS
                     EYLKDKYLRGAIAGDLVAAIAVTEPDAGSDVFAIKTRAVRKGDHYLLNGSKTYITNGT
                     QADFLTLLAKTGDDGGYHSFGLFVVPTDLPGFVVSKKLDKLGLRSSDTAELFFDNVAV
                     PVENLIGDEGEGFIYQMKQFQHERFTLLPGVHVCVQDMIEMTVDYLKQRIVFGKPLIT
                     KQVLRHRLAGWLMKNECLKQLTYHIVRMKMDGRDVTREISMGKLFAGQLIAEVASGCL
                     QMFGGMGFMNETLISRYFRDSRIMSIGAGADEVMYEIIARLEGF"
     gene            71210..71914
                     /locus_tag="PLW16_02750"
     CDS             71210..71914
                     /locus_tag="PLW16_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB69858.1"
                     /translation="MTKTGKTGDDAVPRRSMRRLREKENRSNTLLKAAETLFGQKGYH
                     QTSIEEIADLAEVSTGAVYFYFKNKEELLIKLMQEIGHDLRKALGEEYRKSEFSLDSF
                     RNVAFAFLGDFCLRHPEKIAIFFRESVGQSVEVEEQRKRVFVKLTSDIKDAIIRISRG
                     QGRNFATERSPELIAVCIVGIYERIACHYLLWQDHPTEVLRMAEESAAFMLGGVNSLF
                     APDAPKTAKHEKKAPK"
     gene            complement(72002..72550)
                     /locus_tag="PLW16_02755"
     CDS             complement(72002..72550)
                     /locus_tag="PLW16_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HPB69859.1"
                     /translation="MRIAVFSDTHGNKNDMRDVVRTFGPLDLVLHLGDGVRDGGLVAH
                     EADLPFQGVQGNEDYGSDLPGKLVLDLDGWRFLLAHGHHMNINPYHPAAVWQQDLQEM
                     ADWAKGQAADVLLFGHAHKPVLQKENGVVLCNPGDQYIGATTGASFALVVLEDATAHF
                     RIFRKNGQGVWHPLMELRPTRA"
     gene            complement(72556..73551)
                     /locus_tag="PLW16_02760"
     CDS             complement(72556..73551)
                     /locus_tag="PLW16_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB69860.1"
                     /translation="MNSADLYQELAQKVGMAHSAIVPDLWRMICSEEEARIVNAMPGT
                     ADELAETFGKPVVEMQALLDELYHRGVAFDYVKDGRQFYRMPRHIIQFHDATILWPEA
                     PQALLDLWVKFTEEEYPSLPALVTQAGLPSFFRVIPVNEAIASKSEVLPYEDAAKILE
                     NANSLAVTKCTCRFVMKKCNRLLEACIQLNRGADYAIKRGTGRRIDVAEGKEILKQAR
                     AAGLVHMTENKAGIGNVICNCCDCCCIGIPFAKNPATRGVIATSRYRADVDAGSCTAC
                     GLCIEVCPVEAISENGAGAVTVDPALCIGCGLCAFECPVAAIGLKQVHPASFIPA"
     gene            73704..>73965
                     /locus_tag="PLW16_02765"
     CDS             73704..>73965
                     /locus_tag="PLW16_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HPB69861.1"
                     /translation="MEDDTGLALNPWREIYPEKFASEERVFGRIRRGNRIFISTGCGE
                     PQYLVRALIQYVESHPTAFFDTEVLHVWTLGVAPYTDVKFKEN"
BASE COUNT        13426 a        24191 c        22562 g        13786 t
ORIGIN      
        1 cgcaggcgcc gagacaggcg ccggaggcca gacggtagag aaaattcttc ttctggtctt
       61 ccgttccggc caaaagcagc ggatcgctcg ccagggcctg cgtggtcagg atgacggacg
      121 tggcggcgca gacccgcgcg atttcctcga cgaccaggac catcgcgagc atccccgcac
      181 cggccccgcc gtacgcttcc gggatctgga cacccaggag gccgttctcg gccaggacct
      241 tcaccgactc cttcggaaag gtttccttct cgtcaatctc ggcggcgatg ggggcaaact
      301 tctcttcggc aatccgccgc accgtatccc gcaacatcgc ctgatcttca ttcaaaataa
      361 aatccatatc catcctccat cgcccgcaca gagccgcggg ctcctcggtt gcctctaaca
      421 ggccattgaa aaacgctcat ccgcggcgtt gcgctgtaaa tctcatcgct cgacttattt
      481 ctgtatacgt ctcgctcttc ggtttttgcg cgccttgcat ctgaacattt ttgaacagcc
      541 tgcccaatcc gggtgttttt cgacaccctg ctaaaggggc ctcccctctc ccaccgggga
      601 atgccccgcc ctacctcacg atatcgaaat acttgatgtc ctgaatgttc ccctgcattt
      661 ccgcggtgtc cttgaaaatc gcccgcacct tcatgccgac cttgatcttg gagaggtcca
      721 tctcgttgat gatgtgggag aagatgttgt ccgccccgtc cagcttgatg tagccgtagg
      781 tgtaggggat cggccgctgc gcgccggtgt tcggatcgat gaacgggtag ttcaccaccg
      841 aaaaggccgt gatgatgccg gtgtcggaca gggtcaccag ttccgtgagt tcccggaaac
      901 agggcccgca cacccgccgc ggtggaacgt agaccttgcc gcacccgtcg caccggatgc
      961 cgacgaaccg cctgttctgt ttgatttcct gaaaaaactt gctgccgtac agcccgatgg
     1021 accattcgta aggcatcgga taggcaaaaa ccgacacgag tcgttcgctt tcgacttgat
     1081 gagccatgat cactccccct cttcctatgg tctctcggtg ctcaggatga cgacgtccga
     1141 ccagaagcaa ccgccgaatc ccgtggccag ggcgttcttc acgtcgggca cctgccgctt
     1201 gccgcccttg cccatgacct gaaccgccgc ctcggccacc cgaagcatgg cggtggcgcc
     1261 gatgcagttc gtcgagatca cgccgcccga cgggttgacc ggaatcttgc cgcccatatg
     1321 ggtgttgccg tcccgcacga agcgtacgcc ctcgccgggg ccgcagaacc cgatgtcttc
     1381 gatccacttg agccccgccc aggtataggg caggtaaagc tcggccacgt cgatctcctc
     1441 ggcgggcttc gtgatccccg cctgctgata ggcatccttc gacgcccgct gcatcgagac
     1501 gagtcccttg ttgtagtccg tggaatcccc gaaccaggtg tacgtatgcc gcaccgccgc
     1561 cgccttgaac caggccggct tccgggcgat cttctgggcc acgccgtccg ccgcgagaac
     1621 gcaggcgcag gcgccgtcgc tccgcgggca gatgtcgccc aacttgatcg gataggccag
     1681 catcggcgaa gccatgatgt ccgccacggt cagcggccgc tgcaggtgcg cgaaggggtt
     1741 gttgagcgcg ttgttccggt cccgttcgcc gatgatcgcg gcatcctcct gcgtggcccc
     1801 gtacttctcc atgtaggccg tcgcctccgt cgccaggctc ggaatcgccc cggaatacga
     1861 aaaccggtcc cagatggcgt ccgaacaggt gatgatggcg cccgtcgtgt cgctctccga
     1921 gtttttctcg aaaccgatcg ccagcacgcg gtcgaagagc cccgaggcca cgtggtaata
     1981 ggcggcgatg gcgaccgtcg cccccgtcgt cccgcccgtc gataccttca tgatgggctt
     2041 catgacgccg cccgatcctt cgacgctgta tgtgtcgacg taattgatgg attcgaagtg
     2101 gtccatgttg ccgatcacga tggcgtcgat gtcccggatg gtgagttcgg cgtcggccag
     2161 ggccgccgta acggcctcac ggatcatctc gcgcgaattc acgtccggcc ggtggctctt
     2221 gtgataggtc tgaccaactc cgacaatggc tactcttctt cccatatcgc acctcacttc
     2281 cttccgccca cgatccacac gcagtggctc tggccgcagg gtccgttgaa accgtgtgca
     2341 agcgccgttt cggcgttctt cacctgccgg gccccggcct ccccgcggac ctggagggtc
     2401 gcttcgatca gcctcgcgag ccccgccacg aggatgggat tcgccgaaag cgccccgccg
     2461 gagggattga cgggaagcct gccgcccatc ggggtgatcc ccttctcgat cagggaggca
     2521 ccctcgccgg gtttgcagaa tcccatccct tcgagccaca ggggttccat atagctgaac
     2581 ggctccagga cctccgccac gtcgatctcc gccatgggat cggtgatgcc ggccatcgca
     2641 taggcgcgcc gagcggcgtc cttgagcgcc acgggatcgg cgagccggcg gtcgcccagc
     2701 agatactcct cgcagcaatg cgcgaccccc ttgacccaga caggtttctt tcgggtccta
     2761 gccgcggttt cctcgttcgc gatgatgatc gcggccgccc cgtcgctgat ggggcagcag
     2821 tcgaggagct tcagcggctc ggcgatcttc cgcgacttca tgacgtccgc caccgtgatg
     2881 tccatcggca gctgggcgta ggggttgttc ttcgcgttgc cgtggttctt cacggagacc
     2941 tgcgccacat cctcctcgcg gatgccgaaa cgggtcatgt acgtattggc ctggagcgcg
     3001 gccgcggaaa tggcgtccat ccccaggtgg cggtgaaaga cggggtcgaa catcccgttg
     3061 gtggtgatgg gaacgctgcc ttcgctcccc ttgtaatggg ccacgacgat cgtggccttg
     3121 tacccggaca gcacgcgcat gaggccgtaa aaggcgccga acgcgccgtc ccccgagacg
     3181 gtggagatgt tcttgtcctc cccgccggcc gcgtcgccga ccgccatgcc ggagatggtc
     3241 cggccgtcga agaaatcgtt cgacacggtc accacgctgt cgatgtcgcc gatcgcaagc
     3301 cccgcgtcgt ccagggccgc ccgggtcacg tcgaagacca tatcagccag gatctggtcc
     3361 ttcttcctgc gttcatattt cgtctgcgcc acgccgacga ttgctgcatt gtccattctc
     3421 attcctccac cggcgcttaa atgagttcga aggagtcaat atccaggatg gacccggtgc
     3481 gctcctcctt gaatttcgcc ttcaccctga ggccgttttg gatcttgccc agatccttcg
     3541 tgatcaggtg caggaatccc acgtccgccc catccagttt gatcagtgcg taagcgaacg
     3601 gcggtttcac cggctggacg gggtacgcgt aatgcacgat cgtgtgggcc tcgatgaccc
     3661 cctccgcggc aacgtccacc cactcctcga tgtcgacgaa gcagcggccg caactcctgc
     3721 gcggcggcac gaagaccgtc ccacaggacg ggcatttcgt ccccagaatc ttccgatcgt
     3781 ccctcaggga catcagaaac cggctccccg tgtcgcccac ccaccagcta taggggacgg
     3841 cgatctggcc cttcagggtc aggggttcca cgtctttcaa ccattcagcc atacctttac
     3901 ctctctcatg tgatgatgca tctcatgttt ccggtgaggc gtccgccgcc ggccggcgcc
     3961 accggatgat gcgggaatcc tacgcatgcg ccttcaggat ctcctccgcg cagatcatgg
     4021 cggaattggc ggcggcattc attccgacgc cacgacccgt atagcagtcg cccgcaaagt
     4081 acagcccggg aacctcgggc aggtgaaccg gcggccggta tcggcccgtc atgcccgggg
     4141 agcggccgag gccgtcgatg cccacgtaga agccgtcctg ccgccacagg agatcccgtt
     4201 ccacgttggg gaacatccgt ttcacaagtt cccagaaggc cgccagggtc ttctcccgga
     4261 aggccttgtc cgacgcctgt gccggcgtgc agcaggcccc gatgaaggcg atcatgtgat
     4321 tcgcgggcga gatcgtgtcg tcgaaaagcg tatgcccgag aatctgcagg ggcaggccca
     4381 cgttcggaag ccgccaggcg gacaggtagt acggcgtcgt gaagaggggc gccttcgtac
     4441 caatggtgaa ccccatcgac gcgctcgtct cccgctcgag ggtcggcatg cgttcggccc
     4501 atgccggcgc atacctcgcc atctcgtcca ggaaaagaat tttcagcatg ttccagatgg
     4561 gcatcgctat gacggcgatg ggggcccggt aggtctcggc gtccgtcacg atccccttcg
     4621 gcttccccgg ctgcacatta acgcggcgga ccgacgtgcc cgtcagaagc gtcccgcccc
     4681 gttccgtgta cgccgcctcc atcgcctgca tcatcgtctc gatgccgccc agcggcatgc
     4741 gcacctgggc cagggattcg ccttccttta cgttgaggcg cagcgcatct ccatagatgg
     4801 ccacgacctc cccggcggaa atgtggtccg cgtccgtcag cgtcgtctgg cacatcgcga
     4861 tggttcttag aaactccgca atccggggcg acgcgatttt ccaccggatc agttcgttga
     4921 gcgaaacccg gtcgagggtg tcgagctccg tctcgtccac cgcccgcatc caggtttcga
     4981 gatcggccag ttccctgtcg tccgatgtcg tcaggttcag gtattccggc aggagcttcc
     5041 actgcccctc gtcgtagagc atggcccctt cgatgttgcg cgaccagggg atctctttcc
     5101 ccaatctctt cagaagcgtt gcgcaggccg aatacttgcc gagatcgacg gcatgcgtcc
     5161 cggaatccac cttccacccg ccgggcaggt ggaaggtctt gcagcgtccg ccgggcgtct
     5221 tgctcttctc caggacgagc gtcttcagcc ctttcccctg aagcagggcg ccgacgccga
     5281 gcccggcgat gcccgccccg aggatgatcg cgtcgtaccg gtccatcctt ctcctccgct
     5341 cctccgtggc cgctccgccc ggtccggtgc cagatcgcct gcaggaaacg ggcgatcgtt
     5401 ttccgttcca tgagcggggg cggcgccttt tcggacgccg cccccgcctt cttccgtcac
     5461 accttgatga ggatcgcctc gccctgcgaa agaccgccgc agatggccgc gacgcccgtt
     5521 ccgccgcccc ggcgcatcag ttcgtacatc atcgtcatgg tgacccgcgc gccgctcgcc
     5581 cccacgggat gtccgatggc gactgcccca ccgttgacgt tcgtcttgtc ctgcagggcc
     5641 tgccacttcg ccgggtcgtc ctttgccagc atcttgagcg acaccagcgt caccgcggcg
     5701 aaggcctcgt tgatctcgat cacgtccacg tcgtcgatgg tgtgtccggt cttggcgaac
     5761 atcttctcga tcgcctgggc cggcacgcag gccatgtatt tcggggaacc cgccgcgcac
     5821 gcgcaggcct cgatcgtcgc gagcggcttg aggcccaggg catcggcctt tttccgcgac
     5881 atgatcacca tcgccgacgc gcccgaattc atgcccgggg cgttccccgc ggtcaccgtc
     5941 gggctgccgt agacggtctt cagggccgcc atcttctcca ggctcaggtt ctcccggggc
     6001 gcctcgtcct tctccaggac gatcggcgcc ttcttctgag ggatctcgat gcgcatcagt
     6061 tccccgccca cctcatattt gccggctttc gccgccgcaa aatacttcgt gtgactggcc
     6121 agcgcccact cgtcctgctt ctcgcgggtg accccgtact ccagcgccac atgaccggcg
     6181 tccgtcgcca ccggggcgaa ccccttacgg ctgtagccca gctcaaaaag cacatcctcc
     6241 agcgcgacgt gccccaaacg gctgccccag cgcgtcttct ccgcggaggc gatcagcggc
     6301 acgttcccca tgttctccgc cccgcaggcg acgacgatct ccgcatcccc ggacttgatg
     6361 gcccggcagc ccagccgcag cgccgtcatg gacgaacagc aggcccggtc gatcgtgagc
     6421 gagatgctct cgtcgggaaa ccctgccaac agcgtgatct gccgggcagg gacgttcgtg
     6481 tagatggcgt attccgccgg gatgcaggtg ccgtaataga cctcgtcgac ctgcttggga
     6541 tcgagatcga tccggcggac aacctccttc agggcaagca cccccaggtc catgctcaaa
     6601 accgttttca agaccccgtc gaaacgcccg aagggcgtcc ggacggcgct gaccaccacc
     6661 acatcatttg cattcatttt catgacctct ggtccctttt cccgggatct tccgataccc
     6721 gttcttttcg tgaaaaccac cgccgtccgg gttccgtccc gatctcccac ggcccccccg
     6781 cggacagcgt ccggctgttt tacctcgttt aatggaatcc gaaatccttc cccaggatct
     6841 cgcccgcgca gatcatgccg gacacgatgg cgcaatccat ggcgacaccg tacccccggg
     6901 ccgtatcgcc cgcaaaatag agcccccgga catgctccga cttgaccggc actttctgcg
     6961 ttccggcctg ggagatgctc ttcgccacgc cttcgagctt ccagcaaacg acatcgagct
     7021 tccagtccat gctcgccttg aatccgggat agacctcctc catgaattcg ggcagccggt
     7081 tgacgatctt cttgaccagt tccggcgtcg tggcctcttt ctccgtcaac ggcagataac
     7141 cggtgtagag gtacttgccc gtcggcgccg aggtcggatc cagggcggac tggatgttcc
     7201 agcagatgta gacgtcgaag tcgaatcctt cggatttggg caggacgcag gtgttcttga
     7261 ggcccatctt cgcctcgtct tccggcatca cgagcttgtt caggccgatg tacgggcagt
     7321 agctcccgta gccgtacatg cgctcgatct tttccggata atcggccggg aagtgggcct
     7381 tgtcgatgac cttgaaggtc tgctgcgccg ggatgttgct gacgaccttg ccggtcgtga
     7441 aggtctgctc tccttccgcg gtgtcaacca gcacaccctt gacggcgccg ttctcgatga
     7501 ggaccttctt caccttcgca ttgtagagga tgtccccgcc caggccccga tatttctcga
     7561 cgaccgcctt ggaccattcc atgacgccgt tttcgacgaa tccgccgacg tagcccagca
     7621 cgccgcgcat gagtttggga ccgaaggcgg tcttgaagta ccggaagatc tcgccgatgg
     7681 agatgtcctc cggactgttg atggtcgaaa agagggtcgc caccgagtgg aggtggttga
     7741 aaatcacgtc gctcttgctg atcccctttt cgtccgccca ctgtttgaag gaaacctttt
     7801 cgagcgcgtc gagctgctcg tccgtcatca tgtgggcatc cgcatagaag gagaaaaacg
     7861 ggatcttgtt ctccttggcg atgcgcttga gttcggggtc gatcttcgag ccggccacgt
     7921 cgtgataaag cttgcctttg taataatttc cccacatgcc gccgtgcttc ttgatctccg
     7981 gcaaaccgcc gatctggtcc tcgaagtcga gcatatagcc ggccccgttt gcgctcaggg
     8041 catgatagcc gatatccagg gtgtagccgt ccagcatccg gttcttgatg atcgtctcaa
     8101 tgtcgggctg tgaggaggcc aggtaggaat actgggcggc cagcaccttc ttgtaccagt
     8161 cgaacccgtt gtcgcagatc tccttgccct tgatggacag ggcgcgtccg ccgggacgat
     8221 cgcactgctc caggatcagg accctcaggc cttccttcgc catcagggtt ccaaccgtca
     8281 ggcctccgac tccggcgccc acaacaatcg cgtcatactg tttttccttc atgtccaacc
     8341 ctttctttga gtgtattttg agcatcacgc tctatagata catcagttca tgaagcatct
     8401 gaccggaaac gacgtcgcgc cggccgtcga cctccacgtc catcgtgtag ttcgccagga
     8461 agcggtagta gtactggttc caatccgcaa tcttgggcag ctgcatggtt tcgatcatcc
     8521 gcgtcgtgtc gagggtgagc gtcattttga ccccttcggt gtccgtcgtg atgacgagct
     8581 tcctggcgta gtcccggccg gtcttctcgt cgcggaccat ctcctccagt tttgtctcca
     8641 tccggtccgt ggacaggacg atctctccgg ggcgcgcaat cagaagcggc gaaaccaccg
     8701 gtgcctcctg ggcgcgcggg atgacccacc cgtagtcgat gatgtatttg tcgttgtgga
     8761 tgcgccccca gtaccagccc gcgaagatat cgaactgggc cttgttgccc cagttgtggt
     8821 catgatagca ggcgccccgg acggggatcg tctcctcctt gagataaagc tcgcccgtga
     8881 cctccgcata gggaacggcg acgacccagc cgccgatcgc cccctgcgct tcgtcgtgat
     8941 aaaggagccc cgtgccgggc ttccagccgg ggacgacggg cttcagcgaa aggcgggcgc
     9001 cgacgccctt gatcttgatg tacatctcat aggtggcccc ggcgtccttg agccacgcct
     9061 cccccatccg gacatcgcag tagtccgggc aggcgtagat gtctttctcg tcacacaggt
     9121 cgtagcgggc cgtctgcgtg ccgtccggct tgtacaccat gagctggatg gacggaacga
     9181 tcggccttag gaaggcgttc atgtaatgga aggttacgac gagatggtag ccgttgtcga
     9241 acgaggcatc gacataccac cactcgtagt aatccgtgct cttgtagatg tggttgccgt
     9301 cgtcacgccg ctggtaattc gacggatttc gctcggcgcc ccgccgaccg ttccacatgg
     9361 acagataatt cttcttctcc atctcgttca tgtccatccc tcagagaggt ttgaaatact
     9421 ggatgtccag gatgttcgcc ttgcggtccc gggcgaaaac cggcctcacc cgcatgccga
     9481 tatggacctc gtcgagatcg acctcgtcga ggaagtgggc catgcccgta tcggcgccgt
     9541 cgagcttgat cacggcatag acgaaggggg ccttccgggg ctggacgggc tcgttgtagg
     9601 tcacgaccgt aaaggtctcc accgttccca gtggaccgat ttcgacggtc tccgtcaatt
     9661 ccgaaaaaca gacttgacag gtcgagcggg gcggccagag gaccttgttg catttagggc
     9721 atttcgcccc cagaattttc tgctggtccc tgagcgcgac ataaaagcgg ctgcccatcc
     9781 ggccggcata gtagcggctg ttggccgtca ggtgctgatc gacctcgaag ggttcgtagt
     9841 gtttgatctc ttccatcgga ttcgctcctt agctttttga gatgaaattg ctcgccagcc
     9901 tcacttcggg cggacctggc cgggcagcgg ctcataatag aggatgtcgc tcaggtcgcc
     9961 ggtccgctgc tccttcgggc gcaggacgag gcgcatccgc atccccaccc agaccttctc
    10021 catatccggc tccacgttga cgaaatggtc gagtaccgac gtcgcgccat caaggcggac
    10081 ggacccgtaa acgaagggcg gctcgtgggg tttccccgtc gtgggatccg tcagggggag
    10141 atacatgatc gtgaaggcca cgagcgtccc ctcctgcgcg agttccaccc actcctcgtt
    10201 tttgacatga cactcggcgc agacgatccg gggcggcaac tgcacccgct tgcaccgggg
    10261 gcatttcacc gcgaagatct tgccgttatc ccgcatttcg accaggtatt tcgtcaggta
    10321 ctgaccggcg gcaaaccggt aggggacgtc caccgtcatg gggatcgaga tccattcctg
    10381 ttctttctgt accatcttca cgcctcctca atcatccagg gtcttcgaca gggccgtcat
    10441 gaccgtccag ttcgtgccgc cgtatcccgt cgtcagggca cggcggacgt ccttcggcac
    10501 ctgatgtccg ccggcgtccc cgcggatctg cagggccgcc tcccagactc ggagcatcgc
    10561 cgacgccccg atgcagttcg tggacagaac ccccccggac ggattcaccg gaatggggcc
    10621 gtcgatgttc gtcatcccct tctcgacgag gcggaatccc tcaccggggc cgcacagacc
    10681 gagatcctcg taccagatca gttccgccca ggtcgcgggt tcgtagacct cgaagacgtc
    10741 gaaatactcc gccggtttct tgacgcccaa tttcttgtac atgcgcgcgg cgcacacctt
    10801 ggagaaaatc ggccgcttcg tgtcgacaaa ccgccagtcc aactcgaact gctcgttgtg
    10861 ggccgtatcc atcgcccgga tccagacggg cttcttgcag cgcttcttcg ccgtcttttc
    10921 gtcggccagc accatcgcgc aggcgccgtt cgaggcgggg cacatgtcga gcatccggat
    10981 cggataggat agcatccgcg aattgagggc gtcctcgatg gtgatgtcca gcttcaaatg
    11041 ggcgtagggg ttcttccggg cgttctgacg cgccttgacc gcgaccatgg cggaatgctc
    11101 ctccgtggcg ccccaggtgg ccatgtagga cgagcccatc gtggcgaaat agcccacggc
    11161 ggcgcccgcc agggaacggc cccagagggg atgggccacg ccgctcagga tcgctcccgt
    11221 atcgccctcc tcctgttttt cgaatccgat gcacagggcc ttgtcgaaga ggccgctggc
    11281 gatgtggtgg gcgccgacgc agaccgtggt cgcgccgacc gtgccgccgg tcgtgatctt
    11341 gagccccggc ttgccgtagg ccccggtacc gtggacgagc cagcagtcgg tcatgtggac
    11401 cccctcaaag aactccatat tgcccaggaa gaccgtatcg atctcgtcga tcgtcatccc
    11461 ggcatcgtcc agggcccggc ggaccgcttc gttcaccatc tccacctgat ctacatcgtg
    11521 gcgccgcgcc gcatgatagg tctgtcctcc gccgacaatg gcaacatttc tcttcatgat
    11581 cgatcctcct actcctttcc gaggacaacg acgcactggt gctgtccgca gaaaccgctg
    11641 tgtccctggg ctacggcgac cttcacgtcc ttgctcacct gatggtcccc cgcctggccc
    11701 atgacctgca gggccgcctc cgccacacgg ttcaggccgt acacattgat gggcacgccg
    11761 cccaggaggc cgccggaggg gttcaccggg agctttccct tgatctccgt ggcgcccgac
    11821 tccaccatcc gccccgccgc cccccgatcg cacaggccga gcccttccat ccagaggagt
    11881 tcctggtagg aaaagtcgtc tccgacctcc gccacgtcga tctggcggcg cggattcttg
    11941 atcccggcca tccggtaagc ctgggcagcc gccttctcca gggcgaacac accggccaga
    12001 tcgcggtcgc ccaggtagta ggcgtcgtag caggtcccga tccccttgat ccagacgggg
    12061 ttcttcttga tctggcgcgc ccgcttctcc gaggccagga tcatggccac ggcgccgtcc
    12121 gtgtccgggg cgatgtccag tttccggatg ggcgaggcca cgagcggact gctcaaaaca
    12181 tcggccaccg tgaaatcgcc gctctgatgg gctacggggt tcttcttcgc gttggcgagg
    12241 ttcttcacca ccacccgggc gatctgttcc ggggtgacgc cgtacttgta catgtagcgc
    12301 cgggcctgaa gggccgccgc cgaagtgtag tcgaacccca aaaggcgcgt gtagatggga
    12361 tcgaacgcgt cgttgttcac gatattccgg ttcgtctggg acatcttcgt atgcgccatc
    12421 aacagggtgc actcaaattc cccggacagg atgccgatgg tcccgtaata gacggccgtc
    12481 gagccgtcca gagccatctt ctcctcgttg cgcagatgcc cgccgatcac gtcggtgatg
    12541 ccgatgtcgg aaatggtctg gccgtcccag atgtcatggg aacaactgat gctgttatcg
    12601 atatccttgt ccatcttgag ccccgtctgc cgcaggaccg cctcgatgac ctcgtaggcc
    12661 atctcgccgt aactgacatc cgcgcgcttg ggggcaaact tcgtctgggc gatgcccacg
    12721 attgctaccc gatctgccat gaaatcctcc ttgcccgtgc cgtcgatcct cgaaaccacc
    12781 cgtctccggc gaagccgctg cccgtccggt ggcgggtccc ggcggaagtc gttttccgcc
    12841 ggggacaacc gacgccagat gcgttaaatc cgttcaaaga tcgtggcgat gccctgaccg
    12901 ccgccgatac acagggtggc cagcccgtaa cgcccgcccg tccgctgcag gtgcttcagc
    12961 accgccaggg ccagccggcc gccgctcatt ccgttgggat gaccgaaggc tatggcgcca
    13021 ccgtaggggt tccatttctc cggatcgacg acgtggccgt tcttcttaag ttccttcatc
    13081 acggccagcg tctgggaagc aaaggcctcg ttgcactcca cgacatccat gtccgccagt
    13141 ttcaaaccgg cccgctccag gaccttggga acggcgtagg ccggtgcaat ccccatatat
    13201 ttcggttcca cgccggccag ggcacaggtg acgaagcgtc ccaggggctt gagtcccatg
    13261 gattcggcct tgtccttgga cataacaagc acggccacgc cgccgtcgtt catccccgag
    13321 gaactcccgg ccgtcaccgt gcctcccttc ttgaaggcgg gcctgagggc ggccagtttc
    13381 tcaaggttgg tcgcccgggg gtgttcgtcc cggtcgaaga cgatgggatc gcccttccgc
    13441 tgcggaatcg tgatggggac gatctcatcc ttaaagtaac cggcctcgat ggccttgatg
    13501 gcgagcgtct ggctcctcag accgaaggcg tcctgctcct cgcgggagat cccccactcc
    13561 tcggccagaa tctcggcaac gatccccatc ggggcgttcg tctccggcgt cgggccgacc
    13621 tccttgccga tgaaggcgtt gggcatcagc gaaaagggaa ccttctgccg ctccatcagg
    13681 tacggggcat tgctccagct ctcgcagcca ccggcgatat agacgtcgcc gaagccggcc
    13741 agaatcgcca tcgccgcgga attgatggtc tgcagtgcgg agccgcactg ccgctccacc
    13801 gtctgggccg tcacggatac gggaaagccc gccttgagca ccgcccagcg cgcgatgttg
    13861 accgcggcgt tgttcgtgca ggcgtggccg atcaggacgt cttccacgat ggccgggtcg
    13921 atcttcgtct tttcgagcag tcccttcagg gcaaaggcgg ccagctcctc gggacggaac
    13981 ccggacagcg cgccgcccat ccttccgatc ggtgttcgga caccgtcgac aatccacact
    14041 tctttcatca tttcacctca atctcttccc gcgccgcgcc gggagcgcga agcggtgcgt
    14101 cttctcatcc aaatcctcca cggaccgggt cgccccctcg ttgaggggtc cggcccgcag
    14161 cggaacagcc tcacttgccc ttgaactccg gcttgcgctt ctccaggaag gcgttcatcc
    14221 cctccacccg gtcctgcgag ccgaagcaca tgcagaagca gcgagcctcg atggacagcc
    14281 ccgtgcgcag atccgcctgg acgcccatgt tgatggcttc cttccccatg gccagggcga
    14341 tggggctctt ggaggccagg cgcttgcaga gtttcgccac ggcgccgtcc aattccgcct
    14401 tggggacaag cacgtccacg agaccgatcc gataggcctc ttgggcactg atgtgctcgc
    14461 cggtcaggac cattttcttg gccatcccca ccccgacaag gcggggcagc cgctgcgttc
    14521 cgccgtagcc gggaatgatc ccgagattga tctccggcac gcccatcatg atcgtatccg
    14581 ccgccacgcg gatgtcgcag gcgagcgcga gctccgtccc gccgcccagg gccaggccgt
    14641 tgagcctggc cacgaccggc ttctgaaaat gctcgatgtt ccaggtgatc tgctgtccgg
    14701 cgggaagtcc gtggatgaca tccagatacc cggcgccttt ggccatgatg gagatgtccc
    14761 cgccggccac gaaggccttg tcgcccgcgc ccgtgagcac caccacgcgg atggcgtcgt
    14821 cctgggcgat gccgttcagg gcgtcctgaa tctcgtacat cgtgttgtcg cgaatcgcgt
    14881 tcaacacctc cgggcggtcg atcgtgatcg tcgccacggc tccgtccttc gtcaacttga
    14941 tgtctttata gtccatgcaa ttcttctcct tgtcatgaaa atcgttcact ccgcagcgtt
    15001 aaccgaagat cttgaagggt gtcttggtcc cgtcggggtg gtaatcgtag aaccccttgc
    15061 cgttcttccg tcccaggcgg cccgcctcga gcatcttccg gtagaggatg ttgagcttgg
    15121 cggactcgct gtgggtatat tcgaacacgg cgtcctgggc catctggatc acgtcgaaac
    15181 cgccgaaatc cgccagttcg cagggtccca tcggatggcc ggccccgagc ttcatggccg
    15241 tgtcgatgtc ttccgccgtc gccgtcccct ccaggaccat ctgggccgcg tccatgtaaa
    15301 gcggacccag gaggcggttg acgatgaacc cgggggtgtc cttcgtcctc acgggaacct
    15361 tgcccatctt cttgcagacg gccatggccg tctcgacggt ctccgcggcc acgccgatcg
    15421 gcatgacgat ctcaaccagt ttcatgacgg cggccggatt gaagaaatgg gtccccacga
    15481 ccttgtcctt gcgttccgtc gcgccggcaa tggccgtgac cggcaggacc gaggtgttcg
    15541 aggcgagaat cgccgccggc ttgcagatct tgtcgagttc ggcaaagatc ttcttcttga
    15601 ggtccagact ctccggaacg gcctcaatga cgaaatcggc gtccttggcc gcgtccttca
    15661 gatccgtcga ggtcctcagg agcttcagcg tcgcgtcggc ctgctcctgc gccagctttc
    15721 ccttctccac gcgcttaccc aggctcttgc ggatctgggc cgcgcccttg tccgcgaatt
    15781 cctgggccac atcgtacagc accacgttga tgccggcgga gatcgccgcc tgcgcgatgc
    15841 cgtgccccat cgttccagca cccaatacgg tcatgttctt gacgtccact tgttgaatcc
    15901 tcccttcgtt catcgtgata ttgcgggttc cgttatcgtc acttctttgg aaattccttt
    15961 tccatctcct ggtacagatc gagtttcttc accttgccca gcagggtcag gggaagctgg
    16021 tcgcggaaga cgatatcctc cgggatcttg aaattcgcca gatgatcctt gagaaacgcc
    16081 ttgacgcctt ccgccgtcgg gttggtcccc ggcttcttca tgatgtaggc ccggccgacc
    16141 tcccccatga cgggatcggg ccttcccacc acggccgaca tcagaacatc ggggtgcttg
    16201 gccagggcct cctccacctc ggggggatag acgttctcgc cgcccctgat gaacatctcc
    16261 tttttccggc ccacgatgta gagatagccg tcctcgtcga gccgacccat gtcccccgtg
    16321 tggaagtagc ccttgggatc gaagacccgg gcgtcctcat cgggccgttt ccagtagccc
    16381 ttcatgacgg ggtctccctt gatggtgatc tcgccgacct cccccgtggg caggatattg
    16441 aacttgtcgt ccatgatggc aatctcgacg gtattgatgg gcgccccgac gctgtgcttg
    16501 agcttttcct cgccatgact ggggtgcgtg aaggtgatgg cgccgctgac ctccgtcagg
    16561 ccgtaggcgt tctgcggcat gacccccatc cgctgcttga tcgccagaat cagctccgag
    16621 ggacagggct ggctggagac gatggccgtc tgaatcgtgc tcagatcgta cttctccacg
    16681 ttggggttga gcagctccag ggcgtattgg gccggcacct gcccgatgat ggtcacgcgt
    16741 tccttttcga tgatctgcag ggtctcctcc gggacgaaca tgtccatgat gacgagcttg
    16801 ttccccgagt tgagcgtggc gatgaccgcc atgacggcgc cgcccacgtg gttcagcgga
    16861 atgttcaaca gcagcgtgtg ccccttcccg taatcggagg ccgcattctg gccgttcgta
    16921 taggagatca ggtttttatg ggtcagcatg gccgccttgg gaatcccggt ggtgcccgac
    16981 gtgtagagaa acagcatggg gtcatccggc tcgacggccg cctgggcctg ctccagggcc
    17041 gggcccggtt cggccagaaa tctcgcccac gggatcatcc ccgcctcggc cttgtcctct
    17101 tcgaggatca ccaccttctt cagcagcggc agttccttcc gcgccgcctt gaggttctgg
    17161 acgaagtcca tccccatgaa gccggccgcc atcaccaggg cggagattcc ggcgtcgtcc
    17221 aggacgaact tgatctcctg ggccgtgaag cgccagctga ccgggacggc cacggcgccg
    17281 atcttggccg cgcccagata ggcgaagata aattccggcc agttggggac gtagatcccg
    17341 acgcggtccc cccgcttgac gccgatgccg agaagcccca tggcgacccg gtcggtgatc
    17401 tcgtcgtact gccggtacgt gaagcgccgg tccttgtaga cgagcgcctc ctggtcgggc
    17461 tgttccgctg cgtgctgcct gaagaaatca tccatcactc gcgccatgac agacctcctt
    17521 tttttatgcc ctgcggacga tgtatcgttg acatctctcc tcccgaccgg cgcccccgga
    17581 acgtccgggt caacgttccg gaggccgagc cgaaagcctt ccacgcctgt gccgacctgc
    17641 ggctcccctc gcgccgccgg tttcgaaacc gggcatcgcc ggggacaggt cctgcgaaca
    17701 gcatcgaatc ctgttttcaa agaacgcggg accggccccg cgcggcaggc gcgaaacgcc
    17761 tgccgccgcg ggggccttct tccctaagcc acggacgaaa cgtccggcgg cgaactactc
    17821 ctgcggacgc tcatccacga tgcgcgtcgc cttcagtgta gcgccggggt tgaagatctc
    17881 ggagagcggc acgaactcca ccttcggcgt caattcgacg gccttcttca ccttctcctt
    17941 caggaccttc tccagcgagg ccagatcggc ccctttatcg gcgccaaccc gaagtttcag
    18001 catatccatc gaatagggat catccgcctt ctccttcgta acgacgaact ggtactcgac
    18061 cacgccgggg gtattggcca cggcatcccg catcgtttcg gggttgatca gggtcccctt
    18121 caccttcatc aactccttgg tcctcgttcc gtaggtgctc cccacggcgg tcacgatccg
    18181 ctggtcgttc cggccgcagt gcggacaggg cttgttggtg acggcaacga tgtcccccac
    18241 cagatagcgc agcagacagg ttccccggcg atccaggtgc gtaatgcaca gcaggcccgg
    18301 ctcgccgtcc ggcttgcgtt tcccgttctc atcgacggcc tcgaaatagg cgagctgcgg
    18361 ggttccggaa tgggcacccc ccagttcctg acattcctgg aaggcggtct gcgcctcggt
    18421 aaatccgaac gcgttgctga tatacacatc gcccgcgccc atcccctgaa ggcgccggcg
    18481 gatatcgtcc cgcatcccct tgggaacagc ctcgcccagg gcctggatct tgttgatccg
    18541 ggagaggtca cgcccctgct cctcggccac catgatcaag cggcggatgt aggaggcgat
    18601 tccggagagg gcattgacct tcttcttctc gatgtcctcg atcgcctgtt gcatggagcg
    18661 gtggatgttg taggggaagc ctggcagcgg cgcgccggtg aagccccagc acatcgacat
    18721 gcccacggcg gaggcgaaat ggacggtctt gtagtacccg atatggggaa tgaagtgata
    18781 ggggaagagg ttcatcagga tgtcgtctgg ctcgagatag cagagcttgc aaccgatccg
    18841 gaacatccag gactgcatga acatgtcata ggttgtgttg tagaaccggg agggcatgcc
    18901 cgtcgtcgtg ccggtggtgt aggtgatctc gtagaggatg tactccatga tgtgctcgaa
    18961 atccatgtcg agcttgaagg actcgccccg gtccttcatg aagtcggcct tgctcgtcag
    19021 cggcaccttc tccaggtcgt cgagggtctt gatggacggg taatcgaggc cccactcctt
    19081 gaacttctcc cgatagtacg ggctgaactt tcccaggcgc tccatctgct tgacgagcgc
    19141 cgcgtcctgg acgccgcgga tctcttcctg gctcttgaag gacaggtcca gcggctgatc
    19201 ctcccgatac atcgggatgc tgttaatcca ggtcatgacc tcttcggggt tcttcgggat
    19261 cttgaacggt ttgtgcgaca tctgattcct ccttataatg aatttgagat tgactgatca
    19321 atcacactcc tgacgaaaaa tgacccggat accacttccg gggattcgca aaaccggaca
    19381 cggatcgggg aggagtaagc acatcggaac gggagggtcc ccgataacaa aacgatgccg
    19441 acccttttca gtcagcgcga tggcgcaccg cctggcgggc gcgtttccta caacagcgat
    19501 ggggtggccg cccgcgtttc gcaggagggc gcgtccggac ccgaaggccg ggcctgaacg
    19561 ccccgtccgt tcaccaaatt caacgccttc aggcgtcccg agcggatctc ttcacacagc
    19621 tcggccacgc ctttcaccgg ccgttcaaag acggtccggc agatccccac ggtgtgcagg
    19681 gcatgggaat cgctggcgcc ggtcccggca atgcccatgg acgccatggc ctccgccgcc
    19741 tgggttcgag cctcgtcgtc gtaatccggg tgctccacct ccaggccgtc cacgtggaaa
    19801 tcgtagacct gtttccccgc accgtaatac ccctcgtcgc cgtggcgaaa cttcttgaag
    19861 gggtgggcgg cgacgaccac gccaccctcc gcgtggacga gttcgatcac ctcctccgcc
    19921 gaccggctgg agccgaggct cttcggatag ccgaacacga gaaactcccc ctggaagcgg
    19981 ccgtgacgcc agtagcaggc cacctcctga ccgggaatga tcaaaagatc ccggacgccg
    20041 ctcgcgcggc gaagctgttc gatctggtcg tcgctccagc ggatgccgtg ctccgtcagg
    20101 gcaatcccgt ccagacccac ggcccgtgcc ttcttcagga gtttcgccgg atcgatattg
    20161 ctgcagccgg agtaccggtt cgtgtgaatg tggagatcga tgtccatcga cggcttagaa
    20221 tcccatctgc ttgctgatga cttcgttcat gatctcgtac gtgccgccgc cgatggagaa
    20281 gagccgcgaa tcgcggtaga gccgctccac cagatactcc cggcaatagc cgtaaccgcc
    20341 gtggatctgc acggcgtcgt agacgatccg gtcgcagacc ttcgtggcga aattcttggc
    20401 catgcagaca tcccggtagg cgttcatccc ctcgccgatc atggccgcca cgcggtagtt
    20461 gaactgcttg gaggcctcca gcagcgtcgc catttcggcg agtttgtggc gcgtcacctg
    20521 gaaccccgtc aggggcttgc cgaaggccgt gcgctccttg gcatagcgga tggattcctc
    20581 cagggccatt tccgccaccg cgtgggcctc gaccgccaga tagaggcgct ccttctggaa
    20641 gttgagcatg atcccgtaga agcccttgtt ctcctcaccg agcagattct ccgccggaac
    20701 gacacagtcg tcgaaggaga gctccgccgt atcggaggcc caccagccca tcttctccag
    20761 cttcttggaa accgtgaatc cgggggttcc ccgctcgatg acgagcaggc tgatgccgct
    20821 gtgccccgag ccgccggtgc ggacggccgt ggtgacatag tccgcccggg tgccgctcgt
    20881 gatgaaggtc ttcgacccgt tgacgatgaa cgtgtcgccc ttgcggacgg cccgggtcag
    20941 caggttggcc acatcggagc cgccgctggg ttcggtcacg ccgaggacgg ccacctggtc
    21001 gccggccagg acggggggaa cgtattttcg tttctgcgcc tccgtcccca gggccaggat
    21061 cgggggaacc gcaatggcgc tggaacccag gctggagacg agtcccacgg acccgcagcg
    21121 gaggatctcc tccgtgtagg ccacctgatg gaagatgtcc gcgggcgttc cgccatattc
    21181 ctccggcgat cccaggccga ggaagcccgc gtcgccggcc tttttgtaga gttcgagggg
    21241 gaacgttcct tcctcctccc aatgctcgat atggggcttg atttcccgct ccacgaattt
    21301 gcggatgctg gcccgcagca tctcgtgcga ctctccaaaa tagtttacgt acacgccctg
    21361 atctctccct tctcgttcgt tcgtttcatg ccgccccggc ctgtagcacg ggcgtataaa
    21421 gatcctcgta acggccatcc gtctgtcctt cccggaagct ccgctcgacg tggctcgtgg
    21481 tccccccgag aatgatgcgt tgatcgtgga aacagagccc cgcgggtcgc aggctgatca
    21541 gggccaggtc atccttggcg atgtccccgg cgtcgtaacc gtcgatcaga atatccggaa
    21601 tatcctccac cggatgttct tcgaaaagcc cgcggataaa atcctcccgg cgttccggcg
    21661 gcggcgttct gagataggta aagccgtccg tggaaagaag caaccgggat tcatccgaaa
    21721 aggataggta ctccacctgg aaaaaccgag gggtccgacc caccatccag aaattgctcc
    21781 gggtgacctg gagagccgtc gcggcgccca ggtcataatg cagaaggagg ctgtcgccgg
    21841 tgtgaagaag caggccccgg catcccgtcg ccgacttcag gatcagaacg ccggtgaagg
    21901 tgcaactgtc cgtaaaggac atctccccca gaatcttctc ggattgacgt tccacctcct
    21961 ctctgagcgc cgcctgctgc gcatcgtcga aggatcgtgg ggcgccgatg ccgtccaatc
    22021 cctccagcag gccggcaaac cggagcatga aggcccgcga ggacgaggaa ttacgctccg
    22081 aactgtcggc gacggcgaag aatcccttgc ggaagtccag gagcatgcag tccccgaccc
    22141 ccatcaggta gtccggccgt ccggacccgg aacgcaaccg cgcacccagg aatccgccac
    22201 accgcacggc ccggcgcccc tgattgagga atatcgatcc cttccccgcc atcctctcac
    22261 tccgcgagat cttcgaagat cccgatcttt ccgtttgcgc tgaagacttt acctttcagg
    22321 gtttgcgccc gacgagggcc cgggtcgtat agatcacgac gtcttctccc cgctggttct
    22381 tgatcgaatt cttggaaacg atgacgcccc gcttgtcgtc cttggcctgg agatccatga
    22441 tctccccttc gcaatggatg gtgtccccga tcttgaccgc tccgacgaag cggacggaat
    22501 ccatcccgta gaaggcgatg aacgacttgg gcagggccac atagggtccc agtcggaaaa
    22561 tcagggcgct gccgacgcac aggctcagca tcccgtgggc gatgcgctcg ccgaagaagg
    22621 tctttttcgc gaattccacg tccgtgtgga gcggatgcca gtcccccgtg aaggcggcga
    22681 acaacgtgat gtcggtctcg gtgatcgtgc gcgccggaga gataaacttt tcgcctacgg
    22741 tgtaatcctc aaaatacgat ttctccatcc tgtgttgcct ccttcaatcc ctatcggttt
    22801 atgcctagag gtaatcctcc ggaatctcaa cagccgtcgt atcttccatc cggatgatcc
    22861 gcgcgcgccc aaaaacctgg ggcaggaaat tgcggcagta gtaacgcacc gtctccacct
    22921 ttcccctgta aaacgcctgg tcggcccccc cgcattgacc ctgctcaagc gtcccgaggg
    22981 cgatcattcc ctgttccagc aagagatggg caatggccac ctccgccgcg cagtccagga
    23041 aacgggtcga cgtcaacggc accagatcga gcttgccctc gctgagatac ttcgcgtagt
    23101 ctagggcgag ccccgccacg gtctgaaggg cctcctccag aatttccaac tccccggaga
    23161 aggccgtctc tgacttgtgg gtcccgatga aggccgtgac ttcatcgatc cagcccttaa
    23221 agaccgcgcc gccgcccatg ctgagcttgc ggccgaccag gtccgccgac tggatgaagt
    23281 tcgtcccatc ccagatagag aagatcttgc aatcccgggt gtactgctcc accgggtact
    23341 ccttcgtgaa gccgtacccg cccaggatct ggatcccctc ccggcccatc tcgaagatcc
    23401 ggtccgaaca gtacgccttg acgagcggcg tgaagagttc gaggcgaccc ttcgcccgct
    23461 tccgctcggc ctcgtcgagg ctgttcttgg ccagatcctc catgaagtac gtcttgtaga
    23521 ccagggcccg catcccctcg gtcagggact tcaaattcat gagcatccga cggacatccg
    23581 cgtgctccac gatccgcacc ctggcgccac ccttgccgaa cggcgtcccc tggacccgct
    23641 ccttggcgta cgccaggacg ttcgcgtaga tgttcgcccc gaaggccacc gattccagtc
    23701 ccacggccat gcgggcgacg ttgaccatct ggaacatgta ggcaagtccc atgcccgcct
    23761 cccccaccag atggccgcgg caccggccgt tctcgccgaa attcatgacg caggtcgccg
    23821 agccgttgag gcccatcttg tgctcgatcc cgatacaggc cacgtcgttg ggttccccca
    23881 ggctcccatc ctcgttcacc cagatcttcg gcacggcgaa cagggaaacc cccttggcgc
    23941 cggccggcgc cccctcgatc cgggcaatga ccaggtggac gatattctcc gtcaggtcgt
    24001 gctccccgga ggtgatgaag atcttggtcc cctggatctt gtagttcgac ccgtcgggaa
    24061 tcgccttcgt cgagaccatg tgggcgtctg aacccaccgc cggttccgtc aggcacatgc
    24121 ccccgcccca ggtcccgtcg tacatcttgg cgcagaacag gtccttgatc gcctgagacc
    24181 cgtagttttc gatcatggcg ccgttcccgg ggcccatccc ggagaaacag ccgaaggcga
    24241 agttggcgct catgaagaat tcctgggcgg cttcggcgat gatcaggggg ataccctgtc
    24301 cgccgtaatc gggcttgagc ggcagggcaa accagctacc ctccttgaag acattccagc
    24361 aatcgtgaaa cgaccgggga acggtaacct tgccctggtc aaaatggcat ccctcgcgat
    24421 ctccgtcctg aaaggtcggc gcgatatcat tggccgcgat cttctgggcc tggtcgagga
    24481 tcatgtcgaa atcctccatc gtaaaggccg aatacggctc gaattcgagc agacgcttaa
    24541 ttcccagatg ttccttcagg acgaacttga cgtcccggtc gtcgcgcttg taaaaattct
    24601 gccccatgaa aacctccctc aaaaatttgg ccccgggtac cggacatcgg gcttcaggaa
    24661 ccccggccct cgcacgttcc gatcctgcac gcgaatcggc tctccgccgg aaaccccggc
    24721 ccggccgatc gttctcttaa gatttttcgc cctccacctt cttggaaaac cctcgccgca
    24781 ggaccatcat gatctgaccc cgcgcttcct cgacggggaa ttcctccttg ttgaattccg
    24841 gattgtagac gtactggcgg atcgtggcat ccaccgcccc gtgcatgatg atggtggcca
    24901 ggatcggcga gacatccgcg gcgaagacac cctgctcctg cccctcgtga aaaatctcca
    24961 tcaggccgcg gaagaacagg ttgtagagct tccagttttc cgggttgtag aatttgtcca
    25021 gtcggggaac atccatgatc atgaccttga ggagatcggg gttttgccgg taactgcgga
    25081 agatgaagtc gatcaccatc cccagttgtt cctccgggcc ggacccctgt tctcgcacct
    25141 gccttacctt ggcgatcagg agattccaac gttcctgaaa aatggacatg aggatgtcgt
    25201 ccttgcttcg gaagtagtgg tacagagagc cgtacgccac cccggcctcg ttcgaaatat
    25261 ccagggtccg gctggagtag taccccttct cggcaaagac cttgatggcg gcgttcagga
    25321 tgatgtcctt cttctgggtg gttgcttcat ccataagcgg gtcccccgtc catgaagtga
    25381 ctgatcagtc aatcgacaca ctcatagtga taaaaccaac gcctgtcaag caataaattc
    25441 cgcccggggc ggatatattt tcaagaaccc gggatcacgg gagaatcaaa cgttttcccc
    25501 cttgcccgtc acgatacgcc gagcctcttc atgaccgcca ggagttccgc cacggtggcc
    25561 gccgactgct tcagcgccgc cgtttcggtt tcggtcaact tgagctcgat gatctgttcg
    25621 atccccccgg cgcccagctt gaccggcacc cccataaaca gaccccgaat cccgtactcg
    25681 ccatccagat acgcggcaca cggaaggatc tttttcctgt ccctcaagat cgcctccgtc
    25741 atttcgacgg cggccgaggc gggggcgtag taggcgctgc ccgttttcag gagctggacg
    25801 atctcggcgc ccccctgccg ggttcgttcg accagcgcgg cgattctctc ctccgggatc
    25861 agttccgtga tgggcacccc ggccacggtc gaataccggg gcagcgggac catcgtgtct
    25921 ccatgtcccc cgagaacgcc ggcctgaacg ttgtccgccg aaactttgag ctccatcgca
    25981 atgaaggccc ggaaccgcgc cgcgtccaga acgccggcca tgcccacgac ccgctcccgt
    26041 ggaaaaccgc tggcctcgag ggcaacgtgg cacatggcgt ccagtggatt gctcacgacg
    26101 atcaggatgg cgtgcggaga atggcggacg gcttcggtca ccacctgccg catgatcttc
    26161 atgttcgtcg cgaggaggtc gtcccggctc atccccggct tccgggcgat gccggccgtc
    26221 acgatgacga tgtccgaacc ggccgaatcc gcgtaacggt tcgtcccgac gatccggcag
    26281 tcgtgacgct cgatgggggc ggcctccgtc aaatcgaggg ccttgccctg gggaaccccc
    26341 tccacgatat caacgaggca gacctcgcac agcgccttct ccgcgagacg ctgtgccgcc
    26401 gtggcgccca cattcccggc accgaccacc gtgacctttt tctgcatgca aatccccctt
    26461 ttccggcttg acacgcgggg acgctcacga aaaacacgag aaagtccacc gcgcgcttcg
    26521 accgtctcct tatctcaatt cgtcgccgaa ctcaagagac tggatgacat gaaacgcgga
    26581 cgcccgtgaa aaggttggac cccttccgcc gagggcggaa acccgaaatc cgacgggcgg
    26641 cgcccccttc attttccggt tgcaattccg cccgggaagg ggtagacagt gacgcttttc
    26701 tgaatgattc cggcggggca agaaccgtaa tgcgttgagg agcacgccac cgtgcggcgg
    26761 cccgatccca gcggcgcaaa aaggcccacc aaggaggagg ggtatgggaa aactgttcgg
    26821 aacggacggc gtgcgggggg tcgccaatca ataccccatg accgcggaaa tggccctgaa
    26881 catcggccgg gccacagccc tgcagttcaa gcgggcaggg catgccccga aaatcattat
    26941 cggcaaggac acccgggttt ccggttacat gcttgagaat gcactcgtat ccgggatttg
    27001 ttccatgggg gtggatgcca tcctggtcgg ggtgctccct acaccgggca tcgcctacct
    27061 gaccaacagc atgcgcgtcg acgccggcat cgtcatctcc gcctcccaca accccttcca
    27121 ggacaacggc atcaagatct tctccggcga gggcttcaag ctccccgacg aaaaggaagc
    27181 ggccatcgaa gacatgatct ttgcgaacaa cctggacaaa ctccatccgt ctccgaccga
    27241 actgggcaaa gcgtaccgga tggacgatgc ccgaggccgc tacatcgtct ttctcaagca
    27301 cacctttccg aaggagtaca ccctggaagg catgaagatc gtcctcgact gcgccaacgg
    27361 ggccgcctat cgcgttgccc cggaaacctt cctggaactg ggggcggagg tcgtcaccct
    27421 gttcgatcag cccaacggga ggaacatcaa cgcgacctgc ggctcgcagc acccggaagg
    27481 tttggcccag gagatcctgc gacaaaaggc cgacgtgggg ttcgccttcg acggcgacgg
    27541 cgaccgctgc atcgcggtgg acgagacggg cacggtcctg accggcgacc agatcatcgc
    27601 cctgtgcgca tacgtcatga aaaaggaagg gaccctgacg aacaaccttg tcgtgcggac
    27661 cgtcatgagc aacatcgggc tgaccgtggc tttcgaaaag ctcggcatcg actcgatcat
    27721 gaccagggtg ggggaccgtt acgtcctgga agagatgcag gcccgcggat ccgccatcgg
    27781 cggcgaggat tccggacaca tcatctatct cagggagcac actaccgggg acggcattct
    27841 gaccgcgctg caggtcgtcg ccgcaatgaa aaaggagagc aaaccccttt ccgaactggc
    27901 ccgggtcatg accgtctttc cgcagtccct gatcaacgtg gatacgaagt ctcgtccgga
    27961 gatcgcgacg gttccgcaaa tcatgactgt catccgggag gtggaggcgg gcctgggaag
    28021 ccaggggcgc gtgctcgtcc gatattccgg cacccaaaac atgtgccggg ttatggtgga
    28081 ggggccgacg ccggaagaga cggaccgggc ctgccggcag atcgccgacg ccgtaaaggc
    28141 cgccatcgga tagccgatcc ggaacggggc ccgggcaccg tccggacgtt ctcagccatc
    28201 gtccggggcg caccgggcgg ggaggggtga catccgcacg aaccgagggg gggtacttta
    28261 tcatgccgtt tacggtcatc atcacccgtg actttgacca gatgagtgaa gtggccgccg
    28321 agatcgtcat tcagcggatc caggagcggc tacaggagaa ggcctcctgc atcctgggcc
    28381 tcgccacggg aaacagcccg accgggcttt acaagcatct ggccaaggcc gtcaactccg
    28441 ggaacatcga cgccgcccgg atccgcacgt tcaacctcga cgagtatatc ggtcttccgg
    28501 gggaaaacgc gcagcagagg gccctccatc cggagggcta cagctttttc atgatccagg
    28561 aactcttcgg gcttctgcaa agaaagtttg gcgcgacata tgtcccctgg ggcaccctgg
    28621 tggatcagga acggctggcg gcggagctgg cggaccaccc cgaagactgg tcggaacagg
    28681 gcgaagaccg gggccgggcc atcgttatcc gcgccgatgc gcgctcggac tacctgaagt
    28741 ggatccgccg cgagattctc gacgcctacc gcgaaaaaat cgaggcggac ggcggcatcg
    28801 atctgcacgt catcggcgtg ggcggtcgag gccacgtcgc ctttcacgag tccggcatcc
    28861 ccttcgagga taacaccatg ctcctcgtcc gtctggacga caacaccgtg gccaacgccg
    28921 ttgccgacgg gcacttccgc tcccggaagg aaagcccctg ctacgccgtc tccatgggcg
    28981 cggagctcgt ctaccgcgcc ggcaccgttc tcctcctcgc caacggaacg cgcaagacgg
    29041 cgcccgtcgc tgactctctc ctgaacgacc cgacgccgct cgtccccatc tcctacgggc
    29101 agatctacgc ccgtcggggg ggagacctga tttacgtcct ggaccgcgcc gccgccgccc
    29161 ggatcctgga gcagccggag cgcctccggg agcgggggat ccggctcgaa gatatcagcg
    29221 cggacggcgc gaaggtccgg gtgcgggaat tgacgtttgc ccgcgatccg gagatggggc
    29281 agatgggctg acgctcagac gtggtttcgt agcatgagct ccagcggatg ggggttcagg
    29341 taaacctgtc ttttcagata gggctcgtca taccgccgaa cgtagtgacc caggagcgtc
    29401 aggggaacga cgaggggcac gagtccccga tgataccggt cgatgacgtt tcggagttcc
    29461 tccttttcgt ccgccgtcaa tcgcttcttg aagtatcccg ccatatgatc gaggacgttc
    29521 gtgttcttgc gcgtcgtcgc cggcagtttc agcccctcca tcatgccccg cagataggcc
    29581 tcgcggagcg cccgcagcgg acggcgctcc ggattggcga ccagacggcc cagcagggtg
    29641 acgtgtttcg ggctgtggga gagaagcagg agcttgtgtt cggcgtgaaa ccggactagg
    29701 tccgccatcc ggcccccgtc ccgccagagt tcccgccagc ggtggtagac gaagatccgc
    29761 tccaggaagg tctcccggag ggacgggtca tgcaggcggc cgtcttcctc caccggcagt
    29821 agcggaaacc gctccatcag tgccgcggcg aaaatgccca cgcccttctt gaccggcgag
    29881 cccccggtgt ccggatagac cttcacggcc gccatgccgg aactcggaga tttgctcttg
    29941 aaaacgaagc cgccgagacc ctcgcgctcc agggcggaga gccgttcccg ggcccagcgt
    30001 ttcatgccgt ccgtgtggtc cacggcggtg cggatcgtga ccaggcgggg ccgctccgga
    30061 tcgccgacga ggcgcatggc ctcgcggggg acgccgaggc cgtactcgac ctccgggcag
    30121 accggcaccc agtcgaaata gcgggacagg gtgtgcgtca ggtagcgatc ctcttggtga
    30181 ccgccgtcat accggacatg cttccccaga agacaggtgc tgataccgac ccggatcttt
    30241 tccaaaggcg tccctattgc gaacccggcc gctcccgcgg ctcggggtca cgctccgcgg
    30301 tcgtccgccg gcgtcgttcc aaagaggcct tgcgctgttc ctcccggcat gccgcgcacc
    30361 cgtcgtcgaa accgcactgg ccctgacaag gctcatctct caacggcatg ggcccgcgtg
    30421 aacctcacga ctccttaaga tagaactgca tcttgagatc gccgacctcc accatgtctc
    30481 catccttgag atcacagcgc tgctcgacct tcttgccgtt caccttcagg tccttgccgc
    30541 ccgaaggatt gatgaaatac ccgtcctttc gacggttcac gagggcggcc accttcgggg
    30601 cgaaaaaccc cttgagcttg atcagggccc cgtcgtcctt gccgatcgtg gtgatccggt
    30661 ccttcaggag ataatccttc ttctccgtca ccccctgcac gatgagaaag ccccccagca
    30721 cgtcgagctt ttccgtcgac accaggatct tcttctgctc ggtgggcgac agcaccatcg
    30781 tttcgtccat cgaatggccc cggagcgccg gtttcttgac ctggtcctct cccggctttt
    30841 cggaaaagta ctccagcgtg tggttaccga tcaggatgac atccccgttg ttcaggatat
    30901 atttggacac ctttttcccg ttgatgaagg tcccgttgag gctgttgaga tcctcgacgc
    30961 tgaaatcctg ttcctctttc acgatccggg catgggatcc ggaaacggcc tgattgtcga
    31021 tcacgaggtc atttccttct ttcctgccga tggtcaggat gtcctgcgtc aatggcacct
    31081 ccttgatgac ggcatcctta aacttgacct ggatcttcgc catgcttcac ctcctgcgcc
    31141 ccctgaagaa attgatgaat gatgcaagcc aaccggtgtt ggccagataa gccacgacaa
    31201 cggtgatatt gtccctgccg ccgttctcat tggcgatctt caccaacgtc tggcaggcaa
    31261 gtgccggatc gcgggtggaa aggacaatcg tcaaaatgtc gtcatccgaa accatgttgc
    31321 tcaggccgtc ggaacaaagg accaggacat cgtcggccag gagggtcatc tcctccagat
    31381 ccacctcgac atcggcttcg gtcccgaggg cccgggtcag gatattcttg atttccgatc
    31441 gatccgcttc ctcgcgggtg atcaggtccc gcttgacctg ttcggccacc acggagtgat
    31501 cgtcggtcag ctgctcaatg ttcccgtacc ggatgagata cagccggctg tctccgacat
    31561 gggcaatgct gagcttgttt ttccggatca gcgcggccac gacggtcgtt cccatgccct
    31621 gccactgggc attgctctcg gaggcttcgt agacggccat attggccaga cgaaccgccg
    31681 aaccgagccg gttggtcaac tcggaacgat cctcgtcgta gtccccgacc tggggggacg
    31741 gccccgtacc cgcgtcggcg aaatagcccc ggatcagatc catggacatc ttgctcgcca
    31801 cctcgcccga ggcgtgcccg cccatgccgt ccgccaccgc cagcagcccc tgttccggca
    31861 ggaggcaaaa ggcgtcctcg ttgttcgccc gaacccgccc gacatccgtt tgcccggcgg
    31921 cgcgcagctc aaataccttc atgagacgac cttcatcagt tcctgaagat catcgaccaa
    31981 ctccttcccg tgctgataac gctgcgactt gtcctttgcg aggagcttgc cgatgatcgt
    32041 ttcgaactgc tccggcaaat ccggagccat ctctcgcacc gacggcggcg gcgtcgtcgt
    32101 gatgttgaag atgagggtgg cgatgctgtc gccggcaaac ggtttctcgc ccgtgagaag
    32161 ctcaaaaaac acaaccccca gggaaaagag gtcggaggcg ccgtccacct tctggcccgc
    32221 gatctgctcc ggcgacatgt agcttggcgt ccccatgatg acgccggttt gcgtcttgga
    32281 actcgccatg acccgcgcaa taccgaaatc cgccaccttg atttccccgt tgttcaggat
    32341 catgatgttc gccggtttga tatcccgatg cacgattccg ctggcatggg cgtaatcgag
    32401 cgcgccggcc acggaggaga tgatgcggac gacttcagcc cggggcagga gttgatcctt
    32461 ctggcagttc ttggccagat ccgcgccgtc cagcagctcc atggccatgt aggcgatctc
    32521 gtaatcctcg cccacgtcgt agatggtgac gatgttcggg tgggccagct tgccggcggc
    32581 ctccgcctcc cggaaaaacc gcgacttgat gtcggcgagc tgttcctcgt cgaactcctc
    32641 gtagcgcagg gtcttgatgg ccacttcccg gttgatcttg ggatcctttc cgagatacac
    32701 ggtccccatg gccccgcggc cgagttcctt catgatctcg taacggccca gggtcggacg
    32761 ggtctgggcg ttttccacga tgaccgtggt atccttccgg gcaccgcccg cgccgaagat
    32821 caccgtatcg cccacggcct tgagtttctt gatccgctcc tcgatgtcct tgaagctccc
    32881 ggccgttacc acgtgttcgt acacggctac cgccttgttg aacatccgtt tccgctcgaa
    32941 atccagaccg aggttgtaga gcagttcctt gacggcctca tcctccacgg gacatttgcg
    33001 gaacttttca aaagccatgt ccagcatgcc ctgcccttgg aaggagagtc cgagcatctt
    33061 gttcgtctcc acgctgtccg cctcgatccg ctccttcgat ttctccgtga agaggtatcc
    33121 ccgtgaaacg acgatcgtgt atcccacgag cagaagcagc gtcggataga agaccttgag
    33181 ccaaatcccc tccgccagca tgaaataggc cgccgtcacc agccaggcca cgagcaggac
    33241 ggacgagatg agggcgctga ttcccgcctt cagatggggg atccccaagg ccaggaacaa
    33301 accgaacagg atcagaatgc cgatctccag cagcgtcgcc caaccgggac ggacgatatg
    33361 gtcgttgttc aggatgttgc tgatggcgtt cgcggtgatg agcacggagg gcatggtcgt
    33421 accgatgggc gtcacctgca tcgtccccag acccgtcgcc gtcggggcga tgatgacgat
    33481 cttgttgcgg aacgattcgg gcgccacgcg gttgttgagg acctcgacga agggataggc
    33541 ctcataactg ccgctgtagc tgatgagcat ccgaccctgt tcgtggatcg ggatgcggat
    33601 cctccgaaac gtgagcgctt cgccgatcac catatctttc agatcatacc cgaggtactc
    33661 gagggacagc tggaggctca gagaagggaa gacccggccg ccgtagtcga tcaggaggca
    33721 atcgtttcgc acggcgccgt cgctgtcagc ggcgaggttg atgtgtccca gggccagtgc
    33781 cgggtctgaa aattcgggaa cgggcgggat gagttcccgg gccgtcacca agtcgggcgg
    33841 actggcaaaa gaaacggagt tgcgcgcgag cgcctcggcc atgccggcgc tgtccatcag
    33901 gacgggaccg ccgagaacga agaaaaaggg cagaacgacc cgctgggaag ccgcgatcgc
    33961 cgtgctcagc gccgcgtcat ggtccaggtt tttctcggcc gtcttcagcg tggcgtagat
    34021 ttccgcgacc gatgcactgc tctgcagccc cggcttcgcc tcgatggagg cagtgacatc
    34081 ccggatggcc tgcaatcccc ggtttgtgtc ccgttcggaa tacaggatat tgaggccgat
    34141 gaccttggcg ccgtatcctt ggagaaggtc aatcatccgg gcgatatggc tccgcggcca
    34201 cggccagcga cccagggcgt tgatgctgct gtcgtcgatg gcaacgacga cgaccggcga
    34261 ggcggtcggc ccctcccgga ggttgatccc cagatcgtag aatttgtatt ccagaaactc
    34321 gaccggcgac cagcggaggg catacacacc gaggatcaac agggtgagaa tgacaccgat
    34381 aaacacatcg gtccgaaaga ccttcgtttt tttcatcacg tttttacctg gttttcagtc
    34441 aatagcctct gaacccggga accgtgcagg cgggtgcctc ctccctctct cgcccccccg
    34501 gcggctccgc catccatggc ttggaaacgc gccgcccgac cggacggcga ccggaagtgc
    34561 tccctccgac gtccgatggc tgtcacgcta aataaaatcc tttttcttgt caacaatttt
    34621 cattcatttt tcgttcagtt cggggatcag cccttcgaac cgctcatccc gacctcaagg
    34681 ccgccaggac cttcgggacc gtatcggccg ggctgatctt gtaccaaacc gcgaggatct
    34741 tcccgtcggc atcgatcaaa aaggccgagc ggacgattcc gaaatacgtc ttgccgtaaa
    34801 gggatttctc ctgccagacg ccatagtcct ccgccacggc gtggtccggg tcggacagga
    34861 ggggaaaact cagcttcagc ctgtcgctga acgtcttctg cgccgcgacg gaatcgggac
    34921 tgatccccac ggcgccggct cccagggcgt cgagttcggc ccgtgcgtcc cgcacggcct
    34981 cggcctgctt cgttcacccg gaggtcccgg ctttgggata gaaatagacg agaagcttcc
    35041 gtcccttgaa gtccgaccgt ttcaccatga tgtccgccgc atccctaagg gaaaaggccg
    35101 gggccgcgtc accgacgttg agcttcgcca tcgtccgcct cctgtggtcc gccgacgata
    35161 agcccggcgg ggctggggga atgcctagac gatccccgaa atagttccgg cgtcgtcaat
    35221 atcgatgtgg tacgcggccg gcgatttcga caggcccggc atgcgcagga tctcaccggt
    35281 gatggggatc aggaaccccg ccccggaggc gatcttgatt tcccgcacgg tgacaatgaa
    35341 gtcccgcggc atgccgagga gactcggatt gtccgacagg gactgctggg tcttggcgat
    35401 gcagacgggc agtttgtcaa agccgaacct gcggatgagg tccaggttac gcttggccag
    35461 cggccggtag tcgatggaca ccgcgccgta gatctcactg gcaatcgtga agatcttgtc
    35521 ctccaccggc tgctcccagt catacagggt gcggaacgtc ccggtcgcgc ctgcgatgag
    35581 ccggaccgtc ttttccgcga gatccaggcc gccctcgccg cccagccgga agatgtccgc
    35641 cggcgcgata ttcatcccct cgcgctccgc ggcgtccacc accgcccgga cctcctcgtc
    35701 cgtgtcggac ggaaaccggt tcagggcgat gatgcagggg acgttgaatt tgtcgatatt
    35761 tccgtaatgc ttcctcaaat tcgccatgcc caggaccgcc gcccgggcat tcggtgcgtt
    35821 caggtcctcg cgggccacgc cggcgtggta cttgagggcg cgcaccgtgg ccaccaggac
    35881 gacgatccgg gggttgagtt cgccgtaggg cgccacgatg tcgaagaact tctccgcgcc
    35941 caagtcgaag ccgaagcccg cttccgtcac cacgtaatcc gccaggcgga gcgcgagatc
    36001 ggtgcccatg acgctgttgg tcccctgggc gatgttggcg aagggcccgc catgaatgat
    36061 ggccggcacc ccttccgtcg tctgcaccag gttgggaagc agggcgtact tcagtagggc
    36121 cgtgaccgcg ccttcgacct tgaggtcgcc cgcgatgacg ggcttgtcgt cataggtgaa
    36181 gcccaccaga atcttgcgga tcttgtcctt caattccgca taggacctcg acaggcagag
    36241 gatggccatg atttcgctcg acgggacgat gtcgaacccc gtctcgcggg gaacgccgtt
    36301 cgtcgatccc ccgaggccga tcacgatgtc cctgagcgac cggtcgttca tgtcgaccac
    36361 gcggcgccag gtgatctttc gggggtctat gtttaaggga ttgccgtgga agacggcgtt
    36421 gtccacgagg gccgacagga ggttgtgcgc gctttccacc gccgggaagt cgttggtgaa
    36481 gtggaggttg atgtcgtcca cgggcaggac gcgggacagg ccgccgccgg tcgccccccc
    36541 cttgatgccg aacaccggcc ccagcgacgg ctcccggagc gccgcgatcg tggacttccc
    36601 caaccgggcc aaagcctgcg cgaggccgat cgacaccgtt gtcttgcctt cgcccgcggg
    36661 tgtgggcgtc atggccgaga cgaggatcag gttcgaatcg tcccggcgag gaaagcgccg
    36721 gatcgcctcg aggcggacct tcgccttgta gcggccgtaa tattccagat cctcttcccg
    36781 aagaccgatg gtggacgcga tgtcccggat cggccggagc cgctcgggga cggatggttt
    36841 ttccgaagtt cccatggtta tttgttgatc accgcgtcga accggatgtc gatgttgtac
    36901 tcgtcgctga tgcggttgag gtcgtttttc agttcgagga tcttcaccgc gggcggcagg
    36961 gaaaagtagc agatcatctg gaacacgttg gcgcccgtca ccggattgtt gtccacgttg
    37021 gttgcgatgt cgtcgatgct gatcccgcgc tgctggaacg tcttgctgat ctggtggatg
    37081 atgcccgggc ggtcgatggc cgtcgcgatg agcttgtagg ggatcgacgg caggacctcc
    37141 cggtgactgg gcgccttggt cttccgcacg tagacgtcca gccccgtttt ctttcggagc
    37201 gtgtccagat cgccgatcag acgttcgatg tcgtccgttt tccccgacgt gaggatgatc
    37261 atgccgaact caccccccag gacgcagccg cgcgcgcgct cgatgttgat gccgcgcgcc
    37321 gtgaagaatt ccgcaaactc cttggccaga cccgggcggt cgggccccat ggatgaaaaa
    37381 acgacgtagg atctcatgac gtcacccccc tgcatgtcct gtttgggcat tccgatgttt
    37441 gctgtcccct cccctatccc tgttccggta catttttcaa gtgaaaagta cgtgcctcgg
    37501 tgcgacggcc atcgatcccg tttgacaatc cccctcccga tgcgtataaa tccctcaata
    37561 ggggatgatg ggagttcgac gccgaacgga cctcatccgt cgtcggcggc gcggttctga
    37621 tccgccgccg cttcattcgg gaaaaggagg gcagcatgaa ggcatccttg aaactcaacc
    37681 ggcggggctt tctcacggcc acgggtggag cggccctgat cggcgcggcg agcgcgctgg
    37741 gaacgccccg cgaggcgatg gccggccggg gaaggctcgc gacgcttatc gacctgaccc
    37801 gttgcaacgg ctgcgacggg cgggacgtcg cggcctgcgt ttccgcctgc aagacggcca
    37861 aggccggcgt tgtcccgccg cccgtggatc cgatccccgt tccctttccg acgcgcaagg
    37921 tcgaggactg gtccaagcga caggcggtgt cggaccgtct cacgccgtac aacctcattt
    37981 acatccagaa ggcggaagtg gatacgggcg gacggaagac gacggtgttc atcccgcggc
    38041 ggtgcatgca ctgcgacaac ccggcctgtg ccacgctttg ccccttttcc tccaaccaca
    38101 agttcgaaaa cggagcggtc gtcatcgacc aggatacctg cttcggcggc gccaagtgca
    38161 agacggtctg cccctgggag atcccgcagc gacaatccgg gatcgggatc tatctcaagg
    38221 tcctgccgac gctgatggga aacggggtta tgtacaaatg cgatctctgc aatgacctta
    38281 tcgaagcggg gaagacaccg gcctgcatcg cggcctgtcc aaggcaggcc atggccatcg
    38341 ggccgcggga ggagattttc gcccgggcgg aggcgctggc gaaacggatc gggggccacc
    38401 tttacggcaa ggaggaaaac ggcggcacct cgaccctcta catttccccc gtgcccttcg
    38461 ccgacctgaa cgccgccgtt cagaccggtc ccggccgacc ccaccttcag ccggttgcac
    38521 ggcgcatggc gggctcggac ggcctgggca aggtcgtcct cgcggccccg gccttgggcg
    38581 tggcggcggg tatcgtcggg gtcttcgccg cgatctccag acgcaaagac cgatcgggaa
    38641 aggaggaata ggccatgtcc caggatatcc gtgaagcgga aaaccgcgtg gtccggcact
    38701 ccggactcga aatggccgag cactgggcga tcgcactctc cgggctcgtc ctccttttgt
    38761 cggggatgtt cgaactcccg atggccgcgc gctaccggat caccaccctt cccggcctcg
    38821 cctggtcggg ggatttcatc acctcgatct acgtccacta caccgcctcg gtgatcttca
    38881 tcgccgccgc gtgctttcac ctggcctacc acgccctccg gggcgaggcc gggcttatgc
    38941 cgaaaaaagg tgatttgcgc gcctccatcg ccgtgatcaa aaccttcttc ggcaaggggg
    39001 aggagccgcc cttcgggaaa tacctccccg aacagcgcct cgcctacctc gggatggccg
    39061 tgatcatcgc cgttctgatc ctctccggcc tggtcaagac ctacaagaac gtcttcgcgc
    39121 ccgacctcga ctatacgctc gtcctctggg caacgtggct gcacaacgcc ttcttcgtcc
    39181 tgttcatcct ggccttcgtc gcacacatcg gggcgctgct gctgcgtccg aactggccga
    39241 tgatccgggg gatcttcacg ggaaccgtgc gtctggacta cgcccggcgc cgccatcccc
    39301 tctggatagc aacgctggaa ccgccgcctg ccccgccgga agcggaggag gaaccgcctg
    39361 cgcccccacc tgttcctccg gaagcgaccc cggggatgac accggaaacc gtcgccaccg
    39421 aaaccgagac ggcactcccg acaactcctc cagaggggga gggggtggtg gcgcctccgg
    39481 cggacgaacc ggacagcgcc acgcggacct gacgcgcttc acccggggtc tttgtcggcg
    39541 aggagcgatg gacgctcgcg cccccgggtc gaaatccgcc ttcaggtcaa gtctctcagg
    39601 tatggcttga acagccagcg gatcaacgag aggaggccga tgctcaggac aacggcaatg
    39661 agcacatgga gggaaatacc ggtcatcgcc acaccgaggg cggttccggc ggccggggga
    39721 tgctccgtat ccgtgacgac catcacaaag atggaagccc cgacggcaag agagtagatc
    39781 aggatggacg agaacatgac cgggtggggg atcagggaaa acaagaatcc gcagaacagg
    39841 ccgaccactt gccccccgat gacatttctc gcctgggcgg ttacatagtc aggcatcgcg
    39901 aacacgatga acgccgaagc gccgatggag gcgatgatga ccgcatgctg gaggctcaga
    39961 aaataaagga cagcaaacac ggacaacgtc gccaaaaggc tctgtagcac gtaatgcttc
    40021 cagtatcgcc tgaattcccc gtcaatcttg atcaatgttg ttcgcaagcg acccccctga
    40081 cctgtgtgtg agcgttgacc cggggaagca gaactcccga cagaccccgg gattcccccc
    40141 ttacggaacc gttccgcggc ggcgcccggg cacggcggcc agccgcggcc cccatcttgc
    40201 atatttcccg gatctcatca acaaaagact ttgagaaggc ttctgccgag caatatcggc
    40261 agattccgac gttgctttac gctgggcttt ctgataaaaa gcccgcatta cgtgagacgg
    40321 gaacagccat gctcaaccgc atcgaaatcg ggtttcggga agggatccgg gacgccctgg
    40381 gtgaaaagat ccggagacgg atcgtcgaac acttgggtct ggccgtggat cgggtccgga
    40441 cggtggacgt ttacaccatc gagggcgatc tgacgacggc ggaactggag gcggcggccg
    40501 cggggccgct ctccgatccg atcatccaga cctggagcgt caatcgcgcc ctcgccgatc
    40561 ggttcgactg gctcgtcgag gtgggcttcc gccccggcgt cacggacaac gtcggcaaga
    40621 cggcgggcga ggccatccgt ctcctcctcg gccgcgcaga cgccgttttc gtgcgggtct
    40681 acacctcgcg gcagtacatc ctgaacggcc cgctcggtcg ggcggaggcc gaaaggatcg
    40741 ccgcggacct tctcgccaac gacctcatcg accggacgca catcttcgac ggaaagacct
    40801 gggatcccgc ccgtggcgtc gccccctgcg caccgaaggt cgcgggcgaa gaccgacccc
    40861 ggctggagga aatcgacctc gacgtcgacg atgcggcgct tctcgggatc agcagcgccc
    40921 gcgtgctcgc cctgaacctg gaagaaatgc gcaagctccg cgaccacctg aaagatccgg
    40981 acgtccttcg agaacgggca cgcgtcgggc tgggcgcccg aatgaccgat gcggagctgg
    41041 agtgcctcgc gcagacctgg tcggaacact gcaagcacaa gatcttcaac gcccggatca
    41101 gctacgacga cgggacgggc cgcgtccgcg tcatcgattc cctcttcgac acgtacgtca
    41161 agggggcgac ccgcgccgtc cgggcgcgga tgggcgaaag cgactggtgc ctgtcggtct
    41221 tcgtcgacaa cgccggcgtc atccggttcg acgaacactg gaacctcgtc ttcaaggtgg
    41281 agacccacaa ctccccgtcc gcgctggacc cctatggcgg ggcgctcacc ggcatcgtcg
    41341 gcgtcaaccg cgatccgttc ggcacgggcc gcggggcaaa actgatcttc aacacggacg
    41401 tcttctgctt cgcgcccccg gattacgcgc aacccctgcc gccccggatc atgcacccgc
    41461 ggcgcatcta cgagggcgtc cgcgagggcg tggagcacgg cggcaacaag agcggcatcc
    41521 ccacggtgaa cggctgcctc gtcttcgatg accgctatct cggcaagccc ctggtctact
    41581 gcggcaccgg cgcggtcatg cccgcccggc tcgacggaca gccgacccat gtcaaggaga
    41641 tcctccccgg cgaccggatc gtcatgacgg gcggccggat cggaaaggac ggcatccacg
    41701 gggcaacctt ttcctccgag gagcttcacg agagctcccc cgtcacggcc gtccagatcg
    41761 gcgacccgat cacccaaaag aagatgacgg atttccttct cgtcgcccgg gaccgcaacc
    41821 tctaccgctg tctcacggac aacggcgcgg gcgggctctc ctcctccgtg ggcgagacgg
    41881 cccaactcgc cggcggctgc gacctggacc tcgcgaaggc gcccctgaag tacgccggcc
    41941 tcgacccctg ggagatcctc atctccgaat cccaggagcg gatgaccgtc gcggtgcctc
    42001 cggagaagat cggcgccttc ctggcgctcg ccgcgaagat ggacgtggag gccaccgacc
    42061 tggggtgcta caccgactcg ggccgctttc acatccggta cggcgacaaa accgtggcat
    42121 tcctcgacat ggacttcctc cacgacggcg tcccccagct caagctctcg gcctgctgga
    42181 cgccgccggt ccacgcggag ccggacttcc cggagccagc ggacctgggg acggccctga
    42241 aggcgatgct cgcgcggctc aacatctgca gcaaggagtc cgtcgtccgc cagtacgacc
    42301 acgaggtcca gggggggagt gtcgtcaagc ccctggtcgg cgccgcgaac gatggtccat
    42361 cggacgccgc cgtgatccgg cccttcctcg acagcttcgc gggtgtggtc gtggccaacg
    42421 ggatctgccc ccgctatagc gatctcgatg cctaccaaat ggcggcggcg gccatcgatg
    42481 aggcggtccg caacgccgtt gccgtgggag gaacgctgga ccacctggcg ggtctggaca
    42541 acttctgctg gtgcgacccg gtggaatccg aaaagacgcc ggacggcgcc tacaagctcg
    42601 cccagctcgt ccgggcgaac gaggcccttt acgacgtgac gacctgctac ggcgtgccgt
    42661 gcatctccgg caaggacagc atgaaaaacg actacgccat cggcaacacg aagatctcga
    42721 tcccgccgac cctgctgttc tccgtcttcg ggcggatcga cgatgtccgc cgggcggtga
    42781 cgatggacgc caagaaaccg ggcgaccttg tttatatcct gggaatgacc tacaatgaac
    42841 tcggcggatc ggagtggtat gccctgaacg gttttgtcgg aaacaccgtc ccccgggtcc
    42901 gtccggaaac ggccaaggcc cgctacgacg tgctggcccg ggctatccga ctgggtctcg
    42961 tcgcctcgtg ccacgactgc gcggacggcg gcctgggcgt cgccctggcc gagacggcgt
    43021 ttgccggggg gatgggaatg gacgtcgatc tcgcaaaagt cccggcggag gccgtcgggc
    43081 ggaacgacgt cctgatcttc tcggaaacgc cgagccggtt cctcgtgacg gtctccccgg
    43141 accgggagtc ggccttcata gaacagtttc gggggcagac cgccgcccgg atcggcacgg
    43201 tcacgacgga cgggcgtctc cgcctgcggg gattgcgggg ggaggccctc ctgagcgagg
    43261 agatcggcgc cctcaaggcc gcctggcagc gcccgttgaa tttctgaagg ggcgccccgc
    43321 ggcgggacag ggtcgaaaga ggtctggcgc gcgaagggag cgcgattctc aagggtctca
    43381 aagggtggag aagcaaacga tatggcccca acggtcaaat gcatcgtcct taccggcaac
    43441 ggcaccaact gcgagatgga gatggcccac gcctgccgtc tcgccggcgc cgaggtcgtg
    43501 gagatcgtcc acctgagcga actcctctgc ggggagaagc gcctggagga ctatcatttc
    43561 ctgaatctgc cggggggatt cctggacggg gacgacctcg gttcggccaa ggccggggcc
    43621 aaccggatcc tgcatgcaaa aatccgcggc cgcgcgggag cgacgaacgc cgagacgcga
    43681 ctctacgacc agttttcccg cttcatcgag gacggaaagt taatcctggg ggtctgcaac
    43741 ggctttcagc tcctcgtcaa gctcggggtg ctccccgcct tcgacgcggc ggagcgggcc
    43801 cagaccgtca cgctgacgta caacgattcg ggtcgtttcg aggatcgatg ggtccatctc
    43861 aaggtcgatc ccgcctcccc ctgcgtctat acggaaggcc tgaccggcct ttacctcccc
    43921 gtccgccacg gggaagggaa gttcatcccc ggaagcgcgg aagttctggc gaggcttcac
    43981 cgggagcact gcattgcgct gcagtacagt acggccgata ccgccaccgc cacgctcgac
    44041 taccccgcca accccaacgg ttcggtcgac gccgtcgccg gcatctgcag cgcctcgggc
    44101 cgcatcttcg gtatgatgcc ccaccccgag gcctacctcc atcgtacgaa ccacccccgc
    44161 tggacccggg aagatctgcc ggaaacgggg atgggactcg ccctgttccg gaacgccgtc
    44221 tcgttcatca aacgtcaacg cttgtaggcc ggcacgccgc gccgcgcccc caaacgaaac
    44281 cgaagacccc aggagggaag atccatggcc gaacgccccg tgtatatgac cagcttcgat
    44341 atggagcggc tcgagagcct cattcagacc caccgttcgt cgtcgccgaa gcggaaagcg
    44401 cagattgacc ggctggagaa ggaactcgac cgggccgtga tcgtcgatcc gaaggatatc
    44461 ccggccgacg tggtcacgat gaataccaaa ctgcgcctgc gggacgaaac ctccggggaa
    44521 gaaatggtcc tgtcgctggt ctttcccgcg gatgccaacc tggagaagaa ccgggtttcc
    44581 gtgctcgccc cgatcggcac ggcgctgttg ggctatcggg tgggcgacat catcgaatgg
    44641 gaggtcccgt cggggacgaa gaccttccgg atcaccgaaa ctctttacca gccggaggcc
    44701 tccggccact acgacgtata gccgatggat cccgatggtc tccccgcccg ggcgggacag
    44761 gccgatgcgc tccgtccgcg cccgttgcgg cggtgggtcg acggatcagt ccgtgatgcc
    44821 tttgtgcgcc agcgccgcgg cgatgaagcg gctgaacagc gggtggggcg ccgtgggacg
    44881 ggacttgaat tccgggtgga actggcagcc cagaaaccag ggatggtcgc ccaattccac
    44941 gatctctgcc aggcgcccgt ccggcgacac gccggtgatg ttcagcccct tctgaacaag
    45001 cagatccttg aagtcattgt tgaattcata gcggtggcga tggcgctcac tgatctcgcc
    45061 ctcaccgtag gcggcaaagg ccagggactc cgcatcgagc acgcagggat aggcccccag
    45121 gcgcatcgag gcacccatct ccttgacagc tcgctgttcg ggcaggagat cgatgaccgg
    45181 atgcagggtg gcggcgtcga actcggagct gttcgccccg ctcagaccgc agacgtgccg
    45241 ggcgtactcg accaccgcca tctgcatgcc caggcagatg ccgaagtagg ggatccgccg
    45301 ggtccgggcg cactgcgcgg ccaggatcat cccctcgatg ccccggtcgc cgaacccgcc
    45361 ggggacaagg accccgccgg cgccgtccag caggtggccg gcgccgtcct tctcgatccg
    45421 ctcggcatcg acgaagcgca gctgcacccg gcagtcgttg gcgatcccgc cgtggatgag
    45481 ggcctcgttc aggctcttgt aggagtccgt caggttcacg tatttcccca cgatcgcgat
    45541 ggtgacctcg tgggtggggt gggtcatcct gttcaccacg tcctcccagg cctcgagatg
    45601 gggctggccc gtccagatgt tgaggagatc gacgatttga cggtccaccc cctcgcgatg
    45661 gtaattcagc ggcacttcgt agatgcagga gacgtcccgg gcggcaaaga cggcccccac
    45721 ctcgacgttg cagaacaggg agatcttcgc cttgatgtcc tccgtcaggg actgctcgga
    45781 ccggcacagc aggatgtccg gctggatgcc gatctcccgc agggccttga cgctgtgctg
    45841 ggtgggcttc gtcttcacct cgcccgccgt cttgatgagg ggcacccacg tcaggtggat
    45901 gaagatggcg ttctggcggc cgacctcgtt gcggaactgg cggatggcct ccaggaaggg
    45961 gaggctctcg atgtcgccca cggtgccgcc gatctccacg atcgcgacat cgtatcccgc
    46021 cgcggtcccg cggatgaagt ccttgatctc gttggtgatg tgggggatga cctggacggt
    46081 cttgcccaga tagtctcccc ggcgctcctt ggtgatcacg gagaagtaca cctgccccgt
    46141 cgtcaggttg ttggccttgc tcatccgggt ggaacagaaa cgctcgtaat ggcccagatc
    46201 gagatccgtc tccgccccgt cgtccgtgac gaagacctcg ccgtgctgga aggggctcat
    46261 cgtcccgggg tccacgttga tgtagggatc gagcttctgg ttcgacaccc tcaggccccg
    46321 acactcgagc agggcagcga tggacgccgc ggccaggccc ttccccagag aggagaggac
    46381 cccgccggtg atgaagatga actttgtctt catagagact cctcttgcct gaatgtttgt
    46441 ctttttcccg cccccgcagc cggcccgacc cgggcgcgga tctgcccggc aaccacctcg
    46501 accgcttcat ccagcagaac gccgatcggg aggtcttcga ggtagcgatc ctcccaaggc
    46561 aggttgtact cgttgacgag gttcctcaga tcctggtagc ggacccccag gatctcccgc
    46621 ttctcctgcg tcgacagcgc gtcggcaaag cgcacgtgaa cgaggagcag atgccgcacc
    46681 ccgcgcgttt ccccccagag ggggatcatc accagcgggg cgccgtcgga ttttccgtag
    46741 cccgcataaa tcttcccggt gttgagaatc gtgcgcttgg ttcccatcag ggcggccgcc
    46801 ccttcggccc gggacgccat gttcagggcg acgccgccgc gctgccggat gcggatcaga
    46861 gaccgctcgt cgggacgccc ctcgccgtcc aggggctcga tgtcgtacaa ggtgtagccc
    46921 gtcacggcgg ccacggcctt ctgaatccgg accatatccg ggatgttctt gctcaccagc
    46981 cccttcagcg agaagccgag ttccttgagg agatcgaaga tcaccccccg gtggaacagc
    47041 tccttccggc tcgttccgac cgtcacggtc ttggcctggt gacggatcgc atcgatcggc
    47101 cgggacagct cgtcgacggc gtggccgagc gcgatgtcga gcagatcgac gggcgaggcc
    47161 gtctccctct cccccggaaa atcctcccag aaatcgtcca gaggcagctt gcccagggcg
    47221 tacttcaaaa gcaggacgag gtcggcgatg gtcccgacgc tcaggagggc aaacgatccg
    47281 gcccggcggc gctcctgaaa accggccgcg aaggcgcgca ccatctggcg atacgcccga
    47341 tccgccatca tctcgaagag ggcttgcccc tcccgccggc cctgcgccat ctgccggttc
    47401 agccggctcc tgaactcacg gaggaacagg gcgtccgcgt cgatccggcg ggcggcgaag
    47461 tagccccaca ggtggccggc gacggtattc aggatgaccg gcagcggcca cggggcccgc
    47521 ggaatgggga tgacggcgtc cgccgtgtcg tcgaagcggt cgtccccctc gtcgccgaag
    47581 acgaccacgg tggccttatg ggccttgaag atggccacat ccttgacgat atcgcccatg
    47641 accgcttcag gactccccgc cgcgcagacg aggatcagcg gctccgccga caggtcgatg
    47701 tgctttttgt tctccacaac gtcggtcgag atggtcttgt agcacagctc gctcaacttg
    47761 atccggacct cgtcggcggc ggccttgttg ggaccgctgc cgaccaccgc ccacgaggat
    47821 ttccggatcg ccagccgatc gacggccgcc cggatctcgt cctttctttc gaggacccgc
    47881 cgcatcatct ccggcgcccg ctccaggacg gccagttcgc ggtcgatggc ggcgtcggtc
    47941 agcgtgccca gaagctgggc caggcagagg gacaggacgc ggcccgcgac aatctgggaa
    48001 taaaaggcct tggtcgaggc cacggacatc tcgatgtccc ggccgtcgct cgtgtagaaa
    48061 acgccgtcgg ccttcgtggt gatgtccgac tgacgacggt tgacgatggc gatgacggcc
    48121 gccccccgct cccgcgccag ggccacggcg cggttggtgt ccgtcgtcgt ccccgactgg
    48181 gtgatgggga tcacgagtgc ggatgcgagc tcatcctcca gcgaaaaccc gctcaactcc
    48241 gaggccagcc gggtttcgac gacgatccgg cttcccttca gatcgcgcgc gagggcatcc
    48301 gcgacggcct ggccggccac ggccgccgtg ccgtggccga tcaccacgat ccggtcgagc
    48361 tctccccgtt cgaggcgccc gcggagcggc tgcgggagaa tatccacgcc caggttgaaa
    48421 cgcacctccg gccccgtctt cgcatccgcg gtgcggcggt atttccccag gagggttttc
    48481 cggaccgaca gaacggactc ttcgatctcc ttgaggaaat agtgggggta ggccccccgg
    48541 tcgatgtccc gggtggtgat ctccgcccgc tgaagatcgt cttccgtcag gggaaagggc
    48601 gctccgtcat aacgaagggc ccggaggccg gccagtccgc ccggcgattc ccggtcgagg
    48661 atgacgatct gtcccgccgg catcgccgcc ccacggccgc gggatggcgc ctctccctcc
    48721 atcttgagaa aggtgggggt cccctccacc agaccgtaca gttccgacga gaacaggtag
    48781 cggtcggggg cgaggccgac atagagggac tggccgctgc cgcgcagggc gaggaaaacc
    48841 tttcccggcg cgcagtcggc ggtcatggcg atggcgtgcg acccttcgaa gcccgcgacg
    48901 gcgcggcgaa aggcctcggt cagatcgtcc cccgcccgca gatatcgctc gatctcgagg
    48961 gggatgattt tcgtatcggt agtcacttcc gcggcaacgg tctctccccg cgcctccagc
    49021 gcgtccctca acgcggcgta gttgtcgatg tcgccgttca agacgacgct gatcgacccg
    49081 acggaccgcc ccggacgggg acgggttctt tccgtcccgg cgtcggaacc cgacggggcg
    49141 aagttgttga ccggatggca gttctcgacg gtaatggacc cgacggacgc ccagcgcgta
    49201 tgcgccatgg aaagctccgc cgcgctcggg tgacggacga cgagctgtaa gatctcgtcc
    49261 ccggcgattg cctgacggag gtcccgaacg ttccgcccca gttccccgat gatcgaggcc
    49321 gtcttgaacg tgaaggcaac ggtcctgccc gcttggcctg cgggatccgt gtccccgtcg
    49381 ggcggaaaca cggagatgga acccgggata agatctcccg gcgccgaccg ctttcggaac
    49441 gcctcgtcga ggttccgcgc cttcaactcc cgccgaattc ggcgccagtc ctccggatcg
    49501 gacaaggaaa aggagatctg tatccccgcc gaatcccgtc cgcgcacctc caggcggtcg
    49561 agaccgttca ggaggaagtt caggtagtgg agcttgcgaa acgcggccac gtcgacgtcg
    49621 cggcccgcac cggccagggc cgcgatccga ccgacattct ccaggacgtc cttttccagg
    49681 gaccacaccg catccttgag ggaaaccagc cggctgttga cgatctccag ggtctccgtc
    49741 cggaaccgtc cggcggcatc ctccagcagg cgctcctcat cggccaggaa cgccttcaga
    49801 tccgcgacca gccggctcag agcgtggaca cgatccgaat caaaaaaaag cgcctgaaag
    49861 aagcgtgttt ccttcaagca ccggaccagc ggctccagac cggccgggct ctgaccatcg
    49921 ggtccgcgat cgcccgcctc cggcgccaga tattcccccg ggctcacact tccttcgaga
    49981 accgcttcaa gcccgttgcc cttcagctcg agcgcgatcc gcgtcaagcg cggcaggacg
    50041 tcccggccgg gggatcgagc atcgtcctcc agcggctcgt gcgaacgaat cgccaggatg
    50101 ccggcaaaac cgcagaaaaa aaccccgttg aaaacgggga ggaggatgat ggcgggggcg
    50161 gaaacgcctt ggggatttcg tccgacataa acacgccacg ccttgagccg acgaacgata
    50221 cgggtgatca acgcgagtcc ctctgtcctt tcatttccgg ccgctttgcg gaacatggcc
    50281 gtcagcgaat ctttggatcc agcaaagcaa gacacgtacc actcgcagcc gcgaacccgc
    50341 cgtgacgggc ggcgcgaccg gaggtggagc accccttgcg gccaccgttg cggggccctg
    50401 tcttgaacgc gggaaatcaa ggccggaggc ggggtgtgcc cgaaacgaaa ttgtccctcc
    50461 agggacgggc acaacaaaaa tgtatccctc cttcagcggc actccggaag gatcgcaaga
    50521 gaccggtcca cgaccgtgtc gacgatgtcc tgcagccagg ccgttccggc cgccgctgtc
    50581 gccgcttcca gggactgcac gacggcgaga tatccggatg ttccagccgc ctcggcgatg
    50641 cgtttcacga acgcctcgcg cctctccggc gccgtcgatg cctcttcgcc cgacgtcagg
    50701 aacgccttga gccggggcca gccacccagg agggcgcgct gtggccggac gatgtccgca
    50761 catgccgtct catccacccg ggactccaga caccggatcg atgtctccat cttttcggcc
    50821 agggcctcga agcgggcgat gcgctcgacc agggccaact ccaaaacctg catcgccccg
    50881 tcgaagagcg ccgcccccag gtcctgtccc cggaaaaagg gcttgcggac gtggaggtac
    50941 cactggcgga gcgcaaggag attggcgata taatgaagat tgttgagaac gcggcggcgg
    51001 acgtcgccgt acaggccggg cgtaaagggc cggcggacat tgcggccggt cgccgacagg
    51061 agcgcgccgc cctccggaca atcgccccgc cagaccgtcc cggcggcgat gacggtcccg
    51121 tagccgatgc gcgccggtcc cacgagcccg ccctgccctc ccagaaagat cgggggggcc
    51181 ttcagcatga cgccccgggg gacgtccccc aagagggacg cggtcgcctt gtcctgctgc
    51241 ggcgtgtagt tgaagtggat ataggagctg cccacctcgc tgtggttctt tcggctggtc
    51301 cctcccgcca tgaggcagtc acagaagttg atcaggctcc ccagggtgac gaagggaaac
    51361 agaagggtct gcttgaggcc gacggtgtgg ctgccgttcg cctcctcctc caggaggcac
    51421 ccctctcgca cctgggcgcc gctcgccagg ttggcctttt ccaggaacac ggacgaattg
    51481 aaaaatcccc ccttgagctc caccccgggc ccaagcaggc aatcgttgag actgacgggg
    51541 gcttcctggc ccaggcgaca tccggcggcg atcagggtcc gggcgccgga gatccgcgtt
    51601 ccggcatgga gcacgacacc atcgcccgaa atacgcccgg gatcgacctc atcaccgatc
    51661 tccaccgtct gggggagggg tatgcgcacg ccccgggcga gcagtctctc gacctggggg
    51721 gaaaaacggt tctgctcctg catgcagttc atcccgacgt catcgcgtca cgcgcaaagc
    51781 cggccgttcc cttaatccgg ctgtccgttc atccccagtt tgttcattct gttctggaag
    51841 gtgttgcggt tcatgcccag ataggtggcg gcggcgcttt tgacgtgatt cgatcgctcc
    51901 aaggcgatct tgaacaggct tcgctccacc atggccatca cctcttcgta gagcttgctg
    51961 cggccgttcc cctcgtcgca caggagcagc gtcatgtcct gcatcttctt ctccagcagg
    52021 ttctccaccc gggagggaag cttcgccgcc tcagacgact tcgcgactcg taatgtgttc
    52081 attttcatag cggcgccttt ccgaatggga tccgcccggc cagccctatg ccggaaacaa
    52141 tttgagatag acgaggaccg ccagtaccgc cgcggccacc caggcagccg caatgttggc
    52201 ggggttccgc gtcggatttt ccgcctgctg aaggacctcc gcttcgattt tctcccggtc
    52261 gagatgcgtc gcgatccccc gggcgagatc ggcgaatcgc tcgtactcgt tggagaggat
    52321 gtgaaacccg cgaccggtgg tcagcaggag gtacaccttc ttacgcagca cgaggcagcc
    52381 gacatgcgag atgtcttccc agcgcagcgt cttgggcctc aggaattttc ggagggtgat
    52441 tccatccgcc ccgatcgaca cccgccggga gagggattcg ctccacacca gggccagggg
    52501 aatcaggacc agcgcgagaa agatccgctc ggtcggcgtc ccggcaaaaa aagaaagcgc
    52561 cagcaggccg atgatcagga tcaggaccgc cgtcagggga atcacgaacg accgcctgat
    52621 tctgtgcgtg tccccgttca ttcccggtcc gatccttttc cggcgccgcc ccgccggtat
    52681 tctccgctct tcccgccgcg tttgtgcaac agcatgacct ccccgatgac catcccccgg
    52741 tccacggcct tgcacatgtc gtaaatcgtc aggaccgcta cgctgacggc cgtcagggct
    52801 tccatctcca cccccgtcct tccgatcgtc ttcgccgtag cctcgacgct caccgtccct
    52861 tcacgggcgt ccacggcaaa gctcagatcc acgtccgtca gctccaggga atggcacagg
    52921 gggatcaggt cccccgttct tttcgccgcc atgatgccgg cgatccgggc cacctgaaag
    52981 acatcgccct tgggcatctg acccgccacg atcagggcgg ccgtctcgcg ccgcatccgg
    53041 atcgacgccc cggccgtcgc ctgcctcaag gtgggcgcct tgacgcttac atcgaccatc
    53101 cgcgcccgac cctgttcatc cagatgagat aacgttggca tgatggcctt tccgttttcc
    53161 ccccgccggg acaggcacct gcgtcccgtc gccgcggagg cggagatttg ccgaaccgcc
    53221 cctccccctg cctaccggga acggggctgc gtcgaagggt aaagaagggg tccgcgtacg
    53281 tgtggggcga aacttagcac agccccccgg cgtcgtcaag gtggatggga aaaagatttt
    53341 ccccaatata tttcttgcca aaacacaaat catcttctat aggtacctgc atcatccttg
    53401 gtcttccgtc ctcatcgcga tgcaggagga acccccttga tcgtcaaggt cttcagcagc
    53461 gccatcatcg gcgtggacgc ttaccccgtg gccgtcgagg tggacatttc tcccggcctg
    53521 ccccagttct ccaccgtagg cctgcccgac gtggcggtcc gggaaagcaa ggaccgcatc
    53581 aaggcggcca tcaagaattc gggctacccg ttcccgaaga cccacgtgac ggtcaacctg
    53641 gccccggccg acatcaagaa ggaggggacg ggattcgacc tgccgatggc cgtgggcatt
    53701 ctggccgccg atgggaccct agggcctcaa ccggcgcagg actatctcct gctgggagaa
    53761 ctgtccctgg acggatcggt caagggcgtg cccggtgttc tctccgccgc cttccaggcg
    53821 cgggcctcgg ggaaaaaagg catcatcgtc cccgttgaaa acgcccgtga agccgccatg
    53881 gttcaggatg tcggggtcat gcccgtgggg aatctggccg aggtagtcga gtttctctcc
    53941 ggccgcaggg cgatcgaacc ggtggtcgtc gacgcggcgg ccgtcttcag ccagagccac
    54001 cgatacccct tcgacttcaa ggatatcaga ggtcaggagc aggccaagcg tgccttggaa
    54061 gtggcggcgg cgggctcgca taacgtgttg atgatcggtc caccgggggc cggaaagacg
    54121 atgctcgccc agcggctgcc gaccattctc cccgacctga cgttccccga ggccgttgaa
    54181 accacgaaga tctattccgt cgcgggcctt ctggaccgga agcaggcctt tctggcgacc
    54241 cggccgtttc gcgccccgca ccacagcatc tccgatgccg ggatggtcgg cggcgggcac
    54301 gttccccacc cgggggagat cagcctcgcc caccaagggg tgctttttct cgacgagctt
    54361 ccggagtttc ggaagaacac cctcgagtcg ctgcgccagc ccctggaaga cgggacggtc
    54421 acgatcagcc gttctctgat gcggacgacc tatcccgccc gcttcatgct gatcgcagcc
    54481 atgaacccct gcgcctgcgg attctacggg gatccccgac gggcgtgccg gtgcagcccg
    54541 cggcagatcc gccagtacca ggggcggatc tccgggcccc tgctcgaccg gatcgatatc
    54601 cacatcgacg tccccgccgt ccggtaccgg gagctggcgg cccgccaaac ggccgagccc
    54661 tccgccgcca tcaaggcgcg gatcgaggcg gcccgccggt gccagcggga gcgcctcccc
    54721 gatcatcccc tcttcacgaa cacccgcctg accgacccgc aactgaaggc attctgcgcc
    54781 ctcgacaccg agtcgcagcg cctcctggag atggccgtcg accgcctggg gctctccgcc
    54841 cgggcctaca cgcgcatcct caaggtcgcc cggaccatcg ccgacctcga gagcgaagcg
    54901 gacatccggt caccccacgt cgccgaggcc atccactatc ggacccttga tcgcgcgctg
    54961 atttagtggt atgcagggaa ccgtgtagct tcacacagcc agggagatct cgaagaaaag
    55021 aggaatgtgt atggaaatca cgatcaggga gcttcccccc gaaaaaagaa aagcgaagcc
    55081 ggccgacgaa tccaagctgg gattcggaaa gttcttcacc gatcatatgt tcaccctgaa
    55141 atacgacacc gcgctcggct ggcacgatgc actgatcgga ccttaccagc ccctctccct
    55201 ggacccgacg gccatgtgcc tgcactacgg acaggagatc ttcgaagggc tgaaggccta
    55261 tcgcggtcag ggcggcgcca tttacctctt ccgtccggag gaaaacatcc gtcggatgaa
    55321 cgcctccgcc gagcggctct gcatgccccc cgtcgacccc gccctgtttc tcgacgcgct
    55381 gaaggagctg gtccgcgtgg agaaggactg gatccctcac agccggggcg cctccctgta
    55441 catccgcccc acgatgatcg ccacggagcc cgcgctcggc gtgcatccgg ccggggcata
    55501 ccttctcttc atcgtggtcg gtcccgtggg cgcttactat gcggaaggct tcgcgccgac
    55561 caagatcttc gtcagcgagg actacgtgcg cgccgtgcgg gggggcatgg ggaattgcaa
    55621 ggccgccggg aactacgcgg ccagcctcta cgccagccga atcgcatcgg acatgggtta
    55681 cacccaggtc ctgtggctgg acgccatcga acggaaatac gcagaggaag tgggaacaag
    55741 caacatcttc ttcgtcatcg gggacgagtt ggtcaccccc ccgctcgccg gatcgatcct
    55801 ccccggcgtc acgcgcaact ccatcctcca gatcgcccgc cactggggct gccgtgtggt
    55861 ggagcgaccc gtggccatcg cggaagtgat cgaggccgcc ggcagcggcg tcctgaagga
    55921 ggcctttgcc tccggaacgg cggcgatcgt ttcccccatc ggccagatct actaccaggg
    55981 caaggagcac gtgatcaatg gcggcaagac cggcgccctg acggagcggc tctacaacga
    56041 gatccttcag attcaatatg gcgagaagga cgatcccttc ggctggcgga tccagatcgc
    56101 ttagggaatg acagaccgac ccccagtgcg ctcctcatac aggggtcgat acggccgttg
    56161 atacagggac ttggcaagaa atccacaatc ctgtggagaa ggttgtggat aacgatgttt
    56221 ataactgccc taagtctgca attgcgacac ttttctacca cattgcataa ggtttgggca
    56281 acaacaattt ccaatattat cgatgagtta gattatatat cctgaaattc cttcatttat
    56341 cgcctctttc gggtatggga tattaacttt aattaaaaaa gtggcctgaa agggccgttt
    56401 ttcgaagacg caggcacgat atttgacggc ccgaaaatga aaggaccagg acgtgaacgc
    56461 tcgggggaca agaacccttc aacgggccct gctggtgatc tcgatgacgg tgctgtcact
    56521 gatcggggcc agccccgccg gggcggacct cccgcccctg tcggaggtca tcgccggcat
    56581 tcagcggcaa tacgacggca tgaccgatct tcgggcgcgc tttgtgcagg aaatcacgct
    56641 cagcgcgatg cagcggacgg accgtgagga gggaactctc tactggaaaa agccccagcg
    56701 gatgctctgg cattatcaca aaccccgggt caagaagctg atcatcaatc agcggcagac
    56761 ctggctgtat atccccgagg accaggccgc ctatacgcag aagacggggg cgctgctgaa
    56821 atcccagacg gccgttcggc ttttttccgg attttcgcgg ctgcaggaag acttcgagat
    56881 ccgtttcgcc cagccatccg ggaaggaccg ggaagggaac tatctgctcc tcctgacccc
    56941 gaaaggccgg gacacggggg cgtcggatat cgtcgtaacg gtcgatccgg agcgtcacct
    57001 ggtcatccgc tgccgctttc tggacgccta cggaaacgtc acccgtcttt cgctgaccca
    57061 ggtccagacc gaccggcgcc tccccgatcg gttgttccgc ttcgaaccgc cgccgggcgt
    57121 cgatgtgctt cccttgccgt aacgcctccc ggatcggccg gcttcagaag gacatcccgt
    57181 catgatgaac atcgcactcc ctccctggac cgaacccggc gcgaacgaat cgttcctcaa
    57241 acgggaatgg ctcgagacca acggacgggg ggggtacgcc tccagcaccc tgcttaactg
    57301 ccatacccgt aaatatcacg ggctgctcgt cgcgaacctc gcgtctcccc cgggccggta
    57361 tgtcctgctg tccaaattcg aggactcctt gcggctgcgg gatcgggaga tctatctgtc
    57421 ctgccatcag tacccgggaa cccttttccc cgacgaggtc ctgctccggg aatttatcct
    57481 ggacggttat ccctcgtttc tttacacggc gtccctcccc ggcccgggga cgaccgcggg
    57541 aatcaagggg gacaccccgg ccggcggaac gatccgcctg cgcaaatccg tcctgatgat
    57601 cgccggggag gatgccgtcc tcgtccatta cgacctggag gccgctccgg ccggcgccgt
    57661 gctgcgcctg agacccttcc tcgcctaccg gggccgtcac gaactgagcc gccggaaccc
    57721 cttcctgcgg gccgaggtac agccggcacc caatggattt ttcatgaacc cctatcaggg
    57781 catgcccccc ctgtatctcc agaccaaccg gaagggccgc ttcatcgagg cccccgcctg
    57841 gtatgaaaac tttgaatacg tccgggagca ggaacggggc tatgacgggc atgaggatct
    57901 gttcacgccc ggtatcctcg aagtgccgat tcgcaccggc caggccattc tcgtacgcgc
    57961 ggcgacgacg ccgggcaaca cggcgctgca ccgcctctgg cagcgggaag cggacgggcg
    58021 gcggacggcg acgacggaag ggaaggtttc agagccggga aggatcgccc ccccacccgg
    58081 accggaggat tcgtccgccg gcggcctggc ggccgaagat tgttcctgcc gggagcggct
    58141 tttcagggcc gcccggcgat tcctcgtgac gaccccggcc ggccggccgg ccgtgatcgc
    58201 cggctatccc tggttcggag actggggtcg tgacaccctc atcgccctgc cggggatcgc
    58261 tttctgccgc ggccggtttg aggagggcgc cgcgatcctg ggcgccctgg gggaccacga
    58321 acgggacggc ctcctgccca attatttcac cgcggacgaa agcgatccgc cctacaacac
    58381 ggtggacgcc tccctgtggt ttttctgggc ggttcagcag tacctccttt acggggggga
    58441 cccggaacgc gtccgcctga ggttctggcc cgtcatgacg cggatcctga cgcacttcct
    58501 ggacggcacg cgcctgaaca tcggcatgga cgaccggggg ttgctgcacg ccggcgacgg
    58561 gagggacgcc ctcacgtgga tggacgccca ggtcggaggc cgaccggtca cgccccggga
    58621 cggctgcgcc gtggagatca atgccctgtg gtacaacgcc ctcgccttct ccgaacagtt
    58681 gtcccaccga ttcgggaagc aggacatcga tctcgagggc atgctccgca agatccgcca
    58741 gtcgtttcag gataccttct ggctcgagtc ggaacactac ctgggcgacg tctacgccgg
    58801 gaaccggctc gatcgcgcca tccgtcccaa ccagatcctg gccgtttccc tccccttttc
    58861 tcccctggac ccgttcaaat ggaaggccgt ggtgcacacg gtgaccgacc atctgctcac
    58921 gccgtgcggc ctgcgcaccc tttcgccgga ggatcccgcg taccggggcg tttacggcgg
    58981 cgatccggcc gcacgggatg gggcgtacca ccagggaacg gtctggccct ggctccttgg
    59041 ccatttcgga gaggcgaccc tgcgggtggc cgaaaaccgg cacgccgccc ggacggttct
    59101 cctccgccat ttgcgttcat ttctgcaggc gcacctgccc gaggcgggcg tgggcggtat
    59161 ctccgaaatc ttcagcggag aagcccctca ccgtcccgac gggtgcatcg accaggcctg
    59221 gagcgtgggg gaactgatcc gcctctacga ccttctggcg cggggataac ggcaccggaa
    59281 cgacaaacgt caggatggtc acgggcgcgc gggcaccggg tttactctat gccagaggtc
    59341 actcggcaaa agcgaaaggc cctccgcgcg cgggcatcgg atggccccgg acgttcagcg
    59401 ggacttcgtc agttctccgt acttccgggt cacgacgaag atcccctggt cgaacatctg
    59461 gatggtccgg ttgatgcttt ccaccgcctg atgcatatcc aaggtggtca cgaagaccac
    59521 gttgaaatcg gcgaaaagcc agatgtggcg gcgaagcagg atgagggtgt aaacggcctc
    59581 ctccaggggg atgcccttgt tgaacatctc ccgcccgaga cgggagaaga actcatccac
    59641 ctccgggtag gggcgtgcgg agaagtacag cttccgcagg ttctcgtaga agctgatccc
    59701 ccactggacg tagtcttctt ccgacagcgc gtgatagaaa ggggttcggg ggttggagcg
    59761 tacggccttg ctccattgtc gcgcaacttc ttcggtgtgg cgttccagga tgtcgatcaa
    59821 cttgtccgcg aatgctctca tgaccccctc cttcatctct tccgtagcca ttgacaggat
    59881 gtaaaccggt ggacgcgtca ctcgctgagg cgtggctcta tattaagtct tggatgcagc
    59941 cgtgtcaaga gagcgcgggg agcgcgggtg cggggctccg gagaacggga ggcgccttcg
    60001 acatcacggg gagggtgacc gctgcacctc actcatagcg gacgaggctg tcgatccgcc
    60061 ccatcagttc cgccacggca cggttcacgt ccgccaccac cacgcgaatg gcggcctgcg
    60121 ggcagacggc ggcgcagcgg ccgcagccct tgcacaggac cgcgtcgtga acgatctccc
    60181 cgtcctccag gcggatcgcc cccatgaagc actcctccac acaggcgccg caccgcacac
    60241 acaggtccgg atcgacgctg atcgtaagcc cggccagcgg atgaatggcc gcctgggcct
    60301 cagccggcag ataacggccg gaattcatga tcgtgcagca gcaccggcag cagtaacaga
    60361 tcgtcagcag tttccctcgg tccttcaccc cccagatgaa gttgtcgatc cggacgcgtc
    60421 cggtcatggg gatgaggcct tcccgaaggg cgtcatccag atgcgccagg gcctcctcca
    60481 cggtggcgtg gcgggcgatc cggggatcga tctccttcgt cccctccccc aggagggtgc
    60541 agcccatcgt gacgggatgg cggcggcacg atttcgcgtc ccggcaggtg cagcggttga
    60601 tgatgacccg atgggcggaa cggcggatga gcgcctccac gacctggcgg ggcaggagcg
    60661 tgtttgcggt gcctccgacc tcttcgttga cggggaggta ggtaatattg aggtttttcc
    60721 cggcaaaaag gggcaggctg ccccaggcga ccacccggcc gacgacgggc catcgcgtca
    60781 tgcgcgcact gatccaggtc aagggccaga tcctggccag aacagccagc caccatgcgg
    60841 gtctgaagga catgctgcct cccttcccgg ttcgcgaacg tccgggcaac gcggctcatg
    60901 gtcaggatcc cggaatcttg atcgctccga cggacccact tgctttaaat agacgcatcc
    60961 cggtgccgtt gtcaagacct ccgcttgact ctgactcaaa actcccgaca ttaccatcgg
    61021 ggatccgaag acggaccgtc cggcgtcgag ccggggccgg gcgtattttt ccttgatttc
    61081 ccgaggcttc ttgtctatac ctcaacgcgc cgtgcacgta cgccgccgga aggacgcggc
    61141 cggggtcacg cgagcggacg aaaaccttgc gcaggggtcc gtttccggtg tacaaaaccg
    61201 tcgtgctgtt gtttttctcg aacctcttca tgacgttcgc ctggtacggc catctcaagt
    61261 acatgaacgg ccggccgctc ctggtcgtca tcctgctgag ctggggcatc gccctgttcg
    61321 agtacaccct ccaggtcccc gccaatcgca tcgggttcca ctattacaac ctcggtcagc
    61381 tgaaggtgat ccaggagatc atcaccatga cggtctttgc cggattcgtc tttctcgtca
    61441 tgaagcagcc gctcaaatgg gactacctgt gggcggggct ttgcctggtc ggcgccgcct
    61501 acttcatgtt ccgcggcggt cttccccccg cctgatcgca ttccattcgc ccttcgcgct
    61561 cttcgaagcc agcccggagc ggccgtcacg atggcggcca tccgcccatc ccgacggtcc
    61621 cggacggaac aggtcttccc ctccggaaaa tcgcttgaca cgaaacatga attcaattat
    61681 tgaaccgaat taggttcaga ttgtgaattg gataatcccg tttttcgaac cttttcggct
    61741 tcatcgatcg caaacgcttt tcagtccttg agcccccgga tccgtgaaac gatcaagggg
    61801 caattttcct gcatccgttt tccaaagatt caacccattt cgtcgaccac acggagggct
    61861 tatggaggtt atcgagacaa gaatcgatcc ccgctcggag gcgtatcgcc aaaattacga
    61921 agcgatggcg gccctggtaa cgacgctgaa ggaggaactc cggattgccc gggaggaccg
    61981 ctcgccacag gcccggaacc gtctcgcctc ccaggggaag atgcccatgc gggaaaagct
    62041 cgacctgctg ctggacagaa acaccccatt tttggagatc gccccgctgg ccgcccgggg
    62101 gctttacgac ggcaagattc acggggcggg gctggtctcc gggatcggcg tcgtcggcgg
    62161 gagggaagtc ctgatcaccg gcaacgacgc gatgatcaag gggggcgcca cctatcccct
    62221 gacggtgaag aaaagtctcc gctgccagac gatcgccatg gaaaatcgcc tgcccgcgat
    62281 caccctcatc gattcggcgg gcggctacct gcagctgcag tccgaggttt ttccggatct
    62341 cgatgacggc ggacggcttt tctataacca ggcgatcatg tccaagatgg gggttcccca
    62401 gatcacggcc gtcatggggc tctgcaccgc gggcggcgcg tacgtgcccg ccatgtccga
    62461 tgagacggtc cacgtcaagg gcacgggcgc catcttcctg ggggggccgc ccctcgtcaa
    62521 ggcggccacg ggcgaggagg tgacggcgga cgaactgggg ggcgcggacc tgcactgccg
    62581 cgaatccggc gtctccgact attatgcgga ggacgaccgg cacgccatcg ccatcctgcg
    62641 caacatcgtc gaaaccctgc cgcggacgga aaaggcccgc ctgccgatgc atgaaccggc
    62701 gccgccgctg tacgatccga aggagatcta cggcattgtc agtcgcgaac tgaaggtgcc
    62761 ctacgacgtg cgcgagctga tcgcgcggat cgtggaccgc agcgaattcc tggagttcaa
    62821 ggagctgtac gcgccgaccc tggtctgcgg ctgggcgtac ctccacggct accccgtagg
    62881 catcctgggc aacaatggcg tgctgtttcc cgacagcgcc ctgaagggga cccagttcat
    62941 ccagctgtgc gaccgacgtc agatccccct gatcttcctc caaaacatca acggattcat
    63001 catcggcagc gagtacgagc ggctcggcat cacgaaaaac ggccacaaga tggtcaacgc
    63061 cgtggccacg gcgaccgtgc ccaagttcac cgtaatcgtg ggcgcctcgt tcggggccgg
    63121 caattacgcc atgtgcggcc gggcctattc cccgcgcttt ctgtggatgt ggcccaacgc
    63181 ggaaatcgcc gtgatgggcg gggagcaggc cgccggcgtg ctcgtcaccc tgagaaacga
    63241 ccagctcgcc aaggcgggaa gaccgcccct gacggaggaa gaggcggcgc agatccggga
    63301 tccggtggtc gccgccgccc ggacggagag caacgcctac tacagcacgg cccaactgtg
    63361 ggacgacggc atcctcgatc cggccgatac ccgcgatgtg ctggggctcg cgatatccgc
    63421 ggccctgaac gccccgatcc gcgacgatgt cttcggatac ggcattttcc gcatgtaggg
    63481 cgtgacgacg actcgcaacc gctgaacggg agggcgcgca tgttcaagaa gatcctggtg
    63541 gcgaaccggg gggaaatcgc ccggcgcatc ctgcacacct gccgggagat ggggatcggc
    63601 acggtggccg tgtattccga tgcggaccgg aaggcggtcc atgtcctgga cgcggacgag
    63661 gccgtccatc tcggggcggc cgatccgtcg gagagctacc tccacatcga ccgaatcatc
    63721 gcggcggcca aggaaaccgg ggcgcaggcc gttcatccgg ggtacggatt cctggccgag
    63781 aacggggatt ttgccgagcg ctgcgagcgg gaggggctcg tgttcatcgg acctccggcg
    63841 cgcgtgatcc gcgcgctcgg ggacaagacc gtcgcacggg agacgatgat ccggaacggc
    63901 gtgcccgtca tccccggcat gaaccgcccc gaatccgatc ccgacaccct ggcgaaggag
    63961 gcggatctgc tgggctaccc agtcctgatc aaggcggccg cgggcggggg gggcaagggc
    64021 atgcggatcg ttcaggcgcg ggatgcgctt cacgaggcct gcgtgtccgc cgcaagcgag
    64081 gcctccgccg ccttcggcaa cggggccatc tatctggaaa agtacctcgc gcggccgcgg
    64141 cacatcgagt tccagatcct ggccgacggc ctgggaaata tggtgcatct cctggagcgg
    64201 gagtgctcca tccagcggcg ccaccagaaa atcatcgagg agacgccgtc gcccgccctg
    64261 acgccggagc tgagggagcg gatgggccag gcggccgtgg ccgcggctag ggcgtccggc
    64321 tacgtgaatg cggggacggt ggagtttctg ctggacccgg acggccgctt ctatttcctg
    64381 gaggtcaaca cccgcctcca ggtcgagcac ccgatcacgg agatggtgac cggcatcgac
    64441 gtcgtccggc gccagatcga gatcgccgcc ggcctccccc tgaacctcac ccaacaggag
    64501 atccgcgggc gaggccacgc catcgagtgc cgcatctacg ccgaagaccc ggagcaggga
    64561 ttctttccgt ctcccggaac tatcgtgttt atgcaggaac cctccgggcc gggcattcgg
    64621 aacgactgcg gcatctacgc cggatttcag gtgcccgtgg agtacgaccc catcctgtcg
    64681 aagctggtgg cccacggcga agaccgcgac gcggccatcg cgcgcatgct ccgggcgctc
    64741 gagcgctacg tcatcctggg gatccggacg gccatcccct tcctcatgga tgtcctccgc
    64801 tcggacgcct tccgcgccgg cgagacgttc accgatttcg tcgccgccca tttttccgac
    64861 tggaaacccg cccggacgga gacggacctg gcgcgcctcg cctacatcgc cgacgatctc
    64921 ggcggccgca gggaaaccaa ggccacgggg ctgccggcgg gggaacatcc gtcgccctgg
    64981 cagcgcctgg gcaactggca gcggtagcgg accggcgcac cgcaggacct ttcacgcagg
    65041 agaacaggac gcatgcaata ccgattcaag acggacgaag acgcggcggt ctggacggtc
    65101 gatccggaag ggaccgaccg cctgaccgtg accacgggaa cgcaaacgct tcatgtccgc
    65161 catggcgccg tctcccccca ccacctgcac ctggcggtgg acggcctcgg cgtgaacacc
    65221 tttgtcttgc ggggggacgg cgaaaagacc gtcgtgatcc gcggcgttcc gtaccccgtt
    65281 caggatgcgg atttcgccga acggaaaaca aagaaaaaag cggatcggga gaatctcccc
    65341 caggtcatca cgccgccgat gccttcggtg gtggtgcgca tcccggtgag cgagggcgac
    65401 cgggtgtgca aaggacaggc cgtcatcgtc ctgacggcca tgaagatgga gaccacgctg
    65461 acgtcgccct ttcccggccg ggtgacgaag gtcaacgttg ccgcgggcga gaaggtgatg
    65521 cccgggcgga ttctcgtgga catcgaaaag gaagcggaga ccgacgcgcc ggatgagggc
    65581 cgctaggccg caaccggcgc gccccccatg atcccgcaag gaggcaacag gatggacgaa
    65641 cggcatttgc tctaccgggt cgaggacggc atcggcacga tcacgatcaa tcgcgaggcg
    65701 cagcgcaacg ccatcacacc ggaggcgatc cggctgtttc acgtgtccct ggacgaggcg
    65761 gagcaggatg agcgcgtgcg ggtcctgacg gtcacgggcg ggggggacaa ggccttctgc
    65821 acgggagcgc agctcagcgg cgccacgacg acggaaggcc gggacctgtt cgccgattac
    65881 gccgccctgc tcgacaagct cgtccgtttt cccaagccga cggtggcgcg cgtgaagggg
    65941 ttctgcctgg ccggcgggat gggtttcatg ctggcctgcg acatcgtcat cgcgagcgac
    66001 gacgcccggt tcggcacgcc ggaggtcaac gtcggcctgt ggccgatgat gatcggcgcc
    66061 ctcatttttc gcaatgtcct gcagaagaag gccatggaga tgatcctcct gggcgagcgg
    66121 atgacggccg cccaggccat ggccatgggg ctcgtcacgc gggtcgtgcc cgcggagagg
    66181 ctggatgagg aggtggaccg ggtgctcaag gccctggcct ccaagagccc gatcgggatg
    66241 cggatcggga agcaggcgtt ctacgggatg gcgaacctgc cgctgccgga cgccctcaac
    66301 tatctttcgg aaaagctcaa ggaggtggcg gcgacggagg atgccatgga gggaatcgtg
    66361 gccttcatgg agaagcggcc gcccgttttc aaaggccgat aaaatgatca cggacagggg
    66421 ggagaaagta tgggcgcaac gggtcaacgg ggcagggacc gcgtcatcat cgccaattgc
    66481 agcgggtatt tcggcgatcg gctggccgcg gcccgcgaaa tggtgcaggg cggtcccatc
    66541 gacgtcctga cgggggatta tctggcggaa ctcacgatgg cgattctgtt cgcccagaag
    66601 atgaagaagc cggagaccgg ctatgtgccg acgtttctca ggcagatgga agaggtcatg
    66661 ggcgaatgcc tggacaaggg catccgggtg gtttccaacg ccggcggcct gaatccgcgg
    66721 ggcctggcgg cggagctgaa aaagatcgcc gacgcgctgg gcctgcatcc gaaaatcgcc
    66781 tgcatcgagg gtgacgacct catggggcgg ctggcggacc tgcaggcgca aggggaggcc
    66841 ttcatccact tggacaaggg gatcggcctg aaggacgcgg gcgccatgcc catttcggca
    66901 aacgcctatc tgggcggctg gggcgtggcg gaggcgctgg agcggggtgc ggacatcgtg
    66961 gtcgggggcc gcctggcgga cgccgcggtc gtcatgggac cggccgcctg gtggttcggc
    67021 tggcggcgag acgattggga ccggctcgcc ggcgccgccg tggccggcca tatcatcgag
    67081 tgcggcgccc aggcgacggg gggcaattac tccttcatcg acgaagtgcc ctcgtttctg
    67141 aaggtgggct accccatcgc cgaaatcttt gcggacggcg ccttcgtgat cacgaagcat
    67201 cccggcacgg gcggactcgt gtcggtcgat acggtcaagg cccagctcct ctacgaggtc
    67261 cgggacccga aatatctgac cccggacgtg gtcgcacggt tcgacacgat cgggatctcc
    67321 caggagggcc ccgaccggac gaggatcgcc ggcgtcaagg gcgaaccgcc ccccgcgacg
    67381 accaaggtct gcatcaacaa tgccggcaac taccgcaatt cgatgaccgt cgtcctcacg
    67441 ggcctcgata tcgaaaagaa ggcgaagatt ttcgaggacg ccctgttcga cgccctgggc
    67501 ggccgcgggc agttcgcggt cgagcatgtc cagctgatcc gctccgacaa ggaagacccg
    67561 cagacgaacg acgaggcctt tgcctacctg cgcctgtccg tgatggatcc ggaccgcaaa
    67621 aaggtcgcga aattttcggc caagatcgtg gagctggggc tggccaacat ccccggcttt
    67681 acggcgacgg ccccgccgac caagggcggg ccggccatcg ttcactggcc gacgctcatc
    67741 gcgacgaagc acatccggca gacggtcctg ctgggggacg agacctggac ggtggagtcg
    67801 gtgccgccgg atccctcggc gcccgcgccg gaagcgccga agatcgacct gccgccggcg
    67861 ccgcggggga agacgatccg gatgcccctg gggcgtgtct tcgccacgcg ctccggagac
    67921 aagggaggca acgccaatct cggggtctgg gccaagacgc ccgaagcctt cgctttcctc
    67981 ctggaattct tgacaaccga aaagctgaag gagcttctgc gcgacctgag cgattttgag
    68041 atggagcggc atgaattccc gaacctgctg gcggtgaatt tctatatcaa gggggtgctc
    68101 ggggaaggcg tggcggcctc ggtccgcagc gatccccagg cgaagacgct gggcgaatat
    68161 ctgcgggccc gtgtcattga gatgccggag tcgatagtgt ccaggacaaa cgcagggcgt
    68221 taggggctgg acagagacca acgcctcccg acaacatccg cagggcatgg aatttctgca
    68281 tgcagaaagg agctgtgccg tgagcgcgta cgatgccaag ccgtggctca agtcgtatga
    68341 tccttgggta acgcccgaga tgacgattcc cgacgagacc tacgtggatc tcctggaaaa
    68401 ggccatcgcc cacgacccgg atcgcgccgc gtttcatttt ctcggcacga cctgcaccta
    68461 ccggaacctg gaccggttca gcggccgtgt ggcggattat ctggccgccc gggggtgcgg
    68521 tcggggagac gtcgtgggca tcaatctgcc caacacaccc cagtatctga tcgccttggc
    68581 cggcattcta cgtgccggat gcgtcgccag cggcgtctct ccactcctga cgcccaggga
    68641 aatggcccat cagatcaacg actcgagcct caaggccctg atcacgctgg acgccatctt
    68701 cgagcagcgc gtccagaaga tcggcaacga gatcccgtcc gtgaagcatt gcttggtcac
    68761 caacatcacg gactttctcc cagcgtacaa gcgcgtgctc gccaaggccc tgaaaaaggt
    68821 tcccgtcggc cggatggcgc cgatcgcggg caaggaagtc ctccctttca tggacgtgct
    68881 ccggaaagat cagccgcccc ggcccgggat caagatcgat ccgggggatc cctgcctcat
    68941 ccagtatacg ggcggaacga cgggcctgcc gaaaggcgcg gtcctgaccc atcgcaacat
    69001 ggtggccaac atcacgcagg cggtcacctg gttcaacctc aaaatgggcg gcggggtcgc
    69061 ctgctcggga ttcccctttt tccaccaggc ggggctttat ttcggcctga tcacgatgtc
    69121 cgccgcctac acccagtgcc tgatccccga tcccagaaat acggatcacg tttgcgcgga
    69181 gattgcgaaa taccgtcccg ccatcctgct gcacgtgccc tccctctacc agatgctgat
    69241 ggacaatccc gccttcgcga agatcgattt ttcccggtgc gaggtgtgca tctccggagc
    69301 ggcccccttc tcgaaggagg ccatcacggc cctcgaagcc atcgtcggaa gggggaaggt
    69361 ggtggaaatc ctcggcatga ccgaagccag ccccgtgatc accatgaatc ccctccgggg
    69421 gcgaaagaag atcggctccg tcggcattcc gttgcagagc acccggctca agctggtgga
    69481 cgtggaaacg ggcaccttgg aggtcccggt cggagaggtt ggagaaatca tcgtgcacgg
    69541 cccccaggtg atgaaggggt atcacaacaa acccgaagat acggccaaga ccttgcggcc
    69601 cttccagaat gaaacgtggc tctacaccgg cgacgtggcc cggatggatg aagacgggta
    69661 tttctacatc gccgaccgca ccaaggacat gatcatcgtc gggggtttca aggtgttctc
    69721 ccgggaggtg gaggaggtgt tgagccagca tccggccgtc cagttctgcg ccgtcgtcgg
    69781 catgacggat cccaagcggc cggacagcca gctcgtcaag gccgtgatcc agctcgccgg
    69841 cgaggcggcg ggcagcgatg cggaagccgt ccggaaggac ctgctcgacc attgtcggga
    69901 gaacctgacg ccctacaagg tccccaaggt gatcgagatc atggcggcca tcccgttgac
    69961 cgccgtgggc aaggtcgaca agaaagcgct gagggtaaag ggctgaaggc cggggaatgg
    70021 atcccttgcc cctccccttt cgccaacctt tgcaataagg aggaacgcat gtatttcaat
    70081 gaaacgcacg aggctttccg ggcatcggtg aggagattca tcgatcggga gatcaacccc
    70141 tacatggacg aatgggagga gaagtccgtt cccctgcacg acctgttcaa gaagatgggg
    70201 gagctcggct tcctggggat ccggtacgac ccgaaatacg ggggacaggg gctcgactac
    70261 tggtacgaaa cggtgctcct cgaagaggcc ggccacattc gcgggttcgg cgtctcgacg
    70321 gcgattgccg tgcagacgaa catggcgacg cccgccatcg acgccttcgg cagcgaatac
    70381 ctgaaggaca aatacctgcg gggcgcgatc gccggcgacc tggtagcggc catcgcggtg
    70441 acggagcccg acgccggttc ggacgtcttc gccatcaaga cgcgggcggt ccgcaaaggg
    70501 gatcactacc tcctcaacgg ctccaagacc tacatcacca acgggacgca ggcggatttt
    70561 ctgacgctgc tcgcgaaaac aggggacgac gggggctacc attccttcgg cctgttcgtc
    70621 gtgccaacgg acctccccgg ctttgtcgtc agcaagaagc tcgacaagct gggtttgcgc
    70681 agcagcgaca cggcggaact ctttttcgac aacgtggcgg tgccggtgga aaatctcatc
    70741 ggcgacgagg gcgaaggctt tatctaccag atgaagcaat tccagcacga gcggttcacc
    70801 ctcctgccgg gcgttcacgt gtgcgtccag gacatgatcg agatgacggt ggattacctg
    70861 aaacaacgga tcgtcttcgg aaagcccctg atcacgaagc aggtgctgcg ccaccgcctg
    70921 gccggctggc tgatgaagaa cgaatgcctg aagcagctca cctaccacat cgtccgcatg
    70981 aagatggacg gacgcgacgt cacgcgtgaa atctcgatgg ggaagctgtt tgccgggcag
    71041 ttgatcgccg aggtggccag cggctgcctc cagatgttcg ggggaatggg tttcatgaac
    71101 gaaaccttga tttcgcgtta cttccgggat tcccgcatca tgtcgatcgg cgcgggcgcg
    71161 gacgaggtca tgtatgaaat catcgcccgc ctggagggat tttagcccca tgaccaagac
    71221 cggcaaaaca ggcgacgacg ccgtgccgag gcggtcgatg cggcgtctcc gggaaaagga
    71281 aaaccgcagc aacaccctcc tgaaggcggc tgaaacgctg ttcgggcaga aggggtacca
    71341 ccagacgagc atcgaggaga ttgcggatct ggcggaggtc tcgacggggg ccgtttattt
    71401 ttacttcaag aacaaggagg aactcctgat caagctcatg caggagatcg gacacgatct
    71461 acgcaaggcc ttgggcgaag aatatcggaa gtcggaattt tctctcgata gtttccggaa
    71521 tgtcgccttt gcgttcctgg gggacttctg tctgcggcat cccgagaaga tcgccatctt
    71581 cttccgggaa tcggtcggcc agagcgtgga ggtcgaagaa cagcgcaagc gcgtcttcgt
    71641 caaactgacg tcggatatca aggatgccat catccggatc agccggggac agggcaggaa
    71701 tttcgcgacg gaacggtcgc cggaactgat cgccgtgtgc atcgtcggga tctacgaacg
    71761 aatcgcctgc cattacctgc tctggcagga tcatcccacg gaagtcctgc ggatggcgga
    71821 ggaatcggcc gccttcatgc tgggcggggt gaacagcctc tttgcccccg acgccccgaa
    71881 gaccgccaaa cacgagaaaa aggccccgaa atagcgggcc gagttcccgg ttgtcttcta
    71941 gggcgccgtc gcctccggcg gccgttccgt ccgctcgcgg cggggtacat tccgcgttgc
    72001 ttcaggcgcg ggtgggtcgg agttccatca ggggatgcca gacaccctgc ccgtttttcc
    72061 ggaagatccg gaagtgggcc gttgcatcct ccaggaccac gagcgcgaac gacgccccgg
    72121 tcgtggcgcc gatgtactgg tcgccgggat tgcacaggac gactccgttt tctttttgca
    72181 ggacgggctt gtgggcatgg ccgaacagga ggacatcggc cgcctgcccc ttcgcccaat
    72241 ccgccatctc ctgcagatcc tgctgccaga ccgccgccgg gtggtaggga ttgatgttca
    72301 tgtggtggcc gtgcgccagg aggaagcgcc agccgtccag gtcgagcaca agtttgccgg
    72361 gaagatcgga cccgtagtcc tcgttcccct ggacgccctg aaagggaagg tccgcctcgt
    72421 gcgccaccag gccgccgtcc cggaccccgt ctcccaggtg gaggaccaga tcgagcggac
    72481 cgaacgtccg cacgacgtcc cgcatgtcgt tcttgttgcc gtgggtatcg gaaaagaccg
    72541 cgatgcgcat ggtcatcagg ccggaatgaa acttgccggg tggacctgct tcaggccgat
    72601 ggccgcaacc gggcattcga aggcgcacag gccgcagccg atgcagagcg ccggatcgac
    72661 cgtgacggcg cccgcgccgt tttcggaaat ggcctccacg ggacagacct cgatgcacag
    72721 gccgcaggcc gtgcagctcc cggcgtcgac gtcagcccgg tagcggctgg tcgcaatgac
    72781 gccccgggtc gcggggttct tggcaaaggg gatcccgatg cagcagcaat cacagcagtt
    72841 gcagatgacg ttcccgatgc cagccttgtt ttccgtcatg tggacaagac ccgccgcccg
    72901 ggcctgcttc aggatttcct tgccctccgc gacgtcgatc cggcggccgg tgccgcgctt
    72961 gatggcataa tccgcgcccc ggttgagctg aatgcaggcc tccagcagac ggttgcactt
    73021 cttcatgacg aaccggcagg tgcacttggt gacggccagg ctattggcgt tctccaggat
    73081 cttcgcggca tcctcatagg gtaggacctc gcttttcgac gcgatggcct cgttgaccgg
    73141 gatgacgcgg aaaaaggaag gcaggcccgc ctgggtcacg agggcgggca gagacgggta
    73201 ctcctcctcc gtgaatttca cccagagatc gagcagcgcc tggggggcct ccggccagag
    73261 aatggtggcg tcgtgaaact ggatgatgtg gcggggcatg cggtagaact gccggccgtc
    73321 cttgacataa tcgaatgcga cgccccggtg gtatagctca tcgaggagag cctgcatctc
    73381 cacgaccggc tttccgaacg tctccgccag ctcgtctgcc gttcccggca tggcgttgac
    73441 gatccgggcc tcctcctccg agcagatcat ccgccagaga tcgggaacga tggccgaatg
    73501 cgccataccg accttctgcg ccagttcctg atataggtcc gctgagttca ttctcccctc
    73561 cccttttttt tcattctata cgacaggtcc cgtgaaaatt gcaatggata aacggttcgg
    73621 gtcgcgatga cgacagaagg ttgatattga tcgggtttcg tgttattcag cgacatcaga
    73681 atgatgtaag gggagaagtg cttatggaag acgacaccgg gctggcgctc aatccctggc
    73741 gggaaatcta tccggaaaag ttcgcatcgg aagagcgggt gttcggccgg atccgccgag
    73801 ggaacagaat cttcatttcc acgggctgcg gcgagccgca gtatctggtg cgcgccctga
    73861 tccagtatgt ggaatcgcac ccgacggcgt tcttcgacac ggaggtgctc cacgtctgga
    73921 ccctgggcgt cgccccctac acggatgtga agttcaagga aaact
//
LOCUS       DAOOLJ010000008        72461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_213_length_72461_cov_15.377731, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000008 DAOOLJ010000000
VERSION     DAOOLJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 72461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72461
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213_length_72461_cov_15.377731"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PLW16_02770"
     CDS             <1..1010
                     /locus_tag="PLW16_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69862.1"
                     /translation="TFGQCRASNYLPLLRKALDAAGYEDVPILSFSTLDGQTPSGFGI
                     DRKKLLQKLSLTLVFADALARLYLATVARERSPGEATRVHTAYLERMEEGLAQGRFSV
                     LLRLLREAVAVFNAVPVHDRALPVVGVLGEIFVKHNAFSNHHLVSWLVERGVEVVMPS
                     LAGFFLQHFINEDFNRRAVLRRMGGAERLMLALLNPLIRFRLRQVEAVLEGFRYNRPA
                     HDLRDLARETRRAISLVNQAGEGWLLTAEMIAMISHGVRHIVCLQPFGCLANHITGKG
                     IEKRLRALYPDVNLLFLDMDAGASEINAVNRLHLLLMAAHEAMGTSDGSIPVRRVVVN
                     A"
     gene            complement(1000..1749)
                     /locus_tag="PLW16_02775"
     CDS             complement(1000..1749)
                     /locus_tag="PLW16_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPB69863.1"
                     /translation="MRHIPAVSFRPPPARERRWARWLILLLAGIPLCACAPAPVCTVS
                     TAPGAIGTSGVSPARVLILYQSTYGSTKQYADWIHREIPSVMVDLEACPTTDFGPYDV
                     IVFGSPVRTGRIVVAPFIASSWSAIGGKSVVLFTVSGTPPDHPVLRALYKVSLPADIR
                     KDIRHFPLHGRMVHRTLSARDRALVSVGRAIQKDEVLRKFMQEDFDGVKRENLAPLLK
                     HLRDLLRLFPDCTRSSRPPLRLGQNAARVTH"
     gene            complement(1703..2755)
                     /locus_tag="PLW16_02780"
     CDS             complement(1703..2755)
                     /locus_tag="PLW16_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPB69864.1"
                     /translation="MTEDPTPTPRSWDPALDYDLLQIDPDCSPAELHFAYEKMIAAWD
                     PDRFPHVQSWKETSEKKQSEIREAYDRLRVLHASRETATPEDPSRPEPSPTILSVPVN
                     PGRDDEDPPGGNDIPPRKTVQARLGTAVRRRPGLMAAVAGAGLLLILVLLWPSLYHYE
                     TVRLDGKDYPLRINRLTSRAQYSDGRQWLDPPLPAAALPVTALPAEPLEQAAPPPPPV
                     APFVAAPAAEPPAVATPPVKTVTMPREKPTPGPAIETAPAADRTRAKAVPKGRYGIQV
                     GAYPETAKAEAQANALRAKYPTARVEVIRIEGKGPWYRVLVGRFENRDDALRYLKDHR
                     IGEAYPGSFVQTSTRP"
     gene            3738..5018
                     /locus_tag="PLW16_02785"
     CDS             3738..5018
                     /locus_tag="PLW16_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB69865.1"
                     /translation="MKTVSMRKYILCGLLLMCALIPVDVFCGEPATEATPEIVTKVSA
                     AASDWENSGLVVRKGSKYKISASGRWSLGPICGFTGPDGVGNSAICGGEYIAGSGSLL
                     VGMIGEQGDPFAIGRGIELTAPEEGPLFFKVNGGLKIFCSGYVTVRTSPIAQTPSAPP
                     PLAEKAVSAAKSDIDELPASKAKLNKNAYAIVIGIENYRQKLPRADFAVSDARLVNEY
                     LSKVIGYPEENIVMLTNEHAALGDFVKYFEKWLPNNVEKGGSVFIYYSGHGAPNTKTG
                     DAYLVPYDGDPLFIAETGYSLKRMYEALGKLPARETVVALDSCFSGAGGRSVIAKGAR
                     PLVMNIQANLSLSANITVLSASSGEQISSTYDEKGHGLFTYFLLKGIKDENVIQQDGS
                     IKKEDLFDYIKPQVERIARKQYNNEQTPQLAGRK"
     gene            complement(5098..5349)
                     /locus_tag="PLW16_02790"
     CDS             complement(5098..5349)
                     /locus_tag="PLW16_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB69866.1"
                     /translation="MTRKNHPVDLLLLDIIMDSGIGGLDTYIKAREINLRQKAIIASG
                     FSESEQVHKAPELGAGTYVRKPYVIEKLGMAVRKELDRK"
     gene            5583..6941
                     /locus_tag="PLW16_02795"
     CDS             5583..6941
                     /locus_tag="PLW16_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB69867.1"
                     /translation="MTRFTRRGWAMLAVLAALVGSPVLLWAETYTLQDLCRIALQQSE
                     KIRISEENVAITGLTKDKALSVLFPRLTAYGGYVRFSEDKYTEPSRLFPSGQMIQPNA
                     TSEWGIRLDQSFSLSARELTALRIAKTSFEMTRYTDEAFKNLLLEEVARAYYQVLQAR
                     KIVEIAEANVDRLTLYRQAAEKRLKVGEVTRTVLLRAEGELSGARSDLLQAQNAWEMA
                     RAYLARLVGLPEIEVLREETGLEATPLDPLENFQDKAFRIRADLKAMDRQRQIAADQV
                     RYARGAFWPNVALTAAYARADQEPATATLNRESLYGGVSLNFPFFEGGLRTAELQEAR
                     RRETQAELSYQDLKKTVAIEVQSVYLDLATQRGVVTFLADQLVFARDNYQAVGRQFSF
                     GLANSVDVMDANTLLVSSERKLAEATYSYHLSLLKMDRATGILYQKVMGDRRDASPKA
                     GP"
     gene            6985..8091
                     /locus_tag="PLW16_02800"
     CDS             6985..8091
                     /locus_tag="PLW16_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB69868.1"
                     /translation="MQAYPKRKRTGFTLGLGMAAMLLLPLAGVFGCQNKDEDAKRERV
                     LNVRVRAAEVQTVRPYIEAVGALKPYEEVIVSSEVDGILRRVLVEEGSQISRGTPMAV
                     ISETDYRLEVRRSEAAVRQAEANLANIRQEFERKEALFQEALITRQQYDDVTARRLVA
                     EGEVERSRLALALARERLGKARVHAPLSGSVKEKRVTAGDYIRNGTPLFLIIQTNPLK
                     LLFSVSEKDVGSLRVGQEVRFTVDAFPGRTFPGTVRTVYPHIDERTRTLQVEAVIPNP
                     AQALKPGLFARVTLYSGPAREQVVTPVTSLLYEGQKTKVFLVQGDRSRERFVKVGRKY
                     GEVMEVLEGLEAGEPVVVVGQNNLTEGMKVHVAR"
     gene            8078..11236
                     /locus_tag="PLW16_02805"
     CDS             8078..11236
                     /locus_tag="PLW16_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB69869.1"
                     /translation="MWLADTSIKRPVFATMFIMALVVLGLVSYPSIGVDLFPKVDFPI
                     ATVTTTLKGASPEIMDVDVTDRIEEAVNTVNGVKTITSASQESASRVTIEFVLERDID
                     LAVQDVREKVAAIRSKLPDDIGEPVIEKVDADSSPVLWLNLSGQRSIRDLSTYADEVL
                     KEQLQKINGVGALRMGGLRLRQIRIWLDADRMQAYGIAPSDILTVLKRENVELPGGRI
                     ESLSKEYSVKIRGDIRDASEFNDLVVAYVDGAPVRIRDIGRAEDALDERRSIAKFNGV
                     PCVGIGIQKQSGTNTVEVVERIHAELEKIRKTLPTGMELNVAADQSVFIKRSIAEVQK
                     HIILGSLFAVFAVFLFLGNVRTTLISALALPVSIISTLALIRAFDFTFNNMTLMALSL
                     SVGLLIDDAIIVIENIHRHIEEGMKPREAASFATAEIGLAVMATTFAIVAIFLPVAFM
                     KGIIGRFFLQFALTVVFAVLVSLIVSFTLTPMMASLYLKPKTAVAPVDASDRTRGIRG
                     VWRRMGDAWERGYRWVEELYRRLLGWALRWRKTVLALALVLFVLSLYITKFMGKELHP
                     PEDQGQFIVRLEAPIDYSLGKADEMFREAERIVRGFPEVRAVFYAQGFGAGGVGPVNK
                     AIMFTSLIPKAERDRSQEDIKAVMRTRLNEIPGLKGTAEDVSVIGGGIRNVPIQYVIS
                     GGDLGALQGYAKQITREFARLPGIVDVDTSLEEGKPELKVFIDRDKAADLGVSVAGIA
                     EAVNILISGEVEITRFKDEKKGRRYDVRVQLGSEDRRDPSDLGRIYVRSRDGRLVQLA
                     NVVHVQEGGGPSIIYRVDRQRAIMVYASLEGKPLGQAMEELDAISAKVLPADFTAKYK
                     GAADTMKESFGYLMFALFLGIVMAYMILAAQFESLLHPVTVLLSMPLSFIGAFGALIL
                     TGRSLNIFTFIGLILLMGLVKKNAILLVDYTNTLRERGLSRREAILTAGPVRLRPILM
                     TTFAMIFGMMPVAFALGEGAETRSPMGIAVIGGLVTSLFLTLVVVPAAYDQFDDWRLG
                     LRRRLRRRPSGADAERREEETR"
     gene            11233..11523
                     /locus_tag="PLW16_02810"
     CDS             11233..11523
                     /locus_tag="PLW16_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69870.1"
                     /translation="MKMVLVVYGSEADEEVVGAFKGAGIVRYTKFCEARGVGTETEPK
                     LASRVWPGKNNVLFIGVADEDMPGVLKVVHELKRKHPRAGVKAFVMPVEELA"
     gene            complement(11934..12260)
                     /locus_tag="PLW16_02815"
     CDS             complement(11934..12260)
                     /locus_tag="PLW16_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69871.1"
                     /translation="MCLAFRPPNRYPSGNQAGFDFPLPGAFSDTDATPLLCAGAIGYR
                     SLTLAELTDVEPLGLNGFGASGYLVLKMVRHRYPRTRIRVFARREEERHSALYLGAAG
                     AGDTEP"
     gene            12446..13978
                     /locus_tag="PLW16_02820"
     CDS             12446..13978
                     /locus_tag="PLW16_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPB69872.1"
                     /translation="MDEGGFSEDDLRQIRDEGRAEAEVRRQWETFRRGVRPLTLDRPC
                     RVGDGIETVAAGEIPSLTALYDAAMDAGRIAVFVPASGAATRMFRDWFRVLEDGDFAS
                     HAAGDTFARELPEYAFYPDLLAVLERRGTDVETLLRERDYAAILEAILTSRGLNYGRL
                     PKALLKFHIDAEGGRTALEEHLAEAALYAGDNEGVCRVHFTVSEEHLPAVRDHLRTIQ
                     PRCEVRLGLRFQVTLSTQNPATHTLAVDLEDRPYRAPGGRLVFRPAGHGALLGNLQST
                     GGDLVFIKNIDNIVPDRLKVRIVPYKKLLGGVLLSLQREIFRFQRRLEAGDAGAGERE
                     EMIRFCRDRLHVSLPADFPFRTPMDQTVFLQSRFARPLRVCGVVPNEGEPGGGPFWVR
                     ASDGATSLQIVEAFQVDSASEAQQTRWTEATHFNPVDIACGLRDERGRMYDLDRFVDR
                     QAGCITLKSEAGHPIKALELPGLWNGAMAYWNTVFVEVPLETFNPVKTVTDLLRPQHR
                     QG"
     gene            complement(13998..14981)
                     /locus_tag="PLW16_02825"
     CDS             complement(13998..14981)
                     /locus_tag="PLW16_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HPB69873.1"
                     /translation="MKTRAISLFSGGLDSILAVKILQDQQIDVMGLAFETPFFDAAKA
                     RAAACSIDLPLVVMNITDEHLAMLRAPRYGFGRNMNPCLDCHALMLRIAGDRLKAMNA
                     AFIATGEVLGQRPMSQNKQSLYVVAKHSGYLPLVLRPLSARLLPETELEQRGLVDRTR
                     LLDIEGRSRKRQMALAEHYGITRYPAPAGGCLLTDPMFSKRLRELFAHRPDASAREIE
                     LLKHGRHFRLPGGERVVVGRDQRDNLAIEHLRAEEEGDCFLKMDAFPGPSVLIPRGGG
                     EEACRRAASLCVLYGDAPGGVPVRVQCRRGNQDGTISVTAADRQDAEAWIV"
     gene            complement(14978..16675)
                     /locus_tag="PLW16_02830"
     CDS             complement(14978..16675)
                     /locus_tag="PLW16_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB69874.1"
                     /translation="MMKWARRLLLSVLAVMIIAPSSAPAREDTARLTFTRSASQSRGT
                     VPYRVQPGDTLSNIVARQYGETEYSRKLKYFERLRHLNPSLTNIHQITPGQLVWLFPA
                     EPTGRPGELRADRRLIRARSGDTLYQILRRELHVPEELLPRALEVLKKANPGIQNPDR
                     ILPGQDIYLPAITLDLPINVATNPEIPGESSPSAGLAPPASIPAPPAAAPVLPSPATD
                     RLAALSAVIARLQGSVLTTGWYYVPLPDGGQMTIDCGLFPVVELPDGSVIFLDTLARM
                     PDSFKSLTGKNAGRQSWVRTEPDEPLPAVLKTVLSRSRAFVMEKNLRTISGGDGLRIG
                     LRPDWLIAGRLHAAKGPRMWGLRTDLDPEDLLPAALSHALERSGISIIEVLADGRVAP
                     RPDKPAVAGEVPHLKSTDTLELAHALLAALDFRPQRNTAVRVFSAPEDGFNLSVNADL
                     LVDRNGRTVMFRTKELPAQFIAALRKKDISFTQLKDGAGKTGVVAQVLTGMSIAYTTG
                     LFSLAPPPGARSGQITLHLAGLKIEDGERILYLTEADIDPGLYGCLSAQGGIRIIRF"
     gene            complement(16841..18031)
                     /locus_tag="PLW16_02835"
     CDS             complement(16841..18031)
                     /locus_tag="PLW16_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB69875.1"
                     /translation="MSLSFEFSPEQRMLEENVWRWASTWLEPQMEHLYEKDEMPPDLF
                     HELGKLGLNGIVYEEQYGGAGLGYLETLLVYENIARVSNALSMTVGASQTLAFDNFRR
                     HASDAQKAAILPKACSGESIGCLGITEPNAGSDAMSMTTRAEKKGNTYVINGSKTFIT
                     NAPVADVMVFYAKTDLGKGPHGISTFFCYPKTTKGFVCEKIKKWGMRTSPTGLVTFDD
                     MVLPEENLFGGINQGVKVLTSGLCTERITLSGCALGSMRACLELSLKYAKERVQFGKP
                     IANFQFIQGKLADMYTRYLSSKWMAYSAAAFCDSLEDKSGGKGTDLDRMAAASLLHCG
                     EAGTAVVSDAVQIHGGYGYCQEYAVSRHYLDEKLWHIGAGTSEVRRMIIARELLRDDF
                     ASGR"
     gene            18480..18701
                     /locus_tag="PLW16_02840"
     CDS             18480..18701
                     /locus_tag="PLW16_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69876.1"
                     /translation="MGHAERDVILAREFQQRLEKKMREEELSHLEYWKTQIDSLLVAR
                     PEGVAALQSQIRKVADKMANRIQMLKKGA"
     gene            18701..19279
                     /locus_tag="PLW16_02845"
     CDS             18701..19279
                     /locus_tag="PLW16_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB69877.1"
                     /translation="MQDIANFLYEVGMLQKTPRTGFQFLGSGKESVAEHVLRTIFIGY
                     VLARLDPSVDELKLLRMCLFHDLPEARTGDMNYVNKKYVAVDEAKAIRELTETLSFGG
                     DIAAALEEFNARKTPESLLARDADQLALILQLKEYGDLGNKYSQEWIRFALKRLGTDV
                     GRRVAEAILQTDSSHWWFKDKSDWWINGNHEG"
     gene            19377..20063
                     /locus_tag="PLW16_02850"
     CDS             19377..20063
                     /locus_tag="PLW16_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB69878.1"
                     /translation="MAESHLKLGIAQIQAEQYNSALKELMKANELTPDNARLHYFLGI
                     AYHSKGLRKQAVEEFEKAVALKADYSEAYNFLGTICLSAGQWDRAITLFEKALTNILY
                     DTPGVALYNMGQAYHQKGDYRTALLKYQEAKVREPDTILMPLIEKNMAQSAYELGEYA
                     EAVAHLKRAIELTPELAEAHYWLGRAYVKMGRRDQAVPAFQEAVKCSPASEAGIKSRE
                     HLDALQADRR"
     gene            20084..20710
                     /locus_tag="PLW16_02855"
     CDS             20084..20710
                     /locus_tag="PLW16_02855"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPB69879.1"
                     /translation="MSTKKQRRIVQVQQRKKKQQMKTSLLVAIITVTVGFLILFFVTL
                     FHFVYPPAAGRDAAARSRDKMVCILYFADANERFLVPEKRFIPREDTRAALAEALVKS
                     LIEGSKTGLTATLPEKAGLRKVEIEPDQTLSVRFAKSLPELHPGSTTSELATVYSLTN
                     TLIANIPEIKRVRILAGDEVAASIRGHISLKNPFAFNPEIIAPVSRAD"
     gene            20732..22765
                     /gene="fusA"
                     /locus_tag="PLW16_02860"
     CDS             20732..22765
                     /gene="fusA"
                     /locus_tag="PLW16_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB69880.1"
                     /translation="MAAKTRLSRIRNIGIVAHIDAGKTTVSERIIFYTGRSYKIGEVH
                     NGEAVMDWMPQEQERGITITSAVTTCAWQNHEIHIIDTPGHVDFTIEVERSLRVLDGA
                     AVVFCAVGGVEPQSETVWRQADKYGVPKVAFINKMDRVGADFDLVIAQMHSRFQSIAL
                     PVQIPWGREDQFRGVIDLIRQEAIAWDDSTLGEAYRCVPIPEELQSEARSRRDTLIER
                     LAELDDELAEMYLEGTAVANEDLHRVLRQQTLNLSVVPVLCGSALRNKGIQPLLDAIV
                     RYLPSPEDVPPITGTNPRTGEKEARPSSEKEPLAALAFKIIQDEGRKMTYLRLYSGHL
                     QAGEEVYNASRQKKEKIARLLKMHANKRERIERAAAGDIVAVMGLKDAGTGDTLCDEG
                     HPIILEPIEFYEPVISQAIEARTPADQERMSVGLVKLEEEDPTLKVKYEDETAQMVIS
                     GMGELHLEIIIDRLRREFHAAVNVGKPRVMYRETITESVEQEAVFNRELGEKKHYGQV
                     RLRMVPAERGGGIRIERAETAGAAPEEYLHAVEDGIREAAASGIISGYPVVDIDVTVL
                     QVPIREGDSTTLGYKIAASSAFRDGCGQAKAVLLEPIMSVDVTTPSEFMGEVIGDINS
                     RHGEVQGITPRGPISEILARVPLKGMFGYSTDLRSATQGRAVFSMKFLQFDKA"
     gene            22811..24985
                     /locus_tag="PLW16_02865"
     CDS             22811..24985
                     /locus_tag="PLW16_02865"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB69881.1"
                     /translation="MTKHSEPTGTPSGLSSQPLLDRDKLLQSIFRISTLLTAPVDLDT
                     VLKRILDEVVDAVGFDRGIIRLLDTSKRFLETKVAKNYTPEEEARAFAVPLNLAEHDC
                     VSTKVAKTGAYLAIEDAAVDLRMTETDRMLTRVFHGGSILCAPLKIERNVIGVLAAWR
                     GEKTRFFQEEIDLFLSFASQASIVIHNARLFANHAEKIRELTLLQEAVSEMNFSYDLD
                     NHILNVLISSALKIGKADRVVVYILDIETNRCMIHDGEHQYIEAKEEGDIRLNNTIIQ
                     EALAAKETRIRSVISGGEAGPVMFDGYPHEIAIPLDIQDKFNGALYLAKRTEGFSSDQ
                     INVFDILARNAAVAYDNAIMHSKLSLEAKTLKTEVEKLKEREDRLLGFHNILGTSQKM
                     IGIFHIIREVAAHDTNILVQGESGTGKELMARAIHRQSNRHAKPFVDVNCAAIPGTLL
                     ESELFGYEAGAFTDARKRKIGLLEYASGGTILLDEIGDMSIHVQAKFLRMLEDGHIRR
                     LGGNENIPIDVRFIFATNRDLGRMVTDGIFREDLYYRISVVPVVIPPLREREDDILLL
                     ANYYLDEFNRKFKKRIKGFSLDAERILRAYQWPGNVRELRNIIERVMILQQEGTMIAP
                     EHLPGEIKMAVTKSEPQLRTDILIPRMSPEGIHYDLVLDRVTSEVKRKLFHEALNLTK
                     GNMTAAARLLGISRYKFIREQKKLEQAFEEPLAPGSEQIVRN"
     gene            25366..25575
                     /locus_tag="PLW16_02870"
     CDS             25366..25575
                     /locus_tag="PLW16_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69882.1"
                     /translation="MKPEEKRSGRGSHRLAFPSKPDRATEYEFFISGSADGGGDTPEK
                     KNAEENRGETGATVLGSREDVGKDL"
     gene            complement(25736..26839)
                     /gene="ychF"
                     /locus_tag="PLW16_02875"
     CDS             complement(25736..26839)
                     /gene="ychF"
                     /locus_tag="PLW16_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPB69883.1"
                     /translation="MGFNCGIVGLPNVGKSTIFNALTAAGAEVANYPFCTIDPNIGIV
                     VVPDPRLEALARILSPPKVTPTTMEFVDIAGLVTGASRGEGLGNKFLGHLREVDALIH
                     IIRCFQNPDVVHVVGAVDPLRDREVVNTELILADLDTVERRLEKTERLAKVGDKGARK
                     ATLFLTRLRDVLGRGLPARSLIPDEEHGEDEALRREMHLLTDKPVLYVANVSESMLKA
                     PSPPVEALEQAAAQEGSLVVTICGDMEAEIAELPPEERRGFLDDMGLAEPGLAKLIRA
                     GYDLLNLITFYTTVGPELRAWTIPRGTPAHQAAGRIHTDMERGFIRAEILHYDDFLAA
                     GGIAPAREKGLLRSEGKSYRMMDGDIALFRFNV"
     gene            complement(27147..29576)
                     /locus_tag="PLW16_02880"
     CDS             complement(27147..29576)
                     /locus_tag="PLW16_02880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPB69884.1"
                     /translation="MHKGKRFWSILCSVFIMLLMMTPAALASEADIVLPDLSLVMFGT
                     LNGLLVLKLGLIVCVIGMAFGWMQYRQTKRLPAHQAMLDVSATIWETCKTYVLQQGKF
                     LAGLWILIALCMLYYFGVLSQMDATSIIVILLCSIAGILGSYGVAWFGIRINTVANSR
                     AAFASLSGQPLNIVNICLRSGMSVGLLLVSIELFFMILILAYFPMELAGPSFIGFAIG
                     ESLGASALRICGGIFTKIADIGSDLMKIIFHLPEDDPKNPGVIADCTGDNAGDSVGPT
                     ADGFETYGVTGVALITFLALALADNPVMGGKLIVWIFAMRILMILTSLASYFVNDILS
                     KSLFGDKKQFNFEHPLTNLVWITSIVSISVTFWASHFLLADLPNIGGMPLWLALSIII
                     SCGTIAGALIPEFTKVFTSTSSRHCDEVVNASRQGGASLNILSGLVAGNFSAFWEGLT
                     ILVLMFIAYFVSQTPSVMEIMPPAFKFAAPVFAFGLVAFGFLGMGPVTIAVDSFGPVS
                     DNAQSIYELSMIESRKDAKDVVKQHYGISPDFEMAKHCLESADGAGNTFKATAKPVLI
                     GTAVVGATTMVFGIIILLEKMFGDVIAKLSLVQPEVILGLLMGGCVIYWFTGASIQAV
                     VTGSYRAVVYIKDNIDLNKKEASIKDSKEVVRICTIYAQKGMINIFIVIFFLALGLAF
                     VNPYFFIGYLIGIAFFGLFQAIFMANAGGCWDNAKKIVEVDLKQKNTPLHEASVVGDT
                     VGDPFKDTSSVSMNPIIKFTTLFGLLATEIAVTMTNVGLKTGLAALFFLIAMIFVYRS
                     FYGMRISAEKL"
     gene            complement(29579..30169)
                     /gene="pth"
                     /locus_tag="PLW16_02885"
     CDS             complement(29579..30169)
                     /gene="pth"
                     /locus_tag="PLW16_02885"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPB69885.1"
                     /translation="MHLIIGLGNPGRRYEKTRHNMGFRVIERLSEAHDIPLTDNRFDS
                     VVGKGAMGGAAVLLAKPQTFMNLSGQAVRKLFDYFRIATEDVVVVHDDLDLPFAEIRL
                     KYGGGHGGHKGLISVMQHLGSRDFSRVRMGIDRPTAREDVERYVLEPFAPEEKDRLSM
                     LLETAVEAIGEIVSSGIRAAMGKYNGKAIKNSNEEV"
     gene            complement(30194..30844)
                     /locus_tag="PLW16_02890"
     CDS             complement(30194..30844)
                     /locus_tag="PLW16_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPB69886.1"
                     /translation="METAELKVQIRQEAGKGPARRLRNVGSIPAIFYGQKTQNQMLAV
                     KTAELMKLLRGGDENIFIRLSFDEAGQSREKLVMIKEMQSNPTTGKLRHVDFYEISMD
                     QAFTFDVPLHFSGIPVGVNNGGDLQHLKREIKVSCLPARLPEAISVDISNVEIGDALK
                     IGDLAIPEGLTVLDHADVAVVAVSAKKEAKVVVPEGEEEAVKEPERISDDKKAKEE"
     gene            complement(30857..31798)
                     /locus_tag="PLW16_02895"
     CDS             complement(30857..31798)
                     /locus_tag="PLW16_02895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPB69887.1"
                     /translation="MLERIRLFSGNANRPLAERICQKLGVSLGKAHVTTFSDGETRVE
                     IDENVRGMDVFIIQSTCSPVNVTLMELLIMIDAMKRASADRITAVIPYYGYARQDRKV
                     APRAPISAKLVADLLTTAGANRILSMDLHAGQIQGFFNIPVDNLFATPVLLDYMKRNY
                     SDNVVIVSPDTGGVERARAFGSRLGASLAIIDKRREGPNEAQVMNIVGQVEGKKVIIL
                     DDMIDTAGTVVRAAKALTEQGSLEVSVCCTHPVLSGPAIDRLESSDLKEVIVTDTIPL
                     HERALACQRIKVLSVAVLLSEAVRRIYYNDSVSSLFI"
     gene            complement(31827..31901)
                     /locus_tag="PLW16_02900"
     tRNA            complement(31827..31901)
                     /locus_tag="PLW16_02900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31867..31869),aa:Gln,seq:ttg)
     gene            complement(31941..32777)
                     /gene="ispE"
                     /locus_tag="PLW16_02905"
     CDS             complement(31941..32777)
                     /gene="ispE"
                     /locus_tag="PLW16_02905"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPB69888.1"
                     /translation="MITRLSPAKVNLHLKVLRKRPDGYHDLATLMQRISLYDEMSFAR
                     RSEGVSLHCPDGTLPEDGNNIVWRAAAALLTHAPDPGGVDITLKKRIPLAAGLGGGSS
                     NAATTLLAVNELIGSPCNLQELLHVGATLGADVPFFIFGRTAWAFGIGDRLKVAPEVP
                     RFWFVLVNPGFALSTKIIYEKLNFRLTNRSINYSIPRFCEAGDWAAGLYNDLEKVSIR
                     LHPELADIKGYLLDLGAIGALMSGSGPTVFGLFATEDGAETAEVALKRFSPYSVFVAR
                     TL"
     gene            complement(32774..33235)
                     /locus_tag="PLW16_02910"
     CDS             complement(32774..33235)
                     /locus_tag="PLW16_02910"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPB69889.1"
                     /translation="MEEEKKETGFVIKDRRHFDESGEVRTEEAKTMPPPAAETGAVPP
                     DTASEAPGRTDAAPASPQDAPGEEQTYPAIDFQNFILSLSTTAMYHFGDFPDPTTGET
                     RRNLSAAKQTIDILGMLRDKTEGNLKDAEKQLLEGLLFELRMRYVKEKRTR"
     gene            complement(33312..34160)
                     /gene="htpX"
                     /locus_tag="PLW16_02915"
     CDS             complement(33312..34160)
                     /gene="htpX"
                     /locus_tag="PLW16_02915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPB69890.1"
                     /translation="MNSFKTALLLGALTGLLLLIGGYFGGRTGLVIAFAFAMIMNFGS
                     YWFSDKIVLKMYRAQPVTEAEAPELYRVVRDLSMKASLPMPRVCIIPQDTPNAFATGR
                     NEKHAVVAVTEGILRILSRDELEGVLAHELTHIKNRDILVGSIAATLAGAIVMIANMA
                     QWAAIFGGVSRDDDDSGGGMIGLILTAILAPIAATIIQMAISRSREYMADEGGARISG
                     KPYGLAGALEKLSRASQALPMNAKPATAHMFIVNPLTGRPLMNLFSTHPPIEERIARL
                     RSMRPM"
     gene            complement(34248..36827)
                     /gene="clpB"
                     /locus_tag="PLW16_02920"
     CDS             complement(34248..36827)
                     /gene="clpB"
                     /locus_tag="PLW16_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPB69891.1"
                     /translation="MRFDKFTLKVQEALQEAQGLASTYGHQAIDAEHLMRACLAQPDG
                     IVGAILKKLGADPAAVEAECRQALEKLPRVTGDAGAQVYLSPRLNRIMDRALTEAARL
                     KDEYVSAEHLLLAMTEEKDGAIPAILKRSGVTKDRIFQVLVDIRGSQRITDPNPEEKY
                     EALKRYGRDFNEIARKGAFDPVIGRDEEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPDSLKNKRVVGLDIGALIAGAKYRGEFEDRLKAVLKEVTAAAGEII
                     LFIDEIHTVVGAGAAEGSMDASNMLKPALARGELRCVGATTLNEYRKHIEKDPALERR
                     FQPILVKEPTVEDTIAILRGLKERYEVHHGVRIKDTAIIAAATLSHRYISDRFLPDKA
                     VDLVDEAASRLRIELDSLPAEIDVLERRTIQLEIERQSLKDEKDPTTLERREKIDREL
                     ADIRDDMDRMKRHWSAEKESIKKIQTLKEEMEALKTDELNAQREGNLARAAEIRYGKR
                     VELDRRLEAEQFALADVQKDQKMLKEEVDEEDIAEVVANWTGIPVTRMMESDVQKLIH
                     MEKRLRKRVIGQDEGIAAVSSALRRARAGLQDPNRPIGSFIFMGPTGVGKTELARALA
                     EFMFDSDQAMIRIDMSEFMEKHSVSRLIGAPPGYVGYDEGGYLTEAVRRRPYTVVLFD
                     EIEKAHADVFNILLQILDDGRLTDGHGRTVDFKNTIIIMTSNIGSQWLQDPSGDEPAR
                     RERMMEALRAAFKPEFLNRIDDIVIFRSLTLADIDRIIDIQMGLIEKRLAERKLSIEL
                     TETAKNYIAEAGYSPVYGARPLKRVLQKLVLDRLAMDLLESSWREGDRITVDVEGEKI
                     IFK"
     gene            37323..38183
                     /gene="rpoH"
                     /locus_tag="PLW16_02925"
     CDS             37323..38183
                     /gene="rpoH"
                     /locus_tag="PLW16_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HPB69892.1"
                     /translation="MDLPAVTSTLDLYIAEINQFSLLTPEEEFRLAVRLKKYSDMEAA
                     ELLVVSNLRFVVKIAHEYRNYGLKLADLIQEGNIGLMHAVKKFDPYRGYRLISYAVWW
                     IRAYMQNFIIKNWSLVKIGTTQAQRKLFFKMGQAKKQLDALSEKHPEFSEIAQSLGVK
                     EIELAEMDQRMSHRDVSLDAYVSDEGDAAHIDFLTDDGENQEMALIRKEEMQLVQRNI
                     AGAMANLNEKESYIVKYRIMADHPQTLQEIGNRYSITRERARQIEKQALKKLRLALPY
                     LGERTELIEG"
     gene            complement(38250..39599)
                     /gene="dnaB"
                     /locus_tag="PLW16_02930"
     CDS             complement(38250..39599)
                     /gene="dnaB"
                     /locus_tag="PLW16_02930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPB69893.1"
                     /translation="MSEMDPTLHKLPPQNLEAEQSVLGGILLENHALNAVLEILDAKD
                     FYSEAHRRIFAAILALSERSDPIDLITLSNCLKDRKQLDSVGGAVYLASLVDNVPSAA
                     NVAYYAKIIKNKAILRGLIGTATEIITNSFQTGADVDQVLDEAEHAIFEISENKIRPS
                     FYPFRDIIKKSIETVEHLYENKEMVTGVPTGYERIDDLTSGFQKSDLIIIAGRPSMGK
                     TAFALNIASHAALESGIPVAIFSLEMAKEQLALRMLASEAKVDSQRIRKGFLGETDWE
                     KLVGAAGRLSEAPIFIDDTPAITVLEMKAKARRLKAENGLELILLDYLQLMRGGAYRE
                     SREQEISEISRSLKALAKELNVPVVALSQLNRKVEDRTNRRPQMADLRESGAIEQDAD
                     VIAFIYRDEVYNKSEDNPERGIAEIIIGKQRNGPTGVVKLAFLEKYTCFENLASGTE"
     gene            complement(39605..40051)
                     /gene="rplI"
                     /locus_tag="PLW16_02935"
     CDS             complement(39605..40051)
                     /gene="rplI"
                     /locus_tag="PLW16_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPB69894.1"
                     /translation="MKVILKDNVASLGNLGDTVKVSDGYARNFLIPKGLAVEANEKTA
                     KVFAEQKKHIFQQAEKIRKGAEATAASLQGLTLTIARRVGEQDKLFGSVNTKDIEGAL
                     QERGLAVDRKAIQMEEPIKAVGEYDVKIKLPAGIVAEIKVCVVAEA"
     gene            complement(40088..41059)
                     /locus_tag="PLW16_02940"
     CDS             complement(40088..41059)
                     /locus_tag="PLW16_02940"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HPB69895.1"
                     /translation="MNDRTGLRSHLDLGIGIAVSTLFFVGVALIPAGGTGVIVFLPLP
                     TLLFYAKLGRWRGVVVCFVSLAATTFILLASSRPLNLIPVLLSASLTGLVLGELTVRG
                     VDFVRTLAIGSAVLAGLSLFLLLAESLRTGSAPWHIVATHLSDSLRENLKVYSELDLP
                     PEQVAIIRDNAPQFQRFLMAIFPSVAAAGIVLSVWANLLAARFLLRRGKASALGNPDL
                     SGWKAPEGLIWLLIIGGGLALVPHAGAKIAGVNVLILCGFVYLLQGLAITGFLFRMKR
                     VPAWLRFFFYALLVLQQYLLLIVAAFGMFDLWVDFRRFIKPRDNPSL"
     gene            complement(41086..41355)
                     /gene="rpsR"
                     /locus_tag="PLW16_02945"
     CDS             complement(41086..41355)
                     /gene="rpsR"
                     /locus_tag="PLW16_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPB69896.1"
                     /translation="MAVPERRDQRPPRPQRRFFHRRKFCRFCTDSALIIDYKSPDVLR
                     DFVTERGKIMPRRITGNCAKHQRELTTAIKRSRSIALMPFAVTEG"
     gene            complement(41357..41809)
                     /gene="rpsF"
                     /locus_tag="PLW16_02950"
     CDS             complement(41357..41809)
                     /gene="rpsF"
                     /locus_tag="PLW16_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB69897.1"
                     /translation="MRRYDIIFIVPVDLPEDEISAMCERYGTFITDHQGIVVKIDRWG
                     KRKLAYEIKKQRRGYYLLFDVVSDHAAITEIERNFKIDERILKYLTVRRDGTFTREAI
                     EKEIADAAKKAEEGDAGKQPAEPIAPKMVIPAAAAQETAAKTVNEGVK"
     gene            complement(42351..43901)
                     /locus_tag="PLW16_02955"
     CDS             complement(42351..43901)
                     /locus_tag="PLW16_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPB69898.1"
                     /translation="MSEASKRAASVSRRQFMKTVGGAAAVAGMAVGGVGLGGQALAAP
                     GKMPKKWDETYDVIVIGSGFAGLTAAIEARNGGALVTVIEKMVVPGGNSIINGGDFAA
                     AGNKLQKEQGVQDSAELMLKDMLKAGSYLNHPELAKLVADGSNAALEWCQQYVGAQFA
                     RLNFHGGHSVKRAVQTVNASGSELVNKMLAKAKELGIGVQTRTKLVRLVLNDAGRIVG
                     IEVRKGYRFPNEASGKAAFLKARRAVVLGSGGFSQDVKLRQVHDPRLNDKFDSTNQPG
                     ATGEALLAACQVGAMDVQMDWIQLGPWTSPDEKGFGLVPLFCERLVGYAPMVDPATGK
                     RFIKETGDRKVRADAIILLGHPVLIFGDAYAVPKQVFSNALEKGLASGAIRKFDSLET
                     LAQHYNIPRAALLEEVARWNGYVEKKKDPQFDCMIFPDAKPTVMPPFYAARLWPRVHH
                     TMGGLVIDTNAQVTGFNMKPVPGLYAAGEVTGGVHGAVRLGSVAMADCVVFGRIAGRN
                     AAAEKPWS"
     gene            complement(44088..45056)
                     /locus_tag="PLW16_02960"
     CDS             complement(44088..45056)
                     /locus_tag="PLW16_02960"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HPB69899.1"
                     /translation="MLKDVIDPDLLNHFEAGLDPRNPHLSAISARIIGYGEMSTIFVI
                     DHPGQAHIAYKRMPIFRTNAEMDAYERLFEAYNTGLGRVGLQVPEWASARVVPKRGNR
                     IVYTAQVRLPSASIGNALLHTLAEGSIGLLFGCVLRELAKVFASHRVHPSLIFGIDGQ
                     ISNWALKHGRSDGPITEASELWYLDTGTPLIRRDGVEQLDPELFLRCTPSFLVWLIRL
                     LFLEDVMTRYYDFRKVTIDLIANVYKEQRPELIPLLLETANGFFAGKGAAFGIAPLTA
                     EEVSAYYRQDAMIWRVYLAFRKIDRLLHRHVLREPYVYILPEAIRR"
     gene            complement(45050..46309)
                     /locus_tag="PLW16_02965"
     CDS             complement(45050..46309)
                     /locus_tag="PLW16_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB69900.1"
                     /translation="MKHIGQDSKEDIVARYSLHVSSGKAEFFKNVGIDFVFGRREGPY
                     VWDAAGEKRLINCHCNGGVFNLGHRHPRVIRTLIAALEELDIGNHHLLSEQRARLAER
                     LASLTPGDLSYTVFGVGGGEAVDLALKLARGATGRTKIVSAAGGYHGHTGLALATGDE
                     AYRAPFGPNLPGFMQVPFDDPEALAGALDATTAAVIFETIPATCGMPVPDLGFFRTAK
                     ALCERVGALLIIDEVQTGLGRTGKLWGIEHFDVVPDILVIGKGLSGGVYPISATCLRA
                     DLQSFFHENPFIHVSTFGGAEVGCPVALEVLEITSAPPFLEHVRELAGFFRTGFESLR
                     ARHPELVVGLRQLGLMMGVEMVNDLCGPVLTKVAYDHGLLCIYANNNRKVTQLLPPLT
                     IDRAVAQEILERLDLALAETKRVLAIC"
     gene            complement(46725..48227)
                     /locus_tag="PLW16_02970"
     CDS             complement(46725..48227)
                     /locus_tag="PLW16_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HPB69901.1"
                     /translation="MSILLAERAGSFGDRTAIIAPEGTFTYRQLLDASARVASFLLNG
                     SLDLEERPVAFLAPPGFQHVAVQWGIWRAGGIAVPLSLSHPRPELDYVIGDASPVAVI
                     AHPDVSDPLLAVARDRGLRFAHGTDILEHPLEALPPVVTSRQAMILYTSGTTGRPKGV
                     VISHDTLAAQITSLIRAWEWTENDRILEFLPLHHIHGIVNVVSCALWAGAVCEILPAF
                     NAWKVWDRIRRGRLTLFMAVPTIYVKLIDAWVRSSPADRKDMSDGCRKMRLMVSGSAA
                     LPVSTLEKWREVSGHVLLERYGMTEIGMALSNPLHGKRLPGCVGVPLPGVEVRLTDEA
                     GAAVAPDIPGEIAVRGRTVFSGYWRRPEETRKAFRSGWFMTGDVAVCENGVYRILGRS
                     QVDIIKTGGEKISALEIEEALRTHPDIRECAVVGVPDPVWGERVCAAVVLEAGRCLDT
                     DSLRVWLKQRLASYKVPRETVSLEALLRNAMGKPIKPAVKMIFQHPDGRL"
     gene            complement(48456..48532)
                     /locus_tag="PLW16_02975"
     tRNA            complement(48456..48532)
                     /locus_tag="PLW16_02975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48496..48498),aa:Arg,seq:ccg)
     gene            48661..50880
                     /locus_tag="PLW16_02980"
     CDS             48661..50880
                     /locus_tag="PLW16_02980"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPB69902.1"
                     /translation="MKAFNPIGNGVGLIALVLLLLFPADAAALNEIINIRHWVAPDHT
                     RIVIDTRDEPQYTVKELPEKIILEFPSMAPPEAKKKWIQILNKPGIKKILLSIRPQQV
                     IRVELVLAAHTETTVFPLKAIEDKPYRVVVDIELPEAAKEATQAREQVRVLKKKRIVV
                     IDPGHGGEAPGAVGKHKTLEKDVVLAISKKLRDTLNRRKGYHAYLTRTGDYYVPFKKR
                     MRIAKEYGADLFISVHADAEKSRQAQGISVYCLSMGGASNEAARLLAKKENLADVIGG
                     VANGNGNDESDPIILDMFQTHTINRSRAFGVATLQSVRAVGPLKFDTVQQAPFLVLKS
                     PEIPSILVETGYISNPNEERLLKKPSYQRRMAEALARAIVKVLSSSGEGVPTEVASST
                     VGANDDQSASKGTDASIGPPTSDKAPTERIEKGPLAATGTLDPSGASTTAALPQPRRE
                     GEAKARTKTEPKAEAKAFRRHIYVVRRGDTLTAIAGRHGVTLAQLKEANDWSRGQRLL
                     AGQKLILPGRSAESDVVPAPQAAARSERKGKAASVYKVRKGEGLEQIARRFGTDVDTL
                     CRLNNLRRDRPLYVDRLLVLPQPRREGEAKARTKTEPKAEAKAFRRHIYVVRRGDTLT
                     AIAGRHGVTLAQLKEANDWSRGQRLLAGQKLILPGRSAESDVVPAPQAAARSERKGKA
                     ASVYKVRKGEGLEQIARRFGTDVDTLCRLNNLRRDRPLYVDRLLVLPPASGVIRGDR"
     gene            50877..51320
                     /locus_tag="PLW16_02985"
     CDS             50877..51320
                     /locus_tag="PLW16_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69903.1"
                     /translation="MKPVPFILVVAVFWLFAAPTVAQMEPFRVKAELAPSAPRLVHQG
                     KIVGRKLVQPPADEAGPGIGTGVEMPADDGPLFRFRLSDSISVYLSQYEGLTTGRNLT
                     QPFGETNTMSVWKILPSLWQQGATRDTLSTAAGMFEPEIGLGIAF"
     gene            51409..52050
                     /locus_tag="PLW16_02990"
     CDS             51409..52050
                     /locus_tag="PLW16_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69904.1"
                     /translation="MDVCKGLHAFIWQDYRENNCNTFLIDGEKKILIDPGHRHLFQHV
                     ERNLASLKLTPADIDVVIVTHGHPDHLEAAELFSKPTLVTMNEAEYRLVKSWSGSYFE
                     GPDPDFFLQEGDLRIGAEDFEIIHTPGHSPGSICLYWPARKALFAGDVVFREGIGRTD
                     LPGGNGSQLKASIKKIAVLEVEYLLTGHGDIVVGREAVRENFRAIEAYWFQYL"
     gene            complement(52045..52563)
                     /locus_tag="PLW16_02995"
     CDS             complement(52045..52563)
                     /locus_tag="PLW16_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69905.1"
                     /translation="MAEIKSTLDLIMERTKHMQLSPEEKAEIRLRETSGTVRGWLQKF
                     ADGLLSPETLESLYAEASGKSPEVRNLMRAALIERISLDGENMETLALIERLLDVPAS
                     PLKDLVRSFRNKRKRLDRQGRDAARAALADYGITGGAVVPNPSLDPARPDALAKLEAA
                     FLKKREALSARL"
     gene            52650..53639
                     /locus_tag="PLW16_03000"
     CDS             52650..53639
                     /locus_tag="PLW16_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB69906.1"
                     /translation="MRTAWRLAVLFGLGILALPFHPGVATAEVTTLKLAHVVSVDGAY
                     HLGAQEFGRLVALKTQGQILIDIYPSGRKGDGEREILSAMQKGKIDLVVISTGPVGAI
                     VPRMRVCDLPYLFRNTAHADRVLDGRIGAMLMEDLRGAGIRGIAFWENGFRHLTNSRR
                     AVVTPEDVRGLTIRTMENEIHQAFWRQLGAKAVPMPWGKVYRALKNKTVDGQENPIAI
                     VRTEKIYKVNRYLTLTRHAYSPALLIMDAKKFDGLGAEQQRAILEAGREAAWWERRFV
                     REKEAAMLGELEAAGMVLVTPDRLPFKQAAESVYKTCEDVAVSDLVRRIRRVK"
     gene            53656..56325
                     /locus_tag="PLW16_03005"
     CDS             53656..56325
                     /locus_tag="PLW16_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HPB69907.1"
                     /translation="MGDLARLLSPEVIAVIGASEREGTLGRRILENLLGATGRRIYPV
                     NPGKERTLGRDCCPTVRDIPEPVDMAVIATPAPLVPAIVKECGQAGVQGLVIISAGFR
                     ESGPDGERREDEIRALQRRFGMRIIGPNCLGVIRPGIELNASFLKDTPPPGKLAFLAH
                     AHGFGRSLLDWALSAHVGFSTVVSLGSAIDVDLGDLIDYLGNDAQTRSIILYLEDQLG
                     QPRKLVSAIRGFARTKPIIILKPPLLEIGAENGRSHTAILADPDRVCDAVIRRTGAVR
                     VKEAQDLFNAAAVLCSAYQPKGPRLAIMGNARGVGLMAANRLLRSGGQLALLTPETQR
                     GLAGVLPAHVSRDNPLPLQRNADVGRYEQALSLLQADPQVDGVLVIYTPQEAARDEEL
                     AEVLVPLAQSVRKPVITTWLGGQGVRLGRDRMGKAGVPAYETAEEAVRTYLYMYEYER
                     NLELLHETPSELSIDDAPPKHHLKTLIRNVRRKGVSVLTEDDAMKFIRNYGIPLVDTQ
                     LVASPEEAVEIARRVGYPVVLKIVSPDVIYRQDVGGVVTGVDSDEKLRKEYGKLLQRF
                     RARAPHAEIRGIAVQKMVELIDYEIILGARRDPTFGAIILFGMGGVGVEIFRDFSLGL
                     PPLNQSLARRLMEDTNVYRMLQGYRGKTPADLRQIEGLIVSFSNLVVDFPEIGEIDIN
                     PVAVSDGIARVLDARIILTDEPLPDAGAPYPHLVITPYPTRYVANWSLSDRTPIVLRP
                     IRPEDEPLEQDMFQTLSEESLRGRFYQTIRTISHDLHVKFCNIDYEREMSIVAELREG
                     ERRRLIGIGSLISETVAARGEFAILVHDDFQGKGLAYKLLDTLVGIAVEKGLKEFYGI
                     IERRNRKMVSLCRKMGMVEEPLPDGLIRVRLVL"
     gene            complement(56329..56790)
                     /locus_tag="PLW16_03010"
     CDS             complement(56329..56790)
                     /locus_tag="PLW16_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69908.1"
                     /translation="MASRFKGLAWLLSSLVIVTGCLSFRVEKVTIGPDVPSAAAGLTE
                     GRTTLAEILAQCGAPLVVEDLGGETLLIYEKKFYRGGQITLGIPMSDVTGTNINVNTY
                     GRLWRYDTVGMILDREMTLRRLFSIRGSATPLWRSFWEDSAPAQTEAPPPQ"
     gene            complement(56774..57181)
                     /locus_tag="PLW16_03015"
     CDS             complement(56774..57181)
                     /locus_tag="PLW16_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69909.1"
                     /translation="MENMQDSGRWRNAVGMLGLGVLCLVLAGCAVFGRDKNFHPYDPA
                     ALTQIVPGKSTADDVTRLFGPPTQVVKLTGGNAYVYSRSLSKGTGLWLVFVTLVNYDT
                     QYDQVVFFLNPQNVVTHCGSALRSGDAAYGLPF"
     gene            complement(57197..57874)
                     /locus_tag="PLW16_03020"
     CDS             complement(57197..57874)
                     /locus_tag="PLW16_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HPB69910.1"
                     /translation="MKRPAGPPLLLACILLIALCLNLPPRLPRNIGIVAADIYRWTDV
                     DGVTHLSDSPPAAASSPPNAVTVRTYSEPQREPEGAPAPSLDEHRGEAVIPFTKNSGG
                     IIVVDALINRSVPARLVVDTGASVITITEALARRLSHSALSSPEAVIIEGIGGTLQGR
                     SILIPRLDVGNVGRDHVPAVVMSGDNGFRGPFDGLLGMNFLGEFELAVDYENNWIVLK
                     EKNRSSR"
     gene            complement(58141..59379)
                     /locus_tag="PLW16_03025"
     CDS             complement(58141..59379)
                     /locus_tag="PLW16_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPB69911.1"
                     /translation="MSEKNLESSRWWIAIAAIVMQLSLGTVYAWSVFKKPLMTTHGWG
                     ETETQVTFMICIFVIGLSAAFGGTLVDKKGPKFVATIGGILFGVGTLLAGLADQIGSI
                     WLLYLGFGLIAGLGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGLGAFFMGKIAPA
                     MVISMGVANTFYIWGLIFLVLVTGAAQAYKNPPQGWLPAGFKPAASSGVTAADSFTFE
                     RAAKTPQWWMLWLMLFLNITAGLGLISQLSPLAQDVIKKTLADISAAELALAGGTILA
                     IASIFNGLGRLLWAWISDAIGRKNVFIIMFITQAVLYILLPQIGDKLIFTIIACYLLA
                     CYGGGFATMPAFAADSFGPAYIGKVYGMMLTAWGLAGVVGPLVFAQLKGIALYVAAGL
                     LIVGFLLAVAYKKPAVVAKA"
     gene            complement(59431..62127)
                     /gene="fdhF"
                     /locus_tag="PLW16_03030"
     CDS             complement(59431..62127)
                     /gene="fdhF"
                     /locus_tag="PLW16_03030"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(61060..61062),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPB69912.1"
                     /translation="MSSVPLNIDGRDLIGKPGQTILEVARENGIFIPTLCHLKDTTNV
                     GACRVCVVEVENARSLVASCCMPISSGMVVRTDTPKVRASQRMIVELLWSSGDHNCLT
                     CEQNGACELQDLVYWLKIDHPRFAIQSPGHPVETTNTMIQRDLNKCVLCGRCIRACNE
                     IQVNEVLDFSMRGSHSKVGPAFDADYIDSECVFCGECVNVCPTGAITFKQARFSGRPW
                     EMKKVRTTCAYCGVGCQIELNVHDGRVIKVTGNREFGQPNQGSLCVKGRFGMDFIHHP
                     SRLTTPLIRKKKGGELTEATWDEALDLVSKKFEALKKQHGPDSIAGLCSARCTNEENY
                     LFQKFMRAVVGTNSVDHCARLUHSVTVAGLAAAFGSGAMTNSIEEIEHTEMILAIGTN
                     TTENHPVIGAKAKRAVRRHGAKLLVIDPREISLTKYADLWLRQKPGTDVAVLNGLMNV
                     IVAEKLYATDYVTERTEGFEALKKVVKKYTPAYVEDITGIPAEDLKKAARLYAGAGRA
                     SILYAMGLTQHTTGTDNVKSVANLAMLCGNIGIEGGGVNPLRGQNNVQGACDMGGLPV
                     SLPAYQPVANDEARGRFEKAWGVTLPPRPGLTVVEIMNAAHKGDVRGLYIMGENPMLS
                     DPDLTHVEAALKHLDFLVVQDIFLNETAQLADVVLPSAAFAEKDGTFTNTERRIQRIR
                     KAVDPPGQALADWEIIAGIADRMGYPMSYRDAEEIMAEIAQVTPSYAGITYERIDREG
                     LQWPCPTQDHPGTRFLHKEKFTRGLGLFHAVEYIPPAELPDREYPLILSTGRVLYHYH
                     TGTMTRLAEGPSERCPESLIEIHPQDAKAYGITDGQRVRVTSRRGQVEAKAQVTEKSP
                     AGTIFMNFHFREVAVNLLTNAALDPIGKIPEYKVCAVKVEAA"
     gene            complement(62224..65280)
                     /gene="nuoF"
                     /locus_tag="PLW16_03035"
     CDS             complement(62224..65280)
                     /gene="nuoF"
                     /locus_tag="PLW16_03035"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPB69913.1"
                     /translation="MKVFRSHLLLCGGTGCHASGSLEVKKALLAELDKRGLADEIKVV
                     ETGCNGFCAMGPIMVVYPDGVMYTLIQAADIPELVEEHLVKGRVLERLLYKEPATGET
                     IPTMQEIPFFALQELRVLRNRGIIDPEKIEEYIARDGYAGMAKALTEMSSEDIVKEIL
                     DSGLRGRGGAGFPTGLKWKFASQSPGDIKYVLCNADEGDPGAFMDRSVLEADPHAVLE
                     GMAIAAKAIGAHHGYIYCRAEYPLAIHRLNIAITQAKEAGLLGKDILGTGFDFDLEIY
                     QGAGAFVCGEETALMTSIEGKRGMPRPRPPFPAVAGLWQRPSVLNNVETLANIGQIIL
                     RGAAWHASVGSEKSKGTKVFALTGDVNNVGLVEVPMGTPLGKIVFDIGGGIPKGKKFK
                     AAQLGGPSGGMIPTQHLNAPVDYEKVAELGAIMGSGGLIVMNEDSCAVDMARFFMDFC
                     QDESCGKCTPCREGTKRMLEILTNITQGQGKEGDIPLLEEMAGIIKDSALCGLGQTAP
                     NPVLSTLRYFRHEYEDHIKNHHCAAAVCSALFKSPCQHTCPIEMDIPAYIALVRADRF
                     EDAYRVLLKTNPFPSVCGRVCDHKCQSKCRRGKMDEPVAIKFLKRFITDNAPRPKITA
                     VPVDRKQKIAVVGAGPAGLTAARDLALRGYKVNVFEELPEAGGMLRWAIPAYRLPRNI
                     LAREIDDVRALGVDIQCNTRVGKDLPWRKVAAEHDVIYLAPGAHASQKMDIPGEDLGG
                     VWGGVEFLRDFNANEEAWLSGKKTLGAKVAVVGGGNSAIDAARVAARLGAEVTILYRR
                     ERKDMPAAEEEILAAVHEGIKIEYLVAPLKIQGKDGKVSAIACQRMMLGEFDRSGRKR
                     PIPVEGSEFTLSVDAIVAAIGQVPDTSFVPEDSGVSVNRWACLDLVPDSKSQTTNPKF
                     HAGGDAVTGPDTVIAAIAAGHQAARDIDASIRQSAGEPAWEAPEEEKIDIPLVIDEET
                     LESPQTAMPEMAPSERRQGFAEVELGFTREAAIAEACRCLRCDIEI"
     gene            complement(65350..65733)
                     /locus_tag="PLW16_03040"
     CDS             complement(65350..65733)
                     /locus_tag="PLW16_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPB69914.1"
                     /translation="MAKLKISDLKKIKEKVHAETALREGDRRVRVTVHMGTCGIASGA
                     REVLDALMREIEESGVSDVVITTSGCMGLCSREPLVTVEIIGREPIKYEYVNPNKMRQ
                     IFKRHVLEGEVQTPFVLARGAEIVK"
     gene            complement(65756..66250)
                     /locus_tag="PLW16_03045"
     CDS             complement(65756..66250)
                     /locus_tag="PLW16_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB69915.1"
                     /translation="MKAELDDLSKEFTPEQIAQLDAIIAKHKGKPGGLIPVLEEAQVT
                     LEYLPPSVQARIARGLNLPFSRVYGVVTFYSFFTMTPRGRHTVRVCLGTACYVRGGKA
                     IAETLEKTFGIKEGETTPDRRFTYETVRCLGACGLGPVVVVDDDVHGRVKPAKVKDIL
                     GPYV"
     gene            complement(66286..66834)
                     /locus_tag="PLW16_03050"
     CDS             complement(66286..66834)
                     /locus_tag="PLW16_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB69916.1"
                     /translation="MLELSHHILDIAENATRAGSKTVRITLTEDTHSDRLTLEVADDG
                     RGMSAEERGRALDPFFTTKKVRRVGLGLPMLADAAQRTGGTFTLDSAPGRGTRLEASF
                     GLCHLDRQPLGDVAASLVSLIAGNPDVDFVYRHRHDAAEYVLDTREIRQELDGVPMSH
                     RDVLKLIRDNIVEGLEEIGSEG"
     gene            complement(66839..67576)
                     /locus_tag="PLW16_03055"
     CDS             complement(66839..67576)
                     /locus_tag="PLW16_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HPB69917.1"
                     /translation="MLKEFRCDLHIHTCLSPCADLDLYPSALVGKALKTDLDVIAVCD
                     HNSSENVPYVLRCAAGTSVVVLPGMEVTSREEVHVLALFDTLDPLARFQVDVYAHLSG
                     SNREEVFGCQAIVNDVDEVEGFNDRLLIGATDIPLDGLISLIHHHGGLAVASHIDREA
                     FGVLGQLGFIDSSLAFDALEVTFRLGIREARRRYPEFGAYAFVTSSDAHFLQDVGQGM
                     TRIRMAAPTLAELKLAFQRRDGREILE"
     gene            complement(67641..68012)
                     /locus_tag="PLW16_03060"
     CDS             complement(67641..68012)
                     /locus_tag="PLW16_03060"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPB69918.1"
                     /translation="MTETSPIDLSTLVRLLQLQVRTGADRLDRPVRGGYVGDLLSDVM
                     GNSHEGDLWITRQVHPNIVAVASLKDLAAIVLVNGSEPAPETLTKAEAEGIPVLLAEE
                     SAFAVAGKVYALITGAVQIAT"
     gene            complement(68023..68478)
                     /locus_tag="PLW16_03065"
     CDS             complement(68023..68478)
                     /locus_tag="PLW16_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB69919.1"
                     /translation="MVPCTAMADEPLIYENQFNVQGGNFNDAGKVASHIKAILKGFGI
                     PSEAVRKTALVAYESEINIVSYARKGVIHLRLARDQVVIETIDEGLGIADIELAMQEG
                     YSTATDKIREMGFGAGMGLANIRNFSDIFQISSEPGKGTSLKMIIKWRP"
     gene            complement(68453..68830)
                     /locus_tag="PLW16_03070"
     CDS             complement(68453..68830)
                     /locus_tag="PLW16_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPB69920.1"
                     /translation="MILLEIKDLLDAEVLVGENQMDMEVKTAFGADLMSDVLAFAQSG
                     SLLLTGLTNPQVIRTADVLDIAAIIIVRGKRPSPDTVQLAGDLGIPILVTRFILFETA
                     GRLYQAGIRGCLVKVDGSVHGHG"
     gene            69028..69915
                     /gene="xerD"
                     /locus_tag="PLW16_03075"
     CDS             69028..69915
                     /gene="xerD"
                     /locus_tag="PLW16_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPB69921.1"
                     /translation="MLPQIEDYLNYLAIEKGASRHTLAAYRHDLGRYRVFLSERNGGD
                     GLTIEPERLLDYLTDLKRSGLAASSINRGFAAIRGFYRYLLRERIITENPVAHIERAK
                     TGLRLPDVLSRQEMELLLARPGTNGPAALRDTAILELMYATGVRVTELILLRLGNIQW
                     QIGYLTVMGKGSKERIVPVGRSAYEAARVYVDEARPLFLKGASSDILFLTRWGKAFTR
                     QGLWKLIATYSRRAGLAKRVHPHTFRHSFASHLLEGGADLRSVQIMLGHADISTTQIY
                     THMTRERLKDIHRRFHPRG"
     gene            70038..72068
                     /locus_tag="PLW16_03080"
     CDS             70038..72068
                     /locus_tag="PLW16_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB69922.1"
                     /translation="MRRPDIPRGERWVDGKTKARRRWSAAAAGLLVPVLVLGGFWFGF
                     IIRDPQHDPIFGLKRVRNALSFYALGGPPRVYGLTGELNGRDFRLNPSETFTVTYRDE
                     FILHGVVSDDLLSRGVSVDVGGVGGERDQGRILRGVDLVDRAMAERGRTSGATDLPAE
                     PIRIYYRGTPVATIPVQAVVTPQDWLRHARASESLKERIDYLETALRMNPSDGNVRKM
                     LGGIYLRSGEVERAIGHYAEVVRQNPKDLPALRELLKAYAAGKNDAAVIRTGERLLAL
                     SPGEAAVYAHMAFACGNLRQWSRAVDLYRKSLALDPHPEMLYFRLGEAYEMTKDYPAA
                     IEAYRAVLKRTPEADYAWMAFAGANLKAGKNEEAIRVYREIIRRQPRNVLAHANLALA
                     LGNKGLPREEVAHNRKALAIDPGNTIVRFNLAAAYEKLTMDREAAREYRQVLKLKPED
                     ADALKGLADLEFRSGRYAAAAALYERLARVSPQKADVYAQLGFAYAEAKNYGLAAENY
                     EKALRFGKRDTQLRRQLADAYEKLGRKKEAIAQYERAAVARPTGEVLDVLADRYMAEG
                     RYEEAVQTYRKLIALNPRKSSNHEALGYAYERQGKNAAAAEAYQTSLKYDPENAEVFY
                     RLGLLHERNRAYEEALQAFEKAYELSPEHVRAGNKIPRMKILLLERKMGEKQ"
     gene            72065..>72461
                     /locus_tag="PLW16_03085"
     CDS             72065..>72461
                     /locus_tag="PLW16_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPB69923.1"
                     /translation="MKTKQVMVGSVALGGEAPLALIAGPCVLETEAKALEIARYLKTL
                     AGALEIGLIFKASYDKANRTSLGGFRGPGLKEGLEILAAVKRECDVPVLTDVHRFEEI
                     GPAAAVADVLQVPAFLCRQTDFVMEVARRA"
BASE COUNT        13852 a        21629 c        23089 g        13891 t
ORIGIN      
        1 agacgttcgg acagtgccgc gcctccaatt acctgcccct gctgcgcaag gccctggatg
       61 cggccggata cgaagacgtg ccgatcctgt ctttttccac cctcgacggc cagacgccct
      121 ccgggttcgg catcgaccgg aaaaagctcc tgcagaaact gtctctgacg ctggtttttg
      181 cggacgccct ggctcgcctg tacctggcga ccgttgcccg ggagcgctca cccggagagg
      241 cgacgcgcgt gcacaccgca tacctcgaac ggatggagga gggtctggcg cagggccgct
      301 tttcggtgct gctgcgcctg ctgcgcgagg cggtcgcagt cttcaacgcg gttcccgtcc
      361 atgaccgtgc actgcccgtt gtcggggtcc tgggcgagat attcgtcaaa cacaacgcct
      421 tttccaacca ccacctggtc tcctggcttg tggaacgcgg tgtggaggtg gtgatgccgt
      481 ctctggcggg atttttcctg cagcatttca tcaacgagga cttcaaccgc cgtgccgtcc
      541 tcaggcggat gggcggcgcg gaacgcctca tgctcgccct cctgaatccc ctgatccgct
      601 tccgtttgcg gcaggtggag gcggtcctgg aggggttccg ctacaatcgc cccgcccacg
      661 atctccgcga cctcgcccgg gaaacccgcc gggccatcag tctggtcaac caggcggggg
      721 agggctggct cctgacggcg gagatgatcg ccatgatctc ccacggcgtt cgacacattg
      781 tctgcctgca gcccttcggg tgcctcgcca accacattac gggcaaggga atcgaaaagc
      841 gtctgagagc cctgtatccg gacgtgaatc tcctcttcct cgacatggac gccggggcca
      901 gcgagatcaa cgccgtgaat cggctccatc ttctgcttat ggcggcgcac gaggccatgg
      961 ggacgagcga cgggtccatc ccggtcaggc gggtggtggt caatgcgtga ccctggcggc
     1021 gttttggccg aggcggaggg gcggacgact tgatcgggtg caatccggga aaagacgcaa
     1081 caagtcccgg agatgcttta gaagcggagc gaggttttcc cgcttcaccc catcgaaatc
     1141 ttcctgcatg aattttctca ggacttcatc cttctggatc gcccggccca cggacaccaa
     1201 ggcccggtcc cgggcgctca gggtccggtg aaccatgcgg ccatggaggg ggaaatgccg
     1261 gatgtccttc cggatgtccg cgggcaggct gaccttgtag agggcccgga gcacggggtg
     1321 atccgggggg gtgccggaca cggtaaacag aacgacggac tttcccccga tggcggacca
     1381 ggacgaagca atgaaaggcg ccacgacaat ccggccggtg cggacggggc tgccgaacac
     1441 gattacgtcg tacggcccga aatccgtcgt cggacacgct tcgagatcca ccatcacgct
     1501 ggggatctcc cggtggatcc agtccgcgta ctgtttcgtg ctgccgtagg tgctctggta
     1561 gaggatcagg acgcgtgccg gggatacgcc agatgttccg atcgcgcccg gggccgtcga
     1621 gacggtgcag acgggcgccg gggcgcaggc gcacagggga atccccgcca gaagcaggat
     1681 aagccaccgg gcccagcgac gctcacgggc gggtggaggt ctgaacgaaa ctgccgggat
     1741 atgcctcacc aatccgatga tccttgagat accggagggc atcgtcgcgg ttttcgaaac
     1801 ggccgacgag aacccgatac cacggtccct ttccctcgat gcggatcact tcgacccgcg
     1861 ccgtcgggta ctttgcccgc agcgcattcg cctgcgcctc ggccttcgcc gtttccggat
     1921 aggcgccgac ctggatgccg tagcgccctt tgggtacggc tttggcgcgg gttcgatctg
     1981 ccgcgggcgc cgtctcgatc gcggggccgg gtgtgggttt ctcccggggc atcgtgacgg
     2041 tttttaccgg tggggtcgcg acggcagggg gttccgccgc cggcgcggcg acgaagggtg
     2101 cgacgggtgg gggagggggt gccgcctgct caagcggttc ggccgggaga gccgttacgg
     2161 ggagcgcggc cgccgggagg ggcggatcga gccactgccg gccgtcggag tactgggcgc
     2221 gggacgtcag gcggttgatg cggagcgggt agtcctttcc gtccagtcgg acggtctcat
     2281 aatggtaaag gctgggccaa aggagaacga ggatcaagag gagtccagcc ccggcgaccg
     2341 cggccatcag ccctggacgg cgccggaccg cggtccccaa acgcgcttgg acggttttcc
     2401 ggggcgggat atcgtttccc ccgggagggt cctcgtcgtc ccggcccgga ttcacgggga
     2461 cggacaggat ggtcggactc ggttcgggcc gggacggatc ttccggagtg gccgtctcgc
     2521 gcgacgcatg caaaacgcgg agtcgatcat aggcctcccg gatttcactc tgcttctttt
     2581 ccgacgtctc tttccaggat tggacgtggg ggaaacggtc cgggtcccag gcggcgatca
     2641 ttttttcata ggcaaaatgc agctcggccg gagagcagtc ggggtcgatc tgcaggaggt
     2701 cgtagtcgag agccggatcc cacgaccgtg gtgtcggtgt cgggtcttcg gtcatagagg
     2761 cactcctttg cgggcatcca tgcgtgaccg gaaagtccgg gtggcttctc tcatcgggcg
     2821 gtcctcgggg gcaattcccc cgatcggtcg ccgcaagccg cggtcggaaa gcgatgtctt
     2881 tcccgcaggc ggcgcccatt atcgtggaaa gggtttttcg tgtcaatgaa atatacgggg
     2941 acgggtaatc ggaacgtccg acgccggcag ggtgccgccg tgcggcggga caatgacgcc
     3001 cgcaacggcc aatccggatt ctgaatcggt cgagaccagc tcggtggacg tggagtagcg
     3061 gatctactcg gggatccggt cacgcgcggc caacggggtc ctctcggcgc tcttgcacag
     3121 tgctacgaag agcgatttga acgcccctat ggtttcttcc gaccctatgt ccgcgaagtc
     3181 atctgccgat tcctggattg cggcgtgctg cgaaacggct ttgcccgggt gaaatgcaag
     3241 gactgcaatc acgaatacct gttggcattc tgctgcaaac gtctttaccg aggatcagga
     3301 gatcatcaag gccatcctgc aacatctcgg tctctgggaa tctcgctcac ggcttcccgc
     3361 gaaggcccat gcgcctcctc ccgcgctgtc agccattgag ggcgcgcgtg atcacctctc
     3421 tcgcgacagc cccgtctatg gggatcccat ttatccccgg gaggcttacg tataattcct
     3481 cgcccggtcg agaataccgg tggggtccgt cctttttcac ctaaaaaccc ctcatcgacg
     3541 ccttcgatca gggaacacaa catccctcgt gccattcttc cctctccctt cttccagaag
     3601 ccaatacccg aacccaaagc accacaacag gttgcgatag atttttcttg acgcacaaga
     3661 aggctgtcgt tattctttag ccactcgaaa gcaagttctt atcaaaaaaa attatctgaa
     3721 cgcaaaggca aataaagatg aaaaccgtct ctatgcgtaa atatattctt tgcggtttgc
     3781 tgctgatgtg cgccctgatt ccggtagacg ttttttgtgg agaaccggcc actgaggcaa
     3841 cgccggaaat tgtcaccaaa gtatccgccg ccgcttccga ctgggaaaat tccggattag
     3901 tggttaggaa gggatctaaa tataaaattt ccgcttccgg acggtggtcc ctggggccga
     3961 tctgcggatt taccgggccg gacggcgtgg gcaattcggc tatctgcggc ggagaataca
     4021 tagcgggaag cggttcgctt ctggtcggaa tgatcggaga gcagggagat ccttttgcca
     4081 ttggccgggg tattgaatta accgccccgg aagaaggtcc tctcttcttc aaggtcaacg
     4141 gtggccttaa aatattttgc agcggctacg ttacggttag aacctcaccg attgcccaga
     4201 cgccgtctgc cccgcccccg ctggcggaaa aagccgtgtc tgcggccaaa tcggatattg
     4261 acgaactccc ggcaagtaaa gccaaactaa ataaaaacgc ctatgctatc gtaatcggca
     4321 tagaaaatta ccgccagaaa ctgcctcggg ccgattttgc tgtaagcgat gccaggcttg
     4381 tgaacgaata tttatccaaa gtgatcggct acccggaaga aaatatcgtc atgctcacca
     4441 atgaacatgc cgccctgggc gacttcgtta aatattttga aaagtggctg ccgaacaatg
     4501 tcgaaaaagg cgggtcggtc tttatctatt attcgggcca cggcgccccg aataccaaaa
     4561 ctggcgatgc ctacctggtt ccctacgacg gcgaccccct gtttatcgcc gaaacgggat
     4621 attctttgaa aagaatgtac gaggctctgg gcaaacttcc ggcccgggaa actgtagtcg
     4681 ctctggattc ctgtttttcg ggagcgggag ggaggtcggt cattgctaaa ggcgcgcgtc
     4741 ctcttgtgat gaatattcaa gccaatctat ctctgtcggc caatattacg gttctttccg
     4801 cctcatcagg cgaacaaatc agctcaacct atgatgaaaa ggggcacggg ttattcacct
     4861 atttcctgct caaaggcatc aaggatgaaa atgtgattca acaggacggc tcgatcaaaa
     4921 aagaggacct ctttgactac atcaagcccc aggtggaacg catcgcgcgc aagcagtaca
     4981 acaatgaaca gacgccgcag cttgctggcc gcaagtgatt ttcggagata aacgcgggag
     5041 aagtaataaa caggcggcgc tatatctgcg cgggcctgtc ttgggttgcg ccacagacta
     5101 tttccgatcc agctcttttc gcacggccat acccagcttt tcgatgacgt aaggctttct
     5161 cacgtaggtt ccggcgccca gttccggagc cttatgcacc tgctccgact ccgaaaaacc
     5221 gctggcgata atagccttct gccgcagatt aatctcccga gcctttatgt aagtatccag
     5281 accgcctatt cctgagtcca taattatatc cagaagcagc aaatccacgg gatggttttt
     5341 tcgtgtcaat gaaatatcgc ggaggggcga tcggaacttc tgacactggc agggtgccgc
     5401 cgcgcgggcg gaacaacgac ccgtcggaga agaattccct tgacattaag ggtcagcttc
     5461 ggcaatgtgg tgcgcctcaa aggccacaac tgcttattaa aataaaggaa tcacctgaaa
     5521 cccggactgg ctccacagga tcgaccgacc gcagacggcg gatggaatgg aagaggagga
     5581 gcatgacgag attcacgaga cgcggatggg cgatgctggc cgtgttggcg gcgctcgtcg
     5641 gttcgcccgt actcctgtgg gcggaaacct ataccctaca ggacctttgt cggatcgcgc
     5701 tgcaacaatc cgagaagatt cgtatctccg aggagaacgt cgccatcacc ggcctgacga
     5761 aagacaaggc cctgtcggtg ctcttcccga ggttgacggc ctacgggggt tacgttcgtt
     5821 tcagtgagga taaatatacc gagccttcgc ggctgtttcc ttcggggcag atgatccagc
     5881 cgaacgcgac gtccgagtgg ggcatccgcc tggaccagtc cttttccttg agcgcccggg
     5941 aattgaccgc gctcaggatt gccaaaacta gcttcgagat gacccgatat acggacgagg
     6001 ccttcaagaa cctcctcctc gaggaagtgg cccgtgcgta ttaccaggtt ctgcaggccc
     6061 ggaagatcgt cgagatcgcc gaggccaacg tcgaccggtt gacgctttac cgacaggcgg
     6121 cggaaaagag gctcaaggtg ggcgaagtga cccggaccgt tttgcttcgg gcggaagggg
     6181 agctctccgg tgcccgctca gacctcctac aggcgcagaa cgcctgggag atggccaggg
     6241 cctatctggc ccggctcgtg ggtctacccg agatcgaggt gctccgggag gagacgggac
     6301 tggaagcgac gccgctggat ccgctcgaaa attttcagga caaggcattc cggatccgcg
     6361 ccgatctgaa ggcgatggat cggcagcggc agatcgccgc ggatcaggta cgctatgccc
     6421 gcggcgcgtt ctggccgaac gtggccttga cggccgctta tgcccgcgcg gatcaggagc
     6481 cggccacggc caccctcaac cgcgagagcc tctatggcgg cgttagcctg aattttccct
     6541 tcttcgaagg cgggctgaga acggccgagc tgcaggaggc ccgtaggcgc gagacgcagg
     6601 cggagctgtc ttaccaggat ctgaagaaga ccgtcgccat cgaagtgcag agcgtttatc
     6661 tcgacttggc cacccagcgc ggcgttgtga cgtttctcgc ggaccagctg gtctttgcac
     6721 gcgacaatta tcaggccgtc ggccgccaat tcagcttcgg tctggcgaac agcgtcgacg
     6781 tgatggacgc caacacgctt ctcgtttctt ccgagcggaa actggccgag gccacgtaca
     6841 gctaccactt gtctctgctc aagatggacc gggcgactgg aatcctctac cagaaagtga
     6901 tgggcgaccg gcgcgacgcg tcgccgaaag ccggcccgta gggatctgac gttcccgttg
     6961 aagaaaggga ggaccgtacc gacgatgcaa gcgtatccga aaagaaaacg aaccggtttc
     7021 actctggggc tgggaatggc ggcgatgctt cttcttccgc tggccggtgt gttcggctgc
     7081 cagaacaagg acgaggacgc gaagcgggaa cgcgtgctca acgttcgggt ccgggcggcg
     7141 gaggtgcaaa cggtgcgacc ctatatcgag gctgtgggtg cgctgaaacc ctacgaggaa
     7201 gtgatcgtca gcagcgaggt ggacggtatc ctccggcgcg tcctcgtgga ggaagggtcc
     7261 cagatttcac gggggacgcc gatggcggtg atcagcgaaa cggattaccg cctcgaggtc
     7321 agacgctccg aagcggccgt gcgtcaggcg gaggcgaacc tcgccaatat acgccaggaa
     7381 ttcgagcgca aagaggcgct cttccaggag gcgctcatca cgcggcagca gtatgacgac
     7441 gtgaccgccc ggcgcctggt cgccgaaggg gaggtggagc ggtccaggct ggccctcgcc
     7501 ctggcccgag agaggctggg caaggcgcgc gtccatgcgc cgctgtccgg ttccgtcaag
     7561 gagaaacgcg tcacggccgg ggattacatc cgcaacggga cgccgctttt tcttatcatc
     7621 cagacgaatc cgctcaaact gttgttcagc gtgtcggaaa aggacgtcgg cagcctccgg
     7681 gtcggacagg aggtgcggtt tacggtggac gcgtttccgg gacgcacgtt tccggggacg
     7741 gtccggacgg tgtatccgca tattgatgaa cggacgcgta ccctccaggt ggaggcggtc
     7801 attcccaatc ccgcccaggc gctcaagccc ggcctgtttg caagggtgac cctctactcc
     7861 ggcccggcgc gggaacaggt cgtcaccccg gtcacgtcgc ttctgtacga ggggcagaag
     7921 accaaggtgt tcctcgtcca gggagaccgc tcccgggagc ggttcgtcaa ggtgggccgg
     7981 aagtatgggg aggtgatgga ggtgctggaa ggcctcgagg cgggcgagcc ggtggtcgtg
     8041 gtcggccaga acaacctgac ggaggggatg aaggttcatg tggctcgctg acacctcgat
     8101 taaacggccg gtctttgcga cgatgttcat catggccctc gtcgtgctgg ggctggtgag
     8161 ctacccgtcc atcggggtcg atctcttccc caaggtcgat tttcccatcg ccacggtcac
     8221 gacgaccctc aagggggcca gccccgagat catggacgtg gacgtcaccg accggatcga
     8281 ggaggccgtc aatacggtca acggcgtcaa gaccatcacc tccgcgagcc aggagagcgc
     8341 gtcgcgggtg accatcgaat tcgtgctcga gcgggacatc gatctggccg tccaggacgt
     8401 gcgggagaag gtcgccgcca tccggtccaa gctcccggac gacatcggcg agccggtcat
     8461 cgagaaggtc gatgccgatt ccagtccggt cctgtggctc aacctctccg ggcagcggtc
     8521 gatccgcgat ctgtccacgt atgccgatga ggttctcaag gagcagctcc agaagatcaa
     8581 cggtgtgggc gcgctccgca tgggcggact gcggctgcgc cagatccgca tctggctcga
     8641 cgccgatcgc atgcaggcgt acggcatcgc cccgtccgat atcctgactg tcctgaagcg
     8701 ggagaacgtg gagttgcccg gggggcggat cgagagcctc tcgaaggagt actccgtcaa
     8761 gatccgggga gacattcggg atgcgtcgga attcaacgat ctggtggtgg cctacgtcga
     8821 cggcgccccg gtccggatcc gtgacatcgg acgggcggag gacgccctgg acgaacggcg
     8881 ctcgatcgcc aagttcaacg gcgttccctg cgtcggcatc ggcatccaga agcagtccgg
     8941 cacgaatacc gtcgaggtgg tcgaacgcat ccacgccgaa ctggagaaga tccggaagac
     9001 cctgccgacg gggatggagc tcaacgtcgc cgccgaccag tccgtcttta tcaagcgctc
     9061 catcgccgag gtgcagaagc acatcatcct ggggtcgctc ttcgccgtct ttgccgtctt
     9121 tctttttctg ggcaatgtcc ggacgaccct gatcagcgcc ttggccctgc cggtctcgat
     9181 tatctcgacc ctggccctga tccgcgcatt cgacttcacc ttcaacaaca tgaccctcat
     9241 ggcgctgtcc ctgtccgtgg ggctgttgat cgacgatgcc atcatcgtca tcgaaaacat
     9301 ccaccgccat atcgaggagg ggatgaagcc ccgagaggcg gcgtcgttcg ccaccgccga
     9361 gatcggtctg gccgtcatgg cgacgacctt cgccatcgtc gcgatctttc tgcccgtggc
     9421 cttcatgaag ggcatcatcg gccgcttttt cctgcagttc gccctgacgg tggtttttgc
     9481 cgttctcgtt tccctcattg tttcgttcac gctgaccccg atgatggcct ccctttacct
     9541 gaagccgaag acggcggtcg cgccggtgga tgccagcgac cggacgcggg ggatccgggg
     9601 ggtctggcgc cggatgggcg acgcgtggga acggggctac cggtgggtgg aggagctcta
     9661 ccggaggctg ttgggatggg cgctccgctg gcgcaagacg gtcctggcgc ttgcactggt
     9721 cctgttcgtt cttagcctct acatcacgaa gttcatgggc aaggaacttc atcccccgga
     9781 agaccaggga cagttcatcg tacggctcga agccccgatc gactactccc tcggaaaggc
     9841 cgacgagatg ttccgggagg cggagcggat cgttcgcggg ttccccgagg tccgggccgt
     9901 cttctatgcg caggggttcg gggccggcgg cgtcggtccg gtgaacaagg ccatcatgtt
     9961 caccagcctc atccccaagg cggagcgcga ccgcagtcag gaggacatca aggccgtcat
    10021 gcgcacccgg ctgaacgaga ttcccggcct gaagggaacg gcggaggacg tgtcggtcat
    10081 cggcggcggg atccgcaacg tgcccataca gtatgtcatc tccggcggcg atctgggcgc
    10141 gcttcagggg tacgccaagc agatcacccg ggagttcgcc agacttccgg gcatcgtgga
    10201 cgtggacacg tctctggagg aaggaaagcc ggagctcaag gtcttcatcg atcgcgacaa
    10261 ggcggcggac ctcggagtca gcgtggcggg catcgccgag gcggtgaaca tcctgatcag
    10321 cggcgaggtc gaaatcaccc gcttcaagga cgagaagaag ggccggcgct acgacgtccg
    10381 cgtgcagctc ggatccgagg accgacgcga tccgagcgac ctcggacgga tctatgtccg
    10441 gtcccgcgac ggccgccttg tccaactggc caacgtcgtg catgtccagg agggcggcgg
    10501 ccccagcatc atctatcggg tggatcggca gcgagccatc atggtttatg ccagcctgga
    10561 ggggaagccg ctgggccagg ccatggagga gctcgacgcc atctccgcga aggtgctgcc
    10621 ggccgacttc acggccaaat acaagggcgc cgccgacacg atgaaggagt ccttcggtta
    10681 tctgatgttc gccctctttc tcgggatcgt catggcctac atgatcctcg cggcgcagtt
    10741 cgaaagcctg ctccatccgg tgaccgtgct tctgtcgatg ccgctgtctt tcatcggggc
    10801 gttcggcgcc ctgatcctga cgggacggtc cctgaacatc ttcaccttca tcggcctgat
    10861 cctcctgatg gggttggtca agaagaatgc cattctgctc gtcgactaca cgaacaccct
    10921 gagggaacgt gggctcagcc ggcgggaggc catcctgacg gcggggccgg tgcggctccg
    10981 tcccatcctc atgacgacct tcgccatgat ctttgggatg atgccggtcg catttgccct
    11041 gggggagggg gcggaaacgc gctcccccat gggtattgcg gtgatcgggg ggctggtgac
    11101 ctccctgttt ctgaccctcg tggtcgtgcc agccgcctac gatcagttcg acgactggcg
    11161 gctggggttg agacggcgcc tccggaggcg gccgtccggc gcggacgcgg agagaaggga
    11221 agaggaaacg cgatgaagat ggtgctcgtg gtctacggat cggaagcgga cgaggaggtc
    11281 gtcggcgcct tcaagggggc cgggatcgtc cgctatacga aattctgcga ggcccgcggc
    11341 gtcgggacgg aaacggagcc gaaactggcc tcccgtgtct ggccgggcaa gaacaatgtc
    11401 ctgttcatcg gtgtcgcgga cgaggatatg cccggtgtcc tcaaggtcgt gcatgaactg
    11461 aagcggaagc atccccgggc cggggtcaag gcgttcgtca tgccggtcga agaacttgcc
    11521 tgatggacgg gacgttccgg gatacatgtc gcagcgacca tcgggatgcc gccgttcccg
    11581 agggcggcgt cacgacacgg ccaggacctt cgccccccag aatccgtccc gctttcaggt
    11641 ccgccacggc acggtccgcc tccgccaggg ggtagacctc gatccgtggc tggaggggga
    11701 tcctcgcggc gagttcctgg aattcgtcga tatcggcccg agtcacgttg gccaccgacc
    11761 ggatttcctt ctccatccac aggtaggtgg aataatcgag acggttcagt tagtcccggt
    11821 cgacctcttc cttgcggatg gcgttgatga tgagacgacc gccggaaagg ctcttcagag
    11881 cctccagaac cggccgccag gccggcgtcg tgtcgattac ggcggtcagg gggttagggc
    11941 tccgtgtcgc cggccccggc agcgcccagg tacagggcag agtgccgttc ctcctcccgc
    12001 cgcgcgaaga cgcggatccg tgtccgcggg tagcggtggc ggaccatttt cagtaccagg
    12061 taccccgaag ccccgaagcc gttcagtccc agaggttcca cgtcagtcag ttccgcgagc
    12121 gtgagcgacc ggtacccgat ggccccggcg caaagcagcg gggtcgcgtc ggtgtctgag
    12181 aaggcgccgg gaagcggaaa gtcaaaacct gcctggtttc ccgatggata gcggttcggc
    12241 ggccggaatg caagacacac ccgcgagatt cccgttgaag cgaaaggtca ttcctgatag
    12301 acattaaccg gatgcaggag ggtgggcatg gcgggggaga gactcagccg gagacagaag
    12361 gatatatccc ggctactgcg ggaaaagcgg cggccgatcc cggaccgccg ccgccaggag
    12421 aggaagcgga ggggagagcc gaggcatgga tgaaggcggg ttttccgaag acgatctccg
    12481 tcagatccgt gatgagggcc gggccgaggc cgaggttcgc cggcaatggg agaccttccg
    12541 gcggggggtg aggccgttga ccctagatcg tccctgccgg gtgggggacg ggattgaaac
    12601 cgttgccgcc ggggagatcc cgtccctgac ggctctctac gacgcggcca tggacgcggg
    12661 ccggatcgcg gtctttgtcc ccgcatcggg ggccgccacc cggatgttcc gggactggtt
    12721 tcgcgtcctg gaggacggtg acttcgcgtc ccatgccgcg ggggacacct tcgcgcgaga
    12781 gcttccggaa tacgccttct atccggatct actggccgtc ctcgagcgtc ggggaaccga
    12841 cgtggagacg ctgctgcggg agcgggatta cgccgcgatc ctcgaagcga tcctgacatc
    12901 ccgggggttg aattacggac gcctgcccaa ggcgctgctc aaatttcaca tcgacgcgga
    12961 ggggggacgg acggctctgg aggagcatct ggcggaggcg gcgctctatg cgggagacaa
    13021 cgaaggcgtc tgccgcgttc acttcaccgt ttcggaggag catctgcctg cggttcggga
    13081 ccacctgcgg acgattcagc cgcggtgcga ggtgcggctg gggctccgtt tccaggtgac
    13141 gctttcgacc cagaacccgg caacccatac cctggccgtc gatctggagg atcggcccta
    13201 ccgcgcgccg ggggggcgac tcgtctttcg cccggccgga cacggggcgc tgctgggcaa
    13261 tctccagtcg accggcggag acctcgtctt catcaagaat atcgacaaca tcgttcccga
    13321 tcgcctgaag gtccgcatcg tcccgtacaa gaagttgctc ggcggcgttc tcctttccct
    13381 ccagcgggag atcttccggt ttcaacggcg gctcgaagcg ggagacgctg gcgccgggga
    13441 acgcgaagag atgatccgct tctgccggga ccgacttcat gtttcgttgc ccgcggattt
    13501 tcccttccgt accccgatgg accagacggt gtttttgcag tcaagattcg cgcgtccctt
    13561 gagggtctgc ggcgtggttc ccaatgaggg ggagccgggg ggaggaccgt tctgggttcg
    13621 ggcgagcgac ggggcgacgt ccctgcagat cgttgaggcg tttcaggtgg attcggcgtc
    13681 cgaggcccag cagacccgct ggacggaggc gacgcatttc aatcccgtgg acatcgcctg
    13741 cggtctccgg gatgaaaggg gacggatgta cgatctcgac cgcttcgtgg accggcaggc
    13801 cggatgcatc accctgaagt cggaggcggg gcacccgatc aaggcgctgg aactgccggg
    13861 gctctggaac ggcgccatgg cgtactggaa cacggttttc gtcgaggtgc ccctcgaaac
    13921 cttcaatccc gtgaaaaccg tcaccgatct ccttcgcccc cagcaccgtc agggataggg
    13981 aaaggcgacc ggtgggctca gacgatccag gcctccgcat cctggcggtc ggccgccgtc
    14041 acggagatcg tcccgtcttg attgccccgc cggcactgaa cccgaacggg gacgcccccg
    14101 ggcgcgtctc cgtagagcac gcatagggac gccgcccgcc ggcacgcctc ctccccgccg
    14161 ccgcggggga tcaggacgga ggggcccgga aaggcgtcca tcttcagaaa acagtctccc
    14221 tcttcctccg cccgcaggtg ttcgatcgcc aggttgtccc gctggtcgcg ccccacgacg
    14281 acgcgctcgc cgcccggaag gcggaaatgc cgcccatgct tcagcagttc gatttcccgc
    14341 gcggaggcgt ccggccggtg ggcgaacaac tcgcgcaggc gtttcgaaaa cattgggtcg
    14401 gtgagcaggc agccgcccgc cggtgccgga taccgcgtga ttccgtagtg ctcggctaaa
    14461 gccatctgac gcttgcggct gcggccctcg atgtcgagaa gccgcgtgcg gtccacgagt
    14521 ccccgctgct ccagttccgt ctccggcagc agccgggcgc tcaggggccg cagcaccaga
    14581 gggagatagc cggagtgctt cgcgaccacg tagagggact gtttgttctg tgacatgggg
    14641 cgctggccca ggacctcgcc cgtcgcgatg aaggcggcgt tcatcgcttt aagccggtcg
    14701 ccagcgatcc tcagcatcag ggcgtggcaa tccaggcagg gattcatgtt tctgccgaag
    14761 ccgtagcggg gggcgcgcag catggcgagg tgttcgtccg tgatgttcat gacgaccagc
    14821 ggcaggtcga tcgagcacgc cgccgcccgg gccttggccg cgtcaaagaa gggggtttcg
    14881 aaggcgaggc ccatcacgtc gatctgctgg tcctgaagga tcttgacggc gaggatgctg
    14941 tccagacctc cggaaaatag agaaatggcc cgcgttttca aaatcggatg atccggatcc
    15001 ctccctgtgc gctcagacag ccgtaaagac cggggtcgat gtccgcctcc gtcagataca
    15061 ggatcctctc gccatcctcg attttcaggc ccgccaggtg gagggtgatt tgacccgacc
    15121 gtgctcccgg cggcggggcg aggctgaaca ggccggtcgt gtaggcgatg ctcatgccgg
    15181 tcaggacctg cgccaccacc ccggttttcc ccgccccgtc cttcagttgg gtgaaggaga
    15241 tgtccttttt ccgcagggcc gcaatgaact gggccggtag ctccttggtg cggaacatca
    15301 cggtccgtcc gttcctatcc acgagaagat cggcgttgac cgagaggttg aagccatcct
    15361 ccggggcgct gaagacccgg acggccgtgt tccgttgcgg ccggaaatcc agggccgcga
    15421 ggagggcgtg ggccagttcc agggtatccg tggacttgag gtgcgggacc tctccggcga
    15481 ccgccggttt gtccggacgg ggtgccacgc ggccgtccgc gagcacctcg atgatggaga
    15541 tgcccgaccg ctcaagggcg tgggaaaggg ctgcgggaag gaggtcctcg ggatcgagat
    15601 cggtgcgcag gccccacatt cggggtccct tggccgcatg aagccggccg gcaatcagcc
    15661 agtccggtcg aagaccgatc cggagaccgt cgccgccgga tatcgttctg aggttctttt
    15721 ccatgacgaa ggcccggctg cggctcaaga cggtcttcag gacggcgggg aggggttcat
    15781 ccggttcggt ccggacccag gattggcgtc cggcgttctt tccggtcagg ctcttgaagg
    15841 agtccggcat gcgggccaag gtgtcgagga aaatgacgct tccgtccgga agctccacca
    15901 cgggaaagag gccgcagtcg atcgtcatct gtccgccgtc ggggagtgga acatagtacc
    15961 agccggtcgt caggaccgaa ccctgcagac gggcgatcac ggcggagagg gcggcgagcc
    16021 ggtcggtcgc tggcgacggc aggaccggcg ccgcggcggg gggtgccgga atcgaagccg
    16081 gcggcgccaa gcccgcgctg ggtgaactct ccccgggaat ctccgggttc gtcgcaacgt
    16141 ttatcggaag atcaagggtg attgcgggca agtagatgtc ctgtccggga aggatccggt
    16201 ccggattctg aatgccgggg ttcgcctttt tcaggacctc cagggcgcgc ggcagcagct
    16261 cttcggggac gtgcagttcc cggcggagga tctggtagag ggtatccccg ctccgggccc
    16321 ggatgagacg ccggtcggca cgcaattccc ccggccgtcc ggtcggttcc gcgggaaaca
    16381 gccagaccag ctggcccggg gtgatctggt ggatgttcgt gagggacggg ttgaggtggc
    16441 ggagccgctc gaagtacttc agcttgcggg agtattcggt ttccccgtat tggcgggcga
    16501 cgatattgga gagggtgtct cccggctgga cccggtacgg gaccgtcccc cgggattgtg
    16561 aagccgatct cgtgaaggtc agccgggccg tgtcctcccg cgccggggcg gaggagggcg
    16621 ctattatcat gaccgccaga acggagagca ggagccgtcg ggcccatttc atcatcaagg
    16681 gtcctcgcat ggaacggatt tggccatgga tgggcgtgac ggggaaccac cgcgccgcca
    16741 cgagtcctct ttactgaagt caccgggaaa agtcaaacgg tggcgtaaag aaaatgccat
    16801 gcccttggcg ggcatggcat gggggcggtg ggaggggtgt tcagcggccg ctggcgaaat
    16861 cgtcgcggag gagttcgcgg gcgatgatca ttcggcggac ctcggaggta ccggcgccga
    16921 tgtgccacag cttctcgtcc aggtagtggc gcgacacggc gtattcctga cagtagccgt
    16981 agccgccgtg gatctggacg gcgtcggaca cgacggccgt cccggcttcg ccgcagtgga
    17041 gcagcgaggc cgcggccatc cggtcgaggt ccgtcccctt gccgccggac ttgtcttcca
    17101 ggctgtcgca gaaggcggcg gcgctgtagg ccatccattt cgaggagagg tagcgggtgt
    17161 acatgtcggc gagcttgccc tggatgaact ggaagttggc gatcggcttg ccgaactgca
    17221 cccgctcctt ggcgtacttg agcgagagtt ccaggcaggc gcgcatcgac cccagggcgc
    17281 atccggacag cgtgatccgc tcggtgcaca ggccgctggt caggaccttg acgccctggt
    17341 tgatcccgcc gaagaggttc tcctcgggca gcaccatgtc gtcgaaggtt acgagcccgg
    17401 tgggcgaggt ccgcatgccc catttcttga tcttctcgca gacgaatccc ttcgtggtct
    17461 tggggtagca gaagaaggtc gagatgccgt ggggcccctt gccgagatcg gttttcgcgt
    17521 agaagaccat gacgtcggcc acgggggcgt tggtaatgaa ggttttgctc ccgttgatga
    17581 cgtaggtgtt ccccttcttt tcagcgcggg tggtcatgct catcgcgtcg gagcccgcat
    17641 tcggttccgt gatgccgagg cagccgatgg attcgccgct gcacgccttg gggaggatgg
    17701 ccgccttctg cgcatcggac gcgtggcggc ggaagttgtc gaaggccagc gtttggctgg
    17761 cgccgacggt catggacagg gcgttgctga cccgggcgat gttttcataa acgagcagcg
    17821 tctccaggta acccaggccc gccccgccgt attgctcctc gtagacgatg ccgttcaggc
    17881 ccagcttgcc cagctcgtgg aacaggtccg gcggcatttc gtccttttcg tacaggtgtt
    17941 ccatctgtgg ctccagccag gtggacgccc agcgccagac gttttcctca agcatccgct
    18001 gctcgggtga aaattcgaaa ctcagagaca taaaggtacc tccctctcga tttatccttg
    18061 cggggtccga gatcctgtcg ggatcgaagc cggcaggcgg aaacccgttc aggtgcgggg
    18121 cgaaacaggc gttcccggtg ccaagcgccg tgtccgcagc ttccgggagg ggcttttgcc
    18181 gcacccccgg cggttcatcg ggagaggcgc gatgccagcg tacgatgcgt ccatcccgtg
    18241 tgtcgcgctc gtagcagata ttgattgaca tgtcaatcaa tattcggcgc gttgggccgc
    18301 cgggaggtcc ggcacgggga gataccggaa aaccagcgga gccatcaatt tctgggttgc
    18361 aagatcgccg tcagatgtta taatgttctg attccaatga tcggatcgct gtggtcggac
    18421 cggagggcgg tcctaccgcg tctttcgggg tttagaaaag ggcggtacag ggggagggga
    18481 tgggtcacgc ggaacgtgat gtgatcttgg ccagagagtt tcagcagcgg cttgagaaaa
    18541 agatgcgcga ggaggaactc tctcacctcg aatactggaa gacccagatt gattcgctcc
    18601 tggtcgcgcg gccggagggc gttgcggcgt tgcagtccca aatccgcaag gttgcggata
    18661 agatggccaa tcggattcag atgctgaaga agggcgcctg atgcaggata tcgcaaactt
    18721 tctatatgaa gtcggaatgt tgcaaaagac gccccggaca gggtttcagt tcctcggttc
    18781 gggaaaggag tccgtcgccg aacatgtctt gcggacgatc ttcatcggat acgtgttggc
    18841 tcgcctggac ccgtccgtcg acgagctcaa actgctcaga atgtgccttt ttcacgacct
    18901 cccggaagcc cgcacgggcg acatgaatta cgtcaacaag aagtatgttg ccgtcgacga
    18961 ggccaaggca atccgcgagt tgacggagac gctttccttc ggcggggaca tcgcggcggc
    19021 cctggaagag ttcaacgccc ggaaaacgcc cgagtccctg ttggcccgcg acgccgatca
    19081 gctcgccctg attctgcagt tgaaggaata tggggacttg ggcaacaagt acagccagga
    19141 gtggatccgg ttcgccctga agcgcctcgg cactgacgtg ggtcgcaggg tggcggaggc
    19201 catcctccaa accgactcct ctcactggtg gttcaaggac aagagcgact ggtggatcaa
    19261 tggaaaccac gaagggtagg gtcgttatga aaccgtcacc ccgtttttat cttttgatcg
    19321 ttctgctgct tccggtcgcg ttgggcgcgt gtggtcccac gccctggcag cgggacatgg
    19381 ctgaatccca tctgaagctg ggcatcgcac agatccaggc ggaacagtac aacagcgcct
    19441 tgaaagagct gatgaaggcg aatgaactca ccccggacaa tgcccgcctc cactattttc
    19501 tcggcatcgc ctatcacagc aaaggtctgc gcaagcaggc cgtcgaggaa ttcgagaagg
    19561 ccgtcgccct caaggccgat tattccgagg cgtacaattt cctcgggacg atctgtctct
    19621 ccgccgggca gtgggatcgc gccatcacgc tgttcgaaaa ggccctgacc aacattcttt
    19681 acgacacccc gggcgttgcg ctttacaaca tgggccaggc ctatcaccag aagggcgact
    19741 atcgcacggc cctcttgaaa taccaggagg ccaaggttcg ggaacccgat acgattctga
    19801 tgcccctgat cgagaagaac atggcgcagt cggcctatga actgggggag tatgccgagg
    19861 cggtggcaca tctgaagcgc gccatcgaac taacgccgga gctcgccgag gcccactact
    19921 ggctgggtcg tgcctacgtc aagatgggtc ggcgcgatca ggcggtgccc gcctttcagg
    19981 aagccgtgaa atgttctccg gcgtctgaag ccggcatcaa gtcccgtgag cacctcgatg
    20041 cactgcaggc ggaccgacgt tgaggggagg agaccctctt tccatgtcaa ccaagaaaca
    20101 gagaagaatc gttcaggttc aacagcgcaa aaagaagcag cagatgaaga cgtccctgct
    20161 cgtggccatc atcacggtga ccgtgggctt tctgatcctg ttcttcgtca ccctttttca
    20221 tttcgtgtat cccccggcgg cggggcggga cgccgcagcc agaagccgcg acaagatggt
    20281 ttgcattctt tattttgcgg acgccaacga acggtttctc gttccggaaa aacgattcat
    20341 cccccgggaa gatacccggg ccgcattagc agaggccttg gtcaaatcgc tcatcgaggg
    20401 gtctaagacc ggcttgacgg ccaccctgcc cgaaaaggcg gggctgcgca aggtcgagat
    20461 cgaaccggat cagacgcttt ccgtcagatt cgcgaaatcg ctccccgagc ttcacccggg
    20521 cagcaccacg agcgagctgg cgacggtcta ttccctgacc aataccctga ttgccaacat
    20581 accagagatc aaacgcgtcc ggattctcgc cggtgatgaa gtggccgcct ccattcgggg
    20641 ccacatcagc ctgaaaaacc cgtttgcatt caatccggag atcattgccc ccgtctcccg
    20701 cgcggactga ggggcgggaa gacggccctg catggcagcg aaaacaaggc tctccagaat
    20761 tagaaacatc ggcatcgtgg cacacatcga tgcgggcaag acgaccgtca gcgaacggat
    20821 catcttttat accggccgtt cctacaagat aggcgaggtc cataacgggg aagccgtcat
    20881 ggactggatg ccccaggaac aggaacgcgg catcaccatc acctccgccg tgacgacctg
    20941 tgcctggcaa aatcacgaga tccacattat cgacacaccg gggcatgtgg attttacgat
    21001 cgaagtggaa cgatcgctcc gagttctcga cggcgcggcg gtcgtgttct gcgccgtggg
    21061 cggcgtggag ccgcagtcgg agacggtttg gcgacaggca gacaagtacg gtgttcccaa
    21121 ggtcgccttc atcaacaaga tggaccgcgt gggtgctgat ttcgacttgg tgatcgctca
    21181 gatgcactcc cgtttccagt ccattgccct gcccgtccag atcccctggg ggcgtgaaga
    21241 ccagttccgc ggggtgatcg atctcatccg gcaggaggcc atcgcctggg acgacagcac
    21301 cctcggtgag gcctatcgat gtgtgcccat tcccgaggag ctgcagtcgg aggctagaag
    21361 ccgtcgggac acgctcatcg aacggctcgc ggaattggat gacgagctgg ccgagatgta
    21421 tctggagggg acggctgtcg cgaatgagga tctccaccgt gttctgcgtc aacagacctt
    21481 gaatttgtcc gtggttcccg ttttatgcgg atccgcgctg cgcaacaagg gaattcaacc
    21541 actcctggat gccatcgttc ggtatctccc ctcaccggaa gatgtgccac ccattaccgg
    21601 tacaaacccc cgcaccggcg aaaaggaggc gcgtccgagc agcgaaaagg agccgctcgc
    21661 ggcgctggcc tttaagatta tccaggacga ggggcgcaag atgacctatc tgcggctcta
    21721 ttccggacat ctgcaggcgg gggaggaggt ctataacgca agccgtcaga agaaggaaaa
    21781 gatcgcgcgg ctgctgaaaa tgcatgcaaa taaacgggaa cggatcgagc gggccgccgc
    21841 gggagatatc gtcgccgtta tggggctcaa ggacgcgggt acgggggaca ccctctgtga
    21901 tgagggccac cctatcattt tggagcctat cgagttctac gagccggtca tcagccaggc
    21961 catcgaggcc cgcacgccgg cggatcagga gcggatgtcc gtcgggctcg tcaaattgga
    22021 ggaggaagac ccgacactga aggtcaagta cgaagacgag acggcccaga tggtgatttc
    22081 agggatgggg gaacttcacc tggagatcat tatcgaccga ctccggcgcg agtttcacgc
    22141 agccgtgaat gtgggcaagc ctcgagtcat gtaccgggaa accatcacgg agagcgtcga
    22201 gcaagaggcg gtcttcaacc gcgaactggg cgagaagaaa cactacgggc aggtccggct
    22261 ccgcatggtt cctgcggagc gtggaggtgg gattcgcatc gagcgagcgg aaaccgcggg
    22321 cgcggccccc gaagaatatc tccacgccgt ggaggacggt atccgggaag ccgccgcgag
    22381 cggaatcatc tcgggatatc ccgtggtcga tatcgatgtg accgttctgc aggttccgat
    22441 ccgggaaggg gactcgacga cgctcggata caagatcgcg gcctcctccg ccttccgcga
    22501 cgggtgcggc caggccaagg ccgtccttct ggagccaatc atgagcgtgg acgtgaccac
    22561 cccgagcgaa ttcatggggg aggtcatcgg cgatatcaac agccgtcacg gcgaagtcca
    22621 gggcatcacc ccgcgggggc cgatcagcga gattctggcg cgcgtacccc tgaagggcat
    22681 gttcggttat tcgaccgatc tgcgctcagc cacccaaggg agggccgtct tttccatgaa
    22741 gtttctccag ttcgacaagg cctgagctga ggctgatcca gaaggaggcc gccggttagc
    22801 ggaaatgtcg atgaccaagc actccgagcc cacgggcacc ccatccggct tatcttccca
    22861 acccttgctg gaccgggaca aactcctcca gagcatcttc cgcatcagca ccctcctgac
    22921 cgccccggtc gatctggaca cggtcctgaa gcgcatcctt gacgaagtcg tcgatgccgt
    22981 cggtttcgac cggggcatca ttcggcttct cgatacctcg aaacgatttc tggaaacgaa
    23041 ggtggcgaaa aactacacgc cggaggaaga ggcgcgcgcc ttcgccgttc ccctgaatct
    23101 tgccgaacat gactgcgttt ccaccaaggt ggccaagaca ggggcctatc tggccatcga
    23161 ggatgccgcc gtcgatttgc ggatgaccga gacggaccgg atgctgacgc gggtctttca
    23221 cggggggtcg attttatgcg ctcccctcaa gatcgagcgc aacgttatcg gcgtccttgc
    23281 cgcctggcgt ggagagaaga cccgcttctt tcaggaagag atcgaccttt tcctgagctt
    23341 tgccagccag gcgagcatcg tcattcacaa tgcccggctc ttcgccaacc atgccgagaa
    23401 aattcgggaa ctgacccttc ttcaggaagc cgtttcggag atgaacttca gctatgatct
    23461 cgacaaccat attctcaatg ttcttatctc gagcgcgctc aaaatcggca aagccgaccg
    23521 ggtcgtggtg tacattctgg atatcgagac gaatcgctgc atgattcacg atggcgagca
    23581 ccagtacatc gaagcaaagg aggagggcga tatccgcctc aacaacacga tcatccagga
    23641 ggccctggcg gccaaggaga cccggatccg ctccgtcatc agcgggggag aggccggccc
    23701 ggtcatgttc gacggctacc cccacgagat cgccattccg ctggacatcc aggacaagtt
    23761 caatggagcg ctctacctgg ccaaacgcac ggaaggtttc agctccgacc agatcaacgt
    23821 ctttgacatc ctggcacgca acgccgccgt agcctatgac aatgccatca tgcactcgaa
    23881 gctttccctc gaggcgaaaa ccttgaagac ggaggtcgag aagctgaaag agcgggagga
    23941 ccggctgctg gggttccaca acattctggg aacgtcccag aagatgatcg ggatctttca
    24001 catcatccgg gaggtggcgg cccacgacac gaacatcctg gtgcaggggg aaagcgggac
    24061 ggggaaggag ctcatggccc gtgccattca ccgccagagc aaccggcacg ccaagccctt
    24121 cgtagatgtg aattgtgccg cgatcccggg gacgcttctg gaaagcgaac tcttcggcta
    24181 cgaggcgggc gcctttacgg acgcgcggaa gcgcaagatc ggtctgctcg agtatgccag
    24241 tggcggcacg atcctgctcg acgagatcgg cgacatgagc atccatgtcc aggcgaagtt
    24301 tctgcggatg ctcgaagacg gtcatatcag gcgactgggg ggcaatgaga acatccccat
    24361 cgacgtccgg ttcatttttg ccaccaaccg ggacctgggg cgcatggtga cggacggcat
    24421 cttccgtgaa gatctctact atcgcatcag cgtcgtcccg gtcgttatcc cgcccctaag
    24481 ggaacgggag gatgacattc ttctgctggc gaattattat ctcgatgagt tcaaccggaa
    24541 gttcaagaaa aggattaagg gattcagcct ggatgcggaa cggatcctgc gggcgtacca
    24601 gtggccgggt aatgtccggg agttgcggaa catcatcgag cgggtcatga tcctccagca
    24661 ggagggaacc atgatcgccc ccgaacatct tcccggcgag atcaagatgg ccgtgacgaa
    24721 atcagaaccg cagctccgga ccgatatcct gattccccgc atgtcgccgg aggggatcca
    24781 ttacgatctc gttctggacc gggtgaccag cgaggtgaaa cgcaagcttt tccatgaagc
    24841 cctcaacctg acgaagggga acatgacagc cgccgcccgc ctgctgggaa tctcgcgcta
    24901 caagttcatc cgggagcaga agaaactcga acaggccttc gaggagccac tggcccccgg
    24961 ttcggaacag atcgtgcgga attagcacat agcgtgcaga taccgaacag acgctcccgc
    25021 accgtggatc ggcgatttcc ccccaatcat cgttcgttac ctgtgcgaaa tgcgcacaat
    25081 cgacgcaggc catcgcttaa tttccttgcg ctttgacgaa cggaggagga ttgtctcggt
    25141 caagtcgcaa cgaggaaaaa ataaataagc ctaaagaaac ggcaagatac attattgggc
    25201 tgcccggagg acgcatcagg ggatagaact ggcattgatc ttgtatctac aaaagcaaaa
    25261 agagacatcg ggtgggcgaa aaacctttga attgaaattt atcgggttaa attttggaaa
    25321 ggtgccaatc ttagaggtct caccgcaatc atagcagcag gattcatgaa gccggaggaa
    25381 aaacgaagcg gtaggggaag tcatcgatta gcgtttccat cgaaacccga cagggcgacc
    25441 gaatatgaat tcttcatttc gggatccgcg gatgggggcg gtgacacccc ggagaaaaag
    25501 aacgccgagg agaatagggg ggagacaggt gcaacagttc tgggctcccg ggaggatgta
    25561 ggaaaagatc tctaaaatgt tttttatcgg tgggtggttt ctgttccctc ctttccaccc
    25621 accgaccccc cttaacgggt ctgaaaggaa tcggctgcat gcgacctttc agttttaatg
    25681 aatggcggat ggtttttggt tccctccttt tccatccgcc atttttttgt cctttttaca
    25741 cgttgaatcg aaacagcgcg atgtccccgt ccatcatccg gtaagatttc ccttccgacc
    25801 ggagaagtcc cttctcccgg gccggtgcga ttccccccgc ggcgagaaaa tcatcatagt
    25861 gcaggatctc cgcccggatg aacccacgct ccatatccgt atggatcctg ccggctgcct
    25921 ggtgagccgg cgtaccgcgg ggaatcgtcc atgcccggag ctcgggaccg acggtggtgt
    25981 agaaggtgat gaggttgagg agatcgtacc cggctcggat cagtttcgcc aggcccggtt
    26041 cggccaaccc catgtcatcg agaaagcctc ggcgctcctc cgggggcagt tcggcgattt
    26101 cagcctccat atccccgcag atcgtcacga caagcgaccc ctcctgggcg gcggcctgtt
    26161 ccaacgcctc gacggggggg gagggcgcct tcagcatcga ctcactgacg ttggccacgt
    26221 agaggaccgg tttatcagtc aggaggtgca tctcccgacg caaggcctca tcctccccgt
    26281 gctcttcatc ggggatcaga ctgcgggccg gcaatccacg cccgaggacg tcccgaagcc
    26341 gggtcaggaa aagcgtcgct ttgcgggcgc ccttgtctcc aaccttcgcc agacgctcgg
    26401 tcttctccag gcgccgctcg acggtatcaa gatcggcaag gatcagctcc gtgttcacga
    26461 cctcacggtc gcggagggga tcaaccgcac cgacgacatg gaccacgtcc gggttctgaa
    26521 agcatcgaat gatgtggatc agggcgtcca cctcccggag gtgtcccagg aacttgttgc
    26581 ccaggccttc cccgcgactt gcgcccgtga cgagaccggc gatgtcgacg aattccatcg
    26641 tggtcggggt gaccttcggc ggcgaaagga ttcgggcaag cgcctccagt cgcggatcgg
    26701 gaacgacgac gatcccgata ttgggatcga tcgtgcagaa ggggtaattc gcaacctcgg
    26761 cgccggccgc ggtcagggcg ttgaagatcg tggattttcc gacgttggga aggccgacga
    26821 taccgcagtt aaaccccatg gagaatcccg tccggtggtg gattgggctg gcccgcgggt
    26881 ttcatcggga gcgaagccgg agggccttga catccgggtc gatctataaa cgtaaaggct
    26941 ggaggtgtca aggccaagaa caatgaggaa gcgttccggg gacggggatc tgacgcgctt
    27001 caggggagat tatcgccgtg agatcgcgcg cccggttctt gcgagacgtg tccgtggaac
    27061 ggcgaaaaga aagggcggcc gattgacggc cacccctttg ggttcaggtt gatgcccacc
    27121 gtctcataaa cgacggctgg aatcggttac agcttctcgg ccgaaatccg catgccgtag
    27181 aaggaccggt aaacgaagat catggcgatc aggaagaaca aagccgccag acccgtcttt
    27241 agccccacgt tcgtcatggt cacggcgatc tccgtcgcga gcaggccgaa cagggtcgtg
    27301 aatttgatga tcgggttcat ggaaaccgac gaggtatcct tgaaggggtc gccgacggtg
    27361 tcgccgacca cggaggcctc gtggagcggg gtgttcttct gcttcaggtc gacctcgacg
    27421 atcttcttgg cgttgtccca gcagccgccg gcgttggcca tgaagattgc ctggaacaga
    27481 ccgaagaagg cgataccgat caggtagccg atgaagaagt aggggttgac gaaggcgagt
    27541 cccagggcca ggaagaagat cacgatgaag atgttgatca tccccttctg ggcgtagatc
    27601 gtgcagatcc ggacgacctc cttgctgtcc ttgatcgacg cttccttctt gttcaggtcg
    27661 atgttgtcct tgatgtagac gacggcccgg taggagcccg tcacgacggc ctggatggag
    27721 gcgccggtga accagtagat gacacagccg cccatcagga ggccgaggat gacctcgggc
    27781 tggacgagac tgagtttggc gatgacgtca ccgaacatct tctccaggag gatgatgatc
    27841 ccgaagacca tcgtggtggc gccgacgacg gcggtgccga tcaggaccgg cttggccgtg
    27901 gccttgaagg tgttgcccgc gccgtccgcg gattcgagac agtgtttcgc catctcgaaa
    27961 tcggggctga tgccgtagtg ctgcttcacg acgtccttgg catccttgcg ggattcgatc
    28021 atggagagct catagatgga ctgggcgttg tccgagaccg ggccgaagct gtcgacggcg
    28081 atggtcacgg gacccatgcc caggaagccg aaggccacca gaccgaaggc gaagaccggc
    28141 gcggcgaatt tgaacgccgg cggcatgatt tccatgacgg agggcgtctg ggaaacgaag
    28201 taggcgatga acatgaggac caggatcgtc aggccctccc agaaggcgga gaagttgccg
    28261 gccaccagtc cggacaggat gttcagcgat gcaccgccct ggcgggaggc attgacgacc
    28321 tcgtcgcagt gacgggaact ggtgctcgtg aagaccttcg tgaactccgg gatgagggcg
    28381 ccggcgatgg tgccgcagct gatgatgatc gaaagtgcca gccagagcgg catgccgccg
    28441 atgttgggca gatcggccag gaggaaatgg ctcgcccaga aggtgaccga gatcgagacg
    28501 atcgacgtga tccagaccag gttggtcagg gggtgctcga aattgaactg cttcttgtcg
    28561 ccgaacaggg acttgctcag aatgtcgttc acgaagtagg aagccaggga cgtaaggatc
    28621 ataaggatac gcatggcgaa gatccagacg atcagcttgc cgcccatgac ggggttgtcg
    28681 gccagggcca gggccaggaa ggtgatgagc gcaacgcccg tgacaccgta ggtttcgaag
    28741 ccgtccgccg tggggccgac cgagtcgccc gcgttgtcgc cggtgcaatc cgcgatgaca
    28801 ccagggttct tgggatcgtc ttcgggaagg tggaagatga tcttcatgag gtcggaaccg
    28861 atatcggcga tcttggtgaa aatacccccg cagattctca gtgcgctggc gccgagggac
    28921 tcaccgatgg cgaagccgat gaagctggga ccggccagtt ccatggggaa gtaggccaga
    28981 atgaggatca tgaagaagag ctcgatgctg accagcagca gtcccacgct catgccggaa
    29041 cgcaggcaga tgttgacgat attcaggggt tggccgctca gggatgcgaa agcggcgcgt
    29101 gagttggcga ccgtgttgat ccggatgccg aaccaggcca ccccgtaaga accgagaatg
    29161 cccgcgatcg aacacaggag aatgacgatg atgctggtcg catccatttg ggacaggacg
    29221 ccgaagtaat agagcataca gagggcgatc aggatccaca gaccggcgag gaacttgccc
    29281 tgctgcagga cgtaggtctt gcaggtttcc cagattgtgg cggagacgtc gagcatcgct
    29341 tggtgggccg gtaggcgttt cgtctgccgg tactgcatcc agccgaacgc catcccgata
    29401 acgcagacaa tgagcccgag tttaagcact aaaagcccgt tcagtgtccc gaacatgacc
    29461 agggacaggt cgggcagtac gatgtccgct tcgctggcaa gggctgccgg cgtcatcatg
    29521 agcaacatga tgaatacgga gcataaaatg gaccaaaaac gtttaccctt atgcatcatt
    29581 aaacctcctc attagaattt tttatggctt tgccattata tttccccatt gccgcccgga
    29641 ttccggatga aacgatctcc ccgatcgctt ccaccgccgt ttccagcagc atggacaacc
    29701 gatccttttc ctcgggcgcg aagggttcca gaacgtaccg ttccacgtct tcccgggccg
    29761 tcggccggtc gatgcccatg cgcacccggc tgaaatcgcg tgaacccagg tgctgcataa
    29821 cggagatcaa ccccttgtgc ccgccgtggc cgccgccgta tttcagcctg atttcagcga
    29881 agggcagatc cagatcgtca tggacgacga cgacatcttc cgtcgcgatt ctgaagtaat
    29941 caaagagctt gcgcaccgcc tgaccgctca ggttcatgaa ggtctggggt ttggccagca
    30001 acacggcggc gccgcccatc gcaccttttc cgacgacgct gtcgaaacgg ttgtcggtca
    30061 gggggatgtc atgcgcctcg gacaaccgct ctattacccg gaagcccatg ttgtgccggg
    30121 tcttttcata gcggcgcccc gggttgccca gaccgatgat caggtgcacg gtctcctccc
    30181 tgacggggaa tggctactcc tccttggcct tcttgtcgtc gctgatccgc tcaggttcct
    30241 tcacggcctc ttcctctccc tctggcacga cgactttggc ttccttcttg gcgctcaccg
    30301 cgaccacggc gacgtccgcg tggtccagga cggtgagacc ttccggaatg gccaggtcgc
    30361 cgatcttgag cgcatcgccg atctcgacgt tgctgatgtc cacggaaatc gcctccggaa
    30421 gccgtgcggg caggcaggaa acctttattt cccgcttcag atgctgcagg tcgcctccgt
    30481 tatttacgcc gacggggata cccgaaaaat gcaacgggac atcgaaagtg aaggcctggt
    30541 ccatgctgat ctcgtagaag tccacgtgcc ggagcttgcc tgtggtgggg ttcgactgca
    30601 tctccttaat catgacgagc ttttcacggc tctgtccggc ctcgtcgaac gacaacctga
    30661 taaagatgtt ctcatcgccg ccgcgcagaa gtttcatcaa ttcggcggtc ttgaccgcca
    30721 gcatttggtt ctgggtcttt tgcccgtaga atatggccgg gatcgaccct acgtttcgca
    30781 gccgccgcgc gggtcctttg ccggcttcct gacggatctg gactttcagt tccgctgtct
    30841 ccataaagac tgcctcctaa atgaaaagtg aactcaccga atcgttatag taaatcctgc
    30901 ggacggcttc gctcaaaaga acggcgaccg agaggacctt gatgcgctgg catgccaggg
    30961 cgcgttcgtg aagcgggatc gtgtccgtga cgatgacttc cttcaggtcg gacgattcca
    31021 ggcggtcaat cgccggtccg gagagcaccg ggtgcgtgca gcagaccgac acctccaggg
    31081 agccctgttc cgtcagggct ttggcggcac ggacgacggt tcccgcggtg tcgatcatgt
    31141 cgtccagaat gatgaccttc ttgccttcca cctgtccaac gatgttcatg acctgcgcct
    31201 cgttggggcc ttcccgccgt ttgtcgataa tggccaggct cgcgcccaga cgggagccga
    31261 aggcgcgcgc ccgctccacg ccgcccgtgt cgggggacac gatcacgacg ttatcgctgt
    31321 agttgcgttt catataatcg aggaggaccg gcgtagcgaa gaggttgtcc acggggatgt
    31381 tgaagaatcc ctggatctgg ccggcatgga ggtccatgga gaggatccgg ttcgctccgg
    31441 ccgtcgtcag gaggtcggcc acgagctttg cggagatcgg cgcccgcggc gccaccttgc
    31501 ggtcctgtcg cgcatacccg taatagggga tgacggccgt aatccggtcg gcggaggccc
    31561 gtttcatggc gtcgatcatg atgagcaact ccatgagggt gacgttgacc ggggagcagg
    31621 tcgactggat gatgaagaca tccatgcccc ggacgttttc gtcgatctcg acgcgggttt
    31681 ccccgtcgct gaaggtggtc acatgggcct tgcccaggga aacgccgagc ttctgacaga
    31741 tcctttccgc caggggccgg ttcgcattgc cggagaacag tctgattcgt tcaagcattt
    31801 atcgcctcga tcgtgtgcgg atagtctggc tggggcggga ggattcgaac ctccgaatgc
    31861 aggctccaaa ggcctgtgtc ttaccgcttg acgacacccc aacgccgatg catcagtaaa
    31921 ggggatgtaa gaggccgtct tcagagcgtt cgggccacaa acacggaata gggtgagaac
    31981 cgcttcagcg cgacctccgc ggtttctgcg ccgtcttccg tagcgaaaag cccgaagacg
    32041 gtgggaccgc tgcccgacat gagcgcccct atggcgccga ggtcgaggag atatcccttg
    32101 atgtccgcca attccggatg aagccggatc gaaaccttct ccagatcgtt gtaaagaccc
    32161 gctgcccaat cacccgcttc acaaaatcgc gggatgctat aatttattga ccggtttgtc
    32221 aatctgaaat tgagtttttc ataaatgatt tttgtggaca aggcgaaccc gggattgacc
    32281 agaacgaacc agaagcgggg gacctccgga gcgactttca ggcgatcgcc gatcccgaag
    32341 gcccaggcgg tacgaccgaa gatgaaaaac ggaacgtcgg cgcccagtgt tgcgccgaca
    32401 tgaagtaact cctgaaggtt acaaggggat ccgatcagtt cgttgacggc cagaagggtt
    32461 gtcgccgcgt tcgagcttcc cccgccgaga ccggcggcga gggggatgcg cttcttcagg
    32521 gtgatgtcca ctcccccggg gtccggggca tgggtgagca gggccgcggc cgcgcgccag
    32581 acgatgttgt tcccgtcttc ggggagggtg ccgtcggggc aatgcagaga aacgccctcc
    32641 gaccggcggg cgaagctcat ctcgtcgtag aggctgatcc gctgcatgag cgtggccaag
    32701 tcatggtagc cgtccgggcg ctttcgcagc accttgaggt ggagattgac cttggcgggg
    32761 gacagcctgg tgatcatcgg gtgcgctttt ccttgacgta gcgcattctc agctcgaaga
    32821 gcaggccctc gagaagctgc ttttctgcgt ccttcaggtt cccctccgtc ttgtccctta
    32881 gcatgcccag gatgtcgatg gtttgcttgg ctgcggagag gttgcgccgg gtttctccgg
    32941 tggtggggtc gggaaaatca ccgaaatggt acatggccgt cgtgctcagg gagaggatga
    33001 aattctggaa atcaatggcc ggataggtct gctcttcccc gggcgcgtcc tgtggggacg
    33061 cgggggcggc atccgtccgt ccgggcgctt cggaggcggt gtcgggcgga accgcgccgg
    33121 tttccgccgc gggtgggggc atggttttgg cctcttcggt ccggacctct ccggactcgt
    33181 cgaaatggcg ccgatccttg atgacgaaac ccgtttcttt cttctcttct tccatgtcaa
    33241 cctcccaaaa cggctgatgg cgtcaggcgc ggggcgttag gatttccgtc ccttgcccgc
    33301 cgcccgtctc gttacatcgg tctcatgctg cgcagccgcg cgatccgctc ctcgatgggc
    33361 ggatgggtgc tgaagaggtt catcagcggc cgccccgtca ggggattgac gatgaacatg
    33421 tgggccgtgg ctggcttggc gttcatgggg agggcctggg aggcccggga gagcttttca
    33481 agagccccgg cgagtccgta gggcttcccc gagatacgcg ccccgccctc gtcagccatg
    33541 tattcccgtg agcgagagat cgccatctgg atgatcgtgg ctgcgatcgg ggcgaggatg
    33601 gccgtgagga tcaggccgat cattcccccc ccgctgtcat cgtcgtcccg gctcacgccg
    33661 ccgaagatgg ccgcccactg ggccatattg gcgatcatga cgatcgcccc ggccagcgtt
    33721 gccgcgatgg agccgacgag gatgtcccgg ttcttgatgt gggtcaactc gtgggcgagg
    33781 acgccctcca actcgtcccg gctcaggatg cgcaggatcc cctccgtgac ggcgacgacg
    33841 gcgtgctttt cgttacggcc ggtggcaaag gcgttgggcg tgtcctgcgg gatgatgcag
    33901 acccggggca tgggcagcga ggccttcatg gacaggtccc ggacgacgcg gtagagctcc
    33961 ggcgcctccg cttccgtcac cggctgggcg cggtacattt tcaggacgat cttgtctgaa
    34021 aaccaatagc ttccgaaatt catgatcatg gcgaaggcaa aggcgatcac gagccccgtc
    34081 cgaccgccga aatacccgcc gatcagcagg agaagccccg tgagggcccc gagcagcaag
    34141 gccgttttga atgaattcat ctgtccctcc gtaggcaaaa ggtcgaaggt caagggccgg
    34201 gttctttcaa cctgttcgcc ctttgccctt gatcctcttg atgcctgtta tttgaaaatg
    34261 atcttttccc cttccacatc gacggtaatc cggtcgccct cccgccagga ggattccagc
    34321 agatccatgg ccaagcggtc caggacgagc ttctgcagga cgcgcttgag gggtcgggcg
    34381 ccgtagacgg gactgtaacc tgcctcggct atatagtttt tcgccgtctc tgtcaattca
    34441 atggacaact tccgctcggc aaggcgcttt tcgatcagcc ccatctggat atcgatgatg
    34501 cggtcgatgt cggccagcgt cagggaccgg aagatgacga tgtcatcgat ccggttgaga
    34561 aattccggct tgaaggccgc gcgcagcgcc tccatcatgc gttcgcggcg cgcgggttcg
    34621 tccccggagg gatcttggag ccactggctg ccgatattgg acgtcatgat gatgatggtg
    34681 ttcttgaaat ccactgtccg tccgtgcccg tccgtcagcc gtccgtcgtc caggatctgg
    34741 agaaggatgt tgaagacgtc cgcatgggcc ttctcgatct cgtcgaagag gacaaccgtg
    34801 tagggcctcc tccgaacggc ctccgtcagg taaccgccct cgtcgtagcc cacatagccc
    34861 ggcggggcac cgatcagccg cgaaaccgag tgcttttcca tgaactcgga catgtcgatc
    34921 cggatcatgg cctgatcgct gtcgaacatg aactcggcca gcgccctcgc cagttccgtc
    34981 ttgccgacgc cggtcggccc catgaagatg aacgagccga tcgggcggtt ggggtcctgc
    35041 agccccgcgc gtgcccgtct cagagcgctg gagacggcag caatgccctc atcctgtccg
    35101 atgacccgct tccggaggcg tttctccatg tggatcaatt tttggacgtc ggattccatc
    35161 atgcgggtga cgggaatgcc tgtccagttc gcgacaacct ccgcgatgtc ctcttcatcg
    35221 acctcttcct tgagcatttt ctggtccttc tggacatcgg cgagggcgaa ctgctccgct
    35281 tcgagccggc ggtccagctc cacgcgcttg ccgtagcgga tctcggcggc acgcgccaga
    35341 ttgccctccc gctgggcgtt cagttcgtcg gtcttgagag cctccatctc ctccttcaag
    35401 gtttggattt tcttgatgga ttccttctcc gctgaccagt ggcgcttcat ccggtccatg
    35461 tcgtcccgga tatcggccag ttcccggtcg atcttttcac ggcgctccag ggttgtcgga
    35521 tccttctcgt ccttgaggga ctgccgttcg atctcgagct ggatcgttcg gcgctcaagg
    35581 acgtcgatct ccgcgggcag gctgtccaac tcaatgcgca ggcgcgaggc agcctcgtcg
    35641 acgaggtcca ccgccttgtc cgggaggaac cggtcgctga tgtaacggtg ggacagcgtg
    35701 gccgcagcaa tgatggcggt gtccttgatg cggaccccgt gatggacctc gtagcgctcc
    35761 ttcaggccac ggaggatggc gatggtgtcc tcgaccgtcg gctccttgac gaggatgggc
    35821 tggaaacggc gttccagcgc gggatccttt tcgatgtgct tccggtactc gttcagggtc
    35881 gtcgcgccga cgcaacgcaa ctccccgcgg gccagggccg gcttgagcat gtttgaggcg
    35941 tccatggagc cctccgccgc cccggcgccg acgacggtgt gaatctcgtc gatgaagagg
    36001 atgatctccc ccgcggcggc tgttacctcc ttgagaacgg ccttcaaccg atcctcgaat
    36061 tcgccccggt acttggcccc cgcaatcagg gcgccgatat cgaggccgac gacccgcttg
    36121 ttcttgaggc tgtccggaac gtcgccgttg acgatgcgct gcgcgagacc ttcgacgatg
    36181 gccgtcttgc ccacccccgg ctctccgatc aggacggggt tgttcttggt ccgccgggag
    36241 aggacctgca tgatccgccg gatctcctcg tcccgcccga tcacggggtc gaaagccccc
    36301 ttcctggcga tctcgttgaa gtcgcggccg taacgcttca gggcctcgta tttttcctcc
    36361 ggattggggt cggtaatccg ctgactgccg cggatatcga ccagcacctg aaagatgcgg
    36421 tccttggtca cgccggaccg cttcaggatc gcggggatgg ccccgtcctt ttcttccgtc
    36481 atggcgagca ggaggtgttc cgcgctgacg tattcgtcct tcaggcgcgc cgcctccgtg
    36541 agggctcggt ccatgatgcg gttcaaccgg ggactgaggt acacctgggc gccggcgtct
    36601 ccggtcaccc ggggcagttt ttccagggcc tggcggcatt ccgcctccac ggccgccgga
    36661 tccgccccca gtttcttcag gatggctccg acgatgccgt ccggctgcgc caggcaggcc
    36721 ctcatcaagt gctcggcgtc gatggcctga tgaccgtacg ttgaggccag accctgcgcc
    36781 tcctgcagcg cctcctgcac tttgagtgta aatttatcga atcgcattga tcctccatcg
    36841 gatgaaggca cgggaacaac ggccaaagga gacctaaacc ggccccgata tccgtctgtt
    36901 ccttgtcctt cgccttgtgc tttttttatg cttttgctcg atcggtttct cgatcgtgac
    36961 gaagatccgt ccgttcttga agatgctcac ggtccccaag gattccgacg tgacgatgga
    37021 aatggcatgc gtgatgcgtt gtcatgccgg ttgcccaaac ttcgcctcga tccccctgac
    37081 gatctgacag gcaaagtccc accagagtct tgttgatcga cgcccagcag cctcccggtg
    37141 agacgcgtgt gcggcctcaa gattcggatg taaggtatcc accgacccgg agaatgtcaa
    37201 ggaaacgaag cgtcggacgg cggtgggcgg ggcgtccacg gcaaatattg cttgacgtgg
    37261 gggaaggaag cagattatac accgcaagaa tctgatattg tttggtaaat ggagacgatc
    37321 ccgtggattt gcctgctgtt acgagcacgc tcgatctata catcgctgaa atcaaccagt
    37381 ttagccttct cacaccggag gaggaattcc gtctggccgt gcggttaaag aaatacagcg
    37441 atatggaggc ggcggaactg ctcgtcgttt ccaacctgcg ttttgtggtg aagatcgcgc
    37501 acgaataccg caactacggt ctcaagctgg ccgacctgat ccaggagggg aacatcggcc
    37561 tgatgcatgc cgtcaagaaa ttcgatccct accggggcta ccggctcatc tcctacgccg
    37621 tctggtggat ccgggcttac atgcaaaatt tcatcatcaa gaactggagt ctcgtcaaga
    37681 tcggcactac ccaggcgcag cgaaaactct ttttcaagat ggggcaggcc aaaaagcagc
    37741 tcgacgccct gtccgaaaag catccggagt tctctgaaat cgcccagtcg ctgggtgtga
    37801 aggagatcga actcgcagaa atggatcagc ggatgagcca tcgcgacgtg tctcttgacg
    37861 cctatgtcag cgacgagggg gacgcggcgc acatcgattt tctcaccgat gacggtgaga
    37921 atcaggaaat ggccctgatc cgcaaggagg agatgcaact ggtccagcgc aacatcgccg
    37981 gcgccatggc caatttgaat gaaaaggaga gttacatcgt caagtaccgg atcatggcgg
    38041 atcaccccca gacgcttcag gagatcggca accgctatag cattacccgg gaacgggcaa
    38101 ggcagatcga gaagcaggcc ctgaagaagc tgcggctagc cctgccttac ctgggtgagc
    38161 ggacggagct gattgagggg taggccacgg gggaaggcct aagggggata gatccgggtc
    38221 gaggggggca aggccgcacg aacggcagct cattccgtgc cgctcgcgag gttctcgaag
    38281 caggtgtact tttccaggaa ggccagctta accacgcccg tcgggccgtt ccgctgcttg
    38341 ccgatgatga tctcggcaat gccccgctcg ggattatcct cggacttgtt gtaaacttcg
    38401 tcgcggtaga tgaaggcgat gacatccgcg tcctgctcaa tggcgccgga ctcccggaga
    38461 tccgccatct ggggccggcg attggtccgg tcctccacct tccggttcaa ctgggacagg
    38521 gcgacgaccg ggacgttcaa ctccttggcc agggccttga gcgagcggct gatttccgag
    38581 atttcctgtt cgcgcgattc cctgtaggcc ccgccgcgca tcaactggag ataatcgagg
    38641 aggattagtt cgagcccgtt ttccgccttg aggcgccggg ccttggcctt catctccagg
    38701 acggtgatgg ccggcgtgtc gtcaatgaag atcggcgcct cggaaagcct gcccgccgcg
    38761 ccgacgagct tttcccaatc cgtttcccca agaaagccct tgcggatgcg ttgcgaatcc
    38821 accttggcct ccgaggcgag catgcgcagg gcgagctgtt ccttcgccat ttccagagag
    38881 aagatcgcca cggggattcc agattcaagt gcggcatggc tggcgatgtt cagggcgaag
    38941 gcggtttttc ccatgctggg acgccccgcg atgatgatca ggtctgattt ctggaagccc
    39001 gaggtcaaat cgtcgattcg ctcgtatccc gtgggcacac ccgtgaccat ttccttgttt
    39061 tcatagagat gctcgaccgt ctcgatgctc ttcttgatga tgtcccggaa ggggtagaag
    39121 gacggccgga tcttgttctc ggaaatctcg aagatggcgt gctccgcctc gtccagcacc
    39181 tggtccacgt cggcgccggt ctggaagctg ttggtgatga tttccgtggc ggttccgatc
    39241 aggccgcgga ggatggcctt gttcttgatg atcttggcgt aataggcgac attggccgcg
    39301 gacggcacgt tgtccaccag ggaggccaga tagaccgctc cgcccaccga gtcgagctgc
    39361 ttgcggtctt tgaggcagtt gctcagggtg atcaggtcga tcgggtcgct gcgctcggag
    39421 agggcgagga tggcggcgaa gattcgccga tgggcctcgc tgtagaaatc cttcgcatcg
    39481 aggatttcca aaacggcatt gagggcatgg ttctccagca ggatgccgcc gaggacggat
    39541 tgctcggcct ccaggttttg cgggggcagc ttgtgcaggg tcggatccat ttcgctcatg
    39601 acgctcaggc ttccgcgacg acgcatacct tgatttccgc cacgatgccc gccggcagct
    39661 tgatcttcac gtcatactcg ccgacggctt tgatgggctc ttccatctgg attgctttcc
    39721 ggtccaccgc gagtccccgc tcctggagcg cgccctcgat gtccttggtg ttgacggaac
    39781 cgaagagttt gtcctgctcg ccgacccggc gcgcgatcgt cagggtgagg ccctggagcg
    39841 aagccgcggt tgcttcggcg cccttccgga tcttttcggc ctgttggaag atgtgttttt
    39901 tttgctccgc gaacaccttc gcagtcttct cgttggcctc gacggccaga cctttgggga
    39961 tgaggaagtt ccgggcatag ccgtccgaca ccttgacggt atcgcccaga tttcccagcg
    40021 atgcgacgtt gtccttcaaa atgaccttca tgaagacctc ctggtgatgg attcgggtct
    40081 gttcaggtta aagggacgga ttgtccctcg gtttgatgaa acggcgaaag tcgacccaca
    40141 ggtcaaacat accaaaggcc gcgacgatca gcaacagata ttgctgcagg accaggagtg
    40201 cgtagaaaaa aaagcggagc caggcaggaa cgcgtttcat acggaagagg aagcccgtga
    40261 tcgccagccc ctggaggagg tagacaaagc cgcagagaat gaggacattg accccggcga
    40321 tctttgcccc cgcgtgcggc accagagcca gccccccccc gataatcagc agccagatga
    40381 gcccctcggg cgccttccag cccgagaggt ccggattgcc cagcgccgac gctttccccc
    40441 ggcggagcag gaagcgggcg gcaagaaggt tggcccagac ggaaagcacg atgccggccg
    40501 cggcgacgga agggaagatc gccattagaa agcgctggaa ttgtggcgcg ttgtcacgga
    40561 tgatggcgac ctgttccggc ggaagatcga gttccgaata gaccttcagg ttttcccgca
    40621 ggctgtccga caggtgcgtg gcgacgatgt gccagggggc ggaccccgtt cgcaggctct
    40681 cggccagaag caggaagagg ctcaaccctg cgaggacggc agacccgatg gccagggttc
    40741 ggacaaaatc gaccccccgg acagtcagtt ccccgaggac caggccggtc agggaagcgc
    40801 tgagcagaac gggtatcagg ttgagcggac gggaggaggc gaggagaatg aacgtcgtcg
    40861 cagccagcga gacaaaacag accaccaccc cccgccaacg tcccaatttt gcgtaaaaga
    40921 gaagcgtcgg caggggcaga aagacgatga cgccggtccc tcccgccggg atcagggcga
    40981 cgccgacgaa aaaaagggtg gaaacggcga ttccgatccc aagatccaga tgcgaccgta
    41041 aacccgtgcg atcgttcaac tccccgtccc gctgatgagg cccgctcagc cttcggtgac
    41101 ggcgaacggc atcagggcga tgctgcgcga cctcttgatg gcggtggtca gttcacgctg
    41161 gtgcttggcg caattcccgg tgatgcgtct gggcatgatc tttccccgtt ccgtcacgaa
    41221 gtcgcgcaga acgtccgggg atttgtaatc gatgatcaag gccgagtccg tgcagaaacg
    41281 gcagaatttg cggcggtgga aaaatcttct ctgaggcctg ggcggacgct ggtctcttct
    41341 ttcgggtacg gccatgttac ttcaccccct cattgacggt ttttgcggcg gtttcctgag
    41401 cggcagcggc gggaatcacc atctttggcg caatcggttc ggccggctgc ttccccgcgt
    41461 ctccctcttc ggctttcttt gcggcgtcgg cgatttcctt ttcaatcgct tcgcgggtga
    41521 aggtcccgtc cctccggacg gtcagatact tcagaatccg ctcatcaatc ttgaaatttc
    41581 tttcaatctc ggtgatggcg gcatggtcgc tgacgacgtc gaacagcagg taatagcccc
    41641 gtctctgttt cttgatttcg taagccagtt tgcgcttgcc ccagcggtcg atcttgacga
    41701 cgatgccctg gtgatcggtg atgaacgtgc cgtaacgctc gcacatcgcg ctgatttcgt
    41761 cctcgggcag atcgaccggg acaatgaaaa tgatgtcgta tctcctcaat gcgcttcctc
    41821 ctctcggctc ttaagtccgg gcacgggacc cggacaagga gtgttcccgc gggtctgcgg
    41881 gaaattaaca cgccgatcgc aggggaatga aggccgccct gaatcgcgtg tctccaaaaa
    41941 atgaaccggg cctataccat gagacgaaaa ggcatgcaac cattatttcc gcgctcccga
    42001 cgcggtcagt cccagggggt tagcgtttag cccatcgtgg aggaaggacc gtttgaagtt
    42061 cttatccggc ggggcgcacc acgaacagcg atgacggacc ttcccgggga tatgccggcg
    42121 atcatggcgg tgtttttctg gagggttgcg cgatggtcga accatgatcc ttcatccgtc
    42181 ggtcggagaa tgaatgaggc ggggagacgt gggatcgggg cggaaaatga aggggggagg
    42241 gcaggggccg tcaaggctcc cgtcctcccc cgcaggtggg tccagatatt cggaaacgac
    42301 aggttaaagc ggtgttgacc ttctccaaga accgggctct tgcatgatct ctacgaccaa
    42361 ggcttttcgg ccgccgcgtt ccggccggcg atgcggccga agacgacaca gtcagccatt
    42421 gccacgctgc ccagtcgcac ggcgccgtgc acgccgccgg tgacctctcc ggccgcgtag
    42481 agtcccggaa caggcttcat gttgaagccg gtaacctgag cgttcgtatc gatcaccagt
    42541 ccccccatcg tgtggtgcac ccgcggccag agccgggcgg cgtaaaacgg cggcatcacg
    42601 gtcggttttg cgtcgggaaa aatcatgcag tcgaactggg gatctttctt cttttcgaca
    42661 tacccgttcc agcgggcgac ttcctccaag agtgccgccc gggggatatt gtaatgctgc
    42721 gccagggtct ccaggctgtc aaatttcctg atggcgcccg aagccagccc cttctccagg
    42781 gcgttggaaa agacctgttt ggggacggcg taggcatcac cgaagatcag gaccggatgc
    42841 ccgaggagga tgatggcgtc cgcccgcacc ttccggtcgc ctgtttcctt gataaagcgc
    42901 ttgcccgtag cgggatcgac catgggggcg tagccgacga gccgttcgca gaaaagaggg
    42961 acgagaccga agcctttctc gtccgggctg gtccatgggc cgagctggat ccagtccatc
    43021 tgtacgtcca tggcccccac ttggcaggcc gcgagcaggg cttctccggt cgcgccgggt
    43081 tggttggtgg aatcgaattt gtcgttcaga cggggatcgt gcacctggcg cagcttgacg
    43141 tcctgggaaa aaccgcccga gccgaggacg acggcccttc tggcctttaa aaaggcggcc
    43201 ttccccgacg cttcattcgg aaaccggtag cccttgcgca cctcgattcc gacgatccgg
    43261 cccgcatcgt tgaggaccag gcggacgagc ttggtccggg tctggacacc gatcccgagc
    43321 tccttggcct tggccagcat cttgttcacc agttccgacc cagaggcgtt gacggtctga
    43381 acggcccgct tgaccgagtg gccgccgtga aagttgagcc gtgcaaattg ggcgcccacg
    43441 tactgctgac accattcgag ggcggcgttg gagccatcgg cgaccagctt ggccagttcc
    43501 ggatggttca ggtacgaccc cgccttcagc atgtccttca gcatgagttc ggcggaatcc
    43561 tggaccccct gttccttctg aagcttgttt cccgccgccg cgaaatcccc gccgttgatg
    43621 atcgaattgc ccccggggac gaccatcttt tcgatgaccg tgaccagggc gcccccgttg
    43681 cgcgcctcga tggcggcggt gaggccggcg aacccgctgc cgatcacgat gacgtcatag
    43741 gtttcgtccc attttttggg cattttgccc ggcgccgcca gcgcctggcc gcccagcccc
    43801 accccgccca cggccatgcc ggcgacggcc gcggcgcctc ccacggtttt catgaactgc
    43861 cgacgagata cgcttgccgc ccttttcgat gcttcgctca tggttgacct ccgtctgtgt
    43921 ttatgagggt tcgcattcgg gttccgctgt ttgcacaagg ggaaggatcc atccccacca
    43981 tacggtttat ctgtaaggga cgccccggtg tttgtcaacc cgaatatcgg gcgtcagacc
    44041 gcggcccgac ggtgcgccgc ttgcctgccc cgcttccccg gcggcgccta gcgccgaatg
    44101 gcttccggta ggatgtagac gtagggttcc cgcaggacgt gccgatgcag gagccggtca
    44161 atttttctga aggcgaggta gacccgccag atcatggcgt cttgccggta gtaggccgag
    44221 acctcctccg cggtgagtgg ggcgatgccg aacgccgccc ctttgcccgc aaagaagccg
    44281 ttggccgttt ccaggagcag ggggatgagc tccgggcgct gctccttgta gacattggcg
    44341 atcaggtcga tcgtaacctt gcggaaatcg tagtagcggg tcatgacgtc ttcgaggaac
    44401 aggagccgga tcagccagac gaggaaagag ggcgtacagc ggaggaacag ctcgggatcg
    44461 agctgttcga caccgtcccg gcggatcagc ggggtgccgg tgtcgaggta ccagagctcc
    44521 gacgcctccg tgatggggcc gtccgaccgg ccgtgcttca gcgcccagtt cgagatctgg
    44581 ccgtcgatcc cgaagatcag ggaggggtgg acccgatggc tcgcgaacac cttcgccagt
    44641 tcgcggagca cgcacccgaa aagaaggccg atggatcctt ccgcgagcgt gtggagcagg
    44701 gcgttgccga tggatgccga gggaagccga acctgcgccg tgtatacgat ccggttcccc
    44761 cgcttgggga cgacccgcgc cgaggcccat tccggcacct gaaggccgac ccggcccagg
    44821 cccgtattgt aggcctcgaa caggcgctcg taggcgtcca tctccgcgtt cgtgcggaag
    44881 atgggcatgc gcttgtaggc gatgtgcgcc tgtccgggat ggtcgatgac gaagatggtg
    44941 ctcatctcgc cgtagccgat gatcctggcg gaaatggcgc tcagatgggg attccgggga
    45001 tccagccccg cctcgaaatg gttcaggaga tcggggtcga tgacgtcttt cagcatatcg
    45061 ccagcacgcg cttggtttcg gccaaggcca gatcgagccg ttcgagaatc tcctgggcga
    45121 cggcccggtc gatcgtcagg ggcggcagga gttgggtgac cttccggttg ttgttcgcgt
    45181 agatgcagag caggccatgg tcataggcga ctttcgtgag caccgggccg cagaggtcgt
    45241 tgaccatctc gacgcccatc atcaggccga gctgcctcag accgacgacc agttccgggt
    45301 gccgggcgcg gagggattca aagcccgtcc ggaagaagcc ggccagttcc cggacatgct
    45361 cgagaaaagg cggggcggac gtaatctcca gcacctccag ggcgacgggg caaccgacct
    45421 ccgcgccgcc gaacgtggac acgtggatga acgggttctc atgaaaaaat gactgcaaat
    45481 ccgccctcag gcaggttgcg cttatcgggt atacaccccc ggaaagcccc ttgccgatga
    45541 ccaggatgtc gggcacgaca tcgaaatgtt cgatgcccca gagcttcccc gtacgaccga
    45601 gtcccgtttg gacctcatcg atgatgagga gcgcccctac ccgttcgcac agcgccttgg
    45661 ccgtccggaa aaaccccaag tcgggaacgg gcatgccgca ggtggcgggg atggtttcga
    45721 agatgacggc ggcggtcgtt gcatcgagcg ccccggccag ggcctccggg tcgtcgaagg
    45781 ggacctgcat gaagcccggg aggttgggac cgaagggggc gcggtaggcc tcgtcccccg
    45841 ttgccagcgc aagccctgta tggccgtgat atccgcccgc cgcggagacg atcttcgtcc
    45901 gaccggtggc cccccgggcc agcttgaggg ccaggtcgac cgcttcaccc cctcccacgc
    45961 cgaagacggt gtacgaaaga tctcccgggg tcagggaggc caggcgctcc gccagcctcg
    46021 cccgctgctc gctcaggagg tgatggttgc cgatgtccag ttcttccagc gccgcgatca
    46081 gggtccggat gacccgggga tgccgatgac ccaggttgaa gacgccgccg ttgcagtggc
    46141 agttgatgag ccgcttttcg cccgcggcgt cccagacgta tggaccctca cgccgcccga
    46201 agacgaagtc gatgccgacg ttcttgaaga attcggcttt gccggaggag acatggaggg
    46261 aatatcgggc gacgatgtct tccttggaat cctgaccgat gtgcttcatg gctaaagtcc
    46321 caggtaggtt tttcggatga cgtcctccgc gcgatcctcg ttgctgcggc gtgacgccgc
    46381 atttaccgcc gacccccttg aagccgctta aagatcaaaa gacaaaagaa cgcgctgccg
    46441 gactcgtgga acggtttgtg tcttgtgaac ctgtaattgt tgaagaaggg gatgtgttcg
    46501 gcggcccggg taccggataa agaccagccc cgccttttcc ccgagacggg gtttcgatcc
    46561 ttcgtccatc catgccgttc cctcgcggag acgttgacgg gtccaatcat ccgggagaaa
    46621 attaccccaa aaatctcatt gatcactagt ccgtttgccg gataaaagtc aagaggagat
    46681 tgatcgacgg agaaaatatc atcaaccttg ttgaagagac ttcatcacaa tcgaccgtcg
    46741 ggatgctgga agatcatttt tacggcgggt ttgatcggtt tgcccatggc attccggagc
    46801 aacgcctcga gggacaccgt ctccctgggg accttgtagg aggccaggcg ctgcttgagc
    46861 caaacgcgga gcgaatcggt atccaggcat cgtccggcct cgaggacgac ggcggcgcag
    46921 accctctccc cccagaccgg gtcgggtacg cccaccacgg cgcattcccg gatatccggg
    46981 tgggtccgca acgcttcttc gatctccagg gccgaaatct tttccccgcc ggtcttgatg
    47041 atatccacct ggctgcgccc caggatgcgg tacacgccgt tctcgcacac ggcgacgtct
    47101 ccggtcatga accagccgct ccggaatgcc ttccgggtct cctccggtct gcgccagtac
    47161 ccggagaaaa cggtccggcc ccggaccgcg atctcgcccg ggatgtccgg ggcaaccgcc
    47221 gctcccgcct cgtccgtgag ccgcacctcc acgcccggca acgggacgcc gacacagccg
    47281 gggaggcgct ttccgtgcag gggatttgac agcgccatcc cgatctccgt catcccgtac
    47341 cgctcgagga gaacatgacc agagacctca cgccacttct ccaacgtact cacgggcagg
    47401 gccgccgatc ccgacaccat gagccgcatc tttcggcagc cgtcggacat gtccttccgg
    47461 tcggcgggag acgaccgcac ccaggcgtcg atgagtttca cgtagatggt gggcaccgcc
    47521 atgaacagcg tcaatcggcc gcggcggatg cggtcccaga ccttccaggc gttgaacgcg
    47581 gggaggatct cgcacacggc gcccgcccaa agagcgcagg agacgacgtt cacgatgccg
    47641 tgaatgtgat ggagaggcag gaattccaga atccggtcgt tttccgtcca ttcccaagcc
    47701 ctgatcaggc tcgtgatctg ggccgcaagg gtgtcgtggg agatgaccac gcccttcggc
    47761 ctacccgtgg ttccgctcgt atagagaatc atggcctgcc gcgatgtcac caccgggggc
    47821 aatgcctcca aagggtgttc caggatgtcg gtgccgtggg cgaaacggag accccgatcc
    47881 cgggcaacgg ccagaagagg atcggaaacg tccggatggg cgatcaccgc tacgggagag
    47941 gcgtccccga tgacgtagtc cagctccggc ctcgggtgag agagagacag cgggaccgcg
    48001 atgcccccgg cccgccatat cccccattgt accgccacgt gctgaaagcc tggaggcgcg
    48061 agaaaggcga caggacgctc ctcaagatcc agcgatccgt tcagaagaaa ggaggcgacc
    48121 cgggcggatg cgtcgaggag ttgccggtag gtgaacgtgc cttccggagc gatgatggcg
    48181 gtccggtctc cgaaggagcc ggccctttcc gcgagcagga tcgacaccgc gcctcctcaa
    48241 agcgatacgg ggctcgtcct ggatcgacgg ggcaccccct catcttgctc gaacgcccct
    48301 ttcacttttc ctgaacgagg ggtatcaacg aaacgaatcg gtgtcgagca gacgagcgcg
    48361 gagagtatac agaatcggat ttttcgagtc aatgccggtg ttggcggaag caaaaaagac
    48421 agaagggcga ggcaccccgc tgcctgattt tgttatggtg ggcgatgcgc gactcgaacg
    48481 cgcggccttt ggttccggag accaacgctc tatccaactg agctaatcgc ccgtcacaac
    48541 gggagttgtc cttgccacgt tttatctcgc ttgtcaatca gaattcaggg acagtcaccc
    48601 aggctgcccg agtgacaaaa agttgaattc gtgcgctaaa cctgatagat aaacaacctt
    48661 atgaaggcat ttaatcccat cggcaacggc gtggggctta tcgcgctcgt cctcctgctg
    48721 ctcttccccg ccgacgccgc ggcgctgaac gagattatca acatccggca ttgggtcgcc
    48781 cccgatcaca cgcgcatcgt catcgatacg cgggatgagc cgcagtacac ggtgaaagag
    48841 ttgcccgaga agatcattct cgagtttccg tccatggcgc cgcccgaggc aaagaagaag
    48901 tggattcaaa tcctgaataa gccgggaatc aagaaaattc tcctcagcat ccgtccgcaa
    48961 caggtcatcc gggtggaact ggtactcgcc gcgcacacgg agaccaccgt cttccccctg
    49021 aaggccatcg aggacaagcc gtaccgcgtc gtggtggata tcgaacttcc ggaggcggcg
    49081 aaggaggcga cgcaggcgcg cgaacaggtc cgtgtcctga aaaagaagcg gatcgtcgtc
    49141 atcgaccctg gacatggcgg tgaagcgccg ggggccgtcg gcaaacacaa gaccctcgaa
    49201 aaggatgtgg tgttggccat cagcaagaaa ttgcgcgaca cgttaaaccg ccgcaagggc
    49261 taccatgcct acctgacccg aacgggggac tactacgtcc ccttcaagaa acgaatgcgg
    49321 attgcgaagg aatacggcgc cgatcttttc atcagcgttc atgcggatgc ggaaaaaagc
    49381 cggcaggccc agggaatctc cgtttactgc ctttccatgg gcggtgcgag caacgaggcc
    49441 gcgcgtctgc tggcaaagaa agagaacctg gcggatgtca tcgggggtgt cgccaacggc
    49501 aacggcaacg acgaatccga tccgatcatc ctggacatgt ttcagaccca cacgatcaac
    49561 cggtcacggg ccttcggtgt cgccaccctt cagagcgtgc gggcagtcgg tccactgaag
    49621 ttcgataccg ttcaacaggc ccccttcctg gtcctgaagt ccccggagat tccctccatc
    49681 ctcgtcgaga cgggctacat ttcaaacccg aatgaggagc gtctgttgaa gaaaccctcc
    49741 taccagcggc gtatggccga ggcgctggcg cgggcgattg tcaaggttct ctcttcctcc
    49801 ggcgaggggg taccgacaga ggttgcatct tcgacagtgg gtgcgaacga cgaccaatcc
    49861 gcatcgaagg ggacggatgc gtcgatcgga ccgccaactt cggacaaggc ccccacggaa
    49921 cggatcgaga agggtccgct tgcggcgacg ggcaccctcg acccgtcggg cgccagcaca
    49981 accgccgcac tgccccagcc ccggcgggag ggcgaggcca aggccaggac caagaccgaa
    50041 cccaaggccg aggccaaggc attccggcgg catatatatg ttgtgcggag aggcgacacg
    50101 ctgaccgcca tcgccggccg ccacggcgtg acgctcgccc aactcaagga ggccaatgac
    50161 tggagccgcg ggcagcgcct gctggcgggg cagaagctca tcctgccggg ccgttcggcg
    50221 gaatccgacg tggtcccggc gccgcaggcg gccgcccggt cggaacgaaa ggggaaggcc
    50281 gcttccgttt ataaagtccg gaagggggaa gggctggagc agatcgcccg acgttttggc
    50341 accgacgtgg acacgctttg ccggctgaac aaccttcggc gggatcgtcc gctgtatgtg
    50401 gaccggttgc tggtcctgcc ccagccccgg cgggagggcg aggccaaggc caggaccaag
    50461 accgaaccca aggccgaggc caaggcattc cggcggcata tatatgttgt gcggagaggc
    50521 gacacgctga ccgccatcgc cggccgccac ggcgtgacgc tcgcccaact caaggaggcc
    50581 aatgactgga gccgcgggca gcgcctgctg gcggggcaga agctcatcct gccgggccgt
    50641 tcggcggaat ccgacgtggt cccggcgccg caggcggccg cccggtcgga acgaaagggg
    50701 aaggccgctt ccgtttataa agtccggaag ggggaagggc tggagcagat cgcccgacgt
    50761 tttggcaccg acgtggacac gctttgccgg ctgaacaacc ttcggcggga tcgtccgctg
    50821 tatgtggacc ggttgctggt cctgcccccg gcctccggtg ttataagggg ggaccgatga
    50881 agcccgtccc gttcatcttg gtggtggccg tattctggct gttcgctgcg ccgacggtcg
    50941 cgcagatgga acccttccgg gtcaaggcgg agctggcgcc gtcagccccc cgtctcgttc
    51001 atcagggcaa gatcgtgggg cgtaagctcg ttcagccgcc tgcggatgaa gccgggccgg
    51061 gcatcgggac gggtgtggaa atgccggcgg acgacggccc gcttttccgc tttcgtctgt
    51121 ccgatagcat cagcgtttac ctcagccagt acgagggcct aacaacgggg cgcaacctca
    51181 cccagccgtt cggggaaacg aacaccatga gtgtttggaa gattctaccg agcctctggc
    51241 agcagggggc gacacgcgat accctttcca cggcggccgg catgttcgag ccggaaatcg
    51301 ggctggggat cgcgttttga aaaagagcga cgcgcgggga atcggttcaa tcccgaattg
    51361 gccccgccgg accgggcgct tccggtcgcg acgagaaagg atcatgcaat ggatgtgtgc
    51421 aagggactcc acgccttcat ctggcaggac taccgggaaa ataactgcaa caccttcctg
    51481 atcgacgggg agaaaaagat cctgatcgat cccggacacc gccacctctt ccagcacgtg
    51541 gagcggaacc tggcctcgct gaagctgaca ccggcggata tcgatgtggt gatcgtgacg
    51601 cacgggcatc cggatcatct tgaggccgcg gaactcttct ccaagccgac cctggtgacg
    51661 atgaacgagg cggaataccg tctggtcaaa tcgtggtccg gatcgtattt cgagggtccc
    51721 gatccggatt tttttttgca ggaaggcgat ctgaggatcg gcgccgagga tttcgagatc
    51781 atccatacgc cgggacattc gccggggtcg atctgtcttt actggcccgc caggaaggcg
    51841 ctgttcgccg gtgacgtcgt ttttcgggaa ggaatcggac gtaccgatct gcccggcggg
    51901 aacggcagcc aactcaaggc gagcatcaag aagatcgccg tgctggaggt cgagtacctt
    51961 ctgaccgggc atggggacat cgtggtcggg cgcgaggcgg ttcgcgagaa cttccgcgcg
    52021 atcgaggcgt actggttcca gtacctatag cctggcggac agggcctccc gtttcttcag
    52081 aaaggccgct tcgagcttgg ccagggcgtc cggccgggcg ggatcgagcg atgggttcgg
    52141 aaccacggcc ccgccggtga tgccgtaatc cgccagggcc gcgcgggcgg catcccgccc
    52201 ctgccgatcc aggcgcttcc gcttgttccg gaaggagcgg accaggtcct tcaggggtga
    52261 cgccgggacg tcgagcagcc gttcaatcag ggcgagcgtt tccatgtttt cgccgtccag
    52321 tgagatacgc tcgatcagtg ccgcccgcat gaggttccgt acctcagggg attttccgga
    52381 cgcctcggca tacagggatt ccagcgtctc cggagaaagg aggccgtcag cgaacttctg
    52441 cagccagccc cgcacggtcc cggacgtttc cctgagccgg atttccgcct tttcctccgg
    52501 ggaaagttgc atgtgtttcg ttcgttccat gatcagatcc agggtgctct tgatttctgc
    52561 catcggattc tcccataggc gtgatgccga tctataccgt atttgaacgg gatgatcaac
    52621 tgccggaagc tttgaaaagg aggttgaaga tgagaactgc ctggagactc gcggtgctgt
    52681 tcgggctcgg catcctcgcc ctgccgtttc atcccggcgt cgcgaccgcc gaggtgacga
    52741 ccctgaaatt ggcgcatgtc gtttccgtcg acggcgccta tcatctgggc gcccaggagt
    52801 tcggccgtct ggttgcgctc aagacccagg ggcagatcct tatcgacatt tatccgagcg
    52861 gccggaaggg ggatggggag cgggagatcc tgtctgcgat gcagaagggt aaaatcgacc
    52921 tcgttgtgat ttccaccggt ccggtcggcg ccatcgttcc gaggatgcgg gtctgcgacc
    52981 tcccctatct ttttcgcaat accgctcacg ccgaccgcgt tctggacggc cgcatcgggg
    53041 ccatgctgat ggaagacctg agaggggcgg gcattcgcgg catcgccttc tgggagaacg
    53101 gcttccgtca tctgaccaac agccggcggg ccgtcgtcac accggaagat gtgcggggac
    53161 tgaccattcg gacgatggaa aacgaaatcc atcaggcgtt ctggcgccag ctgggggcga
    53221 aggcggttcc catgccctgg gggaaggtct atcgtgcctt gaaaaacaaa acggtggacg
    53281 gacaggaaaa tcccatcgcg atcgtccgca ccgagaagat ctacaaggtc aaccgttatc
    53341 tgacgctgac ccgccatgcc tattccccgg ccctattgat aatggatgca aaaaaattcg
    53401 acggcctggg tgccgagcag cagcgggcga tcctcgaagc gggacgcgaa gcggcctggt
    53461 gggagcgacg tttcgtccgg gaaaaggagg cagccatgct gggagagctc gaagccgccg
    53521 ggatggttct cgtcacgccg gatcgcctgc cgttcaaaca ggccgcggag tccgtttaca
    53581 aaacctgcga ggacgttgcc gtaagcgacc tcgtccgccg gatacggcgc gtgaagtgag
    53641 cgacaggagg atatgatggg ggatctggcg cggcttttat cgccggaggt gattgccgtg
    53701 atcggggcca gcgagcgtga gggaaccctg ggacggagaa tcctggagaa tcttctcggg
    53761 gcgactggca gaaggatcta tccggtgaat ccgggaaagg aacgcaccct tgggcgtgat
    53821 tgctgcccga ccgtgagaga cattcccgag ccggtcgaca tggccgtgat tgccacaccc
    53881 gcccctctgg ttccggccat cgtgaaggag tgcggccagg ccggcgtgca ggggctcgtc
    53941 atcatttccg cgggattccg ggaatccgga ccggatgggg agcgacggga agacgaaatc
    54001 cgggccctgc aacgccgctt cgggatgcgg atcatcggtc ctaactgcct gggggtgatc
    54061 cggccgggca ttgagctcaa cgccagcttc ctgaaggata cgccaccgcc gggaaaactg
    54121 gcctttcttg cgcacgccca cggattcggc cgctcccttc tggactgggc cttgagcgcc
    54181 catgtcggtt tcagcacggt ggtatcgttg ggatccgcca tcgacgtgga tctcggggac
    54241 ttgatcgatt atctcggcaa cgatgcccag actcgcagca ttatcctcta cctggaggac
    54301 cagctcggac aacctcggaa actggtcagc gcgattcgcg gcttcgcccg gaccaagccg
    54361 atcatcattc tcaaaccgcc gcttttggaa attggtgcgg aaaacggacg ctcccacacg
    54421 gcgatcctcg ccgatccgga ccgcgtctgc gatgcggtga tccgccggac cggtgcggtg
    54481 cgggtgaagg aagcgcagga tctgttcaac gccgccgccg ttctctgttc ggcctaccaa
    54541 ccgaagggac cacgtctggc tatcatgggc aacgcgaggg gcgtcggcct catggcggcc
    54601 aatcgcctcc tccgttccgg cggacagctg gcattgctca caccggagac gcagcgggga
    54661 ttggcggggg ttctccccgc tcacgtttcg cgggacaatc ccttgcctct gcagcgcaat
    54721 gccgatgtcg gtcggtatga acaggccctg tccctgttgc aggccgatcc gcaggtggac
    54781 ggcgtccttg tgatctatac gccccaggag gccgcgcggg acgaagaact ggccgaggtg
    54841 cttgtgccgt tggcgcagtc ggttcgcaaa cccgtgatca cgacctggct gggcggccag
    54901 ggggtccggc tggggcgaga ccggatgggg aaggccggcg ttcccgccta tgaaacggcg
    54961 gaagaggccg tccggaccta tctgtacatg tacgaatatg aacgcaacct cgaactactt
    55021 catgaaacgc cgtcggagct gtccatcgac gatgcgccac cgaagcatca cctgaaaacc
    55081 ctgatccgga acgtccgccg gaaaggcgtc tccgttctga cggaagacga cgccatgaag
    55141 ttcattcgga actacggcat ccccctggtg gacacgcagc ttgtggcctc ccccgaggag
    55201 gcggtggaga tcgcccgtcg cgtgggttat ccggttgtgc tcaagatcgt ttcgcccgac
    55261 gtcatctacc gtcaggatgt cgggggcgtc gtcaccggcg tcgattcgga cgagaagctg
    55321 cggaaagagt acggtaagct cctgcagcgt ttccgggcgc gggcgcctca cgcggaaatc
    55381 aggggcatcg ccgttcagaa gatggtggaa ctgatcgact acgaaatcat cttaggggcg
    55441 agacgggacc cgactttcgg cgcgatcatt ctgttcggca tggggggagt gggcgtcgag
    55501 atcttccgcg acttttcgct cggcttgccc cctttgaatc agagcctcgc ccgcaggttg
    55561 atggaagaca ccaacgttta ccggatgctt caggggtacc gggggaagac gcccgccgat
    55621 ttgcgtcaga tcgaaggtct catcgtcagc ttttcgaatc tcgtcgttga ttttccggag
    55681 atcggcgaga tcgacatcaa tcccgtcgcc gtcagtgacg ggattgcacg ggtcttggat
    55741 gcgaggatca ttctgacaga cgagcctctc ccggacgcgg gcgcgcccta tccccatctg
    55801 gtgatcacgc cctatcccac acgctacgtc gcgaactggt ccctctccga ccgaaccccg
    55861 atcgttctga ggcccatccg tcccgaagac gaacccctag agcaggatat gtttcagacg
    55921 ctctcggaag agtcgctgcg cggacggttt taccagacca tccggaccat cagccatgac
    55981 ctgcacgtca agttttgcaa tatcgattat gaacgggaga tgtcgatcgt ggccgaactt
    56041 cgcgaggggg aaagacggcg cctgatcgga atcggcagcc tgatctccga gaccgtcgcc
    56101 gctcgcgggg agtttgcgat cctcgttcac gacgacttcc agggtaaggg tctggcctac
    56161 aagctcctcg atacacttgt cggcatcgcc gtcgagaagg ggcttaagga gttctacgga
    56221 atcatcgagc gccggaaccg aaagatggtc agcctgtgcc gcaagatggg catggtcgaa
    56281 gaacctttgc cagacggtct catcagggtg cggcttgtcc tgtgatgttt actgcggcgg
    56341 gggcgcctcg gtctgggcgg gggcggaatc ctcccagaag ctccgccaaa ggggagtggc
    56401 cgatccccgg atcgagaaca ggcgccgcag ggtcatttcc cggtccagta tcatcccgac
    56461 ggtatcgtaa cgccagagcc gaccgtaggt gttcacgttg atgttggttc cggtcacgtc
    56521 gctcatgggg atgcccaggg tgatctggcc gccgcggtag aatttctttt cgtagatcag
    56581 gagcgtttcg ccgccgaggt cctcgacgac gaggggtgcc ccgcattgcg caagaatctc
    56641 cgcgagggtc gtcctcccct ccgtgagacc ggcggccgcc gacgggacat ccggaccgat
    56701 cgtgaccttt tccacccgga aggacaggca gccggtcacg atgacgagcg aagacagcag
    56761 ccaggcgagg cctttaaaac gggaggccat aagccgcatc tccggaccgc agggcactgc
    56821 cgcagtgggt cacgacgttc tgcggattga ggaaaaagac cacctgatcg tactgggtgt
    56881 catagttgac gagcgtgacg aagacgagcc agaggcccgt ccccttgctc agactgcggg
    56941 agtagacata ggcgttcccg cccgtcaatt tgacgacctg tgtggggggg ccgaagaggc
    57001 gggtgacatc atcggcggtg gatttccccg ggacaatctg tgtcagcgcg gcgggatcgt
    57061 acgggtgaaa gttcttgtcg cgtccgaaaa cggcgcatcc ggccaggacc aggcagagga
    57121 ctcccaaccc cagcatcccg accgcattcc gccatcgacc tgaatcttgc atattttcca
    57181 tcaccggcct ccttcatcat cggcttgaac ggtttttttc cttcagaacg atccagttgt
    57241 tctcgtaatc tacggccagt tcgaactcac cgagaaaatt catccccaga aggccgtcga
    57301 acggcccccg gaaaccgttg tcgccggaca tgacgacggc cggcacatga tcgcgcccga
    57361 cgtttccgac gtcaagcctg gggatgagga tggaccggcc ctgcaatgtt ccgccgattc
    57421 cctcgatgat tacggcctcg ggggaggaca gggccgaatg cgaaaggcgg cgggcgagcg
    57481 cctcggtgat ggtgatgacg ctcgcacccg tgtctaccac cagacgtgcc gggacgctcc
    57541 ggttgatcag ggcgtccaca acgatgatgc cgcccgaatt cttggtgaag gggattaccg
    57601 cctctccgcg gtgctcgtcc agtgagggtg cgggagcccc ttcgggttcc cgttgaggtt
    57661 cggaataggt tctgacggtg acggcgtttg gcgggctgga cgccgcggcg ggcggcgagt
    57721 ccgacaggtg tgtgacgccg tcgacgtccg tccagcggta aatatcggcc gcgacgatcc
    57781 cgatatttcg cgggagtcgt ggtggcaggt tcaggcagag cgcaatcaga aggatgcatg
    57841 caagaagaag cggggggcct gccgggcgtt tcatctcccg aatctccgtg ttccgagcgc
    57901 cagaagcgaa gggacagttt accccgagat cgccggcgcg gaatccgacc gcgcgctgcc
    57961 gcgggccatg gttccgtcta caggaccgga caagacggtc gtcctttgca gcccggggct
    58021 ccctttcggc cggcctcggg acgggggtga agcacgtgca acttcgggta aaaaagccgg
    58081 ggcggaacag gacccatacg ccttgcccgt cccggctttg gatgtgatct gtcagcgggg
    58141 tcaggccttc gcgacgaccg cgggtttctt gtaagcgacg gcgagcagga agccgacgat
    58201 cagcaggccg gccgccacat agagggcgat gcctttcaac tgggcaaaca cgagcggtcc
    58261 gaccacaccg gccaggcccc aggccgtcag catcatcccg tagaccttgc cgatgtacgc
    58321 cggcccgaag gaatcggcgg caaaggccgg catggtcgcg aatccgccgc cgtagcaggc
    58381 gagcaggtaa caggcgatga ttgtgaagat cagcttgtcg ccgatctggg gcagcaggat
    58441 gtacaggacg gcctgcgtga tgaacatgat gatgaagacg ttctttcggc cgatcgcgtc
    58501 agaaatccag gcccaaagga gacggccgag accgttgaag atcgaggcaa tggccagaat
    58561 ggtgccgccc gccagggcca gctcggcggc ggagatgtcg gcgagggtct tcttgatgac
    58621 gtcctgagcc aggggggaaa gttgggaaat gaggccgagt cccgcggtga tgttgaggaa
    58681 gagcatgagc cataacatcc accactgcgg tgtttttgcc gcgcgctcga aggtgaagga
    58741 atcggctgcg gtaaccccgc tcgatgctgc cggtttgaat ccggcgggga gccagccctg
    58801 gggggggttc ttgtaggcct gcgcggcccc ggtgacgagc accaggaaga tgaggcccca
    58861 gatgtagaag gtgttcgcga cgcccatgga aatgaccatc gccggagcga tcttgcccat
    58921 gaagaaggcc cccaggccga agcccatgac ggcgagaccc gtgacgaggc cgcgcttgtc
    58981 cgggaaccag cggatcaggg ttgcaatggg ggtgacgtag ccgaagccgt ttccgagtcc
    59041 ggcgatgaga ccgaagccga gatacagcag ccagatgctg ccgatctgat cggccagacc
    59101 cgccaagagc gtgccgacgc cgaagaggat gccgccgatc gtggcgacga atttaggtcc
    59161 ctttttgtcg accagcgttc caccgaaggc cgcggacaga ccgatgacaa agatgcagat
    59221 catgaaggtg acctgcgttt cggtttctcc ccagccgtgg gtagtcatga ggggtttctt
    59281 gaagacggac caggcataaa ccgtgcccaa agagagctgc atgacgatcg ccgcgatggc
    59341 gatccaccag cggctgcttt cgaggttctt ctcagacatt ttctagttcc tccatcattg
    59401 agtgtattgg cgtcccttac cgggaacggt tcacgccgcc tcgaccttga ccgcgcagac
    59461 cttgtattcg ggaatcttcc cgatggggtc gagcgcggcg tttgtcagca gattgacggc
    59521 aacctcccgg aagtggaaat tcatgaagat cgtgcccgcc ggggatttct ccgtcacctg
    59581 ggccttggct tccacttggc cgcggcgcga cgtgacccgc accctctggc catccgtgat
    59641 tccgtaagcc ttggcatcct gcggatggat ttcgatcaag ctttccggac agcgctccga
    59701 ggggccttcg gccaggcgag tcatcgtgcc tgtgtggtag tgatacagga cgcgacccgt
    59761 ggagaggatc agcgggtact cccggtcggg aagctccgcc ggtgggatgt actcgacggc
    59821 gtgaaagagg cccagccccc gggtgaactt ttccttgtgg aggaagcgcg ttcccgggtg
    59881 gtcctgggtc gggcagggcc actggaggcc ttcgcgatcg atcctctcat aggtgattcc
    59941 ggcatagctc ggggtgacct gggcgatctc cgccatgatc tcctccgcat cccggtagga
    60001 catgggatag cccatccggt ccgcgattcc ggcaatgatt tcccagtcgg ccagggcctg
    60061 tccgggggga tcgacggcct tgcggatccg ctggatgcgg cgctccgtat tggtgaaggt
    60121 tccgtccttt tccgcgaagg cggccgaggg caggacgaca tcggccagct gggccgtctc
    60181 gttcaggaag atgtcctgaa cgacgagaaa atcgaggtgc ttcagggcgg cctccacatg
    60241 cgtaaggtcc ggatcggaca gcatggggtt ttcgcccatg atgtacagcc cgcggacatc
    60301 gcctttgtgg gcggcgttca tgatttctac gaccgttaat cccgggcggg gcggaagcgt
    60361 gacaccccag gccttctcga atcttccgcg cgcctcgtcg ttggcgacgg gctggtaagc
    60421 gggaaggctc acgggaaggc cacccatgtc gcaggcgccc tggacgttgt tctggccgcg
    60481 gaggggattg acgcctccgc cctcgatgcc gatgtttccg cagagcatgg cgagattcgc
    60541 cacggatttg acgttgtccg tgccggtggt gtgctgggtg agccccatcg cgtacaggat
    60601 ggaagcgcgg ccggccccgg cgtacaggcg ggcggccttc ttcaggtctt ccgccgggat
    60661 gccggtgatg tcctccacat aggccggagt gtacttcttg acgacctttt tcagggcctc
    60721 gaagccctcc gtccgctccg tgacgtagtc ggttgcgtag agcttctcgg cgacgatgac
    60781 gttcatgagg ccgttcaaga ccgcgacgtc cgtgccgggt ttctggcgga gccagagatc
    60841 ggcatatttc gtgagcgaga tttcccgcgg gtcgatgacc aggagttttg cgccgtgccg
    60901 ccgaacggcc cgcttggctt tcgcaccgat cacgggatgg ttctccgtcg tgttcgtccc
    60961 gatcgccagg atcatctccg tatgttcgat ctcctcgatg gagttcgtca tggcgccact
    61021 tccgaatgcg gcggccagac cggccaccgt gacggagtgt cagagacggg cgcaatgatc
    61081 gacgctgttg gtgccgacca ccgcgcgcat gaatttctgg aagaggtaat tctcctcgtt
    61141 ggtgcagcgg gcggagcaca ggccggcgat gctgtccggg ccgtgctgct ttttcagggc
    61201 ctcgaacttc ttggagacca ggtccagggc ctcgtcccag gtggcctcgg tcagttcacc
    61261 gccctttttc ttgcggatca ggggggttgt cagccggctc ggatggtgga tgaagtccat
    61321 cccgaagcga cccttgacgc acaggctgcc ctggttgggc tgtccgaact cacggtttcc
    61381 ggtgaccttg atcacccgtc cgtcatggac gttgagctcg atctggcagc caacgccgca
    61441 gtaggcgcaa gtggtacgga ccttcttcat ctcccaaggg cggccggaga agcgcgcctg
    61501 cttgaacgtg atggcacccg tcgggcagac gttgacgcac tcgccgcaga acacgcattc
    61561 ggaatcgatg tagtcggcgt cgaaggccgg tccgaccttg ctgtgcgacc cgcgcatgga
    61621 gaaatcgagg acctcgttca cctggatttc gttgcaggcg cggatacagc gaccgcaaag
    61681 cacacatttg ttgaggtccc gctggatcat ggtgttggtg gtttccaccg gatgacccgg
    61741 cgactgaatg gcgaaacggg gatggtcgat cttcagccag taaacgaggt cctggagttc
    61801 gcaggcgccg ttctgttcgc aggtgaggca gttgtggtct ccggatgacc agaggagctc
    61861 cacgatcatg cgctgggacg cccggacttt gggggtatcg gtccggacga ccatgccgga
    61921 actgatgggc atgcagcagg aagccacgag cgaacgggca ttttcgacct cgaccacaca
    61981 gacgcggcag gcgccgacgt tggtggtgtc ctttaagtgg cagagggtcg gaatgaaaat
    62041 gccgttttcg cgggcgactt caaggatggt ctggccgggt ttgccgatca gatcccggcc
    62101 atcgatattc aggggaacac tgctcatctc tatcctcctt ccggtccttg tcaaaagaac
    62161 ccggtcaaat ccgatggttc aaaagccgac tgcgtcaagg gcgcgggcgg cggcgggtcg
    62221 cgctcagatc tcgatatcgc agcgcagaca gcgacaggcc tccgcgatgg ccgcctcacg
    62281 ggtgaagccg agctccacct cagcaaagcc ttgacgccgc tcggagggcg ccatttccgg
    62341 catggccgtc tggggagact ccagagtctc ctcgtcgatg acgagcggga tgtcgatctt
    62401 ttcttcttcg ggcgcctccc aggccggctc acccgcggac tgacgaatcg acgcgtcaat
    62461 gtcccgggcc gcctggtgtc ccgcggcgat cgcggcgatg acggtatccg ggccggtaac
    62521 ggcatcgccg ccggcgtgga acttggggtt cgtcgtctgg gatttactgt cggggacaag
    62581 atccaagcag gcccaccggt tgacgctcac gccgctgtcc tcgggaacaa aggacgtgtc
    62641 ggggacctgg ccgatggcgg caacgatcgc atcgacgctc agggtgaatt cggatccttc
    62701 cacgggaatg ggtcgtttcc gcccgctgcg gtcgaattcg ccaagcatca tccgttggca
    62761 ggcgatggcg ctgaccttgc cgtctttccc ttggatcttg aggggggcca ccaggtactc
    62821 gatcttgatg ccttcatgta ccgctgcgag aatctcctct tcggcggcgg gcatgtcctt
    62881 gcgctcgcga cggtagagga tcgtcacctc cgcgcccagg cgcgcggcga cgcgggccgc
    62941 gtcgatggcg gagtttccgc cgccaacaac ggcaactttt gcgccaaggg ttttcttgcc
    63001 cgagagccag gcctcttcgt tggcgttgaa gtcccgcaga aattccacgc cgccccacac
    63061 cccgcccaag tcttcgccgg ggatgtccat cttctggctg gcgtgagcgc cgggggcgag
    63121 gtagatgacg tcatgctccg ccgcaacctt ccgccaggga aggtctttcc ccacgcgggt
    63181 gttgcattgg atgtccacgc ccagggcgcg cacatcgtcg atttcgcggg ccaggatatt
    63241 ccggggcaga cggtaggcgg gaatcgccca gcggagcatg ccgcccgctt ccggaagttc
    63301 ttcgaacacg tttaccttgt agccccggag ggcgagatcc ctggcggccg tgaggccggc
    63361 cggaccggcg ccgaccacgg cgattttttg cttccggtcg accggaaccg ccgtgatctt
    63421 cggccgaggg gcgttgtccg tgatgaagcg cttgaggaac ttgatcgcca ccggctcgtc
    63481 catcttgccg cgccggcact tggactggca cttgtggtcg cagacccggc cgcagacgga
    63541 cgggaaggga ttcgtcttga ggagtacccg gtaggcgtcc tcgaagcggt cagcccggac
    63601 cagggcaatg taggccggga tgtccatttc gatggggcag gtgtgctggc agggcgattt
    63661 gaacagcgcc gaacaaaccg ccgccgcgca gtggtggttc ttgatgtggt cctcgtactc
    63721 gtggcggaaa tagcggagcg tgctgagcac ggggttgggc gccgtctggc cgaggccgca
    63781 cagggcggag tccttgatga tccccgccat ttcctcgagc agggggatgt cgccctcctt
    63841 tccctggccc tgggtgatgt tcgtcaggat ttcgagcatc cgtttcgtgc cctcccggca
    63901 aggggtgcac tttccgcagg actcgtcttg gcagaagtcc atgaaaaacc gggccatgtc
    63961 cacggcgcag gagtcctcgt tcatgacgat gagtccgccg gagcccatga tggcgccgag
    64021 ctcggccact ttctcgtaat ccacaggcgc gttcaggtgt tgggtgggga tcatcccgcc
    64081 cgacggtccg cccagctgcg cggccttgaa tttcttgccc ttggggatgc cgccgccgat
    64141 gtcgaagacg atcttgccca agggagtgcc catggggacc tcgacgaggc cgacgttgtt
    64201 cacgtcgccg gtcagagcga agaccttcgt ccccttgctt ttctcggagc cgacgctggc
    64261 gtgccaggcg gcgccccgca ggatgatctg gccgatgttg gcgagggtct cgacgttgtt
    64321 caggacggac ggacgctgcc acaaacccgc gacggcgggg aagggcggac gcggtctcgg
    64381 catgccgcgc ttcccctcga tggaggtcat gagggccgtc tcctcgccgc agacgaaggc
    64441 cccggcgccc tggtagatct ccagatcgaa atcgaacccg gtccccagga tgtccttgcc
    64501 cagaaggccg gcttccttgg cctgtgtgat ggcgatgttc aggcggtgga tggcgagcgg
    64561 gtattcggcg cggcagtaga tgtaaccgtg atgggcgccg atggccttgg cggcgatggc
    64621 catgccctcc aggacggcgt ggggatcggc ctcgagcacg ctccggtcca tgaaggcccc
    64681 ggggtcgccc tcgtcggcgt tgcagaggac gtacttgatg tctcccggcg actgcgaggc
    64741 aaatttccat ttgaggcccg tcggaaatcc ggcgccgccg cgcccgcgca ggccggagtc
    64801 caggatctcc ttgacgatgt cctcggaaga catttcggtc agggccttgg ccatgccggc
    64861 atacccgtcg cgggcgatgt actcctcgat cttttccgga tcgatgatcc cgcggttccg
    64921 gagaacccga agctcctgca gggcgaagaa cgggatctcc tgcatggtgg ggatggtttc
    64981 gccggtcgcc ggttccttat acagcaggcg ctccaacacg cggcccttca cgaggtgctc
    65041 ctcgacgagc tccggaatgt ccgcggcctg gatcagggtg tacatgacgc cgtcgggata
    65101 gacgaccatg atcggtccca tcgcgcaaaa gccgttgcag ccggtctcga cgaccttgat
    65161 ctcgtcggcc agacctctct tatccagctc cgcgaggagg gccttcttca cctcgagact
    65221 gcccgacgca tggcagcccg ttcctccgca gagcagtaaa tgtgagcgaa acaccttcat
    65281 tatggatttc ctcctctata ttcgagaatc tgacatatca cggccggagg cggcgccaac
    65341 cgccccgggt tatttcacga tctcggcgcc ccgggcgaga acgaacgggg tctggacctc
    65401 cccctcgagg acgtggcgct tgaagatctg ccgcatcttg ttggggttca catattcgta
    65461 tttgatgggc tcccggccga tgatctcgac cgtgacgagc ggttcccggc tgcacagccc
    65521 catgcacccc gacgtcgtga tgaccacatc ggatacgccg gattcttcga tctcgcgcat
    65581 cagggcgtcg agaacctcgc gggcccccga ggcgatgccg caggttccca tgtggacggt
    65641 gacgcgcacg cggcggtctc cctcacgcag ggccgtctcc gcatggacct tttccttgat
    65701 cttcttcaag tccgaaattt tcagctttgc catcgctccc actcctcaat agggtttaga
    65761 cgtaagggcc gaggatgtcc ttgacctttg ccggtttgac gcgcccgtgg acgtcgtcgt
    65821 ccaccacgac caccggaccc aggccgcagg cccccaggca gcgcaccgtc tcataggtga
    65881 accgccggtc cggcgttgtt tcgccctcct tgattccaaa ggttttttcc agggtttccg
    65941 cgatggcctt gccgccgcgg acatagcagg ccgtcccgag gcagacccgc accgtatggc
    66001 ggccgcgggg ggtcatggtg aagaacgaat agaaggtgac cacaccgtaa acgcggctga
    66061 agggcaggtt cagcccccgg gcgatccgcg cctggacgga tggcggcagg tattccaggg
    66121 tcacctgggc ctcctcgaga accgggatca acccccccgg cttgccctta tgcttggcga
    66181 tgatggcatc gagctgagca atctgttccg gggtaaactc tttcgacaaa tcatcaagtt
    66241 ccgctttcat gcacccatat cctccttcta taggatcggt ggaagtcatc cttccgagcc
    66301 gatttcttca aggccttcca cgatgttgtc gcggatcagc ttgagcacgt ctctgtggct
    66361 catggggaca ccgtcgagtt cctgacggat ctcgcgcgta tccagaacat actccgccgc
    66421 gtcgtgacga tggcggtaaa cgaaatccac gtcgggattt cccgcgatca acgaaaccag
    66481 ggacgcggcg acatctccca gcggctgccg gtccagatga cacagaccga acgaagcctc
    66541 caggcgagtt ccccgacctg gcgcggagtc gagggtgaat gtgccgcccg tccgctgcgc
    66601 cgcatcggcc agcatcggca gccccagacc cacgcgccgg accttcttcg tggtgaagaa
    66661 ggggtcgagt gcgcgccccc gctcctcggc cgacatgccc cgaccgtcgt ccgcgacttc
    66721 cagcgtaagc cgatcgctat gcgtatcctc cgtcagcgtg atccggaccg ttttcgatcc
    66781 ggcccgggtg gcgttttcgg cgatatcgag gatgtggtga gagagttcca gcaaaaatct
    66841 actccagaat ctcacgtccg tcccgccgct gaaaggccag cttgagttcc gccaacgtgg
    66901 gggccgccat ccggatccgg gtcatcccct ggccgacgtc ttgcaggaaa tgcgcatccg
    66961 acgatgtgac gaaggcgtac gcgccgaact ccggataacg acggcgggcc tcccggatcc
    67021 ccaaccggaa cgtcacttcc agggcgtcga aggcgaggga cgaatcgatg aagcccagct
    67081 gacccagaac gccgaaagcc tcccggtcga tatgggaggc aaccgccagc ccgccgtggt
    67141 ggtgaatcag ggagatcaac ccgtccagcg ggatgtctgt ggccccgatg agcaggcgat
    67201 cgttgaatcc ctccacttcg tcgacgtcgt tcacgatggc ctgacagccg aaaacctctt
    67261 cccggttcga gccgctcagg tgggcgtaaa cgtcaacctg aaatcgcgct aacggatcca
    67321 gggtatcaaa aagagccagg acgtgaacct cttcgcggct ggtgacctcc atcccaggga
    67381 ggaccaccac cgacgtcccc gcggcgcaac gcagcacgta ggggacattt tccgacgaat
    67441 tgtggtcgca gacggcgata acgtccaagt ccgttttcag cgccttcccc acgagggcgc
    67501 tggggtaaag atccaaatcc gcacagggtg acagacaggt atggatgtgg aggtcacagc
    67561 gaaactcctt gagcatgacg gccaacctgg ggcatctgtc ataaacaaaa aaaaggccat
    67621 gattcccatg gccttgtacg ttacgttgcg atttgtaccg ctccggtaat cagggcgtat
    67681 accttgccgg cgactgcaaa agccgactcc tcggcgagaa ggacgggtat cccttccgcc
    67741 tcggccttgg tcaaggtttc cggcgcgggt tcgctgccgt tgaccagaac gatcgcggcg
    67801 agatccttca ggctcgccac ggccacaatg ttggggtgca cctgacgggt gatccagaga
    67861 tccccttcat ggctgttgcc catcacgtcg ctcaggagat cccccacata gcctccccgg
    67921 acggggcgat ccagtcgatc ggcgccggtg cgcacctgga gctgaagcaa tcggaccagt
    67981 gtggaaagat cgatcggtga ggtctcagtc atcttcaatc ccttaaggtc gccacttgat
    68041 gatcatcttc agagacgttc ccttgccggg ctccgaggag atctggaaga tatccgaaaa
    68101 attccggatg ttggccaatc ccatgcccgc accgaacccc atttcgcgga ttttgtccgt
    68161 cgcggtggaa taaccctcct gcatggcgag ttcgatgtcc gcgattccca agccctcgtc
    68221 gatggtttcg atgacgacct gatcgcgggc cagtcggagg tggatgaccc ctttgcgggc
    68281 ataggatacg atgttgattt ccgattcata ggccaccagg gccgttttcc ggaccgcctc
    68341 gctcgggatg ccgaatcctt tcagaatggc cttgatatgg ctcgccacct tgccggcgtc
    68401 gttgaaattt cctccctgga cgttgaactg gttctcgtag atcaggggct catcagccat
    68461 ggccgtgcac ggaaccatcc accttcacca gacacccgcg gatcccggcc tggtacagcc
    68521 gacccgcggt ttcaaaaaga atgaagcggg tgaccaggat gggaatcccc agatctcccg
    68581 ccagttgcac cgtatccggg gaagggcgct ttccgcggac gatgatgatc gcagcgatat
    68641 cgagaacatc cgctgtgcgg atcacctgcg gattggtcaa tccggtcagg agcaggcttc
    68701 ccgattgcgc aaaggccagc acgtcgctca tcaaatcggc cccgaacgcc gttttgacct
    68761 ccatatccat ctgattctct ccgaccagaa cctcggcatc gagaagatcc tttatttcta
    68821 ggaggatcac ttttcccctc tttccagcga ttgacgcggc cggctacccc ccgtgggatg
    68881 cgatttattc tgaccgccgg tcatgaagaa ttatatttaa attcacagtc gtgcgattag
    68941 cacaagcctt gagtgatgtc aacagatttg tctccgggaa cggaaaaatg ctttgcgtct
    69001 ccgcgcgaga tgggttaaaa cccctccatg ttgccgcaaa tcgaggatta cctgaattat
    69061 cttgctatag aaaaaggggc ttcccgacat acgctggcgg cctatcggca cgatctgggc
    69121 cgctatcgcg tcttcctgtc cgagcggaac gggggggacg gcctgaccat cgaaccggag
    69181 cggctgctcg actacctgac cgacctgaaa cggtccgggc tggccgcgag ctcaatcaat
    69241 cgggggtttg cggccatccg agggttttac cggtacctcc tgcgagagag aatcatcaca
    69301 gagaatcctg tggcccacat cgagcgcgcc aagacggggc tccggctgcc ggacgtcctg
    69361 agtcgccagg agatggaact gctgctcgcc cggccgggaa cgaacggacc cgcggctctg
    69421 cgcgatacgg ccatcctgga gctgatgtac gccaccgggg tccgcgtaac ggagctgatc
    69481 ctgctgcgtc tcggcaacat ccaatggcag atcggctatc tcacggtcat gggcaagggg
    69541 agcaaggaac ggatcgtccc cgtcggccgt tccgcctacg aggccgcccg ggtttatgtg
    69601 gacgaggccc ggccgctatt tttgaagggg gcctcttccg acattctctt cctcacccgg
    69661 tggggaaagg cgttcacccg gcagggcctc tggaagctta tcgcgaccta cagccgccgc
    69721 gcggggcttg cgaagcgcgt tcatccccac acgttccggc attcctttgc ctcgcatctg
    69781 ctcgagggcg gcgccgatct ccgctccgtc cagatcatgc tgggacatgc cgacatatcg
    69841 accacacaga tctataccca catgacgcgg gagcggctga aggacatcca ccggcgattt
    69901 catccccgcg gatgaatgcc tgcggcagga accgctcagg cgcggattga cggttagggg
    69961 agattgcccg ggaccgagag gggcagggac atgaacagac agaccgacga acgacgccga
    70021 aaggaggtca tgcgttgatg cggcgtccgg atattccccg tggagaacgg tgggttgacg
    70081 ggaaaacgaa ggcccggcgg cgctggagcg ccgccgcggc cgggctgctc gtcccggttc
    70141 ttgtgctggg cggattctgg ttcggtttca tcatcaggga tccccaacat gatccgatct
    70201 tcggtttgaa gcgggtccga aatgccctgt ccttttatgc cctgggcggg ccgccccgcg
    70261 tttacggcct gaccggagag cttaacgggc gggatttccg cctgaatccg tcggagacgt
    70321 ttacggtcac gtaccgggat gagttcatcc ttcatggggt cgtgtccgat gacctgctgt
    70381 cccgcggcgt cagcgtcgat gtcggtggcg tcggcggcga gcgtgatcag gggcgcatcc
    70441 tgaggggggt ggacctggtc gatcgggcca tggcggagcg gggccgtacg tctggggcga
    70501 cagacctgcc cgccgagccg ataaggattt actaccgggg aacccccgtt gccaccatcc
    70561 ccgtccaggc ggtcgttaca ccccaggact ggctacgcca tgcccgcgcc agcgaaagcc
    70621 tgaaggaacg gatcgactat ctcgaaacgg cgctccggat gaacccgtcc gacgggaacg
    70681 tccgaaagat gctggggggg atctacctgc ggtccggtga ggtcgaacgg gccatcggcc
    70741 attacgcgga ggtggtccgg caaaatccga aagatctgcc cgccctgagg gaactcctca
    70801 aggcttatgc ggcggggaaa aacgacgccg cggtcatccg cacgggtgag aggcttctcg
    70861 ccctgtcccc cggagaagca gccgtttatg cgcacatggc gtttgcctgc ggtaacctgc
    70921 gacaatggtc cagggcggtc gacctctacc ggaaatctct ggcgctggac ccccatccgg
    70981 agatgcttta ttttagattg ggggaggcct atgaaatgac aaaggattac cctgcggcca
    71041 tcgaggccta ccgggccgtc ctgaagcgga cgccggaagc ggattacgcc tggatggcgt
    71101 ttgcgggggc taacctcaag gccggtaaga acgaggaggc catccgcgtg tatcgcgaga
    71161 tcatcaggcg ccagccgcgc aatgtcctgg cgcatgccaa tctcgcactg gccttgggaa
    71221 acaagggcct gccccgcgag gaggtggcgc acaaccgcaa ggccttggcc atcgatcccg
    71281 gaaacaccat cgtccgcttc aacctggcgg ctgcctatga aaaactcacg atggaccggg
    71341 aggcggcacg ggagtatcgg caggtcctga aactcaaacc ggaggatgcc gatgccctga
    71401 aggggctggc cgatctggag ttccgttccg gccgctatgc cgccgcggcc gcgttgtatg
    71461 aacgcctggc gcgggtgtcg ccccagaagg cggacgtcta cgcgcagctc ggcttcgcct
    71521 acgcggaagc gaagaattat ggcctggcgg ctgaaaacta tgaaaaggcc ctgcgctttg
    71581 gaaaacggga cacccagctt cggcggcaac tggcggacgc ctacgaaaag ctggggcgga
    71641 agaaggaggc catcgcccag tacgaacggg ccgcggtcgc ccggccgacc ggcgaggtcc
    71701 tcgatgtcct ggcggatcgc tacatggcgg aggggcgcta cgaggaggcg gtgcagacct
    71761 accgaaagct gattgccctg aatccccgga agtcgtcgaa tcacgaagcg ctggggtatg
    71821 cgtacgagcg gcaggggaag aacgccgccg ccgccgaagc ctaccagacc tctctgaagt
    71881 atgatcctga aaacgcggag gtcttctacc gcctgggact cctccacgaa aggaaccggg
    71941 cctacgagga ggcccttcag gccttcgaaa aggcctatga gctgagcccc gaacacgtcc
    72001 gggcggggaa caagattccc cggatgaaga tcctcctcct ggaaagaaaa atgggtgaaa
    72061 aacaatgaag acgaaacagg tcatggtggg gagcgtcgct ctgggggggg aagcccccct
    72121 ggccctgatc gccgggcctt gcgtccttga aacggaagcg aaagcgctgg agatcgcccg
    72181 ttatctcaag acgctcgccg gggcgctgga gatcggcctg atcttcaagg cctcctacga
    72241 caaggcgaac cggacctctc tcgggggatt ccgcgggccg ggtctcaagg agggtctgga
    72301 gattctggcc gccgtcaagc gggagtgcga tgttcccgtt ctgacggatg tccatcgttt
    72361 cgaggagatc ggccccgccg ccgcggtggc ggacgttctg caggtgccgg cctttctctg
    72421 ccgccagacg gactttgtca tggaggtggc gcgtcgcgcg g
//
LOCUS       DAOOLJ010000009        65807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_264_length_65807_cov_18.107236, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000009 DAOOLJ010000000
VERSION     DAOOLJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 65807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65807
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_264_length_65807_cov_18.107236"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLW16_03090"
     CDS             complement(<1..249)
                     /locus_tag="PLW16_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69924.1"
                     /translation="MPVIWKNTKWPGVRYYEHPTRKHGVGRDRYFTIRYQRDGRRVEE
                     GIGWTSELDPKDKKHWTAEKAAVLLAELKEAAKGLKRGP"
     gene            complement(592..1023)
                     /locus_tag="PLW16_03095"
     CDS             complement(592..1023)
                     /locus_tag="PLW16_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HPB69925.1"
                     /translation="MGEPVLGKMERTHKLLRELGIGAILTLTEDDLYGHRHRKAGFRH
                     HHEPIDDAGPPTAAGMDRALAFIEASLPVGVAVQCMEGRGRTGTVLCAWLARQESLET
                     EQAIRRIHELRHRTVLTPPQRLFLRSYLSADAAPSKTRPVL"
     gene            complement(1154..1687)
                     /locus_tag="PLW16_03100"
     CDS             complement(1154..1687)
                     /locus_tag="PLW16_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HPB69926.1"
                     /translation="MKKLSSCLLFVLALTIVFHVAFVYFLPDIIMNYVIYRIQNDFDI
                     PTNTVYHNEVVNSDTRKVVRPCPDLLYSGMAYDVSERPVRIMAPVPPSYWSASFFAIN
                     TDNFYVMNDRTVKGGYADVVLVKKNAPYPVPKNTTVVVAPSPRGVILFRTLIDSPSRI
                     PLLIGYQKKARAEALDP"
     gene            complement(1684..2259)
                     /locus_tag="PLW16_03105"
     CDS             complement(1684..2259)
                     /locus_tag="PLW16_03105"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HPB69927.1"
                     /translation="MKKILIIAGCLITALVLGAASAFLAIKYTGQRGWVTNGAWRTNP
                     SIGSENLDMYSKANVALISLMSLNRSEAVYYYAETDDNGAPLTAACVYRIEGGDIDAR
                     WWSVTVYGSDQYLIKNGENRFSFNRAILRDGRSSRYTVRLSSTSRIGNWIPTGDEKRL
                     YLFLRIYNPGSEVLKNPASVRLPRIIKEGCR"
     gene            complement(2293..3084)
                     /gene="pcaD"
                     /locus_tag="PLW16_03110"
     CDS             complement(2293..3084)
                     /gene="pcaD"
                     /locus_tag="PLW16_03110"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HPB69928.1"
                     /translation="MTRAQINGTMLNYRLDGPAGGETVMLSNSLAANLDMWKFQVPPL
                     TDAGYRVLRYDGRGHGQSASSPGPYSMEMLARDAVGLIDFLGLEKVHFCGISLGGMVG
                     QMLGAYHGNRLSSLILCATASYLPFPEIWDERIEATRRGGMAALVDATLDRWFTQDGR
                     RRLQREIDEIRTAILNTSVDGYCGCGWAIRGMDFREAIRNISARTLIIVGEQDQGTPV
                     SEAEFIGERIVSSVVRVIPGAAHLVNVEQADRFNAAVLEFLDQPS"
     gene            complement(3142..3786)
                     /locus_tag="PLW16_03115"
     CDS             complement(3142..3786)
                     /locus_tag="PLW16_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69929.1"
                     /translation="MIQRYLMAALAALFILAAAPARADDDPAARVVRDPTLSYHKAFR
                     VETTPETWNRILDHPYLMTQLWTQYRFQPAYRVTRSGADLRVVDPSGITGDLRPLPPS
                     DASRAFYGSGTFRHWAVPSFFSAEGVVAFECKPDGGGLAGEAKIFMRGSNGISRLAMR
                     LFSALLLSRIDRRFTSNLNDTKKIIHDIVHDPDGVRNRLAGTLRDDFDRMVAPP"
     gene            complement(3804..4883)
                     /locus_tag="PLW16_03120"
     CDS             complement(3804..4883)
                     /locus_tag="PLW16_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB69930.1"
                     /translation="MESAAGQSWTEQLKHPVNTLRRLERFIETTPGEREAIATLQTVW
                     GTTPHFAALMDPRDPACPIRRQVIPSLAERENRYGRPDFLAWVEQGAEATPACILKAY
                     PDRVVFLVSDRCASYCRHCFRRDLILCPGRRPRLEREEGLRWLREHTEIRDVLISGGD
                     PFLLVDAEIDHLVRKLREIPHITLIRIGTRTPVVLPQRITPGLMKILRGYHRVPIWIS
                     LQCNHPREITEPMARAVYDLLSCGVSVGNQAVLLRGINDDAETFRELHRRLLTIRVRP
                     YYLFYCDFAPGIDHFRTPLEKGAELIRDALQGQITGLAQPHYVVSTNIGKIPIQARER
                     RSRAGRSCRLRNAEGKTVTLPRIPD"
     gene            complement(4925..5089)
                     /locus_tag="PLW16_03125"
     CDS             complement(4925..5089)
                     /locus_tag="PLW16_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB69931.1"
                     /translation="MPTYDYRCEACNRKFSLTLSVSERDRGKIVCPKCKSRKVRQQIS
                     SFTAKTSRKS"
     gene            5362..5817
                     /locus_tag="PLW16_03130"
     CDS             5362..5817
                     /locus_tag="PLW16_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB69932.1"
                     /translation="MANILVVYHSQTGHTEAMARAVAEGAAEIEHARVTLKRAGEAGL
                     QDLLDADGIAVGTPENFGYMSGMVKDFFDRTFYAAQDKVFRKPYVVFISAGNDGRGAL
                     QAIERIALGYKFRKVYDAVIARGELTEDVLAQCRELGGVLSGGCEAGIY"
     gene            complement(5858..6511)
                     /locus_tag="PLW16_03135"
     CDS             complement(5858..6511)
                     /locus_tag="PLW16_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69933.1"
                     /translation="MTEATQQALSGLRDLSMLKWYVIPLMAIVFYVYTREIKEAKRTR
                     DWNAVLAGLTVFGADFFNETWNGWVMHLTQRSAFWTTPGDTALRTMVGWNIEIMFMFL
                     LLGIIYTHTLSESRKERILGLPETWFWAIGYSVFCVFIECVLNVGGHLVWEYPWWSLS
                     FKGIWLIFLVGYFQFFCYAILVIGRKTLRAKLTIVGLIYAVPVVMNVLALGVWGWTY"
     gene            complement(6800..7561)
                     /locus_tag="PLW16_03140"
     CDS             complement(6800..7561)
                     /locus_tag="PLW16_03140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPB69934.1"
                     /translation="MTPRIKKHYPPVEDMNAAERVGMVREVFATIPSHYDILNRIFSL
                     RRDVAWRRFAVGKMRFGSTRRFLDVATGTADLALEAARQHPDVQVTGLDFAREMMAVG
                     VAKFRKLRFAGRIRLIQGDALNLPFPDHTFDVAGCAFGIRNIPDRPRALREMTRVVRP
                     GGQVLILEMHFPQHRLLKPFYHVYLNLLLPRAAGLVSRNPAAYRYLGDSILHFPAPAA
                     FTGLMAEAGLVRIARHPLMFGICYLYLGWKPEAPV"
     gene            complement(7558..8211)
                     /locus_tag="PLW16_03145"
     CDS             complement(7558..8211)
                     /locus_tag="PLW16_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPB69935.1"
                     /translation="MDLLLTLIDIAIHLDQHLKTVVEMFGVWTYVIVFLVIFCETGLV
                     ITPILPGDSLLFGLGALVAIGALDMAWMFPLLCLASILGDSVNYAIGNYIGPRIFDRD
                     KIRFFKKEHLVRTHEFYERHGGKTIIIARFIPIIRTFAPFVAGIGSMTYPRFLAFNVI
                     GGIAWISLFLFGGYFFGNLPFVKQNFTLIVVAIIVLSILPGVFEYLRQRGMARRSGT"
     gene            8474..9370
                     /locus_tag="PLW16_03150"
     CDS             8474..9370
                     /locus_tag="PLW16_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69936.1"
                     /translation="MSEENYGPLTLIRGENRGRYPYCHSVFIPGAGILIDPSSDREQL
                     IRLRDEGAVKTVWLSHWHEDHLMHLDLFDDLPLWMAEEDAPPLADVEVFLDWYQIDRP
                     DERAYWRKILLKQFHFHPRRPARFMRDGEVIDLGGVTVEVLRTPGHSPGHLAFFFREP
                     KILFLGDYDLTPIGPWYGDLHSSIEDTLASIRRLRAIPAERCLSCHETGVFDGPVEAL
                     WDGYEGTVFERERRYLELLEEPKTLAEIMGAWVFYGRPKEPRDFYEFGERVHAGKHLD
                     WLKAKGLLVERDGKFMSPSSEL"
     gene            complement(9359..10588)
                     /locus_tag="PLW16_03155"
     CDS             complement(9359..10588)
                     /locus_tag="PLW16_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69937.1"
                     /translation="MNAWQIEHQCPQCGAPVLLDETDRLLNCKFCRTRLLMTAPEGFR
                     YLLPPPADATGRELLFLPYWRLKGASYDCRPSGIVSRFVDTSTLALDAAGLPRSLGVR
                     PQAMKLKFRAAGQAGRFLEPGVPCREVVRAAAAQAAGGDGLQEFIGEMVSLIHTPVYF
                     DRNALCDAILNRPLPLADDRTGALTALLDAPAHPTAAVRFLPAQCPRCGWDLGGERDS
                     LVLTCRNCQSAWAGRNDGWEEVAFRVLPTAAGQVFYLPFWRMQARLDGLEMRTFADLI
                     RFANLPRVVTPAFAAAPVHFWSPAFKINPAQFLRWARQLTAAQPDGEGAAQFPEASRH
                     PVTLPLSEAGESIRVTLANLTPNRRQALPLLKETRVTVEAARLVYHPFLIGRSELLHE
                     RLRLTLDRAALAYGSKL"
     gene            10930..11364
                     /gene="mscL"
                     /locus_tag="PLW16_03160"
     CDS             10930..11364
                     /gene="mscL"
                     /locus_tag="PLW16_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPB69938.1"
                     /translation="MRMLKEFKEFAMRGNVLDMAIGIIIGAAFGKIITSFVNDLLMPP
                     VGLMLGNVDFSNLFVNLSGQSYETLAAAKEAGAATINYGLFFNSVIDFVIVAFAIFLL
                     IRQVNRFKREPEKAATTKECPYCCSEIPVKAVRCAHCTAELK"
     gene            11611..13128
                     /locus_tag="PLW16_03165"
     CDS             11611..13128
                     /locus_tag="PLW16_03165"
                     /inference="COORDINATES: protein motif:HMM:NF017546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPB69939.1"
                     /translation="MKKIMFILLCAVAALFLAASAGAERGKSFSVTPFVGGYTFEGNE
                     DLEDSLIGGLRFGYNLTNNLALEGVLNYGDSEYNLTDEDVDLYGYRLEALYHFMPDSR
                     FVPFVAVGAGGRSLRFDDTDRGDRNYLAAAYGVGLKFFFTDSLALRADVRHVIPFNDV
                     YNNLEYTLGLTYLFGGGAAPAARAEAVVSDADGDGVIDSADECPNTPSRAKVDAVGCP
                     LDSDKDGVADYLDKCPGTPAGVKVDKDGCPLDSDKDGVTDDRDLCPNTLSGVVVDKDG
                     CPLDADKDGVADANDQCPNTPVGATVDRKGCPLDSDKDGVADYLDKCPNTPKGVKVDK
                     DGCPLDSDGDGVADYLDKCPNTEKGVKVEKDGCPVLVEQKQEAKAEAVVKKMAKEKEA
                     VSLLVEFDTAKAVVKKKYYNEVKSVADFMKEYPHSKATIEGHTDSVGNASFNKKLSQA
                     RADSVRKCLIEKFGIDASRLTAVGYGEEMPIESNATRDGRQKNRRVEAIIESTDK"
     gene            complement(13231..14733)
                     /locus_tag="PLW16_03170"
     CDS             complement(13231..14733)
                     /locus_tag="PLW16_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPB69940.1"
                     /translation="METFSDYDRYDGLGLAELIRKGEVSAREVCEAAILRIETLNPAL
                     NAVIQPMFDLGRRAVETGLPDGPFRGVPYLLKDLVAAYAGVPLTAGSRARRDFIPGRD
                     SELVRRFKQAGLVILGKTNCPEFGLLAVTEPELHGPTRNPWDTGRTPGGSSGGSASAV
                     AAGMTPLASGGDGGGSLRIPASHCGLFGLKPTRGRNPTGPLYGTLWQGAVVEHAITRS
                     VRDSAALLDATCGADAGAPYVIPPPKRPYLEEVSREPGKLKIAFNTRSPIGTEVHPEC
                     VRAVEEAARLLEDLGHAVEAAQPDLNGEALAKSYLTMYFGEMAADIQELASILDRKAT
                     RRDVESLTWTLGLMGRTFSAGHFVRSLREWDIAARQMGRFHETYDLYLTPTVAFPPVR
                     IGELAPKPAERTAMNVINALGLGKLLQLSGITDKLAIESLSKTPFTQLANFTGLPAMS
                     VPLHWTADDLPCGVQFIGRFGDEAALFRLAAQLEAARPWFNRRPPKARPA"
     gene            complement(14947..16065)
                     /locus_tag="PLW16_03175"
     CDS             complement(14947..16065)
                     /locus_tag="PLW16_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB69941.1"
                     /translation="MGTDNVVFLEVLEWFDESGKELVHRLPEQGSGEIKWGAQLTVRD
                     SQAAVFFYQGKAYDAIGPGRHTLKTANIPILTKVLSIPWAMTSPLRAEVYLVNMKVFP
                     NLTWGTRDPVAFRDRELGLIRLRAFGVFNLRVLDPVLLINSLVGTQGVYTQSDIEEYL
                     NRVIVARFNDFMGERLDTIFDLPARYNELAGDLSGLLQEDFRRFGLGLADLYINSITP
                     PPEVQKAIDDKSRLGIFDDLNKLLKMKAAMAMENASQTQGEAGAGLGMGMGFMMPAMF
                     ADAFKTAGGTPSAAPGTAPCPRCGQSVPAGARFCPSCGRPVPATHTCTGCGADLPADA
                     RFCPRCGQAASSETPKCHQCGAETPAGAKFCSRCGKPV"
     gene            16303..17721
                     /locus_tag="PLW16_03180"
     CDS             16303..17721
                     /locus_tag="PLW16_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB69942.1"
                     /translation="MKSKGSEYPRIYKGIALIADPIHQYAVFTVPAEDGGGEKTEKDL
                     IDSPWVQRLRRIYQLQSARWVYPSAEHTRFQHSLGTMHLAGEFGRFLYPSLREVCPNL
                     PSAPYVEELLRVAGLLHDVGHGPYGHFFDDHFLNAFSLTHEDLGQAIITRKLARLIRG
                     LRRSPTGTLAAREVLDPAQVAFLIRMPDGRDGRAPEWLTLLRQLFSGTYTVDNLDYVQ
                     RDAYMTGYSLDMVDIARLKFYTFFSREGLTLHQAGVSALSRFLNARLNLYANVYFHRT
                     TRALDLHLQEIFRETMDLLSPLNPLVHLEAYLGLDEWTLFQEVGRWPEAKDARRRWLG
                     REWRKLHRRRVKWKMAFSTEISVDQIQRGTRFIPGTDYEQRIREFLPASVKKLAFRVD
                     LATQDPRPLNPMAETGKRINIFNPSTGRTSPEPLQELYRFIPARVVHFRVFALDHDHD
                     DLLTAAAEAALNAIEGAATTNL"
     gene            17721..19775
                     /locus_tag="PLW16_03185"
     CDS             17721..19775
                     /locus_tag="PLW16_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPB69943.1"
                     /translation="MILPEETTDITLFVRTSGEEIARWDRRRIVEALIRETGSEAGVA
                     EEISREVERQIVASGIAVLTTSLIRELVDAKLVERGLEQARRMHARLGFPLYDVEQLI
                     LHPNRENANVPHGPEGTNLILAEGIKREYALHHVFSRGVADAHIAGDFHLHGLGAVDR
                     PYSACQTLEYLKAFGLQLPHALNAARPARHPEVLLAHMVRFSAALQGHFAGVIGWLGV
                     NHSFAPYLTGMTDGEVEHFAQMLIYEFSQLTSARGGQSIYTDMHLSWEAPAFLAGLPA
                     VGPGGKAAGGTCDAFAPEAQRFARGLMAVFRAGDATGKPFTFPRPVIHLSPRFFATPG
                     HEAFLELACDVAADKGNPCFVFDRGDTGHAGLPFCPEAAGEPWRMRAAALLNVTLNLP
                     RLAYRAGGDERRLFGLISELLALAVRAQVEKKAFLKRILGLAARGPLALLAMDRDGAP
                     YLDLDRAGGLIGMVGLNELVQIHTGGQLHASPAALAFGLKVVSRLKDEVEALGRARGI
                     PLALEQTPAETTAHRFARLDLRFHSPASGRIVKGSIARGDVYYTNSTHLYVGAAVTPP
                     ERIEMEGRFHPLIPSDAITHVFLGAVRPSGAALARLVETAFRQTACAQIAFTPEFSSC
                     AACGRTRRGLFDRCPDCGADAVEGITRITQYVTGISSWNKGKLAELKDRHRHPGFFPD
                     SE"
     gene            19908..20492
                     /locus_tag="PLW16_03190"
     CDS             19908..20492
                     /locus_tag="PLW16_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPB69944.1"
                     /translation="MEDHIIRSFKEGSELKEVFLNENLGHVVRAIEAITTALQAGNKV
                     LLFGNGGSAADAQHLAAEFVNRFMIERPPLPAIALSTDTSILTSIGNDYDFSEIFSKQ
                     IRALGQEGDVAWGISTSGKSANVVRGLETAKKKGLVTIGLTGGDGGDIARIVDYALIV
                     SSRNTPRVQEVHIAVGHVICEMIDYKLFQRPDAP"
     gene            20552..21121
                     /gene="grpE"
                     /locus_tag="PLW16_03195"
     CDS             20552..21121
                     /gene="grpE"
                     /locus_tag="PLW16_03195"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB69945.1"
                     /translation="MTAAFETSSMPEAGDGRPETDRTVGEDLDALRAKLHEKEQEAAE
                     NYDRYVRAVADLENYKKRALRDKADAVKYGNENVIRDLLPLTDSLDRALKQAEASCDF
                     EAFRKGLEMLRVQLLGSLEKHGVQAIDSLEKPFDPNVHEAMMQVDSDAHEDNQVVDEF
                     EKGYTLNGRLLRPARVSVCKKRTNEGVCE"
     gene            21161..23083
                     /gene="dnaK"
                     /locus_tag="PLW16_03200"
     CDS             21161..23083
                     /gene="dnaK"
                     /locus_tag="PLW16_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPB69946.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGDPAVIANQEGNRTTPSIVAFTE
                     SGERLVGQVAKRQAITNPENTVYAIKRMIGRKYTSKEVQYDKTISPYQITEASNGDAQ
                     VTVRGRNYSPAEISSMVLTKMKQTAEDYLGEKVTDAVITVPAYFNDSQRQATKDAGKV
                     AGLNVLRIINEPTAAALAYGLDKKKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGEDFDQRLIDYLATEFKKDQGIDIRSDRMALQRLKEAAEKAKMELSTSMETDV
                     NLPFITADASGPKHLNVKITRAKLEALVEELIEKLDAPCRTALKDAGLTPGQIDEVIL
                     VGGMTRMPRVQQKVKELFGKEPHKGVNPDEVVAVGAAIQGGVLSGDVKDVLLLDVTPL
                     SLGIETLGGVMTRLIEKNTTIPTRKSQIFSTAADNQPAVSIHVLQGERPMAADNRTLG
                     RFELVGIPPAPRGMPQIEVTFDIDANGIVHVAAKDLGTGKEQSIKITASSGLSEEEIQ
                     KLVREAEAHAGEDQKKKDLIEARNQADAMAYSVEKNIKEFGDKIGSDEKTRIEEAIAK
                     VKKTIEGDDLDAIKAAQEELTTASHKLAEAMYAQSTQQQAGTGPGAGAGADAGAQTGG
                     GKKDDDVVDADFEEVK"
     gene            23381..23926
                     /locus_tag="PLW16_03205"
     CDS             23381..23926
                     /locus_tag="PLW16_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPB69947.1"
                     /translation="MNKPLAAWIFAAVLAIVIAVAPFPADASIRLLKDREYVRDLIRA
                     IDGAREEIVVCMFLFKASGYRGSYPDRVVERLLKAARRGVRVEVVLERGGDPTGTVDR
                     DNEATRRRLEQGGVRTRMDSPRRTTHTKLVVIDRRTTFVGSHNLTHSALKMNHELSVV
                     IDSPAVAAETLKYIRTSVLRD"
     gene            23962..24723
                     /gene="surE"
                     /locus_tag="PLW16_03210"
     CDS             23962..24723
                     /gene="surE"
                     /locus_tag="PLW16_03210"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB69948.1"
                     /translation="MRYLLTNDDGIYARGLSALYQELSRDAECLIVAPEIEQSAVGHA
                     ITLLRPLMVRRAKREGEFYGYAVLGTPADCVKLGIRELVREPVDLVVSGINRGANVGI
                     NVLYSGTVSAATEAAILGVPAMAVSLDTHREADYTVAAKVARRLAAWMTNNGLGSGVA
                     LNVNVPALPEDRIRGIAVARQGRARLMESFERRVDPREHEYYWLAGETQLAVDEEPDS
                     DASALKQGLVTITPIDYDLTCHESLDRLKDLIAKI"
     gene            complement(24835..25674)
                     /locus_tag="PLW16_03215"
     CDS             complement(24835..25674)
                     /locus_tag="PLW16_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB69949.1"
                     /translation="MIRSILIPTDGSEQGKIALEYALYMAGKLGASLTGLHVVDIRLL
                     QAPVFSDISGSVGLPPYQEFLPAVEGGLEARAETILAAFQQRCGEAGIPATARKVMGV
                     IDESIIEAGREADGILLARRGEHFHLGSGGILGSTAEAVVRKAGKPVVVTPDTFREIE
                     SMALAYDGSAPARNALSIATFLSEKAGWPLTVVLITDNQTLAAERMKQVEEALADVKI
                     DAQTIVLPGKEDKALLRFIQEGSVELMVMGAYGQGRLRELFVGSTTTAVIRKSPIPVL
                     LTR"
     gene            complement(25684..26367)
                     /locus_tag="PLW16_03220"
     CDS             complement(25684..26367)
                     /locus_tag="PLW16_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPB69950.1"
                     /translation="MFAKRNTLHEKIYHDDEDRLKERIHILDKKWEGDYTSYFSNGQV
                     MAQMRFHQGRREGEAFSYYRDGRLREKGTFVNDRLEGDYVCYYENGQIRESEHYRNGA
                     LDGVYRLYYRNGQLKEEEHFRNGKFDGPYVSYYKSGQIKEQGAFVNDKRDGRYMSYWR
                     NGRIRETATYRNGRLVGKFVSYDEAGREIETVLPADTADTEENEDERMEKDKIIDDLL
                     KNFSTYEKL"
     gene            complement(26377..26868)
                     /locus_tag="PLW16_03225"
     CDS             complement(26377..26868)
                     /locus_tag="PLW16_03225"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPB69951.1"
                     /translation="MTSIRYGGFWRRFAAFAIDKALLYGVSLFLFLILVLAGGVDLPS
                     PRRSPTPDAIEALIRGMTGLYLFVSTLASAAYFTYFHGLTGQTPGKMAMKLQVIQISG
                     ERMTFGLAFLRWVGYILSGIFLYLGFLWIAWDGRKQGWHDKIAGTVVVRRPAPPTGTG
                     TDA"
     gene            complement(26865..27641)
                     /locus_tag="PLW16_03230"
     CDS             complement(26865..27641)
                     /locus_tag="PLW16_03230"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPB69952.1"
                     /translation="MTAPRIYYPHPFAVGETCVLDGNWRRYLRTVLRLREGDALVLFD
                     GQGREGRAHMTGIDRDGVSVQVGVCRVVPADALPLTLAQALPKGETMEAVIRKATELG
                     VTAIRPFPSERSIPRLTAEKARQRVARWQTVAVEACRQSRRADIPRVEDIASFEDIVA
                     APEDGAVRLMLWEEERETGLRNLLRGLTDLKGRPCTVVIGPEGGFTEAEVRLAKGHGF
                     LIASLGRRVLKVETAAVAALAIVQYERETLGTAEKGNDGT"
     gene            complement(27638..28591)
                     /gene="prmA"
                     /locus_tag="PLW16_03235"
     CDS             complement(27638..28591)
                     /gene="prmA"
                     /locus_tag="PLW16_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPB69953.1"
                     /translation="MPAKQGMTPDKRERWFKIELSTPPELVDALANYVTEIGAQGAYQ
                     EVLEPFAPNDRPAALDKLNAFLPVDTRLENRLARLETYLASLGKLFPEYDPVTLTTET
                     IDDPDWGEQWKKYFKPLRVSKNIVIKPTWERFTPQGHDIVIDIDPGMAFGTGQHPSTR
                     MCLEAMEDILLKNRSVRKARVLDVGTGTGILGIASAKLGAEAVVCVDIDRNAADIARE
                     NVRINGVEDRVKIVNRDVTTLHDPFSLIIANLTARLLIRLRSHLVSLLTPGGYLVLSG
                     IIDQNRPEMEAHFFAPPFVSRRTITEKEWVCYVLQNKEQTP"
     gene            complement(28785..30074)
                     /locus_tag="PLW16_03240"
     CDS             complement(28785..30074)
                     /locus_tag="PLW16_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB69954.1"
                     /translation="MKPVMPLLAAALALFSWSCATVAPTKAPPEPASVLYLEGTVQGP
                     AGNGLALAMKLPEMGKTPGSPVGEIAQALIRKSLIIEGMRTDVNGKPATVGEVRGATV
                     TVALDKPEPLPAGVPVRLTIPKKTIAIVDFDVIRGREKDQGRVTLEGLTTALIDSGQF
                     NVVERQKLKAVMNELTLSQSGLAREKPGDVLGKLMMADLLLTGTLAEVAGEWDIHLRL
                     VNVRTSQAMSAIHVKTALLKASEIRDSGPFDETFESPVRDPSWIMVATGRTAPFFRTE
                     LDRADGSADSGRSMRIDYRLVPGRNPEWARIDNRKKRDLSLYDGIAFDARATETVHAQ
                     VMVMTSMPDDPNRVDAWTGFFEAGPAWGRIRIPFAQMVVARGWIKQGAAAMYGARPGD
                     QVLRLERVEGFQIGLNQKMNGDVAGSLWVDGIRFYRD"
     gene            30309..30974
                     /locus_tag="PLW16_03245"
     CDS             30309..30974
                     /locus_tag="PLW16_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPB69955.1"
                     /translation="MNYYPICLNVAKKRCVVIGGGQVAARKVERLLDCGADVVVVSRA
                     LAPELADWKAEGRIVHRESEYHAACLDGAFLVIGATDREDVNAAVSRDARARGLLVNI
                     VDAPAHCNFILPSLHQQGDLVIAVSTGGKSPALARKMREEFARIYGWEYGVLVEILGE
                     LRERIMARGRPPDENKQRFDAVVGSDVLEAIRRRDAEAVRGLILRLTGEDLPVSFREG
                     AEP"
     gene            30974..31789
                     /gene="ccsA"
                     /locus_tag="PLW16_03250"
     CDS             30974..31789
                     /gene="ccsA"
                     /locus_tag="PLW16_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPB69956.1"
                     /translation="MDILLFRAALSLYLVSCLGYLSSLFARRVLVAKYAGWGLLAALV
                     VHTLAFGARCLATHQNPLVNIYAALSFFAWVLAAVYLVFQVRTKTRVLGVLVAPAVVI
                     LMMAASAGLGGAVALPPILRGHLVSVHVVLAVIGEACLALVGLAGAMYLLQDRLIRKK
                     RATRLLRLLPPLEDLDRINHLGLLCGFPLLTLGVIAGALWARTVWGTHWPGDPKLVWT
                     LLAWALYGLLLHQRLALGWKGRRMAIGALTAIGFLLAALAATGLFFQSVHTFG"
     gene            31794..33059
                     /gene="hemA"
                     /locus_tag="PLW16_03255"
     CDS             31794..33059
                     /gene="hemA"
                     /locus_tag="PLW16_03255"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPB69957.1"
                     /translation="MHLVLIGMNHRTAPLDIRERLSLSCGEEGRPLAELLAIPGVDEA
                     FYLNTCNRVEVLVGTGTPDAALAGLRDFVLRHGNLSGEMLERCLYLYRDEAAVRHLFR
                     VTASLDSLVMGEPQILGQVKDAYRSSAEANAAGAILNRVLHHAFRTAKRVRTETAIAA
                     NAVSVSFAAVELAKKIFGDLRGKTGLLVGAGEMSELAARHLLGQGVGRIVIANRTLAR
                     AQEMASALDGEAVGFERLPECLEAADIVIASTGSPGYVVTAEMVGRALHRRRNRLLFL
                     VDIAVPRDVEPAAARIDNVYLYNIDDLQGIVDANLAQRREEARKAEGIIDAEVANYLE
                     WFNTLEVVPTIVSLREKVDAILEGELERSHAWMQHMREEDRENIGILVHAVVNKILHD
                     PITGLKEESQDHAARPYVAALRRLFHLES"
     gene            33019..34020
                     /gene="hemC"
                     /locus_tag="PLW16_03260"
     CDS             33019..34020
                     /gene="hemC"
                     /locus_tag="PLW16_03260"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPB69958.1"
                     /translation="MWPRSGACFIWRAEKDPSKGGRTIRNLIRIGTRGSQLALTQTNA
                     VATEIRKRHPALTVEVQAIRTKGDIMQDVSLAKIGGKGLFVKEIEEALVRGEIDLAVH
                     SMKDVPVELPAGLAIGIVTRREDPRDVLVSRDRLKLEALPEGARLGTGSLRRGFQLRN
                     ILPDVEIVSLRGNLDTRIRRVGTEGLDGVILAAAGIRRMGWVERVSQFIPVEVLLPAI
                     GQGVLGIEHRSDDRELLEALAFLHDRETALAVTAERAFLADLGGGCQLPVAAYARLRG
                     PELVLRGLVGSLDGRAMIRDEVRGAADDGATLGRRLASTILDRGGRAILEAVYACGD"
     gene            34136..35653
                     /gene="cobA"
                     /locus_tag="PLW16_03265"
     CDS             34136..35653
                     /gene="cobA"
                     /locus_tag="PLW16_03265"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPB69959.1"
                     /translation="MDKIGKVYIIGAGPGDPELITAKAIRCLKEADVVIHDYLVSREI
                     LRHASTSARFIYAGKQGGDHTLSQEEINRRLVEEARRGHTVARLKGGDPFIFGRGGEE
                     AEELARAGIPFDIIPGVTSAIAVPAYAGIPLTHRGLTSTLAFVTGHEDPTKPASDIDW
                     TALAKIGSLVFLMGVKNLPEIAARLVEQGRDPATPAALIRWGTTPDQETLTGTLADIA
                     VKAHAEGFRPPAILVVGEVAGLRGRMNWFETKPLMGRGVVITRPEAQADEFAALLTAR
                     GARVLRFPTIAIAPPERWDALDAALARLATYAWIVFTSANGVRFFFHHLRETGGDIRD
                     LKGIRLATIGPATAATLEAMGLKVDLVPASFLSEGVVAAFAGQDVAGKAVLLPRAAEA
                     RDVIPEGLAKMGATCDVATVYRTVRSDRKKEELAAWLADGKVDVITFTSPSTLTHFRD
                     IMGPEFRLPPAVRIACIGPVTAAAVKCAGLPVDILQETYTIHGLVAALEAHFSSP"
     gene            35801..36985
                     /gene="ahbC"
                     /locus_tag="PLW16_03270"
     CDS             35801..36985
                     /gene="ahbC"
                     /locus_tag="PLW16_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HPB69960.1"
                     /translation="MIGISKLYCGAVEPGDVLRYGRESGKLPSHLLQFSADKKPVVVW
                     NVTRRCNLRCIHCYSSSRDVEYRDELSTAEGRALIDDLADFGAPVILFSGGEPLMRPD
                     LSELVHHAAARGVRAVISTNGTRITEAFAAQFRDIGLSYVGVSLDGLEPTHDRFRGRA
                     GAFAEAIRGIRICREAGIKVGVRFTMNRLNVRDIPAIFDLLEAEDIPRACFYHLVYSG
                     RGSKLMEEDLSHAESRAVLDLIMDRTRSLFDRGNPKEILTVDNHADGPYVYLRLLKEG
                     PRRAAEVLELLKMNEGNSSGNGIGCVSWDGEVHADQFWRGISFGNVRRRSFSEIWSDT
                     SNELMARLKDKRPHLTGRCATCRWLDVCGGNFRARAEAATGEIWAPDPACYLTDEEIA
                     RT"
     gene            37080..38063
                     /gene="hemB"
                     /locus_tag="PLW16_03275"
     CDS             37080..38063
                     /gene="hemB"
                     /locus_tag="PLW16_03275"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPB69961.1"
                     /translation="MNFPDYRPRRMRKNENLRRLIRETRLVADNLIYPLFVVPGKGVK
                     KPIASMPGVFQMSADVLVQEVREAREAGLPAVLLFGVPERKDEVASGAFARDGVVQQA
                     LRRIKDKVADILVVTDVCLCEYTSHGHCGMLKKGAVDNDVTLEVLAETAVSHARAGVD
                     MVAPSAMMDGQVGAIREGLDEAGFDDLPIMAYAAKYASAFYGPFREAAESAPQFGDRK
                     AYQMDPANGDEAIREITLDVEEGADVIMVKPALAYLDVIRRAREEFDLPLAAYNVSGE
                     FAMIKAAAQLGWLDGERAMWEVLTAIRRAGADMIITYFALEAARGLRKAQG"
     gene            38257..39456
                     /gene="ahbD"
                     /locus_tag="PLW16_03280"
     CDS             38257..39456
                     /gene="ahbD"
                     /locus_tag="PLW16_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HPB69962.1"
                     /translation="MADSVTQKNAPKGGETDARKARALPILPAVLRMVAWEITRSCNL
                     SCVHCRAAACRGPYPDELDTTRCLGLLDEIAAFARPVVILTGGEPLLRPDVFELAAYG
                     TALGLRMVLATNGTRVDDAVARRLIGAGIQRVSVSLDGLDAVSHDAFRQVPGAFDGAL
                     AGIAALREAGLEFQVNTTVTRENLSQLPGILDLAERLGAAAHHIFLLVPTGRGKDLAG
                     QEIDAEAYERTLEWFHEQSGACALQLKATCAPHYFRILHQRGGKVRASGASDGSRPGP
                     GPDGGAGKSAHPLHAATRGCLGGSAFCFISHTGQVQPCGYLEVDCGQVRERGFREIWE
                     TSRIFTDLRDLNRYGGKCGRCEFIRVCGGCRARAYEATGDYLAAEPLCAYEPKGARRP
                     DGRKEDA"
     gene            39453..39848
                     /locus_tag="PLW16_03285"
     CDS             39453..39848
                     /locus_tag="PLW16_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69963.1"
                     /translation="MKRIIWGCGLALLLVGAVSAAGPTFEQLDTDRNGKLELNELDAA
                     AVKLFRDADVNRDGVLDRHEFKRLQGPSSSFDDLDANRDEKIDLGEIRRSAALRFDRH
                     DLNQNGALDDMECSPNRMPTVSPLMSVYF"
     gene            39862..40305
                     /locus_tag="PLW16_03290"
     CDS             39862..40305
                     /locus_tag="PLW16_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HPB69964.1"
                     /translation="MDELDRKILNLIQTNFPLVPEPFAQVAEAVGSTEAEVLARVRDL
                     RERGIIRRLGAVFDKERLGFASVLCAARVPEERLREFVATVNAFPGVTHNYRRDHDYN
                     VWFTFIAPSEEALARALDAIEERTGVEILRLKATKTFKINASFAL"
     gene            40432..43116
                     /gene="polA"
                     /locus_tag="PLW16_03295"
     CDS             40432..43116
                     /gene="polA"
                     /locus_tag="PLW16_03295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPB69965.1"
                     /translation="MNDRNRPTLVLVDGSNYVFRAFFAIRDLTNSRGFPTNAVYGFTT
                     MLMRLLKEHRPDYIAVAFDVKGPTFRHEAFDAYKATRRPIPETLIPQIPVIRKVVRAF
                     NIPVLEKQGVEADDLIGTLAKRHAAQGIRVLIVSGDKDMMQLVSDDVVMVDTMRDKTF
                     DVAAVRERFGVAPDRVVEVLGLMGDASDNIPGVPGIGPKGAQRLLEQFGTIEAMLAQP
                     EKIYNARMREAVLAHAEQARLSRELAVIRTDVPVELDLESCRYTGPDAATLKALFQEY
                     EFTSLIKELELRDAPQAVVSRLVRSEADREALRGALRGAPEIALDVVLSVPEAMRAEW
                     VGLAVSTATDQAFYLPLIAPEDGEAPAQAAADILAAVAPVLTDPSVRKHVHDLKTALI
                     VLERNGIALAGIGCDTLVAAYLLNPGRRGFELAEIADEHLQRSLAAAKEIVGSGAKAV
                     SFATIPAETLADYAGVRSCAVRELAGMLMAELDRQGFRDLFADVEMPLTPVLADMERH
                     GVLLDVDLLRRMSQEIEQLMALSEEKIYRLAGERFNVNSPKQLQVILFERLGLPKGRR
                     TKEGYSTDVDVLNFLARSHELPAEILAYRSMAKLKSTYIDALPQLVHPETGRVHTSYN
                     QTVTATGRLSSSNPNLQNIPVRTLEGKRIRQAFVAPPGWCIVSADYSQIELRVLAHLS
                     GDENLLDAFASGADIHGRTAADVFGVFPEMVNPEMRRQAKVINFGVLYGMSAFGLARE
                     LDITQRQAQLYIDGYFRKYAGVRAYLDAVLEGARRDGYVSTLLNRRRYLPEIVGSNAT
                     VRQFAERMAVNTPIQGTAADLIKIAMIRIHRRLREERHSARMIMQVHDELVCEVPAAE
                     REAVIDLVKAEMEGAYRLKVPLTVEIGFGANWDEAHGG"
     gene            43132..43581
                     /locus_tag="PLW16_03300"
     CDS             43132..43581
                     /locus_tag="PLW16_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPB69966.1"
                     /translation="MEPRKEFAAPCGLYCGVCGIRIAHENDDTNFKERLAPIYGLTAS
                     DIRCKGCLSDDPFVLCRVCHIKTCTREKGYEGCHQCADFPCRYIEEFPIAVGKQVILR
                     AIPQWRELGTAAWMAAEEKRYLCPHCGYKLFRGAKRCRKCKEPVALD"
     gene            43592..43945
                     /locus_tag="PLW16_03305"
     CDS             43592..43945
                     /locus_tag="PLW16_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPB69967.1"
                     /translation="MAGIIEEDRRPWGYYRVLADEADHKVKRICVYPGKRLSLQRHHR
                     RREHWYVVAGEAVVTRDREEIPLAAGRAVDIPRGAAHRIRNAGGTNLVFIEVQTGTYF
                     GEDDIERLEDDYGRA"
     gene            43985..44848
                     /locus_tag="PLW16_03310"
     CDS             43985..44848
                     /locus_tag="PLW16_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPB69968.1"
                     /translation="MKQTVPWTGVILVAVVLVLIGGYLHISTDAERLPENRPEVFLAQ
                     PAAKPGARVVVCAGDSLTHGAVSVNYVELLGRTFRDGGYEFVNAGVNSSLAYNLNRRL
                     EDIIRCDPDYVTILIGSNDANASLGDPTARRLIRKMKLPRRPDRAWFRSNLVAVCEEL
                     KARTHARIALLSLPPIGEDVDSVAFRRAAEYSGIIKEVARSQNVDYLPLNEAMTAGIR
                     ARGQKPTLSHTGDTQLPLYAALAKHYLLRQSYDDISAGNGFLYLTDLLHLNTRGATLV
                     AGFIGEFITRK"
     gene            44871..45719
                     /locus_tag="PLW16_03315"
     CDS             44871..45719
                     /locus_tag="PLW16_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB69969.1"
                     /translation="MKNINIPRIDFHVHLFPDRMCDAIWEFFARDYRWDVLHKLYHRE
                     CIQYLREHGIERIVYSNYAHREGVAEGLNEWNARVLDENPDLYCFAAFHPGDEHALDQ
                     AEEILDHPRVLGFKLQLLVQCFFPHDERLFPLYERVMDRGKRILFHVGTGPVGNAYVG
                     LGPFRKLLRRYPDLPANVAHMGAYEYQGFMDLLDDHPGLYLDTSYTFFKDWQGRGGFD
                     LGNAPLEKYKDRILYGSDFPNIILPRESEIETLREYGLSREFYDRVFYDNGLKLIDAT
                     VAPMAE"
     gene            45821..47413
                     /locus_tag="PLW16_03320"
     CDS             45821..47413
                     /locus_tag="PLW16_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPB69970.1"
                     /translation="MNLDRHHRREVDRRRNFGIISHPDAGKTTLTEKLLLFGGAIQQA
                     GAVRARKAARHALSDWMGIEKERGISVTTSVMKFNYRDYEVNLLDTPGHQDFSEDTYR
                     VLTAVDSALMVIDSGKGVEPQTEKLMEVCRMRNTPIITFINKLDRDGLPPLTLLADIE
                     DKLQIECVPMSWPIGMGKSFRGVYDLYRRELHLFTPGGSTQAREGLVITDLEDPRLDE
                     LLGTQAGELREHVELVTEAANPFELTQYLKGNQSPVFFGSAVNNFGVKELLDAFVEMA
                     PAPVARPTLTREVSPYETAFTGFVFKIQANMDPAHRDRIAFLRVCSGTFRRGMKVMHH
                     RIGKEVTLTNATIFMSQDRTNVEEAYPGDVIGLHNHGTIKIGDTFTETEPLKFIGIPS
                     FAPENFRRIHLKNPMKSKQLQKGLAQLSEEGAVQVFQPLGKSDYILGAVGVLQFDVTA
                     ERLRTEYGADTVFEETPYNVARWVACADPKRFKEFERENGSSLALDAEGRPAFLTASE
                     FRLERCMESWPDVAFLKTREYA"
     gene            complement(47461..47832)
                     /locus_tag="PLW16_03325"
     CDS             complement(47461..47832)
                     /locus_tag="PLW16_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69971.1"
                     /translation="MVVAAFGLAAAGGAVARFARTRRGWLTGHRRLGLGGAGATWLGA
                     AFAVAMVAGAGGGHLSSPHTWLGLATLVSALLTPVLGQASFRIRGKAALLRTIHRWSG
                     RTTLVLMAVTLATGLIAAGIV"
     gene            complement(47900..48301)
                     /locus_tag="PLW16_03330"
     CDS             complement(47900..48301)
                     /locus_tag="PLW16_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69972.1"
                     /translation="MHPKNRHRTTLKPLITVVGLLLAFTVAAAFLPAEAGAHGPKTVR
                     LAYDAAAQTLSVTISHPSPNPGFHYIKRVVVRKDGKDLETTEYKSQPGTPEFTYAYKV
                     AAVGGDILEVKVTCNIWGSKTEKITVPKATK"
     gene            complement(48367..49200)
                     /locus_tag="PLW16_03335"
     CDS             complement(48367..49200)
                     /locus_tag="PLW16_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB69973.1"
                     /translation="MLIRCWGARGSIPVSGQEYLKYGGDTTCMEIRTKDDEILIIDAG
                     SGLRRLGNRLLEEKRRRYTMLFTHAHWDHLMGFPFFRPIYIRGTDITLFGCPFAQSSI
                     KELLANIMTAPHFPVNFEDIRGKITYHEACDESFTWSSLTITPIPLSHPNQGVGYKIV
                     EDGKSFVFLTDNELTFQHEGGLTRADYLDFSRDTDLLIHDAEYTERDYHRAWGHSRYG
                     DALDLALQANAKMFGLFHHNQERSDDGVDAMVDDCRKIVSERGGPLQCFALHQDMEIH
                     L"
     gene            49399..49596
                     /locus_tag="PLW16_03340"
     CDS             49399..49596
                     /locus_tag="PLW16_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPB69974.1"
                     /translation="MPTIKIRGMSCQHCVMAVTKALAGIEGVTDVRVDLKTGEASYTE
                     TKPVDRDVIRGRIQKAGYEVV"
     gene            49607..51865
                     /locus_tag="PLW16_03345"
     CDS             49607..51865
                     /locus_tag="PLW16_03345"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPB69975.1"
                     /translation="METTTIAIGGMTCAACVRRVESALRQVEGVTDVVVNLATARATL
                     SHRLQWAGLEALRDVVTDQGYVYLGVPGEDRSDPVQEEREREIRDLARRFVVGAVLSV
                     VIFAGSMTQGLPLLRAVPRPALLWVLFLLTTPVVFWVGSRFITGAIKAARQKTSDMNT
                     LVAVGSLAAYLYSALATFIPELFAGAGVAAHVYYDGAAMIVTLVVLGRLLEARAKGKA
                     SLAIRRLIDLKPKTARVIRGDDEVDVPVETVLIGDVLRVRPGETIPTDGVVLAGSSMV
                     DESMLTGESLPVTKEAESPVFAATLNRAGTFTFTATKVGKETALARIIRLVEEAQGSK
                     APIQRLADRVAAVFVPAVFAIGLVAFAVWTVCVPQAGFSLAMLNFISVLVIACPCALG
                     LATPTAVMVGTGLGAERGILIKGGESLEKAHKLTRVIFDKTGTLTRGAPEVTDVRVAD
                     GEDPGRILQAALSIEAGSEHPLGQAIVARARERGLAPRPVERFTALAGFGARAVIGAE
                     TWLLGNRRLLEAEGISPGGMADAARHLTADGKTCVFVVEGRRPVGLIALADEPRAEAR
                     EAVAALKAMGLKVAMLTGDNRATARAIGDRLGIADILAEVLPGDKAAEVRNMQAKGET
                     VAMVGDGINDAPALSAADIGIAVGAGTDVAIAASDITLMRNDLTAVADAIRLSRQTMR
                     TIRQNLFWAFVYNAVGIPVAAGALYPFFGILLNPEYAAAAMALSSVSVVTNSLRLRRR
                     WRKQSGPVPRVP"
     gene            complement(51862..55098)
                     /locus_tag="PLW16_03350"
     CDS             complement(51862..55098)
                     /locus_tag="PLW16_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB69976.1"
                     /translation="MILHKTFLRAAAITLALLGLSVDFAPAQERPEIFVQLGHSGVQS
                     AAFSADGKSILSGGDKTVKLWDVASGREIRTFAEEHAGYVSSVAFSPDGRRLLSGGTE
                     GRIVLWDPVTGSKVGDLAGHAKGVFRAVFSPDGRVIASGGMDAAIRFWDVATGRQIGV
                     LVGHAAAVRTLAYAPDGRFLVSGSWDRTVKLWDVSTGQEIRTFTGHTAAVNAVAFSPD
                     GHYLLSAGGKDGTVRYWEVSSGRERAVLRGHRGEVTSIAFLPDGRTAVSGSFDRTVKV
                     WDLDRAVELKTLPAGEVSVFSVAISPDGARLLAGSRNMSLWDLATGGELRRLDGKVNW
                     VHAVALSPRGEYVLSGHFDGTIHLWDGITGKHVRAFRDDRGIVSSLDFSPRGPMLECR
                     LDGPLTLREVESGRAMSQLDGRGAAAFSPDGNRIASGAAGHRVQLWDAATGRVIRSLA
                     GHGGGIRSVAFSPDGRFLATGSRDKTVRVWDLESGLERRVFDDFRAPVNSVAFSPDGK
                     RVLAGSSDNTVRLFAVESGAQLQVWRDIAISSVAFSPDGRHVLTAGFDGVPKLLDGAT
                     GRRVMGFQGHKLHVYGARFSADGKRIVTGSGDGSVRLWDVASGREVVQSIGFTNGEWI
                     VITPEGYYNSSARGHELLNIRRGSQVYGIDQFYDVFYRPDIVSAKLKGEDLAGLVTLT
                     VEEAIQNPPPSVKFSPLPHETGDPTVKICYQVRSTGGGIGEVRLFQNGKLIKSDGFYR
                     EAAAQGATAAMKLAQLDGGAVHRDMRSLTLREKQSPGAVMVRPKGDRVDECVELEAIA
                     GENEIGLTAFNASNTVQSMMDTVRFTSTVEPDEPHLYLLAVGIDRYRDAAINLKYAAK
                     DAQDFLSELADRSGTLYRPTHIHPTGLFNEQAVKQNILATIEKLVARVKPGDSFIFFN
                     ASHGLLLQSQYYIVTGGFDGRLDGGAGLISSNEIVEMSKRIKALSQLFIFDTCHAGGV
                     DTIVSGLYDARMSVMAKKMGLHIYASAGSVQTALDGYRGNGLYTHTLLEGLKDGRTVD
                     KDGSGAVTVKGLGRYSKEMTEAISGRLGHPQTPLIMNFGKDSRLFEVR"
     gene            complement(55117..55317)
                     /locus_tag="PLW16_03355"
     CDS             complement(55117..55317)
                     /locus_tag="PLW16_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPB69977.1"
                     /translation="MADTAPPPAPAPEKAARYVCSVCGYEYDPEKGDPDSGISPGTPF
                     EDLPDDWTCPVCGAGKSQFEKA"
     gene            complement(55384..55812)
                     /locus_tag="PLW16_03360"
                     /pseudo
     CDS             complement(55384..55812)
                     /locus_tag="PLW16_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            56008..56436
                     /locus_tag="PLW16_03365"
     CDS             56008..56436
                     /locus_tag="PLW16_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69978.1"
                     /translation="MVTVRSGPGRIAAGLMRIRYVREAVAEKADLSGLSSRPTLRTWT
                     GLGMMAFSYVIGWPAVGLLVWLSYRLREPLVAVVGGTLAYGLSHLVFMAGAALAGVDY
                     AKPVLRWAVRRLVERLNGRDAVPESEETNDRPGKTGVEHP"
     gene            56450..56716
                     /locus_tag="PLW16_03370"
     CDS             56450..56716
                     /locus_tag="PLW16_03370"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPB69979.1"
                     /translation="MEGFIAELKDKIIETLGLTDVRPEDIPVDGQLIGSELGLDSIDV
                     LELVMMIEKDYGVKIENREVGVKVFASLRALAAYIDANRREKDR"
     gene            56713..58869
                     /locus_tag="PLW16_03375"
     CDS             56713..58869
                     /locus_tag="PLW16_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPB69980.1"
                     /translation="MNGVRAFVCGTGIVSAAGEGVEATWGALRRGTSRLTPLALFPAA
                     HTPPLPVGEVRDVPEVDGLPRTHALAWMAVRQVLAATTLTPDALVLGSTTGGMPTTER
                     LLEAGERRPEAYRHHAVGSVAAWLARRLSCRGPVITVSTACSSGTAALKIALELIRCG
                     QARVVLAGGADALCRMTYYGFHALQLIDPAGARPLDRDRRGLSVAEGAAFVLLVAAES
                     PPVGALAELAGAGWSCDAYHPAAPRPDGTGALAAMRAALADAGMTPAALDYINLHGTG
                     TPDNDLAEARAVRALCGDPPPVSSVKGLFGHTLGASGAIEAVVSALGIHRGFIPGNCG
                     CRIPDTALGLLPELAGQSRPVRSVLSNSFGFGGNNASLVLTAPLSAGPARPPRPLARF
                     SVLGTACLTGAGPLEETLARYWRGEPFSGRPDLAAVRTEIPAGQIRRLKRLSRMVLLL
                     ALAARQAARPPAAPGGIFFGTAWGSLTETHDFLKKLFDSGERFTSPTDFVGSVHNAPA
                     SQAAIFLEATGANVTVSGGDHSFEQALYTAGLVLRAGDPPALVIGAEEDHDVLSPCFD
                     AAATADGGGAFLLDPRGGGTGPDLVPVHFAFNPGGDDGLPALIESLGGAARIAERFGA
                     VFVGLPAGDRSGGEAQLERFRRETAFSSPVVDTRRFTGEFAGASAVAAVLAVETVRRG
                     ALPPALAGGEDPRLGGRGILLLGLGEFITAIEVSAP"
     gene            58866..60215
                     /locus_tag="PLW16_03380"
     CDS             58866..60215
                     /locus_tag="PLW16_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HPB69981.1"
                     /translation="MKVLLISANVTTNPYPVYPIGIEYVAGALAPRHEVHLLDMNVPD
                     GPDPIRRAVGEFAPDLVGLSLRNIDNVDTVNPQSYIDGYRDLVERLRACTPAPLVLGG
                     SGFSIFPGELMALLKADFGVVGAGETLERLIEALETGADPSAVPGVAVPGGEIPEPVV
                     WTKTRARRIQPPPALLEFYLKRGGMLNLQTQRGCPYRCLYCTYPHLDGCRPCPDDPET
                     AGETARHLQDLGAGYLFITDSVFNADWEHSLSVARAFRRHGVTVPWGAFFAPTRPPGG
                     YYEALREAGLSHVEFGTESLSDAMLHAYRKPFSVAEAVAAHEAVRRAGLHIAHYFLLG
                     GPGEDGRTLDETLGNAETLERAVFFFFCGVRIFPFTPLHERALAEGQLAPGQNLLTPV
                     FYRSPGIAAPDIMARIHRQAGGRNNWVYGDGGRESERIVSRLHAYGHPGPLWELLLR"
     gene            60212..61057
                     /locus_tag="PLW16_03385"
     CDS             60212..61057
                     /locus_tag="PLW16_03385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB69982.1"
                     /translation="MKGTGPDRRGRGACSPAHVTGGAAFLTAGLLGFTPVPEAAAVPL
                     GVFVLACLAAPFYPRSRFFLPVISRGKRVDRAVALTFDDGPDPRVTPRLLDLLERHGM
                     PATFFVAGNRAEAFPGLVRDILARHHAVGNHSYRHDPFLMLRSRSRLREEIARTQALL
                     SPLGVRPLAFRPPVGITSPRLPGVLESLGMDCVTFSCRGGDFGNRRVGGLARRVLRRV
                     HPGAIVLLHDVAPSASAGVPGWLEEVERILTGLEARGYRVRPLAELIGRPVMERRPPS
                     DRDFS"
     gene            61212..61469
                     /locus_tag="PLW16_03390"
     CDS             61212..61469
                     /locus_tag="PLW16_03390"
                     /inference="COORDINATES: protein motif:HMM:NF003757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB69983.1"
                     /translation="MSREEVFNLIREFFVEEFEIPDEKVIPEADLFEDLGLDSIDALD
                     MVGMLEARLDIEVLEEELKKIRTIGDVVDYISVKVGDRGDA"
     gene            61466..61867
                     /locus_tag="PLW16_03395"
     CDS             61466..61867
                     /locus_tag="PLW16_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FabA/FabZ family ACP-dehydratase"
                     /protein_id="HPB69984.1"
                     /translation="MRRPFRYELSLSPNAGGDRIAASARIPADSAWFDGHFPDNPIVP
                     GVALIGLVEEAVLRSERDRGRTVEMTGIRRVRFRLPVKPDDPITLQAVRDVKRGTPAF
                     SFNVCLEGEIACSGILAVAEAAPTDEHGPLA"
     gene            61864..62787
                     /locus_tag="PLW16_03400"
     CDS             61864..62787
                     /locus_tag="PLW16_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB69985.1"
                     /translation="MTIRVDHLFKSYGANLVLNDVSLDFREGAITGLLGPNGAGKTTL
                     VSILMGIVAKDRGRVLIDGLDLDLDRDRIQARASIVPQSFAFYPTLTARENLEYFGAL
                     YGFKGRRLKARMDAAIDAGSLQSFLGKRAAELSGGMKRRLNLAIGLLNEPAILYLDEP
                     TVGVDAQSRSYLLDRIDRINRERGTTILYASHYMEEIEQISQDIAIIDGGRIILHDRK
                     EDILARRGAVTLTLQHLPGSLKEALDRVAGLRVEVETLVLDRNEDLNRTLAGLFDVLA
                     RHGAEVRALNWGNGHLEALFLELTERTLRDE"
     gene            62784..64013
                     /locus_tag="PLW16_03405"
     CDS             62784..64013
                     /locus_tag="PLW16_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB69986.1"
                     /translation="MNRIYHLLKKEFLVISRDVHAVAVLFVMPMAFILIMSLAMQDLF
                     TTQSPVRIPLLVSNRDGGKPATALLKTLRENASFEVKDIPATWSDAEAAAALKDDDAK
                     FVILIEDHFSAVVRDRKPRTGRKPLVVLVNPVVGTQMQLIVQSVLANALGKVRIEVMT
                     KEMSPLLELARIDPRTFRRPSEAHIEMRFVTRGEQRFRAPNAVQQSVPAWLVFSMFFI
                     VIPISNTFISERSQGTLQRLRSIRLSRGILLLGKMLPYFFINLIQVVLMLLVGMFLVP
                     ALGGSALTLGDSPAGLALIAAAVSFSSIALALLIAVVARTTEQATTLGGLLNIVFAAA
                     GGIMIPKFVMPAVMRKVAELSPMSWGLEGFLDILLRNGGVRDVLPECLALVAFGAVML
                     LLTAAILKKQFEGSVSP"
     gene            64010..64273
                     /locus_tag="PLW16_03410"
     CDS             64010..64273
                     /locus_tag="PLW16_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPB69987.1"
                     /translation="MKGSLDNNLKMDLKRLIIDACDLDVEASDIDDDGLLFGRGSQLG
                     LDSIDALQISIAVQKYGVKITDSKQMRRVMASINTFADFIRPE"
     gene            64278..65444
                     /locus_tag="PLW16_03415"
     CDS             64278..65444
                     /locus_tag="PLW16_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPB69988.1"
                     /translation="MRNRAMIKGRAIACALGEDLDAVVRGAREMRPGIARLPLTLAGM
                     DEVRPYYLLSRRGPESLSAAPETFFYEVLFDTVERALADASLRTADLRDLAIFFGSTA
                     IDIPVYEHAYGQTLRETSRPASMVSSGYGRIADEVAGRFGIRGPTYTFTTACTSSANA
                     LLYAAAMIAAGRIPRALVIGYDLFSHLGFYGFESLRLIATDGYRPFDRRRSGIIMGEG
                     CGAVVLEADDGGDAGAVLCGGANRVDAYNVTTHALEGGAIAQVMWDALEDADVEAGAV
                     TAIKAHGTGSENNDRMEGNGMVRVFGDALPPFTVLKPLIGHTVGASGVVELILFTACL
                     QRGFCPPTLGFEEADEALSLRPVTEPKPLSRGVFLLNFFGFGGNCTALVVAVRG"
     gene            65450..>65807
                     /locus_tag="PLW16_03420"
     CDS             65450..>65807
                     /locus_tag="PLW16_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69989.1"
                     /translation="MFIHAVSQFVALPAANPRTLKEVLKGYTDTPFRRVNKFILLALV
                     GAHRCVRGRTLNGNASVYLTTENGNLGDTNNVLDQLYRQHSLPMPFSFINTMSNTAPF
                     YVAQSLNVLGRNLSISG"
BASE COUNT        11296 a        20953 c        22155 g        11403 t
ORIGIN      
        1 gggaccccgc ttcaacccct tggcggcctc cttcagttca gcgagaagca cggcggcctt
       61 ttcggcagtc caatgcttct tgtccttggg gtccagctcg ctcgtccacc cgatgccttc
      121 ctcgacccgc cgcccgtccc tctggtacct gatggtaaaa tacctgtccc tgccgacccc
      181 atgttttctg gtcgggtgtt cgtaataccg gacgccgggc cacttggtgt tcttccatat
      241 tacgggcatt gtctcacccc ccgatcttgg tttcccgcta atcctctccc catcctctcc
      301 ccactctctc cccactttta ggctgaaaat gcctgaaacg tcatgatagg ttgtgacgaa
      361 gaatatcgga atttaggtta attgtcaagt acttaatgga gggtcgtgat ggtttctgaa
      421 atgctacaaa acggattcct aatccgggtg ccgcgcgttc gaatcgcgcc gggggcacca
      481 attatttcaa ggggttacac gacaatatcg taaccactat ttatcgacac cccccgattg
      541 tccccgttct ctccccgttt aatttctgtc tggaaggtat atttacccct ttcacaggac
      601 cggccgcgtt tttgaagggg cggcatcggc ggaaaggtag ctccggagga acagccgttg
      661 cgggggcgtc aataccgtcc ggtgacgcaa ctcgtggatc cgccggatcg cctgctccgt
      721 ttcgagggat tcctgccggg caagccacgc gcagagaacc gtgccggttc gtccgcgtcc
      781 ttccatgcat tgaacggcga cgccgaccgg gagagaggct tcgatgaagg ccagcgcgcg
      841 gtccatgccc gccgccgttg gcggtccggc atcatcgatg ggctcgtggt ggtgacggaa
      901 gccggccttc cggtgcctgt gcccgtaaag gtcgtcttcc gtcagggtca agatggcccc
      961 gatgcccagt tcgcgcagga gcttgtgggt gcgctccatc ttccccagga cgggctcgcc
     1021 catgcccgcc acccggtacg acccgagaaa accgaagttg atttcctcga gaacggatgc
     1081 atctgtttca acaggttcag gtctcataga tcgcggtatg gtttactccc ctgcggaacc
     1141 ggccattttc cccttacgga tcaagtgctt ccgctcttgc ctttttctgg tacccgatga
     1201 ggagcggaat gcgggaggga ctgtcaatca aggtcctgaa cagaataacg cccctggggc
     1261 tgggggccac aaccaccgtc gtgtttttcg ggacagggta gggtgcgttt ttcttcacaa
     1321 ggacaacgtc ggcataaccg ccttttaccg tgcggtcatt catcacataa aagttgtcgg
     1381 tattgatggc gaagaaagag gccgaccagt aagaaggggg aacgggggcc atgatccgaa
     1441 ccggcctctc gctgacgtca taggccatcc ccgaatagag aagatcgggg cagggcctga
     1501 cgaccttacg cgtgtctgaa tttaccactt cattatggta gaccgtattt gtgggaatgt
     1561 caaaatcgtt ctgaatcctg tagatcacgt aattcataat gatatcaggc aggaaataga
     1621 caaaggcgac atgaaagacg attgtcaagg cgaggacaaa cagtaagcag gaggatagct
     1681 ttttcatcgg cacccctcct taatgatacg gggcagtctg actgaagcgg gattcttcag
     1741 gacttcgctc cccggattat aaatgcggag aaacaggtag agtctctttt cgtcgccagt
     1801 ggggatccag tttcctatcc tggatgtgga cgaaagccgg accgtgtagc gtgagctacg
     1861 gccatcccga aggattgccc ggttgaacga gaaacgattc tcgccgtttt ttatcagata
     1921 ctggtctgaa ccgtacacgg taacgctcca ccacctcgcg tcaatatctc ccccttcaat
     1981 tcgatagacg caggcggcgg taaggggggc gccgttatcg tcggtttcag cgtaataata
     2041 tacagcctcc gatctgttga gagacatcag ggatatgagt gcgacattgg cctttgaata
     2101 catgtcgagg ttttctgagc cgatggaggg attggtgcgc caggcgccat ttgtgaccca
     2161 tccccgctga cccgtatact tgattgccag aaaagcgctg gcggcaccca gaacaagggc
     2221 cgttatcagg catcccgcga tgatcagtat ttttttcatt cccgcgcctt tcctacaggt
     2281 attcatctgt cttcaggaag gttgatccag aaactccagg acggcggcat tgaagcggtc
     2341 ggcctgttcc acattcacaa gatgggcggc gccgggaatg accctgacca cggatgacac
     2401 gatccgctcc ccgatgaatt ccgcctccga cacgggtgtg ccctggtcct gttcgccgac
     2461 gatgatgagc gttcgggcgg agatgttgcg gatggcctca cgaaagtcca tcccgcggat
     2521 cgcccagccg catccgcaat atccgtccac ggatgtgttc aggatcgccg ttcggatttc
     2581 atcgatttcc cgttggaggc gccgccggcc gtcttgcgtg aaccagcgat cgagggtggc
     2641 atcgaccaat gccgccattc cgccccgccg ggtcgcctcg atgcgctcat cccatatctc
     2701 gggaaaaggc aggtaggatg ccgtggcgca gaggatcagg gaggacaggc gatttccatg
     2761 gtacgcgccc aacatctggc cgaccatccc gcccagcgaa atgccgcaga aatggacctt
     2821 ttccagcccg agaaaatcga tcagacccac ggcgtccctc gccaacatct ccatggaata
     2881 aggcccgggc gatgaagcgg actgcccgtg gccgcgaccg tcatagcgaa gcacccggta
     2941 gccggcatcc gtcagcgggg gcacctgaaa cttccacatg tccaggtttg ccgccagcga
     3001 attggacaac atcacggttt ccccgcccgc gggtccgtcg agccggtagt tgagcatcgt
     3061 tccgtttatt tgcgcccgcg tcatagtcac cctccgaatc ggtattgatg cgcccggtcc
     3121 atggacttca cccaggcggg ttcacggcgg cgcgaccatc cggtcgaaat cgtcccgcag
     3181 cgtgccggcc aaccggttcc ggaccccgtc gggatcgtgg acgatgtcgt ggatgatctt
     3241 cttcgtgtcg ttgaggttgc tcgtgaagcg gcggtcgatg cggctcagga gcagcgcgga
     3301 gaagaggcgc atcgccagcc gcgagatccc gttgctgccc cgcatgaaga tcttcgcctc
     3361 tccggcgagc ccgcccccgt ccggtttgca ctcaaaagcg accacgccct cggcgctgaa
     3421 aaacgagggc accgcccagt gccggaacgt gcccgatccg tagaacgccc gggaggcgtc
     3481 cgaggggggg agcggccgca ggtcgccggt gatgccggaa ggatccacca cgcggaggtc
     3541 cgctccggat cgggtcaccc ggtaggcggg ctgaaaccgg tactgggtcc agagctgggt
     3601 catcaggtag ggatgatcca ggattcggtt ccaggtctcc ggcgtggtct cgacgcggaa
     3661 ggccttgtga taggagaggg tcgggtcgcg gacgacgcgt gcggcggggt cgtcgtccgc
     3721 ccgcgcgggt gcggcggcga gaatgaaaag tgcggccagc gccgccatca gataccgttg
     3781 gatcatcggg gtcccccggt cgttcaatcc ggaatccgcg gcagggtcac ggtcttcccc
     3841 tccgcgttgc gcagacggca ggatcggccg gcgcgggacc ggcgctcccg cgcctgaatc
     3901 gggatcttgc cgatattcgt cgagacgacg tagtggggct gggccaggcc ggtgatctgc
     3961 ccctggaggg cgtcccggat cagctccgcc cccttttcga gcggcgtccg gaaatgatct
     4021 atcccggggg cgaaatcgca gtagaacagg taatagggcc tgacccggat ggtcaggagg
     4081 cggcggtgga gttcacggaa cgtctccgcg tcgtcgttga tcccgcgcag caggaccgcc
     4141 tgattcccca cgcttacccc gcaggaaaga agatcgtaaa cggcgcgggc catcggttcg
     4201 gtgatctccc gggggtggtt gcactggagg ctgatccaga tcggaacccg gtggtagccg
     4261 cggaggattt tcatcagccc cggcgtgatg cgctgcggca ggacgacggg ggtgcgcgtg
     4321 ccgatccgga tcagggtgat gtgggggatc tcccgcagtt tgcggaccag gtggtcgatt
     4381 tcggcgtcca cgaggaggaa cgggtcgcct ccggagatga gcacatcccg gatttccgtg
     4441 tgctcgcgga gccagcgcag gccctcttcg cgctccaggc gcgggcgtcg ccccgggcac
     4501 aggatcaggt cccggcggaa gcagtggcgg cagtagctcg cgcagcgatc tgagacgagg
     4561 aaaacgacgc ggtccggata cgccttgagg atacaggcgg gcgtggcctc ggccccctgt
     4621 tccacccagg cgaggaagtc cggccggccg tagcggttct cgcgctccgc cagggaaggg
     4681 ataacctggc ggcggatcgg gcaggcgggg tcgcgggggt ccatgagggc ggcaaaatgg
     4741 ggcgtcgtcc cccagaccgt ctgcagggtc gcgatcgcct cccgttcgcc gggcgtcgtt
     4801 tcgatgaacc gttcgagtcg ccgcagggtg ttgaccgggt gcttgagctg ttccgtccag
     4861 gattgcccag ccgcgctctc caaggctcgc gcccgcggcg ccggtgagcc atgtccgtgc
     4921 cggttcaact tttccggctg gttttggccg tgaagctgga aatctgctgc cggaccttcc
     4981 tgctcttgca tttcggacag acgatctttc cccggtcccg ctcggagacg gagagggtca
     5041 acgaaaactt gcgattgcag gcctcgcagc gatagtcgta cgtgggcatg gtcaaacctc
     5101 ctgaacccct tattttatcg gaattcctcg atacgttcct tcatgcggaa gacctggccc
     5161 ggattctaac caaaaggggc gggagcggtc aacccctttt tgccgcgacg gtcggtccgc
     5221 gcgtttcgcg ggcgcggttc cgggagggct gatccgatct cggtggaggc gcaagccggc
     5281 gccgagcaga acggaggtga cttccgggtg gagaatcgtg tataacggac gtggacgaaa
     5341 cccggacggg aggaggcgcc gatggcgaac atactggtcg tttatcattc gcagacgggt
     5401 catacggagg ccatggcccg ggcggtggcc gagggggccg ctgaaataga gcatgcccgc
     5461 gtgaccctga agcgggccgg ggaggccggc ctccaggacc tgctcgacgc tgacggaatc
     5521 gccgtcggca cgccggagaa tttcggctac atgtcgggca tggtcaagga cttcttcgac
     5581 cgcaccttct atgccgcgca ggacaaggtt ttccgaaaac cctatgtcgt cttcatcagc
     5641 gcggggaacg acggccgcgg cgcgctccag gcgatcgagc ggatcgccct gggctacaag
     5701 tttcggaagg tctacgacgc ggtcatcgcc cggggggagc tgaccgagga cgtcctggcg
     5761 cagtgccgcg aactcggggg cgtcctgtcc ggcgggtgcg aggccgggat ttactgagtc
     5821 ccggggaagg cgccgcgccg cccgaacggc cggacgttca ataggtccag ccccagacgc
     5881 ccagtgcaag cacgttcatg acgaccggga cggcgtagat caaccccacg atcgtcagtt
     5941 tggcccgcag cgtcttccgg ccgatgacca ggatggcgta gcagaaaaac tggaaatagc
     6001 cgacgaggaa gatcagccag atgcccttga acgacagaga ccaccaaggg tattcccaga
     6061 cgaggtgtcc gccgacgttc agcacgcatt cgatgaagac gcagaaaacg ctgtagccga
     6121 tcgcccagaa ccacgtttcg ggcaggccca ggattcgctc cttgcggctc tccgacaggg
     6181 tgtgggtgta gatgatgccc agcaacagga acatgaacat gatctcgatg ttccagccga
     6241 ccatcgtcct caaggcggtg tccccgggcg tcgtccagaa ggcggagcgc tgcgtcagat
     6301 gcatgaccca gccgttccag gtctcgttga agaagtcggc gccgaagacc gtcaggccgg
     6361 cgaggacggc gttccagtcc cgggtccttt tcgcctcctt gatttcacgg gtgtagacgt
     6421 agaagacgat cgccatcagg gggatgacgt accatttgag catggagagg tcacgcaggc
     6481 cgcttaacgc ttgctgggtc gcttccgtca tctcgaacct ccttgatccg gttgaaggtt
     6541 agaggtgcgg cgcatcgggg gctcccgtcc gcccgatgcg ccgcgccggt gaaccgtcgg
     6601 gcatcgatcc ccgctatttc catcttttca tgcatgcgcc gcacccgctg ggcgcagtcc
     6661 atacagttag ttgttcgcgg acccgttgtc cgtaaggtgc aaaggcctgt cccttgaacc
     6721 atagggcaaa gcccggcccg tgtcaattgg aagatcgggc gtgcgttttg tctggagacg
     6781 gagattcggg acgcgatccc tataccgggg cctccggctt ccagcccagg tagaggtagc
     6841 agataccgaa catcagaggg tggcgggcga tccggacgag ccccgcctcc gccatgaggc
     6901 cggtgaaggc cgcgggcgcg ggaaagtgga ggatggagtc gcccaggtaa cggtacgccg
     6961 ccggattgcg ggagaccaga cccgccgccc ggggcagcag gaggttgaga tagacgtggt
     7021 agaagggctt gagaagccgg tgctggggga aatgcatttc gaggatgaga acctggccgc
     7081 cggggcgcac gacccgcgtc atctcccgga gagcccgggg gcggtccggg atgttccgga
     7141 tcccgaaggc gcaccccgcc acatcgaagg tgtggtcggg gaagggcagg ttcagggcgt
     7201 cgccctggat caggcggatc cgccctgcga atcgcagctt ccggaatttg gcgacgccga
     7261 ccgccatcat ctcgcgggcg aagtcgagac ccgtgacctg gacgtccgga tgctgccggg
     7321 ccgcctccag ggccagatcg gccgtgcccg tcgcgacgtc gagaaagcgt ctcgttgagc
     7381 cgaagcgcat cttccccacg gcgaaccggc gccaggcgac gtcccggcgc aggctgaaga
     7441 tgcgattcag gatgtcgtag tgcgacggga tcgtggcgaa gacctcgcgc accatgccga
     7501 cccgttcggc ggcgttcatg tcctcgacgg ggggatagtg ctttttgatc cgtggggtca
     7561 tgtcccggat ctccgcgcca taccgcgctg gcggaggtac tcgaacaccc ccggcaggat
     7621 cgacaggacg atgatcgcga cgacgatcag ggtgaagttc tgcttgacga acgggagatt
     7681 gccgaaaaag tagccgccga agagaaacag cgagatccag gcgatgccgc cgatcacgtt
     7741 gaaggccagg aagcggggat aggtcatgct gccgatgccc gcgacgaacg gggcgaaggt
     7801 ccgaatgatg gggatgaagc gcgcgatgat gatcgtcttg cccccgtgcc gctcgtagaa
     7861 ttcatgggtc cggacgaggt gctccttttt gaagaagcgg atcttgtccc gatcgaagat
     7921 gcggggaccg atgtagttcc cgatggcgta gttgaccgag tcccccagaa tagaggcgag
     7981 gcacaggagc gggaacatcc acgccatgtc gagggcgccg atggcgacta gggcgcccag
     8041 tccgaacagc agggagtcac cggggagaat gggcgtgatc accaggccgg tttcacagaa
     8101 gatgacgaga aacacgatca cgtaagtcca gaccccgaac atctcgacga cggtcttcag
     8161 gtgctggtcg aggtggatcg cgatgtcgat cagggtgagc aggaggtcca tgtcgatccc
     8221 gattgtatcg tcccggcggg agagatttca cggttgcggc gccgcccgcg gccatgccct
     8281 tcgaatcagc gtccttaaat gatttccgcc gtcaagtcaa gcggcacggc atcgcgggcg
     8341 gatgccgcac ggccgttccc gggaaaccgg cgggcgaaat cccttgatct ttcggaacgg
     8401 acgtcgtaag cccatcctga cgccctttcc ggcgtgcacg ccaaatccac gacacaggga
     8461 ggtttcagac gccatgtccg aggaaaacta cggtccgctg accctgatcc gcggcgagaa
     8521 ccgcgggcgc tatccctact gccactccgt ctttatccct ggagccggca tcctcatcga
     8581 tccgtcgtcc gaccgcgaac agctgatccg cctgcgcgac gagggcgcgg tcaaaacggt
     8641 ctggttgtct cactggcacg aggaccatct gatgcacctg gatctgttcg acgacctccc
     8701 cctgtggatg gcggaggagg acgccccgcc cctggcggat gtcgaggtgt ttctggactg
     8761 gtaccagatc gaccggccgg acgagcgggc ctattggcgg aagatccttc tgaagcagtt
     8821 tcactttcat ccccgtcgtc cggcgcggtt catgcgggat ggcgaggtca tcgatctggg
     8881 cggggtgacg gtcgaggtcc tccggacgcc gggccacagc cccgggcatc tggccttctt
     8941 cttccgcgag cccaaaatcc tcttcctggg cgactacgat ctgacgccga tcggtccgtg
     9001 gtacggcgat cttcactcca gcatcgagga caccctggcc tcgatccgcc gcctcagggc
     9061 gattccggcc gagcggtgcc tgtcctgcca cgagaccggc gtcttcgacg ggccggtcga
     9121 ggcgctgtgg gacggttacg aggggacggt cttcgaacgg gagaggcgat atctcgagct
     9181 gctggaagag ccgaagaccc tcgcggagat catgggcgcc tgggtcttct acggccggcc
     9241 gaaagagccg agggattttt atgaattcgg cgagcgcgtc catgcgggca agcaccttga
     9301 ctggttgaag gccaaggggc tcctggtcga gcgggacgga aaattcatga gcccatcctc
     9361 agagctttga cccgtacgcc agggcggcgc ggtccagggt caggcgaagc ctctcgtgga
     9421 ggagctcgct gcggccgatg aggaatgggt gatagacgag acgggccgcc tccaccgtga
     9481 cgcgggtttc cttcaggaga gggagggcct ggcgtctgtt gggggtcagg ttcgccaggg
     9541 tgacccgaat gctctccccc gcctcggaca ggggcagcgt gacgggatgc cgggaggcct
     9601 cggggaattg tgcggcgccc tccccgtcgg gctgggcggc ggtcagctgg cgcgcccagc
     9661 ggagaaattg cgcggggttg atcttgaagg cgggcgacca gaagtgcacg ggcgccgcgg
     9721 cgaaggcggg tgtgaccacc cgggggaggt tggcgaaccg gatcaggtcc gcgaaggtcc
     9781 gcatctcaag gccgtccagc cgcgcctgca tccgccagaa gggcaggtag aagacctggc
     9841 cggcggcggt cggcaggacg cggaaggcca cctcctccca gccgtcgttc cggccggccc
     9901 aggccgactg gcagttccgg cacgtcagga ccaggctgtc ccgttccccc cccagatccc
     9961 agccgcagcg ggggcactgg gcgggcagga accggacggc ggcggtcgga tgcgccggcg
    10021 cgtcgagcag cgcggtcagc gcgccggtcc ggtcgtcggc cagcggcagc ggacgattca
    10081 ggatcgcgtc gcatagggcg ttcctgtcga aatacacggg ggtgtggatg aggctcacca
    10141 tctcgccgat gaactcctgg agcccgtcgc cgcccgcggc ctgggcggcg gcggcacgga
    10201 ccacctcccg gcagggcacc ccgggttcca ggaaccggcc cgcttggccg gcggcgcgga
    10261 acttcagctt catcgcctgg ggtcggacgc cgaggctccg cgggaggccg gcggcgtcca
    10321 gggcaagggt gctggtgtcg acgaagcggc tgacgattcc ggacggacgg caatcgtacg
    10381 aggccccctt caggcgccag taggggagga agaggagttc ccggcccgtt gcatcggcgg
    10441 gcggcggcag gaggtaacga aacccctccg gggccgtcat gagcaggcgc gtccggcaga
    10501 atttgcagtt gagcagccgg tccgtctcgt ccaggaggac cggcgccccg cactgggggc
    10561 attgatgttc gatctgccag gcgttcatgg gatctcaatt cttgaggccg ggggcgtgtg
    10621 gcgccgctgc ggcgccccgc ggggccacta tgccgcctga tgcggggatt tgcaagcggg
    10681 aagtgatccg cggcgggtcg cggtcgggat ggggctataa atataagaca tagggattgc
    10741 cagaatatgc ttgcatccgt tccccaccct tgttatatcc actccgtaaa gcaccgggaa
    10801 tctgaggggt ctccggggat tcccggcttg gcgggcggac gggactctcc cggcatctcc
    10861 cgccgggtgg gcgccgcgtc gtcggggcgt cgttgacggt ttttcccccg atcattcatg
    10921 gaaaggagat tgcgtatgct gaaggagttc aaggaatttg ccatgcgggg aaacgttctc
    10981 gacatggcga tcggcatcat catcggtgcg gccttcggaa agatcatcac gtccttcgtc
    11041 aacgacctct tgatgccgcc tgtcggtctt atgctcggaa acgtcgattt ctccaatctc
    11101 ttcgtaaatc tgtccgggca atcctatgaa accctggcgg ccgccaagga ggccggggcc
    11161 gccacgatca attacggcct gtttttcaac agcgtcatcg atttcgtcat cgtggccttc
    11221 gccatcttcc tgctgatccg ccaggtcaac cgcttcaagc gcgaaccgga gaaggcggcg
    11281 accaccaaag agtgtcccta ctgctgttcg gagatccccg tcaaggcggt gcgctgcgcc
    11341 cactgcaccg cggagctgaa atagacgatc cggcaagcgc ggcggcgccc gcgtaaggga
    11401 tgccggttat ctgtgaccgc ggtcacggac ggtttgtgat ggttttcaca taacggcaaa
    11461 tgatccttga caccgtgcgt gagggatcct atgaaacacg atattctata agacatacca
    11521 tcctgtcatg cagaacggtg ggggatctgg agcgggttca tcccccgtgc aaaaatttta
    11581 atgaaagaat tcaggaagga ggagtcatag atgaaaaaga tcatgtttat cttgttgtgc
    11641 gccgtagccg ccctgttctt ggcggcgtcg gccggcgccg aaagggggaa gagcttctcg
    11701 gtcacgccct ttgtcggcgg ctataccttc gaggggaatg aagaccttga agacagtctc
    11761 atcggcggcc tgcggttcgg ttacaacctg acgaacaatc tggccctgga aggcgtgctc
    11821 aattacggcg attccgagta caacctgacg gacgaggatg tcgatcttta cggctaccgc
    11881 ctggaggccc tgtaccactt catgccggac agccgattcg tgccgttcgt ggccgtgggc
    11941 gcgggcggac gatccctgcg gttcgacgac acggaccggg gggaccgcaa ttacctcgcc
    12001 gcggcttacg gcgtcggatt gaaattcttc ttcaccgaca gcctggcgct gcgcgcggac
    12061 gtgaggcatg tgatcccgtt caatgacgtt tacaacaacc tggagtacac gctcggcctg
    12121 acctatctct tcgggggtgg ggcggcgccg gcggcccgcg ccgaggcggt ggtttcggac
    12181 gcggacgggg acggcgtgat cgacagcgcg gacgaatgtc ccaacacgcc ttcgcgcgcg
    12241 aaggtggacg cggtcggctg cccgctggac agcgataagg acggtgtggc cgattatctg
    12301 gacaagtgcc cgggaacacc ggccggcgtg aaggtggaca aggacggctg cccgctggat
    12361 tccgacaagg acggcgtgac ggatgaccgt gatctgtgcc ccaacaccct ttcgggcgtc
    12421 gtggttgaca aggacggctg cccgctggat gccgacaagg acggcgtggc cgatgccaac
    12481 gatcagtgcc cgaatacgcc ggttggagcg acggtggatc ggaagggctg cccgctggat
    12541 tccgacaagg acggtgtggc cgattatctg gataaatgcc cgaatacgcc gaagggcgtg
    12601 aaggtggaca aggacggctg cccgctcgac agcgacgggg acggcgtggc ggattacctc
    12661 gacaagtgcc ccaataccga gaagggcgtg aaggtcgaga aggatggatg ccccgtgctc
    12721 gtggagcaga agcaggaggc caaggccgag gcggtcgtca agaagatggc caaggagaag
    12781 gaggccgttt ccctgctggt ggagttcgac acggcgaagg ccgtcgtcaa gaagaagtac
    12841 tacaacgagg tcaagagcgt ggccgatttc atgaaagagt atccccattc gaaggccacc
    12901 atcgagggcc atacggatag cgtggggaat gcgtcgttca acaagaagct gtctcaggcc
    12961 cgcgccgaca gcgtccggaa atgcctgatc gagaagttcg ggatcgacgc ttcccgcctg
    13021 accgccgtcg gttatggaga agagatgccg attgagagca atgccacgag ggacgggcgg
    13081 cagaagaacc gccgggtcga ggcgatcatc gagtcgacgg ataaataacc agcctgtcaa
    13141 ccgataacag gcgttgaaac aggaaggccc cgcagccgat atcggctgcg gggcctttgt
    13201 ctttcttggg gtcgggacgc cggcgggcgc tcaggcgggt ctcgccttcg gcgggcggcg
    13261 gttgaaccag ggcctggccg cttcgagctg ggcggccagt cggaagaggg cggcctcgtc
    13321 cccgaagcgc ccgatgaact ggacgccgca gggcaggtcg tcggccgtcc agtgcagcgg
    13381 cacggacatg gccggcaggc cggtgaaatt cgcgagctgg gtgaaggggg ttttcgacag
    13441 gctttcgatc gccagcttgt ccgtgatgcc ggacaattgc agtaattttc ccagacccag
    13501 ggcgttgatg acgttcatgg cggtgcgttc ggccggcttg ggggccagtt ccccgatccg
    13561 gacgggggga aaggcgacgg tcggcgtcag gtacaggtcg taggtttcgt ggaagcggcc
    13621 catctgacgc gcggcgatgt cccattcgcg caaggatcgg acgaaatggc ccgcggagaa
    13681 ggtacggccc atcaatccca gggtccaggt cagggactcc acgtcgcggc gggtggcttt
    13741 tcggtccagg atggacgcaa gctcctggat gtcggcggcc atctcgccaa agtacatggt
    13801 tagatagctt ttcgccaggg cctcgccgtt caggtcgggc tgggccgcct cgacggcgtg
    13861 tcccaggtct tcgagcaggc gggccgcctc ctcgaccgcc cggacacatt cgggatggac
    13921 ctccgtgccg atgggggaac gggtgttgaa agcgattttc agtttccccg gttctctgct
    13981 cacctcctcg aggtaaggcc gtttgggcgg cgggatgacg taaggggcgc ctgcatcggc
    14041 gccgcaggtg gcatcgagca gggcggcgct gtcccggacg gagcgcgtga tggcatgctc
    14101 gacgacggcg ccctgccaaa gggtgccgta gagagggccg gtcgggttcc gcccccgggt
    14161 aggtttgagc ccgaacaggc cgcagtgcga ggcggggatg cggagcgatc cgcccccgtc
    14221 gccgcccgag gccagggggg tcatgcccgc ggcgaccgcg gaggccgaac ccccgctgga
    14281 gccgccgggt gtgcgcccgg tgtcccaggg gttccgggtg gggccgtgca gttccggctc
    14341 cgtgacggcg agcaggccga actccggaca gttggttttg ccgaggatga cgagccccgc
    14401 ctgcttgaag cgccggacga gttcgctgtc ccggccaggg atgaaatccc gtcgggcgcg
    14461 gctccccgcg gtgagcggca caccggcgta ggcggccacc aggtccttca acaggtaggg
    14521 gacgccgcga aacggtccgt cgggcagccc cgtctcgacg gcccggcgcc ccaggtcgaa
    14581 catcggctgg atgacggcgt tgagcgcggg atttaaggtc tcgatccgca ggatcgccgc
    14641 ttcgcagacc tcccgcgcgc tcacctcgcc cttccggatc aattccgcga gccccagtcc
    14701 gtcgtaccga tcgtagtcgc tgaacgtttc catcgcttgc cctccttcag ttcgatgcgc
    14761 tgtcttgccg gaacgaaatg gccgccttct cgtcaactgg aacccgccgt gcaaaggtgg
    14821 gatagtcggg gccccggcag ggggttcgca ggggataagc cgggaccgcc cgtacgtcgg
    14881 gtggaggctc gggagttttc cggacatcgg gacgggtccc gtgccgccgg gaatcgggtg
    14941 gacggttcag accggtttcc cgcaccggga gcagaacttg gccccggccg gggtttccgc
    15001 cccgcactgg tggcatttgg gggtttccga ggacgcggcc tgtccgcacc gggggcagaa
    15061 gcgggcgtcg gcgggaagat cggcgccgca gccggtgcac gtgtgggtag cgggaacggg
    15121 ccggccgcag gaggggcaga agcgcgcccc ggcggggacg gactgaccgc agcgcgggca
    15181 gggcgccgtt cccggcgcgg cggacggcgt gccccccgcg gttttgaagg cgtcggcgaa
    15241 catggcgggc atcatgaacc ccatccccat gccgagaccg gcgcccgcct caccctgtgt
    15301 ctgcgaggcg ttctccatgg ccatggccgc cttcatcttg agaagcttgt tcaggtcgtc
    15361 gaagatgccg agcctgctct tgtcgtcgat ggccttctgg acctccggcg gcggggtgat
    15421 ggaattgata tagaggtccg caagccccag tccgaagcgc cggaagtcct cctggaggag
    15481 cccggagagg tcgccggcga gctcgttgta ccgggcgggc agatcgaaga tcgtgtcgag
    15541 gcgctccccc atgaagtcgt tgaagcgggc cacgatcacg cggttcaggt actcctcgat
    15601 gtcggactgt gtgtagaccc cctgcgtgcc gacgaggctg ttgatcagca ggacggggtc
    15661 caggacccgc aggttgaaga cgccgaaggc ccgcagacgg atcaggccca actcccggtc
    15721 ccggaaggcg accgggtcgc gcgttcccca ggtcaggttg gggaagacct tcatattgac
    15781 gaggtagacc tcggcccgga gcgggctcgt catggcccag gggatgctga gcaccttcgt
    15841 aaggatcggg atgttcgcgg tcttcagggt gtggcggccg gggccgatgg cgtcgtaggc
    15901 cttgccctga tagaagaaga cggccgcctg gctgtcgcgg accgtgagct gcgcgcccca
    15961 cttgatctct ccggagccct gctccgggag ccggtgaacc agctctttcc ccgattcgtc
    16021 aaaccactcc aggacttcca gaaagacgac attgtccgtt cccatgaccc catcctgtca
    16081 acggttgtta cggctcggta agttgcgcga ccgggattcg acgacggccc gaatataaga
    16141 gaggggatcg gcgatgtcaa gcgcaaacga cggatgtgcc cgggacgggg gcgccggccg
    16201 ccccggggag ggtatagcga cgggaagctt cgttcggctt tggtctgatt cgatccaggt
    16261 ttgtatttga tctttccccg ggtttgttgt acagatgacg ccatgaagtc caagggaagc
    16321 gaatatccaa ggatatacaa agggatagcg ctcattgccg acccgatcca ccagtacgcg
    16381 gtctttacgg tgcccgcgga ggacggcggg ggcgagaaga ccgaaaagga cctgatcgat
    16441 tccccctggg tccagcgcct ccgccgcatc tatcagctcc agagcgctcg atgggtgtat
    16501 ccgtcggccg aacacacccg gttccagcac tccctgggga cgatgcacct ggccggggaa
    16561 ttcggccgct ttctctaccc gagcctgcgg gaggtctgcc ccaacctgcc gtcggcgccc
    16621 tacgtggaag aactgcttcg cgtcgccggg cttttgcacg atgtcggcca cggtccctac
    16681 gggcacttct tcgacgacca ttttttgaac gccttcagcc taacgcacga ggacctggga
    16741 caggcgatca tcacccgcaa gctcgcccgc ctgatccgcg gactgcgccg gagtcccacc
    16801 ggaactctgg cggcgcggga ggtcctggac ccggcccaag tggccttcct catccgcatg
    16861 ccggatggcc gggacggccg ggcccccgag tggctcacgc tcctgaggca gctgttctcc
    16921 ggcacctaca cggtggacaa cctggactac gttcagcggg atgcctatat gacgggatac
    16981 tccctggaca tggtggatat cgcgcggctc aaattctaca ccttcttcag ccgggagggg
    17041 ctgaccctcc accaggccgg tgtctccgcc ctcagccgct ttttgaatgc acgccttaat
    17101 ctttacgcca acgtctattt ccaccggacc acccgggccc tggacctgca cctccaggag
    17161 atcttccggg agacgatgga tcttctctct ccgctcaatc cgctggtgca cctggaagcc
    17221 tacctggggc tcgacgaatg gacgctgttc caggaggtcg gccggtggcc ggaggcaaag
    17281 gacgcgcggc ggcggtggct gggccgggag tggcggaaac tccaccggcg gcgggtgaag
    17341 tggaagatgg ccttttcgac ggagatctcc gtggaccaga tccagcgcgg cacccggttt
    17401 atccccggga cggactacga gcagcggatt cgggagttcc tgcccgcctc ggtcaagaag
    17461 ctcgccttcc gggtggatct ggcgacccag gacccccggc cgctcaaccc catggcggaa
    17521 acggggaaac ggatcaacat cttcaacccg tcgaccggcc ggacctcccc ggagcccctg
    17581 caggagctct accggttcat tcccgcccgg gtcgtccact tccgggtctt cgccctggat
    17641 cacgaccatg acgacctcct gacggcggcg gcggaagcgg ccctcaacgc gatcgaaggg
    17701 gcggcgacga cgaatctctg atgatactcc ccgaggaaac caccgacatc accctgtttg
    17761 tccggacctc tggcgaggaa atcgcccggt gggaccgccg ccggatcgtc gaggccctca
    17821 tccgcgagac gggaagcgag gccggcgttg cggaggagat cagccgggag gtcgagaggc
    17881 agatcgtcgc ctcgggcatc gccgtgctca ccacgtcgct catccgcgaa ctcgtcgacg
    17941 cgaaactcgt ggagcggggg ctggagcagg cccggcggat gcacgcccgc ctcggttttc
    18001 ccctgtacga cgtggagcag ctcatcctgc acccgaaccg ggagaacgcc aatgtcccgc
    18061 acgggccgga ggggacgaac ctgatcctcg ccgaggggat caagcgggaa tacgccctcc
    18121 accacgtctt ctcccgcggg gtggccgacg cccatatcgc cggcgatttt cacctgcacg
    18181 gtctgggcgc cgtcgaccgg ccgtacagcg cctgccagac gctcgaatac ctgaaggcct
    18241 tcgggcttca gcttccccac gccctgaacg cggcccggcc ggcccggcac cccgaggtcc
    18301 ttctcgccca tatggtccgg ttttccgccg ccctgcaggg gcacttcgcc ggcgtgatcg
    18361 gctggctcgg ggtgaaccat tccttcgccc cctatctcac cgggatgacg gatggggagg
    18421 tcgagcactt cgcccagatg ctcatctacg aattctccca gttgacctcg gcccgcgggg
    18481 ggcagtcgat ctatacggac atgcacctgt cctgggaggc gcccgcgttc ctggccggtc
    18541 ttcccgccgt gggaccgggc ggcaaggcgg ccggagggac ctgcgacgcc tttgccccgg
    18601 aggcgcagcg ttttgcccgc ggcctgatgg cggtcttccg ggccggcgac gccacgggga
    18661 agccctttac attcccccgt cccgtcatcc acctctcgcc ccggttcttc gcgacgccgg
    18721 gacacgaggc gtttctggaa ctggcctgcg acgtggcggc ggacaagggg aacccgtgct
    18781 tcgtcttcga ccgcggtgac accggccacg cgggcctgcc gttctgcccg gaggccgccg
    18841 gggaaccgtg gcgcatgcgc gccgccgccc tgctgaacgt caccctgaac ctgccccgcc
    18901 tcgcctaccg ggcgggcggg gatgagcggc gcctgttcgg actgatctca gagctgctgg
    18961 ccctggcggt ccgtgcgcag gtcgagaaaa aggcctttct caagcggatt ctcggcctcg
    19021 cggcccgcgg ccccctcgcg ctgctcgcga tggaccggga cggcgcgccc tatctcgacc
    19081 tggatcgggc gggcggcctc atcgggatgg tcggcctgaa cgagctcgtc cagatccaca
    19141 cgggcgggca actgcacgcc tcgcccgcgg cccttgcgtt tggcctcaag gtcgtctcgc
    19201 gtctgaagga cgaggtggag gccctcgggc gcgcccgcgg gattcccctg gcgctggagc
    19261 agacgcccgc ggagacgacg gcccaccggt ttgcccgtct cgacctccgc ttccactccc
    19321 cggcctccgg tcgcatcgtc aagggcagca tcgcccgggg ggacgtctat tacacgaact
    19381 ccacgcacct ttacgtcggg gcggcggtca cgcctccgga acggatcgag atggagggtc
    19441 gcttccatcc gctgattccc tcggacgcca tcacccacgt cttcctgggg gccgtccgtc
    19501 cgtccggggc ggcactcgcg cgccttgtcg agacggcctt ccggcagacc gcctgcgccc
    19561 agatcgcctt cacgccggag ttctccagct gcgccgcctg cgggcgcacc cggcggggtc
    19621 tgttcgaccg ctgcccggac tgcggcgccg acgccgtgga agggatcacg cgcatcaccc
    19681 agtacgtgac gggcatttcc agctggaaca aaggcaagct cgcggaattg aaggatcggc
    19741 atcgccatcc gggcttcttt cccgattcgg aataatcccc gcccccggtt cggacctctc
    19801 cgcgaaaaat ccgcgtttcc cggcgaattc ccgctcccgc ccttgacagg gggggaaagg
    19861 ataactaaat taccgccaaa attgaaggaa tgagaatcgg aatccgcatg gaagatcaca
    19921 tcatccggtc ttttaaggag ggcagcgagc tcaaggaggt ctttctcaac gaaaacctcg
    19981 gacatgtcgt gcgggcgatc gaagccatca ccaccgccct gcaggccgga aacaaggtcc
    20041 tcctgttcgg caacggcggg agcgccgccg acgcccaaca ccttgccgcc gaattcgtca
    20101 accgcttcat gatcgagcgg cccccgctgc cggcgattgc gctcagcacg gacacgtcga
    20161 tcctgaccag tatcgggaac gattacgatt tttccgaaat cttcagcaag cagatccgcg
    20221 cgctcggcca ggagggggat gtcgcctggg gaatcagcac cagcggcaaa tccgccaatg
    20281 tcgtccgggg cctggagacg gcgaagaaga aggggctcgt cacgatcggg ctcacgggcg
    20341 gcgacggcgg ggacatcgcc cggatcgtag attatgccct gatcgtctcc tcgcgcaaca
    20401 ccccgcgggt tcaggaagtg cacatcgccg tggggcacgt gatttgcgag atgatcgact
    20461 ataaactgtt tcagcggccc gacgcgccgt aagataacga aaggaacgag cggcaaagat
    20521 catggtaaaa aaaggtaaaa aggactctgt ggtgacagcc gcgtttgaga cctcgtccat
    20581 gccggaggcc ggggacggtc gtccggagac cgatcggacg gtcggtgaag acttggacgc
    20641 gttgagggcg aagcttcatg agaaggaaca ggaggcggcg gagaattacg accggtacgt
    20701 ccgggccgtg gccgatctgg aaaactacaa aaaacgcgcc ctgagggaca aggcggatgc
    20761 cgtcaaatac ggcaacgaaa acgtgatccg cgacctgctg ccgttgacgg acagtctgga
    20821 ccgggccctc aagcaggcgg aggcctcctg cgatttcgag gccttccgga aaggcttgga
    20881 aatgctgcgc gtgcaactgc tgggctctct ggagaagcac ggggtgcagg cgatcgacag
    20941 cctggaaaaa ccgttcgacc ccaatgttca cgaggccatg atgcaggtgg acagcgacgc
    21001 ccatgaggac aaccaggtcg tcgacgagtt cgagaagggc tatacgctca acgggcggct
    21061 gcttcggcct gcccgggtct cggtctgcaa gaagagaacg aacgaaggtg tgtgcgaata
    21121 aacgaatgcg aggcaagcgt catgaaggag gatagataac atgggaaaaa tcatcggaat
    21181 cgatttggga acaaccaatt cctgcgtcgc catcatggaa ggcggggatc cggccgttat
    21241 cgccaatcag gagggcaacc ggaccacgcc gtcgatcgtg gcgtttaccg agagcggcga
    21301 acggctggtg ggtcaggtgg cgaaacgcca ggccattacc aacccggaga acaccgttta
    21361 cgccatcaag cggatgatcg gtcgtaaata cacctcgaaa gaggtccagt acgacaagac
    21421 catctccccc taccagatca ccgaagcctc gaacggcgac gcccaggtga cggtccgcgg
    21481 acggaattac agcccggcgg agatctcctc catggtgctg acgaaaatga agcagacggc
    21541 cgaggactat ctgggcgaga aggtcaccga tgccgtcatc accgtgccgg cctactttaa
    21601 cgattcgcag cgtcaggcga ccaaggacgc cggcaaggtg gcggggctca acgttctcag
    21661 gatcatcaac gaaccgacgg cggcggccct ggcctacggc ctcgacaaga agaaggacga
    21721 gaagatcgcc gtcttcgacc tgggcggcgg gaccttcgac atctctatcc tggagctggg
    21781 cgacggcgtt tttgaagtca aatccacgaa cggcgacacc catctgggcg gcgaggactt
    21841 cgaccagcgc ctgatcgact acctcgccac ggagttcaag aaagaccagg ggatcgacat
    21901 tcgcagcgac cgcatggcgc tccagcggct gaaggaggcg gcggaaaagg cgaagatgga
    21961 attgtccacc tccatggaga cggacgtgaa cctgcccttc atcaccgccg acgcctcggg
    22021 tcccaagcac ctgaacgtca agatcacgcg cgccaaactg gaggccctcg tggaggagct
    22081 catcgaaaag ctcgatgcgc cctgtcggac cgccctgaag gatgcgggac tgacgcccgg
    22141 ccagatcgac gaggtgattc tggtcggcgg catgacgcgg atgccccgcg tccagcagaa
    22201 ggtgaaggag ctgttcggca aggagcccca caagggggtc aaccccgacg aggtggtggc
    22261 cgtgggcgcg gcgatccagg gcggtgtgct ctcgggcgac gtcaaggacg tgctcctgct
    22321 cgatgtgacc cccctgtccc tcgggatcga aacgctgggc ggcgtcatga cgcggctcat
    22381 cgagaagaac acgacgatcc cgacccggaa gagccagatc ttttccacgg cggcggacaa
    22441 ccagccggcg gtgagcatcc acgtcctcca gggcgagcgg cccatggcgg cggacaaccg
    22501 caccctgggg cggttcgagc tcgtcgggat tcccccggcg ccgcgcggca tgccccagat
    22561 cgaggtgacg ttcgacatcg acgccaacgg catcgtccac gtcgccgcga aggatctcgg
    22621 cacgggcaag gagcagagca tcaagatcac ggcgtcgagc gggctttcgg aagaggagat
    22681 ccagaaactc gtccgcgagg ccgaggcgca cgccggcgag gaccagaaga aaaaggacct
    22741 gatcgaggct cgcaaccagg ccgatgccat ggcctacagc gtcgagaaga acatcaagga
    22801 gttcggcgac aagatcgggt ctgatgaaaa gacccggatc gaggaggcca tcgcgaaggt
    22861 caagaagacc atcgaggggg atgacctgga cgccatcaag gccgcccagg aggagctgac
    22921 cacggcgtcc cacaagctgg ccgaggccat gtacgcccag tccacccagc agcaggccgg
    22981 gaccggcccc ggggcgggcg cgggcgccga cgccggcgcc cagacgggcg gcggcaagaa
    23041 ggacgacgac gtcgtcgatg cggacttcga ggaagtgaag tagttttctg gaatcagtca
    23101 gacctgcggg tcgcgtgcct ggaaaccttt tgcggcttcc aggcattttt ttgtatcgac
    23161 gcgagacctg aattcatgta ggactgagat cgtcgggagc cgtaatttct tcccgcaccg
    23221 aacctttgcc cgccgcgcag ctcggcgtac caatcacggg cggggggaga ggggcatcga
    23281 cccacctccc gtgcgcgcat ccgggatgcg atgtccgatg gacgcggcgg acccgttccg
    23341 actgttgcgg aaaaatcgaa cgccgaggag ggccgccggg atgaacaaac ctcttgccgc
    23401 gtggatattc gcggccgttt tggcgattgt catcgctgtg gccccctttc ccgcggacgc
    23461 ctccattcgc ctcctgaagg accgggaata cgtccgcgat ctgatccggg cgatcgacgg
    23521 ggcgcgggaa gagatcgtcg tctgcatgtt cctgttcaag gcgagcggct atcggggcag
    23581 ctatccggac cgggttgtcg aacggctcct caaggccgcg cggcgaggcg tgcgggtgga
    23641 ggtggtcctg gagcggggcg gcgatcccac cggtacggtg gaccgggaca acgaggcgac
    23701 gcgccggcgt ctggagcagg ggggcgtgcg gacgcggatg gactctcccc gccgcaccac
    23761 ccatacgaag ctggtcgtca tcgaccggcg cacgaccttc gtcggcagcc acaacctgac
    23821 ccactcggcc ctgaaaatga accacgaact gtccgtggtg atcgattcac cggcggtggc
    23881 ggccgagacg ctgaagtaca tccggacgtc cgtgctgagg gactgagggt ccctgcggac
    23941 ggcttgagga gtcggaacac catgcgttac ctgttgacca atgatgacgg gatctatgcc
    24001 cgggggctga gcgccctgta tcaggagctc tcccgggatg cggagtgtct gatcgtggcg
    24061 ccggaaatcg agcagagcgc cgtcgggcac gccatcacgc tcctgcgccc gctgatggta
    24121 aggcgggcga agcgggaagg ggagttttac ggatacgccg tgctcggaac gccggcggac
    24181 tgcgtgaagc tgggcatccg ggagcttgtc cgtgaacccg tggacctggt cgtttccgga
    24241 atcaaccggg gggcgaacgt cggcatcaac gtcctatatt cggggaccgt ttcggcggcg
    24301 acggaagcgg ccatcctggg cgttcccgcc atggccgttt cgctcgacac gcatcgggag
    24361 gcggactaca ccgtggccgc gaaggtcgcg cgccgcctgg ccgcctggat gacgaacaac
    24421 ggcctgggga gtggcgtggc cctgaacgtc aacgttccgg cgctgccgga agaccggatc
    24481 cgggggatcg ccgtggcccg acagggacgg gcgcggctga tggagagctt cgagcggcgc
    24541 gtcgatcccc gggagcatga gtattactgg ctggccgggg agacccaact ggccgtcgac
    24601 gaggagcccg attcggatgc ctccgccttg aagcagggcc tggtcacgat tacgccgatc
    24661 gactacgatc tgacctgtca cgaaagcctg gacagactca aggatctgat cgcgaaaatc
    24721 tgagacggca tcgacgggcg gggcgcgcgg cgatagggcg cttgccggcg accggacgcg
    24781 tcacgaaaag cccgggtgga ttcagggacc ggatcgcgag gatccggggc ggcgtcaacg
    24841 ggtgaggagc acggggatgg gactcttccg gatgacggcg gttgtggtgc tgccgacgaa
    24901 gagctcccgc aggcgcccct gtccgtaggc ccccatgacc atcagttcga ccgagccctc
    24961 ctggatgaag cggagcaggg ccttgtcttc cttccccggc agaacgatcg tctgggcgtc
    25021 gatcttgacg tccgccagtg cctcttcgac ctgcttcatg cgttccgcgg ccagggtctg
    25081 attgtcggtg atgaggacga cggtcagcgg ccagccggcc ttctcggaca ggaacgtcgc
    25141 gatcgaaagc gcgttccggg ccggggcgct gccgtcgtag gcgagggcca tgctctcgat
    25201 ctcgcggaag gtatccggcg tcacgacgac cggcttgccc gccttccgga caacagcctc
    25261 ggcggtcgag ccgaggatgc cgccgctccc caggtgaaag tgctcgcccc gccgggccag
    25321 gaggatcccg tcggcctccc ggccggcctc gatgatggat tcgtcgatga cgcccatgac
    25381 cttccgtgcg gtcgccggga tgcccgcctc cccgcagcgc tgctgaaacg ccgcgaggat
    25441 cgtctccgcc ctcgcctcca gtccgccctc gaccgcgggg aggaactcct gatagggcgg
    25501 gagcccgacc gagccggaga tgtcgctgaa aacgggggcc tgcagcagcc ggatatcgac
    25561 gacgtgcaat cccgtgaggg acgcgcccag ttttccggcc atgtacagag cgtactccag
    25621 ggcgatcttt ccctgctccg atccgtccgt ggggatgagg atactccgga tcatgtttcc
    25681 tccttagagc ttttcgtagg ttgagaagtt cttgaggaga tcatcgatga tcttgtcctt
    25741 ttccatccgt tcgtcttcgt tttcctccgt gtcggccgtg tctgccggca gcacggtttc
    25801 gatttcccgc ccggcttcgt cgtaggagac gaatttcccg acgaggcggc cgttccggta
    25861 ggtggccgtt tcccggatgc ggccgttgcg ccagtaggac atgtaccggc cgtcccgctt
    25921 gtcgtttacg aacgcgccct gctccttgat ctgtccgctt ttgtagtacg agacgtaggg
    25981 gccgtcgaac ttgccgtttc tgaagtgctc ttcctccttt aactgaccgt tgcggtagta
    26041 tagccggtag acgccgtcca gcgccccgtt ccggtaatgc tcgctctccc ggatctggcc
    26101 gttttcgtaa tagcagacgt aatcgccttc cagccgatcg ttgacgaagg ttcccttttc
    26161 ccggaggcgg ccgtcgcggt aataggagaa ggcctcacct tcccgccggc cctggtggaa
    26221 ccgcatctgc gccatgacct ggccgttcga gaaataagac gtgtagtcgc cctcccactt
    26281 cttgtccagg atgtggatcc gctctttgag gcggtcctcg tcgtcatggt agattttttc
    26341 gtgcagcgtg ttccgcttgg cgaacatggc cgtcctttac gcgtccgttc ccgtgccggt
    26401 cggcggcgca gggcgccgga ccacgaccgt gcccgcgatc ttgtcgtgcc agccctgctt
    26461 ccgcccgtcc caggcgatcc acaggaatcc caggtacagg aagatgccgg agagaatata
    26521 ccccacccag cgcaggaacg caaggccgaa ggtcattctt tctcctgaga tttgaatgac
    26581 ttgcaatttc atcgccatct tccccggcgt ctggccggtc aggccgtgaa agtaggtgaa
    26641 gtaagccgcg ctcgcgagcg tcgagacgaa caggtacagc cccgtcatgc cccggatcag
    26701 cgcctcgatc gcgtccgggg ttggggagcg gcggggcgag ggcaggtcga cgccgccggc
    26761 cagtacgagg atgagaaaaa ggaagaggga aaccccgtag aggagggcct tgtcgatggc
    26821 gaaggccgcg aagcgacgcc agaagccgcc gtaccggatc gaggtcatgt cccgtcgttc
    26881 cccttttcgg cggtcccgag ggtctcccgt tcatactgca cgatggcgag cgccgccacc
    26941 gccgccgttt ccaccttcag gacccggcgg ccgaggctcg cgatgagaaa cccgtgcccc
    27001 ttcgccagcc ggacctccgc ctccgtgaag cctccctccg gaccgatgac gaccgtacac
    27061 ggccggccct tcaggtccgt cagcccgcgc agcaggttcc ggaggccggt ctcgcgctcc
    27121 tcttcccaga gcatcaggcg gaccgccccg tcttccggcg cggcgacgat gtcctcaaag
    27181 gaggcgatgt cctccacccg cggaatatcc gcgcgccggg actgccggca ggcctcgacg
    27241 gcaacggtct gccagcgggc gaccctctgc cgggccttct ccgccgtgag acgggggatg
    27301 gagcgctcgg aggggaaggg ccggatcgcc gtaaccccca gttccgtggc cttccggatg
    27361 acggcctcca tcgtttcccc cttggggagg gcctgggcga gggttagggg cagggcgtcc
    27421 gcgggaacca cgcggcagac gccgacctga acgctcacgc cgtcccggtc gatgcccgtc
    27481 atgtgggccc gtccctcccg tccctggccg tcgaacagga ccagggcgtc gccctcccgg
    27541 aggcggagga ccgtccgcag gtagcggcgc cagttgccgt ccaggacgca ggtctccccg
    27601 acggcaaagg ggtggggata gtagatgcgg ggtgcggtca aggcgtctgt tccttgtttt
    27661 ggaggacgta gcagacccac tccttttccg tgatcgtccg gcgcgaaacg aacggggggg
    27721 caaaaaagtg cgcctccatt tccggccggt tctgatcgat gattccggac agcaccagat
    27781 agccgccggg cgtcagcagc gacacgaggt gggatctcag gcggatcaga agccgggccg
    27841 tcaggttggc gatgatcagg gaaaagggat cgtgcagggt cgtcacgtcc cgattgacga
    27901 ttttgacccg gtcttcgacc ccgttgatcc ggacgttctc ccgggcgata tccgcggcgt
    27961 ttcggtcgat gtccacgcac acgaccgcct ccgcgccgag cttggcgctg gcgatgccca
    28021 ggatccccgt cccggtgccg acgtcgagga cccgcgcctt ccgaacggag cggttcttga
    28081 gcaggatgtc ctccatcgcc tccaggcaca tgcgggtcga cggatgctgc cccgtgccga
    28141 aggccatgcc ggggtcgata tcgatgacga tgtcgtgccc ctgcggcgtg aagcgttccc
    28201 aggtgggctt gatcacgatg tttttactga cccggagcgg tttgaagtat tttttccact
    28261 gctcgcccca gtccggatcg tcgatggttt cggtcgtcag cgtgaccggg tcgtattcgg
    28321 gaaagagctt ccccaggctc gccaaatacg tttccaggcg cgccaagcgg ttctccaggc
    28381 gcgtatccac gggcagaaag gcgttgagct tgtcgagcgc cgccggccga tcgttcgggg
    28441 caaagggctc cagcacctcc tgataggccc cctgggcgcc gatctccgtc acgtaattgg
    28501 cgagcgcgtc gaccaattcc ggcggcgtcg acagttcgat cttgaaccag cgttcccgct
    28561 tgtctggcgt cattccctgt ttcgccggca tggcggtcct cccttgtttg gccccgggca
    28621 ggcgttgcgt gttgaggccc tgtcgtgttt ataacctttt cccccggact tttcaagagg
    28681 gcgcgggggt tgcctgaatg atgagggaac ctttccccct cccgaaaacc cttgggcgtg
    28741 aggcaacggg gggcggcgac cgggaaaggg cgtggccgcc tcccctagtc gcggtagaag
    28801 cggatcccgt cgacccacag ggagccggcg acgtctccgt tcattttctg gttgaggccg
    28861 atctgaaatc cttccacgcg ctcgagacgc aggacctggt caccgggccg ggccccgtac
    28921 atggccgccg ctccctgctt gatccagccc cgggcgacga ccatctgcgc aaaggggatg
    28981 cggatcctcc cccaggccgg accggcttcg aagaaacccg tccaggcgtc gacccggttc
    29041 gggtcgtcgg gcatggacgt catgaccatg acctgggcat ggaccgtctc cgtcgcccgg
    29101 gcgtcgaacg cgatcccgtc gtagagggac aggtcccgtt tcttgcggtt gtcgatgcgt
    29161 gcccactccg ggtttcttcc cggaacgagc cgatagtcga tccgcatcga tcgcccggaa
    29221 tcggcgctgc catccgcacg gtcgagttcc gtccggaaaa agggcgccgt ccgtcccgtg
    29281 gcgaccatga tccaggaggg gtcgcggacg ggggattcga acgtttcgtc gaaggggccg
    29341 gaatcgcgga tctctgaggc cttcaggagg gccgttttca cgtgaatggc cgacatcgcc
    29401 tgcgaggtcc ggacattgac gagccgcagg tggatgtccc attcgcccgc cacctcggcg
    29461 agggtacccg tcaggagcag atccgccatc atcagctttc cgagaacgtc cccgggtttc
    29521 tcccgggcca gtccgctctg gctgagcgtg agctcgttca tcaccgcctt gagcttttgc
    29581 cgctcgacga cgttgaactg gccggaatcg atcagggccg tggtcaggcc ttccagggtc
    29641 acgcgcccct gatccttctc ccggccgcgg atcacatcga agtccacgat ggcgatcgtc
    29701 ttcttgggga tcgtcaggcg gacggggacg ccggcgggca ggggctcggg tttgtccagc
    29761 gcaaccgtca cggtggcgcc gcgcacctcc ccgacggtcg cgggtttccc gttgacgtcc
    29821 gtcctcatcc cttcgatgat caggctcttc cggatcaggg cctgggcgat ctcgccgacg
    29881 ggtgagcccg gcgtcttgcc catctccggc agcttcattg ccagggcgag gccgtttccg
    29941 gcgggaccct ggaccgttcc ctcgagatac aggacgctcg cgggctccgg cggtgccttt
    30001 gtcggggcaa cggtggcgca ggaccaggaa aagagtgcga gcgcagcggc caggagcggc
    30061 atgacaggct tcatcgtttc acctccgcgg accgggaccc gcccggaaac cggcgacgga
    30121 cgaaagatag gcattctccc acgcccggag tatggccaga atgaaagcgg gtgtcaataa
    30181 aatagtgact tcgacgctgc gccgccgccc ggtcccgcct tgaattttgc gggtcttcat
    30241 gataccttcc caaccggtgt ttcgggcccc ggcttgaatc gtcccgggcg ccgggcggga
    30301 aggagccgat gaactattat cccatctgtc tgaacgttgc gaagaagcga tgcgtggtca
    30361 tcggcggcgg ccaggtggcc gcgcgcaagg tggagcgcct gctcgactgc ggcgccgacg
    30421 tggtcgtggt cagccgcgcc ctcgcgccgg aactggcgga ctggaaggcg gaagggcgga
    30481 tcgttcaccg ggagtccgag taccacgccg cctgtctgga cggggccttt ctcgtcatcg
    30541 gagcgaccga ccgcgaagac gtgaacgcgg cggtctcgcg ggacgcccgg gcccgggggc
    30601 tgctcgtcaa catcgtcgac gcgccggccc actgcaactt catcctgccc tccctgcacc
    30661 agcagggcga cctcgtgatc gccgtctcca ccggggggaa gagcccggcc ctggcgagaa
    30721 aaatgcgtga ggaattcgcg cgtatctacg gctgggagta cggcgtgctc gtcgagatcc
    30781 tgggggagct ccgggagcgg atcatggccc gggggcgtcc cccggatgag aacaaacagc
    30841 gcttcgacgc cgtggtcggg tccgatgtcc tcgaggcgat tcgccgccgg gacgccgaag
    30901 cggtccgcgg gctcatcctc cggctgaccg gcgaggatct gcccgtgtcc ttccgggaag
    30961 gagcggaacc ctgatggaca ttcttctgtt ccgggcggcg ctgtcgctgt atctcgtcag
    31021 ttgtctgggc tacctctcat cacttttcgc ccggcgggtt ctcgtcgcga aatacgccgg
    31081 ctggggcctg ctcgcggccc tcgtcgtcca caccctggcc ttcggcgcgc gctgcctcgc
    31141 gacgcatcag aacccgctcg tcaacatcta cgccgccctg tccttcttcg cctgggtcct
    31201 cgcggccgtc tacctggtct tccaggtgcg gacgaagacg cgcgttctgg gggtcctggt
    31261 tgcgccggcc gtcgtcatcc tgatgatggc cgcctccgcc ggcctcgggg gggcggtggc
    31321 cctgccgccg atcctccggg ggcaccttgt ttcggtgcac gtcgtcctcg cggtgatcgg
    31381 tgaggcctgc ctggccctgg tcgggctggc gggcgcgatg tacctcctgc aggatcgcct
    31441 catccggaaa aagcgggcga cacggctgct ccggctcctg ccgccgctcg aggatctgga
    31501 ccggatcaac caccttggcc tcctgtgcgg attccccctt ctgaccctcg gggtgatcgc
    31561 cggcgcactc tgggcgcgga cggtgtgggg gacgcactgg ccgggggatc cgaaactggt
    31621 ctggacgctc ctggcctggg cgctctacgg gctactcctg caccagcggc tggccctcgg
    31681 ctggaagggg cgccgcatgg ccatcggcgc gctgacggcg atcggctttc tcctggcggc
    31741 cctggcggcg acgggcctgt ttttccagtc ggtgcacacc tttggctgaa acgatgcatc
    31801 tcgttctgat cggcatgaat cacaggaccg cccccctgga catccgggag cgcctttccc
    31861 tgtcctgcgg ggaggagggc cgccccctgg cggagctcct cgcgattccc ggcgtggacg
    31921 aggccttcta tctcaacacg tgcaaccgtg tggaggtcct ggtcgggacg gggacgccgg
    31981 acgcggccct ggcgggactc agggacttcg tcctgcggca cggcaacctg tcgggggaga
    32041 tgctcgagcg ctgcctgtat ctgtatcggg acgaggcggc cgtgcgccac cttttccgcg
    32101 tcaccgcgag cctcgattcc ctcgttatgg gcgaacccca gatcctgggg caggtcaagg
    32161 acgcctatcg ttcctcggcg gaggcgaacg ccgcgggggc gatcctgaac cgggtcctgc
    32221 accacgcgtt ccgcacggcc aagcgcgtcc gaacggagac ggccatcgcg gccaatgccg
    32281 tgtcggtgag ctttgccgcc gtggagctgg cgaagaagat ctttggggac ctccgcggca
    32341 aaaccggcct gctcgtcggg gccggggaga tgtcggagct cgcggcgcgg cacctgctcg
    32401 gccaaggcgt ggggcggatc gtcatcgcca accggaccct cgcccgggcg caggagatgg
    32461 cctcggccct cgacggggag gccgtggggt tcgagcgtct gcccgaatgc ctggaggcgg
    32521 cggatatcgt catcgcctcc acggggtcgc cgggctacgt cgtgacggcg gagatggtcg
    32581 gccgggcgct gcaccggcgc cggaaccggc tcctgttcct cgtcgacatc gccgttccgc
    32641 gggacgtcga gcccgccgcg gcgcggatcg acaacgtcta cctctacaat atcgacgacc
    32701 tccaggggat cgtggacgcc aacctggcgc aacggcgcga ggaggcccgg aaggccgaag
    32761 ggatcatcga cgcggaggtc gcgaattacc tggagtggtt caacacgctg gaggtcgtac
    32821 cgaccatcgt ttccctgagg gagaaggtcg acgccatcct ggagggggaa ctggaacggt
    32881 cgcatgcctg gatgcagcat atgcgcgagg aggatcggga gaacatcggc atcctcgtcc
    32941 atgccgtggt aaacaagatc ctccacgacc ccatcacggg gttgaaggag gagagccagg
    33001 atcacgccgc ccggccctat gtggccgcgc tccggcgcct gtttcatctg gagagctgaa
    33061 aaggatccct cgaagggagg acggacgatc agaaacctca tccgaatcgg cacccgtggc
    33121 agccaactcg ccctcacgca gacgaacgcg gtcgcgaccg agatccgcaa gcggcatccg
    33181 gcgctcacgg tcgaggtgca ggcgatccgc acgaaggggg acatcatgca ggacgtctcc
    33241 ctggcgaaga tcggcggcaa ggggcttttc gtcaaggaga tcgaggaggc cctggtgcgg
    33301 ggcgagatcg atctggcggt tcacagcatg aaggacgtgc ccgtggaact accggcgggg
    33361 ctcgcgatcg gcatcgtgac gcggcgagag gacccgcgcg acgttctggt ttctcgggac
    33421 cggctgaagc tcgaggcgct gccggagggc gcccgtctcg ggacggggtc gctccggcgc
    33481 gggttccagc ttcgcaacat cctgcccgac gtcgagatcg tttcgctcag gggcaatctc
    33541 gacacgcgaa tccgccgggt cgggacggag gggctcgacg gggtgatctt ggcggccgcc
    33601 gggatccggc ggatgggctg ggtcgagcgg gtgagccagt tcatccccgt ggaagtcctg
    33661 ttgcccgcca tcggccaggg ggtgctcggc atcgagcacc ggtccgacga ccgggagctt
    33721 ctggaggcgc tggcctttct acacgaccgc gagaccgcgc tggcggttac ggcggagcgg
    33781 gccttcctcg cggacctggg gggcgggtgc cagctgcccg tcgcggccta cgcacggctc
    33841 cgcggtccgg agctggtctt gcggggcctc gtgggcagtc tcgacggccg tgcgatgatc
    33901 cgcgacgagg tccggggagc ggcggacgac ggcgcgaccc tggggcggag gctcgcctcg
    33961 acgattctgg accggggcgg ccgggcgatc ctcgaggcgg tctatgcgtg cggcgactga
    34021 ggccggtgcc cgccgcgggc gggccttgtc cgcgccggac ggggggacgc aggccctcga
    34081 cggcgttatg gggatggctt aaacgacgga acggacacgg aggcgaaggg gacggatgga
    34141 caagatcggc aaggtctaca tcatcggggc gggtccggga gacccggagc tgatcacggc
    34201 caaggcgatc cgctgcctga aggaggccga cgtggtcatc cacgactacc tggtcagccg
    34261 cgagatcctc cgccatgcat cgacgtcggc gcgcttcatc tacgcgggca agcagggggg
    34321 cgaccacacc ctgagccagg aggagatcaa ccgccgcctc gtcgaggagg cgcgacgggg
    34381 ccataccgtc gcccggctca agggcggcga tcccttcatc ttcggccggg gcggcgagga
    34441 ggcggaggag ctcgcccggg cggggatccc cttcgacatc atccccggcg tcacgtcggc
    34501 gattgccgtg ccggcctatg cgggcattcc cctgacgcac cggggcctga cctccacgct
    34561 cgccttcgtc acgggccacg aggatccgac caagccggcg agcgacatcg actggacggc
    34621 cctggcgaag atcgggagcc tcgtctttct catgggggtc aagaacctgc cggagatcgc
    34681 ggcgcgcctg gtcgaacagg ggcgcgatcc ggccaccccc gcggccctga tccgctgggg
    34741 gacgacgccg gaccaggaga cgctaacggg cacgctcgcg gacatcgcgg taaaagcgca
    34801 tgccgaaggc tttcgcccgc ccgccatcct ggtggtcggc gaggtcgcgg ggctgcgcgg
    34861 gcgcatgaac tggttcgaga caaaacccct gatgggccgg ggggtcgtca tcacccggcc
    34921 cgaggcccag gcggacgaat tcgccgccct cctgaccgcc cggggggcgc gggtccttcg
    34981 attccccacg atcgcgatcg ccccgccgga gcgctgggat gccctggacg ctgcccttgc
    35041 gcggctcgcg acctacgcct ggatcgtttt cacgagcgcc aacggcgtgc gcttcttctt
    35101 ccatcacctg cgggagacgg gcggcgacat ccgtgacctc aaggggatcc ggctcgccac
    35161 gatcggcccg gcgacggccg cgaccctgga ggcgatgggc ctcaaggtgg atctcgtccc
    35221 ggcgtccttc ctctccgagg gggtcgtggc ggcgtttgcg ggacaggacg tcgccgggaa
    35281 ggccgtgctt ctcccgcggg cggccgaggc ccgggacgtc atcccggagg gcctcgcgaa
    35341 aatgggtgca acctgcgatg tcgcgaccgt ctaccgcacg gtccgctccg atcggaagaa
    35401 ggaggaactc gcggcctggc tcgcggacgg gaaggtcgac gtgatcacct tcaccagccc
    35461 ctcgacactg acgcacttca gggacatcat ggggccggaa ttccgcctgc cgcccgcggt
    35521 gcggattgcc tgcatcggcc ccgtcaccgc cgccgcggtg aagtgcgccg gcctgccggt
    35581 cgacatcctg caggagacct acaccatcca cggcctcgtc gccgccctgg aggcgcattt
    35641 ctcgtcgccc tgacgaaccc gggccgatcg ttgcgatccc tccgcagcgg gggagcggtc
    35701 ccgcgagcgt gcacccgttt ccgcccgccg gggcgaacga ttgttttgac atttgccggt
    35761 ggaggctata gagttcggca gaacgttggg gatgacggcg atgatcggga tttcgaaact
    35821 gtactgcggg gccgtggagc ccggagacgt gctgcgttac gggcgcgagt cggggaagct
    35881 gccctcccac ctgctccagt tctccgccga caagaagccg gtcgtggttt ggaacgtcac
    35941 ccgccgctgc aacctccgct gcatccactg ctactccagt tcccgggacg tcgaataccg
    36001 cgatgaattg tcgacggcgg agggccgggc gctcatcgac gacctggccg atttcggtgc
    36061 gcccgtcatc ctgttctccg gcggggagcc gctgatgcgg cccgaccttt cggagctggt
    36121 gcatcacgcc gcggcgcgcg gcgtccgcgc ggtcatctcg acgaacggca cgagaatcac
    36181 ggaggcattc gccgcgcagt tcagggacat cggtctgtct tacgtggggg tgagcctgga
    36241 cggcctcgaa cccacgcatg accggttccg gggccgcgcg ggggcctttg cggaggcgat
    36301 ccggggcatc cggatctgcc gggaggcggg gatcaaggtg ggcgtgcgct ttacgatgaa
    36361 ccggctgaac gtccgggata tccccgccat cttcgatctc ctggaggcgg aggacatccc
    36421 gcgggcctgc ttctaccacc tcgtctattc cgggcgcggc tccaagctga tggaggagga
    36481 cctgtcccat gccgagagcc gggccgtgct tgacctcatc atggaccgga cgcggagcct
    36541 cttcgaccgg ggaaacccca aggagatcct cacggtggac aaccacgccg acgggccgta
    36601 cgtctacctg cggctcctca aggagggtcc ccggcgcgcc gcggaggtcc tggagcttct
    36661 gaagatgaac gagggaaaca gctccggcaa cgggatcggc tgcgtgagct gggacgggga
    36721 ggtgcacgcc gaccagttct ggcgggggat ctccttcggc aacgtccgcc gccgttcctt
    36781 cagcgagatc tggagcgata cctcgaacga actgatggcc cgcctgaagg acaagcgccc
    36841 gcacctgacg ggccgctgcg cgacctgccg ctggctcgac gtgtgtggcg gcaacttccg
    36901 cgcccgcgcc gaggcggcga ccggcgagat ctgggcgccc gatcccgcgt gctacctgac
    36961 ggacgaagag atcgcccgga cgtgagcggg gagacgcgcc ggatgtccgc gcggctttga
    37021 agcccgctca cgctgggagc gccgccgccc gaacacaaga tgcccgaaag gatccgacga
    37081 tgaattttcc ggactatcga ccccgaagga tgcgcaagaa cgagaacttg cgccgcctga
    37141 tccgcgagac gcggctcgtc gcggacaacc tgatctatcc cctgttcgtc gtgccgggca
    37201 aaggcgtgaa gaagcccatc gcctccatgc cgggggtctt ccagatgtcg gccgacgtgc
    37261 tcgtccagga ggtccgggag gcgcgggagg cggggctgcc cgccgtgctg ctcttcggcg
    37321 tcccggagcg gaaggacgag gttgcctccg gggccttcgc ccgcgacggc gtcgtccagc
    37381 aggcccttcg gcggatcaag gacaaagtgg ccgacatcct cgtcgtcacc gacgtctgcc
    37441 tgtgcgagta cacgagccat ggccactgcg ggatgctcaa gaagggcgcg gtggacaacg
    37501 acgtgaccct ggaggtcctg gccgagacgg ccgtctcgca tgcccgggcg ggggtcgaca
    37561 tggtggcgcc ctcggcgatg atggacgggc aggtgggggc gatccgcgag ggcctggacg
    37621 aggcggggtt cgacgacctg cccatcatgg cctacgcggc gaagtacgcc tccgcgttct
    37681 acggcccgtt ccgggaggcg gcggaaagcg cgccgcagtt cggggaccgg aaggcctacc
    37741 agatggaccc ggcgaacggc gacgaggcca tccgcgagat cacgctggac gtggaggagg
    37801 gtgcggacgt catcatggtc aagcccgccc tcgcgtacct ggacgtcatc cgccgcgcgc
    37861 gggaggagtt cgacctgcct ctcgcggcct acaacgtgag tggagagttc gcgatgatca
    37921 aggccgccgc gcagctcggc tggctcgacg gcgagcgggc gatgtgggag gttctgacgg
    37981 ccatccggcg cgccggggcg gatatgatca tcacgtattt cgccctggag gccgcgcggg
    38041 gcctgagaaa ggcgcagggc tgagggcgaa agacgcatgg cggagaactc gggatgaaga
    38101 gccgagatcc cgtggcttca gggctgaggg ttgagggcgc aggcaaaagg caaaagtgaa
    38161 gggcgaaggg cccaaggctt cagggttgag gacccagggg ttaggcctca ggcctcgggg
    38221 caaagggaat cggggaggat cgtttcgggg gcgaggatgg cggactcggt cacgcaaaaa
    38281 aatgcgccga agggcgggga aacggacgca cgcaaagcgc gcgccctccc gatcctgccc
    38341 gccgtactcc ggatggtcgc ctgggaaatt acgcggagct gcaacctgtc gtgcgtccac
    38401 tgccgggcgg ccgcctgccg ggggccgtac ccggacgaac tggatacgac ccgctgcctg
    38461 ggactcctcg acgagatcgc cgccttcgcg cggccggtcg tcatcctgac ggggggtgag
    38521 ccgctgctgc gaccggacgt cttcgagctc gccgcctacg ggaccgctct cggcctgcgg
    38581 atggtcctcg ccacaaacgg gacccgggtc gatgacgccg tcgcccggag gctgatcggg
    38641 gccgggatcc agcgggtgag cgtcagcctg gacggcctcg acgccgtctc gcatgacgcc
    38701 ttccggcagg ttcccggggc cttcgacggt gccctggccg ggatcgccgc gctgcgggag
    38761 gcgggattgg agttccaggt caacacgacg gtgacgcggg agaacctctc ccagctcccc
    38821 ggcatcctcg acttggccga gcggctcggc gccgcggccc accacatctt cctcctcgtt
    38881 cccacggggc gcggcaagga tctggcaggg caggagatcg acgccgaggc ctacgaacgg
    38941 acgctggagt ggttccatga gcagagcggc gcctgcgccc tccagctcaa ggccacctgc
    39001 gccccgcact acttccgcat cctccaccag cgtggaggga aggtgcgggc gtccggcgcg
    39061 tccgacggga gccggcccgg tccgggcccg gacggggggg cggggaagtc cgcccacccc
    39121 ctccacgccg caacccgggg gtgcctgggc gggagcgcct tttgcttcat ctcccacacc
    39181 ggccaggtgc agccgtgcgg ctacctggag gtggattgcg gacaggtgcg ggaacgggga
    39241 ttccgggaga tctgggaaac atcgcggatc ttcacggatc ttcgcgatct gaaccgctac
    39301 ggcgggaagt gtgggcgctg tgaattcatc cgggtctgcg gcggctgccg cgcccgcgcc
    39361 tacgaggcca cgggggatta tctcgcggcc gagccgctgt gcgcctacga accgaaaggg
    39421 gcgaggcgcc cggacgggag aaaggaagac gcatgaaacg aatcatctgg ggatgcggac
    39481 tggcgttgct gctggtcgga gcggtatcgg ccgccggccc gacgttcgag caactggata
    39541 cggaccggaa cggtaaattg gagttgaacg agctggacgc cgccgcggtc aagctcttcc
    39601 gggacgcgga cgtcaaccgc gacggagtac tggaccgcca cgaattcaag cggctccagg
    39661 ggccgtcctc gtccttcgac gacctggacg cgaatcggga cgaaaagatc gatctcggcg
    39721 aaatccggcg ctcggccgcg ctcaggttcg atcgtcacga tctgaatcaa aacggcgccc
    39781 tggacgatat ggagtgcagc ccgaaccgga tgccgacggt cagtccgctc atgtccgtct
    39841 atttttgatg cagggcgttc gatggacgaa ctcgaccgca agattctgaa ccttatccag
    39901 acgaactttc cgctcgttcc cgagccgttc gcgcaggtgg ccgaggcggt cggcagcacg
    39961 gaggcggagg tcttggcgcg cgtccgggac ctgcgggaga ggggcatcat ccggcggctg
    40021 ggggcggtgt tcgacaagga gcggctgggc ttcgcgagcg tcctgtgcgc cgcgcgtgtg
    40081 ccggaggagc ggctccggga gtttgtggcg accgtcaacg ccttccccgg cgtgacgcac
    40141 aactaccggc gtgaccacga ttacaacgtc tggttcacct tcatcgcccc gtctgaggag
    40201 gccctcgccc gggcgctcga cgcgattgag gaaaggacgg gcgtcgagat cctgcgcctg
    40261 aaggcgacga agacattcaa gatcaacgcg agctttgcgc tttgaagtca acgacgggga
    40321 cgagacaggc gaagagcgat cggcaacggg cgcgaagcgg cgggcgtcag ccgtcaggca
    40381 aaagccaaga gcgaaagggc cgcgtcaggc aagggaccgg aggactctga gatgaatgac
    40441 cgaaaccgtc ccaccctggt gctggtggac ggcagcaact atgtcttccg ggccttcttc
    40501 gccatccgcg atctgacgaa ctcccgcggc tttccgacga acgccgtcta cggcttcacg
    40561 accatgctca tgaggctcct gaaggagcac cgccccgact acatcgccgt ggccttcgac
    40621 gtcaaggggc cgaccttccg ccatgaggcc ttcgacgcct acaaggccac acgccggccg
    40681 attcccgaaa ccctcattcc gcagatcccc gtcatccgta aagtcgtccg ggccttcaac
    40741 attccggttc tggagaagca gggcgtcgag gcggacgacc tcatcgggac gctggcaaag
    40801 cgccatgcgg cgcaggggat cagggtcctc atcgtctccg gggacaagga catgatgcag
    40861 ctcgtttccg acgacgtcgt gatggtggac acgatgcgtg acaagacctt cgacgtcgcc
    40921 gccgtgcggg agcgcttcgg ggtcgccccg gatcgcgtgg tcgaggtgct ggggctcatg
    40981 ggcgacgcct cggacaacat tcccggggtt cccggcatcg gcccgaaggg ggcgcagcgc
    41041 ctcctcgagc agttcgggac gatcgaggcg atgctcgccc agcccgagaa gatctacaac
    41101 gcccggatgc gggaagcggt gctcgcccat gccgagcagg ctcgcctgag ccgggagctg
    41161 gccgtcatcc ggacggacgt gcccgtggag ctggacctgg agtcctgccg ctatacgggg
    41221 ccggatgcgg cgaccctgaa ggcgctcttt caggagtacg agttcacctc gctgatcaag
    41281 gagcttgagc tgcgggacgc gccgcaggcc gtggtatccc gtctcgtccg gagcgaagcg
    41341 gatcgggagg cgctccgcgg cgccctgcgc ggcgcgccgg agatcgccct cgacgtcgtc
    41401 ctctccgtgc ccgaggccat gcgggccgag tgggtggggc tcgccgtctc gaccgcgacg
    41461 gaccaggcct tctaccttcc cctgatcgcg cccgaagacg gcgaagcccc cgcgcaggcc
    41521 gccgcggaca tccttgccgc cgtcgcgccg gtcctcaccg atccgtccgt ccggaagcac
    41581 gtccacgacc tgaagaccgc cctgatcgtc ctggaacgga acggcatcgc gctggccggg
    41641 ataggctgcg acacgctcgt cgcagcctac ctcctgaatc cggggcgccg cggtttcgag
    41701 ctcgccgaga tcgcggacga gcatctccaa cggtcgctcg ccgcggcgaa ggagatcgtg
    41761 gggagcggcg ccaaggccgt ctccttcgcg accattccgg cggaaaccct ggcggactat
    41821 gccggggtcc gctcctgcgc cgtccgcgaa ctggccggaa tgctgatggc cgagctcgac
    41881 cgtcaggggt tccgcgacct cttcgccgac gtggagatgc cgctcacgcc cgttctcgcg
    41941 gacatggagc ggcacggggt cctgctcgac gtcgatctcc tccggcggat gtcgcaggag
    42001 atcgagcaac tgatggccct atcggaggag aagatctacc gcctggccgg ggagcgcttc
    42061 aacgtgaact ctcccaaaca gcttcaggtc atcctcttcg aacgcttggg gctgccgaag
    42121 ggacgccgga cgaaggaggg ctattcgacg gatgtggacg tgctcaattt tctcgcgcgc
    42181 agccatgagc tgcccgcgga gatcctcgcc taccggagca tggcgaagct caagtccacc
    42241 tatatcgatg ccctcccgca gctcgtccat ccggaaacgg gtcgcgtgca cacctcctac
    42301 aaccagacgg tgacggcgac cgggcggctg tccagcagca acccgaacct ccagaacatc
    42361 cccgtcagga ccctggaagg gaagcggatc cgccaggcct tcgtcgcacc gccggggtgg
    42421 tgcatcgtct ccgccgacta ttcccagatc gagctccggg tcctggccca cctgtccggc
    42481 gacgaaaatc tcttggacgc ctttgcctcc ggcgcggaca tccacggccg gacggccgcg
    42541 gacgtcttcg gggtcttccc ggagatggtc aacccggaga tgcggcgcca ggccaaggtt
    42601 atcaacttcg gcgtcctgta tgggatgagc gccttcggac tcgcgcggga gctcgacatc
    42661 acgcagcgcc aggcacagct gtacatcgac ggctatttcc gaaaatacgc cggggtgcgg
    42721 gcctatctcg acgccgtgct cgagggggcg cgacgggacg ggtacgtctc gacgctgctg
    42781 aaccgccgcc gctatttgcc ggagatcgtc ggttccaacg cgacggtgcg gcagttcgcc
    42841 gagcggatgg ccgtcaatac ccccatccag gggacggcgg cggatctgat caagatcgcc
    42901 atgatccgga tccaccggcg cctgcgggag gagaggcact ccgcccggat gatcatgcag
    42961 gtccatgacg agctcgtctg cgaggttccg gcggcggagc gggaggccgt gatcgacctg
    43021 gtcaaggccg aaatggaggg ggcgtaccga ctcaaggttc cgctgaccgt ggagatcggc
    43081 ttcggcgcga actgggacga ggcgcacgga ggataaacaa ggagggaaac gatggaaccg
    43141 agaaaggaat ttgcggcgcc ctgcggcctg tactgcggcg tctgcggtat ccggatcgcc
    43201 cacgagaacg acgacacaaa cttcaaggag cgactggcgc cgatctacgg cctgacggcg
    43261 tcggacatcc gctgcaaggg ctgcctctcc gacgacccct tcgtcctatg ccgggtgtgc
    43321 cacatcaaga cctgcacgcg ggaaaagggg tatgagggct gccatcagtg cgcggatttt
    43381 ccctgccggt acatcgagga gttcccgatc gccgtgggga agcaggtcat cctgcgggcg
    43441 attccccagt ggcgggaact ggggacggcg gcgtggatgg ccgcggagga gaagcgctat
    43501 ctctgtcccc actgcggcta caagctcttc cggggagcca agcgctgccg caagtgcaag
    43561 gaacccgtgg cgctggattg agggtgaaga catggcgggc atcatcgaag aggatcgccg
    43621 gccctgggga tattaccggg tcttggccga cgaggcggat cacaaggtca agcggatctg
    43681 cgtctatccc ggcaagcgcc tgagcctcca gcgccaccac cgccgccggg agcactggta
    43741 cgttgtagcg ggcgaagccg tcgtgacgcg cgatcgcgag gagattccgc tcgcggcggg
    43801 gcgggccgtg gacatcccgc ggggcgccgc gcaccgcatc cggaacgccg gcgggacgaa
    43861 cctggtcttc atcgaggtcc agaccggaac gtacttcggc gaggacgaca tcgagcgcct
    43921 ggaggacgac tacggccggg cgtgaacgag ggaccggcga ggatcatcga aaaagggggt
    43981 tcgcgtgaaa cagaccgtcc cgtggaccgg ggtcatcctg gtcgccgtcg tcctggtcct
    44041 gatcgggggc tatctccata tttccaccga cgcggagcgg ctgccggaga accgtcccga
    44101 agtcttcctg gctcagccgg cagcgaaacc gggcgccagg gtggtcgtct gtgccgggga
    44161 cagtctgact cacggcgcgg tgagtgtcaa ttatgtcgag cttctcggcc ggacgttccg
    44221 ggacggcggg tacgaattcg tcaatgccgg cgtcaacagc agcctggcgt acaatctgaa
    44281 caggcgcctg gaagatatca tccggtgcga tccggattac gtcacgatcc tgatcggctc
    44341 gaatgacgcc aatgcgtcgc tcggcgaccc gaccgcgcgg cgactcatcc ggaagatgaa
    44401 actgccccgc cggcccgacc gcgcgtggtt ccgcagcaac ctcgtcgcgg tgtgcgaaga
    44461 actgaaggcc cgcacccacg cccggatcgc cctgctttcc ctgccgccca tcggggagga
    44521 cgtggacagt gtcgcctttc ggcgcgcggc cgaatacagc gggatcatca aggaggtggc
    44581 ccggtcccag aacgtggact accttccgct gaatgaagcg atgaccgccg gtatccgggc
    44641 gcgcgggcaa aaaccgacgc tgtcgcacac gggggacacg cagcttcccc tgtacgcggc
    44701 cctggcgaag cactacctcc tgaggcaatc ctacgatgac atatccgcgg ggaacggatt
    44761 tttgtacctg accgacctcc ttcacctgaa cacgcggggc gcgacgctgg tcgccggctt
    44821 tatcggggag ttcatcacgc ggaaataacg ccggagcgga aaatgagttc atgaagaaca
    44881 taaacatccc cagaatcgat ttccacgtcc atctgtttcc ggaccgaatg tgcgacgcca
    44941 tctgggagtt tttcgccagg gattatcgtt gggacgtcct ccataagctc taccatcgcg
    45001 agtgcatcca gtacctgcgc gaacacggca tcgagagaat cgtctattcc aattacgccc
    45061 atcgggaggg cgttgcggag ggcctcaatg aatggaatgc gcgggtgctc gacgagaatc
    45121 ccgaccttta ctgcttcgcg gcctttcatc ccggggatga acatgccctg gaccaggccg
    45181 aggaaatcct ggatcatccg cgggtgctgg gcttcaaact gcagcttctg gtccagtgct
    45241 tttttccgca cgacgagcgc ctcttcccgc tctacgaacg ggtcatggac cgggggaagc
    45301 gcatcctgtt ccacgtggga accggcccgg tcggcaacgc gtacgtgggg ctgggtccct
    45361 tcaggaagct cctccggcgg tatccggacc tgccggccaa tgtcgcccac atgggcgcgt
    45421 acgagtacca gggcttcatg gatctgctgg acgatcaccc cggcctctac ctggacacgt
    45481 cctacacgtt cttcaaggat tggcagggcc ggggcggatt cgatctcggc aacgcgcccc
    45541 ttgaaaaata caaggaccgc atcctgtacg gttcggattt tccgaacatc atcctgccgc
    45601 gcgagtcgga gatcgaaacg ttgcgggagt acggcctgtc gcgggaattc tatgaccggg
    45661 ttttctacga caacggcctc aaactgatcg acgcgacggt cgcgccgatg gccgaataaa
    45721 cattgacttt tgggctccga acggtgtatg aaacgccccg ttcgcgaaac cgggccggtt
    45781 tgcccggttt tttgcattcg gaaaacgcgc atcggaaggg ttgaatttgg ataggcacca
    45841 tcgacgggaa gtagatcgtc gccgcaattt cgggatcatc agccacccgg atgccggcaa
    45901 gacgacgctc acggaaaagc tgctgctctt cggcggggcc atccagcagg ccggggcggt
    45961 gcgggcgcgc aaggccgccc gccacgcgct cagcgactgg atgggtatcg aaaaggagcg
    46021 ggggatctcc gtgaccacgt cggtcatgaa gttcaactac cgcgactacg aggtcaacct
    46081 cctcgacacg ccgggtcacc aggatttctc cgaggacacc taccgggtgc tgaccgccgt
    46141 ggacagcgcc ctgatggtca tcgacagcgg caagggcgtg gagccgcaga ccgagaagct
    46201 gatggaggtg tgccggatgc gcaacacgcc gatcatcacc ttcatcaaca agctcgaccg
    46261 cgacggcctg ccgcccctga ccctgctggc cgacatcgag gacaagctcc agatcgagtg
    46321 cgtgccgatg tcctggccga tcggcatggg gaagtccttc aggggggtct atgacctcta
    46381 ccgccgggag cttcatctct tcacccccgg cgggtctacg caggcccgcg aggggctcgt
    46441 gatcaccgac ctcgaggatc cccgcctcga cgaacttttg ggaacccagg ccggcgagct
    46501 ccgcgagcat gtggagcttg tcacggaggc cgccaatccc ttcgaactga cgcagtacct
    46561 caagggcaac cagagccccg tctttttcgg cagcgccgtc aacaacttcg gcgtgaagga
    46621 actgctcgac gccttcgtgg agatggcgcc cgccccggtt gcccggccca cgctgacgcg
    46681 ggaggtatcg ccctacgaga cggccttcac cggcttcgtc ttcaaaatcc aggcaaacat
    46741 ggatcccgcc catcgcgacc gcattgcgtt cctgcgggtc tgctccggaa cgttccgccg
    46801 ggggatgaag gtcatgcacc accggatcgg taaggaggtc accctgacca acgccaccat
    46861 cttcatgtcc caggaccgca cgaacgtcga ggaggcctat cccggcgacg tcatcggcct
    46921 gcacaatcac ggcacgatca agatcgggga tacctttacg gaaacggaac cgctcaagtt
    46981 catcggtatc ccgagcttcg ccccggagaa tttccggcgc atccacctga agaacccgat
    47041 gaagagcaag cagctccaga aggggctggc gcagctctcg gaggaagggg ccgtgcaggt
    47101 cttccagccc ctggggaaga gcgactacat cctgggggcg gtgggcgtgc ttcagttcga
    47161 tgtcaccgcc gagcgcctgc gcacggaata cggcgccgat accgttttcg aggagacgcc
    47221 gtacaacgtt gcccgctggg tggcgtgcgc ggacccaaag cgcttcaagg agttcgagcg
    47281 ggaaaacggg agctccctgg ctctggacgc cgagggccgc ccggcgtttc tcaccgccag
    47341 cgagttccgc ctggagcgct gcatggaatc ctggcccgac gtagccttcc tcaagacccg
    47401 ggaatacgcc tgacccctcc caccccttcg gccacgaggc ggaacccctt ttcccgggaa
    47461 tcagacgatt ccggcggcga tgagccccgt ggccagcgtg accgccatga ggacgagggt
    47521 cgtccgcccg gaccagcgat ggatcgtgcg gaggagggcg gcttttcccc ggatgcggaa
    47581 ggacgcctgc cccagaaccg gcgtgagaag cgccgaaacg agtgtcgcca ggcccagcca
    47641 ggtgtgggga gagctcaggt gaccgccgcc cgcccccgcg accatagcga cggcgaacgc
    47701 cgccccgagc caggtggcgc ccgcaccgcc caggcccagc cgccggtgtc cggtcagcca
    47761 tccccggcgt gtccgggcga acctcgccac ggcgccgccg gccgccgcca gcccgaatgc
    47821 cgcaactacc aagcccgcat gcgcgtaaaa ggccgtcacg gtcgcggtcc tttccgcggt
    47881 ccgtttcgga tcgtgaggat cacttcgttg ccttgggaac cgtgatcttt tccgtcttcg
    47941 atccccagat gttgcatgtg accttgacct ccaggatgtc accgccgacc gcggcgacct
    48001 tgtaggcata ggtgaactcc ggggtgcccg gctggctctt gtactcggtc gtttccaggt
    48061 cctttccgtc cttccggacc accacccgct tgatgtaatg gaagccgggg ttgggagacg
    48121 ggtggctgat ggtcacgctg agcgtttgcg cggcggcgtc gtaggcgagc cggacggtct
    48181 tggggccatg ggcgccggct tccgcgggaa gaaaagcggc cgcaacggtg aaggccagca
    48241 gcagccccac gacggtgatc aacggtttca gggtcgtgcg gtgacggttt ttcggatgca
    48301 tctcgggtcc tcctctgttc gggtgtatga gggaaagtcg ccgcggggtt ccgcgtcggg
    48361 tcgcccctac aggtgaatct ccatgtcctg gtgaagcgca aagcattgga gcggcccccc
    48421 ccgttcggaa acgatcttgc ggcagtcgtc caccatggcg tccaccccgt catccgaccg
    48481 ttcctggttg tggtgaaaca gtccgaacat cttcgcattg gcctgaaggg ccaggtcaag
    48541 ggcgtcaccg taccgggaat ggccccaggc ccgatggtaa tcccgttccg tgtactcggc
    48601 gtcatggata agcaggtcgg tgtcccggga gaagtccagg tagtccgccc gggtcaggcc
    48661 gccttcgtgc tggaaagtga gttcgttgtc cgtgaggaaa acgaaggatt tgccgtcctc
    48721 cacgattttg tagcccacgc cctggttggg gtggctcaag gggatcgggg tgatcgtcag
    48781 ggaactccag gtaaacgact cgtcgcaggc ctcatggtag gtgattttac cgcggatgtc
    48841 ctcgaaattg acgggaaagt ggggtgcggt catgatgttg gccagcagtt ccttgatgga
    48901 cgactgggcg aagggacacc cgaacagggt gatgtccgtc ccccgaatgt agatggggcg
    48961 aaaaaaagga aagcccatca gatggtccca gtgggcatgg gtgaacagca tggtatatcg
    49021 gcggcgcttt tcctccagga ggcgatttcc cagccttctc agtccggaac ccgcgtcgat
    49081 gatgaggatc tcatcgtctt tggttctgat ttccatgcac gtggtatcgc caccgtactt
    49141 gagatattcc tggcctgaaa cagggatgga ccccctcgca ccccagcagc gaatcagcat
    49201 gctcgttgcc tccggtcgac gaatcagtgc ataccgcacc cgcgctcatg acgaaaagaa
    49261 gcctggaagc tgttgcgatg aatatttcgc ggcattatgc ccgattctcc cgggatttgc
    49321 aaacatcaaa tccgttgcgt ttacattatc ctgcccaatg cctatattag cttttcatta
    49381 cgcacgaagg gagggtttat gccgacgatc aagatccggg ggatgagctg tcagcactgc
    49441 gtcatggccg tcacgaaggc cctcgcgggc atcgagggcg tgacggatgt tcgggtggac
    49501 ctgaaaacgg gcgaggcgag ctacacggag acgaagcccg tcgatcggga cgtgatccgg
    49561 gggcgcattc aaaaggcggg gtacgaggtt gtctgagcgg agccgcgtgg aaacgacgac
    49621 gattgccatc ggcgggatga cctgcgcggc ctgtgtgcgc cgggtggaat ccgccctgcg
    49681 tcaggtcgag ggcgtgacgg atgtcgtggt gaatctggcc acggcccggg ccacgctttc
    49741 tcatcggctg caatgggccg gcctggaggc cctgcgcgac gttgtgacgg accagggcta
    49801 tgtctatctg ggcgttccgg gtgaagaccg cagcgacccc gttcaggagg agcgggagcg
    49861 ggagatccgg gacctcgcgc gccgctttgt cgtgggggcg gtcttgagtg tggtgatctt
    49921 cgcgggctcc atgacgcagg ggcttcccct gcttcgcgcc gtcccccgtc cggccctgct
    49981 ctgggtgctg ttcctgctca cgaccccggt cgtcttctgg gtcgggagtc gcttcatcac
    50041 cggcgccatc aaggctgccc ggcagaagac gagcgacatg aataccctgg tcgccgtggg
    50101 gtcactggcg gcgtacctct actcggcgct cgccaccttc atcccggagc tgttcgccgg
    50161 tgccggcgtg gccgcccacg tctattacga cggagcggcc atgatcgtca ccctggtcgt
    50221 actcggccgg ctcctggagg cccgggcgaa gggaaaggcc tcgctggcga tccggcggct
    50281 cattgacctg aaaccgaaga cggcgcgggt gatcagggga gacgacgagg tggatgtgcc
    50341 cgtcgagacg gtcctgatcg gggatgtcct gcgcgtgcgc cccggcgaaa cgattcccac
    50401 cgatggcgtc gtactggccg ggagttccat ggtggacgag tccatgctga cgggcgagag
    50461 cctgcccgtg accaaggagg cggagtcgcc ggtctttgcc gcgacactca accgggccgg
    50521 gaccttcacc ttcacggcca cgaaggtcgg aaaggaaacc gccctggccc ggatcatccg
    50581 cctcgtggaa gaggcgcagg ggtcgaaggc cccgatccag cgcctggcgg atcgcgtggc
    50641 cgccgtcttc gtgccggcgg tcttcgccat cgggctcgtg gcgttcgccg tctggactgt
    50701 ttgtgtcccc caagcgggct tcagcctggc gatgctcaat ttcatctccg tcctcgtcat
    50761 tgcctgtcca tgcgccctgg ggctcgccac gccgacggcc gtcatggtcg gtaccggcct
    50821 gggggcggag cggggcatcc tgatcaaggg gggggaaagc ctggagaagg cccataaact
    50881 gacccgggtg attttcgaca agacgggcac gctgacccgg ggcgcgccgg aagtgacgga
    50941 cgtccgggtc gcggacgggg aggatcccgg gcggatcctc caggcggccc tctccatcga
    51001 ggcgggttcg gagcacccct tggggcaggc gatcgtcgcc cgggcgcggg aaaggggcct
    51061 tgccccccgg cccgtggagc ggttcaccgc cctggcgggc ttcggcgccc gggccgtcat
    51121 cggtgcggag acctggctgc tggggaaccg ccggctgctg gaggcggagg ggatttctcc
    51181 cggggggatg gcggacgcgg cccggcacct gacggcggac gggaagacct gcgtgtttgt
    51241 ggtcgagggg aggcggcccg tcggtttgat cgcgctggcg gacgaaccgc gcgccgaggc
    51301 gcgggaggcg gttgccgccc tgaaggcgat ggggctcaag gtcgccatgc tcaccgggga
    51361 caaccgggcc acggcccggg ccatcggcga tcgtctgggg attgcggaca tcctcgccga
    51421 ggtcctgccc ggcgacaagg cggcggaagt ccggaacatg caggcgaagg gtgagacggt
    51481 cgccatggtc ggcgacggca tcaacgacgc cccggccctg tccgcggcgg acatcgggat
    51541 cgccgtgggg gccggaacgg acgtcgccat cgcggcctca gacatcaccc tgatgcggaa
    51601 cgacctgacc gccgttgccg acgcgatccg cctttcccgg cagacgatgc ggacgatccg
    51661 ccagaacctc ttctgggcct tcgtctacaa cgccgtcggc attcccgtcg ccgccggggc
    51721 gctctatccc ttcttcggca tcctgctcaa tcccgagtat gccgcggccg cgatggcgtt
    51781 gagttccgtc tcggtcgtga cgaactcgct gcgtctccgt cgccgctggc ggaaacagag
    51841 tggacctgtc ccgcgggtcc cctagcgcac ctcgaacagc cggctgtctt ttccgaagtt
    51901 catgatcaac ggcgtctggg ggtggccgag ccggccggaa atagcctcgg tcatctcctt
    51961 cgaatagcgt cccaggcctt tgaccgttac cgcgccggat ccgtccttgt ccaccgtccg
    52021 gccgtccttc aatccctcga gaagcgtgtg cgtatacaag ccgttgcccc gataaccgtc
    52081 cagcgccgtc tgaacgctgc cggccgaggc atagatgtgc agccccattt tcttggccat
    52141 caccgacatc cgggcatcgt acaaaccgct gacgatggtg tccacgccgc ccgcatgaca
    52201 ggtatcgaaa atgaaaagct gcgacagggc cttgatcctt ttggacatct ccacgatttc
    52261 gttggagctg atcaggccgg cgccgccgtc cagcctgccg tcgaaaccgc cggtgacgat
    52321 gtagtactgg ctttgcagaa gcagaccgtg ggaggcgttg aagaagatga agctgtcgcc
    52381 cggtttcacc cgcgcgacca gcttttcgat ggtcgccagg atgttttgtt tgacggcctg
    52441 ttcgttgaac aggccggtcg ggtggatgtg ggtcggcctg taaagggtcc ccgaccggtc
    52501 cgccagttcc gacaggaagt cttgcgcatc cttggcggca tatttcaggt tgatggcggc
    52561 atcgcggtac cggtcgatgc cgaccgccag gagatacaga tgaggctcat ccggttcgac
    52621 cgtggatgtg aaacggacgg tgtccatcat gctctggacg gtgttggagg cattgaatgc
    52681 ggtcaggccg atttcgtttt cgccggcaat cgcctccagt tccacgcatt catcaacccg
    52741 gtcgcctttg ggacggacca tgacggcgcc cgggctctgc ttctcccgaa gggtcaggga
    52801 tcgcatgtcc cggtggacag ccccgccgtc cagttgagcc agtttcatcg cggcggtcgc
    52861 gccctgtgcc gcggcctccc ggtagaatcc atcggacttg atgagcttgc cgttttggaa
    52921 caggcgcacc tcgccgatgc cgccgccggt gctccgcacc tgataacaga tcttcacggt
    52981 cggatccccg gtctcgtggg ggaggggtga aaatttcacg gacggaggcg gattctggat
    53041 cgcttcctcg accgtgaggg tgacgagacc cgccagatcc tctcccttga gtttcgccga
    53101 cacgatgtcg gggcggtaga aaacgtcata gaactggtcg atcccgtaaa cctggctgcc
    53161 ccggcggatg ttgaggagtt catgcccccg ggccgaagag ttgtagtacc cctccggcgt
    53221 gatcacgatc cattcgccgt tggtgaagcc gatcgactgg acgacctccc ggccgcttgc
    53281 cacgtcccac aagcggacgg agccgtcccc actgccggtg acgatccgtt ttccgtcggc
    53341 ggaaaagcgg gcgccgtaga cgtgcagttt gtggccctgg aagcccatga cccggcggcc
    53401 ggtggcgccg tccagaagct tcgggacgcc gtcgaagccg gcggtcagaa cgtgacggcc
    53461 gtccggagag aaggccaccg aggaaatggc gatgtcccgc cagacctgca gttgggcgcc
    53521 gctttcgacc gcgaagagcc tgaccgtgtt gtcgctgctc ccggcgagca cccgttttcc
    53581 gtccggggaa aatgccaccg agttcacggg cgcgcgaaag tcgtcgaaaa cccgccgttc
    53641 aaggccggat tcgagatccc agacacgaac ggtcttgtcg cggcttcccg tggcgaggaa
    53701 tcggccgtcc ggggaaaagg cgacggaccg gatgccgccg ccgtgcccgg caaggcttcg
    53761 gatcacccgt ccggttgccg catcccacag ttggacgcgg tggccggccg caccggacgc
    53821 gatccggttt ccgtccggag aaaaggccgc cgccccccga ccgtcgagct gactcatcgc
    53881 ccggcccgat tccacttccc gcagcgtcag cgggccgtcc agccggcact ccagcatggg
    53941 cccacgcgga gagaaatcca gcgaggacac gatgccccgg tcgtcccgga aggccctgac
    54001 gtgtttgccg gtgatgccgt cccagaggtg gatggtcccg tcgaaatggc cggacagcac
    54061 atattcgccg cggggcgaca gggcaaccgc atgaacccag ttgaccttgc cgtccagccg
    54121 gcgcagttca ccgcccgtcg cgagatccca cagggacatg ttccggctgc ccgccagtag
    54181 tcgagccccg tcgggggaga tcgccacgga gaaaaccgaa acctcgccgg ccggcagggt
    54241 cttcagctcg acggcccggt cgagatccca gaccttgacg gttctgtcga aactgccgga
    54301 cacggccgtg cgcccgtcgg gcagaaaggc gatcgaggtc acctcgcccc tgtggccgcg
    54361 caggacggcc cgctcccggc cggaggacac ctcccagtag cggacggtgc cgtccttgcc
    54421 gccggccgag agaaggtaat ggccgtcggg cgaaaaggcc acggcgttga ccgcggcggt
    54481 gtggcccgta aacgtgcgga tctcttggcc tgtggaaacg tcccaaagct tgacggttct
    54541 gtcccagctc ccggacacga gaaaccggcc gtccggcgcg taagccagcg tgcgaacggc
    54601 ggcggcatgc cccacgagaa cgccgatctg cctgcccgtg gcgacgtccc agaaacggat
    54661 ggcggcgtcc atgcccccgg aagcaatgac gcggccgtcg ggagaaaaga cggcccggaa
    54721 aacacccttg gcgtgaccgg ccaggtctcc gaccttgctc ccggtcaccg gatcccagag
    54781 aacgatccgt ccctcggttc cacccgacag cagccggcgg ccatcgggcg agaaggccac
    54841 cgacgaaacg taaccggcat gttcctccgc aaaggtgcgg atctcgcgcc cgctggcgac
    54901 atcccagagt ttaaccgtct tgtctccgcc ggacaggatg gattttccgt cggcggaaaa
    54961 ggccgcggac tgcacgccgg aatggcccag ctgcacgaag atctccggcc tttcctgggc
    55021 tggagcgaaa tccacggaca ggccgagcag ggcgagcgtg attgccgccg cgcgtaaaaa
    55081 cgttttatgg aggatcaagc gcctcaaagc gggacgtcag gccttctcga actgggactt
    55141 cccggccccg cagacggggc aggtccagtc gtccggcagg tcctcgaagg gcgtgcccgg
    55201 cgagatcccg ctgtctgggt cgcccttttc cggatcatat tcgtagccgc agaccgagca
    55261 gacgtagcgg gccgcctttt caggagccgg cgcggggggc ggcgccgtgt cggccacgta
    55321 ggtgggggcg gtcttggggg ccttgccctt cttgacctcg tggtagtagg cgtaggtcat
    55381 ggggacgccg tcggccagga tctcgcagtc gacgatgttg cccagaaaga tcgtgtgggt
    55441 gccgcagtcc atctcctgaa cgacctccgc ctcgatgtag gcgacggcgt ggtccaggac
    55501 gatcggagcg cccgtcaccc ccgtcttgta gcgggtcgtc ccgaacttgt cgacgtcgcg
    55561 gccggacttg aagccgaaca ggccgatcag ggtcatcggt gtgtcctggg ccaggatcga
    55621 aacgctgaag caccggcttt cacgaatgaa ggcgtgcgtc agattttcct tgttgatgct
    55681 caccgccagc gtggacggga cggacgtcac ctggatggcc gtgttggcga tctggccgtt
    55741 gaagcgatcg ttctgtttcg aactgacgat gtacaggccg taactcagtt tgaaaaaggc
    55801 ttgtttgttc ataccggcgt ctcctttgca ccatgatgcc cgtcttacct tcatcgcccg
    55861 cctcattgca agaaaaaatg cagttaaaag cgggatatgg cgggccgcgg cgacttttgc
    55921 atggcccttt ggcccttttt ggtatataga aaagttcctc tggatttccg atgggatggc
    55981 gttttggggg cagccgggaa ggagacgatg gtcacggtca gaagcggccc aggccggatc
    56041 gccgcgggtc tgatgcgcat ccgatatgtg cgcgaggccg tcgcggagaa agcggatctt
    56101 tcgggtttat cgagccggcc gacgctccgg acctggaccg gcctggggat gatggcgttc
    56161 agttacgtga tcggctggcc ggcggtgggg ctcctggtct ggctctcgta ccgtctgcgc
    56221 gaacccctcg tggccgtggt cggcggaacg ctcgcctacg gcctgtccca tctcgttttt
    56281 atggccggcg ccgccctggc cggagtggat tacgcgaaac ccgttctccg ctgggcggtg
    56341 cgaaggctgg tggagcgact gaacggccgg gacgcggtcc cggaatcgga ggagacaaac
    56401 gatcgtccgg ggaaaacggg cgttgaacac ccctgagaaa ggaacaggaa tggaaggatt
    56461 cattgcggag ttgaaagaca agatcatcga aaccctgggg ctgacggatg tccgccccga
    56521 ggatatcccc gtggacggcc agctcatcgg gagcgagctg ggactggatt ccatcgacgt
    56581 actcgaattg gtcatgatga ttgaaaagga ttacggcgtg aagatcgaga accgggaggt
    56641 gggggtgaag gtgttcgcgt ccctgcgcgc cctggcagcc tacatcgacg cgaatcgacg
    56701 ggagaaggac cggtgaacgg tgtccgggcc ttcgtctgtg ggacggggat cgtcagtgcg
    56761 gcgggcgaag gggtcgaggc gacctggggc gccctgcggc ggggaacgtc ccgcctgacg
    56821 ccgctggcgc tgttcccggc cgcgcacacg ccccccctgc cggtcggcga ggtccgcgac
    56881 gttccggagg tcgacggcct gcctcgtacg catgccttgg cgtggatggc cgtccggcag
    56941 gtcttggccg ccacgaccct gaccccggac gccctcgtcc tgggcagtac gacgggcggc
    57001 atgccgacga cggagcgact cctggaagcc ggagagcgac ggccggaggc gtaccggcat
    57061 cacgccgtcg gctccgtggc cgcctggctg gcccgccggc tgtcgtgccg ggggccggtc
    57121 atcaccgtct ccaccgcgtg ctcctccgga accgccgccc tcaagattgc cctggagctg
    57181 atccgctgcg gtcaggcccg ggtcgtgctg gccggcggcg ccgacgcgct gtgccgcatg
    57241 acctactacg ggtttcacgc cctgcaactg atcgatcccg ccggcgcccg ccccctggat
    57301 cgcgaccgca gggggctgtc ggtggccgaa ggcgcggcct tcgtcctgct cgtcgcggcg
    57361 gaatccccgc ccgtcggcgc cctcgccgaa ctcgccggag cgggatggtc ctgcgacgcg
    57421 taccatcccg cggccccccg accggacggg acaggcgcgc tcgcggccat gcgggccgcc
    57481 ttggcggatg ccggcatgac gccggcggcg ctcgattata tcaatctgca cggtacgggt
    57541 acgccggaca acgacctggc ggaagcgcgg gccgtgcggg ccctctgcgg ggacccgccg
    57601 ccggtctcgt cggtcaaggg cctcttcggc cacacgctgg gggcgtccgg cgccatcgag
    57661 gcggtcgtgt ccgcgctcgg catccaccgc ggcttcatcc cgggcaattg cggctgccgc
    57721 attccggata cggcgctcgg tcttttgccg gaacttgccg ggcaatcccg cccggtccga
    57781 tcggttctgt ccaactcctt cggattcggc ggcaacaacg cctcgctggt tctgacggcg
    57841 ccgctgtcgg cggggcccgc tcgcccgccg cggccgctcg cccggttttc cgtcctgggg
    57901 accgcctgtc tgacgggggc gggtcccctg gaagagacgc tcgcgcggta ctggcggggc
    57961 gagccgttct cggggcgccc ggaccttgcg gcggtccgga cggagatccc cgcgggacag
    58021 atccgccgcc tcaagcggct gtcccggatg gtgctgctgc tggccctggc ggcccgacag
    58081 gcggcacggc cgccggcggc gcccggcggg atcttcttcg ggaccgcctg ggggtcgctg
    58141 acggagaccc acgactttct caagaaactg ttcgactccg gcgagcggtt cacgagcccg
    58201 acggattttg tcggctccgt tcacaacgcg ccggccagcc aggcggccat ctttctcgag
    58261 gcgacggggg cgaacgtcac cgtgagcggc ggcgaccact cgttcgagca ggccctgtac
    58321 acggcggggc ttgtcctccg tgcgggcgat ccgccggcgc tcgtgatcgg cgccgaagag
    58381 gatcacgacg tcctgtcccc gtgcttcgac gccgcggcca cggcggacgg cggcggcgcc
    58441 ttcctgctcg atccgcgggg cggagggacg ggtccggatc tcgtaccggt ccattttgcc
    58501 ttcaatcccg ggggagacga cggcctcccg gccctgatcg aaagtctggg aggagcggcg
    58561 cggatcgcgg agcggttcgg cgccgtcttt gtgggcctgc cggcggggga ccggtccggc
    58621 ggcgaggccc agctcgagcg cttccgccgg gaaaccgcgt tttcgtcgcc cgtcgtcgac
    58681 acccgccgat ttaccgggga gtttgccggt gcttccgccg tggccgcggt actcgccgtc
    58741 gaaaccgtcc gccggggcgc tctgcccccg gccctggcgg ggggggaaga tccccgcctc
    58801 gggggaaggg ggatcctcct gctcggcctg ggcgaattca tcaccgccat cgaggtgtcg
    58861 gccccatgaa ggtcctgctc atctccgcca acgttaccac caacccctat cccgtctatc
    58921 ccatcgggat cgagtacgtg gccggtgcgc ttgcgccccg gcacgaggtc caccttctcg
    58981 acatgaacgt ccccgacggt ccggacccga tccgccgcgc ggtcggtgaa tttgccccgg
    59041 atctcgtcgg cctttccctc cggaacatcg acaacgtcga tacggtcaat ccccaaagct
    59101 acatcgacgg ataccgcgac ctggtcgagc ggctccgggc atgcacaccg gctcccctcg
    59161 ttctgggcgg cagcgggttt tccatcttcc ccggcgagct gatggcgctc ctcaaggccg
    59221 atttcggcgt ggtcggcgcc ggggagacgc tcgaacggct gatcgaggcc ctggaaaccg
    59281 gggcggaccc gtcggcggtg ccgggcgtcg ccgttcccgg gggcgagatc cccgagcccg
    59341 tcgtctggac gaagacccgc gcgcggcgga ttcaaccgcc gccggcgctg ctggagttct
    59401 atctgaagcg gggcgggatg ctgaatctgc agacccagcg ggggtgcccg tatcgctgtc
    59461 tgtactgtac ctacccgcat ctcgacggtt gccgtccgtg cccggacgat cccgaaacag
    59521 cgggcgagac cgcccgccac cttcaggacc tgggcgcggg gtacctcttc atcaccgatt
    59581 ccgtgttcaa cgccgattgg gagcacagcc tctcggtcgc ccgcgccttc cggcgccacg
    59641 gcgtgaccgt cccctggggg gcctttttcg ccccgacgcg tccgcccggc gggtattacg
    59701 aggccctgag ggaggccggc ttgagccatg tggaattcgg cacggagtcc ctttccgacg
    59761 cgatgctcca tgcctaccgg aagccctttt ccgtggccga ggccgtcgcc gcgcacgagg
    59821 ccgtccgccg ggccggcctg cacatcgccc attattttct cctgggcggt cccggggaag
    59881 acggccggac actcgacgag acgctgggca acgcggagac gctggagagg gccgtattct
    59941 tctttttctg cggggtccgg atcttcccct tcacgccgct ccacgagcgg gcgctggcgg
    60001 aagggcagct cgcgccgggc cagaatttgc tgacgccggt tttctaccgt tcgccgggga
    60061 tcgccgcgcc ggacatcatg gcccggatcc atcgacaggc cggcggccga aacaactggg
    60121 tgtacgggga cggcggacgg gagtcggagc ggatcgtttc ccgccttcac gcctacggcc
    60181 atcccggacc cctttgggag ctgcttctga gatgaaaggg accggaccgg accgcagggg
    60241 gaggggcgcg tgctccccgg cccacgttac cggaggggcc gccttcctca cggcgggtct
    60301 gctcgggttt acccccgttc cggaggcggc cgccgttccc ctgggtgtct tcgtcctcgc
    60361 gtgtctcgcc gccccgttct acccgcggtc gcggttcttc ctccccgtca tcagccgggg
    60421 gaagcgcgtt gaccgggcgg tggccctgac cttcgacgac ggcccggacc cgcgcgtcac
    60481 cccccgtctc ctggacctgc tggagcggca cgggatgccg gcgacgttct tcgtcgccgg
    60541 gaacagggcg gaggcctttc cgggcctggt ccgggacatc cttgcacgcc atcatgccgt
    60601 gggaaatcat tcctaccggc acgatccctt cctgatgctg cgttcccgaa gccgcctccg
    60661 ggaggagatt gcccgcacgc aggcgctcct atccccctta ggcgtccgtc cgctcgcctt
    60721 tcgtcccccc gtcggcatca ccagcccgcg gctccccggg gtcctggagt cgctcggcat
    60781 ggactgcgtg acattcagct gccggggggg agacttcggc aatcggcgcg tcgggggctt
    60841 ggcgcgccgc gtcctgaggc gggtccaccc gggggcgatc gtcctcctgc acgatgtcgc
    60901 gccgtccgca tcggcggggg ttccaggctg gctcgaggag gtggagcgga tcctgaccgg
    60961 cctcgaggcg aggggatatc gggttcgccc cctggcggag ctgatcgggc gcccggtgat
    61021 ggagcgccgt ccgccgtccg accgcgattt ttcttgacac cgttgccgct ggacgttact
    61081 taaaggccac gtcgaaacaa tcggttccgc cccgtcgccc gggcatgatt ccccgctccc
    61141 acgggaaaag gtcgacacac accgtgcggt ttcgaggggg agagacgtcc cagttttccg
    61201 cagagaggct catgagcaga gaagaagttt tcaacctgat ccgggagttc tttgtagaag
    61261 agttcgagat tccggatgag aaggtcatcc cggaggcgga tctgttcgag gatctggggc
    61321 tggacagcat cgatgccctc gatatggtcg ggatgctgga ggcgcgtctg gatatcgagg
    61381 ttctggaaga ggagctgaaa aagatccgga cgatcgggga tgtcgtcgac tacatctcgg
    61441 tcaaggtggg cgatcggggg gacgcatgag aagaccgttc cgatacgagc tttccctctc
    61501 cccgaacgcc ggcggggacc gcattgccgc ctcggcacgc atcccggccg attccgcttg
    61561 gtttgacggc cacttccccg acaatcccat cgtccccggt gtcgccctta tcggcctggt
    61621 ggaggaggcc gtcctccggt cggagcgcga tcggggccgg acggtggaaa tgacgggcat
    61681 tcggcgggtc cgctttcgtc tccccgtcaa gccggacgat ccgatcacgc tgcaggcggt
    61741 tcgggatgtg aagcgcggga cgccggcgtt ctcgttcaac gtctgcttgg aaggggagat
    61801 cgcctgcagc ggcatcctgg cggtcgccga ggcggcgccg accgacgaac acggtccgct
    61861 cgcatgacga ttcgcgtcga tcaccttttc aagtcctacg gcgccaacct cgtgctgaac
    61921 gatgtcagcc tggatttccg cgagggcgcg atcaccggcc tgctggggcc gaacggcgcc
    61981 ggcaagacga cgctcgtctc gatcctcatg ggaatcgtcg ccaaggaccg gggtcgggtc
    62041 ctgattgacg gcctcgacct cgacctcgac cgcgaccgga ttcaggcccg cgccagcatc
    62101 gtcccccagt ccttcgcctt ctaccccacg ctcaccgccc gtgagaatct ggagtatttc
    62161 ggcgccctgt acggcttcaa ggggcggcgg ctcaaggccc gcatggacgc ggcgatcgac
    62221 gccggcagcc tgcagtcctt tctcgggaaa cgcgccgcgg aactttccgg gggcatgaag
    62281 cgccggctca atctcgccat cgggcttctg aacgaacccg ccatcctgta tctggacgag
    62341 ccgaccgtcg gggtggatgc ccagtcccgc agctacctcc tggaccggat cgaccggatc
    62401 aaccgggagc gggggacgac gatcctctat gcctcgcatt acatggaaga gatcgagcag
    62461 atctcccagg acatcgccat catcgacggc gggcggatca tcctgcacga ccggaaggaa
    62521 gacatcctgg cacggcgcgg ggcggtcacc ctgaccctgc agcatctgcc cgggtcgctg
    62581 aaggaggcgc tggaccgcgt cgcgggcctg cgggtggagg tggagacgct ggtgctcgat
    62641 cgcaacgaag acttgaatcg gaccctggct ggcctgttcg acgtgctggc ccggcacggg
    62701 gccgaggtca gggcgctgaa ctgggggaac gggcatctgg aggccctgtt tctggaactg
    62761 accgaacgga cgctgcggga cgaatgaacc gaatctacca cctcctgaaa aaggaattcc
    62821 tcgtcatcag ccgcgatgtt catgcggtgg ccgttctgtt cgtgatgccg atggccttca
    62881 tcctgatcat gtcgctcgcg atgcaggacc tcttcaccac gcagtcgccc gtgaggattc
    62941 ccctgctcgt ttccaaccgg gacggtggaa agccggcgac ggccctcctg aaaacccttc
    63001 gggagaacgc gtcgttcgag gtgaaggaca ttccggcaac ctggtccgac gcggaggcgg
    63061 ccgccgcgct caaagacgat gacgccaaat ttgtcattct gatcgaggat catttctcgg
    63121 cggtcgtccg ggaccgcaag ccccgtacgg gaaggaagcc cctggtcgtc ctcgtcaacc
    63181 ctgtcgtggg gacccagatg cagctcatcg tccagtccgt cctggccaac gcgctgggga
    63241 aggtccggat cgaggtcatg acgaaagaga tgtcccccct gctggagctg gcacggatcg
    63301 acccccgcac gttccgcagg ccttcggagg cgcacatcga gatgcggttc gtgacgcgcg
    63361 gggagcagcg attccgggcg ccgaacgccg tccagcagag tgtgcccgcc tggctcgtct
    63421 tctcgatgtt tttcatcgtc attcccattt ccaacacctt catctcggag cgcagccagg
    63481 gcaccctgca gcgcctgcgg agcattcgcc tgtcgcgggg gatcctcctg ctcggcaaga
    63541 tgctccccta ttttttcatc aacctgatcc aggtcgtcct gatgcttctg gtcggcatgt
    63601 ttctcgtccc ggccttgggc ggatcggccc tgaccctggg ggattccccg gccgggctgg
    63661 ccctcatcgc ggccgcggtc agcttctcct ccatcgccct ggccctcctg atcgccgtgg
    63721 tggcgcgcac gaccgagcag gccaccacgc tgggggggct cttgaatatc gtcttcgccg
    63781 cggccggcgg catcatgatc ccgaagttcg tcatgccggc ggtcatgcgg aaggtggccg
    63841 agctgtcccc catgtcctgg gggctcgagg gattcctcga catcctgctc cgcaacggcg
    63901 gagtccgcga cgtcctgccg gagtgcctgg cgctcgtggc gttcggggcg gtcatgctgc
    63961 tgctgacggc cgcgattctg aaaaaacagt ttgaaggaag cgtttcccca tgaaaggatc
    64021 cctcgacaac aacctcaaga tggacctgaa gcggctcatc atcgatgcct gcgacctcga
    64081 tgtggaggcg tcggacatcg atgacgacgg tcttctgttc ggccgcgggt cccagttggg
    64141 cctcgattcc atcgacgccc ttcagatttc catcgccgtg cagaaatacg gcgtcaagat
    64201 caccgacagc aaacagatgc ggcgggtcat ggcgtcgatc aacacctttg cggattttat
    64261 ccggccggag tgagacggtg cgaaacaggg ccatgatcaa gggacgggcc atcgcgtgcg
    64321 ccctcggcga agatctcgac gccgtcgtcc ggggcgcccg ggagatgcgg cccgggatcg
    64381 cgaggcttcc cctcaccctg gcggggatgg atgaggtccg cccgtactac ctcctttcgc
    64441 ggcgcggccc tgagtccctg agcgcggccc cggagacgtt cttctacgag gtcctgttcg
    64501 acacggttga acgggcgctc gcggatgcgt cgctccggac cgccgacctg cgcgacctcg
    64561 cgattttttt cggctcgacc gccatcgaca ttcccgtcta cgagcacgct tacggacaaa
    64621 ccctccggga aacgtcccgg ccggcgtcca tggtgtcctc ggggtacggc cggatcgcgg
    64681 acgaggtggc cggacggttc ggaatccgcg gaccgaccta taccttcacc acggcgtgca
    64741 cctccagcgc caatgccctg ctgtacgccg cggcgatgat cgccgcgggc cggattccgc
    64801 gcgccctggt catcgggtac gacctcttca gccatcttgg tttttacggc tttgaatccc
    64861 tgcgcctcat cgccacggac gggtaccgcc cgttcgaccg tcggcgcagc ggcatcatca
    64921 tgggagaggg ctgcggcgcc gttgtcctgg aggcggatga cggcggcgac gccggcgccg
    64981 tcctgtgcgg cggcgccaac cgggtggatg cgtacaatgt gaccacccac gcgctcgagg
    65041 gaggcgccat cgcccaggtg atgtgggacg ccttggagga cgcggatgtg gaggcgggcg
    65101 cggtcaccgc gatcaaggcc catggaacgg gcagcgaaaa caacgaccgg atggagggca
    65161 acggcatggt gcgggtcttc ggcgacgccc tgccgccttt tacggttttg aaaccgctga
    65221 tcggccacac cgtcggcgcg tcgggcgtcg tcgagctgat cctcttcacg gcgtgcctcc
    65281 agcggggatt ctgcccgccc accctcggtt tcgaggaggc ggacgaggcg ctttcgctcc
    65341 gtcccgtcac ggagcccaag cccctgtcgc ggggggtctt ccttcttaat ttctttggtt
    65401 ttggcggcaa ctgtaccgct ctggtcgttg ccgtgcgggg gtaagggaca tgttcatcca
    65461 tgccgtgtca cagttcgtcg cgctccccgc ggcaaatccg cggaccttaa aggaggttct
    65521 caaggggtac acggacaccc cgttccgccg cgtcaacaag ttcatcctcc tggcgctggt
    65581 cggcgcccat cggtgcgtgc gggggcggac gctgaacggg aatgcgtcgg tttacctgac
    65641 gacggagaac ggcaatctgg gcgatacgaa caacgtcctg gaccagctct accggcagca
    65701 ctccctcccg atgcccttca gcttcatcaa cacgatgagc aataccgcgc cattttacgt
    65761 cgcccagagc ctgaacgttc tggggcggaa cctctcgatc tccggcc
//
LOCUS       DAOOLJ010000010        54729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_400_length_54729_cov_17.539287, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000010 DAOOLJ010000000
VERSION     DAOOLJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 54729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54729
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_54729_cov_17.539287"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLW16_03425"
     CDS             <1..488
                     /locus_tag="PLW16_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB69990.1"
                     /translation="VNDLADAHGLALERLALPSPPGPTGAVVGDTPSSPGQGGRAVPG
                     TAGESGGATPASHNEDAVTGIRAGEGRVPEEAEGGAGGRALVMNCGYGHGFSVSEVVA
                     AAKRVTGVDFPVVYEGRRAGDPPALIADSTRLRRLTGWTPRHDDLDFIIRTAWDWERK
                     R"
     gene            complement(519..2426)
                     /locus_tag="PLW16_03430"
     CDS             complement(519..2426)
                     /locus_tag="PLW16_03430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB69991.1"
                     /translation="MTEGTQKEAWRDKVVTPGEVLEKIQPGMSIFLSTGMAEPRTLVR
                     HLMASDAANLQDLEMIQLVSLGDTVSIDARYTRKYRLKTFFSGWIAADAITEGRVDLI
                     PSRFSRVPWLFKSGAIRVDAAFIQISPPDANGYACLGVGMDVARQAMERATLRVGEIS
                     EDVPFVLGDTLVRIEDFDYLVHSTEPMIYIDPWPLEDVFAKIGKNIASVIPDGSCISF
                     ALGPIYQGTAQALTRKKNLGIHSPFITDALMCLVKSGAVTNRFKTLFRGKSVGSYAMG
                     TRALMKWLDRNPLIEFQPLDVITDPKNIGQNDNFIAILPARKADLTGDIALHIGKGNV
                     TAGPGAVQELFMGATISKNGRTLFALPSRNRGGESNIVLSTADYPFQFSNRESLDMIV
                     TEYGVAYLAGRTVRERAQALIDIAHPDDRAELVRQAKAARILYADQIYLPESGHLYPD
                     KIARSHIFKNGLKVRFRAIKPSDEDEMRRLFYRFSDQAVYYRYFSPIKTMPHGKMQEY
                     VNVDYRRVMSIVAVLAEAAGERIIAEARYVRRDDLRTYADTAFIVDEAYQNYGIATYL
                     FKMLIEIAQSKGIEGFTADVLVANKAMMKVYEKAPFPIKATLSAGTYELHIPFETASK
                     PATACDPWSIK"
     gene            complement(2943..4712)
                     /locus_tag="PLW16_03435"
     CDS             complement(2943..4712)
                     /locus_tag="PLW16_03435"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB69992.1"
                     /translation="MTYRDRRRAEALLAAETGTVRKVWGRTFTVCLVYPNVYRTGMAN
                     LGFQIVYRLINASDAFLCERLFLPDPVKRGSPSPPFLAKTGRASPDIPGATAPLSLES
                     RKPLNAFDIVAFSLPFENDYPNILKILDGGRIPLAAADRAEADPLVIAGGIAVTLNPE
                     PLADFFDLFLLGEGEEMIPEFLATWAAGRERGDDRLERLIRIQQDVGGAYAPALYAAR
                     YGETEGKRCIASSFPRRRDPGLLKEGPDSRLIGDVPAVIRRRHVADLDRHPAEQAIVT
                     AGQEFGEMYLTEVSRGCGRGCRFCAAGYVYRPPRFRSAATLAPAFARGIDAGKRIGLL
                     GTAVSDHPELPALCRAILARNGRWGVGSLRIDRLDDEMVGLLKETGVDTVALAPEAGS
                     QRLRDLIRKGISEAQILAAAGRLAAARIPNLRLYFLVGLPTETDVDIEAIVALTRRIL
                     HAETETGIEGGAGPAVRSGTARRAFRRVTLSLNPFIPKAATPWQRRPLADIRATADKI
                     RRIAAALRPERAVRVIHDTPRAAYLQALLSLGDRRVGAILLAAHRNGGNWPRAFKEAA
                     VHPDVFVYGPKPPGAPLPWSHIK"
     gene            complement(4867..6012)
                     /gene="ftsZ"
                     /locus_tag="PLW16_03440"
     CDS             complement(4867..6012)
                     /gene="ftsZ"
                     /locus_tag="PLW16_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB69993.1"
                     /translation="MFVLSDENAVHAARIKVIGIGGGGGNAVNTMISYNLQGVDFIAA
                     NTDAQALRASLSPIKVQLGAEVTKGLGAGSDPETGRQAAEETRDALQEHIGGADMIFL
                     TAGLGGGTGTGGAPVIAQVAKEQGALTVAVVTKPFHFEGKRRSAQAEEGIEELREIVD
                     TLIVVPNQRLLSLGGRNISLLDAFKKADDILYHAVKGISDLIVIPGLINLDFADVKNI
                     MSEKGLALMGTGIAGGENRAVEAAQKAISSPLLEDNSIQGARGVLLNITGGPDMTLFE
                     IDEASSMIQQEAHEEANIIFGTVVDETMTDEIRITVIATGFEDTIGKKAIGKVASMGG
                     YKRADLATPTFMRNEKAEGLNVVKMGLIDDEANPDLEIPTFLRRQAD"
     gene            complement(6025..7251)
                     /gene="ftsA"
                     /locus_tag="PLW16_03445"
     CDS             complement(6025..7251)
                     /gene="ftsA"
                     /locus_tag="PLW16_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPB69994.1"
                     /translation="MGKKGNVIVGLDIGTTKTCAIVGEMTDGGIDIIGIGSCPSEGLR
                     KGVVVNIDSTVESIRKAVEEAEKMSGCEISTVFAGVAGSHIKSQNSLGIVAVKGREVG
                     EEDVDRAVEASRAIAIPVDREILHTLPQSFIVDDQDGIRDPVGMSGVRLEAKVHIVTG
                     ATTSIQNIAKSISRVGLEIHGLVLEQLASAEAVLGPDEKDLGVALIDIGGGTTDIVVY
                     AEGSVKHTAILPVGGNYVTSDIATGLRTPVTEAEKIKIHYGCAYTPLIPKDEAIEVPS
                     VGGREPREVSRQILGRIIEPRMEEILNMAMKEITKSGCDEMLTAGVVLTGGTALLHGM
                     PELGEQIFNLPVRRGLPTGVGGLKDVVASPMYATGVGLLLYGSQNLDREVLVRIDRSV
                     FGNWIGSLKRWFLEFF"
     gene            complement(7267..8127)
                     /locus_tag="PLW16_03450"
     CDS             complement(7267..8127)
                     /locus_tag="PLW16_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPB69995.1"
                     /translation="MGWLKKHKPFNNRLEARKYRLQRRSGTLARDAIYAFLLLGAVFM
                     ITAFAAYIYLFVVSAPWLVIRETSVRGVHELTEKDVLALAAVKPRQNLFAVNCTAVAR
                     RIRTNPWVRDVFVGRELPGRLVLIVRERRPVAMVKQGPDLFLMDTDGVPFKRWDTGDE
                     ADFPVLTGGGADGRMPTELTARAVALLDELARTRDLAPYGAVSEIHGSTVTGFTVYTD
                     AGLCFRLGFDNYGNKFKRLAPVMADLEKRQIKPAYLLIDLADPTKITVQPRRLPGPPA
                     PAEPAQAYRT"
     gene            complement(8344..9441)
                     /gene="murB"
                     /locus_tag="PLW16_03455"
     CDS             complement(8344..9441)
                     /gene="murB"
                     /locus_tag="PLW16_03455"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPB69996.1"
                     /translation="MEQASRERLKAACAGRVLFDEPLARHTSMGVGGSADVMVFPADL
                     DDLARCLRCLHEAGLPATPVGNGTNLICRDGGVRGAIVVLKDLQDLRVDRETGERPPV
                     SGTRPYPPGVEILPRRESGDPGDALDSGRRGDGAATQGADGAARKSRRETREPAAGAV
                     SVTAGAGVALQALVALALKEALAGAEFLAGIPGSVGGAVRMNAGAYGRELSDILTAVV
                     WLSRTGELRETPREALTFSYRSTDLPAEAVIAAARFALHPGEAGTIRQAVDETLALRR
                     TKHPLAQRNAGSIFKNPRPTPAGRLIEAAGLKGTRVGQAMVSEMHANFIVNLGGATAA
                     DVIALMELIRVRVRAHAGVDLEPEVRIIGEK"
     gene            complement(9627..11024)
                     /gene="murC"
                     /locus_tag="PLW16_03460"
     CDS             complement(9627..11024)
                     /gene="murC"
                     /locus_tag="PLW16_03460"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPB69997.1"
                     /translation="MRKQKNSKKTDMMRRKVRQIHFVGIGGIGMSGIAEVLLNLGYAV
                     TGSDQNRTDITERLVDLGATIHQGHGAAHVGAADVVVTSTAVRPDNSEVLEAKRRGVP
                     VIPRAEMLAELLKMKFSVAVSGSHGKTSTTSMIATILARGGLDPTMVIGGKLASIGSN
                     ARLGDGEVIVAEADESDGSFLKLSPSLAVITNIDREHLDHYRDLAEIKDAFLQFANIV
                     PFYGATIVCLDDENVRAILPRIKRRVITYGLHREADWRAEKIHFSGRTAKFAVYWRND
                     LQGSVTLNAPGLFNVYNALAAIAAARELEVSFSAVRAGLKTFTGVQRRLEVKGRVNDV
                     TVVDDYGHHPTEIRETLAAARQVWPDRLIVVFQPHRYTRTKALFQEFLTAFPNADRLI
                     VTDIYAASEEPIPGVSAAALADGIRRAGHRDVQYIGDFKAIVEELIRTAQPSDVILTL
                     GAGNVLKIGEAFLNR"
     gene            complement(11190..12332)
                     /gene="murG"
                     /locus_tag="PLW16_03465"
     CDS             complement(11190..12332)
                     /gene="murG"
                     /locus_tag="PLW16_03465"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPB69998.1"
                     /translation="MGGGRKMIVAGGGTGGHLFPGIAIAEEFLGRDPANRVLFVGTER
                     GLEKRVLSQMGYTLETLDVEGVKGRGLAQKFGALAKIPRSLAQSRRILRQFGPDVVVG
                     VGGYASGPAVLMAHWMGLPTAIAEQNAIPGATNRILARFADRIFLTFADERGEFPPAK
                     IRVTGNPVRAAFAATGEAVREKGTAAVEFRHNEGTGHTAPGHPFTLLIFGGSQGAHAI
                     NRTVMEAVSAWRDLADLNIIHQTGPADLEAVRQAYRDAGREADIRSFIMDMAAVAAAA
                     DLIVCRAGATSIAEITAMGKAAILIPFPYAIADHQTRNAEVLARAGAAELIPERDLTA
                     ARLAATVRRYVDDPAALHGMATRSAALGNTHAARDIVDACLALLQT"
     gene            complement(12334..13440)
                     /gene="ftsW"
                     /locus_tag="PLW16_03470"
     CDS             complement(12334..13440)
                     /gene="ftsW"
                     /locus_tag="PLW16_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPB69999.1"
                     /translation="MFKLTETDKRPDVLLLVSTLLLVTVGTVMIYSSSSILAMERYQD
                     AQYFLKKQIFFVLLGLTAMVFLTKVPYQKLRYAAYPGLAAAAVLLGLILIPHVGVKAG
                     GATRWLNLGIITFQVTELVKVSLVVFLAHFLTRKIAQIKRFSRGIAIPLAVTLFIVGL
                     VMLQPDFGSAAILMAILLLMLYLAGTRLVYLGGMAAAFLPVALYLVIHKSYRMARLMT
                     FLDPWKDPRNSGFQIIQSLLSFGSGGTFGVGLGDGMQKLFYLPEPHTDFILSVIAEES
                     GFIGVAIVILLFGVLIIRGFFIAFKAPDLFGTLLAAGLTMTLALEAFINIAGVMGLIP
                     LKGLALPFLSYGGSALLMSLAAVGILLNISAQES"
     gene            complement(13444..14790)
                     /gene="murD"
                     /locus_tag="PLW16_03475"
     CDS             complement(13444..14790)
                     /gene="murD"
                     /locus_tag="PLW16_03475"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPB70000.1"
                     /translation="MELEGKRILIIGMGKTGVAAATFAGRRGALLALTDEKPPAAWTA
                     ALAEVQSTADTPEIRPYAPAALDGVDLVVPSPGVAPSNPLLVEAGARGIPILSELELA
                     ARHLRGPMIAITGTNGKTTVTTLVGDLLKASGKTAFVGGNIGRPLIGFADGPQEEETA
                     VVEVSSFQLHWTETFRPAIGILLNVTCDHVDYHGSFSAYREAKERLFSRQEPGDLAIL
                     NADDPGTPALAARLRAEVEYFRTSGPCRPGISLDGDNLVYAAPDGAIELYARDLIRLH
                     GLHNVENVMAAILACRRAGCPPEAIRQGIAAFSGIAHRIQFAGEKDGIAFYDDSKGTN
                     VGAVARALETFSRPVVLLLGGRDKEGDFAALIPIIRERVKTLILFGEARERIGGLIGE
                     IVATRRAATLKAGLEAAREAAAAGDVVLLSPGCASFDEFTDYRERGDMFQRWVKGL"
     gene            complement(14796..15875)
                     /gene="mraY"
                     /locus_tag="PLW16_03480"
     CDS             complement(14796..15875)
                     /gene="mraY"
                     /locus_tag="PLW16_03480"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPB70001.1"
                     /translation="MLFHLLYPLHIDYSFFNVFRYITFRTIYATVTALVICFIVGPWL
                     IQKLQAMRIGQPIREDGPNSHLSKQGTPTMGGILIIFAVVISTLLWANLTVQYIWLVL
                     LVFLGYGLIGFLDDYRKLTRSNSKGVPGKVRLAGEIAIALFVGVILYVKPDFSSLVTI
                     PFFKTVLPDLGWGYILLSVFIIVGAANAVNLTDGLDGLAIGPAITCFITYLLFAYFAG
                     NIKISGYLQLPYVAGTGELAVFCGAVVGAGIGFLWYNTYPAQVFMGDVGSLSLGGALG
                     TVAVVTKQEILLAIVGGIFVLETFSVIFQVGWFKISHGHRIFRMAPIHHHFELKGWPE
                     PKVIVRFWIISILLALLAMSTLKIR"
     gene            complement(16071..19082)
                     /locus_tag="PLW16_03485"
     CDS             complement(16071..19082)
                     /locus_tag="PLW16_03485"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPB70002.1"
                     /translation="MDLTRLLHTVDLLEIRGNLSGTCESLCYDSRTCGPGSLFVAIPG
                     LKTDGHAHIADALGRGAWVVVHEAPWTPPSGVTAVRVADARRALGRLARNFFGDPSAA
                     LTLVAITGTNGKTTVTYLLEAILKAAGYRVGVLGTVNYRYGDKTFAAPNTTPESCELQ
                     RLLRQMADAGVTHVVAEVSSHAVDLKRVDEVAFDIGVFTNLSQDHLDYHVTMERYYAA
                     KRRFFTEVLPSGGKGRPCPCVVNADDPWGRRLAGEVTLPVLSFGLTERAGVTAADAQL
                     TLAGITARLRCDQGEFPVRSPLIGRFNLSNLLAAAAAALSLGVPTDAIAAGIAALAQV
                     PGRLERVSRGGEPTVFVDYAHTDDALRRVLETLNDHRRGRIVTVFGCGGDRDRAKRPL
                     MGRAAADLSDLTVVTSDNPRSEDPAAIIVQIEAGIAARGIPKLPPEALKPDRQPRMTS
                     TPSPEAPGTASPRGYTVIPDRAEAIAAAIALAAETDICLIAGKGHEDYQILGDRRIFF
                     DDRLIARRALDARKRPLAEARTGGDTERGGPRLDVAAILAATGGELIAGPPEAVLAGV
                     STDTRTLSPGALFVALAGEHFNGHDCLGEALARGAGGLLVSRARRDRLPGAPGGIVII
                     AVDDPLTALGGIAHAWRMRFALPVCAITGSSGKTTTKEMAHAILSQNKRVLATEGNFN
                     NLIGLPLTLLRLDAADEIAVLELGTNRPGEIARLTDIAAPTAGLVTNIGPAHLEGLGS
                     LDAIRREKGDLFGRMDPAGGIAVLNGDDPSRDALKERWPGRTVVFGFDPAHDVFADAP
                     APAAGGGTRFTLHAGGAAREVTLAAPGRHNVANALAAAALAWAAGTDPDTIAAGLARF
                     RPVAGRMQIFTLASGAHLIDDAYNANPASVAAALQTLAGLRESGAGVAVLGDMLELGE
                     QSENLHAEIGARLVETGVSQVHLTGAFARTTADAAVAAGLARERIVFFTSPEEVVADL
                     KDRLHAGDWILVKGSRRMRLDAVVQAIRETL"
     gene            complement(19093..21108)
                     /locus_tag="PLW16_03490"
     CDS             complement(19093..21108)
                     /locus_tag="PLW16_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB70003.1"
                     /translation="MNREAKKWLRFRLASLMAVFLVLFIALVSRAFQLQILSGTTLKS
                     LAERQHVKTLQVPPERGIIFDRNGEKLAVSVMADSVCADPAKITRAGDTAAKLAAILD
                     QDAGQIRRKLSRKGNFCWIARRIPPDRAARVVALNIDGVFLIQEPKRFYPSGELAGHL
                     LGFVGTDASGLEGIEMLYDRHLMGAPRKLTRARDAKGKPLYARVETVAVTPEESRNLI
                     LTIDSRIQYLAEQRLKEAVLDKGAKGGYAIVMDPRTGEILALANQMTFDPNNFGKFGH
                     QVWKNKAITDCYDPGSTFKPFLVAAALEERVVRENDRFYCENGAYAVADRVFHEAQRK
                     RHGTLTVHDILKYSSNIGSIKISERLGKEKFYEYIQKFGFGAKTGIDLPGEVPGLLRP
                     VQNWTRVDASTVAFGQGVSVTAIQLITALSAVANGGVLMKPYIVRAIVDRKGQIIQSN
                     SPQPVRRVVSPATARRLGAMLTDVVGEEDGTGKRARIENVSVAGKTGTSQKYDTARHV
                     YSSERVRTSFMGFFPAEDPQVAILITLDEPQRDKWGGVAAAPVFKGIGEQILACFKSH
                     LRESPAPAPSPQDSAPPAHRLTLVSASRTLPIPGTAVSGAEESTIDEAVMPDFRGLTI
                     RDALKRARERNIRLRVLGSGWALNQAPSPGTPLVGERLCTVSFHTGD"
     gene            complement(21105..21446)
                     /gene="ftsL"
                     /locus_tag="PLW16_03495"
     CDS             complement(21105..21446)
                     /gene="ftsL"
                     /locus_tag="PLW16_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPB70004.1"
                     /translation="MTAPDAPKKDRLPEEKPASRWPPHSVLIVGAFVFMAVALAYVWC
                     HIHMTTLEYRVAEALNQKETLIEEQRRLRLELATLKAPRRIEAIARDRLQMTAPTLEQ
                     VVMLKTGEETP"
     gene            complement(21443..22384)
                     /gene="rsmH"
                     /locus_tag="PLW16_03500"
     CDS             complement(21443..22384)
                     /gene="rsmH"
                     /locus_tag="PLW16_03500"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPB70005.1"
                     /translation="MECFHAPVMLGEVVASLNCRRGGIYIDGTLGGGGHAAAILARTA
                     PDGFLIGIDQDEEALTAARERLAPFRDRTLLIHGNFARLEALLSDRHPGPADGVLLDL
                     GVSSHQLDTASRGFSFSRPAPLDMRMDRSRGITAQELLSSASASELERIIREYGEEIM
                     AGRIVRAIVAGRRQAPIATTAELARIVCAAVPPPLRHRRIHPATRTFQALRIAVNDEL
                     TRLRQALPQCRDRLRPGGRLSVITFHSLEDRIVKETFRTWEKGCICPPGLPVCACGLT
                     PTARSIHRKPIAPDSAEIDRNPRARSARLRTIERTAP"
     gene            complement(22401..22847)
                     /gene="mraZ"
                     /locus_tag="PLW16_03505"
     CDS             complement(22401..22847)
                     /gene="mraZ"
                     /locus_tag="PLW16_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPB70006.1"
                     /translation="MSVFRGQYHHTIDDKGRIIFPSRLREVLAEKYDNRLVITNWDGY
                     LMVFPYEEWRIIEEKVSFQSLLKKEVRAFQRFFMSGAVDCAIDGQGRVLIPPNLREYA
                     HLEKDVVLAGMIRIIEIWSKERFEAEMAKTGGDPDTLSECMAELGI"
     gene            complement(22932..23954)
                     /gene="mltG"
                     /locus_tag="PLW16_03510"
     CDS             complement(22932..23954)
                     /gene="mltG"
                     /locus_tag="PLW16_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPB70007.1"
                     /translation="MSKRKSFKHRVWRTAGTIILLLALFVPFYVYHTNTAVAPETRTV
                     TVDIPQGTGFFGATDILDRAGLIKNRPLYYVLVIGKQAHRQIRAGEYEFTTAMTPRQI
                     VKKLVKGEIKHHRVTIPEDLTLREIAERLAEHKLADPEQFIALAHDRKFLASLDIDAA
                     SAEGYLFPDTYLLDRTMSQREIMRLMVREFRKRVTPDMIVRAEALGMTMGEWVTLASL
                     IGKESGCRSEKPLISAVFHNRLKKGMKLQSDPTAVYDLAHFNGTITRRHLRRRVPHNT
                     YWIAGLPPGPIANPGLDSLKAALEPAPVDYLYFVSNNNGSHYFSSTLLAHRQAIVKYQ
                     TDRKKN"
     gene            complement(23947..24459)
                     /gene="ruvX"
                     /locus_tag="PLW16_03515"
     CDS             complement(23947..24459)
                     /gene="ruvX"
                     /locus_tag="PLW16_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPB70008.1"
                     /translation="MEPTGAKRAVGAIEVGRVYATEDHMRIMGMDYGEKRIGIALSDE
                     LGLTAQGLPTIVRKNRRQVLEAIGDLIARYGVERIVLGYPIRLDGSEAVQCAKVNRFA
                     ATLRASFDLPVIRQDETLTTREAEDLMREAGLRRERRRRSVDRVAASLILQGYLNACR
                     SRTESGTIHE"
     gene            24684..26603
                     /gene="selB"
                     /locus_tag="PLW16_03520"
     CDS             24684..26603
                     /gene="selB"
                     /locus_tag="PLW16_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPB70009.1"
                     /translation="MKHLIVGTAGHVDHGKTALVRALTGVDTDRLKEEKNRGITIELG
                     FAPLTLPSGLAVDIVDVPGHERFVRQMVAGAAGIDLVVLVIAADEGVMPQTREHLQIC
                     ALLGIRRGLVALTKTDMVDPDWRTLVAEDVREFLQGTFLEGAPVCPVSALAGEGLGDL
                     LGALDAVAAAVEEEEDCGLFRLPVDRVFTMKGFGTVVTGSLISGAIAAGEAVDIFPRG
                     VTAKIRGMQIHNEGVTEAAAGQRVAINLQGVEKAMLGRGDVLARPATLLPSRRLDVRA
                     SSLASAGRALKNRALVRFHAGTSELMARIILRDRDELKPGEAAAAQLFLEAPACCVAG
                     DRFVLRSYSPVTTVGGGVVLDPLPKKHRRRDPEVASALTVLAEGDGAARTAVILERSG
                     ARGAAPGELCVRTGMPVRRLQKILDGLFAAHRAVLVDRDEMRAVAAGVYQDLQNRIVA
                     DLTDYHARHALKEGLSREELRTALGRAVTPKLFQMVLRDLEKAGRIVADREHVRLPGH
                     QVQLGGELAALRQEMGDLYRDAGLAPPTLREIRERFADRVRPAESVLKVMLQEGELVK
                     VSEDLYYHGDNLAALRERYRVLLIRDGKATPVEFKELTGLTRKFIIPLMEYFDLTKLT
                     IRSGDQRLLREKEGK"
     gene            26600..27385
                     /gene="fdhD"
                     /locus_tag="PLW16_03525"
     CDS             26600..27385
                     /gene="fdhD"
                     /locus_tag="PLW16_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPB70010.1"
                     /translation="MTALAVSAFLTFPVQVQDGRDRIETSAKIVREIPLEVFLDGRRM
                     GTIACSGLHPEELALGFLRAEGLLEDRRELTRIETDAGGRVVRVHRRRETAAPAVAPE
                     GQTIGSSGARGLGLAASPAPLHDIDLAVTPAQVLALMDVLLNGSAIHNLTHGTHCSAL
                     ATPEGIAVLREDIGRHNTLDMLGGYMFLNDIPGRGRILLTTGRVSAEIVQKVWRLGVP
                     ILISHSAPTSRALELLAEAGITLIGFVRGGRMNLYTEGKRVIR"
     gene            27385..28341
                     /locus_tag="PLW16_03530"
     CDS             27385..28341
                     /locus_tag="PLW16_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB70011.1"
                     /translation="MTKSIYIKDIKAGDKVSTAFLVAEKNMAYSQKGAPYLNLKLKDR
                     TGEVDAKVWDNAPQWDPVFGKGDIVHCQARAVSYKNALQLSILEIRRLEAGDVDPADF
                     FPCARRDPEAMFAELEGLVASVTAPHLKALLAAFFADAEVVGAFKRAPAAKGFHHVYI
                     GGLLEHTLSVVRLLDVASRHYDGLDRDLLLVGGILHDIGKIREYRYEGLIDYSDEGRL
                     IGHIVIGVEMLDEKIATLAAFPPETAMALRHILLSHHGEMEFGSPKRPKTLEAMIVHQ
                     LDDLDAKVNAIQGFVAGTPDDASAWTPFHRLFGRFLYKGKPG"
     gene            complement(28598..29563)
                     /locus_tag="PLW16_03535"
     CDS             complement(28598..29563)
                     /locus_tag="PLW16_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPB70012.1"
                     /translation="MSIKEISLSELDPRGFIAEQVRAINAAVGDGAAVNALSGGVDSS
                     VVTLLGHRALGDRLKTYFIDNGIMREDEPRQIQEAFAAMKVPVEIVDARAEFFEALKG
                     LTDPEEKREAITQTFYKAVFGRLVKESGARHLLQGSIFTDVEETVAGVKRQHNILSQL
                     GIDTEAAFGYKVLEPLIRLRKPGVRLVGRELGLPEDLFNRPPFPGPALAARVIGEVTP
                     ERVSTVRQATRIVEEALCKSGAFQYLAILHADRVTGMRHGARDYGCQIELRCWNSTDA
                     RTGSPTRLPYEVLEGLADRITTEVPGVVSVTYNITKKPPSTIEAI"
     gene            complement(29692..30666)
                     /locus_tag="PLW16_03540"
     CDS             complement(29692..30666)
                     /locus_tag="PLW16_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB70013.1"
                     /translation="MTLQQEKIRAARLSIASNSLLILFKLIVGLSIGAVSILSEAIHS
                     AVDLVASVIALFSVRTSSVPADTRHPFGHGKVENISGVVEALLIFLAAGWIIYEAVER
                     ILHPRPLGYIAWGIGVMLLSAAANYAVSARLFRIGRAADSIALTADAWHLRTDVYTSA
                     GVMAGLAAIWLGQRFAPDLDLHWLDPAVAIAVAFLIIRAAWHLTLQSARDLLDVRLPP
                     EEEAWIRQLIIDHRPIIHGFHRLRTRKAGNFRFVEFHMKVDPDMSVQESHRITEELAR
                     AIEWHFPSASVTIHTEPCDGDCVGNCLTGCLLTEEKRRDIMAGKKIAG"
     gene            complement(30685..31368)
                     /locus_tag="PLW16_03545"
     CDS             complement(30685..31368)
                     /locus_tag="PLW16_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70014.1"
                     /translation="MPRILPVPLGTANTYIVSEGPASILIDAGFPRQGRKFFTTLSKQ
                     GMDPARISLIVITHVHYDHVGNLREIRDACRCPVAVPAGEADWLRRGAVVFPAGVHPV
                     GRALIGAGRLLSRVLPGFVRFDPVEADVLVDGELSLDPFGMAGRIVMTPGHTAGSLSV
                     LLDDGNAFVGDLAFNVLPCGGGPIMPPFADDPAALKQSWAALGRLGARTIWPGHGRPF
                     SAHRLRSHA"
     gene            complement(31521..32660)
                     /locus_tag="PLW16_03550"
     CDS             complement(31521..32660)
                     /locus_tag="PLW16_03550"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70015.1"
                     /translation="MHTLTPEALKTLIQKQTGPCLSLFMPPSAVGTKPQQSQIRFRNL
                     LRKAEETLAAHGLKPPDVKTFMEPLSGITGQALFRKNRRDGLALFLSRDVMNLYALPL
                     DVAELVVVGERFHVKPLLPLLGDENRFFLLALSQKAVRLFEGSRFGLRERPLEHCPRS
                     LEEALQYDEPERQVRFRSRAGAETATRSGHGAGADQVKDDILKFFRLIDRGLRDLLKE
                     EKAPLVLAGVEYLFPIYRDANTYPYLMDEGIQGNPEEAEEEELRRQALQILKPYYEKA
                     EHDAIAQFRQSSGTGLTATDIREIVPAAVHGRVGILLVASGRQQWGVFTPESGAVTLH
                     AAMKPGDEDLLDVAAIQAFINGGEVHILPPGKIPGQEVLAAVYRY"
     gene            32992..33555
                     /gene="efp"
                     /locus_tag="PLW16_03555"
     CDS             32992..33555
                     /gene="efp"
                     /locus_tag="PLW16_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB70016.1"
                     /translation="MYTASDLRKGLRIKIDNDPYVVTEFNFVKPGKGQALYRCRLRNM
                     INGSQFERTFRSVDTFESADLQEKKMQYLYQEEDKYCFMDNESYEQVFLTREQVGDAV
                     NYLIDNIEAQVLFFEDRALGLSVPNFVDLVVTQADPWAKGDSVTGNQKPVTLQTGYQI
                     LVPTFIEEGEKIRVDTRTGEYLTRVKG"
     gene            33542..34486
                     /gene="epmA"
                     /locus_tag="PLW16_03560"
     CDS             33542..34486
                     /gene="epmA"
                     /locus_tag="PLW16_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HPB70017.1"
                     /translation="MSRDEPDAGGEWRLTRRRAALEIRARMIQALRRFFVTAGYLEVE
                     TPVRIPAPAPETHIDAPPAGDWFLQASPELCMKRLLAAGYGKIFQIAKCYRAGERGDR
                     HLPEFTMLEWYRPASDYRDLMTECEVLLPFVLQALGRDAALVYRGERIDLTAPWERLS
                     VAEAFRRFTPLTPEDALRKACFDERMALDIEPRLGRERPTFLYDYPAACAALARRKGA
                     DPALAERFELYIAGLELANAFSELTDVAEQRRRFEMARTERERLGKVGYPSAEPFLKA
                     LGAMPSAAGIALGVDRLAMLLADADRIDDVVAFVPEDL"
     gene            34753..35505
                     /gene="tolQ"
                     /locus_tag="PLW16_03565"
     CDS             34753..35505
                     /gene="tolQ"
                     /locus_tag="PLW16_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HPB70018.1"
                     /translation="MNEISTVAAGMGGQFHGSLLNMILDAGLMVQLVLILLFVFSIVS
                     WAIIFLKYRTIRKVKQENGLFLDAYMQSSKLAEIFPEAKKYATSTLAAVFRAGYGELV
                     KLTKARRELAGGREEDGGLATLELRGIDNVERALNRAASEESSKLEMALGFLATTGSA
                     SPFIGLFGTVWGIMDTFKGIGLRGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNY
                     YLQRIKAMTAEMDNFSGEFLNIIERYQLKKGQ"
     gene            35547..35930
                     /gene="tolR"
                     /locus_tag="PLW16_03570"
     CDS             35547..35930
                     /gene="tolR"
                     /locus_tag="PLW16_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HPB70019.1"
                     /translation="MADINVTPLVDVMLVLLVIFMVTAPMMQMGIDVNLPRVQSKSID
                     VAEEKIVLSVNAQRELFLNRIRVPLGELRPKLEAIFAARIEREVFLRADRTVPYGFVV
                     EVMSEVRRAGIDKLGMITEAPEERG"
     gene            35984..36676
                     /locus_tag="PLW16_03575"
     CDS             35984..36676
                     /locus_tag="PLW16_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPB70020.1"
                     /translation="MLWLSLILHLALVSVILSLPPLPQAKLTLGPVYSVELVSAPPGL
                     PAPSSDLSPDILNPEAGRPAAVLKNMKASTLPERRTEALRKRPEAAEAAVEAIRRKMA
                     AQPPAVPAPRVTAETTAPTVPAEGRASGAERQAQARRYAALVSQLVWSQWAFPRDSLL
                     PVGSEAVVHVTVLANGTVARMGFEKRSGNRYFDESVLKAIRKASPFPALPAEIGGGRL
                     EVGIRFHPSRGQ"
     gene            36695..38026
                     /gene="tolB"
                     /locus_tag="PLW16_03580"
     CDS             36695..38026
                     /gene="tolB"
                     /locus_tag="PLW16_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPB70021.1"
                     /translation="MIFRGFCVLLLGLGIFLGGATTPGWAVEGAERVYIDIDSPHFQK
                     FPLAVTPFYALGPSPEGAELSRWFSASLTEMLAITNFFQLIPPTAFLEDPVHADSTFQ
                     GTHFPDWRAIGAEYLVKGGYRVRGQELTAEFRLFDVVRGEVVRDKRYAGPLAERKAMV
                     QKFAGEILFALTGDGGVFNTKIAFVLKPGREGEIHTVNFDGSDLMRFTRWKTLTLSPR
                     WSPGGRYLTYTSYRDGNPDLYVQGYPGGGTKKIAAFPGLNLAGPWSADGQRMLLTLSR
                     DGNHEIYVMSLEDGRLERLTHSAAIDVSPTLSPDGRSIAFVSNRSGSPQIYVMNREGG
                     EVRRLTYEGGYNTSPSWSPKGDRIAFEARVGGSFQIHTIRVDGGDQTPLTTDGGDHES
                     PSWSPDGRYIAFSTRGAGVSQICVMNANGTGKRVLVEAPGCLAPAWSPRLP"
     gene            38183..38821
                     /gene="pal"
                     /locus_tag="PLW16_03585"
     CDS             38183..38821
                     /gene="pal"
                     /locus_tag="PLW16_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPB70022.1"
                     /translation="MIHNRTRMKVLVCLFCFAMMVVLGCATTSSQKEVLVEGEDAVAA
                     EQTGAAAEAVKPGEPEPIIDETLAEPDAAAPQMAADEVPPVPVAKPASTVSAASEAPA
                     WENIQFDYDKFSLNPEARVLLDKLGEWLLRNKGYAVVIEGHCDERGTTEYNLALGERR
                     AMEAKAYLTGLGVAAARIKTISYGKEMPLDPGHDEEAWAKNRRDQFLVELPR"
     gene            38841..39716
                     /gene="ybgF"
                     /locus_tag="PLW16_03590"
     CDS             38841..39716
                     /gene="ybgF"
                     /locus_tag="PLW16_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPB70023.1"
                     /translation="MRKPLSLVSLAVFLILVGCASQQDLKRVRGELDQRITAVDQRAA
                     RLEQQDLERQEAMAALMPEVGKNREDIAAVRKAQASQGADMTELRDQVQQLRGTVEGL
                     QKEAAVLSGRAGRKDEEVKDLRERLDAAAFKVNFIENYLGIGKKEVRSENGDKSGKSV
                     RESLKGKTDKDSAYAMAYETFKEGRYEKAREEFQNFLKAHPATEYSDNAQFWIGECYY
                     LERKYEKAILEYEKVVKGYPEGDRAPHALLKQGLCFQSLGDHSSARLILQQVIRAYPN
                     TSSARIARAKLMEIK"
     gene            complement(39831..40670)
                     /locus_tag="PLW16_03595"
     CDS             complement(39831..40670)
                     /locus_tag="PLW16_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPB70024.1"
                     /translation="MHTTVPQSPAPLNKTALLLLVVLMLGIAGCRPPTGALRPTPVGT
                     IPEHTLAALASADRFDGTFQALTRIELSGDRGAYPLKAAILMKWPDALRIEHLPLLGP
                     PDFFMTLAGSRLQISVPGEKAYWTGAPNRENLSSFLPIGLSGGELIALLRGSCPPGEG
                     ITSVRTVPEADTDAGRLDLFDPYGKRLTLWTDPASRHVQRCTRFAEDGKELYTVRFED
                     YRLLSGRPWPMRIRFTAPGPPATKMSVRYTDPVLSPDTVPERFDLPIPPGTLPRRLPE
                     GSP"
     gene            complement(40680..42386)
                     /locus_tag="PLW16_03600"
     CDS             complement(40680..42386)
                     /locus_tag="PLW16_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB70025.1"
                     /translation="MNFDLLRKGLLILLLLGAFSGCAPLTPPAPPVAEPSEDKASREA
                     AYHYSVGIFLALDGKTDEAIRELETAMGYDPYAPAITLELAPLYLEKGNPAKAIALCD
                     KTLLRHPDDIDVHLLSGGLYLNAQDTKNALRRYRRVIELDPVNESAHLYLGTIYADSH
                     RYDKAAEHFRKLLEINPEHPMGNYYLARVLKDMGLLTEAEAGFRKTLALKPLWEAVLL
                     DLGSLYERQRRTDLAIQLYRDFLATYPNRITVRLRLGQLYLREQNFGEAEQAFATILS
                     QDRSHQEARLALGIVYLEKGRPDQAIEQLLALLLERPEDAKLTYLLASAYEEKADFPK
                     ALEEYRRVPVSSDYFGNARIRIGMILNRQGQPDAAVASIREALTLNVALPSLYLYLSQ
                     LHEEQKNLQAAEDCLAEGLRFSPESTDLHYRRGVLYEKTNRFEAAIQAMEQVLKIDPN
                     HAEALNFIGYSYADRGIHLKEAERMIRRALKISPGSGFIIDSLGWLYFRQNKLDLAVK
                     YLKEASGLLPGDATIAEHLGDAYAKAGRSREASEAYARALELTPESASLKKKLQDLQK
                     RK"
     gene            complement(42401..43318)
                     /locus_tag="PLW16_03605"
     CDS             complement(42401..43318)
                     /locus_tag="PLW16_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPB70026.1"
                     /translation="MGKKKKKKRTGWVVVLFLALLAGALFIPLTRHWEAMRLPTPDGT
                     PTGRGKSSPVDLLPPDAERRPPDARPRSPRAPAWKVAILIDDIGHDLRPLRELLRLGI
                     PLNFAVLPYRRHSREAAEMIHDAGQEVLLHLPMEPRGYPGEDPGEGALLVALNDAEIA
                     RRVERALDAVPHIRGVNNHMGSAFMAEEGKVSAALAVIGKRNLYFVDSRTTANSRVEA
                     LAVLAGVPFAARDIFLDNGRDAGATLDILKQAVRKGPPGARNVLVMIGHPYPETLAAL
                     REALTFLKVEGTETVHLSSLLLEGEPLRR"
     gene            complement(43353..44645)
                     /locus_tag="PLW16_03610"
     CDS             complement(43353..44645)
                     /locus_tag="PLW16_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPB70027.1"
                     /translation="MKTPMQKRRLILAGVSALFLILGPYGDHQVSALDRGTYKHLKTF
                     NEVLDMVEKNYVEDIEAKTLIQGAIHGMMKSLDPHSAYMTPEMYKELEVETRGSFGGL
                     GIEITLLKDILTVVAPIEDTPAHQAGIKPGDQIVKIDGQSTKDITILEAVKKLRGPKD
                     TKVTLTIVREGLTKPKDVVVTRAIINVKSVKTKALDPDIAYIRVLQFQERTGEDLKKA
                     LREVTDGGKLLRGLILDMRNNPGGLLNQAVEVSDIFIKSGVIVSTRGRAKSMETKAMA
                     RDDGDEPACPMIILVNEGTASAAEIVSGALQDNGRALILGTQTFGKGSVQTVIPLEDG
                     SAVKLTTAKYYTPKGRSIQAEGIAPDVIVKFIRPAEENDQADEPLRERDLRGHIRPQK
                     ENGPPAEELRKETDLDNQLKAAIDILKSWDVFRKNMRS"
     gene            45032..46651
                     /locus_tag="PLW16_03615"
     CDS             45032..46651
                     /locus_tag="PLW16_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70028.1"
                     /translation="MTEKYDVVVIGAGVAGLGTAAILARDFKQKVLVLERNPFIGGRL
                     ASFVGKGDKVCIDGMELDANGFKRVLGMVGAWVPKCTPDLETCFKEKIFDGCTLDTGH
                     GLFWGNKGKIRMLMNYLEKPVDMPCNVGFAFVDYKKGNKAYQVGKGEPYAWMSKEGFR
                     ATMMALRDMALMSLADIAQTAHMSFEQWLQARNVPKEAYDYIKVLAASQTGQADLNLT
                     PAPDVLGHMATAGPIRMNLVEGSCATVANPGTMAFALLMEQALTAHGGKVLRSAPVVE
                     VIIENGVVKGVTFQYEKGIETAYADRVICTVPSKQMLNVIHPRYFPQEIVDRVQTKFW
                     GAGMITGYGNMKSDIWADKGIEERSFMYMPDVIGAEEGYSGCIDMVLWNLASVARGSN
                     SNPSLTSETGAAPEGKHGWIFSTAMTHEEMRTPRKVARVVEWNEDWWKKTFGRAKWEA
                     EMEFLLWMCTDHAFGWIQPIGTDRIDVKSPYVDGLYFAGDQYGERLWGCGVDAAALSA
                     TLCVDSMMGSNAEEKVFPFYHRAVPPWKKAW"
     gene            complement(46849..47871)
                     /locus_tag="PLW16_03620"
     CDS             complement(46849..47871)
                     /locus_tag="PLW16_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB70029.1"
                     /translation="MWDALKCDLCGDCLVKCRYVDYDKDRAASEMKLLMEGKAADILD
                     HCITCNACFQYCPTGADPANLICRMQEKMGSPITVGFKPVINSVVKTFEQGSRDIQVI
                     EGDPDKPALSFDSFTFAQFPAGTLESRMFKGMTVVRGRKYASLVGMAHMGGESLAEKY
                     GRRVIDSLAELGKEIVYMHNEGYILAHIKARELGIEVPYKYLHLFEYLLDYFKENQRD
                     IKKLKKMVAYQPNCAVRWFPEQDIWLNEIFELIGVERVSRTYEGVNALCCGGPALFVN
                     NELSMGIQKNNIRDAMDNHAEAMITICPMCDTVLRDQTAKAGLPQIFITDLCRMALGE
                     VSWPVR"
     gene            complement(47999..49582)
                     /locus_tag="PLW16_03625"
     CDS             complement(47999..49582)
                     /locus_tag="PLW16_03625"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPB70030.1"
                     /translation="MSFSRINTSSATLTKNRTEESITPTSGMCVTCIDGCIGMCEIGK
                     SAYRGHEVIYPQPFGVITTAAEKKFPVDYSHFNIMGTASGAFGIEADSDKAIFPAVNL
                     EVNIGHDKGIKFKLPWLISGIGSTNIAKNNWEGLAIGSALAGTGLTIGENVVGMDPET
                     KFKGGKIVDTVDLKRRVKLYKDNQRDGYGAIIVQANVEDSRLKVQQYAIDKLGVQCVE
                     LKWGQGAKDIGGEVKIKDLKKAQMLYDRGYIVLPNPHDPTVIAAFQKGAFKEFERHSR
                     VGMVTEEGFAETIEGLREAGAKYIFLKTGAYRPVDLARAIAFASKYKLDLLTVDAAGG
                     GTGMSPWRMMNEWGVPPVELHTLVYQYAKQLADRGKYLPAISVNGGFTFEDQIFKALA
                     MGAPYVKMVGMARAPIAAAMVGKTIGQTIEAQQVPVYIERFGNTKDEIFVTASTLRKE
                     LGDKEFDKLPTGAIGLYTYYERLAQGLRQLMAGSRKFSLEYMSRNDIASLTPEAARVS
                     GIQYIMDVDEDEASRILNF"
     gene            complement(49608..50282)
                     /locus_tag="PLW16_03630"
     CDS             complement(49608..50282)
                     /locus_tag="PLW16_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB70031.1"
                     /translation="MINLTLNGLSISVEKGTTILEAARFYGFPIPTLCHKEGLTPYGA
                     CRLCVVEIGKGPGAKLVSSCTYPVEEGMMVRTASERVLRARRMIIELLLASCPQSKTI
                     QDIASRHNVRQQRFRQEHEDCILCGLCVRMCKEQMMAGAIGFRGRGQNRSIGTPFDIR
                     SDVCRLCGGCIEVCPACQARCTYNEPEKAVCGGCANLSPPCIEKEQFHDMMCYMSPCV
                     ACEAKK"
     gene            complement(50275..53529)
                     /locus_tag="PLW16_03635"
     CDS             complement(50275..53529)
                     /locus_tag="PLW16_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB70032.1"
                     /translation="MKTFQTVAAFNAYRASFLTDKNEEKKPCIVICGGTGGQASGSND
                     LIRIIKRRILEQNLHDKLSLRVTGCLGFCEMDPFIIVEPGYNLYPKLKMENVPKIIDA
                     AVKGEVVEELLYREPGSTVKRYSEQEIPFFQGQTRIVLGQNQHVDPIRIGDYLKMGGY
                     AALEKALEKQDRAWLVDEILKSKLRGRGGAGFMTGRKWEMARNMKNGGGPKYIICNAD
                     EGDPGAYMDRSMLEGNPHAIIEGMIIAALAIGASDGIIYVRTEYPLAIKHATIAIREA
                     RRLGLLGKNILGRGVDFEIEVFHGAGAFVCGEETALIKSIEGKMGEPRQRPPYPIEKG
                     LFGHPTCINNVETLANVPYIINHGAEAYTKIGVENNAGTKIFSLVGKIKNTGLVEVPL
                     GTPINKVVYDIGGGPVSAARIKAIQTGGPSGGCIPASMFDLPIDYDSLAKAGSIMGSG
                     GMIVMDENTCMVDVAKYFMSFLKDESCGKCFTCRKGTQRMHEILEDITEGRGTPEHLV
                     LLEELALTVKDTTMCGLGQTAANPVMSTLRYFREEYERHIEDKKCGAFVCKQLTGAPC
                     QAACPVDTEPWRYVAMVEKGDYEEAYRVIRTANPFPAVSARVCDRKCENRCSLGVSGG
                     EAIAIRAIKRFVTDRVNPSVYKPQRARKKKKEVAVIGAGPAGLTAAHGLSLIGYGVTI
                     YEAENQPGGMLTCSLPAYRLPRDIVEKEIKTLLNPNIKIEYGRALGKDMTLQDLLNDR
                     FDAVFLSIGAHESWRLDLENEDVEGIFPSIQFLKAFNIRGEELARGHVGIIGGGNSAV
                     DAARVALRQKDVTSVSLFYRRTREEMPAYEEEIDAALEEGVKVETLISPVKIHVRQED
                     IEAALKEGVELETLVTPIKIFSKEGRIIGIECIKNRLGEVDATGRRRPVPIPGSEFRV
                     ELDTLIVAIGEKPKSEFLASMGLDVNRGGRLKVDRKTLETSLKGVFAGGDLVTGPNTV
                     IDAIAAGKKAAKSIDRFLQKGKIEKAAAPKLPSTYIEPAVIDETEAQTARRAEPAVLA
                     PHERVKNFREVTLVLPEDKARAECRRCLRCDLAFTRDKSASQAASHTIEGAKHD"
     gene            complement(53548..54399)
                     /locus_tag="PLW16_03640"
     CDS             complement(53548..54399)
                     /locus_tag="PLW16_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB70033.1"
                     /translation="MDTENILEIIKRSDKNRGAIIAILEEIQATYTYLPEAALRLVAE
                     ETGHSLTDIYGVATFYKAFSLKPRGKHCISVCLGTACHVRGAQAIVDEFKYELKIAPG
                     GTTPDNEISLETVNCLGACALGPTVVADGHYFPHVTKGEVREIIARTKEEMEKINTET
                     DDRIFRLPLSCPLCKASLMDPDHPLDGHPSVRIDVSANGRKNPLRLSSLYGSGAVAME
                     DPVPLNQVSPLFCPHCDEELPSFSICPECGSPMAALLVRAGCAWEICTRRGCRGHLLN
                     LNQINLS"
BASE COUNT         9239 a        17045 c        18274 g        10171 t
ORIGIN      
        1 acgtgaacga tctggccgac gcccacggcc tcgcgctgga acgtctggcc ctcccgtctc
       61 cgccggggcc gacgggcgcg gtggtcgggg acacgccttc gtccccgggg cagggcgggc
      121 gggccgtccc cgggacggcg ggagaatcgg ggggtgccac gcccgcgtca cacaacgagg
      181 acgcggtgac gggcattcgg gctggagaag ggagggttcc ggaggaagcc gaaggcgggg
      241 ccggtgggcg ggcactggtc atgaattgcg gctacggcca cgggttttcc gtcagtgagg
      301 tcgtcgccgc ggcgaagcgg gtgacgggcg tcgacttccc cgtcgtctac gaaggccgtc
      361 gcgccggcga tcccccggcg ctcatcgcgg acagcacccg gctccgccgc ctgaccggct
      421 ggaccccccg gcacgacgac ctcgatttca tcatccgcac cgcctgggac tgggagcgca
      481 aacgataagg ggaggccttt gcacaacggc ctattttttt atttgatgct ccaggggtcg
      541 caggcggttg cgggtttgga agcggtttcg aacgggatgt ggagttcgta cgtcccggcg
      601 gagagggtcg ccttgatggg gaagggcgcc ttctcgtaca ccttcatcat cgccttgttc
      661 gcgacgagca cgtccgccgt gaagccctcg atccccttcg actgcgcgat ctcgatcagc
      721 atcttgaaga ggtaggtcgc gatcccgtag ttctggtagg cctcgtccac gatgaacgcc
      781 gtgtccgcgt acgtgcgcag gtcgtcgcgc cgcacgtacc gcgcctccgc gatgatccgc
      841 tcccccgccg cctccgccag caccgccacg atcgacatga cccgccggta gtccacgttg
      901 acgtactcct gcatctttcc gtgaggcatc gtcttgatcg ggctgaagta gcgatagtac
      961 acggcctggt cggagaaccg gtaaaagagc cgccgcatct cgtcctcgtc ggagggcttg
     1021 atcgcgcgga acctcacctt cagcccgttc ttgaagatgt gggagcgggc gatcttatcc
     1081 gggtaaaggt gcccggactc cggcaggtag atctggtcgg cgtagaggat gcgcgcggcc
     1141 ttcgcctggc gcacgagctc cgcccggtcg tccgggtggg cgatgtcgat cagggcctgc
     1201 gcgcgctcgc gcacggtgcg ccccgcgagg tacgccacgc cgtactcggt cacgatcatg
     1261 tcgagcgact cgcggttcga gaactggaag gggtagtccg ccgtggacag gacgatattt
     1321 gactccccgc cgcggttgcg gctgggcagc gcgaagagcg tgcggccgtt cttggagatg
     1381 gtcgcgccca tgaagagctc ctggaccgcg cccgggccgg cggtgacgtt tcccttgccg
     1441 atgtgcagcg cgatgtcgcc cgtcaggtcc gccttgcgcg cgggcaggat ggcgatgaag
     1501 ttgtcgttct ggccgatgtt cttggggtcc gtgatgacgt cgagcggctg gaactcgatg
     1561 agcgggttgc ggtcgagcca cttcatgagc gcccgtgtcc ccatcgcgta ggagccgacg
     1621 gacttgcccc ggaagagggt cttgaagcgg ttcgtcaccg ccccgctctt gacgaggcac
     1681 atgagggcgt cggtgatgaa cgggctgtgg atgcccaggt tcttcttgcg ggtgagcgcc
     1741 tgtgccgtgc cctggtagat ggggcccaag gcgaaggaga tgcagctccc gtcggggatg
     1801 acggaggcga tgttcttgcc gatcttggca aagacgtcct cgagcggcca ggggtctatg
     1861 taaatcatgg gctccgtgga gtggacgagg tagtcgaaat cctcgatccg caccagcgtg
     1921 tcgcccagga cgaaggggac gtcctcgctg atttcgccca cgcggagagt ggcgcgctcc
     1981 atcgcctggc gcgccacgtc cataccgacg ccgaggcagg catagccgtt cgcatccggg
     2041 ggcgagatct ggatgaaggc cgcgtccacg cggatcgccc cggatttgaa gagccacggg
     2101 acgcgcgaga aacggctggg gatcaggtcc acgcgccctt ccgtgatcgc gtcggccgcg
     2161 atccagccgg agaagaaggt cttcaggcgg tacttgcggg tatagcgcgc gtcgatggag
     2221 accgtgtcac ccaagctgac gagctggatc atctccagat cctggaggtt agccgcatcc
     2281 gaggccatca gatggcgcac cagcgtgcgc ggctccgcca tccccgtgct caggaagatg
     2341 ctcatccccg gctggatctt ctccaggact tcgcccggcg ttaccacctt gtcccgccat
     2401 gcctctttct gagttccttc cgtcatcttc tcaccttctt ccggttcccc ctttccgcgg
     2461 cctccttata atggaattcc ccccgtttgt ccatatcggg catgatggag gtaaaatccc
     2521 cagaaatttc atcctccggg accttcacac aacggcctat tttgtccgac aatctttctt
     2581 gccatttcga ccgaagggag aaatcttcac ggaggccctt tcacgtgtca tttcagaaga
     2641 gcatagcgca ccaccgcatg tgtcatttcg atcatccttc ttgtcatttc aatcattctt
     2701 ctcgtcattt taaccattct tattgtcatt tcgaggagcg caagcgacga gaaatcttga
     2761 agatttctca catacgttcg aaatgacaaa gagaagggac aagatttctc ccttcggtcg
     2821 aaatgacagg aaggatgatc gaaatgacaa gaagggatgt taaagtgaca aggagggcgg
     2881 tcgaagaggc aaaatagggc gtttgcatta attctatggg aaacagtcaa ccccgatggt
     2941 ctctatttga tgtgggacca ggggaggggg gcgcccgggg gcttcgggcc gtagacgaag
     3001 acgtccgggt ggacggcggc ctccttgaag gcgcggggcc agttgccgcc gttccggtgg
     3061 gcggccagca ggatcgcccc gacgcgccgg tcgcccagcg agagcagggc ctggaggtag
     3121 gcggcgcggg gcgtgtcgtg gatgacgcgg acggcgcgtt ccgggcgcag ggcggcggcg
     3181 atgcgacgga tcttgtccgc cgtggcgcgg atatccgcga gtggccggcg ctgccagggt
     3241 gtggccgcct tggggataaa ggggttgagg ctcagggtca cccgccggaa cgccctccgc
     3301 gccgtgccgg agcggaccgc ggggccggcc ccgccttcta tccctgtttc ggtttctgca
     3361 tgcagaatcc tccgggtcag cgcgacgatc gcctcgatgt ccacgtccgt ctccgtcggc
     3421 aggccgacga ggaagtacag gcgcaggttg gggatgcgcg ccgcggcgag gcgcccggcg
     3481 gccgcgagga tctgggcctc cgagatcccc ttgcggatca ggtcgcgcag gcgctgggag
     3541 cccgcctccg gggcgagggc gaccgtgtcc acgcccgtct ccttgagcag gccgaccatc
     3601 tcgtcgtcaa ggcggtcgat gcggagtgag ccgacgcccc agcggccgtt ccgggcgagg
     3661 atggcccggc acagggcggg gagttccggg tggtccgaga cggccgtgcc gaggaggccg
     3721 atccgcttgc cggcgtcgat cccccgggcg aaggcgggcg cgagcgtcgc cgcgctccgg
     3781 aagcggggag gccggtagac atagcccgcc gcgcagaagc ggcatccgcg cccgcagccg
     3841 cggctgacct cggtcaggta catctcgccg aattcctgcc ccgccgtgac gatcgcctgc
     3901 tcggccgggt ggcggtcgag atcggcgacg tgacgccgcc ggatgacggc cggcacgtcg
     3961 ccgatgaggc gggaatccgg gccttccttc aacaggccgg ggtcccgccg ccgcgggaat
     4021 gacgatgcga tgcaccgttt cccctccgtt tcgccgtaac gcgccgcata gagcgccggc
     4081 gcatacgccc cgccgacgtc ctgctggatc cggatcagcc gttcgaggcg atcgtccccc
     4141 cgctcgcggc cggcggccca ggtggcgaga aactccggga tcatttcctc accctcgccg
     4201 agcaaaaaaa ggtcgaagaa atcggccagg ggttcggggt tgagggtcac ggcgatcccg
     4261 cccgcgatca cgagcgggtc ggcctccgcg cggtccgcgg cggcgagggg aatgcgcccc
     4321 ccgtcgagga tcttcaggat gttggggtaa tcgttctcga aggggagcga gaaggcgacg
     4381 atgtcgaagg cgttcagggg tttgcgggac tcgagactga gcggtgcggt tgcgccgggg
     4441 atgtccgggg acgcccgccc ggtcttcgcg agaaacgggg gggagggaga gccccgcttc
     4501 acggggtcgg gcagaaagag gcgttcgcac agaaaggcgt ccgacgcgtt gatcaggcga
     4561 tagacgatct ggaagcccag atttgccatg cccgtccggt agacgttggg gtagaccagg
     4621 cataccgtga acgtccggcc ccagaccttc cggacggttc ccgtctcggc ggccagcagg
     4681 gcctcggccc gtctgcggtc ccggtaggtc atggagtcac gtctccgcgc cgttcccgcc
     4741 cgcgccgggg ggaccccgcg cactccgcgg acggaatcga acgttttgca ttcagaaaga
     4801 gcgacggacg cccttttcga aaaacgctca tgttgtaaga cctgaccccc gatccgcccc
     4861 cccgtgtcag tcggcctggc ggcgcaggaa ggtcgggatc tccaggtccg ggttcgcctc
     4921 gtcgtcgatg aggcccatct tgacgacgtt gagcccttcg gccttctcgt tgcgcatgaa
     4981 ggtcggcgtg gcgagatccg cccgcttgta gccgcccatg gacgcgacct tgccgatcgc
     5041 cttcttgccg atcgtgtcct cgaagcccgt cgcgatcacg gtgatgcgga tctcgtccgt
     5101 catggtctcg tccacgacgg tgccgaagat gatgttcgcc tcctcgtggg cctcctgctg
     5161 gatcatggac gacgcctcgt cgatctcgaa gagcgtcatg tccggcccgc ccgtgatgtt
     5221 gagcaggacc ccgcgcgccc cctggatgga gttgtcttcc agcagcggcg acgagatggc
     5281 cttctgcgcc gcctccaccg cccggttctc cccgcccgcg atccccgtcc ccatcagggc
     5341 gaggcccttc tccgacatga tgttcttcac gtcggcgaag tcgaggttga tgagccccgg
     5401 gatgacgatc aggtccgaga tgcccttgac cgcgtggtag aggatgtcgt cggccttctt
     5461 gaaggcgtct aggagcgaga tgttccgccc ccccaggctc aagagccgct ggttcgggac
     5521 gacgatcagc gtgtccacga tctcccgcag ctcctcgatg ccttcttccg cctgcgcgct
     5581 ccgccgcttc ccctcgaagt ggaagggctt cgtgacgacc gccacggtga gcgcgccctg
     5641 ttccttggcg acctgggcaa tgaccggcgc gccgcccgtt cccgtaccgc cgcccagacc
     5701 ggccgtgagg aagatcatgt cggcgccccc gatgtgctcc tggagggcgt cccgcgtctc
     5761 ctcggcggct tggcggcccg tctcgggatc ggagccggcc cccagcccct tcgtcacctc
     5821 ggcgccgagc tggaccttga tgggcgacag ggaggcccgg agggcctggg cgtcggtatt
     5881 ggcggcgatg aagtccactc cctggaggtt gtaggagatc atggtgttca ccgcgttccc
     5941 ccctccgccg ccgatgccga tcaccttgat cctcgccgca tgcaccgcgt tctcgtcaga
     6001 caaaacaaac atagcccctc ccccttagaa gaattctaga aaccatctct tgagactgcc
     6061 gatccagttg ccgaagacgc tccggtcgat ccggaccagg acctcccggt ccagattctg
     6121 actgccgtaa agcagaagac cgacgccggt cgcgtacatg ggactcgcga ccacgtcctt
     6181 cagtccgccg acgcccgtcg gcaggccccg ccggacgggc aggttgaaga tctgttcgcc
     6241 gagctccggc atgccgtgga gcagggcggt tccgcccgtg aggaccaccc ccgcggtcag
     6301 catctcgtcg cagccggact tcgtgatctc cttcatcgcc atgttcagga tctcctccat
     6361 ccggggctcg atgatccggc ccaggatctg cctggagacc tcccggggct cgcgcccgcc
     6421 gacgctcggg acctcgatgg cctcgtcctt cggaatgagc ggcgtgtagg cgcagccgta
     6481 gtggatcttg atcttctcgg cctcggtgac gggggtgcgg agccccgtcg cgatatcgct
     6541 cgtcacgtag ttgccgccca cgggcaggat cgccgtgtgc ttcacgctgc cttcggcgta
     6601 gacgacgatg tccgtcgtgc cgccgccgat gtcgatgagc gcgacgccca agtccttctc
     6661 gtccgggccc agaaccgcct ccgcggaggc gagctgctcg aggacgaggc cgtggatctc
     6721 gaggccgacg cggctgatgg acttggcgat gttttggatg gaggtcgtcg cccccgtcac
     6781 gatgtggacc tttgcctcca ggcggacccc ggacatcccc acggggtcgc ggatgccgtc
     6841 ctgatcgtcc acgatgaagc tttgcggcag ggtgtggagg atctcccgat ccacgggaat
     6901 ggcgatggcc cgcgaggcct cgacggcccg atcgacgtcc tcctccccca cctcgcggcc
     6961 cttcacggcc acgatgccca ggctgttctg gctcttgatg tggctgcccg cgacgccggc
     7021 gaagacggtg ctgatctcgc agccggacat cttctcggcc tcctcgacgg ccttgcggat
     7081 ggactccacc gtgctgtcga tgttgacgac gacgcccttg cgcagtccct cggacgggca
     7141 ggacccgatg ccgatgatgt cgatcccgcc gtcggtcatc tcgccgacga tggcgcaggt
     7201 ctttgtcgtc ccgatatcca gcccgacgat cacgtttccc ttcttcccca tgggtttctc
     7261 cctcgttcag gtccggtaag cctgggccgg ctccgccggc gcgggcggac cgggcaggcg
     7321 gcgcggctgc acggtgatct tcgtcggatc ggcgagatcg atgagcaggt acgccggttt
     7381 gatctgccgc ttctccagat cggccatgac cggtgccaga cgcttgaatt tgttgccgta
     7441 gttgtcgaat cccagccgga agcacaggcc ggcatcggtg tagacggtga agcccgtgac
     7501 ggtgctgccg tggatctccg aaaccgcgcc gtagggggcg aggtctcggg tccgggcgag
     7561 ctcatcgagg agggcgacgg ccctggccgt cagctctgtg ggcatgcgcc cgtccgcgcc
     7621 gccgcccgtc aggacgggga aatccgcctc gtccccggta tcccaccgct tgaaggggac
     7681 cccgtccgtg tccatcagga agagatccgg accctgcttc accatcgcca cgggccggcg
     7741 ctcgcggacg atcaggacga ggcggccggg gagttcccgg cccacgaaga cgtcgcgcac
     7801 ccaggggttc gtccggatcc tccgggcgac cgcggtgcag ttgaccgcga agaggttctg
     7861 acgcggcttc acggcggcca gggcgaggac gtccttctcc gtgagttcgt gaacgccgcg
     7921 gacggaggtt tcccggatca cgagccaggg ggcgctgacg acgaacaggt agatgtatgc
     7981 ggcgaacgcc gtgatcatga aaaccgcccc cagcaggaga aaggcgtaga tcgcgtcgcg
     8041 cgcgagcgtg cccgagcgcc gctgcagtcg gtacttacgc gcctccagcc tgttgttaaa
     8101 cggtttgtgc ttcttcagcc accccatgcc gtttctccga aattaattag ggacagcgcc
     8161 ctattatttg tgcccgatta agtaggcccg catctcgctc catcatttct aggctttccg
     8221 gtcgttccgg gtcaataggg ccttgttcct attcatgcga ttcatttttc ccgccccgcc
     8281 agaggcgtat tccgttgctc cgggactttc cggtcgaaaa atagggcgct gtccctattt
     8341 tccctatttt tcgccgatga tccggacctc cggttcgagg tcgacgccgg cgtgcgcccg
     8401 gacgcgtacc cggatcagct ccatcagggc gatcacgtcc gcggcggtcg ccccgcccag
     8461 gttgacgata aaatttgcat gcatctccga caccatggcc tgtcccaccc gggttccctt
     8521 cagtcccgcc gcctcgatca gccggcccgc cggcgtgggc cgcggattct tgaagatcga
     8581 tccggcgttg cgctgcgcga gcgggtgttt cgtgcgccgc agggccagcg tctcgtccac
     8641 cgcctgccgg atggtccccg cttcccccgg gtgcagggcg aagcgggcgg cggcgattac
     8701 ggcctccgcg gggaggtccg tgctccggta cgagaaggtc agtgcctcgc ggggcgtctc
     8761 ccggagctcg cccgtccgcg acagccacac gacggcggtc aggatgtcgg acaactcccg
     8821 cccataggcc cccgcgttca tccggaccgc cccgcccaca ctgccgggaa tcccggcgag
     8881 gaactccgcc ccggccagtg cctcctttag agccagggcc accagcgcct ggagggcgac
     8941 acccgccccg gcggtgacgg acactgcgcc ggcggccggt tcgcgggttt cgcgtcggct
     9001 tttacgggcc gcaccgtcgg cgccttgagt cgctgcgccg tccccgcgcc gaccggaatc
     9061 cagggcgtct ccgggatcgc cggattcccg cctgggcagg atttccaccc cgggtgggta
     9121 cggacgcgtc ccggacaccg ggggccgctc ccccgtttca cggtcgaccc ggaggtcctg
     9181 caggtccttc aggacgacga tcgcgccccg gacgcccccg tcccggcaga tcaggttcgt
     9241 cccgttgccg accggcgtcg cgggaaggcc cgcctcatgc aaacagcgga ggcagcgggc
     9301 cagatcgtcg aggtccgccg ggaagaccat cacgtccgcc gacccgccca cgcccattga
     9361 ggtgtggcgg gccaggggct cgtcgaacag gacgcgcccg gcgcatgccg ctttcaacct
     9421 ttcccggctt gcctgctcca tccgcctcgc tccccgcgtc cgtttaagta gggacagcgc
     9481 ccaattaatc ttgttctgtt tctgcgttcc accccgccgg gttatttcat cggggacggc
     9541 gcgcaattaa tttattccta atgatcttcg ccatcatccc gtcgttaccg agaaataaat
     9601 aggcgctttc cctatttatt ccgtccctat cgattgagga aggcctcgcc gatcttcagc
     9661 acattgcccg cccccagggt gaggatcacg tccgacggct gcgccgtcct gatcaactcc
     9721 tccacgatcg ccttgaaatc gccgatatac tgcacgtccc ggtggccggc ccggcggatg
     9781 ccgtcggcca gggcggccgc gctcaccccc gggatgggct cttcgctcgc cgcgtagatg
     9841 tccgtcacga tgagccggtc cgcgttgggg aaggccgtca ggaactcctg gaaaagggcc
     9901 ttcgtccgcg tgtagcggtg gggctggaag acgacgatca ggcggtcggg ccacacctga
     9961 cgggccgccg ccagggtctc gcggatttcc gtggggtggt ggccgtaatc gtccacgacg
    10021 gtgacgtcgt tgacccggcc cttcacctcc aggcgccgct gcacgcccgt gaaggtcttg
    10081 agcccggccc gcacggcgct gaaggagacc tccaactcgc gggcggcggc gatcgcggcc
    10141 agggcgttgt agacgttgaa aagccccggg gcgttgaggg tcaccgagcc ctggaggtcg
    10201 ttccgccagt acacggcgaa cttggccgtc cggccggaga agtggatctt ctccgcccgc
    10261 cagtccgcct cgcggtgcag gccgtaggtg atgacccgcc gcttgatccg ggggaggatc
    10321 gcccggacgt tctcgtcgtc caggcagacg atcgtcgccc cgtaaaaggg gacgatgttg
    10381 gcgaactgga gaaacgcatc cttgatctcg gcgaggtcgc ggtagtggtc gaggtgctcc
    10441 cggtcgatgt tcgtgatgac ggcgagcgac ggggagagct tgaggaacga gccgtcgctc
    10501 tcgtcggcct cggcgacgat gacctcgccg tcgcccaagc gggcgttgct cccgatggag
    10561 gcgagtttgc cgccgatgac catcgtcggg tccaggccgc cccgggccag gatggtcgca
    10621 atcatcgagg tggtcgaggt cttgccgtgg ctcccggaga cggcaacgga aaacttcatc
    10681 ttgaggagtt ccgccagcat ctcggcccgc gggatgacgg ggacgccccg ccgcttcgcc
    10741 tccaggacct cggagttgtc cggccggacc gccgtggagg tgacgacgac atccgccgcc
    10801 ccgacgtggg cggcgccgtg gccctggtgg atggtcgcgc ccaggtcgac caggcgctcc
    10861 gtaatgtccg tccggttctg gtcggagccg gtgacggcgt agccgaggtt caggaggacc
    10921 tcggcgatcc cgctcatgcc gatcccgccg atgccgacga agtggatctg ccggaccttg
    10981 cggcgcatca tatcggtttt cttgctgttc ttctgttttc gcatcgaaac gtcccatgtt
    11041 cgcccgggga tcgggcgcaa atcaacacac tttatatggt atgtggccgg gctctatata
    11101 ccatatctgg tgccatatcc accgaaaagt ggccttccgc ctgcgcaata tccggggctt
    11161 cccccttgcc cctttttccc ccgccccgct catgtttgca ggagcgccag acacgcgtcg
    11221 acgatgtccc gggccgcgtg ggtgttcccc agggccgccg accgagtcgc catcccgtgg
    11281 agcgccgccg gatcgtcgac gtaacggcgg accgtcgccg cgagccgcgc ggccgtcagg
    11341 tcgcgctccg ggatcagctc cgccgcaccc gcccgggcga ggacctcggc gttgcgcgtc
    11401 tggtggtcgg cgatggcgta ggggaagggg atcaggatcg ccgcctttcc catcgccgta
    11461 atctccgcga tggatgtggc ccccgcgcgg cagacgatca ggtccgccgc cgccgcgacc
    11521 gcggccatat ccatgatgaa ggaccggatg tcggcctccc gccctgcgtc ccgataggcc
    11581 tggcgtacgg cctcgagatc cgccggtccc gtctggtgga tgatgttcag atccgccagg
    11641 tcccgccagg ccgaaacggc ctccatcacc gtccggttga tcgcgtgcgc cccctggctt
    11701 ccgccgaaga tgaggagcgt gaaggggtgt cccggtgcgg tgtgccccgt gccctcgttg
    11761 tgccggaact cgaccgccgc cgtgcctttt tcccgcacgg cctctccggt tgcggcgaag
    11821 gccgcccgga cggggttgcc cgtcacccgg atttttgccg gcgggaactc gccccgttcg
    11881 tcggcgaagg tgaggaagat ccggtccgca aaacgggcga ggatccggtt cgtcgcgccg
    11941 gggatggcgt tctgctcggc gatcgccgtg gggagaccca tccagtgcgc catgaggacg
    12001 gccgggccgg aggcgtagcc gcccacgccg acgacgacgt ccgggccgaa ttgccggagg
    12061 atccggcgcg actgggcgag gctccgcggg atcttcgcca gggcgccgaa tttctgcgcg
    12121 aggccgcgtc ccttgacgcc ctcgacgtcc agggtttcga gcgtgtagcc catctgggaa
    12181 agaacgcgct tctccaggcc gcgctccgtg ccgacgaaga gcacccggtt cgcggggtcg
    12241 cggcccagga attcctcggc gatcgcgatg ccggggaaca ggtggccgcc cgtcccgccc
    12301 cccgcaacga tcatcttcct cccgccgccc atgtcaggac tcctgcgccg agatgttgag
    12361 cagaatgccc accgccgcca ggctcatcaa aagcgccgag ccgccgtagc tcaggaacgg
    12421 gagggccagc cccttgagcg ggatgagccc catcaccccc gcgatgttga tgaaggcctc
    12481 cagggcgagc gtcatcgtga ggccggcggc gagcagggtc ccgaagaggt ccggggcctt
    12541 gaaggcgatg aagaaccccc ggatgatcag gacgccgaac agcaggatca cgatggccac
    12601 gccgatgaag ccgctctcct cggcaatcac ggacaggatg aagtccgtgt gcggctcggg
    12661 cagatagaag agcttctgca tcccgtcgcc caggccgacg ccgaaggttc cccccgagcc
    12721 gaaggacagg agcgactgga tgatctggaa gcccgagttg cgcgggtcct tccaggggtc
    12781 gagaaaggtc atcaggcggg ccatccgata gctcttgtgg atgacgaggt acagggccac
    12841 gggcaggaag gccgcggcca ttccgccgag atagacgagc cgcgtccccg ccaggtagag
    12901 catcaggagc aggatcgcca tgaggatcgc cgccgagccg aaatcgggtt ggagcatgac
    12961 gaggccgacg atgaagagcg tcacggcgag cgggatggcg atgccgcggc tgaaccgctt
    13021 gatctgggcg atcttgcgcg tcaggaaatg ggcgaggaag acgaccagtg agactttcac
    13081 gagttccgtg acctggaagg tgatgatccc caggttcagc cagcgggtgg ccccgccggc
    13141 cttgaccccc acgtggggga tcaggatgag ccccaggagg accgccgccg cggcgagacc
    13201 cggataggcc gcgtagcgga gcttctgata gggtaccttc gtgagaaaga ccatggccgt
    13261 caggccgagc aggacgaaga agatctgctt cttcaggaaa tactgggcgt cctggtagcg
    13321 ctccatcgcc aggatggagc tcgaactgta gatcatgacc gtgcccaccg tcacgagcag
    13381 gagcgtgctg acgagtagga ggacatcagg gcgtttgtcg gtttcggtca gtttgaacat
    13441 cgtctacagc cccttgaccc agcgctgaaa catatccccc cgctcccggt agtcggtgaa
    13501 ctcgtcgaag ctcgcgcatc cgggggagag caggacgacg tcgcccgccg ccgcagcctc
    13561 gcgggcggcc tccaggcccg ccttcagggt ggcggcgcgc cgggtggcga cgatctcgcc
    13621 gatgaggccg ccgatgcgct cacgggcctc gccgaagagg atcaacgtct tgacgcgctc
    13681 gcggatgatc ggaatgaggg cggcgaagtc cccctccttg tcgcgaccgc ccagaagcag
    13741 cacgaccggg cgggagaaag tttcaagcgc ccgcgcgacc gcacccacgt tcgtgccctt
    13801 cgaatcgtcg tagaaggcga tgccgtcctt ctctccggcg aactggatcc ggtgggcgat
    13861 ccccgagaag gcggcgatgc cctggcggat cgcctcgggc gggcaacccg cgcggcggca
    13921 ggcgaggatt gcggccatga cgttctcgac gttgtggagg ccgtgcaggc ggatcaggtc
    13981 acgtgcgtaa agttcgatcg cgccgtcggg ggcggcgtag acgaggttgt ccccgtccag
    14041 cgaaatcccg gggcggcagg gtccggaggt ccggaagtat tcaacctcgg cccggaggcg
    14101 ggcggcgagc gccggcgtgc cgggatcgtc cgcattcagg atcgcgagat cccccggttc
    14161 ctgacgagag aagaggcgct ccttggcctc ccggtaggcg gagaaactgc cgtggtagtc
    14221 cacgtggtcg caggtcacgt tgagcaggat cccgatggcg gggcggaagg tctccgtcca
    14281 atgaagctgg aagctgctca cctcgacgac cgccgtctct tcctcctggg ggccgtcggc
    14341 gaagccgata agcgggcgcc cgatgttgcc gccgacgaag gcggtcttcc cggacgcctt
    14401 gaggaggtcg cccacgagcg tcgtcacggt cgtcttgccg ttcgtgcccg tgatggcgat
    14461 catcggtccc ctgaggtggc gggcggcgag ttcgagttcg ctcaggatcg gaatgccgcg
    14521 cgcgccggcc tccacgagca ggggattcga cggggcgacg ccgggcgagg ggacgacgag
    14581 gtcgacgccg tccagggcgg ccggggcgta ggggcggatt tcaggcgtgt cggcggtgga
    14641 ttgcacctcg gcgagggcgg cggtccaagc ggccggaggc ttctcgtccg tgagcgcgag
    14701 gagcgccccg cgccgtccgg caaacgtcgc ggccgcaacg cccgtcttgc ccatcccgat
    14761 gatcaggatc cgttttcctt cgagttccat cgcccctacc tgatcttcag cgtgctcatg
    14821 gccagcaagg ccaggaggat cgagatgatc cagaaccgga cgatcacctt cggttccggc
    14881 catcccttga gttcgaaatg gtggtgaatc ggggccatgc ggaagatccg atgcccgtgc
    14941 gaaatcttga accagccgac ctggaagatg acggagaagg tttccaggac gaagatcccg
    15001 ccgacgatcg cgagcaggat ttcctgcttc gtcacgacgg cgaccgtccc cagcgcgccg
    15061 cccagcgaca gcgagcccac gtctcccatg aagacctgcg cggggtaggt gttgtaccac
    15121 agaaagccga tgcccgcccc cacgacggcc ccgcagaaga cggcgagctc ccccgtcccc
    15181 gcgacgtagg ggagctggag atagcccgag atcttgatat tgccggcgaa gtacgcgaac
    15241 agcagatagg tgatgaagca ggtgatggcc gggccgatgg cgagcccgtc cagaccgtcg
    15301 gtcagattga cggcgttggc cgcccccacg atgatgaaga cggacaggag gatgtagccc
    15361 caccccaggt cgggcaggac ggtcttgaag aagggaatcg tgaccaggga ggagaagtcg
    15421 ggcttgacgt agaggatcac gccgacgaac agggcgatgg cgatttcgcc ggcgaggcgg
    15481 acttttcccg gcaccccctt gctgttcgac cgcgtgagct tccggtagtc gtccaggaag
    15541 ccgatcaggc cgtaccccag aaagacgagc agcacgagcc agatgtactg gacggtcagg
    15601 ttggcccaca ggagcgtcga gatgacgacg gcgaagatga tcaggatgcc gcccatcgtc
    15661 ggcgtgccct gcttgctcag gtggctgttc gggccgtctt cccggatggg ctggccgatc
    15721 cgcatggcct gaagcttctg gatgagccag gggccgacga tgaagcagat gacgagcgcc
    15781 gtcacggtgg cgtagatcgt ccggaacgtg atgtagcgga acacgttgaa gaacgagtaa
    15841 tcgatgtgca gcggatacag caggtggaac agcaccggtt ctctcctttg ggcaaagggt
    15901 gaaaggctta agggcggaaa tccgcccgcc gcctttcacc gtcgccgtgt gcactttgcc
    15961 cgtttatgat gcggggagcg actccccggt ctcatgccga cgccccgttg gacgggggct
    16021 cgtggccttt cgtctttgat gtccgccggt tccatggggc ctgtcccccg tcaaagggtt
    16081 tctcggatcg cctgcacgac cgcatcgagg cgcatccggc gcgatccttt gaccaaaatc
    16141 caatcccccg cgtggaggcg atccttcaaa tccgcgacga cctcctcggg cgacgtgaag
    16201 aaaacgatcc gttcgcgggc caggcccgcc gcaacggccg catccgccgt cgtccgggcg
    16261 aacgcccccg tgaggtggac ctgcgagaca cccgtctcta ccaggcgcgc gccgatttct
    16321 gcatgcaggt tttccgactg ttcgcccagt tccagcatgt cgccgaggac cgcgacgccc
    16381 gcgccactct cccggagtcc cgccagcgtc tgcagggccg cggcgacaga ggcggggttc
    16441 gcgttgtacg cgtcgtcgat caggtgcgca ccgctggcca gggtgaagat ctgcatgcgg
    16501 ccggcaacgg ggcggaaccg ggccaggccc gccgcgatcg tgtccgggtc cgtcccggcc
    16561 gcccaagcca gggccgcggc ggccagggcg ttggcgacgt tgtggcgtcc gggcgcggca
    16621 agggtcacct cccgtgcggc gccgcccgcg tgcagggtga agcgcgtccc gccgcccgcc
    16681 gccggcgccg gggcgtcggc gaacacgtcg tgcgcggggt cgaaaccgaa aacgaccgtc
    16741 cgtcccggcc agcgctcctt cagggcgtcg cgggaggggt catccccgtt caggacggcg
    16801 attcccccgg cgggatccat ccggccgaaa aggtcaccct tctcccggcg gatggcgtcg
    16861 agcgacccca gcccttcgag gtgggcgggg ccgatgttgg tgacgaggcc cgcggtcggc
    16921 gcggcgatgt cggtcaggcg ggcgatctcg cccggccggt tcgttcccag ttcgaggacg
    16981 gcgatctcgt ccgccgcgtc gagtctgagg agggtcaggg gcagcccgat caggttgttg
    17041 aagttcccct cggtcgccag gacccgcttg ttctgactga ggatggcatg ggccatctcc
    17101 ttggtcgtcg tcttgcccga actgccggta atcgcgcaga cgggcagggc gaagcgcatc
    17161 cgccaggcgt gggcaatccc gccgagggcg gtcagggggt cgtccacggc gatgatgacg
    17221 atcccgccgg gcgcgccggg gagacggtcc cgccgggcgc gggagacgag cagcccgccg
    17281 gcgccccgtg cgagggcctc gccgaggcag tcgtggccgt tgaagtgctc gcccgcaagc
    17341 gccacgaaga gggcgccggg cgagagcgtc cgggtgtcgg ttgaaacgcc ggcgagaacc
    17401 gcctccggcg ggccggcgat caactcgccc ccggtggcgg cgaggatcgc cgcgacgtcg
    17461 agcctcgggc cgccgcgttc cgtgtcgccg ccggtccggg cctcggcgag cggacgcttc
    17521 cgggcgtcga gggccctacg ggcaatgagg cggtcgtcga agaagatccg gcgatcgccg
    17581 aggatctggt agtcctcgtg gcccttgccg gcgatgaggc agatgtccgt ctccgccgcg
    17641 agcgcgatgg cggcggcgat ggcctcggcg cggtcgggaa tcaccgtgta gcccctgggc
    17701 gatgccgtcc ccggcgcctc gggactcggg gtgctggtca tcctgggttg gcggtccggc
    17761 ttcagggcct ccggaggcag ctttgggatg ccgcgggcgg cgatgcccgc ttcgatctgg
    17821 acgatgatgg ccgcggggtc ttcggagcgc gggttgtcgg aggtgacgac ggtcaggtcg
    17881 gagaggtccg ccgccgcccg gcccatgagc ggccgtttcg cccggtcccg gtcgccgccg
    17941 cacccgaaga ccgtgacgat ccggccccgg cgatggtcgt tcagggtctc gaggacgcgc
    18001 cgcagcgcat cgtccgtgtg ggcgtaatcg acaaacaccg tgggctcgcc gccgcggctg
    18061 acccgctcca gacgccccgg cacctgggcc agggccgcga tcccggccgc gatcgcgtcc
    18121 gtggggacgc ccaggctcag cgccgcggcc gcggccgcca gcaggttcga caggttgaac
    18181 cgtccgatga ggggggagcg gacggggaat tccccctggt cgcatcgcag tcgggcggtg
    18241 atgcccgcga gggtgagctg cgcatccgcg gccgtcaccc ccgccctctc cgtgaggccg
    18301 aacgacagga cggggagggt gacctcgccc gcgagccggc gtccccaggg gtcgtcggcg
    18361 ttgacgacgc aggggcaagg ccgtcccttg ccgccggagg ggaggacctc cgtaaaaaaa
    18421 cgccgcttgg cggcgtagta gcgctccatc gtgacatggt agtccaggtg gtcctgcgag
    18481 aggttggtga agacgccgat atcgaaggcg acctcgtcca cgcgtttcaa gtccacggcg
    18541 tgcgaggaga cctcggcgac gacgtgggtg acgcccgcgt cggccatctg gcggagaagg
    18601 cgctgcagct cgcaggattc cggggtcgtg ttgggggcgg cgaaggtctt gtcgccgtag
    18661 cggtagttga ccgtgcccag gacccccacg cgatagccgg cggccttgag aatcgcctcg
    18721 aggaggtagg tgacggtcgt cttgccgttc gtgccggtaa tggcgacgag ggtgagggcg
    18781 gccgacggat ccccgaagaa gtttcgggcc agccgcccca gggcgcggcg ggcgtcggcg
    18841 acccggaccg ccgtcacccc ggacggaggc gtccacggcg cttcgtgaac cacgacccac
    18901 gccccccgcc cgagcgcatc ggcgatgtgg gcgtgtccgt cggtcttgag tcccgggatg
    18961 gcgacgaaga gcgatccggg tccgcaggtg cgggagtcgt agcagaggct ttcgcatgtg
    19021 ccggacaggt tgccccggat ctccaggagg tcaaccgtat ggaggaggcg agtcagatcc
    19081 acattccccc cctcagtccc cggtgtgaaa ggaaacggtg cagagccgtt caccgaccag
    19141 gggcgtgccg ggcgagggcg cctgattcag ggcccagccg ctgccgagga cgcgcaaccg
    19201 gatgtttctc tcccgggctc gcttcaaggc gtcccggatg gtgagccccc ggaaatcggg
    19261 catgacggct tcgtcgatcg tgctttcctc cgccccgctc acggcggtgc cggggatggg
    19321 cagggtccgg gaggccgaga ccagcgtgag ccggtgcgcc ggtggcgcac tgtcctgcgg
    19381 ggacggcgcc ggggcggggg attcgcgcag gtggctcttg aagcaggcga ggatctgctc
    19441 gccgatgccc ttgaacaccg gtgcggcggc cacgcccccc catttgtccc gctggggctc
    19501 gtccagggtg atcaggatgg cgacctgcgg gtcttcggcg gggaagaagc ccatgaagga
    19561 ggtccgcacc cgctccgaag agtacacgtg acgggccgtg tcgtacttct gcgaggtgcc
    19621 cgtcttgccg gccacgctca cgttttcgat ccgggcccgc ttccccgtgc cgtcctcttc
    19681 cccaacgacg tcggtcagca tggcgccaag gcgtcgggcc gtggcgggcg agacgacccg
    19741 gcggaccggt tggggcgaat tggactggat gatctggccc ttccggtcca cgatggcgcg
    19801 gacgatgtag ggcttcatga ggacgccccc gttggcgacg gcggacaggg ccgtgatgag
    19861 ttggatggcc gtcaccgaga cgccctgtcc gaaggccacg gtggaggcgt ccacgcgcgt
    19921 ccagttctga accgggcgga ggagccccgg gacctcgccg ggcaggtcga tgccggtctt
    19981 ggccccgaag ccgaattttt gaatgtattc gtagaatttt tccttgccca gccgctcgga
    20041 gatcttgatg gaaccgatgt tgctcgaata cttcaggatg tcgtgcaccg tgagcgttcc
    20101 gtgacgtttg cgctgggcct cgtggaacac ccggtcggcg acggcgtagg cgccgttttc
    20161 acagtagaaa cggtcgttct cccggacgac ccgctcctcg agggccgccg ccacgaggaa
    20221 gggcttgaag gtggagccgg ggtcgtaaca gtccgtgatg gccttgttct tccagacctg
    20281 atggccgaac ttgccgaagt tgttggggtc gaaggtcatc tggttggcca gcgcgagaat
    20341 ttctccggtc cgggggtcca tgacgatggc gtagccgcct ttcgcgccct tgtccaggac
    20401 tgcctccttc aggcgctgct ccgccaggta ctggattcgg ctgtcaatcg tcaggatcag
    20461 gttccggctt tcctcggggg tgacggcgac ggtctccaca cgggcgtaca ggggcttgcc
    20521 cttggcgtcg cgggcccggg tgagcttccg gggggcgccc atcaggtggc ggtcgtagag
    20581 catctcgatc ccttccagac cggaggcgtc cgtcccgacg aatcccagga ggtggccggc
    20641 gagttcgccg ctcgggtaga aacgcttcgg ctcctggatg aggaagacgc cgtcgatatt
    20701 gagggccacg acccgggccg cccggtcggg cggaatgcgc cgtgcgatcc agcagaagtt
    20761 tcccttgcgg gacagcttgc gccggatctg tccggcatcc tggtccagga tcgcggcgag
    20821 tttcgccgcg gtgtcgcccg cccgggtgat cttggccgga tcagcgcaga cgctgtccgc
    20881 catcacggag acggccagct tttcgccgtt gcggtcgaag atgatccccc gctccggcgg
    20941 tacctgcagg gtcttgacgt gctggcgctc cgcaagcgat ttcagggtcg tgccggacag
    21001 gatctgaagc tggaaggccc gtgaaacgag cgcgatgaaa agcacgagga agacggccat
    21061 caggctcgcc agacgaaatc gcagccattt cttcgcttcc cggttcatgg cgtctcctcc
    21121 ccggtcttca gcatgacgac ctgctccagg gtcggcgcgg tcatctggag gcgatcgcgg
    21181 gcgatggctt cgatccgccg gggcgccttc agggtggcca gttccagcct cagccgccgc
    21241 tgctcctcga tgagcgtctc cttctggttg agtgcctcgg cgacccggta ctccagggtc
    21301 gtcatgtgga tgtggcacca cacgtacgcc agtgcgacgg ccatgaagac aaaggccccg
    21361 acgatcagga cggaatgggg cggccagcgg gaggcgggtt tctcctccgg cagccggtcc
    21421 tttttgggcg cgtccggagc ggtcatggtg cggtcctctc gatggttcgc agacgggcgc
    21481 tgcgcgcccg cggatttcgg tcgatttccg cgctgtcggg ggcgatgggc ttccggtgga
    21541 tcgaacgggc ggtcggcgtg agtccgcagg cgcacaccgg gagtccgggc gggcagatgc
    21601 agcccttttc ccaggtgcgg aaggtttcct tcacgatccg gtcttcgagg gagtgaaacg
    21661 tgatgacgga caggcggccg ccggggcgga gtcggtcacg gcattggggc agggcctgcc
    21721 tgagccgggt cagttcgtcg ttgacggcaa tgcggagggc ctggaaggta cgtgtggccg
    21781 ggtggatccg gcggtggcgc agggggggag ggacggcggc gcagacgatg cgggccagtt
    21841 ccgcggtggt cgcgatcggc gcctgtcggc gtcctgccac gatcgctctg acaattcttc
    21901 ccgccatgat ttcttcaccg tactctctga tgatccgctc cagctccgac gcggaggcgc
    21961 tggagaggag ttcctgggcc gtgatccccc ggctgcgatc catgcgcatg tccaggggcg
    22021 ccgggcggga aaagctgaat ccccgcgacg ccgtatccaa ctggtgtgaa gaaaccccca
    22081 aatcgagaag aacaccgtcc gccggccccg ggtgtcgatc cgacagcagg gcctcaagcc
    22141 gggcaaaatt gccgtgaatc aggagcgtcc ggtcgcgaaa gggcgccagc cgttcccggg
    22201 ctgccgtcag ggcttcctcg tcctggtcga tgccgatcag gaatccgtcc ggggccgtcc
    22261 gcgccaggat cgccgcggcg tgaccgccgc ctccgagggt gccgtcgatg tagatgcctc
    22321 cccgccggca gttgagcgag gcgaccacct ccccgagcat gaccggtgcg tgaaagcatt
    22381 ccatgattta cggccgtcgt ttaaatgccc aattccgcca tgcattcgct cagggtgtcc
    22441 ggatcgcctc ccgttttggc catctcggcc tcgaagcgct ccttgctcca tatctcaatg
    22501 atcctgatca tgccggccag aaccacgtcc ttctccaggt gtgcgtattc ccgcagattc
    22561 gggggaatca gcacccgccc ctggccgtcg atcgcgcagt ccacggcccc ggacataaaa
    22621 aagcgctgga aagcccgcac ttccttcttc aacagggact ggaagctgac cttctcttcg
    22681 atgatccgcc attcttcgta ggggaacacc atcagatacc cgtcccagtt cgtgatgacc
    22741 aggcggttgt cgtacttctc cgcgaggacc tcgcgcaggc gggaagggaa gatgatccgt
    22801 cccttgtcgt cgatggtatg atgatactgc ccccggaaga cagacatctg ggaaagtcct
    22861 ccactaaaaa ccacttcgct ccactttggg gcaggtatag aggaggattt tggctttgtc
    22921 aagaaaaaat tttaattctt tttcctgtca gtttgatatt ttacgatggc ttgccgatgg
    22981 gcgaggaggg tggaggaaaa ataatgcgac ccgttattgt tcgagacgaa gtagaggtaa
    23041 tcgaccgggg ccggttccag ggcggccttg agggagtcga ggccggggtt ggcgatgggg
    23101 ccagggggca accccgcgat ccaataggtg ttatggggga cccggcgccg gaggtggcgg
    23161 cgggtgatgg ttccgttgaa atgggcgagg tcgtagacgg cggtgggatc gctctggagc
    23221 ttcatccctt ttttcaggcg gttgtgaaag acggcggaga tcaggggctt ttccgaccgg
    23281 cacccggatt ccttgccgat cagggaggcc agggtgaccc attcccccat cgtcatgccc
    23341 agcgcctccg cccgcacgat catgtccggc gtcacccgtt tcctgaattc cctgaccatg
    23401 aggcgcatga tttcacgttg ggacatcgtg cggtcgagca ggtaggtgtc cggaaacagg
    23461 tacccttccg ccgaggccgc gtcgatgtcg agcgaagcga ggaatttccg gtcatgcgcg
    23521 agcgcgatga actgctccgg atcagccagt ttgtgctcgg ccagtcgttc ggcgatttcc
    23581 ctcagggtca ggtcctccgg gatcgtgacc cggtggtgtt tgatttcgcc cttgacgagc
    23641 tttttgacga tctggcgggg cgtcatcgcg gtggtgaatt cgtattcgcc ggcccggatc
    23701 tggcggtggg cctgcttgcc gatgacgagc acgtagtaca gggggcggtt cttgatcagc
    23761 ccggcccggt ccaggatgtc cgtggccccg aaaaagcccg ttccctgggg gatgtccacg
    23821 gtgacggttc gcgtttcggg ggcgacggcg gtgttggtgt ggtagacgta gaacggaacg
    23881 aacagggcga gcaggaggat aatcgtcccc gcagtcctcc agacacgatg tttgaaagac
    23941 ttccgcttac tcatggatcg tcccgctttc ggtccggctt cggcatgcat tgaggtagcc
    24001 ctgcaggatc agggaggcag cgacgcggtc cacgctccgc cgccgtcttt ccctccgcag
    24061 tccggcctcc cgcatcaggt cttcggcctc ccgggtcgtc agggtttcgt cctgtcggat
    24121 gacgggcagg tcgaaggacg cccgcagggt ggcggcaaat cggttcacct tcgcgcactg
    24181 gacggcttcc gagccgtcga ggcggatggg gtagcccagg acgatccgtt ccaccccgta
    24241 gcgggcgatg agatcgccga tggcctccag gacttgccga cggttcttcc gcacgatcgt
    24301 gggaaggccc tgcgcggtca gtccgagttc gtcgctcagc gcgatgccga tccgcttttc
    24361 gccgtaatcc atccccataa tccgcatgtg gtcctctgtc gcgtagacgc gcccgacttc
    24421 aattgcgccg accgcgcgct tcgccccggt gggctccacc ggaccagacg cccccgggct
    24481 gcggcggtgt tcgaggaaat cgaaggctga agaaggggtg tcgcggggac cgcagggacg
    24541 acgggtacgc tgtcgggcgc ctcttttatc cgatttgtcc gccccgtgca agcaaaatat
    24601 cccttttccc attgtgtaac ggcggcgcat ttgctatcgt gggcgcgctt cggacgggaa
    24661 aaacaggaca ggatcgcggt tatatgaaac acctgatcgt cggcaccgcc ggccatgtgg
    24721 accacggcaa aaccgccttg gtccgcgccc tgaccggcgt tgatacggac cgcctcaagg
    24781 aggaaaagaa ccgcgggatc acgatcgaac tggggttcgc gcctctgacg ctgccaagcg
    24841 ggctggcggt ggacatcgtc gatgtgcccg gccatgaacg cttcgtccgt cagatggtgg
    24901 cgggggcggc gggcatcgat ctggtcgtcc tcgtgatcgc cgccgacgag ggggtgatgc
    24961 cccagacccg ggagcacctg cagatctgcg cgctcctggg catccgccgg gggctcgtgg
    25021 cgctgaccaa aacggacatg gtcgatccgg actggcggac gctggtcgcc gaggatgtcc
    25081 gagagtttct ccaggggacc tttctcgaag gggcgccggt ctgtccggtc tccgccctcg
    25141 cgggggaggg gctcggggac ctgctgggtg ccctggacgc cgtggcggcc gccgtcgagg
    25201 aggaggagga ctgcggtctc ttccgccttc ccgtcgaccg ggtgttcacg atgaagggct
    25261 tcggaaccgt cgtcacgggg tccctgatct ccggcgccat cgcggccgga gaggcggtgg
    25321 acatctttcc ccggggcgtg acggcgaaga tccgcgggat gcagatccat aacgagggcg
    25381 tgacggaggc ggcggcgggg cagcgggtgg cgatcaacct ccagggggtg gagaaggcga
    25441 tgctcggccg tggcgatgtc ctggcccgtc cggcgaccct gctgccctcc cggcgtctgg
    25501 acgtgcgggc gtcgtccctt gcctcagccg gccgggcgct caagaaccgc gcccttgtgc
    25561 gcttccacgc cgggacgagc gagctcatgg cccggatcat cctgcgagac cgggacgaac
    25621 tgaagccggg cgaagcggcg gcggcgcagc tctttcttga agcccccgcc tgctgtgtcg
    25681 ccggcgaccg gtttgtcctg cggagctatt cgcccgtgac gaccgtcggc ggcggcgtcg
    25741 tcctcgatcc cctgccgaag aagcaccggc gtcgcgatcc cgaggtcgca tccgccctga
    25801 ccgttctggc ggagggcgac ggcgcggcgc ggacggccgt catcctggag cggtccggcg
    25861 cccggggggc ggcgccgggc gaactctgcg tccggacggg gatgccggtc cgccggctcc
    25921 agaagatcct ggacggcctg ttcgccgccc accgggcggt gctcgtcgac cgcgacgaga
    25981 tgcgggccgt cgcggccggg gtttatcagg acctccagaa ccggatcgtc gcggacctga
    26041 ccgactacca cgcccgtcat gccctgaaag agggcctttc cagggaagag ctgcgcacgg
    26101 cgctgggccg cgccgtcacc ccgaagctct tccagatggt cctccgcgat ctggaaaagg
    26161 cggggcggat cgttgccgac cgcgagcacg tgcgccttcc cggccaccag gtccaactcg
    26221 ggggggaact ggcggcgctc cgtcaggaga tgggtgatct ctaccgcgac gcgggtctgg
    26281 cgccgcccac gctccgggag atccgcgagc gcttcgccga ccgggttcgg cccgcggaga
    26341 gcgtcctcaa ggtgatgctc caggaggggg aactggtcaa ggtcagcgag gatctctact
    26401 accacgggga caacctggcg gccctccggg agcggtaccg tgtgctgctc atccgcgacg
    26461 ggaaggccac gccggtggag ttcaaggagc tgacggggct gacgcggaag ttcatcatcc
    26521 ccctcatgga gtatttcgat ctgaccaaac tgacgatccg ctcgggcgac cagcgtctgc
    26581 tccgggaaaa ggaggggaaa tgacggcatt ggctgtctcg gccttcctga cgtttcccgt
    26641 tcaagtccag gacggccggg atcggatcga aacgtccgcg aagattgtcc gagagatccc
    26701 gctcgaggtg tttctcgacg ggcgacggat ggggacgatc gcgtgcagcg gcctccaccc
    26761 ggaggagctg gcgttgggct tcctgcgcgc cgaggggctg cttgaggacc gacgggagct
    26821 gacgcggatc gaaaccgacg ccggcggacg ggtcgtgcgc gtgcaccggc ggagggaaac
    26881 ggccgctccg gcggtcgcgc ccgagggtca aaccatcgga tccagcggcg cgcgcgggct
    26941 cggcctcgcg gcttcaccgg ccccgctgca tgacatcgac ctggcggtca cgccggcgca
    27001 ggtcctggcc ctgatggacg tgcttctgaa cgggtcggcg atccacaatc tgacccacgg
    27061 gacccactgt tccgcgctgg cgacgccgga ggggatcgcc gtcctgcggg aagacatcgg
    27121 ccgccataac accctggaca tgctcggggg gtacatgttc ttgaacgaca tccccgggcg
    27181 cggccggatc ctcctcacga cggggagagt gtcggcggag atcgtccaga aggtctggcg
    27241 gctgggcgtc cccatcctca tctcccattc cgcgccgaca tcgcgcgccc tggagctgct
    27301 cgcggaggcc ggcatcaccc tgatcgggtt tgtccgggga ggcaggatga acctctatac
    27361 ggaaggaaag cgggtgatcc ggtaatgacg aaatccatct atatcaagga catcaaggcg
    27421 ggagacaagg tcagcacggc cttcctggtg gcggagaaga acatggccta ctcccagaag
    27481 ggggcgccct acctgaatct gaagctgaag gaccggacgg gcgaggtgga tgccaaggtc
    27541 tgggacaacg ccccccagtg ggacccggtc ttcggcaagg gggatatcgt ccactgccag
    27601 gcccgggcgg tgagctacaa gaatgccctg cagctctcga tcctggagat ccgccggctg
    27661 gaggccggcg acgtcgaccc cgcggacttc tttccctgcg cccgccggga cccggaggcg
    27721 atgttcgccg aactggaggg gctcgtcgcc tcggtgaccg ctccgcacct gaaggcgctg
    27781 ctcgcggcct tcttcgcgga cgcggaggtc gtgggggcct tcaaacgggc gccggcggcc
    27841 aagggattcc accacgtcta catcgggggg cttctggagc acaccctgtc ggtggtccgc
    27901 ctgctcgacg tcgcgagccg gcattacgat gggcttgacc gtgacctgct cctcgtgggc
    27961 gggatcctcc acgatatcgg gaaaatccgc gagtaccgct acgagggcct gatcgattat
    28021 tccgacgagg ggcgcctgat cggccacatc gtcatcggcg tcgagatgct ggatgaaaag
    28081 atcgccacgc tggcggcgtt tccgccggag acggcgatgg ccctgcgcca catcctcctg
    28141 agccaccacg gcgagatgga attcggctcc ccgaagaggc ccaagacgct ggaggccatg
    28201 atcgtccacc agctggacga cctcgacgcc aaggtcaacg cgatccaggg gttcgtcgcc
    28261 ggcaccccgg atgacgcctc cgcctggacc cccttccacc gcctcttcgg ccgttttctc
    28321 tacaagggca aacccggctg aacggtgctg acgggcgcac gccggatgtc cgatgccccg
    28381 gcattcaaca ttttttcggc ccggtgcctc ctcgcgcagc gcataggcac acctcgcgcc
    28441 tccggcgctc aaacagtcgc ctttatggac gcttcgctgc gggggaagcg tgcccgaaaa
    28501 aatctcgatc cgcgctgacg gaatcgggga tcgcatggca gacggcacag ggggcctgtc
    28561 caacccgctt cagagggaat tcctgaccca cccccgttca gatggcctcg atggtggagg
    28621 gcggcttctt cgtgatgttg taggtcacgc tgaccacccc gggcacctcc gtcgtgatcc
    28681 ggtccgccag cccctcgagg acctcgtagg gtagccgagt gggggatccc gtccgggcgt
    28741 ccgtactgtt ccagcaacgc agctcgatct ggcagccgta atcccgcgcc ccgtggcgca
    28801 tgcccgtcac gcggtccgca tgcagaatgg cgaggtactg gaacgccccg ctcttgcaga
    28861 gggcctcctc tacgatccgg gtcgcctggc ggacggtgga cacgcgctcc ggcgtgacct
    28921 cgccgatcac gcgggcggcc agggcggggc cggggaaggg ggggcggttg aacaggtcct
    28981 cgggcaggcc gagctcccgg cccacgaggc gcacgccggg cttgcgcagg cggatcagcg
    29041 gttccaggac cttgtacccg aaggcggcct ccgtgtcgat gccgagctgg gaaaggatgt
    29101 tgtgctggcg cttcacccct gcgaccgtct cctccacgtc ggtgaaaatc gagccctgga
    29161 ggaggtggcg cgccccgctt tccttgacga gccggccgaa gaccgccttg tagaaggtct
    29221 gggtgatggc ctcccgtttt tcctccgggt ccgtcaggcc cttcagggct tcgaagaatt
    29281 ccgcccgggc gtcgacaatc tcgaccggca ccttcatggc ggcgaaggcc tcttgaatct
    29341 ggcggggttc gtcttcccgc atgatgccgt tgtcgatgaa gtaggtcttc agacgatctc
    29401 ccagggcccg gtggcccagg agcgtcacca cggacgagtc cacgcctccc gacagggcgt
    29461 tgacggcggc gccgtctccc accgcggcgt tgatggcccg gacctgttcg gcgatgaagc
    29521 ctcgggggtc cagttccgac aggctgattt ccttgatgct cacgagcggt cttcctcctt
    29581 tatggataag tgcaggcatg gggcactggg cgtcgggcca agggcgaaag gccaaggcct
    29641 gaggccgaat gtctgatgct catccgccta tatcccttcg gcgggcgaaa atcaaccggc
    29701 gatcttcttc cccgccatga tatcccggcg cttttcctcc gtcaggaggc atcccgtcag
    29761 gcagttcccc acgcaatctc cgtcgcaggg ttcggtatgg atcgtcacgc tcgccgatgg
    29821 gaaatgccat tcgatggcgc gggccagctc ctccgtgatt cggtgggatt cttggacgga
    29881 catgtcggga tccaccttca tgtggaattc cacgaatcgg aaatttcccg ccttgcgcgt
    29941 gcggagccga tggaagccgt ggatgatcgg ccggtgatcg atgatcagtt gacggatcca
    30001 ggcctcctct tccggcggca accggacgtc cagcaggtcc cgggccgact gaagcgtcaa
    30061 gtgccaggcc gcccggatga tcaggaacgc cacggcgatc gcgacggcgg gatcgagcca
    30121 gtggaggtcc aggtccgggg cgaaacgctg ccccagccag atcgcggcca gtcccgccat
    30181 gacgccggcg gaggtgtaga cgtccgtacg cagatgccag gcgtccgccg tgagggcgat
    30241 cgaatcggcg gcccggccga tccggaagag ccgggcggag acggcgtaat tggcggcggc
    30301 ggaaagcagc atcaccccga ttccccaggc gatgtaaccc aggggacggg ggtggagaat
    30361 gcgctcgacc gcctcataga tgatccatcc ggcggccagg aagatgagca gggcctccac
    30421 gacgcccgag atattctcca ccttgccgtg gccgaagggg tgccgcgtat ctgcgggaac
    30481 gctggaggtg cgtacggaaa acagggcgat gacggatgcg accaggtcca cggcggaatg
    30541 gatggcctcc gagaggatgg aaaccgcgcc gatggacagg cccacaatca gcttgaacag
    30601 gatcagaagg ctgttggagg cgatggaaag ccgggccgcc cggatcttct cttgctgcag
    30661 ggtcataagg gtccgcgttt cgttttacgc gtgcgatcga agccggtggg cggagaaggg
    30721 ccgcccgtga ccgggccaga tcgttcgggc gcctaggcgc ccgagggccg cccagctttg
    30781 cttcagcgcc gccggatcgt cggcgaaggg cggcatgatc gggccgccgc cgcagggcag
    30841 cacgttgaag gccaggtccc ccacgaaggc gttgccgtcg tcgagcagga ccgagagcga
    30901 tcccgccgta tgccccggag tcatcacgat ccgccccgcc attccgaagg gatccaggga
    30961 gagctcgccg tcgacgagga catccgcctc gaccgggtcg aaccggacga atccgggcag
    31021 cacgcgggac aggagccgac cggccccgat cagggcgcgc ccgaccggat gcacccccgc
    31081 cgggaagacc accgcccccc gccggagcca gtccgcttcc ccggccggaa cggcgacggg
    31141 acaacggcag gcgtcccgaa tctcgcggag gttgccgacg tggtcgtaat ggacatgggt
    31201 aatcacgatg agcgagatcc gcgcagggtc cataccctgt ttcgagaggg tcgtaaagaa
    31261 ttttctcccc tggcggggga atcccgcgtc aatcaggatg gatgcaggtc cttcgctgac
    31321 gatgtaggtg ttggcggtgc ccagggggac gggaaggatg cggggcataa accgtctcca
    31381 atcaggccgg atcagggatg ttccgcttcc gtgaggcccc cgggaaaccc ggccgaccgg
    31441 ttccaggagg cgtgccgggg aggggtcgaa aagggccggg cgcaccgggg gatcggaatt
    31501 ccccgggatc ctcccccgga tcaatagcgg tagacggcgg cgaggacctc ctggccgggg
    31561 atttttcccg gcggcaggat gtggacctcc cccccgttga tgaaggcctg gatcgcggcg
    31621 acgtcgagca ggtcctcgtc ccccggtttc atcgccgcgt gaagcgtcac ggcgccgctc
    31681 tccggggtaa agacgcccca ttgctgacgg ccggacgcga cgagcaggat gcctacgcgg
    31741 ccgtggacgg cggccgggac gatttcacgg atgtccgtgg ccgtcaatcc cgtgccggag
    31801 gactgccgga actgggcgat ggcgtcgtgt tccgcctttt cgtaataggg tttgaggatc
    31861 tgcaaggcct gtcggcggag ttcctcctct tccgcctctt ccgggttgcc ctggatgccc
    31921 tcgtccatca gatagggata ggtgttggca tcccggtaga tcgggaagag gtactcaacg
    31981 ccggccagca caaggggggc cttttcctcc ttcagaaggt cgcgcaggcc gcggtcgatg
    32041 aggcggaaaa actttaggat atcgtccttg acctggtccg cgccggcacc gtggccggaa
    32101 cgcgtggccg tctccgcgcc ggcccgcgac cggaagcgga cctggcgctc cggctcgtcg
    32161 tactgaagcg cctcctccag gctgcggggg caatgttcga gcggccgctc ccggaggccg
    32221 aagcggcttc cctcgaagag acgcacggcc ttctggctca gggccaggag gaagaagcgg
    32281 ttttcatcgc ccaggagcgg caggaggggc ttgacgtgga aacgctcacc cacgacgacg
    32341 agttccgcaa cgtcgagcgg cagggcgtac aggttcatga cgtcacggga gaggaacagg
    32401 gcgagtccat cccgccggtt tttccggaac agggcctggc cggtgatacc cgacaggggt
    32461 tccatgaacg ttttgacgtc cgggggcttg aggccgtgcg cggccagggt ctcttccgcc
    32521 tttcgcaaaa ggttgcggaa acgaatctgg ctctgctggg gtttggtgcc gacggcgctg
    32581 ggcggcatga agagcgacag gcagggtccc gtctgtttct gtatgagggt tttgagtgct
    32641 tccggggtta gggtatgcat ccgtccacct ccttcgtgtc atattcgaaa ggggatgttc
    32701 cgggccggaa ggaacgctgc ggcgggaaag gaagcggcct gtcgctggga cagccgtttc
    32761 ggcgcgcagt ccggttccga cacccatcag gccaaaacgg cctgtgccat gatgaatatc
    32821 ggatacggcc cgacaaagca aacgatttct ttgacaaggc ttttcaaatg gattatgacg
    32881 cgtcagatgg aaatccgctc cggcgagaac cgggatatcc gcatgcaaaa gttggggttt
    32941 gcccgcgggg gtcgtctttg aggagcgcca gagcaggggg aaggggtcgg catgtatacc
    33001 gcgagcgatt tgaggaaggg gctgcgaatc aaaatcgaca acgatccgta cgtcgttacg
    33061 gagttcaatt tcgtcaaacc gggcaagggc caggccctgt accggtgccg gttgcgcaac
    33121 atgatcaacg ggtcgcagtt tgagcggacg ttccggtccg tggacacctt cgagtccgcc
    33181 gaccttcagg aaaagaagat gcagtacctc tatcaggagg aggataaata ctgcttcatg
    33241 gacaacgaaa gctacgagca ggtgttcctc acccgcgaac aggtgggcga tgccgtgaac
    33301 tacctgatcg acaacatcga ggcccaggtc ctctttttcg aagaccgggc cctcgggctc
    33361 agcgttccca acttcgtgga cctcgtcgtc acccaggcgg atccgtgggc gaagggcgat
    33421 tcggtgacgg gcaatcagaa gccggtgacc ctccagacgg ggtatcagat cctcgtgccg
    33481 acgttcatcg aggaggggga gaaaatccgt gtagacaccc ggacggggga gtacctgacc
    33541 cgtgtcaagg gatgaaccgg atgcgggggg cgaatggcgc ctgacgagac ggcgggcggc
    33601 cctggagatt cgggcgcgga tgatccaggc cctccgccgt tttttcgtca cggcggggta
    33661 tctcgaagtg gaaacccccg tccggattcc cgcaccggca ccggaaaccc acatcgacgc
    33721 cccgcccgcc ggagactggt ttctccaggc ctcgccggaa ctttgcatga agcggctcct
    33781 ggcggcgggc tacgggaaga tcttccagat cgcgaagtgc taccgcgccg gggagcgggg
    33841 tgaccggcac cttcccgaat tcacgatgct ggaatggtac cgtccggcga gcgattaccg
    33901 cgacctgatg acggagtgcg aggtgctcct gccgtttgtc cttcaggccc tgggacggga
    33961 tgccgcgctg gtctaccggg gggagcggat cgacctgacg gcgccttggg agcgcctgag
    34021 cgtggcggag gccttccggc gcttcacgcc cctgaccccg gaagacgccc tccggaaggc
    34081 gtgtttcgac gagcggatgg ccctggacat agagccccgc ctgggccggg aacgccccac
    34141 cttcctttac gactaccccg cggcctgcgc cgcccttgcc cggaggaagg gcgcggaccc
    34201 cgccctcgcg gagcgtttcg agctctacat cgccggactc gaactggcaa acgccttttc
    34261 cgaactcacg gacgtcgccg agcagcggcg gcgcttcgag atggcccgga cggagcggga
    34321 acgccttggg aaggtggggt atccgtccgc ggaacccttc cttaaggcgc tcggtgcgat
    34381 gccttccgcc gccggcatcg ccctgggcgt ggaccgtctg gccatgctcc tggccgacgc
    34441 cgatcgcatc gacgacgtcg tcgcctttgt ccccgaagac ctgtagcctc ccctcccggc
    34501 tcattccacc ccggcgggga aacgccttga attcgggcgg gatcggaagg ttcaagtccg
    34561 ccgccaagcc ccttgacaag cgccggtttt tcctttacac ggggaacagc cgccgctttg
    34621 gcgcccgcgg gggcggctgt cttgagcgcg ccggggcgcg tttcgtttct gtggcgcacc
    34681 ggggaggcgc agggtcgaaa gggtccggcc cgcggaggga gctcgatttc tggggttgcg
    34741 gaggagaaag cagtgaatga aatttcgacg gttgcggcag gcatgggcgg tcagttccac
    34801 ggcagtctgc tcaacatgat cttggacgcc gggctgatgg ttcagctcgt tctcatcctc
    34861 ctgtttgtct tttccatcgt ctcctgggcg atcatctttc tcaagtaccg cacgatccgg
    34921 aaggtcaaac aggaaaacgg actgtttctc gacgcctata tgcagagcag caaactggcg
    34981 gaaatctttc ccgaggcgaa aaaatacgcg acgtccaccc tggcagcggt ctttcgcgcc
    35041 ggctacgggg agcttgtcaa gctgaccaag gcccggcggg agctggcggg aggacgggaa
    35101 gaggacgggg gcctcgccac cctggagctg cgggggatcg acaacgtcga gcgggccctg
    35161 aaccgggccg ccagcgagga atcctccaaa ctcgaaatgg cgctcggttt tctggccacc
    35221 accggcagcg cctcgccgtt catcggcctg ttcggcaccg tttggggcat catggacacc
    35281 ttcaagggga tcggtctgcg cgggtcggcc actttggccg tggtcgcccc ggggatttcg
    35341 gaggccctga ttgcgaccgc ggccgggctg gcggcggcca tccccgcggt catcttctac
    35401 aattactacc tccagcggat caaggcgatg acggcggaaa tggacaactt ttccggcgag
    35461 tttctgaaca tcatcgaacg ctaccagctg aagaaggggc aatgatggcg gcgagaagaa
    35521 gacgcccatc cctcgaagac cggccgatgg cggacatcaa tgtgacgccg ctcgtggacg
    35581 tcatgctggt gctcctcgtc atcttcatgg tgacggcccc catgatgcag atgggcatcg
    35641 atgtgaacct gccacgggtg caatccaaaa gcatcgatgt ggccgaggag aagattgtgc
    35701 tgtccgtcaa tgcccagcgg gaactcttcc tcaacaggat tcgggttccg ctgggtgagc
    35761 tccgcccgaa gctcgaggcg atttttgccg cgcgcatcga gcgggaggtc tttttgcggg
    35821 ccgatcgaac cgttccctac gggttcgtgg tcgaggtgat gtccgaggtg cgccgggcgg
    35881 gtatcgacaa actgggcatg atcaccgaag cccccgagga gcggggatga tgtccgcgtc
    35941 ccctctcacg gagaatccgg gcgggggggg gtccctttct gggatgcttt ggctgtccct
    36001 gatcctgcac ctggcgttgg tctccgtcat tctttccctg ccccccctcc ctcaggcaaa
    36061 gctgaccctg gggccggtct attccgtgga actggtgagc gccccgccgg ggctccctgc
    36121 accgtcatcc gatctttccc ccgacatcct gaacccggaa gcgggccgac ccgccgcggt
    36181 cctgaagaac atgaaggcat cgacccttcc cgagcgccgg accgaggcgc tgagaaaacg
    36241 tccggaggcg gcggaagccg ccgtcgaggc gattcgcaga aaaatggcgg cccagccgcc
    36301 ggccgttccg gcgccccggg tgacggcgga gacaacggct ccgacggttc cggcggaggg
    36361 gagggccagc ggggcggaga gacaggcgca ggcgcggcga tacgcggcgc tggtttccca
    36421 actcgtctgg agccaatggg cctttccccg ggacagtctg ctgcccgtgg gcagcgaggc
    36481 cgtggtgcac gtgacggtgc tggcaaacgg aacggttgcc cggatgggtt ttgagaaacg
    36541 ttcgggaaac cgctacttcg acgagtcggt cctgaaggcc atccggaagg ccagcccctt
    36601 tcccgcgctg cccgccgaaa tcggcggcgg acgcctggag gtcggcatcc gctttcaccc
    36661 ctcgcgcggg caatgaatga aggacggaga aacggtgatt ttcagaggct tctgcgtgct
    36721 tcttctgggt ctggggatct ttctgggcgg ggccacaacg ccggggtggg cggtggaagg
    36781 ggcggagcgc gtttacatcg acatcgattc ccctcacttc caaaagttcc ccctcgcggt
    36841 gacccccttt tacgcgctcg gcccgtcccc ggagggggcg gagctttccc gctggttttc
    36901 cgcgtctctg acggagatgc tcgcaattac gaactttttc caactgatcc cgccgacggc
    36961 ctttctggaa gatccggtcc atgccgacag cacgtttcag ggaacccact ttcccgactg
    37021 gcgcgccatc ggggcggagt acctcgtcaa gggcggctac cgggttcgag ggcaggagtt
    37081 gacggcggaa ttccggctct tcgatgtcgt ccggggggag gtggtccgtg ataaacgata
    37141 cgccggaccg ctggctgaga gaaaggccat ggtgcagaag ttcgccgggg aaatcctttt
    37201 cgcgctcacc ggcgacgggg gcgtgttcaa cacgaaaatc gccttcgtct tgaaacccgg
    37261 gcgggagggg gagattcaca cagtcaactt cgacggttcg gatctgatgc gcttcacccg
    37321 gtggaagacc ctgacccttt ctccgcgctg gtcgcccggg ggacgctacc tgacctatac
    37381 ctcctaccgt gacggtaatc cggaccttta cgtgcagggg tatccggggg gcggaacgaa
    37441 gaagatcgcc gcgtttccgg gcctgaacct ggccggaccc tggtcggcgg acggccagcg
    37501 aatgctgctc accctgagcc gggatggcaa tcatgagatc tatgtcatgt ccctggagga
    37561 cggtcggctc gagcgcttga cccatagcgc cgccatcgac gtctctccaa ccttgtcgcc
    37621 ggacgggcgg agcatcgcgt ttgtgtcgaa ccgctcggga tcgccccaga tttacgtcat
    37681 gaaccgcgaa gggggggaag tgcgccgcct cacgtacgag ggcggctaca acacctcccc
    37741 gtcctggtct ccgaaggggg atcggatcgc ctttgaagcg cgggtgggcg gctcgttcca
    37801 gatccacacg atccgtgtgg atgggggcga ccagacgcct ctgacgacgg acggcgggga
    37861 tcacgagtcg ccgtcctggt cgccggacgg acgctacatc gcgttcagca cccgcggggc
    37921 aggggtgagt cagatctgcg tcatgaacgc caacgggacg gggaaacggg ttctggtgga
    37981 ggcgccgggt tgcctggccc ccgcctggtc cccgcggctg ccctgagcgg cgccaaaggg
    38041 cgtttttctg gttgacctgt gtcggcggag tatgattaaa ggagcatgtg gtgtcgcgtt
    38101 cccgggcaga tgcggaaaaa ggcccggaat attttgttgc ggcggcgttt tttctccatt
    38161 tttcgagaaa ggagtgtgcg taatgattca caatcggacg aggatgaagg tgctggtctg
    38221 tctattctgt ttcgcgatga tggttgtttt ggggtgtgcg acgacatcgt cccagaaaga
    38281 ggtcttggtg gaaggggagg atgcggttgc cgccgagcag acgggggctg ccgcggaagc
    38341 ggtcaaaccc ggggaaccgg aaccgattat cgacgagacc ctggcggagc ccgatgccgc
    38401 cgcgcctcag atggccgcgg acgaggtgcc cccggttccg gtggcaaaac cggcctcaac
    38461 ggtttccgct gcgagcgagg cgcctgcctg ggagaacatc caattcgatt acgacaagtt
    38521 cagcctgaac ccggaagcga gggtcctgct cgacaaactg ggcgagtggc tgctcaggaa
    38581 taagggctat gccgttgtca tcgaggggca ctgcgacgag cggggtacca cggagtacaa
    38641 cctggcgctg ggtgagcgga gggccatgga ggcgaaggcc tatctgaccg ggctgggtgt
    38701 ggcggccgcg cggatcaaga ccatcagtta cggaaaagag atgcccctcg atccgggcca
    38761 cgatgaggag gcctgggcga agaaccggcg ggaccagttc ctggttgagc ttccccgtta
    38821 agcgcgtgag ggcgttttaa gtgagaaaac cgttatctct cgtttcgctg gcggtctttt
    38881 tgatcctggt gggctgcgcc agccagcagg atctgaagcg cgttcgtggc gaactcgatc
    38941 agagaatcac cgccgtggat cagagggcgg cgcgccttga gcagcaagac ctggagcgcc
    39001 aggaggcgat ggccgccctc atgccggagg tcggcaagaa ccgggaggac atcgcggccg
    39061 tccgtaaggc tcaggcctcc cagggggcgg acatgacgga gttgcgggat caggtacagc
    39121 aactccgggg aacggtagag gggctccaga aggaggcggc cgtcctttcc ggccgggccg
    39181 gccgaaagga cgaagaggtg aaggacctcc gtgagcggct ggacgccgcg gcgttcaagg
    39241 tgaatttcat cgagaactac ctcggcatcg gcaagaagga agtccggtcg gagaacggcg
    39301 ataagtccgg aaagtccgtg cgcgagagcc tcaagggtaa gacggacaag gattccgcgt
    39361 atgccatggc ctacgaaacc ttcaaggagg gccgttacga aaaggcacgg gaggagttcc
    39421 agaactttct caaggcccat cccgcgacgg agtattcgga caacgcccag ttctggatcg
    39481 gtgagtgcta ttatctggaa cgaaaatacg aaaaggccat tctggaatac gaaaaggtgg
    39541 tcaaggggta tcccgagggt gaccgggcgc cccatgccct tctgaaacag ggactctgct
    39601 tccagagtct gggcgaccat tcatccgctc gtctcatcct gcaacaggtc atccgggcgt
    39661 atcccaacac gagttcggcc cggatcgccc gggcgaagct catggagatc aaataagcgc
    39721 aagtcccagc cgctgagatc gcacggcccc cctgtggggc cgtttttcgt ggtggccgca
    39781 aagccggctc gacgggcgtc gtcccaggga ccgcgcgccg ggccggcgcc tcagggagac
    39841 ccctccggaa gccttcgggg cagggttccc ggcgggatgg ggagatcgaa tcgctccggg
    39901 acggtgtccg gggaaagaac cgggtcggtg taacggaccg acatcttggt cgcgggcggg
    39961 ccgggggcgg tgaaacggat ccgcatcggc cagggacgtc cggaaaggag gcggtaatcc
    40021 tcgaaccgga ccgtgtagag ttccttgccg tcctcagcga aacgggtgca gcgctgcacg
    40081 tggcgggatg ccgggtcggt ccacagggtc aggcgtttcc cgtatggatc gaacagatcg
    40141 agacgaccag cgtccgtgtc cgcctccggg accgtccgca cggaggtgat gccttcaccc
    40201 ggtggacagc tcccccgcag gagtgcgatc agctccccgc cggacaggcc gatcggcaag
    40261 aaggaggaca gattttcccg gttcggtgcg cccgtccagt aggccttttc ccccgggacc
    40321 gagatctgca gacggcttcc cgccaaggtc atgaagaagt cgggcggtcc caggagggga
    40381 aggtgctcga tgcgcaaggc gtcgggccac ttcatgagga tcgcggcctt caggggataa
    40441 gcgccccggt ctccggagag ttcgatgcgg gtcagggcct gaaaggtgcc gtcgaagcgg
    40501 tcggccgagg cgagcgccgc cagggtgtgt tcggggatgg tcccgaccgg ggtcggccgg
    40561 agggcgccgg tcggcggcct gcatcccgcg atgcccagca tcaggacaac gagcaggagg
    40621 agggcggttt tgttgagcgg ggcagggctt tggggaacgg tagtgtgcat gcgtgaagat
    40681 tattttctct tttgcagatc ctggagcttc ttcttcaggg acgcgctttc cggggtcagt
    40741 tcgagcgccc gggcgtaggc ttccgaggcc tcccgggaac gccccgcctt ggcgtatgcg
    40801 tcgcccagat gctcggcgat ggtggcatct ccgggcagga gcccggaggc ctccttcaga
    40861 tatttgacgg ccaggtccag cttgttctga cggaaataga gccaccccag actgtcgatg
    40921 atgaagccgc tccccgggga gatcttcagc gcccggcgga tcatgcgttc cgcctccttg
    40981 agatggatgc cccggtcggc gtagctgtaa ccgatgaagt tgagggcttc ggcatggttc
    41041 ggatcgattt tgagaacctg ttccatggcc tggatggccg cttcgaagcg gttcgtcttt
    41101 tcgtacagaa cgccgcggcg gtaatggaga tccgtgcttt ccggagagaa ccggagcccc
    41161 tccgccaggc agtcctccgc cgcctggagg ttcttctgct cctcgtggag ctgtgacagg
    41221 tacaggtata gggagggaag cgcgacgttc agggtcaggg cttcccgaat ggaggcgacg
    41281 gccgcgtccg gttgcccctg ccggttcagg atcatgccga tccggatgcg ggcgttcccg
    41341 aaataatcgg agctcaccgg cactcgccgg tattcctcca gggctttggg gaagtccgcc
    41401 ttttcttcgt aggcggaggc gagcaggtag gtcagcttcg cgtcttcggg gcgctccaga
    41461 agcagcgcca ggagctgttc gatggcctga tcgggccggc ccttttccag atagacgatc
    41521 cctagggcca ggcgggcctc ctggtgcgaa cggtcttggc tgaggattgt cgcgaaggcc
    41581 tgctccgcct ctccgaagtt ctgctcacgc agataaagct gtcccagtcg cagccgcacc
    41641 gtgatccggt tcggatacgt cgccagaaaa tcgcgataga gttgaatggc gagatcggtg
    41701 cgccgctggc gttcgtaaag gctgcccaga tccagcagaa ccgcctccca caggggtttc
    41761 agggccagcg tttttctgaa ccccgcctcc gcctccgtga gaaggcccat gtccttcagc
    41821 actcgggcca ggtaatagtt gcccatgggg tgttcggggt tgatctcgag aagcttccgg
    41881 aagtgttccg ccgccttgtc gtaccgatga ctgtccgcat agatcgtccc caggtagagg
    41941 tgcgcggatt cgttgacggg atcgagctcg atgacccggc gataccggcg cagggcgttc
    42001 ttcgtgtcct gggcgttcag gtacaggccg ccggagagga gatggacgtc gatgtcgtcg
    42061 ggatggcgga ggagggtttt atcgcacagg gcgatcgctt tggcgggatt tcccttctcc
    42121 agataaagag gggccagttc gagggtgatg gcgggggcgt agggatcgta tcccatggcc
    42181 gtctccagtt cccggatggc ctcatcggtc ttcccgtcca gggccaggaa aatgccgacc
    42241 gaatagtggt aggccgcctc ccgcgaggcc ttgtcctcgg acggttccgc gaccggcggc
    42301 gccggcggcg tcaggggggc gcacccgctg aacgcgccca acagcaagag gatcagtagg
    42361 ccttttctca gaagatcaaa attcattcgg gccggttttc tcatcggcgg aggggctccc
    42421 cctccaggag cagggaggaa agatggacgg tttccgtccc ctccaccttg agaaaggtca
    42481 gggcttcccg cagggcggca agggtttccg ggtagggatg accgatcatc accagaacgt
    42541 ttcgggcgcc gggcgggccc ttccgtacgg cctgcttgag gatgtcgagc gtagcgcccg
    42601 catcgcggcc gttgtcgaga aagatgtccc gggcggcgaa tgggaccccc gcaagaacgg
    42661 ccaaggcctc cacgcgggaa ttggccgtcg tccggctgtc cacgaagtac aggtttcgct
    42721 ttccgatgac cgccagcgcc gcggacacct tcccctcttc cgccatgaag gcggaaccca
    42781 tgtggttgtt gacgccccgg atatgcggga cggcatccag ggcgcgctcc acgcggcggg
    42841 cgatttccgc atcgttcaac gccactagaa gtgcgccttc ccccggatcc tccccggggt
    42901 aaccgcgggg ttccatgggg aggtggagga gaacctcctg cccggcgtcg tgaatcatct
    42961 ccgcggcctc ccgggaatgg cgtctgtagg ggaggacggc gaagttcagg gggattccca
    43021 gccggagcag ctcacgcagg gggcgcaggt catgaccgat atcgtcgatg aggatggcga
    43081 ccttccaggc cggggcccgg ggggatcggg gacgtgcgtc cggtgggcgc cgctccgcat
    43141 ccggcggaag gaggtccacc ggggagctct ttcccctacc ggtaggggtt ccgtccgggg
    43201 tcggtagccg catcgcctcc cagtggcgcg tcaacgggat gaacaaggcc cccgccagga
    43261 gggcgagaaa gagaaccacg acccacccgg tgcgtttttt tttcttcttt tttcccatgg
    43321 attcctgagg ggcgccaacc ttcgggccgt cctcaactcc gcatgttttt cctgaagacg
    43381 tcccagctct tcagaatatc gatggccgcc ttgagctgat tgtccaggtc cgtttccttg
    43441 cggagctctt ccgccggcgg gccgttttcc ttctggggcc ggatatggcc gcggagatcc
    43501 ctctcccgca acggttcatc cgcctgatcg ttctcctcgg cgggccggat gaatttcacg
    43561 atcacgtcgg gcgcgatgcc ctccgcctgg atcgatcggc ctttgggggt atagtacttg
    43621 gccgttgtga gcttcacggc ggatccgtct tccaggggga tgaccgtctg aaccgaacct
    43681 ttcccgaagg tctgggtgcc gagaatcagg gcgcggccgt tgtcctggag ggcgccggag
    43741 acgatctccg cggcgctcgc cgttccctcg ttgacgagaa tgatcattgg acaggccggc
    43801 tcgtcgccgt cgtctctcgc catggccttg gtttccatgc tcttcgcccg cccccgcgtg
    43861 gagacgatga ccccggattt gatgaagatg tcggagacct cgacggcctg gttcagaagc
    43921 ccgccgggat tgttccgcat atcgaggatc aggccccgca ggagtttccc cccgtcggtg
    43981 acttcccgca gggctttctt cagatcctcc ccggtccttt cctggaactg aaggacgcgg
    44041 atgtaggcga tatcgggatc gagggccttc gtcttgacgc tcttgacgtt gatgatggcg
    44101 cgggtgacga ccacgtcctt gggcttggtc aggccttccc gcacgatggt cagggttact
    44161 ttggtgtcct tggggccccg taatttcttc accgcttcga ggatagtgat gtctttggtc
    44221 gactggccgt cgatcttgac gatctggtcg cccggcttga tgcccgcctg atgggccggg
    44281 gtgtcctcga tgggtgcgac gaccgtgagg atgtctttaa ggagggtgat ctcgataccg
    44341 aggccgccga aactaccccg cgtctccacc tcgagttcct tgtacatctc cggggtcatg
    44401 taggcgctgt gggggtcgag ggatttcatc atgccatgga tggcgccctg gatgagggtt
    44461 tttgcctcga tgtcctccac gtagttcttt tccaccatgt ccaggacttc gttgaaggtc
    44521 ttcaggtgtt tgtacgtgcc gcgatcgagc gcggaaacct gatggtcgcc gtaggggccg
    44581 aggatcaaga agagtgcgga tacgcctgcc aaaatgagac gacgcttttg catcggcgtt
    44641 ttcatgccat aacctcctcc ggggtcgaga cgggagagga acggaacggc ccgttcatcc
    44701 catcctctgt tagcatgaac ttcatgtaaa attccacctc tattttcttg cggttgcgtt
    44761 ggcaagtgga gggtgacgag gcgtttcgtg gagttgtcgt cgcgcaacat atggacggtt
    44821 tgaaaacgat atgttgtgcg tactgccggt ccacgcgtgg gggacaactg gctaaagatt
    44881 cgaaatagcg cgtcattctc agcaaagacc cttggcattc attttgcaaa ttcagggaaa
    44941 gcaactcgct catgaaaccc gtcgcgcaag acgaagggcg aggcgttcgt gtggattcat
    45001 ccaattcaac gacagcgtaa ggagagcgat catgacagaa aagtacgacg tggtggttat
    45061 cggggccggc gtggccggat tggggacggc ggccatcctc gcccgggatt tcaagcagaa
    45121 ggtgctggtc ctggagagaa atccgttcat cggcggacgg ctcgcgtcct tcgtcggcaa
    45181 gggagacaag gtctgcatcg acgggatgga actggacgcc aacgggttca agcgcgttct
    45241 ggggatggtc ggcgcctggg ttcccaagtg cacgccggac ctggaaacct gtttcaagga
    45301 gaagatcttc gacggatgca ccctcgatac cggccacggg ttgttctggg gcaacaaggg
    45361 caagatccgc atgcttatga actacctcga aaagcccgtg gacatgccct gcaacgtcgg
    45421 cttcgccttc gtcgactaca agaaggggaa caaggcctat caggtcggca agggcgaacc
    45481 ctacgcctgg atgagcaagg agggcttccg ggccaccatg atggctctcc gggacatggc
    45541 actgatgagc ctggccgata tcgcacagac cgcgcacatg tcgttcgagc agtggctcca
    45601 ggcccgaaac gttcccaagg aggcctacga ctacatcaag gtcctggcgg cctcgcagac
    45661 gggccaggcc gatctcaacc tgaccccggc gccggacgtc ctggggcaca tggccacggc
    45721 cggtccgatc cggatgaacc tcgtcgaggg ctcctgcgcg accgtcgcca atcccggcac
    45781 catggccttc gcccttctga tggagcaggc gctgacggcg cacggcggga aggttctgcg
    45841 cagcgcgccc gtcgtggagg tgatcatcga aaacggcgtc gtcaaggggg ttacgttcca
    45901 gtacgagaag ggcatcgaga cggcctacgc ggaccgggtc atctgtaccg ttccctccaa
    45961 acagatgctg aacgtcatcc atccccggta tttcccccaa gagatcgtcg atcgggtcca
    46021 gaccaaattc tggggcgccg gcatgatcac ggggtacgga aacatgaagt ccgacatctg
    46081 ggcggacaag gggatcgagg agcggagctt catgtacatg ccggatgtga ttggggcgga
    46141 ggagggctac agcggctgta tcgacatggt gttgtggaac ctggcttcgg tggcgcgggg
    46201 gagcaactcc aacccttccc tgacgagtga gaccggcgcg gcgccggagg gcaagcatgg
    46261 ctggatcttc tcgaccgcca tgacccacga ggagatgagg acgccccgga aggttgcgcg
    46321 cgtcgtggaa tggaacgaag actggtggaa aaagaccttc ggccgggcca agtgggaggc
    46381 cgagatggag ttcctgctct ggatgtgcac ggatcatgcc tttggctgga tccagcccat
    46441 cgggaccgac cggatcgacg tcaagtcccc ctatgtcgac gggctgtact ttgcgggcga
    46501 ccagtacggc gagcgcctgt ggggctgtgg ggtggacgcc gccgcgctga gcgcgacgct
    46561 gtgcgtggac agcatgatgg ggtccaacgc cgaggagaag gttttcccct tctaccaccg
    46621 ggcggtgccg ccgtggaaga aggcctggta gacgtgtccg gtctggcaga ggccgcattc
    46681 gaaaatgaaa aggggctgca ggcgatttgc ctgcagcccc ttttttctgg ggtgtatcgc
    46741 aacattttgc gggtggaacg attcccgatt tgtgcccggc ttggacgata cttccatcgg
    46801 cgctacggca ggccatctgt tgaacgccgc ggggaaggtg aggccgcctc aacgaacggg
    46861 ccaggatacc tcacccagcg ccatgcggca aagatcggtg atgaagatct ggggcaatcc
    46921 ggccttagcc gtctgatcac gcaacaccgt atcgcacatt gggcagatcg tgatcatggc
    46981 ctcggcatgg ttgtccatcg catcccgaat gttgttcttc tgaatgccca tggagagttc
    47041 gttgttgacg aagagtgccg gtcctccgca gcacagggca ttcacccctt catacgttcg
    47101 tgaaacgcgc tccacaccga tcagttcaaa aatttcattc agccagatgt cctgttcggg
    47161 aaaccacctg accgcgcagt tcggctggta cgcgaccatt ttctttaatt ttttgatgtc
    47221 tctttggttc tccttgaaat agtccaacag atactcgaac aggtgcaaat atttgtaggg
    47281 cacctcgatc ccgagctccc tggctttgat gtgagccaga atatatccct cgttatgcat
    47341 gtaaacgatc tctttgccca gctcagccaa cgaatcgatc accctccgac catatttttc
    47401 ggcaaggctc tcccctccca tatgggccat accgaccagc gaggcgtact ttctcccccg
    47461 aaccaccgtc atgcccttga acatccggct ttcgagcgtt ccggccggga actgcgcgaa
    47521 ggtaaacgaa tcgaaggaca gcgcaggttt atcggggtct ccttcaatca cctgaatatc
    47581 ccggctgcct tgttcgaagg tcttgaccac ggaattgatc accggtttga agcccacggt
    47641 gatcgggctc cccatcttct cctgcatccg gcagatcagg ttcgccggat ccgccccggt
    47701 cgggcaatac tgaaaacagg cattgcaggt gatgcaatgg tccaggatgt ccgcggcttt
    47761 tccctccatc agaagcttca tttcactcgc ggccctgtcc ttgtcgtaat cgacatatcg
    47821 gcacttgacg aggcaatcac cgcacaaatc gcacttcaaa gcgtcccaca tcgttcacct
    47881 ccatgaaagt aggttggcag atcggcctgc ctccgggatg ataaaatgat ccgttgtcgc
    47941 ctcataaaga tcgattctcc cgcgttcgtt cattccggag aaccgatccc ggcgctttct
    48001 aaaaattaag gatacgggac gcctcatctt catcgacatc cataatatac tgaatgccgc
    48061 tgacgcgtgc cgcctcgggt gtcaggctgg cgatatcgtt gcgcgacatg tactccaggg
    48121 aaaacttccg gctgccggcc atcaactggc ggagcccctg ggccaatctt tcataatacg
    48181 tgtacagtcc gatggcgccg gtgggcagct tgtcgaattc tttgtcaccc agctccttgc
    48241 ggagcgtgct ggcggtcacg aatatttcat cttttgtgtt gccgaagcgc tcaatataga
    48301 ccggcacctg ctgagcctca atggtctggc cgatggtctt tccgaccatg gccgccgcga
    48361 tgggcgcccg ggccatgccc accatcttga cgtagggcgc gcccatggcg agggccttga
    48421 atatctgatc ttcgaatgtg aaaccgccgt tgacggaaat ggccggcaga tatttccccc
    48481 ggtccgccag ctgtttggcg tattgataga cgagggtatg caattccacc ggcggaacgc
    48541 cccactcgtt catcattcgc catgggctca tgcccgtgcc gccgcccgcg gcatccacgg
    48601 tcagcagatc cagtttgtat ttcgacgcga aggcgatcgc cctggccagg tcaacgggcc
    48661 ggtaagcacc ggtcttgaga aaaatgtatt tggccccggc ctcgcgcagc ccttcgatag
    48721 tctcggcaaa tccctcttcc gtgaccatgc cgacccgcga atgccgctcg aactccttga
    48781 acgccccctt ttgaaaggct gcgatgacgg tgggatcgtg gggattgggc aggacgatat
    48841 agccgcggtc gtagagcatc tgggccttct tgaggtcctt gattttcact tcgccgccga
    48901 tgtccttcgc gccctggccc catttgagct cgacgcactg aaccccgagc ttgtcgatgg
    48961 cgtactgctg caccttcagg cggctgtctt ccacattggc ctggacgatg atggccccgt
    49021 atccgtctcg ctggttatcc ttgtaaagtt tgacgcggcg cttcaaatcg acggtgtcca
    49081 caattttgcc gcccttaaat ttggtttcag ggtccatgcc gaccacgttt tccccgatgg
    49141 tcagacccgt ccccgccaag gcggagccga tggccagtcc ctcccagttg tttttggcga
    49201 tgttggtgga accgatgcct gaaatgagcc aggggagctt gaatttgatg cctttatcat
    49261 ggccgatgtt cacctcgaga ttgacggccg ggaagatggc cttgtcgctg tcggcttcga
    49321 tgccgaaggc gccgctggcc gtgcccataa tgttgaaatg ggaataatcc acggggaatt
    49381 tcttctcggc ggcggtggtg atgacgccga aaggctgggg atagatgacc tcatgcccgc
    49441 ggtaggcgga tttgccgatt tcgcacatcc cgatgcaccc gtcgatacac gtgacgcaca
    49501 tgcccgatgt cggcgtgatg gactcctcgg tcctgttttt agtaagggtg gcgcttgagg
    49561 tattgattct tgaaaaagac atgtatcctg ctcctttctg gatttggtca ttttttggct
    49621 tcacacgcca cacagggcga catgtaacac atcatgtcat ggaactgttc cttctcgatg
    49681 cagggcgggc tcaagttggc gcacccgccg cagacggcct tttccggttc gttgtaggtg
    49741 cagcgggcct gacaggccgg gcagacctcg atgcacccgc cgcacaaccg gcagacgtcg
    49801 gaacggatat cgaagggggt gccgatgctc cggttctgcc cgcggccgcg gaacccgatg
    49861 gccccggcca tcatctgctc cttgcacatc cgcacgcaca gtccgcaaag gatgcagtcc
    49921 tcgtgttcct gccggaaccg ctgctgccgg acattgtgcc gggatgcgat gtcctggatg
    49981 gtcttggact gcggacagga cgccagcaga agctcgatga tcattctgcg cgccctcaag
    50041 acgcgttcgg aggcggttcg caccatcatg ccctcttcaa cagggtacgt gcaggatgag
    50101 acaagtttgg cgcccggccc cttgccgatc tccaccacgc agagtctgca ggccccgtag
    50161 ggtgtcagcc cctccttatg gcagagcgtc gggatgggga agccgtaaaa cctggccgcc
    50221 tccaggatgg tggtgccctt ctcaacggat atggaaagcc cgttcagggt caggttaatc
    50281 atgctttgct ccttcaatcg tatgagatgc cgcttgactt gcggatttgt cgcgggtgaa
    50341 ggccagatcg cagcgcaggc agcgcctgca ttccgcgcgg gccttatctt ccggcaacac
    50401 cagagtcacc tcccggaagt tctttacccg ttcgtgaggc gccaggacgg cgggctccgc
    50461 gcgcctcgcc gtttgggcct ccgtttcgtc gatgacggcg ggttcgatat aggtgctggg
    50521 cagcttcggc gccgccgctt tctcgatttt tcccttctgc aggaaacggt cgatgctctt
    50581 agcggccttc ttgcccgccg cgatggcgtc gatgacggtg ttgggtcccg ttaccaggtc
    50641 gccgcccgcg aagacccctt tgaggctggt ttcgagggtt tttctgtcga ccttcaatct
    50701 gccgccccga ttaacgtcga ggcccatcga ggcgagaaat tcactcttgg gtttttcccc
    50761 gatggccacg atgagcgtgt ccagttccac cctgaattcg ctgccgggga tgggaacggg
    50821 ccgtcgcctt ccggtggcat ccacctcgcc cagccggttt ttaatgcatt caatgccgat
    50881 aatccggcct tccttcgaga agatcttaat gggcgtgacg agggtttcca gctccacgcc
    50941 ttccttgagg gccgcttcga tatcctcctg gcgcacatgg atcttgaccg gcgagatgag
    51001 cgtctcaacc ttgacgccct cctcgagggc cgcgtcgatc tcctcttcat aggccggcat
    51061 ctcttcgcgg gtccgccggt agaaaaggga cacgctggtg acgtcctttt gccgcagggc
    51121 gacccgcgcg gcgtccacgg ccgaattccc tccgccgatg atgcccacat gcccccgcgc
    51181 cagttcttcg ccccggatgt tgaacgcctt caggaactgg atggacggga agatcccctc
    51241 gacatcctcg ttctccaggt ccaggcgcca gctctcgtgg gccccgatgg acaggaagac
    51301 ggcgtcaaac cggtcgttca ggaggtcctg caaagtcatg tctttgccca gggcgcgtcc
    51361 atactcgatc ttgatgttcg gattgaggag ggtcttgatt tccttctcca cgatatcacg
    51421 gggcagccgg taggccggaa gcgagcaggt cagcatgccg ccgggctgat tttccgcctc
    51481 atagatggtg accccgtagc cgatcagcga caggccatgg gccgccgtaa gccccgccgg
    51541 gccggcgccg atgaccgcca cctccttttt ctttttccgg gcccgttggg gcttgtagac
    51601 ggaaggattg acacggtcgg tgacgaaccg cttgatggcg cggatggcga tggcctctcc
    51661 gccgctcaca ccgaggctgc agcggttttc gcatttgcgg tcgcagaccc gggcgctgac
    51721 ggcgggaaac ggatttgccg tacggatcac gcggtaggcc tcttcgtaat cgcctttttc
    51781 caccatggcg acataccgcc atggttccgt gtccacggga caggctgcct ggcagggcgc
    51841 ccccgtcagt tgtttgcaga caaaggcccc gcatttttta tcttcgatgt gccgctcata
    51901 ctcctcgcgg aaataccgca gggtgctcat gaccggattg gcggccgtct ggccgaggcc
    51961 gcacatggtg gtgtccttaa cggtcagggc cagctcctca agtaggacga ggtgctccgg
    52021 cgtaccccgg ccctccgtga tgtcctcgag gatttcatgc atccgctgcg ttcccttccg
    52081 gcaggtgaag cacttgccgc aggattcgtc cttcaggaag ctcataaaat acttggccac
    52141 gtccaccatg caggtgttct cgtccatgac gatcatgccg cccgatccca tgatggaccc
    52201 ggccttcgcc aggctgtcgt aatcgatggg cagatcgaac atggaagcgg ggatgcaccc
    52261 gccggacggt cctccggtct ggatggcctt gatccgtgcc gcgctgacgg ggccgccgcc
    52321 gatatcatag acgaccttgt tgatgggggt cccgaggggc acctcgacaa gccccgtatt
    52381 tttgatcttt ccgacgaggg agaagatctt cgtccccgcg ttgttttcaa cgccgatctt
    52441 cgtgtaggct tcagcgccgt ggttgatgat gtagggcacg ttcgccaggg tctcgacgtt
    52501 gttgatgcag gtcggatggc cgaaaaggcc cttctcgatg ggatagggcg ggcgctggcg
    52561 aggctctccc attttccctt cgatggattt gatgagggcc gtttcctccc cgcagacgaa
    52621 ggcgccggcc ccgtgaaaga cctctatttc aaaatcgacg ccccggccca aaatgttctt
    52681 ccccaaaagg cccagacgac gggcctcccg gatggcgatc gtcgcgtgct tgatggccag
    52741 gggatattcc gtgcggacat agatgatgcc gtcgcttgcg ccgatggcga gggcggcgat
    52801 gatcatccct tcgatgatag cgtggggatt gccttccagc atgctccggt ccatgtaggc
    52861 gccggggtct ccctcgtcgg cgttgcagat gatatatttc gggccgccgc cgtttttcat
    52921 gttccgcgcc atctcccatt ttcttccggt catgaatccg gcgccgccgc gtccccgcag
    52981 cttggatttc aggatttcat cgacaagcca tgcccggtcc tgtttttcca gggccttttc
    53041 cagggccgcg tacccgccca ttttcaggta gtcgccgatg cggatgggat cgacatgctg
    53101 gttctgcccc aggacgatcc gggtctgccc ctggaaaaag gggatctcct gttccgaata
    53161 gcgtttgacc gtggagccgg gctcccggta tagaagctcc tcgaccacct cgcccttcac
    53221 ggccgcatcg ataattttgg gcacgttttc catcttcaat ttcggataga ggttgtatcc
    53281 cggctcgacg atgatgaacg gatccatctc acagaagccc aggcagcccg tcacccggag
    53341 ggagagcttg tcgtgcaggt tctgctccag aatccggcgt ttgatgatcc ggatcagatc
    53401 attcgagccg ctggcctggc cgcccgttcc cccgcagatg acgatgcagg gttttttctc
    53461 ttcgtttttg tcagtcagaa atgatgcgcg atacgcgttg aatgcagcga cggtttgaaa
    53521 agtcttcatc ttgttccctt tatcggttta tgacagattg atttgattca gattcaggag
    53581 gtgtccccgg cacccccggc gtgtgcagat ctcccaggcg cagcccgccc ggacaagcag
    53641 ggcggccata ggggagccgc attccggaca gatggaaaag ctcggcagtt cctcgtcaca
    53701 gtggggacag aataacggcg atacctgatt gagcgggaca ggatcttcca tggcgacagc
    53761 cccgctgccg tagagggaag acaggcgaag cgggtttttt ctgccgttcg cggagacgtc
    53821 gatcctgacg gacggatgac cgtcgagggg atggtcgggg tccatgaggc tcgccttgca
    53881 taaggggcag gataaaggca ggcggaagat ccggtcgtcc gtttccgtgt tgatcttttc
    53941 catctcttcc ttcgtcctgg caatgatttc tctcacctcg cccttggtga cgtgcggaaa
    54001 ataatgtccg tccgccacga ccgtcggtcc cagggcgcag gccccgagac agttgaccgt
    54061 ctccaggctg atctcgttat cgggggttgt cccgcccggg gcgatcttaa gctcatactt
    54121 gaattcatct acgatggctt gggcgccgcg gacatggcaa gccgtgccga ggcagacgga
    54181 gatgcagtgt ttgccccggg gcttcaggct gaaggccttg tagaacgtgg ccaccccgta
    54241 gatgtccgtc agcgaatgcc ccgtttcctc ggcaacgagc ctcaacgccg cttcgggaag
    54301 ataggtatag gttgcctgga tctcttcgag gatggcaata atcgctcccc tgtttttgtc
    54361 gctccttttg atgatctcaa ggatattttc cgtgtccatg ccactccttt ccactacgga
    54421 acgtacttcc ggctgcttga gcggatcgcg caaccggtat tcctcgcaat gcattggatt
    54481 gattgataag acattgcttc tggcggcgtg gagtataggg gggctgtttt tttgtgtcaa
    54541 gtgataatat tgggtaattg atcctattct gccgacctta tcacaggtgc cagaacagga
    54601 tcaattacca aatcttttac aaatctttta caacaaaaaa aagggttcca gaatatctct
    54661 ggaacccttt actgatggtg ggtcagggca gaattgaact gccgacactc ggattttcag
    54721 tccgatgct
//
LOCUS       DAOOLJ010000011        51791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_458_length_51791_cov_14.407975, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000011 DAOOLJ010000000
VERSION     DAOOLJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 51791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51791
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_458_length_51791_cov_14.407975"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLW16_03645"
     CDS             <1..320
                     /locus_tag="PLW16_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB70034.1"
                     /translation="RALHEASMPDIVILPMAELKTNYYFRFSAVDRPGVLSKISGILG
                     QKDISIAAVIQKGRKRDGAVPVVMTTHKAREADVQAALQVIDRLDVVMGKTQLCRIED
                     ESL"
     gene            354..1583
                     /locus_tag="PLW16_03650"
     CDS             354..1583
                     /locus_tag="PLW16_03650"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPB70035.1"
                     /translation="MKYAVLLGDGMADEPLDELNGRTPLEAAHTPYMDRIAAQGTLGL
                     LDTIPDGFTPGSDVANLSVLGYDPRECYTGRGAIEAANMGVSLGPSDVAFRCNLVTLS
                     DDASPVMEDFTAGHISTKEAGWIIRDLGEALSDGQGDFFAGVSYRHLYVRRNGNEAMR
                     TTPPHDIVGQPIDRYVPAGKGAGAILEIADRARGILRDHPVNRERRRQSRRPANAIWP
                     WGQGRAPKIEKMTAKYRISGGMISAVDLLNGMGIYAGLEVFRVPKATGYIDTDYEAKA
                     RTALEILDGLDFVFLHVEAPDEMGHEGNLTGKIRAIEDFDARIVGPVMNGLERGGPFR
                     LLVLSDHPTPIRLRTHAADPTPFAVLSSLDEENQRRGVAYGETAARANGLMVSPGYIL
                     MDAFIRDWRRFIDRASN"
     gene            1591..1968
                     /locus_tag="PLW16_03655"
     CDS             1591..1968
                     /locus_tag="PLW16_03655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB70036.1"
                     /translation="MIYADTDAMGIVYHANYIKWFEIGRTELMRACGIAHADIAPLGF
                     TLPVTRVYCHYLQPSRLDQVLAVLTTIDYVKRASVKFTYQIWDEALTERRVEGYTVHA
                     CTDPDGRVVRLPAPLVERLHAVR"
     gene            2005..2688
                     /locus_tag="PLW16_03660"
     CDS             2005..2688
                     /locus_tag="PLW16_03660"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB70037.1"
                     /translation="MATKINKKELKQPDILQSHFAQVLPMLQRQRTRIVQAGVVLGVI
                     VLLGCGWVLYEWSNSRAAAALYLKAEDGTMRASISDPSNREKALATYENIIEQYPRSS
                     SAMLARYRLAGYAHDQGQFDRAIEYYNAFIKQAPADSPLVALAGNGLGSAYEAKGNLA
                     QARQSFERALSSKAGDGFSGMTHQNLARVFEALNNSGQALTHYRKALEQVDDPALARL
                     LKRKIALLN"
     gene            2698..4500
                     /gene="iorA"
                     /locus_tag="PLW16_03665"
     CDS             2698..4500
                     /gene="iorA"
                     /locus_tag="PLW16_03665"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPB70038.1"
                     /translation="MKNRKLMSGNEAIARGAYECGVRFASGYPGTPSTEIMESFARYP
                     GVYAEWCPNEKVGLECAIGAALAGTKAMAVMKHVGVNVAADPLFTVSYTGTNGALVII
                     TADDPSLHSSQNEQDNRNYAKFAKIPMLEPADSAEAYAFIKIAFALSAQFDTPVFLRT
                     TTRVSHSKSIVEIADPLPVEDTTQIRFNAPKYVMVPAMARLRRIEVEKRMQALRAFAE
                     TFPENRAEINRTDVGVITAGIPYHYAKDVFPDYSYLKLGMVHPIPEKAIRDFADRIDR
                     LFVIEELDPFLEEQIRAMGIPVTGKDAFPYTNEFDPGVVERAMRTDPVEDPAYVPPVV
                     PPRPPNLCPGCPHRGLFYVLGKLKVFAAGDIGCYTLSFMKPLEGLHACICMGASIGMA
                     HGMSKALGEKGKGKVVGVIGDSTFIHSGITPLLNAAYNRSDCVFIICDNRTTAMTGMQ
                     EHPATGFTLQGQSAKALDFERLAEALGIDQVRVIDPYDIRQTRAVIREELARSGPSLI
                     VSRRACALFRRAAGPPQKPLRVDPGKCIGCRTCLGLGCPPISWRKFSDQAPDQIVKNT
                     KKQDGNAVIDPQLCNGCTVCQQLCKAGAIVAGEE"
     gene            4505..5110
                     /locus_tag="PLW16_03670"
     CDS             4505..5110
                     /locus_tag="PLW16_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPB70039.1"
                     /translation="MSKPKDRATGEKSVRSILMAGVGGQGILRASDILCEVMIRTGLD
                     VKKSEVHGMAQRGGCVTSHVRYGTCVHSPIAKKGDVGILIAFEKMEALRYLDYLRAEG
                     IAIVNDTEIYPPAVNLGEARYPDDILTLLPLPPASVKMVRAQEMGVRAGNPRTENIVL
                     LGVLSRYLTLEPSVWDEVLKVSFPAKLVDANLTAFHLGRVA"
     gene            5204..6160
                     /locus_tag="PLW16_03675"
     CDS             5204..6160
                     /locus_tag="PLW16_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPB70040.1"
                     /translation="MSEDYYKILGLEKAAAAEEIKKAYRKLALKYHPDRNPNNKTAED
                     KFKQVSEAYAVLSDPEKRQQYDQFGSAEQFRQRYSQEDIFRNFDLNDILRGFGFSFGG
                     AGGGGRVYTSGGRRRTYRQMDGDPFADLFGRGGAPYGGPTVPRAGQDLEYNLSITVEE
                     SVFGAEKKLALQKEDRSEEISFKIPPGIANGKKLRLAGKGAPSPSGGPPGDLYMNIQI
                     QPHPIFTREGNDLYIEKSVRFSQATLGTVLDVPTLDGGTKRIKIPAGTQHNTKIRMKG
                     YGAPGLKGAAKGDQYVKISVEVPKKLTDRQTKLIRQLDEEGL"
     gene            complement(6203..6991)
                     /locus_tag="PLW16_03680"
     CDS             complement(6203..6991)
                     /locus_tag="PLW16_03680"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPB70041.1"
                     /translation="MNGQTEREGRPSGRDAPGHRYEFRTYRNRIAPEGNLAAFSVCVR
                     ETDLFLRADRDLSHETLETVHACRAQIETYILHHPEFLHARTPLPEDPLAPDIVRRML
                     LAARRAEVGPMAAVAGAIAESVGRSLRTLPGISNVMVENGGDIYLDCKRDLRVEVHAG
                     QSSLSGKIILMIRSDRMPLGVCTSSGTVGHSFSYGRADAVCVLSVSAALADAAATRIG
                     NLVKSAKDIERGLKTAEEILPGIRGVVIICGSHMGLAGDVELAG"
     gene            complement(6988..10455)
                     /locus_tag="PLW16_03685"
     CDS             complement(6988..10455)
                     /locus_tag="PLW16_03685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB70042.1"
                     /translation="MSDTARFTHLHLHTQYSLLDGTIRLDDLFRKAHDFGMTAIAMTD
                     HGNIYGAVDFYQRAVKAGIKPILGCELYVAPQSRFTKGGQGIGESSNHLVVLVENAEG
                     YDNLKKLTTAAFLEGFYYRPRVDKELLARHHKGLIGMSACLHGEIAERILRGDEGGAA
                     KAAEEYRQIFGAGNFYLEIMQNGLPEQTQANEGLRALSRRLDLPLVATNDCHYLERKD
                     AEAHEVLLCIQTGKTLGDADRMRFATDAFYFRSPDEMTALFADCPEAIAHTAVIADRC
                     NYQFDFGKFYLPHFSLAADETLDERLEHSALEGLEALLPDIVPAEDAVLSERYYERLR
                     DELAMIRTMGFAGYFLIVADFVNYAKKNNIPVGPGRGSAAGSLVAYAIGITSIDPIRY
                     RLFFERFLNPDRISMPDIDIDFCQEGRDDIIRYVTEKYGADHVAQIITFGKMQAKAVV
                     RDVGRALSIPYGEVDRIAKLIPNILNIKLDEAIQMEPRLQEAQQKDERIRKLLELSLA
                     LEGLNRHSSTHAAGVVISDVPLVERVPLCRSPKDDVVTQFSMNDLQEVGLTKFDFLGL
                     KTLTVIKNTLRFIREGRGVELNMKDLPLNDEKTYRLLMRGETDGVFQLESSGMKDILV
                     NMKPDCIEDVIALIALYRPGPMGMVPEFISRKQGKTKIAYEVKELEDILRETYGVILY
                     QEQVMQIASVIGGYTMAQADTLRKVMSKKIVSAMEKEKPRFLSGAKQNEIPEQKAIRI
                     WEQMESFAAYGFNKSHSTAYAMISYQTAYLKAHYPAEYMAALLTSEKDNRDKIIKYIG
                     VCREMGIQVLPPDINASGRDFSVSGDHIRFGLAGVKHVGMGAIETIVQARDATGEFRS
                     LFDFCSRVDLRKVNRRVIESLIKCGAFDAMGCKRRPLLMSLDELTERIHVRQRDAESR
                     QASMFTDTDESGETSAKSLEEAIPDLPEWEHKERLAYEKDTLGFYITGHPLSKYSERL
                     AMLADTDSVSLSERRDKETVAMAGVVNALREVKTRRKDTMAYVTLEDLKGSFECIFFS
                     EIYRKGYDLLHGDDPLLVRGTIDVGEETVRVMATEIEHLEEATDRPFSSVHFHMTARS
                     VTVETLLSLRDLLIRDYPGKCDGFLHVHDNGTETVVSLGDETRVTASTGLRETADGLI
                     GLRGATIFQ"
     gene            complement(10516..11976)
                     /gene="guaB"
                     /locus_tag="PLW16_03690"
     CDS             complement(10516..11976)
                     /gene="guaB"
                     /locus_tag="PLW16_03690"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPB70043.1"
                     /translation="MLDDPIREALTFDDLLMVPAESAILPGNVETATCLTPRIALNIP
                     IISAAMDTVTEATTAICMAQEGGLGIIHRNMSIERQAIEVDKVKKSESGMIVDPITIE
                     PEQRVHHALELMNKYRISGVPVVKDGRLVGILTNRDLRFETDLDQPVSDVMTKENLVT
                     VTSSISLDESKKLLHKHRIEKLLVVDDQYRLKGLITIKDIEKIKKYPQACKDSLGRLR
                     VGAAVGILDREPRVDALLAAGADVIVIDTSHGHSHGVIEAVRATKSNFPGCELIAGNV
                     ATAEGAESLIQAGVDAVKVGVGPGSICTTRVIAGVGVPQMTAIRDAYRAAAKHGIPVI
                     ADGGIKYSGDVVKALAAGAHVVMIGSLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KMGSRDRYYQDDMEGDLKMVPEGIEGRVPFRGSLSASIQQLIGGIKAGMGYVGCQTLP
                     DLHKKARFVKITSAGLRESHVHDVIITKEAPNYWLD"
     gene            complement(11980..12474)
                     /locus_tag="PLW16_03695"
     CDS             complement(11980..12474)
                     /locus_tag="PLW16_03695"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HPB70044.1"
                     /translation="MAQTDTTTPRTDIRIDKDGVWFYRGQEMFRREIVNLFYEHLKQD
                     ERGEYIIDMGEDSCYVEVEDAPFVVRAVLTGPAERETSPACLLLSLSDNTLEPLDPET
                     IRIGDDNVMYCRVKGGTFKTRFSRAAYYQLAERIVYDAASERYELHVDQKRYTLQTPR
                     EKEN"
     gene            complement(12490..12936)
                     /gene="dtd"
                     /locus_tag="PLW16_03700"
     CDS             complement(12490..12936)
                     /gene="dtd"
                     /locus_tag="PLW16_03700"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPB70045.1"
                     /translation="MRAVVQRIERARVGVDGGTVSEIGKGVLVFLGIEKGDAEKDAEY
                     LFQKIVHLRLFDDAEGKMNLSLEDIKGEMMVISQFTLLADCRKGRRPSFIAAEDPAPA
                     RLLYEYFIRIGRDRLGLIGSGQFQAMMKIDSVNDGPVTLILDSRRV"
     gene            complement(13042..14148)
                     /locus_tag="PLW16_03705"
     CDS             complement(13042..14148)
                     /locus_tag="PLW16_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70046.1"
                     /translation="MSSVNSVLADMISSVDLLKITGISRATMNNYIKLGLLPKPVVGK
                     PRDPEMRARQVGYFPSDVLSTLEKVHILKKDGKTIPEISTALGVGIFASKSSASSAAR
                     RTSPPREEQTSIHALQTELFPEIAETSETFEADGLQSAISPGDDALTPPSPQDWRSFC
                     ILSARIQDYPQLRAELPPEEFFEFLRRFRTDVDAAAQRVRTTHSTYSQDGLLCFFIQG
                     DDDHYLLDAIHFSLELRRIAQGLNAAWKRRKKWGNHLFVNVGVVDGSQWVTRFAAGSN
                     RDVFLSGAEVEQARCLCDFARFGSIWTTKTLLGRLSPSDRQGLRYGIRTQGVSGEFWT
                     EQVYARIMDLFSPDDPHVMKYLDIISIPITEIIG"
     gene            14277..15728
                     /locus_tag="PLW16_03710"
     CDS             14277..15728
                     /locus_tag="PLW16_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70047.1"
                     /translation="MDELFIFMYYIIIDHFITHPVFVCLGHSCMVRSAMQRPKHILFV
                     TPWIYDFAAFDFWMKPLGLLSLAALFRDNGYSVNYLDCLMDHDLADQAHRKTRRTEHG
                     TGHFDKEAIVKPAPLAFFQRRYYRYGIPMGLFRRKLLSIPPPDMVLVTSMMTYWYPGA
                     FEAIHLLRASLPGVPILLGGNYATLCPDHARQSGADRIFPGPGEWHFSEIIKTFFNDR
                     PRILPDFDDLDTYPRPAFDLLGRLDQVPLLTSRGCPFRCSYCASNQLYPKFMRRHPLR
                     VAEEIFDWHDRFGVRHFSIYDDAFLVDPPSLALPLLDTIVQRKANLSFHCPNGLHLRQ
                     ITPVLSRMLYRAGFRTLRFGFETFDPDRQATTGGKVNNDHLIAAVRYLCEAGYEASDI
                     GVYLLCGLPGQTAEEIDGSIRFVQSLGAKPYLAEYSPIPGTALWPEALQSSPYDLLNE
                     PLCHNNTLLPCAGDTLPEPAYRELKLKTRMSPR"
     gene            15805..16029
                     /locus_tag="PLW16_03715"
     CDS             15805..16029
                     /locus_tag="PLW16_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70048.1"
                     /translation="MSAFSIGRCIRQFALFLISSLFFLFGIYVMIRTYHLENPLEFVM
                     AFFSSSLIILISLVGMISPSVQVYLAWRKR"
     gene            16034..17275
                     /locus_tag="PLW16_03720"
     CDS             16034..17275
                     /locus_tag="PLW16_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPB70049.1"
                     /translation="MNLQTFKRLTDRIEGYEPEMVQMQIDLCSYPALSPDNGGDGEQE
                     KALYLQGSMRRLGLTDIETLPAPDSRVSSGLRPNILVRLPGHQPDKTVWILTHMDVVP
                     PGEPSHWASDPYRAEVRNGRIYGRGTEDNQQDLVASIFALRAFYELGVTPEYNLGLAL
                     VADEETSSRFGVRHLLDQVKPVFRKNDLIVVPDFGDAEGTAIEIAEKSLYWLRFRTTG
                     RQCHGSRPDLGRNAFAAAAHLVVRLRELYDLFPLRNPLYEPQESTFEPTRRDANVPNV
                     NTIPGDDVFYLDARVLPEYPLTRVLEAIRALADDIEQIHQVGIVIETVQSLPAPPPTP
                     ADAPVVAALREAVHRVYGLHAKPVGIGAGTVAALLRQEGYPVAVWSRQNETAHQINEN
                     CLISNMIGNAKVFAHLGLNPV"
     gene            17297..18277
                     /locus_tag="PLW16_03725"
     CDS             17297..18277
                     /locus_tag="PLW16_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPB70050.1"
                     /translation="MQSLTEKLTFDDNPSLKVLLGERDVHVRQVERLTPVKVSLRGNQ
                     LSIEGGAPDVELVRSLLLQMYALARKGFPIYPQDILHAHRILSENRQTDLEKIFLDTV
                     FISSKKRAITPKSIAQKRYIDAIRDCDMVFGIGPAGTGKTYLAMAMAVAALQDKKVHR
                     IVLTRPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALFDMMDFDRASALIHKGTIEVAP
                     LAFMRGRTLNDAFVILDEAQNTTSEQMKMFLTRLGLGSKAVITGDITQIDLPQEIKSG
                     LVEAETLLRSVQGIRVVLFSELDVVRHPLVQEVIRAYQRLNGTGPRFNGP"
     gene            18334..18786
                     /gene="ybeY"
                     /locus_tag="PLW16_03730"
     CDS             18334..18786
                     /gene="ybeY"
                     /locus_tag="PLW16_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPB70051.1"
                     /translation="MPVLIESRQTVRRINRVRIRRILTEALVLLGCSDHEVSVLLVDD
                     IGIQTINRDYLGRDRPTNVMSFAIREGEYGALNPHLLGDIVVSVEKAWSDARQEKIKF
                     TDELDFLLIHGLLHLLGFDHEDVPATKARDMKAMERRIFRDLKGYEIQ"
     gene            complement(18799..20625)
                     /locus_tag="PLW16_03735"
     CDS             complement(18799..20625)
                     /locus_tag="PLW16_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPB70052.1"
                     /translation="MMTFVGRSVVSCLSLFIAMQLTLVTPSVADEGNTLAQAVRQTET
                     RGEAAILSENVLPSSDAKAIGNPSGAARLKSGAPASEGNDDKNDPQKIEDEERDIMEE
                     ALALLEESNAHWQKGDLENAQNLLDEAYALIIETDGDTDISRQKDDLRLLIAQRILAV
                     YSSKQNVTAGKRSEIPMRINEDVEKEIRLFQSVERDFFISSYQRSFKYRPMIVRELKK
                     AGIPEELSWLPLVESGFKASALSRARALGLWQFIPSTGYKYELNRDEWIDERMDIEKS
                     TRAAISYLKDLHGMFGDWLTVLAAYNCGEGRVLRVISRQHVNYFDRFWDLYHQLPYET
                     ARYVPRFLATLAIIKDPQKYGMDLGSPSPNEDLSTYEIVKTTRSMRLSDIAARLEKPE
                     ELLVCLNAELRHRATPSREYTLRVPTEMAEKFAKIEAEIPAWEEPKPTVVAKKTVTIR
                     HRVKRGETLTSLARKYRTSASAIRSANRLSSNKRLRAGRIVRIPIPSYRYEKIQASSA
                     DTGKTLTHRIRRGETLSSVARRYGVSVSDLKRTNRLRSNTIRTGQILRIAKRAAKTYV
                     VKKGDTLTKIALKNGTTLKALKEMNGLGEGGHIYPGQSIVLE"
     gene            complement(20729..21818)
                     /gene="prfB"
                     /locus_tag="PLW16_03740"
     CDS             complement(join(20729..21751,21753..21818))
                     /gene="prfB"
                     /locus_tag="PLW16_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPB70053.1"
                     /translation="MSETAFKLEGLRDKITRIGECLDVPTLNLRRRELELASQRADFW
                     NDPEKAREILKEKTTLDETVERWDRQQRALDELADLYSLAETEQDAAVLSEIDTELER
                     LDKDVRQDELKRMLGHEEDTLNAIVSIHAGAGGTEAQDWAEMLLRMYLRWAEKRKFTP
                     TVIDSLPGDEAGIKSVTFILEGEYAYGYAKAENGIHRLVRISPFDAGARRHTSFASIF
                     VYPEIDDQIVIDIDEKDLRIDTYRSTGAGGQHVNKTDSAVRITHLPTGIVVQCQNERS
                     QHKNRSMAMKYLKSRLYELKIKERNEKQDAVNKNKKDIAWGSQIRSYVLHPYRMVKDH
                     RTNLEVGNVNRVLDGDIDDFIEAYLLGG"
     gene            complement(21799..23361)
                     /gene="lnt"
                     /locus_tag="PLW16_03745"
     CDS             complement(21799..23361)
                     /gene="lnt"
                     /locus_tag="PLW16_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPB70054.1"
                     /translation="MKADNTVSGTPSAFNPGPLFGAVLTGVLLFLSFPKFGCGVIAWV
                     ALVPLIISLRGKGTREAMILGFTAGLVFHVGILYWITYVVVKYGSLSPPLGVAAMLLL
                     CAYLSLYTAFFTAGCVFFRHCAPLIAVPLLWTGLEFIRGWIFTGFPWEALAYSQYRCL
                     PLIQIVDVTGPAGLTFLIVLVNMLLFQVLTTKLASMRFASIVAIAVVFSLLAGYGLWR
                     LGVVRNGIAKSASIPVSLIQGNIDQNLKWNPRYQRNTVDAYLSQSARHLPAAGGVIVW
                     PETAVPFYFQDDGPLQRRILAFARDHRVWMILGSPTYADRALGTNFANSAFLVDPAGR
                     IAGRYDKVHLVPYGEYVPLRRYFPFISKLAVGVGDFRPGRGYVPLPMNGHRLGILICY
                     EGIFPAAGTDYKRRGAEMLVNITNDAWFGPTSAPYQHLSMTVFRAVETRMYLCRAANT
                     GISAFISPTGKITKKTGIFQRSGLSGEVRLFQKKTIYSTYGDFFAYSSIIVLLGMTIV
                     AWMRRKHERDRI"
     gene            complement(23348..24130)
                     /locus_tag="PLW16_03750"
     CDS             complement(23348..24130)
                     /locus_tag="PLW16_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB70055.1"
                     /translation="MPAKDHGRLEKELQSIIASGEEEGLLDPQSGEMIQSILDFRETL
                     VREIMIPRTEIVAFSTTASIGEILALLQKHGHTRMPVYKGSIDNIVGIINVKDLHKFW
                     SRNVTEADVLSILRKPYYIPETKNTHQLLHELKQNKYHLAIVIDEYGGTSGLLTLEDL
                     IEEIVGEIHDEHDQNGDELIELADGSVLANARLEIEVLEAHYGLAFDDGEYETLGGLI
                     LHVLKRIPIPGEIVRIGPLEMKIEAADERSIKKVRIRKSESR"
     gene            complement(24195..25067)
                     /locus_tag="PLW16_03755"
     CDS             complement(24195..25067)
                     /locus_tag="PLW16_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70056.1"
                     /translation="MKYEGLIIRPPSEAYSLLLQVTTGCSHNKCTFCPTYRDKKFRIK
                     SREEILEDLEEASHFRRVKRVFLCDGDALIIPQPRLLDILALIRRHLPGIERVSTYAN
                     AKSILRKTAKDLLALKEAGLKMVYLGVETGSAALLEKIHKGVGVEQMVDAGRRIKDSG
                     ILLSVTVLLGIGGRAHSIEHATETAKILTRIDPDFTGALTVMVVPGTPLYEDMQAGRF
                     QLPDSFGFLTELGTMIARSQFTDCFFTSNHASNYLPIRARLPQEKEETVRMIQEVVQR
                     GDRGLLRPESLRAL"
     gene            complement(25172..25248)
                     /locus_tag="PLW16_03760"
     tRNA            complement(25172..25248)
                     /locus_tag="PLW16_03760"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25212..25214),aa:Asp,seq:gtc)
     gene            complement(25334..25409)
                     /locus_tag="PLW16_03765"
     tRNA            complement(25334..25409)
                     /locus_tag="PLW16_03765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25374..25376),aa:Val,seq:tac)
     gene            complement(25485..27923)
                     /gene="lon"
                     /locus_tag="PLW16_03770"
     CDS             complement(25485..27923)
                     /gene="lon"
                     /locus_tag="PLW16_03770"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB70057.1"
                     /translation="MKHREDSSLLKVPLLPLRDVVVFPHMIVPLFVGREKSIAALESA
                     MKFEKGIFMVAQKNAQKDEPTENDIYRVGTLGIIIQLLRLPDGTVKVLVEGKQRAGVR
                     EYLPSDDFFFVAVEEIPDVDDPNSVRAEALIRSIREAFETYAKLSKKIHMEIIGTIAS
                     IDEPSKLADIVVSHLNVKLEDKQKILEIFNVTDRLETIYEMMLAEIEILEVEEKIKRR
                     VKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFKNEIAELEKKLKQKKLSEEAHKKVKSE
                     LKKLQMMAPMSAEATVVRNYIDWLLDLPWGEKTENHYTLKESETILEEDHYGLKKVKE
                     RILEYLAVQTLVQKNKGSILCLVGPPGVGKTSIAKSVARATNRKFVRFSLGGVRDEAE
                     IRGHRRTYIGALPGKIIQLMKKAGSNNPVLCLDEVDKLSSDFRGDPSSALLEVLDPEQ
                     NNAFNDNYLEVDYDLSDVMFITTANVLQSIPAPLQDRMEVIRLAGYTEMEKLRIAKKF
                     LIAKEREANGLRENQISFSDGALLTIIRKYTREAGVRNLEREIASICRKVAREIVTDV
                     SVKKISITSKSLDKYLGIPQYRHGETEGQDRIGTTVGLAWTEVGGELLIIEATTLRGS
                     GKITMTGKLGDVMQESVQAALTYVRSRSDQFYLDENFYKETDVHVHVPEGAIPKDGPS
                     AGVAMVTSIASAFTKQKVRGDLAMTGEITLRGRVLPIGGLKEKLLAAHRGNIKTVLIP
                     KDNEKDLADVPPNILKALNVVLVEHIDDVLASALVPKVEPTGDVSTEVRIVPGVRDER
                     PSAVLAPVSQKAAS"
     gene            complement(27972..29234)
                     /gene="clpX"
                     /locus_tag="PLW16_03775"
     CDS             complement(27972..29234)
                     /gene="clpX"
                     /locus_tag="PLW16_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPB70058.1"
                     /translation="MTKKGRTQQEGQGMLYCSFCGKNQNEVKKLVAGPSAYICDECIE
                     LCKCIVDEDASPSVVDQAGQRIPEPADIKRFLDQYVIGQDRTKKVLSVAVYNHYRRLF
                     TDVDMEGVEIEKSNVLLIGPTGSGKTLLAKTLARMLNVPFTIADATTLTEAGYVGEDV
                     ENIILSLLQNSDYDVARASKGIVYIDEIDKIAKKTGNPSITRDVSGEGVQQALLKIIE
                     GTMANIPPKGGRKHPQQEFIQVDTSNILFICGGAFNSLENIIAKRTGVNTLGFGADIR
                     SKKSQSIGELLTVVQPEDLLKYGLIPEFIGRLPVVATLNELMEEDLVRILVEPKDAII
                     RQYQKMFEMEKVKLKFTDGALRAIANLSAGRKSGARGLRAVIENTMLDIMYDIPSRLD
                     VRECVINEDVVLSGAQPILLFENKSESA"
     gene            complement(29262..29846)
                     /gene="clpP"
                     /locus_tag="PLW16_03780"
     CDS             complement(29262..29846)
                     /gene="clpP"
                     /locus_tag="PLW16_03780"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPB70059.1"
                     /translation="MPLIPMVIEQDGRGERAFDIYSRLLKDRIIFLGTAIDDDVANLI
                     IAQLLYLESEDPDKEIYFYLNSPGGHVSSGLAIYDTMQYIKPSVCTVCMGQAASMAAL
                     LLAAGQKGKRYALPHTRILIHQPLGGFQGQATDIDIQAREILRLKEELNIILSSLTGQ
                     ELSKIMTDTERDYFMTPEQAKQYGIIDEIIAKRA"
     gene            complement(29847..31175)
                     /gene="tig"
                     /locus_tag="PLW16_03785"
     CDS             complement(29847..31175)
                     /gene="tig"
                     /locus_tag="PLW16_03785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPB70060.1"
                     /translation="MEEQILDVKVEEITPVKKKFSFVIPWENVKKELDQVYRDVGKKA
                     KIKGFRPGKVPLPVLKTHYKEYAEGETVQNLVNRHYREAIERERLTPVSQPEVDPKSI
                     EPEQALLFTATVEVEPVVEPKNYAGLELEKIQTDVTDADLEARLLQIREMYGTLEEIS
                     DDRELQEKHFATIDFSGTIDGQALDELKSENYFLEVGSHTFIPGFEEQLVGMKKGETR
                     DIQVKFPDDYGQAQLAGKDVRFSVSLKGIREKKLPPLDEEFVKNFEQYDSLEALMADV
                     RSSLEGDQKGRDEAAFQEVMVDALLKENPFEVPMSLVERQTFYLMTDMQRRMTWAGMD
                     KQQAAELSFGMHEKLKEQAGKIVKTMFLMRAIADREGLTVTEEDLDAQLHEMAAKLGK
                     DIPDLRSKLAEEGSLEQVRMDLLQRKTFRFVEEKATVTIRNAAASEGEDR"
     gene            complement(31229..31314)
                     /locus_tag="PLW16_03790"
     tRNA            complement(31229..31314)
                     /locus_tag="PLW16_03790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31278..31280),aa:Leu,seq:tag)
     gene            complement(31421..31497)
                     /locus_tag="PLW16_03795"
     tRNA            complement(31421..31497)
                     /locus_tag="PLW16_03795"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31461..31463),aa:Pro,seq:ggg)
     gene            31651..32259
                     /locus_tag="PLW16_03800"
     CDS             31651..32259
                     /locus_tag="PLW16_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPB70061.1"
                     /translation="MNLKRNDTPWRWIGAAMLAACLSAGSPAVADIYKYVDPDGMIHL
                     TNVPTDDRVRYELLIREKRVLFRPGTDIKKYDHIIDRASEKYRVDPALIKAVIKAESN
                     FNHRAVSPVGARGLMQLMPKTASSLNVADSFHPENNIEGGVRYLRYLLNLFNGNLPLA
                     VAAYNAGEGAVSRYQNTIPPYPETQTYVRRVLDFYRQYRQIN"
     gene            complement(32282..32857)
                     /locus_tag="PLW16_03805"
     CDS             complement(32282..32857)
                     /locus_tag="PLW16_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB70062.1"
                     /translation="MLTGRQQTGGITLLVVCLLSILGAVALTSGEKKTADPSPETRSS
                     SLEIRLAGDARRHGVYFVPAGSTAFDVLKRSGEWDNDPHRISLPAGLLRPLNRGDLVQ
                     VVTAPDGKRWIRLSQMPAAERMTLGIPLDVNTASVEELTLVPGIGPKTAFLIVRERDR
                     RKVFHALEELAAVPGIKGKRLEGLRPYLTAD"
     gene            complement(32866..34089)
                     /locus_tag="PLW16_03810"
     CDS             complement(32866..34089)
                     /locus_tag="PLW16_03810"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPB70063.1"
                     /translation="MSLIVQKYGGTSVADIEKIRNVARRAVRVREMGNQVVVVLSAMA
                     GETDRLIRLAQSACPNPPSREYDALIATGEQVSVTLLSMILNEMNHPAKSFLGFQVRI
                     RTDSAHAKARILDVETAGIAQALRDGIICVVAGFQGVDERGDITTLGRGGSDTSAVAL
                     AAALKADVCDIYTDVDGVYTTDPNLCSKARRIDRISYDEMLEMARTGAKVLQPRSVEL
                     AKKHGVPICVKCSFSDEGGTLVTKEDKDMEKEVVSGVTYDRDQAKLTVVHVPDRPGIA
                     GKIFTPLSAQNIVVDMIIQNASLEGFTDLTFTVSKKDSREAYRLLQDVCREIGAANVD
                     LDDNVAKVSIIGVGMVSHSGVASRMFTALAQEGINILMISTSEIKISCVVQAKYTELA
                     VMVLHDTFDLEQKRN"
     gene            complement(34094..34573)
                     /gene="tsaE"
                     /locus_tag="PLW16_03815"
     CDS             complement(34094..34573)
                     /gene="tsaE"
                     /locus_tag="PLW16_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB70064.1"
                     /translation="MNERLDVVCPSPEETRRLGEAIGRTLTAGSIVALSGDLGAGKTT
                     LAQGIARGLDIPETFAITSPTFTLVNEYPGRLTLYHVDLYRLGGAADLETLGYEEFFF
                     ADGVTVVEWAEKISDILDEGCIFIELRYIDAGRRHIGLRAAAAIVRRVDDIWKGGTK"
     gene            complement(34566..34991)
                     /gene="acpS"
                     /locus_tag="PLW16_03820"
     CDS             complement(34566..34991)
                     /gene="acpS"
                     /locus_tag="PLW16_03820"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPB70065.1"
                     /translation="MIAGIGIDLVEIARIERIATRWGDAFHRRVFTLREIDACSERAR
                     PAIHFAARFAVKEALLKSLGLGLGMGVRLHEIETVNERDGRPTLLLHANALWILERRG
                     IGRTHLTITHTKDHAMAVVVLEADGRRPRGPELDADPYE"
     gene            complement(34988..36103)
                     /gene="folP"
                     /locus_tag="PLW16_03825"
     CDS             complement(34988..36103)
                     /gene="folP"
                     /locus_tag="PLW16_03825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB70066.1"
                     /translation="MLPKMRSLLIALDDVECRVANILKQEMLASGGDAAVHRESVACT
                     NPSTDVLIMGTPKQIRRLAAKLAVQPFGLSGLSRSLIEILENSECTDYLLRTGHREMT
                     VGKTTAVMGIINVTPDSFSDGGQFPTVDAAVQYGYRMAEEGADFIDVGGESSRPGADP
                     VPADVELARILPVIEALHACVEVPVSVDTTKAEIARHALAAGAEIVNDISAMTYDPAM
                     PAVVAESGAAVVLMHMRGLPKTMQAALSEYRSLPGDIVLYLKERLEAAQTSGIGFDRI
                     IVDPGFGFGKSLGDNLALLNGLAELRMLGRPILAGVSRKSFVRQITGETLSASSEGTA
                     AAIAAAAMNGAAIVRVHNVPAARKVVSMIDALKEART"
     gene            complement(36227..38056)
                     /gene="ftsH"
                     /locus_tag="PLW16_03830"
     CDS             complement(36227..38056)
                     /gene="ftsH"
                     /locus_tag="PLW16_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB70067.1"
                     /translation="MNPLQKNIALWLVISLVFVLLFHLFNEPKATQGNIVYSDFLTHV
                     ERGQIGEVQVQGENITGKLTTGKTFKTYAPKDDDLIPLLRSKGVRIAAKPVEDSPWYM
                     TILISWFPMLLLIGVWIFFMRQMQSGGGKAMAFGKSRAKLMTDKSKKVTFADVAGIDE
                     AKAELQEVIDFLKDPKKFTRLGGRIPKGLLLVGQPGTGKTLLARAIAGEADVPFLSIS
                     GSDFVEMFVGVGASRVRDLFTQGKKQAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFESNEGVILVSATNRPDVLDPALLRPGRFDRQVMVPLPDVRGREKILQV
                     HAKKTILDEDVNFAVIARGTPGFSGADIENLVNESVLYAARMGKERVGMRDFEFAKDK
                     VLMGAERKSMVISDSEKRNTAYHEAGHTLVAKLLPGTDPIHKVTIIPRGRALGLTQQL
                     PIDEKHTYPRKYLLNNIAILLGGRAAEALVLNDFTTGAGNDIERATHLARKMVCEWGM
                     SEVMGPLSYGKKEEQIFLGREFATHKDYSEQTAEKIDQEITRIVSDAYEKSKTLLMDH
                     MDILNRMASALLEKEVLNAEEIDALVFPPDADDTAGGAAAIPA"
     gene            complement(38189..39619)
                     /gene="tilS"
                     /locus_tag="PLW16_03835"
     CDS             complement(38189..39619)
                     /gene="tilS"
                     /locus_tag="PLW16_03835"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPB70068.1"
                     /translation="MFVRALEKTVRRRGLLERGDCVLVAVSGGPDSVALLRGLMDLRS
                     RYDLSLTVAHFNHGLRGRASDAEERFVSELAVKWGVELRTGRMTTASLRPRGRSLEDW
                     CREHRYAFLDATAREIQAGKIALGHHRRDQAETVLMNLLRGSGIEGLKGILPIRDGRY
                     IRPLLDMDPADIRTFLKCRSLSFRNDASNRDVHHLRNRIRHDLLPQLEAVYNPAIVRG
                     LTRMAEIAGLENGFMESVLPEAYARAGGREGQGRIRFDIPAFLTLHEAIQNRLILHVV
                     RRLVGAPSGITHAHIQLVRNLLGRYGYAEVHLAGGITVTRSYDQLSFQKTDDVSLPAS
                     KSQHRPSRLRGKSTDAEAGFQFDVPVPGVLTLAPGCRLRFEVAGVTRPVPTGSQIACL
                     DYERIRFPLVCRGVRTGDRIQPLGMAGTKKVRRLLLDAKVPLEERGSVPLLVDDDGVL
                     WVAGVCLSERARVSDRTRTVLKAEII"
     gene            complement(39676..40329)
                     /locus_tag="PLW16_03840"
     CDS             complement(39676..40329)
                     /locus_tag="PLW16_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPB70069.1"
                     /translation="MNAKKHAVSLMIFDLDGTLIRSGDDIAASVNYTLQALSQPTIER
                     EKILRFIGDGVRVLIERSLGHAAPELVAKALDLFSAHYAGHMLDTTDLYPDVHPVLTY
                     FARQNKVIVTNKPIAAARTIVDRLGIRGYFTGIIGADTTHRKPDTGVLAPLYEIVAPD
                     PKRTLVVGDGVNDILLAKNAGFISCALLNGLSDRDDLLALEPDHACERLSELVDLFA"
     gene            40685..41581
                     /locus_tag="PLW16_03845"
     CDS             40685..41581
                     /locus_tag="PLW16_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPB70070.1"
                     /translation="MEKDTFAIIGLGKVGTAVGLLLQASGYPIVAVSDVDAAALKRAS
                     RFPGAHAVEACSEAAALAHCILITTRDDRIGPVCEEICRNGGIRPGDICCHMSGAGGL
                     DLLAPARRFGAQVASIHPIQSFADVDGAVANLPGSTFGVTADPSILDWACQLVRDLGG
                     VPLVVAESEKTLYHAAACVASNYLTTLLSMVEDLYRSLGVDAQSARQAFWPLVLGTLR
                     NIEVRGPVEALTGPIARGDVGTLRRHLAALRTGKPEFLRMYCLMGLMTVELSQRQNRL
                     SPEILIEMKDLLKGGCEDEHSR"
     gene            41565..42380
                     /gene="panB"
                     /locus_tag="PLW16_03850"
     CDS             41565..42380
                     /gene="panB"
                     /locus_tag="PLW16_03850"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPB70071.1"
                     /translation="MSTHGKLNKVTSATVRDMKRRGEKISMLTAYDYPTALCIDEAGI
                     DMILVGDSLGMVVLGYDSTLPVTMEVMLHHTRAVSSAVQRAMVIADLPFMSYQCSPDD
                     AVRNAGRMLQESGAQGVKLEGGRDFAEATRRMVAAGIPVMAHLGLTPQSVHQLGGYRV
                     QGKDDVGARRILEDARILEEAGAFSLVLECVPASLAADITAALAIPTIGIGAGPRCDG
                     QVLVLHDVMGMFERFTPKFVKKYAQVNALMKEAAQAYITDVREGGFPGPEHSF"
     gene            complement(42395..43465)
                     /locus_tag="PLW16_03855"
     CDS             complement(42395..43465)
                     /locus_tag="PLW16_03855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB70072.1"
                     /translation="MRQKPLWGVVAVLLGLTLLLSACGSKEEKAAPLSGGAGVQAPPS
                     SAPQSAEIPTPRLGPAGAVVEVDGSILTEKQLDRELKEKISGMKGKIPAAKIEEARKG
                     LRQRVIEDFVVRTLLSNEVRRRNIQIADREVNEAMEQLQASFPPGTTLESQLKKNKIT
                     KVKLREEIDFGLKINKLVLSQPKAKEKATEKEIQGFYRKNSDKFRSPESVRVRHILIA
                     KAAGEDAAAAQTKKNKAEDLQKQLAAGGNFAELATTNSDCPSKESGGDLGTFTRGQMV
                     KPFEDAAFSQKINAIGPVVETEYGYHVIQVLEKHPPKTMPLDAQAKEMISSYLQRQKQ
                     QAAFNHLVRELREKANVVLYQP"
     gene            complement(43643..44935)
                     /locus_tag="PLW16_03860"
     CDS             complement(43643..44935)
                     /locus_tag="PLW16_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB70073.1"
                     /translation="MTVVIPALAEKGHLFHTLAALAANPADQLAETLVIVVVNNGPRE
                     TVDREMFTDNQETIAILNGLIAGRTRVSEYTDGGLRGVIDFLRGSGLRIGLIDASSTG
                     CEIPSSLAGVGTARKIGLDLALTLRPSPRERPVLVCLDADTIVAPDYLPAIRRHFHDP
                     EHGAAVIAFNHPLPDDTDRRAAIVCYELFLRYYVLGLFWAASPYAFHSIGSTMVFTPD
                     RYVAVRGMSRRTAGEDFYFLNKLRKAGPISSLTDTCVRPSDRISSRVPFGTGKRMIRH
                     LAGERDEYLFYHPEIFAILRKWLALMDEGWQLQAKEILEAASSLHPGLRGFLERQNFA
                     EVWPRIRRQVREYAAAQRAFHTWFDGFRTLRLVHELAASAYPPQPMAAALSWLMNVWG
                     QNGPGPGEAGFSGNLQAQEACLMTLRSVEAPNVLRGAF"
     gene            complement(45007..45945)
                     /gene="miaA"
                     /locus_tag="PLW16_03865"
     CDS             complement(45007..45945)
                     /gene="miaA"
                     /locus_tag="PLW16_03865"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPB70074.1"
                     /translation="MTVNLVAILGPTACGKTALGVRLAREIGGEILSADSRQVYRGLD
                     LGTGKDLDEYRSEAGVVPCHLIDIVAPDDEFHVFAYQRLFFQRFDEIRNRGAFPILVG
                     GSGLYLEAVLMNYPFEEVPPNEALRAVLSGLDNGQLSERLRRSRPDLHNTTDLVDRDR
                     LIRAIEIAEHAPSGPLDSGYRPTDLNPLVIGIAMEVDALRERIARRLSQRLEAGLVAE
                     VRRLHDDGLSWESIDRFGLEYRYVARFLQGRINADRLYEELNTRIHQFAKRQRTWFRR
                     MERRGIRIHWIPGDDYPALRKLVLAHLEGMTVGNKG"
     gene            complement(46020..46220)
                     /locus_tag="PLW16_03870"
     CDS             complement(46020..46220)
                     /locus_tag="PLW16_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB70075.1"
                     /translation="MAEGKVKWFNEKKGYGFIESDEGGDVFVHYSAIQGSGFRTLYEG
                     QRIRFDIEEGKKGPAAANVQTV"
     gene            complement(46422..47042)
                     /locus_tag="PLW16_03875"
     CDS             complement(46422..47042)
                     /locus_tag="PLW16_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB70076.1"
                     /translation="MMEEAVSIDVGSIRIEGRMAHGRGRGGVVVSHPHPQMGGSMDNN
                     VVEAVCEGFRCLGFTTLRFNFRGTGRSEGRYDGGRGEQDDLRAAMDFLAGRTSAPLLL
                     CGYSFGAWVTASLYAREPLACRLLLVSPPIDFLETPLDPLAEGLIGVICGDHDTFCDV
                     QELTERLGRINLVPQVLTGTDHFYIGREEALVIAIKTILNSFLKNA"
     gene            complement(47039..48007)
                     /locus_tag="PLW16_03880"
     CDS             complement(47039..48007)
                     /locus_tag="PLW16_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB70077.1"
                     /translation="MRDVLMEIRGLSKTFHVSGGGLGRGAREVRAVRDVTLSIRAGET
                     LGLVGESGCGKSTLGRLCLALEKPTSGEVRFEGRNIFTLTKPKRKAFRRAVQLVFQDP
                     YASLNPRRTAGQTVMEPLLLHATDPRLVPENTRSIMRTVGLSEEQAQRYPHEFSGGQR
                     QRIGIARAIALKPKLIVADEPVSALDVSIQAQILNLMKQLQRDFGLTYLFITHDFSVV
                     RYMSDRIAVMYLGRIVELASNKAIFANPLHPYTRLLHRAIPVPDPRAKRPGQPAEIDD
                     HPPSEEGCLFYPRCPERLDRCREWHPTLFEPEADHRVACIRKKGSL"
     gene            complement(48084..49058)
                     /locus_tag="PLW16_03885"
     CDS             complement(48084..49058)
                     /locus_tag="PLW16_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70078.1"
                     /translation="MTAPPLLEINDLRTVFDTPAGTVRAVDGVDLVLGRRETLAVVGE
                     SGCGKTVLALSVMRLVPTPPGRIASGSVLFRGENLLALDRKRLQDLRGDRISMIFQEP
                     MTSLNPVFRVGDQVAELILRHRKTTRRQALDEVAGLFHRVGISEADQRLRAYPHQLSG
                     GLRQRIMIAMAVACRPDLILADEPTTALDVTIQSQILALLDQLKREMGTSLILITHDL
                     GVVAQMAERVIVMYAGKVVEEAPAEVMFDTPRHPYTQGLLASLPRLGTDPATPLPIIP
                     GQVPSLYDLPRGCRFQDRCPDVRPVCRQEEPALLTPSPGGTVRCWNYA"
     gene            49191..49646
                     /locus_tag="PLW16_03890"
     CDS             49191..49646
                     /locus_tag="PLW16_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70079.1"
                     /translation="MTKVLFVCNANIVRSFMAERIFNHRLGRSSLSGVSASSAGLLNM
                     NGAPADTTARRILAEHGIDDEGHASRFLTGEILREADLVLTMELPQLRKIESQYPEFA
                     GGLRVLKSYLPDYDIQLGDIADPYRRSVFHYRLCFAEISLSVEAMITCI"
     gene            49637..50431
                     /locus_tag="PLW16_03895"
     CDS             49637..50431
                     /locus_tag="PLW16_03895"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB70080.1"
                     /translation="MYLEYWGLQKFPFENVPDPEFMYYSTEHEEALARLLYVVKRNKG
                     AMLLTGEIGCGKTLLSRVFIRELAESDYDIGLITNPSLIPMDFVREMLFQFGVNTQAT
                     AKSELLTVLNERMMQNVQNNRTMLIIIDEAQLIPMETLEEIRLLLNFQLNDRYLLNLI
                     LIGQPELREKIRGHKQLDQRIFIRYHLNPLNLEDVRNYIAFRLQKAGRPENMFTQEAM
                     DEIFSHSDGLPRKINNICDLSLLIGFSMKYTKLDVEIIRKVIQDSM"
     gene            complement(50508..51707)
                     /locus_tag="PLW16_03900"
     CDS             complement(50508..51707)
                     /locus_tag="PLW16_03900"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB70081.1"
                     /translation="MKTTSSNDTQLRLIQEILFGASPRLPEADDAFWQGFVARCQRER
                     VEGSVYYRLKKKGLDLALPAFWRENLAGRFQRNLIANLTLKHRLKPVLAGFNAAQLDH
                     LLLKGLALAEYLYPSPGMRGMSDVDLLVRKDELLQVNACLEALGYRAVDGDAAVALAN
                     PEGYLASLEYHRADRMPPMLHVHWHPVNTSVPAEAFAPFFDVASLWERAVTVRFDDVE
                     TKILSPEHLLIYLCEHALRVNHTFDRLILVYDLVCLLDTFAEHLDWDAVAREAGAFHL
                     SGLVYFALKQVVHDAGVTLPAGILDRLRPPRITFEQRIFSMLQRRGLRIRGSSYLLYL
                     SMNEGPAKKIHFSLRTLFPPRIIQRQRRGAHDAHRASWLERARVIEIVAHLVRVLPDL
                     LRGRSVD"
BASE COUNT        10155 a        15423 c        15202 g        11011 t
ORIGIN      
        1 gccgtgcgct tcacgaggcg tcgatgcccg atatcgtgat ccttccgatg gccgagctca
       61 agacgaatta ttatttccgt ttctcggcgg tggatcgtcc cggcgtcctt tcaaagatat
      121 cggggattct cggacagaag gatatcagca tcgcggcggt tattcagaag gggcggaagc
      181 gcgacggagc cgtgccggtg gtcatgacga cccacaaagc gcgcgaggcc gatgtccagg
      241 cggccttgca ggtgatcgat cgcctagacg tcgtcatggg aaagacgcag ctttgccgca
      301 tcgaggatga gagcctttaa cgacggcccc ggccggcggt acggtggata cgcatgaagt
      361 acgcagtgtt gcttggcgac ggcatggccg acgagccgct cgacgaactg aacggccgga
      421 cccccctcga ggcggcgcat acaccctaca tggatcgcat tgccgcgcag gggacgctgg
      481 ggctcctcga tacgattccc gacggcttca ccccgggaag cgacgttgcc aatctctccg
      541 ttctcggcta tgacccccgg gaatgctaca ccgggcgcgg ggccatcgag gctgcgaaca
      601 tgggggtgag cctcggtcct agcgacgtgg ccttccgctg caacctggtc acgctctccg
      661 atgacgcctc gcccgtcatg gaggatttca ccgccggtca tatctcgaca aaggaggccg
      721 gatggatcat ccgtgacctt ggggaggcgc tgagcgacgg tcaaggcgac ttttttgccg
      781 gcgtcagtta ccgtcatctc tatgtccgac gcaatggcaa tgaggcgatg cgcaccacgc
      841 cgccccacga tatcgtcgga cagccgatcg accgctacgt tccggccggc aagggggccg
      901 gggcgattct cgagatcgcg gacagggcac gcgggatcct ccgggatcat cccgtcaacc
      961 gagagaggcg caggcagagc cggcgacctg cgaacgccat ctggccctgg gggcagggca
     1021 gggcgccgaa aatcgagaaa atgactgcaa aataccggat ctccggcggt atgatctccg
     1081 ccgttgatct cctgaacggc atgggtatct atgccggtct cgaggtcttt cgcgtcccta
     1141 aggcaacagg ctatatcgat accgattatg aagccaaagc gcggacggcc cttgaaatac
     1201 tggacgggct tgatttcgtt tttctgcacg tcgaggcgcc cgacgaaatg gggcacgagg
     1261 gcaatctgac ggggaagatc cgggccattg aggacttcga cgcaagaatc gtggggccgg
     1321 tgatgaacgg actggaacgg ggcgggccgt ttcgacttct cgttctgagc gatcatccca
     1381 cgccgatccg cctccgcacc catgcggccg atcccacccc ctttgccgtc ctgtcctccc
     1441 tggatgagga aaatcaaagg cgaggggtgg cctacggcga gacggccgcg cgcgcgaacg
     1501 gactgatggt ttcacctggc tacatcctca tggacgcgtt catcagggac tggaggaggt
     1561 tcattgaccg ggcatcgaat tgacatacgt gtgatctatg cggacacgga tgccatggga
     1621 atcgtctatc acgccaacta catcaaatgg tttgagatcg ggcggacgga actgatgcgc
     1681 gcgtgtggaa tcgcccatgc cgacatcgcc ccgctcgggt tcacgttgcc cgtaacgcgc
     1741 gtctattgcc actatctgca gccgtcccgg ctggatcagg tcctggcggt cctaacgacg
     1801 atcgattacg tcaaacgggc cagcgtgaaa tttacctatc agatctggga cgaggctctg
     1861 accgagcgaa gggtggaggg ctataccgtg cacgcctgta cggaccccga cggtcgggtt
     1921 gtccgcctgc cggcgcccct cgtggagcgc ctgcacgctg ttcgatgaaa catcccttcc
     1981 gggatccaag aaagaaggaa gaccatggcc acgaaaatca ataaaaaaga actcaagcaa
     2041 ccggatattc tgcagagcca cttcgcccaa gtcctgccga tgctgcaacg tcaacgaacg
     2101 cgaatcgtac aggccggcgt cgttctcggc gtgatcgttc tcctgggctg cggctgggtt
     2161 ctttacgaat ggagcaacag ccgcgcggcc gcggccctct atctcaaggc ggaggacggg
     2221 acgatgcggg catcgatctc cgacccgtcg aaccgcgaaa aggccctcgc gacctatgag
     2281 aacatcatcg aacagtatcc ccgcagttca agcgcgatgc ttgcccgcta tcgccttgcg
     2341 ggctacgcac acgatcaggg ccagttcgat cgcgccatcg aatattataa cgccttcatt
     2401 aagcaggcgc ccgcggactc cccactcgtc gccctggccg ggaacggtct gggttccgcc
     2461 tacgaggcca agggaaatct cgcgcaggcc cggcagtcgt tcgagcgcgc cttgagctcc
     2521 aaggccggtg acgggttttc ggggatgacg catcaaaacc tggcacgcgt tttcgaggct
     2581 ttgaacaatt ccggccaggc gctcacgcac taccgcaagg ccttggaaca ggtcgacgac
     2641 ccggctttgg cccggcttct caaacggaag atcgccctac tgaattgagg tcatgatatg
     2701 aaaaatagaa aattgatgtc cggcaacgag gccatcgccc ggggcgcata tgaatgcggc
     2761 gtccggtttg cgagtggcta tccgggcacc cccagcacgg aaatcatgga gtcgttcgca
     2821 cgttatccgg gcgtttatgc cgaatggtgc ccgaacgaga aggtcggcct ggagtgcgcc
     2881 atcggtgccg cgctcgccgg gacgaaggcc atggcggtga tgaagcacgt cggtgtcaac
     2941 gtcgccgccg atccgctctt caccgtgagc tacacgggaa ccaacggcgc tctcgtcatc
     3001 atcacggctg acgacccctc cctccacagt tctcagaacg agcaggacaa ccgcaattac
     3061 gccaagttcg ccaagattcc catgctggag cccgccgaca gcgccgaggc gtacgccttc
     3121 atcaagatcg ccttcgcctt gagtgcgcag ttcgacacgc cggtcttcct ccgcaccacg
     3181 acacgcgtat cccattccaa atccatcgtg gagattgccg atcccctccc ggtggaggat
     3241 acgacccaga tccgcttcaa cgcccccaaa tatgtgatgg tcccggccat ggcgcggctg
     3301 cggcggatcg aggttgaaaa acgcatgcag gccttgaggg cctttgccga aacctttccc
     3361 gaaaacaggg ccgagatcaa ccggaccgac gtcggcgtga tcaccgccgg catcccctat
     3421 cattacgcca aggacgtctt tcccgactac tcctatctga agctcggcat ggttcatccg
     3481 atccctgaaa aagcgatccg ggacttcgcg gaccggatcg accgcctgtt cgtgattgag
     3541 gagctcgacc ccttcctcga ggagcagatc cgcgccatgg gcatccccgt caccgggaag
     3601 gacgcctttc cctatacgaa cgaattcgat ccgggggtcg tcgagcgcgc gatgcggacg
     3661 gatcctgtcg aggatcccgc ctatgttcca cctgtcgtgc ctccgcggcc gccgaacctc
     3721 tgcccgggat gcccccatcg agggctcttc tatgtcctgg ggaaattgaa ggtcttcgcg
     3781 gccggagata tcggctgcta taccctttcc ttcatgaaac cactggaagg cctgcacgcc
     3841 tgcatctgca tgggtgccag catcggcatg gcgcacggca tgagcaaggc cttgggagaa
     3901 aaggggaagg ggaaggtcgt cggtgtcatc ggggattcca ccttcatcca ttcgggcatc
     3961 acgccgctgc tcaatgcggc ctacaaccgg agcgactgcg ttttcatcat ctgcgacaac
     4021 cggaccaccg ccatgaccgg gatgcaggaa catccggcca ccgggttcac cctgcaaggc
     4081 cagtcggcca aggccctcga tttcgaacgt ctggccgagg cgcttgggat cgatcaggtc
     4141 cgcgtaatcg atccgtacga catccgtcaa acccgcgcgg tcatccggga ggagctcgcc
     4201 cggagcggac cgtccctgat cgtctcacgc cgggcctgcg cgcttttccg ccgcgcagca
     4261 ggaccgccgc aaaagccgct ccgcgtcgat ccgggcaagt gcatcggctg tcggacctgt
     4321 ctcggattgg gatgcccgcc gatctcctgg cgaaaattct cggaccaggc cccggatcaa
     4381 atcgtgaaaa acacgaaaaa gcaggatggg aacgcggtga tcgatccgca actttgcaac
     4441 gggtgtaccg tgtgtcagca gttatgtaag gccggcgcga tcgttgccgg agaggagtga
     4501 gagaatgagc aaaccgaagg accgtgcgac gggcgaaaaa tccgtccgga gcattctaat
     4561 ggccggtgtg ggagggcagg gcatcctgcg agcaagcgac atcctctgcg aggtcatgat
     4621 acgcaccggc ctcgacgtga agaagagtga agttcatggc atggcgcagc ggggcggctg
     4681 cgtgacaagc cacgtccgct acggaacgtg cgtccattca cccatcgcca agaagggcga
     4741 tgtggggatc ctgatcgcct tcgagaagat ggaggcgctc cgctatctcg attatcttcg
     4801 ggcggagggg atcgcgatcg tcaacgacac cgaaatctat ccccctgccg tcaatctggg
     4861 tgaagcccgt tatcccgacg atatcctcac cctcttgccc ctgcccccag cctctgtgaa
     4921 gatggtcagg gcgcaggaaa tgggagtccg cgccgggaat ccacggacgg aaaatatcgt
     4981 gctgctgggt gtgttgagcc gctatctgac gctcgagccg tccgtgtggg acgaggtcct
     5041 caaggtctcc tttcccgcta agctcgtcga cgccaaccta acggccttcc atctcggaag
     5101 agtggcctga tcgtgtgcac acccgccttc cttgacaaga cgtggacaat gggtatctta
     5161 gcgcggaaga cgttcgctga ctgacggggg aaggggtttg gccgtgtcgg aagactatta
     5221 caagattctg ggtctggaaa aagccgcagc agcggaggag atcaagaagg cctatcgcaa
     5281 actggccctg aagtaccatc cggaccgtaa tccgaacaac aaaaccgccg aggacaagtt
     5341 caagcaggtc agcgaggcct atgccgtcct cagcgatccc gaaaagcgcc agcagtatga
     5401 tcagttcgga tcggccgagc agtttcgcca acggtacagc caagaagaca tcttccggaa
     5461 tttcgacctg aacgacatcc tgcgcggctt cggattcagt ttcggcggcg cgggtggggg
     5521 agggcgggtc tatacaagcg gcggacggag acgcacctac cgccagatgg acggtgatcc
     5581 ctttgcggac cttttcgggc ggggaggcgc accttatgga ggccccaccg tcccgcgggc
     5641 aggacaggat ctggaataca acctttccat tacagtggag gaatcggtct tcggggcaga
     5701 gaaaaaactg gccttgcaga aggaggaccg gtccgaggag atcagcttca agatcccccc
     5761 gggaattgcg aacggaaaga agctgcgcct ggcaggcaaa ggagccccca gccccagcgg
     5821 cggacctccg ggtgatctgt acatgaacat tcagattcag ccgcacccca tcttcacccg
     5881 ggagggcaac gacctttata tcgagaagtc agtccgattt tcgcaagcca ccctgggaac
     5941 ggttcttgat gttccgaccc tggacggggg gacgaaacgg atcaagatcc cggccggaac
     6001 gcagcacaat accaagatca ggatgaaggg atacggcgct ccgggcctca aaggggctgc
     6061 aaagggggat cagtacgtga agatttccgt cgaggtccct aagaaactga ccgacagaca
     6121 gactaagctg atccggcagc tcgacgagga aggcctatag gcctggatgc aacgtctcat
     6181 aagatacatt atgtaaacat tatcatccgg cgagctcaac gtcaccggcc aaccccatgt
     6241 ggctcccgca gatgataacc accccccgga tgccgggtag tatctcctcc gccgtcttca
     6301 atcccctctc gatgtctttg gctgacttta caaggttacc gatgcgcgtt gcggcggcgt
     6361 cagccaaggc ggccgatacg gacagaacgc atacggcgtc ggcgcgtccg taactgaacg
     6421 agtgaccgac ggtcccggag gaggtgcaga cgccgagcgg catccggtcg gaacggatca
     6481 tcaggatgat ctttccactc agggacgatt gacccgcatg gacttctacc cgaagatccc
     6541 tcttgcaatc caggtagata tcccctccat tctcaaccat tacattagag atgcccggta
     6601 gtgttcgtag gcttcgtccg acggattccg cgattgcccc ggccacggcg gccatcggac
     6661 ccacctccgc ccggcgtgcc gcgagcagca tccgcctgac gatgtccgga gcgagcggat
     6721 cttcgggaag gggagttctt gcgtgcagaa attcgggatg atgcaggata taggtttcga
     6781 tctgcgcgcg acaggcatgg acggtctcca gcgtttcgtg cgacagatcc cggtcagcgc
     6841 ggaggaacag gtccgtttca cggacgcata cggaaaaggc ggcaagattc ccctccggtg
     6901 cgatccgatt tcggtaggtc cggaattcgt agcgatggcc aggagcatcg cgaccggatg
     6961 gccgcccttc cctctccgtc tgaccgttca ttggaagatt gtcgccccac gcagaccgat
     7021 cagaccgtcc gcggtctccc gcagtcccgt cgaggcggta acacgtgttt catctccgag
     7081 cgagacgacc gtttccgttc cgttatcgtg aacatgtaga aatccatcgc atttgcccgg
     7141 atagtcgcga atcaggaggt cccgaagaga cagaagcgtc tcgacggtca cacttcgggc
     7201 cgtcatatga aaatggacgg atgaaaaggg ccggtccgtc gcctcctcca ggtgttcgat
     7261 ctctgttgcc atcacgcgga cggtctcttc gccgacatcg attgtgcccc ggaccaggag
     7321 cggatcgtcc ccgtgcagaa ggtcgtatcc cttccggtaa atctcggaga agaaaatgca
     7381 ttcaaaggag cccttcaggt cctctaaggt cacgtaggcc atcgtgtcct tgcggcgggt
     7441 cttgacctcg cgcagggcgt tgacaacccc cgccatggcc accgtctcct tgtcccgcct
     7501 ttcggacagg gagacggagt ccgtgtcggc cagcatggcg aggcgttccg agtacttgga
     7561 cagggggtga cccgtgatgt agaacccaag cgtgtccttc tcgtaagcga ggcgctcttt
     7621 gtgctcccac tcgggcagat cgggaatcgc ttcttccagg gatttcgcgg aagtctcccc
     7681 ggactcgtcc gtgtctgtaa acatgctcgc ttggcgactt tcggcatcgc gctgacggac
     7741 gtggatgcgc tccgtcaatt catcgaggga catcaggagc gggcggcgtt tgcatcccat
     7801 cgcgtcgaag gcgccgcatt tgatgaggct ttcgattaca cgacggttga ccttccgaag
     7861 gtccactcgc gaacaaaaat caaacaggga gcggaattcc cccgtagcgt cccgtgcctg
     7921 gacgatcgtt tcgatcgccc ccatcccgac atgcttcact ccggccagac cgaagcggat
     7981 gtgatctccg gaaacgctga aatctcgtcc agaggcgttg atgtcggggg gcagaacctg
     8041 gatgcccatt tcgcggcaaa ccccgatgta tttgatgatc ttgtcgcggt tgtccttttc
     8101 actcgtcagc agggccgcca tgtattccgc cgggtaatgg gccttgaggt aggccgtctg
     8161 gtaggagatc atggcgtagg cggtgctgtg ggacttgttg aaaccgtatg cggcgaagct
     8221 ctccatctgt tcccagatcc tgatcgcctt ctgctcaggg atctcgtttt gctttgcccc
     8281 ggataggaag cgaggctttt ccttctccat ggccgacacg atcttcttgc tcatcacctt
     8341 ccggagggtg tcggcctggg ccatggtata accgccgatc acgctggcga tctgcatcac
     8401 ctgctcctgg tagagaataa cgccgtacgt ctcgcgcagg atatcctcaa gctctttgac
     8461 ttcataggct atcttcgttt ttccctgctt ccgggagatg aattccggca ccatccccat
     8521 cggcccggga cggtacaggg cgatcagggc gatgacgtcc tcgatgcagt ccggcttcat
     8581 gttgacgagg atatccttca tcccggagct ctcaagctgg aagaccccgt ccgtctcccc
     8641 ccgcatcagg agccgatagg tcttctcgtc attcaagggc aggtccttca tgttcagttc
     8701 gacccccctg ccctcacgga tgaatctcaa cgtattcttg atgacggtca gcgtcttgag
     8761 gcccagaaaa tcgaatttcg tcagcccgac ttcctggaga tcgttcatgg aaaactgggt
     8821 gactacgtca tccttggggc tccggcagag aggaacgcgt tcgaccaggg gcacgtcgga
     8881 aatgacgacc ccggcggcgt gggtcgacga gtgccggttg agcccttcca gggcgagtga
     8941 caactccagg agcttgcgga tgcgctcatc cttttgttgc gcctcctgga gtcgcggttc
     9001 catctggatc gcctcgtcta gcttgatatt aaggatgttc ggaatcagtt ttgcgatccg
     9061 gtcgacctcg ccgtagggga tgctgagggc gcggccgaca tcgcgaacga ccgccttggc
     9121 ctgcatcttt ccgaaggtga tgatctgggc gacgtgatca gcgccgtatt tctcggtgac
     9181 gtaacggatg atgtcgtcgc ggccttcctg gcagaagtcg atatcgatat ccggcatgct
     9241 gatccggtcc ggattgagga agcgctcgaa aaacaggcgg tagcggatgg ggtcgatgct
     9301 ggtgattccg atggcgtagg ccacgagact ccccgccgcg gagccgcgac cgggaccgac
     9361 gggaatgtta tttttcttcg cataattgac gaaatcggcg acgatcagga agtagccggc
     9421 aaaccccatg gttcggatca tggccaattc gtcacggagg cgctcatagt aacgctcgct
     9481 gagcacggca tcttccgcgg gcacgatgtc gggcagcagt gcctccagtc cctcgagggc
     9541 gctgtgctcc agcctctcgt ccagtgtttc gtctgctgcg agagagaaat gggggaggta
     9601 gaatttgccg aagtcgaact gatagttgca acgatcggcg ataacggctg tgtgggcaat
     9661 cgcttcggga cagtcggcga aaagcgcggt catttcatcc ggggagcgaa aatagaaggc
     9721 atccgtcgca aaacgcatcc ggtcggcatc tccgagcgtc ttgcccgtct ggatgcagag
     9781 aaggacctca tgcgcctccg cgtccttcct ttcaagataa tggcagtcgt tcgtggcgac
     9841 caggggcaga tcgaggcggc ggctgagtgc cctgaggcct tcattggcct gcgtctgctc
     9901 gggcagtccg ttctgcatga tctccagata aaaatttccc gcgccgaaga tctggcggta
     9961 ttcttccgcc gccttcgcgg cacctccctc gtcgccgcgg aggattctct cggcgatctc
    10021 tccgtgcaga caggcgctca tcccgatcag gcccttgtga tgtcgggcca gcagctcctt
    10081 gtcgacccgc ggtcggtaat agaatccctc gagaaaggct gcggtcgtca atttcttcag
    10141 gttgtcgtag ccctccgcat tttcgacgag tacgaccaga tgattggaag actcgccgat
    10201 cccttgccct ccttttgtga agcggctttg gggggcgacg taaagctcgc acccgaggat
    10261 gggtttgatg ccggccttaa cggcgcgttg gtaaaaatcg acggcgccgt agatattccc
    10321 gtgatccgtc atcgcgatgg ccgtcattcc gaaatcgtgg gccttgcgga atagatcgtc
    10381 cagacggatg gtgccgtcga gcagactata ctgcgtgtgc agatgaagat gtgtgaagcg
    10441 agcggtatca ctcatggcgt gcgggaccct ctgcatcccc ggagaccgta aagaatttca
    10501 aaggctgcgg gggtgtcaat ccagccagta attgggggcc tccttggtga tgatgacgtc
    10561 gtggacatgg ctctcccgca gacccgccga ggtgatcttg acgaaccgcg cctttttatg
    10621 gaggtccggg agggtttggc agccgacata gcccatcccg gccttgatgc cgccgatgag
    10681 ttgctgaatg cttgccgaca gggagccccg gaatgggacg cggccttcga tgccttccgg
    10741 caccatcttc aggtcgccct ccatgtcatc ctgataataa cggtcgcggc tgcccatttt
    10801 catcgcctcg agcgagccca tcccgcggta gaccttgtag ctccttccct ggaacagaat
    10861 cgtttccccg ggactttcct ccgtcccggc aaagaggctt ccgatcatca cgacatgggc
    10921 gccggcggcc agggccttga cgacgtcacc cgaatatttg atgccgccgt cggcgatcac
    10981 ggggatgccg tgctttgccg ccgcgcgata ggcgtcgcgg atcgcggtca tctggggaac
    11041 gccgacgcca gcgatcacgc gggtcgtaca gatggagccc ggacccacgc cgaccttgac
    11101 ggcgtccacg ccagcctgga tcagactttc cgcgccctcc gcggtagcga catttccagc
    11161 gatcagctca cagcccggga aattcgactt tgtagcgcga acggcttcta tgactccgtg
    11221 agagtgcccg tgggaggtgt cgatcacgat gacgtcggcg ccggccgcga ggagcgcatc
    11281 gacgcggggt tcgcggtcca ggatgccgac ggccgccccc acgcggagac gtcccaggga
    11341 atccttacag gcttggggat atttcttgat cttttcgatg tctttgatcg taatcagccc
    11401 cttcaggcgg tattgatcgt cgaccacaag gagcttttcg atccggtgtt tgtgaaggag
    11461 tttcttggat tcgtccaagg aaatactcga ggtgaccgtt accagatttt ccttcgtcat
    11521 aacgtcggaa accggctggt ccagatcggt ttcaaagcgc aggtcgcgat tggtcaggat
    11581 accgacgagc cgtccgtcct tgaccacagg cacgcctgaa attcgatact tgttcataag
    11641 ctccagggca tggtgaactc gctgctcggg ttcgatggtg atcggatcga cgatcatgcc
    11701 gctttcggat ttcttgacct tgtcgacctc gatggcctgt cgttcaatgc tcatgttgcg
    11761 gtgaatgatg ccgagcccgc cctcctgggc catgcagatc gcggtcgtgg cctccgtgac
    11821 ggtgtccatg gcggcactga tgatgggtat attgagagcg attcgcgggg tgaggcaggt
    11881 cgcggtttca acattccccg gaaggatggc ggattccgcg ggaaccatca ggagatcgtc
    11941 gaacgtcaag gcctcgcgga tgggatcgtc cagcatggat taattctcct tttccctggg
    12001 tgtttgaagg gtgtatcgtt tctgatcaac gtgcaattcg taacgctcgg acgcggcatc
    12061 gtagacaatg cgttccgcga gctgataata ggccgcacgg gaaaagcggg tcttgaaggt
    12121 tccacccttg acacggcagt acatgacgtt gtcgtcgcca atccggatcg tttcggggtc
    12181 gagcggttcc agggtgttgt cagacaggga aagcaaaagg catgcaggcg aggtttctct
    12241 ctccgccggt ccggtaagga ccgcgcggac cacgaagggg gcatcctcga cctctacgta
    12301 gcagctgtct tcgcccatat cgattatata ctctcctcgt tcgtcctgtt tgaggtgctc
    12361 gtaaaagaga ttgacgattt cgcgacggaa catctcctgt ccgcggtaaa accacacccc
    12421 gtccttgtcg attctgatat ctgttctggg tgttgttgtg tccgtctgtg ccatggtttg
    12481 cgaagccttc tagaccctcc ggctgtccaa gatcagcgtg accgggccat cattgacgga
    12541 atcgatcttc atcatggcct gaaattgtcc ggatccgatg agcccaaggc ggtcccgacc
    12601 gatgcggatg aagtactcat agaggaggcg ggctggtgcc ggatcctcgg ccgcgatgaa
    12661 cgaagggcgc cttcccttgc ggcagtcggc aagcagggtg aactgagaaa tgaccatcat
    12721 ctctcccttg atgtcctcca gggaaagatt catcttgcct tcggcgtcgt cgaagaggcg
    12781 aagatggacg attttctgga agaggtattc ggcatccttc tccgcgtccc ccttttcaat
    12841 cccgaggaag acgaggacac ccttgccgat ttcagatacg gtcccgccgt cgaccccgac
    12901 gcgggcgcgt tcgatgcgct ggacgactgc cctcatggtt gtatcctgtc atttgcctgc
    12961 cttactatca ataatcggcg aggacggcaa gttttttatc cgtgccggac gcatgacggc
    13021 attttcgcga ggggatgccc cttaaccaat gatttccgta atgggaatag atataatatc
    13081 aagatatttc atcacatgag gatcatcagg cgaaaacaga tccatgattc tggcatacac
    13141 ctgttccgtc cagaactccc ctgaaacgcc ttgtgttctg ataccgtagc gcaagccctg
    13201 cctgtcggag gggctgaggc ggcccaaaag ggttttcgtc gtccagatcg atccgaagcg
    13261 tgcaaaatcg cacaggcagc gcgcctgttc cacctccgcc ccggagagga agacatcccg
    13321 gttcgagccg gccgcgaacc gggtgaccca ttgagagccg tcgacgacgc cgacattcac
    13381 gaagaggtgg ttcccccatt ttttccttcg tttccacgcc gcattcaaac cttgggcgat
    13441 tcgccgcagc tccaaggaaa agtggatcgc atcgaggaga tagtgatcgt cgtcaccttg
    13501 aataaaaaaa caaagaagtc catcctggga gtatgtagag tgtgtcgtcc gcacacgttg
    13561 agcagcggca tccacgtctg tccgaaaccg tcgaagaaat tcaaaaaact cctccggtgg
    13621 cagctccgcg cgcaattgcg ggtaatcctg gatgcgggcc gaaaggatac agaaggatcg
    13681 ccaatcttgt ggcgaaggtg gtgtgagcgc atcgtcgccc ggagagatgg cgctttgaag
    13741 tccatccgcc tcgaacgttt ccgacgtctc ggcaatttct ggaaaaagct cagtctgaag
    13801 ggcgtggatt gacgtctgtt cttcgcgagg gggggaggtt cgacgggccg ctgaggatgc
    13861 gctagattta gaggcgaaaa taccaacacc gagcgccgta gagatttccg ggatggtttt
    13921 gccgtctttt ttgaggatgt ggaccttctc caaggttgaa aggacatctg acgggaaata
    13981 tccaacttgg cgggcacgca tttccgggtc acgaggcttg ccgacgaccg gcttcggcaa
    14041 cagtccaagt tttatgtaat tattcattgt agctctggaa atccccgtga ttttaagaag
    14101 atcaacgctg ctgatcatat ctgcaaggac cgagttcaca gaggacatgt aactgcctcc
    14161 cctttcatgt attgtccaaa tgagcaatag ctgcttgaca tcttggactc agaaggcgtc
    14221 caagtcaagc agtaaatcgt tagttatttc tgaagtaaat atatttgata atacatgtgg
    14281 atgaactgtt catctttatg tattatatta taattgatca tttcataaca caccctgttt
    14341 tcgtttgcct gggccattct tgtatggtac gttccgccat gcaacggccg aaacacatcc
    14401 tattcgtcac accttggatt tacgactttg ccgccttcga cttctggatg aagcctctgg
    14461 ggctgctctc tctcgctgca ctctttcgtg ataatggtta ttcggttaac tatcttgact
    14521 gtctaatgga tcacgatctc gcggatcagg ctcaccggaa aacaagacgg accgagcacg
    14581 gaacgggaca tttcgataag gaggccatcg taaagcctgc ccccctcgcg ttcttccaga
    14641 gacggtatta tcgctacggc atcccgatgg gcctttttcg tcgaaaactc ctctcgattc
    14701 cccccccgga catggttctc gtcacctcca tgatgaccta ctggtatccc ggcgcatttg
    14761 aggccatcca ccttctccgg gcgtcgcttc ccggtgttcc gatccttctg ggaggcaact
    14821 atgccaccct ctgtcctgat cacgcgcgac aatccggggc cgacaggatt tttccaggtc
    14881 cgggcgaatg gcatttttcg gagatcatta aaaccttctt caatgatcgt cctcgtatcc
    14941 tcccagactt cgacgacctg gacacttacc cgcgtccggc cttcgatctg cttggtcgcc
    15001 tggatcaagt cccccttctg acctcgcgcg gctgcccctt tcgctgctct tactgcgcct
    15061 cgaatcaact gtatcctaaa tttatgcgac gtcatccgtt gagagtcgcc gaggagattt
    15121 tcgactggca cgatcgtttc ggggttcgcc atttttcaat ctatgacgac gccttcctcg
    15181 tggacccccc ttccctcgcc ctgcccctgc tcgacacgat cgtccaacgc aaagcgaatc
    15241 tctcgtttca ctgcccaaac ggcctccacc tccggcagat cacccccgtc ctgtcgagga
    15301 tgctgtaccg ggccggattc cgtacgctca ggttcgggtt tgaaacattc gaccctgatc
    15361 gccaggcgac gaccggtgga aaagtcaaca acgatcatct catcgccgcc gttcggtatc
    15421 tgtgcgaagc gggctatgaa gcttccgata tcggcgtcta cctcctctgc ggtctgcccg
    15481 gccaaaccgc agaggagatc gatggaagca ttcgcttcgt gcagtccctg ggggccaaac
    15541 cctatctggc cgaatattcg cccattcccg gaaccgcgct ctggccggaa gcccttcagt
    15601 cctctccgta tgatcttttg aacgaaccgc tttgccacaa caatacgctg cttccctgcg
    15661 cgggcgatac ccttcccgag ccggcgtaca gggaactcaa actaaaaacc cggatgtccc
    15721 cccgatgaaa ccgcttgatt cttccggcga taaccgttaa acttgtacca ttgatgttga
    15781 cggatcgtga aggatatcca tttcatgagt gcattctcca taggccgctg tatccggcag
    15841 ttcgccctgt ttctgatttc cagcctgttc ttccttttcg gcatctacgt catgatccgg
    15901 acgtaccacc tggaaaatcc cctcgaattt gtcatggcct ttttctcatc ctccctcatc
    15961 attctgatca gcctggtcgg gatgatttcc ccctcggttc aggtctacct cgcctggcgt
    16021 aaaagatgaa cctatgaacc tccagacatt caaacgcctt acggatcgga tcgaggggta
    16081 cgagcccgaa atggttcaga tgcagatcga tctgtgttcc tatccggcgc tttccccgga
    16141 caacggcggg gatggcgaac aggaaaaggc cctctatctt caaggttcca tgcggcgcct
    16201 cggtttgacc gacatcgaga ccctcccggc gccagattca cgcgtgtcgt ccggcctgcg
    16261 tccgaatatc ctcgtgcgtc ttccgggaca ccaaccggat aagaccgtct ggatcctgac
    16321 gcatatggac gtcgtccctc cgggcgaacc ctcccactgg gcgagcgacc cgtatcgcgc
    16381 cgaagtccga aacggccgca tttacgggcg gggaacggag gacaaccagc aggaccttgt
    16441 ggcctctatc ttcgcgctac gcgcctttta tgagttgggg gtcaccccgg aatataacct
    16501 gggtctcgcc ctggtggcgg acgaagaaac ctcaagtcgt ttcggggttc ggcacctgct
    16561 cgaccaagtc aagccggttt tccggaaaaa cgacctgatc gtcgttcccg atttcggcga
    16621 cgccgagggg accgccattg aaatcgccga gaaaagcctc tactggcttc gcttccggac
    16681 gacgggacgc cagtgccacg gcagccgccc cgacctcggg cgcaatgcct tcgctgccgc
    16741 ggctcacctc gtcgttagac tccgggagct ctatgacctc tttccattgc ggaatccgct
    16801 gtatgagcct caggaaagca ccttcgaacc cacgcgcagg gacgccaacg ttccgaacgt
    16861 caatacgatt ccgggcgatg acgtctttta cctggacgcc cgtgttctac ccgaatatcc
    16921 cctgacgcgc gttcttgagg cgatccgggc gcttgcagac gatatcgagc agattcatca
    16981 ggtggggatc gtcatcgaaa ccgtgcagtc cttgccagca ccgcctccga cacccgccga
    17041 tgccccggtc gtcgccgcgc ttcgggaggc cgtccaccgc gtctatggcc tccacgccaa
    17101 gccggtcggc atcggcgcgg gaaccgtggc cgcgttgctg aggcaggagg gatatcccgt
    17161 ggccgtctgg tcacgccaga acgaaaccgc ccaccagatc aacgagaatt gcctcatcag
    17221 caacatgatt ggcaatgcca aggtttttgc ccatttgggg ctgaaccccg tctgaaccct
    17281 ttcggagttc ttgatcatgc aatccttgac ggagaaactc acattcgacg acaacccctc
    17341 cctgaaggtc ctgttgggcg aacgggatgt gcacgtccgc caggtcgagc gcctgacacc
    17401 ggtcaaggtg tccctgcgag ggaatcagct ctccatcgaa ggcggcgccc ccgacgtcga
    17461 attggtccgg tccctcttgc tccagatgta cgctcttgcc cgcaaggggt tcccgatcta
    17521 tccccaggac attcttcacg cccaccgtat cctgtcggaa aaccggcaga cggacctgga
    17581 aaagatcttc ctcgatacgg tcttcatttc ctcgaagaag cgggccatca ctcccaaaag
    17641 catcgctcag aagcgctata tcgacgcgat tcgcgactgc gacatggttt tcggcatcgg
    17701 tccggctggg accggaaaaa cctatctggc catggccatg gccgtcgccg cactacagga
    17761 caagaaggtc caccgcatcg tgctgacacg tccggccgtg gaggcgggcg agaagctcgg
    17821 cttcctgccc ggcgatctgg cggagaaggt gaacccctac ctgcgcccgt tgtatgatgc
    17881 acttttcgac atgatggatt tcgaccgcgc ctcggcgttg atccacaagg ggaccatcga
    17941 ggtcgcgcct ctggcattca tgcggggtcg aaccctcaac gacgccttcg tcatcctcga
    18001 tgaggcccag aacacgactt cggagcagat gaagatgttt ctgacgcgtc tcggtctggg
    18061 ttccaaggcg gtgattaccg gtgacatcac acagatcgat ctccctcagg agatcaaatc
    18121 aggactcgtc gaagccgaaa cgctgctccg aagcgtccag gggatccgcg tcgtcctttt
    18181 ttcggaactg gatgtggtac ggcatccact ggttcaagag gtcatccggg cctaccagcg
    18241 gctgaatgga acgggccctc gcttcaacgg accttgagtc gggttgaggg ggatcacggt
    18301 gaaggcctct aaggggaata gggaggggag acaatgccgg tcctgatcga aagcagacaa
    18361 accgtacgcc gcatcaatcg ggtcagaata cggcggatcc tgacggaggc gctggtcctg
    18421 ctcggctgct ccgatcacga ggtcagcgtg cttctcgtgg acgacatcgg cattcagacc
    18481 atcaaccgtg actatctggg ccgcgatcgg ccgaccaatg tcatgtcgtt cgcgatccgg
    18541 gagggcgaat acggcgctct caatcctcac ctgctcgggg acatcgtcgt ctcggtcgag
    18601 aaggcctggt cggacgcgcg tcaggaaaag atcaagttca cggatgaact ggattttctc
    18661 ctcatccacg ggttgcttca cctgctgggc ttcgatcacg aagatgtccc cgcgacgaaa
    18721 gcgcgggata tgaaggccat ggaacggcgg atctttcgcg atctcaaggg gtacgagatt
    18781 cagtgatagg ccgcgtgcct attccagcac gatcgactgt cccgggtaga tatggccccc
    18841 ctcgccaagc ccgttcattt ccttcagggc cttgagggtc gtgccgtttt tcagggcgat
    18901 tttcgtcagc gtgtcgccct ttttgacgac ataggtcttc gcggcacgtt tggcgattcg
    18961 gaggatctgt cctgtcctga tcgtattgga gcgcaggcgg ttcgtgcgtt tgagatcgct
    19021 cacggatacc ccatagcgcc tggcgacgga agaaagggtc tctccccggc ggatccgatg
    19081 tgtcagggtc ttgcctgtgt cggctgaaga ggcctggatc ttttcgtagc gatagctggg
    19141 aatcggaatg cgaacgatgc gtccggcccg caatcgctta ttcgacgaaa gacgattggc
    19201 ggaacggatg gcgctggcgg aggtccgata ctttctggcc aggctggtca acgtctcccc
    19261 ccgcttgacg cgatgccgga tggtaacggt ttttttggca accaccgtgg gcttcggctc
    19321 ttcccacgcc ggaatctccg cttcgatctt ggcgaacttt tccgccattt ccgtggggac
    19381 cctcagggtg tattcgcgcg atggcgtcgc ccggtggcga agttccgcgt tcagacagac
    19441 cagaagctcc tcgggttttt cgagtcgggc ggcaatgtcg ctcagacgca tcgaacgggt
    19501 cgttttgacg atctcatacg tgctgaggtc ttcgttcgga gagggggatc ccaggtccat
    19561 cccgtatttc tggggatcct tgatgatggc cagcgtggcc agaaagcggg gtacgtagcg
    19621 ggccgtctca tagggcaatt gatggtaaag atcccagaac cggtcaaaat aattcacgtg
    19681 ctgacgtgaa atgacgcgca gcactcgccc ctcgccgcag ttgtaggcgg ccaagacggt
    19741 cagccagtcg ccgaacatgc cgtgcagatc cttgagataa ctgatggccg cacgtgtcga
    19801 tttctcgatg tccatgcgct cgtcgatcca ttcgtcgcgg ttgagttcgt atttgtagcc
    19861 ggtcgaaggg ataaactgcc acagccccag ggcgcgcgcc cgcgacaggg cggatgcctt
    19921 gaagccgctc tccacgagcg gcagccagga cagttcctcg ggaatgcccg ccttcttgag
    19981 ctcccgaacg atcatcggac ggtacttgaa ggaccgctga taggaggaga tgaaaaagtc
    20041 ccgctcgaca ctttgaaaca gacggatctc cttttcaacg tcctcgttga tccgcatggg
    20101 gatttcgctg cgcttcccgg cggtgacgtt ctgcttggag ctgtagaccg caagaatgcg
    20161 ctgggcgatg aggaggcgaa gatcgtcttt ctgtcgagaa atgtccgtat cgccgtcggt
    20221 ttcgatgatc agggcgtagg cctcatcgag caggttctgg gcattttcca ggtccccctt
    20281 ctgccaatgg gcgttagact cctcgaggag cgcgagggcc tcttccatga tatcgcgttc
    20341 ctcgtcttcg atcttttgcg ggtcgttctt atcgtcgttg ccctcagatg caggggcgcc
    20401 gctcttgaga cgggccgctc cggatgggtt tccaatggct tttgcgtccg atgacggcag
    20461 gacgttttcc gagaggatgg cggcttcccc cctggtttcg gtttgtcgca cggcctgggc
    20521 cagtgtgttg ccttcgtccg cgaccgaagg ggtcaccagc gtcaactgca ttgcgatgaa
    20581 caaactcaga cacgaaacaa cacttctccc aacaaacgtc atcatgaaca gctccctcat
    20641 cggccaacgc ttctccccgc cgcaaaacga cgggtcatct gcggcttttg ggcgtgctta
    20701 gcacatttca acggccttgt cttcattttt atccccccag cagataggcc tcgataaagt
    20761 cgtcgatatc tccgtcaagc acgcgattca cgttgcctac ctccaggttc gtccgatggt
    20821 ccttgaccat ccgataggga tgaaggacat acgagcggat ctggcttccc cacgcaatat
    20881 ccttcttgtt tttgttgacg gcgtcctgtt tttcattcct ttccttgatt ttgagttcat
    20941 aaagccgtga tttcagatat ttcatggcca tggaccggtt tttgtgctga gagcgttcgt
    21001 tctggcactg gacgacgatt cccgtcggca gatgcgtgat ccgcaccgcc gaatccgttt
    21061 tattgacgtg ctgtccccct gccccggttg aacggtacgt atcgatccgc aggtcttttt
    21121 catcgatatc gatgacgatc tggtcgtcga tttcgggata gacgaaaatc gatgcgaacg
    21181 aggtatgcct gcgcgcaccg gcgtcgaacg gggaaatccg aaccagccgg tgaatgccgt
    21241 tttcggcctt ggcgtaaccg tatgcatact cgccctccag gatgaaggtc acgctcttga
    21301 tccccgcttc gtcaccggga agactgtcga tcacggtggg ggtgaatttc cgtttctccg
    21361 cccagcggag gtacatcctc aggagcattt cagcccagtc ctgggcctcc gtcccccccg
    21421 caccagcgtg gatggaaacg atggcgttca gggtgtcttc ctcgtggccc agcattcgct
    21481 tcagttcgtc ctgacgaacg tccttatcca ggcgctccaa ttcggtgtcg atttcggaca
    21541 ggaccgcggc gtcctgttcc gtttcggcta aagagtacag gtctgcgagt tcgtcgagcg
    21601 cgcgttgctg acggtcccag cgttcgacgg tttcgtcgag ggtggttttc tctttgagga
    21661 tttcgcgggc tttttccgga tcgttccaga aatcggccct ctgggacgcc agctccagct
    21721 cccggcggcg gaggttcagg gtggggacgt caaagacact ctccgatgcg cgtgattttg
    21781 tccctcaagc cttccagctt aaatgcggtc tcgctcatgt ttcctcctca tccaggcgac
    21841 gatggtcatg cccaggagca cgataatgct actatacgca aaaaagtcgc catatgtcga
    21901 ataaattgtc ttcttctgga aaagcctgac ctcaccggaa aggccgctcc gctggaagat
    21961 ccccgttttc ttggtgatct taccggtggg cgagatgaag gcgctgatgc cggtattggc
    22021 ggcccggcag agatacatgc gcgtttcgac ggcgcggaag accgtcatgg acagatgctg
    22081 atagggggcc gaggtcggtc caaaccaggc gtcgttggtg atgttgacga gcatttccgc
    22141 accacggcgc ttgtagtctg tcccggctgc ggggaaaatg ccttcatagc agatcaggat
    22201 cccgagccga tggccgttca tgggtaaggg aacatagccc cggccggggc ggaaatcccc
    22261 aacgccgacg gccagtttgc tgataaatgg gaaatagcga cgcaacggca cgtattcacc
    22321 gtagggaacg agatgcacct tgtcgtaccg gccggcgatt cgaccggcgg ggtcgacgag
    22381 gaacgcgctg tttgcgaaat tggtcccaag ggcccggtcg gcataggtcg ggctacccag
    22441 aatcatccag acgcggtgat cgcgtgcgaa ggccagaatc cgtcgctgga gcgggccgtc
    22501 gtcctggaag tagaaaggaa cggccgtctc cggccagacg atcacgcctc cggcggctgg
    22561 gagatgacgc gcggactggg acagataggc atcgaccgta tttctctgat agcgaggatt
    22621 ccatttgaga ttctggtcga tgttcccttg gatgaggctg acggggatgg aggccgattt
    22681 ggcaattccg ttccggacga cgcccagacg ccaaaggccg tatcccgcaa gcagtgagaa
    22741 gacaacggcg atcgcgacga tggaagcgaa gcgcatcgat gcgagtttcg tcgtcaggac
    22801 ttgaaaaaga agcatattga caaggacgat caggaatgtc agaccggcag gcccggtgac
    22861 gtcgacgatt tgaatcagtg ggagacagcg atactgggaa taggctagcg cctcccaagg
    22921 gaaaccggtg aagatccatc cccggatgaa ttcgagtccg gtccagagaa ggggcaccgc
    22981 gatcagtggg gcgcaatggc gaaaaaaaac gcatcccgcc gtaaaaaagg ctgtgtagag
    23041 gctcaggtaa gcgcagagga gcagcatggc cgcaaccccc agcggcggcg agagcgagcc
    23101 gtatttgacg acgacatagg taatccagta gaggatgccg acatgaaaga ccaggccggc
    23161 ggtaaagccg agaatcatcg cctcgcgggt gccttttccg cggagggaga tgatcagggg
    23221 aaccaatgcc acccaggcga tgaccccaca tccgaacttc ggaaaggaaa gaaaaagcag
    23281 gacgcccgtc aggacggcac cgaacagcgg gcccgggttg aaggcgctcg gggtgccgga
    23341 gacggtgtta tcggctttca ctttttcgaa tgcggacctt tttgatgctg cgttcatcgg
    23401 cggcctcgat cttcatctcc agcggcccga tgcgaacgat ctccccgggg atggggatcc
    23461 gcttgaggac atggaggatg agcccgccca aggtctcgta ttccccgtcg tcgaacgcga
    23521 gaccgtaatg ggcctcgagc acctcgatct cgaggcgggc gttcgcaagc acggaaccat
    23581 ccgccagttc gatcagctcg tctccgtttt gatcgtgctc gtcatggatt tcccccacaa
    23641 tctcctcgat caggtcttcg agtgtcagca agcctgaggt cccgccgtat tcgtcgatga
    23701 cgatcgccag atggtatttg ttctgcttga gttcgtgcag cagctgatgc gtgtttttcg
    23761 tctcggggat atagtagggt ttcctcaaga tcgaaagcac atcggcttcc gtcacattcc
    23821 gtgaccagaa cttgtggaga tccttgacgt tgatgatccc cacgatgttg tcgatactgc
    23881 ccttatagac gggcatgcgc gtatgcccgt gcttctggag cagcgccaga atctcgccga
    23941 tggaggcggt tgtgctgaag gcgacaatct ccgtgcgggg gatcatgatt tcgcggacca
    24001 gggtttcacg aaagtcgagg atgctctgaa tcatttcccc ggattgggga tcgagcagac
    24061 cctcctcctc accgctggcg atgatcgact gaagttcctt ttcgaggcgg ccgtggtcct
    24121 tggcaggcac gatctcccga aaaaatgcga tgagcttctg tgcgaagatg gttaacctcc
    24181 cccgttctaa gggtttaaag ggcgcggagt gattccggcc tcaacaggcc ccggtcaccc
    24241 cgctggacga cctcctgaat catgcggacc gtctcttcct tttcttgcgg caggcgcgcc
    24301 cgaatcggaa ggtaattgga ggcatgattc gaggtgaaga agcagtccgt gaactgagac
    24361 cgggcaatca tcgtccccag ttcggtcaaa aagccgaagg agtccggaag ctgaaatcga
    24421 cccgcctgca tgtcctcgta aagcggagtt ccaggaacca ccatgacggt gagcgctccg
    24481 gtgaaatccg gatcgatccg ggtcaggatt tttgccgttt ccgtcgcatg ctcgatgctg
    24541 tgcgctctcc ctccgattcc aagcaggacc gtgacggaca ggaggatgcc cgagtccttg
    24601 atccgtctgc ccgcatcgac catctgctcc actccgaccc ccttgtggat cttttcgagc
    24661 agggcggcac ttcccgtctc cacgccgaga taaaccatct tcaggccggc ctctttcagg
    24721 gccagaagat ctttcgccgt tttgcggagg atgcttttgg cattggcata ggtcgacacg
    24781 cgttcgattc ccggcaggtg ccggcggatc agggccagaa tatcgagcag gcggggctgc
    24841 ggtatgatca gggcgtcccc gtcgcacagg aaaacgcgct tcacccgccg gaagtgagag
    24901 gcctcttcaa ggtcttcgag gatttcctca cgggacttga tgcggaactt cttgtcccga
    24961 taggtcgggc aaaaggtgca tttgttgtgc gagcatcccg tcgtgacctg cagaagcaga
    25021 ctgtaggcct cgctgggagg gcggataatc aatccttcgt acttcatggc aaacgctcat
    25081 tcaaggtttt tgtctcgaat tatacgtgcg aaaagcccac gtgacccgaa aaaaagaggg
    25141 gctgccttga cggccaaccc ctcgttcttc ttggcggggc cgaagggact cgaaccctcg
    25201 ccctccggcg tgacaggccg gcgttataac cagcttaact acgaccccgt ttttgaactt
    25261 aaagaccgtg cttaccgctt gtgtacaaat taccatatcc ccactgggaa aagcaacgcc
    25321 tttgtgacgg acttggtagg cggaacagga ctcgaacctg tgacatccac ggtgtaagcg
    25381 tggcgctctc ccagctgagc tacccgcccc cggcggcatc ctctcctcgg tggcagcgct
    25441 acgtaacagt aaaccctcgt cctgtcaagc gataattgcc tgcgctatga ggccgctttc
    25501 tgagaaacgg gggccaagac agcggatggc cgttcgtctc ggacccccgg cacgatgcgg
    25561 acttcggtac tcacgtcgcc ggtcggctcc actttgggca cgagggcact cgcgagcaca
    25621 tcgtcgatat gctccaccag gacaacattc aacgccttca ggatattggg agggacatcg
    25681 gccagatcct tttcgttgtc cttggggatc aggacggtct tgatgtttcc ccgatgcgcc
    25741 gccagaagtt tttccttcaa cccgccgatg ggcaggacgc ggcctcgcag ggtgatctcg
    25801 cccgtcatgg ccaaatcccc gcggaccttc tgtttggtga acgccgaggc aatggaggtc
    25861 accatcgcca caccggccga ggggccgtct ttcggaatcg caccctccgg aacgtgaacg
    25921 tgaacgtccg tttccttgta aaaattctcg tcgagataaa attggtccga acgggagcgg
    25981 acgtaggtca gggcggcctg aaccgactcc tgcatgacgt cccccagttt tccagtcatg
    26041 gtgatcttgc ccgagccgcg cagcgtggtg gcctcgatga tcaggagttc accgccgact
    26101 tcggtccagg caaggccgac cgtcgtaccg atgcggtcct ggccttccgt ttccccatgc
    26161 cgatactgtg ggattccgag atatttatcg agcgatttcg aggtgataga aatcttcttc
    26221 acgctcacgt ccgtgacgat ttcgcgggct accttgcggc agatcgacgc gatctctcgc
    26281 tccagattcc ggacacccgc ctcgcgggtg tacttgcgga tgatcgtgag gagggcgccg
    26341 tcggaaaagg agatctgatt ctctcgcagg ccgttggcct cccgctcctt ggcgatgagg
    26401 aatttcttgg cgatgcggag tttttccatt tcggtgtagc cggccagacg aataacttcc
    26461 atccggtctt gaaggggcgc ggggatgctc tgcaggacat tggccgttgt gatgaacatg
    26521 acgtctgaaa gatcgtagtc cacctcgaga tagttgtcgt tgaaggcgtt gttctgctcc
    26581 gggtcgagaa cctcgagcag ggccgacgag gggtctccgc gaaaatcgga gctgagcttg
    26641 tcgacctcgt caaggcagag caccggattg ttcgaccccg ccttcttcat gagttgaatg
    26701 atcttcccgg gcagagcccc gatgtacgtt cgtcggtggc cgcggatttc cgcctcgtct
    26761 cgaacgcctc ccagggaaaa ccggacgaac ttgcgattag ttgcgcgcgc gaccgatttg
    26821 gcgatcgacg tcttcccgac gccgggggga cccacgagac acaggatcga tcccttgttc
    26881 ttctgtacaa gcgtctgcac cgcgagatac tccaggatcc gctccttgac tttcttgagt
    26941 ccgtaatgat cctcttcaag gatcgtttcg gattccttta gcgtgtaatg attctccgtc
    27001 ttttcacccc agggcaggtc cagcagccag tcaatgtaat ttcggacaac ggtcgcctcg
    27061 gcggacatcg gggccatcat ctggagtttc ttcagctccg atttgacctt tttgtgagcc
    27121 tcctcggaaa gctttttctg cttgagcttc ttttcgagct ccgcgatctc gttcttgaag
    27181 tcgtccttct cgcccatttc cttctggatg gcgcgcatct gttcgttcag atagtaatcc
    27241 ttctgggtct tctccatctg ctttttgacg cggcgcttga tcttctcctc gacctcgagg
    27301 atttcgatct ccgccaacat catctcgtag atggtctcca gtcggtcggt cacgttgaaa
    27361 atctctagga tcttctgctt gtcctccagc ttgacgttga gatgggaaac gacgatatcc
    27421 gcaagtttgg acggctcgtc gatggaggcg atcgtcccga tgatctccat atggatcttc
    27481 ttgctgagct ttgcgtacgt ctcgaacgcc tcgcggatgc tgcggatgag ggcctccgcc
    27541 cggacgctgt ttggatcgtc gacatccggg atctcctcca cggccacgaa gaagaagtca
    27601 tcggaaggca gatattcgcg aacgccggcc cgctgcttgc cttccacaag caccttgact
    27661 gtgccgtcgg gaagtctgag gagttggatg atgatgccca gggtgccgac ccgatagatg
    27721 tcattctcgg tcggctcatc cttctgagcg ttcttctggg ccaccatgaa gatccccttc
    27781 tcgaacttca tggcggactc cagggcagcg atcgactttt cgcgccctac gaagagcgga
    27841 acgatcatat gggggaaaac gaccacgtcc cgcaggggaa ggaggggaac cttcaacagg
    27901 gaactgtctt cccgatgttt catggtatca ctcatgtgcg tatctttatc cgttcggaat
    27961 cggatcggcg ttcaggcgga ttccgatttg ttttcaaata acaggatggg ctgagcaccg
    28021 ctcagcacca catcctcgtt aatcacacat tcacggacat ccaaccggga gggtatgtcg
    28081 tacatgatgt ccagcatcgt gttttcgatg acggcccgca atccccgtgc gcctgatttc
    28141 cggccggccg aaaggttggc gatcgcacgc agcgccccat cggtgaactt gagtttgacc
    28201 ttttccatct cgaacatctt ctggtactga cggataatcg cgtctttcgg ttcgaccagg
    28261 atccggacca gatcctcctc catcagttcg ttgagtgtcg ccacgaccgg aagtcgtccg
    28321 atgaattccg gaatcaggcc gtacttcagc agatcctcgg gttggacgac tgtcagcagt
    28381 tcgccgatac tctgagattt cttgctccgt atatccgccc cgaaccccag ggtgtttacg
    28441 ccggtacgct tggcgatgat attttcgagg ctgttgaagg cacctccgca gataaagagg
    28501 atattgctgg tatcgacctg gatgaattcc tgctgaggat gcttgcggcc gcccttcggc
    28561 gggatgttgg ccatggtccc ttcgatgatt ttcaggaggg cctgttgcac gccttcaccc
    28621 gaaacatcgc gcgtgatgga aggatttccg gtcttcttgg cgatcttgtc gatctcgtcg
    28681 atgtagacga tccctttcga cgcgcgcgcc acatcgtaat ccgagttctg caggaggctc
    28741 agaatgatgt tttcgacgtc ctcgccgacg tatccggcct cggtcaacgt cgtcgcatcg
    28801 gcgatggtga aggggacgtt caacattctg gcgagcgttt tggcgagcag ggtctttccg
    28861 gaccccgtcg ggccgatgag cagaacattg ctcttttcga tctccacccc ctccatgtcc
    28921 acatcggtga acagccgccg gtaatggttg tagacggcga cggagaggac cttcttcgtg
    28981 cgatcctgtc cgatgacgta ctgatccaga aatcgcttga tgtccgcggg ctcgggaatt
    29041 ctctgaccgg cctgatccac gaccgacggg gacgcgtctt catccacgat gcacttgcac
    29101 agctcgatgc attcatcgca gatatacgcc gacgggcccg cgaccagctt tttgacctcg
    29161 ttctgattct ttccgcaaaa cgaacagtac agcatgccct gtccctcctg ctgtgttcgt
    29221 ccctttttcg tcatggatcc aacctcacgg ttcctatcgg gctaagccct tttggcgatg
    29281 atctcgtcga tgatgccgta ctgtttggcc tgttccggcg tcatgaaata gtcccgctcc
    29341 gtatccgtca tgatcttgct caactcctgt ccggtgaggg aagagaggat tatgttgagt
    29401 tcctccttca gacgcaggat ttctcgggcc tggatatcga tgtccgtggc ctggccctgg
    29461 aagccgccga gcggctggtg aatcaagatc cgggtatgcg gcagggcgta tcgtttgccc
    29521 ttctgtccgg ccgccagcaa cagtgcggcc atgctggccg cctgccccat gcagacggtg
    29581 cacacggatg gcttgatgta ctgcatcgta tcgtagatgg caagtccgga actgacatgg
    29641 cccccggggg aattcaggta aaagtagatt tccttgtccg gatcttctga ttcaagatac
    29701 aggagctgag cgatgatcag attggcgacg tcgtcgtcga tcgctgttcc cagaaaaatg
    29761 atacggtctt taagcaggcg cgaatagatg tcgaacgccc tctccccgcg cccgtcctgt
    29821 tcgatgacca tgggaataag cggcatttat cggtcctccc cttccgacgc ggcggcattc
    29881 cggatcgtca ccgtcgcctt ttcctcgacg aaccggaatg ttttgcgctg gagcaggtcc
    29941 atgcgcacct gttccagact gccctcttct gccagtttcg accgcaggtc cgggatgtcc
    30001 tttcccaatt tggcggccat ttcgtgaagc tgggcatcga gatcctcttc ggtgacggtc
    30061 aacccctccc tgtccgcgat ggcccgcatg agaaacatgg tcttgacgat ttttcccgcc
    30121 tgctccttca gtttttcatg catgccgaag ctcagttccg ccgcctgctg cttgtccatg
    30181 ccagcccacg tcatccggcg ctgcatatcc gtcatcaggt agaacgtctg acgctctaca
    30241 agggacatcg gcacttcgaa cggattctcc ttcagcaggg catccaccat cacctcctgg
    30301 aacgccgcct cgtcccgtcc cttctgatcc ccttccagag aggaccggac atccgccatc
    30361 aaggcttcga gggagtcgta ctgttcaaag ttcttgacga attcctcatc gaggggcggg
    30421 agtttctttt cccgaatccc cttgagactg acgctgaacc ggacatcctt gcccgcaagc
    30481 tgcgcctggc catagtcatc cggaaactta acctgaatgt cccgggtttc gcccttcttc
    30541 atcccgacga gctgctcctc gaacccgggg atgaaggtat gggacccgac ctccagaaaa
    30601 tagttttcgg acttgagttc atcgagggcc tgaccatcga tggtgccgga gaaatcgatc
    30661 gttgcgaaat gtttttcctg gagttcccga tcgtcagaga tttcttcgag ggtgccgtac
    30721 atctcccgga tctgaagcag acgagcctcc aggtccgcat cggtgacgtc cgtctggatc
    30781 ttttcgagtt caagccccgc atagttcttc ggctcgacca ccggttcgac ttcgaccgtg
    30841 gctgtgaaga ggagcgcctg ttccggctcg atggatttgg ggtcgacctc cggctggctg
    30901 accggcgtca agcgctcgcg ctcgatggct tctcgatagt ggcgattgac cagattctgt
    30961 accgtttctc cttcggcgta ttccttgtaa tgggtcttga gcaccggcag tggcactttt
    31021 ccggggcgga accccttgat cttggccttc tttccgacgt cacggtagac ctgatccaac
    31081 tccttcttga cattctccca ggggatgaca aacgaaaact tcttcttgac aggggtgatt
    31141 tcctcgactt ttacatccag tatctgttct tccacgactg ttcgactcct tatagagcta
    31201 aaaggttctc cgcgcccgtg atgttgactg gtgcgagaga ggggacttga acccctatcc
    31261 gcaatgcggg ctggatccta agtccagtgc gtctgccagt tccgccactc tcgccgggat
    31321 accgctttat atatgcattc cctccggcga ttgtcaacgt tattgttccg tgtcccgcga
    31381 ccggaacctc cacaaaaaaa ccgccctacg gcggttttaa tggtcggggc gagaggattt
    31441 gaacctccga ccctctgaac cccattcaga tacgctacca gactgcgcca cgccccgact
    31501 tgtcccgttt tcgcgaaacg gcgtgattcc tatcaccatc aggggttggt gtcaaggaca
    31561 atcgcggcac ataattcttg aaaaaagggg gattgtcctg taaaggtcgg actcagtcat
    31621 acccatgaac cctatgagat cacggggcta ttgaatttga aacgaaacga caccccttgg
    31681 cgatggatcg gagccgccat gctcgccgcc tgtctctcgg ccggatcccc agccgttgcg
    31741 gatatctaca agtacgtcga cccggacggg atgattcatc tcaccaacgt gcctaccgac
    31801 gaccgtgtcc gttacgaatt gctcatccgg gaaaagcgtg tgctcttccg accgggaacg
    31861 gacatcaaga aatacgacca catcatcgat cgggcgtcgg aaaaataccg cgtcgatccg
    31921 gccctgatca aggccgtcat caaggcggaa tccaacttca accaccgggc cgtttccccc
    31981 gtcggcgccc gcgggctcat gcaactgatg ccgaagacgg cttcatccct caatgtggcc
    32041 gattcgtttc atccggagaa caacatcgag ggtggcgttc gctacctccg ttacctgctc
    32101 aacctcttca acggaaatct gcctctggcc gtggcggcct acaacgccgg agaaggggct
    32161 gtcagccgct atcaaaacac catccccccc tatccagaaa cccagaccta cgtccggcgc
    32221 gttctcgatt tctaccgcca atatcgtcaa atcaactgaa acccgcctgg tgggcaagaa
    32281 ctcaatcggc cgtcaggtag ggccgcagtc cttccagtcg cttgcccttg attcccggaa
    32341 cggcggccaa ctcttcgagg gcgtgaaata ctttccgccg gtcgcgttct cgaacgatca
    32401 ggaacgcggt tttcggaccg attcccggaa cgagcgtcaa ctcctccacg gaggccgtgt
    32461 tgacatcgag cgggatgccg agggtcatgc gctctgccgc gggcatttga ctcaaccgaa
    32521 tccaacgttt tccgtcaggt gcggtgacga cttggacgag atcgccgcgg ttgaggggcc
    32581 ggaggagccc cgccgggagg gatatcctgt gcgggtcgtt gtcccactca ccactccgct
    32641 tcaggacgtc gaaggcggtg gatccagcag gaacaaagta gacgccgtga cgacgggcat
    32701 cccccgcgag acggatctcg agggaagacg aacgcgtttc agggctggga tcggctgtct
    32761 tcttttcccc tgaagtcaac gccaccgccc ctaggatcga caggagacag acaacgagga
    32821 gggtgattcc acccgtctgt tgccggccgg tcagcatggc tgaacctaat tccttttctg
    32881 ttccaggtca aaggtgtcgt ggagaaccat cacggccaat tcggtatatt tagcctgaac
    32941 gacgcaggag atcttgatct cggaggtgct gatcatgagg atattgatgc cctcctgcgc
    33001 cagtgccgtg aacatccgcg aggcgacgcc ggagtggctg accatgccga caccgatgat
    33061 ggagaccttg gcgacattgt cgtccagatc aacgttggcg gcgccgatct cccggcagac
    33121 gtcctgaagc aaccggtagg cctcccggct gtcctttttc gagaccgtga acgtcagatc
    33181 ggtaaaccct tccagactgg cattctgaat aatcatgtcc accacgatat tctgcgcgga
    33241 caggggtgtg aaaatcttcc cggcgattcc cggtcggtcc ggcacatgga ccacggtcag
    33301 cttggcctga tcgcgatcgt aggtcacacc ggaaaccact tccttttcca tgtctttgtc
    33361 ctccttggtc actaaggttc ccccctcgtc ggaaaacgag catttgacac agatgggaac
    33421 gccatgtttt tttgccaact cgacagatct cggctgcagg accttggccc ccgtgcgggc
    33481 catttcgagc atctcatcgt aggagatgcg gtcgatccgc cgcgccttcg aacagagatt
    33541 cgggtcggtc gtgtagacgc cgtcgacgtc tgtataaatg tcgcagacgt ccgccttcag
    33601 ggcggccgcc agtgccacgg cgcttgtatc ggaccctccg cgccccaagg tcgtgatatc
    33661 gcctctctca tcgacgccct ggaatcccgc caccacgcag ataatcccgt cacgcagggc
    33721 ctgcgcgatt cctgccgtct ccacatcgag gattcttgcc ttcgcgtgtg ctgagtcggt
    33781 acgaatcctc acctggaacc cgaggaagga tttcgccgga tggttcatct cgttcagaat
    33841 catggacagt agggtgaccg agacctgctc gcccgttgca atgagggcgt cgtactcccg
    33901 actcggagga tttgggcagg ccgactgcgc cagtcggatc agcctgtccg tttctccggc
    33961 catggccgac aggacgacga ccacctgatt ccccatctcc cggacccgga cggcccgacg
    34021 ggcaacgtta cggatttttt cgatgtcggc tacggatgtg ccgccatatt tttgaacaat
    34081 caaagacatt tacctacttt gtgccccctt tccagatatc atcgactctg cgaacaatcg
    34141 ccgccgccgc ccgaagaccg atgtgtcgtc ggcccgcatc aatatagcgc aattcaataa
    34201 aaatacaacc ctcgtcgagg atatcggaga tcttctcggc ccattcgacg accgtaaccc
    34261 cgtcggcgaa aaagaactcc tcgtaaccaa gggtctccag atctgcggct ccgcccagac
    34321 gataaaggtc gacgtggtag agcgtcaatc gtccgggata ttcgttgaca agggtgaagg
    34381 tcggacttgt gatcgcaaag gtttccggga tgtcgagacc gcgcgcaatt ccctgtgcca
    34441 acgttgtctt gccggcgccc aggtcgccac tcagggccac gatcgatccg gcggtcagcg
    34501 tccggccgat cgcctcaccc aggcgacgtg tctcctccgg gcttgggcac accacatcga
    34561 gccgttcatt cataggggtc ggcatccaat tctggtccac gcgggcgacg accgtcggct
    34621 tcgagaacga cgaccgccat ggcatggtcc ttcgtgtgcg tgatcgtgag atgggttcgc
    34681 ccgattccgc gcctttcgag aatccacaac gcatttgcat gcaggaggag cgtagggcgc
    34741 ccgtcccttt cattgaccgt ttcgatctcg tgcaggcgca cgcccatgcc cagaccgagt
    34801 cccaaggact tcaagagggc ctccttgacc gcgaatctgg ctgcgaaatg gattgcaggc
    34861 cgggcgcgtt ccgaacaggc gtcaatctca cgaagggtga acacccgtct gtgaaacgcg
    34921 tccccccacc gtgtggcgat gcgctcgatc ctggcaatct cgacaaggtc gatcccgatc
    34981 ccggcgatca tgtccgcgcc tccttcagtg cgtcgatcat cgacacgacc ttcctggccg
    35041 ccggaacgtt gtggacgcgc acgatcgcgg caccgttcat cgcggccgcc gcgatcgccg
    35101 cagcggttcc ttcggaggaa gccgacagcg tctccccggt gatctgtcgg acgaacgatt
    35161 tcctcgagac tcctgccagg atcggccggc cgagcatgcg cagctccgcc agcccgttca
    35221 aaagtgcaag gttgtccccc agggactttc caaagccgaa gccggggtcg acgatgatcc
    35281 gatcgaagcc gatcccggaa gtctgcgcgg cttcaaggcg ttccttcagg tagaggacga
    35341 tgtcgccggg cagggagcgg tactcggaga gggctgcctg catggtcttg gggagacccc
    35401 gcatatgcat gaggaccacc gccgcacccg attccgcaac gactgccggc atcgccggat
    35461 cgtaggtcat cgcgcttatg tcgttgacga tctccgctcc ggccgcgaga gcatgacggg
    35521 cgatttccgc cttggtggtg tcgacggaca ccggaacctc cacgcatgca tggagggcct
    35581 cgattaccgg caagatgcgc gccagttcga cgtcggccgg aacgggatcc gcgcccgggc
    35641 ggctggattc gcccccgacg tcgatgaagt ccgcaccctc ctcggccatc cggtatccgt
    35701 attgaacggc cgcatccacc gtgggaaatt gcccgccgtc cgaaaaggag tccggagtga
    35761 cattgatgat gcccatgacg gcggtcgttt tcccgacggt catctccctg tggcctgtcc
    35821 gcaggaggta gtcggtgcat tccgagttct cgagaatctc gatcagactc cgcgagagtc
    35881 cggaaaggcc aaagggttga accgcgagct ttgcggccag gcggcgaatc tgcttgggcg
    35941 ttcccatgat cagcacgtcg gtggacgggt tcgtgcaggc gacactttcg cgatgtacag
    36001 cggcatcccc gccggaggcg agcatttctt gcttgaggat gttcgccacg cggcactcga
    36061 catcgtcaag ggcgatcagc aggcttcgca ttttgggaag catggcacga atcccgtgcg
    36121 gatcgacgcc gatccgcgcg agaatcctgc cggcctggag ttctgtgggg gcgtggatga
    36181 gacgggggga atatctcccg tttggcttca gttccatagg cggggctcag gcagggattg
    36241 cggcggctcc tccggccgtg tcgtccgcgt cgggcggaaa gacgagggca tcgatctcct
    36301 ccgcgttcag aacctctttc tccagaagag cgctggccat gcggttgaga atgtccatgt
    36361 gatccatcaa tagcgttttg gatttttcat aggcgtcgga gacgatgcgc gtgatttcct
    36421 ggtcgatctt ctcggcggtc tgttcactgt aatccttgtg tgtagcaaat tcacggccga
    36481 ggaagatctg ttcctccttc ttgccgtaac tcaatggacc catcacttca ctcatccccc
    36541 attcgcagac catcttcctc gcgagatggg tggcacgctc gatgtcgttt ccggcgccgg
    36601 tggtgaaatc attcagcacc agggcctccg cggctcgacc cccaagcagg atcgcgatgt
    36661 tgttcaggag gtacttccgg ggataggtgt gtttttcgtc gattggaagc tgctgggtca
    36721 atccgagggc ccgacctctt gggatgatgg taaccttgtg gatgggatcg gtgccgggca
    36781 gcagcttggc gaccagcgtg tgaccggcct catggtaggc cgtgttgcgt ttctcgctgt
    36841 cgctgatgac catgctcttg cgctccgccc ccatgaggac cttgtctttg gcgaattcga
    36901 aatcgcgcat gccgaccctt tccttcccca tgcgcgcggc gtacagcacc gactcgttga
    36961 cgagattctc gatatcggct ccggagaatc cgggcgtacc ccgtgcgatg acggcaaagt
    37021 tcacgtcttc gtctaggatg gttttcttgg catgcacctg gaggatcttc tcgcggcccc
    37081 tgacgtcggg gagtggcacc atgacctgcc ggtcgaagcg ccccggtctc agcagcgccg
    37141 gatccaggac gtccggtcgg tttgtcgccg acaccaggat gaccccttcg ttggattcga
    37201 atccgtccat ctccacaagg agctggttca gcgtctgctc ccgttcgtcg tgcccgccac
    37261 ccaggccggc cccgcggtga cgaccgaccg cgtcgatttc gtcgatgaag atgatgcagg
    37321 gcgcctgctt tttcccctgg gtgaagagat cgcgcacccg ggaggcgccg acaccgacga
    37381 acatctcgac gaaatccgat ccactgatgc tgagaaacgg cacgtcggcc tcccccgcaa
    37441 tggcccgcgc gaggagcgtt ttgccggttc cgggctgtcc gaccagaagc agacccttgg
    37501 gaattctccc ccccagtcgc gtgaatttct tgggatcctt cagaaaatcg atgacctcct
    37561 ggagctccgc cttggcctcg tcgatgccgg cgacgtccgc gaacgtgact ttcttcgatt
    37621 tgtcggtcat gagtttcgcg cggcttttgc cgaaggccat ggccttccca ccgccggact
    37681 gcatctgccg catgaaaaag atccagacgc cgatgagcag gagcatgggg aaccaggaga
    37741 tgaggatcgt catgtaccag ggggaatcct cgacgggttt cgccgcgatc ctcacgccct
    37801 tgctcctcaa gagcgggatg agatcgtcat ccttgggggc gtaggtctta aacgtcttgc
    37861 ctgtggtcag ctttcccgtg atgttttcac cctggacctg cacttcgccg atctgtccgc
    37921 gctccacatg ggtcagaaag tcgctgtaga cgatgttccc ctgcgtcgcc ttcggctcgt
    37981 tgaaaaggtg aaagagcagg acgaagacca ggctgataac cagccacaag gcaatatttt
    38041 tctgtaaggg attcaacgat caggctcctg ttgtctactt cgcgcttcga cgtgcagggg
    38101 cacgtcggtt tacatctccc gtacggaccg gtcttacgga tccggccttc gaatgggtgc
    38161 tcactcctac accaatacga gtcatttatc aaatgatttc tgcttttagg acggtccggg
    38221 ttcgatccga aacgcgggcg cgttcgctca aacatacccc cgcaacccag agcacgccgt
    38281 cgtcatcgac gaggagcgga acgcttcctc gctcctcaag gggcaccttg gcatcgagca
    38341 gcagcctccg gaccttcttc gttccggcca tgcctagggg ctggatgcgg tcgcccgtcc
    38401 gcacgccgcg gcagacgagg ggaaaccgga tccgctcgta atcgaggcag gcgatctgac
    38461 ttccggtcgg aaccggccgt gtgacgcccg cgacctcgaa acggaggcgg catccgggtg
    38521 cgagggtcag tacgcccgga acagggacgt cgaactgaaa gccggcttcg gcgtccgtgc
    38581 tttttcctcg caggcgggaa ggcctatgtt gcgattttga ggccggcaac gagacgtcgt
    38641 cggttttctg aaaagagagt tgatcgtagg atcgcgtcac ggtaatcccc cccgccagat
    38701 ggacttccgc atatccgtaa cggccgagga ggttccggac cagttggata tgcgcatggg
    38761 tgatgccgga gggcgctccc acgagcctgc ggacgacatg aaggatcaag cgattctgga
    38821 tcgcttcgtg gagggtcagg aacgcgggaa tatcgaaccg gatcctcccc tgcccttccc
    38881 gtccccctgc cctggcgtag gcctccggaa ggaccgactc catgaagcca ttttcgagtc
    38941 ccgcgatctc cgccatccgt gtaaggccgc gcacgatagc gggattgtag accgcttcga
    39001 gctgtggcag cagatcgtgg cggatccggt tccggaggtg gtggacatcc cggttggacg
    39061 catcgttcct gaacgaaagg gaccggcatt tgagaaaggt gcggatatcc gcaggatcca
    39121 tgtcaagcag gggacggata tacctcccgt cccggatggg gaggataccc ttcagcccct
    39181 cgatcccgct tccccggagg aggttcatca gaaccgtttc cgcctggtcg cggcggtgat
    39241 gtcctagggc gattttgccg gcctggattt cacgggctgt tgcgtcgaga aaggcgtagc
    39301 gatgttcgcg acaccagtct tcgagggagc ggccgcgggg gcggagtgac gcggtcgtca
    39361 tacgaccggt ccgcagctca accccccact tcacggccag ttccgagacg aatcgttctt
    39421 ctgcgtcgga tgcccgcccg cgcaaaccat gattgaagtg agcaaccgtc aaagagagat
    39481 cgtaccgcga cctgaggtcc atcaggcccc gcagcaacgc gacggagtcc ggacctccgg
    39541 aaacggccac gagcacgcag tccccgcgtt ccagaagacc gcgcctgcgg accgtttttt
    39601 caagagccct gacgaacatc tgttcctcca agcatgccct gtaagttggg ctgttccacg
    39661 gttcatggct tacgatcatg cgaagagatc gaccaattcc gacaggcgct cgcaggcgtg
    39721 atccggttcg agcgcaagca agtcatcccg gtcgctcagg ccgttcagaa gtgcgcagct
    39781 gatgaagccc gcgttcttgg ccaggaggat gtcgttgacg ccgtcgccga cgaccagcgt
    39841 ccgtttcgga tccggggcaa cgatctcata aagcggtgcg agcacacccg tgtccggctt
    39901 ccggtgggtc gtatccgcgc caatgatccc cgtgaaataa ccccgtatcc cgagacggtc
    39961 gacaatcgtc cgggctgccg cgataggctt gttagtgacg atgaccttgt tctgacgtgc
    40021 gaaatacgtc aggaccggat gcacgtccgg gtagaggtcc gtcgtatcga gcatgtggcc
    40081 ggcgtaatgg gcggaaaaca gatccagggc cttggcgacg agttccggcg cagcgtgccc
    40141 cagggatcgc tcgatgagca cccggacgcc gtcaccgatg aagcggagga tcttttccct
    40201 ctcgatcgtg ggttgggaca gcgcctggag ggtgtaatta accgaggcgg cgatgtcgtc
    40261 gccggagcgg atcagtgtgc cgtcaagatc gaagatcatg agggagaccg catgtttttt
    40321 tgcattcatc cgcaagtctc cttttcagtg ttgatgcgtg cgccggaggg cgtggattct
    40381 tccggaccct gcggcctgaa cgcccctgcc tttaggtcga ctatatcgga ccgggtgggg
    40441 aatttcaagc ccgcggcgca tcggattggg cgaatgacaa tgatgtaaag gcgacaagcc
    40501 gggcttgaag cgtctcagaa cttcatttct gttgaccgcc gaacgccgtc gtgatacgag
    40561 atggacaccg cttggatccc tttcggactg agacggaacg gtctgacatt tgggtgcagg
    40621 tttattctgg ggccttccgc aagctgggga ggtctttttt attctggcag ctgggataac
    40681 gtccatggaa aaggatacgt tcgccatcat cggcttgggc aaggtaggca cggccgtggg
    40741 cctgctgctt caggcgtccg gatacccgat tgtcgccgtt tccgatgtcg atgcggcggc
    40801 gttaaagcga gcatctcggt tccccggcgc ccatgccgtt gaggcttgct ctgaagccgc
    40861 cgccctcgcc cattgcatcc tgatcaccac gagggacgat cggatcggac ccgtctgcga
    40921 agagatctgc cgaaacggcg gcatccgccc cggtgacatt tgctgccata tgagtggcgc
    40981 gggcggactc gatctccttg cccccgccag acgtttcggc gcccaggtcg cctcgatcca
    41041 tccgatccag tcattcgcgg atgtagacgg tgcggtcgcc aacctcccgg gaagcacgtt
    41101 cggcgttacc gccgatccct cgattttgga ctgggcctgc caactggttc gggatctggg
    41161 tggggtgccg ttggtcgtgg cggagtccga gaaaaccctt taccacgcgg cggcgtgcgt
    41221 ggcatccaat tatctgacga ccctgctgtc catggtggag gacctctacc gttcgctggg
    41281 tgtggatgcc cagagcgccc gtcaggcctt ctggcccctg gtcctcggca ccctccggaa
    41341 tatcgaagtg cgcggtccgg tcgaggcgtt gacggggccg atcgcccggg gggatgtggg
    41401 aaccctccgc cgtcatctgg ccgccctccg gaccgggaag ccggaattcc tgagaatgta
    41461 ctgtctgatg gggctgatga cggtcgagct gagccagcgg caaaaccggc tgtcccccga
    41521 aatcctcatc gaaatgaagg accttctgaa aggaggatgc gaagatgagc actcacggta
    41581 aactcaacaa ggtcacgtcc gccacggtac gggacatgaa gcggcgtgga gagaagatct
    41641 ccatgctgac ggcgtacgat tatccgacgg cgctctgtat cgacgaagcc ggcatcgaca
    41701 tgatcctcgt gggggactcg ctggggatgg tcgtgctggg ttacgacagc acgcttccgg
    41761 ttacgatgga ggtcatgctt catcacaccc gggcggtttc cagcgcggtg cagcgggcca
    41821 tggttatcgc ggatctaccc ttcatgtctt atcagtgttc tcctgacgat gcggtgcgga
    41881 atgcgggtcg aatgctccag gagtcgggtg cgcagggcgt gaagctggag ggaggccgtg
    41941 atttcgcaga ggcgacccgg cggatggtgg cggcggggat tcccgtcatg gcccatttgg
    42001 ggctaacccc ccagtccgtt catcaactgg gcggttaccg ggtacagggc aaggatgacg
    42061 taggcgcgcg ccgcattctc gaggatgccc gcattctcga agaggccggc gccttttccc
    42121 tggtgctcga gtgtgttccg gcttccctgg cggcagacat caccgccgcc ctcgccattc
    42181 cgaccatcgg gatcggtgcg ggaccgcggt gcgacggcca ggtcctcgtc cttcacgacg
    42241 tgatgggcat gttcgaacgc ttcacgccaa aattcgtaaa aaaatacgcc caggtcaatg
    42301 ccttgatgaa ggaggccgcg caggcctaca tcaccgatgt cagggagggg ggcttcccgg
    42361 gtccggaaca ttccttctga gtcgggattc gtcgtcaggg ctggtaaaga acgacgttgg
    42421 ccttttccct gagttcacgg acgagatggt tgaaggccgc ctgctgtttc tgacgctgta
    42481 ggtacgagga gatcatctcc ttcgcttggg cgtcgagcgg catggttttc ggcggatgct
    42541 tttccaggac ctgaatgacg tggtatccgt attccgtttc cacgaccggt ccgatggcgt
    42601 tgatcttctg agagaacgcc gcgtcttcga agggtttaac catttgccct cgggtaaagg
    42661 tccccaagtc gccgccggac tccttgctcg ggcagtcgga gttcgtcgtg gcgagctcgg
    42721 caaaattccc accggccgcg agctgcttct gcaaatcttc ggccttgttt ttcttggttt
    42781 gggccgccgc ggcgtcttcc cctgcggcct tggcaataag gatgtggcgc acccggacgg
    42841 attccggcga cctgaatttg tccgagttct tccggtagaa gccctggatc tctttttcgg
    42901 tcgctttctc tttcgccttc ggctgagaca gaaccagttt gttgatcttc aggccgaagt
    42961 cgatctcctc gcgtaatttc acctttgtga tcttgttctt tttcagctga gactccagcg
    43021 tcgtccccgg cgggaaactg gcctgcaact gctccatggc ctcgttcacc tcgcggtcgg
    43081 cgatctggat attcctgcgc ctgacctcat tcgagagcag ggtgcggacg acaaaatcct
    43141 cgatcacccg ctggcggaga cctttgcgcg cctcctctat cttcgcggcg ggaatctttc
    43201 ccttcatccc agatattttt tccttgagtt cccggtcgag ctgcttctcg gtgaggatcg
    43261 acccgtccac ctccacgacc gcgcccgcgg ggcccaggcg gggtgtcgga atttcagccg
    43321 attggggcgc tgacgacggg ggggcttgaa ctccggctcc tccggacaaa ggcgctgcct
    43381 tctcttcctt cgacccgcag gccgaaagga gcagcgtgag ccccagcagg acggcgacga
    43441 ctccccacaa cggtttttgc ctcatacagc ctccctcact cttgatcatg gtcaggcctt
    43501 gaacggttgc cgaatcgggg acggccgaca ggcgctgcct cacatcgtca ttcgtcgcat
    43561 cccggacgat ggggcgcgtg aattcatgac ttgatcggga gtatatgaag aaaccacgaa
    43621 cgtcgtcaag aagatattga gttcagaacg cgccccggag tacgttgggc gcttccacgc
    43681 tcctcaaggt cataaggcag gcctcctgtg cttgcagatt gccgctgaaa ccggcctctc
    43741 cgggaccggg gccgttttgt ccccatacgt tcatcagcca ggacagggcg gccgccatgg
    43801 gctgcggggg gtaagcggat gctgcgagtt cgtgcacgag cctcagggtg cggaacccgt
    43861 cgaaccaggt gtggaaggcc cgctgggcgg ccgcgtattc ccggacttga cgccggatcc
    43921 gcggccagac ctcggcgaag ttctgccgct cgaggaatcc ccgcaatccg gggtgcaggc
    43981 ttgaggcggc ctcgaggatc tccttcgcct ggagttgcca accctcatcc atcagcgcga
    44041 gccatttacg aaggatggcg aagatttccg ggtggtagaa gagatactcg tcccgctccc
    44101 cggccaaatg gcggatcatc cgcttccccg ttccgaatgg aacgcgggat gaaatacggt
    44161 cggacgggcg gacgcaggta tcagtcaatg acgaaatcgg accggccttt cttagcttat
    44221 tcagaaaata gaaatcctct ccggcggtac gtcggctcat gcctcgtacg gcgacgtagc
    44281 ggtcaggcgt gaagaccatt gtcgagccga tgctgtggaa cgcatagggg gatgctgccc
    44341 agaacagacc caagacgtag tagcgcaaaa aaagttcgta gcagacaatg gccgcgcgac
    44401 ggtctgtgtc gtccggaagc ggatgattga aggcgatcac agcggcccca tgctccggat
    44461 cgtgaaagtg ccgtcggatg gccgggagat aatcgggcgc cacgatcgta tcagcgtcta
    44521 ggcagaccaa aacgggacgc tcccggggcg agggccgcag ggtgagcgcg agatcgagac
    44581 cgattttccg tgcggtgccg acccccgcca aagacgacgg gatttcgcat cccgtcgacg
    44641 aggcgtcgat caggccgata cgcaacccgg atcctcgcag gaagtcgatg acgccgcgca
    44701 gccctccgtc ggtatattcg ctcacccggg tgcgcccggc gatcaggccg ttcaagatgg
    44761 cgatcgtctc ctgattgtcc gtaaacattt cccgatcgac ggtttcgcgc ggcccgttgt
    44821 tgacgacgac gatcaccagg gtctcagcga gctgatccgc gggattggcc gcaagcgccg
    44881 ccagcgtgtg gaagaggtgc cctttctccg ccagggcggg gatgacgacc gtcaccgtga
    44941 cctcggatgg atcgccgcag acaaggcgcc aggggccgtg cagaacgtat cgatcgagat
    45001 actttttcag cctttgttgc ccacggtcat gccttccagg tgtgctagga caagcttccg
    45061 tagagccggg tagtcgtccc ccggaatcca gtgtatccgt atgccgcgtc tttccatcct
    45121 tcgaaaccat gtgcgctgcc tcttggcaaa ttggtgaatg cgggtattca actcctcata
    45181 taggcggtct gcgttgatcc gcccttgaag aaatctcgcc acatagcggt attcaagccc
    45241 gaagcgatcg atgctttccc acgacaggcc gtcatcgtga agtcgccgga cctccgccac
    45301 gaggcccgct tcgagccgct ggctcagacg cctggcgatg cgctcccgca gggcatcgac
    45361 ctccatcgcg atcccgatca ccagggggtt tagatccgtc ggcctgtacc cggaatccaa
    45421 aggcccggat ggggcatgct cggcaatctc gatggcccgg atgaggcggt cgcgatcgac
    45481 caggtcggtc gtgttgtgga ggtcgggacg gctgcgccgc agccgctcgc tcagttgtcc
    45541 gttgtccagg cccgatagaa cggcccggag agcctcgttt ggcggtactt cttcgaaggg
    45601 atagttcatg aggacggctt cgagatagag gccggacccc ccgacgagga tcggaaacgc
    45661 accacggttg cggatttcgt cgaagcgttg aaagaagagc cgctggtagg caaagacatg
    45721 aaactcgtca tccggcgcga cgatatcgat caggtggcag ggtaccacgc ccgcctcgga
    45781 gcggtattcg tccaggtcct ttccggtccc caggtcgagg cctcggtaga cctggcggga
    45841 atcggcggaa aggatttcgc ccccgatctc gcgcgccagt cgtacgccaa gggctgtttt
    45901 tccacaggcc gtaggcccga ggatggccac gaggtttacc gtcattccca cctccccggc
    45961 gcgataaaaa aaccccggtc aggcgtgccc tcccggggtc ttcgatgatt cgatgatgcc
    46021 tataccgtct gcacgttggc ggcagccggt cccttcttcc cttcctcgat gtcaaatcgg
    46081 attcgctgcc cctcgtacag ggttcggaaa ccggaaccct gaatggcgct gtagtggacg
    46141 aacacgtctc ccccctcgtc gctctcgatg aaaccgtagc cctttttctc gttgaaccac
    46201 ttcactttcc cctctgccaa agccttaccc tcctttcttt ggtcaagaaa tcaaaaagcc
    46261 atccggcctc ggactcaaaa gatccgcgcc ccgatggctc ccccttcagg cagtttttaa
    46321 ataatcgaac gctcgcatgc tccatccgcc gggataacct gaataataga cccaacacga
    46381 cactcccgac gattcgcccg tatcaccata attttcaaaa atcaagcatt tttgaggaat
    46441 gaatttaaaa tagttttgat cgcaatgacg agcgcctctt cccggccgat gtagaaatga
    46501 tccgttcccg tcagaacctg aggcacaagg ttgatccgtc cgagacgctc cgtcagttct
    46561 tgaacgtcac agaatgtatc gtggtcaccg cagatgacgc cgatcagtcc ttcggcgagc
    46621 ggatctaagg gggtttccaa gaaatcgatg gggggagaaa ccagtaggag gcgacacgcg
    46681 agaggctcgc gggcataaag gctggccgtc acccaggccc cgaaggagta gccgcagagc
    46741 agaaggggcg ccgacgtgcg gccggccaga aaatccattg cggcgcggag gtcgtcctgt
    46801 tcgccacgtc ccccgtcata ccgtccctca ctccgtcccg tgccgcgaaa attgaaccga
    46861 agggtcgtaa aaccgagaca ccgaaagcct tcgcagacag cctcgacgac attgttgtcc
    46921 atactgccgc ccatctgggg gtgcggatgg gaaacgacca cgcctccccg gccccttccg
    46981 tgagccatcc tcccctcgat ccgaatgctg ccgacatcga ttgagaccgc ttcttccatc
    47041 atagacttcc cttcttccga atacaggcca cccggtgatc cgcttccggt tcgaagagtg
    47101 ttggatgcca ttcccggcac cggtccaagc gctccgggca acggggataa aacaggcacc
    47161 cttcttcaga ggggggatga tcgtcgattt ccgccggctg cccggggcgc tttgcgcggg
    47221 gatcgggaac gggtatcgcg cggtgaagga gacgtgtgta gggatggagg ggattcgcga
    47281 aaatcgcctt gttcgatgca agctcgacga tccgtcctaa gtacatgacg gcgattcggt
    47341 cgctcatgta gcggacgacg ctgaaatcat gcgtgatgaa cagatacgtt aggccgaaat
    47401 cccgctggag ttgcttcatc aggttcagga tctgggcctg aatggaaacg tccagggcgg
    47461 agacgggctc gtcagcgacg atcagtttcg gcttcagggc gatcgcccgg gcgatcccga
    47521 tgcgttgccg ctgaccgccg ctgaattcgt ggggataacg ttgggcctgc tcttcgctca
    47581 gccctacggt gcgcatgatg ctccgggtgt tctccgggac aagcctcgga tcggtggcat
    47641 gcagcaaaag gggctccatg acggtctggc ccgccgttcg acgggggttc agcgacgcgt
    47701 agggatcctg gaaaaccagt tgaaccgctc ggcgaaaagc cttccgtttg ggcttcgtca
    47761 aggtgaagat attgcggccc tcgaatctga cttcaccgga ggtgggcttt tcgagcgcga
    47821 ggcagagccg ccccagggtg gatttcccgc agccgctttc tccaaccagc cccagggtct
    47881 ccccggcgcg gatcgacagg gtgacgtccc ggacggcgcg gacctcgcgg gcgcctcttc
    47941 ccagtccccc tccggagacg tgaaaggtct tcgacagtcc ccggatctcc atcagcacat
    48001 cgcgcatcgc gtccctcagg gatgcccgcc cgggcgggaa tcagtcttgc ggaaacaggt
    48061 tgatctcttg aacacggtca tcatcaggcg taattccagc atcgcacggt tccccccggg
    48121 gagggcgtca acagcgccgg ttcctcctgg cggcacaccg ggcgaacatc ggggcaacgg
    48181 tcctgaaagc ggcatccccg gggaagatcg tacaggctgg gaacctgtcc cggaatgatc
    48241 ggcagcggcg tcgcggggtc ggttccgagg cgcgggaggg aggcgagcag gccctgcgtg
    48301 taagggtgcc ggggcgtgtc gaacatcacc tccgccggag cctcttccac caccttgccg
    48361 gcgtacatca cgatgacgcg ctcggccatc tgtgcgacca cgccaaggtc atgggtgatg
    48421 agtatgagcg aggttcccat ctcccgcttc aattgatcga gcagggcgag gatctgggac
    48481 tgaatcgtga cgtccagggc cgtggtcggc tcgtcggcga ggatcaggtc ggggcggcag
    48541 gctaccgcca tggcgatcat gatgcgctgg cggagaccgc cgctgagctg atgcggatag
    48601 gcacgcaggc gttgatccgc ttcggagatt ccgacccgat ggaacaatcc ggccacctca
    48661 tccagggcct ggcgacgcgt ggtctttctg tggcgaagga tgagctccgc gacctggtct
    48721 cccacccgga agacgggatt gagcgaggtc atcggttcct gaaagatcat cgagatccgg
    48781 tcgccgcgca ggtcctgcag ccgcttccga tccagtgcga ggagattctc gccccggaat
    48841 aggaccgatc cggaagcgat acggccgggg ggcgtgggga cgagccgcat aacggacagg
    48901 gccaggacgg tcttgccgca gccggactct ccgacaaccg ccagggtctc cctccggccc
    48961 aggacgaggt cgaccccgtc cacggcccgc accgttccgg cgggggtatc gaagacagtc
    49021 cggagatcgt taatctcaag gaggggtggt gcggtcatga agcgtctccc gttcctttgc
    49081 gtgcttaggc gaaagggcca caaaagtcaa cgaggaaaag tgcttgaatt ttcgagcaag
    49141 atgtatatac ttcagcctca aaaacgattg taggcggatt ccggtcggtt gtgacgaagg
    49201 ttttatttgt atgtaacgcc aatatcgtac gcagcttcat ggccgagcgg atattcaatc
    49261 ataggctcgg acggtcgagc ctttccgggg tatcggcgtc ttccgccggc ctgctgaaca
    49321 tgaacggggc accggcggac acaacggcga gacgaatttt ggccgaacac ggcattgatg
    49381 acgaaggcca tgcctcacga ttcctgaccg gggagatcct ccgtgaagcc gacctggtcc
    49441 tgacaatgga gctgccgcaa ttgcggaaaa tcgaatcgca ataccccgaa ttcgccggcg
    49501 ggctgagagt cctgaaatcc tacctgccgg attacgatat ccaactgggc gacatcgccg
    49561 atccctatcg tcgctccgtg ttccactatc gcctgtgctt cgccgagatt tccctttccg
    49621 tggaggctat gatcacatgt atttagaata ctggggtttg cagaagtttc ccttcgagaa
    49681 cgttcccgat cccgagttca tgtactactc cacggagcac gaagaggccc tggcgcgatt
    49741 gctctatgtc gtcaagcgaa acaagggcgc catgctgctg accggcgaga tcggatgcgg
    49801 caaaaccctc ctgagccgcg tcttcatccg cgaactcgcg gaatcggatt acgacattgg
    49861 gctcatcacc aatccctcgc tgatacccat ggatttcgtc agggagatgc tcttccagtt
    49921 cggcgtcaac acccaggcca cggccaaaag cgaactgctc accgtcctga acgaacgcat
    49981 gatgcagaac gtccagaaca accggacgat gctgatcatt atcgacgagg cccagctcat
    50041 tcccatggaa accctcgaag agattcgact gctccttaat ttccagctca acgaccgcta
    50101 tctcctcaat ctgatcctta tcgggcagcc cgaactccgg gaaaagatcc gcggacacaa
    50161 gcagctcgat cagcgcatct tcatccgcta ccacctcaat cccctgaatc tggaggatgt
    50221 ccgaaactac atcgccttcc gtcttcagaa ggccgggcgg ccggaaaaca tgttcaccca
    50281 ggaggcgatg gacgagatct tcagccactc cgacggcctg ccgcggaaga tcaacaacat
    50341 ctgcgacctg tcgctcctga tcggcttcag catgaagtac accaaactgg acgtcgagat
    50401 catccggaag gtgattcagg attccatgta gcttggggtc ggacgctgac gtccttcagg
    50461 ccgctcaccc cctgcgtccg cttgtcttcc cgacttttca acccgtttca gtccacggac
    50521 cttcccctca gcaggtcggg caggacgcgc acgagatgtg cgacgatctc gataaccctt
    50581 gcccgctcga gccacgaggc gcggtgcgcg tcgtgtgccc cgcgacgctg acgctggatg
    50641 atcctcggcg ggaagagggt tctcagggaa aaatggatct ttttcgcggg gccttcgttc
    50701 atggacaggt atagcaggta actgctgccc cggatccgga ggccgcgccg ctgcagcatg
    50761 ctgaaaatcc gctgctcgaa cgtgatccgg gggggacgca gccgatcgag aatccccgcc
    50821 ggcaacgtca caccggcatc gtgtaccacc tgcttaaggg cgaagtagac cagtccggag
    50881 aggtgaaacg cccccgcctc ccgggcgacg gcatcccagt cgaggtgctc cgcgaaggta
    50941 tcgagcagac aaacgaggtc atacaccagg atcagccggt cgaaggtgtg attcaccctc
    51001 agggcgtgtt cgcacagata gatcaacaga tgctccggtg aaagtatttt cgtctccacg
    51061 tcatcgaatc ggacggtgac ggcccgttcc caaagggaag cgacgtcgaa aaagggtgca
    51121 aaagcctcgg ccggcaccga ggtgttcacg gggtgccagt ggacgtggag catcggcggc
    51181 atccgatcgg cgcggtggta ttccaggctg gcgagatacc cctcgggatt cgcgagggcc
    51241 acggccgcat cgccgtcgac ggcgcggtat cccaatgcct ccaggcaggc gttcacctgg
    51301 aggagttcgt ccttccggac caggaggtcc acatcggaca tgccccgcat gcccgggctg
    51361 gggtagagat attccgcgag ggccaggcct ttgaggagca ggtggtcgag ctgggccgcg
    51421 ttgaatccgg ccaggaccgg cttgagacgg tgcttcaggg tcaggttggc gatcaggttc
    51481 ctttggaagc gtccggcaag gttctcccgc cagaaggcgg gcaaggcaag gtcgagacct
    51541 tttttcttga gacgataata gacggatccc tccacgcgct cgcgttgaca tcgcgcgacg
    51601 aaaccttgcc agaatgcgtc atcggcctcc ggcaggcgag gcgacgcccc aaaaagaatc
    51661 tcctggatca ggcgcagctg ggtgtcgttc gatgacgtcg ttttcatggg gcgcccctga
    51721 caagggcggc gtaggccacg ttcgaggcca tcagctcggc atgggcgccg atggccgaca
    51781 ggcgacggtc c
//
LOCUS       DAOOLJ010000012        49321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_498_length_49321_cov_19.059981, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000012 DAOOLJ010000000
VERSION     DAOOLJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 49321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49321
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_49321_cov_19.059981"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLW16_03905"
     CDS             <1..519
                     /locus_tag="PLW16_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPB70082.1"
                     /translation="ERPGRGRTGQIADPHIWLSPRLMKTQAQTVAAALGRLDPAGANA
                     YDRNLRTLADDLDRLDRRLAARLGPWRGSRFYVFHPAFGYFGDAYGLIQVPVEIEGKE
                     PSARQLARLIRSARGDGVRMIFVQPQFARKKADAVARQIGGVCIPLDPLAADYPGNME
                     RIATALEKGLVH"
     gene            534..1334
                     /locus_tag="PLW16_03910"
     CDS             534..1334
                     /locus_tag="PLW16_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70083.1"
                     /translation="MQDTHDPGAAAVPEEPVIDLKGVSFAYDGPPVLEDVTFQVRARD
                     FLSIVGPNAGGKTTLIKLILGLLQPTRGSIRLFGEPPAKGRRRVGYMPQQAALSIHFP
                     VTVIEVVLMGRLTAGRNPVFYRRADRAAGAEALARMGLADLKDRPFSALSGGQRQRTL
                     IARALVSAPDLLLLDEPTANVDAARETEFYELLERLSADITIVLVTHDLGFVSRYVRS
                     VACVNRRVLVHPTSDITGEMINQLYGSDVRMIRHDHRCAPEGHAWSNS"
     gene            1319..2209
                     /locus_tag="PLW16_03915"
     CDS             1319..2209
                     /locus_tag="PLW16_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPB70084.1"
                     /translation="MVQFLMDLGQFGFLRNALAVGLLASVACGIVGTYVVTRRITAIA
                     GSIAHCVLGGLGVARWLQTVHHLEGLHPLYGAVIAALLAAVLIGWVSIRAKRHEDTVI
                     SAIWAIGMAVGILFIFKTPGYNEDLLSYLFGNILMVTDEDLWMVAGLDVVIVAVSLLF
                     YHPFLATCFDEEFARLRGVPVERYYILLLCLTALTVVLLITVVGIILVIALLTLPAAI
                     AGTFSRKLWHMMILAALTTAALTAGGLALSYGPDLPAGATTIALAGVAYLAAVLGKRL
                     FGRRRAIGASGGCPPGEELS"
     gene            complement(2265..2816)
                     /locus_tag="PLW16_03920"
     CDS             complement(2265..2816)
                     /locus_tag="PLW16_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPB70085.1"
                     /translation="MKPIGPLMWEHRLIEQALSAAGLEIERIRERREVNPVFVDVIVD
                     FIRTYADRTHHGKEEEILFRDLAKKPLAAEHKRIMDELVAEHVMSRQTVGRLVEAKER
                     FLQGADTRAEIAAGLTTLAEFYPKHIEKEDKHFFFPCMDYFTTAELAAMLDEFWAFDR
                     TMIHEKYGRIVETLTGQKAILPK"
     gene            complement(2869..3462)
                     /locus_tag="PLW16_03925"
     CDS             complement(2869..3462)
                     /locus_tag="PLW16_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB70086.1"
                     /translation="MTGNKAAPKVLMLLGSPRKKGNSAILAEQIARGARSAGATVESV
                     YLHGLNIAPCRACYACQKPRSRGCAVDDDMQALYPKLIEAEAWVIASPVYWFTLSAQT
                     KLFMDRCFALLAYASNPFAGKRIAIAMTYGDTDPVTSGCINALRTFQDAFAYTKSPIV
                     GMVYGSAPGLGDIRKNEALLREAEALGRTLVSPDPGR"
     gene            complement(3523..4179)
                     /locus_tag="PLW16_03930"
     CDS             complement(3523..4179)
                     /locus_tag="PLW16_03930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB70087.1"
                     /translation="MKVLQCWDDGLVDDIRLADLLRRHGARAAFALNPGLYGPERSLG
                     WRAGEREVWRLALPELARVYDGFEIASHALTHPYLTDLPPDRLWEEVAESRRRLEDLF
                     RKPVRGFCYPFNAVNDAVRQAVRAAGYAWIREDGEAGGEPGLPAPRVFRPACHALAAD
                     FWTRYDAARARGGAFFFWGHSCDLGNEAMWRDFERKIAAISADPEAEWCDPGDACGSV
                     "
     gene            4397..4576
                     /locus_tag="PLW16_03935"
     CDS             4397..4576
                     /locus_tag="PLW16_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB70088.1"
                     /translation="MDAKEKAVLDVLKKEGKPMRPGDIAEKAGLDKAAVAKIIDKLKK
                     EGKVASPKRCFYAPV"
     gene            4608..5369
                     /locus_tag="PLW16_03940"
     CDS             4608..5369
                     /locus_tag="PLW16_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPB70089.1"
                     /translation="MFFYLSKILDFVLLPVLWIGVVLLVAALTKQRRRKQRWLVAGLA
                     LLIVLTNPFLVRVALNQWERPYRTAAIHQPYDYAIVLGGFSYWDGRYGRLTFGEASDR
                     LMQALDLYRSGKVRKLVLSGGPGSLFNTEEREAAYLKAYLLRSGIPERDLLMESNSRN
                     THENAVFVARLLEGRGGSSLLVTSALHMRRAEACFAKAGLAVSTWPTDRLTPPEARAF
                     EPGYLFLPSSTALDHWERLVREIVGYGAYALAGYV"
     gene            complement(5495..5770)
                     /locus_tag="PLW16_03945"
     CDS             complement(5495..5770)
                     /locus_tag="PLW16_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70090.1"
                     /translation="MKKTLGSLVVAAAVVLMTATFGFAEFAAGGADEFPYFQLGGLII
                     GGLIITSLKMKYEKMYAGEAVGAFAMYAILIALFTNPVITLIKDMVG"
     gene            6012..>6725
                     /locus_tag="PLW16_03950"
     CDS             6012..>6725
                     /locus_tag="PLW16_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB70091.1"
                     /translation="MNAPVDTLPLYPRLDEIAEAIARSGPLVLHAEPGAGKTTLVPGR
                     LLAHEAFAGRKILLLQPRRIAARAAAERIAALRGERLGETVGLRTRLETIAGRTTRLE
                     VVTEGVLTRILQNDPSLAPYQLVIFDEFHERTLQGDLALAFCWEARRVFRRDLKVLFM
                     SATPPTEDLQAALGALEVRSVAGRSWPVEVLDRPPLAGETPWAGAARLALEARDRLDR
                     TGGGDVLVFLPGFREIRLAR"
     assembly_gap    6726..6825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6826..8355
                     /locus_tag="PLW16_03955"
     CDS             <6826..8355
                     /locus_tag="PLW16_03955"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HPB70092.1"
                     /translation="ARQRQGRAGRLGPGICLRWWRSDAPRRPFSPPEILEADLAPLLL
                     ETALWGAVSPLNLTWITPPPAGALQQAGTLLKALGLIDGAGRITASGRRAAGLGLHPR
                     LACMVLNALARGWLPTAAVTAALLEEGDPLGGDDPDFRDRLAAWAARARGVKSALRPD
                     VAQRIGREAERILRAAGPGAGVLRAAEIDPDLAGRLLLFAYPDRAAQRVGGGHLGEAA
                     RLVLATGRGARVTGPLAREAFLVAADLDGGETEARVFLAAPVTRGEIEGGLAGQPETV
                     HLFAWEGWVPRSRTEVRVGRIVLVEARGAAAPPEALRQAARERIGRDGLDGLPWSEAS
                     RRLLARCRFVEARGRREGWPGFSDTVLLAEVEGWLLPFGRWDGGAVFSPEALLHALDG
                     RLGGKNRRALEALAPEFLALPSGTRRRIDYESGAVPVVAARLQEFFGCRETPRLCGEP
                     VLLHLLSPAGRPVQITRDLDGFWERAYPEVKRELMGRYPRHPWPDDPRSAPPTARTKR
                     K"
     gene            complement(8431..>9870)
                     /locus_tag="PLW16_03960"
     CDS             complement(8431..>9870)
                     /locus_tag="PLW16_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB70093.1"
                     /translation="IDREILDRALARLAADRRIILEAPDPGPADARETDPAVYLAGYF
                     LAETLLARHLRTLVETPRAVREIDAEKAIPWLEAKLGIVLAEKQVEAIRAVAGHKVLV
                     VTGGPGTGKTTIINAMIRVFAGLKKKILLAAPTGRAAKRMTEATGHEARTIHRLLEYS
                     FQTGGFLRNADAPLDGDLVIIDEASMIDTLLMHYLLKAVPPRATFVLVGDVNQLPSVG
                     AGNVLKDIIDAGVVPVIELNEIFRQAGESSIVVNAHRINAGQMPALATSRERLDDFYF
                     IEQEDPGRVLELIITLVKERIPRRFGLDPIDDVQVLTPMHRGIVGGINLNAELQKALN
                     PGEEGISRMGRLFRAGDKVMQISNNYDKEIFNGDIGRISGIDREAQELTVSIDGREIL
                     YDFSELDELAHAYAVSIHKSQGSEYPAVVIPILTQHYVLLQRNLLYTGVTRGKKLVVL
                     IGTKKALAIAIGNNRTEARYTRLRARLRA"
     assembly_gap    9871..9970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9971..10775)
                     /locus_tag="PLW16_03965"
     CDS             complement(<9971..10775)
                     /locus_tag="PLW16_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB70094.1"
                     /translation="MANEPLAELRGQIERITYANEENGYTVARVKVPGRKDLVTVIGH
                     IVNPTPGEVLSMRGEWGRHPKYGEQFRIVSCESGVPATGSGLEKYLGSGLIRGIGPVM
                     AKRIVRRFKGETLHVIETAIGRLAEIEGIGPKRIAMIRKAWDEQKEIRAIMIFLQDHG
                     VGAGYAARIFRHYGNDAIAVVRENPYRLATDVFGIGFLTADRIAEKLGFARDSEFRAE
                     AGLFYVLHVLTEEGHVYYPREPLIERCREMLAIDREILDRALARLAADRR"
     gene            complement(10921..12600)
                     /locus_tag="PLW16_03970"
     CDS             complement(10921..12600)
                     /locus_tag="PLW16_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70095.1"
                     /translation="MKAERFWKKHWDEGLTDLDPARFETTYTELVRPIFRKFPEKPAC
                     AYFGRQVSYAELDRAANRFAHMLLTEGFQKGDVVGISLPNMPEYLFAWLGTLKAGCVV
                     SGVSPLLTTAQVAYQLRDSNAKGFVTLDITFADKVPELTAQLEALRLIVTARAGSFLP
                     GLEGKVTPIAGRTVSPFEEILEGGKYSTDEPGVEITPDDIAYLQYTGGTTGMPKGAML
                     SHRNCVADLLIYQTWLGWKEGEVTALCGFPFFHIAGIFVNANGIYLGGLQILIPNPRD
                     TEHICAELEKYKPSLLVNVPALYQLLMANPKFRQMDHSHVETCISSASPFPEEAQREF
                     EKIVGKGKLLENYGMSETSPLIVSNPVKGKKKLGTIGLPLLNTDIRLLDPETGREVAV
                     GEPGEMCVKGPQVMVGYYGKPEETRNVIDADGYLHTGDVVVQDEEGYLRVVDRTKDMI
                     IVSGFKVFSVKVEAILAEHPAIASVALIGIPNPERPGSELVKAFVQRDPGHAFEGDEA
                     ALTEDILAFARAKLAPYEVPKTVEFRKELPLTPVGKLDKKLLRREARGDAA"
     gene            complement(12698..14365)
                     /locus_tag="PLW16_03975"
     CDS             complement(12698..14365)
                     /locus_tag="PLW16_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70096.1"
                     /translation="MEERIWHKAYVAGVSPTLDIERITLYEILARTAARYPEQVALIM
                     MGTEITYRQLHANVDRFAGALAGLGIRKGDKVALILANMPQMVIATYALFRLGAVAVM
                     NNPLYTETELEHNLRQSEARMAVCLDFLVPRVLSLKDRTRLEAVVACHIREYLPDPGN
                     DPFVQELYALHMDVSPQEGVYEFRALLEGGGTPPETRVAFDDPAAFIFTGGTTGVSKA
                     VVISHANSSTVVQQFKEWIFDAEDGMERLLAVLPFFHVAGYTDIMNNAIYRGWTAVLV
                     PRPTPDVIIALTLKYRPGLFGGVPTMFVGLLNNPKFKSAKLDFIKGCLSGAAPVALET
                     IRAWEEATGSTIVELYGLTETSALATFNPWRGKTKLGSVGVPVPETDCRIVDLDTGTR
                     DLKLGETGEILIKGPQVTRGYYNDPEATASIRDGWLATGDIGYLDDEGYLFIVDRKKD
                     VIIAGGYNIYPREVDEILYEHPKVQAACVVGIPDAYRGETVKAFVVLKAGQTETEEEI
                     VAFCREKLAAYKVPKSVEFRTELPQTSVGKILRRALRDEEITRQKGA"
     gene            complement(14431..15135)
                     /locus_tag="PLW16_03980"
     CDS             complement(14431..15135)
                     /locus_tag="PLW16_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB70097.1"
                     /translation="MGKADSHREAIRERKRQERERRIRDILDAAKRLFFQKGYQKMTM
                     NDVALEVRVSKPTIYQYFKSKDDLFFSLLAPASEKTEQALERIERHLMSGGYPTGAAL
                     LHDTFQALYGVYWTDPEAFRILQLFQHSGMVGVLDEDVRREFVAKARLTFEQGRRILS
                     AAMDQGLLRRMDVHPFNDVVWALFVGVIQLEDSKGGGKGESPFLKPTLELAERLVIAA
                     TAADGRPVANTPSNDT"
     gene            15412..15843
                     /locus_tag="PLW16_03985"
     CDS             15412..15843
                     /locus_tag="PLW16_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB70098.1"
                     /translation="MIENLSREQLAAILDALPIEFIFIDTQEKLLYANKGERRSRPAP
                     TDVVGKDIRGCHQPASLPLVERFVDNLKSGRKDEEEFWLLFPDRKILNRFLAVRDKAG
                     RYLGMIEYLLDFKALEDLAEDRKGAHARDYSTPPPSGIPVT"
     gene            15988..17586
                     /locus_tag="PLW16_03990"
     CDS             15988..17586
                     /locus_tag="PLW16_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70099.1"
                     /translation="MMEKTALAKQGIRSNDTGRPAYDLVIIGAGISGLSTGLMWLKNT
                     QGKKTLIVEKNPYPGGYVTAYERNGYVFETTQLFPDIVNILDYLGLEVPLKPFPGTFM
                     RRLVVHGNGVTEYRIPAGVENFTVYLCEQFPGDAAKIRKLMAYSVEMFAQVRKLKALP
                     TLGDMLTTPFAAPKVVANLNRTYAGLLDRFRITDDRLREVLETFSSFSGVPSARASAI
                     LTAGAMLSSMARCYRPYGFFDTFPAAMARRFQELGGEIRLRAEVERIVVENGRVAGVR
                     VKGDEPVIRAERVVTTVDPMVAMRRLVGEEHLPPKYLDRLKGIVMSPSSLNVALGLDD
                     GIDLARLDLDYPYNVVSTGRGTTERLFDAFLEGGNAFSPECFHMAVICPSLTTGAKNT
                     VTLRGVPFGIGDWLSWRQKDPGRYQAEKERWAEFFIGIAEKYFIPDLSRHIVVKDIAT
                     PATYARYSGSPTGSIYDMAALVTQFGPKRLPMKTPVANLYQPKFAHGLYGGMMNGVQV
                     VDLMLDRAFNGGNSLFNPQGRPAA"
     gene            complement(17689..17985)
                     /locus_tag="PLW16_03995"
     CDS             complement(17689..17985)
                     /locus_tag="PLW16_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HPB70100.1"
                     /translation="MATDSGIGYRIVATVTDVRGECSAGHRTGDTFEISCHNPGGLCG
                     FFYHDLFPALSTFQFGGSLPWWEGNTIEVPCPDSANRVTLRLERSTRPSDPAAG"
     gene            complement(17999..18655)
                     /locus_tag="PLW16_04000"
     CDS             complement(17999..18655)
                     /locus_tag="PLW16_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPB70101.1"
                     /translation="MAIIIILGFAVVLLGGLLLAEKKESLKGILLTKPLLSGLFIATA
                     LAQPHHAGPYFYLILTGLAFCFAGDICLAFFFNRRVFTAGLAFFLTGHLAYIVAFFIA
                     AGINAGTWGSVAAVSIISSYVFVRLRPHLGSMRGPVIAYVAIISLMIVGAASLAVRPD
                     YCVQARALVMTGSIFFYVSDLFVARHRFVKKSISNRYAGLPMYYAAQFMLAFSVGMIP
                     "
     gene            complement(18861..19670)
                     /locus_tag="PLW16_04005"
     CDS             complement(18861..19670)
                     /locus_tag="PLW16_04005"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPB70102.1"
                     /translation="MTEQLVTVSIENHVADVRFNRPDKYNALSLAMVQAIADTIDRLA
                     GEAAVRAVVLSGEGKGFCAGLDFENFAAMQSGEGAQLPRLTDRYGNRITNIFQHIAYG
                     WKQLPMPVIAAIHGVALGGGCQIALGADIRYCTPDAKLSIMEMKWGLIPDMSASQTVR
                     DLVSLDVAKELIFTGKVIRGEEAAQLRLVTRVCDKPYDEAIDLAREIAAKNPHAVRAA
                     KKLLNEVWHGDAARGLLMESELEMTLITTPNQLEAITANFAKRAPQFQDPE"
     gene            complement(19738..21972)
                     /locus_tag="PLW16_04010"
     CDS             complement(19738..21972)
                     /locus_tag="PLW16_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB70103.1"
                     /translation="MGTIHRTGCVLCPQNCGLEVEVENNRIVKVRGDKGNVRSEGYVC
                     RKGLNIANHQHNADRLKYPLKRVGDRFERISWEQAIDEIGGKIREIVDRYGPRSFAYM
                     GGGGQGCHFEAAFGVRLLRGLGSRYHYSALAQEFSGMFWANGRMHGRQTLLAEIDAEE
                     TDFLLVFGWNGMQSHQIPQAPRHLLRIAKDPDKSLVVIDPRLSETAKVANLHLAIRPG
                     TDALLLRAMIAIILREGWENRDYIAHHTSGLDAIRGLFTDFDARAAVRTCNLDFDQVH
                     EVCRQFATRKSCLRYDLGLLMNRHSAVSSYLAVVLQAICGRIGVRGGNVFHGQMMPLG
                     SHSDERDPKTWRTVTTNFFPVLGCFPPNAMPEEILSDHPERLRAVIVSGAHPLRSYAD
                     TTAYETAFKRLDLLVTAELALTETAVLSHYVLPSRSGYESWDGTFFPMTFPGIYFQMR
                     RPIIEPDGEPLELGEIHLRLADRLGLIPPIPESLYKAAEEGHAAFGQVLMDYAAQEPA
                     ALAAMPFVLGKTLGRAKGSVHLAALWGMLQTAPKPLRKNAARAGFTPGMGMGEELYQK
                     ILDHPEGIWIGRLDPDRNLDEIRTEDGRINLVIPELRAELETLDAAHEEAALVPDPAF
                     PFVLVAGRHFNMNANTLMRNPAWNAGKRDCTVLLHPADAEGLNVKDGQTVRVTTEAGS
                     VEIEAEVTETARKGHVVIPHGFGMVYDGKVHGANVNRLTKNTNRDWLGTPLHRYVPCR
                     IEAL"
     gene            complement(22009..22434)
                     /locus_tag="PLW16_04015"
     CDS             complement(22009..22434)
                     /locus_tag="PLW16_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB70104.1"
                     /translation="MKPEDCIFFQLAKAHQLGSRFLAQKVAALNITPVQALVLGFLAD
                     EDGITSSELGKRTELDSATMTGLLDRLEAARLLERRVHPADRRSIQIHLTDPGRTLAA
                     EAVRSIVEANREFLDILTERERKELRGLIRKLRDQSGRA"
     gene            22674..23900
                     /locus_tag="PLW16_04020"
     CDS             22674..23900
                     /locus_tag="PLW16_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPB70105.1"
                     /translation="MMNPIDTGDTAWILVCCSLVLLMTPSLALFYGGMVRRKNVLSTL
                     TLSYIFMAVIGVQWVLYGYSLAFGADIGGLIGGLNYLGLTGVDALPNENYARTLPHGL
                     FAAFQMMFAVITPALITGAFVERVKFKAFLLFSLLWATLVYDPLCHWVWGQGGWLKQM
                     GVLDFAGGTVVHIAAGFSALAFALVIGPRKGFGRSPMEPGNIPLTVIGAGLLWVGWFG
                     FNAGSALAANGVAVNALLTTNTSAATAGLVWMLLSWLDGRPSTLGIATGMVVGLAAVT
                     PASGYVTPMAAMLIGAVAAPLSYSTMRFRERRRLDESLDVWACHGISSTWGMLATGLF
                     ATIAVNGDGANGLFFGNPAQVGTQLVAIGVTIIFSFAATYVLARLLHASIGLRVSAAE
                     EEVGLDISSHGERSYS"
     gene            23939..24316
                     /locus_tag="PLW16_04025"
     CDS             23939..24316
                     /locus_tag="PLW16_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPB70106.1"
                     /translation="MLKKIEAIIREDKVTDVTDALAEIGIVGLNMFEVRGRGRQGGIQ
                     LVGRAGTYPVTLLPKIQINIVLSDRNLEDAIDTIRKAAFTGEPGDGILFVTPVEDVVR
                     VRTGERGQGAMMYPGDIDSKKAK"
     gene            24426..24499
                     /locus_tag="PLW16_04030"
     tRNA            24426..24499
                     /locus_tag="PLW16_04030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24458..24460,aa:Gln,seq:ctg)
     gene            complement(24629..26077)
                     /gene="purF"
                     /locus_tag="PLW16_04035"
     CDS             complement(24629..26077)
                     /gene="purF"
                     /locus_tag="PLW16_04035"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPB70107.1"
                     /translation="MKKAKGFRGCGTADGRPREECGVFAVYGHPDAARVAFFGLFALQ
                     HRGQESAGIATSDGCQIWERKGMGLVSEVFREDHLAKLPGRLAIGHVRYSTTGSSVIS
                     NAQPFLVHHGNEYYALAHNGNLINAQELRSQLEARGSIFQSTMDSEVIIHLMAPFFRT
                     RFEDALTRALYQIEGAYSVVMLTKDRVVAARDPRGFRPLCLGKLDNHGWVIASETCAF
                     DLVGAHYVREVAPGEIIVIDGEGLHSLTPFGPEKPAHCIFELIYFARPDSHVFGTNVY
                     LTRKRLGHRLAREYRPDADLVMPFPDSGNYAALGYAEESALPFEMGMIRNHYVGRTFI
                     QPTQPMRDFGVRVKLNPVRELLAGKRVLIVEDSIIRGTTSRNRVRNLRENGAKEIHMA
                     VSCPPTRFPCPYGIDFSSKGELIAAQKGEEAAIADFIGLDSLHYLTLDGMVAATGLAP
                     NSFCLACYTGAYPLTPPAELNKQCFETADLGL"
     gene            complement(26074..29376)
                     /gene="carB"
                     /locus_tag="PLW16_04040"
     CDS             complement(26074..29376)
                     /gene="carB"
                     /locus_tag="PLW16_04040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPB70108.1"
                     /translation="MPKRTDIHKILIIGSGPIIISQACEFDYSGTQACKALKEEGYEV
                     VLVNSNPATIMTDPETADRTYVEPITPEAVAKIIAKERPDALLPTLGGQTGLNTAVAL
                     AESGVLDAHGVEMIGATVAVIRKAEDREEFRHAMGRIGLRVPRSGFARTLDEAQAVAA
                     EIGFPVIVRPSFTLGGTGSGIAYNREELAEIAKGGLDASMIHEVMLEESALGWKEYEL
                     EVMRDRADNCVIVCAIENMDAMGVHTGESITVAPAQTLTDVEYQRMRDAAFAIMREIG
                     VDTGGSNCQFAVHPDTGEMIVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETMAAFEPTIDYCVVKIPRWTFEKFPETDDVLTTSMKSVGETMAIGRTFKE
                     ALQKAVRSLEIGRFGLVPPLPEGAGARPADFFAPKLVTPNSQRLFWIAAALGAGMDVE
                     EIRRLTGIDPWFLRQIGAIVEAEAELKAASPSVSLLRAAKTWGFSDRRLGELWGREEA
                     GIRDWRLAEGVGPVFKLVDTCAAEFEAYTPYYYSTYETENEARPSDRPKVAILGGGPN
                     RIGQGIEFDYCCVHASFALREEGVESIMVNSNPETVSTDYDTSDKLFFEPVTLEDVLH
                     ILATEGRGDVGAVPLIVQFGGQTPLNLARGLEAAGARILGTSPDAIDRAENRERFQEI
                     VRRLELNQPENDTAMDVAKALAAARRIGYPVLVRPSYVLGGRSMEIVYDDETLAAFMA
                     RALDVSPGHPILIDKFLDDAIEVDVDALSDGTTTVVAGIMEHIEAAGIHSGDSACVLP
                     PMTLSKALVKTIETQTKRIAAELGVIGLMNIQYAVKDGVLYVLEVNPRASRTVPFVSK
                     AIGVPLAKLATKVMLGKSLVDLKFTRRIHPAHIAVKEAVFPFNRFPNVDILLGPEMKS
                     TGEVMGIDRSFGIAFAKSQMAAGFPFPVAGRVFISVHDLYKDRIVPVARQFQALGFRI
                     SATRGTAAHLNRQGVRVETVCKLSEGRPHVVDLIKNGDIQIVINVSVGRLSTRDAVQI
                     RRSTLLYNILYTTTIAGARALGEAVRAMRAGPWDVAPLQAYHKTGGGRGTRGKRDAAV
                     VRQGTEGKRVKR"
     gene            complement(29380..30909)
                     /gene="guaA"
                     /locus_tag="PLW16_04045"
     CDS             complement(29380..30909)
                     /gene="guaA"
                     /locus_tag="PLW16_04045"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPB70109.1"
                     /translation="MILIIDFGSQYNQLIARRVREHRVYCQIEPPTIPLAAIRNLKPE
                     GIILSGGPSSIYEPNAPQADPGLFALGIPVLGICYGMQSMVHALGGEVVAARKREYGF
                     AELRVERAEGILAGITGTTRSWMSHGDAIGRLPKGFRVTARTANTPVAALEDPRRRLY
                     GLQFHPEVVHTTEGKRMIENFLFPVCGCLKTWTMQSFIRDAVEEIRAEVGDGRVILGL
                     SGGVDSSVAAVLLQRAIGRQLTCVFVDNGVLRLDEGQKVRALFAEHLAMDVRFARAGR
                     LFLSALKGVVDPEKKRKIIGRIFIEVFEAEAKKIKGAAFLAQGTLYPDVIESRSAFGG
                     PSAVIKSHHNVGGLPKRMRFRLIEPLKHLFKDEVRLLGRELGLPDDLVWRQPFPGPGL
                     AVRIVGEVTAGRLTILKKADALFDEEIRAAGLYLTLWQSFAVLLPIKSVGVMGDRRTY
                     EHIITLRAVTSDDAMTADWARLPHDLLSRVSNRIINEVRGVNRVVYDISSKPPSTIEW
                     E"
     gene            complement(30914..32059)
                     /gene="carA"
                     /locus_tag="PLW16_04050"
     CDS             complement(30914..32059)
                     /gene="carA"
                     /locus_tag="PLW16_04050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPB70110.1"
                     /translation="MSLLTKNRPALLVLEDGSLFRGSAFAGRGEVLGEVVFNTGMTGY
                     QEILTDPSYAGQIVAMTYPLIGNTGINPEDMESAGVHLEGFICREYQAVPSNWRSRET
                     LKAFLETHDKLGVEGIDTRALTRRLRLAGAMRGILTTETEDLDGLLQRVRSYPGLVGR
                     DLVKAVTCNRSYRWQDGRAVPLDGPLTAAARAEAARRRVVVLDCGVKYNILRHLERLG
                     CEVLVVPSRTAARDILACRPDGVLLSNGPGDPAALPDIVATVRELVGNVPLFGICLGH
                     QILGQALGGRTGKLKFGHHGINQPVLNRRTGRIEITSQNHGFVVIPESLPAGVERARD
                     NLNDRTSEGIALTARRAFSVQYHPEAAPGPHDATELFEAFVRVMEGG"
     gene            complement(32098..33429)
                     /locus_tag="PLW16_04055"
     CDS             complement(32098..33429)
                     /locus_tag="PLW16_04055"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPB70111.1"
                     /translation="MLETKTQKDVLKAVKDHKVSFIQFWFTDVLGMQKIFSITPDELE
                     DGLREGMGFDGSSIQGFCRIEESDMIAMPDPTTFQLIPWRPTDRPVARMFCDIQNPDG
                     TPYEGDPRYVLKRILKKVGDLGYRFLVGPELEFFYFADDTLPEPLDRGGYFDGLPIDR
                     ATDLRRQTIFALQEMGIRVEYSHHEVAPSQHEIDLRYSDALTMADAVMTYRTTVKEIA
                     RQNNVYATFMPKPIFGQNGSGMHTHQSLFKGGKNAFYDGRDAYNLSRMAKHYIAGLLV
                     HAPEITAVCNQWVNSYKRLVPGYEAPVYVSWARRNRSAMIRVPMYKPGKENATRMEFR
                     SPDPACNPYLAFAVMLAAGLKGVEKKYPLPEPVEEDIYEMDEKVRAAAGITSLPGSLH
                     QAIGLVEKSELVRETLGDHIFNKFVENKKLEWDRFRIHVSQFELERYLPIL"
     gene            complement(33457..35553)
                     /locus_tag="PLW16_04060"
     CDS             complement(33457..35553)
                     /locus_tag="PLW16_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPB70112.1"
                     /translation="MELTEVFGSNVFNDKVMKKRLPKPVYRMLKETIEQDLPLGPDVA
                     EVVANAMKDWALGKGATHYTHWFQPLTGITAEKHDSFISPTADGGIIMEFSGRQLIQG
                     EPDASSFPSGGLRSTFEARGYTAWDCTSPAILKTDMAGNVTLTIPTAFYSYYGEALDK
                     KTPLLRSMKALSKQALRVLRALGNTSSTKVCPTVGPEQEYFLVDGQFFQERIDLMLTG
                     RTLFGAPAPKGQELEDQYFGAIKDRVSAFMSDLNAELWKMGITAKTQHNEVAPAQYEM
                     APIFATTNIATDHNQLVMETLRKVALRHDLVCLLHEKPYAGINGSGKHNNWSLSTDDG
                     INLLEPGNTPGENVQFLLVLSALIKAVDTHADILRATCAHAGNDHRLGANEAPPAIIS
                     VFIGDELTDILEKLARGQKVSAKGAQFLRVGVDTLPEFPRDNTDRNRTSPFAFTGNKF
                     EFRMVGSAQSIAGTNVALNTIAAEVLDEIATRLEKAKDKNAEAATIIKEIYKKHHRVI
                     FNGNNYSEEWSKEAARRGLPNVTNAVDALKAFVTDKAIRLFERYGVLSSKELESRYEI
                     YVEIYAKQINIEALAAIDMVKKQFLSAATEYGTFLAESIASFKAAGVAAPVQTDLLKK
                     LGALLASAYKNLERLEAATARAQKLDDIVGKSEACRDKVIPAMQALRGDIDALEMIVP
                     GDLWPVPTYTDLLFKL"
     gene            complement(35640..37973)
                     /locus_tag="PLW16_04065"
     CDS             complement(35640..37973)
                     /locus_tag="PLW16_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70113.1"
                     /translation="MNGASKPTWVIPGMEKGLRVDSRVLEERIQKAVSDGHRSLEIIA
                     QGQHGIGGRLWRAGNETVRIRVMGTSGQRLGAMGFPNTVIDCVGPASDDVGWLNAGAR
                     IIVRGNATNGAGNAMAQGQIYVAGNIGARGMTMTKHNPRFAPPELWVLGGVGDSFAEF
                     MAGGHAVVCGHGVERERAGLGYRPCVGMVGGKIFFRGSCPGYSENDAVLAPVDDEDWR
                     WLIAGMKGFLAAIDQPGLYKTLTADRGQWQVLAARRTQEKHAGPYRSLPQFLREVWEA
                     QLGAGGLIGDLTDLDRSPVDVIAKGDLRRFVPVWENEKYLPPCQAHCPTGIPVQKRWA
                     LIREGKLSEAVDLALKYTPFPATVCGHLCPNLCMQHCTRRSVNLPAVDVTLLGRASLE
                     AAPPEPAPPTGKKIAVIGGGPAGLSVAWQLRLMGHEPVIHETDSRLGGKITETIPASR
                     IPDEVLEHELARVAGLIHHVRKPVTRKGFDELKKKYDFIVVAAGARKPRMIDVAGWER
                     AEAALDFLRRSKAGKARVGRTVVVIGAGNVGCDAAAEAARLGAESITLIDIQAPASFG
                     EEREHAEAAGAKFLWPRFTKSITKDAVELTTGEVLPAETVIVAVGDQPDLGFLPEGVA
                     RERGFIAVDARGRTSDPQVFAVGDAVRPGLLTEAIGAGRTAARAIDALIRGREETFDQ
                     LPPIEPERVKHAYFDAGANGFASVTACAAACASCGACRDCGLCEQICPEGAVARRDPG
                     DGTFTYEVDAARCIGCGFCVGACPTGVWRLTENEPLE"
     gene            complement(38015..39640)
                     /locus_tag="PLW16_04070"
     CDS             complement(38015..39640)
                     /locus_tag="PLW16_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPB70114.1"
                     /translation="MGRIDPATLSVRDLPWRVLWNRETCTLCGRCTAVCPVQAIELGV
                     HRKRVVTAAVGLADKPTQAVGLYHGIRQRTDAAHACVGCGMCALVCPNGSIAPAHGDE
                     TDKLRFHVQQGGEPRRRGGRRNDPQSVLDAIKFIRISMLTDPALDAGRHEFELRTLLG
                     RVLPPEEILRVTKENGWVPPVREIYPLVIGSMSFGALSPNMWEGLQMGVAYLNEEMGM
                     PVRICTGEGGCPPRLLRSRFLKYVILQIASGYFGWDEILHALPEMQEDPCAIEIKYGQ
                     GAKPGDGGLLMWYKVNRLIAAIRGVPAGVSLPSPPTHQTKYSIEEAVAKMIQSMAMAW
                     GFRVPVYPKISATSTTLAVLNNLTRNPYAGGLAIDGEDGGTGAAYNVSMDHMGHPIAS
                     NVRDAYLNLVKVGKQNEIPIFAGGGVGKNGNLAANAAALIMLGASGVQTGKYLMQAAA
                     GCLGSERDRCNICNIGLCPKGITSQDPRLYRRLDPEKVAERLVDVFKGFDTELKKIVA
                     PLGRSTSLPIGMSDAIGIADGHAAERLKIKYVV"
     gene            complement(39613..40743)
                     /locus_tag="PLW16_04075"
     CDS             complement(39613..40743)
                     /locus_tag="PLW16_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HPB70115.1"
                     /translation="MCRLFAITSAEPLSPMVAIRALDVMKEGHDGSGVGLFLTDLGGD
                     FEQFRGGPILSGIFSNAGIKALDRYMMDMDFMVKYKLSIRPPMAPPPGTPKRDNYLIR
                     VYEYPPEWEVLSQAELHHRLMMTRLELRRMGEVDGSMLIFSFWPDVIMIKEVGDPLTV
                     AEYLGLNRRELQARVVLAQGRQNTNYAIDIYACHPFFIQGIATMTNGENTAFVPIREF
                     LTSRRYEGYQGYQSDSEVFTHILHYTRNQLGLGLEMYKHIITPLKDDELAEHPNGEWL
                     RRVKQSCRPLIVDGPNCVMGCLPDKGLFMVQDGKKLRPGIVGGNPGMIAFSSEMCGLD
                     TAIPARDKSRDFQPMKYDTVYVGPDRQEVRQWSQWDELTPRP"
     gene            complement(41021..42619)
                     /locus_tag="PLW16_04080"
     CDS             complement(41021..42619)
                     /locus_tag="PLW16_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB70116.1"
                     /translation="MSREVWSPTQVGLAETKDISIHSYEELAALHAIARILVRPGELH
                     AELAGVLKEMSRLLGMQRGMISLIDRETGEAWLDVAHGVDIGGMEVTYRPGEGITGKV
                     AQTGRPMAVANLGQEALFLDRTGARRFLNRAELAFLCVPIIYDQRVVGVLSADKVARQ
                     VENLERELAVLVAVADLIAKAVHTRALEEDNRRLRHMLGKSCSPSDELIGTSGVMQEV
                     FGLMAQVAESDTTVLIHGATGTGKELVGRAIHRNSPRRKGPLVLVNCAAMPDTLVESE
                     LFGHEKGAFTGAWQRRRGRFEEADGGTIVLDEVGELSAAAQAKLLRVLQERQFQPLGA
                     SRTVRVDVRIIAATNRNLEQDVADGRFRADLYYRLNVFPLYLPLLKDRGSDIILLADH
                     FVEKYGRALGKLVKRISPAVMEILLAYGWPGNVRELENCIERAVVLCTGDTVETAHLP
                     PSLQSGRSEEERRERGKLKVEITARERALLMAALREAGGNQTRAAKLLGTTKRIVQYK
                     VHKLGIDTRQFRPAPPPALSTGET"
     gene            43217..44896
                     /locus_tag="PLW16_04085"
     CDS             43217..44896
                     /locus_tag="PLW16_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70117.1"
                     /translation="MSDVYTEKPWLKNYDKNVPPTLKYEDKTFAEMFRKAVEAFPDKT
                     ALHYMGKKLTFRDLDLLSNQLAQYLLKIGLKPDDVVGMHMPNIPAHYLGLIAVQKAGC
                     VSTGLSPLLTPHEMEHQLTDSRAKVVMTVDVLFEKVAEVAGKTPFSTVIVAEIADFLP
                     GVKRVLGKLLKKIPTAPIRPLPGKTVLKFMEAIRGMPTDRVAVKRTMDDTIFMMYTGG
                     TTGPSKGAILTQRGYMYNRLQTCAWLDIGSKDVALSAFPLFHIAGLALGGFTMTSGAT
                     QICVPNPRDSHFLIGAIKAYKPTMIVNVPTVFFELLKVPEFKALDLSNLNFCLSAAAP
                     FPAEYIAELEKIIGAGNFIELYGMTEMSPVMCCNPRYGKKKASSIGMPMPDTEFRLID
                     PETGQPAKLGEPGEIVARGPQLMKEYYEKPEETANAVRDGWMYTGDVAKMDEDGYFYL
                     VDRVKDMVIVSGFKVFTRELDDLLATHPDVDVAATIGVPDPARPGSERVAIAIVLKPG
                     VDKSEAEKEKLTKFLKENVAPYKVPKVIQFMDALPTSGVGKVLKRELRKMM"
     gene            45048..47102
                     /gene="tkt"
                     /locus_tag="PLW16_04090"
     CDS             45048..47102
                     /gene="tkt"
                     /locus_tag="PLW16_04090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB70118.1"
                     /translation="MDVTPQQIETCINAIRFLAADAIQKAKSGHPGMPLGAAPAAYAL
                     WMRHLKHNPADPAWMDRDRFVLSAGHASMLLYALLHLTGYALSLDDLKNFRQWDSLTP
                     GHPERGRVPGVEVTTGPLGQGISHAVGMAIAEAHLAAVYNRPEHTVIDHFTYVMASDG
                     DLMEGVTAEACALAGHLGLGKLICLYDDNRISLAGSTALTFTENIAGRFTACGWQVLR
                     VEEGGDWTAVDRALAEAKADLAHPTLIAVRTVIGYGAPCKAGTADCHGSPLGEDELKG
                     AQERLGWPQEPPFFVPDEVREQFRGALRTGQARQADWQARWDRYREAYPEAAAELLRA
                     QAGELPAGWESALPAFADPSKEVATRKASEAVLQALAPKLPELLGGSADLNPSVITWL
                     KGYGDFQRPDLPEEGVQGRMGGVWSYAGRNIHFGVREHAMAAIAGGIACHGGLIPYAS
                     TFFTFADYMRPSIRLAALMELGVIYVFTHDSIGVGEDGPTHQPVEQLMSLRAVPNLAV
                     IRPADAAETVEAWRLALLNRRGPTALILTRQGLPVLDRTELAPASGLRRGGYVLWEAS
                     AGQPDVILIGTGSEVKLALAAGRTLAAEGIRARVVSLPSWDLFDRQPADWRDAVLPPG
                     VTARVAVEAGIRLGWEHYTGLGGAIVGMDTFGASAPSPVLFEKFGITAERVVAEAKRV
                     LA"
     gene            47229..>47409
                     /locus_tag="PLW16_04095"
     CDS             47229..>47409
                     /locus_tag="PLW16_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70119.1"
                     /translation="MKPGKAGCRRAAERGGSRREPPDTRTTKEGQTMTRDEYKKIISL
                     AIEREIEAYEFYRLVS"
     assembly_gap    47410..47509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <47510..47935
                     /locus_tag="PLW16_04100"
     CDS             <47510..47935
                     /locus_tag="PLW16_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB70120.1"
                     /translation="SLAIEREIEAYEFYRLVSERAKDNNLKSIFGDLAKDEQGHRRLL
                     EGFLTKAPEKMKFSEAKDYKIADAVPTPPLTADLKPIEGIVISIKNELEAMQMYTQLA
                     NASTDAAQKNVFLQLAAMERGHKSRLEDIYTNMAFPEAW"
     gene            48316..48867
                     /locus_tag="PLW16_04105"
     CDS             48316..48867
                     /locus_tag="PLW16_04105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB70121.1"
                     /translation="MNPYKIAVIVGSLRKDSLNRKLAHAIMKLAPPDFTFRQVEIGNL
                     PLYNQDDDANQAESVKQMKREIREARGLLFVTPEYNRSIPGVLKNAIDQASRPHGQND
                     WAGKPAGILGVSIGVIGTAMAQQHLRNVLAFLDVRTLAQPEAFIHAKDGLFDASGNIG
                     TDSKEFLQDWMDRYVAWVKKHVD"
     gene            complement(48961..49176)
                     /locus_tag="PLW16_04110"
                     /pseudo
     CDS             complement(48961..49176)
                     /locus_tag="PLW16_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         7981 a        16195 c        16167 g         8678 t
ORIGIN      
        1 gaacggccgg gccggggccg gacgggccag atcgccgacc cccacatctg gctgtcgccc
       61 cgcctgatga agacccaggc gcagacggtc gcggccgcgc tgggacgcct cgatccggcg
      121 ggggcgaacg cgtacgaccg gaacttgcgg acgctcgcgg acgacctgga ccgcctcgac
      181 cggcggctgg ccgcgcggct cgggccctgg cggggaagcc gcttctacgt ctttcacccc
      241 gcgttcggct atttcggcga cgcctacggc ctgatccagg tccccgtgga gatcgagggc
      301 aaggagccga gcgcccggca gctcgcgcgc ctgatccggt ccgcccgcgg ggacggcgtg
      361 cgcatgatct tcgtgcagcc ccagtttgcc cgcaagaagg ccgacgcggt cgcccggcag
      421 atcggcgggg tctgtatccc gctcgatccc ctggcggccg attatccggg caacatggaa
      481 cgcatcgcga cggccctgga gaaggggctg gtgcattgac cgggaggatg gccatgcagg
      541 acacgcacga ccccggggcg gccgccgttc cggaagagcc cgtcatcgat cttaagggcg
      601 tcagcttcgc ctacgacgga ccgcccgtgc tcgaggacgt cacgttccag gtgcgcgcgc
      661 gcgattttct gtccatcgtc ggccccaacg ccggcgggaa gacgaccctg atcaagttga
      721 tcctgggcct gcttcagccg acgcgggggt cgatccggct cttcggggaa cccccggcga
      781 aggggcgccg ccgggtcggc tacatgccgc agcaggcggc gctctccatc catttccccg
      841 tcaccgtgat cgaggtggtg ctgatgggcc gcctcacggc cggccggaat ccggtatttt
      901 accgccgggc ggaccgcgcg gccggcgcgg aggccctcgc tcggatggga cttgccgacc
      961 tgaaggaccg cccattctcc gcgctctccg gcgggcagcg ccagcgcacc ctgatcgccc
     1021 gcgccctcgt ctccgctccg gacctgctgc tcctcgacga accgacggcc aacgtggacg
     1081 cggcccggga aaccgaattc tacgaacttc tggagcgcct gagcgcggac atcaccatcg
     1141 tcctcgtgac ccacgatctc ggcttcgtct cccgctacgt ccggagcgtc gcctgcgtca
     1201 accgccgcgt cctcgtccac cccacctcgg acatcaccgg cgagatgatc aaccagcttt
     1261 acggcagcga cgtccggatg atccgccatg accaccgctg tgcgccggag gggcatgcat
     1321 ggtccaattc ctgatggatc tcggtcagtt cggttttctc aggaatgcgc tggcggtggg
     1381 gcttctggca agcgtcgcct gcggaatcgt gggaacgtac gtggtcaccc ggcgcattac
     1441 cgcgatcgcc ggcagcatcg cgcactgcgt cctggggggg ctgggggtcg cccgctggct
     1501 gcagacggtc catcacctgg aggggctgca tcccctgtac ggcgccgtga tcgccgcgct
     1561 tctggcggcc gtcctgatcg gctgggtgag catccgggcg aaacggcacg aggacacggt
     1621 catcagcgcc atctgggcca tcggcatggc cgtcggcatc ctctttatct tcaagacccc
     1681 ggggtacaac gaggacctgc tgagctacct cttcggcaac atcctcatgg tgaccgacga
     1741 agacctctgg atggtcgccg ggctcgacgt cgtgatcgtc gccgtcagcc tcctgttcta
     1801 ccaccccttc ctcgccacct gcttcgacga ggagttcgcc cgcctgaggg gtgtgccagt
     1861 ggagcggtac tacatcctgc tcctgtgcct gacggccctc acggtggtcc tgctcatcac
     1921 cgtcgtcggg atcatcctgg tcatcgccct gctcacgctg ccggccgcga tcgccgggac
     1981 gttctcacgg aagctctggc acatgatgat cttggccgcc ctgacgaccg ccgcgctgac
     2041 cgcgggcggg ctcgcgctga gctacggccc cgacctaccg gcgggggcca ccaccatcgc
     2101 cctggcgggc gtcgcctacc tcgcggccgt cctgggcaag cgcctgttcg gccgccgtcg
     2161 cgccatcggg gcgtccgggg gctgccctcc gggtgaagaa ctttcttagc ggctgccccg
     2221 ccccgcccgg atcgccccgg accggccggc gagcgcttcc ctggtcactt cggcaggatg
     2281 gccttctggc ccgtcagcgt ctcgacgatc cggccgtatt tctcgtggat catggtccgg
     2341 tcgaacgccc agaattcatc gagcatcgcc gcaagctccg ccgtcgtgaa gtagtccatg
     2401 cacgggaaaa agaagtgctt gtcctccttt tcgatgtgtt tggggtagaa ctcggccagc
     2461 gtcgtaagcc ccgccgcgat ctccgcccgg gtgtccgccc cctggaggaa gcgctctttc
     2521 gcctccacca ggcggccgac ggtctggcgc gacatcacgt gttcggcgac gagttcgtcc
     2581 atgatccgtt tgtgctccgc ggcgaggggc tttttcgcga ggtcccggaa gaggatctcc
     2641 tcctccttgc cgtggtgggt ccggtcggca taggtccgga tgaagtccac gatgacgtcg
     2701 acaaagacgg ggttgacctc gcgccgttcc cggatgcgtt cgatctcgag tccggcggcg
     2761 ctcagggcct gttcgatcag gcggtgttcc cacatcaggg gtccgatcgg tttcatgggc
     2821 gttcctcctt ttttccttga gattcaaaac gggcggcctg agccgcgcct atcttcccgg
     2881 gtccggcgac acgagcgtcc ggcccagggc ctcggcctca cggagcaggg cttcgttttt
     2941 ccggatgtcc cccaggcccg gcgcgctgcc gtaaaccatg ccgacgatgg gcgacttcgt
     3001 ataggcgaag gcgtcctgga aagtccggag ggcgttgatg cagccggagg tcacggggtc
     3061 cgtgtcgccg taggtcatgg cgatggcgat ccgtttcccg gcgaaggggt tcgacgcata
     3121 cgccagcagg gcaaagcacc ggtccatgaa gagcttggtc tgggcggaga gggtgaacca
     3181 gtaaacgggg ctggcgatca cccaagcctc ggcctcgatg agcttcgggt acagggcctg
     3241 catgtcgtcg tcgacggcgc agccccggct tcggggtttc tggcaggcat agcaggcccg
     3301 acagggggcg atgttcaggc cgtggagata gaccgattcc accgttgccc cggcgctccg
     3361 ggcgccgcgg gcgatctgct ccgccagaat cgcgctgttg ccttttttcc tcggactgcc
     3421 gaggagcatc aataccttgg gggccgcttt gttgccggtc atgggaacac ctcctgtcaa
     3481 aaggatgaac gcgccggggg ataaatactc ggcattaccc gctcagacgg acccgcaggc
     3541 gtcgccgggg tcgcaccact cggcctccgg atcggcggaa atagcggcga ttttccgctc
     3601 gaagtcccgc cacatcgcct cgttcccgag gtcgcaactg tggccccaga agaagaaggc
     3661 gccaccccgg gcgcgcgccg cgtcgtagcg ggtccagaag tccgccgcga gggcatggca
     3721 ggcgggcctg aaaacgcggg gggccggaag gcccggctcc ccgccggcct ccccgtcctc
     3781 ccggatccat gcatacccgg ccgcccgcac cgcctggcgg acggcgtcgt tgacggcgtt
     3841 gaaggggtag cagaacccgc gcacgggttt ccggaacagg tcctcgagcc ggcgccggct
     3901 ttccgccacc tcctcccaca ggcgatccgg cggcaggtcc gtcagatacg ggtgcgtcag
     3961 cgcgtggctg gcgatctcga acccgtcgta gacgcgggcc agctcgggga gggcgagccg
     4021 ccagacctcc cgctcccccg cccgccagcc cagggaccgc tccggtccgt acagacccgg
     4081 attcagggcg aaggccgccc gcgccccgtg acgccgcagg aggtccgcga ggcggatgtc
     4141 gtccaccagc ccatcatccc agcattgcag caccttcatg gcgcaccccc cggcgcctcg
     4201 gtcttgcggg cccgataggg gattctattc cataattccg ggatttcatc aatcgaagaa
     4261 tcggcggagg gccattcccc gccggggcgc aaaaaaccgg atcccccctt ccgccccggc
     4321 caatttgggg tcgattaaat cccgatagtt tgttaaaaag ggccatcatg gatcctatca
     4381 aacaaggagg tcagccatgg atgccaagga aaaggcggtt ctcgacgtcc tgaaaaagga
     4441 agggaaaccg atgcggcccg gcgacatcgc cgaaaaggcc ggcctggaca aggccgcggt
     4501 cgccaagatc atcgacaaac tcaagaagga ggggaaggtc gcctcgccca agcggtgctt
     4561 ctatgcgccg gtgtagggcg cgggcggatt ccgggaggtc gtaggccatg ttcttctatc
     4621 tgtccaagat cctggatttt gtcctgctgc cggtcctctg gatcggcgtg gtcctgctcg
     4681 tcgccgcgct cacgaaacag cggcggcgaa agcagcgctg gctcgtcgcg ggactggccc
     4741 tgctgatcgt cctgaccaac cccttcctgg tccgcgtcgc cctgaaccag tgggagcggc
     4801 cctaccgaac ggcggcgatc caccagccgt acgattacgc catcgtcctg ggcgggttct
     4861 cctactggga cggacggtac gggcggctga ccttcggcga ggccagcgac cggctgatgc
     4921 aggccctgga cctttaccga agcgggaagg tccggaagct cgtgctctcc ggggggcccg
     4981 gcagcctgtt caacacggag gagcgcgaag ccgcctacct caaggcctat ctcctgcggt
     5041 ccggcattcc ggagcgggac ctcctgatgg agtcgaactc ccggaacacc catgagaacg
     5101 cggtgtttgt ggcgcgcctg ctcgagggac ggggcggatc ctccctcctc gtcacctcgg
     5161 ccctgcacat gcggcgggcc gaggcgtgct tcgcgaaggc ggggctcgcc gtgtcgacct
     5221 ggccgacgga tcgcctgacg ccgccggagg cgagggcgtt cgaaccggga tatctctttc
     5281 tgccctcctc gaccgccctg gatcattggg agcggctggt gcgcgagatc gtcggatacg
     5341 gcgcatacgc gctggcgggt tacgtttgag ggcggaaaat aaacaacaaa taagaggctt
     5401 ttcgcgtttt gcggacaaga aaaccccttc acgaggcatc cgtgaagggg tttcaggagg
     5461 gaactactac ttctttagcc gcgcggccgg ggccttaccc gaccatgtcc ttgatcaggg
     5521 tgatcacggg attcgtgaac agggcgatca ggatggcgta catggcgaag gcgcccacgg
     5581 cctccccggc atacatcttt tcgtatttca tcttgaggga ggtgatgatc aggccgccga
     5641 tgatgaggcc gcccagctgg aagtagggga attcgtcggc gccgccggcc gcgaattccg
     5701 cgaagccgaa cgttgccgtc atcaaaacca ccgccgccgc gaccaccagg gatcccaaag
     5761 tctttttcat gatcctgtcc tcctttcgga cgttttatcg ggtttgactt gagttaatga
     5821 aaatagcgtg ccaagcgcgg accgcaggcg cagaaaagtt ataaatgcat gaaaatgcat
     5881 gtattttcca aaaccagaat tgaccggggg ccgcgggatc tcttcccggg ggcctccgcc
     5941 ttgaacaaaa tgtcaaacgg cgttgaaagg aacaatcagg gaagcccccg gcacggatcg
     6001 gccccgccgc catgaatgcc ccggtcgaca cgctccccct gtatccccgg cttgacgaga
     6061 tcgcggaggc gatcgcgcgt tcggggccgc tcgtgctgca cgccgaaccg ggggcgggca
     6121 agacgacgct cgttcccggt cggctgctcg cccacgaggc gttcgcgggg cggaagatcc
     6181 tcctcctcca gccgcgccgg atcgccgccc gcgccgccgc cgagcggatc gccgccctcc
     6241 ggggagagcg tctgggggaa acggtcgggc tgcgcacccg gctggaaacg atcgccggac
     6301 ggacgacgcg cctggaggtc gtgaccgagg gcgtgctgac gcggatcctc cagaacgacc
     6361 cgtcgctggc gccttatcag ctcgtcatct tcgacgaatt ccacgagcgc accctccagg
     6421 gggaccttgc cctggccttc tgctgggagg cccgccgggt cttccgccgc gacctcaagg
     6481 tgctgttcat gtccgccaca ccgccgacgg aggacctcca ggcggcgctc ggggcgctcg
     6541 aggtccgctc cgtcgccggc cgttcctggc ccgtcgaggt gctcgaccgg ccgccgcttg
     6601 cgggggaaac gccctgggca ggcgcggcgc gtctggctct ggaggcgcgg gaccgcctcg
     6661 accggacggg cggcggcgat gtcctggtct tcctgccggg cttccgcgag atccggctcg
     6721 cgcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgcg gcagcgccag
     6841 ggccgggccg gtcggctggg gccggggatc tgcctgcgct ggtggcgctc cgacgccccg
     6901 cggcggccgt tctcgccgcc ggagatcctg gaggcggacc tcgccccgct cctgctcgaa
     6961 acggccctct ggggggcggt ctccccgctc aacctgacct ggatcacgcc gccgcccgcc
     7021 ggcgccctcc agcaggccgg aacccttctg aaggcgctgg gactgatcga cggggccggg
     7081 cggatcaccg cctccggacg gcgggcggcc gggctgggtc tgcacccgcg cctggcctgc
     7141 atggtcctga atgccctggc gcggggatgg ctgcccacgg ccgccgtgac cgccgccctg
     7201 ctggaggagg gggacccgct gggcggggac gatccggatt tccgcgaccg gcttgcggcc
     7261 tgggccgccc gggcgcgggg ggtaaaaagc gccttgcggc cggacgtcgc gcaacggatc
     7321 ggccgggagg cggaacgcat cctacgggcg gcgggtcccg gggccggcgt cctccgcgcg
     7381 gcggagatcg atccggacct cgccggccgc ctcctgctgt tcgcttatcc ggaccgcgcc
     7441 gcccagcgcg tcggtggcgg ccatctgggc gaggccgccc gcctcgtcct ggccaccggg
     7501 cggggggcgc gcgtaaccgg tcccctcgcg cgggaggcgt tcctcgtcgc ggcggatctg
     7561 gacggagggg agacggaggc gcgggtcttt ctggccgccc cggtgacccg cggggaaatc
     7621 gaggggggac tcgccgggca gccggagacg gtgcatctgt ttgcctggga gggatgggtg
     7681 ccccgctccc gcacggaggt ccgcgtgggg cggatcgtgc tcgtggaggc gcgcggcgcc
     7741 gcggccccgc cggaggcgct tcggcaggcc gcgcgcgagc ggatcggccg ggacggtctg
     7801 gacggcctgc cctggtcgga ggcgtcccgg cgcctgctcg cccgctgccg cttcgtggag
     7861 gcccgcggcc gccgggaggg gtggcccggt ttttcggaca ccgtcctgct cgcggaggtc
     7921 gaggggtggc tcctgccgtt cggccgctgg gacggcggcg cggtcttttc gccggaggcg
     7981 ctgctacacg ccctggacgg ccgcctcggc ggcaaaaacc ggcgcgccct cgaggcgctc
     8041 gcccccgaat tcctggcgct cccgtcgggg acccgcaggc ggatcgacta tgaatccggc
     8101 gccgtcccgg tcgttgccgc ccgcctgcag gagtttttcg ggtgccgcga gacgccgcgc
     8161 ctctgcggcg agccggtcct gctccacctg ctctccccgg ccgggcgccc cgtgcagatc
     8221 acccgggacc tcgacgggtt ctgggagcgc gcctatcccg aggtgaagcg ggagctgatg
     8281 ggccgctacc cgcgtcaccc ctggccggac gatccccggt cggccccgcc cacggcgcgg
     8341 acaaaaagaa aatgaatgca gaaacggcgg gcgggcacgg gcggactcgg ctcccaacgt
     8401 cccgcccggc ggcggcgccg cacggcgtcg tcaggcccgc aggcgcgcgc gcaggcgcgt
     8461 atacctcgcc tcggtccggt tgttcccgat cgcgatggcc agcgcctttt tcgtcccgat
     8521 gaggacgacg agtttcttcc cccgggtgac cccggtgtag agaagattcc gctggagcag
     8581 gacgtagtgc tgcgtcagga tggggatgac cacggccgga tactcggagc cctgggattt
     8641 gtggatggag accgcgtagg cgtgtgcaag ctcgtccagc tccgagaagt cgtaaaggat
     8701 ctcccggccg tcgattgaga ccgtcagttc ctgcgcctcc cggtcgatcc cggagatccg
     8761 gccgatgtcc ccgttgaaga tctccttgtc gtagttgttg gaaatttgca tcaccttgtc
     8821 gcccgcccgg aagagccgtc ccatccggct gatgccttcc tcgccggggt tgagcgcctt
     8881 ctggagttcc gcgttcaggt tgatcccccc cacgatcccg cggtgcatcg gggtcaggac
     8941 ctggacgtcg tcgatgggat cgaggccgaa gcgccgggga atccgctcct tcaccagggt
     9001 gatgatcagc tcgagcacgc gcccggggtc ctcctgttcg atgaagtaga aatcgtcgag
     9061 ccgctcccgg gaagtcgcca gcgcgggcat ctgccccgcg ttgatgcggt gggcgttgac
     9121 gacgatcgaa ctctcgccgg cctggcggaa gatctcgttc agctcgatga ccgggacgac
     9181 cccggcgtcg atgatgtcct tcaggacgtt ccccgcgccg acggagggga gctggttgac
     9241 gtcgcccacc aggacgaagg tcgcccgggg cggcacggcc ttgagcaggt agtgcatcag
     9301 gagggtgtcg atcatcgagg cctcgtcgat gatgacgagg tcgccgtcga gcggcgcatc
     9361 ggcgttgcgc agaaacccgc ccgtctggaa gctgtattcg aggaggcggt ggatcgttct
     9421 ggcctcgtgg cccgtggcct cggtcatccg cttcgcggcc cggccggtcg gggcggcgag
     9481 caggatcttc ttcttaagcc ccgcgaagac gcggatcatc gcgttgatga tcgtcgtctt
     9541 ccccgtcccg ggtccgcccg tgacgaccag gaccttgtgg ccggcgacgg cgcggatcgc
     9601 ctcgacctgc ttttcggcca ggacgatgcc gagcttcgcc tcgagccagg ggatcgcctt
     9661 ctccgcatcg atctcccgca cggcccgcgg cgtctcgacc agggtccgga ggtggcgggc
     9721 gagcagggtc tcggcgagga aatacccggc caggtagacg gccgggtcgg tttcccgggc
     9781 gtccgccgga ccggggtccg gggcctccag gatgatccgc ctgtccgcgg cgaggcgcgc
     9841 cagcgcccgg tcgaggatct cccggtcgat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn tccgcctgtc cgcggcgagg cgcgccagcg cccggtcgag gatctcccgg
    10021 tcgattgcga gcatctcgcg gcaccgctcg atcaggggct cgcgggggta gtagacgtgg
    10081 ccctcttcgg tcaggacgtg caggacgtag aagagccccg cctccgcgcg gaattcggag
    10141 tcccgggcga agccgagctt ctccgcgatc cgatcggcgg tgaggaaacc gatcccgaag
    10201 acatccgtcg ccaggcggta cgggttctcc cggacaacgg cgatcgcgtc gttcccgtag
    10261 tgcctgaaga tccgggcggc gtaaccggcc ccaaccccgt ggtcctgcag gaagatcatg
    10321 atggcgcgga tttccttctg ctcgtcccac gccttccgga tcatcgcgat ccgcttgggg
    10381 ccgatcccct cgatctcggc cagccggccg atcgcggttt cgatgacgtg gagggtctcg
    10441 cccttgaacc gccggacgat ccgtttggcc atgaccgggc cgatcccccg gatcagtccc
    10501 gagcccaggt acttctcgag cccggacccc gtggcgggaa cgccgctttc gcaggagacg
    10561 atccggaact gctccccgta cttcgggtgg cggccccact cgccccgcat cgagaggacc
    10621 tcgcccgggg tcggattgac gatgtgcccg atgacggtga cgaggtcctt gcggccgggg
    10681 accttgaccc gggcaacggt atagccgttt tcctcgttgg cgtaggtgat ccgttcgatc
    10741 tgtccgcgga gttccgccaa gggttcattc gccatgggcc atcccttccg gtcccgggtg
    10801 ggcgtcgacc ggcgtccggc gcgcgcagcg ccactttagg ccactcccgg agcgaatgca
    10861 aagggtttca cgccccggcg ccaccgctca ccccttcgtc cggatcggcc ggcgccgctg
    10921 tcaagcggcg tccccgcgtg cctcccggcg gagcagtttc ttgtccagct tcccgacggg
    10981 ggtgagcggc agctccttcc tgaattccac cgtcttcggc acctcgtagg gggcaagctt
    11041 cgccctggcg aatgcgagga tgtcttccgt cagggccgcc tcgtccccct caaaggcatg
    11101 ccccgggtcc cgctggacaa acgccttgac gagctccgat ccgggccgtt ccggattggg
    11161 gatgccgatc agggccacgc tcgcgatcgc cgggtgctcc gccaggatcg cctcgacctt
    11221 gaccgaaaag accttgaagc cgctgacgat gatcatgtcc ttggtccggt cgacgacccg
    11281 gaggtagcct tcctcgtcct ggacgacgac atcgccggtg tgcaggtagc cgtcggcgtc
    11341 gatcacgttg cgggtctcct ccggtttgcc gtaataaccg accatcacct gcgggccctt
    11401 cacgcacatt tcccccggct cgccgaccgc gacctcccgg cccgtttccg gatcgagcag
    11461 ccggatgtcc gtattcaaca ggggcaggcc gatcgtcccc agctttttct tgcccttcac
    11521 cggattcgac acgatgagcg gcgacgtttc ggacatgccg taattctcca gcagcttccc
    11581 cttgccgacg atcttttcaa actccctctg ggcctcttca gggaaggggg aggccgaaga
    11641 gatgcacgtt tccacatgcg aatggtccat ctgccggaac ttcggattcg ccatgagcag
    11701 ttgatacagc gccggcacat tcaccaggag cgagggtttg tacttttcca gctcggcgca
    11761 gatgtgttcc gtatcgcggg ggttgggaat gagaatctgc agcccgccca gatagatgcc
    11821 gttggcgttc acgaagatgc cggcgatgtg gaagaagggg aacccgcaga gcgccgtcac
    11881 ctcgccctcc ttccagccca gccaggtctg gtagatgagg aggtcggcga cgcaattccg
    11941 atggctgagc atcgccccct tgggcatgcc ggtcgtcccg ccggtgtact gcagataggc
    12001 gatgtcgtcc ggcgtgatct cgactcccgg ctcgtccgtt gagtatttcc ccccttccag
    12061 gatttcctcg aagggggaca ccgtccgtcc cgcgatcggc gtgaccttgc cctccagacc
    12121 cgggaggaaa ctccccgccc tggccgtgac gatcaaacgc agggcctcga gctgggccgt
    12181 gagctccggc accttgtcgg cgaaggtgat gtccagggtc acgaagcctt tggcgttgct
    12241 gtcgcggagc tgataggcca cctgcgccgt cgtcaggagc ggtgagacgc ccgagacgac
    12301 gcaccccgcc ttgagcgtcc ccagccaggc gaagagatat tcgggcatgt tgggcaggct
    12361 gatccccacg acgtcgccct tctgaaatcc ctcggtcaaa agcatgtggg cgaagcggtt
    12421 ggccgcgcgg tcgagctcgg cgtagctgac ttgccggccg aaatacgcac aggccggttt
    12481 ctcgggaaat ttcctgaaga tggggcgaac cagctcggta taggtcgttt cgaaccgggc
    12541 cggatcgagg tccgtcagcc cttcatccca gtgcttcttc cagaatcgct ctgccttcat
    12601 gcgccccccc ttcacgttgt ggatcgcgaa gcgacccgtt gggaaacaag attgaacgac
    12661 ccgggcggat atgcttccgg atgcggcgcc ccctctctca ggcccccttt tgccgggtga
    12721 tttcctcgtc gcgcagggcg cggcgcagga ttttccccac gctcgtctgg ggcaattccg
    12781 tccggaattc gacggatttc ggaaccttgt aggccgcgag cttctcccgg cagaacgcca
    12841 cgatctcttc ctcggtctcg gtctggcccg ccttcagcac gacgaaggcc ttgaccgttt
    12901 cgccccggta cgcatcgggg atcccgacga cgcaggccgc ctgcaccttg gggtgctcgt
    12961 agaggatctc gtccacctcg cgggggtaga tgttgtagcc gcccgcgatg atcacgtcct
    13021 tcttccggtc cacgatgaag aggtacccct cgtcgtcgag gtaaccgatg tctcccgtgg
    13081 ccagccagcc gtcccggatc gaagcggtcg cctccggatc gttgtagtat ccccgggtca
    13141 cctggggacc cttgatcagg atctcgccgg tttcgcccag cttcaaatcc cgggtgccgg
    13201 tatcgagatc gacgatgcgg cagtccgtct ccggcacggg aacgcccacg ctgcccagct
    13261 tcgtcttccc gcgccaggga ttgaacgtcg ccagcgccga ggtctccgtc agaccgtaga
    13321 gctccacgat ggtcgagccg gtcgcctcct cccacgcgcg gatggtctcc agggccaccg
    13381 gtgctgcgcc ggagaggcac cccttgatga agtcgagctt ggccgacttg aatttcgggt
    13441 tgttcaggag gcccacgaac atcgtgggaa ccccgccgaa cagaccgggc cggtatttca
    13501 gggtcagcgc gatgatcacg tccggcgtgg gacggggcac gagcacggcg gtccagcccc
    13561 ggtagatggc gttgttcatg atgtccgtgt agccggcgac gtggaaaaac ggcagcacgg
    13621 cgagcagccg ttccatcccg tcctcggcgt cgaagatcca ttccttgaac tgctgcacga
    13681 ccgtgctgga gttcgcatgg ctgatcacga ccgccttgga caccccggtc gtccccccgg
    13741 tgaagatgaa ggccgccgga tcgtcgaagg ccacccgggt ttccggcggc gtcccgcccc
    13801 cctccaggag ggcgcggaac tcgtagacgc cttcctgcgg cgaaacgtcc atgtgcaggg
    13861 cgtaaagctc ctgaacgaag gggtcgtttc cgggatcggg aaggtactcc cggatgtggc
    13921 aggcgacgac cgcctcgagg cgcgtcctgt ctttcaggga aagaacccgc ggcaccaaaa
    13981 aatcaaggca gacggccatg cgggcctcgg attggcggag gttatgctcc agttcggtct
    14041 ccgtatacag gggattgttc atcaccgcca cggcccccag acggaacagg gcgtaggtgg
    14101 cgatgaccat ctgcggcatg ttcgccagga tgagcgcgac cttgtccccc ttccggatcc
    14161 cgagaccggc cagggcgccg gcaaaccggt ccacattcgc atgaagctga cggtaggtga
    14221 tttcggtccc catcatgatc agggcgacct gttccgggta tcgtgcggcc gtccgcgcaa
    14281 ggatttcgta cagggtgatc cgctcgatat cgagcgtcgg tgaaacgccg gcgacatagg
    14341 ccttgtgcca gatcctctcc tccatggggt gtttccctcc ttctttattt gatgggcgtg
    14401 cggacggggc gcgtttcctc ctccgttttt tcaggtatcg ttcgacggcg tgttcgccac
    14461 aggtctgccg tccgcggccg tcgcagcgat gacgaggcgc tcggccagct ccagggtcgg
    14521 cttcaggaag gggctctcgc cctttccccc gcccttgctg tcctccagct ggatgacgcc
    14581 cacgaagagc gcccagacca cgtcgttgaa cgggtggacg tccatccggc ggaggagccc
    14641 ctggtccatg gcggcgctca ggatgcgcct cccctgttcg aacgtgaggc gcgccttggc
    14701 cacgaactcc cgccggacgt cttcgtcgag cacgcccacc atgccggagt gctggaagag
    14761 ctgaaggatc cggaaggcct cggggtcggt ccagtagaca ccgtacaggg cctggaaggt
    14821 gtcgtgaagc agcgccgcgc cggtcgggta gccgcccgac atcagatggc gctcgatccg
    14881 ttccagggcc tgctcggttt tttccgaggc gggggccaga agcgagaaga aaaggtcgtc
    14941 tttcgatttg aagtattgat agatggtggg cttgctgacg cgcacctcga gggcgacgtc
    15001 gttcatcgtc atcttctgat agcccttctg gaagaacagg cgcttggcgg cgtcgaggat
    15061 gtcgcggata cgccgttccc gctcctgccg cttgcgctcc cggatggctt cgcgatgcga
    15121 gtctgccttg cccatgcccc gtcccttcgg atgacggaca gccgacgccc taccgttggt
    15181 aaaaaatata ctcccggtat cgtcctgtca agcgttttcc gatcccctgc cggcgcgcgg
    15241 cgagcccggc gaggaaaaac ggaggtgcgt ttgccccgcg ttttgtcgtt ggttcaggcg
    15301 gcgcggcgtg gtatagtgca gccgtccgtc catggaaatc cgcctgcaaa aaagaagtcg
    15361 tcgcgccgtt gtcgacgaag cgccccgaac ccgttgaatc aggagggaga catgatcgag
    15421 aatctgagtc gcgaacagtt ggcggccatt ctggacgccc tgcccatcga gttcatcttc
    15481 atcgacaccc aggagaagct cctgtacgcg aacaagggcg aaaggcggtc tcggccggcg
    15541 cccaccgacg tcgtcggcaa ggacatccgc ggctgccacc agccggcgag ccttcccctc
    15601 gtggagcggt tcgtcgacaa cctcaagagc ggccggaaag acgaagagga attctggctg
    15661 ctctttcccg accggaagat cctgaaccgt ttcctcgccg tccgggacaa ggcgggccgg
    15721 tatctcggga tgatcgagta tctcctcgat ttcaaggccc tggaggacct ggcggaggac
    15781 aggaagggcg cccacgcgcg cgactactcc accccgccgc cgagcggcat cccggtgacc
    15841 tgagcgttcg tgacggcggg agcggagtca tccaaaacct cagcccccgg actcatacgc
    15901 cggacggatg attcccgaag tgggggagga atctccgccg ggcagacgag gggatcgacc
    15961 agacaacaaa acctggagga gggacacatg atggagaaga cggcactggc gaagcagggc
    16021 atccggtcca acgacacggg ccggccggcc tacgatctcg tgatcatcgg cgccgggatc
    16081 agcgggttgt cgacggggct catgtggctc aagaacacgc agggcaagaa gaccctgatc
    16141 gtcgagaaga acccctaccc ggggggttac gtcacggcct acgagcggaa cggctacgtg
    16201 ttcgaaacga cgcagctctt tccggacatc gtgaacatcc tcgactacct tggcctggag
    16261 gtcccgctca agccctttcc cgggaccttc atgcgacgcc tcgtcgtcca cggcaacggg
    16321 gtgacggagt accgaatccc cgccggcgtc gaaaacttca cggtgtacct ctgcgaacaa
    16381 tttccggggg acgccgccaa gatccggaaa ctgatggcct attccgtgga gatgttcgcc
    16441 caggtccgga aactcaaggc cctgccgacc ctcggggaca tgctcaccac ccccttcgcg
    16501 gcaccgaagg tggtcgccaa tctgaaccgc acgtacgccg gcctgctcga ccgcttccgc
    16561 atcaccgacg accggctgcg ggaagttctg gaaaccttca gctccttctc cggcgtgccg
    16621 tccgcccggg cttccgccat tctcaccgcc ggggcgatgc tgtcctccat ggcccgctgc
    16681 taccggccgt acggattctt cgacaccttt cccgcggcga tggcccggcg ctttcaggaa
    16741 ctgggtgggg agatccggct gcgggccgag gtggagcgga tcgtcgtgga aaacggacgg
    16801 gtcgccgggg ttcgggtcaa gggagacgaa ccggtgatcc gggcggagcg ggtcgtcacg
    16861 acggtggacc cgatggtggc catgcgccgc ctcgtggggg aggaacacct gccccccaag
    16921 tatctcgacc gtctgaaggg gatcgtcatg tccccttcat cgctcaatgt ggctctgggc
    16981 ctcgacgacg gcatcgacct ggcccgcctc gatctcgact atccctacaa cgtcgtttcg
    17041 acgggacggg gcacgacgga acggctcttc gacgcctttc tcgagggcgg caacgccttc
    17101 tccccggaat gtttccacat ggccgtcatc tgtccctcgc tcaccacggg cgcaaagaac
    17161 accgtaacgc tccgcggcgt tcccttcggg atcggcgact ggctgtcgtg gcggcagaaa
    17221 gacccgggcc gctaccaggc ggagaaggag cggtgggcgg agttcttcat cggcatcgcg
    17281 gagaagtact tcatccccga cctgagccga cacatcgtgg tcaaggatat cgccaccccg
    17341 gcgacgtacg cccgctactc cgggtcgccc acgggcagca tctacgacat ggcggcgctc
    17401 gtcacgcagt tcgggccgaa gcgcctgccc atgaagacgc cggtggcgaa cctctaccag
    17461 cccaagttcg cccacggcct gtacggcggg atgatgaacg gggtgcaggt ggtcgatctg
    17521 atgctcgacc gggcgttcaa tggcggcaac tccctgttca acccgcaggg acgtccggcc
    17581 gcctgatgcc cgtggcggaa ggcgcgggct tcggcaggag gtacagcagg cccgggagga
    17641 gcacgatcat gatcggccgg tcggcgttcc ggaaccgggc ccgggcgatc accctgccgc
    17701 cgggtccgaa ggccgggtcg accgctccag cctcagcgtg acccggttcg cgctgtccgg
    17761 gcagggcacc tcgatcgtgt tcccctccca ccagggaaga ctcccgccga actggaaggt
    17821 ggacagggcg ggaaagaggt cgtgatagaa gaatccgcat aacccgccgg gattgtggca
    17881 actgatctcg aacgtgtccc ccgttcgatg gccggcgctg cactcccctc ttacgtccgt
    17941 aacggtggcg acgatgcgat aaccaatccc cgaatccgtg gccatacccg caacctcctt
    18001 atgggatcat tcccactgaa aaggccagca tgaactgggc cgcataatac atcggcagac
    18061 ccgcataccg gttggaaatg ctttttttca caaaccggtg cctggccaca aacaggtctg
    18121 aaacatagaa gaagatcgac ccggtcatga caagggcccg ggcttgaacg cagtagtccg
    18181 ggcggacggc cagggaggcc gctcccacaa tcatgaggga gatgatggca acataggcga
    18241 tcacgggacc cctcatggaa ccgaggtgcg ggcgcagcct gacgaagacg tatgaggaga
    18301 tgatcgaaac cgccgcgacg gacccccatg tcccggcgtt gatcccggcg gcgatgaaaa
    18361 acgccacgat gtaggccaga tgtcccgtga ggaaaaacgc aagtccggcg gtaaataccc
    18421 tgcggttaaa gaagaatgcc agacagatgt cgccggcgaa gcagaaggca agacctgtca
    18481 ggatgagata aaaatagggc cccgcatgat ggggctgggc cagggccgtt gcgatgaaca
    18541 gtcccgacag aagcggtttc gtcaggagga tgcccttcag gctttctttt ttttccgcca
    18601 aaagaaggcc ccccagaaga acaacggcga accccaggat aatgatgatt gccatggttt
    18661 tccctccatc ggtcccgcgt cgcccgttgg gtgaccggcg cggtgcttcc cccttgcgtc
    18721 cgtgacggtg gcgacgatgt taaccgatcc ccggatccgt gaccgcaccc atccccaccc
    18781 tggatcggcg cggcctttcg gaggatgcgc cggacgcccg ggtgcgctca ccggatccct
    18841 ttccccgtgg accggtcggt tcattcgggg tcctggaact ggggcgcccg tttggcgaag
    18901 ttggccgtga tggcctccaa ttgattgggg gttgtaatca gggtcatctc cagctcggac
    18961 tccatcaaca gtccccgggc cgcatccccg tgccagacct cgttcaggag cttcttggcg
    19021 gcgcggacgg cgtgcgggtt cttggcggcg atctcgcggg ccaggtcgat ggcctcgtcg
    19081 taaggcttgt cgcagacccg ggtgaccagc ctcagctggg cggcctcctc cccccggatg
    19141 accttccccg tgaagatcag ctccttggcc acgtcgaggg agacgaggtc gcgaacggtc
    19201 tgggaagcgc tcatgtccgg gatcaggccc catttcatct ccatgatgga gagtttggcg
    19261 tcgggcgtgc aatagcggat gtcggccccc agggcgatct ggcagccgcc gccgagcgca
    19321 accccgtgaa tggcggcgat caccggcatc gggagctgtt tccacccgta ggcgatgtgc
    19381 tggaagatgt tcgtgattcg gttcccgtag cggtcggtga ggcggggcag ttgcgcgcct
    19441 tcccccgact gcatcgccgc aaaattctcg aaatccaggc cggcacagaa tccctttccc
    19501 tcgcccgaaa ggacaacggc ccggacggcc gcctcgccgg ccaggcgatc gatcgtgtcg
    19561 gcgatggcct ggaccattgc cagactcagg gcgttgtact tgtcgggccg gttgaagcgg
    19621 acgtccgcca cgtggttttc gatcgaaacg gttaccagtt gttcagtcat gggtcctcct
    19681 ggaaatgggt tcgtgggttg tcgtccggac ggcccccggc gggcgggagc atccgcttca
    19741 cagcgcctcg atccggcagg gcacgtagcg gtgcaggggc gttcccagcc agtcgcggtt
    19801 cgtgtttttc gtcagacggt tgacgttcgc gccgtggacc ttcccgtcgt aaaccatccc
    19861 gaagccgtgg gggatcacga catgaccctt gcgggccgtc tccgtcacct ccgcctcgat
    19921 ctccacggaa ccggcctccg tcgtgacccg caccgtctgt ccgtccttca cgttcagccc
    19981 ctcggcgtcg gcgggatgca ataaaacggt gcagtcgcgc ttccccgcgt tccaggcggg
    20041 attgcgcatc agggtgttgg cgttcatgtt gaagtgccgt ccggccacga gcacgaaggg
    20101 gaaggccggg tccgggacga gggccgcctc ctcgtgcgcc gcgtcgaggg tttccagctc
    20161 ggcccggagc tccggaatca ccaggttgat ccggccgtcc tccgtccgga tttcgtcaag
    20221 gttccgatcc ggatcgagcc ggccgatcca gatcccctcg ggatggtcca gaatcttctg
    20281 gtagagctcc tctcccatgc ccatgccggg cgtgaatccc gcgcgggcgg cattcttgcg
    20341 gaggggtttg ggggccgtct gcagcatccc ccacagggcg gccaggtgca cggacccctt
    20401 ggcccggccc agggtcttgc cgaggacgaa cggcatggcc gccagggccg ccggttcctg
    20461 ggcggcatag tccatcagga cctgaccgaa ggcggcgtgg ccttcttcgg ccgccttgta
    20521 aaggctttcc gggatgggcg gaatgaggcc cagtcggtcc gccaggcgca gatggatctc
    20581 ccccagctcc aggggctctc cgtccggctc gatgatgggc cgtcgcatct ggaagtagat
    20641 gcccggaaag gtcatgggga aaaacgtccc gtcccacgat tcgtagcctg accgggacgg
    20701 caggacgtag tgggacagga ccgccgtctc cgtcagggcg agctccgccg tcacgaggag
    20761 gtccaggcgc ttgaaggccg tttcgtaggc cgtcgtgtcc gcgtaggaac gcaggggatg
    20821 cgccccggac acgatcaccg cccggaggcg ctcggggtgg tcggagagga tctcctcggg
    20881 catggcgttg ggcgggaaac aacccaggac ggggaaaaag ttcgtggtaa cggtccgcca
    20941 ggtcttgggg tcccgttcgt ccgagtggct ccccaggggc atcatctggc cgtggaagac
    21001 gttgcccccg cggacgccga tccggccgca gatcgcctgg agcaccacgg ccagatagga
    21061 tgaaaccgcg ctgtggcgat tcatcaaaag ccccaggtcg tagcgcaggc aggacttgcg
    21121 cgtggcgaac tgacggcaga cctcgtggac ttggtcgaag tcgaggttgc aggtgcggac
    21181 ggcggcgcgc gcgtcgaagt ccgtaaaaag gccccggatg gcatcgaggc cggaggtgtg
    21241 atgggcgatg tagtccctgt tctcccatcc ctcccggagg atgatggcga tcatggcccg
    21301 caggaggagg gcgtccgtcc ccgggcggat ggcgagatgg aggttggcga ccttggccgt
    21361 ctccgacagg cgcggatcga tgacgacgag ggatttgtcg gggtccttgg cgatccgcag
    21421 gaggtgtcgc ggggcctggg ggatctggtg gctctgcatc ccgttccagc cgaagacgag
    21481 caggaaatcc gtctcctccg cgtcgatctc ggcaaggagc gtctggcggc cgtgcatccg
    21541 gccgttggcc cagaacatgc cggagaactc ctgggccagg gcgctgtagt ggtagcggga
    21601 gccgagcccc cgcagcaggc ggacaccaaa ggccgcctcg aagtggcacc cctggccgcc
    21661 gccccccata taggcgaagg agcgggggcc gtaccggtcg acgatctccc ggatcttccc
    21721 tccgatctcg tcgatggcct gctcccacga gatccgctcg aatcgatctc cgacgcgctt
    21781 caacggatac ttcaggcgat cggcgttgtg ctggtggttg gcgatgttca gccccttgcg
    21841 gcagacgtac ccctcgctgc ggacgttgcc cttgtcgccc cgcaccttca cgatgcggtt
    21901 gttctcgacc tcgacctcca ggccgcagtt ctgggggcag aggacacagc cggtccggtg
    21961 gatcgttccc atggtttttc ctttctgtgc tgccggatga tgccgcaatc aagcgcggcc
    22021 gctctgatcc ctgagcttcc ggatcaggcc ccgcaactcc ttccgttcgc gttccgtcag
    22081 gatgtccaga aattcacggt tggcctcgac gatcgaccgg accgcttcag cggccagggt
    22141 ccgccccggg tccgtcaggt ggatctggat ggaccgccgg tcggcgggat gaacgcggcg
    22201 ctcgaggagc cgggccgcct ccagtcggtc gaggaggccc gtcatggttg cactgtccag
    22261 ctccgtccgc ttgcccagtt cgctggaggt gattccgtct tcatcggcga gaaagccgag
    22321 caccagggcc tggacgggcg tgatgttcag cgcggcgacc ttttgggcca ggaaacggct
    22381 cccgagctga tgggccttcg ccagctgaaa gaaaatgcag tcttccggtt tcacgcgtct
    22441 gccccctatt gctcgcctgc gaactacttt catgcgagca tattccagga ccgcgcgctt
    22501 gtcaatggaa caattctgtc cggttggatg cggaatggac aaatccgtct ccggtcgaaa
    22561 cccccgccgc ccattcaacc gcttgaattt gaatgacaat ttttatcgtg caggttggca
    22621 ggcgaattgc tcttctcccg gccgtatcca ttattcctgg gaggagacgc ggcatgatga
    22681 acccgatcga tacaggcgac acggcctgga tcctggtatg ttgttcgctg gtgctgttga
    22741 tgacgccgtc cctggcgctc ttttacgggg gcatggtgcg ccgaaagaac gtgttgtcca
    22801 cgctgaccct gagctatatc ttcatggccg tgatcggcgt ccagtgggtc ctgtacggat
    22861 actccctggc cttcggcgcc gatatcgggg ggctcatcgg cggcctgaat tatctcggac
    22921 tcaccggggt cgatgcgctg cccaacgaaa actacgcccg gacccttccc cacggactct
    22981 tcgccgcgtt ccagatgatg ttcgcggtca tcaccccggc gctgatcacc ggggccttcg
    23041 tcgagcgcgt caaattcaag gcgttcctgc tgttcagtct cctttgggcg accctggtct
    23101 acgatccgct ctgccactgg gtctggggtc agggcggctg gctgaagcag atgggggtcc
    23161 tggacttcgc cgggggcacg gtcgtgcata tcgccgccgg cttttcggcc ctggcctttg
    23221 ccctggtgat cgggccgcgg aaggggttcg gcaggagccc catggagccc ggcaacatcc
    23281 ccctgaccgt catcggcgcc ggattgctgt gggtgggctg gttcggcttc aacgccggca
    23341 gcgccctggc cgccaacggc gtggcggtga acgccctgtt gaccaccaac acgtccgccg
    23401 cgacggccgg cctggtctgg atgctactct cctggctgga cggccggccc agcaccctgg
    23461 ggatcgccac gggcatggtc gtcggcctcg cggccgtgac acccgcctcg ggctatgtca
    23521 cacccatggc ggccatgctc atcggggccg tcgccgcgcc gctgtcgtat tccaccatgc
    23581 ggttccggga acggcgccgg ctggacgaat cgctggacgt ctgggcctgc cacggcattt
    23641 ccagcacctg gggcatgctc gccacgggcc tgttcgccac gatcgccgtg aacggcgacg
    23701 gcgccaacgg ccttttcttc ggcaacccgg cccaggtcgg cacccagctc gtggccatcg
    23761 gcgtcacgat cattttttcc tttgccgcga cgtatgtcct cgccaggctg ctgcacgcca
    23821 gcatcggctt gcgcgtctcc gccgcggagg aagaggtcgg cctggacatc agctcccacg
    23881 gcgaacgctc ctattcgtga accgggcctg aacaaacggg tttcattgga gagggatcat
    23941 gctgaaaaag attgaagcca tcattcggga agacaaggta accgacgtga ccgacgccct
    24001 ggccgagatc ggcatcgtcg ggctgaacat gttcgaggtg cgcggccgcg gccgccaggg
    24061 gggcattcag ctggtggggc gcgccggcac gtacccggtc accctgctgc cgaagatcca
    24121 gatcaacatc gtcctgagcg accgcaacct ggaagacgcg atcgacacca tccggaaggc
    24181 ggcctttacc ggggagccgg gggacggcat cctcttcgtc acccccgtgg aggacgtcgt
    24241 gcgcgtccga accggagagc gggggcaggg ggcgatgatg tatccggggg acatcgactc
    24301 gaaaaaggcc aaataggacc cggcggtccg tggcgggtcc cccgcgggcg gtcggatttg
    24361 cggcgtgcgg cggctgtctc cggatcgcgg cccgcggcct cgcccggcgc ggttcatgtt
    24421 tgtactgagg ggtcgtctaa cggcaggacg acaggttctg gccctgtaaa ttgaggttcg
    24481 agtcctcgcc cctcagccat ttttcaaccc aaacgggctt ctccgccgtg ctctccatcc
    24541 acgctgcgag catgcccctc ccctgcgaaa cctggatttt tgaatgccgt gtcccgtgcc
    24601 ccggaacggc gcatcgggac ggaacggctc acagtcccag gtccgccgtc tcgaagcact
    24661 gcttgttcag ctccgccggg ggggtgagag ggtaggcgcc ggtgtagcag gcaaggcaga
    24721 agctgtttgg ggcaaggccc gtcgccgcga ccatgccgtc gagggtcagg taatggaggg
    24781 aatcgaggcc gatgaagtcg gcgatcgccg cttcctcgcc cttctgggcc gcgatcagct
    24841 cccccttgga ggagaagtcg atcccgtagg gacaggggaa gcgtgtcggc gggcagctca
    24901 cggccatgtg gatctccttc gccccgttct cccggaggtt ccgcacgcgg ttccggctcg
    24961 tcgtcccgcg gatgatggag tcctcgacga tcaggacccg cttgcccgcg agcagctccc
    25021 ggacggggtt cagcttcacg cgcacgccga aatcgcgcat cggctgggtg ggctggatga
    25081 acgtccgccc cacgtagtgg ttccggatca tccccatctc gaaaggcagg gcgctttcct
    25141 cggcgtatcc cagggcggcg tagttgcccg agtcgggaaa gggcatgacg aggtccgcgt
    25201 ccgggcggta ctcccgggcg aggcggtgcc cgaggcgctt ccgggtgagg tagacgttcg
    25261 tcccgaagac gtggctgtcc ggccgcgcga aatagatgag ctcgaagatg cagtgggcgg
    25321 gcttctccgg gccgaacggg gtcaggctgt ggagcccctc gccgtcgatg acgatgatct
    25381 cgcccggcgc cacctcgcgg acataatgcg cgccgacgag gtcgaaggcg caggtctccg
    25441 aggcgatgac ccagccgtgg ttgtcgagct tccccaggca cagaggccgg aacccccgcg
    25501 ggtcccgggc ggcgacgacg cggtccttcg tcagcatcac gacgctgtag gccccttcga
    25561 tctggtagag ggcccgggtg agcgcgtcct cgaaacgggt ccggaagaag ggcgccatca
    25621 ggtggatgat cacctcgctg tccatcgtgg attgaaagat cgacccccgc gcctcgagct
    25681 gcgaccggag ctcctgggca ttgatcaggt tgccgttgtg cgccagggcg tagtattcgt
    25741 tgccgtggtg aacgaggaag ggctgggcgt tcgagatgac ggacgaaccc gtcgtcgagt
    25801 agcggacgtg gccgatggcg agccgcccgg gcagttttgc gaggtggtcc tcccggaaga
    25861 cctcggagac gagccccatg cccttgcgct cccagatctg gcagccgtcc gaggtggcga
    25921 tgccggcgct ctcctgcccc cggtgctgga gggcgaagag cccaaagaag gccacgcggg
    25981 ccgcgtccgg atggccgtat acggcaaaga cgccgcattc ctcccgcgga cgcccgtcgg
    26041 cggtcccgca gcctcggaaa cctttcgcct ttttcaccgc ttcacccttt tgccttcggt
    26101 cccttgccgg acgaccgcgg catccctttt gccccttgtc ccccgcccgc cgcccgtttt
    26161 gtgataggcc tgcagcggcg ccacgtccca cggaccggcc cgcatcgccc ttaccgcctc
    26221 gcccagggcc cgggcgcccg cgatcgtcgt cgtgtagaga atgttgtaca aaagcgtgct
    26281 ccggcggatc tgcacggcgt cgcgcgtgga gagccggccg acgctcacgt tgatgacgat
    26341 ctggatgtcg ccgttcttga tcaggtccac gacgtggggc cggccctcgc tcagcttgca
    26401 gaccgtctcc acccggacgc cctggcggtt caggtgcgcc gccgtgccgc gcgtggcgga
    26461 aatccggaag cccagcgcct ggaactgtcg ggccaccggc acgatccggt ccttgtagag
    26521 gtcgtggacg ctgataaaaa cccgccccgc gacggggaag ggaaatcccg ccgccatctg
    26581 cgacttggcg aaggcgatcc cgaaggaccg gtcgatcccc atgacctcgc cggtggattt
    26641 catctccggc ccgagcagga tgtccacgtt ggggaagcgg ttgaacggga agacggcctc
    26701 cttcacggcg atgtgcgcgg gatggatgcg cctcgtgaac ttgagatcca ccagggattt
    26761 ccccagcatc accttcgttg ccagcttcgc caggggcacg ccgatcgcct tgctgacgaa
    26821 gggcacggtc cgggaggccc ggggattgac ctccaggacg tagagcacgc cgtccttgac
    26881 ggcgtactgg atgttcatga ggccgatgac gccgagttcg gcggcgatcc gtttcgtctg
    26941 cgtttcgatg gtcttgacga gcgccttgga gagggtcatg ggcggcagga cgcaggcgct
    27001 gtcccccgag tggatccccg ccgcctcgat gtgctccatg atcccggcga cgacggtggt
    27061 ggtcccgtcg gacagggcgt ccacgtccac ctcgatcgcg tcgtccagga acttatcgat
    27121 gaggatgggg tggccgggcg agacgtcgag ggcgcgcgcc atgaaggccg ccagggtctc
    27181 gtcgtcgtag acgatttcca tggaccgccc gcccaggacg tacgacggtc ggacgaggac
    27241 gggatagccg atccggcgtg cggccgcgag ggcctttgcg acgtccatgg cggtgtcgtt
    27301 ttccggctgg ttcaactcga ggcggcggac gatttcctgg aagcgctccc ggttctcggc
    27361 ccggtcgatg gcatcaggcg aggtccccag gatccgcgcc cccgccgcct ccagtccccg
    27421 ggcgaggttg agcggcgtct ggccgccgaa ctggacgatc agggggacgg cgccgacatc
    27481 gcccctccct tcggtcgcca ggatgtggag gacgtcctcc agggtcaccg gctcgaagaa
    27541 gagcttgtcc gaggtgtcgt agtccgtgct cacggtctcc gggttggagt tgaccatgat
    27601 gctctcgacc ccctcctccc ggagggcgaa ggaggcgtgg acgcagcagt agtcgaactc
    27661 gatcccctgc ccgatgcggt tcggaccccc gcccaggatc gcgaccttcg gccggtcgga
    27721 ggggcgggcc tcgttctccg tctcgtaggt ggagtagtag tagggggtgt aggcctcgaa
    27781 ctcggcggcg caggtgtcga cgagcttgaa gacggggccg accccctcgg ccaggcgcca
    27841 gtcgcggatc ccggcctcct cccgtcccca gagctccccc aggcggcggt ctgagaaccc
    27901 ccaggttttc gccgcgcgca gaagcgagac cgagggcgag gcggccttca gttccgcctc
    27961 ggcttcgacg atcgccccga tctgccggag gaaccagggg tcgatccccg tgaggcgccg
    28021 gatctcctcc acgtccatcc cggcgcccag ggcggcggcg atccagaaga gccgctggga
    28081 gttgggcgtc accaatttgg gtgcaaaaaa atccgccggg cgcgcccccg ccccctccgg
    28141 caggggcggc acgaggccga agcgcccgat ctccagcgaa cggaccgctt tctggagggc
    28201 ctccttgaag gtccggccga tcgccatcgt ttcgcccacg gacttcatcg aggtcgtgag
    28261 gacgtcgtcc gtctcgggga acttctcgaa ggtccagcgg gggatcttga cgacacagta
    28321 gtcgatggtg ggctcgaagg cggccatcgt ctcgcgggtg atgtcgttcg ggatctcgtc
    28381 cagggtgtag cccaccgcga gcttcgccgc gatcttggcg atggggaagc ccgtcgcctt
    28441 ggaggccagg gcggacgagc gggaaacccg ggggttcatc tcgatgacga tcatctcgcc
    28501 cgtgtccggg tgcacggcga actggcagtt cgagccgccc gtgtccacgc cgatctcgcg
    28561 catgatggcg aaggcggcgt cgcgcatccg ctggtactcg acgtcggtca gcgtctgggc
    28621 gggggcgacg gtgatggatt cgcccgtgtg gacgcccatc gcgtccatgt tctcgatggc
    28681 gcagacgatg acgcagttgt ccgcccggtc gcgcatcacc tcgagctcgt attccttcca
    28741 ccccagggcg gactcctcca gcatgacctc gtggatcatg ctcgcgtcga gccccccctt
    28801 ggcgatctcg gccagctctt cgcggttgta ggcgatgccg ctgccggtgc cgccgagcgt
    28861 gaaggacggg cggacgatga cggggaagcc gatctcggcc gccaccgcct gggcctcgtc
    28921 gagggtgcgg gcaaagccgc tccgggggac gcgcaggccg atgcggccca tggcatggcg
    28981 gaattcctcc cggtcctcgg ccttgcggat gaccgccacc gtcgcgccga tcatctcgac
    29041 cccatgggcg tcgagcacgc cggactcggc cagggcgacg gcggtgttca ggccggtctg
    29101 gcctccgagc gtggggagga gcgcgtccgg gcgttctttg gcaatgatct tcgccaccgc
    29161 ctcgggggtg atgggctcca cgtaggtccg gtccgccgtt tccggatcgg tcatgatcgt
    29221 cgccgggttc gagttgacga gcacgacctc gtacccctcc tccttcaggg ccttgcaggc
    29281 ctgggtgccc gagtagtcga actcgcacgc ctggctgatg atgatcgggc cggagccgat
    29341 gatcaggatc ttatggatgt ccgtccgttt cggcatgggt tattcccact cgatcgtgct
    29401 gggcggcttg gagctgatgt cgtagacgac ccggttcacg ccccgcacct cgttgatgat
    29461 ccgattggag acgcggctca acaggtcgtg gggcaggcgc gcccagtcgg ccgtcatcgc
    29521 gtcgtcgctc gtcacggccc ggagcgtgat gatgtgctcg taggtccgcc ggtcgcccat
    29581 gaccccgacg ctcttgatgg gcaggagcac cgcgaaggac tgccagagcg tcaggtaaag
    29641 ccccgccgcc cggatctcct cgtcgaagag ggcgtcggcc ttcttcagga tcgtgagccg
    29701 tcccgccgtc acctcgccca cgatccggac ggccagtccc gggccgggga agggctgtcg
    29761 ccagacgagg tcatccggga gcccgagttc cctgccgagg aggcggacct cgtccttgaa
    29821 caggtgcttg agcggttcga taaggcggaa gcgcatccgc ttcggcaggc cgcccacgtt
    29881 gtggtgcgac ttgatcacgg cgctgggacc tccgaaggcc gatcgggact cgatgacgtc
    29941 gggatagagg gtgccctggg cgagaaaggc ggcgcctttg atcttcttcg cctccgcctc
    30001 gaagacctcg atgaagatcc ggccgatgat cttgcgtttc ttctccggat cgacgacacc
    30061 cttcaaggcg gaaagaaaca gccggcccgc gcgggcgaag cggacgtcca tcgcgagatg
    30121 ttccgcaaag agcgcccgga ccttctgccc ctcgtcgagg cgcaggacgc cgttgtccac
    30181 gaagacgcag gtgagctgcc ggccgatggc ccgctggaga agcaccgcgg ccacggacga
    30241 atcgacgccg ccggagagcc cgaggatgac ccggccgtct ccgacctccg cgcggatttc
    30301 ctcgacggcg tcccggatga aggattgcat cgtccaggtc ttcaggcagc cgcagacggg
    30361 aaacaggaag ttctcgatca tccgcttccc ctcggtcgtg tgaacgacct cggggtggaa
    30421 ctggaggccg tagaggcggc ggcgggggtc ctccagggcc gccacgggcg tgttcgccgt
    30481 ccgggccgtg acgcggaagc ccttcggcag gcggccgatc gcgtcaccgt gactcatcca
    30541 ggagcgcgtc gtccccgtga ttccggcaag gatcccttcg gcgcgctcga cccgaagttc
    30601 cgcaaacccg tattcccgct tccgggcggc gacgacctcg ccgcccaagg cgtgaaccat
    30661 ggactgcatg ccgtagcaga tcccgaggac ggggatgccg agggcgaaga gccccgggtc
    30721 cgcctggggc gcgttcggct cgtagatgga ggacggcccg ccggagagga tgatcccctc
    30781 gggcttcagg ttccggatcg cggcgagcgg gatcgtcggg ggttcgatct ggcagtagac
    30841 gcggtgttcc cggacccgcc gggcgatgag ctggttgtac tgggacccga aatcgatgat
    30901 cagaatcaag cgcctatcct ccctccatca cccgaacgaa cgcttcgaac agctccgtcg
    30961 cgtcgtgggg tcccggggcg gcctccgggt ggtactggac gctgaaggcg cgcctggccg
    31021 tcagggcgat gccctccgag gtccggtcgt tcagattgtc ccgcgcccgc tcgaccccgg
    31081 ccggcaggga ttccgggatg accacgaagc cgtgattctg ggaggtgatc tcgatccgcc
    31141 ccgtccggcg gttcaggacc ggctggttga tcccgtggtg gccgaacttg agcttgccgg
    31201 tccggccgcc cagggcctgg cccaggatct ggtggcccag gcagatgccg aacaggggga
    31261 cgttcccgac gagctcacga accgtcgcga cgatgtcggg cagggcggcg gggtcccccg
    31321 gaccgttcga gagcaggacg ccgtcggggc ggcaggccag gatgtcgcgg gcggcggtcc
    31381 gggagggcac gacgaggacc tcgcacccca ggcgctccag gtgacgcagg atgttgtact
    31441 tcaccccgca atcgaggaca acaacccggc ggcgcgccgc ctccgcgcgg gcggcggccg
    31501 tcagcgggcc gtccagggga acggcccggc cgtcctgcca gcggtacgac cggttgcagg
    31561 tcaccgcctt gacgaggtcc cggccgacga ggcccgggta ggaccggaca cgctggagca
    31621 ggccgtccag gtcctccgtt tccgtcgtaa ggatcccccg catggccccc gccaggcgca
    31681 ggcgccgggt gagcgcccgt gtgtcgatcc cctccacgcc gagcttgtcg tgcgtctcga
    31741 gaaacgcctt cagggtctcg cggctgcgcc agttgctcgg cacggcctga tactcccggc
    31801 agatgaaccc ctccaggtgg acgccggccg actccatgtc ctccgggttg atccccgtgt
    31861 tgccgatcag cgggtacgtc atcgcgacga tctggccggc gtaggagggg tccgtgagga
    31921 tctcctggta gcccgtcatc cccgtgttga agacgacctc ccccaggacc tcgccccggc
    31981 cggcaaaggc cgagcctcgg aacaggcttc cgtcttccag gaccaggagg gccggccggt
    32041 ttttcgtcag cagcgacatg gcgtcccgca gggtgcgtgc attttccccg gccccctcta
    32101 caggatcggg aggtatcttt ccagctcgaa ctggctgacg tggatccgga accggtccca
    32161 ctccagcttc ttgttctcca cgaacttgtt gaagatgtgg tcgcccaggg tctcccggac
    32221 gagctcgctc ttctccacca ggccaatcgc ctggtgcagg ctgcccggca gggaggtgat
    32281 ccccgcggcc gcgcgtacct tctcgtccat ttcgtagatg tcctcctcaa ccggttccgg
    32341 cagggggtac ttcttctcca cgcccttgag acccgccgcc agcatgacgg cgaaggccag
    32401 gtaggggttg caggccgggt cgggcgagcg gaactccatg cgggtcgcgt tctccttgcc
    32461 cggtttgtac atggggacac ggatcatggc ggagcggttg cgccgcgccc aggagacgta
    32521 gaccggcgct tcatagccgg gaacgagccg cttgtacgag ttcacccact ggttgcagac
    32581 ggccgtgatc tccggcgcgt ggaccagaag ccccgcgatg tagtgcttgg ccatccggga
    32641 gaggttgtag gcgtcccgcc cgtcgtagaa ggcgttcttg ccgcccttga acagggactg
    32701 gtgcgtgtgc atgccgctcc cgttctggcc gaagatcggc ttcggcataa aggtggcgta
    32761 gacgttgttc tgacgggcga tctccttgac ggtcgtccgg taggtcatga ccgcatcggc
    32821 catcgtcagg gcgtcgctgt agcgcaggtc gatctcgtgc tggctggggg cgacctcgtg
    32881 gtgggagtat tccacgcgga tccccatctc ctggagggcg aagatcgtct gccgtctcag
    32941 gtccgtcgcc cggtcgatcg gcaggccgtc gaaatagccg ccccggtcga ggggttccgg
    33001 cagggtgtcg tcggcaaagt agaagaattc gagctcgggg ccgacgagga accggtaccc
    33061 cagatcgccc accttcttga ggatccgttt caggacgtag cggggatcgc cctcgtaggg
    33121 ggtgccgtcg gggttctgga tgtcgcagaa catccgggcc acgggccggt ccgtcggccg
    33181 ccacgggatc agctggaagg tcgtcggatc cggcatggcg atcatgtcgc tctcctcgat
    33241 ccggcagaag ccctggatgg acgaaccgtc gaagcccatg ccttcccgga ggccgtcctc
    33301 gagttcgtcg ggggtgatgc tgaagatctt ctgcatcccc aagacatcgg tgaaccagaa
    33361 ttggatgaaa ctgaccttgt ggtccttgac ggccttcaac acatcctttt gcgttttggt
    33421 ctccaacatg gcgtgtcccc tttctttgga tgcgggttaa agcttgaaca ggaggtccgt
    33481 gtaggtcggc accggccaca gatcccccgg aacgatcatc tccagggcgt cgatgtcgcc
    33541 acgcagggcc tgcatggccg ggatcacttt atcccggcag gcttccgact tcccgacgat
    33601 gtcatcgagc ttctgcgccc gggcggtcgc ggcttccaat ctttcgaggt tcttgtaggc
    33661 ggacgccagg agcgccccga gtttcttcaa gagatccgtc tggacggggg ccgcaactcc
    33721 cgccgctttg aagcttgcga tcgattcggc cagaaacgtg ccgtattcgg tcgccgcgga
    33781 caggaactgc tttttcacca tgtcgatggc ggccagggcc tcgatgttga tctgcttcgc
    33841 atagatctcg acgtagatct cgtagcggga ctcgagttcc ttgctcgaga ggaccccgta
    33901 tctttcaaag agccggatcg ccttgtccgt cacgaaggcc ttcagggcgt ccaccgcgtt
    33961 ggtgacgttc ggcaggccgc ggcgggcggc ctccttcgac cattcctcgg aatagttgtt
    34021 gccgttgaag atcacgcggt gatgcttctt gtagatctcc ttgatgatcg tcgcggcctc
    34081 ggcgttcttg tccttggcct tttccaggcg cgtggcgatc tcgtcgagaa cctcggcagc
    34141 aatcgtgttc agggcgacat tggtcccggc gatcgactgc gccgaaccca ccatccggaa
    34201 ctcgaacttg ttacccgtga aggcgaaggg cgaggtccgg ttgcggtccg tgttgtccct
    34261 ggggaattcc ggcaacgtgt cgacaccgac ccggaggaac tgcgcgccct tcgcggagac
    34321 cttctggccc cgggccagct tctccaggat gtccgtcagc tcgtcgccta tgaagacgga
    34381 gatgatcgcc ggcggcgcct cgttggcccc cagacggtgg tcgttgcccg catgggcaca
    34441 ggtcgcccgc aggatgtccg cgtgggtgtc cacggccttg atcagggcgc tcaggacgag
    34501 caggaactgg acgttttcgc ccggggtgtt ccccggctcc aggaggttga tgccgtcgtc
    34561 ggtggaaagc gaccagttgt tgtgcttgcc ggaaccgttg atacccgcgt agggcttctc
    34621 gtggagcagg cagaccaggt cgtgacgcag ggccaccttc cgcagggtct ccatgacgag
    34681 ctggttgtgg tcggttgcga tgttcgtcgt cgcgaagatc ggggccatct cgtactgggc
    34741 gggcgcgacc tcgttgtgct gggtcttggc ggtgatgccc atcttccaga gctcggcgtt
    34801 gagatcgctc atgaaggccg acacccggtc cttgatggct ccgaaatact ggtcttccaa
    34861 ctcctgaccc ttgggcgccg gcgcgccgaa gagcgtgcgt cccgtcagca tcagatcgat
    34921 ccgctcctgg aagaattgcc cgtccaccag gaagtactcc tgttccggcc cgaccgtggg
    34981 acagaccttc gtggaagagg tgttgccgag ggcccggagc acccgcaggg cctgcttcga
    35041 gagcgccttc atggaccgga gcaagggcgt tttcttgtcc aaggcttcgc cgtagtacga
    35101 gtagaaggcc gtgggaatcg tcagggtcac gtttccggcc atatcggtct tcaggatcgc
    35161 gggcgaggtg cagtcccagg ccgtgtaccc gcgcgcctcg aaggtgctgc gcaggccgcc
    35221 gctcgggaag gaggaagcgt ccggttcgcc ctggatgagc tgcctgccgg aaaattccat
    35281 gatgatcccg ccgtccgccg tgggggagat gaaggaatcg tgtttttccg ccgtgatccc
    35341 cgtcaggggc tggaaccagt gcgtgtagtg ggtcgccccc ttcccgaggg cccagtcctt
    35401 catcgcgttg gccaccacct cagccacgtc cggcccgagc ggcaggtcct gttcgatggt
    35461 ctccttgagc atccgataca ccggcttggg cagccgcttt ttcatcacct tgtcgttgaa
    35521 cacattcgat ccaaacactt ccgtgagttc catgaattcc tcctcgttac aagacatccg
    35581 gcggcttggc cggccgggat gcccctcttc gaaacgtgac tcgatggtct cgaggaccgc
    35641 tattccagcg gctcattttc agtcaatcgc cagacccccg tcgggcaggc gccaacgcag
    35701 aaaccgcagc cgatgcagcg ggcggcgtcc acttcatagg tgaacgtccc atccccgggg
    35761 tcccgccggg caacggcccc ttcggggcag atctgctcgc acagaccgca gtcacgacag
    35821 gccccgcatg aggcgcaggc ggcggcacag gcggtcaccg acgcgaagcc gttcgccccg
    35881 gcgtcgaagt aagcgtgctt aacccgctcc ggctcgatcg gcgggagttg atcgaacgtc
    35941 tcctcccggc cgcggatcag cgcatcgatg gcccgggccg ccgttcgccc tgccccgatg
    36001 gcctccgtca gcaggcccgg acgcaccgcg tcgccgacgg cgaagacctg gggatcgctc
    36061 gtccgcccgc gcgcgtcgac ggcgatgaat cctcgctccc gggcgacgcc ctcgggcagg
    36121 aaaccaaggt ccggctggtc gccgacggcg acgatgaccg tctccgccgg caggacctcc
    36181 cccgttgtca gttcaacagc gtctttcgta atgcttttcg tgaaccgggg ccagaggaac
    36241 ttcgccccgg cggcttccgc gtgctcgcgc tcctcgccga aggaagcggg cgcctgaatg
    36301 tcgatcaggg tgatcgactc ggcgccgagc cgtgcggcct cggcggcggc gtcgcagccc
    36361 acgtttccgg ccccgatcac cacgacggtc cgtccgaccc gggccttgcc cgccttgctt
    36421 cggcggagga aatcgagcgc cgcctccgcc cgctcccagc cggcaacgtc gatcatgcga
    36481 ggcttccggg cgccggcggc gaccacgatg aaatcgtatt ttttcttgag ctcgtcgaag
    36541 cccttgcgcg tcaccggctt ccggacatga tggatgagac ccgccacgcg ggcgagttcg
    36601 tgttcgagaa cctcgtcggg gatgcggctc gcgggaatgg tttccgtgat tttcccgccc
    36661 aggcgcgagt ccgtttcatg gatgaccggc tcgtgcccca tcaggcgcaa ctgccaggcg
    36721 acggagaggc ccgccggccc gccgccgatg acggcgatct tcttgcccgt cggcggggcc
    36781 ggctccgggg gcgcggcctc gaggctggcc cggccgagga gggtgacgtc caccgcgggc
    36841 aggtttaccg accggcgggt acagtgctgc atacagaggt tcggacatag atggccgcac
    36901 acggtcgcgg ggaagggcgt gtacttcagc gccaggtcca cggcctcgga cagttttccc
    36961 tcccggatca gggcccaacg tttctggacg ggaatccccg tggggcagtg cgcctggcag
    37021 gggggcaggt atttttcatt ctcccagacg gggacgaagc ggcgcagatc ccccttcgca
    37081 atcacgtcca ccgggctccg gtccaggtcg gtgaggtcgc cgatcaggcc gcccgccccc
    37141 agttgcgcct cccagacctc ccggagaaat tgcggcagcg agcggtaggg gccggcgtgt
    37201 ttttcttggg tccggcgagc cgccaggacc tgccactgtc cgcggtcggc ggtcagcgtc
    37261 ttgtagaggc ccggctgatc gatcgcggcg agaaaacctt tcatgcccgc gatcagccat
    37321 cgccagtcct catcgtccac cggcgccaga acggcgtcgt tttcgctgta acccgggcag
    37381 gaaccccgga agaagatctt tccgcccacc atcccgacgc aggggcgata tcccaggccg
    37441 gcgcgctccc gctccacgcc gtgaccgcag acgaccgcgt ggccgccggc catgaattcg
    37501 gcgaaggagt ccccgacgcc gcccaggacc cagagttccg gcggggcgaa gcgggggttg
    37561 tgcttggtca tcgtcatccc acgggcgccg atgttcccgg cgacgtagat ctgcccctgc
    37621 gccatggcgt tgcccgcgcc gttcgtggcg ttgccccgga cgatgatccg cgccccggcg
    37681 ttgagccaac ccacatcgtc ggacgccgga ccgacgcagt cgatcacggt gttggggaag
    37741 cccatcgcgc ccaggcgctg cccggacgtg cccatgacgc ggatccggac ggtttcgttc
    37801 cccgcgcgcc agaggcggcc gccgatgccg tgctgcccct gggcaataat ctccagcgac
    37861 cggtgcccgt cggacacggc cttctggatg cgctcttcca ggacgcgcga atccacccgg
    37921 aggccctttt ccatgccggg aatcacccag gtcggtttac tcgcgccgtt cacatcccat
    37981 ctccccgtca ggcgtcgggc ctgtcacccg gtgcctacac cacatacttg atcttcagcc
    38041 gttccgccgc atggccgtcg gctatgccga tcgcgtcgga catgccgatc ggcagcgacg
    38101 tggatcgccc caggggcgcc acgatctttt tcaattccgt gtcgaaaccc ttgaagacat
    38161 cgacgagccg ctcggccacc ttctccggat cgagccgccg gtagaggcgc gggtcctggg
    38221 acgtgatccc cttcggacac aggccgatgt tgcagatgtt gcagcggtcg cgttcggagc
    38281 cgagacagcc ggccgccgcc tgcatgaggt acttgcccgt ctggacgccg ctggccccca
    38341 gcatgatcag ggccgccgcg ttggcggcca ggttgccgtt cttgccgacg ccgccgccgg
    38401 cgaagatcgg gatctcgttc tgtttgccca ccttgaccag gttcaggtag gcgtcccgga
    38461 cattgctcgc gatcggatgg cccatgtggt ccatcgagac gttgtaggcc gcccccgtcc
    38521 cgccgtcctc gccgtcgatg gcgagccccc ccgcgtaggg attgcgggtc aggttgttca
    38581 gcaccgcgag cgtcgtcgag gtcgcggaga tcttcggata gacgggcacc cggaatcccc
    38641 aagccatggc catggactgg atcatcttgg cgaccgcctc ctcgatcgag tatttcgtct
    38701 ggtgcgtcgg cggactcggg aggctgacgc ccgccgggac gccccggatg gcggcgatga
    38761 gccggttgac cttgtaccac atcaaaagcc ccccgtcgcc gggtttggcc ccctggccgt
    38821 acttgatctc gatggcgcag gggtcctcct gcatctcggg cagggcatgg aggatctcgt
    38881 cccagccgaa gtagccgctc gcgatctgga ggatgacgta tttcagaaag cgcgatcgca
    38941 ggaggcgcgg ggggcagccg ccctcgccgg tgcagatccg gacgggcatc cccatctcct
    39001 cgttcaggta ggccacaccc atctgcaggc cctcccacat gttgggcgac agcgccccga
    39061 aggacatgct gccgatgacg agcgggtaga tctcgcgcac ggggggcacc cagccgttct
    39121 ccttcgtgac tctcaggatt tcctccggcg gcaggacccg gccgaggagg gtgcgcagct
    39181 cgaattcatg gcgacccgcg tcgagggccg gatcggtgag catggagatg cggatgaact
    39241 tgatcgcgtc gaggacgctc tgggggtcgt tgcgccggcc gccgcgccgc cggggttcgc
    39301 cgccctgctg gacgtggaag cggagcttgt ccgtctcgtc gccgtgggcg ggcgcgatgg
    39361 acccgttggg gcacacgagg gcgcacatcc cgcagcccac gcaggcatgc gcggcgtcgg
    39421 tccgttggcg gatgccgtgg tagaggccaa cggcctgggt cggcttatcc gcgagcccca
    39481 ccgccgcggt cacgacgcgc ttgcggtgga cgccgagttc gatcgcctgc accgggcaga
    39541 cggcggtgca gcgcccgcag agggtgcacg tttcccggtt ccaaaggacc cgccagggga
    39601 ggtcccggac gctcaaggtc gcggggtcaa ttcgtcccat tggctccatt gtctgacctc
    39661 ttgacggtcc ggtcccacgt agaccgtgtc gtacttcatc ggttgaaaat cccgcgattt
    39721 atctcgggcg gggatggcgg tgtcaagacc gcacatctcc gaggagaagg cgatcatccc
    39781 ggggttgccg cccacgatgc cgggacggag cttcttgccg tcctggacca tgaacaggcc
    39841 cttgtccggc aggcagccca tgacgcagtt cggtccgtcc acgatcagcg gtctgcagct
    39901 ctgcttgacc ctacggagcc actcgccgtt ggggtgttcg gccagctcgt cgtccttcag
    39961 gggggtgatg atgtgcttgt acatctcaag ccccagtccc aattggttgc gcgtgtagtg
    40021 gaggatgtgg gtgaagacct ccgagtcgga ctggtagccc tggtagccct cgtaccgccg
    40081 ggaagtgaga aattcccgga tcgggacgaa ggcggtgttc tcgccgttcg tcatcgtagc
    40141 gatcccctgg atgaaaaagg ggtggcaggc gtaaatgtcg atggcgtagt tcgtgttctg
    40201 acggccctgc gcgaggacca cgcgggcctg cagttcccgg cggttcaggc ccaggtattc
    40261 cgcgacggtc agggggtcgc cgacctcctt gatcatgatc acgtccggcc agaagctgaa
    40321 gatcagcatc gacccgtcca cctcccccat ccggcgcagc tctaggcggg tcatcatcag
    40381 gcggtgatgc agttcggcct gggagaggac ctcccactcc ggcgggtatt cgtagacccg
    40441 gatcaggtag ttgtcccgct tcggcgttcc cggcggcggc gccatgggcg ggcgtatgga
    40501 gagcttgtac ttcaccatga agtccatgtc catcatgtac cggtcgaggg ccttgatccc
    40561 ggcgttggag aagatccccg acaggatggg cccgccgcgg aactgctcga agtccccccc
    40621 cagatcggtc aggaagagcc ccacgccgga gccgtcgtgg ccttccttca tgacgtcgag
    40681 ggcgcggatc gccaccatcg gggagagggg ttcggcgctc gttatggcaa ataagcggca
    40741 catgggaaat tcctttgtct ttcacgcccg gcggttttca ccgacggcgt gacgtctgtg
    40801 acgggaaagc gataaccgtg ccagatggac gggagcggac ctctgggtcg ctccggcgga
    40861 gacagcgccc tcgcttcgcg gccccggccg gccgacgggc ggaaacggcg ggtggacaag
    40921 gaggccggct tgtcgataca taattgtgcc caccggtccg ggagcgacaa atttgtcagc
    40981 gcctccggag cgggtcttcc cgggggccgc ctccccgccc ttacgtctca ccggtcgaca
    41041 gggccggggg cggcgccgga cggaattggc gggtgtcgat cccgagcttg tggaccttgt
    41101 actggacaat ccgtttcgtc gtgccgagca gtttggccgc ccgcgtctgg ttgccgccgg
    41161 cttcccgcag ggccgccatg agcagggccc gttcgcgcgc cgtgatctcg accttgagct
    41221 ttccccgttc ccgccgctcc tcctcgctcc ggccgctctg gagggaaggg ggcagatggg
    41281 cggtctcgac ggtgtccccc gtgcacagga ccacggccct ctcgatgcag ttttccagtt
    41341 cccggacatt ccccggccat ccgtaggcca ggaggatctc catgacggcc ggggagatgc
    41401 gcttcaccag cttgcccagg gcacgaccgt acttttcgac gaagtggtcg gcgagcagga
    41461 tgatgtcgct cccccggtcc ttgagcaggg gcagatagag gggaaacacg ttcaggcggt
    41521 aatacagatc ggcccggaaa cggccgtcgg ccacgtcctg ctccaggttg cgattcgtcg
    41581 cggcgatgat ccggacgtcg acccggaccg tcctggaggc gcccaggggc tggaactgcc
    41641 gttcctggag aacgcgcagg agctttgcct gtgcggcggc ggagagctcc cccacctcgt
    41701 ccaggacgat cgtgcctccg tccgcctcct cgaaccggcc gcgccgcctc tgccaggcgc
    41761 cggtgaaggc gcccttctcg tggccgaaga gttcgctttc gaccagggtg tccggcatgg
    41821 cggcgcagtt gacgaggacc aaaggaccct tccggcgggg actgttccgg tggatcgccc
    41881 gaccgaccag ttccttgccc gtccccgtgg cgccgtggat gaggaccgtg gtgtcggatt
    41941 cggccacctg cgccatcagg ccgaagacct cctgcatgac gcccgaggtg ccgatgagct
    42001 cgtccgacgg gctgcacgac ttacccagca tgtgccgcag gcgccggttg tcctcctcta
    42061 gggcccgggt gtggacggcc ttggcgatca ggtccgccac ggcgaccagg acggcgagtt
    42121 cccgttcgag gttttcgacc tgccgggcga ccttgtccgc cgacaggacg ccgacgaccc
    42181 gctggtcgta gatgatgggc acgcagagga aggcgagttc ggcgcggtta aggaaacgcc
    42241 gggcgcctgt gcggtcgaga aacagggcct cctggccgag attggccacg gccatgggcc
    42301 gcccggtctg agccaccttg cccgtgatgc cctcgccggg ccggtaggtg acctccatcc
    42361 ccccgatatc gacgccgtgg gccacgtcca gccaggcctc gccggtctcg cggtcgatca
    42421 gggagatcat gccgcgctgc atccccagca ggcggctcat ttccttgagc acccccgcga
    42481 gctcggcgtg gagctcaccg gggcgcacga ggatccgggc gatggcatgg agggccgcca
    42541 gttcctcgta actgtgaatg ctgatatcct tcgtttcggc aagccccacc tgggtgggac
    42601 tccagacttc ccggctcatg cgtccatccc tgaaaaatgc ctccaggcgt tcgcccctgt
    42661 tccgtccctc ccactcccgg cggtcagggc agggattgcg aagctcactt cgggtcgttt
    42721 cctatcatga tgcgatacaa aattgcaaca gaggattgtt cccgttttac caccttttgt
    42781 cttgacattc gggttaaaaa tccattagta agagccacgc gcgccatgat ggacacacgc
    42841 gccgtgtttc gacaggctcc aacaaaacac tcaacccatc ccgccggacc cgacccttcc
    42901 cggcgggacg cccaagaccg tgacggcaac cgatgtcagc ggtcgccgtc accagaaact
    42961 ccgcatcgac tcatcgttcc gcgtccctgc acaggggcgc gatgccatag acgaacacgc
    43021 ttcaaccccg cgccccggtt tcgcgcccgt gaaggcttcc cggcgatcag gggcttccgg
    43081 acgctctttc ttaacccgtt tgcgatcttc accaagcgcg acccgggatc cgatcgccgc
    43141 atcacccgcc cgtgtggagc ggcacgggat cctcaggctc cacaacgacg ccatcaacct
    43201 aaaaaggagg gtatctatga gtgacgtcta cacggaaaag ccatggctca agaactacga
    43261 caagaatgtc ccccccacgc tgaagtacga agacaagacc ttcgcggaga tgttccggaa
    43321 ggcggtcgag gcgttcccgg acaaaacggc gctccactac atggggaaaa agctcacgtt
    43381 tcgagacctc gatctcctgt cgaaccagct ggcccagtac ctgctcaaaa tcggcctgaa
    43441 gccggacgac gtcgtgggca tgcacatgcc caacatcccg gcccattatc tcggcctgat
    43501 cgccgtgcag aaggcgggct gcgtctcgac cggcctgagc ccgctgctca ccccccatga
    43561 gatggaacac cagttgaccg actcccgggc gaaggtggtc atgacggtcg atgtcctgtt
    43621 cgagaaggtc gcggaagtcg ccggcaagac gccgttctcc accgttatcg tcgcggagat
    43681 cgccgacttt ctccccggcg tgaaacgggt gctggggaaa ctcctgaaga agatccccac
    43741 cgcgccgatc cggccgctac ccggcaagac cgtgctgaag ttcatggagg cgattcgggg
    43801 gatgccgacc gaccgggtgg ccgtcaagcg gaccatggac gatacgatct tcatgatgta
    43861 cacgggggga acgaccggtc cttccaaggg ggcgatcctg acccagcgcg gctacatgta
    43921 caaccgcctg cagacgtgcg cctggctcga catcggctcc aaggacgtcg cgctgtcggc
    43981 cttcccgctg ttccacatcg ccgggctcgc cctcggcggg ttcaccatga cgagcggcgc
    44041 cacccagatc tgcgtcccaa atccgcggga ctcccatttt ctcatcgggg ccatcaaggc
    44101 ctacaaaccg acgatgatcg tgaatgtccc gacggtgttc ttcgaacttc tgaaggtgcc
    44161 ggaattcaag gcgctggatc tgagcaatct aaacttctgc ctgagcgccg ccgcgccctt
    44221 cccggccgag tacatcgccg aactggaaaa gatcatcggc gcggggaact tcatcgaact
    44281 ctacggcatg acggagatgt cgcccgtcat gtgctgcaac ccccgctacg ggaagaagaa
    44341 ggcctcctcg atcggaatgc ccatgcccga cacggagttc aggctcatcg atccggaaac
    44401 gggccagccg gcgaagctcg gggagcctgg cgagatcgtc gcccgcggtc cccagctcat
    44461 gaaggagtac tacgagaagc ctgaggagac ggccaatgcc gtgcgggacg gctggatgta
    44521 cacgggcgac gtggccaaga tggacgagga cggctatttc tacctggtgg accgggtgaa
    44581 ggacatggtc atcgtgtccg ggttcaaggt cttcacgcgt gaactcgacg atctactcgc
    44641 gacccatcct gacgtcgacg tggcggccac gatcggcgtc cccgacccgg cccgcccggg
    44701 ttccgagcgc gtcgccatcg cgatcgtcct caaacccggc gtcgacaaga gcgaggccga
    44761 gaaggaaaag ctgacgaaat tcctgaagga gaacgtcgcc ccgtacaagg tgccgaaggt
    44821 gatccagttc atggacgccc tccccacgag cggcgtcggc aaggtcctca agcgcgaact
    44881 gcgaaagatg atgtaggcac gccagccctg tctgaagaac atccggcgcc cggggacaac
    44941 ctgcccgggc gccgcgactg ttccaggaaa tcccttgtct tgaaaatcct cttgatataa
    45001 ggtgcctcaa cttttaaatt caccggtcta cgacggagac gagcgacatg gacgtgaccc
    45061 ctcaacagat tgaaacctgc atcaatgcga ttcgctttct ggcggcggac gccatccaga
    45121 aggcgaaatc cggccacccc ggcatgcctc tgggggcggc tcccgcggcc tacgctctat
    45181 ggatgcgtca tctgaagcac aaccccgccg atcccgcctg gatggaccgt gaccggttcg
    45241 tcctgtccgc cggacacgcc tccatgctcc tgtacgccct gctccacctg accggctatg
    45301 ccctgtccct cgacgacctg aaaaatttcc ggcagtggga cagcctgacc ccgggccacc
    45361 ccgaacgggg ccgcgtgccg ggcgtcgagg tgacgaccgg ccccctgggc caggggatca
    45421 gccacgccgt ggggatggcg atcgccgagg cccacctggc cgccgtctac aaccggcccg
    45481 aacataccgt catcgatcat ttcacctacg tcatggcgag cgacggcgac ctcatggagg
    45541 gcgtcaccgc ggaggcctgc gcgctggccg gccacctggg cctgggaaag ctcatctgcc
    45601 tgtacgacga caaccggatt tcgctcgccg gctcgacggc cctgacgttc acggaaaaca
    45661 tcgccggccg ctttaccgcc tgcggctggc aggtgctgcg cgtggaggag ggcggcgact
    45721 ggacggccgt cgaccgcgcc ctcgccgagg ccaaggccga tctcgcgcac ccgaccttga
    45781 tcgccgtccg aacggtgatc ggctacggcg ccccctgcaa ggcgggcacc gcggactgcc
    45841 acggttcccc gttgggcgag gacgagctca aaggcgccca ggagcgcctg ggctggcccc
    45901 aggagccgcc ctttttcgtg ccggacgagg tccgggaaca attccgcggc gcgcttcgca
    45961 cgggccaggc caggcaggcg gattggcagg cccgctggga ccgataccgc gaagcgtacc
    46021 cggaagcggc ggcggaactg ctccgggccc aggcgggaga gcttcccgcg ggctgggagt
    46081 cggcgctgcc cgccttcgcc gatccgtcca aggaggtcgc gacccgcaag gcctccgagg
    46141 cggtgctcca ggccctggcc ccgaagctgc cggaactcct cggcggctcg gcggatctga
    46201 acccgtccgt aatcacctgg ctcaaggggt acggcgactt ccagcgcccg gaccttcccg
    46261 aggaaggggt tcagggccgg atgggcggcg tctggagcta cgcgggccgc aacatccact
    46321 tcggcgtgcg cgagcacgcg atggcggcga tcgccggggg gatcgcctgc cacggcggcc
    46381 tgatccccta cgcctcgacc ttcttcacct tcgccgacta catgcgcccg tcgatccgcc
    46441 tggccgcgct catggagctc ggcgtcatct acgtgttcac ccacgacagc atcggggtgg
    46501 gcgaggacgg cccgacgcac cagcccgtgg agcaattgat gagcctgcgc gccgtgccca
    46561 acctggcggt catccgcccc gccgacgccg ccgagacggt cgaggcctgg cggctggcgc
    46621 tcctcaaccg ccggggcccc accgccctca tcctgacccg ccagggtctg cccgtcctcg
    46681 accggaccga actcgcgccc gcgtccgggt tgcgccgggg cggctatgtc ctgtgggagg
    46741 cgtcggccgg tcagcccgac gtgatcctga tcgggacggg ctccgaggtg aagctcgccc
    46801 tcgcggcggg acggacgctc gcggcggagg gaatccgggc gcgggtcgtc tccctgccct
    46861 cctgggacct cttcgaccgc caacccgccg actggcggga cgccgtcctg ccgccgggcg
    46921 tcacggcgcg ggtcgccgtc gaggcgggaa tccgcctcgg ctgggagcac tacacggggc
    46981 ttgggggcgc catcgtcggg atggacacct tcggggccag cgccccgtcg cccgtcctct
    47041 tcgagaagtt cggcatcacg gcggaacggg tcgtcgccga ggcaaaacgg gttctggcct
    47101 gacccctccc ccggtcccga ccacgaccag cgcctctcgc ggcggagacc cgcggccgcc
    47161 ggatgcgccg ccaggcggtt tgacatcgag agggcgcttt gttatacgtc cttggggcgg
    47221 cttcgagcgt gaagccgggc aaagccggat gcaggcgggc ggctgaacga ggcgggtccc
    47281 gccgggaacc cccggacacc aggacgacca aggagggcca aaccatgaca cgggatgaat
    47341 acaaaaagat catttccctg gccatcgaac gggaaatcga ggcgtatgag ttttaccgtc
    47401 tcgtctccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccctggccat
    47521 cgaacgggaa atcgaggcgt atgagtttta ccgtctcgtc tccgaacgcg ccaaagacaa
    47581 caacctcaaa tccattttcg gcgacctggc caaagacgag caaggtcaca ggcggctact
    47641 ggagggcttc ctgacgaagg ccccggagaa gatgaaattt tcggaagcga aggattacaa
    47701 gatcgcggat gcggttccca cgccgcccct gacggcggat ctcaaaccga tcgaggggat
    47761 tgtcatttcg atcaagaacg aactcgaagc catgcagatg tatacccaac tggccaatgc
    47821 gagcaccgac gcggcgcaga agaatgtttt ccttcagctt gccgccatgg aacgcgggca
    47881 caagagccgg ctcgaggaca tctataccaa catggccttc cccgaggcct ggtagacgca
    47941 agagcggggc agccgggaat ggccggcctc cctatgtttg gcacctcacg ggcgacatgt
    48001 gcgcggggag cgatcccacg ggttcagcgg cacgtctctg accctacgtt ccagcaagat
    48061 agtctgcgcc ccccgcagtc cctccttcaa catccatcga attgtcaccg gttcgccgat
    48121 aacgggcatc acggtttgac tgaacgcatc ttttgagaag cgacccgcat cgcgttcccg
    48181 caagaagcga gacacgaatc gcaccgttca ggtcaaagcg tccgggaggg aaggcaaggc
    48241 gccctgcggc cttccacatc aggatccccg gaaatgaggg ggcaggttgt acgaacacag
    48301 tcggaggagg tcgtcatgaa cccatacaag atcgccgtga tcgtcggaag ccttcgcaaa
    48361 gattcactca accgcaagct ggcccatgcc atcatgaaat tggcgccgcc ggacttcacg
    48421 ttcaggcagg tcgagatcgg aaacctgccg ctttacaatc aggacgacga cgcgaatcag
    48481 gcggaatcgg tcaaacagat gaaacgcgaa atccgggagg cgcgggggct tctgttcgtg
    48541 acgcccgaat acaaccgttc gatccccggc gtcctcaaga acgcgattga ccaggcttcc
    48601 cgtccccatg gccaaaacga ctgggcggga aaaccggccg gtatcctggg cgtttcgatc
    48661 ggcgtcatcg gaacggccat ggcgcagcag cacctgcgga atgtcctcgc ttttctggat
    48721 gtacgaaccc tggctcaacc ggaagcgttc attcatgcca aagacggttt atttgacgcg
    48781 tccggcaaca ttggaacgga cagcaaagaa ttccttcagg actggatgga ccggtacgtc
    48841 gcatgggtga agaagcacgt ggactgatgc tgcagaaggg ccttcattgc cgctcctgct
    48901 gaggtcggtc gtcaatagtg gacatcgacc ctttcatgca gccggtcgtt tttcgcagaa
    48961 tcatcgttca aatgcggcgg gagatagaaa atcgagtctg gcctgctttc tttgccagtt
    49021 atagaaaatc tcaatgtact ccgctatgtc ccgtatagcc gcttgtcttg ttgcataccg
    49081 gcagtggtgg atctgttcat tcttgagcat gccccagaag ctttccatag gggcgttgtc
    49141 atagcaattc cctttccggc tcatgtacgc cctcatctat tgattaagat atcagctgcc
    49201 agaagcgcaa gcaggtcaac gttattgacc tcgacgccgg gagcatttaa cgatgctaaa
    49261 ttgatccacc aaagtggaca accggcgaca atcgctgcgt ctaaaaccaa agagcctgaa
    49321 g
//
LOCUS       DAOOLJ010000013        48992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_505_length_48992_cov_16.906553, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000013 DAOOLJ010000000
VERSION     DAOOLJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 48992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48992
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_505_length_48992_cov_16.906553"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLW16_04115"
     CDS             <1..531
                     /locus_tag="PLW16_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HPB70122.1"
                     /translation="WQVYNTEFSSPFRETNYEPEFLLTYPTNIDLWALQSRFIQVGIN
                     HQSNGRGEPLSRSWNRFTANFGFERGVFNMILKTWVRIPEKDSQDDNSDITSYLGYGE
                     LWAGTIWGNYHLQLMFRNNLRFDKNRSALQCEVSFPLLEYLNGYIQYFVGYGESLIDY
                     NHYTNRIGVGVMVKDW"
     gene            827..2506
                     /locus_tag="PLW16_04120"
     CDS             827..2506
                     /locus_tag="PLW16_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70123.1"
                     /translation="MADYPWFRNYDAGVPRTLAPYPDKTLLDVVAETAGQRPGHTMMW
                     FKGNVITYARFQTYVEDVARALAGLGVKRGDRVALLMPNIPQIIICQFAVWKAGGVAV
                     PVNPLYSESELVHALKDCGAEMAVVMTPFYGQLKNIQPKTRVKTVIATGVKEYLTPFK
                     RLMFGLLMEKKQGHYVELQAGDLWLQDIIRQYAGSPSPDFKLGFDETGLILFSGGTTG
                     VPKGVMLSHGCILMNGMQTRAWFGKLLVDWEDVTILLMPLFHIYGNIILVSTLLNARV
                     PIVLVPNPRDVPDLLATIRTTKPKFFPGIATLFIGLMNHPDVKSGKIDFRSMKMCVAA
                     AAPLLPETKKSWEALTGGKLVEAYGLTESGIIAMGPILGKWKEGAVGLPTPDVQVKIV
                     DIETGKKEMTVGETGEIIVRAPHLMQGYWNNPEATAEMLRDGWLYTGDVGHLDSDGYL
                     FITSRKKELIKPSGHQVFPVEVEEAIAKHPAVLEVGVAGVKDAAQGEAVKAWVVLQPD
                     QPCTAEELQAFCKERLTAYKVPKHIEFRDSLPKSLIGKVLRRVLQEEELKK"
     gene            2882..3082
                     /locus_tag="PLW16_04125"
     CDS             2882..3082
                     /locus_tag="PLW16_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB70124.1"
                     /translation="MSEGKVKWFNDKKGFGFIEEDGGKDVFVHHSAIQGDGFKSLSEG
                     DRVSFDVTQGAKGPAAENVRKL"
     gene            3538..3864
                     /locus_tag="PLW16_04130"
     CDS             3538..3864
                     /locus_tag="PLW16_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70125.1"
                     /translation="MGSKQKRVQIGQKESFGRKLKARLALLSERGIKSHEIDKDPLVK
                     NLRANIRAINVRMQAFEASEKKNEALAKMKADKAAPPQESPEGTKRKKVKEPPAEGKE
                     KKKKKE"
     gene            4032..4979
                     /locus_tag="PLW16_04135"
     CDS             4032..4979
                     /locus_tag="PLW16_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB70126.1"
                     /translation="MIVDSHVHLFYENSDPMSWFIGSARTGAATFAKSTGELGNVEEL
                     YQQLAPEIMDDNGDKLVPRMDDAGIERAIILPIDLRLLSDEPSAFDQTYPTIEQKNRT
                     YFKATQRHKGRIYSCAGVDPRRHGAVDLFRKGVEEWGMIGLKLHPTAGYYPHDPKCYP
                     MYEAAREMGVPVIIHSGSEPAPLKCAYSQPKYIDTVAADFPEVTFIVAHCGHGWHKEA
                     IDLASMKPNIYCDFSGWQVEYLTNPDYFFGPLRYALDFLGPWRVLFGTDGPAYTVFMS
                     NKDWVNAIREHKSPSGIKFSDEEVDLFLGGAAAKLYGWL"
     gene            5030..5506
                     /locus_tag="PLW16_04140"
     CDS             5030..5506
                     /locus_tag="PLW16_04140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB70127.1"
                     /translation="MPPREKCFIKPDDGRRITALAPVEGTANGRYLVNGFFEYVGLVA
                     DPFLLIIKDGRVVEVRGGGREAQKVRDIIAAADTHGNNIGELGVATNHIKIFDGFSGT
                     IIDKMILGTIHIAIGKNTTFEGGKVYSNIHHDAVSEGMTLKVDNTTIIRDGKFMLD"
     gene            5617..7566
                     /locus_tag="PLW16_04145"
     CDS             5617..7566
                     /locus_tag="PLW16_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB70128.1"
                     /translation="MICIQNLSLSFGDKVLFDHLTWTITERSRIGLVGDNGTGKTTLF
                     RTLLGEIDHYDGTVQLPKNRTIGYLPQDIVSLEPVTMFTYLKKQSGIDRLERDLAAQE
                     AAIAQADPKGAAYPALLRAYEETLARFQSRDGYAFEARARQICRGFGFGPDDFDKDCT
                     TFSGGWKMRILLAALLLARPDILLLDEPTNHLDTESMEWLESYLRDYPGTMIAIAHDR
                     YFLDKLAGQIVELAQGRITAYAGNFSAYLTEKERRLEALRKEMALQRAEIKRTEAFIE
                     RFRFKATKARQVQSRIRQLEKFSPVQAAAHEKTVRIQFPAGPQSGREVVTTRDLAHRY
                     GRNEVFRDVGFTVRRGDRLALVGVNGSGKSTLSRLLAGIEAPAAGEIRYGVHVRMAFF
                     SQDGSANLDPARTVWEEVQAVSSQDDDQRKRNLLGAFLFSGDDIYKPVRVLSGGEKSR
                     LALLKILLTETNLLILDEPTNHLDLKTKEIFQEALLGYAGTLVIVSHDRFFLDRLANR
                     VIELREGRAQEYNGNYSYFIEKRREAEDGAAVPGSAAVNGAPAPKDVRQRSKEERRLE
                     AEERNRLYRARSGLEHDLAATEERIARLEEEKAANEALLCLSETHRDARKVRDLSREL
                     QALAAELEDLYARWNDLTEKLAEIR"
     gene            complement(7563..8081)
                     /locus_tag="PLW16_04150"
     CDS             complement(7563..8081)
                     /locus_tag="PLW16_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB70129.1"
                     /translation="MKDRLNALEVALNNELREREFYLRHATRTKNPVGAAMFRQIAAE
                     ELEHYERLHALKETWEANRQWPETVPLKVNGTVVKDVLKGVLKQADGAPAGDADDREA
                     LKTAIAFEARGTEFYAGLRDRVTDPREKAFFDLLASIEHEHYTSLKDTEEYLTDPAGW
                     FRKTDRGGLDGA"
     gene            8317..8931
                     /locus_tag="PLW16_04155"
     CDS             8317..8931
                     /locus_tag="PLW16_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB70130.1"
                     /translation="MFKSFRQKVGFAKRRFIEPPYPKRADGQVYLHIGCGKKNHPGFI
                     NIDAQPFPHVHVVTSDIARLDAFADGSADLIYMSHVLEHFKRAELDGILGEMHRILKP
                     GGRLRLSVPDFDKLLEVYRLSENDVRSMHTQLMGSQDHPYNIHYTVFNRRDLEALLLA
                     AGFSCVRPWDPWDGSEPRVKDRSMKIMKAGNQAIKISLNVEAVK"
     gene            8967..9956
                     /locus_tag="PLW16_04160"
     CDS             8967..9956
                     /locus_tag="PLW16_04160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB70131.1"
                     /translation="MSRLLVLTNNAERASFRQRIGAYLAPMRDEGIEASVEILPRGMW
                     ARSRLFARAGAFDGVLVHKKGLNVVDAHLLRKHARRIIYNYDDAVMHSDRRPERRTRS
                     HFVPWRRMVRLSDAVIVGSEHLARLGRAYNRQVHVVPLGLRVGDYACDAAKPGDGKVR
                     LVWIGSASTLDYLRALKPVLEAIGARRGNVVLRQICESFLDLDSMPVEKLVWDSGKRG
                     EHLGTADIGLAPLPDNPFTRGKCSFKVLEYGAAGLPVVASPVGTNAEHIVEGVTGLLA
                     GSPAQWEAHLVRLIEDAALREKMARAGRLHAAGYDVSLIAPRLISVIAQAIER"
     gene            9996..11378
                     /locus_tag="PLW16_04165"
     CDS             9996..11378
                     /locus_tag="PLW16_04165"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPB70132.1"
                     /translation="MTTPSTPLLLRRVFPHRADETIRCTRLLRSVPGKREVFDARWND
                     RPVVVKIFFHPCQARRHLRREWRGLNAVMARELTSQKPLFYGRTDAGRWALVVDKIVD
                     SQPATDVFASLPEGPERVELLKKVCRELAAYHDRGVLQQDMHLGNFLLKGERVFLLDA
                     ARMKFRSRPISRAEGIAQAALLSVILTDVDAGDIRELCRSYLDARGCAPSPVDEARMA
                     SLRSRYRSVAVERGLKKTLRTSKRYQRVAGKTVRGVYEKAFFGNADLRPLFDRLETLT
                     GAGEDAGSGKARAASILTWHGKKLVVQRYRERGLIPAIRRTLTASHAKRGWLNGYRLI
                     MLGIGTPRPVCYVEFHRGCFVRESFLITECIEGKTLAETLQDRGLTAGRRETLLQHVW
                     RQVHRLHEHRIVHGDLKQGHILISAQGPLLTDLDRMTVCAWDCLFKAWRDRDLQRLNL
                     DGPGEETDRV"
     gene            complement(11476..11958)
                     /locus_tag="PLW16_04170"
     CDS             complement(11476..11958)
                     /locus_tag="PLW16_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPB70133.1"
                     /translation="MFDLMGHRIFSWLEKRELSYWRSRMRVGKGVDLKKGLRVTKPER
                     VTIGDHVSIGPDVILQAHAPIMIGAYTLISAGVAVVTANHGLDRRGLDMRPEVPAPVR
                     IGRDCWIGAGALVLPGVTIGDGTVVGAGSVVSRDLPPGMICIGVPAKPVKPRPAPPDA
                     "
     gene            complement(12124..13533)
                     /locus_tag="PLW16_04175"
     CDS             complement(12124..13533)
                     /locus_tag="PLW16_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB70134.1"
                     /translation="MRALSSKITLLLVILSVAIPSALPAAVERRTALIIGNSAYSSGP
                     LKNPVNDATDMAATLQRLGFEIILRKDVRQQEMEESIRDFGHRLKKGGVALFFYAGHA
                     IQINGRNYLIPIGARIEKETDAKYKAIDAEMVLDEMANAGNPMNIVILDACRDNPVRR
                     SLRSTGRGLAIISDAPQGTFITYSTSPGQTAADGEGRNSPYTAALLKAINEPGLPIEQ
                     VFKKVRVSLEGMKQTPWELSSLKGEFYFAEGEQKVPAGQREAPPSPSASDLQDTGAAR
                     PPSINAGESRKQMNLALAQQAFNERKFAEPPGDNAIEYSRKVRMVDPEHSVALDLEKR
                     ATTAYENEAEFASMKNNEKRALEIYQRLFSLYPEKKQYLEKLASLDSATFPDISGVWY
                     PTHVSGQAYFSSDGMAIYKGFLIITVSGKWSCKDPRKRIFSIVWNHGFTDVMTLSPDG
                     QKLEGINNIGDPVSFVRNK"
     gene            13779..15467
                     /locus_tag="PLW16_04180"
     CDS             13779..15467
                     /locus_tag="PLW16_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPB70135.1"
                     /translation="MDPISDTAPSDFIRDIIADDFKTNRHEGRVATRFPPEPNGYIHI
                     GHAKSVCLNFGIAAQYGGTCNLRMDDTDPAGESLEFVESIIQDIHWLGFDWQDRLFFA
                     SDYFEQLYAFAVELIRAGKAYVCSLDADEIREYRGSFTEPGRESPYRNRSVEENLDLF
                     ARMRAGEFADGEHVLRAKIDMASPFIVMRDPVIYRIKRAPHHRTGEAWVIYPMYDFAH
                     CLSDALERITHSICTLEFENNRPLYDWFVDQLISWDHPRQIEFARLNVSYTVLSKRRL
                     IELVENGHVSGWDDPRMPTVAGMRRRGYTPEAIRNFCAEIGVAKNDTVVDIALLEHCV
                     RQDLNDRAPRTMGVLKPLKVVIDNYPEDRTETFTCPNHPQNPVLGSREVPFSRVLYIE
                     RDDFMEDPPKKYKRLGPGREVRLRNAYVIRYASHNRDAVGGEVTEVHCAIDPETRNAP
                     PADGRKVEGVIHWLSAAHAAPAEVRLYDRLFSVPDPAAAGDDFVKFLNADSLEVLKGC
                     FVEAGLTEAAPGARFQFERTGYFCLDEKDSRPERLVFNRIVALRDSWSKGQGKA"
     assembly_gap    15686..15785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15786..16517)
                     /locus_tag="PLW16_04185"
     CDS             complement(<15786..16517)
                     /locus_tag="PLW16_04185"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPB70136.1"
                     /translation="MNGRLTVHTAHVEDGEWLRRLARTVFDRYTGGLHRWALDDDDGI
                     RRIHLGERAGVLAVRLAGRTGCVKLFYDNRPRVICLNRLGFPKARLAFENGLELARRD
                     IPAPAMLGWAVDGRTGLALLVTELIADAERVDLWIAAQGLTEDLIGQVAGFVRRMHDA
                     GVGHDDLSLRNLLVGRRDGGLSLWILDYEDTVFRKAMSRRRRLRDLHHLHERALALSG
                     VEDRLLFLRHYLGDAGDVARWAEDLR"
     gene            complement(16737..17636)
                     /locus_tag="PLW16_04190"
     CDS             complement(16737..17636)
                     /locus_tag="PLW16_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPB70137.1"
                     /translation="MSNGAGKIRIVTRPDMVVNADFQSLLAENDLLDYERIFRREGGT
                     VVKKIEERSVVRLDLVQDGRPRRIFLKRHRAVRPDLGAWIGHGFSARGLSPGMAEFEN
                     IRDFRKSGLPTVTPVAAGERRTGPFRFESFLVTEDFAPYVSLERLIRERPERFAGPAG
                     TPWKRRLLSAIGRLAAAMHAQGFNHRDFNATHILIGPGDETGAVFLALFDFQRVDRRT
                     WLRMKWRVKLLAELFFTLPEPLFDREDRRLLYQAFRGAARIRPVDRFLLLLVERKIAR
                     IARHMRKVNARKQADRESGRRIG"
     gene            complement(17633..18763)
                     /locus_tag="PLW16_04195"
     CDS             complement(17633..18763)
                     /locus_tag="PLW16_04195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB70138.1"
                     /translation="MKVGLVCRHFLSTKGGLERYTVTLAAELLRRGHDVHVFAHSGER
                     LPGLHFHPVPFFPLSSPGKNLSFAFMASRRIAPLRLDVVQSMERIWRQDIFRASDGIN
                     PVQMAQRYPNPWLRRFKAVTPRRRALTFLERRIFERRGARFIMTNSRLVRDQVLRHYR
                     VSEDRVRVIYNSVDTSRFNPNVRGRLGAPLRRRYGIGPEERLILFAGNEFKLKGLSVL
                     LEAVAGLGAPNIRIIVAGSDSADPYRSRARRLGLGEAVVFVGYQKALEGFYGAADLFA
                     LPTRYDPFANVCLEAMACGTPVVTTRMNGVSEVIETGRDGFVLRDVSVAELTASLRAY
                     LAVDDPRVLGARACQKAASFTMEAHMRDLLALYADVIEDKRS"
     gene            complement(18760..20223)
                     /locus_tag="PLW16_04200"
     CDS             complement(18760..20223)
                     /locus_tag="PLW16_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPB70139.1"
                     /translation="MSPDSHLYFQQAKALYYGLFDQLLAYYIYVSPYPITVSLFYSVC
                     GNWIVAAQGVNILFGTLTIAVLYGLLRRFFADGVAALGALVFAFLPTFAEVSRDALRD
                     PMFWFFSALGLYLFILHIEKRRPLLLLGCSLCLALAAWARFEAGLYIAATAGWIFFLN
                     RPARGRDLLVFGLPYAFLGSLFLGLSFYLDLSLVEILNPKRILNLPLNIIAQYDALRD
                     ELKILARPAYIIGIKSYFFDRIRSLLWIIALVAPVVLIVETLFYVFFVVLIGGAVSSL
                     RRRWADGRIRYLAVVSILALGALYLQTLYTWHGASRHLAVFILPSFVFIGAGIEAGHA
                     LLRWRFRYRPAAAYALVGTVVLLTLLPPVLWVNFDRDKLVYREIGRYIAKREANTRPV
                     NVCGAFKRVVDVQFYANLNTPSATFSDYRALFHEANALSADSICRAPCDYLVRDEKGW
                     RESKVDFSHPSASCTFRELERWPSRKQGALILYEVSR"
     gene            complement(20354..21088)
                     /locus_tag="PLW16_04205"
     CDS             complement(20354..21088)
                     /locus_tag="PLW16_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPB70140.1"
                     /translation="MPNRFSTYLKKKVLKKDQNLVSLDDPYEVVRALLRGHAVRGILD
                     AGASNGHASKRILKNFPSAHAYAFEPNPMYREGLIDYARRDPRFHPQFAALSDHEGTA
                     ELNITASAGNASLLTPSGRLRDIDPDGAGIRKVVQVPLVTIDGWVERNGDVPIEFMKF
                     DIQGYELTALKGATRTLRKSTLIVYSEIWFNGAYEKCVLFGELDLFLREQGFSVYDIY
                     KPGYNRTGGQVIWANAIFIHADKVRP"
     gene            complement(21277..22431)
                     /locus_tag="PLW16_04210"
     CDS             complement(21277..22431)
                     /locus_tag="PLW16_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB70141.1"
                     /translation="MKSGHRGVFYEFILFGFYLASKLMRHKAHLLGRENLARIPTPIM
                     FAVTHDSYFEVPSLSRVYYALKPRPDFMVMAKNDFLSGRYLSTNYGKRSPLLRSILLM
                     IDKSGLPMAVFRKLKLAAIDRPFVEVLSKKKEELRQEISDQLSRFREGSAQGVSTLIF
                     PEGTTWGFGGLKKIRSSVYQVLENTFQETGRKVYILPINVKVDRLVRGAKDVFIRVGP
                     PIFFRKPKDEFNRTLYEILQGLHTITFSQVAAYYLKRLAEMKRETARTVLFRKELFLQ
                     NLEGILMEMNRLAQDRALPDFDPALRDRRYLSDKAERFLKYCLKKRYLHKKRLSDRKE
                     ALLLDIERILSSPPVKEFRKANPLAFCANELNSLGEQTIRPVFDARLRCR"
     gene            22687..23469
                     /locus_tag="PLW16_04215"
     CDS             22687..23469
                     /locus_tag="PLW16_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPB70142.1"
                     /translation="MAPVLRSRTTHLPILLLLGLLFLIPAAPGRGEFPKPVGTVNDFA
                     QVIGPEESRRIDALSREVLEKTGASVVVATFATLGEAEINDTVNGLYQAWGIGKQGED
                     RGVLIFLALKERKIRIETGYGVEGILPDGRVGEILDRHVVPFLRAGDYGKGLLNAALA
                     VSEVIAGDAGVTLTGSPAPTPPPVTRQRKGIDALTLLFLFLILIPLLGTRQGRAMLPW
                     ILLLLMSGSGGRGGGGFGGFGGGGYGGFGGGGSGGGGAGRSF"
     gene            23484..24215
                     /locus_tag="PLW16_04220"
     CDS             23484..24215
                     /locus_tag="PLW16_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70143.1"
                     /translation="MARRPKHPREIFAEITADYRRVFGDGLISLILYGSAAGPDYVPS
                     RSDLNFLVVLDGAAMDGLERAFGLVERWKKRGVATPLFMTKTDIATSLDVYPVEFLTM
                     REHHVRVDGEDVLEGLTFDREHLRLQLERELKGKILLLREHYLETEGRVKAVRELIRA
                     SVTAMISLFHGLLFLKALPIPAGRREIVRDAAEHFAFPADVFLTALDIRAGTDRLLDR
                     DVQAFFRGYLGAIQALCTQIDQWRT"
     gene            24296..24871
                     /locus_tag="PLW16_04225"
     CDS             24296..24871
                     /locus_tag="PLW16_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB70144.1"
                     /translation="MSTGKKALIVVLIVLLVLGISLYSFFKGNYNTFVRLDESVKSSW
                     AQVENQLQRRYDLIPNLVETVKGYAKQERDVLVEVTNARARVGGAATVPDKIEANNAL
                     SGALSRLLVVVERYPDLKSNQNFLHLQDELAGTENRIAVERKRYNDAVRTYNVAIRTF
                     PANLLAGLFGFTKAAFFEAPAAAQAAPQVKF"
     gene            complement(24861..25472)
                     /locus_tag="PLW16_04230"
     CDS             complement(24861..25472)
                     /locus_tag="PLW16_04230"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPB70145.1"
                     /translation="MGLLEKRTGEKVHSRTLDILTYPAGDRGILVTGELVEKRLRSYY
                     GVSGEIRSPHTVHHMIIRMLIEGLNLAVKEIEVEMPGVPREDCLETAQCLNRLVGMNI
                     VAGFTLKAREAVGGRRGCTHLLALLTAMAPAAVQGFWVHHAAKPAESDTSSRLMEQYL
                     IDSCWVWRREGPMAQELLQALADASPADRASARSADPSHPPKT"
     gene            complement(25625..26512)
                     /locus_tag="PLW16_04235"
     CDS             complement(25625..26512)
                     /locus_tag="PLW16_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70146.1"
                     /translation="MGAIPELRPAATVILVRDREGGPYEIFLMRRHRNQAFMGGAYVF
                     PGGRLDEGDGHPDLRAHLHGLAPEALAERLQEPALPPETALGLHLTAIREMFEEAGVL
                     LAVDASGAAVDFHAPETARRFAAYREVLHRGEMTLKDLAAREGLRFALDLLLPYAHWM
                     TPEIESKRFDTRFFLARLPAGQRPVHDEAELTASVWMTPAQALAEHEAGRIVLMPPTL
                     KTVEELAVHRTTETLFKAVSGREIPPILPQPFTPGGEFGVKLPFDPEYTIETFKLPPR
                     PGETCRIVTCKGIWRTATA"
     gene            complement(26602..27390)
                     /locus_tag="PLW16_04240"
     CDS             complement(26602..27390)
                     /locus_tag="PLW16_04240"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB70147.1"
                     /translation="MKLSEMYNLKGKTALVTGGGRGIGKFIATGFAEAGADVIITSRK
                     MKNLEATAQELAQAYGVQAVPIACDLAKDEEIQHMLQAVREKFPRIDILANNAGVTWG
                     APTLDYPLERWDQIFKVNVRAVWIITQQVARLMKEQGGGTIINVSSIMGSRGSEEMAH
                     PAVAYNSSKAAIDLLTMNLAVKLAPYKIRVNAIAPGFFRTDMMAYIEKPEFSAAYEMV
                     LNAIPLHRIGDMDDMKGLAVFLASDASAYLTGQIIHADGGMTAK"
     gene            27649..28671
                     /locus_tag="PLW16_04245"
     CDS             27649..28671
                     /locus_tag="PLW16_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70148.1"
                     /translation="MSASLDTARARANTARYRPGQRAGHYESFFQRANHPDRPLAFWI
                     RYTLFSPEDRPDAAMGELWAVFFDGERNRHWAVKREVPMGDCRFDADAFSVRVGEAVL
                     GPGHLKGAAASGGHHLSWQLTFDGAQPPLFLLPLGLYNTGFPKAKSLVGLPLARYRGF
                     IAADDRRIEIDDWIGSQNHNWGRRHTDHYAWGQVAGFETHPESFLEVATARIRIGRGW
                     SPFLTTLVLRHRGEEIRMNSLPRMLRARGSFCYFLWSFRSEDRRHRVEGTISAPREAF
                     VGLNYLNPPGGDKHCLNTKIAACEIRVTDKRAGGTAAPDVLFTRHRAAFEILTDERDH
                     GVAIQA"
     gene            28690..29874
                     /locus_tag="PLW16_04250"
     CDS             28690..29874
                     /locus_tag="PLW16_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70149.1"
                     /translation="MREKVLRTDGPTGSGRVVAYPLGDGRRLFLIRTYCPDRIGPGPT
                     NLYLIEDEALILVDTGLPTDMAKQFFYFWRRQSIPRGIEALPDDFSERELTSALALTG
                     HRIEEIDAIVLTHGHMDHYLLGRKIVARSNARVAAHVSDTAMICNPWGMARFMAERWP
                     VLMAMGMPPPKRHQTSESRRQTLRIDLSLHVDEPIAADGPLTVGDAVFPRLTVRHFAG
                     HSPGGICLLVDSENGSGRRMLCGDTLLYPITPHPDDLVAYLRTLKAMKTLEDISLTLP
                     AHGKAIRRLPERLAAIEQHHERRLRLTYEACRRPRSIWQIATMTGYFDVLVEPTQFNP
                     LAGQEAWVHAELLYLVGGLRRTHIREGVHYFENSGEAFAAIYERVRELIDDEQSTLLT
                     RR"
     gene            29884..31461
                     /locus_tag="PLW16_04255"
     CDS             29884..31461
                     /locus_tag="PLW16_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70150.1"
                     /translation="MLLIHPPVVKPAEPPAGIARLAGALRSNGVACHLWDANLEGFLS
                     LMAAAETPGKPAESDDAWTRRARRHAEAHLSSLRQWPLYRQPALYQRTVRDVNRLLHA
                     ASPTAIRISLSDYRDADLSPLRSRDLARAAAEPERNPFFPFFSDRLTRLVETRNPGTV
                     GFSLNYLSQALCTFAMIGHLRRRHPELEIVVGGSLVTSWATHLGNGSPLRALADDWIA
                     GPGEGPLLARHGIPAGDGADWPPDYDDLTPAAYLSPGTVLPFSASRGCYWRRCSFCPE
                     QAEKAPYRPLTAAQVRTQVADLRDRTQPALLHFLDNALSPALLGALADYPPKVPWYGF
                     TRFTPELARTEFCRALRRSGCVMLQLGLESGDQGVLDSLDKGIDLATVSRCLTALRTA
                     GIAAYVYLLFGVPTETPDAARRTLEFTVRHADRLGFLNLAIFNLPVQGPEAAPLETAP
                     FYEGDLSLYRRFSHPRDWNRGNVRRFLDREFRRHPALTPILQRDPPFFTSNHAPFFVM
                     TPFPSPSPNPFGKEPEP"
     gene            31458..32861
                     /gene="pyk"
                     /locus_tag="PLW16_04260"
     CDS             31458..32861
                     /gene="pyk"
                     /locus_tag="PLW16_04260"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB70151.1"
                     /translation="MKKTKIICTIGPACDSAKMIEKLMLHGMDCARLNFSHGLAEEHV
                     EKIRLLREVSERLGKPVPILQDLAGPKIRIGLLPESGVRLVPGQTFILTSREIPGSDA
                     AVSLSYPDLPREVRKGDRLLLADGLMELVVEDTDGTDIRCCVITGGVLTSHKGINLPT
                     GTIRAPALTAKDREDLAVGLAHGVDYVALSFVRTADDIRAVQELIRNRGLTTPVIAKI
                     EKHEALEVIEEILDVADGIMVARGDLGVEIPLAEVPLIQKKLIHLANVRGKTVITATQ
                     MLRSMIDSPRPTRAEATDVANAVLDGTDAVMLSEETASGDYPVEAVEFMLRLIERAET
                     GYPYADFLQAKPDPGVSAAVAKASCVLADHLGARCIIAHSQSGLTARALARFRPRQPI
                     IALSPSGETVRRLALVWGCQPIQVRDFKDTDDLIEKAAQAALAAGELAPGELAVITLG
                     HPLWTTGSTNMIRVKEL"
     gene            complement(32876..34390)
                     /locus_tag="PLW16_04265"
     CDS             complement(32876..34390)
                     /locus_tag="PLW16_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HPB70152.1"
                     /translation="MQKHEKPEIKRLRLGFETARDVLRDTLRNYQINGDTNQAAAIAL
                     YAILSIIPLFILTILMASLVFSSNPQIQQQLVQGAQGVSPYLSQSLLEQLGRIDQKRQ
                     ILGWVGIISLVWFSAMIFNAIETVMNIIFRSHVRRSYVLSKLLAVALILLGWIIIGAS
                     VGLAYVGSLLARQPLLMEHFAYLSLLHGVLFRYILPYAITVAFFTVVYKATPTARVSW
                     GAALMASLLFSALMEVAKHFFAWYIANYTRYHVIFGGLEAVVILVIWVFYVALILLFC
                     AELIASYQRRDILLVEKALLTPGKARSSVDERLLRRFGRVYPKGSFVFREGDTGSEMY
                     YILQGRILVQKQAGQIKKVLTDVGPGEYFGEMSTLIDAPRTASAEAASDSCLAVIDSE
                     TFHRMLRESDDVSLLMLRASSRRIKHTNEVLEELTEAWIELMAILYFLKEWPLAQGQD
                     PVTELVRYTGKDPGDIREVLRKLGREGILRFEGDRVTEFVRSEAWKLLNHQVFG"
     gene            34573..36216
                     /locus_tag="PLW16_04270"
     CDS             34573..36216
                     /locus_tag="PLW16_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HPB70153.1"
                     /translation="MRIRLPILPLCLATLLVCSAACGQRPSEPGPAGPGAESPPAHGD
                     VLVEGAIGDASNLIPLLASDSASHDIAGLVYNGLVKYDKDINIVGDLAESWEIGREGL
                     EITFHLRKGVRWHDGRPFTAEDVLYTYQVTIDPKTPTAYAGDFLRVKKAEVLDPYTFR
                     VRYDRPFAPALMSWGSAVLPKHLLAGRDITQSPLARHPVGTGPYRFKEWVTGQKIVLA
                     ANPDYFEGRPYIDGYVLRIIPDTATMFLELRAGGIDRMGLSPLQYTRQTETPLFRRDF
                     NKYRYLSFSYTYLGYNLKNPLFADPRVRRAIAHAVNKDEIIRGVLLGLGQPATGPLKP
                     WTWAYNPRVERYPYDPGKARALLAEAGWRDRNGDGVLDRDGRPFRFEIITNQGNEVRQ
                     KTAEILQRRLGEVGIEVKIRVLEWAAFVNDFINKRKFEATILGWTIPMDPDLYDVWHS
                     SKTGPEELNFISYRNPEVDALLEQGRSTFDLKLRKRCYDRIQEVLADEQPYLFLYCPD
                     ALPIIQARFRGIRPAPLGIGYNFIRWYVPHGEQRLVMTP"
     gene            complement(36231..37631)
                     /gene="thrC"
                     /locus_tag="PLW16_04275"
     CDS             complement(36231..37631)
                     /gene="thrC"
                     /locus_tag="PLW16_04275"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB70154.1"
                     /translation="MSSATIRYYSTNRELDGVPGITPFKGQVAFREALLMGQAPDEGL
                     FMPAAIPTLSLAEILALKGRPYPEAAFFVAEAFLKDEIARDDLKRLVEDAYPFSVPLE
                     RVTGRKYLLRLDQGPTASFKDFAARLMARLMGHFREAGRRLNVLVATSGDTGSAVGEA
                     FKGVPGIDVTILYPRAEVSGRQKRQLDTIGANIQALSIDGKFDDCQTLVKQAFADPDL
                     AAFNLTSANSINFGRILPQIVYYIWSYAQLAENGEAVVVSVPSGNFGDALGCEYARRM
                     GLPVATLVMPTNENDEFPNFLATGAYEKVQPSRACLSNAMNVGHPSNLARFFDLYGGT
                     VDRNGRVHRLPDVAEMRRRIFSVSVSDDETRRTIRRVYDQYGILLEPHGAVGWRGLEL
                     FLEVRGEENSPLCLVLETAHPAKFPEEIRALLGIDPVPPAGMRDLDQRTGEALSLRAD
                     YRAFRQYLLDRLKREA"
     gene            38000..39055
                     /gene="selD"
                     /locus_tag="PLW16_04280"
     CDS             38000..39055
                     /gene="selD"
                     /locus_tag="PLW16_04280"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:38054..38056,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPB70155.1"
                     /translation="MSDPEKAVRLTETVHGAGUACKIGPGDLHEVLKDLPFIADPNLI
                     VGMEHGEDAGVYRLRNDLAIIQTVDFFTPIVDDPYLFGQIAATNALSDVYAMGGRPLT
                     ALNIVCFPIHKLDTAILKAILRGGLDKLREADVLLVGGHSVEDNETKYGLAVTGVVHP
                     EKVLLNRGARLGDRLILTKPLGTGIVGTAIKGGVAAPELVDHALRTMTALNRRAAELL
                     SDATEVHAVTDVTGFGLLGHALEMVEDSNVGLVIDAARVPVFPGLRDLVESGIVPAGL
                     HRNRQYRERQIEAAPSCPAWLLDVLFDPQTSGGLLVALPEKDASAYLENLHANGLRQA
                     AVVGRVEASPPGRVLIR"
     gene            39220..40260
                     /locus_tag="PLW16_04285"
     CDS             39220..40260
                     /locus_tag="PLW16_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPB70156.1"
                     /translation="MKRAESILSKEITRNNLSRLTSLARKNDSLAILMYGAPDPDAVA
                     SAMALKEILGKTVGLAKCTFVSTDPVARQQNVELIREMKISIELLDKVQLADYRLIAL
                     VDAQPPFFGKALENITPQIVLDHHPRDGEWRADLEDIRPRYGALSTILMEYLLAAQIK
                     ISRRLYTALLYGIKTDTNSFERDAILEDVSAYYVTFSRANRQLIRRIELNQIPDRFIK
                     YFDHAYHYRRRYRDRIICYLGRVENTDVCVQVADFFLNLINIYYVVIAGMTKDRLIIV
                     FRGDGYRQDCGAIAQKAFGAYGSAGGHKSAARVEIALDPLRDLLKNDLTQEGVDHFLV
                     QHLRGKRKKKEP"
     gene            40257..41300
                     /locus_tag="PLW16_04290"
     CDS             40257..41300
                     /locus_tag="PLW16_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70157.1"
                     /translation="MNDFGLSKETSVGTYLDPMNGFRQKASVFEIRRDEIGGVTSRIL
                     PYRFKMPERPDLSGYLEKSPPAICPFCPDLIDPLTPRFTADICPEGRFRRGRATLFPN
                     AFPYDRHNAVITLSDEHFVGLDAFRPESMQDGFHLAWDYFERMVKIEPTLRFASLNWN
                     YMPPAGGGLIHPHLQTILGEKPTRFAARLLDGAGAYRERMGRDLWTDFLAREKTLGER
                     HIAQTGPVTWLASFAPKGMGGEVAFYFGERTALRQISAADLLGFLDGLACVLGYLHAN
                     NFISFNLALYGSWEENRDFTVQGRISPRFSIPPLGTSDINYIEKYHDEVICPVVPEDL
                     CREILPFFAAVIP"
     gene            complement(41302..41580)
                     /locus_tag="PLW16_04295"
     CDS             complement(41302..41580)
                     /locus_tag="PLW16_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB70158.1"
                     /translation="MPIYEYKCKKCGKEFEVFQHISDPAVKSCRFCKGPVNKLMSLSS
                     FHLKGSGWYVTDYGGKKVPHTEKKEASESAAPAETPKAEAAPKGGSEE"
     gene            complement(41600..42124)
                     /locus_tag="PLW16_04300"
     CDS             complement(41600..42124)
                     /locus_tag="PLW16_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70159.1"
                     /translation="MYFSPEQISLADQAFVRAEALASGYFRLGPGALKAHRYDVRTKA
                     YLKEHEVQGQAFAHLCKYEYGKETGDVGAGDRHFYRICLQDDRILDAVKRAHSFIRLS
                     PLLLYIATHELVHVIRFDEGVIPFDAPREVKDEEEETVHQITRRMLQPAANPELGLVL
                     DCFSDRYRIGDLDH"
     gene            complement(42239..42688)
                     /locus_tag="PLW16_04305"
     CDS             complement(42239..42688)
                     /locus_tag="PLW16_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPB70160.1"
                     /translation="MDDRVREAIFRRVAEEPFAQKFGLKLLALDDGHSRVEMRFTPDM
                     ENLFGMAHGGALFALVDEAFETASNSHGTMAVALSMNVAYLASPRPGALLTAEAKELN
                     RSNRTAVYEIRVEDEDGRLLVSCQALVYRMAKPLPFLTDTAPPRPEP"
     gene            complement(42709..43611)
                     /locus_tag="PLW16_04310"
     CDS             complement(42709..43611)
                     /locus_tag="PLW16_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB70161.1"
                     /translation="MSKRQPAGLCVWACFLSLFLAMTFGSPAEAAKRAAPSRGPQLAE
                     RYSLIQSAEVSGDRGKNVPETERLNRAVGEARKAALKRAQQYLERKFRGVGFGYEILR
                     TAPEGSVRILDRRTLGPDETGRPRVRIDMEVDYTFRPASGSDATSRQPAIDRAGLLNV
                     RLWTDKKRYRAGEPVTVYFEGNRDFYAKLVRVTASGAIEQILPNNYRQLSSFRKGVRY
                     QAPGEGDRFVLSPPPGSRETFLIVASPYPMSHINMKTLAGGIYQYRSRWSSFERAVRS
                     GLTLGAEDVAEFFTAGCEIEVSLP"
     gene            complement(43683..45170)
                     /locus_tag="PLW16_04315"
     CDS             complement(43683..45170)
                     /locus_tag="PLW16_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPB70162.1"
                     /translation="MVNISSEAKVGLFVLVGLIILGYMSFRVGQQGFGLKNGYTVRAV
                     FPSVAGLDRDASVQIAGVEIGRVEEIQLQDGQALVTLRIRPNVKLGNDAAATIKTHGV
                     LGDKYIELAPGTNTQAFLKEGDQIQQVESQADIDRLLHQIALIADDIRGVTSSLNKAM
                     GGERGEASLEEIVENARLLTKNLNALVVNNEEALRFTLDNARQLTDNLNRVVVTNDAK
                     ITDVMDNLKDASKQMEQTFAQLNEITGSINRGEGSLGHLVKNQETAQKLSQTMTSLQE
                     VSDKINQGKGTIGKLINDEQTARNLDETIKGINRYVSKSEQFRTYLNYRGEYLFDKSD
                     GKSYVDIRIQPKEDRYYLVGVVADPRGRRTVKERITNGVMTRTEEWEKDGLLFNAQVA
                     KRFRNIVLRGGVLESTGGVGLDYLMLNDRLKLSFEAFDFNSDDDDRAHLKAGMEFQVF
                     KHIYLTAGWDDFISKRSNSSPFAGFAIRFEDDDLKYLLSSVPIPK"
     gene            complement(45185..45931)
                     /locus_tag="PLW16_04320"
     CDS             complement(45185..45931)
                     /locus_tag="PLW16_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70163.1"
                     /translation="MIRLEEVHKAFGRQTVLDGVDLEIESGKTTVIIGRSGGGKSVLL
                     KHIIGLMKPDRGRIFIDDVDITRLNDRDLNEIRKKFGMLFQEAALFDSMTVGENVAFP
                     LREHTPYPEDEIRRIVSERLQAVGLTGVEAKMPAELSGGMRKRVGLARAVALNPEIVL
                     FDEPTTGLDPVMTEAINRLIIDTQQRFQLTCVVISHDIQSIFEIGHRIAMLYEGKIIA
                     HGSPDEIRTSANPVIGQFLSGSIEGPIKIV"
     gene            complement(45977..46756)
                     /locus_tag="PLW16_04325"
     CDS             complement(45977..46756)
                     /locus_tag="PLW16_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB70164.1"
                     /translation="MNAISAALDGLGKAVLSYVEEMGRILLMFLSVGAWMFRPPLKAR
                     NIFKQMEFVGVKSIFVVVLTGTFTGMVMALQGYHGFRMFGAEALVGSTVALGMTRELG
                     PVLTALMVTARAGSAMAAELGTMRVTEQIDALSVMAANPIKHLIVPRVLAGVFMVPLL
                     TVVSDFCGIVGGYFVGVHLLDINSGIFIKNITKLVDLGDIYNGLVKAACFGLILSLIG
                     CYKGFNARGGAEGVGRATTEAVVLAAITILISDYFLTAIMF"
     gene            complement(46757..47824)
                     /locus_tag="PLW16_04330"
     CDS             complement(46757..47824)
                     /locus_tag="PLW16_04330"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPB70165.1"
                     /translation="MESTGKGKLKVGVIMGGISSEKEVSLESGRNIFSKIDRGAYEPV
                     PIFMDSRARLWEIPLKLLMRNSTRDIEEDLETEATPVPYEALKARVDVVCLGLHGKYG
                     EDGCMQGLLELLKIPYTGSGVLASALGMDKYASRKVLALGGIDVPRTAPLEIADWSAG
                     RQEEILADIEGRIGYPCVVKPAREGCSTGVEKVSTREALAAALTEAFRWDRQALVEEC
                     LAGMEVTCGVIGTDAPRALTPSETIATAEVLSLEDKFLYGQGENKTPARLPEETLTRI
                     QDVAVRTFRALDLRGYARIDMFVLADGRVVVLEPNTLPGMTPSTVLFHQAAASGITQA
                     GLIEAIIAAAVEVHANKRGPL"
     gene            complement(48103..>48992)
                     /locus_tag="PLW16_04335"
     CDS             complement(48103..>48992)
                     /locus_tag="PLW16_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70166.1"
                     /translation="VSQNTTTDIVERLETEARRIPYEGLAREIDFAFISLHGKYGDDG
                     CIQGLLELLRIPYTGPGVLASALGMDKHLQQKILRAAGIDVPDSLLIREEEWRRDRTA
                     VKKRVCEHFPFPFFTKPIREGSSVGVAVIRDGNDLDKGIEAALEWDRAVLVEEFLDGI
                     EFSSIVLEEDGVARPLEVTEIMPQSEYFTYDDKYMPGRCRKFTPPKNIPADRVERIRT
                     EVLRAFRALGFRSYGRIDGFYLADGRILITDPNSSSGMAPSSFFFEQAASSGMLPSMI
                     ISRLIENARRIHQEKFGPL"
BASE COUNT         9134 a        15871 c        15147 g         8740 t
ORIGIN      
        1 tggcaggtct acaacaccga gttctcctcg ccctttcgag agacaaatta cgagccggaa
       61 ttcctcctca cctatccgac caacatagac ctctgggcgc tccaaagccg gtttattcag
      121 gttggcatca accaccaatc caatgggcgt ggcgaaccgc tttctcgaag ctggaatcgg
      181 tttacggcaa atttcggttt cgagcggggc gtcttcaaca tgatcctcaa gacctgggta
      241 aggatccccg agaaagacag ccaggatgac aattcggata tcacatccta cctcggctat
      301 ggggagctct gggccggaac gatctggggg aattaccatc tccagctcat gttcagaaac
      361 aatctccgtt tcgacaagaa ccgcagcgcc ctgcagtgtg aagtgagctt ccccttgctc
      421 gagtacctca atgggtacat ccagtatttt gtcggatatg gcgagagtct catcgattac
      481 aaccactata ccaaccgaat cggcgtgggt gtcatggtga aggactggta gtgtcgggga
      541 atgggccccg caaaatcatc gcaaagacat ccgccccgat gcggcctgct tcccattgac
      601 ggacaaccgc attgccccgc tatttgcggc ccggattatc ttgacacgaa cggttgcgtt
      661 tcctatactg ccgccatcgt ccatcagact gccttattgc ccgacgggaa tgaatatggg
      721 aagcatggcc cacatccatg aggcggtcac atcttcaaca accaaagcga cagacgtgaa
      781 tccttgaaga acggtcaccg ataacccgta attgaggagg cgcgatttgg ctgactatcc
      841 ctggttcagg aactacgatg cgggggtgcc gcgcaccctc gcgccctatc cggacaagac
      901 cttgctggac gtcgtggccg aaaccgccgg acagcggccc ggccacacga tgatgtggtt
      961 caaggggaat gtcatcacct atgcccggtt tcagacgtac gtcgaggatg tggcgcgggc
     1021 gctcgccggt ctcggcgtca agaggggtga tcgcgtggcc ctgctcatgc ccaacatccc
     1081 ccagataatc atctgccagt tcgccgtgtg gaaggcgggc ggcgtcgccg taccggtcaa
     1141 ccctctgtac agcgaatccg aacttgtgca cgccctgaag gactgcggcg cggagatggc
     1201 cgttgtcatg accccgtttt acggccagct caagaacatc cagcccaaaa cccgcgtcaa
     1261 gacggtgatt gcaacagggg tgaaggaata cctgacgccg ttcaaacgcc tgatgttcgg
     1321 actgctgatg gaaaagaagc agggccatta cgtggaactt caggcgggcg acctgtggct
     1381 acaggacatc atccggcagt acgccggctc gccgtcgccc gatttcaaac tcggttttga
     1441 cgagaccggg ctgatcctgt tcagcggggg caccacgggc gtccccaaag gggtcatgct
     1501 ctcccacggc tgcatcctga tgaacgggat gcagacccgc gcctggttcg gaaagctgct
     1561 cgtggactgg gaggatgtga ccattctgct gatgccgctc tttcacatct acgggaacat
     1621 catcctggtc tcgaccttgc tgaacgcccg ggtacccatc gtgctggttc ccaatccccg
     1681 cgacgtaccc gacctgctcg cgacgatcag gaccaccaag cccaagtttt tccccggcat
     1741 tgccaccttg tttatcggcc tcatgaatca cccggacgtg aaatcgggca agatcgattt
     1801 caggtccatg aaaatgtgcg ttgccgcggc cgcacctctg ctgccggaga ccaagaagag
     1861 ttgggaggcg ctgaccggcg ggaaactcgt cgaggcgtac ggtctcacgg agtcgggcat
     1921 catcgccatg ggccccatcc tggggaaatg gaaggaaggg gcggttggac tgcccacccc
     1981 cgatgtgcaa gtcaagatcg tcgatatcga aacggggaaa aaggaaatga ccgttggcga
     2041 gacgggcgag attatcgtcc gggcgccgca tttgatgcag ggctactgga acaaccccga
     2101 ggccaccgcg gagatgcttc gcgacggctg gctgtacacc ggagacgtcg gacatctcga
     2161 ctccgacggc tatctgttta tcacctcgcg caagaaagaa ttgatcaagc ccagcgggca
     2221 ccaggttttc ccggtcgaag tggaggaggc catcgccaag catccggcgg ttctggaagt
     2281 gggcgtggcc ggcgtcaagg atgccgcgca gggcgaggcg gtcaaggcct gggtcgtgct
     2341 gcaacccgat cagccgtgca cggctgagga actccaggcc ttttgcaagg aacgattgac
     2401 ggcctataag gtgccgaagc acatcgaatt ccgcgattcg ttacccaagt ctcttatcgg
     2461 gaaggtgctg agacgcgtcc ttcaggaaga ggaactgaag aaataaagat ccgtcttcag
     2521 cccaagtacc tcgatacggt cgccgcggac tttccagagg tcgccttcat cgttgctcat
     2581 atcggcccgg gcgggtacaa ggaggccgtc gatctcgctt caatgaaacc aaagcacccc
     2641 ctgacgggcg cagcaaggct tggtctgttt ttcccgcaat taatatccct tgactttgtc
     2701 tggaatgagc atatcctgta tcccacatag tgattggcta agaatgatga gtaatgatcg
     2761 atcccacacg aaaagaagga aagttgactt acgaaccctg tcaataatga tgtgacaggg
     2821 ctttttaaat gggcttgaag ttcaattcaa gaaataccga aaatcaataa ggaggggaaa
     2881 gatgtcagaa ggaaaagtaa agtggtttaa tgataaaaag ggatttgggt ttatcgagga
     2941 ggacggcggc aaggacgtgt tcgtgcatca ctcagccatc cagggagacg gattcaaatc
     3001 actgtccgag ggtgatcggg tcagttttga tgtcacccag ggcgccaaag gtccggctgc
     3061 cgagaacgtt cgcaagctgt aggaccttat ttgagggtca ttgatctttg acacgtttta
     3121 ttccctcgaa atgtgagcag ggaatatccc gattagtgta ttcaacagac gagatgctgc
     3181 tgtgtgcgta ttgagtcact ctactggttg ttaacgaaaa agagaatctg atggtcttcg
     3241 tggtgattgt cacccttatg gaccggatct tgagtgatgg actccagtaa aagacgagac
     3301 ggcacaggcg gctatcgtct ttttttttaa tcaggacatt ccgacacatt tgaaggatgg
     3361 atcagaatcg gcactcctgc ccatagacca tctcgcgggt gatggttggc ttgtttaaag
     3421 atcacgatga tctggtcatg ctggtgacgc ctgaaggcac acactctttt cgcaccaaat
     3481 ggaaaacagg cttttatcgt ctggcggtct tagccaatgt ttctaaggag gggaaatatg
     3541 ggatccaaac agaagcgggt acaaatcgga caaaaagaat ctttcggacg aaagctgaag
     3601 gcccggctgg ctttactgtc ggaaagggga atcaaatctc atgagattga taaagatccg
     3661 ctcgtaaaaa atttgcgggc taatattcgc gccatcaatg tcaggatgca ggcctttgaa
     3721 gcgagcgaaa agaagaacga agccctggcg aaaatgaagg cggataaagc agcgcccccc
     3781 caagaaagtc cggaaggaac gaaaagaaaa aaggtgaagg aaccccccgc tgaaggcaag
     3841 gagaaaaaga aaaagaaaga atagttcttg aaagtgataa aaaaccagca agaaccttcg
     3901 ccggggtgac gtaagccttg atctgcattc agaatctcag cctgcgccat acatgaactc
     3961 tctgatcgaa tggaactgat ccaagtgcac cgggcacttg tcggttctaa atacacgata
     4021 aggaggattg tatgattgtc gatagccatg tgcatctttt ctatgagaat tccgatccca
     4081 tgtcctggtt tataggaagc gcgagaaccg gggcggccac gttcgccaaa agtaccggag
     4141 agctgggaaa tgttgaagag ctctaccaac agctcgcccc cgagatcatg gatgataacg
     4201 gagacaaact ggtgccgagg atggatgacg cgggaatcga acgggcaatc attctcccga
     4261 ttgaccttcg cttgctcagc gatgagcctt ccgccttcga tcagacctac cccaccatcg
     4321 agcagaagaa ccgaacctac ttcaaggcga cacagaggca caagggacgg atttattcct
     4381 gcgcgggagt cgatccacgg cgtcatggtg cggtcgatct ttttaggaag ggggtggaag
     4441 aatgggggat gatcggactc aagcttcatc cgactgcggg gtactatcct catgacccta
     4501 aatgctatcc aatgtatgag gctgcccggg agatgggagt tcccgtgatc attcattccg
     4561 gaagcgagcc ggcaccgttg aagtgcgcgt actctcagcc caagtacatc gatacggtcg
     4621 ccgcggactt tccggaggtc accttcatcg ttgctcattg cggccatggt tggcacaaag
     4681 aagccatcga tctcgcttcc atgaaaccga atatctactg cgatttcagc gggtggcagg
     4741 ttgaatatct cacgaatccc gattatttct tcggaccttt acgttatgcg ctggattttc
     4801 tcggaccgtg gagagtcctg ttcggcaccg atgggccggc ctataccgtc tttatgagta
     4861 ataaagactg ggtaaacgcg attcgcgaac acaaatcccc ctccggaata aaattctccg
     4921 acgaagaggt cgacctcttt ctgggcgggg cggcggcaaa gctgtacggc tggctctgat
     4981 tatttcattc aaaaaacacc caccgtacgg actccccccg gcatgcgcgg tgccgccaag
     5041 ggaaaagtgc tttataaaac cagatgacgg gaggcgtatc acggcgctgg ccccggttga
     5101 aggcaccgcc aacggccggt atctcgtcaa cggtttcttc gaatacgtcg gcctggtcgc
     5161 ggatcccttt ctcctgatca tcaaggacgg cagggttgtc gaagtccgtg gcggcggtcg
     5221 cgaagcgcaa aaagtcaggg acatcattgc cgcggcggat acccatggaa acaacatcgg
     5281 ggaactgggt gttgcgacca accatatcaa gatctttgac ggtttttcag gcaccatcat
     5341 cgacaagatg attcttggaa cgatccacat tgccatcggg aagaatacaa cgttcgaggg
     5401 aggcaaggtc tacagcaaca tccaccacga cgcggtgagc gaggggatga cccttaaagt
     5461 cgacaacacg acgatcatcc gggacggcaa attcatgttg gattagcgtc cccctgactt
     5521 tgcctgaggt ccgatgcgat cgcctgacgg cccttcttga caccggcgcc cgattcctct
     5581 atacgttcgg caactttcac cggggtgacg tcagccttga tctgcattca aaatctcagc
     5641 ctgtcgttcg gggacaaggt cctgttcgat cacctgacct ggaccatcac cgagaggagc
     5701 cggatcggcc tcgtcgggga caacggcacg gggaagacga cgctcttccg gaccctgctg
     5761 ggcgagatcg accattacga cgggacggtc cagcttccga aaaaccggac catcggctat
     5821 ctgcctcagg acatcgtctc cctggagccc gtgacgatgt tcacctacct gaaaaagcag
     5881 agcgggatcg accggctgga gcgggacctc gcggcgcagg aggccgcgat cgcccaggcg
     5941 gaccccaaag gggcggccta tccggccctg ctccgggcgt acgaggaaac cctcgcccgc
     6001 ttccagtcgc gcgacggtta cgccttcgag gcccgcgccc ggcagatctg ccggggcttc
     6061 ggcttcgggc cggacgattt cgacaaggac tgcacgacct tttccggggg atggaagatg
     6121 cgcatcctgc tcgccgccct cctcctggcc cggccggaca ttctgcttct cgacgaaccg
     6181 acgaatcacc tggacacgga aagcatggaa tggctggagt cctacctgag ggactatccc
     6241 gggacgatga tcgccatcgc ccacgaccgc tacttcctgg acaagctggc gggccagatc
     6301 gtcgagctgg cccagggacg gatcacggcg tacgccggca acttctccgc ctacctcacg
     6361 gaaaaggagc ggcgcctcga ggcgctccgc aaggaaatgg cgctgcagcg ggcggagatc
     6421 aagcggaccg aggccttcat cgagcgcttc cgcttcaagg cgaccaaggc ccggcaggtc
     6481 cagagccgga tccgccagct cgagaagttc tctccggtgc aggccgcggc ccatgaaaaa
     6541 acggtccgca tccaatttcc cgccggcccg cagagcggcc gcgaagtggt gaccacgcgg
     6601 gatctcgcgc atcgctatgg ccggaacgag gtcttccggg atgtcggctt caccgtccgc
     6661 cggggggacc gcctggcgct cgtcggggtc aacggaagcg gcaaatcaac gctgtcccgc
     6721 ctgctcgccg ggatcgaggc gccggcggcg ggcgagatcc gctacggcgt ccacgtccgg
     6781 atggccttct tctcgcagga cggcagcgcg aacctcgacc ccgcgcggac ggtctgggag
     6841 gaggtgcagg cggtcagctc ccaggacgac gaccagcgca agcggaacct cctgggcgcg
     6901 tttctcttct cgggagacga catctacaag cccgtccggg tcctgtcggg cggggagaag
     6961 tcccgcctcg ccctgctcaa gatcctgctc acggaaacga acctcctgat cctggacgag
     7021 ccgaccaacc acctggacct gaaaaccaag gagatcttcc aggaggccct gctcggttac
     7081 gccggcaccc tcgtcatcgt gtcccacgac cgctttttcc tcgaccgcct cgccaaccgg
     7141 gtcatcgaac ttcgggaggg acgcgcccag gaatacaacg gcaactattc ctatttcatc
     7201 gagaagcgcc gggaggcgga agacggcgcg gcggtaccgg gctcagccgc ggtgaacggc
     7261 gcaccggcgc cgaaggacgt ccgtcagagg agcaaggagg agcggcgcct ggaggcggag
     7321 gagcggaacc gcctgtaccg cgcccggagc ggcctggagc acgacctagc cgcgacggag
     7381 gagcggatcg cccgcctgga ggaagagaag gccgcgaacg aggccctcct ctgcctgtcc
     7441 gaaacccacc gggacgcccg gaaggtccgc gacctgagcc gcgaactgca ggcccttgcc
     7501 gccgagctgg aggacctcta cgcgcgctgg aacgacctga cggagaagct ggccgaaatc
     7561 cgctaggccc cgtcaaggcc gccgcggtcc gtcttgcgga accagccggc cgggtccgtc
     7621 agatactcct cggtgtcctt gagcgacgtg tagtgctcgt gctcgatgga ggccaacaag
     7681 tcgaagaagg ccttctcccg gggatcggtg acccgatccc ggagccccgc gtagaattcc
     7741 gtccccctgg cctcgaacgc gatggccgtc ttcagggcct cgcgatcgtc ggcgtcgccc
     7801 gccggggcgc cgtccgcctg cttcaggacc cccttcagaa cgtccttcac gaccgttccg
     7861 ttgaccttca ggggaacggt ctccggccat tgccggttcg cctcccaggt ctccttgagc
     7921 gcgtgcaggc gctcgtaatg ctccagttcc tccgccgcga tctgccggaa catggcggcg
     7981 ccgacgggat tcttggtgcg cgtggcgtgg cgcagataga attcccgctc cctgagttcg
     8041 ttgttcaggg ccacctccag ggcgttcaaa cgatccttca tggctgtcct ccttctgttc
     8101 gggctcgagg ccgattcggg aaaagcccga gccggcggac ggggcatttt cccggactcg
     8161 ggcgtcgggc gccatgataa tggccccggc ggcgattgtc aagcggggcg gcggcgcggg
     8221 tcgggcgttc cggttttttc gttgacatcg cggggtgggc tgcttatact ccccccaaat
     8281 ttccaggggc gggtcaggcc cgggcgggcg gcggcgatgt tcaagtcatt ccggcagaag
     8341 gtggggttcg caaaaaggag attcatcgag ccgccctatc cgaaacgagc ggacggccag
     8401 gtctacctcc acatcgggtg cggcaaaaag aaccaccccg gcttcatcaa catcgatgcc
     8461 cagccgtttc cccacgtcca cgtggtgacg tcggacatcg cccgtctgga cgcgttcgcc
     8521 gacggatcgg cggacctgat ctacatgagc catgtgctgg agcatttcaa gcgggcggag
     8581 ctcgacggca tcctcgggga gatgcaccgg attctgaagc ccggcgggag actccggctt
     8641 tccgtgccgg atttcgacaa gctgctggag gtctaccggc tttccgaaaa cgacgtccgg
     8701 tccatgcaca cccagctgat ggggtcgcag gaccatccgt acaacatcca ctacaccgtc
     8761 ttcaaccggc gggacctcga ggcgctgctg ctggccgcgg gcttttcctg cgtgcggccg
     8821 tgggatccgt gggacggaag cgaaccgcgc gtgaaggacc gttcgatgaa aatcatgaag
     8881 gccgggaacc aggcgatcaa gatcagcctg aacgtcgagg ccgtcaagta ggcgtgccgg
     8941 acgacttgcg gtcgcggggc gattccatga gccgacttct ggttctcacc aacaacgcgg
     9001 agcgggccag cttccgtcag aggatcggcg cctacctcgc gccgatgcgg gacgaaggca
     9061 tcgaggcgag cgtcgagatc ctgccccggg ggatgtgggc gagaagccgg ctctttgcgc
     9121 gggcgggggc gttcgacggc gtcctggtgc acaagaaggg cctgaatgtc gtcgatgccc
     9181 acctgcttcg gaaacacgcc cggcggatca tctacaacta cgatgacgcg gtgatgcaca
     9241 gcgaccgccg gccggagcgg cgcactcgct cgcatttcgt tccttggcgg aggatggttc
     9301 gcctgtccga cgccgttatc gtcggcagcg agcacctggc ccggctgggt cgggcctaca
     9361 accggcaggt ccatgtcgtg ccgctcggcc tccgggtcgg cgactacgcc tgcgacgcgg
     9421 cgaagcccgg agacgggaag gtccggctgg tttggatcgg cagcgcgagc acgctcgatt
     9481 atctcagggc gctgaagccg gtgctcgagg ccatcggcgc ccggaggggg aacgtcgtgc
     9541 tgcggcagat ctgtgagtcc ttcctggacc tggattccat gccggtcgag aagctcgtct
     9601 gggacagcgg gaagcgcggg gaacacctgg gaacggcgga tatcgggctt gcgccgctgc
     9661 cggacaaccc cttcacccgc ggcaagtgca gtttcaaggt gctggagtac ggcgcggccg
     9721 gcctgcccgt ggtggcctcg ccggtgggca ccaatgccga gcatatcgtg gagggcgtca
     9781 cgggccttct ggccggcagc ccggcccaat gggaggcgca cctggtgcgc ctcatcgagg
     9841 atgcggccct gagggagaag atggcccggg ccggccgtct gcatgcggcc gggtacgacg
     9901 tgagcctcat cgccccccgg ctcatatccg tcatcgcgca ggccatcgaa cgatagaggc
     9961 ccgggcagaa agccgcccga cccgcaacga aaaccatgac gaccccctcc acccccctgc
    10021 tgctcaggcg cgtcttcccg caccgcgccg atgaaacgat ccgatgcacc cgtctgctcc
    10081 gatccgtccc cggcaagcgg gaggttttcg acgcccggtg gaacgaccgg ccggtcgtgg
    10141 tgaagatctt tttccatccc tgccaggccc ggcggcatct gcggcgggaa tggcgcggcc
    10201 tgaacgccgt gatggcccgc gagctgacgt cccagaaacc gctgttctac ggccggacgg
    10261 acgcggggcg ctgggccctg gtcgtcgata aaatcgtcga ttcgcaaccg gcgacggacg
    10321 tgttcgcgag cctgccggaa ggcccggagc gggtcgagct gctaaagaag gtctgccggg
    10381 aactggcggc gtatcacgac cggggcgtcc tgcagcagga catgcatctg ggaaatttcc
    10441 tgctcaaagg cgagcgcgtt ttcttgctgg atgccgcgcg gatgaaattc cgctcccggc
    10501 cgatttcacg cgccgagggg attgcccaag cggcgctgct cagcgttatt ctgacggacg
    10561 tcgatgcggg cgacatccgg gagctgtgcc ggagctatct cgacgcgaga ggttgcgccc
    10621 cctccccggt cgatgaagcc cggatggcct ccctgcggag ccggtaccgg agtgtcgccg
    10681 tcgaacgggg gctgaaaaag accctgcgga ccagcaagcg gtatcagcgc gtcgcgggca
    10741 aaacggtccg gggcgtttat gaaaaggcct tcttcgggaa tgccgacctc cggcccctgt
    10801 tcgaccggtt ggagaccctg acgggcgccg gggaagacgc ggggagcgga aaggcccgtg
    10861 ccgcgtcgat cttgacctgg cacgggaaaa agctggtcgt ccagcgctac cgggagcggg
    10921 ggttgatccc ggcgatccgc cggactctca cggcctcgca cgcgaagagg ggttggctga
    10981 acggctatcg cctgatcatg ctggggatcg gcacgccccg gccagtgtgc tatgtcgagt
    11041 tccatcgggg gtgctttgtc cgggaatcct ttctgatcac ggaatgcatc gaagggaaaa
    11101 ccctcgcgga aacgcttcag gaccgcggcc tcaccgcagg tcggcgtgaa accctccttc
    11161 agcacgtctg gcggcaggtc catcgcctgc atgaacaccg catcgtccat ggagacctga
    11221 aacagggcca tatcctgatc tccgcccagg gaccgctctt gacggacctc gaccggatga
    11281 cggtatgcgc gtgggactgt ctgttcaagg cctggcggga ccgggatctg cagcggctca
    11341 acctcgacgg accgggggag gagaccgacc gggtctgaaa agaggcccgt ttcctccgac
    11401 gtgagtagcc ggattgcgtt gggccttcgc gggagcgcgc cgcccggacg attaagggaa
    11461 gacccggggg aaggctcagg cgtcgggggg ggccggtctc ggcttgacgg gtttcgccgg
    11521 caccccgatg cagatcatac ccggcggcag gtccctgctg acgacgctgc cggcgccaac
    11581 caccgtcccg tcgccgatcg tcacccccgg cagtacgagg gcacccgcgc cgatccagca
    11641 atcccggcca atccggaccg gcgccggaac ctcgggcctc atgtccagcc ccctccgatc
    11701 gaggccatga ttggccgtca cgacggcgac gccggcggag atgagcgtat acgcgccgat
    11761 catgatgggg gcgtgcgcct gcaggatcac gtccgggccg atgctgacat ggtctccgat
    11821 cgtcacccgt tccggctttg tgacccggag tcctttcttc aggtcgacgc cttttcccac
    11881 ccgcatccgg gagcgccagt aggacagctc ccgcttttcc agccatgaaa atatgcgatg
    11941 ccccatcaaa tcaaacatgg acacgtcctt ttccgcatcg gccccgaccc ggatcgcgcc
    12001 ggggcatcgg ccgtcagcgg ttccgcctac ccgccgggat cgattctacc actagaaata
    12061 tcccttcagt gctgttgtgg ctaacgcact gcaaagcatc tgacaatccg tcatccgcga
    12121 acgctatttg ttgcggacaa aagagaccgg gtcaccgatg ttgttgatcc cttccaactt
    12181 ttggccgtcc ggagacaggg tcatcacatc ggtaaatccg tggttccaaa cgatggaaaa
    12241 gattcgtttg cgagggtcct tgcaggacca cttgccagac acggttatga tcagaaaacc
    12301 tttatagatc gccattccgt cgctgctgaa gtaagcttga ccgctgacat gtgtgggata
    12361 ccagacaccg gagatatccg gaaacgtagc agaatccagg cttgcaagtt tttccaaata
    12421 ttgtttcttt tccggataaa gagaaaagag tctctgatat atctccaggg cacgtttttc
    12481 attgttcttc atcgatgcaa attccgcctc attctcgtag gcggtcgtgg cgcgtttttc
    12541 taaatccaaa gcaacggaat gctccggatc gaccattcgc acctttcttg aatattcaat
    12601 tgcattatct cccggcggtt cagcgaattt cctttcattg aatgcttgtt gtgccaaagc
    12661 cagattcatt tgttttcttg actctcccgc gtttatagaa ggcggccttg ccgcaccagt
    12721 atcctgaaga tcagaagcag aaggagaggg cggagcttct ctttgtcccg ccggaacctt
    12781 ctgctctcct tctgcaaaat agaattcccc cttcagggat gacaattccc agggcgtctg
    12841 tttcatacct tcaaggctca cgcgcacctt tttgaatacc tgttctatcg ggagtccggg
    12901 ttcgttgatt gccttcaata atgcggcagt gtaagggctg ttgcgcccct ccccgtctgc
    12961 ggccgtctgt cccggtgacg tggaataggt gatgaatgtt ccttggggag catccgagat
    13021 gatcgccagc ccacgtccgg tgctccgcaa ggagcgtctc acgggattgt cacggcaggc
    13081 gtcgagaatc acgatgttca tggggttacc tgcattggcc atctcgtcaa ggaccatctc
    13141 tgcatcaatg gccttatact tggcatccgt ttctttttcg atccgcgccc cgatggggat
    13201 gaggtaattg cgaccattga tttggatggc gtgacccgcg taaaagaata aggccacccc
    13261 cccttttttc aggcggtggc caaaatcgcg gatggattct tccatctcct gttgtctgac
    13321 gtccttccgc aggattatct cgaagccaag gcgttgaagg gtggcggcca tgtctgtggc
    13381 atcgttgacg gggttcttca gggggccgga gctgtaggcg ctgttcccga tgatcagggc
    13441 ggttcggcgt tccactgctg ccggcaatgc ggacggaatg gccacagaaa gtatgaccag
    13501 aagaagggtg atttttgaac tcagagcacg catggaagca tcctccccgt ttccctaata
    13561 aattaatcct tcccacgagt caacagggat tagaaagacc cacggcttgt tgtattcagc
    13621 ctgttgtatt ctgttgacac ccggcggcgc tttccctata ctcgagccgc ccgcgaggga
    13681 caggcgacct tgagaccgac gcaaccccgg cgcgatcgcg gccccgggcg ggcagggcta
    13741 cggggggcgg aaaaaacgac agggaggttc ctcttcagat ggatccgatc agcgacacgg
    13801 cgcccagcga ttttatccgg gacatcatcg ccgacgactt caagaccaac cggcacgaag
    13861 gccgcgtcgc cacgcgtttt ccgccggagc cgaacggcta catccacatc ggccacgcca
    13921 agtcggtctg cctcaatttc ggcatcgcgg cccagtacgg gggcacctgc aacctccgca
    13981 tggacgacac ggacccggcc ggcgaatccc tggagttcgt ggaatcgatc attcaagaca
    14041 tccactggct gggcttcgac tggcaggacc gcctgttttt cgcctccgac tatttcgagc
    14101 agctctacgc ctttgcggtc gagctgatcc gggccggcaa ggcctacgtc tgcagtctcg
    14161 acgccgacga gatccgcgaa taccggggtt ccttcaccga accgggaagg gagagcccct
    14221 accgcaatcg ctccgtggag gagaacctgg acctgttcgc ccggatgcgg gccggggagt
    14281 tcgccgacgg cgagcacgtc ctgcgcgcca agatcgacat ggcctcgccg ttcatcgtca
    14341 tgcgggaccc cgtcatctac cgcatcaagc gggcgccgca ccatcggacg ggcgaggcct
    14401 gggtcatcta ccccatgtac gatttcgccc actgcctctc cgacgccctg gagcggatca
    14461 cccactccat ctgcaccctc gagttcgaga acaaccgccc cctgtacgac tggttcgtgg
    14521 accagttgat ttcctgggac catccccggc agatcgaatt cgcccgcctg aacgtgagct
    14581 acacggtcct gagcaaacgg cggctgatcg agctggtgga aaacggccac gtctcgggct
    14641 gggacgaccc gcggatgccc acggtcgccg ggatgcggcg gcgcggctac acgccggagg
    14701 cgatccggaa cttctgcgcg gagatcggcg tggcgaagaa cgacaccgtc gtggacatcg
    14761 ccctgctgga acactgcgtc cgccaggacc tgaacgaccg ggcaccccgg acgatgggcg
    14821 ttttgaaacc gctcaaggtc gtgattgaca actaccccga ggaccggacg gaaaccttca
    14881 cctgccccaa ccacccccag aaccccgtgc tcggatcgcg cgaggtcccc ttctcgcggg
    14941 ttctctacat cgagcgggac gacttcatgg aggacccgcc caagaagtac aagcgtctcg
    15001 ggcccgggcg ggaggtccgc ctgaggaacg cctatgtcat ccgttacgcg agccataacc
    15061 gggacgcggt cggcggcgag gtcacggagg tccactgcgc gatcgacccg gaaacgcgca
    15121 acgcgccgcc cgcggacggg cgcaaggtgg agggggtcat ccactggctt tcggcggcgc
    15181 acgccgcccc cgccgaggtc cggctgtacg accggctgtt ctccgttccc gatccggcgg
    15241 ccgccggcga cgatttcgtc aagtttttga atgcggattc tctggaggtc ctgaagggct
    15301 gcttcgtgga ggccggtctc acggaggcgg cgcccggcgc ccgcttccag ttcgagcgga
    15361 cgggctattt ctgcctggac gagaaggaca gccggccgga gaggctcgtc ttcaaccgga
    15421 ttgtcgccct gagggattcg tggagcaagg gccagggaaa ggcctgagcg ggggaagacc
    15481 gccccgggga acgcccggtg cgcctgggag cgcccgggaa cagcccggcc gggtcccgcg
    15541 catcgatttc cctcgcgggc gaaccgctca tttttcgcct tccggcgaac aggcgcggct
    15601 gtacttggag gggtggcgcc gcagggatgc ccgcaggtcc tccgcccacc gcgcgacgtc
    15661 gccggcatcg cccaggtaat gcctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnccgca ggtcctccgc ccaccgcgcg acgtcgccgg catcgcccag gtaatgcctc
    15841 aggaaaagca gacggtcttc gacaccgctc agcgccagcg cccgctcgtg caggtggtgc
    15901 aggtcccgca ggcgccgccg ccgcgacatc gccttccgga acaccgtgtc ctcgtagtcc
    15961 agaatccaga gggaaagccc tccgtcgcgc cggccgacca gcaggttgcg gagcgacagg
    16021 tcgtcatggc ccacgccggc gtcgtgcatg cggcggacga aaccggcgac ctgcccgatc
    16081 aggtcctccg tcagcccctg agcggcgatc cacagatcca cgcgttcggc gtcggcgatg
    16141 agttccgtga cgagcagcgc caggccggtc ctgccgtcca cggcccagcc cagcatggcc
    16201 ggcgccggga tgtcccgtcg ggcgagttcg agcccgtttt caaaggcgag gcgggccttg
    16261 ggaaagccca gccgattgag gcagatcacg cgcggccgat tgtcgtagaa gagcttcacg
    16321 cagccggtcc gccccgcgag ccggaccgcc aggacgccgg cccgctcgcc aagatgaatt
    16381 cggcggatcc cgtcatcgtc atccagggcc cagcgatgca ggcctcccgt atagcgatcg
    16441 aacaccgtcc gggcgagccg gcggagccac tccccgtcct ccacgtgggc ggtatgcacc
    16501 gtgagccgac cgttcatctc cgcctccgac cccggacgtg cgggattcct cccgcctcgt
    16561 cccccttcgc gggggcatga aagaccgtca tgcccccacc cggaccgtca atctgccgtc
    16621 gtcttatcca acgcctccac tgccgcgatg ccccacgagc cgcggctctt ccgggggcgc
    16681 actgactagc cgcctgcctg cgcactccgg tgatccgccc gcgtcgaacc gggcggctat
    16741 ccgatacggc ggccgctctc ccggtcggcc tgcttgcgcg cgttgacctt gcgcatatgg
    16801 cgcgcgatac gcgcgatctt tcgttcgacg agaagcagca aaaaccggtc caccggccgt
    16861 atccgcgcgg cgccgcgaaa ggcttggtaa agcagccgac ggtcttcccg gtcgaagagc
    16921 ggctccggca gcgtgaagaa cagctccgcc aaaagcttaa cgcgccattt catccgcagc
    16981 cacgtcctgc ggtccacccg ctgaaaatcg aacagggcca ggaaaaccgc ccccgtttca
    17041 tccccgggac cgatcaggat gtgggtggcg ttgaagtcgc ggtggttgaa cccctgggcg
    17101 tgcatcgcgg ccgccaggcg gccgatggcg gaaagcagcc gccgcttcca cggcgtcccc
    17161 gccggtccgg cgaaccgctc cgggcgctcc cggatcagcc gttccagaga aacgtagggg
    17221 gcaaaatcct ccgtcacgag aaacgactcg aaccggaacg gaccggtccg ccgctcgccc
    17281 gcggcgacgg gcgtgaccgt gggcaggccg ctctttctga aatcgcggat attctcgaat
    17341 tccgccatcc cgggtgagag gccgcgcgcc gagaacccgt ggccgatcca ggccccgagg
    17401 tccggacgca cggcccggtg ccgcttgagg aaaatccgcc gcggccgccc gtcctgaacg
    17461 agatcgagtc ggaccacgct gcgctcctcg atcttcttga cgaccgtgcc gccttcccgc
    17521 cggaagatcc gttcgtaatc cagtagatcg ttttcggcga ggagggactg gaagtcggcg
    17581 ttcacgacca tgtccggccg ggtcacgatc ctgatcttcc cggcgccgtt gctcatgacc
    17641 gcttgtcctc gatcacgtcg gcgtaaaggg ccagcagatc gcgcatatgg gcctccatgg
    17701 tgaaggaggc ggccttttga caggcccgcg ctcccaggac gcgtgggtcg tcgacggcca
    17761 gatacgcgcg cagggatgcc gtcagctccg ccaccgagac gtcccgcaag acaaagccgt
    17821 cgcgccccgt ctcgatgact tcggacacac cgttcatccg ggtggtcacc accggggtcc
    17881 cgcaggccat ggcctcgagg cagacattcg cgaagggatc gtatcgcgtc ggcagcgcga
    17941 acaggtccgc cgcgccgtaa aacccctcca gcgccttttg ataccccacg aacacgacgg
    18001 cctcccccag accgagccgc cgggcgcggg atcggtatgg atccgcggaa tcggagccgg
    18061 ccacgatgat ccgtatattc ggggcgccga ggccggccac ggcctcgagc aggacggaca
    18121 ggcccttgag cttgaattcg tttcccgcaa agaggatcag ccgttcctcc ggcccgatgc
    18181 cgtagcgtcg acggaggggc gcaccgagcc gccccctcac gttgggattg aaccgcgacg
    18241 tgtcgacgct gttgtagatg acgcggaccc tgtcttcgga aacccggtag tggcgaagga
    18301 cctgatcccg gaccaggcgt gaattggtca tgataaagcg ggcgccgcgg cgctcgaaga
    18361 tccggcgttc gagaaacgtc agcgcccggc gccggggggt cacggccttg aaccgccgga
    18421 gccaggggtt ggggtagcgc tgggccatct ggacggggtt gatgccgtcc gaggcgcgga
    18481 agatatcctg gcgccagatc cgctccatgc tctgaaccac gtcgaggcga agcggcgcga
    18541 tccgccgcga ggccatgaag gcgaaggaga ggtttttccc gggggaggac agggggaaaa
    18601 acggaacggg gtggaaatgg aggcccggga gccgttcgcc gctgtgggcg aacacgtgaa
    18661 cgtcgtggcc ccggcgaagc agttcagccg ccagggtgac ggtgtaccgt tccagcccgc
    18721 ccttggtgga gagaaaatgg cggcagacga gcccgacctt catcgggaga cctcgtagag
    18781 gatcagggca ccctgctttc tggaaggcca ccgctcaagt tcgcgaaacg tgcaggaggc
    18841 cgaggggtgc gagaaatcga ccttcgattc ccgccacccc ttttcgtccc ggaccagata
    18901 atcacagggc gcgcgacaga tcgaatcggc gctcagggca ttcgcctcat ggaacagcgc
    18961 cctataatcg gaaaacgtcg ctgaaggcgt gttcaggttg gcatagaact ggacgtccac
    19021 cacccgctta aaggccccac aaacgttgac cggccgggtg ttcgcctcgc gttttgcgat
    19081 gtaacggccg atttcccggt agaccagttt gtcccgatcg aagttcaccc acaggaccgg
    19141 cggcaggagg gtgaggagca ccaccgtgcc gaccagggca taagcggcgg ccggccgata
    19201 ccggaagcgc cacctcagga gggcatgacc ggcctcgatc cccgccccga tgaaaacgaa
    19261 cgacgggagg atgaacacgg cgagatggcg cgaagcgccg tgccaggtat agagcgtctg
    19321 taaataaagc gcccccagcg ctagaatcga caccaccgcc aggtagcgga tgcgcccgtc
    19381 cgcccatcga cggcggagcg atgaaaccgc acccccgatc aggacaacga agaaaacata
    19441 gaacagggtc tcgacgatca ggacgaccgg ggccaccagg gcgatgatcc acaggaggct
    19501 ccggatccgg tcgaaaaaat agctttttat cccgatgatg taggccggtc gggccaggat
    19561 tttcaattcg tcgcgaagcg catcgtattg agcaatgatg ttcaagggaa gattcaggat
    19621 tcgtttcgga ttgaggatct cgaccaggct gagatccaga tagaaagaca ggcccaggaa
    19681 gagagacccc aaaaaggcat agggaagccc gaaaaccagc aggtccctcc cccgtgcggg
    19741 tcggttcaaa aagaaaatcc agccggcggt cgcggcgatg taaagccccg cctcgaagcg
    19801 cgcccaggcc gcgagggcca ggcaaaggct gcagcccagg agcagcaggg gccgccgctt
    19861 ctcgatgtgc aaaatgaaga ggtacagccc cagggccgaa aaaaaccaga acatcggatc
    19921 ccgcagggcg tccctggaca cctccgcgaa tgtcggcaga aaggcgaaga ccagggcgcc
    19981 gagggcggcg acgccatccg cgaagaacct gcgcaacagc ccgtaaagga cggcgatcgt
    20041 cagggtgccg aacaggatgt tcacaccctg ggcggcgacg atccagtttc cgcaaacgga
    20101 atagaacagg gagaccgtga tcgggtaggg agatacgtag atatagtaag cgagcaattg
    20161 atcgaagaga ccatagtaga gcgccttggc ctgctgaaaa tagaggtggc tgtcggggct
    20221 caaggtggga agccgaaaag caaggtagat cctgacggaa aatccggcga gcagaacaca
    20281 ccaggcggcc ggagacagcc tccacgggga gggaaaatcg gatggaggga cggagggcat
    20341 ggaggggttc ctctcagggt ctgactttgt cggcatggat gaagatcgcg tttgcccaga
    20401 tcacctggcc gccggtccgg ttgtagccgg gcttgtagat atcgtagacc gaaaacccct
    20461 gctcgcgcag gaaaagatcc agctcgccga acaggacgca cttttcgtac gctccattga
    20521 accagatctc gctgtagacg atcagcgtcg attttctcag ggttcgggtc gcccccttca
    20581 gggcggtcag ctcatacccc tgaatgtcga atttcatgaa ttcgatcggc acatcgccgt
    20641 ttcgctccac ccagccgtcg atcgtcacca gcggcacctg aacgaccttc cggattccgg
    20701 cgccgtccgg gtcgatgtcc cgaagcctgc cggacggggt caaaagggac gcattgccgg
    20761 ccgaggccgt gatattgagt tcggccgtcc cctcgtggtc cgaaagcgcc gcaaactggg
    20821 gatggaaccg gggatcccgc cgggcgtagt cgatcaggcc ctcccggtac atcggattcg
    20881 gctcgaaggc ataggcgtgg gccgagggga agttcttgag gatccgtttc gaggcatgtc
    20941 cattgctcgc acccgcgtcg agaatccctc gaacggcgtg accccgcaaa agtgccctga
    21001 cgacctcgta gggatcgtcg agcgaaacga ggttctggtc ctttttgagg accttctttt
    21061 tcaaatacgt gctgaacctg ttgggcatcc gacggtctct cccttcaacg cggcatccgg
    21121 aaggccccct cgatccggcc tgccgccctt ttcccgcact cggcctcccc gtttccttgt
    21181 ctccgtcccg ccgctcaaac ccgacccgtt ccaaacggcc ttcccggccg cctcgagacg
    21241 cggatcggct gcgggaaacc ggcaccggac gcgcgcctac cggcagcgga ggcgggcgtc
    21301 gaacactggc cggatggttt gttcgcccag ggagttcagc tcgttggcac agaaggcgag
    21361 gggattggcc ttgcggaact ccttgacggg gggactggac aggattctct cgatgtcgag
    21421 cagcaaggcc tctttccggt cactcaaacg ctttttgtgc agataacgct tcttcaggca
    21481 gtacttcagg aaccgctccg ccttgtcgga aagataccgg cggtcccgca gcgcggggtc
    21541 gaaatcgggc agggcgcggt cttgggccag acggttcatc tccatgagga tcccttccag
    21601 attctgaagg aaaagctctt tccggaagag gaccgtccgg gcggtctccc gcttcatctc
    21661 cgccaggcgc ttcaggtagt aggccgccac ctggctgaag gtgatcgtat gaagcccctg
    21721 cagaatctcg tagagcgtcc ggttgaattc gtccttgggc ttgcggaaga agatcggcgg
    21781 accgacgcgg atgaacacgt ccttcgcccc ccgcacgagt cggtccacct tgacgttgat
    21841 gggcaggatg tacaccttcc ggccggtctc ctggaaggtg ttttcgagga cctggtacac
    21901 ggaactgcgg attttcttca ggccgccgaa tccccatgtg gtcccctcgg gaaaaatcag
    21961 tgtcgaaacc ccctgagcgc tgccttccct gaaccggctc aactgatcgg agatttcctg
    22021 cctcaactcc tcctttttct tgctcaatac ctcgacaaag ggccggtcga tggccgccaa
    22081 tttcagtttc cggaaaacgg ccatgggcag gcccgacttg tcgatcatca gcaggatgct
    22141 tcgcagcagt gggctccttt taccgtagtt cgtggagaga tagcggccgc tcaggaaatc
    22201 gttcttggcc atgaccatga aatccggccg gggtttcagg gcatagtaga cccgggagag
    22261 cgacggaacc tcgaaatagc tgtcgtgggt cacggcgaac atgatcgggg tcgggatgcg
    22321 cgcgaggttc tctcgcccca gcagatgcgc cttgtggcgc atgagtttcg aggccaggta
    22381 aaacccgaac agaatgaatt cataaaagac gccgcgatgt ccggacttca tccctgacgc
    22441 accctcccct tttcaaaaca tgatggaagc tatatcgcag tcccctcatt ttttttcaag
    22501 atctttttcc tcgtctcgcc ggcgccggga cggggcattc cggcgggccg gcgcgaaaat
    22561 accatttgct attttacccc gcgctgcata tattacgccc gcttccgtaa ctcgggttcc
    22621 agcgggccca aagtttcacc ccgctgccgg ctcgatgttt ccggaaatcc cgtcggatga
    22681 atgaagatgg cccccgtact ccgctcgcga accacccacc tcccgattct cctcctgctg
    22741 ggactgctgt tcctgatccc ggcggcgccg ggacggggcg agtttcccaa gcccgtcggg
    22801 accgtcaacg atttcgccca ggtcatcggt ccggaggagt cgcgtcggat cgacgccctg
    22861 tcccgtgagg ttctggaaaa gacgggcgcc tccgtggtcg tggccacctt cgccaccctg
    22921 ggggaagcgg agatcaacga caccgtgaac ggcctgtacc aggcctgggg catcggcaag
    22981 cagggcgagg accggggcgt gctgatcttt ctcgccctca aggagcggaa aatccggatc
    23041 gagacgggct acggcgtgga gggcatcctg ccggacggcc gggtcggcga gatcctggac
    23101 cgccacgtcg tccccttcct ccgcgccggg gactacggga aggggctcct gaacgcggcg
    23161 ctcgccgtct cggaggtgat cgccggggat gccggcgtga ccctgacggg cagcccggcg
    23221 cccacgccac cgcccgtcac gcggcagcgg aaaggcatcg acgccctgac cctgctcttt
    23281 ctttttctga tcctgatccc ccttttggga acgcgccagg ggcgcgccat gctgccctgg
    23341 atcctgctgc ttttgatgag cgggagcggc ggccgcgggg ggggcggctt cggcggcttc
    23401 ggcggcgggg gatacggcgg cttcggcggc ggcggcagcg gcggcggcgg ggccggacgg
    23461 agtttttagg aaaggagcat cggatggcca gacgaccgaa acacccgcgg gagatcttcg
    23521 ccgagatcac ggcggactat cgccgcgtct tcggcgacgg actgatttcc ctgatcctct
    23581 acggcagcgc cgccgggccg gattacgttc cgagccgctc ggacctgaac tttctcgtcg
    23641 tcctcgacgg ggcggccatg gacggcctgg agcgggcctt cggcctcgtc gagcggtgga
    23701 aaaaacgcgg ggtggccacc ccgctgttca tgacgaaaac cgacatcgcc acctccctcg
    23761 acgtctatcc cgtcgaattc ctcaccatga gggaacacca tgttcgggtc gacggcgagg
    23821 acgttctgga ggggctcacg ttcgaccggg aacatctgcg cctgcaactg gagcgcgaac
    23881 tcaaggggaa gatcctgctc ctgcgggagc attacctgga aaccgagggc cgggtgaagg
    23941 ccgtccggga gttgatccgg gcatccgtaa cggcgatgat ctcgctgttt cacggcctcc
    24001 tgtttctgaa ggcgctcccg attccggccg ggcgaaggga gattgtccgc gacgcggcgg
    24061 aacactttgc gttcccggcc gacgttttcc tgacggcgct cgacatccgc gcgggaacgg
    24121 accgcctgtt agaccgggac gtgcaggcct ttttccgagg ctacctcggc gcgatccagg
    24181 cattgtgcac gcagatcgat cagtggcgga cctgacggag gaccccgcga cggcctgagc
    24241 gccgtcccgt cgccgccctg gacccaaacc gttatcaaca ccaaggagga accctatgtc
    24301 cacaggaaaa aaagcgctca tcgtcgtctt gatcgtcctt ctcgtcctcg gaatctctct
    24361 gtattccttc ttcaagggga actacaacac cttcgtccgc ctggacgagt cggtgaagag
    24421 ctcctgggcc caggtggaga accagctgca gcgccgctac gacctgatcc cgaacctggt
    24481 ggagacggtc aaggggtacg ccaagcagga gagggacgtg ctcgtggagg tgaccaacgc
    24541 ccgggcgcgg gtgggcggcg cggccaccgt tccggacaag atcgaggcca acaacgccct
    24601 gtcgggggcc ttgagccgcc tgctcgtcgt cgtggagcgg tatccggact tgaagtccaa
    24661 ccagaatttc ctgcacctcc aggacgagct ggcgggcacg gaaaaccgga tcgccgtgga
    24721 gcgcaagcgc tacaacgacg ccgtccggac ttacaacgtc gccatccgga ccttcccggc
    24781 caacctgctc gccggcctgt tcggcttcac gaaggcggcg ttcttcgagg ctccggcggc
    24841 ggcccaggcg gcgccgcagg tcaagtttta ggcgggtggg atgggtcggc ggatcgggcg
    24901 gaagcccggt ccgccggtga cgcgtcggcg agcgcctgaa gcagttcctg agccatgggc
    24961 ccttcccggc gccagaccca gcacgaatcg atcaggtact gctccatcag gcgggaggag
    25021 gtatcgctct ccgcgggctt ggcggcgtgg tggacccaga agccctgcac ggccgcgggc
    25081 gccatcgccg tgagcagggc gagcaggtgg gtgcatcccc gtcgtccccc gacggcctcc
    25141 cgggccttca gcgtgaatcc ggcgacgatg ttcatcccga cgaggcggtt taagcactga
    25201 gccgtttcca ggcagtcctc gcgcggcacg cccggcatct cgacctcgat ctccttgacg
    25261 gcgaggttga gcccttcgat cagcatccgg ataatcatgt gatgcaccgt gtggggcgac
    25321 cggatctctc cggaaacgcc gtaataggac cgcagccgct tctcgacgag ctcccccgtc
    25381 acgaggatgc cccggtcccc cgccggatag gtcaggatgt ccagggtccg gctgtgaacc
    25441 ttctcccccg tccgtttttc cagaagcccc atcgcccccc cttccctcgg atgatgaacg
    25501 atcccttgaa aatccggcca tggtcagcga cgccgcaaat ccgacgcttc gctttttcag
    25561 gcgcggttga tgaacgatcc ttcggaaaat cctgccccga ggctccgagg ctgtccgcgc
    25621 cggctcaggc cgtcgccgtc ctccagatcc ccttgcaggt gacgatccgg caggtctcgc
    25681 cggggcgggg cggaagcttg aaggtctcga tggtgtactc cggatcgaag ggcagtttga
    25741 ccccgaactc gccccccggt gtgaacggct gcgggaggat gggcgggatc tcgcgcccgg
    25801 agacggcctt gaacagtgtc tccgtggtcc ggtgcaccgc caactcctcg accgttttca
    25861 gggtcggggg catcagcacg atccggccgg cctcatgctc ggccagcgcc tgagccgggg
    25921 tcatccagac ggaagccgtc agttcggcct cgtcgtggac gggcctttgc cccgccggca
    25981 gccgcgcgag gaaaaagcgg gtgtcgaacc gcttcgactc gatctccggc gtcatccagt
    26041 gggcgtaggg caagagcaga tccaaggcga agcggagccc ttcccgggcg gccagatcct
    26101 tgagggtcat ctccccccgg tgcagaacct cccggtaggc ggcgaagcgc cgcgccgtct
    26161 ccggcgcatg gaaatcgacc gccgccccgg aggcgtccac ggcgagcaga acgccggcct
    26221 cctcgaacat ctcccggatg gccgtcaggt gaagtcccag ggccgtctcc ggcggcaggg
    26281 ccggctcctg gagccgttcc gccagcgcct ccggcgcgag cccatggagg tgcgcacgga
    26341 ggtcgggatg gccgtccccc tcgtcgagac gcccccccgg gaagacatag gcgccgccca
    26401 tgaaggcctg attgcggtgg cggcgcatga gaaagatctc gtacggaccg ccctcccggt
    26461 cccggaccag gatgacggtg gcggccggcc ggagttcagg aattgcaccc attttctttc
    26521 gctcctcgtt ctccgcgggg gcgtgcgaaa caccctccga caggatccct cgacgggaca
    26581 cgcgccccgg acgtcccggc cctacttggc cgtcatgccg ccgtccgcat ggatgatctg
    26641 gccggtcagg taggccgagg catccgacgc gagaaagacg gcgagcccct tcatatcgtc
    26701 catgtcgccg atccgatgca gcgggatggc gttcaggacc atctcgtagg cggcggaaaa
    26761 ctccggcttc tcgatgtagg ccatcatgtc cgtccggaaa aatcccgggg cgatcgcatt
    26821 gacccggatc ttgtaggggg cgagcttcac ggccaggttc atcgtgagca ggtcgatggc
    26881 cgccttgctc gaattgtacg ccaccgcggg atgggccatc tcctccgatc cgcgggaccc
    26941 catgatcgac gagacgttga tgatcgtccc cccgccctgc tccttcatca gacgggcgac
    27001 ctgctgcgtg atgatccaga cggcccgcac gttgaccttg aagatctgat cccaccgctc
    27061 cagcggatag tcgagggtag gcgcgcccca ggtgaccccg gcgttgttcg cgaggatgtc
    27121 gatacgcggg aatttttcac ggaccgcctg cagcatgtgc tggatctcct cgtccttggc
    27181 cagatcgcag gcgatgggca cggcctgcac accatacgcc tgggcgagtt cctgggcggt
    27241 cgcctcgaga ttcttcatct tgcgggacgt gatgatcacg tcggcgccgg cctcggcgaa
    27301 tcccgtggcg atgaacttgc cgatcccccg gccgccgccg gtaacgagcg cggtcttgcc
    27361 ctttaaattg tacatttcag aaagcttcat gaagacctcc ctgcctgaat gaatgtttcg
    27421 ccgtggatgg acccgatcct ccggccccgg gcgatggttc ccggtccgcg ccgtcccctg
    27481 gagcggtcat cgaacggacc ccgccgcctg aagcatatag tagcgatact ccgctcctgt
    27541 caagcccata atgccccgga ccggtttcgc gtcagcggcg atcccgcctt gacacggtcc
    27601 ggccttttca ttatacgttt gaccgaacat gatggaggaa cgaacgagat gagcgcctcc
    27661 ctggacaccg cccgtgcgcg ggcgaacacg gcccggtacc gtcccggaca gcgggcggga
    27721 cactacgaaa gcttctttca gcgggccaac cacccggacc ggccactggc cttctggatc
    27781 cgctacaccc tgttcagccc cgaggatcgc ccggacgcgg ccatgggcga gctctgggcc
    27841 gtctttttcg acggggagcg gaaccgccac tgggccgtca agcgggaggt cccgatgggc
    27901 gactgccggt tcgacgccga tgcgttctcc gtccgcgtcg gcgaggccgt gctggggccc
    27961 ggccatctca agggcgccgc ggcatcgggg ggccaccacc tctcctggca gctcactttc
    28021 gacggggccc agccccccct cttcctcctc ccgctcggcc tctacaatac gggatttccg
    28081 aaggccaaga gtctcgtcgg ccttcccctg gcgcggtacc ggggcttcat cgccgcggac
    28141 gaccggcgga tcgagatcga cgactggatc ggcagccaga accacaactg gggacggcgc
    28201 cacacggacc actacgcgtg ggggcaggtg gccggcttcg aaacgcaccc ggagagtttt
    28261 ctcgaggtgg cgaccgcgcg gatccgcatc gggcgcgggt ggagcccctt cctgacgacc
    28321 ctggtcctcc gccaccgcgg cgaggagatc cgcatgaaca gcctgccccg gatgctcagg
    28381 gcccgcgggt cgttctgcta cttcctctgg agctttcggt ccgaggaccg ccgacaccgc
    28441 gtggagggaa cgatctccgc gccccgggag gccttcgtcg gcctgaacta cctgaacccg
    28501 ccggggggcg acaagcattg cctgaacacc aagatcgccg cgtgcgaaat ccgggtgacc
    28561 gacaagcggg ccggcggcac ggcagcgccg gacgtcctct tcacccggca ccgggccgcc
    28621 ttcgaaatcc tcacggacga acgggaccac ggggtggcga tccaggcgta ggatcggaaa
    28681 ggatctccca tgcgtgaaaa ggtgcttcga acggacggac ccaccggcag cggacgagtc
    28741 gttgcctacc cgctggggga tggacgccgt ctgtttctca tccggaccta ctgcccggac
    28801 cggatcggcc ccggaccgac caacctctac ctcatcgagg acgaggccct catcctcgtc
    28861 gataccggcc tgcccacgga catggcgaag cagttcttct atttctggcg acggcagtcg
    28921 attccgcgcg ggatcgaggc gctgccggac gacttcagcg agcgggagct gacgagcgcc
    28981 ctcgcgctca ccggccaccg gatcgaggag atcgacgcga tcgtgctgac ccacgggcac
    29041 atggaccatt acctcctggg ccggaagatc gtggcgcggt ccaacgccag ggtggccgcc
    29101 catgtctcag acaccgcgat gatctgcaac ccctggggga tggcccgctt catggcggaa
    29161 cgatggccgg tcctgatggc gatgggcatg ccgccgccaa agcgtcacca gacgtccgag
    29221 tcgcggcggc aaacgcttcg gatcgatttg tccctccacg tcgacgagcc gatcgccgcg
    29281 gacgggcccc tgaccgtcgg cgacgccgtt ttcccccgcc tgacggtccg ccacttcgcc
    29341 ggtcattccc cggggggcat ctgcctactc gtcgactccg agaatgggtc cggccggcgg
    29401 atgctctgcg gcgacaccct cctgtacccc atcacgccgc accccgacga cctggtcgcc
    29461 tatctccgga ccttgaaggc catgaagacc ctggaggaca tctccctgac gctccccgcc
    29521 cacggcaagg cgatccgtcg cctgccggag cgccttgcgg cgatcgagca gcatcacgag
    29581 cgccgcctgc gactcaccta tgaggcgtgc cgccgacccc gcagcatctg gcagatcgcc
    29641 acgatgaccg gctatttcga cgtcctcgtg gaaccgacgc agttcaaccc cctggccggc
    29701 caggaggcct gggtgcatgc cgaactgctg tatctggtgg gcggactgcg ccggacgcac
    29761 atccgggagg gggttcatta tttcgagaac tcgggggagg ccttcgcggc aatctacgaa
    29821 cgcgtccggg agttgatcga cgacgagcag tccacccttc tcacgagacg atagggcctc
    29881 tcgatgctcc tcatccaccc tccggtcgtg aaaccggcgg aaccgccggc gggcatcgcc
    29941 cggctcgccg gagcgctccg gtcgaacggc gtcgcctgtc acctctggga cgccaacctg
    30001 gagggtttcc tgtccctgat ggcagcggca gagacaccgg gaaagccggc ggagagcgac
    30061 gatgcctgga cgcggcgggc gcggcggcac gccgaagcac acctctcctc cctccggcaa
    30121 tggccgctct atcgacagcc cgcgctctac cagcgcaccg tccgcgacgt caaccgcctc
    30181 ctccatgcgg cctcccccac cgccatccgc atcagcctga gcgattaccg ggatgccgac
    30241 ctctctccgc tcaggagccg cgatctcgcg agggcggcgg ctgagccgga gcgcaatccc
    30301 ttcttcccct ttttttcgga ccgcctgacc cggctcgtcg agacccggaa tcccggcacc
    30361 gtcggttttt ccctgaacta tctgagccag gcgctctgca ccttcgccat gatcggccac
    30421 ttgaggcgcc ggcatcccga gctcgagatc gtcgtgggcg gcagcctcgt cacctcctgg
    30481 gcgacccacc tgggaaacgg aagccccctg agggcgctcg cggatgactg gattgccgga
    30541 ccgggcgaag gacccctcct cgcccgccac ggcatcccgg cgggcgacgg cgcggactgg
    30601 ccgccggact acgacgacct gaccccggcg gcctacctgt ccccggggac ggtgctgccc
    30661 ttcagcgcct cgcgcggctg ttactggcgc cggtgctcct tctgcccgga gcaggcggaa
    30721 aaggccccct accgccccct caccgccgcg caggtccgga cgcaggtggc cgacctgcgc
    30781 gaccggacgc aacccgccct cctccatttt ttggacaacg ccctgagtcc ggccctactc
    30841 ggcgccctcg cggactatcc gccgaaggtc ccctggtacg gcttcacccg cttcacgccg
    30901 gagctggccc ggacggagtt ctgccgcgcc ctccggcggt ccggatgcgt catgctccaa
    30961 ctgggcctcg agtccggcga tcagggggtg ctcgacagcc tcgacaaggg catcgatctc
    31021 gcgaccgtct cgcgctgcct cacggccctc cggacggcgg ggatcgccgc ctacgtctac
    31081 ctcctgttcg gcgtccccac cgagaccccg gacgccgcgc gccgtaccct ggaattcacc
    31141 gtccgccacg cggaccggct cggctttctc aacctcgcca tcttcaacct ccccgtccag
    31201 gggccggaag ccgccccgct ggaaacggcg cccttctacg agggcgacct gtcgctgtat
    31261 cgccggttct cgcatccccg ggactggaac cgcgggaacg tcaggcgctt tctcgatcgg
    31321 gagttccgcc gccatccggc cctgacaccc atcctgcagc gcgatccccc ttttttcacc
    31381 tcgaaccacg cccccttttt cgtcatgacg ccgttcccct ccccgtcccc caaccctttc
    31441 ggaaaggagc ccgaaccatg aaaaagacca agatcatctg caccatcggc cccgcctgcg
    31501 acagcgccaa gatgatcgag aagctcatgc tgcacggcat ggactgcgcc cggctgaact
    31561 tctcgcacgg cctggcggag gagcacgtgg agaagatccg gctcttgcgc gaggtctcgg
    31621 agcggctggg aaagcccgtt cccatccttc aggacctcgc cggtccgaag atccgcatcg
    31681 gcctcctccc ggagtcgggt gtgcgcctcg ttcccggcca gaccttcatc ctgacgagcc
    31741 gggagatccc gggaagcgac gcggcggttt ccctctccta cccggacctg ccccgggagg
    31801 tccgcaaggg ggaccggctg ctcctggccg acggcctgat ggagctcgtc gtcgaggaca
    31861 ccgacgggac ggacatccgc tgttgcgtca tcacgggcgg cgtcctcacc tcgcacaagg
    31921 gcatcaacct cccgacgggg acgatccggg cgccggccct caccgccaag gaccgggagg
    31981 acctcgcggt cggcctggcc cacggcgtcg actacgtcgc actgtccttc gtccgcacgg
    32041 cggacgacat ccgggccgtc caggagctga tccggaaccg ggggctgacc accccggtca
    32101 tcgccaagat cgagaaacac gaggccctgg aggtgatcga ggagatcctc gacgtggcgg
    32161 acggcatcat ggtcgcccgg ggcgatctgg gggtggagat tcccctggcc gaggttcccc
    32221 tcatccagaa gaagctcatt catctggcca atgtccgggg aaagacggtg atcacggcga
    32281 cccagatgct gcgctccatg atcgactccc cccgcccgac ccgggcggag gccacggacg
    32341 tggccaacgc cgtcctggac gggacggacg cggtcatgct ctcggaagaa acagcgtcgg
    32401 gggactatcc ggtggaggcg gtggaattca tgctgaggct catcgagcgc gccgagacgg
    32461 gctatcccta tgcggacttt ctccaggcga agccggatcc cggggtgtcc gccgccgtgg
    32521 ccaaggcgtc ctgcgttctc gccgaccacc tcggggcgcg ctgcatcatc gcccacagcc
    32581 aatcgggtct gaccgcccgg gccctcgccc gcttccggcc tcggcagccg atcatcgccc
    32641 tgtctccgag cggggagacg gtccgccggc tcgccctggt ctggggctgt cagccgatcc
    32701 aggtccggga tttcaaggac acggacgacc tgatcgaaaa ggccgcgcag gccgccctcg
    32761 ccgccggcga actcgccccg ggagagctcg cggtgattac gctggggcac cccctctgga
    32821 cgaccggatc gaccaacatg atccgggtga aagagctctg acgacggcgt cggcctcagc
    32881 cgaaaacctg gtggttcaga agtttccagg cttcactgcg gacgaattcc gtcacccggt
    32941 cgccctcgaa gcggaggatc ccctcgcggc cgagcttgcg gagcacctcc cgaatgtccc
    33001 cggggtcctt ccccgtatag cggaccagct ccgtgaccgg gtcctgcccc tgagcgaggg
    33061 gccattcctt gagaaagtac aggatcgcca tcagttcgat ccaggcctcg gtcagttcct
    33121 cgaggacctc gttcgtatgc ttgatccggc gggaagacgc ccgcagcatt aacagcgaga
    33181 cgtcgtcgct ctcccggagc atccggtgaa aggtctcgct gtcgatcacg gcgagacagc
    33241 tgtcggacgc cgcctcggcg gaggcggtcc ggggggcatc gatcagggtg gacatttccc
    33301 cgaaatactc ccccggcccc acgtcggtga ggaccttctt gatctgcccg gcctgcttct
    33361 gcaccaggat ccgcccttgc aggatgtaat acatctcgct cccggtgtcg ccctcccgaa
    33421 agacgaagct ccctttcgga tacacccggc cgaaccggcg caggaggcgt tcatcgaccg
    33481 acgaccgggc ctttcccggc gtgagcaggg ccttttccac tagcaggatg tcccgccgct
    33541 ggtaggacgc gatcagctcg gcgcagaaca gaaggatcag ggccacgtaa aagacccaga
    33601 tcacgaggat gaccaccgct tcgagcccac cgaagatcac gtggtaacgc gtgtagttgg
    33661 cgatatacca ggcaaagaag tgcttcgcca cctccatcag ggccgaaaag agcaggctcg
    33721 ccatcagggc cgctccccag gagacccgtg cggtgggcgt cgccttgtag acgacggtga
    33781 agaaggcgac cgtgatggcg tagggcagaa tgtaccggaa caggacgccg tgaagcaggg
    33841 agagataggc aaagtgctcc atcagcaggg gctgccgcgc caggagactc ccaacatacg
    33901 ccaggcccac gctcgccccg atgatgatcc agcccaggag gatcagggcg acggccagaa
    33961 gcttcgacag gacataactg cgccggacgt gtgaacggaa gatgatgttc atcaccgttt
    34021 cgatggcgtt gaagatcatg gcggaaaacc agacgaggct gatgatcccg acccatccca
    34081 ggatctggcg tttctggtcg atccggccga gctgttcgag aaggctctgg gacaggtagg
    34141 ggctcacccc ctgcgccccc tgaacgagct gctgctggat ctgcggattc gagctgaaga
    34201 cgaggctcgc catcagaatg gtgaggatga acagcgggat gatggagagg atggcgtaca
    34261 gggcgatcgc cgccgcctga ttggtgtcgc cgttgatctg ataattccgg agtgtgtccc
    34321 gcaggacgtc ccgtgcggtc tcgaacccca ggcggaggcg ttttatttcc ggcttttcgt
    34381 gcttttgcat acgctcctgg agaccgtcga aaaccgcgat cccgccggca gggcgtctcc
    34441 ggccttcttc tgcatcgcgc gatcccggaa atccggggcc gattttttaa actataccca
    34501 aaccggtcct gtcaagcaaa aagagccgca attcctgttg caaaccggag gtccttgggc
    34561 tagtttggcg tcatgcgcat ccggcttccg atccttcccc tgtgcctcgc caccctgctg
    34621 gtttgttcgg ctgcctgcgg gcagcgcccc tccgaaccgg gccccgccgg accgggcgcg
    34681 gaatcacccc cggcccacgg cgacgtcctg gtggaagggg ccatcggcga cgcctccaac
    34741 ctgatccccc tgctggcctc cgacagcgcc tcgcacgaca tcgcgggcct ggtctacaac
    34801 ggcctcgtca aatacgacaa ggacatcaac atcgtgggcg acctggccga atcctgggag
    34861 atcggccggg aggggctcga aatcaccttt cacctccgca agggggtccg ctggcacgat
    34921 ggccgtccct tcacggcgga ggacgtcctc tacacctatc aggtcaccat cgatccaaaa
    34981 acacccacgg cctacgccgg cgatttcctg cgggtgaaaa aggccgaggt cctcgacccc
    35041 tataccttcc gtgtccgcta tgaccgcccc ttcgccccgg ccctgatgag ctggggaagc
    35101 gccgtcctgc ctaaacacct cctggcgggc cgggacatca ctcagagccc gctcgcccga
    35161 catccagtcg gcacgggccc ctatcgcttc aaggaatggg tgacgggcca gaaaatcgtt
    35221 cttgccgcca atccggacta cttcgaaggg cgcccttaca tcgacggcta cgtcctcagg
    35281 atcatccccg acacggccac gatgtttctg gagctgcggg cgggcgggat cgaccgcatg
    35341 ggcttgagcc ccctgcaata cacccgccag actgagaccc ccctcttccg ccgggatttc
    35401 aacaaatacc gctacctgtc cttttcctac acctacctcg ggtacaacct gaagaaccct
    35461 ctgttcgccg atccccgggt ccgcagggcc atcgcccacg ccgtcaacaa ggacgagatc
    35521 atccggggcg tgcttctggg gctgggccaa ccggcaacgg gaccactgaa accctggacc
    35581 tgggcctaca acccgcgggt ggagcggtat ccctacgatc cggggaaggc ccgggcgctc
    35641 ctcgccgagg cggggtggcg ggaccggaac ggcgacgggg tgctggaccg ggacggccgc
    35701 cccttccgct tcgaaatcat cacgaaccag ggcaacgagg tccggcaaaa gacggcggag
    35761 atcctccagc ggcgcctggg cgaggtcggg atcgaggtga agatccgcgt cctggagtgg
    35821 gccgccttcg tcaacgactt catcaacaag cgcaagttcg aggcgacgat tctgggctgg
    35881 acgatcccga tggatcccga tctctacgat gtctggcact cgtcgaaaac cggcccggag
    35941 gaactcaact tcatttcgta ccgcaatccc gaggtggacg ccctgctcga acaggggcga
    36001 agcacgtttg acctgaagct gcggaagcgc tgctacgacc ggattcagga ggtcctcgcc
    36061 gacgaacagc cctacctctt cctctactgt cccgacgccc tgcccatcat ccaggcccgc
    36121 ttccggggga tccggccggc ccccctcggc atcggctaca atttcatccg gtggtacgtg
    36181 ccgcacgggg aacagcggct ggtgatgacg ccgtaagccg gcggaaaccg tcaggcctcc
    36241 cgtttcagac ggtccagcag atactgccgg aaggcccgat aatcggcgcg cagggaaagc
    36301 gcttcccccg tgcgttgatc gaggtcccgc atgccggcgg gcgggaccgg atcgatcccg
    36361 agcagggcgc ggatctcctc cgggaatttc gccggatggg ccgtttcgag cacaaggcac
    36421 aggggggagt tttcctcacc gcgcacctcg aggaacagct cgagtccccg ccagcccacg
    36481 gccccgtggg gctccagcag gattccgtac tgatcgtaca cccgccggat cgtccggcgc
    36541 gtctcatcgt cggagacgct gacggaaaag atgcgtcggc gcatctcggc cacgtccggc
    36601 agacggtgga cccggccgtt ccggtccacc gtccccccgt atagatcgaa aaagcgcgcc
    36661 agattactcg gatggcccac attcatggcg ttcgacaggc aggcccggga cggctggacc
    36721 ttttcgtacg cccccgtggc caggaaattc gggaattcgt cgttctcgtt cgtcggcatg
    36781 acaagcgttg ccacgggcag gcccatccgc cgggcgtatt cgcagcccag ggcgtcgccg
    36841 aagtttccgg agggcacgga gacgacgacg gcctcgccgt tctcggcgag ttgggcgtag
    36901 gaccagatgt aatagacgat ctggggcagg atccgtccga aattgatcga attggccgac
    36961 gtgaggttga aggccgcgag atccggatcg gcaaaggcct gcttgaccag ggtctggcag
    37021 tcgtcgaact tcccgtcgat ggacaaggcc tggatgtttg cgccgatcgt gtccaattgc
    37081 cgcttctggc gaccgctgac ctccgcccgg gggtagagga tcgtgacgtc gatgccgggg
    37141 acgcccttga aggcctcacc cacggcgctc cccgtatcgc ccgaggtggc gacgaggacg
    37201 ttcaggcggc gtccggcctc gcggaaatgg cccatcaggc gggccatcag gcgggcggca
    37261 aaatccttga aggaggccgt gggcccctgg tcgaggcgca gcaagtattt cctcccagtg
    37321 acgcgctcca ggggaacact gaagggatag gcgtcttcga cgaggcgctt caggtcgtcc
    37381 cgggcgatct cgtctttgag aaaggcctcc gccacgaaga aggccgcctc gggataggga
    37441 cggcccttca gggcgaggat ctccgcgagc gacagggtcg ggatggcggc aggcatgaaa
    37501 agcccctcgt cgggggcctg ccccatgagc agggcctccc ggaacgcaac ctgtcccttg
    37561 aaaggggtga tcccggggac cccgtcgagt tcgcggttcg tgctgtaata tcgtatggtt
    37621 gctgaggaca tgccgaattc cattccttcc cgcggcgttg ccgccgcctc ccggcgcccg
    37681 cggtccctcc ggatcggggc cggaccggcc gttcttttta ccaaatccgc cgtccggaat
    37741 gcaaggcccg atcttcgccg ggaggccgga agcggggcgg cgcccccttt cttctttccg
    37801 cggcgcggcg tttgacgctg cgatccggat gcagaatcgc ctttatgaac gggggccgat
    37861 gtgatagaga agaacgccag gatcaaaccg tcgccctccg caaacccacc gggtcgggac
    37921 atccggccga tatcgggtgg gcggagggga gggcggggcg ccgattccgg cccgacgatc
    37981 gtcccagcga ggagcgccca tgagcgaccc cgagaaggcc gtccgactga ccgaaaccgt
    38041 ccacggggcg gggtgagcct gtaaaatcgg tccgggcgac ctgcacgaag tcttgaaaga
    38101 ccttcccttc atcgccgacc ccaacctcat cgtggggatg gagcacggcg aggacgccgg
    38161 ggtctatcgt ctgcggaacg acctggccat catccagacg gtggatttct tcaccccgat
    38221 cgtggacgat ccttatctgt tcggacagat cgccgcgacg aacgccctga gcgacgtgta
    38281 cgccatgggc gggcgaccgc tcacggcgct gaacatcgtc tgcttcccaa ttcacaagct
    38341 cgacacggcc attctgaagg cgatcctccg cggcggcctg gacaagctgc gggaggcgga
    38401 cgtgctcctc gtcggcggcc acagcgtgga ggacaacgaa accaagtacg gcctggccgt
    38461 caccggcgtg gtccacccgg agaaggtcct cctcaaccgg ggcgcccggc tcggcgaccg
    38521 tctgatcctg accaagcccc tgggcaccgg catcgtcggc accgcgatca agggcggcgt
    38581 cgccgcgccg gaactcgtgg accacgccct ccggacgatg accgccctga accgtcgggc
    38641 ggcggaactg ctgtccgacg cgacggaggt ccacgcggtc acggacgtga cgggattcgg
    38701 cctgctgggg cacgccctgg aaatggtcga ggacagcaac gtgggcctgg tcatcgacgc
    38761 cgcgcgggtc ccggtcttcc cgggcctcag agacctcgtc gagagcggca tcgtccccgc
    38821 cggactccat cgcaaccgcc agtaccgcga acgccagatc gaggcggcgc cctcctgccc
    38881 ggcgtggctg ctggacgtcc tttttgatcc ccagacgtcg gggggccttc tggtcgcgct
    38941 tccggaaaag gatgcgtcgg cctacctcga gaacctgcac gcgaacggcc ttcgtcaggc
    39001 ggccgtcgtg ggccgggtcg aggcttcgcc cccggggcgg gtcctgatcc gctgaggcgc
    39061 aaaccgccgc cccgcggacg gatcaggccg cgcgggagcg gcggggccaa agtttccggc
    39121 cgtgccgccc cggcaccgcc ggcttgaaac gagcgcggat ttgtgttaac cggggcgatc
    39181 ggcgacaccg tcgaaaggac cgctgctgaa agaggcccca tgaaacgtgc agagtcaatc
    39241 ctttccaagg aaatcacgcg gaacaacctc agccggctga catcgctcgc ccgcaagaac
    39301 gacagcctcg ccatcctcat gtacggggcg cccgatccgg atgccgtggc ctcggccatg
    39361 gccctcaaag agatcctcgg caagaccgtc ggcctcgcga aatgcacctt cgtctcgacc
    39421 gatcccgtcg cgcggcagca gaacgtggag ctcatccggg agatgaagat ctcgatcgag
    39481 ctgctggaca aggtccaact cgccgattac cgcctcatcg ccctagtgga cgcccagccc
    39541 cccttcttcg gaaaggccct ggagaacatc accccgcaga tcgttctgga ccatcacccc
    39601 cgcgacggcg agtggcgcgc cgatctggag gacatccgcc cccgctacgg ggccctgtcc
    39661 acgatcctga tggaatacct gctggcggcc cagatcaaga tctcccgccg gctctacacg
    39721 gcgctcctct acgggatcaa gaccgacacg aacagtttcg agcgggacgc catcctcgag
    39781 gacgtcagcg cctattacgt caccttctcc cgggcaaacc ggcaactcat ccgccgcatc
    39841 gaactcaacc agatccccga tcgcttcatc aagtacttcg accacgccta ccattaccgc
    39901 cgccgctacc gggaccggat catctgctac ctgggacgcg tggagaatac ggacgtctgc
    39961 gtgcaggtgg ccgatttctt cctcaacctg atcaacatct actacgtcgt catcgcgggg
    40021 atgacgaagg atcgcctgat catcgtcttc cggggggacg gctatcgcca ggactgcggc
    40081 gccatcgccc agaaggcctt cggcgcctac gggagcgccg ggggacacaa gagcgcggcc
    40141 cgcgtcgaga tcgccctgga ccccctgcgg gacctgctca agaacgacct gacccaggag
    40201 ggcgtagacc acttcctcgt gcagcatctc cggggcaagc gtaagaaaaa ggaaccatga
    40261 acgatttcgg cctttcgaag gaaacgagcg tcggcaccta cctggacccg atgaacggct
    40321 tccgtcagaa ggcgtccgtt ttcgagatcc gccgggacga gatcggcggc gtcacgagcc
    40381 ggattctccc ctatcggttc aagatgccgg agagacccga cctcagcggc tacctggaga
    40441 aatccccccc cgccatctgc cccttctgcc ccgatctgat cgacccgctg acgccccgct
    40501 ttacggcgga catctgtccg gaggggagat tccggcgcgg gcgcgcgacc ctcttcccca
    40561 acgccttccc ctatgaccgg cacaacgccg tgatcaccct ctcggacgaa catttcgtcg
    40621 gcctggacgc gttccgcccc gaatcgatgc aagatggctt ccacctcgcc tgggactact
    40681 tcgagcggat ggtaaaaatc gagccgaccc tccggttcgc ctccctcaac tggaactaca
    40741 tgccgcccgc gggcggcggg ctcatccacc cccatcttca gacgatcctg ggggagaaac
    40801 cgacgcgatt cgcggcacgc ctcctggacg gcgccggtgc ctaccgggag cgaatggggc
    40861 gggacctctg gacggatttc ctggcgcggg aaaaaaccct cggggagcgc cacatcgccc
    40921 aaacgggacc cgtgacctgg ctcgccagct ttgcgccgaa aggcatgggg ggagaggttg
    40981 cgttttactt cggggagagg acggccttgc gccagatctc agcggcggat ctgttggggt
    41041 ttctggacgg attggcctgc gttctcggat atctgcatgc aaacaacttt atcagtttca
    41101 acctggctct ctacgggtcc tgggaggaaa atcgcgactt cacggtgcag gggcggatca
    41161 gtccgcgttt ctccattccg cccctgggga cgagcgacat caactacatc gaaaaatacc
    41221 acgacgaggt catctgcccc gtcgttcccg aggacctctg ccgggagatc ctccccttct
    41281 tcgcggccgt gattccctga ctcattcctc cgaaccgccc ttgggggccg cttcggcctt
    41341 gggcgtttca gccggcgcgg ccgactccga cgcttccttc ttttcggtgt ggggaacctt
    41401 ctttccgccg taatccgtca cgtaccagcc ggaccccttc agatggaacg atgacaggga
    41461 catcagcttg ttcaccggcc ccttgcaaaa ccggcaggac ttgaccgccg gatcgctgat
    41521 atgctgaaat acctcgaatt cctttccgca tttcttgcat ttgtactcgt aaatgggcat
    41581 cgataaacct ccaacagggt taatgatcga gatcgccgat ccggtagcgg tcgctgaagc
    41641 agtccagcac cagccccagt tcgggattcg ccgccggctg aagcatgcgc cgggtgatct
    41701 gatggaccgt ttcctcctcc tcatccttga cctcgcgcgg ggcgtcgaaa gggatcaccc
    41761 cctcgtcgaa ccggatgacg tgcaccagtt cgtgggtcgc gatataaagg agcagggggc
    41821 tcaggcgaat gaaggagtgg gcccgcttca cggcatcgag gatgcggtcg tcctggaggc
    41881 agatccggta gaaatgccgg tccccggcgc cgacgtcccc ggtttccttg ccgtattcat
    41941 acttgcacag atgggcgaac gcctgaccct ggacttcatg ctccttcaga taggccttcg
    42001 tcctgacgtc ataccgatgg gccttcaacg ccccgggacc gagacgaaaa tagcccgaag
    42061 cgagcgcctc ggcgcggaca aacgcctgat ccgcaaggga tatctgttct gggctgaaat
    42121 acatggctca cgacgtctcc ccggaaaacc ttcaccaccg gcgtcggtta taatataacc
    42181 atttccccct gcctgtcaac cggtttccag gacccgggac gaggggggat tgcgacgttc
    42241 agggctcggg ccggggggga gccgtatcgg tgagaaacgg gaggggcttc gccatgcggt
    42301 acaccaaggc ctgacaggag accagcaggc ggccgtcttc gtcctccacc cggatctcgt
    42361 agacagcggt gcggttcgac cggttcagtt ccttcgcctc cgcggtcagc agggcgcctg
    42421 gacgcgggga agcgaggtag gcgacgttca tgctcagggc gaccgccatc gtgccgtggg
    42481 agttggaggc cgtctcgaag gcctcgtcga cgagggcgaa cagggccccg ccgtgggcca
    42541 tgccgaagag attctccatg tccggggtga agcgcatctc gacgcgggaa tggccgtcgt
    42601 ccaatgccag cagcttcaaa ccgaattttt gcgcaaaagg ctcctccgcc acccggcgga
    42661 agatcgcctc acggaccctg tcgtccacaa catcgccctc aaacgccctc agggaaggga
    42721 aacctcgatc tcgcatccgg ccgtaaagaa ttcggccaca tcctccgccc ccagggtcag
    42781 ccccgaacgg acggcgcgct cgaaagacga ccagcggctc cggtactggt agatcccgcc
    42841 ggccagggtc ttcatgttga tgtggctcat cggataggga ctggcaacga tcagaaacgt
    42901 ctcccggctg ccgggtgggg gtgaaaggac gaaccggtcc ccctcccccg gcgcctggta
    42961 gcggacaccc ttccggaaag acgacaactg ccggtagttg ttgggaagga tctgttcgat
    43021 ggcgccgctg gccgtgaccc gcacgagctt cgcgtagaaa tcccggttcc cctcgaaata
    43081 aaccgtcacc ggctctccgg cgcggtaccg cttcttgtcc gtccagagcc ggacgttcag
    43141 cagacccgcc cggtcgatgg ccggctgccg cgatgtcgcg tcgctcccgg acgccgggcg
    43201 gaaggtgtaa tccacctcca tgtcgatccg cacgcggggg cgtccggttt cgtccggccc
    43261 cagggtgcgc cgatcgagga tgcggaccga cccctccggc gccgtccgga ggatttcgta
    43321 gccgaacccg acgccccgga acttccgctc gaggtactgc tgcgcccgct tcagggccgc
    43381 tttccgggcc tcacccacgg cccggttcag ccgctcggtc tccggaacgt tcttcccccg
    43441 gtcgccggac acctcggcgg actgaatcag ggaataccgt tccgcaagct gcgggccccg
    43501 ggacggcgcg gcgcgcttcg ccgcctccgc gggagatccg aaggtcatcg ccaggaaaag
    43561 gcttaaaaaa caggcccaaa cacacagacc agccggctgc cgtttcgaca tgattcccct
    43621 ccttttccgc cgggtccgtc ccggccgttc ggtgaccccc ctccggtcag gatttcggcc
    43681 cgctatttcg gaatgggcac actgctcagg agatacttca ggtcgtcgtc ctcgaagcgg
    43741 atcgcaaagc ccgcgaaggg agagctgttg gaccgcttgc tgatgaagtc gtcccagcct
    43801 gcggtcagat agatgtgctt gaacacctgg aactccatcc cggccttcag atgggccctg
    43861 tcatcgtcat cggaattgaa gtcgaaggcc tcgaaactga gcttcaggcg gtcgttcagc
    43921 atcagataat ccagccccac gccgcccgta gactccagca cgccgccccg caggacgatg
    43981 ttccggaacc gcttggccac ctgggcgttg aagagaagcc cgtccttttc ccattcctcg
    44041 gtgcgcgtca tcacgccgtt cgtgatcctc tccttgacgg tccggcgccc gcggggatcg
    44101 gccacaaccc ccacgaggta gtaacggtcc tccttcggct ggatccggat gtcgacgtag
    44161 ctcttgccgt cgctcttgtc gaacagatac tcgccccggt agttcagata ggtcctgaac
    44221 tgctcggatt tgctgacgta gcggttgatt cccttaatgg tctcgtccag gttcctggcg
    44281 gtctgctcgt cgttgatgag cttgccgatg gttcccttgc cctggttgat cttgtccgaa
    44341 acctcctgca gggacgtcat ggtctggctc aacttctggg ccgtttcctg attcttgacc
    44401 aggtgtccga ggctcccctc gccgcggttg atgctacccg tgatctcgtt caactgcgcg
    44461 aaggtctgct ccatctgctt ggaggcgtcc ttgaggttgt ccatgacatc ggtgattttc
    44521 gcgtcgttcg tgacgacgac ccggttgagg ttgtccgtca gctgccgggc gttgtcgagt
    44581 gtgaaccgga gggcctcttc gttgttgacg acgagggcgt tcaggttctt tgtcaggaga
    44641 cgcgcgttct ccacgatctc ctccaaggag gcttcgcccc gctccccgcc catggccttg
    44701 ttcagggacg aggtcacgcc gcgaatgtca tccgcgatca gggcgatctg gtggaggagt
    44761 cggtcgatgt cggcctggct ctcgacctgt tggatctggt ccccctcctt caggaaggcc
    44821 tgcgtattgg tgcccggagc cagttcgata tacttgtccc ccagaacccc atgggtcttg
    44881 atcgtagccg cggcgtcgtt tcccagcttg acgttcggcc ggatgcgcag cgtaaccagc
    44941 gcctggccgt cctgaagctg gatttcctcc acccggccga tctccacgcc ggcgatctgc
    45001 accgacgcgt cccgatccag cccggcgacg ctcggaaaga ccgcgcgcac cgtatagccg
    45061 ttcttcaggc cgaacccctg ctgccccacc cggaaggaca tgtatcccag gataatgagc
    45121 ccgaccagca cgaacaggcc gactttcgct tcagaactga tgttcaccat tcctgcctcc
    45181 ggcattagac gattttgatg gggccctcga tgctccccga cagaaattga ccgatgacgg
    45241 ggttggcgga ggtccggatc tcgtccgggc tcccatgggc gatgatcttt ccctcgtaga
    45301 gcatggcgat ccggtgtcct atctcgaaaa tggactggat gtcgtggctg atcaccacac
    45361 aggtcagctg gaagcgctgc tgggtgtcga tgatcagacg gttgatcgcc tccgtcatga
    45421 ccggatcgag cccggtcgtc ggttcatcga acaggacgat ctccggattc aacgccacgg
    45481 cgcgggccaa gccgacccgc ttgcgcatcc cgccgctcaa ttccgcgggc atcttcgcct
    45541 cgacgcccgt caggccgacg gcctgaaggc gctcggagac gatccgccgg atttcgtcct
    45601 ccgggtaagg cgtgtgctcg cgcaggggga aggcgacgtt ttcccccacc gtcatggaat
    45661 cgaacagggc cgcctcctgg aagagcatcc cgaacttctt ccggatctcg ttcaggtccc
    45721 ggtcgttgag ccgagtgatg tccacgtcgt cgatgaaaat tcgtccgcgg tccggcttca
    45781 tcagtccgat gatgtgcttg aggaggacgc ttttgcctcc cccgctgcgg ccgatgatca
    45841 ccgtcgtctt tccgctctcg atctccagat cgacgccgtc cagaaccgtc tgccggccga
    45901 acgccttatg aacctcttcc agacgaatca aattccctca cccttttttt cttcgccctt
    45961 cggcttttac cctcgcctag aacatgatcg ccgtcagaaa atagtcgctg atcagaatgg
    46021 tgatggcggc cagtacgacg gcctccgtcg tggcccgccc gaccccttcg gcgcccccgc
    46081 gcgcattgaa gcccttgtag cagccgatga gcgacaggat cagtccgaaa caggccgcct
    46141 tgacgagccc gttgtagata tcccccagat ccaccagttt ggtaatattc ttgataaaga
    46201 tgccggagtt gatgtcgaga aggtgaaccc cgacgaaata cccccccacg atcccgcaga
    46261 aatccgaaac gacggtcagg aggggcacca tgaacacgcc cgccagaacg cgcggaacga
    46321 ttaggtgttt gatggggttc gccgccatga cggagagggc gtcgatctgc tccgtgaccc
    46381 gcatcgttcc cagttcggcc gccatggcgg accccgcccg cgcggtcacc atcagggcgg
    46441 tcaggaccgg ccccagttcc cgggtcatgc cgagggcgac ggtcgacccg accagcgcct
    46501 cggcgccgaa catccggaaa ccgtgatacc cctgcagcgc catgaccatc ccggtaaagg
    46561 tcccggtcag aaccacgacg aagatcgact tgacgcccac gaattccatc tgcttgaaga
    46621 tgttgcgcgc cttgaggggc ggccggaaca tccaggcccc gacggacagg aacatgagca
    46681 ggatccgccc catttcctcc acgtaggaca gcacggcctt gcccagtcca tccagggcgg
    46741 cggaaatggc gttcatctac aggggccccc ttttgttggc gtgcacctcg acggcggcgg
    46801 cgatgatggc ctcgatcaga cccgcctgcg tgatccccga ggccgccgcc tggtgaaaga
    46861 ggacggtgga gggcgtcatg ccgggaaggg tgttcggttc gaggaccacc acccgcccat
    46921 ccgccaggac aaacatgtcg atgcgggcat aacccctgag gtccagggcg cgaaaggtcc
    46981 gaacggcgac gtcctggatc cgggtgagcg tctcttcggg tagccgggcg ggggtcttgt
    47041 tttccccctg cccgtagaga aacttgtctt cgagggacag gacctcggcg gtcgcgatcg
    47101 tctccgacgg cgtcagcgcc cgcggggcgt ccgttccgat cacgccgcag gtgacctcca
    47161 tccccgcgag gcactcctcc accagggcct gccggtccca ccggaaggcc tcggtgaggg
    47221 cggccgccag cgcctcccgc gtcgaaacct tttccacccc cgtgctgcat ccctcccggg
    47281 ctggtttgac gacgcacggg tagccgatcc ggccctcgat gtccgccagg atttcctcct
    47341 gccgccccgc ggaccagtcg gcgatctcca ggggggccgt ccgggggacg tcgatgcccc
    47401 cgagggcgag aaccttgcgg ctggcgtact tgtccatgcc cagggccgag gccagcacgc
    47461 cggatcccgt gtaggggatc ttcaagagtt ccaggagtcc ctgcatgcac ccgtcctcgc
    47521 cgtacttgcc gtgcagaccc aggcagacga cgtccacccg cgccttcagc gcctcgtacg
    47581 gaaccggcgt cgcttccgtt tccaggtcct cctcgatgtc ccgggtgctg ttgcgcatga
    47641 ggagcttgag cgggatctcc cagaggcggg cccggctgtc catgaagatg ggaaccggct
    47701 cataggcgcc gcggtcgatc ttgctgaaga tgttccggcc gctttcgagc gagacctcct
    47761 tctccgagga gatccccccc atgatgacac cgaccttcaa ttttcctttt cccgtgcttt
    47821 ccatgccttg aaatcttcgt cccctttaga atccgagcgt caggcccgca tatggacccg
    47881 tatagaacac atccatcatg tagttgttcc ggtccacctt gagctggacc cgtttgcagc
    47941 cggcgtggag gtcatcatac cctcctcttt cgggattttt tcgatttctc ccggtccctc
    48001 cgggaggccg caacgggatg ctctggcgac gttccgcggg gtcccgggcg gaaccggatc
    48061 acgatctctt tctatatgat cgttcggtgc gcttgtaaag acctatagcg ggccgaactt
    48121 ttcctgatgg atcctccggg cgttctcgat caggcgcgag atgatcatcg acgggagcat
    48181 cccggaagac gcggcctgct cgaagaagaa ggaagacggg gccatgcccg aagaggaatt
    48241 gggatccgtg atcaggatcc ggccgtcggc cagataaaac ccgtcgatcc ggccgtagga
    48301 acgaaacccc agggcccgga aggcgcgcag cacctcggtc ctgatccgct ccaccctgtc
    48361 cgcggggatg ttcttgggcg gcgtgaactt ccggcagcgg ccgggcatgt acttgtcgtc
    48421 ataggtaaaa tattcgctct ggggcatgat ttccgtcacc tcgagcggcc gggccacgcc
    48481 atcctcctcc agaacgatgc tggagaattc gatgccgtcc agaaactctt ccaccaggac
    48541 ggcccggtcc cattccaggg ccgcctcgat tcccttgtcc agatcgttcc cgtccctgat
    48601 cacggcgacg ccgacgctcg atccctcccg gatcggcttg gtgaaaaacg ggaagggaaa
    48661 gtgttcgcat acccgtttct tgacggcggt ccggtccctc cgccattcct cctcgcggat
    48721 gaggagactg tcgggaacgt cgatgccggc ggcccgcagg attttctgct ggaggtgctt
    48781 gtccatcccc agggccgagg ccaggacgcc cggtccggtg tagggaatcc tcagaagttc
    48841 caggagcccc tggatgcacc cgtcgtcgcc gtacttgccg tgtagggaaa tgaaggcaaa
    48901 atcgatctcc cgggcgagcc cctcgtacgg gatgcgtcgg gcctcggttt cgaggcgctc
    48961 cacgatgtcc gtcgtggtgt tctgcgaaac ga
//
LOCUS       DAOOLJ010000014        45558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_575_length_45558_cov_15.119465, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000014 DAOOLJ010000000
VERSION     DAOOLJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 45558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45558
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_575_length_45558_cov_15.119465"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLW16_04340"
     CDS             <1..482
                     /locus_tag="PLW16_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPB70167.1"
                     /translation="FYDTDDLVRERTGMSVRRIVAEQGWASFRSAERAVIADLAFLKG
                     AVVALGGGVVLDPENVAAVKANGRVIWLVADARTIVARMGEDPVSATQRPSLRGVDRL
                     KETVQILAERTPVYRAAADFTVDTTGKTVDEIAAEIGGFLARSPDATPPDGTDPCGS"
     gene            613..1668
                     /gene="aroC"
                     /locus_tag="PLW16_04345"
     CDS             613..1668
                     /gene="aroC"
                     /locus_tag="PLW16_04345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPB70168.1"
                     /translation="MSGNTTGIVFRVTTWGESHGEAVGAVVDGCPPGIALAAEAIQRE
                     LDRRRPGSDALASPRREEDWVEILSGVFEGRTTGTPIALTIRNSDAASAAYDDLRDVF
                     RPGHGDFTYWKKYGLRDHRGGGRASGRETAARVAAGAVARRVIAPAGITVAAFTRALG
                     GIVAKSDPPGDADRNRLRCPDAAAAERMERRLAGAKRQGDTLGGVAEICIHGCPPGLG
                     EPVFDKLDADLARALMGIGTVKGVEIGAGFAAAAMKGSRCNDPIGPAGFASNHAGGVL
                     AGISNGEAILLRAACKPIPSIGRAQETVDVRGNPATLAVRGRHDVCVIPRILPVCEAM
                     VCLVLADHLLRQKAIRP"
     gene            1665..2435
                     /locus_tag="PLW16_04350"
     CDS             1665..2435
                     /locus_tag="PLW16_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPB70169.1"
                     /translation="MNPFEIGIVGGTGGIGRWFAAFFESEGYPVHVVGRRTDLTFRDL
                     AERCRVVIVAVPIGVTQEVIHRVGPLLDPGALLMDLTSFKAAPVRAMLDASAAEVIGC
                     HPLFGPDVPSLEGQNVAICPARGERWLPWLRDRLGKNGARIVETTPERHDRMMALVQA
                     LNHLDTIALGLTLREAGVSPADLEPFTTPIFRMKAAIVEKVFGASPRLYAELIAGNPH
                     TDELLAIYETQLARLKDLLARKDIDGFAAALNPGKGSP"
     gene            2432..3610
                     /locus_tag="PLW16_04355"
     CDS             2432..3610
                     /locus_tag="PLW16_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB70170.1"
                     /translation="MKGLALFLLAFLILYGGLHAYAFLKVRAAFPIPPPLHVGLILLL
                     GLLFLAPLLIRYSEGAGFETLARGLSHVGYGWMGFLFLFCAAALCVDLFRVLISAGGV
                     AFKQTLAPLLPSPRLVFLIPLIVAAVAMAYGYGEALSIRTERLVIRTAKLPAGVDRLR
                     IVQISDIHIGLIVREDRLDKIVAAVQAAGPDIVVSTGDLVDSQLDHIRTAMERLRALP
                     ARYGKYAITGNHEFYAGLDDALAFTEGAGFTMLRDEGVLAAGAVQIVGMDDVPGHGLA
                     SPTVSPEQTLLRGLAPDRFTLLLKHKPVVEKESLGRFDLQLSGHTHRGQLFPFSLITR
                     LFFPFHAGAYDLPGGGILYVSRGTGTWGPPIRLGAPPEVTVIDLVRVAPSAPDAAGPF
                     "
     gene            3721..5235
                     /locus_tag="PLW16_04360"
     CDS             3721..5235
                     /locus_tag="PLW16_04360"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPB70171.1"
                     /translation="MTKTGFDNERYIREQTAAILGRVERFNNKLYLEFGGKLMYDFHA
                     ARVLPGYDPNAKMRLLQALKDKADLLLCIYAGDIERKKIRADFGITYDSDALKLIDNL
                     RQWGVNVLGVVITRFDRQPAAQLFKNKLERRGIRVFTHPFTRGYPTDVELIVSESGYG
                     ANEYIETGKPLVIVTGPGPGSGKLATCLSQLYHDHQRGLKSGYAKFETFPIWNLPLKH
                     PVNIAYEAATADIRDFNLIDPFHLEAYGETVVNYNRDAEVFPVLRRILEKITGGESFY
                     RSPTDMGVNRAGFAVTDDEVVGEASRQEVIRRYFRYRCEYVMGLTDKETVQRVELFVR
                     NFGLTPEYRRVVVPAREAAREAQEKDKGNEGIYCGAAIELARGQIVTGNNSPLMHAAS
                     SLVLHAVKRLAEIPPKIKLLPATITDSVRNMKTEILNETSVSLDLEETLIALSISATT
                     NPAAQLAVEKLKELRGCEVHMTHIPTPGDEAGLRRLGVNLTTDPNFSTSNLFMD"
     gene            complement(5244..5969)
                     /locus_tag="PLW16_04365"
     CDS             complement(5244..5969)
                     /locus_tag="PLW16_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70172.1"
                     /translation="MPIRQVSYVFEFPTGEIFQYDLRIDATTLSLIPETEEADGTEVT
                     YPAWTRLGHCQCRHCPLSENDQPHCPIAVNVAQLVDAFREVDSALPVKISVHTDERTY
                     LKEGPAQRGMASILGLIMATSGCPHMNFLKPMARFHLPFATSEETIVRSTSMYLLAQY
                     FIARKGEAPDMKLTKLDEAYTRVQKVNFGVSARIRSAAETSRGDASSNAVVILFTVAQ
                     LLSTAIEEKLDTIEDLFKGIVFP"
     gene            6353..7114
                     /gene="hisF"
                     /locus_tag="PLW16_04370"
     CDS             6353..7114
                     /gene="hisF"
                     /locus_tag="PLW16_04370"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPB70173.1"
                     /translation="MKTIKIMPCLDMKNGRVVKGVHFVDIKDAGDPVGNAAFYEREGA
                     DELAMLDIAATLENRPTRLEWVRQVAAVIGIPLTVGGGINSVEDIEMVLAAGAGKVSM
                     NSAAVKNPELVAEAARRFGTDRITIAVDARRNAAMPSGFELVVAGGTTPVGQDAVAWA
                     GRCEDLGAGVILPTSMDGDGTLAGYDLEFTRAIADRVTVPVVASGGAGTLEHFYEGVV
                     TGGAQILLAASVFHFRTFRIREVKDYLRGRGLPVI"
     gene            7150..7917
                     /locus_tag="PLW16_04375"
     CDS             7150..7917
                     /locus_tag="PLW16_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB70174.1"
                     /translation="MEFKSCRAIVTGGASGLGEACVRRLAAAGAKAAIFDVAVERSEK
                     LAAELGPNVIFAQANITSDESAQAAVGKVVDAFGGINVAINCAGVGDPGKVLSKRGPL
                     SLAAFNRVVQINLVGTLNIIRLAVEQMVKNEPNADGEKGVIINTASIAAFDGQIGQAA
                     YAASKAGVVGMTLPIARECAEYGVRVVTIAPGLFDTPMLAGLPDPVRDILASTIPFPH
                     RLGRPEEYAWLAQHIIENTMLNGECIRLDGALRMQAK"
     gene            8187..9851
                     /locus_tag="PLW16_04380"
     CDS             8187..9851
                     /locus_tag="PLW16_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB70175.1"
                     /translation="MILASQEMIKKWTEAGAWGDKTFIDYFKEHVRTQPDKVCLVDPM
                     NKEALVGLKPERLTYKELDRAVDATAEAFVNLGIKKDDIIMVQLPNCWELAMLYLAIT
                     RAGALISPMPMQWRASEHDFIAEMTEAAAVITVEEFGGFKHKEMVEKIQAKRRSIKHI
                     LTLPQIREMTQGEVTGKLDQIHIDPNDVFTLCWSSGTEAQPKGCPLSHNNWLFQTTLC
                     YDSAPILPGDNLITAGPLVNMASIGTVFVEWLRGGGKFVLHHPFDGATFVMQMILEEI
                     NYTLLVPAIVNALLKHPKVDQFDFSKVRSITIGAAPPSLWSVQELKRRWGIEFGNIWG
                     QNEGTGNIAGAKHITDMEMRIDHFPLLGTPGTPWYEGQLAFVTMKLIDPVTKQEVTEE
                     GKVGELWYRGPNVIPGYFKRPDLTEKSFDKDGFFNTGDLFQMKDNNCVGFFERSKDII
                     IRGGFNVSAQEVENLVLGYPKVTDAAAVAMPDEILGERTCIYVVPKPGETITLEEIVA
                     FMKEKGLAVYKIPERLEIIDAIPRNPVGKIMKKVLREDIKQKMKTA"
     gene            9918..10409
                     /locus_tag="PLW16_04385"
     CDS             9918..10409
                     /locus_tag="PLW16_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70176.1"
                     /translation="MRGNIDSVGDMFALTAAKYPDKTALVFQDERFTYGELNARVNRL
                     ARALTAAGVRKGDKVAFLFMNSPEFVVTWLAVAKAGAVGVPLNYGLVPRELKYQIGQA
                     DAVAIVFSPLFMERIAAIRDDLPQVRTYLCNGENGTLDFETLPPATRPMSRRWTSPCM
                     TPT"
     gene            10379..11431
                     /locus_tag="PLW16_04390"
     CDS             10379..11431
                     /locus_tag="PLW16_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70177.1"
                     /translation="MDVSLYDANLILYTAGTTGVPKGAVLTHRNSLFNAFTCCLDYRF
                     TCDDVLQIVPPLFHAAAVNCMLLPGLVLGCTIAIHLRFDPEDMLRTIQQEAVTVTWGP
                     AILWRTLLAYPDFAKYDVRSVRLLGNGGMAMPGEMREKLLAHFPCAAMMDIYGMTELS
                     PAATLLRPEAALAKPLSVGTPMTLTDVRIFDWRGREAAVGEVGEIAVRGNALDGYYKM
                     PEETGQAVKGGFFRTGDLGKRDEDGFITLVDRKRDVITTGGEQVYSREVEEVLQANPR
                     IKESAVIGVADEKWGERVVAIVSLRQETTPDALLAWCRENLAAYKCPRQIHIVNDIPR
                     NAAGKVVKGELRKRFG"
     gene            11470..11763
                     /locus_tag="PLW16_04395"
     CDS             11470..11763
                     /locus_tag="PLW16_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPB70178.1"
                     /translation="MNRPFYQVEASTAEVFSGNPAAVCLLEDWLEDAPLQAVAADNNL
                     SETAFLVPNSDGFDLRWFTPTTERRWRSAAMRPWRARENFGPSGPLSGRADHS"
     gene            11918..13915
                     /locus_tag="PLW16_04400"
     CDS             11918..13915
                     /locus_tag="PLW16_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB70179.1"
                     /translation="MSQIFNLRAEEGIGIVTFDIVGDAMNTWTDAAFEDFDRLMGDVE
                     KEKGLKGLIFISGKPENFLSGANLKTMAQVESADVARTILNTFHGCFTRMGRLGYPVI
                     AAIHGHCLGGGLEFALACTARIAKEGKTTIIGLPECNVGLFPGGGGTQRLPRLIGYPA
                     FELILKGKMLPAVKALELGIIDRIIPADQDLLAAAKAFLTEINAGKAELKRLEQDFSQ
                     VDAIAEMAKQGILKATKGREIPGPMLALDSMHRGVKLSLEEGLELEKENFVKVILSNQ
                     AKGSINTFFIKGMTDKPKSMMTKGFVPKAINKAAVLGFGTMGRGIVIDILRNTKIPVI
                     VKDVPEALEPGKAFLRKILDGMAEKKRLKESVEDLMARLTATSDYIDAFREVDIVIEA
                     VFEEIGVKKQVYEELGKVIREDCIVASNTSSIPITSMAPFVVNPARFGGIHFFSPVWM
                     MQLVEVIRGEQTAQDTIDNLLNFTGLIRKRPIVCRDNPGFVVNALLFPYLKLALEYIE
                     AGNPIEKVDGAVTAFGMPVGPIRLTDEVGIDVPYKAMKGMGFVQPTLKNVVEAGRCGL
                     KKSGKGFFLKDGSVDPEVLPLIAKDAPREVSVEEMQTGFIESMLKVGKDLLDRQIVMS
                     PAMIDVGMIWGTGFPPDKGGPMKWGDLTGLSEKLFGKKFYA"
     gene            complement(13925..14683)
                     /locus_tag="PLW16_04405"
     CDS             complement(13925..14683)
                     /locus_tag="PLW16_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPB70180.1"
                     /translation="MDQMLAPIRAKSLKEVFIERFEALILSGKLEVGQKLPSERELAE
                     ALGVSRPVVHEGLRDLEVRGMVTMKPRVGTVVNDYRLDGSLAILSSLLSHYSGELETK
                     LLDSMFEMRILFETETVRLAALNRTEEQRQVFHRLIGEWDTVDMKDLEATADLYFRFH
                     HHVAMASNNLFYPLLLNSFKNVYLSMARQSFIDAPVVPRVSSCHRRIATAVDAKDPEA
                     AVAAMRDLILFGETHLKGLIEASGRNRGTLPPAP"
     gene            complement(14832..15032)
                     /locus_tag="PLW16_04410"
     CDS             complement(14832..15032)
                     /locus_tag="PLW16_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70181.1"
                     /translation="MAEQILPFLWIGVALMSIVVLVLLSMTLWELRKTVKSVAIISER
                     VAYLTDVKAWWGFLTTLVRRKG"
     gene            complement(15119..15268)
                     /locus_tag="PLW16_04415"
     CDS             complement(15119..15268)
                     /locus_tag="PLW16_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70182.1"
                     /translation="MTKKKKFFLSVIVVAILGIVFYRFLPPHRKQQLRFLLKQAPSLP
                     GRYMV"
     gene            complement(15292..16833)
                     /locus_tag="PLW16_04420"
     CDS             complement(15292..16833)
                     /locus_tag="PLW16_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70183.1"
                     /translation="MQDRIGKESRLMADYDVIVIGAGLGGLSSGALLAHRGLKVLVLE
                     QSGRIGGCCSTFERDGYRHDVGASIVEITQPIELVFKALGTSFGEEVDLIPCDPIFSV
                     MYRNGERITVEKSIEATGKLIASLDPADGRRWFDFCDYCSDMMDVLLDSILVEPADTL
                     GDMARVVKKNPALLRYLPNFMSTYQDLIQKYFKSDQVQRTMAYQSLYMGMPPALCPGP
                     FAMIPYSEHRGIWYPRGGMIRIPEALVRCGQKDGMELRLNTPVTSVIVRNGRAKGVVL
                     EDGREISSRMVVSNVNPRMLYLDLIGEQHLPWLTRTGVKSLKYGKSLCMVYVGVDCKP
                     PLDAHHSYIAVSMQEVNDFWAKHVETGVMLPANANYGLVCWPSHSDDSLAPEGHHVLN
                     VIPESFYHLKGTNWDAFKDRFIDEYIDYLSEKLVPDLKGHITHAIGSSPVDFERHLRL
                     PEGSIYCFHQDASHQAVFRPSSKSKCIDGLYLTGSGTHPGGGVPTTMSSGYIAYSQIL
                     KHERC"
     gene            complement(16930..17070)
                     /locus_tag="PLW16_04425"
     CDS             complement(16930..17070)
                     /locus_tag="PLW16_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70184.1"
                     /translation="MNIKKILLLAGAAVAVYVYVKQPERKKRFLREILRQVPYLIPRY
                     FA"
     gene            complement(17081..18583)
                     /locus_tag="PLW16_04430"
     CDS             complement(17081..18583)
                     /locus_tag="PLW16_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70185.1"
                     /translation="MTDYDVIVIGAGCGGLSVGALLAKQGRKVLVLEQSDRIGGCCST
                     FQKEGYTFDLGASLIEDAEVIDWCFQRLGTTLDREVDLVSCDPVYDVIMKDGTRLKYP
                     LSAEASARNIGALAPEDVKGWHAYADYMQGFLDAALKGFFLAPANTQGDLVNLFAKTP
                     KLLKYGPMFASSYQGVMEKYFQDTRIRESIAFQSFYGGLPPDSCPGYIAMIPWSEHAG
                     IYYSKGGMIGIPRALERLGQNFGLTVRLKTRVSRVIVRNRRALGVALADGTEITSDLV
                     VSDINAKLLYLKMIGEEHLPWLARVGIKSYEYSMATPMLYLGVDYTPPLEGHHTLISR
                     PMDELNDYWWNVYKKDRYAEEHFGIVSWTSHSDPGLAPQEGHHVLVLTLEPGPYRLHG
                     TTWDDFKAALTEQILDYMSKRYIPGLAEHVRVAELSTPVDFERRLLSPEGAIYALRQD
                     LTSGVTFRPAAKSKSIAGLYLVGASTHPGGGVPTTIASGMIAADLIQRYE"
     gene            19059..20873
                     /gene="typA"
                     /locus_tag="PLW16_04435"
     CDS             19059..20873
                     /gene="typA"
                     /locus_tag="PLW16_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPB70186.1"
                     /translation="MSSPRKKQIRNIAIISHVDHGKTTLVDAMLRQTGTFRIHQVVEE
                     RVMDSLDLEKERGITITAKNTAIRYGEVKINIVDTPGHADFGGEVERSLNMVDGAILL
                     VDASEGPLPQTRFVVKKALAAKLPIILVINKIDRSDARIEAVLNEVYDLFIDLDADEA
                     QIEFAVLYTNAKAGVAHEVQGDGSADFKPLFETILKRIPGPEGDDGAAPQFLVTNLDY
                     DPYVGQLAIGRLMNGTLEINQGYTLCGEHEDTPGVRFSALYTFHGLQRKAVERVEAGD
                     IAAFAGVEGVNIGDTVAALVTPRPLPRIRVDEPTVSMIFYVNNGPFAGKEGKYLTSRH
                     LRDRLERETLKNVALRFQPLERKDAFEICGRGELQMAVLIETMRREGYECMVSRPRVI
                     TREENGKTLEPVERLFIDVPEVYIGILTEKLAARKGRMTLLVNRGSGRVNLEFIIPSR
                     GLIGFRSHFMTDTKGSGVMNTLFEGYEPWAGPIPQRVSGALVADRVGRVNTYASLAMA
                     DRGEMFVPVGTEVYGGMIVGERNRTGDLNVNIVKEKKLTNMRSSTSEATVTLRPPRLL
                     SLDQSIEFIAEDELVEVTPHSIRLRKLDLVAYTRTVRS"
     gene            21003..23645
                     /locus_tag="PLW16_04440"
     CDS             21003..23645
                     /locus_tag="PLW16_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB70187.1"
                     /translation="MTYVMGLDAGGSGGRCLLWEVGSGRVFSASAGWSHPVAPGTAGL
                     GFNLDLDDVWAKLGRAVREVLSRSGATPEDVLAISATSMRHTTVVLDAAGDVLLAAPN
                     HDARALGEGLILGAEHGGRVHGIGGHWPSPIFTGARLLWMKAAAPEAFGRAARVLTLS
                     DWLAYRLSGVVAAERSQAGETLLFDQVRREWAEDLIHDLGLPAEVFPALVDAGTRLGG
                     LTAAAALHLGLAEGTPVVAGGADTQCGLLGAGAVRAGDLGVIAGTSTPLQIVTARPVF
                     DPEGRLWNGQHVIPGLYVTESNGMATGEVLAWVAAMIYPDCARPMEALLADAARSTPG
                     AEGVSSTLGAHIFDGRTIGIPVGNLTLSHMASPGSSRRELGRAVLEGLAFSVAANLKQ
                     IEAVTGQAVPTLKITGGLSRSPLWTEIVSDVTGRAVQVPATHESSSLGAAVCAAVGVG
                     AFASFGAAAARMVSIAREQAPGGDAESYRKLYAGWKEAHGLRAAADSHMANLLTTAMM
                     ERPVSRAKPAPGFRPRIKVTASLDDMVLDRLRQYGEVDYANWRETAKVYSGEDVIGLL
                     KGFHAFITEMDVVALDAVRELPDLRFIATCRGNPVNVDVEACRAFGIPVVYTPGRNAD
                     AVADLVIAFMLMMARRLPEASAFLKLPGGKAGDLNRMAEAYLNYQGRELWGKTVGLVG
                     LGQVGAGVAARLRPFGARVLYYDPFVADGALFSAVRTGLEELLAESDFVSLHVPLSDA
                     TRGLLDAAALERMKPGACLVNTARAGLVDEAALSRVLESGRLAGAALDVFSVEPPAAD
                     NPLVASPRVIVTPHLGGNTVEVGTHQGIIAAEQLEQLLRGETPEFILNREVMTAFSWT
                     GPRPEPGVEELARVAQGPGPTMTS"
     gene            complement(23780..25372)
                     /locus_tag="PLW16_04445"
     CDS             complement(23780..25372)
                     /locus_tag="PLW16_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB70188.1"
                     /translation="MGWQDLNLTWHYVEKWAQEKPGAEALVFGDERLSWKDFKDRMDR
                     IARAYLAIGVEKGDRIALLSAARNEFLMTYMAAGKVGGMWLGLNPKFTLDELRYQIGD
                     SKPVVLIAVRTFLGNDLAATIAALKEEFPFIKRVLVIGDPVEGTENFQDFIDRPRPEL
                     DAAMEKRAADIRPGDDALLMYTSGSTGKPKGVVHTHGSIVENIKVEVVKFYVDEETRC
                     LLHFPINHVAADVEIGFAAVMAGGCLVFMEQFDPVGALKTIEKETITMFGQVPVMFLL
                     QMKQPEFFQTDFSHVRLMAWAGAAAPKIMIDVLSGICAKTGGMMITGYGSTEVCGFVT
                     YTEKGDDPNTLLKTAGKIAPPFELKIVDDDRRELPDGQIGEIAVRGPFMFKGYLNNSE
                     ATAEVLDRNGWYYTSDLAFKDARGYITITGRKSEMFKTGGENVYPREIEEIIETHPSV
                     LFAAVMGVPDEIYQEVGWAFVMPSPGKEVTEEELRELCRSKLANFKVPKKFFVRPLLP
                     LLASGKVSKLALKEEIKGLLKS"
     gene            complement(25467..26345)
                     /locus_tag="PLW16_04450"
     CDS             complement(25467..26345)
                     /locus_tag="PLW16_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB70189.1"
                     /translation="MEISSRFVGSPLKPLDARITWRQTTNYAAALDDANPRYLDDSDP
                     GGLVAPPMFAVAVTWPISERIPDFIETSDFPFEILATQVHYTEHLEFFRLIRPDDRLT
                     VQGRVAAILPHKAGTLMVLRYDARDEAGAPVFTEHIGGMMRGVRCTDDGAGAETLPET
                     LPQPEPGDPLWETRIRIERTAPFVYDGCTNIHFPIHTSVKFAREVGLPDIIYQGTATL
                     ALGVRDVIKREAGGDPERVRSIACRFTGMVIPGSEIRVRLLESRSDGDRTHLFFSILN
                     TEGKRAVSDGVVTLLR"
     gene            complement(26498..27985)
                     /locus_tag="PLW16_04455"
     CDS             complement(26498..27985)
                     /locus_tag="PLW16_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB70190.1"
                     /translation="MTQTLEDSPLYPIVNCRSMAYFGASNNLMAMGSLILSAQLATGF
                     NGPIYPVHPTEDTVQGLKAYRSVADLPEVPDMALFVLPPRLVAETLAACGEKGIRRAV
                     IVSGGFQEVGPEGAERQKEVVAVAARYGIRFIGPNCIGVVNTHHRLNTTLFLYDEKPG
                     FIGLASQSGSFVAQMFNHLTRHAINFSTAFSVGNEANLDIVDCMEYLAADPRTKVIGL
                     YIEGIRRGEAFVKAARAIAPRKPIVAYYVGGSETGSRAGFSHTGAMAGPDAVYDGIFR
                     QCDILRARSVTELFDFCWVLGAMPLPAGRRLVIQTHSGGPGAAAADHCGREGLDLPLL
                     SEKTVERLKPFLPHTASFGNPVDMTFARNQADYFTAIPDVLLREPGADMLLLYILVPS
                     LMIQRSLEMMGLPPEDIAAKTDEIIEAQTGALAALVGTHGRPVIGYTYRGIEETFCRQ
                     LMEKGVPVFAGPERAARALTALVQYRKIRNRQDGSAMDKVGTVTS"
     gene            28352..30283
                     /locus_tag="PLW16_04460"
     CDS             28352..30283
                     /locus_tag="PLW16_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70191.1"
                     /translation="MLEHLFSPVTINGMTLKNRAVMPPMGTGYGNADGTASERLMHYL
                     AARARGGAGLIITEICAPDPRGKGFPNELGAWSDDLIPSLGKISAAVHREGAKVALQL
                     HHAGRETFAAAAGGLPEAPSAIPSAILGQPCEAMSLARIAELVAAFARAAARAKEAGF
                     DTVELHGAHGYLLNQFLSPFSNQREDAYGGSEENRSRFVLETLQAVRAAVGPAFPVLI
                     RISGDELIRGGYDLDFTRRLAPQLVAAGADAIHVSVGVYSTPGNLSIASMDTATGFNL
                     FRARAIREAAGVPVIGVGRIQRPADADRAIAAGDADLISFGRQHLADPDFISKTLAGR
                     EEDIRWCVACNQGCIERLSFEMRSTTCTFNPECGQEYRGAAAAVTGAPRLWVIGAGPA
                     GLAAALASRERGFAVEIFEKATVPGGQLQPASRPPHKQPFMDWVHWAVRQLEKRGVRL
                     HYNEEMRAEILRRDRPDAVILASGASPVVAKIPGIDGPKVCDARDVLMGKIAPAAPAV
                     VLGAGYVGMETADYLVARGIDVVLLEMQPAHPVGKLTAHAYWLHRRIREGRGRIVLGA
                     TVTRVEKDAVFYRQGDEERRVPAALVVTAMGARPDNALEGPLVQLGISYRVVGDAVTP
                     RRLLEAIHEGHQAGRDIQG"
     gene            30280..31050
                     /locus_tag="PLW16_04465"
     CDS             30280..31050
                     /locus_tag="PLW16_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB70192.1"
                     /translation="MITAGIDIGSMTTKGAIVADGRILMTCVRFTGYNAEAAGEKVFR
                     ELLDALDLEASRVDRVVATGYGRKAAAFAQATVTEITCHAAGAHHLDPRVRSVIDIGG
                     QDSKAIALNPQGKVRDFAMNDKCAAGTGRFLEVMARAMEVDLDAFGVLSLLSRKPAQI
                     SSTCTVFAESEVISLIAGGESRENIVAGIHEAVASRVVAMARRLGLVAPIMMTGGVAK
                     NAGVVKALENKLGHVIEVSPYAQENGAIGAALLAALRI"
     gene            complement(30977..31969)
                     /locus_tag="PLW16_04470"
     CDS             complement(30977..31969)
                     /locus_tag="PLW16_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB70193.1"
                     /translation="MSRNRSCKRTAPSEAPVFGLGQCALDYIGIIPAYPPADVKCEFT
                     NLEIQGGGPVATALAALRRWGLPAYFAGVAGNDAFGAAIAASMRDAGIDTGGLRVREG
                     HLSQFAFVAAEPGTARRTIFWQRPTGPPLSPEELDLERLRTSRALHTDGFFPEASLFA
                     CTQAREAGIPVVCDAGSLRDGMLEIAGLSGCFIASEAFSQALTGNPMETCRRLAGLGC
                     PFVGVTLGAKGYVALVGGRWIRTPAYPVRTVDTTGCGDVFHAAVTYGIVHGLAPETCL
                     DLGAWAAARVSTRLGGREGIPHEQELERHLAKSATPPEGPRRSRRSPGRRGTPR"
     gene            32184..32990
                     /locus_tag="PLW16_04475"
     CDS             32184..32990
                     /locus_tag="PLW16_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB70194.1"
                     /translation="MKRILFHGSPGLILAVVILLGSCEVQNRFLYFPSADRPPARSLA
                     AENMRLWQETSGDYRGLTADCEADAPHGTIVLFHGNGGTALDRGFYLPPLMRRGFRVI
                     LAEYPRYGGRPGRLGEEALVADGRETVGWAYAQYGRPVYLLGESLGAGVAAAVAGKSP
                     VPIAGVILMTPWDTLADVARTQVPFLPAGLFLTDTYDSIGNLRLFKGKIAVIGAERDG
                     IIPVGHARRLYASLPEVRKRLWVIRGAGHNDWPLYAGASLWEEVTNFVKE"
     gene            33240..34556
                     /locus_tag="PLW16_04480"
     CDS             33240..34556
                     /locus_tag="PLW16_04480"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB70195.1"
                     /translation="MEENHQRTSTAGGRGGKKLRRVMKKSSAVSSTIVFLWLLIGCGG
                     VIIHQTSQPDLQPIPYNDVKMRAQKVFFEGHNPSVRFRGTGKAIKFTEDGFRIGTDPQ
                     VFCEYEVTGNPYVKDWGPAALDSQFGVHLDGCDVQTWFKQKESAILFAQLLYTLKQQA
                     VENKVARNLPAGTASPIETKTIKTDIDELPAIHSEPQQNAYAVVIGIEHYRSIPKSDY
                     SRNDAHLVKGYLKTLGFQERNIDLITDEKATKTDIEKSLEAWLPNRVRKDSRVFVYFS
                     GHGAPEPATGEAYLVPYDGDPNYLPVTGYRLKRLYDSLGKLEAKEIIVVLDSCFSGGG
                     GRSVLAKGARPLVMMAPTGALPSNMAVLSATQGSQISTSSPEKGHGVLTYYFLKAIKD
                     GKKDLAEIYEHILPQVEDDAKQLNVQQSPSIRPDTEKIKGRFYLVK"
     gene            34562..35377
                     /locus_tag="PLW16_04485"
     CDS             34562..35377
                     /locus_tag="PLW16_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB70196.1"
                     /translation="MRRCLVFLFLLVFPCVGLAADNPVWVEAEGEARFGDFDTLKEVK
                     DRARRDAEAKAIEQAVGVFIRSHTLISNSQVAEDLIYAAVRGKITKEIVISSDWDHQD
                     RHLYRTRINARVEPVYPGKGDGLSVNLSLSKTDLKEHEEVRIFYRANKDAYIYIFSIA
                     ADGSVTLLLPNSQMPDHFFQGGKAYEFPPADSRIRLKALFLPGFQTDSAEERIKIIAT
                     RNNEELLPLGFREGIFHAYDARSTGMINDLVRRLNQFEPTDWAEATAIYTLGR"
     gene            35486..36952
                     /locus_tag="PLW16_04490"
     CDS             35486..36952
                     /locus_tag="PLW16_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB70197.1"
                     /translation="MAPPRPLLFPIVLMVFLAGCVAKSPLTVAIEQGNVAVVKTLVDQ
                     GANLRELADCGSGMSTIRTTPLECAVWFDRTEIIHLLIDKGADPSEQDYYGGSTPLST
                     AAAYGINRSAKVLLERGANPNAGKPLSNAAYHGNIELMKLLVAKGADIDTAIEELKTR
                     YDSWLPERNKKIDSAIRLLDKLKPKPVVAIQAPAPSAASPQNITKEDIATIVQEAVEK
                     AGRKEARPGVAVSSEIDRPQWTPAERIMGDKDLAIVIGIEGYQSLPKSDYSYDDAMLV
                     KDYLKALGFKERNTEFLTDERATKSSIEKTVEVWLRNKTQRDSRIVIYYSGHGSPDPV
                     TGEAYIVPYDGDPNYLSVTGYPLKRLYEKVGALPVKETVIVLDACFSGAGGRSVLAKG
                     TRPLVISTMEAPRYGNMVVLTATQGAQISTSSPEKGHGIFTYYFLKALQEGKKSIADI
                     YEYMKPRVEDDAKTLNVQQSPSVSPDVKTLRGRFILRN"
     gene            complement(37383..37577)
                     /locus_tag="PLW16_04495"
     CDS             complement(37383..37577)
                     /locus_tag="PLW16_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70198.1"
                     /translation="MNSRIIGLRMASILFGLMTVGQLTRLFVRPEIIVEGYVFPLWPS
                     AVAVVVLGGLCLWLWHLSRG"
     gene            complement(37718..38245)
                     /locus_tag="PLW16_04500"
     CDS             complement(37718..38245)
                     /locus_tag="PLW16_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPB70199.1"
                     /translation="MKKIAIVLLLLLTGCVGIPEQVKPVDHFKLEKYLGRWYEIARLD
                     HSFERGLTRTTADYSLRDDGGVRVLNRGYAPKEKAWKEAEGKAYFVKGPDQGYLKVSF
                     FGPFYGSYIIFELDRENYQYALVCGPDKSYLWILARAPEMKQDVKDALVAKAAGLGFD
                     TGNLIFVDHREWPGG"
     gene            38673..39158
                     /locus_tag="PLW16_04505"
     CDS             38673..39158
                     /locus_tag="PLW16_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70200.1"
                     /translation="MLSSADQDKLIVIMKAMEEHELAVAELYQTAAVASPSDQAFWNG
                     LAAMEKTHADNLERMIAQVQAKASRFEMGRPFNMAALKTAQEGIRQNISKIARGALSR
                     LQMLVVARDIEQSLIEAQYGSVVKTNDVEYQTLLKEIVDQTYGHRKAIQEQIDKAQKG
                     A"
     gene            complement(39155..39988)
                     /locus_tag="PLW16_04510"
     CDS             complement(39155..39988)
                     /locus_tag="PLW16_04510"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70201.1"
                     /translation="MKTQRTRYDRPVEVAPGIHWVGFHDGYSNFHCNPYLIIEGDKAV
                     LIDAGSRADFPVVMRKILQTGIAPEQIVALIYQHPDPDLCGSMANMVDLCANEALTIL
                     SDRANNVFLSFYLERERRGLLRSMDDRGMVFSFNGRELRFFKTPFAHTAGSLVTYDPR
                     TRTLFSSDLFGSYSQRWDLFLRLDDQCRSCDAYDTDGPCAYGKPYCPLTDLLAFHRQV
                     MPSDKALHRAMKVIGGVGAERIAPQHGSIIDQQEDIAFLTGCLAKLTGVGIDGLSGNG
                     A"
     gene            complement(39963..40706)
                     /locus_tag="PLW16_04515"
     CDS             complement(39963..40706)
                     /locus_tag="PLW16_04515"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB70202.1"
                     /translation="MVDYLQSVATIQDGGIQQRLLKELIRQYVALEKRVDELLKNTLP
                     AEVAEEIKESGSFAPRTCNCTILFADICGFTQMASRLSGEKLVRMLDLIVRGADDLMR
                     RYRGTKVKTIGDAYMAVFGAPRPDPDHPSLAIQAGLAMHEQLKAFRAGGDLKIETRIG
                     IHTGTVIAAVVGEERMQFDIFGDNVNIASRFETGGTPGRVNCSHETYLLTRNRFFFEE
                     RGEIPLKHRGRMKAYFATGERHENTANAL"
     gene            41065..41574
                     /locus_tag="PLW16_04520"
     CDS             41065..41574
                     /locus_tag="PLW16_04520"
                     /inference="COORDINATES: protein motif:HMM:NF017764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HPB70203.1"
                     /translation="MKKIAILTGGAILLLILLLAVWLIFRPDRGTTGAVNTQFRWIGP
                     NDKIVVDGFDDPKVRGVACHIARAQTGGVKGALSVAEDASDASIACRQTGPIKFLDEF
                     KDGERVFDEQRSLLFKSLQVVRFYDRKRNVLVYLSYSDRVLTGSPKNSISTVPIMPWP
                     AAGDSAMMR"
     gene            41832..43205
                     /locus_tag="PLW16_04525"
     CDS             41832..43205
                     /locus_tag="PLW16_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB70204.1"
                     /translation="MDVYGSLVEIVGEEFVSDQEEELYIYSRDSGAQEPRKADYVVAP
                     KKVEEVQKIVLLANREKIPITPMGGGLTLSGLAVPIKGGIVLDMKRMDRIIEINEKSR
                     YAVLEAGVTQGALHSHLNKHYPHLEHSMPDAPPMATIVGNVVIQGHGHLSVSRGINSQ
                     MVNGMEVVLPTGEVCKLGSCSVSPHWFTRDPLPDLMGLFIGWLGTTGIITKISLQLLP
                     KPKFRDLLTFTSNELDLLPDFIFDITQLNLLDNLFIISQEWPPWAQGILYVPIVSAQD
                     EEEFALKIKLHKELLRKYEGKITLVEGKHAERMREARLPVPPVTLGVMAADTKQGGGF
                     EYTGGIVPIEKVPEASSRSVEIAHKYGQPYLLGYQLLTGGHSMMCGLAYCLNRADDED
                     VEKVRKAIDETNRMILDVGGVLWKAEAPAQRLMMQRMDPNTIELMKRVLRALDPNAIM
                     NPGNWSE"
     gene            43206..44387
                     /locus_tag="PLW16_04530"
     CDS             43206..44387
                     /locus_tag="PLW16_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB70205.1"
                     /translation="MALADYEGMIHRCFRCGYCKFTSDYSYVGFNCPMYNKVRLESYS
                     PGGLMWLIHAALVKEKLKPSKHLSEILYSCTLCRNCSEECRFQFHEDIVHVLKAAREA
                     MVEKQLVPPMVSQFLKNVYDYGNPYRELKENRGHWAENTGIRQYEKGDEFLYYVGCVG
                     SYDTVSQKAARALGRVLLKSGLSFGILGSREICDGNEVDMLGEKGLFEFLKEENTRTF
                     KELGVKKIVTLSPHSYHALKNSYSDEFEVFHYTQLFRDLIKSGALDVSGGFRTKVTYH
                     DPCFLGRYNHEYDAPREILTAIPGIELIEMERAKENAFCCGGGGGNFYTDILGGGEST
                     PSRIRVMEARETGAKILAVSCPVCMMMLEDALKAEGLENELAIKDIAEIMDAVCGDSG
                     A"
     gene            complement(44403..>45558)
                     /locus_tag="PLW16_04535"
     CDS             complement(44403..>45558)
                     /locus_tag="PLW16_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB70206.1"
                     /translation="ENYRRLENLHAENAFDIKEFSERKTGGELGCQAYEFVEGSELHR
                     VRLRYYPIPQGIKPNGKIIYMVTPLINRPELFDLACDKSVVEALLKKGYMIYLVDHGN
                     PGWEETELGLDFYGKTIPDLYIDLIKKRHPQNEIYIMGYCMGGTLILPYLARRAEERM
                     AAGLPMDIRKIALMASPVKFDDGDSGHGPIRTLIRRDYDPYLMKELFGAVNIPPQIIE
                     AGMNEIQPGVQYTVVLGFYGRAVSREAILDAAPFLFWLTRGTKFPIHCHIEWIQKIFL
                     GNEIFEGKYALPSANPKFDGKPVNMKILKKAGVAIFDYRGSRDPIAPAGSCVASETWG
                     QVNHGKIRLPKGPVNRTIEKNIGHIFVVSRKHLADYLKDVERFYRDEKPS"
BASE COUNT         8711 a        13720 c        14690 g         8437 t
ORIGIN      
        1 ccttttacga cacggacgat ctggtccggg agcggacggg gatgtcggtc cggcggatcg
       61 ttgcggaaca gggatgggcg tccttccgtt cggctgaaag ggcggtgatc gcggacctcg
      121 cgttcttgaa gggcgcggtt gtcgcgctgg ggggcggcgt cgtgctggac cccgagaacg
      181 tggccgccgt gaaggcgaac ggccgggtga tctggctcgt cgccgatgcg cggacgatcg
      241 tcgcccggat gggggaggac cctgtcagcg cgactcagcg cccgtccctc cggggggtgg
      301 accggctgaa ggaaacggtg cagatactcg ccgagcggac gccggtctat cgggcggccg
      361 ccgattttac ggttgacacg acggggaaaa cggtggatga aatcgcagcg gagatcggcg
      421 gatttctggc gcgcagccct gacgcaactc cacccgacgg aacggatcca tgcggatcgt
      481 gatcccgccg cacgcggggc atattccggc cctgtgcgct tctcgcgcgg tggggcaggg
      541 gcctccggaa aatctccccg gcgcgtttcc gggatgcgct gcgccggggt ccgatgaaga
      601 ggagttatcc tgatgtccgg caatacgaca ggcattgttt ttcgggtgac cacctgggga
      661 gagtcccacg gggaggccgt gggggcggtg gtggacggct gccctcccgg catcgcgctc
      721 gcggccgaag caatccagcg ggaactcgac cgccgcaggc cggggagcga cgccctggcc
      781 tccccccgcc gggaggaaga ctgggtggag atcctctccg gcgtgttcga ggggagaaca
      841 accggcacgc ccatcgcgct gaccatccgg aacagcgacg ccgcgagcgc cgcgtatgac
      901 gacctgaggg acgtcttccg gccgggtcat ggggatttca cctactggaa gaaatacggc
      961 ctccgggacc accggggcgg gggacgcgct tcgggacgag agacggcggc ccgcgtggcg
     1021 gccggggccg tcgcccgtcg ggtcatcgca ccggccggca ttaccgtcgc ggcgttcacg
     1081 cgggccctgg gcggcatcgt cgcgaagagc gacccgcccg gcgacgcgga ccggaaccgg
     1141 ctccgctgcc ccgacgccgc ggcggcggag cggatggagc ggcggctggc cggcgcgaag
     1201 cggcaggggg acaccctggg cggtgtcgcg gaaatttgca ttcacggatg tccgccgggt
     1261 ctgggcgagc cggtcttcga caagctcgac gccgatctgg cccgcgccct tatggggatc
     1321 gggacggtca agggcgtgga gataggcgcc ggcttcgcgg ccgccgcgat gaagggctcc
     1381 cgctgcaacg atcccatcgg gccggcgggg tttgcctcca accacgccgg gggcgtcctg
     1441 gccggcattt ccaacggaga ggccatcctt ctccgcgcgg cgtgcaaacc gatcccctcc
     1501 atcgggcggg cgcaggagac ggtcgacgtc cgggggaacc cggcgacact cgccgtccgg
     1561 ggacgccacg atgtgtgtgt catcccgagg atccttccgg tctgcgaggc gatggtctgc
     1621 ctcgtcctgg cggatcatct cctgcggcag aaagcgatcc gtccatgaac cctttcgaaa
     1681 tcggcatcgt cggggggacg ggcgggatcg gccgctggtt tgcggcgttc tttgagtcgg
     1741 agggctatcc cgttcacgtg gtcggacgta ggacggactt gaccttccgg gatctggcgg
     1801 agcggtgtcg ggtcgtcatc gtggcggtgc ccatcggcgt gacgcaagag gtcatccacc
     1861 gggtgggacc gctcctcgat ccgggcgcgc tcctgatgga cctgacctcc ttcaaggccg
     1921 caccggtccg cgcgatgctc gacgcgtcgg ccgcggaggt gatcgggtgc catcccctct
     1981 tcgggccgga cgtcccctcg ctcgagggac agaacgtcgc gatctgtccc gcgcggggag
     2041 agcgctggct cccctggctg cgcgaccgtc tcgggaagaa cggcgcccgg atcgtcgaga
     2101 cgacgccgga gcgccatgac cgcatgatgg ccctggttca ggccctgaat cacctggata
     2161 cgattgccct cgggttgacc ctgagggagg cgggcgtttc cccggcggac ctggaaccct
     2221 ttaccacccc cattttccgg atgaaggcgg cgatcgtcga aaaggtcttc ggcgccagcc
     2281 cccgcctcta cgcggaactg atcgccggca atccacacac ggacgagctt ctggcaatct
     2341 atgagacgca actcgcgcgc ctcaaggacc tgctggcgcg aaaggacatc gacggtttcg
     2401 cggccgccct gaatccgggg aaggggtcgc catgaaggga ctggccctgt ttctcctcgc
     2461 cttcctgatc ctctacggcg gtctccacgc ttacgccttc ctcaaggtcc gggcggcctt
     2521 tccgatcccg ccgccgctcc atgtcggcct gatccttctc ctggggctgc tgtttctggc
     2581 gccgctgctg atccgctatt ccgaaggggc cgggttcgaa acgctggccc ggggactgtc
     2641 ccacgtgggc tatggctgga tggggttcct gttcctgttc tgcgccgccg ccctctgcgt
     2701 cgatctcttc cgggtcctga tctccgcggg gggagtggcg ttcaaacaga cgttggcccc
     2761 gctgctgcct tcgccccggc tggtctttct cattcccctg atcgtggccg ctgtcgcgat
     2821 ggcgtacggg tacggggagg ccctgagcat ccgcacggag cggctcgtga tccggaccgc
     2881 caaactcccc gccggggtgg atcgcctgcg gatcgtccag atctccgaca tccatatcgg
     2941 cctgatcgtc cgggaggacc ggctcgataa gatcgtggcg gcggtgcagg cggccgggcc
     3001 ggacatcgtg gtgtccacgg gagacctggt ggacagtcag ctcgaccata tccgcaccgc
     3061 catggagcgc ttgagggcgc tcccggcgcg gtacggaaaa tacgccatca cgggcaatca
     3121 cgagttttac gccggtctcg acgatgccct cgcctttacg gagggggcgg gattcacgat
     3181 gctccgggac gagggcgtcc tggcggccgg agcggttcag atcgtcggca tggacgacgt
     3241 cccggggcac gggttggcat ccccgaccgt gagtcccgag cagacgctgc tccgggggct
     3301 tgcgcccgac cgtttcaccc ttctactcaa gcacaagccc gttgtcgaaa aggagagctt
     3361 gggccggttc gacctgcagt tgtcggggca cacgcaccgg ggccagctct ttccgttcag
     3421 cctgatcacc cgcctgtttt tccccttcca cgcgggcgcc tacgacctgc ccggtggggg
     3481 aatcctctac gtcagccgtg ggacggggac ctgggggcct cccatccgcc tgggcgcgcc
     3541 gccggaggtg accgtcatcg atctcgtccg cgtcgcgccc tccgctcccg atgccgccgg
     3601 ccctttctga cgaccccgca caaaccggaa tcgggccggg gagatcgtca tctttgcccg
     3661 ctgacaaatg ggggcggatg tggtagggaa gaacaacttt caagcgaggg cggggtgacg
     3721 atgaccaaga cggggttcga caacgagcgg tatatccggg agcagacggc ggcgatcctg
     3781 gggcgggtgg agcggttcaa caacaagctc tacctggaat tcggcggcaa gctgatgtac
     3841 gatttccatg cggcgcgcgt cctgcccggc tacgacccca acgccaagat gcggctcctg
     3901 caggccctca aggacaaagc ggacctgctg ctctgcatct acgccggcga catcgaacgc
     3961 aagaagatcc gggccgattt cggcatcacc tacgattccg atgctctcaa gctgatcgac
     4021 aacctccggc agtggggggt caacgtcctc ggcgtcgtga tcacccgctt cgaccgccag
     4081 ccagcggccc aactcttcaa gaacaagctc gagcgccgcg ggatacgggt cttcacccac
     4141 ccgttcacgc gcggctatcc cacggacgtg gagctgatcg tcagcgagtc cggctacggc
     4201 gccaacgagt acatcgaaac gggaaaaccc ctggtcatcg tcacgggacc gggacccggg
     4261 agcgggaagc tcgccacctg cctgtcccag ctctaccacg accaccagcg gggtttgaaa
     4321 tccggctacg ccaagttcga gaccttcccc atctggaacc ttcccctgaa gcacccggtg
     4381 aacatcgcct acgaggcggc cacggcggac atccgggact tcaacctgat cgaccccttc
     4441 catctggagg cgtacggcga aacggtggtc aattacaacc gggacgctga ggtctttccg
     4501 gtcttgcgcc ggattctgga aaaaatcacc ggcggggagt ctttctaccg gtcgccgacg
     4561 gacatgggcg tcaaccgggc ggggttcgcc gtcacggacg acgaagtcgt cggtgaggcg
     4621 tcacggcagg aggtcatccg ccggtatttc cgttaccgtt gcgaatacgt gatggggctg
     4681 acggacaagg agacggtcca gcgggtggag cttttcgtcc ggaatttcgg gctgacgccg
     4741 gagtatcggc gagtggtcgt gccggcccgc gaggcggccc gggaggccca ggagaaggac
     4801 aagggcaacg agggcattta ctgcggggcg gcgatcgaac tcgcccgggg acagatcgtc
     4861 acgggaaaca attcaccgct catgcacgcc gcctccagcc ttgtcctcca cgcggtcaag
     4921 cggctggccg agattccgcc gaagatcaaa ttgctccccg ccacgatcac cgactccgtc
     4981 cggaacatga agacggagat cctgaacgag acctcggtca gcctcgacct cgaagagacc
     5041 ctcatcgcgc tcagcatcag cgccacgacc aaccccgccg cgcagctggc cgtggaaaag
     5101 ctgaaggaac tgagggggtg cgaggtgcat atgacccaca ttcccacgcc gggggacgag
     5161 gccgggctcc ggcggctcgg cgtcaacctc accaccgacc ccaacttttc caccagcaac
     5221 cttttcatgg attgagcggg gcgtcagggg aaaacgatgc ctttgaacag gtcttcgatg
     5281 gtgtcgagtt tctcctcgat ggccgtgctc agcagttgag cgaccgtgaa caggatcacc
     5341 acggcattgc tggaggcgtc tccacggctg gtttcggccg cggagcggat ccgcgcgctg
     5401 accccgaagt tcactttttg cacccgtgta taggcctcat ccagcttggt cagcttcata
     5461 tccggcgcct cgcccttgcg ggcgatgaag tactgggcca gcaggtacat ggaggtggag
     5521 cggacgatcg tctcctctga ggtggcaaag gggaggtgga accgggccat cggtttgaga
     5581 aaattcatgt gggggcagcc gctggtggcc atgatgaggc cgaggatgga agccatgccg
     5641 cgctgggccg gcccttcctt gaggtacgtc cgctcgtcgg tatggacgga gatcttgacg
     5701 ggcagggcgg aatcgacctc ccggaaggca tccacaagct gtgcgacgtt aacggcaatg
     5761 ggacagtggg gctggtcgtt ttcggaaagg gggcagtgcc ggcactgaca gtggcccagg
     5821 cgggtccacg ccggatacgt cacctccgtc ccgtccgcct cctccgtttc cggaatgaga
     5881 gagagggtcg tggcatcgat acgcagatcg tactggaaga tttctccggt cggaaactcg
     5941 aagacgtagg aaacctgacg aatcggcata cgtgctcctt tccatgaatg aaggtgatgt
     6001 ttatcataag caaatcacct acaaagtcaa cgagaaatcc cgaagtcccg tcgccacgaa
     6061 ccccgtcccg gtcgctcgat ctttgccttg acttttgaat cgggatggat aaaagtaacg
     6121 cgccaaccgg gaggcatcac cggtccggcc aggcgggggc ggtctttgcc gcgcttcccg
     6181 tcggtgtcgt ccccggaccg ggacttggag ccccacgggg tggcggcaaa tccatttccc
     6241 cctatcgatg cgcgtcgccg gagaaaccct tgaagccgtt gatgttcttg cattcacttt
     6301 ttcggccggt tccggtcgtg tgcgtcaaat tccagaggca aggggagcag ccatgaaaac
     6361 gatcaagatc atgccctgtc tggacatgaa gaatggacgg gtggtgaagg gcgtgcactt
     6421 tgtggacatc aaggatgcgg gcgatccggt gggaaacgcc gccttctacg agagggaagg
     6481 ggcggacgag ctggcgatgc tggatatcgc cgcgaccctg gagaaccgcc ccacgcgcct
     6541 ggaatgggtc cggcaggtgg cggcggtgat cggcatcccg ctgacggtcg ggggcggcat
     6601 caacagcgtc gaggatatcg aaatggtgct ggcggccggg gcgggcaagg tctccatgaa
     6661 cagcgccgcc gtcaagaatc cggagctggt cgcggaggcc gcgcggcgtt tcgggaccga
     6721 ccggatcacg atcgccgtcg acgccaggcg caatgccgcg atgccgtccg gattcgaatt
     6781 ggtcgtggcc gggggaacga cacccgtggg acaggacgcc gtcgcctggg cgggccggtg
     6841 tgaagacctg ggcgccgggg tcatcctccc cacgagcatg gacggcgacg ggaccctggc
     6901 cgggtacgac ctcgaattca cccgcgcgat cgccgaccgc gtgaccgttc ccgtcgttgc
     6961 ctccggcggg gcgggaaccc tggaacattt ctacgagggc gtcgttacgg gcggggcgca
     7021 aatcctcctg gcggcgtccg tgttccattt ccggaccttc cgcatccggg aggtcaagga
     7081 ctatctgcgc ggcaggggac tgcccgtcat ctgaagatcg gaagacagca aggaaggagg
     7141 agcatcagca tggaattcaa gagttgcagg gccatcgtca cgggaggggc ctcgggcctc
     7201 ggggaggcct gtgtccggcg cctggcggcc gccggggcga aggccgccat cttcgacgtg
     7261 gccgtcgagc ggagcgagaa actggccgcc gagctgggtc cgaacgtgat cttcgcccag
     7321 gcgaacatca cgagcgacga gagcgcccag gccgcggtcg gaaaggtcgt ggacgccttc
     7381 ggcgggatca acgtcgccat caactgcgcc ggtgtcggcg atcccggaaa ggttctatcg
     7441 aaaaggggcc ccctttccct ggcggccttc aaccgcgtcg tccagatcaa tctggtcgga
     7501 accctgaaca tcattcgcct cgccgtcgaa cagatggtga aaaatgaacc caacgccgac
     7561 ggggagaagg gcgtcatcat caatacggcc tcgatcgccg cgtttgacgg tcagatcggc
     7621 caggccgcct atgccgcttc gaaggcgggc gttgtcggca tgaccctgcc cattgcacgg
     7681 gaatgcgccg aatacggcgt ccgcgtcgtc acgatcgccc cggggctctt cgacacgccg
     7741 atgctggccg gtctgccgga tccggtccgg gatatcctcg ccagtacgat tccttttcca
     7801 catcgtctgg gccggcctga ggagtacgcc tggctcgcgc agcacatcat cgagaacacg
     7861 atgctgaacg gcgagtgcat ccgtctggac ggcgccctcc ggatgcaggc gaagtgacgc
     7921 tcaggccccc gcggcggcgg ggtggtggct gcggcctcgc ccggggcgag gcgatcatcc
     7981 tcaaggcgca gggccgggcc tcgcgggccg acagaaattt tgtgagaaag gaggacggaa
     8041 gtgtttctcg tccctgagcc ttcccgtgcc ggggattgac agcgtaaggt ttgtgttccc
     8101 gaagaggacc ggggcgttca tctgcgtcgg cgtcatgcgt gtcgaaaaaa ttcgttgttc
     8161 aatcaattct caaggaggga atgacaatga ttttggcgtc acaggaaatg atcaagaagt
     8221 ggacggaagc gggcgcgtgg ggggacaaga cattcattga ttacttcaag gagcacgtcc
     8281 gtacccagcc ggacaaggtc tgcctcgtgg atcccatgaa caaggaggcc ctagtcggcc
     8341 tcaaaccgga acggctgacc tacaaggaac tcgaccgggc cgtggacgcg accgccgagg
     8401 ccttcgttaa cctgggcatc aagaaggacg acatcatcat ggtgcagctc ccgaactgct
     8461 gggaactcgc catgctgtac ctggcgatca cccgggccgg cgccctgatt tcacccatgc
     8521 ccatgcagtg gcgggcgtcc gagcatgact tcatcgccga aatgaccgag gccgcggccg
     8581 tcatcacggt cgaggaattc ggcggcttca agcacaagga gatggtcgag aagatccagg
     8641 cgaagcgccg ctcgatcaag cacatcctga ccctgcccca gatccgggag atgacgcagg
     8701 gcgaggttac cggaaaactc gatcagatcc acatcgatcc caacgacgtg ttcaccctct
     8761 gctggtcgtc gggcacggag gcccagccca agggctgccc gctcagccac aacaactggc
     8821 tcttccagac caccctgtgt tacgacagcg ccccgatcct gccgggcgac aacttgatca
     8881 ccgccgggcc gctcgtgaac atggcctcca tcggaacggt cttcgtcgag tggctgaggg
     8941 gcggcgggaa gttcgtcctc catcacccct tcgacggggc gaccttcgtg atgcagatga
     9001 tcctggagga gatcaactac acgctgctcg tcccggccat cgtcaatgcg ctccttaagc
     9061 atcccaaggt ggaccagttc gacttctcca aggtccggtc catcaccatc ggggcggcgc
     9121 cgccgtccct gtggtccgtc caggaactga agcgccgctg ggggatcgag ttcggcaaca
     9181 tctggggcca gaacgaaggg acgggcaaca tcgccggcgc caagcacatc accgacatgg
     9241 agatgcgcat cgatcacttc ccgctgctgg gaacaccggg cacgccctgg tacgagggcc
     9301 aactcgcctt tgtcaccatg aagctgatcg acccggtgac gaagcaggaa gtcacggaag
     9361 aggggaaggt gggagagctg tggtaccgtg ggcccaacgt cattcccggc tacttcaagc
     9421 ggccggacct gacggagaag tccttcgaca aggacggctt cttcaacacg ggcgacctct
     9481 tccagatgaa ggacaacaac tgcgtcggct tcttcgaacg gtccaaggac atcatcatcc
     9541 ggggcggctt caacgtgagc gcccaggagg tggagaacct ggtgctgggc tatcccaagg
     9601 taacggacgc ggcggccgtg gccatgccgg acgagatcct gggcgagcgg acctgcatct
     9661 acgtcgtgcc gaaaccgggc gagacgatca ccctcgagga aattgtggcc ttcatgaagg
     9721 agaagggtct ggccgtctac aagattccgg agcgcctgga gatcatcgac gccatcccgc
     9781 gcaatcccgt gggaaagatc atgaagaagg tcctccggga ggacatcaaa cagaagatga
     9841 agaccgcgta agcggcgtga agacgccggg cggtgccccg acgggccgcc cggcatccat
     9901 gggcgggaag gagatccatg cgggggaata tcgatagcgt gggcgacatg ttcgccctga
     9961 cggcggcgaa atatccggac aagacggccc tcgtctttca ggacgagcgc ttcacctacg
    10021 gcgaattgaa cgcccgggtg aaccgcctcg cccgggccct gacggccgcg ggcgttcgca
    10081 agggagacaa ggtggcgttc ctcttcatga acagcccgga gttcgtcgtc acatggctcg
    10141 ccgtcgccaa ggccggggcc gtcggcgttc ccctgaacta cggcctcgtt ccccgtgaac
    10201 tcaaatatca gatcggccag gccgacgccg tggcgatcgt cttttccccg ctcttcatgg
    10261 agcggatcgc ggcgatccgg gacgacctgc cccaggtccg cacctatctc tgcaacggcg
    10321 agaacggcac cctggatttc gaaaccctgc cgccggctac ccgcccgatg agccggaggt
    10381 ggacgtctcc ctgtatgacg ccaacctgat cctctatacg gccgggacaa cgggcgtgcc
    10441 caagggggcc gtcctgaccc accgcaacag cctcttcaac gccttcacct gctgtctcga
    10501 ctaccgcttc acatgtgacg acgtgctcca gatcgtccca cccctgttcc atgccgcggc
    10561 cgtcaactgc atgctcctgc ccggcctggt cctgggatgc acgattgcga tccacctgcg
    10621 cttcgacccg gaggacatgc tccggacgat ccagcaggag gccgtgacgg tgacctgggg
    10681 tccggcgatc ctctggcgga cacttttggc ttatcccgac ttcgcgaagt acgacgtccg
    10741 gtccgtgcgt ctgctgggga acggcgggat ggcgatgccg ggcgagatgc gcgagaagct
    10801 gctcgcccat ttcccgtgcg ccgcgatgat ggacatctac ggcatgacgg agctctcgcc
    10861 cgcggcaacg ctgctccggc cggaggcggc gctggcgaag ccgctctccg tcggcacgcc
    10921 catgaccctg acggacgtcc ggattttcga ctggcgcggc cgggaggccg ccgtcgggga
    10981 ggtcggcgag atcgccgtcc gggggaacgc cctcgacggg tattacaaaa tgcctgagga
    11041 aaccgggcag gccgtcaagg gcggattctt ccgcaccggg gacctgggca aacgggatga
    11101 agacggattc atcaccctcg tggaccgcaa gcgggacgtg atcaccacgg ggggcgaaca
    11161 ggtgtattcc cgcgaggtgg aggaggtcct gcaggccaac ccccgcatca aggaatccgc
    11221 cgtgatcggc gtggcggacg agaaatgggg ggagcgggtc gtggcgatcg tttccctccg
    11281 tcaggagacg acgccggacg ccctcctcgc ctggtgccgc gagaacctcg ccgcctacaa
    11341 gtgcccgcgc cagatccaca tcgtaaacga catcccccgc aacgccgccg gcaaggtcgt
    11401 caagggcgaa ctgagaaaac gcttcggctg atgcgaagca accggcctgc cggagaaggt
    11461 cgagagaaca tgaaccggcc cttctaccag gtcgaagcct ccaccgcgga ggtcttctcc
    11521 ggaaatcccg ccgccgtgtg cctgctggag gactggctcg aagacgcccc gctccaggcc
    11581 gtggcggccg ataacaacct ctccgaaacg gcgttcctgg tgcctaattc ggacgggttc
    11641 gatctgcgct ggttcacgcc gaccacggag cggaggtggc gctctgcggc catgcgaccc
    11701 tggcgagcgc gtgaaaattt cgggccgagc ggccctctat ctggaagggc cgatcactct
    11761 taatacgcta ggacgcgccg agcgcggagg acgtcttcaa gcgcccgcgt gacggagcga
    11821 gggggcgttg tccctcttcg ccatggtgtg aaaaaagggg ttcaatcgac gggcgttccg
    11881 tcatccgacg gattcccgaa cacaaaggag gtgcggcatg agtcagattt tcaatttgcg
    11941 cgcggaggaa gggatcggca tcgttacgtt cgatatcgtc ggcgatgcaa tgaacacctg
    12001 gacggacgcg gccttcgagg atttcgaccg gctgatgggc gacgtggaga aggagaaggg
    12061 cctcaagggg ctcatcttca tctccggaaa gccggagaat ttcctgtccg gagcgaactt
    12121 gaagacgatg gcccaggtgg agtccgccga cgtggcccgg acgatcctga acacgtttca
    12181 cggctgcttc acccgcatgg gccggctggg ctatccggtc atcgcggcga tccacggcca
    12241 ctgcctgggc ggcggcctgg aattcgccct ggcgtgcacg gcgcggatcg ccaaggaagg
    12301 aaagaccacg atcatcggcc tgcccgagtg caacgtcggc cttttccccg gcggcggcgg
    12361 gacgcagcgg ctgccccggc tgatcggcta cccggccttt gaactgatcc tcaaggggaa
    12421 gatgctcccc gcggtcaagg ccctcgaact ggggatcatc gaccggatca tccccgccga
    12481 ccaggacctc ctggcggcgg ccaaggcgtt cctcaccgag atcaatgccg ggaaggcgga
    12541 actcaagcgt ctggagcagg atttctccca ggtggatgcg atcgcggaga tggccaaaca
    12601 ggggatcctg aaggccacca agggccgcga gatccccggc ccgatgctgg ccctcgactc
    12661 catgcaccgg ggcgtcaagc tctcgctgga ggaggggcta gaactggaga aggaaaactt
    12721 cgtcaaggtc atcctgagca accaggccaa gggcagcatc aacaccttct tcatcaaggg
    12781 gatgacggac aagcccaaga gcatgatgac aaagggcttc gttcccaagg cgatcaacaa
    12841 ggccgccgtc ctcggattcg gcacgatggg ccgcgggatc gtcatcgata tcctgcgcaa
    12901 cacgaagatc cccgtcatcg tgaaggacgt ccccgaggcc ctggaaccgg gcaaggcctt
    12961 cctccggaag atcctggacg gcatggccga gaagaagcgc ctcaaggagt ccgtggagga
    13021 cctgatggcc cgcctgacgg cgacctcgga ctacatcgac gccttccgcg aggtggacat
    13081 cgtcatcgag gccgtcttcg aggagatcgg cgtcaagaag caggtgtacg aagaactggg
    13141 caaggtgatc cgagaggact gcatcgtggc gagcaacacc tcctcgattc ccatcacctc
    13201 catggcgccg ttcgtcgtga atccggcgcg gttcggcggt attcacttct tctctcccgt
    13261 ctggatgatg cagctcgtcg aggtcatccg cggcgaacag accgcccagg acacgatcga
    13321 caacctgctg aacttcaccg ggctcatccg gaagcggccg atcgtctgcc gcgacaatcc
    13381 gggcttcgtc gtcaacgcgc tgctcttccc ctacctcaag ctggcgctgg agtacatcga
    13441 ggcgggcaat cccatcgaga aggtggacgg ggccgtaacg gccttcggca tgccggtggg
    13501 gccgatccgt ctgacggacg aggtcgggat cgacgtgccc tacaaggcca tgaagggcat
    13561 gggattcgtt cagccgacgc tcaagaacgt cgtggaggcg gggcgctgcg gcctgaagaa
    13621 atccggcaag ggcttctttc tcaaggacgg cagcgtcgat cccgaggtcc tgcccctgat
    13681 cgcgaaggac gcgccgaggg aggtctcggt cgaggagatg cagaccgggt tcatcgaatc
    13741 catgctgaag gtcggcaagg acctcctgga ccgacagatc gtgatgtcgc ccgccatgat
    13801 cgatgtcggg atgatctggg ggaccggctt cccgccggac aagggcggcc ccatgaagtg
    13861 gggcgacctg acggggctga gcgagaaact cttcgggaag aagttttacg cgtagcgggc
    13921 gggatcaggg cgccgggggc aatgtccccc ggttccgccc tgatgcctcg atcaggccct
    13981 tcaggtgcgt ttctccgaag aggatcaggt cccgcatggc ggcgaccgca gcctcggggt
    14041 cctttgcgtc cacggccgtg gcgatccgcc ggtggcagga ggagactcgg ggcacgaccg
    14101 gggcgtcgat gaaggactgc cgggccatgc tgagatagac gttcttgaag gaattcagga
    14161 gcaacgggta gaagaggttg ttcgacgcca tggccacatg gtggtggaag cggaaataga
    14221 ggtccgccgt ggcttcgagg tccttcatgt cgacggtgtc ccactcgccg atgagacggt
    14281 gaaagacctg gcgctgctct tccgtgcggt tcagggcggc cagacggacg gtctccgtct
    14341 cgaagaggat gcgcatctcg aacatactgt ccaaaagctt cgtttccagc tctcccgaat
    14401 aatgactcag caggctggag aggatcgcca gagatccgtc gagccggtaa tcgttgacca
    14461 cggtcccgac gcggggcttc atcgtcacca tccctcggac ctccaggtcc cgcagtcctt
    14521 cgtggacgac gggacggctc acgcccaagg cttcggcgag ttcacgctcc gaaggcagtt
    14581 tctggcccac ctccagcttg ccggacagga tgagcgcctc gaagcgctcg atgaagacct
    14641 cttttaagct cttggcccga atcggggcca gcatctgatc catggacgac cctgcacgtc
    14701 acgaaaccgt cgatccggaa ggccattgtc gcctcccgtg cacgttctca tatcacaagt
    14761 ccgcgatgat ttccctctga aaagcatcgc ccggcggacg gcctcctcgt ccaccgggcg
    14821 gcggcgcacg ctcatccctt gcgccggacc agtgttgtca ggaaccccca ccaggccttg
    14881 acgtccgtca ggtacgcgac gcgctcggag atgatcgcca cggatttgac cgttttgcga
    14941 agctcccaaa gcgtcatgga aagcaatacc agaacgacga tcgacatcaa cgccaccccg
    15001 atccataaga acggcaaaat ctgttcggcc ataacgttct cctcccttct gatgagagag
    15061 ggggccgccg tgaggcggga cgccgcggcg gcccccccgg gtgcctgttc gatcgatcct
    15121 agaccatgta ccgacccggc agggacgggg cctgcttcag gaggaagcgc agctgctgtt
    15181 tccggtgcgg gggaaggaag cggtagaata cgatccccag tatggcgacg acgatcacgg
    15241 acaggaagaa tttctttttc ttggtcatgg gtgctctcct ttcttggggg tttagcatcg
    15301 ttcgtgtttc aggatctggc tgtaggcgat gtaaccggag gacatggtcg tgggaacgcc
    15361 gccgccgggg tgggtccccg accccgtgag gtagaggcca tcgatgcatt tcgatttgga
    15421 cgagggtcga aagactgcct ggtgtgaggc gtcctggtgg aagcagtaga tggacccctc
    15481 gggaaggcgc aggtggcgtt cgaaatccac cggggaggac ccgatggcat gggtgatgtg
    15541 tcccttcagg tccgggacca gtttttcgga gaggtagtcg atgtattcgt cgatgaaccg
    15601 gtccttgaag gcgtcccagt tcgtcccctt cagatggtag aaggattccg ggatcacgtt
    15661 caggacgtga tggccttccg gggccagcga gtcgtccgag tgcgacggcc agcagacgag
    15721 gccgtaattt gcattcgccg gcagcatgac ccccgtctcc acatgctttg cccagaagtc
    15781 gttcacctcc tgcatagaga cggcgatgta gctgtggtgc gcgtccagcg gcggcttgca
    15841 gtccacgccg acgtaaacca tgcacaggga cttgccgtac ttgaggctct tgacgccggt
    15901 ccgcgtcagc cacggcaggt gctgttcgcc gatgaggtcc aggtagagca tcctggggtt
    15961 cacgttggag acgaccatcc gggagctgat ctccctgccg tcttccagga cgacgccctt
    16021 cgcgcggccg ttgcggacga tcacgctcgt cacgggcgtg ttcagccgga gttccatccc
    16081 gtccttctgc ccgcaacgga cgagggcctc gggaatgcgg atcatgccgc ctcgcggata
    16141 ccagatgccg cggtgctcgc tgtaggggat catggcgaac ggtccggggc aaagggccgg
    16201 cggcatgccc atgtagagcg actggtaggc catggtccgc tggacctggt cgctcttgaa
    16261 gtacttctgg atcaggtcct gataggtgga catgaagttc ggaagatagc ggagcagggc
    16321 cgggttcttc ttgaccaccc gagccatgtc gcccagggtg tcggctggct ccaccaggat
    16381 gctgtccagc agtacgtcca tcatgtcgct gcagtaatcg cagaagtcga accagcgcct
    16441 tccgtctgcc gggtccaggg aggcgatcag ttttccggtt gcttcgatgg atttttccac
    16501 cgtgatgcgc tcgccgttgc gatacatcac cgaaaagatc ggatcgcagg gaatcagatc
    16561 gacctcttcg ccgaagctgg ttccgagggc cttgaaaacc agttcgatgg gctgcgtgat
    16621 ttccacgatg gacgccccga cgtcatgccg gtacccgtcc cgctcgaacg tggaacagca
    16681 accgccgatg cggccgctct gctccaggac cagaaccttg agcccccgat gggccagcag
    16741 cgcccccgac gagaggccgc ccagtcccgc cccgatcacg ataacgtcat agtctgccat
    16801 aagtctgctc tcctttccga tgcgatcttg cacggatctt tgcccgtgcg ctgttcgtga
    16861 tcgtccggcc gcttccggcg aaccacgctc cggcgatccg aaggggtcgc gggcggcgga
    16921 cgcccgggcc tatgcaaagt accgcgggat gaggtacggc acttgacgca ggatctccct
    16981 gaggaagcgc ttcttgcgct ccggctgctt cacgtagacg tacacggcga cagccgcacc
    17041 cgccaacagc agaatcttct tgatgttcat gggtctcctc ctactcgtag cgttggatga
    17101 ggtcggccgc gatcatgccc gaggcgatgg tcgtggggac gccgccgccc ggatgcgtcg
    17161 aggcgccgac gaggtagagt ccggcgatgc ttttggattt ggcggcgggg cggaacgtga
    17221 cgccgctcgt caggtcctgg cgcagggcgt agatggcccc ctcgggtgag aggagccggc
    17281 gctcgaagtc caccggcgtc gacagttcgg cgacccggac gtgctccgcc aggccgggaa
    17341 tgtaccgctt cgacatgtag tcgaggatct gttccgtcag ggcggccttg aagtcgtccc
    17401 atgtggtgcc gtggagacgg tacgggccgg gttcgagggt caggacgagg acgtggtggc
    17461 cctcctgcgg ggccaggccg gggtcggagt gcgaggtcca actgacgatg ccgaaatgct
    17521 cttcggcata gcgatccttc ttgtagacgt tccaccagta gtcgttcagc tcatccatcg
    17581 gacggctgat gagggtgtga tgtccctcga ggggcggggt gtagtcaacg ccgagataga
    17641 gcatcggcgt cgccatggaa tattcgtagc tcttgatgcc cacgcgtgca agccagggca
    17701 ggtgttcctc tccgatcatc ttcaggtaca ggagcttcgc gttgatgtcc gagacgacca
    17761 ggtcggatgt gatctccgtg ccgtcggcaa gggccacgcc gagggcgcgc cggttccgga
    17821 cgatcacgcg gctcacccgg gtcttcagcc ggacggtcag gccgaaattc tgcccgaggc
    17881 gttcgagggc gcgcgggatg ccgatcatcc cccccttgct gtaatagatg ccggcgtgct
    17941 cggaccaggg gatcatggcg atgtagcccg ggcagctgtc cgggggcagg ccgccgtaaa
    18001 agctctgaaa ggcgatggat tcgcggatcc tcgtatcctg gaaatacttc tccatcacgc
    18061 cctggtagct gctcgcgaac atggggccgt acttgagcag tttcggggtc ttggcgaaaa
    18121 ggttcacgag gtcgccctgg gtgttcgccg gcgcgaggaa gaagcctttc agggcggcgt
    18181 cgaggaatcc ctgcatgtaa tcggcgtagg cgtgccagcc cttgacgtcc tcgggcgcga
    18241 gggcgccgat gttccgggcc gaagcctcgg ccgacagggg atatttgagt cgggtgccgt
    18301 ccttcatgat cacgtcgtag acgggatcgc aactgaccag atccacctcg cggtcaaggg
    18361 tcgtgccgag acgctggaag caccagtcga tcacctccgc gtcctcgatc agggatgcgc
    18421 ccagatcgaa cgtgtacccc tccttttgga aggtggagca gcacccgccg atccggtcgc
    18481 tctgttccag gacgagcacc ttgcggccct gcttggcgag cagggcgccc acgctgaggc
    18541 cgccgcagcc cgcgccgatg acgatgacgt cgtaatctgt catggaatcg cctcctgtcg
    18601 attattccac ccgctttgaa acccactctc ccggaggcat ccggctctgc cgggagagcc
    18661 agcggagcaa caagcgcccc aacctggtac taaagcagat ggtattacca ggtgccaaat
    18721 acgggaagat tgccaggatg tcaagagaaa tccaggggaa aggccgctaa ccccattttt
    18781 cttgacttcg cggtatttta aggtaaagtc ataacccgtt gagtttcttt cgaatgaacg
    18841 attggtcacg catgaaatgg cccgcttgac gacaatgaag gcccagaagc ggcctcgggc
    18901 cgcttttcct tgacatccga acgctggtac gccgtagccg gacacgataa ttcggaacgg
    18961 ccgccacccg gaacgcagac gacggattac acgggaaggc cgatgccggg ggagcaagga
    19021 cgcggaccgg ttcgccggtc gagtacggaa aggacgcaat gtcgagccca cgcaagaaac
    19081 agattcgaaa tatcgccatc atttcccatg tcgatcacgg caagaccacg ctggtcgatg
    19141 ccatgctccg gcagaccggc accttccgga tccatcaggt cgtggaggag cgggtgatgg
    19201 attccctgga tttggagaag gagcgcggca tcaccattac cgcgaagaac acggccatcc
    19261 ggtacgggga ggtgaagatc aatatcgtcg atacccccgg acacgccgat ttcggcggcg
    19321 aggtggagcg gagcctcaac atggtcgacg gggcgatcct cctggtcgat gccagcgaag
    19381 ggccgctgcc ccagacccgc ttcgtcgtga agaaggccct ggccgcgaaa ctccccatca
    19441 tcctcgtcat caacaagatc gaccggtcgg acgcgcggat cgaagcggtc ttgaacgagg
    19501 tctacgatct cttcatcgac ctggacgccg acgaggcgca gatcgaattt gccgtcctgt
    19561 acaccaacgc caaggccggt gtcgcccatg aggtgcaggg ggatggttcc gccgatttca
    19621 agcccctgtt cgagacgatc ctcaagcgca tccccggtcc ggaaggcgac gacggggccg
    19681 ccccgcagtt ccttgtcacc aatctggact acgaccccta tgtggggcaa ctggcgatcg
    19741 ggcgactcat gaacgggacg ctggagatca accagggcta taccctctgc ggcgagcacg
    19801 aagacacgcc aggggtccgc ttctccgccc tctatacctt ccacggcctg cagcgcaaag
    19861 ccgtggagcg ggtcgaagcc ggggacatcg ccgcctttgc cggcgtggag ggcgtcaaca
    19921 tcggcgatac ggtggcggcc ctcgtgaccc cgcggccgct gccgcgcatc cgtgtcgatg
    19981 agccgaccgt atcgatgatc ttctatgtga acaatggtcc gttcgccggc aaggagggga
    20041 agtatctcac ctcgcgccac ctgcgggacc ggctggagag ggagacgttg aaaaacgtgg
    20101 cactccgctt ccagcccctg gagcgcaagg acgccttcga gatctgcggc cgcggagaac
    20161 tgcagatggc ggtactcatc gaaacgatgc gccgggaagg ctatgaatgc atggtgtccc
    20221 gtccccgggt catcacccgg gaagagaatg gcaagaccct ggagcccgtg gagcgtttgt
    20281 tcatcgacgt tcccgaggtg tacatcggca ttctgacgga gaagctggcc gcgcgaaagg
    20341 ggcggatgac cctgctcgtg aaccggggca gcggccgggt gaacctggag ttcatcatcc
    20401 cctcccgggg gctcatcggg ttccgaagcc atttcatgac ggacaccaag ggctccgggg
    20461 tgatgaacac cctcttcgag gggtacgagc cctgggcggg acccataccg cagcgggtga
    20521 gcggcgccct cgtggcggac cgcgtcggcc gggtgaatac gtatgccagt ctcgccatgg
    20581 cggatcgcgg cgagatgttc gtgcccgtgg ggaccgaggt ttacggcggg atgatcgtcg
    20641 gggagcgcaa ccgcacgggc gatctcaacg tcaacatcgt caaagagaag aaactgacga
    20701 atatgcgcag ctccacctcc gaggcgaccg tgactctgcg tccgccgcgc ctcctgtccc
    20761 tggaccagtc catcgaattc atcgccgagg acgagctggt ggaggtgacg ccgcatagca
    20821 ttcgtcttcg gaagctcgac ctggttgcct acacgcgcac ggtccggtcc tgaggggttt
    20881 gccgcgcgat gcgcctccgg gagcggattc ccgggccggg cgggggtgca gcgtgacggt
    20941 ttgcccggga gcgatgcccg atgtgcggga agggcaaatg catgcattaa agaggtgtga
    21001 cgatgacgta cgtgatgggt ttggatgcgg ggggctccgg cggccgctgt ctgctgtggg
    21061 aggtcgggag cgggagggtg tttagtgcga gcgcgggctg gtcccatccg gtcgcgccgg
    21121 ggacggccgg tcttggattc aatctcgacc tggacgacgt ctgggcgaag ctgggccggg
    21181 cggtccgtga ggtcctctcc cgctcgggcg cgacgccgga ggacgtgctg gcgatctccg
    21241 cgaccagcat gcgtcacacg acggtggtgc tggatgccgc gggggatgtc ctgctggccg
    21301 ctcccaatca cgacgcccgt gccctgggtg agggtcttat cctgggcgcg gaacacggcg
    21361 gccgcgttca cggcatcggg gggcactggc cgagtccgat cttcaccggg gcgcgcctcc
    21421 tctggatgaa agccgccgcg ccggaggcct tcggccgggc ggcccgcgtc ctcaccctca
    21481 gcgactggct ggcgtaccgc ctgagcggcg tggtggccgc cgagcgctcc caggccgggg
    21541 aaaccctgct gttcgaccag gtccggcgcg aatgggcgga ggacctgatc cacgacctcg
    21601 ggctgccggc ggaggtcttc cccgcgctcg tggacgccgg gacccgtctg ggcggtctga
    21661 cggcggcggc ggccctgcac ctgggacttg cggaaggcac gccggtggtc gcgggcggtg
    21721 cggacaccca gtgcggcctc ctcggcgccg gcgccgtgcg ggccggcgat ctgggcgtca
    21781 tcgccggcac cagcacgccg ctgcagatcg tgactgcccg accggtgttc gatccggagg
    21841 gccggctctg gaacgggcag cacgtcatcc ccggactgta tgtgacggag agcaacggta
    21901 tggcgacggg cgaggtgctg gcgtgggtcg ccgccatgat ctatcccgat tgcgcccgcc
    21961 cgatggaggc cctgcttgcc gacgcggccc ggtcgacgcc gggggcggag ggggtctcct
    22021 cgaccctggg ggcccatatc ttcgacggcc ggaccatcgg aatccccgtt gggaacctga
    22081 ccctgtcaca tatggcgagc cccgggagca gtcggcggga actgggccgg gcggtccttg
    22141 agggcctggc cttttccgtc gcggccaatc tgaagcagat cgaggccgtg accggtcagg
    22201 cggtcccaac cctgaagatc accggcggcc tgtcccgcag cccgctctgg acggagatcg
    22261 tgagcgacgt gaccggccgg gcggttcagg tgcccgcgac ccacgagtct tcctcattgg
    22321 gggcggccgt ttgcgccgcg gtcggtgttg gcgcctttgc gtcgttcggt gcggcagccg
    22381 cccggatggt ttcgatcgcg cgcgagcagg cgcccggcgg ggatgccgaa tcctaccgaa
    22441 agctctatgc cggctggaag gaggcccacg gcctgcgcgc ggcggccgac agccacatgg
    22501 ccaatctgct gacgacggcg atgatggagc gcccggtttc ccgggcaaaa cccgcgcccg
    22561 gcttccggcc ccggatcaag gtcaccgcct cgctcgacga catggttctg gaccggctcc
    22621 ggcagtacgg cgaagtcgac tacgccaact ggcgggagac ggcaaaggtc tacagcggcg
    22681 aagacgtgat cggcctgctg aagggatttc atgccttcat caccgagatg gacgtggtgg
    22741 cgctggacgc cgtccgtgag cttccggatc tccggttcat cgccacgtgc cggggcaatc
    22801 cggtgaacgt ggacgtcgag gcctgccggg cgttcgggat tcccgtggtc tacacccccg
    22861 gccgcaatgc cgacgccgtg gcggacctgg tgatcgcgtt catgctgatg atggcgcgca
    22921 ggctgcccga ggcgagcgcg ttcctgaagc tgccgggtgg gaaggccggc gacctcaacc
    22981 ggatggccga ggcctacctg aactaccagg ggcgggaact ctggggaaag accgtggggc
    23041 tggtggggtt gggccaggtg ggcgccgggg tcgccgcgcg gctccggccg ttcggggccc
    23101 gtgtcctgta ttacgatccc ttcgtcgcgg acggcgccct ctttagcgcc gtccggacgg
    23161 gcctggaaga actgctcgcc gagagcgact tcgtcagcct gcacgtgccg ttgagcgacg
    23221 cgacgcgcgg ccttctggac gcggccgccc tggagcggat gaagccgggg gcttgcctcg
    23281 tcaatacggc gcgggcgggg ctcgtggacg aggccgccct gtcccgggtc ctggaatccg
    23341 gccggctcgc cggtgcggcg ctggacgtct tcagcgtgga gccgccggcg gcggacaacc
    23401 ccctggtggc cagtccccgg gttatcgtga ccccgcacct gggcggcaac accgtcgagg
    23461 tcggcacgca tcagggcatc atcgccgccg aacagctcga gcagctcctg cggggcgaga
    23521 ccccggaatt cattctgaac cgggaggtca tgacggcctt cagctggacc ggaccccggc
    23581 ccgaacccgg ggtggaggag ctggcccggg tcgcccaggg ccccggaccc acgatgacgt
    23641 cgtagggcag ggcaggggat gtgctcgtaa gggcgatggg gcgttgaccg atctcagatc
    23701 gggagagagg cgaccagccc ccgggagatc ctgatccccg cacgcaaagg gtcaggacct
    23761 ccggggctgt cggggacttt caggatttca ataggccctt gatttcctcc ttcagggcga
    23821 gcttgctcac cttgccgctg gccaacagcg gcaggagggg gcggacaaag aacttcttcg
    23881 ggaccttgaa gttcgcgagc ttggagcggc acagctcgcg cagttcttcc tccgtgactt
    23941 ctttgcccgg cgagggcatg acgaaggccc acccgacctc ctggtagatc tcgtcgggaa
    24001 cgcccatgac ggcggcgaac agtacgctgg ggtgcgtctc gatgatctcc tcgatctccc
    24061 ggggatacac gttctcgccg ccggtcttga acatctccga cttgcgcccg gtgatcgtga
    24121 tgtagccccg ggcatccttg aacgccaggt cggaggtgta gtaccagccg ttccggtcca
    24181 ggacctccgc cgtggcctcg gaattgttca gatacccctt gaacatgaac ggaccccgga
    24241 cggcgatctc gccgatctga ccgtccggca gctcccgccg gtcgtcgtcg acgatcttca
    24301 gctcgaaggg cggcgcgatc ttgcccgcgg tcttgagcag ggtgttcgga tcgtcgccct
    24361 tctccgtata ggtgacgaac ccgcagacct ccgtgctccc gtaacccgtg atcatcatcc
    24421 cgcccgtctt cgcgcagatc cccgacagga cgtcgatcat gatcttcggg gcggccgcgc
    24481 cggcccaggc catgagccgg acgtggctga agtcggtctg gaagaactcc ggctgcttca
    24541 tctggaggag gaacatcacc ggcacctggc cgaacatggt gatcgtttcc ttttcgatgg
    24601 ttttgagcgc gccgaccgga tcgaactgct ccatgaagac gaggcacccg ccggccatga
    24661 cggcggcgaa gccgatctcc acgtccgcgg ccacgtggtt gatcgggaag tgcagcaggc
    24721 agcgcgtctc ctcatcgacg tagaacttga cgacctccac tttgatgttc tccacgatgc
    24781 tcccgtgggt atggacgacg cccttgggtt tgccggtgga gccggaggtg tacatgagca
    24841 gggcatcgtc gccgggccgg atgtccgccg cgcgtttttc catggcggcg tcgagttccg
    24901 ggcggggccg gtcgatgaag tcctggaaat tctcggtgcc ttccaccgga tcgccgatga
    24961 cgaggactcg cttgatgaag gggaattcct ctttcagggc cgcgatcgtt gcggccaggt
    25021 cgttgccgag gaaggtgcgc acggcgatca ggacgaccgg tttggagtcg ccgatctggt
    25081 agcggagctc gtcgagggtg aatttgggat tgaggcccag ccacatcccc ccgactttac
    25141 ccgccgccat gtacgtcatg aggaattcgt tgcgcgccgc ggacaggagc gcgatccggt
    25201 cgcccttctc caccccgatc gcgagataag cccgtgcgat ccggtccatg cgatccttga
    25261 agtctttcca ggacaggcgt tcgtcgccga agacgagcgc ctccgccccc ggtttctcct
    25321 gtgcccactt ctcaacgtag tgccaagtca gattcaaatc ctgccagccc atggttgcct
    25381 ccttgtcgct tggatggtgt cggaacggat ccggcgatcc gttttcctgc tgcgatggaa
    25441 tgacccggcg gtttctcctt ccctcatcag cgcaagaggg tcacgacgcc gtcgctgacg
    25501 gcccttttcc cctcggtgtt caggatggag aagaacaggt gcgtccggtc gccgtcgctc
    25561 cggctctcca gcaaccgcac gcggatctcg ctgccgggga tgaccatccc cgtgaagcgg
    25621 caggcgatcg atcggacgcg ttccgggtcg ccgcctgcct cccgcttgat gacgtcccgc
    25681 acccccaggg cgagggtcgc cgtcccctgg tagataatgt ccggcagccc cacctcgcgg
    25741 gcaaatttca cggacgtgtg gatggggaag tggatgttcg tgcagccgtc gtacacgaag
    25801 ggcgccgtcc gctcgatgcg gatccgcgtc tcccagaggg ggtcgcccgg ttcgggttgg
    25861 gggagcgttt cggggagggt ttcggccccg gcaccgtcgt cggtgcagcg gacgccccgc
    25921 atcatgccgc cgatatgctc cgtgaagacg ggggcgccgg cctcatcccg ggcgtcgtag
    25981 cgcagaacca tcagcgtgcc cgctttgtgg gggaggatgg cggcgacccg tccctggacg
    26041 gtcaggcggt cgtccggtcg gatgaggcgg aaaaattcca ggtgctcggt gtagtggacc
    26101 tgggtcgcga ggatttcgaa gggaaagtcc gaagtctcga tgaaatccgg gatgcgttcc
    26161 gagatcggcc aggtgacggc cacggcgaac atgggcgggg cgacaagacc gccgggatcg
    26221 ctgtcgtcga ggtagcgggg attggcgtca tcgagggcgg cggcgtagtt cgtcgtctgg
    26281 cgccaggtga tacgggcgtc cagcggtttc aggggcgagc cgacaaagcg ggaagaaatt
    26341 tccatgacgg atctcctggg tcgggagttt gagaaacgat cgaagggatc ttcgaggcgt
    26401 tttatttcaa atgcacgccg gtgaaatccg acctccaccg ggactccaat taacgctttt
    26461 tttggaacaa tgcaagcgcg tccctcacca gggccggcta tgacgtaacg gttccgacct
    26521 tgtccatggc tgatccatct tgccggttcc ggattttccg gtactggacc agggccgtta
    26581 gggcccgggc agcccgctcc ggtcccgcga atacgggcac gcccttctcc atgagctgtc
    26641 ggcagaatgt ctcctcgatc ccacggtagg tatagccgat caccggccgg ccgtgcgtcc
    26701 cgaccagcgc ggctagggcg ccggtctgcg cctcgatgat ctcgtccgtc tttgcggcga
    26761 tgtcttcggg ggggaggccc atcatctcca gggagcgttg gatcatgagc gacgggacga
    26821 ggatgtacaa aaggagcatg tcggcgccgg gctcccggag gaggacgtcg gggatcgccg
    26881 tgaaataatc cgcctggttc cgggcgaagg tcatgtccac gggattgccg aagctcgccg
    26941 tatgggggag aaacggcttc agccgctcca ccgtcttttc cgacaacagc ggaagatcga
    27001 gcccttcccg tccacaatgg tccgccgcgg ccgctcccgg tccacccgag tgcgtctgga
    27061 tgaccaaccg cctgcccgcc ggcagcggca tcgcgcccag cacccagcag aagtcgaaca
    27121 gctcggtgac cgatcgcgcg cggaggatgt cgcactgtct gaaaataccg tcatagacgg
    27181 cgtcgggacc ggccatggcg ccggtgtggg agaagccggc ccggcttccc gtttcgctgc
    27241 cgcccacgta ataggcgacg atgggcttgc ggggcgcaat ggcgcgggcc gccttcacga
    27301 aggcctctcc ccggcggatc ccctcgatgt acagaccgat gaccttcgtc cggggatcgg
    27361 ccgccagata ttccatgcag tccacgatgt ccaaattggc ctcgttgccg acgctgaagg
    27421 ccgtactgaa gttgatggcg tggcgggtca ggtggttgaa catctgggcg acgaagctgc
    27481 cgctctggga ggccagtccg ataaatccgg gcttctcgtc gtacaggaaa agtgtggtgt
    27541 tgagcctgtg gtgggtgttg acgacgccga tgcagttggg gccgatgaag cggatcccgt
    27601 accgggcggc gacggcgacg acctctttct gccgttccgc gccttccgga ccgacctcct
    27661 ggaatccgcc cgagacaatg accgcccgcc ggatcccctt ttctccgcag gccgcgaggg
    27721 tctccgcgac gagtcgcggc ggcaggacga agagggccat gtccgggact tcgggcaggt
    27781 ccgcgaccga ccggtaggcc ttgaggccct ggacggtgtc ctccgtcggg tggacggggt
    27841 agatcggccc gttgaacccc gtggcgagtt gggcgctcag gatgaggctg cccatggcca
    27901 tcaggttgtt ggaggccccg aagtaggcca tgctgcggca gttgacaatc ggatacaggg
    27961 gactgtcctc gagtgtttgc gtcatcatcc gtctccttgg gattttgaga tgatcggttc
    28021 ttcgccggtc cgggccgttc gggtccatcg aacaggcacg cggagtttag gacgccgccg
    28081 ccgtccggtc aagataaaag ggacagagac ggcaggggaa agatcgcggc gagaagggtc
    28141 cgatccgccc gggattttat gcttgtcatt tggaccgatt ctatgccaga gaaaaccggg
    28201 gcggccgcgt tgtccgggtc gattccgtgc agacccgtcg gcatcggcga cgaaggattc
    28261 attcctcgag cggggaaggg ggcgccttca cacagcccgg ctttgtcttc gggcgtccgg
    28321 acatcgaacg gcttatcaag gaggagattt gatgctggaa cacctttttt cgcccgtgac
    28381 gatcaacggg atgaccctga agaaccgggc ggtcatgccg cccatgggca ccggctacgg
    28441 caacgccgac ggcactgcga gcgaacggct gatgcattat ctcgctgcgc gggcacgcgg
    28501 cggggcggga ctcattatca cggagatctg cgctccggat cctcgcggga aggggtttcc
    28561 caacgagctc ggggcctgga gcgacgacct gatccccagc ctggggaaga tctccgcggc
    28621 cgtccaccgc gagggagcga aggtcgccct ccagctccac cacgcggggc gcgagacctt
    28681 tgccgccgcg gcgggaggcc tgccagaggc gcccagtgcc attcccagcg ccatcctcgg
    28741 ccagccctgc gaggccatga gcctcgcccg gatcgccgag ctcgtcgcgg ccttcgcccg
    28801 ggcggcggcg cgggcaaagg aggcgggctt cgacaccgtg gagctgcacg gcgcccacgg
    28861 ctatctcctg aaccagtttc tgtcgccctt ttccaaccag cgggaggacg cctacggcgg
    28921 gagcgaggaa aaccggtcgc ggttcgtcct ggagaccctg caggccgtcc gggcggcggt
    28981 cgggccggcc tttcccgttc tgatccggat ctccggggac gaattgatcc ggggcggtta
    29041 cgatctcgac ttcacgcggc ggctggcgcc acagctcgtc gcggctggcg cggacgccat
    29101 ccacgtctcc gtcggtgttt attccacgcc ggggaatctg agcatcgcct ccatggatac
    29161 ggcgaccggc ttcaacctct tccgtgcccg ggccatccgg gaagcggcgg gcgttccggt
    29221 cattggtgtc ggccggatcc agcggccggc ggacgccgac cgggcgattg cggcgggcga
    29281 tgcggacctg atcagcttcg gccgtcagca cctcgcggat ccagatttca taagcaagac
    29341 cctcgccggc cgtgaggagg acatccgctg gtgcgttgcc tgtaatcagg gctgcatcga
    29401 gcggctgagc ttcgagatgc ggtccacgac atgcaccttc aatccggagt gcgggcagga
    29461 gtaccggggg gcggccgcgg ccgtcacggg ggcgccgcga ctctgggtga tcggggccgg
    29521 tccggccggc cttgccgcgg ccctggcctc ccgcgagagg ggctttgcgg tcgagatttt
    29581 cgaaaaggcg accgttccgg gcggacagct ccagccggcc agccgcccgc cccacaagca
    29641 gccctttatg gactgggtcc actgggccgt ccgccaactg gaaaagcggg gcgtccgcct
    29701 ccattacaac gaggaaatgc gcgcagaaat cctgcggaga gaccgccctg acgccgtcat
    29761 cctcgcctcc ggcgcctctc ccgtcgttgc gaaaatcccc ggcatagacg gcccgaaggt
    29821 ctgcgacgcc cgcgacgtgc tgatggggaa gatcgcgccg gccgctcccg ccgtcgtgct
    29881 gggggcgggc tacgtgggca tggaaacggc ggactatctc gtcgcgcggg gcatcgacgt
    29941 cgtcctgctc gaaatgcagc cggcccatcc cgttggcaaa ctgacggcgc atgcctactg
    30001 gctgcaccgg cggatccggg aaggccgggg ccggattgtg ctcggtgcca cggtgacgcg
    30061 ggtcgagaag gacgccgtct tctaccgcca gggagacgaa gaacgacggg tgcccgccgc
    30121 cctggtcgtc acggcgatgg gcgcccggcc ggacaacgcc ctggaggggc cgctcgtcca
    30181 actcgggatt tcctatcgcg tcgtcgggga cgccgtcacc ccgcgccggc tcctggaggc
    30241 gatccacgag gggcatcagg ccggacggga cattcaggga tgatcacggc gggtatcgat
    30301 atcgggtcaa tgacgaccaa gggggcgatc gtcgccgacg gtcggattct gatgacgtgc
    30361 gtgcgcttca cggggtacaa cgccgaggcg gcgggggaga aggtgttccg ggaactcctg
    30421 gacgccctgg acctcgaggc gtcccgcgtg gaccgggtgg tggccaccgg ttacgggcgt
    30481 aaggccgccg ccttcgcgca ggcaaccgtc acggagatca cctgccacgc cgccggggcc
    30541 caccacctcg atccccgggt ccgctccgtc atcgacatcg gcggacagga cagtaaggcg
    30601 atcgccctca atccccaggg caaggtccgc gattttgcca tgaacgacaa atgcgccgcc
    30661 ggcaccggcc gcttcctgga ggtcatggca cgggcgatgg aggtcgatct ggacgcgttc
    30721 ggggtgctgt cgctcttgtc ccggaagccg gcgcagatca gcagcacctg tacggtcttt
    30781 gcggagtccg aggtgatttc gctcatcgcg ggcggggaga gccgggaaaa catcgtcgcc
    30841 ggcattcacg aggccgttgc gtcccgcgtc gtggcgatgg cgaggcgcct gggactggtg
    30901 gcgccgatta tgatgacggg cggcgtcgcg aagaacgcgg gggtcgtcaa ggcgctggaa
    30961 aacaagttgg gacacgtcat cgaggtgtcc ccctacgccc aggagaacgg cgcgatcggc
    31021 gcggcccttc tggcggcgtt gcggatttag cgaggtgtct ttccagttcc tgctcgtgcg
    31081 ggattccctc gcgccccccc agccgcgtac tgaccctggc ggcggcccac gccccgagat
    31141 caaggcaggt ttcgggagcg agaccgtgga cgatcccgta ggtgacggcg gcgtggaaca
    31201 cgtccccgca gccggtcgtg tccacggtcc ggacggggta ggcgggcgtt cgaatccagc
    31261 ggccgccgac cagggcgacg tagccttttg cgcccagcgt gacgccgaca aaggggcacc
    31321 ccagcccggc cagacggcga caggtttcca tggggtttcc cgtcagggcc tgagaaaacg
    31381 cctccgaggc gatgaagcag ccgctcaggc cggcgatctc cagcatgccg tcccggagcg
    31441 accccgcgtc gcagacgacg ggaattcccg cctcccgggc ctgcgtgcag gcaaaaaggg
    31501 atgcctcggg gaagaacccg tccgtgtgga gcgcccggct ggtgcgcagc cgctccagat
    31561 cgagttcctc cggggacagc ggcggccccg tcggccgctg ccaaaaaatc gtccggcgtg
    31621 cggtgccggg ctcggccgcg acgaaggcaa attgcgaaag gtggccctcc ctcaccctga
    31681 gcccgcccgt gtcgatcccg gcgtcgcgca tcgaagccgc gatcgcggca ccgaaggcgt
    31741 cgttccccgc gacgcccgcg aaataggccg gcagtcccca gcggcgcaag gcggccaggg
    31801 ccgtggcaac cgggccgccg ccctggattt cgaggttcgt gaattcgcat ttgacgtcgg
    31861 cgggcggata ggccgggatg attccaatgt agtcgagggc gcactgcccg agaccgaaga
    31921 cgggggcttc ggagggcgcg gtgcgcttgc atgatcggtt tctactcaag gcggacttcc
    31981 cttcggcggc cggttgaagg gacccgggtc ccctgcgagg cgccccgtgt tcctgggcca
    32041 gaggataccc ttggcgattc cgcaagtcaa cgccgattca tctgcggtga ggccttcggc
    32101 tgcatccggg aggccgtggc gatgtcggaa gatcgaacat accgccgcaa cccgcaactg
    32161 cgcggtcaag tgaaggttcc cgcatgaaac gcattctttt ccatggatcc cccgggttga
    32221 ttctggcggt cgtgatcctc ttgggaagct gtgaggtgca gaaccggttt ctctatttcc
    32281 ccagcgcgga tcggccgccc gcgaggtcgc tcgctgccga aaacatgagg ctctggcagg
    32341 agacttccgg ggattatcgg ggcttgacgg ccgattgcga ggccgatgcg ccccacggga
    32401 cgatagtcct gtttcacgga aatggcggca cggctctcga ccggggcttc tatttgccgc
    32461 cgctgatgcg acggggcttc cgggtaatcc tggccgaata tccacgctat ggcggacgcc
    32521 ccggccggct gggggaggag gcgttagtcg ccgacggacg ggagacggtg ggatgggcgt
    32581 acgcacaata cggaagaccc gtttatctgc tgggggaatc cctaggtgcg ggggtcgccg
    32641 ctgccgtggc cgggaagtcg ccggttccca ttgccggggt gatcctgatg acgccctggg
    32701 atacgctggc agatgttgcc cggacccagg tcccctttct gccggcaggc ctgtttctca
    32761 ccgatacgta tgacagcatc ggcaatctga ggctgttcaa aggaaagatc gccgtgatcg
    32821 gcgccgagag ggatggcatc atccccgtcg gacacgcccg acgcctgtac gcatccctcc
    32881 ccgaagtcag gaagcgcctg tgggtgatcc ggggggcggg ccacaacgac tggccgcttt
    32941 acgcgggggc gtcgctatgg gaggaggtga ccaacttcgt caaagagtaa acctcgaccc
    33001 gggcgtgaat cgcctgctcg tcccatttct aaaaggtgga ccgtttcaaa ccgatatcaa
    33061 cgaacattac gcttgacttt ctcgtgagtc gggtgcagat gaaaatacag tgaacaaggt
    33121 atagtcaata cgcgaccatc tgattttaaa ccctttttat aaaagccttg ccgtggacag
    33181 attgcagaag gatgacctca actgcgactc attcccggat ggatatggca ggccgccacg
    33241 tggaagaaaa tcatcagcga acgagcacag caggtggccg gggcggaaag aaattaaggc
    33301 gggttatgaa aaagtcatcc gcggtgagtt caacgatcgt ttttttgtgg ttactcatag
    33361 gttgtggcgg ggtaatcatt catcaaacct cacagccaga tcttcagccc atcccctata
    33421 acgacgtaaa aatgagagcc caaaaggtat tctttgaagg tcataacccc tctgttcgtt
    33481 tcaggggcac ggggaaggcc atcaaattca ccgaagacgg atttcggatc ggtacggacc
    33541 cacaggtttt ttgcgagtac gaggtgaccg gaaatcctta tgtgaaagat tgggggccgg
    33601 ccgccttgga ttcgcagttc ggcgttcatc tagacggatg cgacgtgcag acgtggttca
    33661 aacagaaaga atccgccatt ctgtttgcgc aattgctcta cacgttaaag caacaagcgg
    33721 tggaaaataa agttgcacga aatcttccgg ccggtacggc ctccccgatc gaaacgaaaa
    33781 cgatcaaaac cgatattgac gaactgcctg ccatccatag cgaaccgcaa cagaatgcgt
    33841 atgccgttgt catcggaatt gaacattacc gtagcattcc caaatccgat tattctcgca
    33901 atgatgccca tctggtcaaa ggctatctga agaccctggg ttttcaggaa cgtaatattg
    33961 atttgatcac cgacgaaaag gccacaaaga cggatattga aaaatctttg gaggcgtggc
    34021 tcccaaaccg cgtgagaaaa gacagtcgtg tttttgttta tttctccggc catggggcgc
    34081 cggaacccgc cacgggggaa gcctatctcg tcccttacga cggagatccg aattacctgc
    34141 cggtaaccgg ttaccggttg aaaaggcttt atgacagcct gggaaaattg gaagcaaagg
    34201 agatcatcgt tgttctggat tcctgttttt ccggtggcgg cgggaggagc gttttagcca
    34261 agggggcaag gccgctggtc atgatggcgc ccacgggggc cctgccttcc aacatggccg
    34321 tcctgtcggc aacgcagggc agtcagattt ccacgtcttc cccggagaag gggcatggcg
    34381 ttttgaccta ttactttctg aaagcgatca aggatggcaa gaaagacctc gccgagatct
    34441 atgagcacat ccttcctcaa gttgaagacg atgccaagca gctcaatgtt cagcaaagcc
    34501 catcgatccg tccggataca gaaaaaatca aaggcaggtt ttatttggtg aagtgaagct
    34561 gatgagaagg tgcctggtat ttcttttcct tttagttttt ccgtgtgtcg gccttgcggc
    34621 ggacaacccc gtctgggtgg aggccgaagg cgaggcccgg tttggcgatt ttgacaccct
    34681 gaaggaagta aaggatcgcg ccagacgcga cgctgaggca aaggcgatcg agcaggccgt
    34741 gggggtcttc atcagatctc acacgctcat ctccaacagc caggttgccg aagatctgat
    34801 ctatgccgcc gtccggggga agatcaccaa ggaaatcgtc atctcgtcag actgggatca
    34861 tcaagaccgg catttataca gaaccaggat caatgcccgg gtcgaacccg tctatccggg
    34921 aaaaggtgac ggcctgtccg tgaatctgtc tctgtcgaaa accgacctca aggaacatga
    34981 agaggtccgg attttctatc gggcgaataa ggatgcctat atctatattt tttccattgc
    35041 cgccgacggg tccgtgaccc tgctgctccc gaattcgcaa atgccggatc atttctttca
    35101 gggggggaag gcctatgaat tccctccggc ggacagccgc attcgcctga aggctttgtt
    35161 ccttccaggg ttccaaaccg actctgctga agagcgaatc aagatcattg ccacacggaa
    35221 caacgaggaa ctgctcccgc ttgggtttcg tgagggtatt ttccatgcct atgacgcgcg
    35281 atcgacgggg atgatcaacg atctggtgcg ccggctgaac cagtttgagc cgacggactg
    35341 ggcagaggcg acagcgattt acacccttgg aaggtgagga tgaggcccgc cccgaagcag
    35401 cgtggacaga gttcgtttta aggggtcttt ttttaattca gtttatcctc gctcttcgac
    35461 catctcgtta tggagggaag atgccatggc ccccccccgc cccctgttgt tcccgattgt
    35521 actgatggtt ttcttggccg gctgtgtggc caaatcgccc ctgaccgtgg ccatcgaaca
    35581 agggaatgtc gcggtcgtga aaaccctggt cgaccaaggg gcaaacctcc gcgaacttgc
    35641 ggattgcggc tccggcatgt caacgattag aacaacgcct ctggaatgcg ccgtatggtt
    35701 cgaccggaca gagattatcc atctcttgat cgataaaggc gccgatccca gtgagcagga
    35761 ttactacggc ggcagcaccc ccttgtccac ggcggccgcc tacggtatca acagaagtgc
    35821 gaaggtgctt ttggagcgtg gtgcgaatcc gaatgcgggc aaaccgttga gcaacgcggc
    35881 ctatcacggc aatattgagt tgatgaagct attagtcgcc aaaggcgcgg atatcgatac
    35941 ggcgattgag gagctgaaaa cccggtacga ttcctggctg ccggaacgga acaaaaaaat
    36001 cgattcggcc atccggcttc tcgataaatt aaagccaaaa cccgttgtcg ccatacaggc
    36061 tcctgcccct tctgccgcgt ctccgcagaa tattacgaaa gaggacatcg ccacgatcgt
    36121 ccaggaggct gtggaaaaag ccggccgaaa ggaggccagg ccgggcgtcg ccgtatcgtc
    36181 cgagatcgat cggcctcaat ggacccccgc ggaaaggatc atgggggata aggatctggc
    36241 catcgtgatc ggaatcgaag ggtatcagag ccttcccaaa tccgactatt cctatgatga
    36301 tgcgatgctg gtcaaagatt acctgaaggc tctgggtttc aaggagagga acaccgagtt
    36361 ccttaccgat gaacgggcga cgaaatcgag tattgagaaa accgtcgaag tctggctgcg
    36421 caataaaacg cagcgtgaca gcaggatcgt gatctattat tccggtcatg ggtcgccgga
    36481 cccggtcacg ggggaggcct atatcgtgcc ctacgacggc gatccgaact atctttccgt
    36541 gacgggctat ccgctgaagc ggctttatga gaaggttggg gcgctgcctg taaaagagac
    36601 ggtcatcgtt ctcgatgcgt gcttttcagg cgccggaggg agaagcgttc tcgcaaaggg
    36661 aacacggcct cttgtcatat cgaccatgga ggcgcctcgg tatgggaata tggttgtttt
    36721 gacggccact cagggtgcgc agatcagcac ctcttcgccg gaaaagggac atggcatctt
    36781 tacctactac ttcttgaagg cgttgcagga aggcaagaaa agcatcgccg atatctacga
    36841 atacatgaaa ccgcgggtag aggacgatgc gaaaaccctg aatgtccaac agagcccctc
    36901 tgtgagccct gacgtcaaaa ccctgcgggg aagatttatc cttagaaatt agcgggtgtt
    36961 tcatccggaa gaccgaacgg tgcgtcccat caaacaacag ggaggaagag acgatgaaga
    37021 aaacgatggc tgccttattg atgcttgcgt gcgcgatgct gatgctcagt gcctgtgcag
    37081 cccagagacc ctctgcggct gcccagacaa atttggcaca gcttgaacgg caagccgctt
    37141 gagatcgatc attcaagtcc gattcgctcg ggcctgaatg aacgcttttc cggatgcggg
    37201 cagggtgttc gtaagaacga taaggagggg tctgtcgaag cgtgggcacg gccgtccgca
    37261 cccgcaaggg agcggattta tatgcattca cttctcgtgc ggtcggcaca ccacgcttca
    37321 gacatacggc actttgtaac gccgatggcg atcgctttcg ttcccgttct caggaaaccg
    37381 gatcagccgc gcgagaggtg ccacagccag aggcaaaggc cgcccaggac gacgacggcg
    37441 actgcgctcg gccacagggg aaagacatac ccctccacga tgatctccgg ccggacgaaa
    37501 agccgcgtca actgcccgac ggtcatcaat ccgaacagga tgctcgccat ccgcaatccg
    37561 atgattctgg aattcatctc tcgtccctca ctgaggttat gccctcagcg cgtcccgccg
    37621 acaccgcctc tcctggggcc gccggtttta tggctgttcc catagcttgc ttccacctct
    37681 cgaccggtcg gaaccggggt tgctccttcg cccggaatca accgcccggc cattcgcgat
    37741 gatcgacgaa gatcaggttg ccggtgtcaa aacccaatcc ggcggccttc gccacgaggg
    37801 catccttcac gtcctgcttc atttccgggg ctctcgccag aatccataaa taagatttgt
    37861 cagggccgca gacgagtgca tattgataat tctcgcggtc aagttcgaag atgatgtacg
    37921 agccgtaaaa gggaccgaag aaggaaactt tcaggtagcc ctggtccggt cccttcacga
    37981 aataggcctt gccctcggct tctttccagg ccttttcctt cggtgcgtac ccgcggttca
    38041 ggaccctgac gccgccgtca tcccgcaaac tgtaatccgc ggttgtccgg gtcaagcccc
    38101 gttcaaacga atgatccagc ctcgcaattt cgtaccacct tcccagatat ttctccaact
    38161 tgaaatgatc gacgggtttg acctgttcgg ggattccgac acatccggtc aacaacaaga
    38221 gcaatacgat tgctatcttt ttcatctcct ttccccttca acgctcgatt ttcttgtgcc
    38281 tcatccgccg agcacgatgc ttctgagggc ctcttcaatt cccggatacc ggaaccggaa
    38341 gccggccgcg aggaggccgc gcgggatcat gcgcgccttc ttgctttctg cgcgaagggc
    38401 ctcctgtttc cagtcatgcg ccagaccggc gatacgcaca cttgccgcga ttcccgcccc
    38461 gacgcaaggg aagcaccgaa cgcttgggtc gcatcctttc aacaattgta actctctcgc
    38521 cgtaatagat tcaagaagcg agagctttat ccttcccgag gacatggcaa cgattgagat
    38581 tcgcgcgccc atccaagggg gggcagagat attctgttga cagcggaaac ggttcttggc
    38641 agactgctga tccaggaaaa cgggaggatg atatgctcag ctcagcggac caggacaaac
    38701 tcatcgtcat catgaaggcc atggaggaac acgaattggc cgtggctgaa ctctatcaga
    38761 ccgcagcggt tgcctcgccg tccgaccagg ccttctggaa cggtttggcg gccatggaaa
    38821 agacgcatgc cgataacctg gagcggatga tagcccaggt gcaggccaag gcgtcgcgct
    38881 tcgagatggg gaggcccttc aacatggctg ccctgaaaac cgcccaggag ggaatccggc
    38941 aaaatatcag caagattgcc cgaggcgccc tctcgaggct ccagatgctc gtcgtggccc
    39001 gggacatcga gcaatccctc atcgaggccc aatacggctc cgttgtcaag accaacgacg
    39061 tcgagtatca aacgctcctc aaggaaattg tcgaccagac ctacggacac cgaaaagcga
    39121 tccaggagca gatcgacaag gcgcaaaagg gggcttaggc tccgtttcct gaaagcccgt
    39181 cgatgcctac gcctgtgagt ttggccaggc agccggtcag gaaggcgatg tcctcctgtt
    39241 ggtcgatgat gctcccgtgc tggggcgcga ttcgctcggc gcccaccccc ccgatcacct
    39301 tcatcgcccg gtgcagcgcc ttgtccgagg gcatgacctg gcggtggaag gccagcaggt
    39361 cggtcaaggg gcagtagggc tttccatagg cacaaggacc gtcggtgtcg tatgcgtcac
    39421 aggagcggca ctgatcgtcc aacctcagga aaaggtccca acgctgcgag tagctcccga
    39481 aaagatcact gctgaacagg gtccgggtcc ggggatcgta ggtgaccaag cttccggccg
    39541 tgtgggcaaa gggggtctta aagaagcgca gttcccggcc gttgaatgaa aagaccattc
    39601 ccctgtcgtc catggagcgg agaagccccc gtctttcccg ttcaaggtag aaactcagaa
    39661 agacgttgtt cgcccgatcg gagaggatcg taagcgcctc attggcgcag aggtcaacca
    39721 tgttggccat ggaaccgcag aggtccggat cggggtgctg atagatgagg gccacgatct
    39781 gctccggcgc gatcccggtt tggaggatct tgcgcatgac cacagggaaa tcggcgcggc
    39841 tgcccgcgtc gatgagcaca gctttgtccc cttctatgat gaggtaggga ttacagtgga
    39901 aattgctgta gccatcgtga aagcccaccc aatggatccc gggcgcgacc tccacgggcc
    39961 ggtcataacg cgttcgctgt gttttcatgg cgttcccctg ttgcgaaata ggccttcatc
    40021 cgaccccggt gtttgagggg gatctctccc cgctcctcga aaaaaaaccg gttacgggtc
    40081 aaaagatagg tctcgtgcga gcagttcacc cgtccaggtg tcccgccggt ctcgaagcgg
    40141 gaggcgatgt tgacgttgtc gccgaagatg tcgaactgca tacgctcttc ccccaccacg
    40201 gccgctatca ccgtccccgt atggatcccg atccgggttt cgatcttgag atcgcccccg
    40261 gcacggaagg ccttgagctg ttcatgcatg gccagtcccg cctggatggc cagcgaggga
    40321 tgatcggggt cgggacgtgg ggcgccgaag accgccatat aggcgtcgcc gatggtcttg
    40381 accttggtgc cccgatagcg gcgcatcagg tcgtccgcgc ctctgacaat caggtcaagc
    40441 atccggacca gcttttctcc ggagagccga gacgccattt gagtaaagcc gcagatgtcg
    40501 gcgaagagga tcgtgcagtt gcaggtgcgg ggggcaaaac tccccgattc cttgatttcc
    40561 tcggccacct cggcaggcag ggtgtttttc aggagttcgt cgacccgctt ttccagggcc
    40621 acgtattgcc ggatcagctc cttgagaagg cgctgctgga tgccgccatc ctgaatcgtg
    40681 gccacggact ggaggtaatc caccatgacc gttttcctct gcctgctttt ccgctgctcc
    40741 tcccggggcg tgcgcattga agattcatcg ggcactcggc aaccccctcc cgtttttgat
    40801 catgcaattt gctttgaatt ctagactgcg gttgctggaa aagcaaaggg aaaaacaagg
    40861 tgaaaagaga atcatcaaaa agtgccctcc gctgccgcca cctcgcaaga tctcatgacc
    40921 atgtcacggg gaggcctgtt gcacgtcctg cggggaggtc gtcccccgct ggataatttc
    40981 ggagatttgt gattgtaatg cttcacggga gagtgcatcc ggcagcaaaa tacaaaaact
    41041 ttcagaggga aaggacaacc gatcatgaaa aagattgcaa tcctcaccgg aggggcgatt
    41101 ttattgctca tcctcctttt ggcggtgtgg ctgattttcc ggcccgatcg aggaacgacg
    41161 ggggccgtca acacccagtt ccgctggatc ggtccgaacg acaaaatcgt cgtggacggc
    41221 tttgatgatc ccaaggtgcg gggggtggcc tgccatatcg cccgggccca aaccggcggt
    41281 gtcaaaggtg cgctgagcgt ggcggaggac gccagcgacg cgagcatcgc ctgccgccag
    41341 accggaccga tcaaatttct ggatgagttc aaagacggag aacgggtctt cgacgaacag
    41401 cgcagccttc ttttcaagtc gcttcaggtc gtgcgcttct acgaccgcaa gcgcaacgtc
    41461 ctggtctatt tgtcctacag cgaccgggtg ctgacaggca gcccgaaaaa cagcatcagt
    41521 accgtgccca tcatgccctg gccggctgcc ggcgatagcg cgatgatgcg gtgattacat
    41581 ttccccgcta cgtgattgga tcggctgtcc atcggccttg aaacccgatc cgataagggg
    41641 gcagaacctc tgagtttcac ttgactttgg ccgtcgttga gttgtaacag ttgcggcggg
    41701 attcggaatc cggacatgcg gagctcacaa agtaggctga gagcgtgtaa tccacggctc
    41761 cccggttgct atcgccggtg agaatttcga ctcaaattgt ttcgcgcaaa cgtacgttta
    41821 gtggaggcaa tatggatgtc tatggttctt tagtggagat tgtcggagag gagtttgtct
    41881 ccgatcaaga agaggaactc tatatttatt caagagattc cggtgcacaa gaaccccgca
    41941 aggcggatta cgttgtcgcg ccgaagaagg tggaagaagt gcaaaagatc gtcctattgg
    42001 cgaacaggga gaaaatcccg atcactccga tgggcggggg gctcaccttg agcggtttgg
    42061 ccgtgcccat caaaggcggg atcgtcctgg acatgaaaag aatggaccga atcatcgaaa
    42121 taaatgagaa gagccgatat gccgtactcg aagcaggcgt cacgcaaggg gccctccatt
    42181 ctcatcttaa caagcattat ccccatcttg aacattcaat gcccgatgct ccccccatgg
    42241 cgaccattgt cggaaatgtc gtcattcagg ggcatggtca tctcagtgtc tcacgcggga
    42301 tcaattcgca aatggtcaac gggatggagg tcgtgctgcc aaccggcgag gtttgcaaac
    42361 tcggatcctg ttccgtttct ccgcattggt tcacccggga cccgcttccg gacctgatgg
    42421 gtttattcat cggctggctc gggacaaccg gcatcatcac caagatctct ctccagcttc
    42481 ttccaaaacc gaagttccgg gatctgttga ccttcacctc caacgaactc gatctgctgc
    42541 ccgatttcat attcgacatc acgcaactca accttctaga caatctgttc atcatatcgc
    42601 aggaatggcc tccatgggca cagggcatcc tttatgtccc cattgtttcc gcccaggatg
    42661 aggaggaatt tgcactcaag atcaaactcc ataaagagct tttacgcaaa tatgaaggga
    42721 agatcacgct tgtggaagga aaacacgccg aacgaatgcg tgaagccaga ttgcctgtgc
    42781 ctccggttac cctgggcgtc atggcggcgg atacgaaaca ggggggcggt ttcgaatata
    42841 cgggagggat cgtgccgata gagaaggttc ccgaagcatc gagcagatcg gtggagatcg
    42901 cccacaagta tggacagcct taccttcttg gatatcaact cctcacaggc ggacacagca
    42961 tgatgtgcgg tctcgcatat tgtctgaatc gcgccgacga tgaggatgtc gagaaagtca
    43021 ggaaggcaat cgatgaaacg aacagaatga tcttggacgt ggggggggtt ctatggaagg
    43081 ctgaagcacc ggcgcagcgg ttgatgatgc agaggatgga tcccaacaca attgaactca
    43141 tgaaaagggt gctgagggcg ctggatccga acgcgatcat gaaccctgga aattggagtg
    43201 aataaatggc acttgcagat tacgaaggca tgatccacag atgcttcaga tgcggctatt
    43261 gcaaatttac cagcgattat tcttacgtgg ggtttaattg ccccatgtat aacaaggtcc
    43321 gtttagaaag ttattccccc ggggggctga tgtggctgat ccatgccgcc ctggtgaagg
    43381 aaaaactcaa gcccagcaaa cacctgtccg agatcctgta ttcctgcacc ctgtgccgga
    43441 attgcagcga ggaatgcaga tttcagtttc atgaggacat cgtccacgtt ctgaaagcgg
    43501 cacgggaggc catggtggag aaacagctcg tgcccccgat ggtcagccag ttcttgaaaa
    43561 atgtttacga ctacggcaac ccgtatcggg agctgaagga gaacaggggc cattgggctg
    43621 aaaatacagg aatccggcaa tatgaaaaag gcgatgaatt tctgtattac gtcggttgcg
    43681 tcggatcata cgacaccgtc agccagaaag ccgcccgggc gttgggccgt gtgctgttga
    43741 aatccggtct ttccttcgga atcttgggga gcagggagat ctgcgacgga aatgaagtgg
    43801 acatgctcgg cgaaaaaggc ttgttcgaat tcctgaagga ggaaaatacc cggacattca
    43861 aggaactcgg agtcaaaaag atcgtcaccc tctcgcccca ttcatatcat gccttgaaaa
    43921 atagctactc cgatgaattt gaggtattcc attacacgca actcttccgg gatctgatca
    43981 aaagcggcgc gttggatgtt tccggaggat tcaggacgaa agtcacgtac catgacccct
    44041 gttttctagg caggtacaat cacgaatacg acgcgccgag ggagattctg accgccattc
    44101 ccgggatcga gctgatcgaa atggagagag cgaaggaaaa cgctttttgc tgcggtggcg
    44161 gtggcggcaa cttctacacg gatatcctcg ggggtgggga gagcaccccg agcaggatca
    44221 gggtcatgga agctcgcgag acgggagcaa agattctggc ggtgtcgtgc cctgtctgta
    44281 tgatgatgct ggaggacgcc ctcaaggcag aggggttgga aaacgagctg gccatcaagg
    44341 atatcgccga gatcatggac gcagtatgcg gtgattcggg cgcttagggc aggggcctta
    44401 actcaagaag gcttctcgtc ccggtagaag cgttcgacat ccttcaaata gtccgccagg
    44461 tgtttgcggc tgacaacaaa gatatgcccg atattcttct cgatggtccg gttgaccggc
    44521 cccttgggaa gccggatctt accgtgattc acctgccccc aggtctcgct ggccacgcat
    44581 gatccggccg gcgcgatggg atcccgcgat ccccgataat cgaaaatggc cacgcccgcc
    44641 ttcttcagga tcttcatatt caccggtttg ccgtcgaatt tggggttagc ggaaggaagg
    44701 gcgtatttcc cttcgaaaat ctcgtttccg agaaagatct tctggatcca ctcaatgtgg
    44761 cagtgaatgg ggaatttggt tccgcgcgtc agccagaaga gaaagggcgc ggcgtcaagg
    44821 atggcttccc tgctcacggc acgaccgtaa aaacccagga cgaccgtgta ttgaacgccg
    44881 ggctgaattt cattcatccc cgcttcaatg atttgcggcg ggatattgac cgcaccaaaa
    44941 agctctttca tcagataagg atcgtaatcg cggcggatca gcgttcgaat cgggccatgc
    45001 ccgctgtctc catcgtcaaa cttgaccggc gaagccatta acgcgatttt ccggatatcc
    45061 atcgggaggc ctgccgccat ccgctcctcg gcccgccggg cgagataagg caggatgagc
    45121 gtgccgccca tgcagtaccc catgatatag atctcgtttt ggggatgacg ttttttaatc
    45181 aggtcgatgt agagatcggg gatggttttc ccgtaaaagt ccaaccccag ttcggtttct
    45241 tcccaacccg gattaccgtg gtcgaccagg tagatcatgt agcctttttt gaggagcgct
    45301 tcgacaacgg atttgtcgca agccagatcg aacagctccg gccggttaat gagcggggtg
    45361 accatgtaga tgatcttccc gttgggttta atcccttgag ggatcggata atagcgtaac
    45421 cggacccgat gcaattcgga tccctccaca aattcatacg cctggcagcc gagttcgccg
    45481 ccggtttttc tctcggagaa ttctttgata tcgaatgcat tttccgcatg gagattttcc
    45541 agccgccgat agttttcc
//
LOCUS       DAOOLJ010000015        41725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_686_length_41725_cov_18.809719, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000015 DAOOLJ010000000
VERSION     DAOOLJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 41725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41725
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_686_length_41725_cov_18.809719"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLW16_04540"
     CDS             complement(<1..143)
                     /locus_tag="PLW16_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70207.1"
                     /translation="MTETDPQRIYIFDTTLRDGEQSPGASMKPSEKLLIARQLERLGV
                     DII"
     gene            complement(617..2275)
                     /gene="ilvD"
                     /locus_tag="PLW16_04545"
     CDS             complement(617..2275)
                     /gene="ilvD"
                     /locus_tag="PLW16_04545"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPB70208.1"
                     /translation="MRSDLMKKGPERAPHRALLKAMGYTDPEIRRPLIGVVSAASDVV
                     PGHVHLDLLAADVKAGIRSAGGTPMAFPVIGVCDGLAMGHTGMKYSLASRELIADSIE
                     VMAMAHPFDGLVFLPNCDKIVPGMLMAALRLDLPCLFVSGGPMLAGHAGGQPVDLISV
                     FEGVGALRSGRIKAGDLKALEDAACPGCGSCAGMFTANSMNCLTEAIGLALPGNGTIP
                     AVHAARRRLAKTAGVKMMALVRRGLRPRRIARMEAFRNAMTIDMALGCSTNTVLHLPA
                     IAHEAGLTLDLDLFNAVSAQTPNLCRLSPAGPHHLEDLDRAGGVPGVMKTLAPSGLLD
                     LKAMTVTGMTLGETLRRARVADREVIRPLERPFSATGGIAILKGNLAPEGAVVKASGV
                     APEMLVNEGRARVFDGEEAAMAAILAGAIRPGDIVVIRYEGPRGGPGMREMLGPTAAI
                     AGMGLDKTVALLTDGRFSGGTRGAAIGHIAPEAAAGGPIALIEEGDRIRIDIPGRTLS
                     LQVPAEMLAERRQGFRPRPPAPLTGYLARYARQVASAGQGAIVL"
     gene            complement(2363..3427)
                     /gene="ilvC"
                     /locus_tag="PLW16_04550"
     CDS             complement(2363..3427)
                     /gene="ilvC"
                     /locus_tag="PLW16_04550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPB70209.1"
                     /translation="MARINFGGTWEEVTTAEEFTLERARRALAGETVAVVGYGVQGPG
                     QALNLRDNGVRVIVGQREGTGSWDKAVADGFVPGETLFPIAEAARRGTVVQYLLSDAG
                     QKALWPTLAPHLTPGKALYFSHGFSIAYRDRTGVVPAADIDVILVAPKGSGRTVRTNF
                     LQGSGINASYAVFQDATGRAEERTLALGIAIGSGYLFPTTFEKEVYSDLVGERGVLMG
                     CLAGVMEAQYDLLRRHGHSPSEAFNETVEELTQSLIRLVAENGMDWMYGNCSTTAQRG
                     ALDWKGRFREAVAPVFDALYASVVSGEETAIVLDVNGAPDYRERLEKELAAIRASEMW
                     RAGAAVRALRPEKRLRPAGK"
     gene            complement(3622..4125)
                     /gene="ilvN"
                     /locus_tag="PLW16_04555"
     CDS             complement(3622..4125)
                     /gene="ilvN"
                     /locus_tag="PLW16_04555"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPB70210.1"
                     /translation="MEPPPEIHEEQILSILVRNRPGVLSRVAGILGRLGYNIASLCVA
                     ETTDPAVSRITLTSRAERDFTDKIRKHLDKLVDCIEVADYTGTPFLRRELMLLHVTFR
                     EEERAEILRAIELCQGRILLLTEGICLIEAAGDPEHLGGILACFRPFGIRAVNRTGTI
                     ALPRRPS"
     gene            complement(4211..5884)
                     /gene="ilvB"
                     /locus_tag="PLW16_04560"
     CDS             complement(4211..5884)
                     /gene="ilvB"
                     /locus_tag="PLW16_04560"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPB70211.1"
                     /translation="MSPLIGADILLDSLRQEKVEAIFGYPGANTLPVHDRIQATAIRH
                     YLMRHEQAAAHAADGYARASGKVGVCLATSGPGATNLVTGIATAFMDSIPLVALTAQV
                     NTNLLGRDAFQETDIIGITLPITKHSFQVLDVRHMADTLREAFDLARGGRPGPVLVDL
                     PRDILAAPAGGDAAPSRPETRPAPTAPDAAPLEELARLLNRSERPVILIGGGAKLSGA
                     CGELEALVAKARIPFATTMMGLGAVASGNGYNLGFIGTHGHELANRIIHQADFILAVG
                     ARFSDRSTSKTEEFAPLATIAQIDIDPTSIGKNLKADCPILGDIRDVLRGLLPRVERR
                     EASGWMERIEAERAVLREGRKGSGDGPAGAFIRQVQCALPPQAIAVADVGLNQIWTLR
                     NWQPRTPRSILTSGGMGTMGFSIPAAVGAQIARPDHAVVAITGDGGCYMNIQELATIG
                     YYRLPVKIIVINNGHLGMIRQLQDLLFDGRLNTIALGGHVDLTAVARGFGIPSARTDM
                     EGDTAEGLDRLRRSEGPFLLEAVIDPERYVYPLIPPGRSNMEMIYGRTA"
     gene            complement(6212..8239)
                     /locus_tag="PLW16_04565"
     CDS             complement(6212..8239)
                     /locus_tag="PLW16_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPB70212.1"
                     /translation="MTGGRGSKTSRGTADGQRGYASLLIILGALGVVLLGTSLVFYLL
                     LMELPSIAALKDFRPSIATRVYDDNNELIDEFFLEDRKVIKLSECPRYVIQAFVAAED
                     TRFFEHRGLDYQSITRAFFKNLAAGHIVQGGSTITQQVAKALYLSPERSYRRKIKEAL
                     LAYKIDRYLTKEEILNLYLNYIYLGHGTYGIEAASQGYFGKSARHLTLSEVALLAGLP
                     KAPNNFSPFLHPDRSLQRQAYVLQRMLDDGYITAAEKERALATPLRLRSVRPKDKVAA
                     YFVEHVRRYIMEKYGSDVLYKEGLEVYTTLNLPMQKAARDAVERGLQEMERRQGYAKG
                     LVQGALLCMDARTGAVRAMVGGRDFSRSEFNRATQSRRQPGSAFKPFIYTAAFDKGLT
                     PSTIMVDSPISFAAPSEPNGVWQPKNFDGTFLGPMTLRHALVHSRNVVTIKVLKEIGV
                     DYAAGYAANMGIASPLARNLTLALGSSGVTLLEMVRAYGVLANQGQRATPLFIKKIVD
                     RTGQVFEENQPMLEQVIDPRIAFMSAYVMQDVVEQGTGARVRSIGRPVAAKTGTTNDM
                     RDAWFMGFTPSFVTGVWVGFDQERSLGRQEVGGRAAAPIWLYFMEKAVQGTPVEVFPV
                     PEGISFVKVDPNTGASLPDGTQGGLYESFLEGTTPEEVPTYGPADEQEGFF"
     gene            complement(8236..9009)
                     /gene="tatC"
                     /locus_tag="PLW16_04570"
     CDS             complement(8236..9009)
                     /gene="tatC"
                     /locus_tag="PLW16_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPB70213.1"
                     /translation="MEKPAEDKMPLTSHLDELRTRLVRILIALGVGFGVCYVFKDWLF
                     VVITRPLVAVLPKNSHMVFTGLPEAFFIYMKIAFFASLVLTSPYTLYQVWKFVAPGLY
                     REERRYGLAFILSSTLLFTGGVLFGYFIALPPAFAFFVSFTTEMLRPMISFREYLSLA
                     LKFLLAFGLAFELPVFIYFLTKVGVVNVRILRKQRRYAILMIFIASAILTPSPDAVSQ
                     IIMALPLMVLYELGILVAKVAERKKAADSGIENGKESPS"
     gene            complement(9066..9380)
                     /gene="tatA"
                     /locus_tag="PLW16_04575"
     CDS             complement(9066..9380)
                     /gene="tatA"
                     /locus_tag="PLW16_04575"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPB70214.1"
                     /translation="MFGIGTPELIVILIVALLVIGPKRLPDLAKSIGKGLQEFRRATD
                     GVTDTLKDTLREDEEKPEEQEKGAGLKDSLLYGKSDTDSEPADEKAGTVPPDDTPAAP
                     TT"
     gene            9551..10606
                     /locus_tag="PLW16_04580"
     CDS             9551..10606
                     /locus_tag="PLW16_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPB70215.1"
                     /translation="MFRKTGVVKDRRYLDHGRDIAHPESYKRLQALYEILEAPNMMGK
                     FTEVEPRHATYAELEMIHERSYIDFVADTAGKEMCSLDPDTITTAETYDVARLAVGGL
                     CRAVDAVMAGEVENAFALIRPPGHHAEADEAAGFCIFNNVAIAAKYALEKHRLKRVLI
                     VDWDLHHGNGTQNAFYADRRVLYCSTHESPGYPGTGALEEIGQGKGLGYTVNIPLKSG
                     AGTAHYVKVFRRLIVPVAMTFRPDLILVSAGFDIFSGDPLGGMKVTPRGFSYLTRILM
                     DVADACSNGRIVLSLEGGYSVEGLTMSVTAVIKELSEATHATEDELRDMEASADTRQD
                     AVIRAVMNQLNPFWKVY"
     gene            10757..11044
                     /gene="gatC"
                     /locus_tag="PLW16_04585"
     CDS             10757..11044
                     /gene="gatC"
                     /locus_tag="PLW16_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPB70216.1"
                     /translation="MKITRAEVEHVAHLARLEFDEAETEKFTSQLNDILLYMDKLNQV
                     ETRDVAPMTHAIARTNAFREDRVRKSLPLEQALANAPDPRGEFFRVPKVIE"
     gene            11146..12603
                     /gene="gatA"
                     /locus_tag="PLW16_04590"
     CDS             11146..12603
                     /gene="gatA"
                     /locus_tag="PLW16_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPB70217.1"
                     /translation="MELYTLTIHELQDRLRAGETTAEALTRAVFKRIRAVESDVHAYI
                     TLLEEPALAQADRADRAIRDGDLRALTGIPIALKDVFCTRGFRTTCGSRILENFVPPY
                     DATVVEKLAAAGAVFTGKTNMDEFAMGSSTETSYFGLTRNPWDLDRIPGGSSGGSAAA
                     VAADECIAALGSDTGGSIRQPAALCGVVGLKPTYGRVSRYGLVAFASSLDQIGPFTKD
                     VEDAAILMNVIAGYDPRESTSVPEDVPDYRAFLGRGIDGWTVGIPKEYFVAGLDPEVE
                     RAVRDAIGVIEGLGARCVEVSLPHTEHCLAVYYIVAPAEASSNLARYDGVRYGYRSPD
                     GRDLMEMFKKSRSAGFGAEVKRRIMLGTYALSSGYYDAYYKKASQVRALIKNDFDTAF
                     RTCDALVTPTTPTPAFRIGEKTDDPLQMYLSDILTISTNLAGIPGISVPCGYTAAGLP
                     VGVQFQTAHFAEGKLFRIAAAFEKHANFTKRRPKL"
     gene            12603..14039
                     /gene="gatB"
                     /locus_tag="PLW16_04595"
     CDS             12603..14039
                     /gene="gatB"
                     /locus_tag="PLW16_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB70218.1"
                     /translation="MEYEPVIGLEVHAQLLTDTKIFCGCTTQFGAAPNTHTCPVCLGM
                     PGVLPVLNRKVVAFAMKMALATDCRINPACRYARKNYFYPDLPKGYQISQFAEPLAEF
                     GHVDIESHGGKKRIGITRIHMEEDAGKLIHDEYNPWSYVDLNRTGVPLIEIVSEPDMR
                     SAEEAAAYLKRLHETLVYLEICDGNMEEGSFRCDANISLRPKGTEIFGTRTELKNMNS
                     FRNVQRALDYEIKRQQYVLEGGGEVVQETRLWDDTAGVTHSMRGKEEAHDYRYFPDPD
                     LVQVEVDETWIAQIRNDLPELPQEKRERFLKDYGIPAYDAGVLTSSRALADYYEAVVR
                     LSGEPKTASNWVMGEVLRFLNEEKRDIRNCPIRPEALAAMIALIKDGTISGKIAKDVV
                     EEMYRTGKFPEDIVREKGMVQITDEGALIPIIDKILAKHPGQLEQYRAGKDKLFGFFV
                     GQVMKATRGKASPQLINDLLQRRLAGDR"
     gene            14064..15218
                     /gene="mtnA"
                     /locus_tag="PLW16_04600"
     CDS             14064..15218
                     /gene="mtnA"
                     /locus_tag="PLW16_04600"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPB70219.1"
                     /translation="MTPFIPASPAPSRWTDMEDAIHTLFWRDDTVVLLDQKALPREER
                     YVTCTDYREVVAAIRDLTVRGAPAIGVAAALGIALGVRHLDAASIDAFREAFWNICGD
                     FAAARPTARNLFWAIERMQRRFDAALVDAAGSAVPPGSGSLPRGRGERDRLRAVSDAL
                     IAEAIRIGEEDLAINRRLGRHGSVLIKDGARILTHCNAGALATAGYGTALGVIRAARE
                     AGKALHVYVDETRPVLQGARLTAWELTKEGVPCTLITDNMAGFLMQRGKIDLVIVGAD
                     RIAANGDTANKIGTYALAVLAHAHGLPFYVAAPWSTVDFSLADGTGIPIEERDPGEVV
                     RFAGVATAPEGIAVYNPAFDVTPHRYITAIITEDGVLTPPLRRPHTRDGR"
     gene            15297..16307
                     /locus_tag="PLW16_04605"
     CDS             15297..16307
                     /locus_tag="PLW16_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70220.1"
                     /translation="MAQAFQTRDADTGGDAGDFVRTFLFGSAGVRREGRTLRSVIVMD
                     EKKQGWVGIPHGGIAMGAVADLAGDLTEECPGPFGPYPYAIDYRMGGASARLGDTLAI
                     EVTATASGARGSIVKDPRTPPYLSASVRFGGDAPSTERRVPAGLPARIDAWAGRLLDL
                     PFYRNCFVCGIARRQTGLGRRFYVPDVPDSPATVVSPIGCLGDDGAAFWRFQRQGRLH
                     PLPLVALLDEIIGWGGFLLTASGGVTVRASYTFYRDIAAGEPLLVFGRGEKVRGNARS
                     RLLFWASGGAAAVRSDGGLEPVAAASGQYLGIGALTEQMKRELLPADLTRRAFQLAGA
                     DV"
     gene            16378..16992
                     /locus_tag="PLW16_04610"
     CDS             16378..16992
                     /locus_tag="PLW16_04610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB70221.1"
                     /translation="MKKLFLFDFDGVLVDSLDFYVQAVGACLREIGTPIVQTREDYLA
                     LFEGNFYEAMAERGVDLAAFARAAHEILPRFNYDALSPFPGIAPVLETLHRDHLLAVI
                     SSNGSPTIRAMLKRFQYDGYFRDIFGSDFLFSKVDKINHALEAFGMAADRTFYIGDTV
                     GDVLEAREAGVKTVAVAWGWHGREKLADAGPDHLFDRPEELLGL"
     gene            17018..17236
                     /locus_tag="PLW16_04615"
     CDS             17018..17236
                     /locus_tag="PLW16_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPB70222.1"
                     /translation="MKRLYLATDDRKIGGVCGGIAEYVDRDPTLIRVLFLLLVIFSFG
                     FGILAYLGMWLVIPKKPASTGGGARGDG"
     gene            17276..17839
                     /locus_tag="PLW16_04620"
     CDS             17276..17839
                     /locus_tag="PLW16_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB70223.1"
                     /translation="MDYETLLKRRRAIRDFQDREVPLPVLEEILRETCLAPTASNRQP
                     CKFIVVRDRERMRRLSDVSRRTLLADLDRNPDSPLKAYEAYLRDDGVNVFYNAPCLVF
                     ILGPDDVRSLDVDCGLTAAYFMFAATARGLGTCWIGLGAHLKDPKVLAELGVPEGCRI
                     VAPLILGYPTAVPPASERHAPEIVKVI"
     gene            17868..18758
                     /locus_tag="PLW16_04625"
     CDS             17868..18758
                     /locus_tag="PLW16_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70224.1"
                     /translation="MTGNPNDEDLRERIRALEEESAQRRRVEEALRESERRYRFLAER
                     INDILWIMDLDLRTVYVTPSVQTVLGFTPEERLRQNVEEQLAPDSLSTALETLSREIA
                     FEKQHGADPDRSVTLTLEFYHRDGSTRWLETIISGIRDDRGVLTRLYGVSRDVTRRRA
                     AEEALKTANKQLEEANRSLGLAYAWMRDRKDQLKQQLFREEMGILLNNEGLIEGLSEK
                     VMEHLGRSRDRLIGHPVRDLIKEEYREDFSREFRQAWMGPSQEVFVEFLDTKDRSKIF
                     DVKLTRLTLSGSRLVLMTLR"
     gene            18817..20748
                     /locus_tag="PLW16_04630"
     CDS             18817..20748
                     /locus_tag="PLW16_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPB70225.1"
                     /translation="MKKTVMAIFTSLMMLMAVPAVAENFTVQGDMASTIHYELQHQIV
                     AGDSMRKLMLSFVVPESFDSPTYKQKITRFKVRFSPEAEDETTTTNARGNKIIQATWY
                     TVPRVVDATIAFDAQTTTGLNPIQSTAPFPAVSTPEDLKDYLQASELVQTGDPAIRAL
                     ALELTQGVKTQFDAVQKVVSWVVDHVRYVNPPVQYDALYSLRTGKGNCQNYSHLTAAL
                     LRGVGVPVRIVNGVTMNQPFDVSWERGTLTFKMGQGRHSWVEVWFPDLGWVPYDPQNM
                     QFFISNRFVRIEVGVDNNETKNDGLVRWAQSSGARTKPTLQETIGGNFLADSIQVSAK
                     RQNYGPKNLLLGPNVLARLEEAAVQPAPEPAPAPPIPVPPVPAPEPMPPAPAPEPVPK
                     PAPAPPVPVPPVPEPVPAPPEPAPEPAPAPPIPVPPAPEPAPAPPIPEPPVPAPVSPK
                     PLRYDKPFVFGNLEFPEDVDFAFPRVTQAKGGGSFEMSRNFLVETAEFVTHGVTQYAQ
                     VVELAKPATLHEIALALHNFGGDGHLWVDVFKDQDGQPGEILCTTQMVALEEISGRPG
                     YRWVTFTFPDRERPVLAAGNYWIALGFSGKPVVNWFYTYGKPVGPVYGTRYKSIFAKE
                     WSGALNYEFNYKVVGMATE"
     gene            complement(20840..21331)
                     /locus_tag="PLW16_04635"
     CDS             complement(20840..21331)
                     /locus_tag="PLW16_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB70226.1"
                     /translation="MPIYEFYCAKCNTIYNFFSRSVNTDKIPTCPRCKRVRLKRQMSV
                     FAKLSGGKDEPPADDMPPIDEAKMEKAMAMLAGEADRINEDDPRQAAALMRRLSDATG
                     LKLGAGMEEALSRMERGEDPEKIEAEMGDLLEGEEPFLLEAKGRKGGRKSRPAVDETL
                     YDL"
     gene            complement(21479..23725)
                     /gene="feoB"
                     /locus_tag="PLW16_04640"
     CDS             complement(21479..23725)
                     /gene="feoB"
                     /locus_tag="PLW16_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPB70227.1"
                     /translation="MQDERIAITAFREGEEGVIHAVSGGRGLTSRLAGMGVAQGARVR
                     VTHNSGGWVILQVSDTRVALGRGEAAKILASNLREAPEAPAPRRASRGLTVALAGQPN
                     VGKSTLFNILTGLSQHVGNWPGKTVEKKEGTHATEAFEMRIVDLPGTYSLTASSEEER
                     VARDFIIDVHPDVIILIVSAAALERGLYLLSEILLLESPVIVAVNMLDVAEAQGIEVD
                     PQALERALGVPVVPMVATKNRGIRELVARLTALAEGKEPGHPRIPAIHPDHEEIYRKI
                     AAAIDPYVEHPYTVRWTATKLMEGDPEVTKIMEGVIPEAVWNPIQALLVRHEDSLHAV
                     VAGRYEWIESVTRAAVSRHRLGEVLTTDRIDHVLTRPLFGIPILFAILALVFAFTYRV
                     GFPLQAWLERLIGSAAGVLRPALAPLPDWLGGVLIDGVVGGVGSVLTFLPILIIFFAV
                     MAFLEDVGYMARASFVMDRFMHLIGLHGKSFLPLCLGFGCNVPSVMGARIVEAKKERL
                     LTIFLAPFIPCTARLAVITLVTGAMFGSQAISWATGLLALNLLLLGVTGMVISRFFLK
                     GEATPFIMELPLYHRPDIRTIGTVVWMRTAAFVRKAGTIILAVSVAVWLFSYLPYGQV
                     ETSLLAGFGRLLEPLGRPLGLDWKMVTALLTSIVAKENAVATLGVLYAVGDQGLVAML
                     PRIISHASAAAFLVVLMLFIPCAATVAVMRRELADNRWFAASLAFMFALSYAGGVIAY
                     RLVLLTGV"
     gene            23982..24407
                     /locus_tag="PLW16_04645"
     CDS             23982..24407
                     /locus_tag="PLW16_04645"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPB70228.1"
                     /translation="MTRHLHVTCAIIERDGRVLAAQRGGGGSMAFKWEFPGGKIRPGE
                     SPEACLHRELMEEMGLRIDVKRPLDPAPHRYPLFDVTLYPFVCTIAAGEPVLNAHRAV
                     VWLAAEELPALDWAEADRPVIAAYRESLRGGDPSPPIPK"
     gene            24493..26886
                     /locus_tag="PLW16_04650"
     CDS             24493..26886
                     /locus_tag="PLW16_04650"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPB70229.1"
                     /translation="MKKGLGIVSVLIVVALGAYLCFQVFFRMAVPGDTGTLEIDGLRS
                     AVEVRFDDYGVPHLFAKNDDDLFFAQGYVAARERLFQMEISRLAGRGELSSLFGEKTL
                     DKDRFLKTIGIHRLARTGYAALSPEARRLTAAYVAGVNACIRDTKRLPREFILLGAKP
                     GRWEPEDCVATVLLMSYSLTRSVYVDLVLYRIGERSGEGLVKAIAPSYPDFAPTLTGR
                     RLSPVPRGLLKGVFHRFAAADPGDIAPGRPVQEMPASNWMIFSGRMTETGKALFAGSP
                     DLKPTLPALFYIMHLKGGSFDVAGGAIPGAPGIGPLGYNGHIVWSAVNGRGDELDYYV
                     EKINPQNPDQYLTETGYRTFMTREETLRIKTKGGLKQETFLVKFSRHGPIVSGVMPMA
                     PAHCAMKWAAFENPATDLEGLLHLNRAKNFTAFRRALKFVRNTNLGVGYADREGNIGW
                     QFTASAPVRKNGDGSYPVPGWTGAYEWTGYLPYERLPYDYNPAAGYVASFNNDPGNAS
                     DHLTNYYLFERATRFGNIMKERGGRKVGIEDLRAMQLDTVSVVAQRWVPKIRSACADE
                     AYARYTALFEGWNGAIDIGSPAATLFNAFYARFLKNTLGDEVGPKLWEEGLSQSYLFY
                     IPDLVLARIVDEPGHILYDDTATRDRRETRDDIIRKSLQEAVGQLTDRLGDDPRKWQW
                     GRVHKMYFDHPLGAKLGFFNLKPIPTHGEHHTINSGFWDLNQPFRMDAGGVIRIIVDF
                     ARPENSTIISPPGQSGVYKSRHYDDLAQLWADGGQIPLHFFTARDLPNVLRLQPKGR"
     gene            27357..29147
                     /locus_tag="PLW16_04655"
     CDS             27357..29147
                     /locus_tag="PLW16_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70230.1"
                     /translation="MRDIVNPIEIRLTQGGGTPVGLKKVASPVMKTDACINCGTCLRA
                     CPTGAIHELQRQICRLCPDCADSPILFPRDMEALTAESCAGACPIGHYPEGYVNQVAE
                     GAFEKAWERIIAVNPLPSVLGRICSRPCEDACKRGKLIDAPLPIRAMKRLVADMAYEK
                     GWVEKGCYPRKYGERVAIVGAGPAGVAAAHDLSMRGYECVIHDASPSFGGMLAKSVPS
                     FRLPAQALERDFKAILARGVTFRPNIEIGRNPSIADLLARECDAVLVAAGAPKGVKLP
                     IPGADFMGVFNAVDFMTSVKAGFPLKVGEKALVIGGGSVATDVARTLLRLGAFDVAVA
                     CVEGECDMPAFSWELEEAEDEGVRFIRSAAPVAIGGDWQKVQWVDMDPVTRFALGECG
                     IECDTDSTGRFRVEADTVVFAVGQKPDPGVFAKTAGVEMDARGRVRIDPSTQMTTLAG
                     VFVAGDLVEARGSVVEAVASARRAATAIDAYLRGRTAPAPKEKAPVGAPIDEKIFPVR
                     LEKLNVAGIPALLPEDAVTGFDEVEGAMDVAQAVEDARRCMKCGYVDVRHDLCIGCGT
                     CAAVCPQGDVIRMDRPRTPENAAPAKEVQS"
     gene            29144..30271
                     /locus_tag="PLW16_04660"
     CDS             29144..30271
                     /locus_tag="PLW16_04660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70231.1"
                     /translation="MKTWDVIVVGAGPGGSTTAARLAMAGCDVLLVDKERFPREKPCS
                     DIYGRYGVDAYKEIGAYDELLEKGFLFPELMLTSPDYTALKGPNVLSVTCPRRVGDNI
                     LKEAAARQGAKVLEAFWVNDLMMDRGRVVGVKAKYEGKFVEYPARIVIGADGSHSWVA
                     KHLGLFSDNYDEVFLAGRAYYADCDPPLRTMEVHYDPYFFPGFVCLSPHPGYGDVVNV
                     GIGYQMTPYVQALEDVEALTRKFIETSPFGEKLRKARKVSDWMGWRVPSAGQIGKTYA
                     AGAMLIGDAGSFVDPFILEGIANSVRSGGFAVQTALEALEKGDFSESFLSVYEARWRE
                     KMKPRLDALQQMALVARNAEMLNATFQSLKANPAALKKMFG"
     gene            30383..31816
                     /locus_tag="PLW16_04665"
     CDS             30383..31816
                     /locus_tag="PLW16_04665"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPB70232.1"
                     /translation="MGIMTSEAYLASLAKQKPVVYIGGEKIHDVVHSPYFRISLEEMC
                     KFYDWANDERTAADFTFRSHLIDDRVSFWTHLRETPEDVRRMIEVMKAQNARHFCSMC
                     MITGLNVLWAVTYDIDRAKGTQYHERLKAFFRELQTNDFRYCMGVMDPKGDRSLAPSA
                     QADPDLYLRVVERKTDGIVVNGAKLHTSNAPVTHFIFTCPSGVLAEADRDYAVSFAVP
                     VDTPGITYITRPAPGPPAPRTLESPVSGHIGFVECLTVFDHVFVPWERVFMCGEWEFT
                     PNLIRYFSPYVRTCKGTCTSARTDILIGAAALISQYNGTARASHIRSKLTDMMIASEI
                     GWGCALAAVAKSVMHPSGIPIPDTSVANAGLYHGRLRFVEFLGALQEIAGGIVTTMPA
                     ETECLNEASRAYIDKYLKGVDDVPTEDRLKLLYFIQDLTASRFGGYLTASAVCAGGTP
                     ETNRIEVFRNYDLPQKIRNVKELCRLA"
     gene            complement(31860..32591)
                     /locus_tag="PLW16_04670"
     CDS             complement(31860..32591)
                     /locus_tag="PLW16_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB70233.1"
                     /translation="MMDRVIGILFLVFIAVTSLFFFAIALAIWLLTVLFDRRLLLLHY
                     YSSFWASFYLWVMPAWSVSIEGRERIGRKTYVAVSNHQSLLDILVVFRIFFPFKWVSK
                     AEVFKIPLIGWNMRLNRYILLVRGDKDSIRQMLEDCNRALAAGNSIFMFPEGTRSETG
                     LLRPFKTGAFSLARDNRLPILPVAISGTRDALPKYSFKFHGVKKIRVRILEEIPCEAF
                     AAMSVEETAAMVRERIAAHVDPPTP"
     gene            complement(32970..34208)
                     /locus_tag="PLW16_04675"
     CDS             complement(32970..34208)
                     /locus_tag="PLW16_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPB70234.1"
                     /translation="MAATALRSILVKPAGPDCGMACGYCFYSGKAGLYPGTPTHRMSD
                     AVLDETLRQFMASPAPELSVGWQGGEPTLMGLPFYERAVRLEEKYGGGKTIGNGLQTN
                     GLLLDRRWARFFRKYAFLIGLSIDGPQSLHDRYRTLRGGGGTWSRVRDAARMLLDAGV
                     PVNALTVINDVTVRYPEEIYDHHRALGLDYMQFIPCVETDPSDPGRAAPFSVNAEAYG
                     VFLCRIFDRWRADFRDGRPQTSVRFFESLLFGYAGMAPPDCTLCEHCGDYLVIEHNGD
                     VYPCDFFVEPAERLGNLMREPLAGLFVSDRQREFGRRKASLPGACTACPWLTRCRGGC
                     PKDRRRDPRDLGVSHFCGAYKMFFAHADREFGRLVDDWRRRTASEDARRRQEAAGGRT
                     GRNAPCPCGSGLKFKRCCGR"
     gene            complement(34403..35632)
                     /locus_tag="PLW16_04680"
     CDS             complement(34403..35632)
                     /locus_tag="PLW16_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB70235.1"
                     /translation="MINIPATFRISLRALRANKMRSGLTMLGIIIGVAAVIIMLAVGS
                     GASRRISQQIASMGSNLIIVVPGSSTAGGLRMGAGTRSTLTLGDAEAIARECTAVADV
                     APMHNGTAQVVYGNQNWATGIEGSSPGILNVRDWTLAAGRPFTDQDVRSATKVCLLGR
                     TVADNLFGSADPLGQIIRIKKIPFVVIGVLAPKGQSMMGQDQDDTVIVPITTAQRKIF
                     GTRLPGMVRHIVVKARSADEMATAEAQITELLRERHRLTPREENDFTVRNLTQIMQAA
                     EQSTRIMALLLAAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRLQF
                     IIEALTLSLIGGVIGIVLGVTGAKLLSILAGWSTVISVPSVLLAFGFSGLVGLFFGFY
                     PAYKASQLNPIDALHYE"
     gene            complement(35704..36393)
                     /locus_tag="PLW16_04685"
     CDS             complement(35704..36393)
                     /locus_tag="PLW16_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70236.1"
                     /translation="MALIETDRLSKIYPVGETSIRALDEVSVTVQPGEFAAIMGPSGS
                     GKSTFMNVIGCLDEPTSGGYRLEGVDVARRDRDTLAEIRNRKIGFVFQGFNLLPRTSA
                     LENVELPMLYNGIGPKERRRLARESLKMLGLEGRETHHPNQLSGGQQQRVAIARALVN
                     QAPLILADEPTGNLDTKTSVEIMELFVKLNRDAGRTILLVTHEPDIAAFSGRIIRFLD
                     GRVISDEPVKH"
     gene            complement(36510..37727)
                     /locus_tag="PLW16_04690"
     CDS             complement(36510..37727)
                     /locus_tag="PLW16_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB70237.1"
                     /translation="MKKKLVITGVLVAALALGGYLLFQKKGETVRYRTDRVTRGDLSA
                     AVTATGTVSAVTTVTVGTQVSGTIKQIFVDYNSLVRKGQLLAQIDPALPQARVDQARA
                     GLQSAVAGVEKAESALTDAQRTLERNRTLYARNYIARSDLDAAETAHRSAQAQVSVAR
                     AQVAQARATLNAEETSLRYTRILSPVEGTVISRNVDVGQTVAASFQTPTLFSIARDLT
                     QMQIDTSVDEADIGRIRVGQAVRFTVDAYPDLNFTGKVSEVRNAPTTVSNVVTYTVIV
                     KVANPELKLKPGMTANVSIITATRNGILTIPNAALRFKGPAEKTTVGARETGAVAGSP
                     AARTPPGQIMRDLWILSNRKPVRVRVGIGISDGNRTELTAGPLKEGDVVILDVAGAAS
                     PQANVPRPSGFFR"
     gene            complement(37724..39013)
                     /locus_tag="PLW16_04695"
     CDS             complement(37724..39013)
                     /locus_tag="PLW16_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB70238.1"
                     /translation="MRKTIVFLLLMTALLPAAAGADDTIRPGETLTLERCLAIALSRH
                     PDLQAATHTVRAGESRIGQARADYYPQLSGAAGYSRSDPSGAGGAAGRSSNPSDSYSS
                     RLSLSQNLYDFGKTSSRVRIRELERDSSQANFEQVRAQIILGVKQAYWELLKSERDRG
                     VARETVGQFQQHLDRAKGFFDVGTKPKFDVTKAEVDLSGAKLTLLRAENAWRLAQVSL
                     SNAIGLPPEAPEFVIVDSLSVTRAPVALDEAIRLAYDRRPEIRALTVRVQAQEETVNL
                     AKKDYYPFLTGNASYGWGGGDFPLDDGWSVGAQVNVPLFSGYATKYQIEEARATLDAL
                     DANLAALRQAVTLEVKQAWLNLREASDRIDTAALSVRQAEENLELAQGRYATGVGSPI
                     EVTDALVAVSNAKTAHTAALYDYRVAQANLEKATGER"
     gene            39481..40104
                     /locus_tag="PLW16_04700"
     CDS             39481..40104
                     /locus_tag="PLW16_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB70239.1"
                     /translation="MGLLKLLIDDPLTFVLLAIPLLYSIIVHELAHGWVALRMGDPTA
                     KNAGRLTLNPLSHLDPIGTIMLFIVGFGWARPVPVNLGYIRDYRRGLVLVSSAGIVAN
                     MIFAFLAVLLLKWLTPAPQSSVGIFLFYLAQINLILAAFNLIPIPPLDGSKILMGFLP
                     ERMQDALARLEPYGFFIIIGLLFVGALNPLIRFFRWLILVPIGFILS"
     gene            40317..40736
                     /locus_tag="PLW16_04705"
     CDS             40317..40736
                     /locus_tag="PLW16_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB70240.1"
                     /translation="MKSYRKELWFQLPARRGFVNITPEVAACLKESGIVEGLCLVNAM
                     HITASVFINDDEPGLHQDYEQWLEGLAPHEPVSRYRHNRTGEDNGDAHLKRQVMGREV
                     VVAVTNGRLDFGPWEQMFYGEFDGRRRKRVLVKIIGE"
     gene            complement(40764..41282)
                     /locus_tag="PLW16_04710"
     CDS             complement(40764..41282)
                     /locus_tag="PLW16_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB70241.1"
                     /translation="MVKLWRCEICGDAYIGETPPTHCPFCGAHQAFIKEAREAVVTFE
                     VPLTEKDRANAERALQVEVSNATFYACAAEKTDDAEGKLLFKALGKIEAEHAAIWKKV
                     LRLPKTPAGNETCHTENVKNLNESHERETRAIAFYQKAGDESNHERLRQIFAALVEIE
                     TDHLDLSEARLK"
     gene            complement(41365..41595)
                     /locus_tag="PLW16_04715"
     CDS             complement(41365..41595)
                     /locus_tag="PLW16_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HPB70242.1"
                     /translation="MKRKKDRDIEKGYPTAAFVAKLRRLADALEKGDRFAIQIAGERI
                     LVPADAVYTIEHEREGGAEEVEFQITWTRKGR"
BASE COUNT         7050 a        13586 c        13793 g         7296 t
ORIGIN      
        1 tcgatgatgt ccacgcccag gcgttcgagc tgccgggcga tcaggagttt ttcgctcggt
       61 ttcatgctgg ccccgggcga ctgttcaccg tcgcgcaggg tggtgtcgaa aatgtaaatt
      121 ctctgggggt ctgtctctgt catcggtggc gctcctttct cgggatcccg gcggttcgcc
      181 cggtcgatcc ttcctcccgt ccggcggaac ggcgccgcga cgggggcttt ttgacgcaaa
      241 aaaaaggcca cgggctttct cgcccatggc cttcgggatg tttcgattcg cgggggttac
      301 ggttctcctg aaagcgacgg gcgtccgggt atggcaagca ggcaaagcag cgccaggccc
      361 agcagtctga gaatgagaat gctgttcaac atgacccgtt ctttgcccat cccgtgacgc
      421 acgttcgatc cctcgcgttt cttcgttggc ccctccctaa aacagaagcc cggaaaagtc
      481 aaggccgatc tttcgcgtcg ccctccgggc gcggagttca ggcgccgcgg ggcctgtccc
      541 acgcgggaat cagggattga cacgcccggt gtgaagggaa tattcccggg gctgcgcctc
      601 ggcgtctcct ccccgtttag agaacgatgg cgccctgtcc ggccgaggcc acctgccgcg
      661 cgtaacgggc cagataaccc gtgagggggg ccggcggacg gggccgaaat ccctgccgac
      721 gctccgccag catttccgcg gggacctgga ggctcagggt gcgtccgggg atgtcgatgc
      781 ggatgcggtc gccctcctcg atcagggcga tggggccgcc cgccgccgcc tcgggggcga
      841 tgtggccgat ggccgccccg cgtgtgccgc cgctgaagcg cccgtccgtg agcagcgcga
      901 cggtcttgtc cagtcccatg ccggcgatgg ccgcggtcgg gccgagcatc tcgcgcatcc
      961 ccgggccgcc gcggggccct tcgtagcgga tgacgacgat gtcgcccggc cggatcgccc
     1021 cggccaggat ggcggccatg gccgcctcct cgccgtcgaa cacgcgggcg cggccctcgt
     1081 tgacgagcat ttcgggggcg acgccggagg ccttgaccac ggccccctcc ggggccaggt
     1141 tccccttcag gatggcgatc ccccccgtcg cggagaacgg cctctccagg gggcggatga
     1201 cctcccggtc cgccacccgg gcccggcgga gcgtctcgcc cagggtcatg cccgtcaccg
     1261 tcattgcctt caaatccagg agccccgagg gcgccaaggt cttcatgacg ccgggcaccc
     1321 cgccggcccg gtccaggtct tccagatggt gggggccggc gggactcaga cggcagaggt
     1381 tcggcgtctg cgcgctgacg gcattgaaaa ggtcgagatc gagcgtcagc cccgcctcgt
     1441 gcgcgatcgc cggcaggtgg aggaccgtat tggtggaaca gccgagggcc atgtcgatcg
     1501 tcatggcgtt gcggaaggcc tccatccggg cgatccggcg ggggcggagg ccccgcctca
     1561 cgagcgccat catcttgacc cccgccgtct tggccaggcg acgccgggcg gcgtggacgg
     1621 cgggaatcgt gccgtttccc ggcagggcga ggccgatggc ctcggtcagg cagttcatgg
     1681 agttggcggt gaacatcccg gcgcaggagc cacaccccgg gcaggcggcg tcctccaggg
     1741 ccttcaggtc gcccgccttt atccggccgc tccgcagggc cccgaccccc tcgaagacgc
     1801 tgatcaggtc caccggttgc cccccggcat ggccggccag cataggcccg ccgctgacga
     1861 acaggcaggg gaggtccagg cgcagcgcgg ccatgagcat ccccgggacg atcttgtcgc
     1921 agttggggag gaaaacgagg ccgtcgaagg ggtgcgccat cgccatgacc tcgatggagt
     1981 cggcgatgag ttcccggctg gcgagggagt atttcatgcc cgtatgcccc atggcaaggc
     2041 cgtcgcagac gccgatgacg ggaaaggcca tgggcgttcc cccggcggag cggatgcccg
     2101 ccttgacgtc ggcggcgagg aggtccagat ggacgtggcc ggggaccacg tcgctcgcgg
     2161 cgctcaccac gccgatgagc ggacggcgga tctccgggtc ggtgtagccc atcgccttca
     2221 agagcgcccg gtggggggcg cgctccgggc cttttttcat cagatcggat cgcatcgcgt
     2281 ctcgtccctt cgttggcggg ggtctccccg ttttttcatg caaattacac cgcacggggc
     2341 ggccgggaat ctttccccgg tctcattttc cggccgggcg gaggcgtttc tccggccgca
     2401 gcgcgcggac ggcggcgccg gcccgccaca tctcggaagc gcggatcgcg gcgagttcct
     2461 tctccaggcg ctcgcgataa tcgggcgcac cgttgacgtc gagaacgatg gccgtttcct
     2521 cccccgagac gacgctcgcg tatagcgcgt cgaacacggg cgccaccgcc tcccggaacc
     2581 gccccttcca gtccagggcg ccgcgctggg ccgtcgtgct gcagttgccg tacatccagt
     2641 ccatgccgtt ttccgccacg aggcggatca ggctctgggt gagctcctcc accgtttcgt
     2701 tgaacgcctc gctgggggag tggccatggc ggcgcaggag gtcgtactgg gcctccatga
     2761 cccccgcgag acagcccatc aggacgcccc gctccccgac gaggtcgctg tacacctcct
     2821 tttcgaaggt ggtcggaaac aggtagcccg aaccgatggc gatccccagg gccagggtcc
     2881 gttcctcggc ccggccggtg gcgtcctgga agaccgcgta gctcgcgttg atgccgctgc
     2941 cttggaggaa gttggtccgg accgtccgcc cggagccctt gggggcgaca aggatgacgt
     3001 cgatgtccgc cgccggcacg acccccgtcc ggtcccggta ggcgatggaa aagccgtggg
     3061 aaaaataaag ggccttgccc ggcgtcaggt ggggggcgag ggtcggccac agggccttct
     3121 gccccgcgtc ggacaggaga tactggacga cggtgccccg ccgggcggcc tccgcgatgg
     3181 gaaacagggt ctcgccgggg acgaagccgt cggccacggc cttatcccag gatccggtcc
     3241 cctcccgctg gccgacgatg acgcggacgc cgttgtcccg caggttcagg gcctgtccgg
     3301 gcccctgcac cccgtagccg acgacggcca ccgtctcgcc cgcgagggcc cgccgggccc
     3361 gctccagggt gaattcctcc gctgtggtca cctcttccca ggtgccgccg aaattgatcc
     3421 ttgccatacc ttcctcctcg ttgtccgttg ttgttttcat gatccgcgat ccccacggtt
     3481 cctgtcctcc ggggcatgac gaatcacgcc gtatgatctg tcccacgact ccaccctgcg
     3541 accggtccgg gaaggccggg ttgtcttccg aaccggcaac ccccgcccgt gcctgccccg
     3601 gcggacggcc ggataccggc gtcaggacgg ccgccggggc agggcaatcg tcccggtccg
     3661 gtttaccgcc cggatcccga aagggcggaa gcacgccagg atgccgccca ggtgctccgg
     3721 atcgccggcc gcctcgatca ggcagattcc ctccgtcaaa agcaggatcc gcccctggca
     3781 cagctcgatc gcccgcagga tctccgcgcg ctcctcttcc cggaacgtca cgtggaggag
     3841 catcagctcc cggcgcagaa agggcgtccc ggtgtaatcg gcgacctcga tgcagtcgac
     3901 gagcttgtcc aaatgcttcc ggatcttgtc cgtgaaatcc cgctcggccc ggctggtcag
     3961 ggtgatccgg gagacggcgg ggtccgtcgt ttccgccacg cagagactgg cgatgttgta
     4021 gcccaggcgc cccaggatcc cggccacccg cgacaggacg ccgggccggt tccgcaccag
     4081 gatggagagg atctgttctt cgtgaatttc aggggggggc tccatgttcg ggatctcctt
     4141 ccgttgtgtt ccggccgtgc gtccggcctc cgtctgtgga cttcgtcgcg ccgcttttcc
     4201 cttcgcgggt tcaggccgtt ctgccgtaga tcatctccat gttggaacgg cccggaggga
     4261 tcagggggta gacgtagcgc tccgggtcga tgaccgcttc caggagaaag gggccttcgg
     4321 accggcggag ccggtcgagg ccctccgccg tgtcgccctc catgtccgtc cgggcggacg
     4381 ggatgccgaa gccccgggcc acggccgtca aatcgacgtg gccgcccagg gcgatggtgt
     4441 tcagccgtcc gtcgaagagc agatcctgga gctggcggat catgcccaga tggccgttgt
     4501 tgatgacgat gatcttgacg gggagccggt aatagccgat cgtcgccagt tcctggatgt
     4561 tcatgtagca ccccccgtcg ccggtgatgg ccacgacggc gtggtccggc cgggcgatct
     4621 gcgccccgac ggccgcgggg atgctgaacc ccatggtccc catgccgcca ctcgtcaaga
     4681 tgctgcgggg cgtccggggc tgccagttgc ggagcgtcca gatctggttc aggccgacgt
     4741 cagcgacggc aatggcctgc gggggcaggg cgcactgcac ctggcggatg aacgccccgg
     4801 cggggccgtc gcccgagccc ttccggccct cccggaggac cgcccgttcc gcctcgatcc
     4861 gctccatcca tcccgacgcc tcccggcgtt cgacccgcgg caggaggccg cggaggacgt
     4921 cccggatgtc tccgaggatg gggcagtcgg ccttcaggtt cttcccgatc gaggtggggt
     4981 cgatgtcgat ctgggcgatg gttgcgagcg gggcaaactc ctcggttttc gaggtgctcc
     5041 ggtcggagaa ccgggcgccg acggcgagga tgaaatccgc ctggtgaatg atccggttgg
     5101 ccagttcgtg accgtgcgtg ccgatgaatc caaggttgta tccgttgccc gacgcgacgg
     5161 cccccagtcc catcatggtc gtggcgaagg ggatgcgggc cttggccacg agcgcctcca
     5221 gctccccgca ggcgccgctc agcttggcgc cgccgccgat caggatgacg ggccgttcgc
     5281 tgcggttgag cagccgggcg agctcctcca ggggcgccgc gtccggcgcc gtcggggccg
     5341 gacgcgtttc cggacgggag ggcgccgcgt caccccctgc gggggcggcg aggatgtccc
     5401 ggggcaggtc gaccaggacc gggccggggc ggccgccgcg ggcgagatcg aacgcctccc
     5461 gcagggtgtc ggccatgtgc cgcacgtcca gcacctggaa gctgtgcttg gtgatcggga
     5521 gcgtgatgcc gatgatgtcg gtctcctgga aggcgtcccg gcccagcagg ttcgtgttca
     5581 cctgggccgt cagggcgacc agggggatcg aatccatgaa ggccgtggcg atgccggtga
     5641 cgaggttggt cgcccccggt ccggacgtgg cgaggcagac gcccaccttg ccggaggccc
     5701 gggcgtagcc gtcggcggcg tgggcggcgg cctgctcgtg gcgcatcagg tagtggcgga
     5761 tcgccgttgc ctggatgcgg tcgtgaacgg gcagcgtgtt ggcgccggga tagccgaaaa
     5821 tcgcctccac cttttcctgg cggaggctgt cgagcaggat gtctgcacca atcaggggac
     5881 tcatcggttt gaaactcctt tccgtgtgta ggctcatcgg cggagcgcgt ccggcgacga
     5941 gagatatcga aaataaaaaa gccgcattcg ctggcgattg cggcttttcg gacataaaaa
     6001 aagccgcggg tcctgaaggg cccgcggctc ggatcggtta cggcatcttc agacgcacga
     6061 acgagcgacg gtccccgtct ctacgacgac tacagcgacg cggctcgata cgacctggag
     6121 ggttttcatg ttgacggaca tcggttctgc cttgacctct cgaaggatgg gtggcgctct
     6181 atacccggat agaaaaattg tcaagcgttt tttaaaagaa gccttcctgt tcatcggccg
     6241 gaccgtaggt cggtacctcc tcgggcgtcg tcccttccag gaagctctcg tacagacctc
     6301 cctgggtccc gtccgggagg gatgcccccg tattcggatc cactttgacg aacgaaatgc
     6361 cttcgggaac ggggaacacc tccaccggcg ttccctgcac ggccttttcc ataaagtaaa
     6421 gccagatggg cgccgccgcc cggccgccca cctcctgcct tcccaaagaa cgctcctgat
     6481 cgaaccccac ccagaccccc gtgacgaagg aaggcgtgaa ccccatgaac caggcgtcgc
     6541 gcatatcgtt cgtcgtgccg gtcttggcgg caacggggcg accgatgctc cgcacccgcg
     6601 cgcccgtccc ctgctccacg acgtcctgca tgacgtaggc ggacatgaag gcgatccggg
     6661 ggtcgatgac ctgttccagc atgggctggt tctcctcgaa gacttggccg gtgcggtcca
     6721 cgatcttctt gatgaacagg ggcgtggccc gctgcccctg attggccagc acgccgtagg
     6781 cccggaccat ttccaggagg gtcaccccgg agctgccgag ggcgagggtc aggttgcgcg
     6841 cgaggggtga ggcgatgccc atgttggcgg cgtaccccgc ggcgtaatcc acgccgatct
     6901 ccttcaggac cttgatcgtg acgacgttcc gggaatggac gagggcgtgg cgcagggtca
     6961 tcggtcccaa gaacgttccg tcgaagtttt tcggttgcca cacgccgttg ggttcggagg
     7021 gggccgcgaa ggagatcggg gaatccacca tgatcgtgga gggcgttagc cccttgtcga
     7081 aggccgccgt gtagatgaac ggtttgaagg ccgatccggg ctggcgccgc gactgggtcg
     7141 cccggttgaa ttcgctgcgg gagaaatcac gaccgccgac catggcccgg acggcgccgg
     7201 tccgcgcgtc catgcacaac agggccccct ggaccagccc tttcgcatag ccctggcgcc
     7261 tctccatttc ctgaaggccc cgctccacgg cgtctcgggc ggccttttgc atgggcaggt
     7321 tgagcgtcgt gtagacctcg aggccctcct tgtacaggac gtccgagccg tacttctcca
     7381 tgatgtagcg gcggacatgc tcgacgaagt aggcggccac cttgtctttg ggccgcacgg
     7441 acctgaggcg gagcggcgtg gccagggccc tttccttttc cgcggccgtg atgtagccgt
     7501 catccagcat gcgctgcagg acgtaggcct gccgctgcag ggaccggtcg gggtggagga
     7561 acggcgaaaa gttgttcggg gccttcggga gaccggccag gagggcgact tcggaaaggg
     7621 tcaggtggcg ggcgctcttg ccgaaatacc cctgggaggc ggcttcgatc ccgtaggtgc
     7681 cgtgacccag atagatgtag ttcagataga gattgaggat ctcttccttg gtcaggtagc
     7741 ggtcgatctt gtaggccagg agcgcctcct tgatcttgcg ccggtagctg cgctccgggg
     7801 aaaggtagag ggctttggcg acctgctggg tgatcgtgct cccgccctgg acgatgtgac
     7861 cggcggccag gttcttgaag aaggcccggg tgatgctctg gtaatccagg ccccggtgct
     7921 cgaagaaccg cgtatcctcc gcggcgacga aggcctggat gacgtagcgg gggcactcgg
     7981 aaagcttgat gaccttccgg tcttcgagga agaactcgtc gatgagttcg ttgttgtcgt
     8041 catagacccg ggtggcgatg ctcggccgga aatccttcag cgcggcgatg ctggggagtt
     8101 ccatgagcag aaggtagaag acgaggctgg tccccagaag gacgaccccg agcgccccca
     8161 gaatgatcag gagggaggcg tatccccgct ggccgtcagc cgttccccgc gaggtcttcg
     8221 agccgcggcc gccggtcatg acggcgactc cttcccgttt tcgattccgg aatccgcggc
     8281 cttttttctc tcggcgacct tcgcgaccaa gatgcccaac tcgtaaagga ccatgagcgg
     8341 cagggccatg atgatttggc tgacggcatc cggcgacggc gtcaggatgg ccgaggcgat
     8401 gaagatcatc aggatcgcgt agcgccgctg tttgcggagt atccggacat tcaccacgcc
     8461 caccttggtg aggaagtaga tgaagaccgg cagctcgaag gcgagtccga acgcgagcag
     8521 gaacttcagg gccagggaga ggtattcccg gaaggagatc atcggcctga gcatctccgt
     8581 cgtgaagctg acgaaaaagg cgaaggccgg aggcagggcg atgaagtacc cgaagaggac
     8641 gccccccgtg aagaggagcg tcgaggagag gatgaaggcc agaccgtagc gccgctcttc
     8701 acgatagaga ccgggggcga cgaacttcca cacctgatag agcgtgtagg ggctggtgag
     8761 gacgagggag gcgaaaaagg cgatcttcat gtagatgaag aaggcctcgg gcaggccggt
     8821 gaacaccatg tggctgtttt tcggaaggac ggcgacgagc gggcgcgtga tgaccacaaa
     8881 cagccagtcc ttgaagacgt agcagacgcc gaaccccacg cccagggcga tcagaatccg
     8941 gacgagccgg gtgcggagtt cgtctagatg ggaggtcagg ggcatcttgt cttcggcggg
     9001 tttttccatg gatgaaatcg acgggcggcg ctccttcggg cgataaatcg ggaatgaccg
     9061 gcgggtcagg tcgttggggc ggccggggtg tcgtccggcg gaacggtacc ggctttctca
     9121 tcggcgggtt cggagtcggt gtcggatttt ccgtacagga gggaatcctt caggccggcg
     9181 cccttctcct gctcctccgg cttctcctcg tcctcccgca gggtgtcctt gagggtatcg
     9241 gtgacgccgt cggtggcccg cctgaattcc tgcaggccct tcccgatgga tttggccaga
     9301 tcgggcaggc gcttgggccc gatcacaagc agggccacaa tcaggatcac aatgagctcg
     9361 ggagtgccga tgccgaacat ggggtcttct cttttccgtt taacgtgcac gcgaatatag
     9421 cttttatgac aagaggttgt caaacgtttt cccgcctggg gccggcgccc gaaacccgtt
     9481 gctttttcga ggggggtgtg ataggaaaaa ccccttttaa aaaacctatt tgtcgggtcg
     9541 ggagacggcg atgtttcgca aaacgggggt ggtcaaggac aggcgttatc tggatcatgg
     9601 ccgggatatc gcccatcccg aatcctacaa gcggctgcag gcgctctatg aaatcctcga
     9661 agcccccaac atgatgggga agtttaccga ggtcgagccc cgccatgcca cctacgcgga
     9721 gctggagatg atccatgaac ggtcctacat cgatttcgtg gccgacacgg ccgggaagga
     9781 gatgtgctcc ctcgatccgg acacgattac gaccgccgaa acctacgacg tggcgcgcct
     9841 ggccgtgggc ggcctctgcc gggccgtgga cgccgtcatg gccggggagg tggagaacgc
     9901 cttcgccctg atccggccgc ccggccatca cgccgaggcg gacgaggcgg ccggcttctg
     9961 catcttcaac aacgtggcca tcgcggcgaa gtacgccctg gagaagcaca ggctcaagcg
    10021 ggtcctgatc gtggactggg accttcacca cggcaacggc acccagaacg ccttctacgc
    10081 cgatcgccgg gtgctgtact gctccacgca cgagtcgccc ggctatcccg ggacgggcgc
    10141 cctcgaggag atcggacagg ggaagggcct cgggtacacg gtcaacattc ccctgaagtc
    10201 cggggcgggg accgcccact acgtgaaggt cttccgccgc ctgatcgtcc cggtcgcgat
    10261 gacgttccgg cccgacctga tcctggtttc ggcgggattc gacatcttca gcggggaccc
    10321 gctcggcggg atgaaggtga cgccccgcgg cttctcttac ctgacacgga tcctgatgga
    10381 cgtggccgac gcctgttcga acgggcgcat cgtcctcagc ctcgaaggcg gctacagcgt
    10441 cgaaggcctc acgatgtccg tcacggcggt catcaaggag ctgagcgagg ccacacacgc
    10501 gacggaggac gaactccggg acatggaagc gtccgccgac acccgccagg acgccgtcat
    10561 ccgggcggtc atgaatcagt tgaatccgtt ctggaaggtg tattgaaggc cgaggtagag
    10621 gggcagggtt ccgtcgcccc ggtcttgcgc cccccgtcct gattttttga agtaaacttc
    10681 acgcgaaaat tgcccgttgg catggatcgt gtgtacatca aggcaagcga cagccttttt
    10741 ttgaaggaga tttttcatga agattacccg agcggaagtg gaacacgtcg cccatctggc
    10801 caggctcgag ttcgacgagg cggagacgga gaaattcacc tcccagctga atgacatcct
    10861 gctgtacatg gacaagctca accaggtcga aacccgggac gtcgcgccca tgacccatgc
    10921 catcgcgcgg accaacgcct tccgggagga ccgcgtccgg aaatcgctcc cccttgagca
    10981 ggccctcgcc aacgccccgg acccgcgggg cgagttcttc cgggtgccca aggtcatcga
    11041 ataagcgggc cgcctgcacc ggaaggggac gaatatccac gggcgaacgg acgggcgccc
    11101 ctgatcgggg cttcggacgg cgtttcggcg aacatcgagg aggacatgga actttacacg
    11161 ttgacgattc acgaactgca ggacaggctc agggccggag agacgaccgc agaggcgctc
    11221 acccgcgccg tcttcaaacg catccgggcc gtcgagtccg acgtccatgc ttacatcacc
    11281 ctgctggagg agccggccct cgcccaagcc gaccgggccg accgggcgat ccgggacggg
    11341 gacctccggg cgctgaccgg gatccccatc gcgctcaagg atgtcttctg cacccggggt
    11401 ttccggacga cgtgcgggtc ccgcatcctc gagaacttcg ttccccccta cgacgccacg
    11461 gtcgtggaaa agctcgccgc cgccggcgcc gtctttacgg gcaagacgaa catggacgag
    11521 ttcgccatgg gctcctccac ggagacgtcg tatttcgggc tgacgcggaa cccctgggac
    11581 ctcgaccgga tccccggcgg ttcgagcggc ggatcggcgg cggcggtcgc ggccgacgag
    11641 tgcatcgcgg cgctgggctc cgatacgggc ggttcgatcc gccagccggc ggccctgtgc
    11701 ggcgttgtcg gactcaagcc gacctacggg cgcgtctccc gctacggact ggtcgccttc
    11761 gcctcgtcgc tcgaccagat cggcccgttc acgaaggacg tcgaagacgc ggccatcctg
    11821 atgaacgtca tcgccggcta cgacccgcgc gaatccacgt ccgttccgga ggacgtgccc
    11881 gactaccggg cgtttctcgg tcgcggcatc gacggctgga ccgtcgggat cccgaaggag
    11941 tacttcgtgg ccgggctcga cccggaggtg gagcgggccg tccgcgacgc gatcggcgtg
    12001 atcgaaggcc tgggggcgcg ctgcgtcgag gtttcccttc cccacacgga acactgcctg
    12061 gcggtgtact acatcgtcgc cccggcggag gcgtcgtcga atctggcccg ctacgacggc
    12121 gtccggtacg gctaccggtc gccggacggc cgggatttga tggagatgtt caagaagtcc
    12181 cgctccgccg gcttcggcgc cgaggtgaag cggcggatca tgctcgggac ctacgccttg
    12241 tcctcgggct attacgatgc ctactacaag aaggcctccc aggtccgcgc gctgatcaaa
    12301 aatgatttcg acacggcctt ccggacctgc gacgccctcg tgacgcccac gacgccgacg
    12361 cccgccttcc ggatcggcga gaagacggac gacccgctgc agatgtacct ctcggacatc
    12421 ctgacgatct cgacaaacct ggccggtatc cccggtatct ccgtcccctg cggctatacc
    12481 gccgccggcc tgcccgtcgg cgtccagttc cagacggcgc atttcgcgga gggcaagctc
    12541 ttccggatcg ccgccgcctt cgagaagcat gcgaatttca ccaaaaggag accgaagctc
    12601 tgatggaata cgaaccggtg atcgggctgg aggtccacgc ccagctcctg acggatacaa
    12661 aaatcttctg cggctgcacc acccagttcg gcgccgcccc caacacccac acctgtccgg
    12721 tctgcctggg catgccgggc gtcctgcccg tcctcaaccg gaaggtcgtg gcgttcgcga
    12781 tgaagatggc cctggcgacg gactgccgga tcaacccggc gtgcagatac gcccggaaga
    12841 actattttta ccccgacctt cccaaggggt accagatctc ccagttcgcc gagccgctcg
    12901 ccgaattcgg ccatgtggac atcgaatcgc acggggggaa gaagcggatc ggaatcaccc
    12961 ggatccacat ggaggaggac gccgggaagc tcatccacga cgagtacaac ccctggagct
    13021 acgtcgacct gaaccgcacc ggcgtgccgc tcatcgagat cgtgagcgaa ccggacatgc
    13081 ggagcgccga ggaggcagcg gcctatctga agcgcctcca cgaaaccctc gtctacctcg
    13141 agatctgcga cgggaacatg gaggaaggct ccttccgctg cgacgccaac atctcgctcc
    13201 gcccgaaggg gacggagatc ttcggcacgc ggacggaact caagaacatg aactccttcc
    13261 ggaacgtcca gcgcgccctg gactacgaga tcaagcgcca gcagtacgtc ctcgaggggg
    13321 gcggcgaggt cgttcaggag acgcggctgt gggacgatac cgcgggggtg acccactcga
    13381 tgcgcgggaa ggaggaggcc cacgactatc gctactttcc ggatcccgac ctggtccagg
    13441 tcgaggtcga cgaaacctgg atcgcacaga tccgcaacga cctgcccgag ctgccccagg
    13501 agaagcggga gcgcttcctc aaagactacg gcatccccgc ctacgacgcc ggcgtgctca
    13561 cgtccagccg ggcgctcgcg gactactacg aggcggtcgt ccgcctttcc ggcgagccga
    13621 agacggcgag caactgggtc atgggggagg tcctccggtt tttgaatgag gaaaagcggg
    13681 acatccggaa ctgccccatc cggcccgagg ccttggcggc catgatcgcg ctgatcaagg
    13741 acgggacgat cagcggcaag atcgccaagg acgtcgtcga ggagatgtac cggacgggca
    13801 agttcccgga agacatcgtc cgggaaaagg ggatggtcca gatcaccgac gagggcgccc
    13861 tgatcccgat catcgacaag atcctggcca agcatcccgg tcaactcgaa cagtaccgcg
    13921 ccggcaagga caagctattc ggcttcttcg tcggtcaggt catgaaggcg acgcggggga
    13981 aggccagccc ccagttgatc aacgacctcc tgcaaaggag gcttgcgggc gaccggtagc
    14041 ggcgtgcggg aggcggcgtc cgcatgaccc ccttcattcc cgcttcgccc gcgccgagcc
    14101 gatggacgga catggaagac gcgatccaca ccctattctg gcgggacgat accgtggttt
    14161 tgctggacca gaaggcgctc ccccgcgagg agcgctacgt gacctgcacc gattaccgcg
    14221 aggtcgtcgc cgcgatccgc gatctcaccg tccggggcgc gccggccatc ggtgtcgccg
    14281 ccgccctggg gatcgctctg ggcgttcgtc atctcgacgc cgcctccata gacgcctttc
    14341 gggaggcctt ctggaacatc tgcggggact tcgccgccgc ccgtcccacg gcccggaacc
    14401 tgttctgggc gatcgagcgg atgcagcgcc gcttcgacgc ggcgctggtg gatgcggcag
    14461 gatccgccgt ccctcccgga tcggggtccc tccctcgggg gagaggggaa cgggaccgtc
    14521 tgcgggcggt ttccgacgcc ctcatcgccg aggcgatccg gatcggggaa gaggacctcg
    14581 ccatcaaccg ccggctcggt cggcacggca gcgtcctcat caaggacggc gcccggatcc
    14641 tcacgcactg caacgccggg gccctggcaa cggccggtta cgggacggcc ctgggggtga
    14701 tccgggcggc ccgggaggcg ggcaaggcgc tccacgtcta tgtcgacgaa acgcgccccg
    14761 tcctccaggg ggcgaggctg acggcctggg agctgacgaa agaaggggtc ccctgtacgc
    14821 tcatcacgga caacatggcc ggcttcctga tgcagcgggg gaagatcgac ctcgtgatcg
    14881 tcggggcgga ccggatcgcg gccaacggcg acacggccaa caagatcgga acctatgccc
    14941 ttgccgtcct ggcgcacgcg cacgggcttc cgttctacgt ggccgcgccg tggtccacgg
    15001 tcgatttttc gctggcggac ggtacgggaa tccccatcga ggagcgggac ccgggggaag
    15061 tggtgcgctt cgcgggcgtc gccaccgccc cggaggggat cgccgtctac aaccctgcat
    15121 tcgatgtgac gccccaccgc tacatcacgg cgatcatcac ggaggacggc gttttgacgc
    15181 cgcccctgcg gcggccgcac acccgggacg ggcggtagcc gggcggcgat tcgaaacggg
    15241 ccggcgatgc cgcccggcgg tccggaaggg aacgttacaa cgcgggagaa atcttggtgg
    15301 cacaggcatt tcagaccagg gatgcggaca cggggggcga cgcgggcgat ttcgtccgga
    15361 cgttcctgtt cggatccgcc ggtgtccggc gtgaggggcg aacgctccgc tccgtcatcg
    15421 tgatggacga aaaaaaacag gggtgggtgg ggatccctca cggggggatc gccatgggcg
    15481 ccgtggccga cctcgccggg gacctgacgg aggagtgccc gggtccgttc ggcccctacc
    15541 cctatgccat cgactaccgc atgggtggtg cgagcgcccg gctgggggat acactcgcga
    15601 tcgaggtgac cgccacggcg agcggcgccc ggggatcgat cgtcaaagac ccccgaacgc
    15661 ccccctacct gtcggcgtcg gtccgcttcg ggggcgatgc cccatccacc gagcgacgcg
    15721 tgccggccgg tctgcccgcc cggatcgacg cgtgggccgg ccggctgctt gacctgccgt
    15781 tctaccggaa ctgctttgtt tgcgggatcg cccgccggca aaccggtctc ggccgccgct
    15841 tctacgttcc ggacgtgccg gacagcccgg cgacggttgt ctccccgatc ggatgtctgg
    15901 gcgacgacgg ggcggccttc tggcggtttc agcgccaggg gcgcctgcac cccctccccc
    15961 tggtcgctct gctggacgag atcatcggct ggggcggctt tctcctgacc gcgagcggcg
    16021 gcgtgaccgt gagggcgagc tacacgttct atcgcgacat cgccgccgga gaaccgctcc
    16081 tcgttttcgg aaggggagag aaggtccggg gcaacgcccg ctcccgattg ctgttctggg
    16141 cctccggcgg cgcggcggcg gttcgttccg atggcggcct cgaaccggtg gcggcggcct
    16201 ccggccagta tctgggcatc ggggccctga cggagcagat gaagcgggaa ctgcttccgg
    16261 ccgacctgac ccgccgggcc ttccaactcg ccggcgccga cgtgtgagcg cactcgccgt
    16321 ggcgtccgcc gattcggtcc ttgcccgcgg gccggttttt ggtgtaatgg gtatctcatg
    16381 aaaaaactgt ttctcttcga tttcgatggg gtgctggtgg attcgctgga cttttacgtt
    16441 caggccgtgg gggcgtgcct gagggagatc gggacgccga tcgtccagac ccgggaggac
    16501 tacctcgccc tgttcgaggg gaatttctac gaggcgatgg ccgaacgggg tgtcgatctc
    16561 gcggccttcg cccgggcggc ccacgagatt ctcccccgct tcaattacga cgccttgagc
    16621 ccctttcccg gcatcgcgcc cgtcctggag acgctccacc gggatcatct cctcgccgtg
    16681 atctcctcga acgggtcccc gacgatccgg gcgatgctca agcgatttca atacgacggc
    16741 tactttcggg acatctttgg atccgacttc ctcttcagca aggtggacaa gatcaaccac
    16801 gccctggagg cgttcgggat ggcggcggac cggaccttct acatcgggga caccgtcggc
    16861 gacgtcctcg aggcgcggga ggccggcgta aagaccgtcg ccgtcgcctg gggctggcac
    16921 ggccgtgaaa agctggctga cgccgggccc gaccatctgt tcgaccggcc ggaggaactg
    16981 ctcggcctgt gaggccgtcg ggggagggtc atcagacatg aaacgattgt atctcgcgac
    17041 ggacgatcgg aaaatcggcg gggtgtgcgg cggcattgcc gagtacgtcg accgcgatcc
    17101 gaccctgatc cgggtccttt tcctcttgct cgtcatcttc tctttcgggt ttggtatcct
    17161 cgcctatctc gggatgtggc tcgtcattcc gaaaaaaccg gcatccacgg ggggcggcgc
    17221 acggggcgac ggttgatgcg ggcaggggag gttcgtgttg gatggggagt caaccatgga
    17281 ttatgagacg cttctgaaac ggcgacgggc catccgggac ttccaggacc gggaggtgcc
    17341 cctgcccgtg ctggaggaga tcctccggga gacctgcctc gccccgacgg cgagcaatcg
    17401 ccagccctgc aagttcatcg tcgtgcgcga ccgggaacgg atgcggaggc tctcggatgt
    17461 gagccggagg acgctgcttg ccgatctcga ccggaatccc gactcgcccc tgaaggcgta
    17521 cgaagcctac ctgcgggacg acggggtcaa cgtcttctac aacgccccct gcctcgtgtt
    17581 cattctcgga ccggacgacg tacggtccct ggatgtcgat tgcggattga cggcggccta
    17641 cttcatgttc gcggcgacgg cccggggcct ggggacctgc tggatcggcc tgggggcgca
    17701 cctgaaggac ccgaaggtcc tcgcggaact gggcgtgccc gaaggctgcc gcatcgtggc
    17761 cccgctcatt ctcggctatc cgaccgccgt tcctccggcg tccgagcggc acgccccgga
    17821 aatcgtcaag gtcatctgaa gcggacggaa cggcgggagg tcctcctttg actgggaacc
    17881 ccaacgatga agacctgagg gaaagaatca gggccctcga ggaagaatcc gcccagcgcc
    17941 ggcgggtgga agaggcgctg cgggaaagtg aacgaagata caggttcctg gccgaaagaa
    18001 tcaacgacat cctgtggatt atggacctgg acctgcggac ggtctatgtc accccctccg
    18061 tccagaccgt cctgggattt accccggagg aacgcctgcg ccagaacgtc gaggagcagt
    18121 tggcgccgga ctcgctctcg acggcgctgg agaccctgtc ccgggaaatt gccttcgaaa
    18181 aacagcacgg cgccgacccc gataggagtg tgaccctcac cctggaattc tatcaccggg
    18241 acggctcgac ccgctggctg gagacgatca ttagcgggat ccgggacgat cggggcgtcc
    18301 tgacgcgact ctacggggtg tccagggacg tgacccggcg cagggccgcg gaggaggccc
    18361 tgaagaccgc gaataaacag cttgaagagg ccaaccggtc cctggggctg gcctatgcct
    18421 ggatgcggga caggaaggac cagctgaagc agcagttgtt ccgggaagaa atggggattc
    18481 tgctgaacaa cgaaggcttg atcgaaggcc tgtcggaaaa ggtcatggag catctgggac
    18541 ggtcgcggga caggctgatc ggacacccgg tgagggacct gatcaaggaa gaataccggg
    18601 aggacttctc ccgggagttt cggcaggcct ggatggggcc gtcccaggag gtctttgtcg
    18661 aatttctcga tacgaaggac cggtcgaaga ttttcgatgt caagctgacg cggctgaccc
    18721 tttccggctc gcggctcgtg ctgatgacgc tccgttgagc gttgcggctc gcgcgatgga
    18781 tgttttgccc cacatcttct gcgaaggaga caatcgatga aaaaaaccgt tatggccatt
    18841 ttcacgtccc tgatgatgct gatggccgtc ccggcggtgg ccgagaattt tacggtccag
    18901 ggggacatgg cctccaccat ccactacgag cttcagcatc agatcgtggc cggcgattcc
    18961 atgagaaaat tgatgctgag cttcgtggtg cccgaaagct tcgactctcc gacatacaag
    19021 cagaagatca cccgttttaa agtccggttt tcaccggagg ccgaggacga aaccaccacg
    19081 accaacgccc gcggtaacaa gattatccag gcgacctggt acaccgttcc ccgggtggtt
    19141 gacgccacga tcgcctttga cgcgcaaacc acgaccggct tgaaccccat ccaatccacc
    19201 gcgcctttcc cggccgtgtc cacacccgag gatctgaagg attatctgca ggcctccgag
    19261 ctggtgcaaa ccggggatcc cgccattcgg gcgctggcgc tcgaactgac ccagggcgta
    19321 aaaacgcagt tcgatgccgt gcagaaggtc gtgagctggg tcgtggatca cgtccggtac
    19381 gtcaatcccc cggtgcagta cgacgcgctt tattctttgc ggaccggcaa gggcaactgc
    19441 cagaactact cgcacctgac ggccgcgctg ttgcgcggcg tgggggtgcc ggtgcggatc
    19501 gtcaacggcg tcacgatgaa tcagccgttc gacgtgtcct gggagagggg aacgctgacc
    19561 ttcaagatgg ggcagggccg gcattcctgg gtggaagtct ggttcccgga tctgggttgg
    19621 gttccctacg atccgcagaa catgcagttt tttatttcca accggttcgt ccgcattgaa
    19681 gtcggcgtgg ataacaacga gaccaaaaat gacggtctgg tgcgctgggc gcagagttcc
    19741 ggggctcgca ccaaaccgac gctgcaggaa accatcggcg ggaacttcct ggctgattcg
    19801 attcaggttt cagccaagcg gcagaattac ggccccaaga atctgctttt gggccccaat
    19861 gtcctggcca gattggagga ggccgccgtt cagccggcgc ctgagcctgc gccggcccct
    19921 ccgatccccg tgccgcccgt gcctgcgcct gaacccatgc cgcccgcacc ggcacctgag
    19981 cctgtaccga aaccggcgcc cgctcccccc gttcccgtgc cgcccgtgcc ggaacccgtt
    20041 cccgcgccgc ctgagcctgc gcctgaaccc gcgccggccc ctccgatccc cgtgccgccc
    20101 gcaccggaac cggcgcccgc ccccccgatc cccgagccgc ccgtgccggc gcccgtgtct
    20161 cccaagccgt tacgctatga caagcccttt gttttcggca atctggaatt tccggaggac
    20221 gtcgattttg cgtttccccg tgtgacccag gccaaaggcg ggggttcgtt tgaaatgtcc
    20281 cgcaattttc tggtggaaac ggcggaattc gtcacccacg gcgtgacgca gtatgcccag
    20341 gtggtcgaat tggcgaaacc ggcgaccctc catgaaatcg cgctggcatt gcacaacttt
    20401 gggggagacg gtcatctctg ggtggatgtg ttcaaggatc aggacggcca gcccggcgag
    20461 atcctgtgca cgacgcaaat ggtcgctctc gaggagattt ccggccgtcc cggttatcga
    20521 tgggtgacct tcaccttccc ggatcgggaa cggccggttt tggcggcggg gaattactgg
    20581 attgccctcg gtttttcggg gaagccggtc gtcaactggt tttacacgta cggcaaaccc
    20641 gtcggacccg tttacgggac ccgctataaa agcattttcg cgaaggaatg gagcggcgcg
    20701 ctcaattatg aattcaacta caaggtcgtc gggatggcca cggaatagcc gtttgccgat
    20761 cgccggacct ttcgccggaa accggacggg gccggaaggc gcgctgccgc atggcggatg
    20821 atccggtcgg gcgtcgtttc tacaggtcgt agagggtttc gtccacggcg ggcctggatt
    20881 tgcggccgcc ttttctgcct ttcgcctcca gcaggaacgg ctcttccccc tcgagcagat
    20941 cgcccatctc cgcctcgatc ttttccgggt cctcgccccg ctccatccgg ctcaaggcct
    21001 cctccatccc cgccccgagc ttgagccccg tggcgtcgct cagcctccgc atgagggccg
    21061 ccgcctgccg gggatcgtcc tcgttgattc tgtccgcctc gccggccagc atcgccatcg
    21121 ccttttccat cttcgcctcg tcgatgggcg gcatatcgtc cgcgggcggc tcgtccttgc
    21181 cgccggagag cttggcgaag acggacatct gccgcttgag gcggacgcgc ttgcacctcg
    21241 gacaggtcgg gatcttgtcc gtattgacgc tcctggagaa gaagttgtag atcgtgttgc
    21301 acttcgcgca gtaaaactcg tagatcggca tgtccctcaa ccctcttggc gtattcgaaa
    21361 caggtccggc gcgcggcggg acgacgatgg aaaggcgcct ggtcgtcatc cgtccggcag
    21421 gggataatct aaccactccg gcggaaaaat caaccctgcg gggcgcattt tccctttctc
    21481 agacgccggt caggaggacc agacggtagg cgatcacgcc gcccgcgtag gacagggcga
    21541 acatgaacgc gagggacgcg gcaaaccacc ggttgtcggc cagttcccgg cgcatcacgg
    21601 ccaccgtggc cgcgcagggg atgaagagca tcagcacgac gagaaaggcc gctgccgagg
    21661 catggctgat gatccggggc agcatcgcga ccaggccctg atctccgacg gcatagagga
    21721 cccccagcgt ggccacggcg ttttccttgg cgacgatact ggtgagcagg gccgtcacca
    21781 tcttccagtc gagtcccagt ggccggccca gcggctccag gagacgcccg aagcctgcga
    21841 gcaggctcgt ttccacctgg ccgtacggca ggtacgagaa gagccagacg gcgacggaca
    21901 cggcgaggat gatcgttccc gccttgcgga caaaggccgc ggtacgcatc cagaccaccg
    21961 tgccgatcgt ccggatgtcc ggacggtggt acaggggaag ctccatgatg aagggggttg
    22021 cctcgccctt cagaaagaac cggctgatca ccatgcccgt gacccccagg agcaacaggt
    22081 tgagggccag cagtcccgtg gcccaggata tcgcctgcga tccgaacatc gccccggtga
    22141 cgagggtgat caccgccagg cgcgccgtgc aggggatgaa gggggcgaga aagatcgtca
    22201 atagtcgttc cttcttcgcc tccacgatgc gggcccccat gacggacggc acgttgcacc
    22261 cgaagcccag gcaaaggggc aggaagcttt tcccgtgcag gccgatcagg tgcatgaacc
    22321 ggtccatgac aaaggatgcc cgcgccatgt agcccacgtc ttccagaaac gccatgacgg
    22381 caaagaaaat gatgaggatc ggcaggaagg tcagcaccga accgacgccg ccgaccacgc
    22441 cgtcgatcag gacgccgccc agccagtccg gcagcggtgc cagggcggga cgcagcacgc
    22501 cggccgcaga gccgatgagg cgctccagcc aggcctgcag ggggaagccg acccggtagg
    22561 tgaaggcaaa gacgagcgcc aggatggcga agaggatggg gatgccgaac aggggacgcg
    22621 tcaggacgtg gtcgatccga tcggtcgtca gaacctcgcc cagccggtgg cgcgagaccg
    22681 ccgcccgggt gacggactcg atccactcgt accggccggc caccacggcg tgcaacgaat
    22741 cctcatgccg gacgaggagg gcctggatgg ggttccaaac cgcctccggg atgacgccct
    22801 ccatgatctt cgtcacttcc gggtcgccct ccatcagttt cgtggccgtc cagcgcaccg
    22861 tataggggtg ctcgacatag gggtcgatcg cggcggcgat cttccgatag atctcttcat
    22921 gatccggatg gatcgcggga atgcggggat ggcccggttc cttcccttcg gccaatgccg
    22981 tgaggcgggc caccagttcc cggatacccc ggtttttcgt ggccaccatc gggaccacgg
    23041 gcacccccag cgcccgttcc agcgcctggg gatcgacctc gatgccttgg gcttccgcga
    23101 cgtccagcat gttcacggcg acgatgaccg gcgattccaa aagcaggatc tcggacagga
    23161 gatagaggcc gcgctccagg gcggcggcgc tgacgattag gatgatgacg tcggggtgga
    23221 cgtcgatgat gaaatcgcgg gccacccgct cttcctccga ggaggcggtc aggctgtagg
    23281 ttccggggag gtcgacgatc cgcatctcga acgcctccgt cgcgtgggtg ccctccttct
    23341 tttccacggt cttgcccggc cagttgccga cgtgctggga gagacccgtc aggatgttga
    23401 acagggtgga ttttcccacg ttgggctggc cggcgagcgc cacggtcaac ccccggctgg
    23461 cgcgccgggg cgcgggcgcc tccggcgcct cccgcagatt ggaggcgagg atttttgccg
    23521 cctcgccgcg gccaagggcc acgcgggtat cggacacctg gaggatcacc cagcccccgc
    23581 tgttgtgcgt cacacgcacg cgggcgccct gggcgacccc cattccggcg agccggctcg
    23641 tcaggccgcg gcccccgctg acggcgtgaa tcaccccctc ctccccttca cggaaggcgg
    23701 ttatggctat cctctcgtcc tgcatcccat cctcatcccg ggttgcccac gcccggcgcg
    23761 aacgacggca cggtttcccc gcggtgccgc cggtcccgtt cccacgcggc gaaacccttt
    23821 taacgtgatt caccggcctt gtcaaagtct tatccggacg ccgcccggga cggaagacat
    23881 ccgcgttgcg tgcgcccgtt tccccccggt cttcgtcatt gacaaagcgg tgattttggg
    23941 tgcaggatgg cccatcccat ccgcaagatc ggccgaaagc catgacccga cacctccacg
    24001 tcacctgcgc catcatcgaa cgcgacggac gggtcctggc ggcgcagcgc ggcggaggcg
    24061 ggagcatggc cttcaaatgg gagttccccg gcggcaagat ccggccgggg gagagcccgg
    24121 aggcctgcct gcatcgggaa ctgatggagg agatgggcct gcgcatcgac gtgaagcgcc
    24181 cgctcgaccc cgcaccgcac cgctacccgt tgttcgacgt gaccctgtac cccttcgtgt
    24241 gcacgatcgc cgcgggcgaa cccgtgctca atgcgcaccg ggccgtcgtc tggctggccg
    24301 cggaggagct gcccgccctg gactgggccg aggccgatcg gccggtgatc gccgcctacc
    24361 gcgagagcct ccgcgggggc gatccctctc ctccgatacc gaagtgaaac cgggggctca
    24421 tccgccgttc gggatgagcc ggcctcgtca actccatcca gaagcatctg acaacgattc
    24481 agggagaatg ccatgaaaaa aggacttggg atcgtctcgg tgttgatcgt cgtcgccctg
    24541 ggcgcctacc tttgcttcca ggtctttttc cgcatggccg tccccgggga tacggggacg
    24601 ctcgaaatcg acggactgcg gtcggcggtg gaggtgcgct tcgacgacta cggggttccc
    24661 cacctcttcg cgaaaaacga tgacgatctg ttcttcgccc aggggtatgt cgccgcccgc
    24721 gaacgcctgt tccagatgga gatctcgcgg ctggccggcc gcggcgaact ctcgtccctg
    24781 ttcggcgaga agaccctgga caaggaccgg ttcctcaaga cgatcggcat ccaccgcctg
    24841 gcccggaccg gatacgcggc cctgtcgccc gaggcccgcc ggctcacggc ggcctatgtg
    24901 gccggggtca acgcctgcat ccgggacacg aagcgcctcc cccgcgagtt catcctcctg
    24961 ggggcgaagc ccggacgctg ggagcccgag gactgcgtcg ccacggtcct gctgatgtcc
    25021 tacagcctca cgcggtcggt gtacgtcgat ctggtcctct accgcatcgg cgaacggagc
    25081 ggggagggac tcgtcaaggc gatcgccccg tcgtaccccg atttcgcgcc gaccctgacg
    25141 ggccggcggc tgagcccggt tccccgcggc cttctgaagg gcgtcttcca ccgcttcgcc
    25201 gccgcagacc ccggcgatat cgctcccggc cggcctgtgc aggagatgcc ggcgagcaac
    25261 tggatgatct tctccggcag gatgaccgag accggcaagg ccctgttcgc cggaagcccg
    25321 gatctgaaac ccaccctgcc cgccctcttc tacatcatgc atctgaaggg ggggagcttc
    25381 gatgtcgccg gcggggcgat ccccggcgcg ccggggatcg ggcccctggg ctacaacggc
    25441 cacatcgtct ggagcgccgt caacggcagg ggcgatgagc tcgactacta cgtcgaaaag
    25501 atcaaccccc aaaacccgga ccagtacctc acggaaacag gataccggac cttcatgacg
    25561 cgcgaggaaa ccctgcgcat caagaccaaa ggcggcctca agcaggagac gttcctggtg
    25621 aaattctccc ggcacgggcc gatcgtctcc ggcgtgatgc cgatggcgcc ggcccactgc
    25681 gccatgaaat gggccgcctt cgagaatccg gccaccgacc tggagggctt gctccacctg
    25741 aaccgggcca agaactttac ggcgtttcgc cgcgcactca aattcgtccg aaacaccaac
    25801 ctgggggtcg gctatgcgga ccgggaaggc aatatcggct ggcagttcac cgcctcggcg
    25861 ccggtccgca agaacgggga cggctcctat cccgtgccgg gctggaccgg cgcgtatgag
    25921 tggacggggt atctgcccta cgaaaggctc ccctacgatt acaacccggc ggccggatac
    25981 gtggcgtcgt tcaacaacga tccgggcaac gcgtccgacc acctgaccaa ctattatctc
    26041 ttcgaacggg ccacccggtt cggcaacatc atgaaggagc gcggaggccg caaggtcggc
    26101 atcgaggact tgagggccat gcagctcgat accgtgtccg tggtggcgca gcgctgggtg
    26161 ccgaagatcc ggtccgcctg cgccgacgag gcgtatgccc gatacaccgc cctcttcgag
    26221 ggctggaacg gcgccatcga catcggcagc cccgccgcca ccctgttcaa cgccttttac
    26281 gcccggttcc tcaaaaatac gctgggcgac gaggtcgggc cgaaactctg ggaggagggt
    26341 ctgagccaat cctacctctt ctacatcccc gacctcgtcc tggccaggat cgtggatgaa
    26401 cccggccaca ttctgtacga cgacacggcc acccgggacc ggcgggagac ccgcgacgac
    26461 atcatccgga aaagccttca ggaagccgtc gggcaattga cggaccggct cggcgacgat
    26521 ccgcggaaat ggcaatgggg gcgcgtccac aagatgtatt tcgatcaccc cctgggggca
    26581 aagctcgggt ttttcaacct gaagcccatt ccgacccacg gggagcatca cacgatcaac
    26641 tcgggcttct gggacctcaa ccagcccttc aggatggacg cgggcggcgt catccgtatc
    26701 atcgtggatt tcgcccgccc ggagaattcg acgatcatca gtccgccggg gcagtcgggg
    26761 gtttacaaga gccggcacta cgacgatctc gcgcagctct gggcggacgg cggccagatc
    26821 ccgctgcact tcttcacggc gagggatctc ccgaatgtcc tgaggctcca accgaaagga
    26881 cggtagaggg gcgcccgtca gggtcaccac gtcgtccgtt gtgctcacgc cgatcttggc
    26941 gtccttgagg gacggaacgg ccgccagctt caggttgact tccgtcgtga tcgtggcatc
    27001 atccacgaag gccgtcgcct tctgtttggc cacgccggcc gccttcccct ttgccccgac
    27061 cttgatatcc ccgaccgact gcgcataggc cgtcgacacc cttgccagac aaacgacgac
    27121 ggctgcgatc tactttttca tgtgctcctc ctttgaaaaa catttttccg ctgcttagtc
    27181 catcccggaa aatgcagcca acgggaatta ttgtcttgac gccccgccgt cttttgacta
    27241 tgttctcgcc cgcgatcgat gcatgcatta tatgtagcaa acctatagca atgttaatgg
    27301 tttaatgcat gacgacgagc gcgttggaga gatcactacc gtcaggaggt tttttcatga
    27361 gggatattgt caatcccatc gagattcgcc tcacccaggg gggcggaact cccgtcggcc
    27421 tgaaaaaggt cgcatcgccc gtcatgaaga cggacgcctg catcaactgc ggcacgtgcc
    27481 ttcgggcctg tcccaccggg gcgatccatg agctccagcg gcagatctgc cgcctctgtc
    27541 cggactgcgc cgacagcccc atcctgttcc cgcgggacat ggaggcgctg acggcggaat
    27601 cctgcgccgg cgcctgcccc atcggtcatt atccggaggg ctatgtcaac caggtcgcgg
    27661 agggcgcctt cgaaaaggcg tgggagcgga tcatcgccgt caaccccctc ccctccgtgc
    27721 tggggcggat ctgctcccgc ccctgcgagg acgcctgcaa gcggggcaag ctgatcgacg
    27781 cccccctgcc catccgggcc atgaagcggc tcgtcgccga catggcctac gaaaagggct
    27841 gggtcgaaaa ggggtgttac ccgcgcaagt acggcgagcg cgtggccatc gtcggcgccg
    27901 ggccggcggg cgtcgccgcc gcccacgacc tgtcgatgcg gggctatgaa tgcgtgattc
    27961 acgacgcgtc gccgtccttc ggcgggatgc tcgcgaagtc ggtgccgtcg ttccgtctgc
    28021 cggcccaggc cctcgaacgg gatttcaagg cgattctggc ccggggcgtg accttccggc
    28081 cgaacatcga gatcggccgg aacccctcca tcgccgatct gctcgcacgc gagtgcgacg
    28141 ccgtcctcgt ggccgcgggc gccccgaagg gggtcaagct ccccatcccc ggcgccgatt
    28201 tcatgggcgt tttcaacgcc gtcgatttca tgacctccgt caaggcgggc ttcccgctca
    28261 aggtcgggga gaaggcgctc gtcatcggcg gcggcagcgt cgccaccgac gtcgcccgga
    28321 cgctgctcag gctgggcgcc ttcgacgtgg ccgttgcctg cgtcgagggg gaatgcgaca
    28381 tgccggcctt cagctgggaa cttgaggagg ccgaggacga aggcgtccgc ttcatccgtt
    28441 ccgccgctcc ggtcgccatc ggcggggact ggcagaaggt ccagtgggtg gatatggacc
    28501 ccgtcacgcg gtttgccctg ggcgagtgtg ggatcgaatg cgacacggac agcacgggca
    28561 ggttccgcgt ggaggccgat acggtcgtct tcgccgtcgg ccagaagccg gaccccggcg
    28621 tcttcgcgaa aacggcgggc gtcgaaatgg atgcaagggg gcgggtccgg atcgacccgt
    28681 ccacccaaat gacgaccctc gcgggggtgt tcgtcgcggg cgacctcgtc gaggccaggg
    28741 gatccgtggt cgaggcggtg gcgtcggcgc gccgggccgc cacggccatc gacgcctacc
    28801 tgaggggccg gaccgctccg gcgcccaagg aaaaggcgcc ggtcggggcg ccgatcgacg
    28861 agaagatttt ccccgtgcgg ctggagaagc tgaacgttgc cggcattccg gccctgctgc
    28921 cggaggacgc cgtgacgggc ttcgacgaag tggagggcgc aatggacgtc gcccaggcgg
    28981 tcgaggatgc ccgtcgctgc atgaaatgcg gctacgtcga tgtccgtcat gacctgtgca
    29041 tcgggtgcgg cacctgtgcc gcggtctgcc cgcagggaga cgtcatccgc atggatcggc
    29101 cgcgcacgcc ggaaaacgcg gcgccggcca aggaggtgca gtcatgaaaa cctgggatgt
    29161 catcgttgtc ggagcggggc cgggcgggag cacgaccgcg gccaggctcg ccatggcggg
    29221 gtgcgacgtg cttctggtcg acaaggagcg gtttccccgg gaaaaaccct gttcggacat
    29281 ctacggacgc tacggcgtcg atgcctacaa ggagatcggc gcctacgacg aactgctgga
    29341 gaagggcttc ctctttccgg agctgatgct cacctctccg gactacacgg cgctcaaagg
    29401 cccgaacgtc ctgagcgtca cctgcccccg gcgcgtgggg gacaacatcc tgaaggaggc
    29461 cgccgcgcgg cagggcgcca aggtgctcga ggcgttctgg gtgaacgatc tgatgatgga
    29521 ccgcgggcgg gtggtcggcg tcaaggccaa gtacgaaggc aagttcgtcg aatacccggc
    29581 ccggatcgtc atcggcgcgg acggcagcca cagttgggtg gcgaagcacc tgggtctgtt
    29641 ttcggacaac tacgacgagg tgttcctggc cggccgggcc tactacgcgg actgcgatcc
    29701 gccgctgagg acgatggagg tgcactacga tccctatttc ttcccgggtt tcgtctgcct
    29761 gagcccccac ccgggctacg gcgacgtcgt caacgtaggc atcggctacc agatgacacc
    29821 gtacgttcag gccctggagg acgtcgaggc gctgacccgg aagttcatcg agacgagccc
    29881 cttcggagag aaactgcgca aggcccggaa ggtcagcgac tggatggggt ggcgcgtgcc
    29941 gagcgccggc cagatcggca agacctacgc ggccggcgcc atgctcatcg gcgacgccgg
    30001 cagcttcgtc gatcccttca tcctcgaggg catcgccaac agcgtccgga gcggcggctt
    30061 tgccgtgcag acggccctcg aagcgctcga aaagggcgac ttttccgagt cgttcctgtc
    30121 ggtctacgag gcgcgctggc gggagaagat gaagcctcgg ctcgacgcgc tccagcagat
    30181 ggccctggtc gcccggaacg cggagatgct caacgccacg ttccagagcc tgaaggcgaa
    30241 cccggcggcg ctcaagaaga tgtttggata acgttcatgc catccgttgc gtcgggggga
    30301 tccgggggga tcctcccggc cggcggccgg cctgagggtt gggggggcgc cgcgccggcg
    30361 cgggacagaa aggaggcatg gcatgggaat catgacgagc gaagcgtacc tggcgagctt
    30421 ggcgaagcag aaacccgtgg tctacatcgg gggcgagaag atccacgacg tcgtccacag
    30481 tccgtacttc aggatctccc tggaagagat gtgcaagttc tacgattggg cgaacgatga
    30541 acgaaccgcc gcggatttca cgttccggtc gcatctcatc gacgaccggg tgagcttctg
    30601 gacgcacctt cgcgaaaccc ccgaggacgt ccgcaggatg atcgaggtga tgaaggcgca
    30661 gaacgcccgc catttctgct ccatgtgcat gatcacgggg ctcaacgtcc tctgggcggt
    30721 gacgtacgac atcgatcggg cgaagggaac gcagtaccac gagcggctga aggcgttttt
    30781 ccgggaactc cagacgaacg atttccgcta ctgcatgggc gtcatggacc cgaaaggaga
    30841 ccggtctctc gccccctccg cgcaggcgga cccggacctc tatctgcggg tggtcgagcg
    30901 gaagacggac ggcatcgtcg tcaacggcgc taagctccac acgtccaacg cgcccgtcac
    30961 ccatttcatc ttcacctgcc cctcgggcgt tctggcggaa gcggaccggg actacgccgt
    31021 ctctttcgcc gtcccggtgg acaccccggg gatcacgtac atcacgcgac cggcgccggg
    31081 tcccccggcg cccaggacgc tggagagccc ggtgagcggc cacatcggat tcgtcgagtg
    31141 cctgaccgtc ttcgaccatg tcttcgttcc gtgggagcgc gtcttcatgt gcggggaatg
    31201 ggagttcacg ccgaacctga tccgatactt ctcgccgtac gtccggacgt gcaaggggac
    31261 ctgcacctcg gcccggacgg acatcctgat cggggccgcg gcgctcatct cccaatacaa
    31321 cgggacggcc cgggcgagcc acatccggag caagctgacg gacatgatga tcgcttcgga
    31381 gatcgggtgg gggtgcgccc tcgccgccgt cgcgaagtcg gtcatgcatc cgagcggcat
    31441 ccctatcccc gacacgtccg tcgcgaacgc gggcctctac catggccgcc tgcgcttcgt
    31501 cgagtttctc ggtgccctcc aggagatcgc cggcggcatc gtcacgacca tgccggccga
    31561 gacggagtgc ctgaacgagg cgtcgcgggc gtacatcgac aaatacctca agggtgtcga
    31621 cgacgtcccc accgaggatc gtctgaagct gctctatttc attcaggatc tgacggcttc
    31681 gcgcttcggt ggctacctga cggccagcgc cgtctgcgcg ggcggaacgc cggaaacgaa
    31741 ccggatcgag gtgttccgga attacgatct tccgcaaaag atccgcaacg tcaaggaact
    31801 gtgccgcctc gcctgacgtt cggcgggaga cgggatcagg tgccgtttcg agccggtgat
    31861 cacggcgtgg gcgggtcgac gtgggcggcg atccgctccc ggaccatcgc cgccgtctcc
    31921 tcgacgctca tcgcggcaaa ggcctcgcag gggatctcct ccaggatccg cacccggatc
    31981 ttcttgacac cgtggaactt gaagctgtat ttcggcagcg catcgcgcgt tccggaaatg
    32041 gcgacgggca ggatgggcag gcgattatcc cgggccaggc tgaacgcccc cgtcttgaac
    32101 ggtcggagga ggcccgtctc cgaacgggtc ccctcgggga acatgaagat cgagttcccc
    32161 gcggccagcg cccggttgca gtcctcgagc atctgccgga tgctgtcctt gtcgccgcgg
    32221 accagcagga tatagcggtt cagccgcatg ttccagccga tcaggggaat cttgaagacc
    32281 tccgccttcg acacccattt gaaggggaag aagatgcgga agacgaccag gatgtccagg
    32341 agcgactggt gattggagac ggcgacgtag gtctttcggc cgatacgctc ccggccctcg
    32401 atggagaccg accaggccgg catcacccag aggtagaagg aggcccagaa ggagctgtag
    32461 tagtggagga ggagcaggcg ccggtcgaag aggaccgtca agagccagat cgcaagcgcg
    32521 atcgcgaaga agaacaggct tgtgacggcg atgaaaacga gaaacaggat tccgatcacg
    32581 cggtccatca tggctcgatt cccccccccc tttgtccgga atgtaacgtc cctttctttt
    32641 ccccgaaacg gccgcttccc gaacggcggc gtcccgatcc cataccccgg atgggctggg
    32701 aatgtcaatc tgcggcagca cccccagcgg aaaatccatc ccccgggagt cgaccacggg
    32761 gagttgcgcc tccggcttgt gtctcttcgg gatgttcggc aggtgtccct cgacggttcc
    32821 cctgagccgg cgcacggtga acgtctcggt ccacccggcc ggtcgccgtc tcccagagtt
    32881 tgaggggctt gtcgtcgctg ccggaaacgg catgtgtccc gtcccgaatg atggcgatgg
    32941 cgttgaccga tcccgaaggc ctccgcccgt caccggccgc agcagcgttt gaacttgagg
    33001 ccgctcccgc aggggcaggg cgcgttccgg cctgtgcgcc cgcccgcggc ctcctgccgg
    33061 cgacgcgcat cttctgatgc ggtccggcgt cgccagtcgt cgaccagacg cccgaattcg
    33121 cgatccgcgt gagcgaagaa cattttgtat gcaccgcaga agtggctgac gccgagatcg
    33181 cgcgggtcgc gccgccggtc cttggggcac ccgccccggc agcgggtgag ccaggggcag
    33241 gcggtgcacg ccccgggcag cgaggccttg cggcggccga attcccgctg ccggtccgac
    33301 acgaacagac cggccagcgg ctcccgcatc aggttgccca gcctttccgc cggttccaca
    33361 aagaagtcgc acggatacac atcaccgttg tgctcgatga cgaggtagtc gccgcagtgc
    33421 tcgcacagag tgcagtccgg cggcgccatg cccgcatagc cgaacaggag cgactcgaag
    33481 aagcgcaccg aggtctgtgg gcggccgtcg cggaaatcgg cgcgccaccg gtcgaagatc
    33541 cggcagagaa acacgccgta agcctccgcg ttgacggaga agggcgcggc ccgccccggg
    33601 tcggacgggt ccgtctccac gcaggggata aactgcatgt aatcgaggcc gagcgcgcgg
    33661 tggtggtcgt agatctcctc cgggtagcgc acggtgacgt cgttgatcac cgtgagtgcg
    33721 ttgaccggca cccccgcgtc caggagcatc cgcgccgcgt cacggacccg tgaccacgtg
    33781 cctccgccgc cccggagggt gcgataccgg tcgtgcaggg actggggccc gtcgatcgag
    33841 agcccgatca ggaaggcgta cttccggaag aaccgcgccc agcggcggtc gagcagaagg
    33901 ccgttcgtct ggaggccgtt gccgatcgtc ttgccgccgc cgtacttctc ctcaagccgc
    33961 acggcccgtt cgtagaaggg aagccccatg agcgtgggct cgcccccctg ccagccgacg
    34021 gacagttcgg gcgcggggga ggccatgaac tggcggagcg tctcgtccag gacggcatcg
    34081 ctcatccggt gggttggggt tcccggatac aaccccgcct tgccggaata gaagcagtag
    34141 ccgcacgcca tgccgcagtc cggccccgcg ggcttgacga gaatggacct gagcgccgtc
    34201 gccgccatcc gctacgtctt cagccacctg cgcggcttcg ccgcgacctg ccgcgtgatg
    34261 tgccggtcag ccatggcaag tcctcctttc cgttcatgcc ctgaagggtc gggatcaaag
    34321 atgcgtcggg cgctcaaaaa agtcaagcgg ttgatcccgg cgcggtccgg cccgccataa
    34381 cggccgtttc ttccggtcgc tgctattcgt agtgcagcgc gtcgatgggg ttcaactggg
    34441 aggccttgta ggcggggtag aagccgaaaa acaggcccac gagaccggag aagccgaagg
    34501 ccagcaggac ggacgggacg gagatgacgg tggaccagcc ggccaggatt gacaggagct
    34561 tcgcgcccgt gacgcccagg acgatcccga tcacgccgcc gatgagcgac agggtcaggg
    34621 cctcgatgat gaactgcagc cggatgtccc aggtcttggc ccccacggcc atgcggatgc
    34681 cgatttcccg ggtccgctcc gtcacggaga cgagcatgat gttcatgatc ccgatgccgc
    34741 ccacgaggag cgagacggag gcgatcgccg ccaggagcag ggccatgatc cgggtggact
    34801 gctcggccgc ctgcatgatc tgggtcaggt tccgcacggt gaagtcgttc tcctcgcgcg
    34861 gcgtcaggcg gtggcgttcg cgcaggagtt ccgtgatctg cgcctccgcc gtcgccatct
    34921 cgtcggcgct gcgggccttc acgacgatgt gccggaccat ccccggcagg cgcgtgccga
    34981 agatcttccg ctgcgccgtc gtgatcggga cgatgaccgt gtcgtcctgg tcctggccca
    35041 tcatcgactg ccccttgggc gccaggacgc cgatgacgac gaacgggatc ttcttgatcc
    35101 ggatgatctg gcccaggggg tcggcgctgc cgaagaggtt gtccgccacg gtccggccca
    35161 ggagacagac cttggtcgcg ctccggacgt cctggtccgt gaaaggccgg ccggcggcga
    35221 gcgtccagtc ccggacgttc aggataccgg gactggagcc ctcgatcccg gtcgcccagt
    35281 tctggttgcc gtacacgacc tgggccgtgc cgttgtgcat cggcgccaca tcggcgacgg
    35341 ccgtgcattc ccgggcgatg gcttcggcgt cgcccagggt cagggtggat cgcgtcccgg
    35401 cgcccatccg cagacccccg gcggtgctgc tccccggaac gacgatgatc aggttgctgc
    35461 ccatgctggc gatctgctgc gagatgcgcc ggctggcgcc ggatcccacg gccagcatga
    35521 tgatcaccgc ggccacgccg atgatgatgc cgagcatggt gagtcccgaa cgcatcttgt
    35581 tggcgcgcag cgcgcggagg gagatgcgga acgtcgccgg aatgttgatc atctccgcct
    35641 cctggcctgc atcgtcattt tgcctccatt ttcgatccgg gtcatatcag ggtccgcctt
    35701 gcgtcaatgt ttcacgggct cgtcgctgat cacgcggccg tccaggaagc ggatgatccg
    35761 cccactgaag gcggcgatgt ccggttcgtg ggtcaccaac agaatcgtcc gccccgcgtc
    35821 ccggttgagc ttgacgaaca gctccatgat ctcgacggac gtcttcgtgt ccaggtttcc
    35881 cgtgggctcg tcggcgagga tcagcggggc ctggttgacg agggcgcggg cgatggcgac
    35941 gcgctgctgc tggcccccgg agagctggtt cgggtgatgg gtctcccgcc cctcgagccc
    36001 caacatcttc agggactcgc gggccagccg tcggcgctcc ttcggcccga ttccgttgta
    36061 gagcatcggc agctccacgt tctccagggc actggtgcgc ggcaggaggt tgaacccctg
    36121 gaagacgaag ccgatcttcc ggttgcggat ctccgccagg gtgtcgcggt cccgccgggc
    36181 gacatcgacg ccctccaggc gatagccccc gctcgtcggt tcgtcgagac agccgatgac
    36241 gttcatgaag gtggatttgc cggagcccga cgggcccatg atggccgcga attccccggg
    36301 ctgcacggtc accgagacct cgtccagcgc ccggatgctc gtctcgccca cggggtagat
    36361 cttcgacagc cggtctgttt cgatgagcgc catgacgcct cttcaccttc ctgcgggggc
    36421 tttcccggga tgcgccgtcc gggaaagacc gggacacatc gatggccgcc cgaccgcgat
    36481 ccgcgacgtc gggtccgtcc cgccggcgtt taacggaaga atcccgacgg ccggggaacg
    36541 ttcgcctgcg gcgatgcggc gcccgcgacg tcgaggatca cgacgtcgcc ttccttgagg
    36601 gggccggcgg tcagttcggt ccggttgccg tcgctgatcc cgatccccac gcggacgcgc
    36661 acgggtttgc ggttggacag gatccacaga tcccgcatga tttgtccggg aggcgtccga
    36721 gcggcgggac tcccggcgac cgcacccgtt tccctggccc cgaccgtcgt cttttccgcc
    36781 ggtcccttga agcgcagggc ggcgttcggg atcgtcagga tgccgttccg ggtcgccgtg
    36841 atgatggaga cgttggccgt cattcccggc ttcagtttga gttccggatt ggccaccttg
    36901 acgatgacgg tgtaggtgac cacgttcgat accgtcgtcg gggcgtttcg gacctccgaa
    36961 accttcccgg tgaagttgag atcgggatag gcgtccacgg tgaaccggac cgcctggccg
    37021 acccggatcc tgccgatgtc cgcctcgtcc acgctcgtgt cgatctgcat ctgcgtgaga
    37081 tcccgggcaa tgctgaagag ggtgggtgtc tggaaacttg ccgccacggt ctggccgacg
    37141 tccacgttgc gcgaaatgac ggtgccctcc accggcgaca ggatgcgggt atagcgcagg
    37201 ctggtctcct ccgcgttcag cgtcgcccgc gcctgggcga cttgggcccg ggcgacgctg
    37261 acctgcgcct gggccgaccg gtgggccgtc tccgccgcgt ccaggtcact gcgggcgatg
    37321 tagttccggg cgtagagcgt ccggttccgc tccagggttc gttgggcgtc ggtgagcgct
    37381 gactcagcct tctcgacgcc ggcgacggcg gactgcagac cggcgcgcgc ctggtccacc
    37441 ctggcctggg gcagggccgg atcgatctgg gccaggagct gacccttccg gacaagcgaa
    37501 ttgtagtcca cgaagatctg cttgatcgtg cccgacacct gcgtgcccac ggtgacggtg
    37561 gtgacggcgc tgaccgtgcc cgtcgccgtg acggccgcgc tcaggtctcc ccgggttacg
    37621 cggtcggtcc ggtagcggac agtctcgcct ttcttctgga agaggaggta tccgcccagc
    37681 gcgagcgccg cgaccagaac ccccgtgatc acaagtttct ttttcatcgt tctcccgtcg
    37741 ccttctccag gttcgcctgc gccacccggt aatcgtacag cgctgcggtg tgggccgttt
    37801 tcgcgttgct caccgccacc agggcgtccg tgacctcgat cgggctgccg acgcccgtcg
    37861 cgtaccgtcc ttgggccagc tccaggtttt cctccgcctg acggacggac agggccgccg
    37921 tatcgatccg gtccgacgcc tcccggagat tcagccaggc ctgcttgacc tccagggtga
    37981 cggcctgccg cagcgccgcc aggttggcgt cgagggcatc cagcgtcgcc cgcgcctcct
    38041 cgatctggta ctttgtggcg tagccgctga acagggggac gttcacctgg gcgccgacgc
    38101 tccagccgtc gtccagggga aagtcgcctc caccccaccc gtaactggcg tttcccgtca
    38161 ggaaggggta atagtccttt ttggcgaggt tgacggtctc ctcctgcgcc tggacccgga
    38221 cggtcagcgc ccggatttcc ggtcggcggt cataggccag gcggatggcc tcgtccagcg
    38281 caaccggtgc gcgcgtcacg gagagcgaat cgacgatgac gaactccggg gcctccggcg
    38341 gcaggccgat ggcgttgctc agcgacacct gggccagtct ccaggcgttt tcggccctga
    38401 ggagggtgag cttcgcgccg gaaagatcca cctccgcctt ggtgacgtcg aatttcggct
    38461 tcgtgccgac gtcgaagaac cctttggccc ggtccaggtg ctgctggaac tgacccaccg
    38521 tctcccgggc gaccccccgg tcccgttcgg atttcaggag ctcccagtag gcctgcttta
    38581 cgccgaggat gatctgcgcg cggacctgtt cgaaattcgc ctgggacgaa tcccgctcca
    38641 gttcacggat gcggacccgg gaagaggttt tgccgaagtc gtagaggttc tggctcaggc
    38701 tgagcctgga agaatagctg tcggaggggt tgctggatct ccccgcggcg cctcccgcgc
    38761 cggagggatc cgaccggctg tagcccgccg cgccgctcaa ctgcggatag tagtccgcgc
    38821 gggcttggcc gatgcgggat tcgcccgcgc ggacggtatg ggtggcggcc tggagatccg
    38881 gatgccgact gagggcgatg gcgaggcagc gttcgagggt cagggtctcc cccgggcgga
    38941 tcgtatcatc cgcgcccgcc gccgcgggga gaagggccgt catcaaaagg agaaaaacaa
    39001 tcgttttgcg catcggtacc atcaacgatc cgggcagggc gcgccccagg acttcggggt
    39061 cgcgcgtttc gttccggttt ctttttcctg gaaacgccga tttctgcaac gatggacagc
    39121 aatcggcatg ccatcgtgcc ggttccggcc gagcatgcgc aaggaaccgg catcattgag
    39181 aaatgaacga accggcattc cttccccggg gatgaggggc gggtgcctgg ataataaaaa
    39241 tccggacaga atctccggtt ggatcaaacc tatccacgcg gaggcctcgt ccggtctatc
    39301 aaacgtccgg agaaccccgc ccttgaacga gacctatgca tacccgcctt tgacggagaa
    39361 ggcaaaccaa aatgaatgaa aaagtcggga acggctctgg ctcacaggcc ttttgtatga
    39421 taaagtgcct ccctctgcag cgagggttgt aacttttttg tccgtatccg ggggcgtggc
    39481 gtgggactgc tcaaactgct catcgacgat ccgctgacat tcgtcctttt ggcgataccg
    39541 ctcctgtact ccatcatcgt ccacgagctg gcccacggtt gggtcgcctt gcgcatgggc
    39601 gatccgacgg ccaagaacgc gggccggctg accctgaacc ccctgagcca tctcgacccg
    39661 atcggcacga tcatgctctt catcgtcggg ttcggctggg cgcgcccggt gccggtcaac
    39721 ctgggctaca tccgcgatta ccgccgggga ctcgtgctcg tctcgtcggc cggcatcgtc
    39781 gccaacatga tcttcgcctt tctcgccgtc ctgctgctca agtggctcac gcccgcgccg
    39841 cagagctcgg tcggcatctt cctcttctac ctggcgcaga tcaatctcat tctcgccgcc
    39901 ttcaacctga tccccatccc gccgctcgac ggctcgaaga tcctcatggg cttcctgccg
    39961 gagcggatgc aggacgccct ggcgcgcctc gagccctacg gcttcttcat catcatcggt
    40021 cttctttttg tgggggccct gaacccgctc atccgcttct tccggtggct catcctcgtc
    40081 ccgatcgggt ttatcctgtc ctgagacgcg gatcgccatg gcccgtgcga ttccggagga
    40141 agcgggcatc ccgggcccat gcgggatgac gaccagtggc gttcggcagg atgaaaggtg
    40201 gcgtgccggc cggggctgcc cgcgcccgga atcgccgttc ccggaggccc gttcgattga
    40261 tttgcccgtg cagtccgggt tgcaacaggc ctgttcaggt cttggaggcg tcgaccgtga
    40321 agtcgtaccg caaggaactc tggtttcagc ttcccgcgag gcgcgggttc gtgaacatca
    40381 cacccgaggt ggccgcgtgc ctgaaggaga gcgggatcgt cgaaggcctc tgtctcgtca
    40441 acgcgatgca catcacggca tcggtcttca tcaacgacga cgagccgggg ctccaccagg
    40501 attacgagca gtggctggag gggctcgcgc cgcacgagcc ggtgagccgc tatcgccaca
    40561 accgcacggg cgaggacaac ggcgacgccc acctcaaaag gcaggtcatg ggccgtgagg
    40621 tcgttgtcgc cgtcacgaac ggacgcctgg atttcggccc gtgggagcag atgttctacg
    40681 gcgaattcga cggccgccgc cgcaagcggg tgctcgtcaa gatcatcggg gaatgatctc
    40741 cgccgaaccc gggccgctca gggtcacttc agccgcgcct ccgagagatc caggtggtcc
    40801 gtttcgattt ccaccagcgc cgcgaagatc tgtcgaagtc tctcgtgatt ggattcgtcg
    40861 ccggcctttt gatagaaggc gatggccctg gtctcccgct catgggattc gttgagattc
    40921 ttgacgttct ccgtatggca ggtttcgttt cccgccggcg tcttgggaag gcgcaggact
    40981 tttttccaga tggccgcatg ctcggcctcg attttcccca gggccttgaa caacagcttg
    41041 ccctccgcgt catccgtttt ttccgccgcg caggcgtaga acgtcgcgtt gctcacctcg
    41101 acctgaaggg cgcgttcggc attggccctg tctttttccg tgagaggcac ctcgaaggtg
    41161 acgacggcct cccgggcctc tttgatgaac gcctgatggg ccccgcagaa cggacagtgt
    41221 gtcggggggg tctcgccgat gtaagcatcg ccgcagattt cgcatctcca caatttgacc
    41281 atgtttcacc tccaaacgtg ttctggatga acgaggactg gcccttccgg gctccccgga
    41341 agcggccgtt taagcaattc cttttcagcg gcccttgcgg gtccaggtga tctggaattc
    41401 gacctcttcc gcgccgccct cgcgctcgtg ctcgatcgtg tacacggcgt ccgccggcac
    41461 gaggatgcgc tcccccgcga tctggatcgc gaatcgatcg cccttctcca gggcgtcggc
    41521 cagccggcgg agtttggcga cgaacgcggc cgtcggatag cccttttcga tgtcccggtc
    41581 tttttttctt ttcatccgtc ttttcctttc cgttgcatga gaacttttcc cgcgcgggcc
    41641 gtgccctagg cgtttcccgg caggatcaaa tcctccagcc ggtccgacca ggcggtcagg
    41701 gggacgcacc cgtcctccgt cacga
//
LOCUS       DAOOLJ010000016        41414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_698_length_41414_cov_16.152905, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000016 DAOOLJ010000000
VERSION     DAOOLJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 41414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41414
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_41414_cov_16.152905"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PLW16_04720"
     CDS             <1..1438
                     /locus_tag="PLW16_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPB70243.1"
                     /translation="RKILIANRGEIALRILRTIKELGKGAVVVYEKPDSEAYYIRLAD
                     TAILIGEGPRCDYLNIERIIWAARKSGADAVHPGYGFLAENADFSEACEKAGLIFIGP
                     PAGVIRDLGNKVVARRIAAQAGIDTIPGTGDLPRGEDGVAAALKFGLEHGYPLMLKAS
                     SGGGGRGIRKVVNEKDLLVQLPMARQETLSAFNDENIYVEKCVEAPRHVEIQILADRH
                     GQIIHLGSRDCSIQRRHQKLLEIAPANLPVDVLEAMYAAAIKAARQAGYINAGTVEFL
                     VDARTNAFWFMEMNTRLQVEHTVTEELTGVDIVREQIRVAEGQVLDIPRERVHLMGKA
                     VQVRVNAEDPKNHFMPEGGKQVEVYQSPGGPGVRLDGVVYQGYRVPTEYDSLMVKMTI
                     RGYNWEQTIQRLKQALNGFLIVGPATTVPFYLAICDEPDFLAERFDTGYLDAHPAVFQ
                     YAEQEREVAKLARLIAEIHARKINPFAY"
     gene            1452..3392
                     /locus_tag="PLW16_04725"
     CDS             1452..3392
                     /locus_tag="PLW16_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HPB70244.1"
                     /translation="MTRRTQNNDARITPQELRRNGVRQVLEKIRRAGGYYVTNTERDL
                     SQSDFKNRIMPHTQFLVAPQRNDAGFFSIEITGGASVHVDMLRKQMNPLEKLEVLSAN
                     MPDTLFQTLCRGVNLFGYRPYPENVIRLTVRSFARYVHVWRVFDFLNHVSNMIPVFEE
                     VRESGAILEPSICFSTGPEHTDAYYVGKVGEILAVTGEDILLCIKNHGGLGTPKRIGA
                     LVRAIRERYPDLVIHYHGHNTDGADLGRIVAAVKNGAKIVDASDHAMTGFYGPPPLLT
                     AVDILSDEGYEAAGLNRQAVIDASNILRGEREHYRDFESQFLGFDPTAQIHKLPGGAT
                     GSSFEQAVKGGFLHQMPNILQNELPRVHVELGNWWSVTPGSQILWTTAVNNVLKGARY
                     RDANDDLKSLMLGRYGEFPFYRPSDSIYEAVFGKNWKAILEHEGGYQRIEDVDLAVER
                     KVLEHRIGRAATDDELVLYLQHPNDAVDFFKFEAKYGKTWVLSPNIWFRKGGFGLGDK
                     FEIPDAYGRLHAVEIGPKRRTKAGDAVTYLVIDHHPEPILTELEGEGVPAKKKISFTP
                     KEIEALAKTGDVRSQIIGTVSEIPISEGEEVSAGQILIVLEAMKMLNNVIAEINGRVA
                     EILVAPGDRVEVGDPLIVVRTE"
     gene            3477..5999
                     /gene="secA"
                     /locus_tag="PLW16_04730"
     CDS             3477..5999
                     /gene="secA"
                     /locus_tag="PLW16_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPB70245.1"
                     /translation="MIDAILRKIVGSKNDRELKRLGFLLEEINDLEPVFVALSDAELA
                     AKTPCFKEKIDNGASCDDILTEAFAAAREAARRTLGMRPFDVQIIGGIVLHEGKIAEM
                     KTGEGKTLAATLPLYLNALTGRGCHVVTVNDYLARRDAEWMGPVYLMMGLSVGVVVHG
                     MDDTERRAAYHADITYGTNNEFGFDYLRDNMKFTLDDYVQRDFHYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYYRINQVIPRLKKDQDYTIDEKSRTVALTEDGVARTENALK
                     VQNLYEPRNMEILHHVNQALKAHTLFKRDVDYVVKEGQVIIVDEFTGRIMPGRRYSDG
                     LHQALEAKEHVKVERENQTLASITFQNYFRMYEKLAGMTGTADTEAAEFKKIYNLDVL
                     IIPTNKPMIRQDHTDVIYKTEDEKFAAVIDEIRELHREGRPVLVGTISIAKSELLSKY
                     LTRAGIKHHVLNAKNHEREAEIIAQAGQRSAVTISTNMAGRGTDIKLGEGVADLGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDMGSSRFYLSLEDDLLRIFGAERISSIMNRVGME
                     EGEPIEHRLISRAIENAQKRVEGQNFDIRKHLLDYDDVMNRQRQVIYEQRKKVLRGEE
                     LWKDIESMLAEVIEDTLAQYIDEKHHHETWDLKGLQDWVWRQFGLKLDLAAGAGDPFG
                     AGTIGETIAAAVMGLLQAKQREFGKPLMEYLMRMIMLHAIDAQWKDHLLGMDHLKEGI
                     GLRGYGQKDPAREYQKEGYDMFMDMVWRIKEDTLQKLCMVQIRREEEVEEMRERQRQD
                     YIMSRGEDTPASQTVRREEKKIGRNDPCPCGSGKKYKKCCGR"
     gene            6028..7224
                     /gene="argJ"
                     /locus_tag="PLW16_04735"
     CDS             6028..7224
                     /gene="argJ"
                     /locus_tag="PLW16_04735"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPB70246.1"
                     /translation="MERNQTYIVPGFTASGIAAGIKADGRTDLALIRSDVPAVAAGVF
                     TTNRFPAAPVQIDRERIARGAARAILANSGNANAATGSDGLKDALASSRELARRLAIP
                     EHQILVASTGVIGHRLPLEKILAGLPRLVADLSPEGIPAAEEAILTTDRFPKLAVART
                     RCGGRDVTVCGLAKGAGMIEPHMATMLAFILTDAVVLRDDLDRLFRRAVRSTFNAISV
                     DGCESTNDTALILANGVAANRPIAARGADGRRFGAMLTEVMGQLARDMVEDGEGATKR
                     VEICVSGARTRAEARRIAYAVARSNLVKTAFYGGDPNWGRIIAAAGSVGIALTADRVT
                     LHFEDVLMFADGQGRAPDPERIFAIMQQPIIRIRLEVAMGKAAFVLQTSDLTRDYVAL
                     NAHYHT"
     gene            7375..8226
                     /gene="rpsB"
                     /locus_tag="PLW16_04740"
     CDS             7375..8226
                     /gene="rpsB"
                     /locus_tag="PLW16_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPB70247.1"
                     /translation="MAIISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVEMFQVAYNFVVKSVAEGGEILFIGTKKQSQEAIREEAERCGMPYVNQRWLGGMLT
                     NFVTIKKSIDRLNALDAMFADDSIKAFPKREILMRQKDKDKLDKVLGGIRRIKGRPAG
                     VFIVDPKREDIAVQEANKLGIPIVAIVDTNCNPDVIDYVIPGNDDAIRAIKLFSSRVA
                     DAVLEGKRRFEERIQAETDKASERTAAVPDEEGENDVPESVEAVGDAAVAVQPTGDKN
                     ATHEEVA"
     gene            8228..8830
                     /gene="tsf"
                     /locus_tag="PLW16_04745"
     CDS             8228..8830
                     /gene="tsf"
                     /locus_tag="PLW16_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPB70248.1"
                     /translation="MDITSAMVKDLRTKTGAGMMDCKEALTASAGDVEKAIEYLRKKG
                     MSAATKRSSKAAKEGVVSSYIHMGGRIGVMVEVNCETDFVAKTEDFQAMSKDIAMHVA
                     ASNPLFLKPEEIPPEVMEREKEIYRSQAMAEKKPEKIWERIIEGKLKKYYEEVCLLQQ
                     KFIKNTDITVETLVNNMIAKTGENIVVRRFARFQLGEDLK"
     gene            8866..9588
                     /gene="pyrH"
                     /locus_tag="PLW16_04750"
     CDS             8866..9588
                     /gene="pyrH"
                     /locus_tag="PLW16_04750"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPB70249.1"
                     /translation="MPKGKAVYRRALLKLSGEALLGAQAFGIDPEIVALVAGEIRSAV
                     ALGVQLGVVIGGGNIFRGGGTGARGMDRTTADYMGMLATIINSLALQGALEKQGVPTR
                     VLTAIEMRAVAEPFIQRRAIRHMEKGRVVIFGGGTGNPFFTTDTAAALRAVEIQAEVM
                     MKATKVDGVYDHDPNHNPSAVMYKMITYNEVLAKDLKVMDATAISLCRENRLPILVFN
                     LLKAGGIRRAVCGEKIGTIVQE"
     gene            9592..10149
                     /gene="frr"
                     /locus_tag="PLW16_04755"
     CDS             9592..10149
                     /gene="frr"
                     /locus_tag="PLW16_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPB70250.1"
                     /translation="MKALVYDELKENMEKALQSLERSFAKVRTGRASLGILDGIRVDY
                     YGQPTPLNQVATLSVPESRLIVIAPWDNSAIGAIEKALQKADLGLMPTNDGKVIRLSI
                     PTLTEERRRELVRNVKKMAEECKVKLRNARRDGNEQFKTLKKDNEISEDEMYTCQEEV
                     QKRTDKAIERTDALLAAKEKEIMEI"
     gene            10275..11012
                     /locus_tag="PLW16_04760"
     CDS             10275..11012
                     /locus_tag="PLW16_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPB70251.1"
                     /translation="MVSLDRAHLPRHIAIIMDGNGRWAQRHALGRITGHKRGARAVRV
                     AVETCRELGIGCLTLYAFSQENWQRPPEEVQALMHLLEDYLRTEIGRMIENDIRLVTI
                     GETQKLTAPVRAMLSNGMRKTEKNRSMILNLALSYGGRDEIVAAAKRAIREAEAGRLD
                     PDALTPERFSRFLFTADLPDPDLLIRTSGEYRISNFLLWQTAYTEFYFTDVLWPDFRR
                     KNLLEAIADYQRRERRFGLTSEQLSRE"
     gene            11188..12000
                     /locus_tag="PLW16_04765"
     CDS             11188..12000
                     /locus_tag="PLW16_04765"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPB70252.1"
                     /translation="MGMEQHLQRWITGIVAVPVLFAVIFFAPVPAFAAVIALFALAGL
                     YEFLAMTLPRATARQRGTALAAGALILAAATVGDAALILGVITLSVICVFLLHLLTIR
                     SEAFDLDPVYRIGFGLLYVPVLMAHFILLRQSPRGIAWIFFVIVLAFAGDIAAYYVGR
                     TWGHRKLQAVISPGKSEEGILGLVLGSIAGCLLYRTFFFPTLGWEHAAVIAGVGAVIG
                     QLGDLCESTIKRASGVKDSGFLLPGHGGVLDRLDCLLFITPFVYYYQLFVLK"
     gene            11997..13163
                     /locus_tag="PLW16_04770"
     CDS             11997..13163
                     /locus_tag="PLW16_04770"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPB70253.1"
                     /translation="MKTIAILGSTGSIGRSTLRIATEHSGRFSVGALAAGRNWRLLAE
                     QIREFRPHLAAVLDDEVARDLKVALGRGTPTDIVTGERGYGQVAELSDCQIVVSAMVG
                     AAGLRPTIAAIAAGKTLALANKESLVIGGALVTRLAREKGVRILPVDSEHSAIFQCLE
                     AHAVGVRRILLTASGGPFRSRPAGELARVTPEEALRHPNWTMGAKISVDSATMMNKGL
                     EMIEAKWLFDCDLERIDVLIHPQSLVHSLVEYRDGSVMAQLAVPDMRIPIAYALAYPE
                     RLETTAGRLDLSAAPPLEFFSPDEERFPCLALGRQAGRTGGTMPAVLNAANEVAVDAF
                     LRKRVGFMDIPRLIAAVMAKHRPADGSGLDEILEADAWARQAAADWIEEANPGQ"
     gene            13203..14309
                     /gene="rseP"
                     /locus_tag="PLW16_04775"
     CDS             13203..14309
                     /gene="rseP"
                     /locus_tag="PLW16_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPB70254.1"
                     /translation="MLGISIISLIILLGILIFVHEFGHFIVAKSFGVGVLKFSLGFGH
                     KLVGKKIGETNYLISWIPLGGYVKLLGDAEGETLSPEDEKRSFLNQTVWKRMGIVLAG
                     PVFNLLLALVVFFGVYLSGVPALTARIGDIQTESAAAAAGLRAGDTLISIAGQRVTYW
                     DDIARAVETGKGTPLALRIDRGGTVFDIQVTPRRVKGKNLFGEEVSAYKIGISPSGQV
                     VTKRLGPVNALAASVSQTWAVTKLTVISIVKLIDGTLSAKTLGGPILIAQIAGVQAKE
                     GVVPFLLFMALLSINLAVLNLLPIPVLDGGHLLFYLIELVFRRPVSLKWRERAQQVGF
                     ALLIMLMIWVFMMDVDRLHIKLLDDFARIFTKQP"
     gene            14306..15022
                     /gene="tsaB"
                     /locus_tag="PLW16_04780"
     CDS             14306..15022
                     /gene="tsaB"
                     /locus_tag="PLW16_04780"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPB70255.1"
                     /translation="MRTLAIDTAGISAGVSLAVDGQIRYEAFHNTGLNHSLVLLPAIE
                     AACRHAGVALAEIDLFALTIGPGSFTGLRIAAGTLKGLALAVGKPVVGVSTLDSLAYP
                     LTTSPDEVLCAMLDAQREQVYAAAYRNGVDGRRERIVPERVADLDELVASLPNGPILF
                     VGDGAERHRDMLAARSSALVRFAGPLYRHVRAAAVALLGEERYRAGESLDVVRFSPRY
                     LRVSEAERRHSSPRESTKND"
     gene            15097..15741
                     /locus_tag="PLW16_04785"
     CDS             15097..15741
                     /locus_tag="PLW16_04785"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPB70256.1"
                     /translation="MGKRNGIIVREGLPFILPLVLLTALAVLMGGGASVFVLGAVTVF
                     VIAFFRNPEREAPADRKLVISPADGRVIRIETVDAQELIPGTLKKVSIFMNVFNVHVN
                     RVPYDGTVSAIRYRPGKFLSANLDKASEQNERTAVLVRTWDDREILTVQIAGLIARRI
                     VCWLKEGMAVRAGERFGLIRFGSRLEVFLPDDATVLVQTGDKVRAGQTPIGRMP"
     gene            15771..16523
                     /gene="pssA"
                     /locus_tag="PLW16_04790"
     CDS             15771..16523
                     /gene="pssA"
                     /locus_tag="PLW16_04790"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPB70257.1"
                     /translation="MKKGIYVLPNLFTTASLFCGFYSAIASMKGLFEVAAIAIILAAV
                     LDALDGRIARMTNTTSKFGAEYDSLSDLVAFGVAPSLLAYNWSLAAYGKWGWMAAFLF
                     TVCGALRLARFNIQTGIIESRVFNGLPIPGAAAVVASGVLIFYKLGQEGRFESPLVLI
                     GVVAIAIFMVSNIKYYSFKDLNFFARKPFMSFVLIVFVLAVVIAEPQIMIFTFALGYS
                     LSGPAWWLFRLTRRAYEGFRQRRTEATKTDCK"
     gene            16592..17608
                     /locus_tag="PLW16_04795"
     CDS             16592..17608
                     /locus_tag="PLW16_04795"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPB70258.1"
                     /translation="MKQYNCAVVGATGAVGNEMIKVLEQRRFPAAELRLLASERSLGK
                     SLSYEGREIPVLVLTEESFAGIDIGLFSAGGAISERFAPAAVRAGCVVIDNTNAFRME
                     PDVPLVVPEVNPEAIGRYKDRGIIANPNCSTIQMVVALKPIHDAVRIKRIVVSTYQAV
                     SGTGKKAIEELAAQTRALISGREAEVNVYPHRIAFNCLPQIDVFLDNGYTREEMKMVR
                     ETRKIFNDPEIAVTATTVRVPVFYGHSESVNIETEQKITAAECRELLSRAPGIRVVDD
                     PGQRCYPMAIDAAGRDETLVGRIREDESIANGINLWVVSDNLRKGAALNAVQIAEVLI
                     RDYL"
     gene            17605..18156
                     /gene="rsmD"
                     /locus_tag="PLW16_04800"
     CDS             17605..18156
                     /gene="rsmD"
                     /locus_tag="PLW16_04800"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPB70259.1"
                     /translation="MRIIGGTAGKRRILVPPRGRLRPTEGRVKEALFQILPDMIGKDF
                     LDLFAGAGSVGLEAISRGAKRVVFVEKDRHLAAVIRKNLELLGFAAAGTVWDRDWASA
                     LRLLEVRGDRFDVVFADPPYERAFADRVLRRMATIGVLGEGGCFILQHSRREDLPDPG
                     EGWLHQVDRRMYGDTILSLFSAP"
     gene            18178..18669
                     /gene="coaD"
                     /locus_tag="PLW16_04805"
     CDS             18178..18669
                     /gene="coaD"
                     /locus_tag="PLW16_04805"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPB70260.1"
                     /translation="MKKIAVYPGSFDPITNGHIDIIKRGLRMFDEMIVLIAYNPNKKS
                     LFSVEERTDLIKEVLKCNGRVRIDSFSGLLVDYVKGAGANVILRGLRALSDFEYEFQM
                     ALINRRLNRDIETVFLMTGYKWFYTSSNIIKEAASLGGSVKGLVPDIVYQKLLDKYGN
                     QPR"
     gene            18666..19859
                     /locus_tag="PLW16_04810"
     CDS             18666..19859
                     /locus_tag="PLW16_04810"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB70261.1"
                     /translation="MNFARRIQRIKPSPTLAITMKANALRSQGRDIIGFGAGEPDFDT
                     PEHIRRAAVEAIEQGFTRYTPVGGIDELKDAVTAKLLRDQGLAYQRSQIVVSCGAKHT
                     LYNLAQVLFEEGDDVIIPAPYWVSYPDIVLLAGANPVILPTREEDGYKVQPSDLVRAF
                     TPATKAVILNSPSNPTGAAYSEAELRALGDVVRDRDIWVISDDIYEKIVYDGFRFHHM
                     AALDERLRAHTLVVNGVSKAYAMTGWRIGYAAGPADVIAAVTKIQSQNTSNTTSISQK
                     AAVAALNGDQGVVGAMVEEFQRRRDVIVAGLNAMEGVRCHTPQGAFYVFPNVARLFGK
                     SYDGRTLTGSADVAAWLLDAANCAVVPGIAFGEDTCIRLSYATSLANIETGLERIAAA
                     IKQLS"
     gene            19872..21269
                     /locus_tag="PLW16_04815"
     CDS             19872..21269
                     /locus_tag="PLW16_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPB70262.1"
                     /translation="MGGLFGVVKTVNCSKTLFYGTDYHSHLGTENGGLAVCGAKGFYH
                     TIHSISQAQFKSRFVDDYKEMDGTLGIGSIDDDAPQPLIIRSKFGTFAIAATGQVSNK
                     KALMAGLLDQCDSFNEMTLGGINTVELVAKLISRGSDIADGIERMQEAIEGSICVLVM
                     TADGIYAARDRYGRFPLTIGERIGGRGMAVATEASAFPNLGYQLCHFLGPGEIVFLSA
                     DGLKVLRPPRERRQVCAFLWIYTGYPASIYDGIGVEMARERCGRAMARNDDMEADLVT
                     GIPDSGVGHAIGYAMESGIPFRRPLVKYTPGYGRSYTPPSQDIRDLVATMKISAVREV
                     IQGSRMIVCDDSIVRGTQLKNFTIAKLWENGAREIHVRPACPPLMYPCIFGSSTRTFA
                     ELACRRAIRAIEGRDTDNVEAYLDRRSEEYRSMVEWIRRDLNVTSLKYLPIEDMIAAI
                     GLPETELCLYCWRGR"
     gene            21467..22531
                     /gene="ispG"
                     /locus_tag="PLW16_04820"
                     /gene_synonym="gcpE"
     CDS             21467..22531
                     /gene="ispG"
                     /locus_tag="PLW16_04820"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPB70263.1"
                     /translation="MRRKHTQSVFIGGVQVGGGAPVVVQSMTNTDTRDVRKTVRQVHR
                     LARAGCEIVRVAVPDAEAAQALRAIRKASRLPLIADIHFQYGLALAAINEGVDAIRIN
                     PGNLARDKIRRIAKAAGEAGIPIRIGINAGSLEKDLLAQYGGPNAPALVESALRHIDV
                     LRQSGFEAIKVSLKSSDVLTTLAAYREMSARSDCPLHLGVTEAGTEVQAAVKSSLGIG
                     MLLAEGIGDTIRVSVTGDPVREMPVAYGILRALGIRRRGVEIISCPTCGRCEIDLVTL
                     TKRMEKALAGCTTPLKVALMGCVVNGPGEAAEADVGIAGGRGFGFLFRRGKVIRKVPE
                     VEFVTALLTEIDALAERGDA"
     gene            22546..24273
                     /locus_tag="PLW16_04825"
     CDS             22546..24273
                     /locus_tag="PLW16_04825"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPB70264.1"
                     /translation="MRYSELFLPTGREVPSDAELASHQLMIRAGMIRKLTSGIYSYLP
                     MGYRVIRKVEQIVREEMNRAGAQELFMPMVQPAELWQESGRWVHYGRELLRFRDRHER
                     EYCLGPTHEEVITDLVRNEIKTYRQLPRNLYQIQTKFRDEIRPRFGVMRCREFAMKDA
                     YSFDANEAGAEESYRKMFEAYGRIFRRCGLDFRAVEADSGSIGGSYSHEFMVMADSGE
                     DAVVFCTACDYAANLEKAEIAPPEKTPSVDEARLPLEEVHTPGVRTIEEVCAFLSVTP
                     QEVVKTLIFNADGKPAAILVRGDHDVNEIKVKNVLGCDELELAMDDMILDVTGSPRGF
                     AGAVGIRARVIADYSLINMTHCVMGANREDYHLKNASPERDFSVSAYADLRVVRAGDP
                     CPRCAAPVRMARGIEVGHVFKLGTKYSRSMRAVYLDAAGKEQTMVMGCYGIGIGRTVA
                     AAIEQSHDDQGIVWPMTLAPYAVIITPVNSNEETLARASESLYQALSARGVEVLLDDR
                     DERAGVKFKDADLIGIPLRITVGPKKLAEGRVELRIRRTGEVRMLPLGEAVDGVSDLI
                     RAELERIGG"
     gene            24300..26450
                     /locus_tag="PLW16_04830"
     CDS             24300..26450
                     /locus_tag="PLW16_04830"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPB70265.1"
                     /translation="MLRITDIIDKAQAYLAPAELEMIEKAYVFSASVHQGQVRLSGEP
                     YLTHPMEVAGMLVDMRMDASTLITGLLHDTVEDTLATPEQIEENFGEEVAFLVSGLTK
                     ISKITFGSQEERQAENFRKMMLAMSTDIRILVVRLADRIHNMRTLQFQTPEKQRYIAK
                     ETLDLYAPLANRLGINWMKVELEDLSFAYLQPMAYEEIKRRIAMKKDERNQYTEKVRN
                     VLYQEMERFGLKGVVEGRAKHFYSIYKKMEEQHIDFDEVYDLIAFRMILDANREKECY
                     EALSVVHALWKPVPGRFKDYIAMPKANNYRSLHTTVIGPYGERVEVQIRTREMHEWAE
                     EGIAAHWRYKEGRSRGGKDDDEQVRKLRELLEVQQELKDPREFMSNLKLALFPDEVYV
                     FTPGGDVRSFPKGATPIDFAYSVHTDVGHQCIGAKVNRSIVPLKYQLQNGDTVEIITQ
                     SGHHPSKDWLKYTVTSRAISKIRAWIKTEERKKSVTLGRDLLEKEFRKHHLKFSQIAK
                     SDELKKIYEEHSLNSLEDLLAIVGYGRISPRHVVNLFLPEEDLKEEAVPEKIRRKATS
                     HEDFGISLTGVEDVMVRFAKCCNPIPGDEIIGYISRGRGITVHTANCPNFRDMDTERL
                     VEVKWSIREKHTYPVSLRVVCQDKKGILAEISSVIAGMDVNISQAEVETHSTEMQAVC
                     NFRMDVNDLQQFNQVVAALKKITEVLSTERVRGV"
     gene            26548..27243
                     /locus_tag="PLW16_04835"
     CDS             26548..27243
                     /locus_tag="PLW16_04835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPB70266.1"
                     /translation="MRHPLGKIRFCLVAAALLLLVAVAAWRPCWAAPLVLIDPGHGGN
                     ERGVRLSDAVYEKDVTFAIARMVRQDLIESGRIAVQLTRTEDRRISIQERADLAARLN
                     PRLLISLHVNAGFGNKSNGYELIFPGFGTTAPDGRAPQAAARFTAKHAYLNEGVRFAQ
                     VAARHLERVFPRKGRGLREAPIPLLEGLTVPAVVVEIGFATHPEERKRLLDPAIQKAI
                     ARALSVALSEYFR"
     gene            27240..27848
                     /locus_tag="PLW16_04840"
     CDS             27240..27848
                     /locus_tag="PLW16_04840"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF011397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HPB70267.1"
                     /translation="MKIVLASRNRGKLREIRDLLDGLGIEFVSLADFDHPPEVIEDGE
                     TFYDNALKKARTIAEYANCTALADDSGLEVEALRGAPGVHSARYAGAGADDLSNMEKL
                     LRDLEGVPPEKRTASFRCVVVLCHPDGCHQAFEGRWDGRISEAMRGRRGFGYDPVFYL
                     DEAGKTVAEMSPEDKNRRSHRAQAIRKLKRHLEDVRGAHGIK"
     gene            27849..27925
                     /locus_tag="PLW16_04845"
     tRNA            27849..27925
                     /locus_tag="PLW16_04845"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27883..27885,aa:Pro,seq:tgg)
     gene            complement(28169..29536)
                     /locus_tag="PLW16_04850"
     CDS             complement(28169..29536)
                     /locus_tag="PLW16_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPB70268.1"
                     /translation="MGMSPTINPEVFREYDVRGLVGRDLNPDFIYGLGRAIATLARER
                     GAKVMTAGRDCRLSSAEYQAAVMRGLIDGGMTVLDVGLCATPMLYFSIRHLGTDGGVM
                     VTGSHNPPDFNGFKICVGPDTIFGSDIQRLRTLMENGRYATGAGRAETADIRQPYEDH
                     LYGNVEITSGLSVVVDGGNGVGGLFALPLLRRFGCRIEDIYCDPDGRFPNHFPDPTVL
                     ENLQELIARVNASGADAGIAYDGDADRLGVITDQGKILWGDELLLLFSRFILEKNPGA
                     AIIGEVKCSQKLYDDIERHGGRAIMWKAGHSLIKEKMKEENALLAGEMSGHLFFADRY
                     FGFDDAIYASLRLLEILSKTGRRLSDLLADVPKTFVTPEIRIDCPDDRKFQIVEAVKE
                     RFRKDYPIIDVDGVRIPFADGWGLIRSSNTQPVLVLRFEAETEARLAYIRNLIEGTLQ
                     EIMSA"
     gene            complement(29552..30592)
                     /locus_tag="PLW16_04855"
     CDS             complement(29552..30592)
                     /locus_tag="PLW16_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB70269.1"
                     /translation="MNKLVVASMRQGAGKTSVIIGMAKALGKKIGYMKPFGERLLYRK
                     KRLWDYDAALITSLFKLEENPEDMSIGFHHAKLLYMLDEQATREKLGELLTNIGENKD
                     LVFIEGGRDLSYGVSVHLDPLSVAEALDADWLVVVSGDGDLVLDDVTFLKKRLGLTAG
                     RFRGVLINNVPNIDDFRDTHLPKLAQLEIPILGVIPYEKRLTYRTVGYLADRLFAKVL
                     SAEANLNRVARDIFIGSTSCEVAVRNPLFHEPGTLVLTTGDRSDMLLAALDAHAAAVL
                     LTNNILPAPHLISRAEELGIPLLFVTSDTYDAARQIDGIETLATRDDQEKIALIEEVF
                     RKHVDLAALDFA"
     gene            complement(30608..32728)
                     /locus_tag="PLW16_04860"
     CDS             complement(30608..32728)
                     /locus_tag="PLW16_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB70270.1"
                     /translation="MSDIHDDCIRYLFEPRSVAVIGASHERAKIGSAVLANIVSGGYT
                     GRILPVNPAGGVIEGRPVYPRVADIEGEVDLAIIAIPAKFVFDAVCQCADRGVKYGII
                     ITSGFSEIGNRQEENRIIDYANGHGMRIVGPNVFGVYSSAVNLNATFVDGNISSGHLA
                     MITQSGALGLTMIGQAEVENIGMSAIVSVGNKADIDEADLLQYLARHNDTRVILIYIE
                     GIRNGEKLVRILKQAARLKPVVIIKSGRSSRGAMAAASHTGSLAGADDVFDAIIRQCG
                     ALRAESTSEAFAWSKFLADSPLPAGENAVIITNGGGAGVMATDAFEKFGLGMLDDAQL
                     LKDVFTSATPSFGSTRNPIDITGQATAQDYTRAFDAALKCPEIHSVLGVYCESALFSS
                     DNLKEAIEDNYRRFKAAGKPIIFSLFGGEKTSTYVASARRRGIPVSDDVYPAAASLGA
                     MYAYHRYLHEPPEAIADATIDVEAIDRIVADVRREGRYFLLSHEARTIMTLAGIAIPN
                     SLTAQNLDGAIRSADQIGYPVVMKIVSRDIIHKSDAGGIALDLDNADEVMDAYGAIMR
                     NCRTSVPHAVLEGVEISEMVRPGTEMIVGARIDRAFGPILMVGLGGIYVEVMKDVSFR
                     SLPLERKEILGMIKSIRSYPLLLGVRGEETKDIDSLVDTMIKLGAIIQKCRGVSDIEI
                     NPLVVYEQGSGTRAVDVRIILSNG"
     gene            complement(32733..34628)
                     /locus_tag="PLW16_04865"
     CDS             complement(32733..34628)
                     /locus_tag="PLW16_04865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB70271.1"
                     /translation="MTKDPGLNEMAKWQDRYPEKFADLDPIFSHIRRGDHIFVASGCG
                     EPQYLVQALTDFVENHPKAFFDAEIIHVYSFGIAPYTNAKFKRNFRHNSFFIGNNTRD
                     PVNEGAADYTPISLSDVPALLYRGLIRIDVALVQTSPPDEHGYMSLGVSVDMVKAATD
                     VSKLIIAQVNREMPRIHGDGFIHIDDVNFIVPHDEPLLEVSGKTLSDVSGRIGNFVAR
                     LIQDGDTIQVGFGSLPNAIMANLYGKKHLGVHTELLSDGLVDLIKAGVIDNSRKTLGR
                     GKTVAAFAMGKRETYRFLHDNPSVLFRTIDFTNNPMNIARLDNIVAINSALEIDLTGQ
                     STSESIGGRFYSGIGGHYDFMRGAHFARNGKTILAMKSTARNETFSRIVPALKEAAGI
                     TLNRGDVHYVITEYGIAYLHGKNIRERSMSLIAIAHPKFRPWLIEEAKMRGLIYKDQA
                     FIPGKRGEYPEQLESYRTTRTGLQIFIRPVKISDEPKIKDFFYSLSDRSLNRRFLSQR
                     KDMPHERLQDFVVIDYTKEVLLLAVTGDEENELIVAVGQYGVDEATHSAEVAFAVRDD
                     HQNLGIGTELLSYLTYLAKREGLLGFTAEVLVDNKPMLHVFEKGGFDIKKKTEAGVYD
                     LQMAFRG"
     gene            complement(34755..35465)
                     /locus_tag="PLW16_04870"
     CDS             complement(34755..35465)
                     /locus_tag="PLW16_04870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB70272.1"
                     /translation="MSATSKTSSPQTLDARPCRAVVFDFDGTLARLNIDFVVMRSDVL
                     KLTAQYGLAVEAVCDLPALELIDRARATLAAVDPPRAEAYVAAAHARIMEIELAAARV
                     GELFGGTRKLLVALQKRGIGTAIITRNCLRAVRTVFPDLDQFVDTAISREMTPRVKPH
                     PDHLRTALAALAAPPDGTLMVGDHPLDMQVGREVGAFTVGVLTGSGNCDDLTRAGAQC
                     VLASASDLLDLLDQACPP"
     gene            complement(35425..36615)
                     /locus_tag="PLW16_04875"
     CDS             complement(35425..36615)
                     /locus_tag="PLW16_04875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB70273.1"
                     /translation="MNETAATPSCNNRISDRARTLRPFIVMDVLEKAQEKEKRGDSVI
                     HLEVGEPDFDTPECIKEACFRAVREGKTHYTHSLGLLELREAICKHYWLTYRVRISPD
                     QIIVTSGTSPAMLLIFAALLEKEDEVIISDPHYACYPNFIRFFDACPVSVDVSEEEGF
                     QYTPAGIREKLSTRTRAIMINSPSNPTGNLLGADRMADIAALGPPVISDEIYHGLVYS
                     GKAHSILEYTDRAFVLNGFSKYYAMTGWRLGYLIAPPEFIRPMQKIQQNLFISANAFV
                     QWAGVTALTSCGQCMRQMRNTYDERRQYMLERLRGMGIGVRVAPTGAFYILGNVKAFS
                     RDSYRLSFDILDKAGVAVTPGIDFGQNAEGYLRFSYANSLDNITEGMNRLERYLKNLE
                     PANP"
     gene            complement(36766..37614)
                     /locus_tag="PLW16_04880"
     CDS             complement(36766..37614)
                     /locus_tag="PLW16_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70274.1"
                     /translation="MDRGLLADRERFVNRIVSCLGRCELDYALPPSSGDADPVLPAGV
                     LFLLSFRTDPLSPDPDNGEFVLHLIKRSATVAQGGDLSCPGGILDPRRDPVGRCLLPR
                     LFPIIPRAVNRELARRDAATRRAVLLFLANALRESWEEVRLRPWQVRFLGALPPYSLL
                     LLKRRIFPVVGLLDGTWRPRINAEVERIVEIPLRAFFDPANYAWFDFSLPDGTSDHTG
                     MPAEFPCLLYREADGRLEVLWGATFNITMTFLKIAADFERPAIMRDRVVRRTLPPGYL
                     RGSNRS"
     gene            complement(37614..38687)
                     /locus_tag="PLW16_04885"
     CDS             complement(37614..38687)
                     /locus_tag="PLW16_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB70275.1"
                     /translation="MKRDRFKNVSTLAAEGSSKRRIDHFDPGPVIRNPHLQSILASLK
                     LRVLGPNPLVTAARELILDAGGGVRLQGFWSRHDPPTRGLVAMIHGWEGSAASAYMLS
                     CGRALFRRGFDVFRLNLRDHGDSHHLNKDLFHGARIDEVFQAVIAVAALSPGMPFFIV
                     GFSLGGNFALRIGLRAGNGSLPTLRRIFSISPALDPYKATLAIDRGPALYRHYFLHKW
                     KRSLRRKQALFPNCYDFSAHLSHTTLIGLTEALMSHNPEYENYTDYFRAYTLTDALLV
                     PLSVPVTMITSRDDPVVPVEDFYPLGNNARAEILIQPYGGHCGFMQLYPPGCWYEPWI
                     AARMLDELEEAFHRSRRNPGGRS"
     gene            complement(38936..39012)
                     /locus_tag="PLW16_04890"
     tRNA            complement(38936..39012)
                     /locus_tag="PLW16_04890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38976..38978),aa:Met,seq:cat)
     gene            complement(39059..40789)
                     /locus_tag="PLW16_04895"
     CDS             complement(39059..40789)
                     /locus_tag="PLW16_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPB70276.1"
                     /translation="MMKKLFSSVLIGLILLIPGALIGAAEQKSVAVLPFVVHSAESLD
                     YIKQGIWEMLASRLSSDGRLAVPSHDQIQDALGNFAGKELSQADVYELGKTMKTDYVV
                     WGSITKIGNSLSIDGKLVDVAAYKSPVGIFVQSQNLDEVIPRISDFAQRINTHITGVT
                     PEPQPAVAPPPEAPARVAAPPAGGDAREAAIIAGMRTSKKGTFTAAINPDFINAAQPL
                     DRRGFWMSPTSPTEFRGMDIGDVNGDRLNEVVLIDNYNVYIHQKTDAGFKLLQKISGK
                     RYDRYLAVDIADINRNGVNEIIVTCLRQTRLASFVIEFREGKFTPIASDLPWFLRVIG
                     TSSDSARLLGQEISPEHPFDTPIHKIVWENDRYREGQRMKIPQGLSVYGLALDDLGLG
                     SDEKIIAIDESDYLCIYEQTSKPLVKLAVFGGSKERLFKSDEVFGGSNVSIDLSSIQP
                     SAYQDEGRDRYFINPRILIYDTNGDKKPEIILVKNLSSTGRILKNVKLFTSSEVYNLE
                     WDGLGLIENWKTRKIKGYVADYQFKDIDNDGQNEIVLALVQSVGATIGQRSVIVAYKM
                     SAHYAPPVQQ"
     gene            complement(40900..>41414)
                     /locus_tag="PLW16_04900"
     CDS             complement(40900..>41414)
                     /locus_tag="PLW16_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB70277.1"
                     /translation="ACTHTFRGDLTVRFRAIKPSDEEEMRRLFYRFSDNMVYYRYFTS
                     IKTMPHGKMQEYVNVDFRRFMAIVGLIEEDGLERIIAEGRYVRHQDRPYADTAFIVDE
                     KYQGLGIATFLLELLIKYAQEKGIQGITADVLADNKPMLKVYEKLPYPIRAVMEFGIY
                     NLTIPFSEEA"
BASE COUNT         8170 a        12204 c        13353 g         7687 t
ORIGIN      
        1 cagaaaaatc ctcattgcaa accgggggga aatcgccctg cggatcctga ggacgatcaa
       61 ggaactcggc aagggggccg tcgtggttta tgagaaaccc gacagtgagg cctattacat
      121 ccggctcgcc gacaccgcca ttctcatcgg cgagggtccc cggtgcgatt atctcaacat
      181 cgaacggatc atctgggccg cccgcaagag cggggcggac gccgttcacc ccggatacgg
      241 gtttttggcc gagaacgcgg acttctccga ggcatgcgaa aaagcggggc tcattttcat
      301 cgggccgccc gccggggtga tccgggatct cggcaacaag gtcgtggcgc gacggattgc
      361 cgcccaggcg ggtatcgaca ccattcccgg cacgggcgat ctgccgcgcg gggaagacgg
      421 cgtggccgcg gcgctgaagt tcggactgga gcacggatac cccctcatgc tcaaggcctc
      481 gtccggaggc ggggggcgcg gcatccgcaa ggtggtcaac gagaaggacc tgctcgtcca
      541 gcttccaatg gcgcgccagg agacgctctc cgccttcaac gacgagaaca tctacgtgga
      601 gaaatgcgtc gaggcgcccc ggcatgtgga gatccagatc ctggctgatc ggcacgggca
      661 gatcattcat ctggggtcgc gggattgctc cattcaaagg cggcaccaga aacttctcga
      721 gatcgcgccg gcaaatctcc ccgtcgacgt tctggaggcg atgtatgccg cggccatcaa
      781 ggcggcccgc caggccggct acatcaatgc cgggaccgtg gagttcctcg tggatgcgcg
      841 gacgaacgcg ttctggttca tggagatgaa cacccgtctg caggtcgagc acaccgtcac
      901 cgaggagttg acgggcgtgg acatcgtccg ggagcagatc cgcgtcgccg aagggcaggt
      961 tctcgacatc ccgcgcgagc gtgttcacct catggggaag gccgtacagg tccgggtcaa
     1021 tgccgaggat ccgaagaacc acttcatgcc cgaggggggc aagcaggtgg aggtgtatca
     1081 gtctccgggt gggcccgggg ttcgcctcga cggcgtcgtc tatcagggct accgggttcc
     1141 caccgagtac gattcgctca tggtcaagat gacgatccgg ggctacaact gggagcagac
     1201 gatccagagg ctgaaacagg cgcttaatgg atttctgatc gtcggtcccg ccacgacggt
     1261 cccgttttat cttgccatct gcgacgagcc ggatttcctg gcggagcgct tcgacaccgg
     1321 ctacctcgac gcccatccgg ccgtcttcca gtatgcggag caggaacgcg aagtggccaa
     1381 gctcgcccgg ttgattgcgg agatccacgc ccgcaagatc aacccatttg cctattgaga
     1441 ggaacttccc catgacgcgg cgaacgcaaa acaacgacgc gcggatcacc ccgcaagagc
     1501 tgcgccgaaa cggcgtccgg caggtccttg aaaaaatccg ccgggcgggt ggttattacg
     1561 tcaccaatac ggagcgcgat ctgtcccagt ccgatttcaa gaaccggatc atgccccata
     1621 cgcagttcct cgtggccccg cagcgtaacg acgccgggtt cttctctatc gaaatcaccg
     1681 gcggcgcgtc cgtccatgtg gacatgctgc gcaagcagat gaatccgctc gaaaaactgg
     1741 aggtcctgtc cgccaacatg cccgacacgc tgttccagac cctgtgccgg ggtgtcaacc
     1801 tcttcgggta tcgtccctat ccggaaaacg tgatccgtct gacggtgcgg agcttcgcgc
     1861 gttatgtgca cgtctggcgg gtgttcgact ttctgaacca cgtttctaac atgatccccg
     1921 tcttcgagga ggtgcgcgag tccggtgcca ttctggaacc gtccatctgc ttttccacgg
     1981 gacccgagca cacggacgcc tactatgtcg gcaaggtggg ggagatcctc gcggtgaccg
     2041 gagaagacat cctcctgtgc atcaagaacc acggcggcct ggggacgccc aaacggatag
     2101 gggcgctggt gcgcgccatc cgcgagcggt acccggacct cgtcatccac tatcacggcc
     2161 acaacaccga cggcgccgat ttgggccgga tcgtcgcggc cgtgaaaaac ggggcgaaga
     2221 tcgtcgacgc gagcgaccac gccatgacgg gcttttacgg cccccccccg ctgctgacag
     2281 cggtggacat tctcagcgac gagggctacg aagcggccgg gctcaaccgc caggcggtga
     2341 tcgacgcctc gaacattctc cgcggcgagc gtgaacatta ccgagacttc gaatcgcagt
     2401 tcctggggtt cgatcccacg gctcagatcc acaagcttcc cggcggcgcc acggggtcga
     2461 gcttcgagca ggccgtcaag ggcggcttcc tgcaccagat gcccaacatc ctccagaacg
     2521 agctgccccg cgtccacgtg gaactgggga actggtggag cgtgacgccg ggatcccaga
     2581 tcctctggac cacggcggtg aacaacgtcc tgaagggggc gcgttaccgg gacgccaacg
     2641 acgacctgaa gagcctaatg ctgggccgct acggggaatt cccgttctat cgtccatcgg
     2701 attcaattta tgaggccgtg ttcgggaaga actggaaggc gatcctcgag cacgagggcg
     2761 gctatcagcg catcgaggac gtcgatctcg ccgtggagcg gaaggtcctg gagcaccgga
     2821 tcggccgcgc cgcgacggac gatgaattgg tcttgtatct ccagcacccg aacgatgccg
     2881 tcgatttttt caagttcgag gccaaatacg gaaagacctg ggtcctttct ccgaacatct
     2941 ggttccgaaa gggtggattc ggtctcggcg acaagttcga gatccctgac gcttacgggc
     3001 ggctgcacgc agtcgaaatc ggccccaagc gaaggacgaa ggcgggcgat gccgtgacct
     3061 atctcgtcat cgaccatcac ccggagccca ttctgacgga gctggagggc gagggcgtac
     3121 ccgcgaaaaa gaagatttcc ttcaccccga aggaaatcga ggcactggcc aaaaccggag
     3181 acgtccgctc ccagatcatc ggcacggtca gcgagatccc catatccgag ggagaagagg
     3241 tatccgcggg ccagattttg atcgtcctcg aggccatgaa gatgctcaac aacgtcattg
     3301 cagaaatcaa cggccgggtg gcggaaatac ttgtcgcccc cggggatcgc gtcgaggtcg
     3361 gcgacccgct aatcgtcgtc cggaccgagt gaccggggag gatgcttccc atcaaggcag
     3421 gtttgtgata agagcgaaag ccggtagcct ataccggctt ttttatggag tcctcgatga
     3481 tcgatgccat tctgcgaaaa atcgtgggca gcaagaacga ccgcgaactg aagcggctcg
     3541 ggtttctgct cgaagagatc aatgatctgg agccggtatt cgtggcgtta tccgacgccg
     3601 aactggccgc caagaccccc tgtttcaagg aaaaaatcga caatggggcc tcctgcgacg
     3661 acatcctgac cgaggcattc gccgcggcgc gggaggcggc gcgacggacc ctcggaatgc
     3721 ggccgttcga cgtccagatc atcggcggga tcgtcctgca cgaggggaag atcgcggaga
     3781 tgaagacggg ggaagggaaa accctcgccg cgacgctgcc cctgtacctg aatgccctga
     3841 cgggccgtgg gtgtcacgtc gtgacggtga acgactatct ggcccgacgc gacgccgaat
     3901 ggatggggcc ggtctacctg atgatgggat tgtcggtcgg agtcgtcgtc cacggcatgg
     3961 acgacaccga gcgccgagcg gcctatcatg ctgacatcac ctacggcacg aacaacgagt
     4021 tcggtttcga ctacctccgg gacaacatga aattcaccct ggatgactac gtccagcggg
     4081 actttcacta cgccatcgtc gacgaggtgg acagcatcct gattgacgaa gcccgaacgc
     4141 cgctcatcat ttccggaccc tcggaggagt ccaccgacaa gtactatcgg atcaaccagg
     4201 tcattccccg cctgaagaag gatcaggact acacgatcga cgaaaaaagt cgcaccgtcg
     4261 ccctgacgga ggacggcgtc gcccgtacgg aaaacgccct caaggtccag aacctctacg
     4321 aacccagaaa tatggagatc ctgcaccacg tcaaccaggc cctcaaggcc cacaccctct
     4381 tcaagcggga cgtggattat gtcgtgaaag aggggcaggt catcatcgtc gatgagttca
     4441 ccggccggat catgccggga cgacgctaca gcgacggcct gcaccaggcc ctcgaggcca
     4501 aggagcatgt caaggtcgaa cgggaaaacc agaccctggc ctccatcacg tttcagaact
     4561 atttccgcat gtacgagaaa ctggcgggca tgaccggcac ggcggacacg gaagcggcgg
     4621 agttcaagaa gatctacaac ctggacgtgc tcatcattcc gaccaacaag ccgatgatcc
     4681 gtcaggatca caccgacgtc atctacaaga ccgaggatga aaagtttgcc gcggtcatcg
     4741 acgagatccg cgagctgcac cgcgagggcc gtccggttct ggtcgggacg atctctatcg
     4801 cgaaatcgga gctcctgagc aagtacctga cccgtgcggg gatcaagcac catgtcttga
     4861 acgcgaaaaa tcacgagcgg gaggcggaga tcattgccca ggccgggcag cggtcggcgg
     4921 tcacgatctc caccaacatg gccggacgcg ggaccgatat caagctcggc gagggcgtgg
     4981 ccgatctggg cggccttcac attctgggca cggaacgtca cgagagccgc cggatcgaca
     5041 accagttgcg cggccgttcc ggacggcagg gcgacatggg gtcgtccagg ttttacctct
     5101 cgctcgagga cgacctgctc cggatattcg gtgcggagcg gatctcttcc atcatgaacc
     5161 gcgtcgggat ggaggagggc gagcccattg agcaccggct catttcccgg gccatcgaaa
     5221 acgcccagaa gcgcgtcgag gggcagaact tcgatatccg caagcatctg ctggactatg
     5281 acgatgtcat gaaccggcag cgccaggtca tctacgaaca gcggaaaaag gttctccggg
     5341 gggaggagct ttggaaggac atcgagtcga tgctcgccga ggtcatcgag gacaccctcg
     5401 cgcaatacat cgacgagaaa caccaccatg aaacgtggga cttgaagggg ctccaggact
     5461 gggtctggcg ccagtttggc ctcaagctgg acctggccgc cggggccggg gatcctttcg
     5521 gtgcggggac gatcggagaa accatcgccg ccgccgtcat gggcctgctc caggccaagc
     5581 agcgggagtt cggcaagccc ctgatggaat atctgatgcg gatgatcatg ctccatgcca
     5641 tcgacgccca gtggaaggac cacctcctgg gcatggatca cctgaaggag ggcatcgggc
     5701 tgaggggcta tggccagaag gatcccgccc gggagtacca gaaagagggc tacgacatgt
     5761 tcatggacat ggtgtggcgg atcaaggagg atacgctgca gaagctctgc atggtgcaga
     5821 tccggcggga agaagaggtg gaggagatga gggagcgcca gcggcaggat tacatcatga
     5881 gccggggcga agacacgccg gcctctcaga cggtgaggcg cgaggagaag aagatcggcc
     5941 gtaacgatcc ctgcccctgc ggcagcggga aaaagtacaa gaaatgttgc ggccgataat
     6001 cccgggggag ggcgacgcgg ggggaagatg gaacggaacc agacgtatat cgtgcccgga
     6061 tttaccgcga gtggaatcgc agcggggatc aaggcggacg gccgcacgga tctggccctg
     6121 atccgctcgg atgtgcccgc ggtcgcggcc ggcgttttca cgacgaaccg gttcccggcc
     6181 gcgccggttc agatcgaccg ggaacgtatc gcccggggag cggcacgcgc gattctcgcc
     6241 aacagcggca atgccaacgc cgccaccggg tccgacggct tgaaggacgc tctcgcgtcg
     6301 tcgcgggaac ttgcccgccg gctggcgata ccggaacacc agatcctcgt cgcctcgacc
     6361 ggcgtgatcg gccaccgcct gcccctggaa aagatcctcg ccggacttcc aaggctcgtc
     6421 gccgacctga gtcccgaggg cattccggcg gcggaagagg ccatcctgac gacggaccgt
     6481 tttccgaagc tcgccgtcgc ccggacgcgc tgcggaggca gggacgtcac cgtgtgcggg
     6541 ctcgccaagg gggccgggat gatcgaaccc cacatggcga cgatgctggc ctttatcctg
     6601 acggacgccg tcgtgctccg ggacgacctc gaccggcttt tccgccgcgc cgtgcgttcg
     6661 accttcaacg ccatcagcgt cgacggctgc gagagcacga acgacaccgc cctgatcctg
     6721 gcgaacggcg tcgcggcgaa ccggccgatc gcagccaggg gtgccgacgg acgccgattc
     6781 ggagctatgc tcaccgaggt catgggacaa ctcgcccggg acatggtcga ggacggcgaa
     6841 ggggccacga aacgcgtgga gatttgcgtc agcggggcgc ggacccgggc cgaggcgcgc
     6901 cggatcgcgt acgcggtggc ccgatcgaac ctcgtgaaga ccgcgttcta cggcggagac
     6961 cccaactggg ggcggatcat cgccgccgcc ggctccgtcg ggattgccct aacggcggac
     7021 cgggtgactc tccacttcga ggacgtcctg atgttcgccg acgggcaggg acgggcgccg
     7081 gatcccgagc gaattttcgc catcatgcag cagccgatca tccgtatccg cctcgaagtc
     7141 gccatgggca aagccgcgtt tgtcctgcag acctcggatc tcacccgcga ctatgtcgcc
     7201 ctgaacgccc actatcacac ctgaaaagat ttttgcttgc acaattcgga cctttatgct
     7261 atcagcctcc gcacttcgca catccccgga cgggccggtg tgtgcttttg aatgcaaaag
     7321 tcgccggccc gggcgaaggg gatggaggaa aaaacacaac aaaggagaac acgtatggcc
     7381 atcatttcca tgaagctttt gctcgaagcc ggggtccatt tcgggcacca gacgaacaag
     7441 tggaacccca agatgaaacc ctatatcttc ggggcgcgca acaacatcta catcattgac
     7501 ctgcagcaga ccgtcgagat gttccaggtc gcctacaatt tcgtcgtcaa gtccgttgcg
     7561 gaaggcggcg agatcctgtt cattggaacg aagaaacagt cgcaggaggc cattcgcgag
     7621 gaggccgagc gttgcgggat gccttacgtc aaccagcgct ggctgggcgg catgctgaca
     7681 aatttcgtga ccatcaaaaa gagcatcgac cggctcaatg ccctggacgc catgttcgcc
     7741 gacgacagca tcaaggcgtt tccgaagcgg gaaatcctca tgcggcagaa ggacaaggac
     7801 aaactggaca aggtgctggg gggaatccgc cgcatcaagg gccgtccagc cggggtgttc
     7861 atcgtggatc cgaagcgtga agacatcgcc gtgcaggagg caaacaaact ggggattccg
     7921 atcgtggcca tcgtcgatac gaactgcaac cccgacgtca tcgattacgt gattccggga
     7981 aacgatgacg ccatccgggc gatcaagctt ttctcgtccc gcgttgcgga cgccgtgctc
     8041 gaaggaaaga ggcgcttcga ggaacgcatc caggcggaga ccgacaaggc gtcggagcgc
     8101 accgccgcgg tcccggacga ggaaggcgaa aacgacgtcc ccgagagcgt ggaagcggtc
     8161 ggtgatgccg ccgtggccgt tcagccgacg ggggacaaga acgcaacaca cgaggaggta
     8221 gcataaattg gacattacat ccgcaatggt gaaggattta cggaccaaga cgggcgccgg
     8281 catgatggac tgcaaggaag ccctgacggc gtcggccggt gacgtcgaga aggcgatcga
     8341 atatctgcgc aagaagggca tgtcggcggc gacgaagcgg tcctctaagg ccgccaagga
     8401 gggggtggtg tcctcctaca tccacatggg cggtcggatc ggcgtcatgg tcgaggtgaa
     8461 ctgcgagaca gattttgtgg ccaagaccga ggatttccag gcgatgtcca aggatatcgc
     8521 catgcacgtg gccgcttcca atcccctctt tctgaagccg gaagagatcc cgcccgaggt
     8581 gatggaacgc gagaaggaga tctaccgttc gcaggcgatg gccgaaaaga aaccggagaa
     8641 gatctgggag aggatcatcg agggcaagct gaaaaaatac tacgaggaag tctgcctcct
     8701 ccagcagaaa ttcatcaaga acaccgacat caccgtcgag accctggtca acaatatgat
     8761 cgcgaaaacc ggcgaaaaca tcgtggtccg ccggtttgcc cgattccagc tgggtgaaga
     8821 cctgaagtag cgcttccgga ccgcggaaag gaaggcgggg ggatcatgcc gaaggggaag
     8881 gccgtttatc gacgggcgct gctgaaactg agcggagaag ccctgctggg tgcacaggcc
     8941 ttcgggatcg atccggagat cgtggccctg gtcgccgggg agatccgttc cgccgtcgcc
     9001 ctgggcgttc aactgggcgt cgtcatcggc ggcggaaaca tcttccgggg aggaggcacc
     9061 ggtgcccgcg gcatggaccg gacgaccgca gactacatgg ggatgctcgc gacgatcatc
     9121 aacagcctgg ccctgcaggg cgccctggag aagcagggcg ttcccacgcg cgtcctgacg
     9181 gccatcgaaa tgcgcgccgt cgccgaaccc ttcatccagc gtcgcgccat ccgccacatg
     9241 gagaagggcc gggtggtcat cttcggcggg gggacgggca atccgttctt cacgacggac
     9301 acggccgccg cgctccgcgc cgtggagatc caggccgagg tcatgatgaa ggccaccaag
     9361 gtggatggcg tttatgacca tgatccaaac cacaatccat cggccgtcat gtataaaatg
     9421 atcacctata acgaggtctt ggcgaaggat cttaaagtta tggatgcaac ggcgatctcg
     9481 ctctgcaggg agaaccgcct tcccattctg gtgttcaacc tgctcaaggc ggggggcatc
     9541 cggcgggccg tttgcgggga aaagatcgga acgatcgttc aggagtgaga catgaaggca
     9601 ttggtttacg acgaattgaa ggagaacatg gaaaaggccc tccagtccct ggaacggtct
     9661 ttcgccaagg tccggacagg tcgcgcgtct cttgggatcc tcgacgggat ccgcgtcgat
     9721 tactacggac agccgacccc actcaatcag gtcgccaccc tgtcggttcc cgagagccgg
     9781 ctgatcgtca tcgccccgtg ggacaacagc gcgatcggcg ccatcgaaaa ggccctgcag
     9841 aaggccgatc tgggattgat gccgacgaac gacggcaagg tcatccgcct gtccatcccc
     9901 accctcaccg aggagcggcg tcgcgaactg gtcaggaacg tcaagaagat ggcggaggaa
     9961 tgcaaggtta aactgcgcaa cgcccgtcgt gatggcaacg agcaattcaa gacgttgaag
    10021 aaggacaacg agatctccga agacgaaatg tacacgtgtc aggaagaggt tcagaaacgg
    10081 acggacaagg ccatcgaacg aaccgacgcc ctgctcgcgg ccaaggaaaa ggaaatcatg
    10141 gagatctgac ggttctccag aagatccgtc catccgtgtc ttgaaaaccc acccccgttg
    10201 tgctacaccc tcaacatgaa cccggaacgg agaatccgta gcctgcgtcc gcccggacag
    10261 ggaaggaatg cttgatggtc agtctcgatc gcgcccatct tccccggcat atcgccatca
    10321 tcatggacgg caacggccga tgggcgcagc gccatgcctt gggacgcatt accggccaca
    10381 aacgcggggc gagggccgtc cgcgtcgcgg tggaaacctg ccgagagctt ggcattggct
    10441 gtctcaccct gtatgccttt tcccaggaga actggcagcg tccaccggaa gaggttcagg
    10501 ccctgatgca cctgctcgag gactacctcc ggacggagat cggccggatg atcgagaacg
    10561 acatccggct cgtcaccatc ggggaaacgc aaaagctgac cgcgccggtc cgggccatgc
    10621 tctcgaacgg tatgcggaaa acggagaaga accgctcgat gatcctgaat ctggccctga
    10681 gttacggcgg aagggacgag atcgtggcgg ccgcgaagcg ggccatccgc gaggccgaag
    10741 ccggtcggct cgatccggac gcgctcacgc ccgaacggtt ttcccggttt ctcttcaccg
    10801 cggaccttcc ggatccggac cttctcatcc gaaccagcgg ggagtaccgg atcagcaact
    10861 tcctgctctg gcagacggcc tacaccgagt tctattttac cgacgtcctc tggccggact
    10921 ttcgccggaa gaaccttctg gaggcgatcg ccgactacca gcgccgggag cgacgcttcg
    10981 gtctgacgag tgagcagttg tctcgggagt agcggggcgg attcaggacc cgatccacgc
    11041 ccgaagcgcg acggtgaaaa ttttcagggg gggactccgc accctggggc ggaacgggac
    11101 gcagggggtc gaaggatcgc gcatgcacgg aggaaacgtg atttgcgcgg ggcgtgacac
    11161 ggccgcgacc acgggggggg aacggacatg ggcatggagc agcatctgca gcgatggatc
    11221 acgggcattg tcgccgtccc cgtcctcttt gccgtgatct tcttcgcccc ggtgcccgcc
    11281 ttcgcggcgg tcatcgccct gtttgcctta gccggcctgt atgaattcct ggcgatgacc
    11341 ctgccgcggg cgacggcccg gcagcggggg acggcgctgg cggcaggtgc gttgattctg
    11401 gcggcggcca ccgtcggcga tgccgccctg atcctcggcg tgatcaccct gagcgtgatc
    11461 tgcgttttcc tgctccacct gctgacgatt cgatcggagg cgtttgacct cgacccggtt
    11521 taccggatcg gcttcgggct tctctacgtg ccggtcctca tggcgcactt catcctgctg
    11581 cgtcagtctc cgcggggaat cgcgtggatc ttcttcgtca tcgtcctcgc gtttgccgga
    11641 gacatcgccg cctactatgt ggggcgtacc tgggggcacc ggaagctcca ggccgtcatc
    11701 agtcccggca aatccgagga ggggatcctg gggttggttc tgggcagcat tgccgggtgc
    11761 ctcctgtacc ggacgttttt cttcccgaca ctcggttggg agcacgccgc agtgattgcc
    11821 ggcgtcggcg ccgtcatcgg tcaattgggc gatttatgcg aatcgacgat caagcgggcc
    11881 tcgggggtca aggattccgg attcctcctg ccgggtcatg gcggggtgct ggaccggctg
    11941 gactgcctgc tctttatcac ccccttcgtt tactactacc agctctttgt gctgaaatga
    12001 aaacgatcgc catcttgggc tcaaccggct ccatcggtcg cagcaccctc cggatcgcca
    12061 ccgagcattc cggacgcttt tccgtcggtg cgctggccgc gggtcgcaac tggcgactgc
    12121 tcgcggagca aatccgggaa tttcgcccgc acctcgccgc tgttctcgac gacgaagtcg
    12181 cgcgcgacct caaagtcgcg ctgggccggg gaacgccgac ggacatcgta acgggcgagc
    12241 ggggctacgg ccaagtggcg gaactgagcg actgtcaaat cgtcgtttcc gcaatggtcg
    12301 gcgcggccgg gttgcgcccg accattgcgg cgattgcggc cgggaaaaca cttgccctgg
    12361 ccaacaagga aagcctggtc atcggaggtg cgctcgtcac acggctcgcc cgggaaaagg
    12421 gcgtccgtat cctgccggtg gacagcgagc acagcgccat tttccagtgt ctggaggcgc
    12481 atgcggtggg cgtccgccgc atcctcctga cggcctccgg gggtcctttc cgatcgcggc
    12541 cggccggtga actggcccgc gttacaccgg aggaggccct gcgccatccg aactggacca
    12601 tgggggccaa gatcagcgtc gattccgcaa cgatgatgaa caaggggctg gagatgatcg
    12661 aggcgaaatg gctcttcgat tgcgatctgg aacgcatcga cgttctgatc cacccgcaga
    12721 gcctggtcca ctccctggtc gaataccggg acggatcggt catggcgcag cttgccgtgc
    12781 cggatatgcg catccccatc gcctatgcct tggcgtatcc ggaacgcctg gaaacaacag
    12841 ccgggcgact cgatttgagt gccgcacctc cgctggaatt cttttcgccg gacgaggaga
    12901 gattcccctg tctggcactc ggacgtcagg ccggtcggac cgggggcacg atgccggctg
    12961 tcctgaacgc cgccaacgag gtcgccgtgg acgcctttct ccgaaaacgt gtgggtttta
    13021 tggatatccc tcgcctgatc gccgcggtta tggcgaaaca ccgtccggca gacgggtccg
    13081 gcctcgatga gattcttgag gccgatgcct gggcgcgcca ggcggccgcc gactggatcg
    13141 aagaggcgaa cccgggacag tgaaaccttc cgccgcgcct gcggcgagga aaggacggca
    13201 ttgtgcttgg catcagcatc atttccctga tcattcttct cggcatcctg atttttgtcc
    13261 acgaattcgg gcatttcatc gtcgccaaat cctttggcgt cggcgtgctc aagttctcgc
    13321 tcggtttcgg ccataagctg gtcgggaaaa agatcgggga aacgaactat ctgatttcct
    13381 ggattcccct gggcggctat gtgaaactgc tcggcgacgc ggaaggcgag acgctctccc
    13441 cggaagacga gaagcggtct ttcctgaacc agacggtctg gaagcggatg ggcatcgtcc
    13501 tggcgggacc cgttttcaat cttctgctcg cactcgtcgt tttcttcggc gtgtacctgt
    13561 ctggcgtccc cgccctgacg gcgcggatcg gcgatattca gacagaatcg gcggcggccg
    13621 ccgccgggct gagggccggg gataccttga tcagcatcgc gggccagagg gtcacctact
    13681 gggacgacat cgcgcgcgcg gtcgaaacgg ggaaggggac acccctggcc ttgaggatcg
    13741 atcgtggcgg aacggttttc gacattcagg tcaccccgcg acgcgtgaag gggaagaatc
    13801 tcttcgggga ggaggtcagc gcctacaaga tcggcatcag tccgtcgggt caggtggtga
    13861 cgaagcgcct gggacccgtg aatgccttgg cggcgagcgt ttcccagact tgggcggtta
    13921 cgaaactgac cgtgatcagc atcgtcaaac tcatcgacgg caccctctcc gccaaaaccc
    13981 tgggaggacc gatcctcatc gcccagatcg ccggggtaca ggcgaaggaa ggggtggtcc
    14041 ctttcctcct gttcatggcc ctgctcagca tcaacctggc ggtgctcaac ctgctgccga
    14101 ttccggttct ggacggcggc cacctgctgt tctacctgat cgaactggtc ttccggcggc
    14161 cggtcagcct gaaatggcgg gaacgcgctc agcaggtcgg attcgcgcta ctcatcatgc
    14221 tcatgatctg ggttttcatg atggacgtcg accgcctgca catcaagctg ttggatgact
    14281 tcgcccggat cttcacgaag cagccatgag aacgctcgcc atcgataccg ccggcatatc
    14341 ggctggcgtc tccctggcgg ttgacgggca gatccgctac gaggccttcc ataacacagg
    14401 cctcaaccat tccctcgttt tgctgccggc catcgaggcg gcctgtcgcc atgccggtgt
    14461 cgccctcgca gagatcgacc tcttcgctct gacgatcggc ccgggttcgt tcaccggtct
    14521 ccggatcgca gccgggaccc tcaagggact cgccttggcc gtgggcaaac cggtggtagg
    14581 cgtttcgacg ctcgattcac tggcctatcc gctcacgacg agccccgacg aggttctctg
    14641 cgcgatgctc gacgcccagc gggagcaggt ctatgccgcg gcctaccgga acggtgtgga
    14701 cggccggcgg gaaagaatcg tgcccgagcg ggtcgcggac ctcgacgaac tggtcgcctc
    14761 cctgccgaac ggaccgatcc tgttcgtagg cgatggcgcg gaacgccatc gcgatatgct
    14821 agccgcgcgc tcttccgccc tggtccgctt tgcgggtccc ctgtaccgcc acgtccgggc
    14881 cgccgcggtc gccctgctcg gagaggagcg ctaccgggcg ggggagtccc tcgatgtcgt
    14941 ccgcttttcc ccacgctacc tccgcgtttc tgaggccgaa cgacggcatt cctccccacg
    15001 ggaaagcacg aagaatgatt gacaacaccc gtcgatttgg ggtaagcgga ggccggaaac
    15061 cggccgggaa ggtcctgccg ggaaagggtt ggaaaaatgg ggaaaagaaa cgggatcatc
    15121 gttcgtgaag gacttccctt catcctgccg ctcgtcctgc tcacggccct tgcagtcctg
    15181 atgggcggcg gggcgtcggt cttcgtcctg ggtgcggtga cggtgttcgt catcgctttt
    15241 ttccggaatc ccgagcggga ggccccggcc gatcgaaagc tcgtgatttc accggccgat
    15301 gggcgcgtca ttcgcatcga gacggtcgac gcgcaggaac tgatccccgg gaccttgaag
    15361 aaagtcagca ttttcatgaa tgtgttcaac gtgcatgtaa accgggtccc ctatgacgga
    15421 accgtgagcg ccatccgcta ccggcccggc aagtttttgt cggccaatct ggacaaggcg
    15481 tcggaacaga acgaacggac ggcggttctg gtcagaacct gggacgaccg ggagattctc
    15541 acggttcaga tcgccgggct catcgcccgg cggatcgttt gctggctgaa ggaaggcatg
    15601 gcggtccggg cgggagagcg gttcgggctc atccgcttcg ggtctcgcct cgaagtcttt
    15661 cttcccgacg acgccaccgt tctcgttcag accggggaca aggtccgcgc gggacagacg
    15721 ccgataggac ggatgccatg aagaatccat cgcccttcag aagggacaac atgaaaaagg
    15781 ggatttacgt tctccccaac ctcttcacca cggccagcct gttctgcggc ttttattccg
    15841 cgatcgcctc catgaaggga ctgtttgagg tggcggccat cgcgatcatt ctggccgcgg
    15901 tcttggacgc cctggacggt cggatcgcac gcatgacaaa cacgaccagc aaattcggcg
    15961 cggaatacga ttccctgtcc gacctggttg ccttcggggt cgcgccgtcg ctgcttgcct
    16021 acaactggag cctcgccgct tacggtaaat ggggatggat ggccgcgttc ctctttacgg
    16081 tctgcggtgc gttgcgcttg gcgaggttca acatccagac ggggatcatc gaaagccggg
    16141 tgttcaacgg gctgccgatc cccggtgcgg ccgccgtcgt ggcgtccggc gtgctgatct
    16201 tctacaaatt ggggcaggag gggagattcg aaagccccct cgtgctgatc ggcgtcgtcg
    16261 ccatcgccat cttcatggtc agcaacatca aatactacag cttcaaggac ctaaatttct
    16321 tcgcccgcaa gcccttcatg tccttcgtgc tgatcgtgtt cgttctggcg gtcgtcattg
    16381 cggagccgca gatcatgatc tttacgttcg cgctgggcta cagcctgtcc ggtcccgcct
    16441 ggtggctgtt tcgtctgacc cgccgtgcct atgaaggatt caggcagcgt cggaccgaag
    16501 caacgaagac ggattgcaaa taagcgggga tgtcgggccg tgggcggacc gaaccgtgga
    16561 agcgcgacat ccgaagattg gagagtgagg aatgaaacag tacaattgcg ccgtggtcgg
    16621 cgccacgggt gccgtgggca atgaaatgat caaggttctg gaacaacgcc ggttccccgc
    16681 ggcggagttg cgcctgctgg cctctgagcg gtccctggga aagagccttt cgtatgaggg
    16741 acgggagatt cccgtcctcg tcttgaccga ggaatccttc gccgggattg acatcggcct
    16801 cttttccgca ggcggcgcca tcagcgaacg ctttgccccg gccgcggttc gggcgggttg
    16861 cgtcgtcatc gacaacacca atgccttccg catggagccg gatgttcccc tcgtcgttcc
    16921 ggaggtcaat cccgaggcga tcggacggta caaggaccgc ggcattatcg ccaaccccaa
    16981 ctgttccacc atccagatgg tggtggcttt gaaaccgatc cacgatgccg tccgcatcaa
    17041 gcggatcgtc gtctcgacct atcaggccgt ttccgggacc ggcaagaagg cgatcgaaga
    17101 actggccgcc cagacgcggg cgttgatcag cgggcgtgag gccgaggtca acgtctatcc
    17161 gcaccggatt gccttcaact gtctgccgca gatcgatgtt ttcctcgaca acggctacac
    17221 ccgtgaagag atgaagatgg tccgggaaac gaggaagatc ttcaacgatc cggaaatcgc
    17281 cgtgaccgcg acgaccgtgc gcgttcccgt gttttacgga cattccgaat cggtcaacat
    17341 cgagacggaa caaaagatca ccgccgcgga gtgccgggaa ctcttgagcc gggcgccggg
    17401 gatccgcgtg gtggacgatc cgggacagcg gtgctatccc atggccatcg atgcggccgg
    17461 gcgggacgag accctggtgg ggcggatccg ggaggacgaa tcgatcgcca acgggatcaa
    17521 cctttgggtc gtatcggaca acctccggaa gggcgcggcc ctgaacgccg tccagatcgc
    17581 ggaggttctg atccgggatt atctatgaga atcatcggcg gaacggctgg gaaacgcagg
    17641 atcctcgttc cgccgcgcgg gcggttgcgg ccgacggagg gccgggtcaa ggaggccctc
    17701 tttcagattc ttcccgacat gataggcaag gattttctgg atctgttcgc cggtgccggc
    17761 agtgtcggac tggaggcgat cagccgcggg gcgaagcggg tggttttcgt ggaaaaggac
    17821 cggcacctgg cggccgtcat ccggaaaaat ctggaactgc tggggtttgc cgccgcggga
    17881 acggtctggg accgggattg ggcgagcgcc ttgcggttgc tcgaggtccg gggcgatcgc
    17941 ttcgacgtcg tctttgccga tccgccctat gagcgggcct tcgccgaccg cgtcctgcgg
    18001 cggatggcga cgatcggggt tctaggggag ggcggatgct tcattctaca gcattcgcgc
    18061 cgcgaggacc tgccggatcc cggagagggt tggcttcacc aagtcgatcg caggatgtat
    18121 ggagacacaa ttctatcctt attttcagcc ccttaggaaa ggagaccgcc gcaatccatg
    18181 aaaaagatcg ccgtttatcc tggttctttc gatcccatta ccaacggcca catcgacatc
    18241 atcaagcggg gcttgaggat gttcgacgag atgatcgtcc tcatcgccta caatcccaac
    18301 aagaaatcgc tcttttccgt tgaggagcgt acggacttga tcaaggaggt tctgaagtgc
    18361 aacggccggg tgcgcatcga cagcttttcc ggactcctcg tggactacgt caagggcgcc
    18421 ggcgcgaatg tcatcctgag gggactgcgc gccctgtccg attttgaata cgaatttcag
    18481 atggccctga tcaaccgccg gttgaatcgg gacatcgaga cggtcttcct catgaccggt
    18541 tacaagtggt tctacacgag ctctaatatc atcaaggagg cggcgagtct cggcggttcc
    18601 gtcaagggat tggttccgga catcgtctat cagaaacttt tggacaaata cggaaatcaa
    18661 ccaagatgaa cttcgcacgc agaattcagc gcatcaaacc gtcgccgacc ttggccatca
    18721 cgatgaaggc caacgcgttg cggtcgcagg gacgggacat catcggtttc ggcgccgggg
    18781 agcccgattt cgacaccccg gagcacatcc gccgcgcggc ggtcgaggcg atcgagcagg
    18841 gattcactcg ctatacgccg gtcggtggaa tcgacgaact caaggacgcc gtcacggcaa
    18901 agctgttgcg ggatcagggc ctcgcctatc agcgctcgca gatcgtggtg tcctgcggcg
    18961 ccaaacacac cctctacaac ctggcccagg tcctctttga agagggggac gatgtcatca
    19021 ttcccgcccc gtactgggtg tcctatcccg acattgtgct cctcgccggg gcgaaccccg
    19081 tcattttgcc cacgcgcgag gaagacggct acaaggttca gccttcggac ctggtacgcg
    19141 cctttactcc ggccacgaag gccgtcatcc tgaacagtcc gagcaatccg acgggcgcgg
    19201 cctacagcga ggcggagttg cgcgccctgg gggacgtggt ccgggatcgg gacatttggg
    19261 tcatatccga cgacatctat gaaaagatcg tctatgacgg attccggttt catcacatgg
    19321 ccgcgcttga cgagcgcctg cgggcgcaca ccctggtcgt caacggtgtc tcgaaagcct
    19381 acgccatgac cggatggcga atcgggtatg ccgccggacc cgcggacgtc atcgccgccg
    19441 tcacgaagat ccagagccag aacacgtcaa atacgacctc gatctcccag aaggccgccg
    19501 ttgcggccct gaacggcgat cagggcgtgg tgggggccat ggtcgaggag tttcagcgtc
    19561 gccgggatgt catcgtggcg gggctgaatg ccatggaagg cgtgcgctgt cacacccccc
    19621 agggggcgtt ttacgtgttc ccgaacgttg cccgtctctt cggaaaatcc tatgacggcc
    19681 ggaccctgac cggctcggcc gatgtggcgg cctggctcct cgacgccgca aattgcgccg
    19741 tcgtacccgg aatcgccttc ggcgaagaca cctgcatccg actttcctat gcgacctcgc
    19801 tcgcgaacat cgaaaccggg ctggagcgca tcgcggcggc catcaaacaa ctctcctgaa
    19861 acgaggcaat catgggcgga ctattcgggg tcgtcaagac ggtcaactgt tccaagaccc
    19921 tgttttacgg aacggactac cattcccatc tggggacgga aaacggcggg ctggccgtct
    19981 gcggcgcaaa gggcttctat catacgatcc acagcatcag tcaggcccag tttaaatccc
    20041 gcttcgtgga cgactacaag gaaatggacg ggacgctcgg catcggctcc atcgacgatg
    20101 atgcgcccca gccactgatc atccgctcga aattcgggac gttcgccatc gcggccacgg
    20161 gtcaggtctc caacaagaag gccctcatgg cgggactcct tgatcagtgc gattccttca
    20221 atgaaatgac gctcggaggg atcaataccg tggagctcgt cgccaagctg atcagccggg
    20281 ggagcgatat cgcggacggg atcgagcgga tgcaggaggc catcgaggga tcgatctgtg
    20341 tgctcgtcat gacggccgac gggatttacg ccgcccggga ccgttacggg cgcttccccc
    20401 tgaccatcgg agaacgcatc ggcgggcgcg gaatggccgt ggcgaccgag gcgagcgcgt
    20461 ttccgaatct cggctatcag ctgtgtcact tcctgggtcc gggagaaatc gttttcctgt
    20521 ccgcggacgg cctcaaggtt ttgcgccccc cccgggagcg gcggcaggtc tgcgcctttc
    20581 tctggatcta taccggctat ccggcgtcca tctacgacgg cattggcgtg gagatggccc
    20641 gggagcgctg cggccgcgcc atggcccgga acgacgacat ggaagcggat ttagtcacgg
    20701 gcattcccga ctccggcgtg ggacacgcca tcggctatgc catggaatcc gggattcctt
    20761 tccggcgccc cctcgtcaaa tacacccccg gctacggtcg gagctacacc ccaccctcgc
    20821 aggacatccg ggaccttgtc gcgacgatga aaatcagcgc cgttcgggag gtcatccagg
    20881 ggagccggat gatcgtctgc gacgattcca tcgtccgggg gacgcagctg aagaatttca
    20941 cgatcgcgaa gctctgggaa aacggtgccc gggaaatcca tgttcggcct gcctgtcctc
    21001 ccctgatgta tccttgtatt ttcggctcct ccacccgaac cttcgcagag ctcgcctgcc
    21061 gccgggccat ccgggccatc gaggggcgcg acacggataa cgtggaggct tacctcgacc
    21121 gccggtcgga ggaataccgg agcatggtcg agtggattcg ccgggacctc aacgtgacat
    21181 ccctgaaata cctgccgatc gaagacatga tcgcggccat cggcctgccg gaaacggagc
    21241 tgtgcctcta ttgctggcgg ggtcgctgaa cgcgctccct ttttcaccgg aaacatctcc
    21301 catgcgcgct cgcgagatcc gttgtcctgg tggggcgaag cgcccttcaa ggcggcggcg
    21361 tgctgcgatc ccgcgcattt agacgttgat cggggccggt ttttcgtgat aggttaaggg
    21421 aaatgccggg gtggcccgat gacgcggtcg tagattgaaa tcagcgatgc gaagaaaaca
    21481 tacccagtcg gttttcatcg gcggtgttca ggtggggggc ggagcgccgg tcgtcgtcca
    21541 gtccatgacg aataccgata cgcgtgacgt ccggaaaacg gttcgccagg tccaccgcct
    21601 ggcacgcgcc ggatgcgaaa tcgtccgggt tgccgtaccg gatgcagagg ccgctcaggc
    21661 gcttcgggcg atccgaaaag ccagccggct tccgctgatc gccgacatcc atttccagta
    21721 cggacttgcc cttgcggcga tcaatgaagg ggtcgatgcg atccggatca acccgggcaa
    21781 cctcgcgcgg gataaaatcc gccggatcgc gaaggcggca ggtgaggcgg gcattccgat
    21841 ccgcatcggc attaatgcgg gatcgcttga aaaggatctt ctggcccaat acggcggacc
    21901 gaatgcaccc gcgctcgtgg aaagcgccct ccgacacatc gatgtccttc gacagtcggg
    21961 cttcgaagcc atcaaggttt ccctaaaatc ttcggatgtc ctgaccaccc tcgcggccta
    22021 tcgtgagatg tccgcccgga gcgactgccc ccttcatctc ggggtgacgg aggctggaac
    22081 ggaggtgcag gccgccgtca agtcgtccct cgggatcggc atgctgctcg cggagggcat
    22141 cggcgacacg attcgtgtct cggtgaccgg cgatccggtc cgtgaaatgc cggttgccta
    22201 cggcattctg agggcgctcg gcatccggag acgcggcgtg gagattatct cctgcccgac
    22261 gtgtgggcgc tgcgagatcg atcttgtgac gttgacgaag cggatggaga aggcccttgc
    22321 cggctgcacc acccccttga aggttgccct gatgggctgc gtcgtgaacg gtcccggcga
    22381 ggcggcggag gccgatgtcg gaatcgccgg cgggcgggga ttcgggtttc tcttccggag
    22441 ggggaaggtg atccgcaagg tcccggaggt ggagttcgtc acggccctgt tgacggaaat
    22501 cgatgcgctc gccgaacggg gagacgctta acacaaggag gttcaatgcg ttattcggag
    22561 ttgtttctgc cgaccgggcg ggaggtgccg tcagacgccg agctggcgag tcaccagctc
    22621 atgatccggg cagggatgat ccggaaactg accagcggca tctattccta ccttcccatg
    22681 gggtaccggg tgatccgcaa ggtggaacag atcgtacgcg aggagatgaa ccgggccggc
    22741 gcccaggaac tcttcatgcc gatggtccag ccggcggagc tctggcagga atcgggccga
    22801 tgggtccatt acggccggga gctgcttcgc tttcgggatc gccacgagcg ggagtattgc
    22861 ctcggaccca cccatgagga agtcatcacg gacctggtcc gcaacgagat caagacctac
    22921 cgccaacttc cacggaacct ctaccagatc cagacgaagt tccgcgacga gatccgcccc
    22981 cgcttcggcg tcatgcgctg ccgcgagttc gccatgaagg acgcctacag cttcgacgcc
    23041 aacgaggccg gtgctgagga gagctaccgg aagatgttcg aggcgtacgg ccgcatcttt
    23101 cgccgctgcg ggctcgactt ccgggccgtg gaggcggatt cgggaagcat tggaggaagc
    23161 tactcccatg aattcatggt gatggccgat tccggcgagg acgccgtcgt cttctgtaca
    23221 gcctgcgact atgcggccaa tctcgagaag gccgagatcg ctccgccgga gaaaaccccc
    23281 tccgtcgatg aagcccggct gcccctcgag gaggttcaca ccccaggcgt acggaccatt
    23341 gaggaggtct gcgcctttct gtcggtgacc ccccaggagg tcgtgaagac cctcatcttt
    23401 aacgccgacg gcaaacccgc ggccatcctt gtccgcgggg atcacgacgt caacgagatc
    23461 aaggtgaaga acgtcctggg ctgcgacgag ctggaattgg cgatggacga tatgatcctc
    23521 gacgtgaccg gctcgccgcg gggctttgcg ggtgcggtcg ggatccgggc gcgcgtgatc
    23581 gcggattact cgctgatcaa catgacgcac tgcgtgatgg gcgcgaaccg ggaggactac
    23641 cacctgaaaa acgcgagtcc ggaacgggat ttctccgttt cggcctatgc cgacttgcgg
    23701 gtcgtccgcg cgggcgatcc ctgtccgcgc tgcgccgccc ccgtccggat ggcccgcggc
    23761 atcgaagtgg ggcatgtgtt caagctcggc acgaaataca gtcggagcat gcgggccgtc
    23821 tatctcgatg cggcgggcaa ggaacagacg atggtcatgg ggtgctacgg gatcggcatc
    23881 ggccgcaccg tcgccgccgc catcgaacag agccacgacg atcagggaat cgtctggccc
    23941 atgacgcttg ccccctacgc ggtcatcatc acgccggtga acagcaacga ggaaaccctt
    24001 gctcgggcct cagaatccct ctatcaggca ctgtcggcca ggggggtgga agtccttctc
    24061 gacgatcggg acgagcgggc gggggtcaag ttcaaggatg ccgaccttat cgggatcccg
    24121 ctccggatca cggtcggacc taaaaaactg gccgaaggcc gcgttgaact ccgcatccgc
    24181 cggacgggcg aggtccggat gcttccactc ggcgaggcgg tggacggggt cagtgacttg
    24241 atccgcgccg agttggagag gatcggcgga tagaccggcc ggaaggcaga acgtaaacca
    24301 tgctgcgcat caccgacatc atcgacaagg cccaggcgta tcttgcgccg gcggagctcg
    24361 agatgatcga gaaggcctat gtcttttcag cctccgttca tcaggggcag gtccggctgt
    24421 ccggggaacc ctacctgacg catcccatgg aagtggcggg gatgctggtc gacatgcgga
    24481 tggacgcctc aacgctgatc acgggtcttc tgcacgatac ggtcgaagac accctggcca
    24541 cgccggagca gatcgaggag aacttcgggg aggaagtggc ctttctcgtc agcggcctga
    24601 ccaagatcag caagatcacc ttcggcagcc aggaagagcg gcaggccgaa aatttccgca
    24661 agatgatgct ggccatgtcc acggatatcc ggatcctggt cgtccgcctc gccgaccgga
    24721 tccacaacat gcgcaccctg cagttccaga cgcccgaaaa gcaacgctac atcgccaagg
    24781 aaaccctcga cctctacgcc ccgctcgcta atcgcctcgg catcaactgg atgaaggtgg
    24841 aactggagga cctctccttc gcctatctcc aacccatggc ctacgaagag atcaaacggc
    24901 gaatcgccat gaagaaggac gagcggaacc agtacaccga aaaggtccgg aacgtcctct
    24961 accaggagat ggaacgcttc ggcctgaagg gtgtggtgga aggcagggcg aagcatttct
    25021 acagcatcta caagaagatg gaagaacagc acatcgattt tgacgaggtc tacgacctca
    25081 tcgccttccg gatgattctc gatgcgaacc gggagaagga gtgctacgag gccctgagcg
    25141 tcgtacacgc cctctggaag cccgtgcccg gccgcttcaa ggactacatc gccatgccga
    25201 aggcaaacaa ttaccgctcc ctccatacca ccgtcatcgg cccctacggg gaaagggtcg
    25261 aggtccagat ccggacacgc gagatgcacg agtgggcgga ggaggggatc gcggcgcatt
    25321 ggcgctacaa ggaggggcgg tcccgggggg ggaaggacga cgatgagcag gtccgcaagc
    25381 ttcgggaact cctcgaggtt cagcaagagc tcaaggaccc ccgcgagttc atgtccaatc
    25441 tgaagcttgc cctgtttccc gacgaggtct acgttttcac gccgggcggc gatgtccgct
    25501 cttttcccaa gggggcgacg ccgatcgact tcgcgtacag cgtccatacg gatgtggggc
    25561 atcaatgcat cggggcgaag gtgaaccgca gcatcgtccc cctcaagtac cagctccaga
    25621 acggcgatac ggtcgaaatc attacccaga gcggccacca cccgagcaag gactggctga
    25681 agtacaccgt gacttcgcgc gccatctcca agatccgggc gtggatcaag accgaggagc
    25741 gcaaaaagag cgtcacgctg ggcagggacc tcctggagaa ggaattccga aagcaccacc
    25801 tcaagttcag ccagatcgcc aagagcgacg aattgaagaa aatctatgag gagcactcgc
    25861 tgaactccct ggaggacctc ctggccatcg tcggttacgg gcggatctcg ccccgccatg
    25921 tggtcaacct gttcctgcct gaagaggatc tcaaggagga ggcggttccg gaaaagatca
    25981 ggcgcaaggc gacgagccac gaggatttcg ggatttcgct cacgggggtg gaggatgtga
    26041 tggtgcgctt tgccaagtgc tgcaacccca tcccaggcga cgagatcatc ggctatatct
    26101 cccgagggcg cggaatcacc gttcatacgg ccaattgtcc aaacttccgg gatatggaca
    26161 ccgagcgtct ggtagaggtc aagtggagca tacgggagaa gcacacctat ccggtgtcgc
    26221 tgcgcgtcgt ctgccaggac aagaagggca tcctggccga gatcagttcc gtgattgccg
    26281 gcatggatgt caacatcagc caggccgaag tggaaacaca ctccacggaa atgcaggctg
    26341 tctgcaactt tcggatggac gtgaatgatt tgcagcagtt caaccaggtc gtggccgcgc
    26401 tgaaaaagat cacggaggtc ctgtccaccg agagggtccg cggggtctaa gcgacaaacc
    26461 gtgttgacgt cggcgggcaa ttgcggtaag aagccaacgg ctttgtaggc atcgaggaga
    26521 cgttattgca tgcaaagagg ggaaggaatg cgacatccgt tgggcaagat ccggttctgt
    26581 ctcgttgcgg cggccctgct gctcctggtc gcggtagccg cgtggcggcc gtgctgggcg
    26641 gcgccgcttg tcctgatcga tccgggtcac ggcggaaacg agcgtggcgt tcgtctgtcc
    26701 gacgcggtgt acgaaaagga cgtgaccttc gcgatcgccc ggatggtgcg gcaggacttg
    26761 atcgagtcag ggaggatcgc cgtgcagttg acgcggacgg aggatcgccg gatttccatt
    26821 caggaacgcg ccgacctggc cgcccgtctc aacccccgtc tcctgatcag tctccacgtg
    26881 aatgcgggct tcggcaataa atccaacggc tatgaactca ttttccccgg gttcggaacg
    26941 accgcgcccg acggccgggc cccgcaggcc gctgcccgct tcacggcgaa gcatgcctac
    27001 ctgaatgagg gtgtccgttt cgcgcaggtg gccgcccgtc acctggagcg ggtttttccg
    27061 cggaagggcc gggggctgcg ggaggccccg attcccctgc ttgaaggcct gacggtcccc
    27121 gcggtggtgg tggagatcgg ttttgccacc cacccggaag aaaggaaaag gctcctagat
    27181 cccgccatac agaaagcgat cgcacgggcc ctgtccgtcg ccctctccga atatttccga
    27241 tgaagatcgt cctcgcctcg cggaaccggg gaaagctcag ggaaatccgg gatcttcttg
    27301 acgggctggg aatcgaattc gtctccctgg cggacttcga ccatccgccg gaggtgatcg
    27361 aggacggcga aaccttctac gacaatgccc tgaagaaggc gcgaaccatc gccgagtacg
    27421 cgaactgcac ggccctggcc gacgattcgg ggctggaggt ggaggcgcta cggggggcgc
    27481 cgggcgtcca ttcggctcgt tatgccgggg ccggggcgga cgacctcagc aacatggaga
    27541 aactgctccg ggacttggag ggggtgcctc cggaaaaacg gacggcgtcc ttccgctgcg
    27601 ttgtggtcct ctgtcatcca gacggatgcc atcaagcctt cgaggggcga tgggatggac
    27661 ggatcagcga ggccatgcgg gggcgcaggg ggttcggcta cgatcccgtc ttttatctcg
    27721 acgaggcggg aaagaccgtt gcggaaatgt ccccggagga taagaaccgg cggagccacc
    27781 gcgcgcaggc gatccggaag ctgaagcgcc acctggagga cgtccgtggt gcgcatggaa
    27841 taaaatgacg gggcgtagcg cagcctggta gcgcgcctgc tttgggagca ggatgtcggg
    27901 tgttcaaatc accccgcccc gaccagttca ttgtcggact ttccaccatc ccgctgctcc
    27961 tctgaccggg aggcgtcgcc ccggcggcgt cttcggtcga accgctcccg acgacgcccg
    28021 tcatcgatgt gatccgcgca tccggtgggg ccctcatcct cgcaatcgga ttcaacatca
    28081 cgggaatcgc cggcttccgg tgggcaatct gccccccgca atcctcttca acgccgccat
    28141 ctgtgggctg ctcggatagg gttccgcttt aggcgctcat gatttcctgg agcgtgcctt
    28201 cgatcagatt tcggatgtag gccaggcgtg cctcggtctc ggcctcgaag cgcaagacga
    28261 ggacgggctg cgtgttggag gaacggatca gaccccagcc gtcggcgaaa gggatgcgga
    28321 cgccgtccac gtcgatgatg ggatagtctt ttcgaaaccg ctctttgacg gcctcgacga
    28381 tctgaaactt tcggtcgtcc gggcagtcga tccggatttc gggcgtgacg aaggttttgg
    28441 ggacgtcggc cagaagatcg ctcagacgcc ggccggtctt ggagaggatc tccagcaggc
    28501 gcagggaggc gtagatcgca tcgtcgaaac cgaaatagcg gtcggcgaag aacaggtgcc
    28561 cgctcatctc gccggccaga agggcgtttt cctccttcat tttctccttg atcagggagt
    28621 gtcccgcctt ccacatgatc gcgcggccgc cgtggcgctc gatgtcgtca tagagcttct
    28681 gggagcactt aacctcgccg atgatggccg caccgggatt cttttcgagg atgaagcgtg
    28741 aaaaaaggag gagaagttcg tcaccccaga ggattttccc ctggtcggtg attacgccca
    28801 agcggtcggc gtcgccgtcg taggcgattc ccgcgtcggc gccgcttgcg ttgacgcggg
    28861 cgatgagctc ctgaaggttt tcgagcacgg tcggatccgg gaaatggttg ggaaagcgcc
    28921 cgtcgggatc gcagtagatg tcctcgatcc gacagccgaa acgacgaagc agcggcaggg
    28981 cgaacaggcc gccgaccccg ttgccgccgt ccacgaccac ggagagcccc gaagtgatct
    29041 cgacgttccc gtaaagatgg tcttcatagg gctgacgaat gtcggccgtt tcggcccggc
    29101 ccgcgccggt ggcgtatcgc ccgttttcca ttagggtgcg gaggcgctga atatcgctgc
    29161 cgaagatggt gtccgggccg acgcagatct tgaagccgtt gaagtccgga ggattatggc
    29221 ttcctgtgac catgaccccg ccgtctgtgc cgaggtggcg gatggagaag tacagcatcg
    29281 gcgtggcgca caggccgacg tccagcaccg tcatgccgcc gtcgatcagg ccgcgcatga
    29341 cggcggcctg atactccgct gaactgaggc ggcagtcccg ccccgccgtc atgacctttg
    29401 ccccgcgctc gcgagccagg gtggcgatgg cccgacccaa gccgtaaatg aagtcgggat
    29461 tgagatcgcg acccacgagt ccgcggacgt cgtattcgcg aaagacctct ggattgatgg
    29521 taggggacat tcccatggcc aggtcccttc ttcaggcgaa atcgagcgcg gcgagatcga
    29581 cgtgttttct gaagacctcc tcgatcaggg cgattttctc ctgatcgtcc cgtgtggcca
    29641 aggtttcgat tccgtcgatc tggcgtgcgg cgtcgtacgt gtccgaggtg acaaagagca
    29701 gcggaatgcc gagttcctcg gcgcgggaaa tgagatgcgg cgcgggcaga atgttgttag
    29761 tcaggagcac ggccgcggca tgggcgtcga gtgcggccag aagcatgtcg ctacggtcac
    29821 cggttgtgag gacgagagtg ccgggttcgt gaaatagcgg attccggacg gcgacttcgc
    29881 aagacgtgga gccgatgaaa atgtctcggg ccacacggtt gaggttcgcc tcggcgctca
    29941 ggaccttggc gaagaggcga tcggcgaggt agccgacggt tcggtaggtc aggcgctttt
    30001 catatgggat cacacctagg atggggattt ccagctgagc gagcttcgga agatgcgtgt
    30061 cccgaaaatc gtcgatgttg gggacgttgt taatgaggac gccgcggaag cgcccggcgg
    30121 taaggccgag gcgctttttg agaaacgtca cgtcatcgag gaccaggtcc ccgtccccgc
    30181 tgacgacgac cagccaatcc gcatccagtg cctcggcgac ggagagcgga tcgaggtgga
    30241 cggaaacacc gtagctcaag tcccgaccgc cctcgatgaa aacgagatcc ttgttttcgc
    30301 cgatgttggt caggagttcg ccgagcttct cccgggtcgc ctgctcatcg agcatgtaca
    30361 ggagtttggc atggtgaaac ccgatgctca tgtcttcggg gttttcctcc aatttgaaca
    30421 ggctggtgat gagggcggca tcgtagtccc agagcctctt cttgcgatag aggaggcgtt
    30481 cgccgaaggg cttcatgtag ccgatcttct ttccaagcgc ctttgccatc ccgatgatga
    30541 cgctggtttt ccccgcaccc tgcctcattg aagccacgac cagtttgttc atggatctca
    30601 cctctcgcta tccgttggaa agaatgatcc ggacgtccac cgccctggtt cccgagccct
    30661 gttcgtagac gaccagagga ttgatttcga tgtcggatac gcccctgcac ttctggatga
    30721 tcgcgccgag cttgatcatc gtgtcgacca ggctgtcgat gtctttcgtc tcttctcccc
    30781 gcacgccgag caggagcggg taggagcgga tcgatttgat cattccgagg atctccttgc
    30841 gttccagggg cagggaacga aaggagacgt ccttcatcac ttccacatag atgccgccca
    30901 ggcccaccat caggatcggc ccgaaagccc gatcgatccg ggctccgacg atcatctccg
    30961 tgccgggacg gaccatctcc gagatctcga cgccttcgag gacggcatgg gggacgcttg
    31021 ttcggcaatt tctcataatg gcgccgtagg cgtccatgac ttcgtcggcg ttgtccagat
    31081 cgagggcgat ccctccggca tcgctcttgt ggatgatgtc gcgcgagacg atcttcatga
    31141 cgacgggata gccgatctgg tcggcgctcc ggatggcgcc gtcgaggttc tgggccgtga
    31201 ggctgttggg gatggcgatg ccggccaagg tcatgatggt gcgggcttcg tgcgacagga
    31261 ggaagtatcg gccctcacgc ctgacgtccg cgacaatccg gtcgatggct tcgacgtcga
    31321 tggtcgcatc ggcaatggcc tcgggaggtt catgcaggta gcggtgatat gcatacatcg
    31381 ccccgagcga cgcggcggcg ggatacacat cgtcggaaac aggaatgcca cggcgacgcg
    31441 cggacgcgac gtaggtggag gttttctcgc cgccgaagag ggagaatatg atcggcttgc
    31501 cggcggcctt gaagcggcgg tagttgtctt cgatcgcctc cttgaggtta tcggaactga
    31561 acagggccga ctcgcagtag acgccgagaa cggaatggat ctccgggcac ttcagggcgg
    31621 catcgaaggc ccgggtgtaa tcctgggccg tcgcctgacc ggtgatgtca atcggattac
    31681 gggtcgatcc gaagcttggt gtggccgacg tgaagacgtc cttgagaagc tgggcgtcgt
    31741 cgagcatgcc gaggccgaat ttttcgaagg catccgtcgc catgacgccc gccccgccgc
    31801 cgttggtaat gatgacggcg ttctccccgg cgggcagcgg gctgtcggcc aggaacttgc
    31861 tccaggcgaa ggcttcgctg gtgctctcgg cccggagcgc accgcactgc cggatgatgg
    31921 cgtcgaagac gtcgtcggcg cccgcgagcg acccggtgtg ggaggcggct gccatcgccc
    31981 cgcgggatga tcggccggac ttgatgatca cgacgggttt cagccttgcc gcctgcttca
    32041 gaatgcgtac cagcttctcg ccgttccgga tcccctcgat gtaaatcagg atcacgcggg
    32101 tgtcgttgtg gcgggcgaga tactgcagga ggtcggcctc gtcgatgtcg gccttgttgc
    32161 cgacggagac gatcgccgac atcccgatat tttccacttc ggcctgtccg atcatggtga
    32221 gccccagggc gccgctctgg gtaatcatgg cgaggtgtcc ggaggagatg ttcccgtcga
    32281 cgaacgtggc gttcaggttg acggcggagg aatagacgcc gaagacgttc ggcccgacga
    32341 tccgcatgcc gtgcccgttg gcatagtcga tgatcctgtt ttcctcttgc cggttgccga
    32401 tttcggagaa cccggaggta atgatgatgc cgtatttcac gccgcggtcg gcgcactgac
    32461 aaacggcgtc gaagacgaac ttggccggga tggcgatgat ggccaggtcc acctcgccct
    32521 cgatgtcggc cacccgggga tagacgggcc ggccttcgat gacgccccct gccggattga
    32581 ccggaaggat gcgtccggtg tagcctcccg aaacgatgtt cgcgaggacg gccgaaccga
    32641 tttttgcacg ctcatgggag gcgccgatca cagcgacgga tcggggttcg aagagatacc
    32701 ggatgcaatc gtcatggatg tcggacatgg gatcaccctc ggaaggccat ctgcagatcg
    32761 tagacacccg cttccgtttt tttcttgatg tcgaagccgc ccttttcgaa aacgtgcagc
    32821 ataggcttgt tgtcgaccag cacttcggcc gtaaagccga gcagaccttc ccgcttggcg
    32881 aggtaggtca ggtacgagag gagctcagtg ccgatgccga gattctgatg gtcgtcccgc
    32941 acggcaaacg cgacctccgc cgaatgcgtg gcctcgtcca cgccgtactg accgaccgca
    33001 acgatgagtt cgttttcctc atcgccggtt acagccagca gcaggacctc cttggtgtag
    33061 tcgatgacca cgaagtcctg caggcgttcg tggggcatgt ccttgcgctg ggatagaaag
    33121 cgccggttca ggctgcgatc cgacagcgag tagaagaaat ccttgatttt cggctcgtcg
    33181 ctgatcttga ccggacggat gaagatctgc aaacccgtcc gcgtcgtccg gtagctctcc
    33241 agctgttcgg gatattcgcc ccgcttgccg ggaatgaagg cctgatcctt gtaaatcagc
    33301 ccgcgcatct tcgcctcttc gatcagccag ggtcggaatt tggggtgagc gatcgcgatg
    33361 agggacatgg acctttcgcg gatgttcttg ccgtgcagat acgcgatccc gtattcggtg
    33421 atgacgtaat ggacgtcgcc cctgttcagg gtaatcccgg ccgcttcctt gagcgcgggg
    33481 acgatgcggg agaaagtttc attgcgggcc gtcgatttca tggccaggat cgtcttgccg
    33541 ttgcgcgcaa aatgggcgcc gcgcatgaag tcgtaatggc ctccgatgcc gctgtagaag
    33601 cgacctccga tcgattcgga cgtcgactgc ccggtcaggt cgatttccag ggcgctgttg
    33661 atcgccacga tgttgtcgag ccgggcgatg ttcatggggt tgttggtaaa gtcgatggtg
    33721 cggaagagga cggaggggtt gtcatggaga aaccggtacg tctcgcgctt gcccatcgca
    33781 aaggccgcca cggttttgcc gcgtccgagc gtcttgcgcg aattatcgat gacgcccgcc
    33841 ttgatgagat cgaccaggcc gtcgctcagg agctccgtgt gcacgcccag gtgttttttc
    33901 ccgtagaggt tcgccatgat ggcgttgggc aggctgccga aacccacctg aatcgtgtcc
    33961 ccgtcctgga tcaggcgggc gacgaaattg ccgatccggc ccgacacgtc ggagagggtc
    34021 tttcccgaca cctcgagaag cggttcgtca tgtggcacga tgaagttcac gtcatcgatg
    34081 tggatgaatc cgtcgccgtg gatgcgcggc atctcgcgat tgacctgggc gatgatgagc
    34141 ttggagacat ccgtcgccgc cttgaccatg tccacgctga cgccgaggct catatacccg
    34201 tgttcgtcgg gcggagaggt ctggacgagg gcgacgtcga tgcggatgag gccgcggtag
    34261 agcagagccg gaacatccga cagggaaatc ggcgtgtagt ccgcggcacc ctcattcacg
    34321 ggatcccgag tgttgttgcc gataaagaag gaattgtggc ggaagttccg cttgaatttc
    34381 gcgttggtgt agggggcgat gccgaaggaa tagacgtgga tgatctcggc gtcgaaaaag
    34441 gccttcgggt ggttttcgac gaaatccgtc aaagcctgga cgaggtattg gggctcaccg
    34501 catccggagg cgacgaagat atgatcgccc cgacggatat ggctgaaaat agggtccaga
    34561 tcggcaaact tttcgggata gcggtcctgc cacttcgcca tttcatttaa ccccggatcc
    34621 ttcgtcatgc ccttgattct ccgagccata gaataggtgc agcgctcgta ctgcgcaatt
    34681 caataaacac gaacggcgag tttgtgtcaa tagaaaagcc ggcgcggacg caccgccccg
    34741 gcggggaagg gcgctcaggg ggggcaggct tggtcgagga ggtcaagaag gtccgacgcc
    34801 gacgccagaa cgcactgcgc cccggcccgg gtgaggtcgt cacagttacc ggatccggtg
    34861 aggacgccga cggtgaacgc gccgacctcg cgtcccactt gcatgtccag tggatgatcc
    34921 cctaccatga gggtgccgtc cggcggggcc gcaagggcgg cgagtgcggt gcggaggtgg
    34981 tccggatggg gcttgacgcg cggcgtcatt tcccggctga tggcggtatc cacgaactgg
    35041 tcaaggtccg ggaaaaccgt ccgcaccgca cgcaggcagt ttcgggtgat gatcgcggtc
    35101 ccgattccgc gtttctggag cgcgaccaga agcttgcgcg tgccgccgaa aagctccccc
    35161 acgcgcgcgg cggcgagttc gatctccatg atgcgggcat gggctgcggc gacataggcc
    35221 tcagcccggg gagggtccac cgccgcgagc gtcgcccgcg cccggtcgat cagctccagc
    35281 gcagggaggt cgcaaacggc ttccacggcg agtccgtact gggccgtcag tttcaggaca
    35341 tcggaccgca tgacgacaaa gtcgatgttc aatctggcca gggttccgtc gaagtcgaaa
    35401 acgaccgcgc ggcagggacg ggcgtcaagg gtttgcgggc tcgaggtttt tgaggtagcg
    35461 ctcaaggcgg ttcatccctt ccgtaatgtt gtccaaggag ttggcatagg agaagcggag
    35521 atacccttcc gcattctgcc cgaaatcgat cccgggggtt accgcgacgc cggctttgtc
    35581 caggatatcg aaggacagac ggtaggagtc gcggctgaag gccttgacgt ttcccaggat
    35641 atagaaggcc ccggtcggtg cgacgcgcac gccgatcccc atcccgcgga gccgttcgag
    35701 catgtactgc cgacgctcgt cataggtgtt tctcatctgg cgcatgcact gcccgcagga
    35761 ggtcagtgcc gtcacgcccg cccactgaac gaacgcgttg gcggagatga agaggttctg
    35821 ctggattttc tgcatggggc ggataaattc ggggggagcg atcagatagc ccagccgcca
    35881 gccggtcatg gcatagtact tggagaagcc gttcaggacg aaggcgcgat cggtgtattc
    35941 gaggatggag tgggcttttc cggaatagac gagaccatgg taaatctcat cggagatcac
    36001 cgggggaccc agcgcggcga tgtccgccat ccggtcggca ccgagcaggt tcccggtcgg
    36061 attggagggg gagttgatca tgatggcccg cgtgcgcgtg ctcagctttt cgcggatgcc
    36121 ggcgggcgtg tattggaatc cctcctcttc ggacacgtcg acgctgacgg gacaggcatc
    36181 gaagaaccgg atgaagttcg ggtagcaggc gtagtggggg tcggaaatga tgacctcgtc
    36241 ttccttttcg agcagggcgg caaagatcag gagcatcgcg ggcgaggtgc cggaggtgac
    36301 gatgatctgg tccggggaga tgcggacgcg ataggtcagc cagtagtgtt tgcagatggc
    36361 ttcgcgaagt tcgagcaggc cgaggctgtg cgtgtagtgc gtcttgccct cccggacggc
    36421 gcggaaacag gcttccttga tgcactccgg ggtgtcgaag tccggctcgc cgacctccag
    36481 atggatgacg ctgtcgcccc gcttttcctt ttcctgtgcc ttctcgagga catccatgac
    36541 gatgaagggg cgaagggtcc gggcccgatc cgagatccgg ttgttgcaag acggggtggc
    36601 cgccgtttcg ttcatgacga tccgttcctg gagtgaagcc gtcctgcgga cggcggtttg
    36661 atcgggccgc gacgctggca cgggagagag ctgccagcgt gcggattacc ccccaaacgc
    36721 tatccgcacg ccggcttcct tgtcaaggga aaagggctgc cgccgtcagg accggttact
    36781 cccccggagg tatccggggg ggagggtgcg gcgaacgacc cggtctcgca tgatggcggg
    36841 ccgctcgaaa tcggcggcga tcttgagaaa ggtcatcgtg atgttgaagg tcgctcccca
    36901 gagaacttcc aggcggccat ccgcttcgcg atagaggaga cagggaaact cggcgggcat
    36961 tccggtatgg tcggaggtcc catcgggcag ggaaaaatcg aaccaggcgt aattggccgg
    37021 atcgaagaag gcgcggagcg ggatctccac gatccgttcc acctcggcat tgatccgggg
    37081 gcgccaagtt ccgtcgagaa ggccgacaac gggaaagatt cttcgcttaa gaagcagcag
    37141 gctatacggg ggaagggcgc cgagaaatcg aacctgccag ggccgcagac gaacctcttc
    37201 ccagctctcc cggagggcgt ttgccagaaa caggaggacg gcgcgacggg tagcggcgtc
    37261 ccggcgggcc agttcccggt tcacggcccg ggggatgatg gggaagaggc gggggaggag
    37321 gcatcgtccg accggatccc ggcgtggatc gaggatgccc ccgggacagc tgagatcgcc
    37381 cccctgggcc accgtggctg agcgtttgat caggtgcagg acgaattctc cgttgtccgg
    37441 gtccggcgag agcgggtcgg tccggaagga gaggagaaac aaaacgccgg ccggaagaac
    37501 agggtccgca tcgccggacg atgggggtag agcatagtcg agttcgcatc gacccaggca
    37561 tgagacgatc cggttcacga agcgttccct gtctgccagc aggcctcggt ccatcaggaa
    37621 cgccccccag ggtttcggcg ggagcgatga aacgcctctt caagttcgtc cagcatccgg
    37681 gccgcgatcc aaggttcgta ccagcatccc ggagggtaga gttgcatgaa tccgcaatgg
    37741 cctccgtagg gctggatgag gatctccgcg cgggcgttgt ttccgagcgg gtaaaaatct
    37801 tccacgggga cgaccgggtc atcccgcgag gtaatcatgg tcaccggtac cgatagaggc
    37861 accaggaggg cgtcggtgag ggtgtaagcc cgaaaatagt cggtgtagtt ctcgtattcc
    37921 ggattatgag acatcagggc ttccgtgagg ccgatgagcg ttgtatgcga aaggtgtgcg
    37981 gagaaatcgt aacaattcgg aaacagggcc tgttttctgc ggagggagcg tttccacttg
    38041 tggagaaaat agtggcgata cagggccggt ccccggtcga tggccagggt ggccttgtag
    38101 gggtcgagcg cagggctgat cgagaagatt ctccgcagcg tcggcaggga tccgttgccc
    38161 gcccggaggc caatgcgcaa ggcgaaatta cccccgaggg agaatccgac gatgaaaaag
    38221 ggcatcccgg gagagagggc ggcgacggcg atcacggcct ggaacacctc gtcgatccgg
    38281 gcgccgtgaa agagatcctt gttcaggtga tgactgtccc cgtggtcccg gaggttcagt
    38341 cggaagacgt cgaacccccg gcggaacagg gcacggccgc aagacagcat ataagctgaa
    38401 gcggcgcttc cctcccagcc gtggatcatc gccacgagcc cccgggtggg gggatcatga
    38461 cgtgaccaga acccctggag ccggaccccg ccgcccgcgt cgagaatgag ttcccttgct
    38521 gcggtcacca ggggattcgg tccgaggacc cgcagtttca ggctggcgag aatcgactgg
    38581 agatgagggt tgcggatcac cggtcccgga tcgaagtgat cgatcctgcg cttggaggaa
    38641 ccttctgcgg cgagggtgga cacgttcttg aagcggtccc gtttcatggg actgtcgata
    38701 caacacggta aaccgggtgt caatcctctt ctacgctatc gaacggtgag aagacctttt
    38761 cgggcattca accctcctgc acgtggaaca gaacgctccg atcgggtttg ctcatcgcca
    38821 atccccgact gcggcgatag caacgccaag agacccgcga gcgctcggaa tgcggttcag
    38881 aacgcggcgt ggagaaaaaa aaggacttag aaaagctcct aagtccttgt cgtcttggtg
    38941 gcggtgcagg gaatcgaacc ccggacacta cggatatgag ccgtatgctc taaccggctg
    39001 agctacaccg ccaagcgagg gccgagctat aacagatcga ccggaccctg tcaaccccct
    39061 attgctgcac cggaggcgcg taatgtgcgg acatcttgta ggccacgatc acgctccgtt
    39121 gaccgatcgt ggcgccgacg gactggacca aggccagaac gatttcgttt tgaccatcgt
    39181 tgtcgatgtc cttgaactgg tagtccgcga cgtaaccttt gatcttgcgc gtcttccagt
    39241 tttcgatcaa ccccagtccg tcccattcga gattatagac ctcgctcgac gtgaacaact
    39301 tgacattttt caagatccgt ccggttgagg ataggttctt gacgagaatg atttccggtt
    39361 tcttgtcccc gttggtgtcg tagatgagaa tccgcggatt gatgaaatac cggtcccgtc
    39421 cctcgtcctg ataggcgctc ggttggatgg aagacaggtc gatggaaacg ttgctgccgc
    39481 cgaacacctc gtcgctcttg aagagacgct ccttgcttcc cccgaacacg gcaagtttga
    39541 ccaacggctt ggacgtctgt tcgtagatgc acaggtagtc ggactcgtcg atggcgatga
    39601 tcttttcatc cgagccgagc cccagatcgt cgagggcgag tccgtagacg gacaggccct
    39661 gggggatctt catccgctgt ccctcacggt atcggtcgtt ttcccagacg attttatgaa
    39721 tcggcgtgtc gaagggatgt tccggggaga tctcctgtcc cagcaggcgt gccgaatcgg
    39781 aggacgtgcc gatcacccgg agaaaccagg ggagatcgga ggcgatcggc gtgaatttgc
    39841 cttccctgaa ttcgatcacg aaggacgcaa gccgggtctg ccggaggcag gtgacgatga
    39901 tttcgtttac accgttgcgg ttgatatccg cgatatcgac ggcgagatac ctgtcgtagc
    39961 gcttcccgga aatcttctgc agaagcttga aaccggcgtc cgtcttctgg tggatgtaga
    40021 cgttgtagtt gtcgatgaga accacttcgt tcaggcggtc gccgttgacg tcgccgatat
    40081 ccattcccct gaattccgtc ggggaagtcg ggctcatcca gaagcccctt cggtcgaggg
    40141 gttgggcggc gttgatgaaa tccggattga tcgcggcggt gaacgttccc tttttactcg
    40201 tgcgcatgcc cgcgatgatg gccgcttccc gggcatcgcc accggccggg ggagccgcaa
    40261 ctctggccgg cgcctccggc gggggcgcaa cggcgggctg gggttccgga gtgacgccgg
    40321 tgatgtgcgt attgatgcgc tgcgcaaaat cggagatccg gggaatgacc tcatccaggt
    40381 tttgactctg gacgaagatg cccacgggtg acttgtaggc cgcgacatcg accagtttgc
    40441 cgtcaatgct caggctgttc ccgatcttcg tgatgctgcc ccagacgacg tagtcggtct
    40501 tcatcgtttt gccgagttcg tagacatcgg cttgggaaag ctccttgccg gcaaaattcc
    40561 cgagggcgtc ctgaatctgg tcgtggctcg ggacggcaag gcggccatcg gacgaaaggc
    40621 gggaggcgag catctcccag atgccctgct tgatgtaatc cagggattcc gcgctgtgga
    40681 cgacgaacgg caggacggcg acggatttct gctccgcggc cccgatgagg gcgccgggga
    40741 tcaacaggat cagaccaatc aagacggaag agaaaagttt cttcatcatg gttcaccttc
    40801 ggaatgcggg gtctcgggat cggcatggtg cgctcgcccg gccgcaggat atgtagcatt
    40861 gaaagggtct ttgttcaaga aacatctgcg gccctccgtt tacgcctctt cggaaaaggg
    40921 gatggtcagg ttgtagatgc cgaactccat aacggcgcgg atggggtagg ggagcttctc
    40981 gtaaaccttg agcatcggct tgttgtctgc gagcacgtcg gccgtgatcc cctgaatgcc
    41041 cttttcctgg gcgtatttaa tgagcaattc cagcaggaac gtcgcgatac cgagcccctg
    41101 gtacttctcg tcgacgatga aggccgtgtc ggcgtaggga cggtcctgat ggcggacgta
    41161 ccggccctcg gcgatgatac gctccagccc gtcctcctcg atcaggccca caatggccat
    41221 aaaccggcga aaatcgacgt tgacgtactc ctgcatcttt ccgtgcggca tggttttgat
    41281 ggaggtgaaa tagcggtaat agaccatgtt gtctgagaat cggtaaaaca ggcggcgcat
    41341 ctcctcctcg tcggagggct tgatcgcccg gaaccggacg gtcaggtcac cgcggaaggt
    41401 atgcgtgcag gcga
//
LOCUS       DAOOLJ010000017        35756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_944_length_35756_cov_18.178706, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000017 DAOOLJ010000000
VERSION     DAOOLJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 35756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35756
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_944_length_35756_cov_18.178706"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1828
                     /locus_tag="PLW16_04905"
     CDS             62..1828
                     /locus_tag="PLW16_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB70278.1"
                     /translation="MKAAQKVVWEDRPARYRRPLIQAIAVALVSIIFIGLILIMGIRD
                     LKTIDRTLMGFLENRGQGVINVVERLAQENINTLVQGQQQRHDDTFVPLAEEAFSPQR
                     LLIRALVNIGREVDTNWKADRLSENTLRKMVAEQGLWMIAVFDKGGRAVFQSRSLPSS
                     LAGSQGADDSVAIEDFSRLGRDGKIGFIALRRRDGSGTIIIALDPTGARYWGLKVAIE
                     KAIGELGEGQRQNLLYLVVMDRQGKVLGSTGQGPQSWGSGEFRLPELLDGRRKIESRK
                     GVWQGKNILDMAIPVTLNNRIEGVARIGLDRSGSDRILRENERNMLVFMAFIVAIMLL
                     AMGLLYFNQNRHMERLVEITRQLEKAERLSALGQLAAGVAHEIRNPLNAISMASQRLK
                     REYLPDNPDKVGDFGILTGVIRDEIRRLNGIIEEFLTFSKSRRLELREYSVVEVLQKI
                     VNLIREEAVTKGIALETHWGEDPLVIPMDVDKLQQAMLNFIKNAMESISGAGTVMISA
                     ALQSRERLIIAVSDTGCGMTPEEVERIFNPEYTTKEKGLGLGMTLAHEIIRGHGGEIR
                     VKSEAGAGTTVEIILPTESRKS"
     gene            1856..>3047
                     /locus_tag="PLW16_04910"
     CDS             1856..>3047
                     /locus_tag="PLW16_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB70279.1"
                     /translation="MKALNILVVEDGQSQREMLRDFLLREGHRVTEAETGPGAVEAVH
                     RGQVDLILLDYKMPGMDGMEVLKEVKRLNPEIDVVIITAYGTIETAVDAMKAGAIDYI
                     TKPVEFDELLLLIDRVCERRILIRENEILRQNLREKGVTAEQIVYASEKMKTLINLAG
                     RVAGSRATVLLQGESGTGKELFARLLHHLSPRSGRPIIAVNCGALPETLLESELFGHE
                     KGAFTGAGARRIGRFEEADGGTLFIDEVGELYPSVQVKLLRFLQEREFQRLGGNQTIP
                     ADVRIIAATNRDLEARVREGAFREDLFYRLNVVVLSIPPLRERKEDIPFLIDHFLQRF
                     ATENGKRIEGISREARDLLLKYAYPGNVRELENIIERAVVISRESVISVEDLPFGGGA
                     PEVRE"
     assembly_gap    3048..3147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3185..3313
                     /locus_tag="PLW16_04915"
     CDS             3185..3313
                     /locus_tag="PLW16_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB70280.1"
                     /translation="MERRLILEAMEKAGDHQTRAAEILGISERMLRYKLKKYGLKS"
     gene            complement(3419..>4877)
                     /locus_tag="PLW16_04920"
     CDS             complement(3419..>4877)
                     /locus_tag="PLW16_04920"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB70281.1"
                     /translation="AHRTRPAPDPGEYLEPLAGLMALDRGAGGGTGGRRGRMKRGRLN
                     QDHVFYRNPARFYPAVDRGRGVYLYDTDGKRYLDGAGGAAVTAIGHGVKDIERAVVRQ
                     LRKVAFTHGSQFTSEAAVTLAGRLVALAPAGLNRAYFVSGGSEAVETALKLSRQYQVD
                     RGQPSRFKVISRWTSYHGNTLGSLALSGHTGRRRDYLPLLPHTPHIAPAYCYRCPFGL
                     APASCTLECATDLEKTILHEGPESVSAFIAEPVVGATAGALVPRDGYFRRIREICDRY
                     DILFIADEVMTGLGRTGKAFAVDHWGVKPDLIACAKGLGAGYSPIFAVLATEAIHETI
                     RTGRGAFVHGHTYGQNPLACAAALAVLEYIEARDLIARSAALGPYLLDSLARLNRHRI
                     VGDIRGLGLFAGVEFVRDRASREPFPPEARIHARIAERAFARGLIVYSGGGGADGIRG
                     DHVLIAPPFVITKRQINALVRLLDKAIADIAGETG"
     assembly_gap    4878..4977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5089..5565)
                     /locus_tag="PLW16_04925"
     CDS             complement(5089..5565)
                     /locus_tag="PLW16_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB70282.1"
                     /translation="MSFSASDILEFAMRIEENGANFYRYAVQITKDEEAKEIFARLAD
                     EELNHQKTFGRIFAAMEKTAPPESYAGEYAAYLRNYVDNTVIFTKEALDRELAGVKDT
                     LSALDFALRREQDSILYYHEIKQVVPAAEHAAVDRVIEEERRHFGILSEIKKRFQK"
     gene            complement(5697..6767)
                     /gene="corA"
                     /locus_tag="PLW16_04930"
     CDS             complement(5697..6767)
                     /gene="corA"
                     /locus_tag="PLW16_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPB70283.1"
                     /translation="MSSPQPTRVGKVGLPPGSLVHVGRRLTEKTCLTLFNFDEQHIEE
                     RTIAAVGDAVPYRNRPSVTWLNIDGLHDTGVLAALGEAFGLHPLVLEDMLNTEQRPKF
                     EDLGDYLFIVMRVFMLPANPEGGIRSEQISLVIGPHFLITLQEKAGPLLDPIREGLRA
                     GKGRIRKGGADYLAHAIVDAVVDQYFVVLEALGERIEVLEDRLMTRPTPDTLRAIQGL
                     KREITSLRRSVWPLREAISAVDRSDSTLLHEGTGIYFRDVYDHTIQMIDTIETFRDQL
                     AGMLDIYLSSLSNRMNEVMRVLTIIATLFMPLTFIVGIYGMNFKYMPELAWRWGYLYV
                     WGVILLAVAAMLLYFRRKRWLP"
     gene            6942..8348
                     /locus_tag="PLW16_04935"
     CDS             6942..8348
                     /locus_tag="PLW16_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB70284.1"
                     /translation="MKGILVVVPDRNVVQVVRGIFRSRGTVDAADDLDACLGLCRKKR
                     YELALIDLALLQALSSRHGQYDYKSSLQQLWQIQPALEIIVMAGQDAVREAVMAVKAG
                     ASNYVTYPLNRDEVRYAVELIAEENRTRSELDYLRSRFWQADALEVVQTKNAFMRSVF
                     EAIRLVAPTKSTVLLTGETGTGKGILAKLIHCHSNRKDVQFIGVHCGAIPDTLLESEL
                     FGHEKGAFTGAVRRKLGKFELAHGGTIFLDEIGTLSPAAQIRLLQILQDRTYERVGGE
                     ETLEADVRIVAASNVDLKKLVEEGLFRQDLYYRLNVFPIELPPLRERTEDIPQLVEVF
                     LRNLNRFNQKDIHYAHPQVMDAFRRYPWPGNIRELENLVERAYILETSSMLTPESFPA
                     ELFTVTPTPPRLSLNTSLTLGEVRRQAVEAVERQYLKELLSVNRGRIDRTAVAAGVTG
                     RQIHKLLQRHEIRKEDFK"
     gene            complement(8341..9792)
                     /locus_tag="PLW16_04940"
     CDS             complement(8341..9792)
                     /locus_tag="PLW16_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPB70285.1"
                     /translation="MDEMATLFQNFRLQDLFDIVIIAGMIAILLIWFKDRASRFVFSG
                     VVLLGFLYGAARFSHLYLTTVVLQGFFGILAFALVVLFQEDLRRFLERLALWGRFRKQ
                     WREPVAHEETAEILALTAANLARNHIGALIVLTGSDPLDRHLSGGNRLNGLLSQPLLE
                     SLFDPHSAGHDGAVIIDGNRVVRFGCHLPLSTGAGKNQPMGLRHTAALGLAERSDAVC
                     IVVSEERGTISLARGEQIQVLPHAAALKSALSAFYAARMPVRAAHPFWQRLKTNPREK
                     VIAILLACVLWLVFGYQRDSVRRDFLAPVEYVNIAADWVIENPKIPEATIMVMGPSQA
                     FQFLDVGSLKISLDLSRLREGRQEIPLTRNMVRVPANLTVLDIKPNHLVLSASRLVPV
                     SVPVRVATEHHPPPGYAVGRITVSPETVRILVPSKRRTDKIAIKTAPVNLQRLRETRT
                     VEVKLTPPSDVQLAPDTAATVRVTVEIVGQDAT"
     gene            9907..10215
                     /locus_tag="PLW16_04945"
     CDS             9907..10215
                     /locus_tag="PLW16_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70286.1"
                     /translation="MKKIAMCVLLFGIALLSLVVPAPGGETISLSGMVFVVDWDEEDR
                     ITAVSVVTEEGREYRVLPDAVGRELFQREGDTVDIEGVAGTDAAGRKTLTVIRYRIRP
                     "
     gene            10303..11082
                     /locus_tag="PLW16_04950"
     CDS             10303..11082
                     /locus_tag="PLW16_04950"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPB70287.1"
                     /translation="MADIHLDEKIGAVAGMIVAAERVVVFTGAGVSTESGIPDFRSPG
                     GIWSRFDPEDFTIERFLVSAETRRKQWEILLDGGLLTDARPNKAHLAIAELEALGKLT
                     CVITQNIDQLHQKAGNRPEKVFELHGNMQWLRCLGCDTRYPLDEMLSRYRSHPEVPVC
                     ERCRGILKPDAVFFGEALPEAALGAASYHARHCDLFLVVGSSLVVYPAAYMPVYAKGA
                     GAKLAIINLSATLCDDQADILINATAGDVMERILRDVRARL"
     gene            11109..11873
                     /locus_tag="PLW16_04955"
     CDS             11109..11873
                     /locus_tag="PLW16_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPB70288.1"
                     /translation="MCLILFAYGMHPRHRLILAANRDEFYARPSAAAAFWADAPDLLA
                     GRDLRDGGTWLGITRQGRFAALTNFRDPASLKTGAPSRGRLVGDFLRGQQAPPDYLDA
                     IRAGASAYNGFNLLVGDFRSLYVFSNRGGSMKLSPGLYGLSNHLLNTPWPKVERGRAF
                     LRALLENGGDPETEALFSLLADRSRPADANLPDTGVGPAWERILSSIFITSPVYGTRS
                     STVLKIGHKGGVEFVERVFNGRPEAWMTSAFSFSAL"
     gene            11933..12688
                     /locus_tag="PLW16_04960"
     CDS             11933..12688
                     /locus_tag="PLW16_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HPB70289.1"
                     /translation="MKVIFHEDFYEVYAGDPAAAAGRMEAVVKAIEGRVTFVEAVPAA
                     EEDIAAVHTRSHIEQVRREGVYTVAALAAGGAIQAARTGLKEPCFGLIRPPGHHASAG
                     SAWGFCYFSNMAIALERLRRDGLIKRAFVLDIDLHYGDGTVNVLEGRGYVRIYNPSTN
                     DRNDYLKRVRDKLRAEPVDIIGVSAGFDHHREDWGGLLTTEDYREIGRMVKEAGEAHG
                     AGRFGILEGGYNHRVLGRNVLAFLQGLEGGEEN"
     gene            complement(12689..13753)
                     /locus_tag="PLW16_04965"
     CDS             complement(12689..13753)
                     /locus_tag="PLW16_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB70290.1"
                     /translation="MSGTRTRRSPSPARLVPILLLLFTTGCATQIRVNMLQPAAYHEA
                     SLTKVVAVLPFDGPGGAEFAAEMEGVLNSINIDDKPYFTLVDRASIDKTVSELKLSQS
                     GLIDPKTAVKIGRLVGAQGIYTGVVGAYGWQDTPFKAKRSECVKREVRRGKDGRLYEG
                     ACIRWRTFYVNCIKRTASISGTPKLVEVETGRVLYARNLSGAAESSGCEDTRPAADGE
                     ALFTRAKETAQAQFRRDIAPYYVTREITLMDTGDGITAPEAKEKLKQGIAYAGKGRLD
                     MACELWGAARILSPDAVAIQYNLGVCAESRGDFEAALRLYRQADRLLGKPDDNITLAL
                     NRAGQALQNQKKLKEQLQEE"
     gene            complement(13764..14942)
                     /locus_tag="PLW16_04970"
     CDS             complement(13764..14942)
                     /locus_tag="PLW16_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70291.1"
                     /translation="MRKTLVCLAVGLCVLVFLAAPGFGQEKCVDAEGEAIVIGNDLPS
                     ARLEAIARAKWAAIERTVGTEVKAQSFVQNFTLVDDAIKTQTGGAVRRFKVLRQETRD
                     DVVVVKINACVEPTRARDALSALALNSAVAVFIPARVPNTRENEDAFEETNILSETLI
                     GRLTEQGYSVVDVAPTGAADAAAIEKAAKGGSSVLLRSLMYRFLSNLIIIGKLDYNIS
                     VKKGEDIGYGIVMPFQNVTVRLTYRMVARNNKTGKMEILTAGVEQARGLANSVEDAAA
                     RAMEDMAEVLTPKLLDKVAEYIQGNVKKVRIEVQGVKTLDANMEVQDLLQTLVWVTNV
                     EEKGMGQFVVSYPENTLYLANSLNQKEGVDVVNFSPYSLTLKYTGEPSRSDRRPPDEE
                     "
     gene            complement(15029..15367)
                     /locus_tag="PLW16_04975"
     CDS             complement(15029..15367)
                     /locus_tag="PLW16_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70292.1"
                     /translation="MNATRRHVHQPPGTEVRAPWGEYTVRREGRIPYDDRDVLYVLAD
                     IAVGSACRGTATGRILIVPGYIGTGGDGPREENPARSRITPVRTARDRGAITRILRAR
                     VATAAVVFPP"
     gene            complement(15364..15690)
                     /locus_tag="PLW16_04980"
     CDS             complement(15364..15690)
                     /locus_tag="PLW16_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70293.1"
                     /translation="MADYTHREVGEEVHFIAGHYEIIEEGRLTHAGREFLYALGIATL
                     DNACCGRSGCRFLFIPGYVRSWKVRTNDQGMPVSDVEPITDEREQAAIRAFLDARYPY
                     SQINFS"
     gene            complement(15751..17130)
                     /gene="mnmE"
                     /locus_tag="PLW16_04985"
     CDS             complement(15751..17130)
                     /gene="mnmE"
                     /locus_tag="PLW16_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPB70294.1"
                     /translation="MNIHDTIAAVATPPGRGGVGIVRISGTEAEAIARHLFRTDGPLT
                     SLASHRLYHGRIHSPETGEPIDEVLLVLMKAPRSYTGEDVVEIHGHGGPVLLRTLLAE
                     ILGAGARPAEPGEFTRRAFLNGRMDLAQAEAVADLIDAHSAPAATLALGHLEGRLSRA
                     VEGLYARTTDMLVLLEAAIDFGEEGETALDAGALAARFGEILEDLRTLLGTYAAGRVR
                     REGFRVVITGRPNVGKSSLLNRLLGEERAIVTPFPGTTRDFIEETLLIEGIPFRLTDT
                     AGIRTPEDAIEQAGVAMVRRRLSHADAILLLLDGSMPLTPEDEALLGEMHGRPTVIAV
                     NKADLPHVLAEDRLHAFRPLHPPLWISAKTGEGIDALKALLYAASCGGEPATRDGALI
                     GNLRHKLALEAAKTHLERGGEALERRVSPEFIALDVRDALQSLGEITGRTASPEILDK
                     IFAGFCIGK"
     gene            complement(17117..18061)
                     /gene="jag"
                     /locus_tag="PLW16_04990"
     CDS             complement(17117..18061)
                     /gene="jag"
                     /locus_tag="PLW16_04990"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPB70295.1"
                     /translation="MKSIEIEGKSIDEAIVKACEAFAVPREKLQIEILSEGAAGFLGI
                     GARKARIRAGLMNLSLDLDDREDATAEPVPDVPAPERTSLFKQEPGPAPQPVGEAPPP
                     PGRRREPAARPAPETPPRPKRSPAPPHRGAEKPPVESRAAAATPDPEPAGDVALQAKA
                     VLEGILERMDLKYPVSVQETEDAIRLDIQGDGGGLLIGKRGQNLDALQYIVNKAAYKA
                     GNGKKVIIVDTETYRQRREESLVSLAERLGEKVRKTQKPLTVSHMNAHDRRIIHLALQ
                     NDTQLATKSRGEGEYRKIIIMPARSRPPRDQADPHEHS"
     gene            complement(18076..19767)
                     /gene="yidC"
                     /locus_tag="PLW16_04995"
     CDS             complement(18076..19767)
                     /gene="yidC"
                     /locus_tag="PLW16_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPB70296.1"
                     /translation="MDKRTFLAIVLSLAVIFVYQAFFVKPVPQRKPAATPPPQTGLTQ
                     PDRTASAPPPSPAATSAPPVAPLPAVRAAAVAERDVTVETPLYTAVLTTRGGALKSFQ
                     LKGYRTELPEDRRYGFDGLLALVGFKKKEEKPALPVELVHVQEGMPRPLTVSFPDSSL
                     PLPAEGGFTTGTTALDLTTGTEGRPLVFTQILPGTARVEKIYTFRPDRYVVDLEVRVR
                     NLSGNPLNQHAALNWRRYVDPKAETDRYGNEGPVTYVAKSIDRIEVKKIEREKIHGPD
                     VSWGGFESKYFLAAMIPQNPSLSSVAIDRDTRDMVTVSLRGANTIIPAGQSGSFVYSV
                     YLGPKDYTILEAQGVGLENAIDFGSWLKWLAFPLLIVLKFLYQYIPNYGIAIIILTAL
                     IKLVFWPLGNKSYQSMKEMQKLQPKLKALQEKYKDDKAKISQETMALYKAHKVNPLGG
                     CLPMIIQIPVFFGLYKALLYAIELRHSPFFWWIQDLSARDPYFITPLIMGATMFIQQK
                     MSPAPGDPMQAKLMLWMPVIFTFLFLYFPSGLVIYWLFNNLFSIGQQYYINQRST"
     gene            complement(19795..20019)
                     /gene="yidD"
                     /locus_tag="PLW16_05000"
     CDS             complement(19795..20019)
                     /gene="yidD"
                     /locus_tag="PLW16_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPB70297.1"
                     /translation="MLETIIGKMFIGLIRLYQLFLSPLFAPCCRFYPSCSEYALTAIR
                     RHGPLRGLGLALWRLVRCHPLHPGGYDPVK"
     gene            complement(20012..20371)
                     /gene="rnpA"
                     /locus_tag="PLW16_05005"
     CDS             complement(20012..20371)
                     /gene="rnpA"
                     /locus_tag="PLW16_05005"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPB70298.1"
                     /translation="MERKTFAPHERIHKRRDYLKVYEQGTREHTPNFTIITLESPSGT
                     RRLGITVTKKVGNAVKRNRIKRLLREYFRLNKSSLPPSRDMVIMAKKGIPWMTYRDVC
                     RELGRLRFAPADHETHA"
     gene            complement(20426..20563)
                     /gene="rpmH"
                     /locus_tag="PLW16_05010"
     CDS             complement(20426..20563)
                     /gene="rpmH"
                     /locus_tag="PLW16_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPB70299.1"
                     /translation="MKKNLTAVTNVKRKRTHGFLVRMATKGGRRVINRRRAKGRKRLT
                     V"
     gene            21008..21283
                     /locus_tag="PLW16_05015"
     CDS             21008..21283
                     /locus_tag="PLW16_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70300.1"
                     /translation="MKKFMQTLWVTLFVLLTTATFGYAEFTAAGASDFPYFHLGCLIV
                     GGLIIVSLKHKYNRIYLSEALGSFALYTVMVALFTAPVVDALKTLVS"
     gene            21520..23523
                     /gene="uvrB"
                     /locus_tag="PLW16_05020"
     CDS             21520..23523
                     /gene="uvrB"
                     /locus_tag="PLW16_05020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPB70301.1"
                     /translation="MDAFRMMTDFTPQGDQPRAIDALTAGLASGRPHQVLLGVTGSGK
                     TFTIAKVIEAAGRPALIIAHNKTLAAQLYQEFRALFPDNAVEYFVSYYDYYQPEAYIP
                     STDTYIEKDSSINDEIDKLRHAATHSLLERRDVIIVASVSCIYGLGSPEAYAGMLLLL
                     EGGMEISRDDVLKRLVEIQYERNDIDFHRGTFRVRGDSVEIFPPYEEERAYRVEFFGD
                     TIDGIALVDPLRGKKLGPLSRLAVYPGSHYVTTRDNLVRAVGDIRAELAERLAAFQAE
                     NKLLEAQRLEQRTHFDIEMMEELGYCQGIENYSRHLTGRRPGQPPPTLMEYLPKDALV
                     VIDESHATVPQLIGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEGFPQQRIYCS
                     ATPAAYELQAAEDRVVEQIIRPTGLMDPEIDVRPVTGQVEDLYAEVRARAEAGERVLV
                     TTLTKRMAENLTSYYEELGVRVRYLHSDIKTLERVGIIRDLRLGEYDCLVGVNLLREG
                     LDIPEVSLVAILDADKEGFLRSERSLIQTSGRAARNVAGRVIMYADRVTPAIRACLDE
                     TRRRRSLQERFNRKHGITPATIRKSIHDVLGSIYEADYVTVPTVAEKRKAYGTEEEIA
                     ALIRDLTAAMKASAAELAFEKAAALRDEIRELKGLLVELGGKA"
     gene            23520..25403
                     /gene="uvrC"
                     /locus_tag="PLW16_05025"
     CDS             23520..25403
                     /gene="uvrC"
                     /locus_tag="PLW16_05025"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPB70302.1"
                     /translation="MTAGAASPDVLKAKVALAPRAPGVYLMKDGEGRILYVGKAGDLK
                     SRLKAYAGGTDGRAMIPFLVSRVRDVELILTASEKEALILENTLIKAHRPRYNVDLRD
                     DKAYVHLRIDPATPFPRFQVVRRPKKDGARYFGPYPSASAARETLRFLQSVFPLRTCR
                     DAELKSRRRPCLEFEIRRCLAPCVGRVGAEAYDRLVRESCAFLEGGGRDLTDAIRRRM
                     QAASEALDFEEAAALRDRIAAIEETLARQRMEDVTEADQDLFGLHREGDDLQVCLLTV
                     RFGKLSGQRLFPLIRLAGETAELLSSLLKQFYDAGAHVPGEILLPLDLEDAAAVAEWL
                     AEKKGRAVSLRIPMRGKARDLLEMARRNAENALRTKRLAEFDPAEALEILARTAGLDR
                     PPRRIEGFDISNLGGRHAVGSMVVFVDGRPEKADYRRFRVRTVPGADDYAMMYEVLKR
                     RFSRALAGAPGDGARPFPPLPDLLLVDGGKGQLQVAVTVLKELDLKTVDCLALAKERQ
                     EAVRMPGTVDRGEDRVYLPRRREPLYLSRRPAALFLLQQVRDEAHRFAVAYHRRLKTR
                     DDFRSALDAVGGIGPKRRRDLLAFFGSARRIAAASLEDLQKVAGVGRQTALRLRRHFN
                     PEG"
     gene            complement(25661..26062)
                     /locus_tag="PLW16_05030"
     CDS             complement(25661..26062)
                     /locus_tag="PLW16_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HPB70303.1"
                     /translation="MKKILLMISILSLAGCATGPVLYPNSYLREVGEAKAHEDIAECE
                     TLADQYVQSDAGIQAAKDMAISAGGGAIIGGAAGAVVGHLGRGVGVGAATGAAAALVR
                     GVIRASEPSPLFKSFMDRCLQERGYEVIGWQ"
     gene            26310..26870
                     /locus_tag="PLW16_05035"
     CDS             26310..26870
                     /locus_tag="PLW16_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPB70304.1"
                     /translation="MGFGAILIIAVGLGMDALSVAVGIGGARRERTWGPTLRLALSFG
                     LFQFFMPVAGWLAGRTVADIIAAYDHWIAFALLAYVGGRMIRESFRGGEASPPADPTR
                     GWTLLMLSVATSIDALAVGLSLAFLKTPILFPSAVIGAVCFLMTVAGMRFGERLGRLV
                     GRRMEAVGGLVLIGIGLKILIEHLAG"
     gene            complement(26969..28099)
                     /gene="proB"
                     /locus_tag="PLW16_05040"
     CDS             complement(26969..28099)
                     /gene="proB"
                     /locus_tag="PLW16_05040"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPB70305.1"
                     /translation="MNPIRQTVLAPVRRVLIKVGSAVLTGADGLDLTIVQQLVDDIAL
                     LTKSGRQVVLVTSGAIASGKHRMGITGELKSMPQKQAAAAIGQGRLMRVYSNAFGRHG
                     LYVAQLLLTLSDLTDRKRFLNIRNTLSTLMEWGVIAVINENDSVAVDEIKFGDNDHLA
                     AMIANIIEAHLLVNLTSTEGLYDRNPGASREARLIPLVREFTEDILEAATAETSDVGT
                     GGMRSKILAAQKVTAFGIPYIIAPGKRPGVLGALFAAEAVGTLFLPMQKHLTSRKHWI
                     AFTLRGKGKLVIDDGAKWALLEQGKSLLPSGVVDVEGDFGIGDPVVCVDACGAPVAKG
                     LVNYAADEIRKIMGLKTARIAQVLGHKDYDEIIHRDNLAIRK"
     gene            complement(28096..29124)
                     /gene="obgE"
                     /locus_tag="PLW16_05045"
     CDS             complement(28096..29124)
                     /gene="obgE"
                     /locus_tag="PLW16_05045"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPB70306.1"
                     /translation="MKFIDEAKIYVKGGDGGRGCVSFRREKYVPRGGPNGGDGGKGGD
                     IILVASGSHQTLLDLKYRQHHIAKHGGHGEGSDRTGRNSEDIRIPVPAGTVVKDAESG
                     EVLADLVRAGETFIVARGGIGGRGNARFATPTNQAPRYAQPGMPGEERWILLELKLLA
                     DIGIIGLPNAGKSTFISRVSAARPKIADYPFTTLTPNLGVVKVGDRAPFVVADIPGLI
                     PGAHRGLGMGIKFLRHVERTSVLLHLIDLSGDAAPAGETMATINAELRHFSPGLGARP
                     QVVAISKIDLTDTRERLEAETTALLRQRIRAFPFSAVTGEGLPALLHALADALDAARE
                     KNTEEVTP"
     gene            complement(29128..31695)
                     /locus_tag="PLW16_05050"
     CDS             complement(29128..31695)
                     /locus_tag="PLW16_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB70307.1"
                     /translation="MGFRIRLDRFFTKNKGLYYKLSLIFALFFVAPVLGFLYFAVKYD
                     ILGDRYLPLFFIVLLIAFFLGFVILRRVFDQILNLSRDISQSLAPELAGNRATTTDEL
                     NGIFHSFRTLEGELRKNLGHLEKKAAEISTLKELSELCYITFNPDDLLYITLERALKL
                     ARADIGSVLILSPQRDAFHIQASIGLGEHVNMDDRIPFETSIAKYAVINKSPLLVENI
                     ETDTRFGRSSRSQYATRSFICMPLKSLQEVIGVLSLSRKREDIPFTQADVDILTPLVS
                     SASFTFDNLRLLAAGRERDRAMESTELFARTVNSTLRGTELFHTLLQELRRHIPYDIA
                     VLLDWDRSAPGKVSVLDVLAFVPTALTRGAAFDGEGSMLDRVIRQQSALFLDDLSALA
                     PPLEKALCPQGTNGTCLLAPLKVEGRITGVLLLYNVTRDAVDKRGRLLSMMTEGLSLA
                     VEKENVLASIMRRNQELATLRQIGSALSSSTFEMDKVLNYTMDMIRVTMNVEAGSLLL
                     LDGNELKFKVAFNLDEAPLKHFRIKLGQGIAGYVAARGETITAPDIRNSPHFSPEMDA
                     RTGFRTRSVLCVPMISQGQVIGVIEVLNKIGGVFDNSDIHLLQSIATSVSIAMENARL
                     YRETLSMAEAERGIRSLFQKFVPREVVDKILLRSDAEQPLVEEFRSLTLLNIDIRSFS
                     PLAKKLGPQKTVAMLNHFFAQMGEIVFRNHGIVDKYLGDGFLALFGAPVSSARDADNA
                     VAAALEMQETLPLLNAYFKEQFGTTLTMGISIHTGEVVVGNIGFEKKMDYTVIGDAVN
                     FVFKLQSLCKSWPDNILISEKACSSAQSSLNAEEIGVFEIEPTVAAQKIYRLLGIRK"
     gene            complement(31855..32115)
                     /gene="rpmA"
                     /locus_tag="PLW16_05055"
     CDS             complement(31855..32115)
                     /gene="rpmA"
                     /locus_tag="PLW16_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPB70308.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRRGVKVFGGQSIRAGGIIIRQLGTR
                     IHPGVNVGLGKDYTIFAKIDGVVKFERLDKRRKKVSVYAATA"
     gene            complement(32128..32439)
                     /gene="rplU"
                     /locus_tag="PLW16_05060"
     CDS             complement(32128..32439)
                     /gene="rplU"
                     /locus_tag="PLW16_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPB70309.1"
                     /translation="MYAVIKTGGKQHRVVEGEVLRVEKLEGSQGDTVVFEDVLLVARD
                     EDIRVGAPRVAGARVTGEIVSQMKGPKIAVFKKKRRKGYHKKTGHRQQLTEMRIKEIS
                     F"
     gene            complement(32499..35018)
                     /locus_tag="PLW16_05065"
     CDS             complement(32499..35018)
                     /locus_tag="PLW16_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPB70310.1"
                     /translation="MREEAFLTRVEKPGRYLGGEVNAVVKDHAACRLSIALAFPDVYE
                     VGMSHLGLQILYGILNAEPGVVAERCYAPWPDLEGELVRRALPLVTLESRTPLHAFDL
                     VGFSLQYELSYTNVLAMLDLGGIPLRAAARGEDHPLVIAGGPCAFNPLPMSPFIDAFV
                     IGEGEAVIGEIARALMAAKSRGERRASRLARLAEIEGVYCPALHTHGEIIRKRTVPDL
                     NAWHGPVCPVVPLVKTIHDRLVLEIARGCTRGCRFCQAGMLWRPVRERDPDILLQMSD
                     RMLAATGHEELSLLSLSAGDYPHIEALLGLLMDRHYARRVALALPSLRVETLTPRLME
                     VIRRVRKTSFTLAPEAGTQRLRDRINKGNTEADLMATAGNVFDAGWRSLKLYFMIGLP
                     GEGETDLDGIVDLAHKTLPRGKSPGQVAVSLSTFVPKPHTPFQWERQIDLAETEARQA
                     YFRRRLRHPRLTVRWHDGRMSLLEGLLSRGDERMGDLVERAYILGCRFDGWGDRFRFD
                     LWEAALDGLGVDREAALAARPPAAALPWDRIATGVSADFLREEAARALDGRLTEDCRT
                     GPCHRCGVCDHETIRIVPAGPPPETAAPSQPGTPAPSRPPVYRRYRLRFVKEGRARFL
                     SHLELAAILIRAIRLAGLDFRYSEGFHPHPRLSFAVATSVGMESREEYADIELIDPGL
                     DPERLRDAINRRLPAGIAVTDAAPIDPATPSLADAIGGFRYEITLPDDLDETDGRAIP
                     ERVSAFLAADTCPFVREGKGKTQTRDIRPYVADLKWAPEARRLTASVRFDARGAVRPS
                     ELLIHVLKLPEATAREARIVKTAVLGKEGGRESARPAQDLC"
     gene            complement(35015..35491)
                     /locus_tag="PLW16_05070"
     CDS             complement(35015..35491)
                     /locus_tag="PLW16_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70311.1"
                     /translation="MVKTTPRDGEWERRGGKTAGGGVAVPDGGIMKTLVETTVLDNGL
                     TMEVWDRSRPIAADTTKVELYVRVPVRLERDFFPGDEAFDVVRRIWDDPIVYEYVRER
                     TFVGNDAREAVFAELLAAFRAHTLPYLGRPDFARGLALARYREYLNNPHTFRNRLQ"
BASE COUNT         5947 a        11173 c        12140 g         6296 t
ORIGIN      
        1 gactttttgc ggtatgcaaa atcccgtgtg ttcagaatga cttggcctgg gaggtccgag
       61 gatgaaagct gcgcagaagg tggtctggga ggaccggccc gcccgctacc gccgtccgct
      121 gatccaggcg atcgcggttg ccctggtctc catcattttt atcggtttga tcctgatcat
      181 ggggatcagg gacttgaaga ccatcgaccg gaccctgatg ggctttctcg agaaccgggg
      241 gcagggcgtt atcaatgtcg tcgagcggct ggcgcaggag aacatcaaca ccctggtcca
      301 ggggcagcag cagcggcacg acgacacctt tgtgcccctc gccgaggagg ccttttcccc
      361 ccagaggctg ctgatcaggg ccctggtcaa catcggccgg gaggtggaca cgaactggaa
      421 ggccgatcgg ctgagcgaga acaccctgcg caagatggtg gccgagcagg gcctgtggat
      481 gatcgccgtc ttcgacaagg gggggcgcgc cgtctttcag agccgctccc tgccgtcctc
      541 cctggcgggg tcccaggggg ctgatgacag cgtcgccatc gaggatttct cccgcctggg
      601 gagagacggg aagatcggct tcatcgccct gcggcgacgc gacgggagcg ggacgatcat
      661 cattgcgctg gatcccacgg gggcccggta ctggggactg aaggtcgcca tcgagaaggc
      721 catcggagaa ctcggagagg ggcagcgcca gaacctcctt tacctggtcg tcatggaccg
      781 gcagggcaag gtgctgggca gcaccggcca ggggccgcag agctgggggt ccggggaatt
      841 ccgcctgccg gaacttctgg acggccggcg aaagatcgag agccgcaaag gcgtctggca
      901 gggcaagaac atcttggata tggcgatccc cgtgaccctg aacaaccgga tcgagggcgt
      961 ggcgcggatc gggctggacc ggtccgggtc ggacaggatc ctgagggaaa acgagcggaa
     1021 catgctggtc ttcatggcct ttatcgtggc gatcatgctg cttgccatgg gactcctcta
     1081 cttcaatcag aaccggcata tggaacggct cgtggagatc acccggcagc tggagaaggc
     1141 cgagcgtctt tccgcgctgg gccagctggc cgccggcgtg gcccacgaga tccgcaaccc
     1201 gctcaacgcg atcagcatgg cgagccagag gctcaagcgg gagtacctgc ccgacaaccc
     1261 ggacaaggtc ggggatttcg gcatcctgac cggcgtcatc cgggacgaga tccggcgcct
     1321 gaacggcatc atcgaggaat tcctgacctt ctccaagagc cggcgcctgg aactgcggga
     1381 gtattccgtc gtcgaggtcc tccagaaaat cgtcaatctc atccgggagg aggcggtgac
     1441 gaaaggcatt gccctggaaa cccactgggg ggaggatccg ctggtcattc ccatggatgt
     1501 ggacaagctt cagcaggcca tgctcaattt catcaaaaac gccatggaat cgatcagcgg
     1561 cgcggggacg gtcatgatct ccgcggcgct tcagagccgg gagcgcctga tcatcgccgt
     1621 gtccgatacg ggatgcggca tgaccccgga ggaggtggag cggatcttca atccggaata
     1681 cacgacaaag gaaaaagggc tgggcctggg gatgacgctc gcgcacgaga tcatccgggg
     1741 gcacggcggc gagatccgcg tgaagagcga ggccggtgcc gggaccacgg tagagatcat
     1801 cctgccgacg gaaagccgga aaagctgaag gaacacgatc agggggaatt gaggcatgaa
     1861 ggcattgaac atcctcgtcg tcgaagacgg ccagtcccag cgcgaaatgc tccgcgattt
     1921 tctgcttcgg gaggggcacc gggtcaccga ggccgagacc gggccggggg ccgtcgaggc
     1981 cgtccaccgg ggacaggtgg atctcatcct gctggactac aagatgccgg gcatggacgg
     2041 gatggaggtg ctgaaggagg tcaagcgtct gaatcccgag atcgacgtgg tgatcatcac
     2101 cgcctacggg acgatcgaga cggctgtcga tgccatgaag gcgggcgcca tcgattacat
     2161 caccaaaccg gtcgaattcg acgaactgct cctcctgatc gaccgggtct gcgagcggcg
     2221 gatcctgatc cgcgaaaacg agattctccg gcagaacctt cgagagaagg gggtcaccgc
     2281 cgagcaaatc gtctacgcca gcgaaaagat gaagaccctg atcaacctgg cggggcgtgt
     2341 ggccggaagc cgcgccacgg tgcttctgca gggggaaagc ggtacgggga aggagctctt
     2401 cgcccggctg ctgcaccatt tgagcccccg ctccgggcgc cccatcatcg ccgtgaactg
     2461 cggggccctg cccgaaacgc tcctcgagag cgagctgttc gggcatgaaa agggcgcctt
     2521 caccggtgcc ggcgcgcggc gcatcggccg gttcgaggag gcggacggcg gcaccctgtt
     2581 catcgacgag gtcggcgagc tgtacccgtc cgttcaggtc aaactgctgc gctttctcca
     2641 ggagcgggag ttccagcggc tgggaggaaa tcagacgatt ccggccgacg tgcggattat
     2701 cgccgccacc aaccgcgacc tcgaggcccg cgtccgggag ggggcgttcc gggaggacct
     2761 gttctaccgt ctgaacgtcg ttgttctgtc catcccgccc ctccgggagc gcaaggagga
     2821 cattcccttc ctgatcgatc acttcctgca gcgcttcgcg acggaaaacg gcaagcggat
     2881 cgaaggcatc agccgggagg cgcgggacct gctcctcaag tacgcctatc ccggcaacgt
     2941 ccgggaactg gagaacatca tcgagcgggc ggtcgtcatc tcgagggaat ccgtgatctc
     3001 cgtggaggat ctgccctttg gcggcggggc gccggaggtt cgggaggnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnntcg ggagggaggt gtcctccgcg attccgtcga
     3181 ggacctggag cgccgcctca tcctggaggc gatggagaag gccggggacc accagacccg
     3241 ggcggccgag attctcggaa tcagcgagcg gatgctgcgt tacaagctga agaagtacgg
     3301 cctgaaatcg taaccgcccc gaagttttcc tctctgaaga aggcgcgcgg ccgatcccgg
     3361 ctttcctgag cgcgtcgacc ggatgccgcc cacgggccat ggcccgaatc cgggcggatt
     3421 atcccgtttc tccggcgatg tcggcgatcg ctttgtccaa gaggcggacc aaggcattga
     3481 tctggcgttt cgtgatgacg aacggcgggg caatgaggac atggtcccca cggatcccat
     3541 cggccccgcc cccgccggaa tagacgatca gcccccgggc gaaggcccgc tccgcgatgc
     3601 gcgcatggat ccgggcctcg ggggggaacg gctcccggga cgcccggtcc cggacgaact
     3661 ccacgccggc gaagagtccc agcccgcgga tgtccccgac gatccggtgc cggttcaggc
     3721 gcgccaggct gtccagaaga tagggcccca gcgccgcgga gcgggcgatg aggtcacggg
     3781 cctcgatgta ttcgagcacg gcgagagcgg cggcgcaggc caggggattc tgcccgtagg
     3841 tgtggccgtg gacgaatgcc ccccggccgg tgcggatcgt ttcatggatg gcctcggtgg
     3901 cgagcaccgc gaagatgggg ctatagccgg cccccagtcc cttggcgcag gcgatcagat
     3961 ccggtttcac cccccagtgg tcgacggcaa aggcctttcc cgtccggccg aggccggtca
     4021 tgacctcgtc ggcgatgaac aggatgtcgt agcggtcgca gatttcccgg atgcgccgga
     4081 agtatccgtc ccgggggacc agcgccccgg ccgtcgcacc gacgacgggc tcggcgatga
     4141 atgcggaaac ggactccggg ccttcgtgga ggatcgtctt ctcgaggtcc gtggcgcact
     4201 ccagggtgca ggacgcgggc gcaagaccga agggacagcg atagcagtag gcgggagcga
     4261 tgtgcggggt atgcgggagc agggggaggt aatcccgacg acggcccgtg tggccgctta
     4321 aggccagcga ccccagggtg ttgccgtgat agctcgtcca gcgggagatg accttgaatc
     4381 gggagggctg gccgcgatcc acctggtact ggcgcgagag cttcagcgcc gtttcgaccg
     4441 cctccgatcc tcccgaaacg aagtaggccc ggttcaggcc ggccggggcg agggcgacca
     4501 accgcccggc gagggtgacg gccgcctcgc tcgtgaactg ggagccgtgg gtgaaggcga
     4561 cctttcgcaa ctgccgcacg acggcacgct cgatgtcctt gacgccgtga ccgatggcgg
     4621 tcacagccgc gccgcccgct ccgtcgagat accgtttacc gtccgtgtcg tagaggtaga
     4681 ccccccggcc ccggtccacc gccgggtaga agcgggccgg attccggtag aagacgtgat
     4741 cctggttgag ccttccgcgc ttcatccgtc cccgtcttcc gccagtgcct ccacccgcgc
     4801 cgcggtccag cgccatcaat cctgccagcg gctccaggta ttcaccagga tctggcgcgg
     4861 gccgagttcg atgggcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc
     4981 cagcggctcc aggtattcac caggatctgg cgcgggccga gttcgatggg cgcttcacgc
     5041 gtaggggtgg cgggcgagaa aacccgccgt gggcatgggc cgtctccgct acttctggaa
     5101 ccgcttcttg atttccgata ggatgccgaa gtgccggcgc tcctcctcga tcacccggtc
     5161 cacggcggca tgttcggcgg cgggcaccac ctgcttgatc tcgtggtaat agaggatgga
     5221 gtcctgctcc cggcgcaggg cgaaatccag ggccgacagg gtatccttca cccccgccag
     5281 ctcacggtcc agggcctcct tcgtgaagat caccgtgttg tccacgtagt tccgcagata
     5341 ggcggcgtac tcgcccgcat agctctccgg cggggcggtc ttctccatgg cggcgaagat
     5401 ccggccgaag gtcttctggt ggttcagttc ctcgtcggcg aggcgcgcga aaatctcctt
     5461 cgcctcttcg tccttggtga tctggacggc gtaccggtag aaattggccc cgttttcttc
     5521 gatcctcatc gcgaattcca gaatgtccga tgctgagaaa ctcatgtact gcctccttcc
     5581 cttgtccacg ttctgcgggc cgccacccgg ggaggccctt gaaaaacgcc gcgtccgtgc
     5641 cacgccgacg caggcgaaat cctatccgac tttggcccag aattaaagaa aaaaatctac
     5701 ggcagccacc gtttgcgcct gaaatacagg agcatggccg cgacggcgag gaggatgacg
     5761 ccccagacgt agaggtatcc ccagcgccat gccagttccg gcatgtactt gaaattcatc
     5821 ccgtagatgc cgacgatgaa ggtgagcggc atgaagaggg tggcgatgat ggtcaggacc
     5881 ctcatgacct cgttcatccg gttgctcagg ctggacagat agatgtcgag catgcccgcg
     5941 agctggtcgc ggaaggtttc gatcgtgtcg atcatctgga tggtgtggtc gtagacgtcc
     6001 ctgaaataga tccccgtgcc ctcgtgaagg agcgtcgagt cggagcggtc gacggcgctg
     6061 atcgcctccc ggaggggcca gaccgatcgg cgcagggagg tgatttcccg tttcagcccc
     6121 tggatggcgc gcagggtatc cggcgtgggg cgggtcatga ggcggtcctc caggacctcg
     6181 atccgctcgc ccagggcctc cagaacgacg aaatactgat ccacgacggc gtccacgatg
     6241 gcgtgggcca ggtaatccgc cccgcccttc cggatgcgac ccttcccggc gcggagccct
     6301 tcccggatcg gatccagcag cggtccggcc ttctcctgaa gcgtgatcag gaagtgcggg
     6361 ccgatgacga ggctgatctg ttccgaacga atgcctccct ccggattcgc ggggagcatg
     6421 aacacgcgca tgacgatgaa gaggtaatcc cccagatcct cgaacttcgg ccgctgttcc
     6481 gtgttcagca tgtcctccag gaccaggggg tggagtccaa acgcctcgcc cagcgccgcc
     6541 aggacgcccg tatcatggag accgtcgata ttgagccagg tgacgctcgg ccgattccga
     6601 tagggcacgg cgtcgccgac ggcggcgatg gtccgctcct cgatgtgctg ctcgtcgaag
     6661 ttgaacaggg tgaggcaggt cttttcggtc aaccggcggc cgacgtggac cagggatccc
     6721 ggcggtaggc cgaccttgcc cactcgcgtg ggctgcgggg acgacatcgt cagggcgcct
     6781 ccatggggtg atggggtgtt ttagcccaaa gctccgagaa atacaatcga aatttgaatg
     6841 ccttatcgca ccggcgccgg agtttttgct tgacaagacg gagataattt tggagacaaa
     6901 cagggacccc gccggcgatc gccggatgcg ggaggagggg catgaaaggc atccttgtcg
     6961 ttgtacccga tcgcaacgtc gtccaggtcg tccgcggcat cttccgttcc cgggggacgg
     7021 tggacgccgc ggatgacctg gatgcctgcc tcgggctttg ccggaaaaag cgctacgagc
     7081 tggcgctgat cgatcttgcg ctgctgcagg cgctgtcatc ccgccacggg cagtacgact
     7141 acaaatcctc cctccagcag ctctggcaga ttcagcccgc ccttgagatc atcgtcatgg
     7201 ccggacagga cgcggtccgg gaagcggtga tggcggtcaa ggcgggcgcg agcaactacg
     7261 tgacctatcc gctcaaccgg gacgaggtcc gatatgccgt cgaactcatc gccgaggaaa
     7321 accggacacg ctcggagctg gattacctcc gaagccgctt ctggcaggcc gacgccctcg
     7381 aggtggtcca gacgaagaat gcattcatgc ggtcggtctt cgaggccatc cgcctggtcg
     7441 cgccgaccaa gagtacggtg ttgctcaccg gggaaacggg aacgggcaag ggcattctgg
     7501 ccaagctgat ccactgtcac agcaaccgta aggacgtcca gttcatcggc gtccactgcg
     7561 gcgccattcc cgatacgctc ctggaaagcg agctgttcgg tcacgagaag ggggcgttca
     7621 ccggcgccgt ccggcggaaa ttgggtaaat tcgagctggc ccacggcggg acgatttttc
     7681 tggacgagat cggcaccctt tcccccgccg cccagatccg gctgctccag atcctccagg
     7741 acaggaccta tgaacgcgtc ggcggcgagg aaaccctgga ggcggatgtt cggatcgtcg
     7801 ccgcttccaa tgtggatctg aaaaaactcg tggaggaggg cctgttccgg caagatctgt
     7861 actaccgcct gaacgtcttt cccatcgagc tcccgcccct gcgggagcgg acggaagaca
     7921 tcccccaact tgtggaagtc ttcctcagaa acctgaatcg gttcaaccag aaggacatcc
     7981 attatgccca tccccaggtg atggacgcct tccgccggta tccctggccg ggaaacattc
     8041 gggagctcga gaatctggtc gagcgggcct atatcctcga gacctcgtcg atgctgacgc
     8101 ccgagagctt cccggcggaa cttttcaccg tgaccccgac gcccccgcgc ctgtccctga
     8161 acacgtcgct cacgctgggc gaggtgcgaa ggcaggcggt ggaggccgtg gagaggcaat
     8221 acctgaaaga actgctgtcg gtcaaccggg gccgcatcga ccgcacggcg gtcgcggcgg
     8281 gcgtcacggg ccggcagatc cacaagctgc tccagcgtca cgagatccgg aaagaggact
     8341 tcaagtagcg tcctgtccga cgatttcaac ggtcacccgg acggtggcgg ccgtgtccgg
     8401 ggcgagctgg acgtcggagg gtggcgtcag cttcacctcc acggtccggg tctcgcgcag
     8461 gcgttgcagg ttgaccgggg ccgttttgat ggcgatcttg tccgtccgcc gcttcgaagg
     8521 gacgaggatc ctcacggtct ccggcgacac cgtaatccgc cccacggcgt aacccggcgg
     8581 cggatggtgt tcggtcgcga cccgcacggg gacggagaca ggcaccagtc gcgaggccga
     8641 aagcacgagg tggttcggct tgatgtcgag aacagtgagg ttcgcgggaa cgcgcaccat
     8701 gtttctggtg agcgggattt cctgccttcc ctcccggagc cgggagaggt ccagggaaat
     8761 cttgagcgag ccgacgtcca gaaactggaa ggcctgcgac ggccccatga ccatgatcgt
     8821 ggcttccggg atcttcggat tctcgataac ccagtcggcc gcaatattga cgtactcgac
     8881 gggagccaga aaatcccgac ggacggagtc gcgctggtat ccgaacacga gccagaggac
     8941 gcaggccagc agaattgcga tgaccttctc gcggggattc gttttcaggc gctgccagaa
     9001 ggggtgagcc gcccggacgg gcatgcgggc cgcgtagaag gcggaaaggg cggatttcag
     9061 ggccgcggca tggggcagga cctgaatctg ttctccccgc gcgagggaga tcgtgccccg
     9121 ctcttcggag accacgatgc agacggcgtc ggaccgttcg gccaggccga gggccgccgt
     9181 atggcgcaat cccatcggct gatttttccc cgcgccggtc gacaggggga gatggcagcc
     9241 gaaacggacc acccggttgc cgtcgatgat cacggccccg tcatggcccg cggaatgggg
     9301 gtcgaaaagg ctctccagga gcggctggga aagcagaccg ttcagccggt tcccgccgct
     9361 caggtgacgg tccagcggat cgcttccggt caggacgatc agggccccga tatggtttcg
     9421 ggcgaggttc gccgccgtca gggcgaggat ttccgcggtt tcttcatggg cgacgggttc
     9481 gcgccactgc tttcggaagc gtccccagag ggcgagacgc tccaggaaac ggcgcagatc
     9541 ctcctggaag aggacgacga gggcgaaggc caggatgccg aagaacccct ggaggacgac
     9601 cgtcgtcagg tacagatggg aaaaccgggc cgcgccgtag aggaagccga gcaggaccac
     9661 gccggagaag acgaagcggg aggcccggtc cttgaaccag atcagcagga tggcgatcat
     9721 cccggcgatg atgacaatgt caaacaaatc ctggaggcgg aagttctgga agagggttgc
     9781 catttcgtcc atggtgtctc catgactggg ggcagcgccg ggttccggcg cgaaggggac
     9841 gttcgaatct cccttctctc atacggaatt cttcggggag gtgtcaataa caatatgcga
     9901 cggcggatga aaaaaatcgc catgtgcgtc ctgctgttcg ggatcgccct cctgtcgctg
     9961 gtcgtcccgg ccccgggcgg tgagacgatc tccctcagcg gcatggtctt tgttgtggac
    10021 tgggacgagg aggaccggat cacggcggtg tccgtcgtca ccgaggaagg acgggagtac
    10081 cgcgttctcc ccgacgcggt ggggagggag ttgttccagc gcgaagggga cacggtggac
    10141 atcgagggcg ttgccggtac ggatgcggcg ggtcgaaaga ccttgacggt gatccgctac
    10201 cgaatccgtc cgtgagcggg ctggtcacgc cggttttcgc ttggtctttt tcctgaggat
    10261 ggtgtatctg taggacatca gcggactgtg aaggaggcgc ccgtggccga catccatctg
    10321 gacgaaaaga tcggggccgt tgccggcatg atcgtggcgg ccgaacgcgt ggtggtgttc
    10381 accggcgccg gcgtcagcac ggaatcgggg attcccgatt ttcgcagtcc gggcgggatt
    10441 tggagccggt tcgatccgga ggacttcacg atcgaacgat ttctggtgag cgcggagacc
    10501 cggcgaaagc aatgggagat cctgctggac gggggactgc tcacggacgc ccggccgaac
    10561 aaggcccacc ttgccatcgc ggagctggag gcgctcggca agctgacctg cgtcattacc
    10621 cagaatatcg accagctcca ccaaaaggcg ggcaaccgtc cggagaaggt gttcgagctt
    10681 cacggcaata tgcaatggct gcgctgcctg ggctgcgaca cgcggtatcc gctggacgaa
    10741 atgctgagcc gctaccgatc ccaccccgag gttcccgtct gcgaacgctg ccggggcatc
    10801 ctgaagccgg acgccgtctt cttcggggag gccctgccgg aggccgcctt gggcgccgcc
    10861 tcgtatcacg cccgccactg tgacctcttt ctcgtcgtcg gttcgtcact cgtcgtttat
    10921 ccggcggcct acatgcccgt gtatgccaaa ggggcggggg ccaagctcgc gatcatcaac
    10981 ttgagcgcca ccctgtgcga cgaccaggcg gatattctga tcaacgcgac cgccggtgac
    11041 gtgatggaac gaatcctgcg ggacgtccgc gcccgcctct agcggcgcgg ggggcgacgg
    11101 gagggcgtat gtgcctcatt ctctttgcct acgggatgca cccccgccac cggctgattc
    11161 tggcggccaa ccgcgatgaa ttctacgcga ggccctccgc ggcggccgcc ttctgggccg
    11221 acgccccgga tcttctggcc ggacgggacc tgcgggacgg cggaacctgg ctggggatca
    11281 cccgccaggg gcgattcgcc gccttgacga atttccgcga cccggcatcc ctgaaaacgg
    11341 gggccccctc gaggggacgg ctggtcggcg acttcctccg ggggcaacag gcgccgccgg
    11401 actatctgga tgcgatccgc gccggggctt ccgcgtataa cggattcaac cttctggtgg
    11461 gcgattttcg ctccctttac gtcttttcca atcggggcgg gtccatgaag ctgtccccgg
    11521 gtttgtacgg cctcagcaac cacctcctga ataccccctg gccgaaggtg gaacggggcc
    11581 gggcgttcct gcgggccctg ctggagaacg ggggcgaccc cgaaacggaa gcgctctttt
    11641 cgctcttggc ggaccgaagc cggccggcgg acgcaaacct gcccgatacc ggcgtcggtc
    11701 cggcgtggga gcggatcctg tcgtccatct tcatcaccag tccggtttac gggacccgtt
    11761 cctcaacggt cctgaagatc ggtcataaag ggggcgtgga gttcgtggag cgcgtcttca
    11821 acggccggcc cgaggcctgg atgacgtccg ccttctcctt ttccgcgcta taagcatcgg
    11881 agagatccga ccccacggcc cgtgacaggg cccggacagg aggtgaatga cgatgaaggt
    11941 tatcttccac gaggatttct acgaggtcta tgccggggat ccggcggcgg cggcgggacg
    12001 catggaggcc gtcgtcaagg cgatcgaggg gcgcgttacc tttgtcgagg ccgtgccggc
    12061 cgccgaggag gacatcgcgg ccgtccacac gcgctcccac atcgaacagg tgcgccggga
    12121 gggcgtttac accgtggccg ccctggccgc cggcggggcc atccaggcgg cccgcacggg
    12181 gctgaaggag ccgtgcttcg gcctgatccg gcccccggga caccacgcct cggcaggcag
    12241 cgcctggggc ttctgctact tcagcaacat ggccatcgcc ctggagcggc tgcggcggga
    12301 cggtctcatc aagcgggcct tcgtgctgga catcgacctc cactacgggg acgggacggt
    12361 caatgtcctg gagggccggg gctatgtccg gatctacaac ccgtccacga acgaccggaa
    12421 cgattacctc aagcgggtgc gggacaagct ccgggccgag ccggtcgata tcatcggcgt
    12481 ctccgcgggt ttcgatcacc atcgggaaga ctgggggggg ctcctgacga cggaggatta
    12541 ccgcgagatc ggccggatgg tgaaggaggc gggtgaggcg catggggcgg gccgcttcgg
    12601 catcctcgag ggggggtaca accatcgcgt cctggggcgg aacgttctcg ccttcctcca
    12661 gggactggag gggggcgagg agaattagct actcctcctg aagctgttcc ttgagtttct
    12721 tctggttctg gagggcctgg ccggcccggt tcagggccag ggtgatgttg tcgtccggct
    12781 tgcccaggag ccggtccgcc tgccgataga ggcggagcgc cgcctcgaaa tcaccccggc
    12841 tttccgcgca cacgccgagg ttgtactgga tggccacggc gtcgggcgaa aggatgcgcg
    12901 ccgcccccca cagttcgcag gccatgtcca gccgtccctt gccggcatag gcgatcccct
    12961 gcttgagctt ttccttggcc tccggagcgg tgatgccgtc cccggtgtcc atgagcgtga
    13021 tctcgcgggt gacgtagtag ggggcgatgt ctcttcggaa ctgggcttgg gccgtttcct
    13081 tggcgcgggt gaacagggcc tcgccgtccg cggcgggccg cgtatcctcg cagccggagc
    13141 tctcggcggc gccggacagg ttgcgggcgt acaggacccg gcccgtttcg acctcgacga
    13201 gtttcggcgt tccggaaatg ctggccgttc gtttgatgca gttcacgtag aaggtccgcc
    13261 agcggatgca ggcaccctca tagagacgcc cgtcctttcc gcgccggacc tcccgcttga
    13321 cgcactcgct ccgcttggcc ttgaaggggg tgtcctgcca gccgtaggcg ccgacaacgc
    13381 cggtatagat tccctgcgcc ccgaccagcc ggccgatctt cacggccgtt ttcgggtcga
    13441 tcaggccgct ctgggagagt ttcagctcgg agacggtctt gtcgatcgaa gcccggtcca
    13501 ccagggtgaa gtatggcttg tcgtcgatgt tgatgctgtt caggacgccc tccatctcgg
    13561 cggcgaactc cgccccaccg ggaccgtcga agggcaggac ggccacgacc ttcgtgagag
    13621 aggcttcgtg gtaggccgcc ggctgcagca tgttcacccg gatctgagtg gcgcaacccg
    13681 tggtgaacag gaggagcagg ataggcacga ggcgggccgg agacggcgaa cggcgggtgc
    13741 gggttcctga catgggggct ccttcattcc tcgtcggggg gccggcggtc ggaacgggac
    13801 ggctcgccgg tgtacttcag ggtcagggag tagggcgaaa agttcacgac atcgacgccc
    13861 tctttctgat tcaggctgtt ggccaggtag agcgtgttct ccgggtagct gaccacgaac
    13921 tgccccatcc ccttttcctc cacgttcgtt acccagacga gcgtctgaag aagatcctgg
    13981 acctccatgt tcgcgtcgag ggtcttgacc ccctgcacct cgatgcgcac cttcttcacg
    14041 ttcccctgga tgtactcggc gaccttgtcc agaagcttcg gcgtcagcac ctccgccatg
    14101 tcctccatgg ctctggccgc ggcgtcctca acgctgttgg ccagcccccg ggcctgttcc
    14161 accccggcgg tgagaatctc cattttgccg gtcttgttgt ttcgggccac catccggtac
    14221 gtcaggcgca ccgtcacgtt ctggaagggc atgacgatcc cgtagccgat gtcctccccc
    14281 tttttcacgg agatgttata gtcgagcttg ccgatgatga tcaggttgga gaggaacctg
    14341 tacatcaggc ttcgcaggag gacggaactg ccgcccttgg cggccttctc gatcgccgcg
    14401 gcatcggcgg cgccggtggg ggccacgtcc accacggagt agccctgctc cgtcagccgc
    14461 ccgatgagcg tctccgacag gatgttcgtc tcttcgaagg cgtcctcgtt ctcccgggtg
    14521 ttcggcacgc gggccggaat gaaaacggcg accgcgctgt tgagcgcgag cgcggagagg
    14581 gcgtcccggg cccgggtcgg ctcgacgcag gcgttgatct tgacgacgac cacatcgtcg
    14641 cgggtctcct gcctgaggac tttgaagcgc cggaccgcgc cgccggtctg ggttttgatg
    14701 gcatcatcga ccagggtgaa gttctgaacg aagctctggg ccttcacttc cgtccccacg
    14761 gtccgttcga tggccgccca cttggcccgg gcgatggctt caaggcgggc tgagggcagg
    14821 tcgttcccga tgacgatggc ctctccctcc gcgtcgacgc acttttcctg gccgaagccc
    14881 ggcgcggcca ggaagaccag aacacagagt ccaaccgcga gacacaccag ggttttgcgc
    14941 attattccac ctcctgagat cgagatcgaa gcctgtatgc cgcatgccgg gacggcatgt
    15001 tcaatccttc cccgcggccg tcggaccgtt acggggggaa gacgaccgcc gcggtcgcca
    15061 cgcgcgcccg caggatccgc gtgatcgctc cccggtcccg ggcggtccgg acaggggtga
    15121 ttcgggatcg ggcggggttt tcctcccggg gcccgtcgcc cccggttccg atgtaacccg
    15181 ggacgatcag gatccggccc gttgccgttc cgcggcaggc gcttccgacg gcaatgtccg
    15241 ccaggacata gagcacgtcg cgatcgtcat acgggatccg tccctcccgg cgcaccgtgt
    15301 attcgcccca gggcgcgcgg acctcggttc ccggaggctg atggacatgg cgccgcgttg
    15361 cgttcacgag aagttgatct gcgagtaggg gtagcgcgcg tcgaggaatg cgcggatcgc
    15421 cgcctgctca cgctcgtcgg tgatgggttc cacgtcgctg accggcatgc cctgatcgtt
    15481 ggttctgacc ttccaggaac ggacgtaacc ggggatgaaa agaaaccggc aaccggaacg
    15541 gccacagcaa gcgttgtcca gggtcgcgat ccccagggcg tagagaaact ccctgccggc
    15601 gtgcgtcagg cgcccctctt cgatgatctc gtagtgaccc gcaatgaaat ggacttcttc
    15661 acccacttcc cggtgcgtat aatccgccat ggtttgtgcc tcccgtgact tggttgagtg
    15721 aatggatggt caagggcgaa cggcgcccgc ttactttccg atacagaagc ccgcgaagat
    15781 tttgtcgagg atttccggag acgccgtcct gcccgtgatc tcccccaggc tctgcaaggc
    15841 gtcccgaacg tcgagcgcga tgaattccgg cgagacacgc cgctccaggg cctctccgcc
    15901 ccgctccagg tgtgtcttcg ccgcctccag ggccagcttg tgccgcaggt ttccgatcag
    15961 ggcgccgtcc cttgtcgccg gttctccacc gcacgaggca gcatatagca aagccttcaa
    16021 ggcgtcaatc ccctcgcctg ttttggccga gatccacagg ggcgggtgga gcggccggaa
    16081 ggcgtggagc cgatcctccg ccaggacatg gggtagatcg gccttgttga cggcgatgac
    16141 cgtggggcgt ccgtgcattt ccccgagtag cgcctcgtct tccggggtga gcggcatgct
    16201 gccgtcgaga aggagcagga tcgcgtcggc atgggacagg cgccggcgga ccatcgcgac
    16261 gcccgcctgc tcgatggcgt cctccggcgt gcggatcccg gccgtatccg tcaggcggaa
    16321 cgggatgccc tcgatcagca gggtctcctc gatgaagtcc cgggttgtac cggggaaggg
    16381 ggtgacgatg gcccgctcct cgcccagcag gcggttgagg aggctcgatt tgccgacgtt
    16441 gggccggccg gtgatgacga cccggaaccc ctcccggcgg acccggccag cggcgtaagt
    16501 gccgagcagg gtccggaggt cctccaggat ctcgccgaag cgcgccgcca gggctccggc
    16561 gtcgagcgcc gtttcgccct cctcgccgaa gtcgatggcc gcctccagca ggacgagcat
    16621 atcggtcgtg cgggcgtaga ggccctccac ggcccggctc aggcgaccct cgagatggcc
    16681 gagggccagc gttgcggccg gggcggagtg ggcgtcgatc aggtcggcga cggcttcggc
    16741 ctgcgccaga tccatccgcc cgttcaggaa ggcgcgccgg gtgaattccc cgggttccgc
    16801 cggccgggcg ccggcgccga ggatttccgc gagcagggtt cggaggagga ccggcccgcc
    16861 gtggccgtgg atctcgacga cgtcctcgcc cgtataggag cggggggcct tcatcaggac
    16921 caggagcacc tcgtcgatgg gttcgcccgt ttccggggag tgaatgcggc cgtggtagag
    16981 gcgatgggac gcgagggagg tcagggggcc gtcggtgcgg aagaggtgtc gggcgatggc
    17041 ttccgcctcc gtcccgctga tccggacgat gccgacgccc ccccggccgg ggggcgtcgc
    17101 gacggccgcg atggtgtcat gaatgttcat gggggtcggc ctgatcgcgg ggcggccggc
    17161 tgcgtgccgg catgatgatg atcttccggt attccccttc gccccggctt ttggtggcca
    17221 actgggtgtc gttctggagc gccagatgaa tgatccggcg gtcgtgggcg ttcatgtgac
    17281 tcaccgtcag gggcttctgg gtcttgcgga ctttttcccc gaggcgttcg gccaggctga
    17341 ccagggattc ttcgcgccgc tggcggtagg tttcggtatc gacgatgatg acctttttac
    17401 cgttgcccgc cttgtaggcg gccttgttca cgatgtactg cagggcgtcg agattctgcc
    17461 cccgcttacc gatcagcagc ccgccgccgt cgccctggat gtcgaggcgg atggcgtcct
    17521 ccgtctcctg gacggaaacc gggtacttca ggtccatccg ctccaggatg ccctccagaa
    17581 ccgccttggc ctgaagggcc acgtcaccgg cgggttcggg gtccggcgtc gcggccgccg
    17641 cgcggctttc gacggggggc ttctccgcgc cgcggtgggg tggggcaggc gaccgcttgg
    17701 gacggggggg cgtttccgga gccgggcgcg cggcgggttc ccgtcttcgc ccggggggcg
    17761 ggggggcttc cccgaccggc tgcggggcgg gccccggttc ttgtttgaat aaagaagtgc
    17821 gttccggcgc cggaacatcc ggcaccggtt ccgcggtcgc gtcctcgcgg tcgtccagat
    17881 ccagggaaag gttcatgagg ccggcccgga tccgggcctt ccgcgcgccg atcccgagga
    17941 atcccgctgc gccttccgaa aggatctcga tctgaagctt ttcccgggga acggcgaatg
    18001 cctcgcaggc ctttacgatg gcctcgtcta tggatttgcc ttcaatttcg atggatttca
    18061 tggcgtcacc tcgtatcagg tgcttctctg gttgatgtag tactgctggc cgatgctgaa
    18121 caggttgttg aagagccagt agatgacgag ccccgagggg aaatagagga acaggaaggt
    18181 aaagatcacc ggcatccaga gcatgagctt ggcctgcatg gggtcgccgg gggcggggct
    18241 catcttctgc tggatgaaca tcgttgcccc catgatcagc ggggtgatga aataggggtc
    18301 ccgggcggag aggtcctgga tccaccagaa gaagggggag tgccgcagtt cgatcgcgta
    18361 cagcagggcc ttgtaaagcc cgaagaacac cgggatctgg atgatcatgg gcaggcagcc
    18421 ccccagcggg ttgaccttgt gcgccttgta gagggccatc gtctcctggc tgatcttggc
    18481 cttgtcgtcc ttgtactttt cctggagggc cttcagtttc ggctgcagct tctgcatctc
    18541 cttcatggac tggtagctct tgttgccgag gggccagaag acgagcttga tcagggccgt
    18601 caggatgata atggcgatgc cgtagttcgg gatgtactgg tagaggaact tgaggacgat
    18661 cagcagcgga aaggccagcc acttgagcca ggagccaaag tcgatggcgt tttcgaggcc
    18721 gaccccctgt gcctcgagga tggtgtaatc cttgggcccc aggtagacgg aatagacgaa
    18781 cgaaccggac tgccccgccg ggatgatcgt gttcgcgccg cgcagactga ccgtgaccat
    18841 gtcccgcgtg tcccggtcga tggcgacgct cgagagggac gggttctggg ggatcatggc
    18901 ggcgaggaag tacttgctct caaagccgcc ccaggagacg tccggcccgt gaattttttc
    18961 acgctcgatc ttcttgacct cgatccggtc aatgctcttg gctacgtagg taaccggccc
    19021 ctcgttgccg tagcggtccg tctccgcctt gggatcgacg tagcggcgcc agttcagggc
    19081 ggcatgctgg ttcagcggat tgccggacag gttccgcacc cggacctcga ggtccacgac
    19141 gtagcggtcc ggccggaagg tgtagatctt ctcgacgcgg gccgtccccg ggaggatctg
    19201 ggtgaagacc agcggccgcc cctccgtccc cgttgtcagg tcgagggccg ttgtgccggt
    19261 tgtgaatccg ccctccgccg gcaggggaag gctggagtcg gggaaggaga ccgtcaaggg
    19321 tcggggcatc ccctcctgca cgtgcaccag ttcgacgggc agggcgggtt tctcctcttt
    19381 tttcttgaag ccgaccaggg cgagcaggcc gtcgaacccg tacctccggt cctcgggcag
    19441 ttccgtccgg tagcccttga gctggaagga tttcagcgcg ccgccccggg tggtgaggac
    19501 ggcggtatag aggggcgttt ccacggtgac gtcgcgctcg gccacggcgg cggcccgcac
    19561 ggccggcagg ggcgccacgg gcggggcaga cgtagccgcc ggagacggcg gaggggcgga
    19621 cgcggtccgg tccggctggg tcaagcccgt ctgcggcggg ggcgttgccg ccggtttcct
    19681 ctgggggacc ggtttgacga aaaaggcctg atagacaaag atgacggcga gggagaggac
    19741 aatcgccaga aatgtccgct tgtccatttc atacctccag gggtctaaat atccttactt
    19801 gacgggatcg tagccgccgg ggtgcagggg gtgacagcgg acgagccgcc aaagggccag
    19861 tccgaggccc cggaggggac catgccgccg gatggccgtc agcgcgtact cggagcacga
    19921 cgggtaaaaa cggcagcagg gggcaaaaag gggggaaagg aaaagctggt acaaccgaat
    19981 caagccgatg aacattttgc cgatgatcgt ttcaagcatg ggtttcatga tccgccggcg
    20041 caaaccggag acggcccagc tcccggcaga catcccggta agtcatccag gggatgcctt
    20101 tcttcgccat gatgaccatg tcccgtgagg ggggcagact cgatttgttc aatctgaagt
    20161 attctctcaa caggcgcttg atgcgatttc gtttgacggc gttcccgacc ttcttggtga
    20221 ccgtgatccc cagccttctg gttccggatg ggctctccag ggtaatgatc gtgaaattcg
    20281 gcgtgtgctc ccgcgtcccc tgctcgtata ccttcaaata gtctcttctc ttgtggatgc
    20341 gttcatgcgg cgcaaaggtc tttcgttcca tggtgtcgaa agcggacccc ccgcggggcc
    20401 gggccgtcat gaggggtaga ggatgctaca ccgtcaggcg cttcctcccc ttggcccttc
    20461 ttcgattgat gacccgacgt ccccccttgg tcgccatgcg gaccaggaat ccgtgggtcc
    20521 ttttgcgctt cacattcgtg actgctgtca ggttcttctt catgatggca ccccgatcag
    20581 ttattaagcg cacccataaa ccacaaacgg tcgtctttgt caagattcaa atgggaatcg
    20641 cgggaattga acgtttcgtt cagcgtgggc gacgtttcgt tcagcaaagg atcgtccgcc
    20701 ggaggtcggg tggggcgaag ggacggtggt acctcattcc ggcccctccg ggggcgaaaa
    20761 ggggcgaggt ccgcgcccgt cctgtcttga cggcggcgcc tttccgggta aaaactttta
    20821 ttatcgatta cttgtgcgct tcctcgggga aacgtccggc ttgtcgaaac ggtccaccgg
    20881 cgccggtgat ccggcgccgg tggcagccgt ccgtttatct tatgggaaag atcgcacggt
    20941 ctggcatgga tctttcatat aacccaagca aaagagatcg accctggaac aaaggaggac
    21001 atcgacaatg aaaaaattca tgcaaacctt gtgggtgacg ctgttcgtgc tcctgacgac
    21061 ggcaaccttc ggatacgccg agttcacggc cgccggcgcg agcgactttc cctacttcca
    21121 tctggggtgc ctgattgtgg gcgggctcat catcgtttcg ctcaagcaca agtacaaccg
    21181 gatctatctg agcgaggccc tcgggtcgtt cgccctgtac acggtgatgg tggccctgtt
    21241 caccgctccg gtcgtcgatg ccttgaagac gctggtcagc tgatcgcgcc gttccctgtc
    21301 cgccatttcg ctccccggtc cccccgaccg gggatttttt tgcccgtccg ccggccgccc
    21361 cccgctcgtc atcgcccgca acaagacgct cgccgcccgg ctctaccggg agttccgcaa
    21421 cgcccccgat aacccttccg tctcaccggt cctgagacga tttgccgttg cgaaaaggtc
    21481 ccgatgtatg cgaaaaggcg gcgagggaag ggatgaacga tggatgcatt cagaatgatg
    21541 accgatttca cgccccaagg cgaccagccc cgggccatcg acgcactgac ggccggcctg
    21601 gcgtccggtc gcccccacca ggttctcctg ggggtgacgg gatccggcaa gaccttcacc
    21661 attgccaagg tcatcgaggc cgccggccgc cccgcgctca tcatcgccca caacaagacg
    21721 ctcgccgccc agctctacca ggagttccgc gcgctcttcc ccgacaacgc cgtcgagtat
    21781 ttcgtcagct attacgacta ctaccagccg gaagcctaca ttcccagcac ggacacgtac
    21841 atcgaaaagg attcgtccat caacgacgag atcgacaagc tgcgccatgc ggcgacgcat
    21901 tcgctcctgg agcgccgcga cgtgatcatc gtggcgagcg tctcgtgcat ctacgggctc
    21961 ggttccccgg aggcctacgc cgggatgctc ctcctgctcg agggcgggat ggagatctcc
    22021 cgcgacgacg tcctgaagcg cctggtggag atccagtacg aacgcaacga catcgatttt
    22081 caccggggca ctttccgggt ccggggcgat agcgtcgaga tcttcccccc ctacgaagag
    22141 gaacgcgcct accgggtgga gttcttcggc gacacgatcg acggcatcgc cctcgtcgat
    22201 cccctgcggg ggaagaagct cgggcccctc tcgcgcttgg ccgtctatcc cggcagccat
    22261 tacgtgacga cccgggacaa cctcgtccgg gccgtcggcg acatccgggc ggagctggcc
    22321 gagcgcctgg cggcgttcca ggcggagaac aaactcctgg aggcccagcg cctggagcag
    22381 cggacccact tcgacatcga gatgatggag gagctcggct actgccaggg gatcgagaac
    22441 tactcccgcc acctgacggg acggcgtccc ggacagcctc ccccgacgct gatggaatat
    22501 ctcccgaagg acgccctcgt ggtcatcgac gagagccacg cgacggttcc ccagctcatc
    22561 ggcatgtacc gcggggaccg cgcccgcaag gagaccctgg tcaactacgg cttccgcctg
    22621 ccgtccgcct tggataaccg gccccttcgc ttcgaggagt tcgagggttt cccccagcag
    22681 cggatctact gctccgcgac cccggcggcg tatgaactcc aggcggccga agaccgggtg
    22741 gtggagcaga tcatccggcc cacggggctc atggacccgg agatcgacgt ccggcccgtg
    22801 acgggccagg tggaggatct ttacgccgag gtccgcgcgc gcgcggaggc gggcgagcgc
    22861 gtcctcgtga ccaccctgac caagcgcatg gcggaaaacc tgacgagcta ctatgaggag
    22921 ctgggcgtcc gcgtccgcta cctgcattcc gacatcaaga ccctggagcg ggtcgggatc
    22981 atccgagacc tgcgtctcgg cgaatacgac tgcctggtgg gtgtcaacct cctgagggag
    23041 gggctcgaca tcccggaggt gtcgctcgtg gcgatcctcg acgccgacaa ggaggggttc
    23101 ctccgttcgg agcggtcgct tatccagacg agcggccggg ccgcccgcaa cgtcgccgga
    23161 agggtcatca tgtacgccga tcgcgtcacc ccggccatcc gggcctgcct tgacgaaacc
    23221 cggcgccgcc gttcgctcca ggagcggttc aatcgaaagc atgggatcac gccggcgacg
    23281 atccgcaagt cgatccacga cgtcctgggg tccatctacg aggcggacta cgtcaccgtg
    23341 cccacggtgg cggaaaagag aaaggcctac gggacggagg aggagatcgc cgccctgatc
    23401 cgcgacctga cggcggccat gaaggcgtcc gcggcggagc tcgccttcga gaaggccgcc
    23461 gcgctccggg acgagatcag ggagctcaag ggcctgctcg tggaactggg ggggaaggca
    23521 tgacggccgg cgctgcttca ccggatgttc tgaaggcgaa ggtcgccctc gcgccccggg
    23581 cgccgggggt ctacctgatg aaggacgggg agggcaggat cctctacgtc ggcaaggccg
    23641 gggacctgaa gagccgcctg aaggcgtatg ccggggggac ggacggccgc gcgatgatcc
    23701 cctttctcgt ctcccgcgtc cgggacgtgg agctcatcct cacggcgagc gagaaggagg
    23761 ccctgatcct cgagaacacc ctgatcaagg cccaccgccc ccgctacaac gtcgacctga
    23821 gggacgacaa ggcctatgtc cacctccgga tcgatcccgc gacgcccttc ccccgtttcc
    23881 aggtcgtgcg gcggccaaaa aaggacggcg cccgttattt cggtccctat ccctccgcgt
    23941 cggcggcgcg cgagacgctg cgcttcctcc agtccgtctt tccactcagg acctgccggg
    24001 acgcggaact gaaatcccgt cgccgcccct gcctggaatt cgagatcagg cgctgcctcg
    24061 cgccgtgcgt cggtcgggtg ggggcggagg cctacgatcg tctggtccgg gagagctgcg
    24121 ccttcctgga aggcgggggg cgggacctca cggacgcgat cagaaggcgc atgcaggccg
    24181 cgtccgaggc cctggacttc gaggaggcgg ccgccctgcg ggaccggatc gccgccatcg
    24241 aggagacgct cgcccgccag cggatggagg acgtgacgga ggccgatcag gacctcttcg
    24301 gcctgcaccg ggagggggac gacctccagg tctgcctgct caccgtccgg ttcggcaagc
    24361 tctccggtca acggctcttc ccgctgatcc gcctcgcggg ggagacggcg gagctcctct
    24421 cctccctcct caaacagttt tacgacgccg gggcccatgt ccccggggag atcctcctgc
    24481 cgctcgatct ggaggacgcg gcggccgtcg cggagtggct ggcggagaag aagggccggg
    24541 ccgtctccct gcgcatcccg atgcgcggca aggcgcgcga tctcctggag atggcccgcc
    24601 gcaacgcgga gaacgccctc cgcacgaagc ggctggccga gttcgacccc gccgaggcct
    24661 tggagatcct ggcccggacc gctggccttg accgcccgcc gcgccgaatc gaggggttcg
    24721 acatctccaa cctcggcggg cgccacgccg tcggttcgat ggtggtcttc gtcgacggcc
    24781 ggccggagaa ggccgattac cggcgtttcc gggtgcggac cgtcccgggg gccgacgatt
    24841 atgccatgat gtacgaggtg ctgaagcgcc ggttttcccg ggcccttgcc ggggcgccgg
    24901 gagacggcgc gcgcccgttt ccccccttgc ccgatctgct gttggtggac ggggggaagg
    24961 ggcagctcca ggtcgccgtc accgttctga aggagctcga tctgaagacg gtggactgcc
    25021 ttgccctggc caaggagcgg caagaggccg tccggatgcc ggggaccgtg gaccgcgggg
    25081 aagaccgggt ctatctgccc cgccgccgcg agcccctcta cctttcccgt aggccggcgg
    25141 ccctgtttct cctccagcag gtccgggacg aggcccaccg cttcgccgtc gcctatcacc
    25201 ggcggctgaa gacccgggac gattttcgct ccgcgctcga cgccgttggc ggcatcgggc
    25261 cgaaacgccg ccgggatctc ctcgccttct tcggctcggc ccgccggatc gccgccgctt
    25321 ccctggagga cctgcaaaag gtggcggggg tgggccgtca gaccgccctc aggcttcgcc
    25381 gccacttcaa cccggaaggg tgaacggtcc ccacccccgc ccgatcgaaa cgctcacggc
    25441 agtttgcaga cggggctgtc cctcccgagg tagacgacga accacatcca tgcgcggccg
    25501 atcggcctcg acaggcgcgt cgtttgcggc aggcaggttc gcctcttgcc cgcggatggg
    25561 ttccgtgggt cggaaaacgg gaaattgcgc gcatccgcgc cggaatatgt ttgtctgcgg
    25621 cggaggttaa cagacgggcg gatcatgacg gggggacgtt ctactgccag ccgatgacct
    25681 catagccccg ttcctggagg caccggtcca tgaagctttt gaacagggga ctcggttccg
    25741 aggccctgat gacgccccgc accagggcgg ccgccgcccc ggtggcggcg ccgacgccga
    25801 cgccccgtcc gagatggccc acgacggcgc ccgcggcgcc cccgatgatg gcgccccctc
    25861 cggccgagat ggccatgtcc ttggccgcct ggatcccggc gtcggattgg acgtactgat
    25921 cggccagcgt ctcgcattcg gcgatatcct catgggcctt cgcctcgccg acctcccgca
    25981 ggtatgaatt ggggtacaga acggggcccg tggcacagcc cgccagggag agaatcgaga
    26041 tcatcagaag gatcttcttc atgtcgttgc ctcctttgcc ggcacgctcc ctgtttttat
    26101 cataacggaa ggaaaaaaca cagacgtatc cgcatcgacg ggcgggtcga tcccggaatg
    26161 cccgaacgtc cggtctgcct ctgccggctt gcaaaatcgg gcggggattg ttaaggagtt
    26221 cgccggacga acccgccgcc gttccaatcc cgaagatggg agcggagcgg gcgaccggtt
    26281 ccgccgggca aggatagagg agagaggaca tggggttcgg cgccattctg atcatcgccg
    26341 tcggcctggg catggacgcc ctgtccgtgg ccgtgggcat cgggggcgcc cgccgggagc
    26401 gaacctgggg accgaccctg cgactcgctt tgagcttcgg cctcttccag ttcttcatgc
    26461 ccgtcgccgg gtggctcgcg ggccgaaccg tggccgacat catcgccgcc tacgaccact
    26521 ggatcgcctt tgccctgctg gcctacgtgg gggggcggat gatccgggag tccttccggg
    26581 gcggggaagc ctcgccgccc gcggacccca cgcggggctg gacgctgttg atgctgtcgg
    26641 tcgcgacgtc gatcgacgcc ctggccgtgg gcctgagtct cgcctttctc aagacgccga
    26701 tccttttccc cagcgcggtg atcggcgcgg tctgcttttt gatgacggtg gcgggcatgc
    26761 gctttggcga acgcctgggc cggctcgtgg gacggcggat ggaggcggtc gggggcctcg
    26821 tcctcatcgg gatcggcctc aagatcctga tcgaacacct ggccggatga cgcgcgtcga
    26881 cgggaaggcc ggccccaccc tttggaaaca gatctctgaa gaacgccttc ggtggtccgt
    26941 ctcacccttt cccgggagcg atgccggttt atttccggat ggccaggttg tcccggtgga
    27001 tgatctcgtc gtagtccttg tggcccagca cctgcgcgat ccgggccgtc ttgagcccca
    27061 tgatcttccg gatctcgtcc gcggcgtagt tcacgagtcc cttggcgacc ggcgccccgc
    27121 acgcatccac gcagaccacc gggtcgccga tcccgaaatc cccctccacg tccacgaccc
    27181 cggacggcag gaggcttttc ccctgctcca gcagggccca cttcgccccg tcgtcgatga
    27241 cgagcttccc cttgcccctc agggtgaagg cgatccagtg cttgcggctg gtcaggtgct
    27301 tttgcatggg caggaagagc gttcccaccg cctcggcggc gaagagggcg ccgaggacgc
    27361 cggggcgctt gcccggggcg atgatgtagg ggatcccgaa ggccgtgacc ttctgggcgg
    27421 ccaggatctt gctgcgcatc cctcccgtgc cgacgtcgga ggtctccgcc gtggcggcct
    27481 ccaggatgtc ctccgtgaac tcccggacca gggggatcaa cctggcctcg cgcgaggccc
    27541 cggggttgcg gtcgtagagc ccctccgtgc tggtcaggtt gacgagcagg tgggcctcga
    27601 tgatgttggc gatcatggcg gccaggtggt cgttgtcgcc gaacttgatc tcgtccaccg
    27661 cgaccgagtc gttctcgttg atgacggcga tgacgcccca ctccatcagc gtggagaggg
    27721 tgttgcggat gttgaggaag cgcttgcggt ccgtcaggtc gctcagggtg agcaggagct
    27781 gggccacgta gagcccgtgg cgcccgaagg cgttggagta gacccgcatg agccgcccct
    27841 ggccgatggc cgcggcggcc tgtttctggg gcatgctctt gagctcgccc gtgatgccca
    27901 tccggtgctt gccggaggcg atggccccgg aggtgacgag aacgacctgg cggccggact
    27961 tcgtcaggag ggcgatgtcg tcgacgagtt gctggacgat ggtcaggtcg aggccgtccg
    28021 cccccgtcag gacggcgctg ccgaccttga tgaggaccct gcggaccggc gccaggacgg
    28081 tttggcgaat gggattcatg gggttacctc ctcggtgttc ttttcgcgcg cggcatcgag
    28141 ggcgtcggcc aaggcgtgga gcagcgcggg aagcccctcg ccggtgaccg cggagaaggg
    28201 gaaggcccgg atgcgctgcc gcaggagggc agtcgtctcc gcttccaggc gctcccgggt
    28261 gtcggtcagg tcgatcttgc tgatggccac cacctggggc ctggccccga ggcccgggct
    28321 gaagtgccgg agctccgcgt tgatcgtcgc catcgtctct ccggccgggg ccgcatctcc
    28381 ggagaggtcg atcaagtgga gcaggacgga ggtccgctcc acgtggcgga gaaacttgat
    28441 gcccatcccg agcccccggt gcgccccggg gatcaggccg gggatgtcgg ccacgacgaa
    28501 gggggcgcgg tcgccgacct tgacgacgcc gagattgggc gtcagggtgg tgaaggggta
    28561 gtcggcgatc ttgggccggg cggccgagac acgggagatg aaggtcgatt tccccgcatt
    28621 ggggaggccg atgattccga tatccgccag gagcttcagc tccaggagga tccaccgctc
    28681 ctcccccggc attcccggct gcgcgtagcg gggcgcctgg ttcgtcggag tggcgaagcg
    28741 ggcgtttccc cggccgccga tgccgccccg ggcgacgatg aacgtctcgc cggcgcggac
    28801 cagatcggcc aggacttccc cgctctcagc gtccttcacg acggtgccgg cgggaacggg
    28861 gatgcggatg tcctcggagt tccgccccgt ccggtcgctc ccctcgccgt ggccgccgtg
    28921 cttcgcgatg tggtgctgcc ggtacttcag gtcgaggagg gtctggtggc tcccggaggc
    28981 gacgaggata atgtctccgc ccttgccgcc gtcgcccccg ttgggtccgc cccgggggac
    29041 gtatttttcc cgccggaagc tgacgcaacc gcgtcccccg tcccccccct tgacatagat
    29101 cttggcttcg tcgatgaatt tcatgggcta ctttctgatg cccagcaggc ggtagatttt
    29161 ctgcgccgcg acggtcggtt cgatctcgaa gacgccgatt tcctcggcgt tcagggaaga
    29221 ctgcgcggat gagcaggcct tctcgctgat gaggatgttg tccggccagg acttgcagag
    29281 ggattgcagt ttgaagacaa aattcacggc gtcgccgatg accgtgtagt ccatcttctt
    29341 ctcgaagccg atgttcccga cgaccacctc gcccgtgtgg atgctgatcc ccatggtcag
    29401 ggtcgtcccg aactgctcct tgaaatacgc gttcaagagc ggcagggtct cctgcatctc
    29461 cagggccgcg gcgacggcgt tgtccgcgtc cctggcgctc gaaacggggg cgccgaacag
    29521 ggcgaggaag ccgtccccga ggtacttgtc cacgatgccg tggttcctga agacgatctc
    29581 ccccatctgg gcgaagaagt ggttcagcat ggccaccgtt ttctgggggc cgagcttctt
    29641 ggccaggggg gagaaggagc ggatatcgat gttcaggagc gtcaggctcc ggaactcctc
    29701 gaccagcggc tgctccgcgt cgctgcgcag gaggatcttg tccaccacct cccgggggac
    29761 gaacttctgg aacaggctgc ggatgccgcg ctccgcctcg gccatgctca gggtctcccg
    29821 gtacaggcgc gcgttctcca tggcgatgct gacggaggtg gcgatcgatt gcaggaggtg
    29881 gatgtcgctg ttgtcgaaga ccccgccgat cttgttcagg acctcgatga cgccgatcac
    29941 ctgcccctgg gaaatcatgg ggacgcacag gacggagcgg gtccggaagc cggtccgggc
    30001 gtccatctcc ggggagaagt ggggagagtt gcggatgtcc ggcgccgtga tcgtctcccc
    30061 ccgggcggcg acgtagccgg cgatgccctg cccgagcttg atgcggaaat gcttcagggg
    30121 cgcctcgtcg agattgaacg ccaccttgaa cttcagttca ttgccgtcga gcaggagcag
    30181 ggagcccgcc tcgacgttca tggtgacgcg gatcatgtcc atcgtgtagt tgagaacctt
    30241 gtccatctcg aaggtcgaag aggacagggc gctgccgatc tgccggaggg tggcgagttc
    30301 ctggttgcgc cgcatgatgg aggcgagcac gttttccttt tcgacggcga gcgagagccc
    30361 ctccgtcatc atcgacagga ggcgcccgcg cttgtcgacc gcgtcgcgcg tcacgttgta
    30421 cagcaaaaga acacccgtga tccggccctc caccttcagg ggggccagca gacacgtccc
    30481 gtttgtcccc tgggggcaca gcgccttctc caggggaggg gccagggcgc tcaggtcgtc
    30541 caggaagagg gcgctctgct gccggatgac gcggtcgagc atcgacccct cgccgtcaaa
    30601 ggccgccccc cgggtcaggg cggtcgggac gaaggcgagg acatccagga cggagacctt
    30661 ccccggcgcg cttcggtccc agtccaggag gacggcgatg tcgtagggga tgtgccggcg
    30721 gagctcctgg aggagggtgt gaaacagctc cgtgccccgg agggtcgagt tgacggtccg
    30781 ggcgaagagt tccgtggatt ccatggcccg gtcccgttcc cggccggccg cgagcaggcg
    30841 gaggttgtcg aacgtaaagg aggcgctgct gacgagcggg gtgaggatgt cgacgtcggc
    30901 ctgcgtgaag gggatgtcct cccgcttgcg cgagaggctc aggacgccga tgacctcctg
    30961 gaggctcttg agcggcatgc agatgaagga ccgggtggcg tactgggagc gcgaggagcg
    31021 gccgaagcgg gtgtccgttt cgatattctc gaccagaagc ggcgatttgt tgatgacggc
    31081 gtacttggcg atgctcgttt cgaagggaat ccggtcgtcc atgttgacat gctcccccag
    31141 gccgatggag gcctggatgt ggaaggcgtc cctctggggc gacaggatca ggaccgaacc
    31201 gatatccgcc cgcgccagct tcagggcccg ctcgagggtg atgtacagga ggtcgtcggg
    31261 attgaaggtg atgtagcaca gctccgagag ctccttgagg gtggagattt ccgccgcctt
    31321 cttttcgaga tggcccaggt tcttgcgcag ctccccctcc agcgtccgga agctgtggaa
    31381 gatgccgttc aactcgtcgg tcgtcgttgc ccggttgccg gccagctcgg gggcgaggga
    31441 ctgggaaatg tcccgggaga ggttcaggat ctggtcgaaa acccggcgca ggatcacaaa
    31501 gccgaggaaa aaggcgatga gcaggacaat gaagaacagg gggagatagc ggtcgccgag
    31561 gatgtcgtat ttgacggcga agtagaggaa acccaggacc ggcgccacga aaaaaagagc
    31621 gaaaatcagg gagagtttat aatagaggcc cttgtttttg gtgaagaagc ggtccagccg
    31681 gatcctgaat cccatgaaac gattttcctc ccgtcggggg tgcgcaacgg gacgcattgt
    31741 aatgaccggc gccggggatt gcaaccctta ttctttcagg cctgctttcg tgcgtgcaaa
    31801 aaaaaggcac tgaagacagt gccttggaat ctttgggatg cgggatggac cgcgtcatgc
    31861 cgtggcggcg tacacgctga ccttcttgcg ccgcttgtcc aggcgctcga actttacgac
    31921 gccgtcgatc ttggcgaaga tcgtgtagtc cttgcccaag cccacgttga cgcccgggtg
    31981 gatccgcgtg cccaactggc ggatgatgat tccgcccgcc cggatggact gtcccccgaa
    32041 caccttcacg ccgcgccgct gggcgttgct gtcccggccg ttgcgggagc tgccctgtcc
    32101 tttcttgtgt gccatgacga cctccgctta gaacgagatt tccttgatcc gcatttcggt
    32161 gagctgctgg cggtgccccg tcttcttgtg gtatcccttg cggcgtttct tcttgaagac
    32221 ggcgatcttg gggcctttca tctgcgacac gatctccccg gttacccggg cgccggccac
    32281 gcgtggggcg cccacgcgga tgtcctcgtc tcgggcgacc aggaggacgt cctcaaagac
    32341 gaccgtatcg ccctggctcc cctcgagctt ctcaaccctc agcacctcac cctcgacgac
    32401 ccggtgctgc tttccgcctg tcttgatcac cgcatacatg attgtcatcc ttgccgaaaa
    32461 tttagaacgg catccttaca aaaaaacggg gccgtttgtc aacataaatc ttgagccggg
    32521 cgcgccgatt cccttccccc ttcttttccg aggacggcgg tcttgacgat gcgcgcctcc
    32581 cgggcggttg cctccggcag tttgaggacg tggatgagga gttcggacgg ccggacggcg
    32641 ccccgcgcgt cgaaccggac cgaggccgtc agccgccgcg cctccggcgc ccacttcagg
    32701 tcggcgacgt aggggcggat atcccgggtc tgcgtttttc ccttgccctc gcggacgaag
    32761 gggcaggtgt ccgccgcgag aaaggcggag acgcgctccg ggatcgcgcg tccgtccgtt
    32821 tcgtccaggt cgtcgggcag ggtgatctcg tagcggaagc cgccgatcgc gtcggccagc
    32881 gacggggtgg cggggtcgat cggcgccgcg tccgtcacgg cgatgcccgc cggcaggcgt
    32941 cggttgatgg cgtcgcgcag gcgctccggg tcgagccccg gatcgatcag ctcgatgtcg
    33001 gcgtattcct cgcggctctc cattcccacc gacgtggcca cggcgaagga caggcgcggg
    33061 tgggggtgga agccctcgct gtagcggaag tcgagccccg ctaggcggat ggcccggatc
    33121 aggatggcgg ccagctccag gtgggacagg aagcgcgccc ggccctcctt gacgaaccgg
    33181 aggcggtagc ggcgatagac gggcggacgg cttggcgccg gcgttccggg ttgggacggg
    33241 gcggcggtct cgggcggcgg ccccgcgggg acgatccgga tcgtttcgtg gtcgcagacg
    33301 ccgcatcgat ggcaggggcc cgtccggcag tcctcggtaa gtcgtccgtc gagcgcccgc
    33361 gccgcctctt ctcgcaggaa atcggcgctg acgccggttg cgatgcggtc ccagggcaac
    33421 gcggccgcgg gggggcgcgc cgccagggcg gcctcccggt cgacgccgag gccgtcgagc
    33481 gccgcctccc agaggtcgaa ccggaagcgg tcgccccagc cgtcgaagcg gcagcccaga
    33541 atgtaggccc gctccacgag gtctcccatc cgctcgtccc cccgggagag cagcccctcg
    33601 aggaggctca tccgtccgtc gtgccagcgg acggtcaggc ggggatggcg gaggcgcctg
    33661 cggaaatacg cctgacgcgc ctccgtttcc gcgaggtcga tctggcgctc ccactggaag
    33721 ggcgtgtggg gcttggggac gaaggtcgag aggctgacgg cgacctgtcc gggcgacttt
    33781 ccgcgcggca gggtcttgtg ggcgaggtcg acgatgccgt cgaggtccgt ctcgccctcg
    33841 ccgggcaggc cgatcatgaa gtagagcttg agcgagcgcc agcccgcgtc gaagacgttg
    33901 ccggcggtcg ccatcaggtc ggcctccgtg ttccccttgt tgatccggtc ccgcaggcgc
    33961 tgggttcccg cctccggggc gagggtgaag cttgtcttcc ggacgcggcg gatgacctcc
    34021 atgagccgcg gcgtcagggt ctccacgcgc agggagggga gcgccagggc gacgcggcgg
    34081 gcgtagtgcc ggtccatgag caggccgagg agggcctcga tgtgggggta gtcgccggcg
    34141 ctcagggaca ggagcgagag ctcctcgtgc cccgtggcgg cgagcatccg gtccgacatc
    34201 tgcaggagaa tgtccggatc gcgctcccgg acgggccgcc agagcatgcc ggcctggcag
    34261 aagcggcatc cccgggtgca gccccgggcg atttccagga ccagacggtc gtggatggtc
    34321 ttcaccaacg ggacgacggg gcagacgggt ccgtgccagg cgttcaggtc cgggacggtc
    34381 cgcttgcgga tgatctcccc gtgcgtgtgg agagcggggc agtagacccc ctcgatctcc
    34441 gccaggcgcg cgagccgcga ggcccggcgc tcgccccgcg acttcgcggc catcagggcc
    34501 cgggcgattt cgccgatcac ggcctccccc tcgccgatca cgaaggcgtc gatgaagggc
    34561 gacatgggca gggggttgaa ggcgcagggg ccgccggcga tgacgagggg gtggtcctcc
    34621 ccgcgcgccg ccgcgcggag ggggatgccg cccaggtcca gcatggcgag gacgttcgtg
    34681 taggacagct cgtactggag ggagaagccg acgaggtcga aggcgtgaag cggcgtgcgc
    34741 gactccagcg tgacaagcgg cagggcgcgg cggacgagct cgccctccag gtccggccag
    34801 ggggcgtagc accgctccgc cacgacgccg ggctccgcgt tgaggatgcc gtagaggatc
    34861 tggaggccga gatgggacat gccgacctcg tagacgtcag gaaaggccag cgcgatgctc
    34921 aggcggcagg ccgcatggtc cttgacgacg gcgttgactt cgccgcccag gtagcggccg
    34981 ggcttttcga cccgggtgag gaaggcctct tctctcattg gagccggttc cggaacgtgt
    35041 gggggttgtt caggtattcc cggtagcggg ccagggcgag gccgcgggcg aagtccggac
    35101 ggccgaggta gggcagggtg tgggcccgga aggccgcgag cagttcggcg aaaaccgcct
    35161 cccgcgcgtc gttgccgacg aaggtgcgct cgcggacgta ctcgtagacg atcggatcgt
    35221 cccagatccg ccggacgacg tcgaaggcct cgtcgcccgg gaagaaatcc cgttcgaggc
    35281 ggaccgggac gcgcacgtac agctcgacct tcgtcgtgtc cgcggcgatc ggccgcgacc
    35341 ggtcccagac ctccatcgtc aggccattgt ccagaacggt ggtttccacc agcgtcttca
    35401 tgatgccccc atcgggaacg gcgacccctc cgccggcggt tttcccgccc cggcgttccc
    35461 attccccgtc gcgtggcgtt gtctttacca tctttgtcct tgacctgcaa cgggagacat
    35521 tttataggcg gtgggaaaaa atagggacag cgccctataa atagggacag cgccctattt
    35581 tccaacagga gacaatttat aggcactccc ggcgagagag atcctttgaa aatagggaca
    35641 gcgccctatt ttccgagggc cggcgcctgt gagggtggat ccgttgaata cgggagcaag
    35701 cgatttcatc catctgtgcc agccggacgg cggcaagtcc tgcggggcgt gctgcg
//
LOCUS       DAOOLJ010000018        31044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_1243_length_31044_cov_17.335861, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000018 DAOOLJ010000000
VERSION     DAOOLJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 31044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31044
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1243_length_31044_cov_17.335861"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLW16_05075"
     CDS             complement(<1..901)
                     /locus_tag="PLW16_05075"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPB70312.1"
                     /translation="MKKEEYMWDYRNPYEWMTRTMTAKLPPMMITCAITGGVQGKEMN
                     ENIPETAEEQADQVYEAYKAGAVSVHIHARMPEALSMTSAAAEDYARVNKMVRERCPD
                     IIINNTTGGGPWLTTEQRMCCLFAEEPPDMASLNLGPFMLKVPLKDRKPPLKHPRDGY
                     MFDAIIPATYADINLYAKTMKEKGIKPEVEIYHQGQYWVLQDLIREGNIDPPYVIQFV
                     MGFQTSTYPTPANVLSFINELPPQSMFAVIGVGPFQIPMNAMGIMLGGHVRVGMEDNL
                     YFKKGEKVKNNAQLVTRVKQMAEI"
     gene            1330..1680
                     /locus_tag="PLW16_05080"
     CDS             1330..1680
                     /locus_tag="PLW16_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70313.1"
                     /translation="MSTPQAVKITDIKLNADSLYREESFTDLTYATIRRLTPVKIDGS
                     PDENRETLFTGMAQLMSPSGPIPVQCIIEGAKTLAEAAARLPDAIEAAVKAMIAEAKE
                     MERQEASRIVLPGQ"
     gene            complement(1762..3447)
                     /locus_tag="PLW16_05085"
     CDS             complement(1762..3447)
                     /locus_tag="PLW16_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB70314.1"
                     /translation="MATNPRPASRGRTRRLEESLALKNSLLTHIGEITALLTEQPDLD
                     RVFDKILVRVMTGLRFDRAVIMLLSDDGARLECKCIKGFTPEGERLAWANPFYLHRDD
                     CYETRVIRTGQPVVLQDTSGDPTETDMDRLINRHHIRQGQERKSLIYVPLKFKGKILG
                     FIGGDRFRTRMEITPDEVEAMVIFANHASIVIENTRLDKERRDKQSLLEGVIKSSING
                     IIVSDIRGNILHINPKAEEMAGITQAEARGILAQELSDLDEEERQRLYQVFKNKENIS
                     HYERMHTRRDGQRLVVSLSCFPVIDENDRTLGVVTEFTDVTEKKRMDDYYVRLEKFAA
                     LGHIASAIAHEIRNPLAGIAATVQNLEVDCEEGSPQKTDLRNIIHEVDRLEKLLRDIL
                     GLARPLPLQMEELKVRDLLDSTLSLVKKEASKRAIAIRTAFPHPEILIRADAGRLRQV
                     FLNLTLNAMEAIDTTGEIAVTTATQADEKGRPERLVISFRDDGPGIPPGHLNKIFDPF
                     FTTKKVGTGLGLAVCQRIIQDHDGMIEVESRENAGATFRITLPVLTGALEGRG"
     gene            complement(3498..4328)
                     /locus_tag="PLW16_05090"
     CDS             complement(3498..4328)
                     /locus_tag="PLW16_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB70315.1"
                     /translation="MRSDFWRRFLENRMAVAGSVVVLALFVVSLFAPWLAPYDPSAID
                     LDRVLMPPSGDHWLGTDPLGRDVLSRMIWGARISLKVGFIATGIAILIGAVLGAMAGY
                     YGGWTDALIMRFVDIMLCFPAFFLILAVIAVLEPSIWNIMLIIGVTGWMGITRLVRAD
                     FISLKERDFVLAARVIGASDLRIIFVHILPNAMASILVAATLGVAGAILTESALSFLG
                     IGVQPPTPSWGNILTAGKDNIDIAWWLSLYPGLAILVTVLGYNLLGEGLRDALDPRLR
                     "
     gene            complement(4339..5313)
                     /locus_tag="PLW16_05095"
     CDS             complement(4339..5313)
                     /locus_tag="PLW16_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB70316.1"
                     /translation="MLIYLGKRLLFMAPMLVGITIICFAVMHLAPGSPTDLQTQMNPR
                     ASAEAKERLRALYDLDKPLTQQYWLWVKKLAVLDLGRSFSPDRRPVSDKILERLPITI
                     TLNVLSLLLIVAIAIPIGVLAAVHQDAWFDRATSVFVFIGFAMPTFWLALLLMILFGI
                     HLGWLPISGIRSLNAEYMRPAAAFWDLIRHLILPVLLSAFGGLAGLSRYMRANMLEVI
                     RQDYIMTARAKGLSERTVIYRHALRNALLPVITILGLSVPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPVVMGILFIGAVLTLLGNLIADVSYALADPRIRVA"
     gene            complement(5307..6119)
                     /locus_tag="PLW16_05100"
     CDS             complement(5307..6119)
                     /locus_tag="PLW16_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPB70317.1"
                     /translation="MTSSVLKHVQVHVPFPMLKEKLLSMVVRERVHPEIGFSHVALDG
                     YSRADFAAVAERLVGEGLPVTLHAPFVDLRPGALDPEIRRVTAARLQQVFDLAPLFRP
                     RSIVCHPSFDIRYYVTAERLWLEHSVETWRAFLPQAEQMGTIIALENVYERDPQELER
                     LLAALPSERVCCCFDTGHFNAFATTPLDRWIVSLGDRIGQLHIHDNHGRMDEHLPVGE
                     GTFPFAEFFRRLRERGRRPIITIEAHSEKNLWRAVKNLEDMNLLAILETDPC"
     gene            6266..6664
                     /gene="rsfS"
                     /locus_tag="PLW16_05105"
     CDS             6266..6664
                     /gene="rsfS"
                     /locus_tag="PLW16_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPB70318.1"
                     /translation="MTVKTDKVHSAVLTCLNAILEHKARNLVLLNVQNLSSIADYFIL
                     CSGASDRQVQAIAAFVQETLKKKSGLLPLGVEGESVAQWVLIDYGDFILHIFYEPIRE
                     FYGLERLWADAPRMEIGDDVLKVKALAPEM"
     gene            6667..7383
                     /locus_tag="PLW16_05110"
     CDS             6667..7383
                     /locus_tag="PLW16_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPB70319.1"
                     /translation="MRRLLAGLLDLIFPPRCAGCGRVLGDDGLPPFCPACQAEVRLLQ
                     SPLCTRCGAPFDGGDADHLCGACLADPPGFTLARSVGRYESVLLDAIHRFKYGGDTAL
                     GRALGRFMTRHPCPGMEIDADTRIIPVPLHVNRLRERGFNQALILARELARPGGQTVD
                     FLSLKRRHATDPQVRLGKNARRRNIRGAFVVTRPERIADRRILLVDDVYTTGSTVGEC
                     ARVLMAHQAAEVAVLTLARA"
     gene            7552..8034
                     /gene="rfaE2"
                     /locus_tag="PLW16_05115"
     CDS             7552..8034
                     /gene="rfaE2"
                     /locus_tag="PLW16_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPB70320.1"
                     /translation="MGKILSREALGEAVDRLHRGGSKIAFTNGCFDILHVGHVQYLEA
                     ARREADVLIVAVNSDASVRAIKGEKRPLVPEGERAAVVAGLAAVDFVTLFSEQTPLDL
                     IQSLRPDVIVKGGDWKEEDVVGRDAVRAWGGRVMIIPETAGASTTNLVAKILETYGRP
                     "
     gene            8060..9319
                     /locus_tag="PLW16_05120"
     CDS             8060..9319
                     /locus_tag="PLW16_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70321.1"
                     /translation="MNSLPPFDSAATAMGQPWQRTLYIMFAAQFLSIIGFAFALPFIP
                     FYIRELGVTDERLVPVWAGIITAASSLVMAFFSPLWGWLADRYGRKIMVERAMFGGAV
                     LTFAMGLVTDVWQLLFLRLLSGATTGTIAASIALVSTVVPPKRLGYSLGIMQVAVFLG
                     MTLGPWIGGLLADAVGYRVTFMTGGGILFLGGSLVLLGARERFNRPEKGTLGGGDRLF
                     SLLAYAGFPALMALFFFFNFSLHFVMPILPLFIETMCDPSRGVASTTGLLFAISGGAA
                     ALSAGGIGALSDRMGYKPILVTMLLLTSTSLLLHGLAQGIAHLVLLRVLYGIAAGGIL
                     PTMNALVGRLIPPSGYGKAYGLTSATTCLGMAAGPFFGGLMASGWGYRWPFVFISAIM
                     AAVTLLVILGLRRRSQGRPPIPGENKT"
     gene            9510..9710
                     /locus_tag="PLW16_05125"
     CDS             9510..9710
                     /locus_tag="PLW16_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB70322.1"
                     /translation="MAEGTVKWFNEQKGFGFIENAEGGDVFVHYSAIQAGGFKTLAEG
                     QRVSFDVTQGKKGPAAENVKPL"
     gene            9802..10005
                     /locus_tag="PLW16_05130"
     CDS             9802..10005
                     /locus_tag="PLW16_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70323.1"
                     /translation="MARTKYTFEKRQKELAKRRKKEEKAARRAESRRPAENETGAGTA
                     GEAPDSAAEASNTGADAPTPDPA"
     gene            complement(10002..11483)
                     /locus_tag="PLW16_05135"
     CDS             complement(10002..11483)
                     /locus_tag="PLW16_05135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB70324.1"
                     /translation="MDGLIEPENALPEITLEGLPEALQAACTRTGWTKLLPVQARAIP
                     YVLAERDLMVQSQTGSGKTGAFILPILERIDPAKAVCQALVLVPTRELAKQVAHEAET
                     LQGERGLRTAVVYGGVKYEPQLEAFRRGAHLVVGTPGRILDHLLKGTLSLDTLKIIVF
                     DEADRMLSMGFYPDMKRVKQFLPRRPISGYMFSATFPSYVLRLAGEFLRHPEFLSLSQ
                     EHVHVTGTSHIFYIVPGMQRERSLVRIIEMENPVSAIIFCNMKSTVHFVSVVLKRFGY
                     DADELSSDLAQGARERVMGRVRRGALRFLVATDVAARGIDIPDLSHVFQYEPPEDPEL
                     YIHRAGRTGRVGATGTAITLVAGMEQFNLRSIARSFNINIQEKTLPTDEDVEAIVSQR
                     VIASLEANLRSRDSLERERMRRFIHLGRSLAEAEVESPLIAMLLDDYYQRMLQAPPQT
                     LVAEGDGEGISETEAAPAAEEKKLPRRREGQRRRGPRRPRPGR"
     gene            11917..13314
                     /locus_tag="PLW16_05140"
     CDS             11917..13314
                     /locus_tag="PLW16_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPB70325.1"
                     /translation="MIAQEPRLAVGIISRATGISGVLNGVFRADPEHVPAAERTVRLQ
                     GPFGVFLDGGKPLLRTGSGEIRPLGRTLSLVPEAEADFTLNDVTIGVSFHWERREAQT
                     FPGGLTLLSPDDGTLTAINVVPLETYLASVISSEMSALAPPEFLKAHAVTSRSWLAAM
                     LARRTDPGTAARPESEPGGASRTSTLPAAEGRELPEIRRWTGREAHAHFDVCADDHCQ
                     RYQGMTKIVSPAAAAAVSVTRGVFLTHAGRICDARYHKACGGLTEPAQNVWAEVPIPY
                     LTSVADADGTLPPVRTEAEAAAWFSDRPDVHCRVTDPGLLARILPDFDRETADFFRWS
                     VSYEREELEGLLKGASGIDFGTLLDLMPLTRGPSGRISRLRVTGTKHAVTVGRELEIR
                     RWLSKSHLFSSAFVVRTERDHSGIPMRFHLAGGGWGHGVGLCQIGAAAMAARGRTAEQ
                     ILAHYFPGATVTRRY"
     gene            13320..14042
                     /locus_tag="PLW16_05145"
     CDS             13320..14042
                     /locus_tag="PLW16_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPB70326.1"
                     /translation="MGTPPVKTAFILGAGLGTRLKPLTDRCPKPLLPVGGQPLITHVM
                     DHLLSTGVERFIVNTHHRAEAYDRAFPDKTWRGRPILFRHEPVLLDTGGGLKNITDLL
                     AGDDRLLVYNGDILTDLPLDPLLAAHRRRDAEVTLALRSAGKPLNVTIDGAGNVRDMR
                     DLLGDPGIRRCLFAGIYVMETRFLEHLPGGPIFSIVPALVDRIRRRPGTVAGRVIDAG
                     RWHDIGSPAAYDALRNAWSPAP"
     gene            14039..15049
                     /locus_tag="PLW16_05150"
     CDS             14039..15049
                     /locus_tag="PLW16_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPB70327.1"
                     /translation="MSAPLPSDAALLGFARRALNLADAPPPDLAPITRGGSDRVYRRL
                     TAGGSSWIVMQYGTDRRENASFAPIARFLAARDIRVPVILAEDAARGLLVLEDLGDRD
                     LWSYRDADPAVRLSLYRRTLRLVHRLHGLAAGDAAVRALPLMDPFDAAFYRWERDYFR
                     EHFVRGVCALALDPSEAAGLEAELAALAARLGGAAPCLVHRDLQSQNVMIRDGEPVLI
                     DFQGMRLGSRLYDLGALLYDPYVRLTPDVRLDLLRDYYDLSPRALSWNAFREAFQEAS
                     VQRLLQALGAYGYLGLKAGQPDFLAHMPRGLDHLEAAAGLAPRLDHLRRLLSRCRRAI
                     NR"
     gene            15081..16790
                     /locus_tag="PLW16_05155"
     CDS             15081..16790
                     /locus_tag="PLW16_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB70328.1"
                     /translation="MEIQPRKSRPGITAALSYADAPWFDALLRRLIRQSAIERILIAY
                     NDGDAYPHDKCEWIPAGSLVAGETLSTVIEKTQTKYLLILPFCADLELGPRALRRLLE
                     AAEICQAGMLYADYLEGHTPSPAAPAGNGVPPARKRGKKDANPALREHPVNDYQPGSL
                     RDDFDFGPLMLFSMDAIRTALKRYGALSPFTHAGLYDLRLRVATDATLFHLRESLATV
                     IRPEPAATDAPAADECEAWTDEGETNGGDPASAPFAYCDPRQQDRQREMESAATTHLK
                     RLGAWLRPRFRSVVPTSQAFPVEASVVIPVRNRKKTIADAVRSALSQEADFPFNVLCI
                     DNHSTDGTTAVLEKLAKADPRVIPLVPARLDLGIGGCWNEALRAEACGRFAVQLDSDD
                     VYSGPDTLRKIVQCFREGDVAMVIGAYTIVDERGNILPPGLIAHREWTDDNGRNNALR
                     INGLGAPRAFQTALARQIGFLNVSYGEDYAIALRLTRDYRIGRIYENLYLCRRWSGNS
                     DSALTVAQINRHDAFKDTLRTLEIMARRQMNPRPAVPVPGREGGRGYPGYGSPACRSG
                     APS"
     gene            16787..17314
                     /locus_tag="PLW16_05160"
     CDS             16787..17314
                     /locus_tag="PLW16_05160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB70329.1"
                     /translation="MTFSVQPWQKHDLRAVREITWTTWQSAYAPFIPADNLKTYFGAH
                     YSLRALRAVYADPFVDGFIGTTECGPAGYLRTRYVGDEHRFYVTSLYILPEHQGRGLG
                     GKLLAAAEARALRYGVDAVWLGVMARNKRALAWYRRRGFRFIRKEPFALAATTVEHRI
                     GYRKMNAAAARVPPP"
     gene            17311..18327
                     /locus_tag="PLW16_05165"
     CDS             17311..18327
                     /locus_tag="PLW16_05165"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPB70330.1"
                     /translation="MTRPVRQTRRVPGDRIAAVFEGSHGPRPAGGLSPLCRALYADQT
                     RSWPALARAEAELAGIRVRDLSSDGWSLKVQYNPARLKSGTARVDPASIAARPCFLCP
                     AALPPEQRGILYREEYLILCNPAPIFPAHFTIASLAHEPQAIEARLEAFLDLARDLGD
                     GMTLFYNGPACGASAPDHLHFQAAPAGAIPIEKDLAGRFDARPAWGNNGVDRWGLVSW
                     GRTLILIRGRERTPLAHAMRRLIAGGGTSPGSPDEAMLNLLAGHRRGEWRLCLFPRRR
                     HRPAAYDRPDPERLLVSPGAVDMGGLIVAPREREFTRLNAPMLRNIYQEVSSPPRADL
                     LARE"
     gene            18383..18949
                     /locus_tag="PLW16_05170"
     CDS             18383..18949
                     /locus_tag="PLW16_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB70331.1"
                     /translation="MTEPSRPVSPLVDSAMSFPEAVAGAQAPPEALAPLSLVDVRYWA
                     FDGRLHAGQLVVHRDLAADVRAIFGWMQRSRFPVAKVVPIVRYGWSDDASMADNNSSA
                     FNYRFVDGTERLSLHALGRAVDLNPRINPVIYGDGRIAPPGAAYRPGAPGTVYPAHPL
                     MEEFRKRGWTWGGDFKTLKDYHHFEKRG"
     gene            complement(18988..19440)
                     /locus_tag="PLW16_05175"
     CDS             complement(18988..19440)
                     /locus_tag="PLW16_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPB70332.1"
                     /translation="MSRTSSMRGLALLAVVGPLMLLSAAPPLRAQESGAAEADRMAPA
                     ESATVGEPEEGSVCRAFYYAKRFNGRRTSSGAIYRPERRTAAHPSLPLGTRVRVVNIA
                     NDRSVVVTVNDRCRKRSFDVIDLSRAAARELRFLRGGTAWVRVIPLDD"
     gene            19667..21121
                     /locus_tag="PLW16_05180"
     CDS             19667..21121
                     /locus_tag="PLW16_05180"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HPB70333.1"
                     /translation="MKTPQEYIDSLRTLDLKVYLLGKRVENVVDDPIIRPSLNAVAMT
                     YALAQDPGYADLMTATSHLTGRKINRFTHIHQSVEDLVRKSKMGRLMGSRTGCCFQRC
                     VGMDALNALSMTTYDIDQRHGTEYNKRFLKYLEYVQENDLVCDGAMTDPKGDRSLPPH
                     RQADPDQYLRIVSESREGIVVRGAKAHQTGAVNSHEVIVMPTIALREEDRDYAVSFAL
                     PSDTAGITYILGRQSCDTRKLEGGRMDVGNVIFGGHEALIVFDDVFVPWERVFMCREY
                     GFAGQLVEQFASYHRQSYACKVGVGDVLIGAAQTAAEYNGAEKASHVKDKIIEMNHLN
                     ETLYCGCIACAAEGHREPSGTYLVDTLLANVHKQNVTRFPYEIARLAQDIAGGLMVTL
                     PSEADLNAPETGKWMQKYFRGRSDIPTEERMRILRLIENLTLGTAAVGYLTESMHGAG
                     SPQAQRIMIARLVDLKQKQAFAKALCGIRDAKPR"
     gene            21215..21412
                     /locus_tag="PLW16_05185"
     CDS             21215..21412
                     /locus_tag="PLW16_05185"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPB70334.1"
                     /translation="MDRNVGTIDRIVRLVIAIFFIAANAAGCVTGLAGLVLAVLAGML
                     LSSVASGHCPLYVRLGIKKSV"
     gene            21573..24788
                     /gene="recC"
                     /locus_tag="PLW16_05190"
     CDS             21573..24788
                     /gene="recC"
                     /locus_tag="PLW16_05190"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPB70335.1"
                     /translation="MPGLKLYHSNRMEVLADRLAEVLAVPLADPFAPEIVLVQSRGME
                     RWLSMRLADRHGVCANVRFPYPTHFVHALFRAVDPGLPARSPYEPAVMTWDLVDLLSA
                     LPDRPEYRPLKAYLDDDGNGLKRFQLAARVAALFDQYLLFRPDWMPAWERGQETHWQA
                     ALWREIVRRKGPVHRVALRRKLLAAIADATVPLPGAPERVAMFGISSLPPFYMQILSA
                     LAARIEVNLFLVNPCREFWEDIVSEREAGRIAERLAGYDRRPEAYHLEAGNRLLASLG
                     PLVREFLRTIAELSAEEFPQFVDPGDATLLAAIQSDILNLTERVPAGGDAPAADDGSL
                     RVHVCHSPLREAEILHDALLAMFDADRTLRPDEILVMIPDIAAYAPFIQAVFSAAADD
                     PRAIPFTLADRGYLHESHLIDTFLHLLDLAGSRYGAAKVLDLLDAPAVREKFSLADDD
                     VRLVRHWVRETGIRWGIDARSREALDLPAEAANTWRAGLDRMLLGYALPAGGEAVLVD
                     GIFPYDDIEGGAAEILGPFLAFTEALFTAIPALETGRTLSGWAQALTALLETFFSDDE
                     RYGAETQALRRLLGELPELEAASGYAGEIGIEAVKAFLRENLTDRGSGGGFLAGGVTF
                     CTLLPMRSIPFKVIALLGMNDDAYPRPSVPLGFDRMASAPRPGDRSRRQDDRYLFLEA
                     LLSARQRLHISYCGQSPKDNRPRPPSVLVSELLDAIEAGFGPRVREDLCTHHRLQAFD
                     PAYFSGDPKLYSYSTENLRGAARAAAPRREPGAFIPSGLPEPGAGLTEIDVETFCAFF
                     AHPARFLLRRRLGLYLRDREAAIPEEEPFTLEGLDAYGLGQDLVARLLADRDIAQAYP
                     VLKAAGRLPHGAPGAYAYDRLHDAMTGLAADIGRAVSGGRIEAVPVDLRLAGVHLTGA
                     LDPLYPEGLVRYRPADVKARDRLDAWIRHLILNHPGCAAPVRETRFIGRDRGVRYAPL
                     AGAEQPLADLVTLFLAGLRTPLHFFPESSLAYADATAAGKDAAAALRAARAKWEAFRY
                     PEKDDPDNELCFDRIDPLDDAFRRLAETVFHPLLAAEEALT"
     gene            24791..28429
                     /gene="recB"
                     /locus_tag="PLW16_05195"
     CDS             24791..28429
                     /gene="recB"
                     /locus_tag="PLW16_05195"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HPB70336.1"
                     /translation="MDAPEIFNLLESPLTETNLIEASAGTGKTYAVEGIFLRLLLERG
                     LSVKEILVVTYTVAATEELRDRIRGRVRAAVAAFTSGGHDDPFLDGLVRRTTAREEAL
                     RRLRAALRDFDEAPIHTIHGFCQRALREHAFETRGTFDAEMIPDDRALKEEIVRDFWR
                     RHLYGGAPEVPGHALARKFGPEGFLELIEGRLFDPGMRILPEAPPVVLDALPPFREAV
                     EAVRRAWPAARDAVAACLRDPGLKRNIYRNPDALLAEMDAYTAARRTWPPFDGFDRFT
                     ATAIEQGTRKGCTPPEHPFFAACETLRERAAAWTEQADRHLLFLKAELFRTVRAELRQ
                     RKRRRNIQCFDDLLLDLREALAGPGGSALAEKLRARYRAAVIDEFQDTDPIQYAIFET
                     VFGHGDATLFLIGDPKQSIYSFRGADLFTYLRASHDVRRRHTLRHNWRSEPALIEAVN
                     AVFRHPDGAPFLYDAIPFAEAIAGDVPERAVLTVDGRPSPPFRLWFLDGVRLEAAGAA
                     KNQDRAADLIIRSVAAEISRLLDPRKGPRTTIGDAPLVAGDIAVLVRTNREARRVREA
                     LRTLGIPAVLHSTGNLFDTREAIEIERVLGALAAPRDEGRIRIALATDLMGLNGEALH
                     ALSGDETAWEDRLARFRAYHALWDERGFIPMFRRFLAAENVRERLLAFPDGERRLTNV
                     LHLAEVLHAEAVSGRQGMAGLVQWLARQRDPVTPRLEEHELRLESDARAVNIVTIHKS
                     KGLEYPVVFCPFHWRGTKLRQGEFLFHDPADDWRPTLVLTSDAGTGRDLALRETLAED
                     IRLLYVALTRARHRCYLVWGPFKDAGASALAYILHPTAGETGDGTAATEAGATGLDGN
                     ALRRELEALAASVPGAIALEEMPPPDAAPRAASPEAETALTCRTFTGLPDRDRPIASF
                     SSLVADRALRPESPAEIGDPADLPDRDGEGGAAAPPPAGKPSGVFALPRGTKTGNLLH
                     EILEEIDFTAASGPAAERLFADKLAAYGFDPACRETVARTIRLALDTPLDPDLDGLTL
                     STVGRTDRLSELGFYVPLNRLTPDRLERLLAETGIAPTSGFPEGIGRLRFQPVRGFMR
                     GYMDLVFRRAGRVYLVDWKTNFLGDTVADYRPGALAQAMREAFYPLQYHLYVLALHRY
                     LKRRLPGYDYDTHFGGVFYLFLRGLDSGNSGATGIYRDRPARETIAALERALLPAAAG
                     SDGREEVP"
     gene            28426..30276
                     /gene="recD"
                     /locus_tag="PLW16_05200"
     CDS             28426..30276
                     /gene="recD"
                     /locus_tag="PLW16_05200"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPB70337.1"
                     /translation="MKNDAGSLPPRSRFSPLDEAFGRFIRRIDGRDAPELALAAAWLS
                     RSRAEGHICLDLGARPEDGFPVAGEGLPARPEAERWNALLETSPAVGRPGDRLPLILD
                     GTRLYLFRYWAYEQAVVRTLDTLAVDAAGVADEARLRAAFDCIFPPNGGAERIDWQKV
                     AAFAAARHRFCVVSGGPGTGKTSTVARILALLVAQDPDRPPAVALAAPTGKAAARLQE
                     SIREAKATLALPPALGAAIPETASTLHRLLQSRPETPYFRYHADRPLPADVVIVDEAS
                     MLDLALFAKLIGALRTRARLILLGDKDQLASVEAGAVLGDICDTGRAHGYSPAFADAC
                     LRVTGERIPADDVSAPGRLNDRIVSLRKSYRFGETSGIGALSRAVNAGDASRAGALIR
                     DAGTGDVAWRELPPPEALPRLLAAWIGTHDIAPPTAGDPAELLERFGRARMLCALREG
                     PYGVRHMNLLAERVLRETGRIGGGSLWYAGRPVMVTKNDYGLGLFNGDIGIALADPEH
                     RGDLRVFFPAGGGRVRSFPPLRLPEHESVYAMTVHKSQGSEFDDVLFLMPDRDVPVLT
                     RQLVYTAVTRARRTLWIAGREELFAAALSRHLKRASGLRDALWGSGTTCA"
     gene            30411..>31044
                     /locus_tag="PLW16_05205"
     CDS             30411..>31044
                     /locus_tag="PLW16_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70338.1"
                     /translation="MTHFPGQHLPEHQRGLLHRFYAISFLTILIPVLVVSYLIYSTGL
                     SLDDNHLLMFAMTLLLMLAGLVILRYVFTGIMAVVDILKQRDPEDGSLPLVIREDVSE
                     LKEISACLANLMNRFEKTTGELDRRVFELNTVNEMSEITRKTLRMDELLKVVLEKAMA
                     VVGAQTGSIFTVDGDAKPPRLVASSPPGAVPNGTSVNLERSFMKRVIEDRK"
BASE COUNT         5042 a        10801 c        10082 g         5119 t
ORIGIN      
        1 tgatctccgc catctgcttc acccgcgtca cgagctgggc gttgttcttg accttttcgc
       61 ccttcttgaa atacaggttg tcctccatcc ccacgcggac gtgaccgccc agcatgattc
      121 ccatcgcgtt catcgggatc tggaacggtc ccacgccgat caccgcaaac atggactgcg
      181 gcggcagttc gttgatgaac gaaaggacgt tcgccggcgt cggatacgtc gaggtctgga
      241 accccatgac aaactggatc acatagggcg ggtcgatgtt cccctcccgg atcagatcct
      301 ggagcaccca gtactggccc tggtggtaga tctcgacctc cggcttgatc cccttctcct
      361 tcatcgtctt cgcatacagg ttgatgtcgg cgtacgtcgc gggaatgatg gcgtcgaaca
      421 tatagccgtc gcgggggtgt ttgaggggcg gcttgcggtc cttcaggggg accttgagca
      481 tgaacgggcc caggttcagg ctcgccatgt ccgggggctc ttctgcgaag aggcagcaca
      541 tccgctgctc cgtcgtcagc caggggccgc caccggtcgt gttgttgatg atgatatccg
      601 ggcagcgctc gcggaccatc ttgttgaccc gcgcataatc ctccgcggcg gccgaggtca
      661 tggacagggc ttccggcatc cgggcatgga tgtggacgga gacggccccc gccttgtagg
      721 cctcgtacac ctggtcggcc tgctcctccg ccgtctcggg gatattctcg ttcatctcct
      781 tgccctggac cccgcccgtg atcgcgcagg tgatcatcat cggcggcagc ttcgccgtca
      841 tcgtcctcgt catccactcg tacggattgc ggtaatccca catgtactct tccttcttca
      901 tcgaaatgtc ctcctttcct gaatcatcct gcatgcaaat ataccgtctg cccggttgca
      961 ccctccaccg tttctccaac cggcccgtcc agttctgaag gcaggagcaa agggtatgcc
     1021 gccccgcgcc ggaatgcctc ccggggacgt atgaatcttc aatgcgcttt tattctggtc
     1081 gattacgatg cggacccgtc gggcgccccg ggggccatgg gcgcgctccg gagagaatac
     1141 agggggaaat gtggatgatt tcggatcagc gcgtggacgg tttcaatcca cccgatcccg
     1201 atggggaagg cgtccaaagc ggcgctttgt caaatatcgt ttgaccgctc acccttatcc
     1261 gatgtacgga ggctgcgaac aggttccggg ccggatgaac ggccccctct gaagaaaagg
     1321 agacaatcga tgagcactcc tcaagcagtt aaaatcacgg acattaaact caatgccgac
     1381 agcctttaca gggaggagtc cttcacggac ctaacctatg cgacgatccg gcgtctcacc
     1441 cccgtcaaga ttgacggttc gcccgacgag aaccgggaga ccctttttac cgggatggcc
     1501 cagctcatgt ccccgagcgg accgattccg gttcaatgca tcatcgaagg ggcgaagacg
     1561 ctcgccgagg cggcggccag gctacccgac gccatcgagg ccgcggtcaa agccatgatt
     1621 gccgaggcca aggaaatgga gcgtcaggaa gcctcccgaa tcgtcctccc cggacagtag
     1681 acagtgccgg tccggcaggg cctgaccatt gccggacgtg taaacccgtg cgcggcagga
     1741 cggaaaagtc gtccctgccg atcagccccg cccttcaagc gctcccgtca gaaccggcag
     1801 ggtgatgcgg aaggtggcgc cggcgttctc gcggctctcc acctcgatca tgccgtcgtg
     1861 gtcctggatg atgcgctgac agacggcgag gcccaggccg gtgccgacct tcttcgtcgt
     1921 gaagaagggg tcgaagatct tgttcaggtg tccggggggg atgccgggac cgtcatccct
     1981 gaagctgatg acgagccgtt cgggccggcc cttctcgtcg gcctgcgtcg ccgtggtgac
     2041 ggcgatttcg cccgtcgtgt cgatggcctc catggcgttc agggtcaggt tcaggaacac
     2101 ctgccgcaag cgccccgcat ccgcccggat gaggatctcc ggatggggaa acgccgtgcg
     2161 gatcgcgatg gcccgtttgg acgcctcctt tttgaccaga ctcagggtcg aatcgaggag
     2221 atcgcggacc ttgagctctt ccatctgcag gggcagcggg cgggcgaggc cgaggatgtc
     2281 gcgcaggagc ttttccaggc ggtccacctc gtggatgatg ttccggagat cggttttctg
     2341 ggggctcccc tcctcacagt ccacctccag gttctgaacg gtggcggcga tgcccgccag
     2401 cgggttgcgg atttcgtgcg cgatggcgga ggcgatgtgc ccgagggcgg cgaatttctc
     2461 caggcggacg taatagtcgt ccatgcgctt cttttccgtg acgtcggtga actccgtcac
     2521 cacgcccagc gtccggtcgt tctcgtcgat aaccggaaag caactcaggg agacgacgag
     2581 gcgctgcccg tcgcggcggg tgtgcatgcg ctcgtagtgg ctgatgttct ccttgttctt
     2641 gaagacctgg taaaggcgct gtcgctcctc ctcgtccagg tccgaaagct cctgggcgag
     2701 aatgccccgc gcctccgcct gcgtgatgcc ggccatctcc tccgccttgg gattgatatg
     2761 caggatgttg ccccggatgt cgctgacgat gatcccgttg atggaggatt tgatcacccc
     2821 ttccaggagg ctctgcttgt cgcgccgctc cttgtccagc cgggtgttct caatgacgat
     2881 cgaggcgtga ttggcgaaga tcaccatggc ctcgacctcg tccggggtga tctccattcg
     2941 ggtgcggaag cggtcgccgc cgatgaatcc taggatcttg cctttgaact tgagcggcac
     3001 gtagatcagg gatttccgct cctgcccctg acggatgtga tgccggttga tcagccggtc
     3061 catgtccgtc tccgtcggat cgccggaggt gtcctggaga acgacgggct ggccggtgcg
     3121 gatcacccga gtttcgtagc agtcgtcccg gtgcaggtaa aaggggttcg cccaggccag
     3181 ccgctccccc tcgggggtga accccttgat gcacttgcat tccagccggg cgccgtcgtc
     3241 gctcaggagc atgatgacgg cgcgatcgaa gcgcagcccc gtcatcaccc ggacgaggat
     3301 cttgtcgaag acgcggtcca gatcgggctg ctcggtgagg agcgcggtga tctcgccgat
     3361 gtgcgtcagc aggctgttct tgagcgccag gctctcttcc aggcggcgcg tccggccgcg
     3421 ggatgcgggg cgtggattgg ttgccatttc gaacctcttt cggaagcggg ggccgccgcg
     3481 tcgacggccg aaaaccgtca gcgaaggcgt ggatccaggg cgtcccgcag gccttcgccc
     3541 agcagattgt accccaggac ggttacgaga atggccagcc ccggatacag ggagagccac
     3601 caggcgatgt cgatattgtc cttgcccgcc gtgaggatgt tcccccaact cggcgtcggg
     3661 ggctgaacgc cgatcccgag gaaactcagg gccgactccg tgaggatcgc cccggcgacg
     3721 ccgagcgtgg cggccacaag gatggaggcc atcgcgttcg gcaggatgtg gacgaagatg
     3781 atcctgaggt cgctcgcccc gatcacccgg gcggcgagga cgaaatcccg ctccttgagc
     3841 gagatgaagt cggcgcgcac gaggcgcgtg atccccatcc agccggtgac gccgatgatc
     3901 agcatgatgt tccagatgga gggttcgagg acggcgatga ccgcgaggat caggaagaag
     3961 gcggggaaac acagcataat gtccacgaag cgcatgatga gggcgtcggt ccagccgccg
     4021 tagtaccccg ccatcgcccc cagaacggcc ccgatgagga tggcgatccc cgtggcgata
     4081 aacccgacct tgagcgagat gcgcgcgccc cagatcatcc gcgacagaac gtcgcggccg
     4141 agcgggtccg tccccagcca gtggtcgccc gagggcggca tcaggaccct gtcgagatcg
     4201 atggcgctcg ggtcgtaggg ggcgagccat ggggcgaaga gggagacgac gaagagggcg
     4261 agcacgacga cgctccccgc gacggccatc cggttctcca gaaatcttcg ccagaaatcc
     4321 gatctcatgg tctctccctc acgccacccg gatacgcggg tcggccaggg cgtaggagac
     4381 gtccgcgatc aggttgccca ggagcgtcag caccgccccg atgaacagga tccccatgac
     4441 gacgggatag tcacgcgcca tgaccgacat gtagaacagt tgccccatcc ccggaatggc
     4501 aaagatggtc tcgaagatga cgcttccgcc gatcagcccc gggacggaca gccccaggat
     4561 ggtgatcacc ggcaaaaggg cgttgcgcag ggcgtggcgg tagatcacgg tccgctcgga
     4621 aagccccttg gcccgcgccg tcatgatgta gtcctggcgg atcacctcca gcatgttcgc
     4681 ccgcatgtag cgcgacaggc cggccagtcc cccgaaggcg gacaggagca cggggaggat
     4741 caggtgccgg atcaggtccc agaaggcggc ggcgggccgc atgtattcgg cgttgagcga
     4801 gcggattccg gagatgggca gccaccccag gtggatgccg aacaggatca tcagcaggag
     4861 cgcgagccag aaggtcggca tggcgaatcc gatgaagacg aagacgctcg tcgcccggtc
     4921 gaaccaggcg tcctggtgca ccgccgccag cacgccgatg gggatggcga tcgcgacgat
     4981 cagcaggagc gacaggacgt tcagggtgat ggtgatcggc aggcgctcca ggatcttgtc
     5041 gctcacgggc cggcggtccg gcgagaagga gcggcccaaa tccaggacgg cgagcttctt
     5101 gacccacaac cagtactgct gggtgagcgg cttgtcgagg tcgtacaggg cccggaggcg
     5161 ctccttcgcc tcggcggagg cccgggggtt catctgggtc tggaggtcgg tcggcgatcc
     5221 gggcgccagg tgcatcacgg caaaacagat gatcgtgatg ccgacgagca tcggcgccat
     5281 gaacaggagc ctcttaccca gatagatcag cacggatccg tctccagaat cgccagcagg
     5341 ttcatgtcct ccagattctt caccgcccgc cagaggttct tctcggagtg ggcctcgatg
     5401 gtgatgatcg gtcgccgccc ccgttcccgg agcctccgga aaaattcggc aaagggaaag
     5461 gtcccctcgc cgaccggcag gtgctcgtcc atccggccgt ggttgtcgtg gatgtggagc
     5521 tgaccgatcc ggtcgcccag ggagacgatc caccggtcaa ggggcgtcgt ggcgaaggcg
     5581 ttgaaatgcc ccgtatcgaa gcagcagcag acgcgctccg atggcagggc cgcgagcagc
     5641 cgctccagtt cctgcgggtc ccgttcgtag acgttctcca gggcgatgat cgtccccatc
     5701 tgttcggcct gggggagaaa cgcgcgccac gtctccacgc tgtgctcgag ccagaggcgc
     5761 tccgccgtga cgtaatagcg gatatcgaag gaggggtggc agacaatgga gcgcggccgg
     5821 aacaggggcg cgagatcgaa tacctgctga agccgggcgg ccgtgacgcg ccggatttcg
     5881 ggatcgagcg ccccgggacg cagatcgacg aagggggcgt gcagcgtgac cggaagtccc
     5941 tcgccgacca ggcgctccgc aacggcggcg aagtcggcgc gggaataccc gtccaaggcg
     6001 acgtgggaga acccgatctc gggatggacg cgttcccgga caaccatgga gaggagcttc
     6061 tccttgagca tgggaaaggg gacatggacc tggacgtgct tcaggacaga cgacgtcatc
     6121 ttgaccttct ctctattgcc gttcgcgggt ggactttcgt ttatcatacg cccccccttt
     6181 gtacaatcgc catttttcat tgacaccccc cttcccttct gttatgaaaa tcagccagtt
     6241 aatgcaaatt taccgggagg tccgattgac cgtaaaaacc gacaaggtcc acagtgccgt
     6301 cctcacctgt ctcaacgcca tcctggagca caaagcacgg aatctcgttc ttctcaacgt
     6361 ccagaacctg tcgtccatcg ccgactactt tatcctgtgc agcggcgcct cggaccggca
     6421 ggtccaggcc atcgccgcct tcgtgcagga aacgctcaaa aagaaatccg ggctccttcc
     6481 cctcggcgtg gagggggaaa gcgtcgctca gtgggtcctg atcgattacg gggacttcat
     6541 cctccacatc ttctacgaac cgattcggga gttttacggg ctggagcgcc tctgggcgga
     6601 cgcaccccgc atggagatcg gcgacgacgt gctcaaggtc aaggccctgg cgccggagat
     6661 gtagccgtgc ggcgactcct cgcgggcctg ctcgacctga tctttccgcc ccgctgcgcc
     6721 ggctgcggac gcgtccttgg ggacgacggc ctgcccccct tctgcccggc ctgccaggcg
     6781 gaggtccgcc ttctccagtc cccgctctgc acccggtgcg gcgcgccgtt cgacggaggg
     6841 gacgcggacc acctctgcgg cgcgtgtctg gccgaccccc ccggattcac cctggcccga
     6901 tccgtcggcc ggtacgaatc ggttcttctg gacgccatcc atcgcttcaa atacggcggg
     6961 gataccgccc tcggccgcgc cctggggcgg ttcatgacgc gccacccctg cccgggaatg
     7021 gagatcgacg cggatacccg catcatcccc gttccgctcc acgtgaatcg gcttagggag
     7081 cgggggttca accaggcgtt gatcctcgcg agggaactgg cccggcccgg cggccagacg
     7141 gtggactttc tcagcctgaa gcgccgccac gcgaccgacc cccaggtccg cctgggaaag
     7201 aacgcccgcc gccggaacat ccggggggcg tttgtcgtga cccggccgga gcgcatcgcc
     7261 gaccggagaa tcctgctcgt ggacgatgtc tacaccaccg gcagcacggt cggggagtgt
     7321 gcccgcgtcc tgatggcgca ccaggccgcg gaggtcgccg tcctgacgct ggccagggca
     7381 tagcgccagc aaagacggcg gtttgaacgc gacaacgtgc ttttcgtctt gtcctcgccg
     7441 tggggaggcg cgggagtgaa aacggtcctc cggtgcgccg gagagcgaag ctttgaggac
     7501 gcctgcttta gcaaacccgt ccgttgaaga acgcaaggag tgacgcgaac catgggaaaa
     7561 atcctgtctc gtgaggccct cggggaggcc gtcgatcgtc tccaccgcgg gggaagcaaa
     7621 atcgccttca ccaacggctg ctttgatatc ctccatgtcg gccacgttca gtacctcgag
     7681 gccgcccgtc gggaagcgga cgtcctgatc gtggccgtca acagcgacgc ctcggtgcgg
     7741 gccatcaagg gcgagaaacg gccgctggtc cctgagggcg agcgggcggc ggtcgtggcg
     7801 gggttggccg cggtcgattt cgtcaccctc ttttccgaac agaccccgct cgacctcatc
     7861 cagtccctgc ggccggacgt cattgtcaaa ggcggggact ggaaagagga agacgtcgtc
     7921 ggccgggacg ccgtccgcgc ctggggcggc cgcgtcatga tcattccgga aaccgcgggc
     7981 gcctccacga cgaatctcgt ggcgaagatc ctcgaaacgt acggccggcc atgagaaacg
     8041 cctgaagcgg cgacggacca tgaactcgct tcctcctttc gattcggccg cgacggcgat
     8101 gggacaaccc tggcagcgga ccctctacat catgttcgcg gcgcagtttc tgtccatcat
     8161 cggctttgcc ttcgccctgc ccttcatccc cttctacatc cgggagctgg gtgtgaccga
     8221 cgagcgtctc gtgccggtct gggcgggcat catcaccgcc gcatcgagcc tcgtcatggc
     8281 cttcttctcc cccctctggg ggtggctcgc ggaccggtac ggccggaaga tcatggtgga
     8341 gcgggcgatg ttcgggggcg ccgtccttac cttcgccatg gggctcgtga ccgacgtctg
     8401 gcagctgctg tttctcaggc ttctgtccgg cgccacgacg ggcaccatcg cggcttccat
     8461 cgccctggtc tccaccgtgg tcccccccaa acggctgggt tacagcctgg ggattatgca
     8521 agttgccgtc tttctgggca tgaccctggg accctggatc gggggacttt tggccgacgc
     8581 cgtcggctat cgcgtcacct tcatgaccgg cggcggcatc ctcttcctgg gaggctcgct
     8641 ggtcctcctc ggcgcccgcg aacgtttcaa ccgccccgag aaagggacgc tcggaggcgg
     8701 cgaccgcctg ttctccctgc tcgcctatgc gggatttccc gccttgatgg ccctgttctt
     8761 cttcttcaac ttctccctcc acttcgtgat gcccatcctg ccgttgttca tcgaaacgat
     8821 gtgcgaccct tcccgggggg tggcctcgac gacgggcttg cttttcgcca tcagcggcgg
     8881 cgccgccgcc ctgtccgcgg gcggcatcgg cgccctcagc gaccggatgg gatacaaacc
     8941 gattctcgta acgatgctcc tgctgacctc gaccagcctc ctgctccacg gtctggcgca
     9001 gggcatcgct cacctggtcc tgctccgggt cctctacggc atcgccgcgg gggggatcct
     9061 cccgacgatg aacgccctcg tcggccgtct gatcccgccc tccggctacg gcaaggccta
     9121 cggcctcacg tcggccacga cctgcctggg aatggcggcc ggcccgtttt ttggagggtt
     9181 gatggcttcc gggtggggct accgctggcc gttcgtcttt atcagcgcga tcatggccgc
     9241 cgtgacgctc ctggtgatcc tgggacttcg gcgccgctcc caggggcgac cacccatccc
     9301 gggggaaaat aaaacttgac aggggcgcgg attcaccgta taaggcgcac gcaccctgaa
     9361 ttgatcttag tcaaaggggt gcacagttgg atatagacat tttgaagtgc aaaggcgggc
     9421 cgccgtgctc taaagagtct tggcggcctt ttctttctgt catcgtgttc atccgactta
     9481 agcgaaattt cagaaaggag gagcataaga tggcagaagg taccgtaaag tggttcaatg
     9541 agcagaaggg cttcggattc atcgagaatg ccgagggcgg cgacgtgttc gtgcattaca
     9601 gtgcgatcca ggccggtggt ttcaagacgt tggccgaggg tcagcgcgtg agtttcgacg
     9661 tcacgcaggg caaaaaaggc ccggcggcgg aaaacgtgaa acccctgtaa gggttagcgg
     9721 cactccagaa gaggcatccc ggcgcctgtt aaaggtcacg gggtgcctct ttcttttaat
     9781 gagaaggaag gaggtctttg aatggcccgg acaaaataca ccttcgaaaa aaggcagaag
     9841 gaactggcga aacgccgcaa gaaggaagag aaggcggcgc ggcgcgccga aagccggcgg
     9901 ccggcagaga acgaaacggg cgccggcacg gccggtgaag cccccgattc cgctgccgag
     9961 gcgtcgaaca cgggggccga cgccccgacg cccgaccctg cttaacgtcc cggccgcggt
    10021 cgtcgcggcc ctctccggcg ctgtccttcc ctgcgccggg gcaatttctt ctcttccgcg
    10081 gcaggcgccg cttccgtttc cgatatcccc tccccgtccc cctcggccac cagcgtctgg
    10141 ggcggggcct gcagcatccg ctgataataa tcgtccagaa gcatcgcaat caggggagat
    10201 tcgacctccg cctcggccag gcttcggccc aggtggatga agcgccgcat ccgctccctt
    10261 tcaaggctgt cgcgcgaccg caggttcgcc tccagggacg cgatgacccg ctgcgagacg
    10321 atcgcctcga catcctcgtc cgtggggagc gtcttttcct ggatgttgat gttgaagctc
    10381 cgggcgatcg accggaggtt gaactgctcc atccccgcca cgagcgtaat cgccgttccg
    10441 gtcgccccga cgcgtcccgt ccgccccgcc cgatgaatgt aaagctccgg gtcttcgggc
    10501 ggctcgtact ggaaaacatg cgaaaggtcg ggaatgtcga tgccgcgcgc ggccacgtcc
    10561 gtcgcaacga gaaagcggag cgcccctctg cgcacccggc ccatcacccg ctcccgcgcc
    10621 ccctgcgcga gatcggagct gagttcgtcg gcgtcgtagc cgaaacgctt gagcaccacg
    10681 gagacgaagt gcacggtgga cttcatgttg cagaagatga tcgcggagac gggattttcc
    10741 atctcgatga tccggacgag ggatcgctcc cgctgcatcc ccggcacgat gtagaagatg
    10801 tggctggtgc ccgtcacatg gacgtgttcc tgactcaggc tcaggaactc gggatggcgg
    10861 aggaactccc cggccaggcg gagcacgtac gacgggaagg tggcggaaaa catgtagccg
    10921 ctgatcggcc ggcgcgggag aaattgtttg acccgcttca tgtcggggta gaatcccatc
    10981 gagagcatcc ggtcggcctc atcgaaaacg atgatcttga gcgtgtccag ggagagggtc
    11041 cccttgagca gatggtcgag gatgcgcccc ggcgtcccaa cgacgaggtg cgccccccgg
    11101 cggaaggcct ccagttgggg ttcgtatttt acgcctccgt aaacgacggc cgttctaaga
    11161 ccgcgctccc cctggagggt ttcggcctcg tgcgccacct gcttggcgag ttcgcgggtg
    11221 ggaaccagga cgagtgcctg acagaccgcc ttcgccggat cgatccgctc caggatcggc
    11281 aggatgaagg cgccggtctt gccgctcccg gtttgcgact gaaccatcag gtcccgttcg
    11341 gccagcacat agggaatcgc ccgggcctgg accggcagga gcttcgtcca tcccgtccgc
    11401 gtacaggccg cctggagcgc ctcgggcagc ccctcgaggg tgatttccgg cagggcgttc
    11461 tccggctcga tcaagccatc catcccttcc cgccctgaac ctgcggcctc cgataccggc
    11521 ggcactgacg tttgggtctc tccgccgttt tccatctctt ccgacatacg tcctctcttt
    11581 cgcactttct gctgaaatat caaaattttt gcggggcgga gtataggcag aagggcgacg
    11641 ggtgtcaagg gaaatccttg aaaatcccgg caaaattcct tgacaggccg cggatgtttc
    11701 cggtactgag gggtgccgtc cctgcgccga cgaagcgccg cgaccttcgg cagacccgct
    11761 gtttccccga agacgggggc ctgcctgccc gggggcggcc ggattcccgc cacagccggc
    11821 cctcgcgttc accggtcggg agcgggagtg gcgacggaaa atgctggaag gccgagaggc
    11881 ggagcttcaa ggatcttgga cggagaaacc ggagacgtga tcgcacagga accccgcctc
    11941 gcggtgggca tcatctcccg ggcaaccggg atcagcggcg ttctgaacgg cgttttccgg
    12001 gccgatccgg agcatgtccc tgcggcggaa cggacggtgc gcctccaggg ccctttcggg
    12061 gtcttcctcg acggcgggaa accgttgctg aggaccggct ccggcgagat ccggccgctg
    12121 ggacggacgc tctccctcgt cccggaggcc gaggccgact tcaccctgaa cgacgtgacg
    12181 atcggcgtct ccttccactg ggagcgccgg gaagcacaga ccttccctgg cgggctcacg
    12241 ctgctctccc cggacgacgg cacccttacg gcgatcaacg tcgtcccgct ggagacctat
    12301 ctcgcgagcg taatttcctc cgaaatgagc gccctggccc cgccggagtt tctcaaggcc
    12361 cacgccgtca cctcccggag ctggctggcc gccatgctcg cccggcggac cgacccgggc
    12421 acggcggcac gtcccgaatc ggaaccgggc ggggcgtccc gcacctcgac ccttccggcg
    12481 gcggagggca gggagcttcc ggaaatccgc cgctggacgg gtcgggaggc gcacgcgcat
    12541 ttcgatgtct gcgccgacga ccactgtcag cgctaccagg gaatgacgaa aatcgtttcc
    12601 cccgcggcgg ccgccgccgt ctccgtcacc cggggcgtct ttctcacgca cgcgggccgg
    12661 atctgcgacg cccgctacca caaggcctgc ggcggcctca ccgagccagc ccaaaacgtc
    12721 tgggctgagg tccccatccc ctatctcacg agcgtcgccg acgcggatgg gacccttccc
    12781 cccgtgcgga cggaggcgga ggcggcggcg tggttcagtg accgccccga cgtccattgc
    12841 cgggtgacgg accccgggtt gctggcgcgg atcctgccgg acttcgaccg ggaaacggcc
    12901 gactttttcc ggtggtccgt ctcgtacgaa cgggaggagt tggaaggtct cctgaagggc
    12961 gcgtcgggga tcgacttcgg aaccctgctc gacctcatgc ccctgacgcg cggaccgtcg
    13021 ggacggatca gccgcctgcg cgtcaccggc acgaaacacg ccgtgaccgt cggccgagag
    13081 ctggagatcc gccgctggct gtcgaagagt catctgttca gcagcgcctt cgtcgtccgg
    13141 acggaacggg atcacagtgg catccctatg cggtttcacc tcgccggcgg cggctggggt
    13201 cacggggtcg gcctgtgcca gatcggcgcc gccgcgatgg ccgcgcgggg ccgcaccgcc
    13261 gaacagatcc tcgcgcatta cttccccggc gcgaccgtca cccggagata ctgacgcgca
    13321 tgggaacccc gcccgtcaaa accgccttca tcctcggcgc cggcctgggc acgcgcctca
    13381 aacccctgac cgaccgctgc cccaaaccgc tgctgcccgt cggtgggcag ccgctcatca
    13441 cccatgtcat ggaccacctg ctgtccacgg gcgtcgagcg cttcatcgtc aacacgcatc
    13501 accgggccga ggcgtacgac cgggcgtttc cggataagac ctggcggggg cgtcccatcc
    13561 tcttccgcca cgaacccgtg ctgctcgaca cgggtggcgg cctgaaaaac atcacggatc
    13621 tactggccgg agacgaccgc ctgctcgtct acaacggcga catcctgacg gacctccccc
    13681 tcgaccccct gctcgccgcg caccggcgcc gtgacgccga ggtcacgctg gccctgcggt
    13741 ccgcggggaa gcccttgaac gtgacgatcg acggcgctgg caacgtccgc gacatgcgcg
    13801 acctgctggg cgacccgggc atcaggcgct gcctgttcgc cggcatctac gtcatggaga
    13861 cccggtttct ggaacatctg cccgggggcc cgatcttttc catcgtcccg gcgctcgtgg
    13921 accggatccg ccggcggccg gggacggtgg cgggccgggt aatcgacgcg ggacgctggc
    13981 acgacatcgg ttcgcccgcc gcgtacgacg ccctgcgcaa cgcctggagc cccgcgccgt
    14041 gagcgccccg ctcccgtccg acgccgctct cctggggttc gcccgccgcg ccctgaacct
    14101 cgcggatgcg ccgcccccgg acctcgctcc catcacgcgg ggcggctcgg accgcgtcta
    14161 ccggcgcctg accgcgggcg ggtcgagctg gatcgtcatg cagtacggga cggaccgaag
    14221 agagaacgcc tccttcgccc cgatcgcgcg cttcctggcc gcccgcgaca tccgggtccc
    14281 cgtcatcctc gccgaagacg ccgcgcgggg cctgctcgtc ctggaggatc tgggcgaccg
    14341 ggacctgtgg tcgtaccgcg acgcggaccc cgccgtccgt ctctccctct accggcggac
    14401 cctgcgcctc gtccaccgcc ttcacggact ggcggccggg gacgcggccg tccgcgccct
    14461 gccgctgatg gaccccttcg acgcggcctt ctaccgctgg gagcgggatt atttccggga
    14521 acatttcgtc cgcggcgtct gcgccctcgc gctcgatccg tccgaggcgg cgggactgga
    14581 ggcggagctc gccgcgctcg ccgcgcgcct gggaggcgcc gccccctgcc tcgttcaccg
    14641 cgacctgcag tctcagaacg tcatgatccg cgacggagaa cccgtgctga tcgatttcca
    14701 ggggatgcgt ctcggatccc gcctctacga cctgggcgcc ctcctctacg acccctacgt
    14761 ccgcctgacg ccggacgtcc gcctcgacct gcttcgcgat tactacgacc tcagtccgcg
    14821 ggcgctgtcc tggaacgcct tccgggaggc cttccaggag gcgtcggtgc agcggctcct
    14881 ccaggccctg ggcgcctacg gctacctggg cctgaaggcc ggccaaccgg actttctcgc
    14941 ccatatgccc cgcggcttgg accatctgga ggcggccgca ggcctcgcgc cccgcctgga
    15001 tcatctccgc cgcctcctgt cccgctgccg ccgggcaatt aaccgttgac aggccggaag
    15061 tcatccatta tcaaggggtc atggagatcc agccgagaaa atcccgcccc ggaatcacgg
    15121 cggcgctctc gtacgccgac gcgccttggt tcgacgccct gctccggcgg ctgatccggc
    15181 agtcggcgat cgagcggatc ctcatcgcct acaacgacgg cgacgcctac ccccacgaca
    15241 agtgcgaatg gattccggcc ggctccctgg ttgcgggaga gaccctttcc accgtcatcg
    15301 agaagacgca aacgaaatac ctgctgatcc tgcccttttg cgcggacctc gaactgggtc
    15361 cccgggccct gcggcgccta ctggaggcgg cggaaatttg tcaggccggc atgctctacg
    15421 cggattacct ggaaggccat acgccctccc cggccgcgcc cgccgggaac ggggttccgc
    15481 cggcccgaaa acggggcaaa aaagacgcga acccggccct gcgggagcat cccgtcaacg
    15541 attaccagcc gggcagtctc cgtgacgatt tcgatttcgg cccgctgatg ctcttttcga
    15601 tggacgccat ccgcaccgcc ctaaagaggt acggcgcgct ttccccgttc acccacgccg
    15661 ggctgtacga cctccgcctc agggtcgcta cggacgcgac cctgtttcac ctgcgggaat
    15721 ccctggcgac ggtgatccgt ccggaaccgg ccgcaaccga cgcaccggcg gcggacgaat
    15781 gcgaagcctg gacggacgaa ggggaaacga acggcggcga cccggcgtca gcgcccttcg
    15841 cctactgcga tccccgccag caggaccgtc agcgggagat ggaaagcgcg gcgaccaccc
    15901 acctgaaacg gctcggcgcc tggctcaggc cccgcttccg gagcgtcgtg cccacctccc
    15961 aggccttccc cgtggaggcc agcgtcgtca tccccgtcag gaaccgcaag aagaccatcg
    16021 ccgacgccgt ccggagcgcc ctctcgcagg aagcggactt ccctttcaac gtcctgtgta
    16081 tcgacaacca ctcgaccgac ggaaccacgg ccgtgctgga gaagctggcc aaggcggacc
    16141 cgcgcgtgat ccccctcgtg cccgcgcgtc tcgatctcgg catcggcggc tgctggaacg
    16201 aggccctccg cgccgaagcg tgcgggcggt tcgccgtcca gctcgactcc gacgacgtgt
    16261 acagcggtcc ggacaccctg cggaagatcg tgcaatgctt ccgcgaaggc gacgtcgcca
    16321 tggtcatcgg cgcctacacg atcgtcgacg aacggggaaa catccttccg ccgggactga
    16381 tcgcccaccg cgagtggacc gacgacaacg gccgcaacaa cgccctgcgc atcaacggcc
    16441 tcggcgcccc gcgggccttt cagaccgccc tggcccgcca gatcggcttt ctgaacgtga
    16501 gttacgggga ggattacgcc atcgcgctca ggctcacccg ggactaccgg atcggccgga
    16561 tctacgaaaa cctctacctg tgccgccgct ggtccggaaa ctccgacagc gcgctgacgg
    16621 tggcgcaaat caaccgccac gacgccttca aggacaccct gcgcaccctg gaaatcatgg
    16681 cccgacggca gatgaacccg cgcccggccg tccccgtgcc ggggcgcgag ggggggcgcg
    16741 gctacccggg atacggttcg cccgcctgcc gatccggggc gccgtcatga cattctccgt
    16801 gcagccctgg cagaagcacg atctccgggc cgtccgggaa atcacctgga cgacctggca
    16861 gagtgcctac gcgcccttca tcccggccga caacctgaaa acctatttcg gcgcccacta
    16921 ttcgctgcgc gccctgcgcg ccgtgtacgc ggaccccttc gtggacggat tcatcggcac
    16981 gacggaatgc gggccggcgg ggtacctccg cacccgctac gtcggggatg agcaccggtt
    17041 ctacgtcacg tccctgtaca tcctccccga acatcagggg cgcgggctcg gcgggaagtt
    17101 gctcgcggcg gcggaggcgc gcgccctgcg ttacggggtc gacgccgtct ggctcggcgt
    17161 catggcccgg aacaaacgcg ccttggcctg gtaccggcgg cgggggttcc gtttcatccg
    17221 gaaggagccg ttcgccctgg ccgccacgac ggtcgagcac cggatcggct accggaaaat
    17281 gaacgccgcg gcggcgcggg tccctccgcc atgacgcggc cggtccggca gacccgccgc
    17341 gtccccggcg ataggatcgc cgccgttttc gaaggctcgc acggaccgcg ccccgcgggc
    17401 ggcctgtccc cactctgtcg cgcgctctac gctgaccaga cgcgatcctg gccggccctg
    17461 gcccgggccg aggcggaact ggccgggatc cgcgtccgcg atctgtcctc ggacggctgg
    17521 tcgctcaagg tccagtacaa ccccgcgcgc ctgaagagcg gcaccgcccg cgtggacccc
    17581 gcgtccatcg ccgcccgccc ctgtttcctc tgccccgcgg cactcccccc cgaacagcgg
    17641 ggcatcctgt atcgggagga gtacctgatt ctctgcaacc ccgccccgat cttccccgct
    17701 cacttcacga ttgcgagcct cgctcacgag ccccaggcca tcgaagcccg cctggaggcc
    17761 ttcctcgatc tggcccgcga tctcggcgac ggcatgaccc tcttctacaa cggccccgcc
    17821 tgcggggcct ccgccccgga ccacctccat ttccaggccg ccccggccgg cgcgatcccc
    17881 atcgaaaagg acctggcggg gcggttcgac gcccggccgg cctggggaaa caacggcgtc
    17941 gatcggtggg ggctggtctc ctgggggcga accctgatcc tgatccgggg gcgcgaacgg
    18001 acgccgctgg cccacgcaat gcgccgcctg atcgccgggg gagggacctc gccgggatcg
    18061 ccggacgaag cgatgctgaa tctcctggcc ggccaccgcc ggggggaatg gcggctctgt
    18121 ctctttcccc ggcggcgcca ccgcccggca gcctacgacc gtcccgatcc ggagcggctt
    18181 ctcgtcagcc ccggcgccgt ggacatgggc gggcttatcg tcgcgccccg ggagcgggag
    18241 ttcacccgcc tgaacgcgcc gatgctgcgg aacatctacc aagaggtttc ctcccctccc
    18301 cgggcggatc ttctcgcccg cgaatgaacg ccggcggcct gtcgcgcggg cgtccgtttt
    18361 tgcatgcaaa ggaccgatcg tcatgaccga accgtcccgg cccgtttctc ccctcgtgga
    18421 cagcgcgatg agctttccgg aggccgtcgc cggcgcgcag gcccctccgg aggcgctcgc
    18481 cccgctgagc ctcgttgacg tccgctactg ggccttcgac gggcgcctcc atgcgggaca
    18541 gctcgtcgtc caccgggacc tcgccgcgga cgtccgcgca atcttcggct ggatgcagcg
    18601 ttcccggttc cccgtggcga aggtcgtgcc gatcgtccgg tacgggtggt ccgacgacgc
    18661 ctcaatggcg gacaacaact cctccgcctt caactaccgc ttcgtcgacg ggacggagcg
    18721 gctgtcgctg cacgccctcg gccgggccgt ggacttgaac ccgcggatca atcccgtgat
    18781 ttacggggac ggacgcatcg ctccgccggg agcggcgtac cggccgggcg ctccggggac
    18841 ggtctacccc gcgcatcccc tgatggagga attccggaag cgcggctgga cgtggggggg
    18901 ggatttcaag accctgaagg actaccacca cttcgagaag cggggctgag cgcgggccgc
    18961 cccggcccgg cggggggggg gaacccttca gtcgtcgagg ggaatgaccc ggacccaagc
    19021 ggttcccccg cggaggaacc gaagttcgcg cgccgccgcg cgcgacaggt cgatgacgtc
    19081 gaagctgcgt ttgcggcagc ggtcgttaac cgtcaccacc acggatcggt cattggcgat
    19141 gttgacgacc ctgacccgtg tgccgagggg aagactggga tgggcggcgg tccgcctttc
    19201 gggacggtag atcgcgccgg agctggttcg ccggccgttg aaacgcttgg cgtaatagaa
    19261 ggcgcggcag acggagcctt cctccggttc tccgacggtg gccgactccg cgggcgccat
    19321 ccggtcggcc tccgccgccc cgctttcctg ggcgcgaagc ggcggcgcgg cggacaggag
    19381 catcagcgga ccgaccaccg ccagcagcgc aaggcctctc atcgaactgg tgcgactcat
    19441 agatcccccg gcgcggcaag acaaccccgc gcccgacgtc gttcctcttg ttcacgagcg
    19501 tccagcgcta catgaccccc ggacgtctgt caagcggttt gatggaatat tcgattgaca
    19561 tggggtccgc ttccatatag cttccttcgt ggaaacgggc gcttatagac ttcacccgga
    19621 ggagtcgccc gacacgagtg gaaacattcc gcaaaaggag gccgccatga aaacgccgca
    19681 ggaatacatc gacagcctga gaaccctcga tctcaaggtc tacctgctcg gcaaacgggt
    19741 cgagaacgtc gtggacgacc cgatcatccg tccgtcgctc aacgcggtgg cgatgacgta
    19801 cgcgctcgcc caggatcccg ggtatgcgga cctcatgacg gcgacctccc acctgacggg
    19861 caggaagatc aaccgcttca cgcacatcca ccagagcgtc gaggacctcg tgcgaaagag
    19921 caagatgggg cggctcatgg ggtcccgcac cggctgctgc ttccagcgtt gcgtcggcat
    19981 ggacgcgctc aacgccctgt cgatgacgac ctacgacatc gatcaaaggc acgggacgga
    20041 atacaacaag cggttcctga aatatctgga atacgtccag gagaacgacc tcgtgtgcga
    20101 cggggcgatg acggacccca agggcgaccg ctccctgccg ccccaccggc aggccgatcc
    20161 ggaccagtac ctccggatcg tatcggaaag ccgggagggg atcgtcgtgc gcggggccaa
    20221 ggcccaccag accggcgccg tcaactccca cgaggtgatc gtcatgccca cgatcgccct
    20281 gcgggaggag gatcgggact acgcggtctc cttcgccctg cccagcgaca cggcgggcat
    20341 cacctacatc cttggccgcc agtcctgcga cacccggaag ctggagggcg gccggatgga
    20401 cgtgggaaac gtcatcttcg gcggacacga ggccctgatc gtcttcgacg acgtcttcgt
    20461 cccgtgggaa cgggtcttca tgtgccgaga atacggcttc gccggccagc tggtggagca
    20521 gttcgcctcc taccaccgcc agagctacgc atgcaaggtg ggggtcgggg acgtcctgat
    20581 cggcgcggcg cagacggccg ccgagtacaa cggcgccgag aaggcctccc acgtgaagga
    20641 caagatcatc gagatgaacc acctgaacga gaccctctac tgcggctgca tcgcctgcgc
    20701 cgcggagggg caccgcgagc cgtccggaac ctacctggtc gacacgctcc tggccaacgt
    20761 gcacaagcag aacgtcaccc gcttccccta cgagatcgcc cgcctcgccc aggacatcgc
    20821 cggcggcctg atggtcaccc tgccctccga ggccgatctc aacgcaccgg agacgggcaa
    20881 atggatgcaa aaatacttcc gcggccgcag cgacatcccg acggaagaac ggatgcgcat
    20941 cctgcgcctg atcgaaaacc tcacgctggg gacggcggcg gtcggctacc tgacggaatc
    21001 catgcacggg gcgggctcgc cgcaggcgca gcggatcatg atcgcccgcc tggtcgacct
    21061 caagcaaaag caggcgttcg ccaaggccct gtgcgggatc cgggacgcga agccgcggtg
    21121 agccacccgc gcatgcgtgt cattcaacgg ccctgattcg cggacggacg gacgggaacg
    21181 ccccaatccg gcggcacgga aaggagacag gcaaatggac cgaaacgtgg gaaccatcga
    21241 caggatcgtc cggctcgtga tcgcgatctt tttcatcgcc gccaacgccg cgggctgtgt
    21301 gacgggcctc gcggggctcg tcctcgccgt cctggcgggc atgctcctct cgagcgtggc
    21361 ctcggggcac tgcccgctct acgtcaggct gggcatcaag aaatcggtct gagcgccgaa
    21421 cggaccgcgg ccattcccgg cttgacggcg caacgccttt gccatacaag ggtccgcggg
    21481 gcgagccgga tttccatgtc ccggccgggg ggttccgcgc ccggtcgtcg aaaacggcga
    21541 ggcggatcag caaaagcggg gagacggcgc gcatgccggg tctgaagctg taccacagca
    21601 accggatgga ggtcctggcg gaccggctgg cggaggtcct ggcggttccc ctcgcggatc
    21661 cgttcgcccc ggagatcgtc ctcgtccaga gccgcggcat ggagcgctgg ctgtcgatga
    21721 ggctcgcgga ccgtcacggc gtctgcgcaa acgtccgctt cccctacccc acccatttcg
    21781 tccacgccct cttccgggcg gtcgatcccg ggctgccggc gcgctcgccg tacgaacccg
    21841 cggtcatgac ctgggatctc gtcgacctcc tgtccgccct tcccgaccgc ccggagtacc
    21901 ggccgctcaa ggcctatctg gacgacgacg gcaacggcct caagcggttc cagctcgccg
    21961 cccgggtcgc ggcgctcttc gaccagtacc tcctgttccg tcccgattgg atgccggcgt
    22021 gggagcgcgg gcaggagacg cactggcagg cggcgctctg gcgggagatc gtccgccgca
    22081 aggggccggt gcaccgggtg gcgctccggc gaaaactcct ggcggcgatc gcggacgcga
    22141 ccgttccgct ccccggggcc cccgaacggg tcgccatgtt cggcatctcc tccctgcccc
    22201 ccttctacat gcagattctc tcggccctcg cggcccggat cgaggtgaac ctcttcctcg
    22261 tcaatccgtg ccgggagttt tgggaagaca tcgtctccga gcgcgaggcg ggccggatcg
    22321 cggagcgtct ggctgggtac gaccgccgcc ccgaggcgta tcacctcgag gcggggaacc
    22381 ggctgctggc gtccttgggt cccctcgtgc gggagttcct gcggacaatc gccgaactgt
    22441 ccgccgagga attcccgcaa ttcgtagatc cgggcgacgc caccctgctg gcggcgatcc
    22501 agtccgacat cctgaacttg acggagcggg tgccggcggg cggcgacgct cccgccgcgg
    22561 acgacgggtc actccgggtg cacgtctgcc acagtccgct ccgcgaggcg gagatcctgc
    22621 acgacgctct gctcgcgatg ttcgacgcgg atcgaacgct ccggccggat gagatcctcg
    22681 tcatgatccc ggacatcgcg gcgtatgcgc cgttcatcca agccgtcttt tcggcggcgg
    22741 cggacgatcc ccgcgccatc cccttcacgc ttgccgatcg gggatacctg cacgagagcc
    22801 atttgatcga cacattcctc cacctcctcg acctcgcggg gagccgctat ggcgccgcta
    22861 aggtgctcga ccttctggac gcgcccgccg tccgggaaaa attttcgctc gcggacgacg
    22921 acgtgcgtct cgtccggcac tgggtccggg aaacgggcat ccgctggggt atcgacgccc
    22981 ggagccggga ggcgctggac ctaccggcgg aggccgccaa cacctggcgg gccggcctgg
    23041 accggatgct gctcggctac gccctgccgg cgggcggcga ggccgtcctc gtggacggca
    23101 tcttccccta cgacgacatc gaggggggcg ccgcggagat tctgggcccc ttcctcgcct
    23161 tcacggaagc gctcttcacg gccatccccg ccttggagac cgggcgaacc ctctccggat
    23221 gggcgcaggc gctcaccgcg cttttggaga cgttcttttc cgacgacgag cgctacggtg
    23281 cggaaacgca ggccctccgc cgtcttctcg gggagctgcc ggaactggag gcggcctccg
    23341 ggtatgcggg ggaaatcggg atcgaggcgg tcaaggcctt tctccgcgag aacctcacgg
    23401 acagggggtc cggcggcggc tttctcgcgg gcggcgtcac attctgcacc ctgctcccca
    23461 tgcgcagcat cccgttcaag gtcatcgcgc ttttgggcat gaacgacgac gcctatcccc
    23521 ggccgtcggt cccgctcgga ttcgaccgca tggcgtccgc gccgcgcccc ggcgaccgtt
    23581 cgcgccgcca ggacgaccgc tacctcttcc tcgaggcgct cctgtcggcc cggcagcgcc
    23641 tccatatcag ctattgcggg cagagcccga aggacaaccg cccccgcccc ccctcggtcc
    23701 tggtgagcga actgctcgat gccatcgagg cgggcttcgg cccgcgcgta cgggaagacc
    23761 tttgcacgca ccaccgcctg caggccttcg acccggccta tttcagcggc gatccgaagc
    23821 tgtacagcta ctccacggag aacctccgag gcgcggcacg cgccgccgca ccgcgccggg
    23881 aacccggggc cttcatcccc tccggccttc ccgagccggg agcgggcctg acggagatcg
    23941 acgtcgaaac gttctgcgcc ttcttcgcac acccggcccg cttcctcctc cgccgccggc
    24001 tcggccttta tctgcgcgac cgggaggcgg cgatcccgga ggaggagccc ttcaccctcg
    24061 agggcctaga cgcgtacggc ctcggtcagg acctggtcgc gcgtctgctg gcggaccgcg
    24121 atatcgcgca ggcctatccc gtcctcaagg ccgccgggcg tctcccccac ggcgcgccgg
    24181 gggcgtacgc ttacgaccgt ctgcacgatg ccatgaccgg gctcgccgcc gacatcggcc
    24241 gtgccgtctc cggcgggcgg atcgaggccg ttccggtgga cctccgcctg gcgggggtgc
    24301 atctcaccgg cgcgctggac ccgctctatc cggagggcct ggtccgctac cggccggccg
    24361 acgtgaaggc ccgagaccgc ctggacgcct ggatccggca cctgatcctg aaccatcccg
    24421 gttgcgcggc acccgtccgc gagacgcgct tcatcggccg ggaccgcggg gtccgctacg
    24481 cgcccctcgc cggcgcggag caacccctgg cggacctggt caccctcttc ctcgcgggac
    24541 tccgcacccc cctgcacttc ttccccgaat cctcgctcgc ctacgccgac gcaaccgcgg
    24601 cgggcaagga tgcggcggcg gcgctccgtg cggcgcgggc caaatgggag gcctttcgct
    24661 atccggaaaa ggacgatccc gacaacgagc tctgcttcga ccggatcgac cccctggacg
    24721 acgccttccg ccgcctggcc gagacggtct tccatccgct gctcgcggcc gaggaggcgc
    24781 tgacctagcc atggacgccc cggagatctt caaccttctc gagagcccac tgacggaaac
    24841 caaccttatc gaggccagcg ccggcacggg aaagacctac gccgtggagg gcatcttcct
    24901 ccgcctcctt ctggaacggg ggctttccgt gaaggagatc ctcgtcgtca cctacacggt
    24961 cgcggcgacg gaggagctcc gggaccgcat ccgcggacgg gttcgcgccg ccgtcgccgc
    25021 gttcacgtcg ggcggccacg acgatccgtt tctcgacggc ctggtccggc gcacgacggc
    25081 ccgcgaagag gcgctccgcc gccttcgggc ggccctccgg gatttcgacg aggcgccgat
    25141 ccacacgatc catggcttct gtcagcgggc gctccgcgag cacgcctttg aaacgcgggg
    25201 gacgttcgac gcggagatga ttccggacga ccgcgccctc aaggaggaga tcgtccggga
    25261 cttctggcgg cggcacctct acgggggggc gccggaggtc ccggggcacg ccctggcgag
    25321 gaagttcggc ccggaaggct ttctcgaact gatcgaggga cgcctcttcg atcccggcat
    25381 gcgcatcctc cccgaggccc cgcccgtcgt tctggacgcc ctgcccccct tccgggaggc
    25441 ggtggaagcc gtgcgccggg cttggccggc ggcccgggat gccgtcgcgg cgtgcctgcg
    25501 cgaccccggt ctgaagcgga acatttaccg gaatccggac gcccttctgg cggagatgga
    25561 cgcctacacg gccgcccgca ggacgtggcc cccgttcgac ggtttcgaca ggttcaccgc
    25621 gaccgccatc gagcagggaa cccgcaaggg ctgcacgccc ccggagcacc cctttttcgc
    25681 cgcctgcgaa acgctccggg agcgggcggc ggcgtggacg gagcaggccg accggcacct
    25741 cctgttcctc aaggccgaac tcttccgcac cgtgcgggcg gaactcaggc aacgaaaacg
    25801 gcggcggaac atccagtgtt tcgacgacct tctcctcgat ctgcgggagg ccctggcggg
    25861 gccgggcggg tcggcgctcg cggaaaaact gcgcgcccgc taccgggccg ccgtgatcga
    25921 tgaatttcag gacaccgacc cgatccaata cgccatcttc gagacggtct tcggccacgg
    25981 ggacgccacc cttttcctga tcggcgaccc caagcagtcc atctacagct tccggggggc
    26041 cgacctgttc acctatctgc gggcctccca cgacgtgcgc cgccgccaca ccctgcggca
    26101 caactggcgg tccgaaccgg ccctgatcga ggccgtcaac gcggtcttcc gtcatccgga
    26161 cggcgcgccc ttcctctacg acgcaatccc cttcgccgag gccatcgcgg gggacgtccc
    26221 ggaacgggcc gtcctgacgg tggacggaag gccctccccc ccgttccggc tctggtttct
    26281 cgacggggtg cgcctcgaag cggcgggggc ggcgaagaac caggaccgcg cggccgatct
    26341 gatcatccgg tccgtggcgg cggagatctc tcgcctcctc gacccgagga aggggccccg
    26401 gacgacgatc ggcgatgccc ccttggtcgc cggcgacatc gcggtcctgg tccgcaccaa
    26461 ccgggaagcg cgtcgggtgc gggaggccct ccgtaccctg ggcatcccgg cggtcctcca
    26521 cagtacgggc aacctcttcg acacccgtga ggccatcgag atcgagcgcg tcctgggggc
    26581 gctggccgcc ccccgggacg agggccggat acggatcgcc ctcgcgaccg acctgatggg
    26641 tctgaacgga gaggcgctcc acgcgctcag cggggacgag accgcctggg aggaccggct
    26701 cgcccggttc cgggcctacc acgccctctg ggacgagcgg ggcttcatcc ccatgttccg
    26761 acgcttcctg gccgcggaga acgtccgcga gcggctcctc gcctttcccg acggtgagcg
    26821 gcggctgacg aacgtccttc acctcgcgga ggttttgcat gcggaagccg tctccggacg
    26881 gcagggcatg gcgggcctcg tccagtggct cgcccggcag cgggatcccg tgacgccccg
    26941 cctggaggag cacgaactcc gcctggaaag cgacgcgcgc gccgtgaaca tcgtcaccat
    27001 tcataagagc aagggtctgg aataccccgt tgtcttctgc cccttccact ggaggggcac
    27061 caaactccgg cagggggaat tcctctttca cgaccccgcg gacgactggc ggcccaccct
    27121 cgtcctcacg tcggacgccg gcaccggccg ggacctggcg ctccgggaaa cgttggcgga
    27181 agacatccgc ctgctctacg tcgccctgac ccgcgcccgg caccgctgct acctcgtctg
    27241 ggggccgttc aaggacgcgg gcgcctcggc cctcgcgtat atccttcacc cgacggccgg
    27301 ggaaaccggc gacggcacgg ccgcgacgga ggccggagcc acgggactcg acggcaacgc
    27361 cctgcgccgg gagttggagg ccctcgccgc atccgtcccg ggggcgatcg ccctggagga
    27421 gatgccgccg ccggacgccg ctccccgcgc cgcttccccc gaagcggaga cagccctgac
    27481 ctgccggacc ttcaccggcc tgccggacag ggaccggccg atcgccagtt tttcttcact
    27541 cgtggcggac cgcgccctcc ggccggaatc cccggccgaa atcggcgacc ccgccgacct
    27601 tcccgaccgg gacggcgagg gcggggcggc cgcgccgccg ccggccggga aaccgtccgg
    27661 ggtgttcgcg ctcccgcggg ggacgaaaac gggcaacctc ctgcacgaga ttctcgagga
    27721 gatcgacttc accgccgcgt cgggcccagc ggcggaaaga ctgttcgcgg acaagctcgc
    27781 ggcgtacggg ttcgacccgg cctgccggga gacggtcgcc cggacaatcc ggctcgccct
    27841 cgacacgccg ctggacccgg atctcgatgg actgacgctc tccaccgtcg gccggacgga
    27901 tcgcctgagc gaactcgggt tctacgtccc cctgaaccgg ctgacgccgg accgtctgga
    27961 gcgcctgctc gccgaaaccg ggatcgcgcc gacgtcgggt tttccggaag ggatcggccg
    28021 gctccgcttc cagccggtcc gcggcttcat gcgggggtac atggatctcg tcttccgacg
    28081 cgccgggcgc gtctatctcg tggattggaa aacgaacttt ctgggcgaca ccgtggcgga
    28141 ttaccggccc ggggccctgg cccaggcgat gcgggaggcc ttttatccgc tccagtacca
    28201 cctgtacgtg ctcgccctgc accgctacct caagcggcgt ctgccgggat acgactacga
    28261 cacccatttc ggcggcgttt tctatctctt cctgcggggc ctcgattcgg gaaattcggg
    28321 cgctacgggc atctaccgcg atcgcccggc ccgggaaacg atcgcggccc tcgaacgggc
    28381 cctgctcccc gccgcggccg gaagcgacgg aagggaggag gtcccatgaa aaacgatgca
    28441 ggcagcctcc cgccccggtc gcgcttctca ccgcttgacg aggccttcgg ccggttcatc
    28501 cgccgaatcg acgggcggga cgcgccggag ctggccctcg ccgcggcctg gctcagccgc
    28561 tcccgggccg agggccacat ctgcctcgat ctgggggcac gaccggaaga cggttttccg
    28621 gtcgccgggg agggcctccc ggcccgtccc gaggccgaac gctggaacgc cctcctggaa
    28681 acgagccccg cggtgggaag gcccggggac cggctgccgc tcatcctgga cgggacccgt
    28741 ctttacctgt tccgctactg ggcctacgag caagcggtgg tccgcacgct cgacaccctg
    28801 gccgtcgatg ccgcgggcgt cgccgacgag gcacggctgc gggcggcttt tgactgcatt
    28861 ttccccccga acggcggagc ggagcggatc gactggcaaa aagtcgccgc cttcgccgcg
    28921 gcgagacacc ggttctgcgt cgtctcgggc gggccgggca cgggcaagac ctccaccgtg
    28981 gcccgcatcc tggccctcct cgtcgcccag gaccccgacc gcccccccgc cgtcgccctg
    29041 gcggccccga ccggaaaggc ggcggcgcga ctgcaggaat ccatccggga agccaaggcg
    29101 acactcgcgc ttccccccgc gctcggggcg gcgatccccg aaaccgcctc gaccctccac
    29161 cggctgctcc agagccgccc ggagacgccc tatttccgtt accatgcgga tcgccccctg
    29221 ccggccgacg tcgtcatcgt cgacgaggcg tcgatgttgg acctcgccct cttcgccaaa
    29281 ctgatcgggg cactccgcac ccgcgcacgt ctcatcctcc tgggagacaa ggaccagctg
    29341 gcctccgtcg aggccggggc cgtcctgggc gacatctgcg ataccgggcg ggctcacggc
    29401 tactcccccg ccttcgccga cgcctgcctg cgggtgacgg gggagcgcat cccggcagac
    29461 gacgtgtcgg cgcccggccg cttgaacgat cgcatcgttt cgctccggaa gagctaccgc
    29521 ttcggcgaaa cgagcggcat cggcgccctg agccgggccg tcaatgcggg tgacgcgtcc
    29581 cgggccgggg cgctcatccg ggacgcggga accggcgacg tcgcctggcg ggagctgccg
    29641 ccccccgagg ccctgccgcg gctgctggcg gcctggatcg gaacgcacga catcgcgccc
    29701 ccgacggcgg gcgacccggc cgaactcctg gagcgcttcg gccgcgcacg gatgctctgc
    29761 gccctgcggg aggggcctta cggcgtccgg cacatgaacc tcctcgcgga gcgggttctg
    29821 cgggagacgg gacggatcgg cggaggcagc ctctggtacg cggggcggcc cgtgatggtg
    29881 acgaagaacg actacggcct ggggctgttc aacggagaca tcgggatcgc gctggccgat
    29941 ccggaacacc ggggcgatct gcgggtcttc ttcccagccg gcggcggaag ggtgcgaagc
    30001 ttcccgccgc tcaggcttcc ggagcacgaa tcggtctacg ccatgacggt tcacaagagc
    30061 cagggttccg aattcgacga tgtcctgttc ctgatgccgg accgagacgt cccggtgctg
    30121 acgcggcagc tcgtctatac ggccgtcaca cgggcccgga gaaccctgtg gatcgccggt
    30181 cgggaggagc ttttcgcggc ggccctgagc cgccacctga agcgcgcctc cggactccgg
    30241 gacgctctct ggggcagcgg aacgacctgc gcctgacccg gaccggtccg gtcggcggcg
    30301 gcggccctaa gccacccgtc tcagttttcc attgacacga agtcgacttc ccgctatcaa
    30361 cacgccgtaa acgcttttcc cgatgagcgc gacccgcaaa ggacagcacg gtgacgcact
    30421 ttcctggcca acatcttccg gaacatcagc gcggcctcct gcatcggttt tacgccatca
    30481 gctttctgac aattctcatc cccgtcctcg tcgtctctta cctgatctac tcgacgggtc
    30541 tttccctcga cgacaatcac ctgctcatgt ttgccatgac gctgctgctc atgctggcgg
    30601 ggctggtcat cctccgttac gtcttcaccg gcatcatggc cgtggtggac atcctgaaac
    30661 agcgcgaccc ggaggacgga tcccttcccc tggtcatccg ggaggacgtt tcggagctga
    30721 aagaaatttc cgcgtgcctg gcgaacctca tgaaccggtt cgagaagacg acgggcgagc
    30781 tggaccggcg ggtcttcgaa ctcaacaccg tcaacgagat gtcggagatc acccgcaaga
    30841 cgctgcggat ggacgagctg ctcaaggtcg tgctggaaaa ggccatggcg gtggtcgggg
    30901 cgcaaaccgg atcgatcttc accgtggacg gcgacgcgaa accgccgcgg ctggtggcct
    30961 cctcgccgcc gggggccgtt ccgaacggga cctccgtcaa tctcgagcgg tccttcatga
    31021 agcgcgtcat cgaggaccgg aaaa
//
LOCUS       DAOOLJ010000019        28966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_1417_length_28966_cov_16.215869, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000019 DAOOLJ010000000
VERSION     DAOOLJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 28966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28966
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_28966_cov_16.215869"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLW16_05210"
     CDS             complement(<1..845)
                     /locus_tag="PLW16_05210"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPB70339.1"
                     /translation="MANVVVVGAQWGDEGKGKVVDLYAEDADVIVRFQGGNNAGHTLV
                     VNGQQTILHLIPSGILHDHKFCIIGNGVVVDPKVLIEEIDSLNGRNLFPAHTKLYVSE
                     NAHIIMPYHRRLDAAREAHKAGTKIGTTGRGIGPAYEDKAARVGIRLSDLLDEGTFRE
                     KLRANCAEKNFMLTRYFGEEAVDEAAIFEEYRAYADRLRPYAADASLILYKEIKKGKK
                     ILFEGAQGNHLDIDHGTYPYVTSSNTVAGNAGAGAGVGPGALQSVVGICKAYTTRVGE
                     GPFVT"
     gene            complement(861..2447)
                     /gene="serA"
                     /locus_tag="PLW16_05215"
     CDS             complement(861..2447)
                     /gene="serA"
                     /locus_tag="PLW16_05215"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPB70340.1"
                     /translation="MTKRVLVSDTLSPEGVEIFRQAPGIEVDVITNLTPDELKGVIKD
                     YDGLAIRSATKVTKEIIAAADNLKVVGRAGIGLDNVDIAAASKRGIVVMNTPGGNTIT
                     TGEHAIAMMMALARQIPQATASMKDSKWEKNRFMGSEVCNKTLGIIGIGRVGTIVADR
                     AQGLKMTVIAYDPFISAEAAENLGIALVTLDELLAKSDFISVHTPMTKETRGIINAAA
                     FKKMKKGVYIINCARGGIITEKDLYDALVSGQVAGAALDVFEEEPTKNKDLVGLPNVI
                     CTPHLGASTDEAQINVAIAVAEQIVGYLTRGEIRNAVNFPSVSAELLNVIQPYLGLAE
                     KLGKFEAQLITGGIEQVKIEYSGEIINHNVAPITISLLKGLLTPILNENVNYINAPVV
                     AKERGIRVVEAKSSEVKDYTSMITLSVKTAQGESCAAGAIFGRQDARIVRIDKFTVEV
                     NPDGLMLVLYNIDRPGVIGNIGSTLGAHDINIARMHLSREQVDGQALVVLSTDSVVPT
                     EVMEKLRSLPHVISVQALEM"
     gene            complement(2465..3589)
                     /locus_tag="PLW16_05220"
     CDS             complement(2465..3589)
                     /locus_tag="PLW16_05220"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HPB70341.1"
                     /translation="MNPNFSSGPCGKRPRWTLGALEDAAVGRSHRSALGKEKLTRAIT
                     ETKRILGIPADYLVGIMPASDTGAFEAAMWSLLGPRPVTVLVWESFSDGWATDVARQL
                     KLKPTILKADYGKLPDLNAVDWRQDVVFVANGTTSGVKVPHWDWIPDDREGLALCDAT
                     SAVFAMPIAWEKIDALTFSWQKCLGGEAAHGMLVLSPRAVARIEAYDPPWPMPKIFRM
                     KKGGRINAGIFEGDTINTPSMLCVEDYLDALKWAEAEGGLDALIARSMGNLRIVSEWV
                     QKTDWVEFLAADPTVRSNTSVCLMVTSARVAALPKDERAKFLKGIASELARQKIAYDI
                     NSYKDAPPGFRFWCGPTVAAEDLRAALAALEKAYLQKADA"
     gene            3735..5093
                     /gene="radA"
                     /locus_tag="PLW16_05225"
     CDS             3735..5093
                     /gene="radA"
                     /locus_tag="PLW16_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPB70342.1"
                     /translation="MKKIKTSFFCQSCGHASTKWLGKCPSCGGWNQFVEEEIREASEG
                     PSSDLQLHEAPRPIADIVADEKERLATGLAEMDRVLGGGVVPGSAVLVGGDPGIGKST
                     LLLQVCQRLAERGLKVLYCSGEESARQIKLRGKRIGADSDQLLVFVEIALENILAEIR
                     RIEPHAVVIDSIQTVYASNLSSAPGSVGQVREASERLILHAKKTGIPVFLVGHVTKDG
                     SLAGPKVLEHMVDTVLYFEGDSGHAYRIVRGIKNRFGPTNEIGVFEMRDDGLKEVANP
                     SALFLSERPEKAAGSVVVPSLEGTRPILVEIQSLVIPTSFGMPRRTTIGVDHNRVSLL
                     VAVMDKVCGLHLSGHDIFINVAGGVKVDEPAVDLGLVASMASSFLDRPVAPGTVVFGE
                     VGLTGEVRGISQMETRLKEAARMGFTRCILPAGTPAGRRPESGIEMFGIRSLKDLLDP
                     LF"
     gene            5266..5868
                     /locus_tag="PLW16_05230"
     CDS             5266..5868
                     /locus_tag="PLW16_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70343.1"
                     /translation="MVEKIFWLGHSSVRIQSGTVLYIDPFQIADGPEADLILISHAHH
                     DHFSPEDIRKIRGKETVIVTTPDVAAQLTGEVRPVRPGDRLTVGPVAIEAVSAYNLDK
                     PFHPKKNDWVGFILTVDGKRIYYAGDTDDVPEMQALKADIAILPVGGTYTMTAAEAAA
                     VANRLRPEAAIPVHYGTIVGSADDARRFAELCRVPVFIEG"
     gene            6248..6538
                     /gene="groES"
                     /locus_tag="PLW16_05235"
     CDS             6248..6538
                     /gene="groES"
                     /locus_tag="PLW16_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB70344.1"
                     /translation="MKIRPLQDRVIVKRLEEEQKTKGGIIIPDTAKEKPMEGKVIAVG
                     KGKTTEDGKLVKPDVKAGDRILFSKYAGTEVKIDGEEHLIMREDDILGVIEK"
     gene            6564..8201
                     /gene="groL"
                     /locus_tag="PLW16_05240"
     CDS             6564..8201
                     /gene="groL"
                     /locus_tag="PLW16_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPB70345.1"
                     /translation="MGAKLIQYDEEARKSILKGVNTLADAVKVTLGPKGRNVILDKSF
                     GAPTVTKDGVTVAKEIELEDKFENMGAQMVREVASRTSDVAGDGTTTATILAQAIYRE
                     GAKTVAAGSNPMDLKRGIDKAVEVVVNELKKQSKPTKDQKEIAQVGTISANSDETIGD
                     IIAEAMGKVGKEGVITVEEAKGLETELEIVEGMQFDRGYISPYFVTNAEKMVVDLEDC
                     YILINEKKISGMKDLLPILEQIAKMSRPLLIIAEDIEGEALATLVVNKLRGTLHVAAV
                     KAPGFGDRRKAMLEDIAILTGGKMISEDMGAKLENAQLADLGRAKRITIDKDNTTIID
                     GAGDRKALEGRVKQIRAQVEETTSDYDKEKLQERLAKLVGGVAVIKVGAATETEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVAYLRTLPALEKLKLEGDEAVGVSIVRKSLEEPM
                     KMIAANAGLEGSIVVEKVKEKKGAFGFNARSDKYEDMIAAGVIDPTKVTRFALQNAAS
                     VAGLMLTTQCMIADKPEDKSAAMPPMPPGGGYPGMGM"
     gene            8331..8738
                     /locus_tag="PLW16_05245"
     CDS             8331..8738
                     /locus_tag="PLW16_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPB70346.1"
                     /translation="MKGILIRWFILTLAILLSAYVIDGIIVTGFFPALFAAAVLGILN
                     AFFRPVLLILTLPVNIVTLGLFTFVINALLLKMASGVISGFDIHGFWAAVLGALVISV
                     VSWFLNSFVSGQGRIGPNEVIDLKRRGDGGRWE"
     gene            8735..11365
                     /gene="mutS"
                     /locus_tag="PLW16_05250"
     CDS             8735..11365
                     /gene="mutS"
                     /locus_tag="PLW16_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPB70347.1"
                     /translation="MTGTPAALPPAMQQYVQTKARYEDCILLFRMGDFYEMFFDDAVT
                     ASRVLDLTLTSRNKGKEDSIPLCGFPYHAASTYIAKLIAQGFKVAICEQMEDPKLAKG
                     VVKREVVRVVTPGLVVEADNLEAKENNFLAALAFADGAYGLAFLDISTGEFRLAEFRD
                     RDAFLDEVASLSFREILLEETFPERERLGGGPESETRRRIETVAADYFAPAPARELLR
                     DRFSIEALRDLNVESFPAALAAAGAILRYAVETQQDRLGHVNALQWYESSQNLILDDI
                     ARRNLELFSTITEGRKAGSLYHVLDETMTAMGGRRLRWWLSYPLCQPERIRKRLAAVA
                     DLKEHHLLRQNLRRALGRQHDLERLGGRIAMGVANARDLVALKQTLQALPEVKGLLAD
                     LSAPLLVAIREGIDEMSDVLALAESAIADDPPLSLRDGGLIREGFDAELDRLTSIGRD
                     GRKWIAALEERERRRTGIGSLKVAYNSVFGYYIEITKANADHVPDDYIRKQTLVNAER
                     YINQELKEYEYTVLNADDARRQREYDLFVDVRERMAGQIRRMQKTAGLLADLDALAAL
                     AEVAERYGYTCPVVDDEDVIEIRDGRHPVVERMPLPEGFVPNDVLLDLDRNRLLVITG
                     PNMAGKSTYIRQVALIVLMAQMGSFVPAASARIGCADRIFTRVGAADSLARGQSTFMV
                     EMTEVANILSRATSRSLVILDEVGRGTSTFDGLSIAWAVAERLHDAAPRGVRSLFATH
                     YHQLTELATTKAGVANYNIAVKEWGSRIIFLRKIKEGGTNRSYGIQVARIAGVPEEVI
                     SRAKEILDNLEKGELDPTGKPKLARGRRPAERNPDQLGLFMGEEERVLQELRDADIPN
                     LTPVEALNRLNEWKKRLGKG"
     gene            complement(11405..13699)
                     /locus_tag="PLW16_05255"
     CDS             complement(11405..13699)
                     /locus_tag="PLW16_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPB70348.1"
                     /translation="MDASPKNQQISALEEKLQIRKALQDITNRIHAAQNTKQILVDLK
                     DGILNLFDAHSITIYVVDRPKNEIYSMFLVGSSVREIRVPINNRSIAGFVANNGRVVN
                     IADAYDVEALKQIDKDLSFDLSWDKKSGFRTKQILAAPIFHNQTLMGVVQILNKKGGG
                     PFSVEETDFLQEIAAVLGVAFYNQERFARRRKTRFDYLISRDLLKEEELENAWEESRE
                     QKETMETFLMRKHKISKEDLGKSFEEFFRCKFIQFSDKIPIPGDLLKNLKRDYLRREL
                     WVPISKVDGNIQVIVDDPNNILKRDMIENLLRTKAVKYDVALAEDIVKFINYFFQTPE
                     DESSISDLLGKLEADEAPDEEDDSGDVVTESDSVIMQLVNKIINDAYIRRCSDIHIEP
                     NVGRKNVEIRYRVDGDCALYQTLPYSYRSAIISRIKIMSNLDITVKRLPQDGKIKFKR
                     TGGDEIELRVATIPTQGGVEDVVMRILAKGETLPLEAMGMLPRNYGELLKILEKPYGM
                     VLVVGPTGSGKTTTLHAALHHINTPDRKIWTAEDPVEITQYGLRQVQVQPKIGFDFAA
                     AMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETITRLLDMGI
                     DPLNFADALLGILAQRLVRTLCKKCKEAYHPTPQEYEEMVESYGPEAFAKLNVPYTDD
                     LTLYRPKGCDACDKTGYKGRMGIHELLAGTDDIKKLIQKHATIEEMRDVAMTEGMTTL
                     LQDGIQKSIQGLTDFKQVRRVCIK"
     gene            complement(13885..15957)
                     /gene="glyS"
                     /locus_tag="PLW16_05260"
     CDS             complement(13885..15957)
                     /gene="glyS"
                     /locus_tag="PLW16_05260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPB70349.1"
                     /translation="MGKELLLEIGTEEIPAAFLPKAMRDLEEMSRREFSACRLAHGAI
                     QSLATPRRLCLVVANLAGGQEDLEVEKMGPAKKAAFDAQGQPTKAALGFARGQGVELG
                     DVGTAVTEKGEYLCVRKTIAGEPTEALLPEILPRLILGLPFKKSMRWSDLDIRFARPI
                     HWLLALYGGRVVPFTLGNIQSGNLTRGHRFMSPEAVSVKDFADYLARTKERCVIVDPA
                     ERKAIIRQETRKAAAQVSGRALEDETLLEQVVFLTEYPTVVCGSFDRDYLRLPQEVLV
                     TSMISHQKYFPVVDAEGRLLPHFLAVNNTLARDPSVVARGNEKVIRARLSDAHFFFTE
                     DQKVPLTERVEGLKQVVYHTQLGTSYEKVMRFRALALTLADRIDPTLKATVERTALLA
                     KADLDTQMVGEFSELQGVMGREYALLAGENPTVARAIYEHYLPTQAGGALPATDEGAI
                     VSIADKMDTIAGFFAVKLIPTGTADPYALRRQALGIINILLAKRYPIRLDELVDLGLS
                     LLEEKAKRPAGETRADILDFFRGRLENQLIAQGRPYDVVDAVLATGISDLVRTVKKIE
                     AMEAFKDHENFEPLAIAFKRVGNILKDFPGGSVREDLLETAEERTLYQSFREIRTRAL
                     THLDAEDYPSALVEMAGIRQSVDAFFETVLVMAKEEAIRNNRLALLAEISALFHRVAD
                     FAKIVTSV"
     gene            complement(15986..16864)
                     /locus_tag="PLW16_05265"
     CDS             complement(15986..16864)
                     /locus_tag="PLW16_05265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPB70350.1"
                     /translation="MALTFQELIFALERYWASQGCVIQQPYDLEVGAGTFNPATFLRT
                     LGPEPWNVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPVDIQERYLNSLKSFG
                     IDPLEHDIRFVEDDWESPTVGAWGLGWEVWLDGMEISQFTYFQQVGGVDLKPISVELT
                     YGIERIAMYIQGIDNVYDLKWNDTVKYGDVHHQGEVEWSTYNFQYADAEMLRKRFDMD
                     EAEGIRMAEKDLVLPTYDHCLKCSHTFNLLNARGAISVAERTSYIGRVRNLARLSAEG
                     WLRQRERLGYPLLDRG"
     gene            complement(16967..17740)
                     /gene="recO"
                     /locus_tag="PLW16_05270"
     CDS             complement(16967..17740)
                     /gene="recO"
                     /locus_tag="PLW16_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPB70351.1"
                     /translation="MHAKTTHRTAAFVLRVLDYGESDRIVTFYTEDFGKLKGIAKGAK
                     RSRRRFANALEPFSCIEILFSRRGRDGLALIEDSTPICHYASVRADLERTLIASYLID
                     LTDQFTLEGKKQADIYRLLAGFLDLVAQGLALESLWRFFELRLLKLTGYEPVLDRCIG
                     CRGLLDPNQSYQFVPREGGLKCGRCGIATYDALPISIGTIRTLLLGRDLDLAKLPRLV
                     VSPQTARESQAVLTAFIRHHLGRDLKSMQVASEIRKLCI"
     gene            complement(17825..19114)
                     /locus_tag="PLW16_05275"
     CDS             complement(17825..19114)
                     /locus_tag="PLW16_05275"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPB70352.1"
                     /translation="MKLLLTGGRILDPSCGLDTHGNLLVEDGKIAAVGERAGGKKAPG
                     KTVQILDVTGRIVVPGLVDMHTHLREPGFEYKETIATGCRAAAAGGFTAIACMPNTNP
                     VNDHRPVTEFILRKAAECGLCRVYPVAAVSLESAGKSLTEFGDLSEAGAVAFSDDGRP
                     VMDSALMRRALEYASSLGLPVISHCEDAALTAGGQMNEGAVSMELGLQGIPSTAEEIM
                     VARDLLLAGFSGAAVHIAHVSTAGSVRLIREAKARGMRVTAETAPHYFTLTEEALWEY
                     DTNLKVNPPLRRAADVTAIREALRDGTIDAIASDHAPHASTDKDVEFAYAASGMIGLE
                     TSLGLSLKLVRDGILSLEALVAKMSTHPAAILNLPGGTLRPGSLADITVIDAERNWTV
                     DIRRFQSHSRNSPFDGWTLTGKAVLTIKDGIIVFQDS"
     gene            complement(19111..20034)
                     /locus_tag="PLW16_05280"
     CDS             complement(19111..20034)
                     /locus_tag="PLW16_05280"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPB70353.1"
                     /translation="MKLERKDILGIRDLSVDEINLILDTAESFLEISTREIKKVPTLR
                     GKTVINLFYEASTRTRTSFEIAGKRLSADTINISASTSSVVKGETLIDTALTLESMHP
                     DVIVIRHSASGAPHLLAKTIRASVINAGDGTHEHPTQALLDMMTMKARKGRIAGLKVA
                     IVGDILHSRVARSNIYGLRKMGARVAVAGPATMMPLTMVEMGVEVHHRIEEAVLDADV
                     IMMLRIQTERQHQNIFPTLREYARYFCLNRRHLDLAREDCLVMHPGPINRGVEISPDI
                     ADGPASVILEQVANGVAVRMALLYLLTGGKA"
     gene            complement(20055..20594)
                     /gene="pyrR"
                     /locus_tag="PLW16_05285"
     CDS             complement(20055..20594)
                     /gene="pyrR"
                     /locus_tag="PLW16_05285"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPB70354.1"
                     /translation="METNRVVMDAEGIDRSLTRIAYEILEKNKGATDVALVGIRTGGV
                     FLAQRLQRKIAAIESRDVPLGVLDITLYRDDLDIAKKKPRLEKTDIPFSLDKMKVVLV
                     DDVLFTGRTIRAAMDALIDFGRPRFIQLAVLIDRGHRELPIRADFVGKNLPSSLWEDI
                     SVNLVEKNGRDEVVIEAAR"
     gene            20982..22244
                     /locus_tag="PLW16_05290"
     CDS             20982..22244
                     /locus_tag="PLW16_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB70355.1"
                     /translation="MAVNQESQVFLFLSQILGKKIVDTAGRPLGVLADLVVNPAESYP
                     LVTDILVSSRRNAPPLVIPWERVADCNGRFTVRPEAGGALPGRVLAENELLLRDALLD
                     KQIVDTDGAKVRRVNDLQFLKAHRCLYLVHVDVGFRGLMRRAGLVKVMDVFLRWFFDY
                     TLTDQWISWKFVQPLSTPDLLRLSITQARLSQLHPADLADIIEDLDIHKRTAVFQSLD
                     VETAAETLEETDPKIQVRLIEDMMPEKSADIIEEMSLSEAADLLGDLPKEKADGILKE
                     MEQEVAEDVKELLTHPEETAGGLMTTAFPKALPEMSAGEALDYLRREAEEMDTIHYLY
                     VTDAEDCLLGVISLREILMADPAVALKDLMDERVVAVGLDEEPDEIAEQFAKYGVTAI
                     PVVDDDDRIEGVILFKNLLEAVAPHLGK"
     gene            22270..23553
                     /locus_tag="PLW16_05295"
     CDS             22270..23553
                     /locus_tag="PLW16_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPB70356.1"
                     /translation="MSDERFPTNLRSLVLRPFQRRTWRSFAVFLMLLGPGIITSNVDN
                     DAGGITTYSLAGSEYGLAFLWTLIPIAVALIVIQEMCARMGVISGKGLSDLIRERFGA
                     RITFYLMIVLILANLGNTVAEFAGVAASLEIFGISRYVSVPAAAVFVWWLVVKGNYKT
                     VEKAFLVACLFYLSYIVSGFMGRPDWGEIGEAFITPTLRMDGAFLMMAVGLVGTTIAP
                     WMQFFLQSSIVDKGLKAENYRFARLDVIFGSITVNVVAFFIIMLCAVTLFRSGIRINS
                     AQDAALALAPLAGEYCSWLFAFGLLNASLFAASILPLSTAYTVCEAFGWESSLNCKFT
                     EAPQFYGLYALMIILGAGIILLPDVPLISIMYYSQVVNGLLLPVVLVFMLILVNDRRI
                     MGDYTNSRGMNAFSWLTVGILILLSCALVFAALFY"
     gene            23576..25009
                     /locus_tag="PLW16_05300"
     CDS             23576..25009
                     /locus_tag="PLW16_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HPB70357.1"
                     /translation="MIKTPSPSFSLRIRIEILDRPGMLGRVASAIGRSGGMIGAVDIV
                     EFRDAKIIRDLIVSAPDQETEERVVAAIRGVKGVQVLDVADRTFLMHQGGKIEVRNRF
                     PLKSRGDLAMAYTPGVARVCGAIQANPRRAYDLTLKRNTVAIVTDGSAVLGLGNIGPA
                     AALPVMEGKAMLFKEFGGVNAFPLCLNTQDPDEIVAAVKWIAPTFGGINLEDISAPRC
                     FEVEERLKAELDIPVFHDDQHGTAVVILAGLMNALTVVGKPLGEVAVVVNGLGAAGVA
                     AIHLLALAGVRTVIGCDRSGILYEGRRENMNPVKERLARRTNRERRQGTLARALEGAD
                     VFIGLSQGGVLRGRDVKRMGRDPVVFAMANPVPEIMPEEAARYARVVATGRSDYPNQI
                     NNVLCFPGLFRGVLDCKARGINDEMLIAAAHTIAATVPREELREDHIIPGVFNRTVPE
                     RVARAVRKSAVQTGMARLKHRYASFYR"
     gene            complement(25046..25237)
                     /locus_tag="PLW16_05305"
     CDS             complement(25046..25237)
                     /locus_tag="PLW16_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70358.1"
                     /translation="MGDNNTRSPEHAWKCSNCGYELQAPTPPAACPSCHRTCEFLNVT
                     CYTPDCTETGVDPRLKPAK"
     gene            complement(25381..25995)
                     /locus_tag="PLW16_05310"
     CDS             complement(25381..25995)
                     /locus_tag="PLW16_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70359.1"
                     /translation="MKDDKSRLLLFISIIIFTLLIFSAPVRSEAPAPSFKEYQSTGDY
                     FSCHIPVDWNRYEPVFGLSEEEKKIYGLTLFGPTALPAAPPTFSVHYYAPGNRLHESM
                     KKFVLVHAKPVLGFVAEGKSYGEVREIEFAGRKAQTFDRLDIRYIGERSLHPQKVSVF
                     ERFIVAPDEHHQGFYALKLSVPLDSKEEYLPIFDRLTKSFTPRK"
     gene            complement(26010..26852)
                     /locus_tag="PLW16_05315"
     CDS             complement(26010..26852)
                     /locus_tag="PLW16_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70360.1"
                     /translation="MARKKYQPDRTDFLINTEWGIPQEDLSKKEAHSSGKKLFQKRWV
                     TREEKRRLQEEHAAYSSIRNTGILLIIGSGLLLINLFFLLKSSVSAKMIWAFFELVYA
                     VALLAAGIGLFRFVCWGRNIALFIFLSFLILPFTPLLGNDKGAPVLMIIGLTGFYYLL
                     RRPARKIFSPAAAEEDSPPVRPGKSVLLWIVYAILALAVLFIYFTFDLHRAGKRAAAV
                     CQSARQGTSVEDFLANLPPENYRIIQVDDRVIIVPRAGMGRHACIVFHDGQKITGSKT
                     GFDD"
     gene            27405..28838
                     /locus_tag="PLW16_05320"
     CDS             27405..28838
                     /locus_tag="PLW16_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70361.1"
                     /translation="MKTLSKILILISFLMIILLPGSGISQQVVEYIGQGDYVMGDGET
                     PAIAESRALKLAERDAAEQAGVFIKSSTFVKDMAVIDDVIETVAKHSMSVQVIERRRT
                     PITAQNQGTGEAATLKFSVRIKATFQKLDIEKNLMMIRENSRYVDSYRKLKGEYEHQR
                     KELEDLKRKLSLADEKHRQNILKKIGAEEKRYEAVKLIEAGEVALMYNDKMSALESFT
                     SAIALHPEFAPAYGMRGAVYMELNQNEKAYADVQKAIELDPQDAFYYATRGAVTFEGC
                     SKVNPSACEQTLKDMNTAIGLQPNSPELYLMRAGVYLETNRNKEALRDLAKTNELDRT
                     AYWPAASGIAHILMIKGLKDSTNDLQGILALLTESISTVTHSKYYLDVLKPMSEAVNE
                     MREKNASADQAKEILARRSIELPDEKTSMILTDNINFVFALYAERADTYAKLGYHGKA
                     KIDLKTACRIIWKEEFCESKSPASVTD"
BASE COUNT         5430 a         8744 c         9081 g         5711 t
ORIGIN      
        1 tcggtgacga acggtccttc gcccacgcgc gtcgtgtagg ccttgcagat gccgacgacg
       61 gactgaagcg cgcccgggcc gacgcccgcg cccgcgccgg cgttgccggc cacggtgttg
      121 gaggacgtga cgtacgggta cgtgccgtga tcgatgtcca ggtggttgcc ctgggcgccc
      181 tcgaagagaa tctttttgcc cttcttgatc tccttgtaca ggatgagcga cgcatccgcc
      241 gcgtaggggc ggagccgatc cgcgtaggcc cggtattcct cgaaaatggc ggcctcgtcg
      301 acggcctcct cgccgaaata gcgggtcagc atgaagttct tctcggcgca gttggcgcgc
      361 agcttttccc ggaacgtgcc ctcgtccagg aggtcggaca ggcggatgcc cacccgggcg
      421 gccttgtctt cgtaggccgg tccgatgccc cggcccgtcg tgccgatttt cgtgccggcc
      481 ttgtgggcct cccgggccgc gtccagccgc cggtggtacg gcatgatgat gtgggcgttt
      541 tcgctgacgt agagcttcgt atgcgccgga aagagattgc ggccgttcag gctgtcgatc
      601 tcttcgatca ggaccttcgg atcgacgacg acgccgttgc caatgatgca gaacttgtga
      661 tcgtgcagga tccccgaggg gatcaggtgc aggatcgtct gctggccgtt caccaccagg
      721 gtatgcccgg cattgttgcc cccctggaaa cggacgatga catccgcgtc ttcggcgtac
      781 agatcgacga ccttgccctt gccctcgtcc ccccactggg cgcctaccac tacgacattt
      841 gccatgattc gtcctccggc ctacatttcc agtgcctgaa ccgagatcac gtgcggcagg
      901 ctccggagct tttccatgac ttcggtcggc acgacgctgt ccgtgctgag caccaccagg
      961 gcctgaccgt cgacctgctc gcggctcagg tgcatgcgcg cgatgttgat gtcatgggcc
     1021 cccagggtgg agccgatgtt gccgatgacc cccggtcggt cgatgttgta caggacgagc
     1081 atcaaaccgt ccggattgac ctcgacggtg aacttgtcga ttcggacgat gcgcgcgtcc
     1141 tggcggccaa aaatcgcccc ggccgcgcaa ctttcgccct gggcggtctt gacggacagg
     1201 gtgatcatgc tggtgtagtc cttgacctcg ctgctcttgg cctcgaccac ccggatgccg
     1261 cgctccttgg ccacgaccgg cgcattgatg tagttgacgt tctcgttcaa aatgggtgtg
     1321 agcagcccct tcagaagcga aatggtaatg ggggcgacgt tgtggttgat gatctcgccg
     1381 ctgtactcga tcttcacctg ctcgatgccg cccgtgatga gctgggcctc gaacttgccc
     1441 agtttctccg ccagtcccag gtagggctgg atgacgttga gcagctcggc gctcaccgag
     1501 gggaagttga cggcgttgcg gatctcgccc cgggtcaggt accccacgat ctgttccgcg
     1561 accgcgatgg ccacgttgat ctgggcctcg tcggtggagg cgccgaggtg gggcgtgcag
     1621 atgacgttgg gcaggccgac gaggtccttg tttttcgtcg gttcctcctc gaagacgtcg
     1681 agggcggcgc cggcgacctg accggagacg agggcgtcgt acaggtcctt ctccgtgatg
     1741 atcccgcccc gggcgcagtt gatgatgtag acgcccttct tcatcttctt gaaggcggcg
     1801 gcgttgatga tcccgcgcgt ttccttcgtc atcggggtat ggacggagat gaagtcggac
     1861 ttcgccagga gttcgtcgag tgtcaccagg gcgatcccga gattctcggc ggcctcggcg
     1921 gagatgaagg ggtcgtaggc gatgacggtc atcttgagcc cctgggcccg gtccgcgacg
     1981 atcgtgccca cccggccgat gccgatgatc ccgagggtct tgttgcacac ctccgagccc
     2041 atgaaccggt tcttctccca cttgctgtcc ttcatggagg ccgtcgcctg ggggatctgc
     2101 cgggccaggg ccatcatcat ggcgatcgcg tgctcgccgg tggtgatcgt gttgccgccc
     2161 ggggtgttca tgacgacgat gccccgtttg ctcgcggcgg caatgtcgac attgtcgagg
     2221 ccgatccccg cccggccgac gaccttcagg ttatcggcgg cggcgatgat ctccttcgtg
     2281 accttggtgg cgctccggat ggccaggccg tcgtaatcct tgatcacccc cttgagctcg
     2341 tcgggggtga ggttggtgat gacgtcgacc tcgatgccgg gggcctgcct gaatatctcc
     2401 acgccctccg gggagagggt atcgctgacc aggacgcgct tcgtcattgt ttcttccttt
     2461 ctcgtcatgc gtcggctttc tggagatacg ccttctccag cgccgccagg gccgcccgca
     2521 gatcctccgc cgcgaccgtc ggaccgcacc agaaccggaa accgggcggg gcgtccttgt
     2581 aggagttgat gtcgtaggcg atcttctgcc gggccagttc gctcgcgatt cccttcagga
     2641 acttcgcgcg ctcgtccttc ggcagggccg cgaccctggc gctcgtcacc atcaggcaaa
     2701 ccgaggtgtt ggagcggacg gtggggtcgg cggccaggaa ttccacccag tccgtttttt
     2761 gcacccattc cgatacaatc cggagattcc ccatgcttct ggcgatgagc gcgtcgagac
     2821 cgccctcggc ctccgcccat ttcaaggcgt cgaggtagtc ctcaacgcaa agcatcgacg
     2881 gcgtattgat cgtgtcgccc tcgaagatgc cggcgttgat cctgccgccc ttcttcatgc
     2941 ggaagatctt gggcatcggc caagggggat cgtacgcctc gatgcgggcg acggcccggg
     3001 gactcagaac cagcatgccg tgggccgcct cgccccccag gcacttctgc caggagaagg
     3061 tcagggcgtc gatcttctcc caggcgatcg gcatcgcgaa aacggcgctc gtggcgtcgc
     3121 agagggcgag cccttcccgg tcgtcgggga tccagtccca gtgcggcacc ttgaccccgc
     3181 tcgtcgtgcc gttggcgacg aagaccacgt cctggcgcca gtcgaccgcg ttcagatcgg
     3241 ggagcttccc gtagtccgcc ttgaggatcg tgggtttgag cttcaactgc ctggcgacgt
     3301 ccgtggccca gccgtcgctg aagctctccc agacgagcac cgtgaccggc cggggcccca
     3361 ggagcgacca catcgccgcc tcgaaggctc ccgtatcgga ggcgggcatg atcccgacga
     3421 ggtagtcggc ggggatgccc agaatcctct tcgtttcggt gatggcgcgg gtcagctttt
     3481 ccttgcccag ggcggagcgg tgcgaccgtc cgacggcggc gtcttccagg gcgccgaggg
     3541 tccaccgcgg acgcttgccg cagggaccgg aactgaaatt ggggttcata aaatcccgtc
     3601 ctctttcttg agttttcacg agcgcgcgaa tataacagga ttgcatcatt tactcaagag
     3661 attttaccga gattccggtt gacgccgacg gtgaaaatcc atatgatctt tcagccatta
     3721 ggaggggtgg ggccttgaaa aagatcaaaa cgagcttctt ctgccagagc tgcggtcacg
     3781 cgtccaccaa gtggctggga aaatgcccat cctgtggagg ctggaaccag ttcgtggaag
     3841 aggaaatccg ggaggcatcc gaggggccgt cctccgatct ccagctccac gaggccccgc
     3901 ggcccatcgc cgacatcgtc gccgatgaga aggagcgcct cgccacggga ctggccgaga
     3961 tggaccgggt tctggggggc ggcgtcgttc ccggatcggc cgttctggtc ggcggcgatc
     4021 cgggcatcgg caaatccacg ctgctgttgc aggtctgcca gcggctcgcg gagcggggcc
     4081 tcaaggtcct ctactgttcc ggggaggagt cggcccggca gatcaagctc cggggcaagc
     4141 gcatcggggc ggattcggac caactcctcg tcttcgtgga gatcgccctg gagaacatcc
     4201 tcgcggaaat ccggcggatc gagccccacg ccgtcgtgat cgattccatc cagaccgtgt
     4261 atgcctccaa tctgtcgtcg gcgccgggga gcgtggggca ggtgcgcgag gcctcggagc
     4321 gcctgatcct gcatgcaaaa aaaaccggca ttcccgtgtt tctcgtcggg cacgtcacga
     4381 aggacggatc cctggccggc ccgaaggtgc tggagcacat ggtcgatacg gtgctctact
     4441 tcgaggggga ttccgggcat gcctaccgga tcgtccgggg gatcaagaac cgcttcggcc
     4501 ccacgaacga gatcggcgtc ttcgagatgc gggacgacgg cctgaaggaa gtcgccaatc
     4561 cgtccgccct gttcctgtcg gagcgcccgg aaaaggccgc cgggtccgtg gtcgtcccca
     4621 gcctggaggg gacgcggccg atcctcgtcg agatccagtc gctggtcatt cccaccagct
     4681 tcgggatgcc ccggcgcacg accatcggcg tcgaccacaa ccgggtttcg ttgctcgtgg
     4741 cggtcatgga caaggtctgc ggccttcacc tgagcggcca cgacattttc atcaatgtgg
     4801 ccggcggggt gaaggtcgac gagcccgccg tggacctggg tctagtggcc tcgatggcgt
     4861 cgagttttct cgatcggccg gtagcgccgg gaacagtggt cttcggcgaa gtgggcctga
     4921 ccggcgaggt ccgggggatc agccagatgg agacgcgcct caaggaggcg gcgcggatgg
     4981 gctttacgcg ctgcatcctc cccgcgggaa cgccggccgg ccgcaggccg gagagcggga
     5041 tcgaaatgtt cgggatccgc tcgctcaaag acctgctcga ccccctgttc tagcaggctg
     5101 ttgaaaaacg ctcatctgcg gcgttgcgct gcaaacctca tcgctcgacg tatttctata
     5161 tacgcctcgc tcttaggttt ttgcgcgcct tgcatctgaa cctttttcaa cagcctgccc
     5221 aatccgggtg tctttcaaca ccctgctagg aaggaggaaa acgccatggt ggagaagatt
     5281 ttctggctcg gccattccag cgtccggatc cagtccggga cggtcctgta tatcgatccc
     5341 ttccagattg ccgacggtcc ggaggcggac ctcatcctga tctcccatgc gcaccatgac
     5401 catttctccc cggaggacat ccggaagatc cgcggcaagg aaaccgtgat cgtgacgaca
     5461 cccgacgttg ccgcacaact gaccggtgag gtccgtcccg tccgtccggg cgaccggctc
     5521 acggtcggtc cggtcgcgat cgaggccgtg tccgcctaca acctggacaa gccgtttcat
     5581 cccaagaaga acgattgggt cggcttcatc ctgaccgtgg acggcaagcg gatctactat
     5641 gcgggggata cggacgacgt tccggagatg caggcgctca aggcggatat cgcgatcctg
     5701 cccgtcggcg gcacctacac catgacggcg gcggaggcgg cagccgtcgc caatcgtctc
     5761 cgtccggagg cggctattcc cgtccactac ggcacgatcg tgggctccgc ggacgacgcc
     5821 cggcgctttg cggagttgtg ccgcgtcccc gtcttcatcg aaggatgagc ccccctccgg
     5881 aagcgccgct caaaccgccg gatcctactg acccctccgc gccacggaac ctgatccaga
     5941 cctttcgccc accacccgct ccgccacggg ggcggtaacc cgcgagatct ccgatgaacg
     6001 ccggcgggag acgattcccg agccgggccg ggaaaggcat caacataaaa agctatgcgt
     6061 tatttaatat ttatgttaga gagtccctca gcgtaggatt acccgctgga tggaagtgaa
     6121 atttttcaaa taatgatctt cccatcttga cattgaaggg atgatgttta ttttagcccc
     6181 caaattgaag gggataggat tgctttatca tctaaaatca taagtgacga aaggagaggt
     6241 agggagcatg aagatcagac cattacagga ccgcgtgatc gtgaagcgac tggaagagga
     6301 gcagaagacc aaggggggga tcatcattcc cgacacggcc aaggagaagc cgatggaggg
     6361 gaaggtcatt gccgtaggga aagggaagac caccgaggac ggcaagctgg tcaaaccgga
     6421 tgtcaaggcc ggggaccgga ttctcttcag caagtacgcc ggtacggagg tcaagatcga
     6481 cggggaagag cacctcatca tgagagaaga cgacatcctg ggcgtaatcg aaaaataata
     6541 atgacataag gaggataagt tcaatgggag caaagttaat acagtacgac gaagaagcca
     6601 gaaagtcgat tctcaagggg gtcaacaccc ttgccgatgc cgtcaaggtc accctcggtc
     6661 cgaagggccg gaacgtcatc ctggacaaat ccttcggtgc gccgacggtc acgaaggatg
     6721 gtgtgacggt tgccaaggaa atcgagctgg aagacaagtt cgagaacatg ggcgcccaga
     6781 tggtccgcga agtggccagc cggacgagcg acgtcgccgg tgacgggacc accaccgcga
     6841 ccattcttgc ccaggccatc tatcgcgaag gcgccaagac ggtcgccgcc ggcagcaatc
     6901 cgatggacct caagcgcggc atcgacaagg ccgtcgaggt cgtcgtcaac gagctcaaga
     6961 agcagagcaa acccaccaag gatcagaagg agatcgccca ggtcggcacc atctccgcca
     7021 acagcgacga gacgatcggc gacatcatcg ccgaggcgat gggcaaggtc ggcaaggaag
     7081 gcgtcatcac cgtggaggag gccaaggggc tcgaaacgga actggagatc gtggaaggca
     7141 tgcagttcga ccgcgggtac atctccccct atttcgtcac gaacgccgaa aagatggtcg
     7201 tggatctgga ggactgctac atcctgatca acgagaagaa gatctccggc atgaaggacc
     7261 ttctccccat cctcgagcag atcgccaaga tgagccgtcc gctcctcatc atcgccgagg
     7321 acatcgaggg cgaggccctg gccacgctcg tcgtcaacaa gctccgcggg acgctccacg
     7381 tggccgccgt caaggccccc ggattcggcg accgccgcaa ggccatgttg gaggatatcg
     7441 ccatcctgac gggcggcaag atgatctccg aagacatggg cgccaagctg gagaatgccc
     7501 agctcgcgga cctcggacgt gccaagcgca ttaccatcga caaggacaac acgacgatca
     7561 tcgacggcgc cggcgaccgc aaggcgctgg aaggccgcgt gaagcagatc cgcgcccagg
     7621 tcgaggagac gacctccgat tacgacaagg agaagctcca ggagcgtctg gctaagctcg
     7681 tcggcggcgt cgcggtgatc aaggtcggcg cggccacgga gaccgagatg aaggaaaaga
     7741 aggcccgcgt ggaggacgcc ctgaacgcga cgcgtgcggc cgtggaagag gggatcgttc
     7801 ccggcggcgg cgtagcctac ctgagaaccc tgcccgccct ggagaaactg aagctcgaag
     7861 gggacgaggc ggtcggcgtc agcatcgtcc ggaagtccct cgaggagccc atgaagatga
     7921 tcgcggccaa cgccgggctg gaaggctcca tcgtggtgga gaaagtcaag gagaagaagg
     7981 gcgccttcgg attcaacgcc cgttccgaca agtacgagga catgatcgcc gcgggcgtca
     8041 tcgatccgac caaggtgacc cggttcgccc tgcagaacgc ggcctccgtc gccggcctga
     8101 tgctgaccac ccagtgcatg atcgccgaca agccggagga caaatccgcc gccatgccgc
     8161 ccatgcctcc cggaggcggg taccccggca tggggatgta atttcccaga ccgttccatc
     8221 gttgtgaaaa cgggcccccc gggatgttgc cggggggtct tttctttggt gtgccccctt
     8281 gcattatttc caccggcggg ttagagtccg ccgacgaagg gagcgagacc atgaagggca
     8341 tcctgatccg ctggttcatt ctgacgctgg ccattcttct gtccgcctat gtcatcgacg
     8401 ggatcattgt gacgggcttc ttccccgcgc tgttcgccgc ggccgtcctg gggattctga
     8461 atgcattctt ccggcccgtc ctgctcatcc tgacccttcc cgtcaacatc gtgaccttgg
     8521 gcctcttcac gttcgtcatc aacgccctgc tgctcaagat ggcctccggc gtcatctccg
     8581 ggttcgacat ccacggcttc tgggcggccg ttctgggggc cctggtcatc agtgtcgtca
     8641 gctggttctt gaacagcttc gtcagcggcc aggggcgcat cggcccgaat gaagtcatcg
     8701 atctcaaacg ccggggtgac ggaggacgct gggaatgacc ggaaccccgg cagcgcttcc
     8761 tccggccatg cagcagtacg tgcagaccaa ggcccgctac gaggactgca tcctcctgtt
     8821 tcgcatgggg gacttctatg agatgttctt cgacgacgcc gtcacggcct cccgggtcct
     8881 cgatctcacc ctgacctccc ggaacaaggg caaggaagac agcattcccc tgtgcgggtt
     8941 tccgtaccac gccgcctcga cctatatcgc gaaactcatc gcccagggct tcaaggtcgc
     9001 catctgtgag cagatggagg accccaagct cgccaagggc gttgtcaagc gcgaggtcgt
     9061 gcgggtcgtt accccaggcc tggtggtgga ggccgacaac ctcgaggcga aggagaacaa
     9121 tttcctcgcc gccttggcgt ttgccgacgg ggcctacggg ctggccttcc tcgacatttc
     9181 caccggcgag ttccgtttgg ctgaattccg ggaccgggac gccttcctgg acgaagtcgc
     9241 atccctgtcc ttccgggaaa ttcttctgga ggaaacgttt cccgagcggg agcgcctggg
     9301 gggagggccg gagagcgaaa cgcgccgccg catcgagacc gtggccgcgg actacttcgc
     9361 gcccgccccc gcccgcgagc ttcttcgcga ccgcttttcc atcgaggcgc tccgggacct
     9421 gaacgtcgag tcctttcccg cggcgctggc cgccgccggc gccatcctcc gatatgcggt
     9481 cgaaacgcag caggaccgcc tcgggcacgt caatgcgctc cagtggtacg aatccagcca
     9541 gaatctgatc ctcgacgaca tcgcccggcg caacctggaa ctgttctcca ccatcaccga
     9601 gggccggaag gcgggctccc tctatcatgt cctcgatgaa accatgactg ccatgggcgg
     9661 ccggcgcctg cgctggtggc tgagctaccc tctgtgtcag ccggagcgga tccggaaacg
     9721 actcgccgcg gtcgccgacc tcaaggaaca ccacctgctc cgccagaatc tccgccgggc
     9781 gctggggcgg cagcacgacc tggagcggct gggcggccgc atcgccatgg gggtggccaa
     9841 cgcccgcgac ctcgtggccc tgaagcagac cctgcaggcg ttgccggagg tcaagggtct
     9901 cttggcggat ctgtcggcgc ccctgctggt cgccattcgg gaggggatcg acgagatgtc
     9961 ggatgtcctg gcgctggcgg agtccgccat cgccgacgat ccccccctga gcctccggga
    10021 cgggggactg atccgggagg gattcgacgc agagctggac cggctcacga gcatcggtcg
    10081 cgacggacgg aaatggattg ccgccctgga ggaacgcgag cgccgccgaa cggggatcgg
    10141 cagcctcaag gtcgcctaca acagcgtttt cgggtactac atcgagatca ccaaggccaa
    10201 tgccgaccac gtcccggacg actacatccg caaacagacc ctggtcaacg ccgaacgcta
    10261 catcaaccag gagctcaagg agtacgaata cacggttctc aacgccgacg acgcccggcg
    10321 ccagcgggag tacgacctgt tcgtcgatgt ccgggaacgg atggcgggcc agatccggcg
    10381 gatgcagaaa acggcggggc tgctggccga tctggatgcc ctggcggcgc tcgccgaggt
    10441 tgcggagcgg tacggctaca cctgtcccgt cgtggacgac gaggacgtga tcgagatccg
    10501 ggacgggcgg cacccggtcg tggagcggat gccgctgcct gaggggtttg tccccaacga
    10561 cgtcctgctc gacctggacc ggaaccgcct gctggtcatc acgggaccca acatggccgg
    10621 gaagtccacc tacatccgcc aggtggccct gattgtcctg atggcccaga tgggcagctt
    10681 cgtccccgcc gcgtcggccc ggatcggctg cgcggaccgg atcttcaccc gggtgggggc
    10741 ggcggacagc cttgcccgcg ggcagagcac cttcatggtc gagatgacgg aagtggcgaa
    10801 catcctttca cgtgcgacat cccgcagcct ggtgatcctc gacgaagtgg gacgtgggac
    10861 cagcacgttc gacgggctca gcatcgcctg ggcggtggcg gagcgcctcc acgacgcggc
    10921 ccctcggggt gtccggtcgc tgtttgccac ccattaccac caactgacgg agctggcgac
    10981 caccaaggcc ggcgtcgcca attacaacat cgccgtcaag gaatggggca gccggatcat
    11041 ctttctgagg aagatcaagg agggcgggac gaaccgcagc tacggcatcc aggtggcacg
    11101 gatcgccggc gttcccgagg aggtgatcag ccgggcgaag gagatcctcg acaatctgga
    11161 gaagggggag ttggacccga cgggaaagcc gaagctggcg cgcggccggc ggccagcgga
    11221 gcggaacccg gatcagctgg gtctgttcat gggagaggag gagcgggtgc tccaggagct
    11281 ccgcgatgcc gacatcccga acctgacgcc ggtggaggcc ctgaatcgcc tgaacgaatg
    11341 gaagaagcgg ctggggaagg gctgaaggga aacgggattc cgccgtgggc ctttcgccct
    11401 ttgcctattt gatacacacc ctccggacct gcttgaagtc cgtgagtccc tggatggatt
    11461 tctggatgcc gtcctgaagc agggtcgtca tgccctcggt catcgctacg tcccgcatct
    11521 cctcgatggt cgcgtgtttc tgaatgagtt tcttgatgtc gtccgtgccg gccaggagtt
    11581 catggatgcc catccgcccc ttgtaccccg tcttgtcgca tgcgtcgcag cctttgggac
    11641 ggtagagggt caggtcatcc gtgtagggga cgttgagctt cgcgaaggcc tccggaccgt
    11701 agctctccac catctcctcg tattcctgcg gcgtcgggtg gtaggcctcc ttgcacttct
    11761 tgcacagggt ccggacgaga cgctgggcca ggatgcctag gagggcgtcg gcgaagttca
    11821 gggggtcgat gcccatgtcg agcagacggg tgatcgtttc cggggcgctg ttcgtgtgca
    11881 gggtgctgaa aacgagatgg ccggtgagcg acgcctcgac gcccgttttc gccgtttcga
    11941 aatcgcgcat ttccccgacc atgatcacgt ccgggtcggc ccggagaaag gcccgcatgg
    12001 cggcggcgaa gtcgaagccg atcttcggct gaacctgaac ctgacgcagt ccatactggg
    12061 tgatttcgac ggggtcctcg gcggtccaga tcttccggtc cggggtgttg atgtgatgca
    12121 gcgccgcgtg gagggtggtt gtcttcccgg agccggtcgg cccgacgacg aggaccatgc
    12181 cgtagggctt ttccagaatc ttcagaagct cgccgtaatt gcgcgggagc atccccatgg
    12241 cctcaagcgg cagggtttcc cccttggcca ggatgcgcat gaccacgtct tccacgcccc
    12301 cctgggtcgg gatggtcgcc acgcggagtt cgatttcgtc tccccccgtg cgcttgaatt
    12361 tgatcttccc gtcctgcggt agacgcttga ccgtgatgtc caggttggac atgatcttga
    12421 tccgggagat gatcgcggac cggtaactgt agggcagcgt ctggtagagg gcgcaatcgc
    12481 cgtcgacccg gtagcggatc tcgacgttct tgcggccgac gttcggctcg atgtgaatgt
    12541 cggagcaacg gcggatgtag gcgtcgttga tgatcttgtt cacaagttgc atgatgacgc
    12601 tgtccgactc ggtgacgacg tcgccgctgt catcttcttc gtccggcgcc tcgtccgcct
    12661 ccagctttcc cagaagatcc gagatggacg attcgtcctc gggggtctgg aagaagtagt
    12721 tgatgaactt gacgatgtcc tcggccaggg cgacgtcgta cttgaccgcc ttggtccgca
    12781 ggaggttttc gatcatgtcc cgcttcagga tgttgttcgg atcgtcgacg atgacctgga
    12841 tgttgccgtc caccttgctg atggggaccc agagttcgcg ccggagatag tcccgcttca
    12901 ggttcttcag gagatctccg gggatgggga tcttgtcgct gaactggatg aacttgcagc
    12961 ggaagaactc ctcgaaggat tttcccagat cttccttgga gatcttgtgc ttcctcatca
    13021 ggaaggtctc catggtttcc ttctgctcgc gcgattcctc ccaggcgttt tcgagctcct
    13081 cctccttcag caggtcccgg ctgatcaggt agtcgaagcg cgttttgcgc cgccgggcga
    13141 accgctcctg gttatagaag gcgaccccca ggaccgccgc aatttcctgg agaaagtccg
    13201 tttcctcgac ggagaagggg cctcccccct ttttgttcag gatctggacg acgcccatca
    13261 gggtctggtt atgaaagatc ggagcggcca ggatctgctt cgtccggaat ccggatttct
    13321 tgtcccagct cagatcgaac gacaggtcct tgtcgatctg cttgagcgcc tcgacgtcgt
    13381 aggcgtctgc gatgttgacc acgcggccgt tgttggccac gaagcccgca atgctccggt
    13441 tgttgatcgg gacgcggatc tcccgcacgg acgaccccac gagaaacatg gagtagatct
    13501 cgttcttggg acgatccacc acatagatcg tgatggagtg ggcgtcgaac aggttgagaa
    13561 tcccgtcctt caggtcgacc aggatctgct tcgtgttctg cgccgcatgg atgcgattgg
    13621 tgatgtcctg caacgccttg cggatctgaa gcttttcttc cagtgcgctg atctgctgat
    13681 ttttggggct ggcgtccata aatcttcgag ctccaagaag cgaatctgcc cggtttcccg
    13741 acagggatcg tgaccgcaag gttttttcaa ctgagatgca ggctaatgca gattgcaaaa
    13801 taattcaatc aaaacttgcg cctgctcccg tcgccgacgt tgcaccccga aacgcgtgtg
    13861 tacacccggg ctgaagccca cccgttacac gctcgtgacg atcttggcga aatccgcgac
    13921 ccggtgaaac agggcggaaa tctccgccag gagcgccagg cggttgttac ggatcgcctc
    13981 ctccttggcc atgacgagca cggtctcgaa aaaggcatcc acggactgcc ggattcccgc
    14041 catttcaacc agggcggacg ggtagtcctc cgcgtccaga tgggtgaggg cgcgcgtccg
    14101 gatctcccgg aaggattggt agagggtccg ctcctcagcc gtttccagca ggtcctcgcg
    14161 gacggaaccg cctggaaaat ccttgaggat gttcccgacc cgcttgaagg cgatcgccag
    14221 aggctcgaag ttctcgtgat ccttgaaggc ctccatggcc tcgattttct tgacggtgcg
    14281 gacgaggtcg gagatgcccg tggccagaac ggcgtcgacc acgtcgtagg ggcgcccctg
    14341 ggcgatgagc tggttttcca ggcggccccg gaagaagtcc aggatgtcgg cgcgggtttc
    14401 ccccgcgggc cgcttcgctt tttcctccag caggctcaag ccgagatcga cgagctcgtc
    14461 gaggcggatc ggataccgct tggcgaggag gatgttgatg atgcccaggg cctgacgccg
    14521 cagggcgtag ggatcggccg ttccggtcgg gatcagcttg acggcgaaga atccggcgat
    14581 cgtgtccatc ttgtcggcga tgctgacgat cgccccttcg tcggtggcgg gcagggcccc
    14641 gcccgcctgc gtggggaggt agtgctcgta gatggcccgg gcaacggtgg ggttttcccc
    14701 ggcgagcagc gcatattccc gccccataac cccctggagc tcggagaatt cccccaccat
    14761 ctgggtgtcc agatcggcct tcgccagcag cgcggtgcgc tcgacggtgg ccttcagggt
    14821 cggatcgatg cggtccgcca gggtgagagc gagggccctg aagcgcatga ccttctcata
    14881 ggacgtgccc agctgggtgt gataaacgac ctgtttcagt ccctcgacgc gctccgtcag
    14941 agggaccttc tggtcctcgg tgaagaagaa atgcgcgtcc gagaggcgtg ctcggatgac
    15001 cttttcgttc ccccgggcga cgaccgacgg atcccgggcc agcgtgttgt tgacggcgag
    15061 gaaatggggg aggagacgtc cctccgcgtc gacgaccgga aagtatttct gatgggagat
    15121 catggacgtc accaggacct cttggggaag tcgcaggtag tcccggtcga aactgccgca
    15181 tacgactgtc ggatactcgg tcagaaagac cacctgctcc aggagcgtct catcctccag
    15241 cgcgcgtccc gatacctggg ccgccgcctt gcgggtctcc tgacggataa tagccttccg
    15301 ttccgcgggg tcgacgatca cgcaccgctc cttcgttcgg gcgaggtaat cggcgaaatc
    15361 tttaacggag accgcttcgg ggctcatgaa gcgatggccc cgcgtcaggt tgccgctctg
    15421 gatatttccg agcgtgaagg gaacgacgcg gccgccgtag agggcgagca gccagtggat
    15481 cggacgggcg aaacggatgt ccagatcgga ccagcgcatg gatttcttga aggggagccc
    15541 caggatgagg cgtggaagga tctccggcag gagggcttcc gtcggctcgc cggcgatggt
    15601 tttgcgcacg cacaaatact cccccttctc ggtgacggcc gtgcccacgt cacccagttc
    15661 caccccctgg ccccgggcaa aaccgagcgc ggccttggtc ggctggccct gtgcgtcgaa
    15721 ggcggccttc ttcgccggtc ccatcttttc gacctcgaga tcctcctgcc ccccggccag
    15781 gttggcgacg accagacaga ggcggcgggg cgtcgccagc gactggatcg cgccgtgggc
    15841 gagacggcag gccgagaatt cccggcggga catctcttcg aggtctcgca tcgccttcgg
    15901 cagaaacgcc gcggggattt cttccgttcc tatttccagt aagagttcct tacccatgaa
    15961 tcgatctccc gatgatgtcc aggggtcatc cccgatcgag caggggatac cccagccttt
    16021 cccgttgtct gagccagcct tcggcgctca gccgggctag gttgcggacg cgcccgatgt
    16081 agctcgtcct ctccgccacg ctgatcgccc cccgggcgtt cagcagattg aaggtatgcg
    16141 agcacttcag gcaatggtcg taggtcggca ggaccaggtc cttttcggcc atgcggatgc
    16201 cttcggcctc gtccatgtcg aaacgtttgc ggagcatctc cgcgtccgcg tactggaagt
    16261 tgtaggtgga ccactccacc tcgccctggt gatggacgtc tccatacttg acggtatcgt
    16321 tccacttcag gtcatagacg ttgtcgatgc cttggatgta catggcgatc cgctcgatgc
    16381 cgtaggtcag ctcgacgctg atcggcttga ggtctactcc gcccacctgc tgaaaatacg
    16441 tgaactggga gatctccatg ccgtccagcc agacctccca gcccaacccc caggccccga
    16501 cggtggggga ttcccagtcg tcctcgacga agcggatgtc gtgttcgagg gggtcgatcc
    16561 cgaagctctt gagggagttc aggtagcgct cctggatgtc gacgggagag ggcttcatga
    16621 tcacctggta ttggtaatag tgctgaagcc ggttcgggtt ctccccgtag cggccgtccg
    16681 tcgggcgccg cgacggctcg acatacgcca cgttccaggg ttccggtccc agggtccgga
    16741 gaaacgtcgc ggggttgaag gtgcccgccc cgacctccag gtcgtagggc tgctggatga
    16801 cacagccctg cgaggcccag tagcgttcca gggcaaagat gagttcctga aaggtcaatg
    16861 ccatgcgtcc tcctcgaaag cgtcccgccg cccggcggcg ggcccgcaat tttccggtgc
    16921 gttcctaaca cggcggcccg aacctgtcaa tatgaaagca atccggtcag atgcagagtt
    16981 tccggatctc gctcgcaacc tgcatggatt tgaggtcccg ccccaggtgg tgacggatga
    17041 aggcggtcag gaccgcctga ctttcgcggg ccgtttgtgg ggagacgacc aagcggggga
    17101 gttttgcgag gtccaggtcc ctgcccagaa gcagggtccg gatggtgccg atcgagatgg
    17161 ggagggcgtc gtaggtcgct atcccgcagc gtccgcattt cagcccccct tcccggggga
    17221 cgaattggta ggattggttt gggtccagga gaccccggca gccgatacag cggtccagga
    17281 ccggttcgta accggtcagc ttcagcagcc tcagttcgaa gaatcgccaa aggctctcca
    17341 aggccaggcc ctgcgccacc aggtcgagaa accccgcgag cagccggtag atgtccgcct
    17401 gcttcttccc ttccagggtg aactggtccg tcagatcgat gagataggag gcgatcaggg
    17461 tgcgctccag gtccgcgcgg acggacgcgt aatggcagat cggggtggag tcctcgatca
    17521 gggccaggcc gtcccgtccc cgccgcgaga acaggatctc gatacaggag aagggctcca
    17581 gggcgttcgc gaagcgccgc cggctgcgct tcgccccctt ggcgatcccc ttcagcttac
    17641 cgaagtcctc ggtgtagaag gtgacgatcc gatccgattc gccgtagtcc aggacgcgca
    17701 ggacaaaggc cgcggtccgg tgggtggttt tggcatgcat catgactcat cgggcgcggg
    17761 gtttcgagca tgggggaaaa gggatatctt cccgaggccc ggggcccgac gcctcaaacc
    17821 cgtctcaaga atcctgaaaa acgatgatcc cgtccttgat cgtcagaacg gccttgcccg
    17881 tcagggtcca cccgtcgaag ggggaattcc ggctgtggga ctggaaacgg cggatgtcga
    17941 ccgtccagtt ccgctcggca tcgatgacgg tgatgtcggc caggctgccg ggccggagtg
    18001 tgccgcccgg gaggttcagg atggcggcgg gatgcgtgct cattttagcc accagcgcct
    18061 ccagcgacag aatgccgtcc cggaccagtt tcaggctgag gccgagggag gtctccagac
    18121 cgatcatgcc gctggcggca taggcgaatt cgacgtcctt gtccgtggag gcgtgggggg
    18181 cgtgatcgct ggcgatcgcg tcgatcgtcc cgtcccggag cgcctcccgg atggcggtga
    18241 cgtcggcggc acgccggagc ggcgggttca ccttcagatt cgtgtcgtat tcccagagcg
    18301 cctcctcggt cagcgtgaag tagtgggggg ccgtctccgc cgtcacgcgc atgccgcggg
    18361 ccttggcctc ccggatgagg cggacggacc cggccgtgct gacgtgggcg atgtggacgg
    18421 cggcgcccga gaagccggcg aggaggaggt cccgggcgac catgatctct tccgcggtgc
    18481 tcgggatgcc ctgaagaccg agctccatcg agacggcgcc ctcgttcatc tggcccccgg
    18541 ccgtgagagc ggcgtcctcg cagtgtgaga tgaccggcag gccgagcgaa gacgcatact
    18601 ccagggcgcg ccgcatgagc gcgctgtcca tcactggcct gccgtcgtcc gagaaggcga
    18661 cggcgccggc ctccgacaga tcgccgaatt cggtcaggct ctttcccgcc gactccaggc
    18721 tgacggcggc cacgggatag acgcggcaga ggccgcactc cgcggccttc ctcaggatga
    18781 attccgtgac ggggcggtgg tcgttgaccg gattcgtgtt cggcatgcag gcgatggccg
    18841 tgaagccccc ggcggcggcg gcccggcagc cggtggcgat ggtctccttg tactcgaagc
    18901 cgggctcccg caggtgggtg tgcatatcga cgaggcccgg gacgacgatc cggcccgtca
    18961 cgtccaggat ctgaacggtc tttcccgggg ccttctttcc gccagcgcgc tccccgacgg
    19021 ccgcgatctt tccgtcctcc acgaggaggt ttccgtgcgt atccaagccg caggaggggt
    19081 cgaggatccg ccctccggta agaagcagct tcatgccttc cctcccgtaa gcagatagag
    19141 gagcgccata cggacggcga cgccgttggc gacctgttcc aggatcaccg atgccgggcc
    19201 gtcggcgatg tccggcgaga tttccacgcc ccggttgatc gggcccggat gcatgacgag
    19261 gcagtcttcg cgggccagat ccaggtgccg gcggttcaga cagaagtaac gggcgtattc
    19321 ccggagcgtc ggaaagatgt tctgatgctg gcgctccgtc tggatgcgga gcatcatgat
    19381 gacgtcggcg tcgaggacgg cctcctcgat ccggtgatgc acctcgaccc ccatctccac
    19441 catggtcagc ggcatcatcg tggccggtcc ggcgacggcg acccgcgctc ccattttccg
    19501 gagtccgtag atgtttgacc gggccacccg gctgtgcagg atgtctccca cgatggccac
    19561 cttgagtccg gcgatccggc ccttgcgggc cttcatcgtc atcatgtcca gaagggcctg
    19621 ggtcggatgc tcatgcgtgc cgtcccccgc gttgatcacc gaggcccgga tcgtcttcgc
    19681 caggagatgc ggggcgccgg aggcgctatg gcggatgacg atgacatcgg ggtgcatgga
    19741 ttctagggtg agcgccgtat cgatcagggt ctcgcccttg acgacgctgc tcgtcgaggc
    19801 ggagatgttg atcgtatccg cgctgaggcg cttgccggcg atttcgaacg atgtccgggt
    19861 gcgggtgctc gcctcgtaga agaggttgat caccgttttc ccccgcagcg tcggcacctt
    19921 ctttatttcc cgggtggaga tctccaggaa ggattccgcg gtatcgagga ttagattgat
    19981 ctcgtcgacg ctgaggtccc ggatgcccag aatgtctttt cgctccagtt tcatgggaag
    20041 ccccttcctt ctcgtcaacg cgcggcttcg atgaccacct catcccggcc gttcttttcc
    20101 acgaggttca cgctgatgtc ctcccagagg gacgaaggca ggttcttacc gacgaagtcc
    20161 gcccggatgg ggagttcccg gtgcccccgg tcgatcagga ccgccagctg gatgaagcgc
    20221 ggccgcccga agtcgatcaa cgcgtccatg gccgcccgga tcgtccggcc ggtgaacagg
    20281 acgtcgtcca ccagaacgac cttcatcttg tccagggaaa agggaatgtc ggttttttcc
    20341 aggcggggtt tcttcttggc gatatccagg tcgtcccggt agagggtgat gtcgagcacg
    20401 cccaggggaa cgtcccggga ttcgatggcg gcgatctttc tttgcaggcg ctgggccagg
    20461 aacacgccgc ccgtccggat gccgaccagg gccacatccg tggcgccctt gtttttttcc
    20521 aggatttcat aagcgatgcg cgtcagggag cggtcgattc cctccgcatc catcaccacc
    20581 cggttcgttt ccatggctcg ctcccccgct gtcttgcggc cccgaggggc cgacggcaaa
    20641 aaaaaagccc cccaccgtgt gggaaggcct tatcgccgcc gcttgtcgat caactgattc
    20701 atggtttctt tcctttttcg atctcgccgg atcaaattaa aagggcgtcc ttgattgcgg
    20761 tttatagcgg aagggcgtcg tcgtgtcaag acaaaaaccg gcaggcccgg gacagcagga
    20821 gggacatccc atcgcgattt ctgaaaacct tccccccgct gtcgggagcg cggccggccc
    20881 gtcgtgcggg aacgtccttt cgaaagggcg gcggggagaa aaaaagcttt tgctatctta
    20941 ccgcaagtgc tatagaagat ccgcctggag gcgcttgaac aatggccgtc aatcaagaat
    21001 cccaggtttt tttgtttctg agccagatcc tgggaaagaa aatcgtcgat acggcggggc
    21061 gcccgttggg cgttctcgcc gatctcgtcg tgaacccggc ggaatcctat ccgctggtga
    21121 cggacatcct cgtctcatcc cgccggaacg ccccccccct ggtgattccc tgggaacggg
    21181 tcgccgactg caacgggcgg ttcacggtcc ggcccgaagc gggcggcgct ttgcccggcc
    21241 gggtgctcgc cgagaacgaa ctcctcctgc gcgacgcgct gctcgacaag cagatcgttg
    21301 acaccgatgg ggcgaaggtg cggcgcgtca acgacctcca gtttctcaag gcccaccggt
    21361 gcctgtacct cgtccacgtg gatgtcggtt tccgcgggct catgcggcgt gccggtctcg
    21421 tgaaggtcat ggacgtcttc ctgaggtggt tcttcgatta caccctgacc gaccagtgga
    21481 tttcatggaa attcgtccag cccctgtcca cgccggacct gctgcgcctg agcatcaccc
    21541 aggcccgcct ctcccagctc cacccggcgg atctcgccga catcattgag gatctcgaca
    21601 tccacaagcg gacggccgtg ttccagtccc tcgacgtgga gacggcggcg gagaccctgg
    21661 aggagaccga tcccaagatc caggtccgcc tcatcgaaga catgatgccc gagaagtccg
    21721 ccgatatcat cgaggagatg tccctgagcg aagcggcgga tcttttgggc gacctcccca
    21781 aggagaaggc cgatggcatc ctgaaggaga tggaacagga ggtcgcggag gatgtgaagg
    21841 aactcctgac gcatcccgag gagacggccg gcgggttgat gaccacggct ttccccaaag
    21901 ccctgccgga gatgagcgcg ggggaggccc tggactacct gaggcgggag gcggaggaga
    21961 tggacaccat ccattacctc tacgtcaccg atgccgagga ttgcctgctg ggcgtgatca
    22021 gcctccgcga gatcctgatg gccgatcccg cggtggcgct caaggacctg atggatgaac
    22081 gggttgtagc ggtcggtctc gacgaggagc cggacgagat cgccgagcag ttcgccaagt
    22141 acggcgtgac ggccattccg gtcgtggacg acgacgatcg tatcgagggc gtgatcctct
    22201 tcaagaacct tctcgaagcg gtagcgcccc acctggggaa gtgagcgcga tggaagagga
    22261 tggccacaca tgtccgacga acggttcccg acgaacctcc gcagtctggt cctgcgcccc
    22321 ttccagcggc ggacctggcg ctcgttcgcc gtttttctca tgctgctggg ccccggcatc
    22381 atcacgtcca acgtggacaa cgatgcgggc ggcatcacga cctattccct ggccgggtcc
    22441 gagtacggcc ttgccttcct gtggaccctg atcccgatcg ccgtcgccct gatcgtcatc
    22501 caggagatgt gcgcccggat gggcgtgatc tccggcaagg ggctctccga cctcatccgg
    22561 gaacgcttcg gcgcccggat caccttctac ctcatgatcg ttcttatcct ggccaacctc
    22621 ggcaacaccg tcgccgagtt cgccggggtg gcggccagcc tggagatctt cgggatttcg
    22681 cgctacgttt ccgtccccgc ggcggccgtc ttcgtctggt ggctcgtggt taagggaaac
    22741 tacaagacgg tcgagaaggc cttcctggtc gcgtgcctgt tctacctgtc ctacatcgtt
    22801 tccggattca tggggcggcc cgactggggc gagatcggcg aggccttcat cacgccgacg
    22861 ctccggatgg acggggcgtt tctcatgatg gccgtcggcc tcgtcgggac gaccatcgcg
    22921 ccctggatgc agttcttcct gcagtcctcc atcgtggaca aggggctcaa ggccgagaat
    22981 tatcgctttg cccgcctgga tgtgattttc gggtcgatta cggtcaacgt cgtggcgttc
    23041 ttcatcatca tgctgtgcgc cgtcaccctg ttcagatcgg gcatcaggat caactccgcc
    23101 caggatgccg ccctcgccct ggcgcccctg gccggggaat actgctcgtg gctgttcgcc
    23161 ttcgggctcc tgaacgcgtc gctcttcgcc gcctcgatcc tgcccctgtc cacggcctac
    23221 accgtctgcg aggccttcgg ctgggaatcg tccctgaact gcaagttcac cgaggcgccg
    23281 cagttctacg ggctgtacgc gctcatgatc attctggggg cgggcatcat cctcctgccg
    23341 gacgttccgc tcatctccat catgtactac tcccaggtcg tgaacgggct gctgctgccg
    23401 gtcgtgcttg tcttcatgct catcctggtc aacgaccgca ggatcatggg cgactacacc
    23461 aacagccggg gcatgaacgc cttctcctgg ctgaccgtcg ggatcctgat cctgctctcc
    23521 tgcgccctgg tgttcgccgc ccttttttac tgatcgggat cattggaggg gtgtgatgat
    23581 caaaacaccg tcgcccagct tcagtctccg gatccgcatc gagatcctcg accggcccgg
    23641 catgctgggc cgggtcgcct ccgcgatcgg ccggtctggg ggaatgatcg gggccgtgga
    23701 tatcgtggag ttccgggacg ccaaaatcat ccgcgacctg atcgtgtcgg caccggacca
    23761 ggaaacggag gaacgggtgg tcgcggccat ccggggcgtc aagggggtgc aggtcctgga
    23821 cgtggcggac cgcacctttc tcatgcacca ggggggaaag atcgaagtcc ggaaccgctt
    23881 tcccctgaag agccggggcg acctggcgat ggcctacacg cccggcgtcg cccgggtctg
    23941 cggggcgatc caggcgaacc cccgccgtgc ctacgacctc accctcaagc ggaataccgt
    24001 ggccatcgtc acggacgggt cggccgtcct ggggctcggg aacatcgggc ccgccgcggc
    24061 cctgccggtc atggaaggca aggccatgct gttcaaggaa ttcggcgggg tgaacgcctt
    24121 tcccctttgt ctcaacaccc aggatcccga tgagatcgtc gccgccgtga agtggatcgc
    24181 gccgaccttc ggcggcatca acctggagga catttccgcc ccccggtgtt tcgaagtcga
    24241 ggagcgcttg aaggccgaac tcgacattcc cgtcttccac gacgaccagc acggcacggc
    24301 tgtcgtgatc ctggcgggtc tcatgaacgc cctgaccgtc gtcggcaagc ccctcgggga
    24361 ggttgctgtc gtggtcaacg gcctgggcgc ggccggtgtg gccgccatcc atctccttgc
    24421 cctcgcgggc gttcggacgg tcatcggctg cgatcgttcc ggtatcctct acgaggggcg
    24481 gcgcgagaac atgaatcccg tcaaggagcg tctggctcgc cggacgaacc gggagcggcg
    24541 gcaggggacg ctggcccggg cgctggaagg ggcggacgtc ttcatcggcc tgtcccaggg
    24601 gggggtgttg aggggaaggg acgtcaagcg gatgggacgg gatcccgtcg ttttcgcgat
    24661 ggccaatccg gttcccgaga tcatgcccga ggaggccgcc cggtacgccc gggtcgtcgc
    24721 caccgggcgg tccgactatc caaaccagat caacaacgtc ctgtgtttcc ccgggctctt
    24781 ccgcggggtc ctggattgca aggcgcgggg gatcaacgac gaaatgctca tcgccgccgc
    24841 gcacaccatt gccgccacgg taccgcggga ggaactccgc gaggatcaca tcattcccgg
    24901 cgtcttcaac cggaccgtcc cggaacgggt cgcccgggcc gtccgcaaga gtgccgtgca
    24961 aaccggcatg gcccggctca agcaccgcta cgcctcgttc taccggtagc gggaggcgac
    25021 cgtcccgtca cggggcggga cgcgtctatt tcgccggctt gagccgcgga tcgacgccgg
    25081 tctccgtgca gtccggcgtg tagcaggtga cgttcagaaa ttcacatgtc cggtggcagg
    25141 acggacacgc cgccggcggg gtcggcgctt gaagttcgta gccgcagttg gagcacttcc
    25201 aggcatgttc cggtgatcgg gtgttgttgt ctcccatcgc atgacctccc tatgtccgat
    25261 gatcataccc tcttaggcca tgccgctccc ggcgtcaagc cccgggatgc cccggccggg
    25321 cgtctttctc ccaggaacgg gtgcatcacc tgaatcagga tgggaaggtc gttccggaat
    25381 ttacttccgg ggagtgaatg attttgtaag tctatcgaaa ataggcagat actcctcttt
    25441 agaatcaaga gggacggaca attttaaggc atagaatccc tgatggtgtt catcgggagc
    25501 cacaataaat ctttcgaaaa cggatacctt ctgcggatgc agggatcgtt cgccgatgta
    25561 acggatatcg agccggtcga acgtttgggc ctttctgccg gcaaattcga tttcccggac
    25621 ttcaccgtag gattttcctt cggcgacaaa tcccagaacc ggtttggcat ggacaagaac
    25681 gaactttttc atggactcat gcagccggtt tcccggagcg tagtagtgga cggaaaaggt
    25741 cggtggagcc gcgggaaggg cggtggggcc gaagagggtc aggccgtaaa tctttttttc
    25801 ttcttcggat aaaccgaaaa ccggttcgta tctgttccag tccaccggaa tatgacagct
    25861 aaagtaatcg ccggtggatt gatattcttt aaacgaaggc gccggcgctt ctgaccggac
    25921 aggcgctgaa aagatcagca gcgtaaaaat gatgatggag atgaacaaca gcaaacggct
    25981 tttatcgtcc ttcatcggat cattcctcct cagtcgtcaa atccggtttt gctccccgta
    26041 attttttgcc catcatggaa aacgatgcag gcatggcgtc ccatgcctgc tctgggaacg
    26101 atgatcacgc gatcatcgac ctggatgatc cggtaatttt ccggcggcaa attggccaga
    26161 aaatcttcca cggatgtccc ctgtcgagcg ctttgacaga cggcagcagc cctctttccc
    26221 gcccgatgca gatcaaaggt aaaataaatg aacagaacag ccaaagcaag tattgcgtaa
    26281 acaatccaca atagaacgga ctttcccggc cggacagggg gactgtcttc ttctgctgcc
    26341 gcgggcgaaa atatcttgcg cgccggacgg cgcagcagat agtagaaacc ggtcaaaccg
    26401 atgatcatca gtaccggcgc ccctttgtcg ttaccgagca ggggagtaaa gggcaaaatc
    26461 agaaaagaaa ggaagataaa caaggcgatg ttccggcccc agcagacgaa ccggaacagt
    26521 ccgattcctg cggccaaaag agcaacggca tagacaagtt caaaaaatgc ccaaatcatt
    26581 ttggcggaga cagaactctt gagaaggaaa aagagattga tcaggagcaa cccactgccg
    26641 atgatgagca gaattccggt gttgcggatg gacgaatatg cggcatgctc ctcctggagc
    26701 cttcttttct cttccctggt gacccagcgt ttctgaaaaa gctttttccc gctgctgtgc
    26761 gcttcttttt tggataaatc ttcttggggg attccccatt cggtattgat cagaaaatcg
    26821 gttctgtcgg gttgatactt tttccgggcc atagctgctg tcaagcctca atgtgctgtt
    26881 tggctccgtc gatcgatgga gaaacaaaaa agttgaagat agccggaatc gccgcaaaca
    26941 ggcccgcatt agaccagttt ttgcagacag aagcaaaagc ttttaaaagg cagaaaaaag
    27001 acctcaacga ggccgggggc gttcgcgaac gggcatagcc acctcctgaa aacgcgtaat
    27061 tttcgttttc cctcggcgaa ggcttccctg tcgcctaaag aggtcgtttc cgtgtcctct
    27121 ttcttcggtt gcggccctat aaatcccccc ctccttctgg aggtggtgtt cggtaacgcc
    27181 tgtttttggg tcaacgatcc ttttcgatcc cctgttcggt aacatttaat ttgaggcaat
    27241 tcgctaacaa accggacaat tttgttttct cttgaaattg tggaaaaata ttcttgacgc
    27301 agaagtgaac gctccttatg cttccacaag ttaataaggg agttcttatc aatttaccaa
    27361 tcattcatac ttatggcttc accctggaat gaaggagcct tttcatgaaa acactctcaa
    27421 agattttgat tttgatttcc ttcctaatga ttatactgct gccgggttca ggaattagcc
    27481 agcaggtagt ggaatacatc gggcaaggtg attatgtaat gggcgacggc gaaacgccgg
    27541 ctatcgccga atcgagggct cttaagcttg ccgaaaggga cgctgccgag caagcaggcg
    27601 tattcatcaa gagttccaca tttgttaaag acatggcggt gatcgatgac gtcattgaga
    27661 cggttgcgaa gcactcgatg tcagtgcagg tgatagagag gaggaggacc cccatcaccg
    27721 cacaaaacca gggtactggg gaagccgcaa cattgaaatt ctccgtaagg ataaaagcca
    27781 ccttccaaaa actggatatt gagaagaacc tcatgatgat cagggagaat agccggtatg
    27841 tagattctta ccgaaaattg aaaggggaat atgaacatca gagaaaggaa ctggaagacc
    27901 tcaaaagaaa attgtccctc gcagatgaaa aacatcgtca gaatattctt aaaaagatcg
    27961 gtgcggagga gaaacgttac gaagcggtta agctgatcga ggccggtgaa gtggctctca
    28021 tgtacaatga taagatgtct gccttggaat ctttcacgtc cgctatcgcc cttcatccag
    28081 aattcgctcc tgcctacggt atgcgaggtg ccgtatacat ggagctgaat caaaatgaaa
    28141 aggcttatgc ggatgtgcag aaggcaatcg agctcgatcc ccaagatgcc ttttattatg
    28201 ccacacgcgg ggcggttacc ttcgaaggat gctccaaggt gaatccatct gcctgcgaac
    28261 aaaccttgaa agatatgaac accgccatag gccttcagcc aaactccccc gagctctacc
    28321 taatgagggc cggcgtctac ctcgaaacga atcgaaataa agaagcattg cgtgacctgg
    28381 cgaagacaaa cgaactggac aggacagcat actggccggc ggcttcggga atcgcgcata
    28441 tactgatgat caagggattg aaggactcga ctaacgacct ccagggaata ctcgcacttc
    28501 tgacggaatc gatttcgacc gtgacacatt cgaaatacta tcttgatgtc ctcaaaccta
    28561 tgtctgaagc ggttaatgaa atgcgcgaga aaaatgcttc cgcggatcag gcgaaagaga
    28621 tcctcgcgag aagatcgata gaactgccgg atgaaaaaac cagcatgatc ctaacggaca
    28681 acataaattt tgtattcgcg ttatacgccg aacgtgcgga cacctatgcg aaattgggtt
    28741 atcatggaaa agcgaaaatt gacctgaaga cggcatgccg aattatatgg aaggaagagt
    28801 tttgtgaatc taaatcgcct gcttccgtca ctgactagaa tacttattcc ttttttgttt
    28861 atgcctgaac ctgatcaaat atggtgttta tggattacag aaatttatcc atcctatcat
    28921 gttactgtga agataatttc tggtggtggt ttttgataga gagtat
//
LOCUS       DAOOLJ010000020        28224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_1475_length_28224_cov_19.139373, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000020 DAOOLJ010000000
VERSION     DAOOLJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 28224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28224
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1475_length_28224_cov_19.139373"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..676
                     /locus_tag="PLW16_05325"
     CDS             203..676
                     /locus_tag="PLW16_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPB70362.1"
                     /translation="MVRGDPESPSEGRPKDRLEEQPRGRPKDRPEGRLKDRRRIDTGK
                     GGETIAAAYLERMGYVVLERNYRCVFGEIDIVARDGAAICFVEVKSRRSARYGDPQLA
                     VTRRKQEKMIRTARHYLAGRGLEDRPARFDVAAIRLSPDGAGTVELIRNAFELKP"
     gene            complement(830..3112)
                     /locus_tag="PLW16_05330"
     CDS             complement(830..3112)
                     /locus_tag="PLW16_05330"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPB70363.1"
                     /translation="MQLEMKDVNPQDYLRVVLKHRWTIATVFAVVFVSVLIFTFTATP
                     IYMGTARVIIEKENPKVVSMQEVMTVDSSGLDYYQTQYKIIESRTVAREVVRRLKLDR
                     HEEFVPKPKEGLFYDIVYFVPNTIGYIRSLFKTDEQQEKSDSAIVDAFISRIKVTPIR
                     NSRLVDIGFEAKSPYMSAKIANTLAKAYIDNNLETKLRATQDAVAWLEGQIVTERKKV
                     EEAEQALLRYKEQKGIVTDFSTDVETITAQKLAEINKQVVEATSRRVEAETRYRQAAD
                     IRHSPEKLDSVPDVIRDELIRDIKKQEVEIQKRMSELSKKYGARHPQILALQEELNSL
                     QSRKANEIGRIVDSLYNEYKVALAKEHSLKNALNQQKGESLAMNVKAIDYNVLKRQAE
                     SSREMYDILVKRFKETSITEDIKTGNIRIVDLAEIPKSPVKPKKAQNLLLGLIVGLAL
                     GVGLAFFLEYLDNTIKVPDDIKNILKLPYLGPVPAIAAAEGGAVNGERNPADDLISHL
                     SPKSTASEAYRGIRTSILFSSAEQAPQVLIISSSGPREGKTITSANIAITMAQAGNRV
                     VILDCDMRRPRVHKIFSLSRDRGMSNLLVGNDELDNIVIHTDIPNLDVIPSGPIPPNP
                     SEVLSSQRMADLIEVLRGRYDRIIIDTPPITAVTDAVILSKVVDGVVLIVRAGVAHRE
                     LVRNAVEQLRQVNAHILGAVLNGVDMGRDSYYYYQYYYYYYGEDGERKKKTRSRKKMR
                     SRYYGADKEGRKKEADGGDA"
     gene            complement(3112..4170)
                     /locus_tag="PLW16_05335"
     CDS             complement(3112..4170)
                     /locus_tag="PLW16_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPB70364.1"
                     /translation="MATKARSAFRRLLPWGLVLLLVACAGAGDVVKVTQVSDDPLKGL
                     KQEDRAELEKLKKSLSTGKTGDELSDLLQKTPSFTVSEYLARHPDVRSQLDYKVGGYD
                     ILNILVYEEKDLSREGIPVSADGFITFPLIDRVRVADLTPSEIEKLIATKLAEGQFLH
                     NAHVSVIVTKYESRKYSVLGAVKNPGQLSLQAHDRVLDGISRAGGIEVAEGEKQEAMI
                     IRTQNPGTPQEQKIVVTIDLPGLLKGRDQISNILLADLDVLYIPKADYFYIMGEVKSP
                     GQYAFSKKDMTIVEAISMAGGFTPIAASGKTRIVRIEGGAEKIYQVNVDAITKGGKMI
                     QSVPIKPNDLIVVPESFF"
     gene            complement(4300..5775)
                     /locus_tag="PLW16_05340"
     CDS             complement(4300..5775)
                     /locus_tag="PLW16_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70365.1"
                     /translation="MKTRSNIMKGALLSGLIGLFLLCPLSGAYASIARVTAFTGDVVI
                     LSGANTIKLSKLGQAVNTGDKIQTKQGTAQLAFTDGAVLKINPHTTTMVQERMEERGA
                     WMFKSKKMARRITVFVGKLWFKSGVSDRSNYLQSPTAVCGLRGSDGDFGYDNLNTYIN
                     MYSGEASITGRVLQQFFQDPGVTAAQRNEVYQKLSQAYEQTRSAEATGQALNINEARL
                     ASLDVVKTAATVLSQNPDANVKNDAALATNVANANIAATAAAVNLEKNKNVASEAQAA
                     AQTAPPELSAMAQAAAKAAADELAAAQKQVEAAAAAAAKVNQAANDATKTSAALLAQT
                     SKSSSEAQAASSAANAAISAAKAQRDKAAATGLSEEAFKDADRAAAAAARAAEQAAAA
                     AARAEAAATAAAAAAAAEQAARDAARAEAEAAAAEEAAEDLGVTTTIAAPEPTTVEPT
                     TEEPATEEPAAEGPTTIPTTIQTTTSTSTTSTVEAASPSGT"
     gene            complement(5793..7046)
                     /locus_tag="PLW16_05345"
     CDS             complement(5793..7046)
                     /locus_tag="PLW16_05345"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPB70366.1"
                     /translation="MKKYLLSVVVSAFVFLILSPSALQAEGRIRFGNLTIVPSITAQT
                     IYDDNIYLGNGKSTGGLAEDDVRDWITHVKPGILFNYNFPERGNLALGYQGDWAFYAD
                     RSENDWNSQQGKLDFNYNAPAGLILGVTDTFIRSEDPYGDANQYALGQVTKRWTNDLR
                     TKAGWEFGKTFRTILFFNHYKQDYKYRKDDPQDYEDMEGGIGMGAKFLPKTWGFVRYF
                     YGQREYTEDNATLKLNDDTNSDFSWHRVSAGLTWDPAAKLQGELNVGYQWNSFDNDRT
                     PTGALRDDRDTWVAETSLNYKPIESTTLSLTLARAVRVTGADTNEYFDDTAVGFNLRQ
                     EFLRKFVFSAGVSYSLNEYENAFADNGKDDREDDNFTATAGLDYNIQDWLSVGVGYKY
                     QMKNSNADANDFTDNQVMATLKLSY"
     gene            7304..8011
                     /locus_tag="PLW16_05350"
     CDS             7304..8011
                     /locus_tag="PLW16_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB70367.1"
                     /translation="MKNPQGKAVSPIPSLTVVIPTLNEAATLPEVIAGVRPHTDDILV
                     VDGRSPDGTADVARSLGARVVYDHGRGKGEAIRTVIGHLERDIVVFIDADGSHDPADI
                     PALVAPILAGEADHVSGSRLVGGSSELHGGFDECFRLMGSSFITACINHRFGVVLSES
                     QNGFRAIRTEVLRDLGLGEDITTIEQEMIIKTLKKGYRMGEVPTHEHRRKAGYSKITV
                     RKVAFRYVYSMVKYLYF"
     gene            8202..9716
                     /locus_tag="PLW16_05355"
     CDS             8202..9716
                     /locus_tag="PLW16_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70368.1"
                     /translation="MRVLLFNPPGPDDRAFIREGRCTQEAGAWAVLWPPVTLTTAAAL
                     LRADGHHVRVVDFPAAGLGKADLAQLLLAEPPDLAVWSTGTPTLSFDLGLGASVREAA
                     PDAFTAVLGTHVSVRSEAALAHAGIDAVLRGEPEGVLRDLCRALERRRACASMQGRPS
                     PVSGDDADSASRGENEPDAPPDALASVRGLSWRGGHGSDIRHNPEADPLPAEAIPAPA
                     WDGLDLTPYRLPLKGRPFLIVAPVRGCPYVCSFCTAPLYYGSKLRRRPVEAVVAEIGD
                     NVARFGIHEFFLWADTFTADRRYVRAFCEAVITHRLDITWTCNSRVDTVDRETFTLMK
                     QAGLWMVSFGIESGSDRVLAACGKNTTAVQAATAVTTAHDLGIRTAGHFIFGLPGETE
                     ESLAETLALALALPLDVAQFYAAAPFPGTRLYDEAVRNGWLRSDAGTGSSFSQSAAVL
                     ELPDLPAARVDAFRREAYRRFYLRPRAIRRTLSMLEPRAAFQAVIGAQRFLRWI"
     gene            10040..10696
                     /locus_tag="PLW16_05360"
     CDS             10040..10696
                     /locus_tag="PLW16_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70369.1"
                     /translation="MNRALLWVALALLLGGCAMNVRTTGFLDPAAGTSAPPAGASFAV
                     LENAEVPNPIFDREIRGKIEILLTKHGYKIAAQDRADYLLTFSYDMSAGLRIGTMTSY
                     GPPRTQIVAVSDGRGGRTFRAITVPGAVTTRPTVTETFTRRLTLKVVDASPLRAGGPE
                     RVVWIGDTLSTETSSDLRYDIDYLLAAAFAWFGRDTGRQITVPIHGDNPDVGALRGEK
                     "
     gene            10729..12585
                     /locus_tag="PLW16_05365"
     CDS             10729..12585
                     /locus_tag="PLW16_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HPB70370.1"
                     /translation="MQKRTVLGIWDGHDAGAALVTGDRLLFAINEERLTRRKLEVGFP
                     ACAIQACLDFAGLNFARVDLVAASTTDPAKTLTRLVPRLKEEYYLIRRRKLAPRGADP
                     FKKAFKYRFTELAPNAASRALSRAWLKRRLGPLGLGAAPVAWVDHHFAHAAAAACCSG
                     FAEALVVTLDGVGDGRCGSLWRFADGRLALIRALPARASFGIFFEHVTNLMNMRELED
                     EGKVMALANYAYPIPDEENPLFAVIGADGLDVVSPHTSTAMFRELKKILWRYPSEQFA
                     YMAQRVLERGAAALVAEALRRTGLKKIAAAGGVFSNIKLNMRLAGLPGVENVYIFPHM
                     GDGGLAIGAAMAANLERNGVSAYALPHLYLGPDFSPADIAAAVDSRLRRKDKGGQGND
                     RREASPEGMQPGGGVSPLSGGQEAAAVSWVLADGLRWRRSADAPAEAARLILAGEIVL
                     WFRGRMEIGPRALGNRGILARPDSRDIKDRLNVVLKKRVWYQPFCPSMLAEDAPALIE
                     ATPAAIADNPFMTMAFRVRPERLPLMAGVINIDGTCRPQFVGDENPAYRDLLRHVRAA
                     LGYGVVLNTSFNIHGEPLVCSPDEAVRMLVDSGIRYLFLDDWLMENLHAKMD"
     gene            12569..14611
                     /locus_tag="PLW16_05370"
     CDS             12569..14611
                     /locus_tag="PLW16_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70371.1"
                     /translation="MRKWTETRAVPAAVLILYAALIGAFLYVLDYAAPPRIETALVKT
                     MPWVLRANMLLLVAGALWCWPDVAAFRREIFPRRPSPPSPPSSRPSRRPNARASALAL
                     LLVAAAVLVIFAAPRVHRIYYDEDIYANIGQNIALTHQAAMCNYGTFEYGEYFPHWLL
                     MNKEPTGWPFLMSLVFQGFGVDERYAFGLNNALYLGGILLAFFIAYRLARGGARAPSS
                     SPSQTARQAIGEGVGSRPYGNDLDIPFFAAFFAALAYAIIPHNLLWSNTAAVEPSAAF
                     FTGLVVLLLIVYLQTGRMRHLFVLACAIPLACQMRAESALILFWLVAAAFLLKPGPAA
                     GAEGAGDAEGGAPASAWERRLGVFARREVWIAGLLCGVLLVPHALHLFVMKGQSWGAE
                     GAKFAGSFFRPNLAVNGLYYLVNAKFPAILTLLALAGLACGSAPWRARLATLLWFLPF
                     WGIFLFFYAGSYGYGADVRFALVSFMPLAVLAGLGAGGILGVAGRLFPKDLDSRRSHA
                     PDGPAGGGNDGGTVGRGEAQCAARDAGGRRRFGRTGVAALLVLVILFCWLPFLPLIRT
                     VGQEAWAARYDHLYAGRFAKMIPERSIVLTHNPTMFLLWGQGAIQAGAGLDNPDVVAF
                     LMKKYPGHVYFHENYWCTVPGANQEMCRAIRAKYDLREVAAFTERNHRYALYQIKR"
     gene            complement(14710..15537)
                     /locus_tag="PLW16_05375"
     CDS             complement(14710..15537)
                     /locus_tag="PLW16_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70372.1"
                     /translation="MKFARRIAILVAAIALLAAPATAEIRTIVTATASILSTGETTVS
                     YDILNAGNEEVLHVRVATFLGLIADRSDPLGDNAPGKTVRCTRTLDTRALVPGHYILA
                     ARVNFEDRAGATHAVHHFIPLAFGAAAPGVQTASPLTVAVSDAEFNAKSPLNTKDDIT
                     LILKNTGGAPLEPVVSFFLPDGVTTAEPEKAYPVAAGETKTVKLPLNFAPTVARDLPY
                     RAVVWYEAQGRHDARLLEGTIRVTQKPVLFKAFLIGAGAVLVIVVASVVVIRRRKQA"
     gene            complement(15582..17057)
                     /locus_tag="PLW16_05380"
     CDS             complement(15582..17057)
                     /locus_tag="PLW16_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70373.1"
                     /translation="MNLRTQARRLWIICIVVVALGTPGAARTEEVCIRPPAYPGATAP
                     DDGVWTTPLPACEAGRTYHFSGEFRRDTWDDRTYPWISLWGQKFHLNAHCRTGAPQTF
                     RIRVTCPERIADPTFRFGAQPASAGGVPQRFCLGTHGLAAAPTEMAPNAALWTPPPSI
                     AGFFPIGVYGADPSRLAEVRAIAFNTVILGGEGEPLAKAIASARNERLRYVLSTPGDP
                     EHLKVYLDRLDAVPRVRGGGLLGFYVADEPELRSVPATRAEDVRRLLADRFPGVATAM
                     AIVRPQHARDYLGAADYFMMDQYPFPHRPMTWLSDAMDEAAADTGRDRLMSVIQAFAK
                     GEAWPSLPGWRQMDCLAFLSIVHGSRGIFFYTWSVMGRTEEGRARLGRVVGRLNRVYP
                     WLLVKNAERAVDVEMRSENRVDPRGRPAVHACVKEREEGLLLIAVNTIGANVSARLKI
                     GAVGETVEIFSGRVHPVVDGAILADLDAHETKAFLIKSGRE"
     gene            complement(17054..19408)
                     /locus_tag="PLW16_05385"
     CDS             complement(17054..19408)
                     /locus_tag="PLW16_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB70374.1"
                     /translation="MERTAFGIYVFVLVASIVFFGAVHTWAYATVFLLILAASLLLIA
                     RDWIPAFKRNRAVPLTITGAEALFVLMVLLLVVQMTPLPGWLLTKLSAEAKAAGLSAL
                     PATASVADPSLKNAWFTLAPYAYPVRMSLIRWTAYGLLFFGLCRTLNSRRRIEFLVVA
                     LLATAVANALYGIAQTYSGSPYILWWKKFSQGAKDVSGTYINRNHFAGFMEMGIALAI
                     AYAGAIVAGGRRRIVHARGAWTFKDRFILLFSVESKALRQILVIFSGVIMGVALILSA
                     SRAGIVSALAGLFVMGVFFLARRDHRAKGGIVLALFGVTVLYAAFAGMDYTIERFQKL
                     DFDQAGRPRYVERAVLLHDDYPLVGVGVGNFRHAYPKYQAPEDKGRFIDFLHSDWVQF
                     FTEAGYTGMLALIAGLGWFVYRLVRTWSIRGDPFAVCLGLAPVGALSSIGTHSLFDFN
                     LHIPANFMMLAAVCAIGLSALHLERQRREDVMRWRRIHLRMAGAGGIAAAFVIVLIVW
                     TGLWTVRHAVAEAHCATVGNLTMNLEEHQSLDNIRSAIAWDGANAEYRSKLARELMDR
                     RDRLMQAAYAVREAATSETTGPRATPAKPAAGAVGHEGATAPDAAPAKTRPAAPSLTT
                     AQAAWRASHGPVIAALEDAIRRNPLNAETHVRLAWEYTYLWDRHDYMTRWMRGADASM
                     ERAAYYAGTGSINPHLHVDMGNYWAVRSKNTGPSSPPPELSWLKASWHYGQALSLTGR
                     KTKDGMKPDRNFVAEITNFVRNLYPDDPARLAEALGGEAGGARE"
     gene            complement(19408..21225)
                     /locus_tag="PLW16_05390"
     CDS             complement(19408..21225)
                     /locus_tag="PLW16_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HPB70375.1"
                     /translation="MPAKRTKQSLNPKTRNERVSQSPNGEVFPLPKPEDYVKEFRRLK
                     RLVARHRRAGREIVVVMGVGFVGAVMAGIVADAVRKPVVQHADPKAPRRGTASGGRRP
                     PKTTQKIDPALTRPGQSAHAPVPGEPAKFVIGMQRPSPRSYWKIPYLNRGIAPVEAED
                     PEVAPLIARCVLEKKTLTATYTYDALSLADCVVVDVQCDYFKETLGNCRQGRAEIAAL
                     EESLRVIGERISPDCLVLIETTVPPGTTEYVAYPILKKAFERRGLAAEPLLAHSYERV
                     MPGREYVASIRDFWRVCSGVNAASRTRVATFLSEVLNVETFPLTVLDRPIESETCKIV
                     ENSYRATILAFLDEWSLFAERNGVDLMKVIQAIKVRPTHSNMIFPGPGIGGYCLPKDG
                     GLGVWSYRHLMGFEDDIFKITPMAIDINDTRALHAAELVRDALRNMGKIVAAAKVVIL
                     GASYREDVGDTRYSGSEALVRKLAEMGAEIGVHDPYVKHWWELEKQDTYPAPGHSWAR
                     FFRNQESLTDLRIEPDLSAALKGADAVVLAVRHAAYRTLDPAKIVEMAGRPVAVIDCF
                     GLLDDAAIRRYFELGCEVKGLGRGHVKRIKDQTSSGRAD"
     gene            complement(21361..22800)
                     /locus_tag="PLW16_05395"
     CDS             complement(21361..22800)
                     /locus_tag="PLW16_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPB70376.1"
                     /translation="MGEKTDFNDLTARKILCIGAGYVGGPTMAVIAHQCPDCRVTVVD
                     VNAERIAAWNSETLPVYEPGLDAVVRAARGRNLFFSTDIAGGIRANDIIFVSVNTPTK
                     TFGAGAGMAADLQYWEKTAREILEYAESPKIVIEKSTLPVKTALAMERILNSRGGDVR
                     FDVLSNPEFLAEGTAIRDLENPDRVLIGSRETGTGLAARHVLEEIYARWVPRERILTS
                     NIWSAELSKLVSNAFLAQRISSINAVSALCEKTEANVTEVARAVGMDSRIGPKFLNAS
                     VGFGGSCFKKDILNLVYLCRAYGLDDVAAYWEGVVAINEYQKERFVLNMMAAMFNSLA
                     GKRIALYGFAFKADTGDTRESPAIFVAERLLAERAEVVITDPQALSNARIWAQGLTDR
                     GTGSLFETGAGPKIAFATDPVAAAAGCHALTVMTEWPLYRGLDYERIFRTMVQPAFVF
                     DGRNILDHERLHAIGFNVYPIGRPPLTHF"
     gene            complement(22841..23797)
                     /locus_tag="PLW16_05400"
     CDS             complement(22841..23797)
                     /locus_tag="PLW16_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70377.1"
                     /translation="MTARILVTGGAGFLGSHLSDRLLAQGHDVLCLDNLFTGSKDNIR
                     HLLGHPRFEFIRHDIINPIYLEVDEIYNLACPASPVHYQVNPIKTIKTSVMGAINTLG
                     LAKRVHAKILQASTSEVYGDPETHPQPEAYWGRVNPIGIRSCYDEGKRAAECLMMDYH
                     RQNGVAIKIVRIFNTYGPRMAPDDGRVVSNFIVQALRGEDLTLYGDGTQTRSFCYVDD
                     LIDGLIRMMDSPPDFIGPVNMGNPTEFTIRELAEAVLRLTGAPGKIVHRPLPQDDPTH
                     RQPDITLARSRLNWEPSIPLEAGLRQTIRYFESINDKRGPGL"
     gene            complement(23928..24719)
                     /locus_tag="PLW16_05405"
     CDS             complement(23928..24719)
                     /locus_tag="PLW16_05405"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70378.1"
                     /translation="MIDLHSHILPGLDDGARDLERSLDMARASVADGVTDMVCTPHWV
                     LGKYENTRAAILAACADLRDRLAESQIPLTLWPGAELHVDTGIPERIRRGELLTLIDG
                     GKYVLIELPEESLPSNLEDFFWALQLDGIKPILSHVERNPVLQRDPMRLFRWVEMGIL
                     AQITAASLVQEFSEEILDFSQELIEHRLVHILVSDAHGLKRRSPKLSAARDVVASIAG
                     EPAARRMTEEIPRRILEGKFVEPDDPLPYRSAKRGLRSYFDRFFK"
     gene            25109..25963
                     /gene="rsmI"
                     /locus_tag="PLW16_05410"
     CDS             25109..25963
                     /gene="rsmI"
                     /locus_tag="PLW16_05410"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB70379.1"
                     /translation="MNPQGNRENGCLFIVGTPIGNLEDITLRAIRVLKEVRLVAAEDT
                     RHSRKLLAAHGITTPLISLHEHNEAARAGLLLGKLQAGQDVAYISDAGTPAISDPGAR
                     LVRAALAAGVRVVPVPGASAVASALSVSGFSADAFLFAGFLPARAAARRLVLAALKGE
                     PRTLVFYESPRRLTATLKDLAAVFGDREAVLCRELTKVFEEVLRGPLSALAAKLETGA
                     VRGEVTLVVAGSGGVNTETSVDAADLREMAARLAREGLSRRDRVEIIARETGLPRRLV
                     YQKIGLET"
     gene            26114..26506
                     /locus_tag="PLW16_05415"
     CDS             26114..26506
                     /locus_tag="PLW16_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70380.1"
                     /translation="MGENDGGFPGAMADGGTPVGRRPGPGEVIYDLTERVEGRVGPAA
                     AEIDRAGGRRIFDLTEVIEEGPVFPPEAEAFREEMIRRVETAAGAIARQILPAVAERI
                     AREGFPEIAARIVREEIEKLKSETEDAP"
     gene            26503..27225
                     /locus_tag="PLW16_05420"
     CDS             26503..27225
                     /locus_tag="PLW16_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HPB70381.1"
                     /translation="MSGRLAEQGGRALRHRRDDAPPTVHVAVPMDLRHRRTEGAAALA
                     RVAVRLLAVALAVLLLGACAGKIPHTLKPEYKDAGIRLIAVLPVQGENMDPRLARLLR
                     EKVLMGLYFKGYPKIPLTLVDEKLATFYQAQTGAQGVVAPRVAGELLGIDAVLYISLV
                     ECRTAYRALYAQTTVAARLELRSARTGETLWRVQDREAARNFGFTSEELEMKSSEVCE
                     AAMQTVVDRALYALPDGPDRLG"
     gene            27316..28149
                     /locus_tag="PLW16_05425"
     CDS             27316..28149
                     /locus_tag="PLW16_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB70382.1"
                     /translation="MKKGYWIGGGVLAICLVFWVTTVTAQGIRTMQVGKGGARVTAID
                     GSAQVRIGGQGAPRALRVGEHLAGADEVATGSRSRIEMALPDGSRIRFSENTRFTLLE
                     VEPGSYRRVRSIRVNVIVGRVWAKVARAIGARGRFEVIAENSVAGVRGTVYRVDVNED
                     KSNLIRVYDGTVHVSGSAEAPTKPPAPAVIGPPTKVEGPKSIPGPRVVSMEEWVYILK
                     SMQQIVVRPDGSADEPKDFAPEEDRDPWVDWNRARDQEADREGGADAAPQPRGGVPCE
                     N"
BASE COUNT         4431 a         8983 c         9711 g         5099 t
ORIGIN      
        1 aacaaaccgt gcttccgtgg tgaggcacga cagactgttt tgtgatttcg acggtgcctt
       61 tttcatttcg accgaaggga gaaatcttta agatttctcg tcgctggcgc tccttgaaat
      121 gacatgatgc aatcattcta caggaaatgg tcaacaccaa aggcatcgtg gtagctttgc
      181 agcaaagggg gaagggggcg gaatggtcag gggtgatccg gaaagcccgt cggagggtcg
      241 gccgaaggac cggctggagg aacagccaag gggtcgtcca aaggaccggc cggagggaag
      301 gctgaaggac cggcggcgca tcgatacggg gaaggggggc gagacgatcg ccgcggccta
      361 cctcgagcgg atgggctatg tcgtcctgga gcggaactac cgctgtgtct tcggagagat
      421 cgacatcgtc gcccgagacg gggccgcgat ctgtttcgtc gaggtgaaaa gccgccgttc
      481 ggcgcgctac ggagacccgc aactggccgt cacccgaagg aaacaggaaa agatgatccg
      541 gacggcccgg cactacctgg ccggccgcgg cctggaggac cggcccgccc gcttcgacgt
      601 cgcggccatc cgcctgtcgc cggacggcgc cggcacggta gagctgatcc ggaacgcctt
      661 cgaactcaag ccctgaaaga aaacggcgcc tgatggctca ggcgccgtcg ttgtccccgg
      721 ataattcccc acgccgccgg gcgcacagcc ggggcaggtc tttaaaatcc gcttttgatc
      781 acacccccat cgtcgccccg gaatcggggt ggcggcgctt acccgcggct caggcgtcgc
      841 cgccgtccgc ctcctttttc cggccttcct tgtccgcgcc gtagtagcgg cttcgcatct
      901 tcttgcgcga ccgcgtcttc ttcttccgct cgccgtcctc gccgtagtag taatagtagt
      961 actgataata gtagtagctg tcgcggccca tgtccacgcc gttcaggacc gcgcccagga
     1021 tgtgggcgtt gacctgccgg agctgttcga cggcgttccg gacgagttcg cggtgggcca
     1081 cgcccgccct cacgatcagg acgacgccgt cgacaacctt ggagaggatg acggcatccg
     1141 tcaccgccgt gatgggcggc gtatcgatga tgatccggtc atagcgcccg cgcaggacct
     1201 cgatgagatc cgccatccgc tgcgaactca ggacctccga cggattgggc ggaatcggcc
     1261 cggaggggat aacgtcaaga ttggggatgt ccgtgtggat gacgatgttg tccagctcgt
     1321 cgttgccgac cagcaggttc gacatcccgc ggtcccttga cagggagaag atcttgtgga
     1381 cgcgcggccg acgcatgtcg cagtcgagga taacgacgcg gttgccggcc tgggccatgg
     1441 tgatcgcgat gttggcggag gtgatcgtct tcccttcgcg cggccccgag ctcgaaatga
     1501 tcaggacctg cggcgcctgc tccgcggacg agaagaggat gctcgttcgg atgccccgat
     1561 aggcctcgct ggccgtcgat ttgggcgaga ggtgcgagat gaggtcgtcc gccggatttc
     1621 tctcgccgtt gacggcgccg ccctcggcgg cggcaatggc gggaacgggc cccagatacg
     1681 gcagcttcag gatgttcttg atgtcgtcgg ggaccttgat cgtgttgtcc aggtattcga
     1741 ggaagaacgc gagccccacg ccgagggcga gccccacgat cagccccagg agcaggttct
     1801 gggccttctt gggcttgacg ggcgacttgg ggatctccgc cagatccacg atacggatgt
     1861 tcccggtctt gatgtcctcg gtgatggagg tctccttgaa gcgcttgacg aggatgtcgt
     1921 acatctcccg gctgctttcc gcctggcgct tcagcacgtt gtagtcgatc gccttcacgt
     1981 tcatggcgag gctctcgccc ttctgctggt tcagggcgtt cttcagggaa tgctccttgg
     2041 ccagggcgac cttgtattcg ttgtagaggg agtcgacgat ccggccgatc tcgttggcct
     2101 tgcgcgattg cagggagttg agctcctcct ggagggccag gatctggggg tggcgcgcgc
     2161 cgtacttctt cgatagctcg gacatccgtt tctggatctc cacctcctgc ttcttgatgt
     2221 cgcggatgag ctcgtcccgg atgacgtccg gcacggagtc gagcttctcc gggctgtggc
     2281 ggatgtccgc cgcctgtcgg tagcgggttt ccgcctccac gcggcgggag gtggcctcga
     2341 cgacctgctt gttgatctcg gcgagcttct gggcggtgat cgtctccacg tccgtggaga
     2401 agtccgtcac gatccccttc tgctccttgt agcgcaggag ggcctgttcc gcctcctcca
     2461 cctttttccg ttcggtgacg atctggccct cgagccaggc cacggcgtcc tgcgtggccc
     2521 gcagcttggt ctccaggttg ttgtcgatat aggccttggc cagggtattg gcgatcttcg
     2581 ccgacatgta cgggcttttg gcctcgaagc cgatatccac caggcggctg ttgcggatcg
     2641 gcgtgacctt gatccggctg atgaaggcat ccacgatggc ggagtcggat ttctcctgct
     2701 gctcatccgt cttgaagagc gaacgaatat agccgatcgt gttcgggacg aagtacacga
     2761 tgtcgtagaa caggccttcc ttcggtttcg ggacgaactc ctcgtgccgg tcgagcttga
     2821 gccgccgcac gacctcccgg gcgacggtgc gggactcgat gatcttgtac tgggtctggt
     2881 agtagtccag gcccgaggag tccaccgtca tgacctcctg catggagacg accttggggt
     2941 tttccttttc gatgatgacc cgggccgttc ccatgtagat gggcgtggcg gtgaaggtga
     3001 agatcagcac ggagacgaag acgacggcga acaccgtcgc gatcgtccag cggtgcttca
     3061 gcacgacgcg caggtagtcc tgcgggttga cgtctttcat ttcaagctgc atcagaagaa
     3121 gctctccggt acgacaatca ggtcgttggg tttgatgggg acggactgga tcatcttgcc
     3181 gcccttcgtg atggcgtcga cgttcacctg gtagatcttc tcggcgccgc cctcgatccg
     3241 gacgatccgg gtcttgccgc tcgcggcgat cggcgtgaag ccgcccgcca tgctgatcgc
     3301 ctcgacgatc gtcatgtcct tcttgctgaa ggcgtactgg ccgggggact tgacctcgcc
     3361 catgatgtag aagtagtccg ccttcgggat gtagagcacg tcgagatcgg cgaggaggat
     3421 gttcgagatc tggtcccgtc ctttaagaag gccgggcagg tcgatcgtga cgacgatctt
     3481 ctgctcctga ggcgtgccgg ggttctgggt ccggatgatc atcgcctcct gcttttcgcc
     3541 ttccgcgacc tcgatgccgc cggcgcgcga gatcccgtcg agcacgcggt catgggcctg
     3601 gaggctgagt tgtcccgggt tctttacggc tcccaggacg gagtacttgc ggctctcgta
     3661 cttggtgacg atgaccgaga catgcgcgtt gtggagaaac tggccttcgg cgagcttggt
     3721 cgcgatgagt ttctcgatct ccgacggggt caggtccgcc acgcgaacgc ggtcgatcag
     3781 ggggaaggtg atgaagccgt cggcggagac cgggatgcct tctcgggaaa gatccttctc
     3841 ctcatagacg aggatgttga ggatgtcgta cccccccacc ttgtagtcca gctggctgcg
     3901 cacgtcggga tgtcgggcga ggtactccga gacggtgaag ctgggcgtct tctgcagaag
     3961 atcgctcagt tcgtctcccg tcttgccggt cgaaaggctt ttcttcagtt tctccagttc
     4021 ggcgcggtct tcctgtttga gccccttgag ggggtcgtcg ctgacctggg tcaccttgac
     4081 gacgtcgccc gcccccgcgc aggcgacgag gaggagaacc agtccccacg ggagcaggcg
     4141 ccggaaggcg cttctcgctt ttgttgccat cgttctcgtt tcgtccgcct cataaaaaag
     4201 ttgcagggcg tcactggggc ccgccctgca aatgggttca acgttcagac gtcaatgccc
     4261 acgttcgccg tggatcaaag gttccattcg gcccgtgaat caggtccccg acgggctcgc
     4321 cgcctcgacg gtcgacgtcg tcgacgtgga ggtcgtggtc tggatcgtcg tcgggatggt
     4381 tgtcggtccc tccgcggccg gttcctccgt tgccggctcc tctgtagtcg gttccaccgt
     4441 tgtcggctcc ggagcggcga tcgtcgtcgt gacgcccaga tcttccgcgg cctcctccgc
     4501 ggccgccgct tctgcttccg cgcgggccgc atcccgggcg gcctgttcgg ccgccgccgc
     4561 cgccgccgcc gccgtcgccg cggcctcagc tctggctgcc gcggccgctg cctgttctgc
     4621 agccctggcc gccgccgccg ccgccctatc cgcgtcctta aaggcttcct ccgacaagcc
     4681 cgtggccgct gccttgtccc tttgcgcctt ggcggcgctg atggccgcgt tggccgccga
     4741 tgacgctgcc tgcgcttcgc tggacgattt cgacgtctgg gcaaggaggg ctgcactggt
     4801 cttcgtggcg tcgttggcgg cctggttgac cttggcggct gccgcggccg ccgcctcgac
     4861 ctgtttctgt gccgccgcca actcgtctgc cgcggctttc gccgccgctt gcgccattgc
     4921 cgacagttca ggtggtgccg tctgtgccgc ggcctgtgct tccgacgcga cgtttttgtt
     4981 cttctccagg tttaccgcgg cggcagttgc cgcgatgttg gcgttcgcga cgttcgtggc
     5041 cagggccgcg tcgtttttca cgttggcgtc ggggttctgc gaaagcacgg ttgccgccgt
     5101 cttcacgacg tccaacgatg ccaggcgggc ctcgttgatg ttgagggcct gtcccgtggc
     5161 ctcggcggag cgcgtctgct cataggcctg ggacagcttc tggtagactt cgtttcgctg
     5221 agccgcggtg acgccggggt cttggaagaa ctgctgcagg acgcggccgg tgatggacgc
     5281 ctcgccgctg tacatgttga tgtaggtgtt caggttgtcg tagccgaaat ccccgtcgga
     5341 gccgcgcaga ccgcagacgg ccgtcgggct ctgcaggtag ttgctccggt cggagacgcc
     5401 ggacttgaac cagagcttgc cgacgaagac ggtgatgcgg cgcgccatct tcttcgactt
     5461 gaacatccag gcgcctctct cctccatgcg ctcctggacc atcgtggttg tatgggggtt
     5521 gatcttgagc acggcgccgt cggtgaaggc gagctgtgcc gtgccctgtt tcgtctggat
     5581 cttgtcgccg gtgttgaccg cctgtcccag cttcgacaat ttgatcgtgt tcgccccgga
     5641 caggatcacc acgtccccgg tgaacgccgt cacccgggcg atgctggcat aggccccgct
     5701 cagaggacag agaaggaaga gcccgatcag tcccgagagc agcgcccctt tcatgatgtt
     5761 cgatcttgtc ttcatcgttt aagtcctcct catcaatagc tcagtttaag cgtggccata
     5821 acctggttgt cggtaaaatc gttggcgtcc gcattggagt tcttcatctg gtacttgtag
     5881 ccgacaccga ccgacagcca gtcctggatg ttgtagtcga gccccgccgt ggcggtgaag
     5941 ttgtcgtcct cgcggtcgtc cttgccgttg tcggcaaagg cgttctcgta ttcgttcagg
     6001 ctgtagctga cgcccgcgct gaagacgaac ttccgcagga attcctggcg caggttgaac
     6061 ccgacggcgg tatcgtcgaa atactcgttc gtgtccgccc cggtgacgcg cacggcgcgc
     6121 gcgagcgtga gcgacagggt ggtcgactcg ataggcttgt agttgaggga ggtctcggcg
     6181 acccaggtgt cgcggtcgtc gcggagcgcc cccgttggtg tgcggtcatt gtcgaaggag
     6241 ttccactgat agccgacgtt cagttcaccc tggagcttgg cggcggggtc ccaggtcaga
     6301 ccggccgaca cccggtgcca ggaaaaatcg gagttggtgt cgtcgttgag cttcagggtc
     6361 gcgttgtcct cggtgtattc ccgctgtccg tagaagtagc ggacgaatcc ccaggtcttg
     6421 gggaggaatt tcgcccccat gccgatcccg ccttccatgt cctcgtagtc ctgcgggtcg
     6481 tccttccgat acttgtagtc ctgcttgtag tggttgaaga aaaggatcgt gcggaaggtc
     6541 ttgccgaact cccagccggc cttcgtccgg aggtcgttcg tccagcgctt cgtgacctgg
     6601 cccagcgcgt actggttggc gtcgccgtag gggtcctctg accggatgaa cgtgtcggtc
     6661 acgccgagga tcaggcccgc cggggcgtta tagttgaaat cgagcttgcc ctgctggctg
     6721 ttccagtcgt tttcgctcct gtccgcgtag aacgcccagt cgccctggta gcccagggcg
     6781 aggttgcccc gctccgggaa attgtagttg aagaggattc cgggcttgac gtgcgtgatc
     6841 cagtccctca catcatcttc ggccaatcca cccgtgcttt tcccgtttcc caggtagatg
     6901 ttgtcgtcgt agatggtctg ggcggtgata ctgggaacga tggtcaggtt tccgaaccgg
     6961 atccgtccct ccgcctgcag ggccgacgga gacaggatca gaaaaacgaa tgcggatacc
     7021 acaacgctca ataggtactt cttcacggcg cacccccttc tgttcgatga tgaaaatggc
     7081 gaaaaggccg ccccgaagag cggtcccgta aacccgattg aattgacccg ttgattccag
     7141 tcaatagcca acgtacacgc gcgtgtcaag cagtttctgc cgtatcttgt gtgaagttcg
     7201 tcacacagga aatcgggaat cacgcgtacc ggatacgaat atcgatttac aaacgttgga
     7261 gggtacgcta taagggccga aactttttgt caaggatttt tttatgaaga accctcaggg
     7321 aaaagctgtt agtccgattc cgtccctgac cgtcgtcatt cccaccctca acgaggccgc
     7381 caccctgccc gaggtcatcg ccggcgtccg gccgcacacg gacgacatcc tcgtcgtcga
     7441 cggccgctcc ccggacggca cggccgacgt ggcccgttct ctgggcgccc gggtcgttta
     7501 cgatcacggc cgcgggaagg gcgaggcgat ccgcaccgtg atcggccacc tcgagaggga
     7561 tatcgtcgtc ttcatcgacg ccgacggctc ccacgatccg gccgacatcc ccgcgctcgt
     7621 cgcccccatc ctcgcgggag aggcggacca cgtctccggg tcccgcctgg tcggcggttc
     7681 gagcgagctg cacggcggct tcgacgagtg cttccgcctg atgggcagct ccttcatcac
     7741 ggcctgcatc aaccaccggt tcggcgtcgt cctgtccgag agccagaacg gcttccgggc
     7801 gatccggacg gaggtcctgc gcgacctggg cctcggggag gacatcacga cgatcgaaca
     7861 ggagatgatc atcaagacgc tgaagaaggg ctaccggatg ggcgaggtcc ccacgcacga
     7921 gcaccggcgc aaggcgggct attccaagat caccgtccgc aaggttgcct tccgctacgt
     7981 ctacagcatg gtgaaatacc tctacttcta aacaaaaaat tagggacgtt gccctgtttt
     8041 gattcgcccg ggccggcgcg cgagcgggga aaacgaatag agaaaaaatg gggtaagtcg
     8101 ggacgtcgcc ctatgttggg taacgccccc aaaaatgtgc gggagcggcc gaaaaacgcc
     8161 tgcttcacaa agcttattta ccggagggtc caggcttcag gatgcgcgtg ctgctgttca
     8221 acccgcccgg tccggacgac cgggccttca tccgcgaggg gcgctgcacc caggaggccg
     8281 gggcctgggc ggtcctgtgg ccgcccgtga ccctcaccac cgccgccgcc ctcctgcggg
     8341 cggacggcca ccacgtccgg gtcgtcgact ttcccgcggc cggtctcggt aaggccgacc
     8401 tcgcgcagct cctcctggcc gagccgcccg acctggcggt ttggagcacc ggcacgccca
     8461 ccctgtcgtt cgatctcggc ctcggggcct ctgtgcggga agcggcgccc gacgccttta
     8521 ccgccgttct ggggacccac gtgtccgtcc ggtccgaggc cgccctggcg catgccggga
     8581 tcgatgcggt gctgcgcggc gagccggagg gcgtcttgcg ggatctctgc cgggcgctcg
     8641 aacgccggag ggcgtgcgcg tcgatgcagg ggcgcccatc cccggtttcg ggcgacgacg
     8701 cggattccgc ttcccgcgga gagaacgaac cggacgcccc tccggacgcc ttggcctccg
     8761 tccgcggcct gtcctggcgc gggggccacg ggtcggatat ccgccacaac cccgaggccg
     8821 accccctgcc ggcggaggcc atccccgccc cggcctggga cggcctcgac ctcaccccct
     8881 accggctgcc gctcaagggc cgaccctttc tcatcgtggc cccggtccgg ggctgccctt
     8941 acgtatgcag tttctgcacg gccccgctgt actacgggtc gaagctccgc cgccgcccgg
     9001 tcgaggcggt cgtcgccgag atcggggaca acgtcgcccg cttcggcatc cacgaattct
     9061 tcctctgggc ggacaccttc acggcggacc gccgctacgt gcgcgccttc tgcgaggcgg
     9121 tgatcacgca ccgtctcgat atcacctgga cgtgcaacag ccgcgtggac acggtggacc
     9181 gcgagacctt caccctgatg aaacaggccg gcctctggat ggtcagcttc ggcatcgagt
     9241 cgggcagcga ccgggtgctg gcggcctgcg ggaagaacac gaccgccgtg caggcggcga
     9301 ccgccgtcac gacggcccac gacctgggga tccgcacggc ggggcatttc atcttcggcc
     9361 tgcccggcga gacggaggag agcctggcgg agacgctggc cctcgcgctg gcgctccccc
     9421 tcgacgtcgc gcagttctac gcggcggcgc cgtttcccgg cacgcgcctc tacgacgagg
     9481 ccgtccgcaa cggctggctg cggagcgacg cggggacggg ctcatccttt tcccagagcg
     9541 cggcggtcct cgagttgccg gacctgccgg ccgcgcgcgt cgacgccttc cggcgcgagg
     9601 cgtaccgccg tttctacctg cggccgcggg cgatccggcg gacgctctcg atgctggagc
     9661 cccgggcggc gttccaggcg gtgatcggcg cccaacgttt cctccgctgg atttgaagat
     9721 cccgggcgcc gggctgtcgg gcccgtcgcg gcggggaggc cggtcgaaaa ggtggccggg
     9781 gcgggcggtc ggacgccgaa tggctggatt tatccggcga ggtgggttaa aaagaagggg
     9841 aacgtccgcc ggcgcggaag gcaacacgca cccgcgacgg aaggaatcga tccgtgatga
     9901 gacatttgca tcgtttcatt tcgaggagcg tgagcgacga gaaatcttca tggtggccgt
     9961 ttctaaaaga cttctccctt cggtcgaaat gacaaaacgg ctgtaaaggt ctcgtgatac
    10021 gcatcgggga ggtgccgcga tgaacagagc ccttctttgg gtcgccctcg cgctcctgct
    10081 gggcggctgc gccatgaacg tccgcacgac cgggtttctg gacccggccg ccgggacgtc
    10141 cgcgccgccg gccggggcgt cctttgccgt cctggaaaac gccgaggtcc cgaaccccat
    10201 tttcgaccgg gaaatccgtg gaaagatcga aatcctgctc acgaagcacg gctacaagat
    10261 cgccgcccag gaccgggcgg attacctgct cacgttcagc tacgacatga gcgccgggtt
    10321 gcgcatcggg acgatgacct cctacggccc gccgcggacg cagatcgtcg ccgtgtcgga
    10381 cggccggggc ggacgaacct tccgcgccat cacggtgccg ggcgccgtca ccaccaggcc
    10441 caccgtgacg gaaaccttca cccggcgcct gacgctcaag gtcgtcgatg caagccccct
    10501 gagggcgggc gggccggagc gggtcgtctg gatcggggac acgctgagca ccgagacgtc
    10561 gtcggacctg cggtacgaca tcgactacct gctggccgcc gccttcgcct ggttcggccg
    10621 cgataccggg aggcagatca cggttcccat ccacggggac aaccccgatg tcggggccct
    10681 gcggggagaa aagtagggcg acgcccctat ttttcgtgag aggaggacat gcaaaagcgc
    10741 acggtgctgg ggatctggga tgggcatgac gcgggggcgg ccctcgttac gggcgaccgg
    10801 ctcctgttcg ccatcaacga ggagcgcctg acgcgccgta agctcgaggt cggcttcccc
    10861 gcgtgcgcca tacaggcctg cctcgacttt gccgggctga atttcgcccg agtggatctc
    10921 gtggccgcct cgacgacgga cccggccaag accctgacgc gcctcgtccc acgactcaag
    10981 gaggagtact acctcatccg gcggcgcaag ctggcgcccc gcggtgcgga ccccttcaag
    11041 aaggccttca agtaccgctt cacggagctg gccccgaacg ccgcctcgcg cgccctaagc
    11101 cgcgcctggc tgaagcgtcg gctgggaccg ctgggcctgg gcgccgcccc cgtcgcgtgg
    11161 gtcgatcacc actttgccca cgccgccgcc gcggcctgct gcagcggttt cgccgaggcc
    11221 ctggtcgtca ccctcgacgg cgtcggcgac gggcgctgcg gcagcctgtg gcggttcgcg
    11281 gacggccgcc tggcgctcat ccgggccctg cccgcccgcg cctcgttcgg catcttcttc
    11341 gagcacgtga caaacctcat gaacatgcgc gaactcgagg acgagggcaa ggtcatggcc
    11401 ctggccaact acgcctaccc gatcccggac gaggaaaacc cgctgttcgc cgtgatcggc
    11461 gcggacggcc tggacgtcgt ctcgccgcac acctcgacgg cgatgttccg ggagctcaag
    11521 aagatcctct ggcgctaccc ctcggagcag ttcgcctaca tggcccagcg cgtgctcgag
    11581 cgaggcgccg ccgccctcgt cgccgaggcc ctccgccgga ccggcctgaa gaagatcgcc
    11641 gcggcgggcg gcgtcttctc gaacatcaag ctcaacatgc gcctcgccgg gctccccggt
    11701 gtggagaacg tctacatctt cccccacatg ggcgacggcg gcctcgcgat cggcgcggcg
    11761 atggcggcga acctggagcg aaacggcgtt tccgcctacg ccctccctca tctctacctc
    11821 ggcccggact tttcgccggc cgacatcgcc gccgccgtgg attcccgcct ccgcaggaag
    11881 gacaaggggg ggcagggcaa cgacaggcga gaggcgtctc ccgaagggat gcaaccgggc
    11941 gggggcgttt ccccgttgtc cggcggccaa gaagcggccg ccgtttcctg ggtcctggcc
    12001 gacggcctgc gctggcggag aagtgcggac gcgccggccg aggcggcccg cctgatcctc
    12061 gccggcgaga tcgtcctgtg gttccgcggc cggatggaaa tcggcccgcg cgccctcgga
    12121 aaccgcggca tcctggcccg gccggactcc cgcgacatca aggaccggct gaacgtcgtc
    12181 ctgaagaagc gcgtctggta ccagcccttc tgtccgagca tgctggcgga ggacgccccg
    12241 gcgctcatcg aggcgacccc cgccgcgatt gccgacaacc ccttcatgac gatggccttc
    12301 cgcgtcagac ccgagcgcct gcccctcatg gcgggcgtga tcaacatcga cggcacctgc
    12361 cgcccccagt tcgtcggcga cgagaacccc gcctaccgcg acctgctgcg ccatgtccgc
    12421 gcggcgctgg gctacggcgt cgtcctcaac acgagcttca acatccacgg cgagcccctc
    12481 gtctgttcgc ccgacgaggc cgtccggatg ctcgtggaca gcggcatccg ctacctcttc
    12541 ctcgacgact ggctcatgga gaatctgcat gcgaaaatgg actgaaaccc gggccgtccc
    12601 ggcggccgtc ctgatcctgt atgccgccct gatcggcgcc ttcctctatg tcttggacta
    12661 cgcggccccg ccgcggatcg aaacggccct cgtgaagaca atgccctggg tcctccgggc
    12721 gaacatgctt ttgctggtcg cgggggcgct ctggtgctgg ccggacgtcg cggccttccg
    12781 ccgggagatc tttccccggc gtccgtcgcc gccgtcgccg ccgtcgtcgc ggccctcgcg
    12841 gcgccccaac gcccgcgcct cggccctcgc gctcctcctc gtcgccgcgg ccgtgctcgt
    12901 gatcttcgcc gccccccgcg ttcaccggat ctactacgac gaggacatct acgccaacat
    12961 cggccagaac atcgcgctga cgcaccaggc cgcgatgtgc aactacggca cgttcgagta
    13021 cggcgaatac ttcccccact ggctcctgat gaataaggag ccgaccggct ggccgttcct
    13081 gatgagcctc gtgttccagg gctttggcgt ggacgagcgc tacgccttcg gcctcaacaa
    13141 cgccctgtac ctgggcggca tcctcctcgc cttcttcatc gcgtaccggt tggcgagggg
    13201 cggggcccgc gctccatctt catcgccgtc ccaaacggcg cgccaggcca tcggggaggg
    13261 cgtgggttcc cgcccgtacg gaaacgacct cgacataccg ttttttgcgg ccttcttcgc
    13321 ggcgctcgcc tatgccatca ttccccacaa tctgctctgg tcgaacaccg ccgccgtgga
    13381 gccgtccgcg gcattcttca cgggcctcgt cgtcctgctc ctgatcgtct acctgcagac
    13441 gggaaggatg cgccacctgt tcgtccttgc ctgcgcgatc cccttggctt gccagatgcg
    13501 cgcggagtcg gcgctgatcc tcttctggct cgtcgcggcg gccttcctcc tgaagcccgg
    13561 cccggccgcg ggcgcggagg gcgcggggga cgcggagggc ggcgccccgg cgagcgcctg
    13621 ggagcggcgc ctcggggtct tcgcccgtcg cgaggtctgg atcgcggggc tgttgtgcgg
    13681 cgtgctcctc gtgccccacg ccctgcacct gttcgtcatg aagggccagt cctggggcgc
    13741 ggagggcgcg aagttcgcgg gatcgttctt ccggccgaac ctcgccgtca acggcctgta
    13801 ctacctcgtc aacgcgaagt tccccgcgat cctcacgctg ctcgcgcttg ccgggctcgc
    13861 gtgcggctcc gccccatggc gtgcgcggct cgcgacgctc ctgtggttcc tgccgttctg
    13921 ggggatcttc ctcttttttt acgcggggag ctacggttac ggggcggacg tgcgctttgc
    13981 ccttgtctcg ttcatgccgc tcgccgtcct cgccggactc ggcgcgggcg ggattctggg
    14041 agtggccggc cggttgtttc cgaaggacct ggattcgcgc cgcagccacg cgccggacgg
    14101 gcctgccgga ggcgggaacg acgggggaac ggtcgggaga ggcgaggccc agtgcgcggc
    14161 acgggatgcc ggcggccggc ggcggttcgg aaggacgggg gttgcggccc tgctcgtgct
    14221 ggtcatcctc ttctgctggc tccccttcct tccgctgatc cggacggtcg gccaggaggc
    14281 gtgggcggcg cgctacgatc acctgtacgc cgggcgcttc gcaaagatga tcccggagcg
    14341 ctccatcgtg ctcacgcaca accccacgat gttcctgctg tgggggcagg gggcgatcca
    14401 ggcgggcgcc gggcttgaca acccggacgt cgtcgccttc ctgatgaaaa aatacccggg
    14461 ccacgtatac ttccacgaga actactggtg caccgtgccg ggcgcgaacc aggaaatgtg
    14521 ccgggcgatc cgggcgaagt acgacctgcg cgaggtcgcc gccttcaccg agcggaacca
    14581 ccgctacgcc ctgtatcaaa tcaaaagata gggcgccatt ccccgatgat cgggaggccg
    14641 acgccctgtc tttcagtcac cctgagcgta tcgaagcgag ccggggtctg ataggctcac
    14701 cgcgacgcat cacgcctgtt tgcgacgccg gatcacgacg acggaggcga cgacgatcac
    14761 gaggacggcg ccggcgccga tcaggaacgc cttgaacagg acgggcttct gcgtgacgcg
    14821 gatcgtcccc tccagcaggc gcgcatcgtg gcggccctgc gcctcgtacc agacgaccgc
    14881 ccggtaaggg agatcccgcg ccaccgtcgg ggcgaagttc agcgggagtt tgacggtttt
    14941 cgtttcgccc gcggccaccg ggtaggcctt ctccggctcc gccgtggtca cgccgtccgg
    15001 caggaagaag gagacgacgg gttcgagcgg cgcgccgccc gtgttcttca ggatcagcgt
    15061 gatatcgtcc ttcgtgttga gggggctctt ggcgttgaat tcggcgtcgc tgacggcaac
    15121 ggtcagcggc gaggccgttt gcaccccggg tgccgccgcc ccgaaggcca gggggatgaa
    15181 gtggtggacg gcgtgcgtgg cgcccgcccg gtcttcgaag ttcacgcgcg ccgccaggat
    15241 gtaatggccc ggtacaagcg cgcgcgtgtc gagggtgcgc gtgcagcgga ccgtcttgcc
    15301 gggtgcgttg tcgcccagcg gatcggaccg gtccgcaatg aggccgagga acgtggccac
    15361 ccgaacgtgc aggacctcct cgttgcccgc gttcaggatg tcgtaggaca ccgtcgtctc
    15421 gcccgtggac aggatcgacg ccgtcgcggt gacgatcgta cggatctccg cggtcgccgg
    15481 ggccgccagt agggcgattg cggcgacaag gatcgcgatt ctgcgtgcaa acttcatgat
    15541 tctccctcca tcatccgggc gtgtgcccgg ttctcgtccg ttcattccct ccccgatttg
    15601 atcaggaagg ccttggtttc gtgggcgtcg aggtccgcga ggatcgcgcc gtcgacgacg
    15661 ggatggaccc ggccgctgaa gatctcgacg gtctcgccga ccgccccgat cttcaggcgg
    15721 gccgaaacgt tcgccccgat cgtgttgacg gcgatgagga gcagaccctc ctcccgctcc
    15781 ttgacgcagg cgtggacggc cggccgccca cgggggtcca cccggttctc cgagcgcatc
    15841 tccacgtcca ccgcccgctc cgcattcttc acgaggagcc acggatacac gcggttcagg
    15901 cggccgacga cgcgtccgag gcgcgcgcgc ccctcctccg tccggcccat caccgaccag
    15961 gtatagaaga agatcccgcg gctcccgtgc acgatggaga gaaacgccag gcagtccatc
    16021 tgacgccagc cgggcaggga gggccaggcc tcccccttgg cgaaggcctg gatgaccgac
    16081 atgaggcggt cgcgccccgt gtccgcggcc gcctcgtcca tggcgtcgga aagccacgtc
    16141 atggggcggt ggggaaaggg gtactggtcc atcatgaagt agtccgccgc cccgaggtaa
    16201 tcccgggcgt gctgcggccg gacgatcgcc atcgccgtgg cgacgccggg aaagcggtcc
    16261 gcgagcaggc ggcggacgtc ctcggcgcgg gtggcgggta cggagcgcag ttccggctcg
    16321 tccgcgacgt agaagccgag caggccgccc ccgcggacgc gcggcacggc gtcgagacga
    16381 tccaggtaaa ccttcaggtg ctccgggtcg ccgggtgtgg acaggacata gcggagccgc
    16441 tcgttccggg ccgacgcgat cgccttcgcg agaggctccc cctcgccgcc gaggatgacc
    16501 gtgttgaacg cgattgcgcg gacctcggcc aggcgagacg gatccgcccc gtaaaccccg
    16561 atcgggaaga atccggcgat cgagggcggt ggcgtccaga gggcggcgtt tggggccatc
    16621 tccgtcggcg ccgccgcgag gccgtgcgtg cccaggcaaa accgctgcgg gacgccgccg
    16681 gcggacgcgg gttgcgcgcc gaagcggaag gtcggatcgg cgatccgctc cggacaggtc
    16741 acgcggatgc ggaacgtctg cggggcgccc gtcctgcagt gcgcgttcag gtggaatttt
    16801 tgtccccaca gggagatcca cggatacgtc cggtcgtccc atgtgtcgcg cctgaactct
    16861 ccggagaaat ggtaggtccg gcccgcctcg caggcgggca ggggcgtcgt ccaaaccccg
    16921 tcgtcgggcg ctgttgcccc gggataggcc ggcggcctga tgcacacctc ctccgtccgt
    16981 gccgccccgg gcgttccgag tgccacgacc acgatacaga tgatccagag gcgacgggcc
    17041 tgcgtccgga ggttcattcc cgcgcacccc ctgcttcacc gcccagggcc tcggccaggc
    17101 gggcggggtc gtcgggatag aggttgcgga cgaagttcgt gatctcggcc acgaaattcc
    17161 tgtccggctt cattccgtcc tttgtcttcc gtccggtcag cgacagggcc tggccgtaat
    17221 gccacgacgc cttgagccag gagagctccg gcgggggcga ggacggtccc gtgttcttcg
    17281 accggacggc ccagtagttg cccatgtcca cgtggaggtg agggttgatc gaccccgtgc
    17341 cggcgtagta cgccgcccgc tccatgctcg catcggcgcc gcgcatccag cgggtcatgt
    17401 agtcgtggcg gtcccacagg taggtgtact cccaggcgag gcgcacatgg gtttcggcgt
    17461 tgagcgggtt acggcggatc gcgtcctcga gcgcggcgat gaccggtccg tgcgaggcgc
    17521 gccaagcggc ctgcgccgtt gtgagcgacg gcgcggcggg tcttgtcttt gccggggccg
    17581 catccggtgc ggtcgcacct tcatggccga cggcccctgc ggcgggtttg gccggagtcg
    17641 cccgcggccc ggttgtttca gacgttgccg cttcccggac cgcgtatgcc gcctgcatca
    17701 agcggtcccg ccggtccatc agttcccgcg cgagtttcga acggtattcc gcgtttgccc
    17761 cgtcccaggc gatggccgac cggatgttgt ccagcgattg gtgctcctcc aggttcatcg
    17821 tgagattgcc gacggtggcg cagtgggcct cggcgacggc gtgccggacc gtccaaagac
    17881 ccgtccacac gatgaggacg atcacgaacg cggccgcgat cccgcccgct cccgccatcc
    17941 gaaggtgtat gcgcctccag cgcatcacgt cctcacgccg ctgccgctcc aggtgcagcg
    18001 cgctcaggcc gatcgcgcac acggcggcga gcatcatgaa gttcgccggg atgtgcaggt
    18061 tgaagtcaaa aagcgagtgc gtcccgatgg atgagagcgc ccccaccggc gcaaggccca
    18121 ggcagacggc gaaggggtcc ccgcggatcg accacgtccg aacgaggcgg tacacgaacc
    18181 accccagccc cgcgatcagt gcgagcatcc cggtgtatcc cgcctccgtg aagaactgca
    18241 cccagtcgct gtgcagaaag tcgatgaagc ggcccttgtc ctccggcgcc tggtacttgg
    18301 ggtaggcgtg gcggaagttg ccgacgccga cgcccacgag cggatagtcg tcgtgcagca
    18361 ggaccgcgcg ttcgacatag cgcggtcgcc ctgcctggtc gaagtccagc ttctggaagc
    18421 gttcgatcgt gtagtccatc ccggcgaatg cggcgtacag gacggttacg ccgaagagcg
    18481 ccaggacgat cccgcccttc gcccggtgat cccgccgtgc gaggaagaag acccccatga
    18541 cgaagagacc agccagcgcg gagacgatcc cggcacgtga ggcggacagg atgagcgcga
    18601 cgcccatgat gacgccggag aagatgacga ggatctggcg gagggccttg ctctcgacgg
    18661 agaagaggag gatgaagcgg tccttgaagg tccatgcgcc gcgggcgtgg acgatccggc
    18721 gccgcccgcc ggcgacgatt gcgccggcgt aggcgatggc cagcgcgatc cccatctcca
    18781 tgaagccggc aaaatggttt cggttgatgt aggtgccgct gacgtccttc gcgccctggg
    18841 agaacttctt ccaccaaaga atgtaggggg agccggaata ggtctgggcg atgccgtaca
    18901 gggcgttggc gaccgcggtg gcgagcaggg cgacgaccaa aaactcgatg cgccgccggg
    18961 aattcagggt gcggcagagg ccaaagaaga gcaggccgta ggcggtccag cggatgaggg
    19021 acatgcggac ggggtaggcg tagggggcca gcgtgaacca cgcgttcttt agcgagggat
    19081 ccgcgacgga cgcggtggcg gggagggcgc tcagtcccgc agcttttgcc tctgccgaca
    19141 gcttcgtcag cagccatccg gggagcggtg tcatctgcac aacgagcagg aggaccatca
    19201 ggacgaacag cgcctccgcc cccgtaatgg tcagcgggac cgcccggttt ctcttgaagg
    19261 cgggaatcca gtcccgcgcg atcagcagca ggctcgccgc caggatcagc aggaaaaccg
    19321 tcgcgtacgc ccaggtgtgc accgccccga aaaaaacgat gcttgccacc agcacgaaca
    19381 catatatccc gaatgccgtt ctttccatca atccgctctt ccggaggacg tctggtcttt
    19441 gatgcgcttg acgtggccgc ggccgaggcc cttcacctcg cagccgagct cgaagtagcg
    19501 gcggatggcg gcgtcgtcga ggaggccgaa gcagtcgatc acggcgacgg ggcggccggc
    19561 catttccacg atctttgcgg ggtcgagcgt gcggtaggcc gcgtggcgga cggccaggac
    19621 gacggcgtcg gcgcccttga gcgcggccga gagatcgggc tcgatccgga ggtccgtcag
    19681 gctctcctgg ttccggaaga agcgcgccca ggaatggccg ggggcggggt aggtgtcctg
    19741 cttctccagc tcccaccagt gcttcacata cgggtcgtgg acgccgatct ccgcgcccat
    19801 ctcggcgagc ttgcgcacga gggcctcgct tccgctgtag cgcgtgtcgc ccacgtcctc
    19861 gcggtaggag gcgccgagga tgacgacctt cgccgcggcg acgatcttgc ccatgttgcg
    19921 cagggcgtcg cggacgagtt cggcggcgtg cagggcgcgt gtgtcgttga tgtcgatcgc
    19981 catcggcgtg atcttgaaga tatcgtcctc gaagcccatc aggtggcggt aggaccagac
    20041 gcccaagccg ccgtccttcg gcaggcagta gccgccgatt cccgggccgg ggaagatcat
    20101 gttcgagtgc gtcgggcgga ccttgatcgc ctggatcacc ttcatgaggt cgacaccgtt
    20161 gcgctccgcg aagaggctcc actcgtcgag gaaggcgagg atcgtggcgc ggtaggagtt
    20221 ctcgacgatc ttgcaggtct cgctctcgat ggggcggtcc agcacggtca gcgggaacgt
    20281 ctcgacgttc aggacctccg agagaaacgt ggcgacgcgg gtgcgggagg cggcgtttac
    20341 gccgctgcag acccgccaga agtcgcggat ggaggccacg tactcgcggc ccggcatgac
    20401 gcgctcatag gagtgggcca gcagcggctc ggcggcgagt ccgcggcgct caaaggcctt
    20461 cttcaggatc gggtaggcga cgtattcggt ggttccgggg gggacggtgg tttcgatcag
    20521 gacgaggcag tccggagaga tgcgctcgcc gatgacgcgg aggctctcct cgagggcggc
    20581 gatctcggcg cggccctggc gacagttgcc cagggtctcc ttgaagtagt cgcactggac
    20641 gtcgacgacg acgcaatcgg cgagcgacaa ggcgtcgtag gtgtaggtgg cggtgagggt
    20701 cttcttctcg aggacgcaac gggcaatgag gggggcaacc tcggggtcct ccgcctcgac
    20761 gggggcgatg ccgcggttta agtacgggat cttccagtag gagcgcgggg aggggcgctg
    20821 catgccgatg acgaatttag cgggttcgcc ggggacgggg gcatgggcgc tttgcccggg
    20881 gcgcgtgagg gccggatcta tcttttgggt ggttttgggg gggcggcgcc ctccgctcgc
    20941 ggtgccccgg cgcggggcct tggggtccgc gtgttgaacc acgggtttcc ggacggcgtc
    21001 ggcgacgatg ccggccatga ccgccccgac gaacccgacg cccatgacga cgacgatctc
    21061 gcggcccgcc cggcggtgcc gggcgacgag cctcttgagg cgtctgaatt ccttgacgta
    21121 atcctcgggt ttggggagcg ggaagacctc gccgttgggg gactgggaaa cgcgctcgtt
    21181 ccgcgttttg gggttcagac tttgcttcgt tcgcttcgct ggcatcagat cggtattcct
    21241 ttccttccgg tttgtcttgg gatcggcacg ttcgagaggt cgattgttaa tatgagtggt
    21301 tcgttcaaga atcggacata accctgattt tcaagattcg cctcccgagg gttgtgtgtg
    21361 tcaaaaatgg gtgagcggcg ggcggccgat cgggtagacg ttgaagccga tggcgtggag
    21421 ccgctcgtgg tcgaggatat tgcggccgtc gaagacgaag gccggctgga ccatcgtgcg
    21481 gaagatgcgc tcgtagtcga gaccgcggta gagcggccat tccgtcatga cggtgagggc
    21541 atggcagccg gccgcggccg ccacggggtc cgtggcgaac gcgatcttcg gacccgcgcc
    21601 ggtttcgaac agggagccgg tgccgcggtc cgtcagtccc tgcgcccaga tcctggcgtt
    21661 ggagagggcc tgcgggtccg tgatgacgac ctcggcacgc tccgcgagga ggcgctccgc
    21721 gacgaagatc gccggactct cgcgcgtgtc gcccgtgtcc gccttgaacg cgaacccgta
    21781 gagcgcgatc cgcttgccgg ccaggctgtt gaacatcgcg gccatcatgt tcaggacgaa
    21841 gcgctccttc tgatactcgt tgatggcgac gacgccctcc cagtacgccg cgacgtcgtc
    21901 gagcccgtag gcgcggcaga ggtagacgag gttcaaaatg tcctttttga agcaggagcc
    21961 gccgaagccg actgaggcgt tcaggaattt ggggccgatg cggctgtcca tgccgacggc
    22021 gcgggcgacc tccgtgacgt tcgcctcggt tttctcgcac agggcggaga ccgcgttgat
    22081 ggaggagatg cgctgggcga ggaacgcgtt cgagacgagt ttggagagtt cggcgctcca
    22141 gatgttcgag gtgaggatgc gttcgcgggg aacccagcgg gcgtagattt cttcgaggac
    22201 gtggcgggcg gcgaggccgg tgccggtctc gcgcgagccg atgaggacgc ggtccggatt
    22261 ttcgaggtcg cggatcgccg tgccctcggc gaggaactcc gggttggaca ggacgtcgaa
    22321 gcggacgtcg ccgccgcggg agttcaggat gcgctccatg gcaagggcgg ttttgacggg
    22381 gagcgtgctc ttctcgatca cgatcttcgg ggattcggcg tactccagga tttcgcgggc
    22441 cgtcttctcc cagtactgga ggtccgccgc catgccggca ccggcgccga aggtcttcgt
    22501 cggggtgttg acgctgacga agatgatgtc gttcgcgcgg atcccgccgg cgatgtcggt
    22561 gctgaagaaa aggttgcggc cgcgggcggc gcggacgacg gcgtcgaggc cgggctcgta
    22621 gacggggagc gtctcggagt tccaggccgc gatgcgctcc gcgttgacgt ccacgaccgt
    22681 gacccggcag tccggacatt ggtgggcgat cacggccatt gtcggcccgc cgacatagcc
    22741 ggcgccgatg cacaaaatct tcctggcggt cagatcgtta aaatccgttt tttcccccat
    22801 gggtgttctt cctgccggtt gtcatgcggg gccttcgcct ttaaagaccc ggtccccgtt
    22861 tgtcgttgat ggattcgaaa taacgaatcg tctgccgcaa tcccgcttcg agcgggatgc
    22921 tgggctccca gttcaagcgg gagcgggcca gcgtgatgtc cggctggcgg tgcgtcgggt
    22981 cgtcctgcgg gagggggcgg tggacgatct tccccggggc gcccgtcagc cgaagcaccg
    23041 cctcggccag ttcgcggatc gtgaattccg tcggattgcc catgttcacc gggccgatga
    23101 agtcgggcgg ggaatccatc atccggatca ggccgtcgat cagatcgtcc acgtagcaga
    23161 aggagcgcgt ctgcgtcccg tcgccgtaga gggtcaggtc ctcgccccgc agggcctgga
    23221 caatgaagtt gctgacaacg cggccgtcgt ccggtgccat gcgcggaccg taggtgttga
    23281 agatccggac gatcttgatg gccacgccgt tctggcggtg gtagtccatc atcaggcact
    23341 cggcggcgcg tttgccctcg tcgtagcagc tccggatccc gatggggttg acgcggcccc
    23401 agtaggcctc gggctggggg tgcgtttccg gatcgccgta cacctccgag gtcgaggcct
    23461 ggaggatttt tgcatgcacg cgtttggcca gcccgagggt gttgatcgcg cccatgacgc
    23521 tcgtcttgat cgtcttgatg gggttgacct ggtaatggac cggcgaggcg ggacaggcga
    23581 ggttgtagat ttcatcgacc tccaggtaga tggggttgat gatgtcgtgg cggatgaact
    23641 cgaagcgcgg gtggccgagc aggtggcgga tgttgtcctt gctgcccgtg aaaaggttgt
    23701 cgaggcagag gacatcgtgg ccctgggcga gcaggcggtc gctcaggtgg gagccgagga
    23761 atccggcgcc gccggtgacg agaatgcgtg cggtcatgat gcgggtcctc cgcgggcttt
    23821 gggaatcagg tccttgaatt catcgttatg cgccatggcg tctgaccgcg cgattcgcgc
    23881 tcgtgtcgtg aatccgcgcg gaaacctctt gccgggtccg ccccccgtca tttgaagaag
    23941 cggtcgaaat aggaccgcag gccgcgtttg gcggagcgat agggcagggg atcgtcgggc
    24001 tcgacgaact tgccctccag gatgcgtcgg gggatctcct ccgtcatgcg gcgggcggcc
    24061 ggctcgccgg cgatcgaggc gacgacgtcg cgggcggccg aaagcttagg cgagcggcgc
    24121 ttgagcccgt gcgcgtccga gaccaggatg tggacgaggc ggtgctcgat cagttcctgg
    24181 gaaaaatcga ggatttcctc ggagaactcc tggacgaggc tcgccgcggt gatctgcgcg
    24241 aggatgccca tttcgaccca gcggaacagg cgcatggggt ccctctgaag gacggggttg
    24301 cgctcgacgt ggctcaggat cggcttgatc ccgtcgagtt ggagcgccca gaagaagtct
    24361 tccaggttgg aaggcaggga ttcctcgggg agctcgatca agacgtattt cccgccgtcg
    24421 atcagggtga gcagctcgcc cctccggatg cgctccggga tgccggtgtc cacgtgcaac
    24481 tcggcgccgg gccagagcgt cagcgggatc tgcgactccg ccaggcggtc gcgcagatcg
    24541 gcgcaggcgg ccaggatcgc cgcgcgcgtg ttttcgtatt tcccgaggac ccagtgcggc
    24601 gtgcagacca tgtcggtgac gccgtccgca acggaggccc gagccatgtc caggctgcgc
    24661 tcgaggtcac gtgcgccgtc gtccaggccg ggcaggatgt ggctgtggag atcgatcatg
    24721 atgggaaatc ctcttggttc ggctccgctt tcccggttcc gcccgcccct tggcgggcgt
    24781 ggaaacgaga gcgtcttaaa aaagtccgcg acgcgagggg tgcacgcggc gtgatcggga
    24841 ttttgccggt atgcgccggg cacgatactt aaaagcgtac atcaggttta tcatctcagt
    24901 tttttgagcg actgtaaaca ggaatgcgaa cgctaattca atcttttttg attgtcaata
    24961 tgaaacgttt cgattttggg ggaagatgag gatcctgacg gtgaggcgat ccggcggcct
    25021 gaaaccgcga cggccgaaaa attcgccggt ggcaatgcgg gggacgttct gttatgaaga
    25081 aaagaaatgt cgagggtgga tggaggggat gaatccacaa gggaatcggg agaacgggtg
    25141 cctgttcatc gtcggcacgc ccatcgggaa tctggaggac atcacgctca gggccatccg
    25201 ggtgctgaag gaggtccgcc tcgtcgccgc cgaggacacg cgccacagcc ggaagcttct
    25261 ggcggcccac ggcatcacga ccccgcttat cagcctccac gagcacaacg aggcggcccg
    25321 ggctgggctc ctgctcggga agcttcaggc cgggcaggac gtggcctaca tttccgacgc
    25381 cgggacgccg gcgatctccg acccgggtgc gcgcctggtc cgagcggctc tcgccgcggg
    25441 ggtccgagtc gtgcccgtgc cgggggcctc cgccgtcgcg tcggccctga gcgtctcggg
    25501 gttcagcgcg gacgccttcc ttttcgccgg gttcctgccg gcgcgggcgg ccgcgcggcg
    25561 tttggtcctt gcggccctga aaggcgagcc gcggacgctc gtcttctatg aatcgccccg
    25621 gcgtttgacg gccacgctga aggacctggc ggccgtgttc ggagaccggg aggccgtgct
    25681 ctgccgggaa ctgaccaagg tcttcgagga ggtcctgcgg gggccgctga gcgcgcttgc
    25741 cgcaaaactg gagacggggg cggtccgggg ggaggtcacc ctggttgtcg ccggcagcgg
    25801 cggggtgaac accgagacgt ccgtcgacgc ggcggatctg cgggagatgg cggcgcgcct
    25861 cgcgcgggag ggcctttcgc gacgggaccg ggtcgagatc atcgcccggg agacggggct
    25921 gccccggcgt ctcgtctatc agaagatcgg cctggaaacc taggggggcc ggacccgcct
    25981 caaaaggcct tgcgtggggg atccggcccc cgtcgacgcg gcaaaaaata cggttgaagt
    26041 gtcggcgcgg ttgtgttaac gcggggcagt acggccggcg cgacgggaga cacgcgccgg
    26101 gacagggggg acaatgggcg agaatgacgg tggattcccg ggcgcgatgg cggacggggg
    26161 aaccccggtc gggcggagac ccggccccgg cgaggtaatt tacgatctga ccgagagggt
    26221 cgaggggcgt gtcggtcctg cggcggccga gattgatcgg gcgggggggc ggcgcatctt
    26281 cgatctgacg gaggtgatcg aggaggggcc ggtctttccg cctgaggcgg aggccttccg
    26341 cgaggagatg atccgccggg tcgagacggc cgccggggcg attgcccgtc agatcctgcc
    26401 cgccgtcgcg gagcgcattg cccgggaggg ttttccggag atcgccgcgc ggatcgtccg
    26461 cgaagaaatc gagaaactga agagcgaaac ggaggatgca ccttgagcgg aaggctcgct
    26521 gaacagggcg gacgcgccct ccggcatcgg cgggacgacg cgcccccaac cgtccacgtc
    26581 gccgtgccga tggatctccg gcaccgcagg acggaaggcg cagccgcact cgcccgcgtc
    26641 gccgtgcggc tcctcgcggt tgcgctcgcc gtactcctcc taggcgcctg cgccgggaaa
    26701 atcccccaca cgctcaagcc cgagtacaag gatgcgggca tccgcctgat cgcagtgctg
    26761 cccgtgcagg gggagaacat ggacccgcgg ctggcgagac tcctgaggga gaaggtgctc
    26821 atgggcttgt atttcaaggg ctacccgaag attcccctga ccctggtcga cgaaaaactc
    26881 gcgacgttct accaggcgca gacgggggcg cagggtgtcg tggcgccccg ggtggccggg
    26941 gaactgctcg gaatcgacgc ggtcctgtat atctccctcg tcgaatgccg aacggcatac
    27001 cgggcgctct atgcccagac cacggtggcc gccaggctcg aacttcggag cgcgaggacg
    27061 ggggagacgc tgtggcgcgt gcaggaccgc gaggcggcgc gcaattttgg gttcacgtcc
    27121 gaggaactgg agatgaagtc gtccgaggta tgtgaagcgg cgatgcagac ggtcgtggat
    27181 cgcgctctct acgccctgcc cgacggtccc gatcgcctgg gatgaccccg gatgcgaaac
    27241 cgcacccgaa acgtttccag ggttggaacg ttgcacatca attttaaaga cctgaccccc
    27301 cacaaggagg cgattatgaa gaagggctat tggatcggcg gaggggttct ggccatctgc
    27361 ctcgtgttct gggtgacgac ggtaacggcc cagggcatcc ggacgatgca ggtgggcaag
    27421 ggcggcgccc gggtgaccgc catcgacggg agcgcccagg tccggatcgg cgggcagggc
    27481 gcgccgcggg ccctgcgcgt gggcgagcac ctggcgggcg ccgacgaggt cgccaccggt
    27541 tcccggtcgc gcatcgagat ggcgctgccg gacggttcgc gcatccgttt ttcagagaac
    27601 acgcgcttta ccctgctcga ggtggagccg gggagctacc gccgggtgcg gagcatccgc
    27661 gtgaacgtga tcgtcgggcg cgtctgggcg aaggtggccc gggcgatcgg cgcccggggc
    27721 cgcttcgagg tgatcgccga gaactccgtc gccggtgtgc gcgggacggt gtaccgggtg
    27781 gacgtgaacg aggacaagtc caacctgatc cgggtctacg acggaaccgt ccacgtgtcg
    27841 ggcagcgccg aggccccgac gaagccgccg gcgccggccg tcatcggccc gccgacgaag
    27901 gtcgaaggcc cgaagtccat cccgggacca cgcgtggtgt cgatggagga atgggtgtac
    27961 atcctgaaat ccatgcagca gatcgtggtg cggccggacg ggtcggcgga cgagccgaag
    28021 gatttcgcgc ccgaggagga ccgcgatccc tgggtcgact ggaacagggc gcgggatcag
    28081 gaggcggaca gggagggcgg tgcggacgcc gcaccccaac cccggggcgg cgtcccatgc
    28141 gagaattgaa ttaaaagatg aacatcccga acctgctctc gctcttccgg atcatcctgg
    28201 tgccggcggt ggtgatcctg ctca
//
LOCUS       DAOOLJ010000021        26404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_1689_length_26404_cov_16.484231, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000021 DAOOLJ010000000
VERSION     DAOOLJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 26404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26404
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1689_length_26404_cov_16.484231"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1056)
                     /locus_tag="PLW16_05430"
     CDS             complement(55..1056)
                     /locus_tag="PLW16_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPB70383.1"
                     /translation="MSASLSIVIFVIVLALIFDFINGFHDSANSIATIVSTKVLKPYQ
                     AVVWAAFFNFAAIFLFDAQVAKTVGTGIVNTSIVNHTLILSALCGAIIWNLITWWFGL
                     PSSSSHALIGGLIGAGLMRGGFNVLIWQGIIKTASFIIISPLIGLILGLFLMVVVLNL
                     SRHSNVSRSNRIFKKLQLFSAAIYSISHGMNDAQKTMGIIALVLYSGGYLGPTFHIPF
                     WVVVSCYTMLALGTMSGGWRIVKTMGTKITKLQPMGGFCAETAAALSIIGASFAGIPV
                     STTHTITGAIMGVGSTRRLSAVRWGVAGNIVWAWLLTIPVSGVLSSGIYFLLATLFP"
     gene            complement(1071..1517)
                     /locus_tag="PLW16_05435"
     CDS             complement(1071..1517)
                     /locus_tag="PLW16_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70384.1"
                     /translation="MRAFDEEDRRVKEELQSLSSMTATLGLVVPEISEAITVHEKLSL
                     QYRDALKDYNRADLKSAVLVDQRVRGIDREPTDQIDGIVAKIEMQADKRLTAVETLSK
                     THLEVYQSLSFFLIFLVLVGVCFGIFNAWSILKDLPPEENDRNAEG"
     gene            1518..1844
                     /locus_tag="PLW16_05440"
     CDS             1518..1844
                     /locus_tag="PLW16_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70385.1"
                     /translation="MAFIEVLIVAAQKDIFPFLDLPFEVNLCLARRIDGLDHHLPFRN
                     TLMKALVGFHNGYQTEQPDHHGQDGEDPDGDDDPPPNGNVLENRIGFFHKSKTSLADV
                     GMYQTA"
     gene            2276..2551
                     /locus_tag="PLW16_05445"
     CDS             2276..2551
                     /locus_tag="PLW16_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70386.1"
                     /translation="MPDYWIKISETEEEDRKQHHYLTTAKDEKEARKAARDFMKRFID
                     DDDDPEEIENGYAFYNKAIFVRLESLKETTKEQFKEFLLKIHTINMA"
     gene            complement(2665..2994)
                     /locus_tag="PLW16_05450"
     CDS             complement(2665..2994)
                     /locus_tag="PLW16_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70387.1"
                     /translation="MPVYLKFLLLLAAFVAFVVLVLYLGGLSVRRVCFKIIAELEEAG
                     AYSAARAIPIQDERKNIFRVGTGNIRPKALNVLLGDKIVVRTGNGNYHLDRDALARMK
                     ARLKADR"
     gene            3267..4106
                     /locus_tag="PLW16_05455"
     CDS             3267..4106
                     /locus_tag="PLW16_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB70388.1"
                     /translation="MTFIQAIERRISCRTYRQLPLEPEMIEGLAQALLSNTKGPFGSR
                     VRFALIRSDRLDQIDLKPPGTYGVIKGARQFIAGAVLDNPRAMEDYGYCMEKTILAAT
                     AMGLGTCWLGGTFHRTGFAGRMNLAEGELLPAVSPVGYASDQRSLRERVFRFGAGSDR
                     RKPWEDLFYERGIVTPLTKDSAGRYATPLECVRIAPSAANKQPWRIIRDRGAYHLYLK
                     RTPGYDRIFATIRIQNIDMGIAMCHFELSARETGLDGGWTVEAPGIPSGDLEYRVSWI
                     ATS"
     gene            complement(4164..4961)
                     /locus_tag="PLW16_05460"
     CDS             complement(4164..4961)
                     /locus_tag="PLW16_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB70389.1"
                     /translation="MDEQPLLLEKKGHVATLIINRPDKKNSLTPLVLFALADQFEQLS
                     RDAGIRTVVIRGSGDKAFSAGYDLSAIPTDLSPDLFDALKKKNPLEVGFAAIEAYPYP
                     VIAMIDGFALGAGCELAITCDLRVASDTSRMGIPPSRLGVIYHPAGVQKFINVLGLAN
                     AKEAFFTGRYYDIHRAQAMGMVHYVVPKADLPAFTDRLAEDIAGNAPLALKGHKHIFN
                     KLLHAQGIHPGDQAEIERLIQEALNSEDLKEGTLAFFERRKAEFTGR"
     gene            complement(4989..5930)
                     /locus_tag="PLW16_05465"
     CDS             complement(4989..5930)
                     /locus_tag="PLW16_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70390.1"
                     /translation="MGTIEKLHAHLFAIDQRWLDHAKFASTYLLVGDRVALFDTGPTT
                     VIEHVLDAVREIGYEPEEIDCLVFSHIHVDHAGAAGSLCRRYPRLRALAHERGVPHLA
                     NPSKLVESMRRVFGENAERWYGAVEPVPEAQLAPLADGDVIDLGRGVRLRVVHTPGHA
                     VHHLSLFEEKSGALLAGEALGVYFPEAEVHFPSTPPPEFDLVRAVGSIEKLAALNPQQ
                     VLYAHFGPVPNAETSLRAAREVLVDWGRIVHHALQGQGDIAHVSEKLSEAALKRIAHL
                     SDRPELYGRYKSLVEYRSRYTCGPGYVRYFKQGGSVL"
     gene            complement(6050..7378)
                     /locus_tag="PLW16_05470"
     CDS             complement(6050..7378)
                     /locus_tag="PLW16_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HPB70391.1"
                     /translation="MEATRKLAAYVAGTQFQDLPAEVVREAKLCFLDWLAVTLAGARE
                     ADIDSLAQVVALVGGEPQASVIGRPVKTSILNVALLNGMSSHVLDFDDTSVEFLGHPS
                     VTLFPGILAAAEWRRESGRAFLAAYVLGYETGCRVALAASPNHYLAGWHGTSTIGHFA
                     AAAGTAKLLGLDADQLIHALGTAGTQASGLKAVFGTSCKPFHAGKASFDGLLAALLAQ
                     RGFTSTGDILEGQKGFWEMFSKDWKSEDALQGLGVQWYILRNNYKFHASCYGTHAPIE
                     AALALKRVEGMEARQIRAMDIHVSRPMLDVAGKERPESGLEAKFSIPYSVANALLNRD
                     TGMNAFTEERVHAPETVALQQRIRLVPDDTLAPFEAEIRLDLEGRSLRQTFNMLEHVM
                     DPREKEDKIVGKFLALGGLAFAPDRLERIVARVLDLEAEEDMAAWMALLS"
     gene            complement(7389..8963)
                     /locus_tag="PLW16_05475"
     CDS             complement(7389..8963)
                     /locus_tag="PLW16_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70392.1"
                     /translation="MKEAGVGDWVTKRHRLTPDKVAVIFEEREWTYRELNERVNQAAR
                     MLLELGLVKGARIAILANNCNPYLELLWACAKTGVVFVPLNTRLVSRELEYFITQSSP
                     EALFYGGEFSGMAKELKSWPGTGSIRNYICIDPPQDTTDGDYESWIRRFPVEEPPVAD
                     PPTMEDPQVILFTSGTTGRAKGAILPHRKTLWNTLNNENAFDYIVSSVIYILNLPMFH
                     SGGLFIATMPVIHKGGTLLIHRHFDPGMVLRDVERYRVSLLGGVPAMFQMIIAHPDFD
                     RYDLSSLKGVALGAQITPKSVLEQIRDRFKVEAVGQVFGATETSVCITLTPDRPKDLE
                     KVGSIGLPLFHGEVKVAGKTGEPVRPGSGEIGEICYRGPMTMLGYLNDPEKTRETIDP
                     EGWFHYGDMATVDEDGYIFVVDRKTDMYISGGENVYPKEVENILIDHPKIMAIAIIPM
                     PDEKWGECGLAIVNPYPGQSLTAGEVLEFGVGKVAKYKLPKEVIVTDKPIPQTATGKI
                     YKYKLIEQYVKGRQRP"
     gene            complement(9000..9410)
                     /locus_tag="PLW16_05480"
     CDS             complement(9000..9410)
                     /locus_tag="PLW16_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPB70393.1"
                     /translation="MNHRVIRVLVGKAGLDGHDKGALLLASRLRNAGMEVIYTGLRRT
                     VTQIVTTAVQEDVDVIGISILSGAHLSIATKLIARMEQEGITDKRLMMGGFIPEEDIP
                     HLKAMGVAGVFTQGAGFDEIAAFIRDAVASKDRS"
     gene            complement(9443..11176)
                     /locus_tag="PLW16_05485"
     CDS             complement(9443..11176)
                     /locus_tag="PLW16_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB70394.1"
                     /translation="MPLDQQTSDRIRAARTAWEEGVLKKNLQRMGLAASPVEFASPLD
                     IGEDDFLERVGFPGEYPFAAGRFAVPLHQVIWGRGKTLGGGKSIRHAFGYAGYGTAED
                     MRDFFIERGRTMARGGGPNIAFDLPTQTGYDSDHELAEGEVGRVGVAIDTLRDFETIY
                     EAFTGDTDLDRIASNWTINATTNVILAMYVALAQKRGIPLDKLRGTPQNDILKEFVAR
                     GTQIFPVGPSLRMTRDTFTYCHEHLPNMNTISICGYHIREAGATRVQAVAFAMANAMA
                     YFRVAVNAGMSVDEVAAKATFLNFGGGMEFLKEAACRRAARRVWAKIMRHRLGAVKPS
                     NWIYRELGGGLAGFWTCTRQRPLNNLVRVAIGAAFAAMIGEPPACEPPYDEALGLGHS
                     VEARQLAADAARIIMEECRLGDTPDVFAGSYYVEALTDRYEDEIFGLIDDIDRQGGAE
                     KAVESGWMKNEVLRSAEDFQQHLETGEATVVGVNKYTEADEIDVMTPRTSPYPVARRE
                     QAEEKQIAGLRRVKKERDPRAVQSCLEKIENAARDEGANLIPHFIDAVKAYATVGEIC
                     DTLRGVFGEAR"
     gene            complement(11269..12516)
                     /locus_tag="PLW16_05490"
     CDS             complement(11269..12516)
                     /locus_tag="PLW16_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70395.1"
                     /translation="MAREGKVFYGWWIVAATFIVLLIGLSSGFYTVSVFLEPLRRAFG
                     WSATQVSIGFTLAALLVGLLSPVVGVAVARFGVKKVQLYGATTVGLALLMLGSMQQLW
                     HYYALLIFMATGLASVSIVPSQTLISHWFNRKRGMAMGIIMSGIGVGGMVMVFVASQA
                     AASFGWRGAYRLLGLLVLGIVLPLILAVVKEKPADLGLDPDGIPLTATETQERASAFG
                     FTVAQAFKCLPFHVLAFLTIFYSLILGGMTQHAIALLRSLSVDQASLFWSLALGFSVL
                     GRLAFGGAADRISQKGLMIVIWGFHLIGLLSVVYLVDAAPLVWGFVIFYGCALGAFPT
                     LFPLMVGRKFGLDHFSKFVGVFGLIQILSLATGAILLGRMYDASGSYSGAVQVLLVVA
                     VLGTIAATVIGDPARPKTSVEQG"
     gene            complement(12577..13842)
                     /locus_tag="PLW16_05495"
     CDS             complement(12577..13842)
                     /locus_tag="PLW16_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70396.1"
                     /translation="MVTAMFADLAGSLLKERHEASLPVVQVAHPVGGISVTEARTRIT
                     DAVVRGVASSLTGEGASSSPGTPEGDGSAGADAGRILIEGGEADALEEFHQRGWTDGL
                     PVVLPTEDRVKAALRSVSGNPLDPIGCLLPRNAAATLLDIAINAVMAGCRPEFLPVIV
                     AAIEATQEADFNLYGVQTTTHNCGILVIVHGPISRTIGMNSGHNCLGNGNRANATIGR
                     AVRLVLQNVGGATPGELDKATLGSPAKFTYCFAENEEKTPWLPFRTALGFKAEESCVT
                     VVAAEAPHNINDHGSSTAASLLNTIAQTMATVGNNNLYVGGDTYLILGPEHARTIADG
                     GFSREGVQQVLYERARVATARITPEKLAELSSWGGYENRLEEWKEGIPLVRTPGDMRV
                     IVAGGSGKHSAWCPTFGATRSATRRIHER"
     gene            complement(13912..14205)
                     /locus_tag="PLW16_05500"
     CDS             complement(13912..14205)
                     /locus_tag="PLW16_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70397.1"
                     /translation="MTKPGFVHAYMPVGVSETGMSHPVHRLEALSGKRIGFLGNGKPN
                     TDVLLETLAAELKKQFPIEALHFRKQIPSLAAPDELIAEIRDRCDAVVLAVGD"
     gene            complement(14361..14678)
                     /locus_tag="PLW16_05505"
     CDS             complement(14361..14678)
                     /locus_tag="PLW16_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPB70398.1"
                     /translation="MENQPEIVCRRCGKCCLANLIAYVTDEDWERWRREGRDDILRII
                     AHEHAVWAGDHLISSDDGRYLHGCPFLQWDDDHYTCTIYETRPAVCRHYAPGSSEICT
                     QFR"
     gene            complement(14682..15674)
                     /locus_tag="PLW16_05510"
     CDS             complement(14682..15674)
                     /locus_tag="PLW16_05510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB70399.1"
                     /translation="MATLHSTARFHRNDIETLGWELTVSNTLDPPESPCRRFLKGQGS
                     YGTHLLAHLERRIPLKRMDRVLEVGGGYGYLMRDLLAVHPFPRVTMLDISPLLLKRQK
                     EALRAYPSVRYVEADFLAIDARRLREFDWIILNENLGDFPTVVGLEGNVREDPDVGAG
                     GEGGSVPARVRRLLDAYSLTTPDRTPFNFNLGAVEALEKICRVGVPYVFLAEHSCEAA
                     VPEPYRRWIDVRAGGNPERIALKGHDEYTIRFSNLVAVAGHYGYEVLRGPVADFLQLD
                     FSPELESILRARNVAVGERETVRHFVEDLFQYEYLLLRKRGVEFRVEAPLKSEG"
     gene            15885..16337
                     /locus_tag="PLW16_05515"
     CDS             15885..16337
                     /locus_tag="PLW16_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB70400.1"
                     /translation="MIKIKFVDSIGNCDREFIEDMEEMFRVSHPFATLRQRLWKPQVD
                     VCETADEVIVLAEVAGVALENLQVEVSRLRFKIHGRREHGQPAREGRYHIAEIAFGAF
                     ERSFNLPTPVDTETVKARYTDGLLTIRMKKAPHDTLHKIRIQNGGSHA"
     gene            16330..18678
                     /gene="lon"
                     /locus_tag="PLW16_05520"
     CDS             16330..18678
                     /gene="lon"
                     /locus_tag="PLW16_05520"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB70401.1"
                     /translation="MPEDTQTEDQKTLTMPESLPILPLFNMVVFPKMMFPMEVAGEQS
                     LSLVDEAMAKDRMIGLTLSKKGPMEASQTPDDYHGIGTSVVILKMAKAADKKAQLLVQ
                     GLGRFRIKSFVEGKPYLQAAIEPLEDREVKDLEVEALMANLVNLFDRIVKLSPFLPQE
                     FGMMAKSITDPGILSDIIASIINASPEEKQRILEILDVKKRLKEVTRLVNHQLEILEL
                     GNKIQTQVKDDMDKSQREYYLRQQLKAIQQELGEADDNRVEIDEYRKKVEEKDLPEEA
                     KKEAERELNRLARMHPSSAEYSVTSTYLDWVTSLPWHDVTEDTLDVRKARKVLDEDHY
                     GLEKAKKRIIEYLSVRKLKPDTKGPILCFAGPPGTGKTSLGHSIARALGRKFVRIALG
                     GVRDEAEIRGHRRTYVGALPGRIVQGIRRAGSMNPVFMLDEIDKLGSDFRGDPSSALL
                     EVLDPEQNFSFTDHYLDVPFDLSHVMFITTANILDTIPPPLRDRMEVIELVGYTLDEK
                     LKIAQRYLVPRQLEANGLTPERLRFTKSALKAIINGYTREAGLRNLEREIAAVCRGFA
                     SLVAADEADRAVIKVKDLHRYLGPVRIPAETGLRTSKPGVVTGMAWTPTGGDLLFIEA
                     TAMKGKKGLTLTGQLGDVMKESATAALSFVRANAVELGIAPDFFEEMDLHIHVPSGAI
                     PKDGPSAGVTILTALTSLLTNRPVKKDLAMTGEITLRGLVLPVGGIKEKVLAAHRAGI
                     RTILLPRWNKKDVEDIPPKVRREVTFHFVDEMLDVLKLALDK"
     gene            complement(18812..20653)
                     /locus_tag="PLW16_05525"
     CDS             complement(18812..20653)
                     /locus_tag="PLW16_05525"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB70402.1"
                     /translation="MNRDFLLDPVHRRRALYVLVACLAVLAAAVFLASQAQRDFGRVR
                     VTDAYYANAGGIPIRAKLFKPLSAGREYPAPGVVYIHGYQNNRETGDAYAIEIARRGF
                     VVLNIDAIGRGHSGIPGEPATADFDRTYGGQASVAYLRSLPFVDPGRVGVMGHSLGAE
                     MAYLVALRDSTLKALVLTGYAYTLEATPTHPKNMLMIIGKYDEFRQRMTATRDIEREW
                     METERTRRAFAIDRPDLGKTYGDFAAGTARRVFVPPITHVHESHHPGAIAETLLWLRA
                     ALAPPPADWIDARRQIWPLKEFSTLVAMLSGFALLLPLGFLLLGTRFFRSLLARPTGG
                     YACTPGDYRKFVTINGVLMWLYLPLILVLFGVHKYLVRIDGAFPMMMVNAIVWWFLWV
                     NIIGFFLFRRWFARQAAPRGVSLLDLGISDQAGTLRVNAGRLVRTALLAVGLFFCMYF
                     LEYLAEKIFLVNYRFVWPFFNDLTPYRWRMFLLYLPWILVCFLQTGAFLHGQLRRPPR
                     TTPMRTFFGRSWENVVALCLPVVLFLAVQYIPLFTTGFIPFEGPNGLFVVFVINLVHV
                     LALLAITAVISTWFFEWTGRIYLGAILNALLVAWSFAASQVIAPIPM"
     gene            complement(20717..21994)
                     /gene="larA"
                     /locus_tag="PLW16_05530"
     CDS             complement(20717..21994)
                     /gene="larA"
                     /locus_tag="PLW16_05530"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPB70403.1"
                     /translation="MRVRLAYDRDGLTVDFPDDGVDVIAPRFVAGLPDEAAALRSALR
                     APLGARPLREVVAPGDDVVIVFPDGTRPMPSARLLPVLLAELDALPPERITLLNALGA
                     HRANTRAELTDMLGAEIVSRYRILQHDPADRDALVDLGRTRFGREIWINRAFVEARVR
                     ILTGFIEPHFFAGFSGGPKAVMPGICGLDTILGNHGAAMIDHPGATWGETEANPVHQE
                     MAEVAERAAPEFLVNVTLNRRREITGVFAGDWRAAHRAGCVFVRQSAMRSVNGPYDCV
                     VTTNGGFPLDANLYQAVKGLSAATRIVRPGGTLVLAAACTEGMQGHENFCRLLYASAS
                     PEALLAEIRGRREPQRDQWQAQILAEVLRRATVHLHSGLPEEAVRRCHMRPVEDVAVF
                     VRAWRDRNPRERIAVLPEGPQTIPYVQPDSGLT"
     gene            complement(22004..22651)
                     /locus_tag="PLW16_05535"
     CDS             complement(22004..22651)
                     /locus_tag="PLW16_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB70404.1"
                     /translation="MFEALSIKDREELAQLLRYQTIKKGEILFRKGDEGTALYLIKSG
                     RIRIVLKVRLEDEMTLAVLSDGDFFGEMSLLDGLPRSADASAHEDTELYVLNRTDFIR
                     FLMHSEHAMKAILYTLSTRLRKTDDLLGETAFLNISSRLAKRLVELAETRGIHQAGSM
                     HVELTLTQKELASLVGVSRESINKELKRWRARNLVATSRNRLIITDLAAIRKHVR"
     gene            complement(22821..24053)
                     /locus_tag="PLW16_05540"
     CDS             complement(22821..24053)
                     /locus_tag="PLW16_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB70405.1"
                     /translation="MMKALLFSASAAKFVVLKPLGALFPSLFYKGPIATVRLAEIPEP
                     ALPGPDWVKLRTLLCGFCGSDQSLVFLKDSPTASPFTSFPCVLGHELCGEVVEAGAGV
                     AGVNVGDRVTLSPGLPCAARGIDPACPSCRAGRPGSCENTARGRLSPGMFTGICRDVN
                     GGFAPYLVAHAGQIYRLPEGMSVEAGALMEPLGVTLQAVLDNRPEPRDRVLVIGAGVI
                     GSLVVQSIRALGIPCTITVAEPSRFHAELAAKAGADHVVTDGDIPGAAVRIAGAASYK
                     PLLGKPILMGGFTKIFDCVGHTDTLRPAMRSLAGGGTLSVVGIGDEVKLDLTPLWLKL
                     QTIRGVYAHGVNSVDGRREHVFETALRFAAEGRVNLKGMITHTFPLERYADMIEVNMR
                     KAANRAVKTAVSFPAESP"
     gene            complement(24235..25905)
                     /locus_tag="PLW16_05545"
     CDS             complement(24235..25905)
                     /locus_tag="PLW16_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPB70406.1"
                     /translation="MGMIYGGHLLAKYLRDVEGVKTVFSLSGGHIDRIYDGFLEYGVR
                     IIDVRHEQAAAMMAHAWSIYGPHAGVCLVTAGPGYTNALTGLVNASLDNVPIVVISGM
                     APRRDWDRGALQGMNQAAMVQTLVKWSGVCHDIRRIPEYVAKAFRHAVAGRPGPVLLE
                     IPPDVLNVKVEEAEVPVPRSGGRVYRSAADDEAVREAAGLIDAARKPFILGGSGVGFS
                     DCEAALAAFVEKTGIPFLLMDNGRGALPDDHPLSLWDGGQMALMTALSTADLIVVFGI
                     RYNWLLMFGQIFPQAKVVRVDIDATEIDRNRASDVGLVGDLGFVLAQLNGAVAKREHS
                     AWLKTLKDMYLPMVAAEVELRGKASDPIHPVRLVERIRETAPDDAIFIADGGDTCYFA
                     LMGLTAKKKAGVLVGAGGLFGCLGTGIPFGIGAKLARPEKTVVVVNGDGSFGLNAMEF
                     DTAVRHGIPFVCVICNDQAWGMIKHGQEMTYGNDRCVGSGLGVVRYDRVVEALGGHGE
                     FVTKDDEIVPALERAFASGKPACVNVITDPCVTSPATLMFVDALTMEK"
BASE COUNT         5125 a         8370 c         8226 g         4683 t
ORIGIN      
        1 ctttaaaatc acttcacttc aggaaatagc agggaatctc ctcaagcgtt acgcttacgg
       61 aaacagggtt gccaacaaaa aatagattcc ggaggataag acgcctgaga caggaatcgt
      121 taaaagccag gcccagacga tgttaccggc cacgccccag cgaacggcgg acaaacgccg
      181 cgtcgaaccg acccccatga tggcgccggt gatcgtatgc gtcgtactga cggggatgcc
      241 ggcaaaagac gcgccgataa tggacagcgc cgccgccgtt tccgcgcaga agcctcccat
      301 gggctgcagc ttggtaatct tggttcccat cgtcttcacg atccgccacc ctccggacat
      361 ggttcccaag gcaagcatgg tgtagcacga gaccaccacc cagaagggga tatgaaaggt
      421 cggtcccaga taacccccgc tgtaaagaac cagggcaatg atacccatgg ttttctgcgc
      481 atcattcatt ccgtggctga tgctgtagat cgccgcggag aacagctgca attttttgaa
      541 aatgcggttc gaccggctga cgttgctgtg acgggaaagg ttcagaacga ccaccatcag
      601 gaatagtccc aggatcaggc cgatcagggg agagatgatg ataaaactgg cggttttgat
      661 aatcccctgc cagatgagga cattgaatcc ccccctcata aggcccgcgc cgatgaggcc
      721 cccgatcagg gcatgcgagg aactgctcgg caaaccgaac caccaggtga tcagattcca
      781 gataatggcg ccgcacaatg cggaaaggat gagcgtatgg ttgacaatgg aagtattgac
      841 gatgcccgtt cccacggtct ttgcgacctg cgcgtcaaac aagaagatcg ccgcgaaatt
      901 gaagaaggcc gcccatacaa ccgcctgata cggtttcagc accttggtcg agacgatggt
      961 ggcgatggaa ttggcggaat cgtggaagcc attgatgaaa tcgaagatca atgccaggac
     1021 gatcacaaag ataacgatgg acagcgatgc gctcattgta ggaaccgatt ttatccttcg
     1081 gcgttgcggt cgttctcttc cggaggcaga tccttcagga tgctccaggc gttgaagatc
     1141 ccgaaacaga ctccgaccag aacgagaaaa atcaggaaaa aggacagact ttggtagacc
     1201 tccagatgcg tctttgaaag cgtttcgacc gcggtcaacc gtttgtccgc ctgcatctca
     1261 atcttggcaa cgatcccgtc aatttgatcc gtgggttcac gatctattcc ccgcaccctt
     1321 tggtcaacga gcaccgcgct tttgaggtcg gcccggttgt agtctttcag ggcatcccgg
     1381 tactgaagac tcagtttttc gtgaaccgtg attgcctccg aaatctcggg gacgacaagc
     1441 cccagggtgg cggtcatgct ggaaagggat tgcagttcct ccttgacacg tcggtcctcc
     1501 tcatcaaagg cccgcaaatg gccttcatag aggtgctgat cgttgccgcg cagaaggata
     1561 tttttccatt cctggacctg ccttttgaag tgaacctgtg cctggcgcgc cgtatcgacg
     1621 gtcttgatca tcatttgccc ttcagaaaca ctttgatgaa ggcgcttgtt ggtttccaca
     1681 acggctatca gaccgaacaa cccgatcacc acggtcagga cggtgaagat cccgatggtg
     1741 atgatgaccc gccgccgaac ggaaatgtcc tggaaaatcg tattgggttc tttcataagt
     1801 ctaagacctc gcttgcggat gtcggtatgt atcaaacagc atgacctttg tccatcacaa
     1861 tgaggatttt cggaatttct gtcggaaagg gggctttcgc ggcccaaggc gggattttaa
     1921 tcacgatgat ccgagcggga tgagtccgac ccaggccttt gcacattcag cggcgggtgc
     1981 gcgggagggg gaccgggtaa cggactccgg ttgatttatt ccgcccgatc ctgtatgcac
     2041 ggcatgcgag gactgatttt agaccctgaa tcccatggtc ttcgccaaag gaagcgcacg
     2101 gggaattcga cgggaaccga acccctggaa atgccgctgc acaggcagta aaacgacggg
     2161 gacaagcatt ccccggggtc gccggacgag aggatcggac caccttgtta atggtcctcg
     2221 gggaaagtat cgagcacgtg aatgagcgtc acacttttat aatgggagag aggccatgcc
     2281 tgactactgg atcaagatca gcgagacgga agaagaggat cgtaagcaac atcattacct
     2341 gacgaccgcc aaggacgaga aagaggcaag aaaagccgcc agggatttta tgaagcgatt
     2401 cattgacgac gatgatgatc cggaagaaat cgaaaacggg tatgcattct acaacaaggc
     2461 catttttgtg cggctggaat ccctcaagga aacgacaaag gagcagttca aggaatttct
     2521 gctgaagatc cacaccatca atatggcgta acttaaagac aaagcctctg taatgcggac
     2581 caatctcagg tttggcggcg attggcggcg gggaagggcc taaggcgggg gcggcttgat
     2641 ccggttcgcg ccggatcttc cggttcagcg atcggctttt aaacgcgcct tcatgcgggc
     2701 caacgcgtcc ctgtccagat gatagttgcc gttccccgtc ctgacgacga ttttatcgcc
     2761 gagcagcacg ttgagggcct tgggtcggat attccccgtg cccacgcgga agatattctt
     2821 tcgctcgtcc tgaatgggaa tcgccctggc cgcactgtag gccccggcct cctccaactc
     2881 ggcaatgatc ttgaagcaca cccgccgcac actcaggccg cccaggtaga ggaccagaac
     2941 gacaaaagcc acgaatgcgg ccagcagcag aaggaacttt aaataaaccg gcatgcgtcc
     3001 tgactccccc tttgaagatt cggcgttccc agcctgcgcc acgggacatg catcccctgc
     3061 gagcacccgc cgatggtatg tcatgggaaa tgccgttgca agcggtttct gttccggaag
     3121 gtccttccca ggtcacctcg cccttgccgg acaatcccgt ctccccgatt tcgcgaggtc
     3181 ctttcttcgt catggatttc tcgtgacaac atctggtaag gagcgggttc agacccggcg
     3241 acgggagggt cggaaaggag cgtgccatga ctttcatcca ggcaatcgaa cggcggatct
     3301 cctgccggac atatcgccag ctcccccttg agcccgaaat gatcgaaggg ctcgcccaag
     3361 cactgctctc gaacacgaag ggaccgttcg gcagccgcgt cagatttgca ctcatccgtt
     3421 cggaccggct ggaccagatc gatctcaagc ccccgggaac ctacggcgtc atcaaagggg
     3481 cccggcagtt catcgcgggt gcggttctgg acaaccccag ggccatggaa gattatgggt
     3541 attgcatgga aaagaccatc ctggcggcga cggccatggg gctcggcacg tgctggctgg
     3601 gagggacctt ccaccggacc ggattcgccg ggcgtatgaa tcttgcggag ggggagctcc
     3661 tgccggcggt ttcacccgtc ggatacgcgt ccgaccaaag atcccttcgc gagcgggtct
     3721 tcaggttcgg cgccggatcg gacaggagaa agccctggga ggacctgttt tacgagagag
     3781 gcatcgtcac cccgctcacg aaagattcgg cgggtcgcta tgcgactccg ctcgaatgcg
     3841 tccggatcgc cccgtcggcg gccaacaagc agccctggag gatcatccgg gaccgcggcg
     3901 cctatcatct ctacctcaag cggacaccgg gatatgaccg gatcttcgcc acaatccgga
     3961 tacagaacat cgacatggga atcgcgatgt gccatttcga gctgtccgca agagagacag
     4021 ggctggacgg cggctggacg gtggaagctc cggggatccc aagcggggat ctcgaatacc
     4081 gggtgagctg gatcgcaaca tcgtagaggg cgcctgcccc ccaatcgtcg tatcgacgta
     4141 ggccgtcatc cgggaaccgt ccgtcacctt cccgtgaatt cggccttgcg cctttcgaaa
     4201 aaggccaacg tgccctcctt caggtcttca ctgttgaggg cttcctggat caggcgctcg
     4261 atctcggcct ggtctcccgg atgaatcccc tgggcgtgaa gcagcttgtt gaaaatatgc
     4321 ttgtgccctt tcagggccag cggggcattg ccggcgatgt cctccgccag acgatccgta
     4381 aacgcgggta gatcggcttt cggcaccaca taatggacca tgcccatcgc ctgcgcccgg
     4441 tggatgtcgt aatagcgccc cgtgaaaaag gcctctttgg cgttggccag ccccagaacg
     4501 ttgatgaatt tctgcacccc cgccggatgg tagatcaccc ccagccgcga gggtggaatg
     4561 cccatccggg aggtgtcgga ggccacccgg agatcgcagg tgatcgccag ctcgcagccg
     4621 gcccccaggg cgaagccgtc gatcatggca atgaccggat agggataggc ctcgatcgcc
     4681 gcaaagccga cttcgagggg gttcttcttt ttcagcgcgt cgaagagatc cggggaaaga
     4741 tcggtcggga tggccgacag gtcgtagccc gcggagaagg ccttgtcgcc ggagccgcgg
     4801 atgacgacgg tccgaattcc tgcgtcccgg gacaactgct cgaactggtc cgccagcgcg
     4861 aacagcacga gcggggtcag ggagttcttt ttgtcgggcc ggttgatgat cagggtcgcc
     4921 acatggcctt tcttctccag aagcaacggt tgttcgtcca cccaaaattc tccttcctga
     4981 ctgtcgattc agaggaccga cccgccctgt ttgaaatagc ggacgtagcc cggaccgcac
     5041 gtgtatcggg aacgatattc cacgagcgac ttgtaccggc cgtagagttc ggggcggtcg
     5101 gacaggtgcg cgatgcgttt cagggccgcc tccgagagct tttcggacac gtgcgcgatg
     5161 tcgccctgcc cctgcagggc atgatggaca atccgccccc agtccacgag gacctcccgc
     5221 gccgcccgca gcgacgtttc ggcgttcggg acgggtccaa agtgggcata gaggacctgc
     5281 tgcggattca gggccgcgag tttctcgatg cttccaacgg cccgaaccag gtcgaactcc
     5341 ggcggggggg tgctggggaa atggacttcc gcctccggaa aatagacccc gagggcttcc
     5401 ccggccagca gggcgccgct cttctcctcg aacaggctca agtgatgcac cgcgtgcccg
     5461 ggcgtgtgaa caacccggag ccgcaccccc cgtcccaggt caatgacgtc tccgtcggcg
     5521 agcggcgcga gctgcgcttc gggaaccggc tccaccgcgc cgtaccaccg ctcggcgttc
     5581 tccccgaaga cccgcctcat gctctcgacg agtttggacg ggttggcgag atggggcacg
     5641 ccccgctcat gcgccagggc ccggaggcgg gggtaccgtc ggcagaggct gcccgcggcg
     5701 ccggcgtggt cgacatggat atggctgaag acgaggcagt cgatctcctc cggctcatag
     5761 ccgatttccc ggacggcatc gaggacgtgc tcaatgaccg tggtcggacc ggtgtcgaag
     5821 agggccaccc ggtcgcccac caggaggtag gtgctggcga acttggcatg gtccagccac
     5881 cgctgatcga tggcgaacag atgtgcatgc aatttctcga tcgtccccat cacgcctccg
     5941 ctcgaacgtt ttgaacgacg cccttttacc gtcattccgg tggcggcgtc aataagcgtt
     6001 tgacgcaccg gccgatcgat cccggggatc ctccctccgg acaccgccct caggaaagca
     6061 gcgccatcca ggcggccatg tcctcttccg cctccaggtc gagaacccgc gccacgatcc
     6121 gctccaggcg gtccggtgcg aaggccaatc cccccagggc gaggaatttt ccgacgatct
     6181 tgtcttcctt ttcccgcggg tccatcacgt gctcgagcat gttgaacgtc tgtctcaggc
     6241 ttcggccctc caggtcgagg cggatctccg cttcgaaggg ggcgagcgta tcgtcgggaa
     6301 ccaggcggat ccgctgttgc agggcgacgg tttccggcgc gtggacccgc tcttccgtga
     6361 aggcgttcat cccggtgtcc cggttcagga gggcgttggc cacgctgtag ggaatgctga
     6421 acttggcctc gaggccgctc tccggccttt ccttgcccgc gacgtccagc atgggccgcg
     6481 aaacgtgaat gtccatcgcc cggatctgcc gggcctccat tccttcgacc cttttcagcg
     6541 ccagggccgc ctcgatcggg gcgtgcgtcc cgtagcagga cgcgtgaaac ttgtagttgt
     6601 tcctgagaat gtaccattga acgcccagtc cctgcaaggc atcctccgac ttccagtcct
     6661 tcgagaacat ctcccagaat cccttctgcc cctccaggat atcccccgtg ctggtgaacc
     6721 cgcgctgggc gaggagggcc gcgagcaggc cgtcgaagga ggccttcccg gcatgaaagg
     6781 gtttgcagga ggttccgaag acggccttga gtccggaggc ctgcgtcccc gccgtcccca
     6841 gggcgtgaat cagttgatcc gcgtccaggc ccaggagttt cgccgttcca gcggccgccg
     6901 cgaaatggcc gatggtggac gtgccgtgcc agccggccag gtaatggttg ggggaggccg
     6961 cgagggcgac gcggcagccg gtttcgtagc ccaggacgta ggcggcaaga aaggcgcgcc
     7021 cgctttcgcg ccgccattcc gcggccgcga ggattccagg gaaaagcgtg acgctgggat
     7081 gccccagaaa ttccacgctg gtgtcgtcga aatccaggac gtgggaactc atgccgttca
     7141 acagggcgac attgagaatg gaagtcttca ccgggcggcc gatcaccgag gcctggggtt
     7201 cgcctccgac cagcgccacc acctgggcga gactgtcgat gtccgcctcc cgggcgccag
     7261 ccagcgtcac ggcaagccag tccagaaagc agagtttggc ctcccggacg acctccgcgg
     7321 gcagatcctg gaattgcgtg cccgcaacat acgccgcgag tttccgggtc gcttccatgt
     7381 caacctcctt aaggacgctg acgtcctttg acgtactgtt cgatcagctt atacttgtag
     7441 attttccccg tcgcggtttg ggggatgggc ttgtccgtga cgatcacctc cttgggaagc
     7501 ttgtacttcg cgaccttccc cacgccgaat tcgaggactt ccccggccgt cagggactgc
     7561 cccggatagg gattcacgat ggccagcccg cactcccccc atttctcgtc cggcatcgga
     7621 atgatggcga tcgccatgat cttggggtgg tcgatgagga tgttctccac ttccttcggg
     7681 tagacgttct cgccgccgct gatgtacatg tccgtcttcc ggtccacaac gaagatatag
     7741 ccgtcctcgt ccaccgtcgc catgtcgccg taatggaacc agccctccgg atcgatggtc
     7801 tcccgggtct tctccggatc gttgagatag cccagcatgg tcatcggccc ccggtagcag
     7861 atctcgccga tttcccccga ccccggccga accggctccc ccgtttttcc cgcgaccttg
     7921 acctcgccgt ggaagagcgg caacccgatg gagcccacct tttccaggtc cttcggtcga
     7981 tccggcgtca gggtgatgca cacggaggtt tccgtggctc cgaagacctg gcccacggct
     8041 tccactttga accggtcgcg gatctgttcg agaaccgatt tgggggtgat ctgcgcgccg
     8101 agggcaacgc ccttcaggga actcaaatcg tagcggtcga agtcggggtg cgcgatgatc
     8161 atctggaaca tggccgggac gccgcccagg aggctgaccc ggtatcgctc cacatcccgg
     8221 aggaccatgc cgggatcgaa atggcgatgg atgagcaggg tccccccttt atggatgacc
     8281 ggcatcgtcg cgataaacag ccccccggaa tggaacatgg gcaggttgag aatataaatg
     8341 acgctggaga cgatataatc aaaggcgttt tcgttgttga gcgtgttcca gagggttttc
     8401 cggtgtggca gaatggcccc cttggcccgg cccgtcgttc ccgaggtaaa caggatgacc
     8461 tgaggatcct ccatcgtcgg aggatcggca accggcggtt cctcgacggg gaatcgccgg
     8521 atccaggatt cgtagtctcc atcggtcgtg tcctgtggcg gatcgatgca gatataattc
     8581 cggatgcttc ccgtccccgg ccaggacttc agctccttgg ccatccccga gaattccccc
     8641 ccgtaaaaga gggcttcggg ggaggactgg gtgatgaaat actcgagttc ccggcttacc
     8701 agacgggtgt tcagggggac gaagacaacg ccggtcttgg cgcaggccca caacagttcc
     8761 aggtacgggt tgcagttgtt cgccaggatg gcgatacggg ctcccttgac caagccgagt
     8821 tccaaaagca tccgcgccgc ctgattgacg cgctcgttca gctcgcggta ggtccattcg
     8881 cgttcctcga agatgacggc caccttgtcc ggggtaagcc tgtgcctttt ggtgacccaa
     8941 tcgccgacgc cggcctcctt catggctcct cctttcggta acacggggtt gaatgaagat
     9001 tacgacctgt ccttcgatgc gacggcatcg cgaatgaaag ccgcgatctc gtcgaagccg
     9061 gcgccctggg tgaaaacgcc ggcaaccccc atcgccttga gatgggggat gtcctcctcc
     9121 ggaatgaacc cgcccatcat caaccgcttg tcggtgatgc cttcctgttc catcctcgca
     9181 atgagttttg tcgcaatgga gaggtgcgca ccggacagga tgctgatccc gatgacgtcg
     9241 acatcttcct ggacggccgt cgtgacgatc tgagtgacgg ttcgccggag ccccgtgtag
     9301 atcacctcca tgccggcgtt ccgcagtctc gaagccagca ggagcgcccc cttgtcgtgc
     9361 ccgtccagac ccgccttgcc caccagaacc cgaatgaccc gatgattcat gaccttcttc
     9421 cctcccccgg agatatgacg gttcaacggg cctcgccgaa gaccccgcgc agggtgtcgc
     9481 agatctcccc caccgttgca taggctttga ccgcgtcgat gaagtggggg atcagattcg
     9541 ctccctcatc gcgggcggca ttctctatct tctccagaca ggactgcacg gcgcgcgggt
     9601 cgcgttcctt cttgacgcgg cggagaccgg cgatctgctt ttcttccgcc tgctcccgcc
     9661 gggcgaccgg ataaggcgag gtgcgcggcg tcatcacgtc gatctcgtcg gcttcggtgt
     9721 atttgttgac cccgaccacc gtggcctccc ccgtctccag gtgttgctga aagtcctccg
     9781 cgctccgcag cacttcattc ttcatccagc cgctctcgac cgccttctcg gcgcctccct
     9841 gtcgatcgat gtcgtcgatc aggccgaaga tctcgtcttc atagcggtcg gtcagggctt
     9901 caacgtagta ggagccggca aagacatccg gcgtatcgcc gagccggcac tcctccatga
     9961 tgatccgcgc ggcgtccgcc gccagctggc gggcctcgac gctgtggccg agtccgagcg
    10021 cctcgtcgta ggggggctcg caggccggcg gctcgccgat catcgccgcg aaggcggcgc
    10081 cgatggcaac ccggacgagg ttgttcaggg gccgctggcg ggtgcaggtc cagaaaccgg
    10141 ccaaaccgcc gccgagctcc cggtagatcc agttggacgg cttgacggcg cccagccggt
    10201 gccgcatgat cttggcccag acccgtcgcg ccgcgcgccg gcacgccgct tctttcagaa
    10261 actccatgcc gccgccgaaa ttgaggaagg tcgccttcgc ggcaacctcg tcgacgctca
    10321 tgcccgcgtt caccgcgacc cggaagtagg ccatggcgtt ggccatggca aaggcgacgg
    10381 cctgcacccg ggtggcgccg gcctcgcgga tatgatagcc gcagatgctg atggtgttca
    10441 tgttgggcag gtgctcatgg cagtacgtga aggtgtcgcg ggtcatgcgc agggaaggac
    10501 cgacgggaaa gatctgcgtg ccccgggcga caaactcctt cagaatgtca ttctgggggg
    10561 ttccccgcag cttgtcgagc gggatgccgc gcttttgggc cagggcgacg tacatggcca
    10621 ggatgacgtt ggtcgtcgcg ttgatcgtcc agttggaggc gatccggtcg aggtccgtgt
    10681 cgccggtgaa ggcctcgtaa atcgtctcga agtccctaag ggtatcgatg gccaccccga
    10741 cccggccgac ttcaccctcg gccagttcgt gatcggaatc gtagccggtc tgggtcggca
    10801 ggtcgaaggc gatgttcgga ccgcccccgc gcgccatcgt cctgccgcgc tcgatgaaga
    10861 aatcccgcat gtcctcggcc gtcccgtaac cggcataccc gaaggcgtgg cggatgcttt
    10921 tgccgccgcc cagggttttg ccgcggcccc agatgacctg gtgcagggga acggcaaacc
    10981 ggccggccgc aaagggatac tcaccgggga agcccacccg ctcgagaaag tcatcctctc
    11041 cgatatcgag gggactggcg aactccacgg gactggccgc gagacccatc cgctgcagat
    11101 tcttctttaa aacgccctct tcccaagctg tccgggctgc ccggatcctg tccgaggtct
    11161 gctggtccaa cggcatcgtc ttccctcctc aacggcgaat gagtgatgtc cccgagaaac
    11221 tgcattcaaa aaattgcaga caagaccatc ccccctgccc ggcatggcct atccctgttc
    11281 gacggacgtt ttcggccggg cgggatcgcc gatcacggtg gcggcgatcg ttcccagcac
    11341 ggcgacgacg aggaggacct gaacggcgcc ggaataggag ccgctcgcgt cgtacatgcg
    11401 ccccagcaga atcgcccccg tggcgagcga caggatctgg atcaggccaa agacgccgac
    11461 gaacttgctg aaatgatcca gtccgaactt cctgcccacc atcaggggaa acaacgtcgg
    11521 gaacgccccc agggcgcatc cgtagaagat gacaaagccc cagacgagcg gggcggcgtc
    11581 gacgagatac acgacggaaa gcaggccgat gagatggaac ccccagatga cgatcatcag
    11641 gcccttttgg gaaatcctgt cggccgcccc gccgaacgcc agccggccga gcacgctgaa
    11701 tccgagcgcc aggctccaga agaggctggc ctggtcgaca ctcagacttc tcagcagggc
    11761 aatggcgtgc tgggtcatgc cgcccaggat caggctgtag aagatcgtca ggaaggcgag
    11821 gacatggaag ggcaggcatt tgaaggcctg ggcgacggtg aaaccgaagg ctgacgcccg
    11881 ctcctgtgtt tccgtcgccg tcagcggaat gccatccgga tcgagtccga ggtccgcggg
    11941 cttctccttc acgacggcga gaatcaaggg caggacgatg cccagcacca ggagaccgag
    12001 cagacggtac gcgccccgcc agccaaaaga cgccgcggcc tggctcgcga caaagaccat
    12061 aaccatgccg ccgacgccga tgccggacat gatgattccc atggccatgc cgcgcttccg
    12121 gttaaaccag tgagagatca gggtctgtga agggacgatc gataccgagg ccagtcccgt
    12181 ggccatgaag atgagcagcg cgtagtagtg ccaaagttgc tgcatcgaac cgagcatcag
    12241 caaggcaagg ccgacggttg tcgcgccgta gagctggact ttcttgaccc cgaatctggc
    12301 caccgccacg cccacgaccg ggctcaagag ccccaccaga agcgcggcca gggtgaaacc
    12361 gatggaaacc tgtgtcgcac tccatccgaa cgctcgcctc aggggttcca ggaaaacgga
    12421 aacggtatag aatccggaac tcaacccgat cagaagaacg atgaatgtcg ccgcgacgat
    12481 ccaccacccg tagaatacct tgccttccct ggccatagct ccctcctttg ggttaccggc
    12541 gttggtcagc gtttggttga cggttgggtt ttcgcattaa cgttcatgga tgcgacgcgt
    12601 ggcgctgcgt gtcgctccaa aggtcggaca ccaggcggag tgctttcccg aaccgccggc
    12661 gacgatgacg cgcatgtccc cgggagttcg gaccaggggg atgccttctt tccattcctc
    12721 gaggcggttc tcatagcccc cccatgagga cagttccgcc agcttctcag gggtaatcct
    12781 cgccgtggcg acccgggccc gctcgtagag aacctgctgc acgccttcac gcgagaagcc
    12841 cccgtcggcg atggtcctgg cgtgttccgg ccccaggatg agataggtgt ctcccccgac
    12901 gtacaggttg ttgttcccga ccgtggccat ggtctgcgca atcgtgttga gcagggatgc
    12961 cgccgtcgaa gatccgtgat cgttgatgtt gtgcggcgcc tcggcggcga caacggtcac
    13021 acagctctct tcggccttga acccgagcgc ggtccggaac gggagccagg gggttttctc
    13081 ctcgttctcc gcaaaacagt acgtaaattt cgccggagac ccgagggttg ccttgtccaa
    13141 ttctccaggc gttgcgccgc cgacattctg gagaacgaga cgcaccgccc gaccgatcgt
    13201 cgcgttggcg cggttcccgt tccccaggca gttatggccg ctgttcatgc cgatggtccg
    13261 gctgatgggc ccgtggacga tcaccaggat cccgcaattg tgcgtcgttg tctggacgcc
    13321 gtacaggtta aaatcggcct cctgggtcgc ttcgatggcc gcgacgatca ccggcagaaa
    13381 ttcgggccgg catccggcca tgacggcgtt gatggcaata tccagcaggg tggccgccgc
    13441 gtttctcggc aggaggcagc cgatggggtc cagggggttc ccggacacgg atcggagcgc
    13501 ggccttcacc cggtcctcgg tgggaaggac aaccggaagg ccgtccgtcc agccgcgttg
    13561 atgaaattcc tcgagcgcgt ccgcttcacc gccttcgatg aggatccggc ccgcatccgc
    13621 cccggcggat ccatcccctt ccggcgttcc cggggacgag gatgcgcctt ctccagtcag
    13681 ggatgaggcg acgcctcgaa caacggcgtc ggtgatgcgc gtccgggcct ccgtcacgga
    13741 aatgcccccc acgggatggg caacctgcac gaccggcagg gacgcctcgt gccgttcttt
    13801 cagcaggctg cccgccagat cggcgaacat cgcggtgacc atcagaaccg cggggcgcga
    13861 cttccgggag agggcgcaca ggtcgtggac actccacgac gtacacgatc ctcaatcgcc
    13921 aacggcaagg acgacggcat cgcatcgatc gcggatctcc gcgatcagct cgtccggggc
    13981 ggccagcgaa gggatctgct tccggaagtg gagcgcttcg atgggaaatt gctttttgag
    14041 ttccgccgca agcgtttcca gcaatacatc agtgttgggc ttgccattgc cgagaaatcc
    14101 gatccgtttt ccggagagcg cttccaagcg gtggacggga tgagacatcc ccgtttctga
    14161 aacgccaacc ggcatatacg cgtggacaaa accgggcttc gtcatcacgg gcctcctgga
    14221 ttgacggttc gagtgaaaga atttggcttc tctgggaggt cagattccgg aaacgctcat
    14281 accggtctct tcggcgcccc cgctccccac tccatccatc gtcgggtgtg tccgccgggc
    14341 cgggtggtcc cggcgcggcc tcatcggaat tgcgtgcaga tttcagaaga accgggcgcg
    14401 tagtgccggc agacggccgg ccgcgtctcg tagatcgtac aggtataatg gtcgtcgtcc
    14461 cactggagga aggggcatcc gtgcaggtag cggccgtcgt cggaggaaat caggtgatcc
    14521 cccgcccaga cggcatgctc gtgggcgatg atccggagga tgtcgtcccg cccctcccgg
    14581 cgccaccgct cccagtcctc gtccgtcacg tacgcgatca ggttcgccag gcagcatttc
    14641 ccgcatctcc ggcagacgat ctccggttgg ttttccatca ctcacccctc tgatttcagg
    14701 ggagcttcaa cgcgaaattc aacgccccgt ttccggagca gcagatactc gtattgaaac
    14761 aggtcctcga caaaatggcg gaccgtttcc cgctcgccta cggcaacgtt ccgcgcccgc
    14821 aaaatagact ccaattccgg ggaaaaatca agctgtaaaa aatccgccac cggcccacga
    14881 aggacctcat atccgtaatg accggcaacg gcgacgagat tcgagaaacg gatcgtgtat
    14941 tcgtcgtgac ccttgagggc gatgcgttcc ggattccccc ccgccctgac gtcgatccac
    15001 cggcgatagg gctccggcac ggccgcttcg caactgtgct ccgcgagaaa gacataggga
    15061 acgccaactc ggcagatttt ctccagggcc tcgacggccc cgaggttgaa attgaacggc
    15121 gtccggtcgg gggtcgtcag ggaataggcg tcgaggagtc gcctcacccg cgccggcacg
    15181 gagcccccct ctccgccggc accgacgtcc ggatcctccc gaacgttgcc ttccaacccc
    15241 acgaccgtcg ggaaatcccc gaggttctcg ttcaggatga tccagtcgaa ctcgcgcagg
    15301 cgccgcgcgt cgatggcgag gaaatccgcc tcgacgtagc gcacggacgg gtaggcccgg
    15361 agggcctcct tctgccgttt caggagcaac ggggaaatgt cgagcatcgt cacccgggga
    15421 aacgggtgca cggcgaggag atcccgcatc agatagccgt agccgccccc cacctccagg
    15481 acccggtcca tccgtttcag cgggatccgt cgctccaggt gggcgagcag atgcgttcca
    15541 tacgatccct ggcccttcag aaaccggcgg caggggctct ccggcgggtc gagggtgttg
    15601 gagaccgtca gctcccatcc cagggtttcg atgtcgttac ggtggaagcg ggcggtggaa
    15661 tgaagcgttg ccatgtggcc tttccttgtt cggcagagcg ggcggatgcg gcgtcatgct
    15721 gccacgccta catccgcgcg tcaacgggaa acttgcagcg aaaacgtccc cgtggacatc
    15781 cggggcttta caaacctctt ttcggtacta tattacagac tcgtggacag tcgaccgcgg
    15841 atggacgtcg ttcggatcgg tttcgaaccc cagaggaaag cgcgatgatc aagatcaaat
    15901 ttgtggacag catcgggaat tgcgacagag agttcatcga agacatggaa gagatgttcc
    15961 gcgtctccca ccccttcgcc acgctgcgtc aacgactctg gaagccccag gtcgatgtct
    16021 gcgaaaccgc agacgaggtg attgtcctgg ccgaggtggc tggtgtcgcc ctggaaaacc
    16081 tccaggtgga ggtcagccgg ctgcgcttca agatccacgg ccggcgcgaa catggacagc
    16141 ccgcccgcga ggggcgttac cacatcgcgg aaatcgcctt cggggcgttc gagcgaagct
    16201 tcaacctgcc gacgccggtg gacacggaaa ccgtcaaggc gcgctacacg gacggtctcc
    16261 tgaccatccg catgaaaaag gcgccccacg acacgctgca taaaatccgc attcaaaacg
    16321 gaggatccca tgcctgaaga tacccagacg gaagaccaga agaccctgac aatgccggag
    16381 tccctgccca tcctgcccct gtttaacatg gtcgtcttcc cgaagatgat gtttccgatg
    16441 gaggttgcgg gcgaacagtc gctcagcctc gtcgacgagg cgatggccaa ggaccggatg
    16501 atcggcctga ccctttcgaa gaaaggtccg atggaggcct cccaaacgcc ggacgactat
    16561 cacggcatcg gcacgagcgt cgtcatcctg aagatggcca aggccgcgga caagaaggcc
    16621 caactcctcg tgcaggggct cggacggttc cggatcaagt ccttcgtgga ggggaagcct
    16681 tacctccagg cggcgatcga acctctcgag gaccgagagg tcaaggacct cgaggtggag
    16741 gccctgatgg ctaacctcgt caacctcttc gaccggatcg tgaaactttc cccctttctc
    16801 ccgcaggagt tcggcatgat ggccaagtcc atcaccgatc cgggcatcct gtcggacatc
    16861 atcgcgtcga tcatcaacgc ctcgccggag gaaaagcagc ggatcctgga aattctggac
    16921 gtgaagaagc gcctcaagga ggttacccgt ctggtcaacc accagttgga gatcctcgag
    16981 ctgggcaaca agatccagac ccaggtcaag gacgacatgg acaagagcca gcgggaatac
    17041 tatctccgcc agcagctcaa ggcgattcag caggaactcg gggaagcgga cgacaaccgg
    17101 gtggagatcg acgagtaccg gaaaaaggtc gaagagaaag acctcccgga ggaggcgaag
    17161 aaagaggcgg agcgcgagct gaaccgcctg gcgcggatgc acccctcgtc ggcggagtac
    17221 agcgtgacct ccacctacct ggactgggtc acgtccctgc cctggcacga cgtgacggaa
    17281 gacaccttgg acgtccgaaa ggcccggaag gttctggacg aggatcacta cggcctggag
    17341 aaggccaaga agcgtatcat cgaatacctg tccgtgcgga agctcaaacc cgacacgaag
    17401 ggtccgatcc tctgcttcgc cggacctccg gggacgggca agacttccct cggccattcg
    17461 atcgcccgcg ccctgggccg gaaattcgtt cgcatcgccc tgggcggcgt gcgcgacgag
    17521 gcggagatcc ggggacaccg ccggacgtac gtcggcgccc tgccgggccg gatcgttcag
    17581 ggcatccgcc gggcgggatc gatgaatccc gtcttcatgc tggacgaaat cgacaagctg
    17641 ggaagcgatt tccggggcga tccctcatcg gcgctcctgg aggtcctcga cccggaacag
    17701 aatttctcct tcacggacca ttacctggac gtgccgttcg atctgtccca cgtcatgttc
    17761 atcacgacgg cgaacatcct ggacacgatc ccgccgccgc tccgggaccg gatggaggta
    17821 atcgagctcg tcggctacac gctggacgaa aagctcaaga tcgcccagcg ctacctcgtt
    17881 cccaggcagc tggaggccaa cgggctgacg ccggagcggc tcaggttcac gaagagcgcg
    17941 ctcaaggcca tcatcaacgg ctacacgcgc gaggcggggc tccggaacct ggagcgggag
    18001 atcgccgccg tctgccgggg ctttgcaagt ctcgtcgccg cggacgaggc cgaccgcgcc
    18061 gtgatcaagg tcaaggacct ccaccggtac ctcggcccgg tccgcatccc cgccgaaacg
    18121 ggcctccgca cgtccaagcc cggcgtcgtc acgggcatgg cctggacgcc gacgggcggc
    18181 gacctgctct tcatcgaggc gacggccatg aaggggaaga aggggctgac gctgaccggt
    18241 cagctgggag acgtgatgaa ggagtccgcg acggcggccc tgagcttcgt ccgcgccaac
    18301 gccgtcgaac tcgggatcgc gccggacttc ttcgaggaga tggacctgca catccacgtc
    18361 ccctccgggg ccatccccaa ggacggcccc tcggcggggg tcacgatcct gacggccctg
    18421 acctcgctgc tgacgaaccg gccggtgaag aaagacctcg ccatgacggg agagatcact
    18481 ctgcgggggc tcgtgctgcc cgtcggcggg atcaaggaga aggtgctcgc cgcccaccgc
    18541 gcgggcatcc ggacgatcct cctgcccagg tggaacaaga aggacgtgga ggacatcccg
    18601 cctaaggtcc gccgggaggt cacgttccac ttcgtcgacg agatgctgga cgtcctgaag
    18661 ctcgccctgg acaaatagcg ccgctgtccc tgatttccca taccgggacc cgcgggaatc
    18721 atgaccccac cgcgcgctgg accgttttcg atcccgagaa atctcccatg cgcgcgtgaa
    18781 gagatcacga ttcctaacgc gggcaacggt ctcacatggg aattggggcg atgacctgcg
    18841 aggccgcgaa cgaccaggcg acgaggagcg cgttcaggat cgcccccaga tagatccgcc
    18901 ccgtccactc gaagaaccac gtcgagatca ccgccgtgat cgccaaaagc gccaggacgt
    18961 ggaccaggtt gatcacgaac acaacgaaga gcccgttcgg cccctcgaag ggaatgaagc
    19021 ccgtcgtgaa gagcgggatg tactggacgg ccaggaacag gacgaccggc aggcacaggg
    19081 cgacgacgtt ttcccacgac cggccgaaga acgtccgcat cggcgtcgtc cggggcgggc
    19141 ggcggagttg cccgtgcagg aaggccccgg tctgcaggaa acacacgagg atccagggca
    19201 gatagagcag gaacatccgc caccggtagg gcgtcagatc gttgaagaac ggccagacga
    19261 accggtagtt caccaaaaag atcttctccg cgagatactc cagaaaatac atgcagaaga
    19321 acaggccgac ggccagaagg gccgtccgga cgagccggcc ggcgttcacg cgcaaggtcc
    19381 cggcctgatc ggagattccc agatcgagaa ggctgacgcc gcgcggggcg gcctggcggg
    19441 cgaaccagcg ccggaacagg aaaaagccga tgatgttcac ccacaggaac caccagacga
    19501 tcgcgttgac catcatcatc ggaaaggccc cgtcgatccg gaccaggtac ttgtggacgc
    19561 cgaacaggac gaggatgagc ggcaggtaaa gccacatcag cacgccgttg atcgtgacga
    19621 acttccggta atccccgggg gtgcaggcat agccaccggt agggcgcgcc aggagggacc
    19681 ggaagaaccg cgtccccagc agcaggaacc cgaggggcag tagcagggcg aagccggaca
    19741 gcatggcaac gagcgtcgag aactccttga gcggccagat ctgacgccgg gcgtcgatcc
    19801 aatccgccgg cggcggcgcc agggccgccc ggagccaaag cagggtttcg gcgatcgccc
    19861 ccgggtggtg ggattcatgg acgtgggtga tgggcggcac gaagacgcgc ctcgccgtcc
    19921 ccgcggcgaa atcgccgtag gttttcccga gatcaggccg gtcgattgcg aaggcccgcc
    19981 gggtgcgctc cgtctccatc cactcccgct cgatatcccg cgtcgccgtc atacgctggc
    20041 ggaactcgtc gtatttgccg atgatcatga gcatgttctt cgggtgcgtg ggcgtcgcct
    20101 ccagcgtgta ggcgtagccc gtcaggacaa gggccttgag cgtcgaatcc cggagggcca
    20161 cgagataggc catctcggcc ccgagactgt gtcccatgac gcccacccgc ccggggtcca
    20221 cgaacggcag ggagcggaga tacgccaccg aggcctggcc gccgtaggtc cgatcaaaat
    20281 ccgccgtcgc gggctctccg ggaatgcccg agtgcccgcg gccgatggcg tcgatgttga
    20341 gcacgacgaa tcctcgtcgc gcgatctcga tcgcgtaggc gtcacccgtc tcccggttgt
    20401 tctggtagcc gtggatatag acgaccccgg gggcggggta ctcccggccg gccgacaggg
    20461 gcttgaagag cttggcacgg atcggaatgc cgccggcatt ggcatagtac gcgtccgtca
    20521 cccttacccg gccgaaatcc cgctgcgcct gcgaggccag aaagacggcc gccgccagca
    20581 cggcgagaca ggccaccaga acgtaaagcg ccctccggcg atggaccgga tccagaagga
    20641 agtctcgatt catgggaacc tccacgattg tccgggggtc gacgatctca tcaaaaaccg
    20701 aaatccgtct ttcccgtcat gtcaagccgc tgtcgggttg aacgtaggga atcgtctgcg
    20761 gcccctccgg caggacggcg atccgctctc ggggatttcg atcccgccac gcgcgaacga
    20821 acacggccac atcctcaacc ggtctcatat ggcagcggcg caccgcctct tccggcaggc
    20881 cggagtgcag atggaccgtc gcccggcgca ggacctccgc caggatctgc gcttgccact
    20941 gatcgcgctg cggctcccgc ctgccgcgga tctcagccag aagggcctcc ggcgaagccg
    21001 atgcgtacag gagccgacaa aagttttcgt gcccctgcat cccctccgta caggccgccg
    21061 ccaagacgag ggttcctccc ggccggacga tccgggtcgc cgccgacagt cccttgacgg
    21121 cctggtagag gttggcatcc agggggaacc cgccattggt cgtcacgacg caatcgtaag
    21181 gcccgttcac cgaacgcatc gcggactgcc ggacgaacac gcagccggca cggtgagccg
    21241 cccgccagtc gccggcgaaa acgcccgtga tctcccgccg ccggttgagc gtgacgttga
    21301 cgagaaattc cggggcggcc cgctccgcga cttccgccat ttcctgatgg acgggattcg
    21361 cctccgtctc cccccaggtg gcgcccgggt ggtcgatcat cgcggcgcca tggtttccca
    21421 ggatcgtgtc gaggccgcag atcccgggca tcaccgcctt cgggccgccg gagaagccag
    21481 cgaagaaatg gggctcgatg aaccctgtca gaatgcggac ccgggcctcc acaaacgccc
    21541 ggttgatcca gatctcgcgg ccgaagcggg tgcgtccaag atccaccaag gcgtcccggt
    21601 ccgcgggatc gtgctgcagg atgcggtagc ggctcacgat ttcggccccc agcatgtccg
    21661 tcagctccgc gcgggtgttg gcgcggtggg cgcccagggc gttgagcagc gtgatccgct
    21721 cggggggcag ggcgtcgagt tcggcgagaa ggactgggag gaggcgggcg gagggcatcg
    21781 gccgggtccc gtcgggaaag acgatgacca cgtcgtcgcc gggggccacg acctccctca
    21841 acggccgggc gccgaggggc gcacgcaggg cgctccgcaa ggcggccgcc tcgtccggaa
    21901 gccccgccac gaagcgcggc gcgatcacgt cgacgccgtc gtcgggaaaa tcgaccgtca
    21961 gtccgtccct gtcgtacgcc agccgaaccc gcatcgtccc tcctcaccgg acatgtttgc
    22021 gaatagcggc caggtcggtg atgatgaggc gattgcggga agtagcgacg aggtttcttg
    22081 cccgccaccg cttcagttct ttattgatgc tctcccggct gacgccgacc agactggcca
    22141 gttccttctg agtcagcgtg agttcaacgt gcatcgaacc cgcctgatgg atcccccggg
    22201 tctccgccag ttccacaagc cttttggcca ggcgggagga gatgttgaga aaggccgtct
    22261 cgcccagcag gtcgtccgtc ttgcgcaacc gcgtggagag ggtgtagaga atggccttca
    22321 tggcatgttc gctatgcatc aagaaacgga tgaagtcggt gcgattgagg acataaagtt
    22381 ccgtatcttc gtgggctgag gcatccgcgg agcggggcaa accatccaga agggacattt
    22441 cgccgaagaa atccccgtcc gaaaggaccg cgagggtcat ctcgtcctcc agccggacct
    22501 tcaggacgat cctgatccgc ccgcttttaa tcagatacag ggctgttccc tcgtcgccct
    22561 tgcgaaaaag gatctccccc ttctttatcg tctgatatcg caggagctgc gccaattcct
    22621 cccggtcttt gatggataaa gcttcaaaca tgctgatctt cttgaggaga ctgcccgggc
    22681 tcattctttc ctcctgttat cgggtgaacc accttgggaa ggtcgctcgt ccttttcttc
    22741 catggtttgt cgtcggttgt caatccggaa atgacgcctt tcccccgttt ttcatgattc
    22801 aggggaacgg aagtcggact ttacggtgac tccgccggga aggacacggc cgtcttgacc
    22861 gcccggttcg ccgccttgcg catgtttacc tcgatcatgt ccgcgtagcg ctccaggggg
    22921 aaggtgtgag tgatcatccc cttgaggttc acccggcctt cggcggcgaa gcggagggcc
    22981 gtctcgaaga cgtgctcgcg gcggccgtcc acggagttga cgccgtgggc atagacgccc
    23041 cggatcgtct ggagcttgag ccataggggc gtgaggtcca gcttgacctc gtccccgatg
    23101 cccacgacgc tcagggtgcc gcccccggcg agcgaacgca tggccggccg cagcgtgtcg
    23161 gtgtggccga cgcagtcgaa gatcttcgtg aagccgccca tcaggatcgg tttcccgagg
    23221 agcggcttgt aggacgccgc gcccgcgatg cgcacggccg cccccgggat gtcgccgtcc
    23281 gtcaccacgt ggtcggcgcc cgccttcgcc gcgagctcgg cgtgaaaccg ggaaggttcc
    23341 gccaccgtga tcgtgcaggg gatgccgagc gcccggatcg actggacaac caggctgccg
    23401 atgaccccgg cgccgatgac gagcacccgg tcccgcggct ccggccggtt gtcgagcacc
    23461 gcctggaggg tcacgcccag gggctccatg agggcgccgg cctcgacgga catcccttcc
    23521 ggcagccggt agatctgacc ggcgtgcgcg acgagatacg gggcgaagcc gccgttgacg
    23581 tcccggcaga ttcccgtgaa catccccggc gacaggcgtc cccgcgccgt gttctcgcag
    23641 ctcccggggc gtccggcccg gcagcttggg caggccggat cgatcccgcg ggcggcgcag
    23701 ggaagccccg gcgacagcgt cacgcggtcc ccgacgttaa cccccgcgac gcccgccccg
    23761 gcctccacga cctcgccgca cagctcgtgg cccaggacgc aggggaagga ggtgaagggc
    23821 gaggcggtgg gggaatcctt gaggaagacc aggctctggt cggagccgca gaacccgcac
    23881 aggagcgtcc ggagcttgac ccagtccggc cccggcaggg cgggctcggg gatctcggcc
    23941 agccggaccg ttgcgatcgg ccccttgtag aagagagacg gaaacagggc gcccaggggc
    24001 ttgagcacca cgaacttcgc cgcggacgcg gaaaacagca gcgctttcat catccccccc
    24061 cctttttgag cagggtgtcg aaagacaccc ggattgtaca ggccgttcaa aaatgctcag
    24121 gtgcaaggcg cgcaaaaacc gaagagcgag acgtatatgg acatacgtcg agtgatgagg
    24181 tttgcaatgc aacgccgcgg atgagcgttt ttcaacagcc tgttagaaaa gcgatcactt
    24241 ctccatcgtc agggcatcca cgaacatcag cgtcgccggg ctcgtcacgc acgggtcggt
    24301 gatgacgttg acgcaggccg gcttgccgga ggcgaacgca cgctcaagcg ccgggacgat
    24361 ctcatcgtcc ttcgtcacga actcgccgtg accgcccagg gcctcgacca cccggtcgta
    24421 gcggacgacc ccgaggccgg agccgacgca ccggtcgttg ccgtaggtca tctcctgacc
    24481 gtgcttgatc atcccccagg cctggtcgtt gcagatgaca cagacgaagg ggatgccgtg
    24541 gcggaccgcg gtgtcgaact ccatcgcgtt caggccgaac gagccgtccc cgttgacgac
    24601 gacgacggtt ttctccggcc gggcgagctt ggccccgatc ccgaagggaa tccccgtgcc
    24661 caggcagccg aagagcccgc ccgctccgac gagaacgccg gccttcttct tcgccgtgag
    24721 gcccatcagg gcgaaatagc aggtgtcgcc gccgtccgcg atgaagatcg cgtcgtccgg
    24781 ggcggtctcg cggatccgct cgacgaggcg gaccgggtgg atcgggtcgc tggccttacc
    24841 gcggagctcc acctccgccg cgaccatggg caggtacatg tccttgaggg tcttcagcca
    24901 ggcactgtgt tcccgctttg cgaccgcgcc gttcagctgg gccaggacga accccaggtc
    24961 gcccacgagg ccgacgtcgg acgcccggtt gcggtcgatc tccgtcgcgt cgatgtccac
    25021 ccgcaccacc ttggcctgcg ggaagatctg gccgaacatc aggagccagt tgtagcggat
    25081 cccgaaaacg acgatcaggt cggccgtcga cagggcggtc atcagggcca tctggccgcc
    25141 gtcccaaagc gacagggggt ggtcgtccgg gagggcgccc cgaccgttgt ccatcaggag
    25201 gaaggggatg cccgtcttct cgacgaaggc tgcaagcgcc gcctcgcagt cgctgaagcc
    25261 gacgccgctc ccgcccagga tgaagggctt ccgcgccgcg tcgatcagtc cggctgcctc
    25321 ccggaccgcc tcgtcatccg cggcggagcg gtagacgcga ccgccgctcc gcggcacggg
    25381 aacctcggcc tcctcgacct tgacgttcag aacgtcgggg ggaatctcca ggaggaccgg
    25441 cccgggacgg cccgcgacgg cgtggcggaa ggccttggcc acgtactcgg ggatccgccg
    25501 gatgtcgtgg cagacgccag accacttgac gagggtctgg accatcgcgg cctgattcat
    25561 cccctgcagg gcgccgcgat cccagtcgcg gcggggggcc atgccgctga tcacgacgat
    25621 cggaacgttg tcgaggctcg cgttgacgag gcccgtcagg gcgttggtgt acccggggcc
    25681 ggccgtcacc aggcagacgc cggcgtgcgg tccgtagatc gaccaggcgt gggccatcat
    25741 cgccgccgcc tgttcgtggc ggacgtcgat gatccggacg ccgtactcga gaaatccgtc
    25801 gtagatccgg tcgatgtggc ctccagagag cgaaaagacc gtcttcaccc cctcgacgtc
    25861 cctcagatac ttcgccaaca gatgaccgcc gtagatcatg cccataacgc cctcctaggt
    25921 gtggattccg tgtgaccggc ccgttcgggc cttccgtgat gctttgatga attggaagat
    25981 ggacgcgttt cccccacaaa tggggcccca gtcggagacc gtcaggaaac agtgattcct
    26041 ctatagggga acgcccctct tttttcaatc aaaattgacg acaaaatgcg gcatgtccgt
    26101 catggcggat gggccaaact ttgacccgag acctgtcact gcgtgcaatt aattgcatgc
    26161 attccccggc cggcccgggg cggcggaagg aacgtttacg gcgagcgccc cgacggagcg
    26221 cggtcgcggg caaaaaaaaa cgcttgacag gcccgtccgg atattatacc aaccggttag
    26281 tttaaacttt tggttgaaaa aggccgaatc cccagacacg gcacccggcc cgaaagcccg
    26341 cgtcgtttcc cttcggcccc ttgaacccag gtcgccatga tggatcccgt atccgcacag
    26401 aacc
//
LOCUS       DAOOLJ010000022        24769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_1890_length_24769_cov_18.224326, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000022 DAOOLJ010000000
VERSION     DAOOLJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 24769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24769
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1890_length_24769_cov_18.224326"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1316)
                     /locus_tag="PLW16_05550"
     CDS             complement(129..1316)
                     /locus_tag="PLW16_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70407.1"
                     /translation="MIRINSYLHREELLEIIRRWMYSDVHPADAGRVTRLVHFNQEYI
                     ARYLREFAVRLFRGLFGMDPVFQPVTVKGELKDCLVARPPYRNERIDRLIRDYAAFPG
                     HYFRETPFDGWLVFVGREDPAALAGSMRIKRDRRLAEKAARRITDRIFDRIRAHADQL
                     AEARARRLGIQPGQLVTTPEDMRDEFLRAEARLLSNLRHRQAIEDGEPLAINDVAGLK
                     LILPGERLGELESVLASLDGCSVLEKEQHTGHYNAVNYIVRLEPPREALLAEPLSAGV
                     VEILKRKGDDPEATVREFAAFVRAGEPDVHLEIIVSDYQETLESEVGRCMHEDRIIEQ
                     RLRREYRGSMVKNMEYLLEYLFAFATSPRRELETLPVKLWDRYLPDYFDGVLKELYGI
                     PDS"
     gene            1518..2045
                     /locus_tag="PLW16_05555"
     CDS             1518..2045
                     /locus_tag="PLW16_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70408.1"
                     /translation="MNDYREAPAGRKSLKRAYVAVMLWFVGRALQVAARADAEVRREF
                     EALPEGFTFAMGVLPAGPWMIVGKRAGRIRYLGWRPEGIALDLTLKIKHLEAAILMFT
                     FRESTAVATARNRMIVDGEVPPACAIVRILDIVEVYLLPKFIARLAVKRYPRWSWTRK
                     LVGRTRIYLGAVLGR"
     gene            2058..4127
                     /locus_tag="PLW16_05560"
     CDS             2058..4127
                     /locus_tag="PLW16_05560"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB70409.1"
                     /translation="MEIPRHFEFFCPIRIFSGSRALEHLPGELRALNASRPLVLATQG
                     TADRGLTRILVKAFADSGMPLGLHEAAPESPDAAWTARVKHLFVEHRHDALLVMGDGP
                     LVTAAKILNIAVSIGPEDDLAAASGDNRLTRPLLPFVVIPTGVDCEAVASPTAALGPW
                     TFRSPALMAALVALDPRTIRPGDAGRDAAVLSVARAHAARVIAAAPRNPVGEAYARAG
                     LALLDRNGSKAGKGERWALANGVLLAGCAHAQAEPAGPDAAEAAFTGEPATAEAASAY
                     LPDYYEFLNGGRIVAGHDALKRIPTILADLGAKNPLIVTDRGVAGAGLVEVARNALRD
                     ALALETIADDVPVDSDIKAVRRIAELYRQRHCDSFIAVGGGSVMDTAKGVNIVVSENA
                     RDLMAYSGVGAIRSRLRPLIAVPTTSGTGSEVTQAAVIKDHEKHYKTAFMSPFLLPDA
                     AVLDPRMTLTLPPAVTAQTAMDALTHAMEAYTCLAKNPLSDAFARAAVALIGRHLLPV
                     MKNPADGTGRLALAIASNLAGVAFSNSMVGMVHCLGHATGSACGVPHGICMAILLPYG
                     LEYNLHKTGRWTAELLLPLAGRAVCDATPPEARAAKVIALVRQLNQDLHDASQGQHPR
                     TLKEVVDRGLNRMIPRERLPEIVAAARCDGAIFYNPEEMDADDFRMVLEAAWEGVALD
                     LSRIRKG"
     gene            4390..5886
                     /locus_tag="PLW16_05565"
     CDS             4390..5886
                     /locus_tag="PLW16_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70410.1"
                     /translation="MIEYDAVVIGAGNGGLAAAATLAKGGAKTLLLERHNIPGGCATS
                     FRRGRFEFEVALHQLSGLGTEAAPGPLRATLHGLDVLDRVEFVQMENMYRISLPGALD
                     LTLPTTRDGVVRALAERFPEEETAIRAFFDLVYDYFNQFIAIFFMRDPEVSREKYPLY
                     FRYALKPAQEVLDEHFRDPLLKLAASAYWCYIGVPPRQLPFGDLAGILYAYIEFKPWH
                     IKGGSQALSNALVDAFLRNGGEVRFNCPAARITVADGRVAGVVTADGEAIATGCIIAN
                     ASTLTTFTELIDRKDCPAAPLETMSGMTVGPSVVCLYIGLDCPPEALGIREETNFLCT
                     HADTDRAFAQYRSLDEPPGMVLLTCFNISNPEASPPGTSQIAIVDLKYADPWLAIPPE
                     RYAREKYRYAEGLLAIAEAQYPGFRSHIEEIEVSTPLTHMRYLNHPGGAIYGFDQYAK
                     DSRIFQPIKPALPGLFLAGAWAAEGGFQPTLMAGASAGRAALKTIRRK"
     gene            5896..7056
                     /locus_tag="PLW16_05570"
     CDS             5896..7056
                     /locus_tag="PLW16_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB70411.1"
                     /translation="MSAKNFLQEIDGYETLQQEIAVSRKYGVDYVARKGQVALAVNRL
                     HPARLTLRVNEILDETPSARTLRLVAAEGDLPPFQAGQYINVFVEAGGVRTSRPYSLS
                     SSPRQRAYYDITVRRVADGFVSEVLLDGTRAGDVLTSTSPAGTFHFNPLFHSPDMVCL
                     AGGSGITPFMSMIREIAEAGLDRHLTLLYGSRSEDDIIFADELRKLSSQHENIRYVPV
                     ISEPRAGCRERTGFLTADLIRDVLGGVDGRTFYLCGPAEMYAFCLPEIEKLAVPRRWV
                     RREMFGPPKRIADDPGWPAEVAPDRVFQAAVRGKRTIPARADEPLLVALERAGIVIPA
                     QCRSGECSLCRVKLLSGRVFQPAGVLLRQSDRRFGYIHSCMAYPLEDVEILI"
     assembly_gap    7444..7543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7544..8350
                     /locus_tag="PLW16_05575"
     CDS             <7544..8350
                     /locus_tag="PLW16_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70412.1"
                     /translation="YLVRGTAGAALIEAGVSATGDLVIGQLETLGVRPDVLVVTHPHG
                     DHLNGLPALREAFPTARVIAGPGAEAFCAHPKTGALLVNDDRYMSAFLAARGVAVRRP
                     PLAAPPSLAGSEVMRDGDVLDLGGVTLRFLEAKGHAPGNLAVHIPERTALIASDSLGY
                     RYLDRGFFPIFFTGFADYLATIDRMAALKPLILGLAHQGALTEAAADAAFGAAHAGAV
                     AVRDRLRRDERPDEAIVRDLFTEFYREELALYSEENILLCCALLLRRSRE"
     gene            8353..9531
                     /locus_tag="PLW16_05580"
     CDS             8353..9531
                     /locus_tag="PLW16_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70413.1"
                     /translation="MKIAILGAGPAGLYAALAAAERGFRTDLFEKRAIGEGIVCGECI
                     FDSLGILSRPGRGLLHPVEEVVLTARERHTLAIGRYRKLWMMDRRSWQEGLAEAAVAR
                     GVRLEAQTKIDPERLRRMQHEYDGIIDASGAPSVTSRAYGFSGRYLEDGLVAYQAVLR
                     GDFSTLWPRIAVGFLPDLPPERRPGYAWIFPKNARRANCGVIYTMRGSRRGGDPDLKA
                     LLADFLKSEGLAGAEILKTGGGIAAGRILPRLVYDRILLAGDAAGLTSPLHGGGMDLA
                     CLSGVLAVDALADGAHGAERYRPRLMGYVRHKLAVEALAIRKMRTLDFNAFDALLGGV
                     ASQSNLRRAATALRHPVLAFATLKWLFRRETAPAGLAERVWTPDAPEKKVRPASARDP
                     "
     gene            complement(9566..11356)
                     /locus_tag="PLW16_05585"
     CDS             complement(9566..11356)
                     /locus_tag="PLW16_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70414.1"
                     /translation="MRYKLDQPDNLIGFLEDSVARFPERPLFGTRLPDGEYEWITYRD
                     FGRRVDNLRGGLAAIGVGKGDMVGIIANNRVEWAAASFATHGLNARFVPMYEQELLHV
                     WEYIIRDSGIMVLFVSKPAILEQVREFPARIDTLKKIVLIEGEGPDTMAALEKTGEKH
                     PVPAVRPAADDIATLIYTSGTTGDPKGVLLSHANWTHNSHCRVKRYPGYDENDRVLSI
                     LPWAHTYGQGEMHTFLQIGASIALMGSTATLAGDMAKTRPTFIVAVPRVFNKIYEGLW
                     AKMDATGGLARKLFVMGVEAGKKRRSLAEKGRPDFLANLKFRIADRVVFAKIRERFGG
                     RLRAAMTASAMMNMEILLFFLDLGIPVYDCYGMTETSPAIAVNGPAAWRIGSVGKPIE
                     QVRVVIDSSVVEEGAQDGEIVCYGPNVMKGYHNKPEQTKEVMTEDGGIRTGDRGRLDK
                     DGFLFITGRIKEQFKLENGKFVFPAALEEDIMLLPSIDNVMVYGENRLYTIALVVPNF
                     FALERFAEAHGLPSDIKALIARDDVREMIAREIQDGLKGKFGAYEIPKKVILLTEGFS
                     LENGTLTQTMKLKRRVVLKKYEDLIAAAYK"
     gene            complement(11713..12531)
                     /locus_tag="PLW16_05590"
     CDS             complement(11713..12531)
                     /locus_tag="PLW16_05590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70415.1"
                     /translation="MDEVRDRCVVITGASSGIGKAISACFAREGAHLCLGAKPGETEV
                     LTAWARELERTHAVRTWTFPVDLALPDGPERFHAAVRGIGRPIDCFVNNAGVLWYGSF
                     AALTLEKQVEIVQVNVLAHMKLMRLFAEDMVKRRGGRIWNVSSVSAFQPCCHHAVYGA
                     TKAFVQSLSEAVHEELRGTGVTVCTLNPAYTDTRMIHGDGFPRRLWWFDVSGVRTPEE
                     TARIGYRAFKRGKALCIVGWQNRLIHTLLLRLTPRKLMAAMSYWVLQAKPSLHD"
     gene            12708..13397
                     /locus_tag="PLW16_05595"
     CDS             12708..13397
                     /locus_tag="PLW16_05595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPB70416.1"
                     /translation="MDARPLSDYTLVRSARRRKTLSLQVLPDGTVLVQAPRHTPKREI
                     EAFFRKKQPWIARKRREQAARRPHDRRIATGETFPYLGVSYPLEIVPPSDRKEPLVFT
                     GTKFVLHETAVRGGKARFIRWYRRQAMTYLAGRVAVFSVRLGVLPRGIRIGQARHRWG
                     SCSPENRLSFPWRLIMAPPDVVDYVVVHELAHIREKSHAPAFWALVDRAAPRHREHRR
                     WLKDHDHLLSL"
     gene            13471..14382
                     /locus_tag="PLW16_05600"
     CDS             13471..14382
                     /locus_tag="PLW16_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70417.1"
                     /translation="MTRKSKSDIRGAGPGGIDISFLRQLDQKGNGETGLFPLVFATNP
                     SMMAVTTADGQYREVNRAFCETTGYTRDELLGQNSMELGKWKGEQRQEILDHLSTDAR
                     IRNKEIYWRDKSGKLHCCLLSTEVIEFEGEKLVLSTLLDITERKQMEDELRKSKDSLA
                     LQAEKLEALNTALKVLLDQQEQNQRLTSARLKSNLQELITPYLEKLKDGQMAPMDRVS
                     LSVLESNLMDISSDFVYNLASCYPNLTPKEIQVANLVKEGKSSGEIAHVMGVSLGTVN
                     AHRNNLRRKMGLGKGGANLRSHLLAMK"
     gene            complement(14510..14585)
                     /locus_tag="PLW16_05605"
     tRNA            complement(14510..14585)
                     /locus_tag="PLW16_05605"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14550..14552),aa:Asn,seq:gtt)
     gene            complement(14656..15363)
                     /locus_tag="PLW16_05610"
     CDS             complement(14656..15363)
                     /locus_tag="PLW16_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPB70418.1"
                     /translation="MIEITLDGRKVKAQEGSTLLSHIRARNIDLPTLCQHDELSPFGA
                     CRLCTVEVKVNGKWELATACNTPVAQGMEVRTDSERAIAGRKLAASLLYYKYPKTRVV
                     RDLAEKLGIAVAAEAADAKECVLCGLCARTCREIVGVAALSFEDRGLGRDVDEPRIAF
                     DANACIGCGSCAYVCPTGHVKMETTGDKRIIWDKAFKMATCQVCGRPFAPEDQLKYIS
                     VRTGVPMSRLKTCMSCR"
     gene            complement(15483..>16469)
                     /locus_tag="PLW16_05615"
     CDS             complement(15483..>16469)
                     /locus_tag="PLW16_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70419.1"
                     /translation="DLVRRLGVEIRLNEPVGRIDPGKLKKAGYAAVFIAVGAHAGNAI
                     GAEGEDAGYDGFMEGIEFLRRISLGQKIASRRKVVIIGGGNVAIDCARTCLRLGFGEV
                     EILYRRSLAEMPARAEEIAEAQEEGVKIRFLAAPLKVVAKNGKVTAIECIKMKLGEPD
                     ASGRRRPVPVKGSEFSVKTEMIIPAIGQNPALPAVAGTEALAVAKGNTVAVDAVTLGT
                     NLDGVFAGGDCVTGPATLIEALDMGNRAARSIDLYLRGETPAAERPFAGIDTRAQRTK
                     GFVSPAASGEAALLQAKTRIAGFDEVEGGFDAAAAIREANRCLRCYRLMVWA"
     assembly_gap    16470..16569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16570..17150)
                     /locus_tag="PLW16_05620"
     CDS             complement(<16570..17150)
                     /locus_tag="PLW16_05620"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB70420.1"
                     /translation="MKTVSFSSWGGKVIDNRKGRPAKAAKVEAPLTIDGGAVTALMGW
                     NGIVVADPSVDILSLTLAYLEEARKLSCGECSVCLIGIDRMLDLLRARAAGKGSKADL
                     DEMDEIVRGVAANAKCNFGRAAALNPVGDALKYFRADFANPAASAGKTTAKTYRVAVT
                     APCMEACPATLDIPGYIELIRNGRFGESLNLIR"
     gene            complement(17189..18163)
                     /gene="porB"
                     /locus_tag="PLW16_05625"
     CDS             complement(17189..18163)
                     /gene="porB"
                     /locus_tag="PLW16_05625"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HPB70421.1"
                     /translation="MMSTAKPISIVENFEIYAPKLVDKEEFFCSGHRACQGCGEALAI
                     RLMCKALGKDTVIANATGCMEVIATPYPATAWTLPWIHVAFPCAAAVGAGVEAGLKVL
                     RRKGVNPDRYIKTVTIGGDGGTVDIGLQALSGAMERGHDMLYVCFDNEAYMNTGIQRS
                     SATPFGASTTTAPAGKASIGNKTWKKNVPEIMVAHNIPYVATASPSYPLDFMNKVKKA
                     RSIQGPTYIHCLSVCPTGWRAKSEECIKLGRLAVETGVFPLYEVENGVYRLTVEMPDP
                     LRPVTDYIKAQGRFRHLTPDEIRDIQRRVNLEYRKLMNKVEHSLSWTE"
     gene            complement(18160..19320)
                     /locus_tag="PLW16_05630"
     CDS             complement(18160..19320)
                     /locus_tag="PLW16_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPB70422.1"
                     /translation="MSKRIGMEIALAAAEAVALCRPDVIAAYPITPNTHIPEHLSDIV
                     AEGRLDAEFICVESEHSAMSACAGASGTGARAFTATSSQGLLYMYEVLGIVSSMRLPV
                     VMAIGNRAISGPINIWNDHSDIMTMRDLGWLSLFAANGQEAVDMMIQSFKIAEDAAVQ
                     LPINVNLDGFQLTHMVEALFLPDQKEVDRFLPRRKPYAALHPDHPVTMGALGMPDIFT
                     EATKARDQALVDSRKVILKVWKEWEKQFGRRYEPIEAYKTKGADVVMMTMGSMGETAE
                     IAVDELRKDGVPVGLLKLKLWRPFPFEEMKKAVRGVKTLIVTDRCCSLGGPGGPLAAE
                     VRSALYAEAARPVIENALIGLGGRDVTVDDFKQMFRQAGKSKGIYEFYGVRG"
     gene            complement(19324..19614)
                     /locus_tag="PLW16_05635"
     CDS             complement(19324..19614)
                     /locus_tag="PLW16_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB70423.1"
                     /translation="MAQKKWPETWQEVTPGCMIFEGGNSREYHTGSWRAQRPVWDNAK
                     CIKCGICYIFCPEACVKEDARGYFAADLDYCKGCGICAHECWPGAITMIEEG"
     gene            complement(19639..20184)
                     /locus_tag="PLW16_05640"
     CDS             complement(19639..20184)
                     /locus_tag="PLW16_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB70424.1"
                     /translation="MLEVRWHGRGGQGAVTSVELLALAAIGEGRFAQGFPSFGPERRG
                     APVAAFNRVDEKRIRRRCVIASPDVVVVLDESLIGLVNVTDGLKPDGVLVVNTSRTAA
                     EIRKALNYKGRLAMVDASAIAVQELGTPITNTTMLGALIRATKVVKLASVAAPLEHRF
                     GRIAPRNQKALQRAYEEVKIH"
     gene            complement(20420..20986)
                     /locus_tag="PLW16_05645"
     CDS             complement(20420..20986)
                     /locus_tag="PLW16_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPB70425.1"
                     /translation="MNFNALMKNLPRRIAAPLAPEICEDLKVGDRLLIGGAVYTARDA
                     AHQRIAAALARGDDPPFDLDGAVIFYAGPSPAPPGRPIGAIGPTTSYRMDPFTPLLLE
                     RGLKGMIGKGRRSEDVVRAIARHGAVYFGAIGGIAALMAKCVQRAEIVAYEDLGPEAV
                     RRLEIVDLPVIVVNDARGGDLFARTAGG"
     gene            complement(21001..21852)
                     /locus_tag="PLW16_05650"
     CDS             complement(21001..21852)
                     /locus_tag="PLW16_05650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPB70426.1"
                     /translation="MTTARDLSARCVTETVRELFLTANTVLPADVLAALEGAGERETN
                     GLGRYVLERILENARVAHEEGMPLCQDTGLAVVFLEIGQDLHIVGGDLETAVQKGVRR
                     AYRDGFLRASVCDPLTRHNTGDNTPAVLHTAIVPGDRLRITALPKGGGSENMSSAAML
                     VPAAGEAGIRAHVLDRVRQAGPNPCPPVIVGVGIGGSLDQAPVLAKQALLRPVGRPHP
                     TDARLAAMEAALLEEINRLDIGPQGYGGEVTALAVHVEMRPCHIACLPVAVNLQCHAA
                     RHRTAIL"
     gene            complement(21876..22475)
                     /gene="recR"
                     /locus_tag="PLW16_05655"
     CDS             complement(21876..22475)
                     /gene="recR"
                     /locus_tag="PLW16_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPB70427.1"
                     /translation="MTGYALPIQKLIRELSRLPGIGERTATRLATHILRAPAEDARRL
                     AESIVEVKEKIHLCPVCFNLAEGEQCDICRDGSRDRGQICVVEEPDSLMAVEQSGSFR
                     GTYHVLHGVLAPLDGVGPEQLRLQELLKRVQGNGVRELIIATNPSVQGEATALLITRL
                     VKDQGVSVTRIAQGVPVGGDLKYTDRMTLARSIEFRRGM"
     gene            complement(22486..22800)
                     /locus_tag="PLW16_05660"
     CDS             complement(22486..22800)
                     /locus_tag="PLW16_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPB70428.1"
                     /translation="MSHNIGNIMKQAKKLQERMGQLQAEMEQKTVTATSGGGMVTVTV
                     SGKGELVSLKIERDVVNAEDVEMLQDLILAAVNEGVRKAQEMAQSEMMKLTGGLNIPG
                     LT"
     gene            complement(22844..24535)
                     /gene="dnaX"
                     /locus_tag="PLW16_05665"
     CDS             complement(22844..24535)
                     /gene="dnaX"
                     /locus_tag="PLW16_05665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPB70429.1"
                     /translation="MSAEYRVLARKWRPQIFEDVLGQEHVVQTLKNAILHRRIAHAFI
                     FSGPRGVGKTSVARIMAKALNCETGPTDTPCNACVNCKEITDGIALDVREIDGASNRG
                     IDEIRELRENVRFSPASCRYKIYIIDEVHMLTREAFNALLKTLEEPPPHVIFMFATTE
                     THKVPATILSRCQCFDFRRISLKAIASSLKAIAAAEGIAISDTGLAWIAEAGDGSLRD
                     AQSVFDQVISYGGATIADGDVETLLGLTDRRFVLDLSRAIVARDAARCLKIVEEGYFA
                     GLDMKVFYQMLLVHFRNLLMTKIAGDAPGLIEVSDADLAHLKALAGEVSRETLQRLLD
                     ILLAEEEAVRRSRDPRINLEAALVRMAYLEPLLPIDVILDRMEGIERRLTGAPPASRE
                     GRPAASPAYDGPEPPAARAVREPVDPAAGPERREDPSAPDPAGDDWEGFKAFVRQTSF
                     PLGSKIEKGRILDRGGNELKIAMPKEYAFLLGPGEKKRLEEIARTFFRQDSLRVRIEV
                     VANGPAGAANGNGAARNGKNGDLKRQALDHPLVKKALDVFPGAMIQEVIIRNPEG"
     gene            complement(24562..24659)
                     /gene="ffs"
                     /locus_tag="PLW16_05670"
     ncRNA           complement(24562..24659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLW16_05670"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         3952 a         8448 c         7985 g         4184 t
ORIGIN      
        1 ccctcggaag cgacggaacc tgcaccttca cggagccgtg atcgccgccg gcacgggcgg
       61 aggcatttgc cgcctcgtgg gcttcgcctg agccgcgtga tcgccagcgg cgcgggggtg
      121 ggatgcccct agctgtcggg aatgccgtag agctccttga ggacgccgtc gaagtaatcg
      181 ggaagatagc ggtcccacag cttcaccggc agcgtctcca gttcccgccg gggagaggtg
      241 gcaaaggcga agaggtattc gagcagatac tccatgttct ttaccatcga cccccggtac
      301 tcgcggcgca ggcgctgctc gatgatccgg tcctcgtgca tgcagcgccc gacctcgctc
      361 tccagggtct cctggtagtc ggaaacgatg atctccagat ggacgtccgg ctcgccggcg
      421 cggacgaagg ccgcgaattc ccggacggtc gcctccggat cgtccccttt ccgcttcagg
      481 atctcgacga cgccggcgct caggggctcc gccagcagag cctcccgcgg cggctcgagc
      541 cggacgatgt agttgaccgc attgtaatgc ccggtgtgct gctccttttc caggaccgaa
      601 cacccgtcca gggacgcgag cacggactcc agttcgccca ggcgctcgcc cggcaggatg
      661 agcttgagcc ccgccacgtc gttgatggcc agcggctcgc cgtcctcgat cgcctgcctg
      721 tggcggaggt tcgaaaggag gcgcgcctcc gcgcgcagga attcgtcgcg catgtcctcc
      781 ggcgtcgtca cgagctggcc cggttgaatg cccagcctcc gggcgcgggc ctcggccagt
      841 tgatccgcgt gggcccggat gcggtcgaag atccggtccg tgatccgccg ggccgccttt
      901 tcggccaggc ggcgatcgcg cttgatccgc atcgaacctg caagggccgc cgggtcctcc
      961 cggccgacaa agaccagcca gccgtcaaac ggggtctcgc gaaaataatg tccgggaaag
     1021 gcggcatagt cacggatcag ccgatcgatt cgttcattac ggtaaggcgg acgggcgacg
     1081 aggcaatcct tgagctcgcc cttcaccgtc accggctgaa agaccggatc cattccgaaa
     1141 agcccgcgga aaagacgcac ggcgaactcc cggaggtagc gggcgatata ctcctggttg
     1201 aaatggacga gccgggtgac ccgcccggca tcggcggggt gaacgtcact gtacatccat
     1261 cgccggatga tctccagcaa ctcctcccgg tgcagatagc tgttgatccg gatcatggct
     1321 tcccccttcg agccagggaa cactctacac agatcgggtc cgttgcgcaa cccggaaatc
     1381 ggaccgacac ccgcgggcgc acgggatctt tcgggatgga cttgcccccg aaaagttggt
     1441 atgcagggat ccatcgtggc atgggttctt tcagggcaac acccatcttt catgacctgc
     1501 cttaaggagg gaaagggatg aacgattacc gggaggcgcc ggcgggaaga aaatccctga
     1561 aacgggcgta cgtggccgtc atgctgtggt tcgtcggacg ggcgcttcag gtggccgccc
     1621 gggcggatgc cgaggtccgt cgggagttcg aagccctgcc ggaggggttc accttcgcca
     1681 tgggggtcct gcccgcgggc ccctggatga tcgtgggcaa acgggcgggc cggatccgct
     1741 acctgggctg gcgtcccgag gggatcgccc tcgacctcac cctgaagatc aagcacctgg
     1801 aggcggcgat cctgatgttc accttccggg agagcaccgc cgtcgcgacc gcccgcaacc
     1861 gcatgatcgt ggacggggaa gtccccccgg cctgcgcgat cgtccggatc ctcgacatcg
     1921 tcgaggtgta cctcctgccg aagttcatcg cccgcctcgc cgtcaagcgc tatccccgct
     1981 ggtcctggac ccgcaaactc gtcggccgga cccggatcta tctcggggcc gtcctcggcc
     2041 gctgaaagga gggtgacatg gagatcccgc gtcatttcga attcttctgc ccgatccgga
     2101 tcttttccgg aagccgggcc ctggaacatc ttcccggcga actgcgggcg ctgaacgcct
     2161 cccgcccgct cgtcctcgcc acgcagggga ccgccgaccg ggggctcacg cggattctgg
     2221 tcaaggcctt cgcggattcc ggcatgcccc tcgggctcca cgaagccgca ccggagtccc
     2281 cggacgcggc ctggaccgcc cgggtgaagc atctgttcgt ggagcaccgc catgacgccc
     2341 tcctcgtgat gggcgacggg cccctcgtca cggccgccaa gatcctcaac atcgccgtct
     2401 cgatcggccc ggaggacgat ctggccgccg cgtccgggga caaccgactg acccggccac
     2461 tcctgccctt cgtcgtgatc cccaccggcg tcgactgcga ggcggtggcc tccccgacgg
     2521 ccgcgctggg gccctggacc ttcaggtctc cggcgctgat ggcggccctg gtcgccctcg
     2581 atccccgcac gatccgcccc ggcgacgccg gacgcgatgc ggcggtcctg tcggtcgccc
     2641 gcgcgcacgc cgcccgggta atcgccgcgg cgccccggaa ccccgtgggc gaggcctatg
     2701 cccgggccgg gctcgcactt ctggaccgaa acggatcgaa ggccggcaag ggggaacggt
     2761 gggcgctcgc caacggcgtc ctcctggcgg ggtgcgccca cgcccaggcg gagccggcgg
     2821 gaccggacgc ggcggaggcc gccttcacgg gagaacccgc cacagcggag gccgcctccg
     2881 cctacctgcc cgactattac gaattcctga acggcggacg gatcgtcgcg ggtcacgacg
     2941 ccctgaagcg gattcccacg attctcgctg atctgggcgc aaaaaacccc ctcatcgtca
     3001 ccgaccgcgg cgtcgccggc gccggcctgg tggaggtcgc ccggaacgcc ctccgggacg
     3061 ccctggcgct ggagacgatc gccgacgacg tccccgtgga ttcggacatc aaggcggtcc
     3121 gccggatcgc cgaactctac cggcagcgcc actgcgacag cttcatcgcc gtcggcggcg
     3181 gctccgtcat ggatacggcc aagggcgtca acatcgtcgt ctccgagaac gcccgcgacc
     3241 taatggccta cagcggcgtc ggcgcgatcc gcagccgcct gcggcccctg atcgccgtgc
     3301 cgaccacctc cggcacgggc tccgaggtca cgcaggccgc cgtgatcaag gaccatgaaa
     3361 agcattacaa gacggccttc atgagcccct tcctgctgcc cgacgccgcc gtcctcgacc
     3421 cccggatgac cctcaccctg ccgcccgccg tcaccgcgca gacggcgatg gacgccctga
     3481 cccacgccat ggaggcctac acgtgcctcg caaagaaccc gctgagcgac gccttcgcgc
     3541 gcgcggcggt ggcgctgatc ggccggcacc tgctgcccgt catgaagaat ccggcggacg
     3601 ggacgggacg cctggcgctc gcgatcgcct ccaatctggc cggggtggcc ttttccaatt
     3661 cgatggtggg gatggtccac tgcctgggac acgcgaccgg ctcggcctgc ggcgtcccgc
     3721 acgggatctg catggcgatc ctcctgccct acggcctgga gtacaacctc cacaagaccg
     3781 gccggtggac cgccgaactc ctcctgcccc tggctggccg cgcggtctgc gacgccacgc
     3841 cgccggaggc gcgggccgcg aaggtcatcg ccctcgtgag gcagctgaac caggacctgc
     3901 acgacgcctc ccagggccag catccccgca ccctgaagga ggtcgtggac cgcggtctga
     3961 accgaatgat cccccgggag cggctccccg agatcgtcgc ggcggcccgg tgcgacggcg
     4021 cgatcttcta caacccggag gagatggacg ccgacgattt ccggatggtc ctggaggccg
     4081 cctgggaggg cgttgccctc gacctctccc ggatccggaa gggctgacgg cgcgccacgg
     4141 ggatccggtt ggccgcgttg accgtcggcg ttgcggccaa gaccgccccg aaacggatgc
     4201 ggcatcgaag cgccgtcccg ggttttgcac gcatcgagaa tccggatccc gcatgcgcac
     4261 aggggagacg ccgtcacggt gcccctgaaa gggcgggacg ggcctgatcc cgcgtgcgcg
     4321 cgggagcaac gaagcataag aaaaatgggg cgggctggga cgctatttat taagaaaggg
     4381 aggactggta tgatcgaata tgatgcggtt gtcatcggag cggggaacgg gggcctggcc
     4441 gccgccgcaa ccctggcgaa gggcggggcg aagaccctgc ttctggagcg gcacaacatc
     4501 ccgggcggct gcgccacgag cttccgccgc gggcgcttcg agttcgaggt cgccctccac
     4561 cagttgagcg ggctcggcac ggaggccgcg ccggggccat tgcgcgcgac cctgcacggg
     4621 ctcgacgtcc tcgaccgggt cgagttcgtt cagatggaga acatgtaccg gatctccctc
     4681 cccggggcgc tcgacctcac cctgccgacc acgcgcgacg gcgtcgtccg ggcgttggcg
     4741 gagcgcttcc ccgaggagga gaccgcgatc cgggccttct tcgacctcgt ttacgactac
     4801 ttcaaccagt tcatcgccat cttcttcatg agagacccgg aggtctcccg ggaaaaatac
     4861 ccgctttatt tccggtacgc cctcaagccc gcgcaggagg tcctcgacga gcatttccgg
     4921 gacccgctcc tgaagctggc cgcgagcgcc tactggtgct acatcggggt gccgccccgg
     4981 cagctcccct tcggcgacct ggccggcatc ctctacgcct atatcgaatt caagccctgg
     5041 cacatcaagg gcggctcgca ggccctgtcc aacgccctcg tcgacgcctt cctccggaac
     5101 gggggcgagg tccgcttcaa ctgcccggcc gcgcggatca ccgtggcgga cggccgggtc
     5161 gccggcgtcg tcaccgcgga cggcgaggcg atcgcgaccg ggtgcatcat tgccaacgcc
     5221 tcgacgctca ccaccttcac ggagctgatc gaccggaagg actgcccggc ggcgccgctc
     5281 gaaaccatga gcggcatgac cgtggggccg tcggtcgtct gcctctacat cgggctcgac
     5341 tgcccgcccg aggccctggg catccgggag gagacgaact tcctgtgcac ccacgccgac
     5401 acggatcgcg ccttcgccca gtaccggagc ctcgacgaac ccccggggat ggtattgcta
     5461 acctgcttca acatctccaa ccccgaggcc tcccccccgg ggacctcgca gatcgcgatc
     5521 gtggacctga agtacgccga cccgtggctc gccattccgc cggaacgcta cgcgcgggag
     5581 aagtaccgct acgcagaggg cctcctcgcc atcgccgagg cccagtatcc cgggttccgg
     5641 tcgcacatcg aggagatcga ggtctccacg ccgctcaccc acatgcgcta cctgaaccat
     5701 cccggcgggg cgatctacgg attcgaccag tacgccaagg acagccggat cttccagccg
     5761 atcaaaccgg cgctccccgg cctctttctc gccggggcat gggcggcgga gggcggcttc
     5821 cagcccaccc tgatggccgg ggcctcagcc ggccgcgcgg ccctcaaaac cattcgcaga
     5881 aagtgaggtg acgccatgag tgcaaaaaac ttccttcaag agatcgacgg ctacgagacg
     5941 cttcagcagg agatcgccgt cagccgcaag tacggcgtcg actacgtcgc ccgcaaggga
     6001 caggtggcgc tcgccgtcaa tcggctgcac cccgcccggc tcaccctccg ggtgaacgag
     6061 atcctcgacg aaacgccgtc ggcccggacg ctccggctcg tggccgccga gggggacctg
     6121 ccccccttcc aggccgggca atacatcaac gtcttcgtgg aggcgggcgg cgtccggacg
     6181 agccggccct acagcctctc ctcgtccccg cgccagcggg cctattacga catcaccgtc
     6241 cggcgggtcg cggacggctt cgtctcggag gtcctgctcg acgggacgcg cgcgggggat
     6301 gtcctgacga gcacctctcc ggcggggacc ttccatttca acccgctgtt ccacagcccg
     6361 gacatggtct gtctcgcggg ggggagcggc atcaccccct tcatgagcat gatccgcgag
     6421 atcgccgaag cgggcctcga ccggcacctg acgctcctgt acgggagccg gagcgaggac
     6481 gacatcatct tcgccgacga acttcgaaag ctctcatcac agcacgaaaa catccgctac
     6541 gtcccggtca tctccgaacc gcgggcggga tgccgggaac ggaccggctt tctcaccgcc
     6601 gacctgatcc gggacgtcct gggcggcgtg gacggccgga ccttctacct gtgcggcccc
     6661 gccgagatgt acgccttctg cctgccggag atcgaaaagc tggccgtccc gcggcgctgg
     6721 gtgcgtcggg agatgttcgg tccgccgaag cgcatcgccg acgacccggg ctggcccgcg
     6781 gaggtggccc ccgaccgcgt gttccaggcg gccgtccgcg ggaagcggac gatccccgcc
     6841 cgggcggacg agccgctcct cgtcgccctg gagcgcgccg gcatcgttat cccggcccaa
     6901 tgccggtccg gtgagtgcag cctctgccgg gtaaagctcc tgagcggccg cgtcttccag
     6961 ccggcggggg tcctgctgcg ccaatcggac cgccggttcg gctacatcca ctcctgcatg
     7021 gcctaccccc tggaggacgt ggagatcctg atctgaagac gggcgtcggt agcgacgaaa
     7081 ggtgcaagcc ccggaccgca ggccgggggc gatccggacc ttctcggaca gggaggcgtc
     7141 tcacggcaac gcgcatggga ttttttcggg gcccggcccc cgcggtggcg cgacggaggg
     7201 gaaacggccc cggcgcctgg ccgcagcagg tccggcgctc cgggggatca cgatgccaag
     7261 cttaaacgga tatctcaaca ggaccggcac ggggtgaaaa cgacatgcgg aagtcaacgg
     7321 cggaggcgga aatcccggct cgtctggccg acggcctgtg gatcctgggg caccccttct
     7381 tcaatctgta tctcgtccgg ggaacggccg gcgcggccct gatcgaggcg ggggtatcgg
     7441 ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatctcg tccggggaac
     7561 ggccggcgcg gccctgatcg aggcgggggt atcggccacg ggggacctcg tgatcggtca
     7621 gctcgaaacc ctcggcgtcc ggccggatgt cctggtcgtc acgcatcccc acggcgacca
     7681 cctcaacggc ctgccggccc tgcgggaggc gttcccgacc gcgcgggtga tcgccgggcc
     7741 gggcgcggag gctttctgcg cccatcccaa gaccggggcg cttctggtca acgacgaccg
     7801 ctacatgtcg gcgtttctcg ccgcccgggg cgtcgcggtg cgccggccgc cgctggcggc
     7861 gccgccgtcg ctcgcggggt ccgaggtcat gcgcgacggg gatgtcctgg atctgggcgg
     7921 ggtgacgctc cgcttcctcg aggcgaaagg ccacgccccg gggaacctcg ccgtccacat
     7981 ccccgaacgc acggccctga tcgcctccga cagcctgggt taccgctacc tcgaccgcgg
     8041 cttcttcccc atcttcttca cgggattcgc cgactatctc gcgacgatcg atcggatggc
     8101 ggccctcaag ccgcttatcc tggggcttgc acaccagggg gcgctgaccg aggcggcggc
     8161 ggacgcggcc tttggcgccg cccacgcggg cgcggtcgcc gtgcgggacc gtctccgccg
     8221 ggacgaacga cccgacgagg ccatcgtccg ggaccttttc accgaattct accgcgagga
     8281 gctcgccctc tactccgagg agaacatcct gttgtgctgc gccctcctgc tgcgccgcag
     8341 ccgggaatga ccatgaagat cgccatcctc ggcgccggtc ccgccggcct ctacgcggcc
     8401 ctggccgcgg cggaaagggg tttccggacg gatctgttcg aaaaacgcgc catcggggag
     8461 ggcatcgtct gcggggagtg catcttcgat tccctgggga tcctgagccg gcccgggcgg
     8521 ggcctgctcc atcccgtgga ggaggtcgtc ctgacggccc gagagcgcca cacgctggcg
     8581 atcggccgct accggaagct ctggatgatg gaccgcagga gctggcagga gggactcgcg
     8641 gaggccgccg tcgcccgcgg cgtgcgcctg gaggcacaga cgaaaatcga cccggagcgc
     8701 ctccggcgga tgcaacacga gtatgacggg atcatcgacg cgagcggcgc gccgtcggtc
     8761 acctcccggg cgtacgggtt ctccggccgc tatctcgagg acggcctcgt ggcctatcag
     8821 gcggtgctgc ggggcgactt ttccaccctc tggccccgga tcgcggtcgg cttcctcccc
     8881 gacctccccc cggaacgccg gccgggttac gcctggatct tcccaaagaa cgcccgacgg
     8941 gccaactgcg gggtcatcta cacgatgcgg ggttcccgcc gcggcggcga tccggacttg
     9001 aaggcgcttc tggcggactt cctgaagtcg gaaggcctgg cgggggcgga gattctgaag
     9061 accggcggcg gcatcgcggc gggacggatc ttgccgcgcc tggtctacga ccggatcctc
     9121 ctggcggggg atgcggcggg gctgacctcg cccctgcacg gcggcggcat ggacctggcc
     9181 tgcctgagcg gcgtgctggc cgtcgacgcc ctcgcggacg gcgcgcatgg agccgaacgc
     9241 taccggccgc ggctgatggg gtacgtccgt cacaagctgg ccgtcgaggc cctggccatc
     9301 cggaagatgc ggacactcga cttcaacgcc ttcgacgccc tgctcggcgg cgtcgcctcc
     9361 caaagcaacc tccgacgcgc cgcgacggcc ctgcgccacc ccgtgctcgc cttcgccacg
     9421 ctgaagtggc tgttccgccg ggagacggcg cccgccggcc tcgcggaaag ggtttggacg
     9481 ccggacgcgc ctgagaaaaa ggtgcgaccg gcatccgccc gagacccgta accggcgtcc
     9541 gggacgatga ttgggcccgc cgccgctatt tgtacgccgc tgcgatcagg tcttcatact
     9601 tcttgaggac gacgcggcgc ttgagcttca tcgtctgggt cagcgtcccg ttctccagtg
     9661 agaagccctc ggtcaggagg atgaccttct tcggaatctc ataggcgccg aattttccct
     9721 tcaggccgtc ctggatctcc cgggcgatca tctcccggac atcgtcccgc gcgatcaggg
     9781 ccttgatgtc cgagggcagg ccgtgcgcct ccgcgaaacg ctccagggcg aagaagttcg
     9841 ggacgacgag cgcaatcgtg tacagacggt tctcgccgta aaccatgacg ttgtcgatcg
     9901 agggcaggag catgatgtcc tcctcgaggg cggcggggaa gacgaacttc ccgttctcca
     9961 gcttgaactg ctccttgatc cggccggtga tgaagaggaa gccgtccttg tcgaggcggc
    10021 cgcggtcgcc ggtgcggatg ccgccgtcct ccgtcatgac ctccttcgtc tgctcgggct
    10081 tgttgtggta gcccttcatt acgttcggtc cgtagcagac gatttcaccg tcctgggccc
    10141 cctcttccac aaccgacgaa tcgatgacga cccggacctg ctcgatgggc ttgccgacac
    10201 tgccgatccg ccaggcggcc gggccgttga cggcgatggc cggcgaagtc tccgtcatgc
    10261 cgtaacagtc gtagaccgga atcccgaggt cgaggaagaa caggaggatc tccatgttca
    10321 tcatggcgct tgccgtcatc gcggcgcgca gccgaccgcc gaagcgttcg cggatcttcg
    10381 cgaagacgac ccggtcggcg atccggaatt tgaggttggc gagaaaatcc ggccgcccct
    10441 tttcggccag cgaccgccgt ttcttgccgg cctcgacccc catgacgaag agcttccggg
    10501 ccagaccgcc ggtggcgtcc atcttcgccc acagcccctc gtagatcttg ttgaagaccc
    10561 tcggcacggc gacgatgaag gtcgggcggg ttttggccat atcaccggcc agggtcgcgg
    10621 tgctccccat cagggcgatc gaggccccga tctgcaggaa ggtgtgcatc tccccctggc
    10681 cgtaggtgtg ggcccagggc aggatggaca gcacgcggtc gttctcgtca tagcccggat
    10741 agcgtttgac gcggcaatgg gaattgtggg tccagttggc gtgggacagg agcaccccct
    10801 tggggtcgcc cgtcgtgccg gaggtgtaga tcagcgtggc gatgtcgtcg gccgcgggcc
    10861 ggaccgcggg caccgggtgc ttctcgcccg tcttttccag cgccgccatc gtatccggcc
    10921 cctcgccctc gatcaggaca atcttcttca acgtgtcgat ccgggcggga aattcccgga
    10981 cctgctccag gatggcgggt ttcgagacga acaggaccat gatcccactg tcccggatga
    11041 tgtactccca gacgtggagg agctcctgct cgtacatggg cacgaagcgg gcgttcaggc
    11101 cgtgggtggc gaaggaggcc gcggcccact ccacccggtt gttcgcgatg atgccgacca
    11161 tatcgccctt tccgacgccg atcgcggcga gccccccgcg caggttgtcc acgcggcgtc
    11221 cgaaatcacg gtaggtgatc cattcgtact ccccatcggg cagccgtgta ccgaataggg
    11281 gccgttcggg aaagcgcgcc acgctgtcct ccagaaatcc gatcaaattg tccggttggt
    11341 cgagcttgta tcgcatgcat cctcctccca catgaaatcg atgcgaccgg aacggggtta
    11401 caccctgcgg atgggtccgt ccggtccgcc gtcgagacgc cggcaggatc cgggaaacgc
    11461 acgcccccgc tctgaccgat ggtcatcctc ttaccgctgg ctccatacga caatccggta
    11521 taaatgtcaa gaaatattga ttatgttctg gatttgctga cctgagctga gcgaaattca
    11581 atgcaggtaa ggggcgacag gcaccgtgcc caaggccgcc tcccgggcag ccccccggcg
    11641 cgaatccgga ttccccgcgg gaggccccgg aggcgcccgg ccagcaccgg tgtggtaccg
    11701 ggacgcggat cgtcagtcgt gcagcgaggg cttcgcctgc agaacccagt aggacatcgc
    11761 ggccatgagt ttgcggggcg tcagcctcag gagcagggtg tggatcagcc ggttctgcca
    11821 gccgacgatg cacagggcct tgccccgctt gaaggcgcgg tagccgatcc gggcggtctc
    11881 ctcgggcgtg cggacgccgg agacgtcgaa ccaccagagg cgcctgggaa acccgtctcc
    11941 gtgaatcatc cgggtatccg tgtaggcggg gttcagcgtg cagacggtca cccccgttcc
    12001 gcgcaactcc tcgtggaccg cctcgctcag gctctggaca aaggccttcg tcgccccgta
    12061 gacggcgtgg tggcagcagg gctgaaacgc ggagacggac gagacgttcc agatgcggcc
    12121 cccccggcgc ttcaccatgt cctccgcgaa caggcgcatg agcttcatgt gcgcgagcac
    12181 gttgacctgg acgatctcga cctgtttttc gagcgtcagg gcggcgaacg acccgtacca
    12241 gagaacgccg gcgttgttga cgaagcagtc gatgggccgc ccgatcccgc ggacggcggc
    12301 atggaagcgt tccggcccgt ccggaagcgc gagatcaacg gggaaggtcc atgtccggac
    12361 cgcatgggtc cgttcgagtt cccgggccca cgccgtcaaa acctcggttt cccccggttt
    12421 cgcccccagg cacaggtgcg ccccctcccg cgcgaagcag gccgaaatcg ccttcccgat
    12481 ccccgacgac gccccggtga tcacgacaca ccggtccctc acctcatcca tccccatgtc
    12541 cccctcacgt caccttttcg agaatatgaa tgcacatggc gacctccccg gcgccgacgt
    12601 tgccgctatt tttagcaggt accacacctg gagaccttcg cgcaacgtcc aaatcgggac
    12661 cgaaggcgcg gcttgaaaaa agggatactc tttgctaaac atggggcatg gatgcccgtc
    12721 cgctttccga ttataccctg gtgcgcagcg cgcgacggcg gaaaaccctc tccctgcaag
    12781 tcctccccga cggcacggtc ctcgtgcagg ccccccgcca caccccgaag cgcgagatcg
    12841 aggcgttctt ccggaagaag cagccctgga tcgcccggaa acgccgggaa caggcggcgc
    12901 gacggccgca cgacaggcgc atcgccacgg gagaaacctt cccctatctg ggcgtctcgt
    12961 acccgctgga gatcgtcccc ccgtcggacc ggaaggaacc gctggttttc acgggcacga
    13021 aattcgtgtt gcacgaaacg gccgtccgcg gcggcaaggc acggttcatc cgctggtacc
    13081 ggcggcaggc catgacgtac ctggcgggcc gggtggcggt tttctccgtg cgcctgggcg
    13141 tcctgccccg cggcatccgg atcggccagg cacgacaccg ctggggctcc tgttcacccg
    13201 agaaccgcct gtccttcccc tggcgattga tcatggcccc cccggacgtc gtcgattacg
    13261 tcgtggttca cgaactggcg cacatccgcg agaagagcca cgcccccgcc ttctgggcgc
    13321 tcgtcgaccg cgccgccccc cggcatcggg agcaccggcg ctggctcaag gaccacgacc
    13381 acctgctgtc cctgtgattt gaatgaattc ttgacacaaa ggtaacgtcc cgctatagtt
    13441 cgccaaattt tgttcaggaa aggcggaatc gtgaccagaa aaagcaagtc cgacattcgg
    13501 ggtgccggac cgggtggcat cgatatctct tttctcaggc aactggacca aaagggcaac
    13561 ggcgaaacag ggctcttccc cctggtcttc gccacgaatc cctccatgat ggccgtgacc
    13621 acggccgacg gtcaatatcg cgaggtcaac cgggcctttt gcgagacgac gggctatacg
    13681 cgcgacgagc tactcggtca aaactccatg gaactcggga aatggaaggg ggagcaacgc
    13741 caggaaatcc tcgatcacct gtcgacagac gccaggatcc gcaacaagga gatctattgg
    13801 cgggacaagt ccggaaagct ccattgttgc ctcctgtcga ctgaagtcat cgaatttgaa
    13861 ggagagaagc ttgtcttaag cacactcctg gacatcacgg aacgcaaaca gatggaggac
    13921 gagcttcgga aaagcaagga ttccctagcc ctgcaggccg aaaaactcga ggccctcaat
    13981 acggccctca aggtccttct ggaccagcag gaacagaatc agcgcctgac atccgcgcgg
    14041 cttaaatcca acctccagga gctcatcacc ccctacctgg agaaactgaa ggacggccag
    14101 atggcgccga tggatcgggt ttccctgagc gtcctggaat ccaacctgat ggacatctcc
    14161 tcggacttcg tgtacaacct cgcctcctgc tatcccaacc tcacgcccaa ggagatccag
    14221 gtcgcgaacc tcgtcaagga ggggaagtcc tcgggcgaga tcgcgcacgt gatgggcgtc
    14281 tctctgggca cggtgaacgc ccaccgcaac aacctccgca ggaagatggg actgggcaag
    14341 ggcggcgcga acctccgctc gcacctgctg gcgatgaagt agcgccccca ggcgggccgg
    14401 cggcggggct cccgcggcga aagccccccg gcagcaaacg tcccgaaacc tcgacaccat
    14461 gggaaagggg ccgtttcccg acttcacggg aaacggcccc tcgacgtttt ggctccccag
    14521 cgcggactcg aaccacggac atgctggtta acagccagcc gctctaccga ctgagctact
    14581 ggggaacgtc tgtttcaggg cctcggatcg gccccttcgc ccaccgcaac gcaccggttc
    14641 gggcccggcc gtcgtctatc ggcagctcat gcaggtcttc agacggctca tgggcacgcc
    14701 cgtcctgacg ctgatgtatt tcaactgatc ctccggggcg aacgggcgac cgcagacctg
    14761 acaggtcgcc atcttgaagg ccttgtccca gatgatccgc ttgtctccgg tggtttccat
    14821 cttcacatga cccgtcggac agacgtaggc gcaggacccg cagccgatgc aggcattcgc
    14881 gtcgaaagcg atccgcggct cgtccacgtc gcgcccgagg ccccggtcct cgaaggacag
    14941 ggcggcgacc ccgacgatct cccggcaggt gcgcgcgcac aggccgcaga ggacgcattc
    15001 cttcgcgtcc gccgcctccg cggcgaccgc gatccccagc ttctccgcca gatcgcgcac
    15061 gacccgggtt ttggggtact tgtagtacag cagggaggcg gccagttttc ggcccgcgat
    15121 cgcccgctcg gagtccgtcc ggacctccat cccctgggca accggcgtgt tacaggccgt
    15181 ggcgagttcc catttgccgt tgaccttcac ctccacggtg cacaggcggc aggccccgaa
    15241 gggggacagt tcgtcgtgct gacagagcgt gggcaggtcg atgttgcgcg cccggatatg
    15301 ggagaggagg gtcgacccct cctgggcctt caccttccgt ccatctaacg tgatttcgat
    15361 catagcctgt tcctcgattg cgggtttctc ccccccggca acgctccgga cgggttcatt
    15421 gtcgtcaaga atccttcact ccgggaggcc gccaatcacg ggggccggcc ccttcccgga
    15481 tcctatgccc agaccatcag gcggtagcag cgcaggcagc gattggcctc tcggatcgcg
    15541 gccgcggcgt cgaagccgcc ctcgacctcg tcgaagcccg cgatgcgggt cttcgcctgc
    15601 aggagggccg cttccccgga cgccgccggg ctaacgaagc cctttgtgcg ctgggcacgc
    15661 gtgtcgatcc ccgcgaacgg gcgctccgcc gccggggttt cgccccgcag gtagaggtcg
    15721 atgctccgcg ccgcgcggtt gcccatgtcc agggcctcga tcagggtggc gggtccggtg
    15781 acgcagtcgc cgccggcgaa caccccgtcg aggttcgtcc caagcgtgac cgcgtcgacc
    15841 gccacggtat tgccctttgc caccgccagg gcctccgttc cggccaccgc cggcagtgcg
    15901 gggttctggc cgatggcggg gatgatcatc tccgtcttca cgctgaactc cgaccccttg
    15961 acggggacgg gacgacggcg gccgcttgca tcgggctcgc cgagtttcat cttgatgcat
    16021 tcgatcgccg tcaccttccc gttcttcgcg acgaccttca ggggggcggc gaggaagcgg
    16081 atcttcacgc cctcctcctg ggcctcggcg atctcctcgg cgcgggccgg catttcggcc
    16141 agcgaccggc ggtagaggat ctccacttcc ccgaacccca ggcggaggca ggtgcgggcg
    16201 cagtcgatgg ccacgttgcc gccgccgatg atgacgacct tccggcgcga ggcgatcttc
    16261 tgccccaggc tgatccgccg caggaactcg atcccttcca tgaagccgtc gtagccggcg
    16321 tcctcgccct cggcgccgat cgcgttgccc gcgtgcgccc cgacggcgat gaagacggcc
    16381 gcgtagccgg ccttcttgag cttccccggg tcgatgcggc ccaccggttc gttgaggcgg
    16441 atctccacgc ccaggcgccg gaccaggtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnt cgcggatcag gttcagggat tcgccaaagc gtccgttgcg aatcagctcg
    16621 atgtagccgg ggatatcgag ggtcgccgga caggcctcca tgcagggcgc cgtcacggcc
    16681 acgcggtagg tcttcgcggt tgtcttgccc gctgacgccg ctggattcgc gaaatccgcc
    16741 cggaaatatt tcagggcgtc gcccacgggg ttgagcgcgg ccgcgcggcc gaaattgcac
    16801 ttggcgttcg ccgcaacccc ccgaacgatc tcgtccatct cgtccaggtc cgccttggaa
    16861 cccttgccgg ccgcccgcgc gcgcagcagg tcgagcatgc ggtcgatgcc gatgaggcag
    16921 acggagcatt cgccgcagga gagctttctt gcctcctcga gataggccag cgtcagggaa
    16981 aggatgtcga cactcgggtc cgcgacgacg atcccgttcc agcccatgag ggcggtaacc
    17041 gcccccccgt cgatcgtcaa cggcgcttcg actttggcgg ccttggccgg tctgcccttc
    17101 cggttgtcga tcaccttgcc gccccaactg ctaaaggata ctgtcttcat gccttgttca
    17161 cctttgtttc ggatcggttt tgcggtcttc attccgtcca cgagagggaa tgttccacct
    17221 tgttcatgag cttccggtat tccaggttca cccggcgctg gatgtcccgg atctcgtcgg
    17281 gcgtcaggtg ccggaaccgg ccctgggcct tgatgtaatc cgtcacgggg cggagtggat
    17341 cgggcatctc cacggtcagg cgatacacgc cgttctccac ctcgtacagc gggaaaaccc
    17401 ccgtctccac ggcgagccga cccagcttga tgcactcctc cgacttggcc cgccagccgg
    17461 tggggcagac ggacaggcag tggatgtagg tcggcccctg gatcgaccgg gccttcttga
    17521 ccttgttcat gaagtcgagg gggtagctcg gcgaggccgt cgcgacgtag gggatgttgt
    17581 gggccaccat gatctcgggc acgtttttct tccaggtttt gttgccgatg ctggccttgc
    17641 ccgccggggc ggtggtcgtc gaggcgccga agggggttgc gctcgagcgc tggatccccg
    17701 tgttcatgta ggcctcgttg tcgaagcaga cgtagagcat gtcgtgaccc cgctccatgg
    17761 ctccggaaag ggcctggagg ccgatgtcca ccgttccgcc gtcgccgccg atggtcacgg
    17821 tcttgatgta gcggtcgggg ttcacgccct tgcgcctgag aaccttgagc cccgcctcca
    17881 cgccggcccc cacggccgcc gcgcagggaa aggcaacgtg gatccacggc agcgtccagg
    17941 cagtcgccgg atagggggtc gcgatcacct ccatgcagcc cgtggcgttg gcgatcacgg
    18001 tatccttgcc cagggccttg cacatgaggc ggatggccag ggcttcccca cacccctggc
    18061 aggcgcggtg tccggagcag aaaaactcct ccttgtccac cagcttcggg gcgtagatct
    18121 cgaagttctc cacgatcgat atcggttttg ccgtactcat catcccctca ctccatagaa
    18181 ttcatagatg cccttggact tgcccgcctg acggaacatc tgtttgaagt cgtccacggt
    18241 gacgtcgcgg ccgcccagcc cgatgagggc gttctcgatg accggccgtg cggcctccgc
    18301 gtacagggcg gaccggacct ccgccgccag cggacccccg ggaccgccca gggagcagca
    18361 ccggtcggtg acgatcaggg tcttgacgcc acgcaccgcc tttttcattt cctcgaaggg
    18421 aaagggccgc cagagcttga gcttcagaag ccccacggga acgccgtcct tgcggagctc
    18481 gtccacggca atctcggccg tctcgcccat ggagcccatg gtcatcatca ccacatcggc
    18541 cccctttgtc ttgtaggcct cgatgggttc gtaccgccgg ccgaactgtt tttcccattc
    18601 cttccagacc ttcaggatca ccttcctgga atcgaccagg gcctggtcgc gggccttggt
    18661 ggcctccgtg aagatgtccg gcatgcccag cgcgcccatc gtgacggggt ggtcggggtg
    18721 cagggccgca tagggtttgc gccgcggaag gaaccggtcc acctccttct ggtcgggaag
    18781 gaagagcgcc tcgaccatgt gcgtgagctg gaacccgtcc aggttaacgt tgatgggcag
    18841 ctgcaccgcc gcgtcctcgg cgatcttgaa ggactggatc atcatgtcca ccgcctcctg
    18901 gccgttggcg gcgaagaggg acagccagcc cagatcccgc atggtcatga tgtcgctgtg
    18961 atcgttccag atgttgatgg ggccggagat cgcccggttg ccgatcgcca tgacgacggg
    19021 cagccgcatg gacgaaacga tgcccaggac ctcgtacatg tagagcagcc cctgggagct
    19081 cgtcgccgtg aaggcccggg cccccgtgcc ggaggcgccg gcgcaggcgc tcatggcgga
    19141 gtgttcggat tcgacgcaga taaactccgc gtccagccgg ccttcggcca cgatatcgga
    19201 gaggtgctcg ggaatgtgcg tgttcggggt gatgggatag gccgcgatca cgtcgggccg
    19261 gcacagggcg accgcttccg cggcggcaag ggcgatttcc ataccaatgc gtttcgacat
    19321 gccttatccc tcctcaatca tggtgatcgc gcccggccag cattcgtggg cgcatatccc
    19381 gcagcccttg cagtaatcca ggtccgcggc gaagtagccc cgggcatcct ccttcacgca
    19441 tgcctccggg cagaagatgt agcagatgcc gcacttgatg cacttggcgt tgtcccagac
    19501 gggccgctgg gcccgccagc ttccggtatg gtactcccgg ctgttcccgc cttcaaagat
    19561 catgcagccc ggggtgactt cctgccaggt ttctggccat ttcttctgtg ccatgtggat
    19621 acctcgatct caataggact agtggatttt gacctcttcg taggcccgct gcagcgcctt
    19681 ctggttgcgg ggggcgatgc ggccgaaccg gtgctccagg ggagccgcca cggaagcgag
    19741 cttcaccacc ttcgtcgccc tgatcagggc ccccagcatg gtggtgttcg tgatgggcgt
    19801 accgagctcc tgcacggcga tcgcggaggc gtccaccatg gcgagccggc ccttgtagtt
    19861 gagggccttc cggatctccg ccgccgtgcg ggacgtattg acgacgagca ccccgtccgg
    19921 tttcaggccg tccgtcacgt tgacgaggcc gatcaggctc tcgtccagga ccacgaccac
    19981 gtccgggctg gcgatcacgc agcgccgccg gatccgcttc tcgtccaccc ggttgaaggc
    20041 cgcgaccggt gccccccgcc gttccggacc gaagctggga aatccctggg caaatcttcc
    20101 ctctccgatc gcggccaagg ccagcagttc gaccgatgtc accgctccct gaccgcctct
    20161 cccatgccat ctgacctcta acattggctt ctctccccga ttggaatcct gcaactcata
    20221 aacagaggcc ccatccttgg atctccccgc tagacgggaa ggggggtggg gtcgcctgta
    20281 tttccatcaa cgcattcatg gacatacccg acaaacaagg gttgttcgct attatattct
    20341 tccccggttg tcaacggata tttgcggtcc gggaaaccgg gtccggacgc gcggacattg
    20401 ccgaacgggt gcttgcgggt catccgcccg cggtgcgggc gaagagatct ccgccgcggg
    20461 catcgttgac gacgatgacg ggtaggtcga cgatctcgag gcgccgcacc gcctccggcc
    20521 ccagatcctc gtaggcgacg atctccgccc gctgcacgca ttttgccatc agggcggcga
    20581 tcccgccgat ggcgccgaaa tagacggcgc cgtgccgggc gatggcccgg acgacgtcct
    20641 cggagcggcg gcccttgccg atcatgccct tcagcccccg ctcgaggagg aggggcgtga
    20701 acggatccat gcggtagctc gtcgtggggc cgatggcgcc gatcggccgc cccggcggcg
    20761 ccggcgaagg cccggcgtag aagatcaccg ccccgtcgag atcaaacggc gggtcatcgc
    20821 cccgcgccag ggccgccgcg atgcgctggt gcgcggcgtc gcgggccgtg tagaccgcgc
    20881 cgccgatcag gagccggtcc ccgaccttca ggtcttcgca gatctccggc gcgagggggg
    20941 cggcgatgcg gcggggcagg tttttcatca acgcgttgaa attcaaggcc tgatcccctg
    21001 tcaaaggatg gcggtccggt gccgggccgc gtgacactgg aggttgacgg ccaccggcag
    21061 acacgcgatg tggcaggggc gcatctcgac gtggaccgcg agcgccgtca cctcgccccc
    21121 gtagccctgg ggtccgatgt cgaggcggtt gatctcttcg agcagcgcag cttccatcgc
    21181 cgccagacgc gcgtcggtgg ggtggggccg gccgaccggg cgcaggaggg cctgcttcgc
    21241 gaggaccggc gcctggtcga gcgatccgcc gatgcccacg ccgacgatca cgggcgggca
    21301 ggggttcggc ccggcctgcc gcacccggtc aagcacgtgc gcccggatgc ccgcctcgcc
    21361 cgcggcgggc acgagcatgg ccgcgctgct catgttttcg cttccgccgc ccttcggcag
    21421 ggccgtgatc cgcaggcggt cgccggggac gatggccgta tggagaacgg cgggggtgtt
    21481 gtcgcccgta ttgtggcggg tcaggggatc gcagaccgag gcccggagga acccgtcccg
    21541 gtaagcccgc cgcacgccct tctgaacggc cgtctcgaga tcgcccccca cgatatggag
    21601 gtcctggccg atttcgagaa agaccacggc cagtccggtg tcctggcaca ggggcatccc
    21661 ctcctcgtgg gccacccggg cgttttccag gatgcgctcc aggacatacc tccccaggcc
    21721 gttggtctcc cgctccccgg ccccttccag ggcggccagg acatcggccg ggaggaccgt
    21781 gttcgcggtc aggaacagct cccgcaccgt ttcggtgacg caccgggcgg aaaggtctcg
    21841 cgctgtcgtc atgaggggtc ccacctctcc gggggctaca ttccccggcg gaactcgatg
    21901 gaccgggcca gggtcatccg gtccgtgtac ttgaggtcgc cgcccacggg gacgccctgg
    21961 gcgatccgtg tgacggacac cccctgatcc ttcaccaggc gggtgatgag cagggccgtc
    22021 gcctccccct ggacgctggg attcgtggcg atgatgagtt cccggacgcc gttgccctga
    22081 acacgtttca ggagttcctg gaggcgcagc tgttcgggac cgaccccgtc gaggggtgcc
    22141 agcaccccgt ggagaacgtg gtaggtcccc cggaagctgc cgctctgttc gacggccatc
    22201 agggaatccg gctcctcgac cacgcagatc tgcccccggt cccgggagcc gtcgcgacag
    22261 atgtcgcact gttccccctc ggccagattg aagcagaccg ggcagaggtg gatcttctcc
    22321 ttcacctcga cgatgctctc ggcaagccgc cgggcgtcct cggccggggc ccggaggata
    22381 tgggtcgcca ggcgggtggc cgtccgctcg ccgatccccg gcaggcgcga cagctcccgg
    22441 atgagtttct gaatgggcag ggcataaccg gtcacggcat cccccttacg tcaacccggg
    22501 gatgttcagg ccgcccgtga gtttcatcat ctccgactgg gccatctcct gggccttgcg
    22561 gacgccctcg ttgaccgccg ccaggatcag gtcctggagc atctccacgt cctcggcatt
    22621 gaccacgtcc cgttcgatct tgagggagac gagttcgccc tttccgctga ccgtcacggt
    22681 caccatcccg ccgccggacg tggcggtgac ggtcttctgt tccatctccg cctggagttg
    22741 gcccatgcgc tcctggagct tcttggcctg tttcatgatg ttaccgatgt tgtgtgacaa
    22801 tgaaccgacc tccttagctg tcttgacgtc gtgcgcgccg cgttcatccc tccggattgc
    22861 ggatgatgac ctcctggatc atggcccccg gaaagacgtc cagggccttc ttcacgagcg
    22921 ggtggtcgag cgcctgccgt ttcaggtcgc cgttctttcc gttccgtgcg gcaccgttgc
    22981 cgttcgccgc cccggcaggg ccgttcgcga cgacctcgat ccgcacccgc agggaatcct
    23041 gccggaagaa ggtgcgggcg atctcctcca gccgcttctt ctccccgggc cccaggagga
    23101 acgcgtactc cttcggcatg gcgattttga gttcgttccc gccccggtcg aggatccggc
    23161 ccttctcgat cttgctgccc aggggaaaac tcgtctgtcg gacgaaggcc ttgaaccctt
    23221 cccagtcgtc tccggcggga tccggggcgg acgggtcttc ccggcgctcc ggtccggccg
    23281 cggggtccac cggttcccgc accgcccgcg ccgcgggggg ctccggcccg tcataggccg
    23341 gggaggccgc gggccggcct tcccgggacg caggcggggc gcccgtcagg cggcgttcga
    23401 tgccctccat ccggtcgagg atcacgtcga tcggcaggag cggctcgaga taggccatcc
    23461 gcacgagggc cgcctccagg ttgatccggg gatcgcggct ccggcgcacg gcctcttcct
    23521 cggcgagcag gatgtcaagc aggcgctgca gggtctcccg ggaaacctcg ccggccaggg
    23581 ccttcaggtg cgccagatcc gcgtcggaga cctcgatcaa acccggggcg tcgcccgcga
    23641 tcttcgtcat caggaggttc cggaaatgga cgaggagcat ctggtagaac accttcatgt
    23701 ccaggccggc gaagtatccc tcctcgacga tcttcagaca gcgggccgca tcgcgggcga
    23761 cgatcgcccg cgagagatcg aggacgaagc gacggtcggt caggccgagc agcgtctcga
    23821 cgtccccgtc ggcgatggtc gcccccccgt aggaaatgac ctggtcgaaa acgctctggg
    23881 cgtcccggag gcttccgtct cccgcttccg cgatccaggc gagacccgta tcgctgatcg
    23941 cgatcccctc cgccgccgcg atcgccttca ggctggacgc gatcgccttc agggaaatcc
    24001 gccggaaatc gaagcactgg cagcgggaca ggatggtggc cggcaccttg tgggtttccg
    24061 tcgtggcgaa catgaagatt acgtggggcg gaggctcctc cagggtcttc aggagggcgt
    24121 tgaaggcctc ccgggtgagc atgtggacct cgtcgatgat gtagatcttg taacggcagg
    24181 acgcggggga gaaccggacg ttctccctga gctcccggat ttcgtcgatc ccccggttgg
    24241 aggccccgtc gatttcgcgg acgtcgaggg cgatgccgtc ggtgatctct ttgcagttga
    24301 cgcaggcgtt gcagggggtg tccgtcgggc cggtctcgca gttcaaggcc ttggccatga
    24361 tgcgggcgac cgaggtcttc ccgaccccgc gcggaccgct gaagataaag gcgtgggcga
    24421 tccggcggtg gagaatggcg ttcttcagcg tctggacgac gtgctcctgc ccgaggacgt
    24481 cctcgaagat ctgcggccgc cattttcggg ccaggacccg gtattccgca ctcatcgttt
    24541 ccccgttctg cttggattaa agacggccgg gttacccgcg gcacacaacg gggattgtta
    24601 ccgctgcttc cttccggacc tgacggggtt cacaacccgc tgttgcacgg gacccggcca
    24661 tcaaacggcg acagcgcccg gcgggccgct gtcgatatgt ctggcggaga gagcgggatt
    24721 cgaacccgcg gtacaccttt gtgggcatac acacgatttc cagtcgtgc
//
LOCUS       DAOOLJ010000023        24478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_1932_length_24478_cov_14.645498, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000023 DAOOLJ010000000
VERSION     DAOOLJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 24478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24478
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_24478_cov_14.645498"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLW16_05675"
     CDS             complement(<1..290)
                     /locus_tag="PLW16_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPB70430.1"
                     /translation="MDTIQAVRGFKDILPPETGKWQFIERLAREVFADFGFQEIRVPI
                     LEKTELFRRGIGEATDIVEKEMYTFLDRGDEYLTLRPEATASILRAYLEHTL"
     gene            complement(373..2919)
                     /locus_tag="PLW16_05680"
     CDS             complement(373..2919)
                     /locus_tag="PLW16_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPB70431.1"
                     /translation="MQRPLIPICLALSAGIVAGHYHPVLAPAIPLTVLITLLGLLLAF
                     GRHPAGRSIIPGLLTVSFFTVGFLNANLNLAFPDSPTHIANSAAHEKETIEAMICDAP
                     RISPDGMDLVLCAHTILRPGARQAVAGRILLSVGVPDRRFRYGDLVRFRAIVRTPRNF
                     GNPGGFDYVRYLRDQGVRVRAYLPDSSGLVVMRRDQGQLFKTTLETFRARLKTFIYAH
                     AASPEAEIMQALILGDQEEIPERVREAFNRTGTTHILAISGFNIGIVALVFVFLVRMA
                     MKALPNALLWVDSRKASILMAIIPVLIFTLIAGAGISVVRATLMVVVFMIAILLDKER
                     DLYNALALAAFIILMIRPSSLYDISFQLSFSAVLAILYLTPRLISQIPKPPPPVNAES
                     ALADRARRWMHSRLYAAALFVAATLGATLGTLPLVLYHFHRISLVTLPANLLATPILG
                     ILSIPLSTALIFLVPIAPTLSAWILKVDALLVRLSVTLIEFCASFPHASVYLFRPSLV
                     EILLYYACLVLAASILPGSRESRAFPSPPRARPFSRSRRAVVLVILLIVWAAFSVSLT
                     LQPLRSNLLRLTAIDVGQGSATLVRFPFGKTMLVDGGGFHDQRFDVGKFVTAPYLWHE
                     RVYDLDICVLTHPHHDHLAGLLFVVRHFPIGEVWVADIPGSDLSLPLYREFLRIVREK
                     NIPLRRVSSETLPRTINGVTLATMNPTSISPSTGTRDDEMNDHSLVMKLTYGANAFLL
                     PADISGPAEAGLLDRRTNLASDVLFVPHHGGRHSSTEAFLLGVRPHRAVVSCGWENRF
                     GNPHPDILERYARLGIRLYRTDLDGAVEFSSDGRRIETYAPLSCRDPSRH"
     gene            3020..4693
                     /locus_tag="PLW16_05685"
     CDS             3020..4693
                     /locus_tag="PLW16_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB70432.1"
                     /translation="MAVDERLVQRVRWLMFWRLFIVTFLLGFVALIDVRDTTFLPYIS
                     RTFLYAIIILTYLLTIGFILLLKLTRAPILNVYVQSMADVLLITSLVYITGGIRSVHA
                     AFYPLVIIYSVLFLMRRGGLITASVCSLLYGLLLVSEHFGFIVPLSPSAYADHAFTAG
                     YVFTRILIYSASFFIIVFLASFVVEQERKAQTLLAETESAFNQLDILHQSIIDSVDAG
                     ILTMDVNGLVKSFNRGAERITGFVAREVVNRPLGHLFPDFVDVLARHSDPDSGASERR
                     PQFSFVSKEGGTLLIGCSVSTLNDPGGQRIGDILIFQDLTDVRAMQLAVEENKRLALV
                     GEMAAGLAHEIRNPLASITGCIQVLKRDLALKDEDEKLMQIILRGKEQLEGFLKDFLL
                     LSRAPAVRRDRIALDVLIRDVVESVRFLPDWHDRIRLSLKVASGDAFEASATEIRQVI
                     WNLILNAVQAMPEGGELRIEIVRHPLESKEKSYLELIVADEGEGIEEQLHNRIFTPFY
                     TTKARGTGLGLAIVQRVVMAHGGRIEVKSRPRQGTAFHIFLPCGDLGSN"
     gene            4708..6081
                     /locus_tag="PLW16_05690"
     CDS             4708..6081
                     /locus_tag="PLW16_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB70433.1"
                     /translation="MPKILVLDDDQNIRDMLVIMLTREGYDAQAYGDPYKAIQGLRKE
                     PFDLIITDLKMPRMDGLEFLRRAKKIAPDTVVILITAYASGETAVAAMKEGAFDYIEK
                     DFRIEDLTKIIERALDARSECSEDALFRQGVADAVSFGAMIGSSPEMVKIYTVINKIA
                     DTPVNVLILGESGTGKELVAKAIHENSSRKGMPFTVINCGGIPESLLESELFGYMKGA
                     FTGAHADKPGLFEVARGGTIFLDEIADLPPLLQVKLLRVTQEKTFRRVGGTEDIRVDV
                     RIISATNKDLQQRVYEERFREDLYYRLNVIPIHIPPLRERKDDIPILTRYFIEKYSRQ
                     FGKKIQNISTYALELLVDYPFPGNIRELENIIERSIALEASSIILPENLVLSNSASRF
                     EAPGDGIDIPPEGIHLNQELARVEKAYIDAALGKTNGSRKMAAELLNITLDSLHYRID
                     KLSDKSS"
     gene            6347..7558
                     /locus_tag="PLW16_05695"
     CDS             6347..7558
                     /locus_tag="PLW16_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB70434.1"
                     /translation="MKAIGKGFMMITGMMLALVAGAGETPAAQNIRVGAAINMTGPAS
                     TFGQYHAKGMKDYFRYVNEVRKGVDGRQIEMLLVDTGYKVPEAVAAVRKFAVQERVDM
                     MATWGAGEGLAAKPIVQRYRIPTINFSTSWEILQPPVSYMFLPFGSYRMDCQAVLEYI
                     RAVHKGKEAPRVGLLTYNNAYGRSIHQPTREYAAQNGINLVAVEEFPPKTMDLTTEML
                     RLKKKNVEYVFMQVLPATVVTALKNADRTAYKPLFLGTWTSTDPDFFPMAKGLIRDRL
                     VIQFPGGVPSDRSKGVKVLQALWKRYGTVKEFDASYWEGVVVGMIMERAFIRAKEKYG
                     RIDASTINRAMEGFRNEEFGGLVPPVTYTATEHGASFTARMVRVSENGEFVPLTNFYV
                     PGKEKIRILKK"
     gene            7570..8394
                     /locus_tag="PLW16_05700"
     CDS             7570..8394
                     /locus_tag="PLW16_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70435.1"
                     /translation="MKAYRGQPRIFARRDPASSRAELDVSGLTLQFGGVTALNDVGLH
                     VDTGELVAVIGPNGAGKTSLMNCITGFYRPQRGRIRFNGRDTTHLPPHLLTGIGIGRT
                     FQNIELFPGMTVLANMMLARHMHCRYRLMPAAFFTSVVRREEVRQREILEEVIDFLEM
                     QAIRNMPVGSLPYGMMKRVELGRALALEPRLLVLDEPFAGMNLEEKEDMVRFLLELHR
                     AWGQTMLLVEHDMSIVMGIAQKVAVLDFGQKLAEGTPEDIQRHPDVIKAYLGGDAS"
     gene            8391..10349
                     /locus_tag="PLW16_05705"
     CDS             8391..10349
                     /locus_tag="PLW16_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70436.1"
                     /translation="MSVEPMRQFHSSRRGDPPSSDAGVDTLPKMLARQASTLDASRIA
                     IREKAYGIWQTLTWPEYESYVRRCALGLISLGQRPGEINGLIIDNGPEWLFGELGTHA
                     AGGISLCLFTAAVAEELAGALKRVSARFVIVQDQEQVDKLLEVRERLPEIRHVIYVDP
                     TGMRPYREDPWLISFADLISRGKRLERERPELYNALLKKGRATGTAVMILTSGTTGLP
                     KIAMLSHGNLLAMGTKWRDALQIRPGENWYSMSPPAWIVDQMWGMGVALVSGMTMNFA
                     NTPETLLEDFREIGPHLLITSSRFWEDTASRIRVRMGDAGWLKRTLFAWAEGIGRKVL
                     GAEERKTAPPFGLRLRKRIARRIAFQPLLDRIGCSQLRSAFTGGHPISPDVIRFFRAT
                     GLNLKQCYGLTEAGGIFQVQPDGEVKPETVGKPLPDTQVMIGEDQEVMVKSDANFIGY
                     YQDFAATEAAFREGWLRTGDAGYLDEDGHLVIIGRREEIIRNKEGQAFSPDYIETRLK
                     FSPYIKEAVVFGEGKPFLTALVNIDFGNVGAWAEERMIPYTTYNDLSQQADVAALLRE
                     ELRRTNERLPQAMRIRKFILLYKLLDADDEELTRTGKVRRRFVYGLYLPLIEAMYGDL
                     TTLPVKGKVKYRDGQVGVIETCVRILEA"
     gene            10366..11244
                     /locus_tag="PLW16_05710"
     CDS             10366..11244
                     /locus_tag="PLW16_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB70437.1"
                     /translation="MDFFIQLLVNGLSVGFLYGLSAMGFVMIFKSSSVLNFAHGELLA
                     LGAFVFLSLHAVVQLPIAWAFLATLTGCFVLGFLIERFFLRPLIGEPLIYVIMLTVGL
                     AAMLRGLLLLIWGGNLRTLPDFLPAWMDIQAGWLRIPPVYMAAILVGILFLVLFGLFF
                     KYSAQGIFMRSVADNQRAALSLGVHVRRVFALSWGIAALVAGMSGIILGLMNGISVHD
                     LSAVGLKVFPVVILGGLNSITGAVIGGLIIGVLEAFTGGYLSPSLRDVVPYIALVVLL
                     LVRPYGLFGLVEIERV"
     gene            11254..12324
                     /locus_tag="PLW16_05715"
     CDS             11254..12324
                     /locus_tag="PLW16_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB70438.1"
                     /translation="MRKHHLHPCGNYKESYTDELAIFSSDYGRLWSMAGLAALFCAVP
                     FISPPYLLYVINMIGIYAIAAIGLNILVGYTGMISLGHGAFFGVGAYAAAVMSATFNV
                     PFLLSVPAAGLLTAGVGMIFGIPSGRLKGLYLTIATLAGQFIIEYVLVHWEGVTRGTE
                     GITVPAPTLFGISLRGDISFFYVIFSVLTLMIWVAVNLIRTRYGRAFIAIRDNDRAAE
                     GMGIPIFSYKILSFGISSFYAGLAGALWAYYMVSVTSEPFNLSLSVEYIAMVIIGGLG
                     SIAGSIYGAVFITVLNESLRWLTDLMMNFGFITESGLNMAPLREFAFGLAIVLFILFE
                     PRGLAELWRIIRSSFRLWPFSY"
     gene            12335..13132
                     /locus_tag="PLW16_05720"
     CDS             12335..13132
                     /locus_tag="PLW16_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70439.1"
                     /translation="MEDTLLEINNISVVYADVIQVLKGVSLQVPKRRIVSLLGSNGAG
                     KTTTLKAVSGLLKPENGRVTDGAILYEGHPLENRSPEQITRLGIIQVLEGRQPFKYLT
                     IEENLRVGTATRWGKPYRKDLELVYHYFPVLWERRKALAGYCSGGELQMLVMGRALMA
                     HPKLLLLDEPSLGLAPLVVEEIFRIIARINGEQQTTIILVEQNARIALEIAHYGYVME
                     NGKIVMEGTSETLKDNPDVKEFYLGGGAGGPGAYQQPKSYRRRKRWL"
     gene            13172..14431
                     /locus_tag="PLW16_05725"
     CDS             13172..14431
                     /locus_tag="PLW16_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70440.1"
                     /translation="MAEGFDAWEHENAEVRRTRKFSRMCAMLEAGYGASSRLRRILDE
                     TGLMPADFSRDLDLERLPVISRETLIQWELSEPPYGGLADPAADVDRIFISPGPVYEP
                     HLSEADMLWARAYHAAGIGKADRVLNAFSYHLVAAGLTFHGGLRRVGATVVPSGTAGA
                     QNQVELIRDLGITAYTGTPSFLMAIIRKAEEMGYDWEKDFRLKRACFAAEPLRPALRE
                     TFTRDYGIDTYQMYGATEVGDVAYECRQKSGWHICEEVFVEICDPESGRRLPAGELGQ
                     VVVTRFNDIFFLMRFGTGDLSRLIETPCDCGRTAYRLDGIAGRVGEAVKVRGMFVAPS
                     QIHRLAQRFSGKIFRVHVRREGDRDLLVVRIEGAPGTQGDGWAGDEEFKRGFRDLCTV
                     GVDRLEWVPKGTLAAADRQLVDERDWR"
     gene            complement(14477..14845)
                     /locus_tag="PLW16_05730"
     CDS             complement(14477..14845)
                     /locus_tag="PLW16_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70441.1"
                     /translation="MIRYIRDHEQRVRTALAGAATDDAWPELRALHQSRLTYLQHERL
                     AHLHVTLAVGLYALLFFLASLFAPTALLWTVTALFVVLEGAYLIHYYHLENSVQRWYR
                     LADEIAEHLPARTTGTQPVD"
     gene            complement(15179..16927)
                     /gene="recJ"
                     /locus_tag="PLW16_05735"
     CDS             complement(15179..16927)
                     /gene="recJ"
                     /locus_tag="PLW16_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPB70442.1"
                     /translation="MVHTLPTTRWKIPEGNRPLQESLIRELGIHDVLSQILINRDIQD
                     PDTARRYLRPSLRDLHSPFLMKDMPEGVRRLARALHNRERVMIYGDYDADGITSVVVL
                     YKFLKGIDADVLYAIPDRIKEGYGLHLRAVDDMAARGIRLIVTVDCGISDHEAIAHAR
                     SLGIDTIILDHHEVPPSLPPAVASINPNRADCTFPFKSLAAVGIVFNFLIALRGQLRQ
                     EGFWTNRPYPNLRDYLDLVALGTIGDIAPLIDENRIFARIGLDLVTEGRRVGLVALKE
                     ICSLDHQAIDSSKASYGLIPRINAAGRIASPEEAIRLLLTEDEEEARQCAALLDGFNR
                     KRQALEKGILQELIAGIEKDPDSGRRSSFVFASERWHPGVIGIVAARLVDRYGRPAIL
                     ISLKDGVGKGSGRSVPDFNIYEGLKRCASALLSYGGHRFAAGISIRSADVGSFARQFD
                     AIVAEDLSGASFMTQTTIDAQCELPEINHELLSQLGLMSPFGSMNPEPVVCIRGVNVQ
                     TPTIVGNNHLRMRISSNGASCSSIWFNKGHLLPAVAGSTLDIAFTPQYNHWNGSSVIQ
                     LKMKDMAIPDADPAYP"
     gene            17141..17557
                     /locus_tag="PLW16_05740"
     CDS             17141..17557
                     /locus_tag="PLW16_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70443.1"
                     /translation="MSRRRLLWIVMLVLPLVLGMGSRSNDSPEKIPAPMKKFRASFID
                     QMDVLTQCTNVSIDGETYLQGKRGEGNYTIGFDRIASVVLHLSGGKLIGVVKLRDGTS
                     LELILNGSQKAYGFTGYGTFQIRLQDLKKMTIGGKG"
     gene            complement(17593..17790)
                     /gene="rpmB"
                     /locus_tag="PLW16_05745"
     CDS             complement(17593..17790)
                     /gene="rpmB"
                     /locus_tag="PLW16_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPB70444.1"
                     /translation="MSRMCEVCGKGPTFGHNVSHANNKTKKVWYPNLQRMRCIDQKTG
                     AIKRVRVCTRCLRSGFVKKAV"
     gene            complement(17896..19209)
                     /locus_tag="PLW16_05750"
     CDS             complement(17896..19209)
                     /locus_tag="PLW16_05750"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPB70445.1"
                     /translation="MRNHLPYDFTIQSLGECTTDSPLNVSFFTSDSKRVLFDHFLPDK
                     DDLRKEPDAAPLSVELAGPRDKIFFEPIQTKAAIVTCGGLCPGINDVIRAIVMELYHR
                     YEVRNIIGIRYGFRGLIPRFGYELMELHPDTVENIHGVGGSLLSSSRGRQDIVEMVNA
                     LKRMNIDILFCIGGDGTMRAAEALTDEITRRKLNISVIGIPKTIDNDLTLIQKTFGYD
                     TAISEAVNAIRCAHVEAKGAPMGIGLVKIMGRQSGQIAAGSALAQNDVNFVLVPEVQF
                     DLEGENGFLAALEKRLRHRHHAVILVAEGAGQEHIEPGQGTVETDASGNPRLKDIGFF
                     LKTRIEEHFSRLNIEVNLKYIDPSYMIRSVTANASDSIYCSALGQYAVHAGMAGKTGM
                     LVGLMQDEYVHLPLKMVSSKAKLDPQGMIWMRVLESTGQPPVLKN"
     gene            complement(19213..19905)
                     /gene="purN"
                     /locus_tag="PLW16_05755"
     CDS             complement(19213..19905)
                     /gene="purN"
                     /locus_tag="PLW16_05755"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPB70446.1"
                     /translation="MKKRIVTLGVLASGNGSNLQAIIDRIEARALDARIAVVISDNPR
                     AFALERAARHGIPVVKVPSGSHPSREAADAAMIQVLNEYGVELVVLAGFMRLLSETFL
                     RTFPMRVINIHPALLPAFPGVHSQRQAANYGVKFSGCTVHFVDQGVDTGPIIVQSVIP
                     VEEADTEATLGARILEQEHRIFPQAIQWYAEGRLRVEGRKVFLGAGRTCGRVALHQPA
                     LESGPGDFGKEE"
     gene            complement(19939..20991)
                     /gene="purM"
                     /locus_tag="PLW16_05760"
     CDS             complement(19939..20991)
                     /gene="purM"
                     /locus_tag="PLW16_05760"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPB70447.1"
                     /translation="MSDNASYKTAGVDIDEANRFVERLKPLVKTTFRKEVMSGIGGFG
                     GLFHLDPEKYRDPVLVSSTDGVGTKLKIAQEMMRHDTIGIDLVAMSVNDIVVQGAEPL
                     FFLDYLSLGKIDVGRCLEILDGIVRGCQDAGCALIGGETAEMPGFYRDDEYDLAGFCV
                     GIVDADRLLDGSGIRVGDRIIGLASSGLHSNGFSLARHVLFERGGYRLGDRLEEFDNT
                     IGDELLTPTRIYVRTVLNLIKNFTVKGIVHITGGGFIDNIPRIIPAASRVIIQMGAWP
                     IHPVFSAIQEIGNIVDDEMYRVFNMGIGMMLVVPEKESGEILERLEQMGEKAYLIGQI
                     DKRDSAQPSVVLLPPA"
     gene            21163..23361
                     /gene="recG"
                     /locus_tag="PLW16_05765"
     CDS             21163..23361
                     /gene="recG"
                     /locus_tag="PLW16_05765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPB70448.1"
                     /translation="MSDLPNRLQESIPLPSPQPELEGGPPGAVLTRRFARLDQPVRFL
                     KGVGPKMAERLARKDLNTIEDLLYFLPRRYEDRRNLKPLAQIEAGRRETVVGTVVRSG
                     LTSRGYRHQFEVTFKDDGGTMTARWIRGRFAYLHGIFRVGAMFILTGDVRSTYSGPEM
                     IHPDFEFLDGEDERDRPPFRRIVPVYSETEGLPQKTIRRIMGGLLETHAEDLVSPIPA
                     SVCRRQRLMDMPEAVRRVHCPDLGEEMDDLNGMRSASRRRIVFDELFFFQLGMALRYR
                     GNVMEPGCPLKTRGPMLKRFFEALPFRMTPAQERVWSEIAHDLDRPSPMNRLLQGDVG
                     SGKTVVALAALITACQNGFQAALMAPTEVLAAQHHRTIRHWLQPLGIEAELLTGSLTV
                     RERQSVYGGMQRGAIQVAVGTQALIQASVSFQRLGLIVIDEQHRFGVLQRGSLRGKGR
                     HPHVLVMTATPIPRTLAMTLYGDLDVSIIDGLPPGKKSILTRVLTEGQRSSAYRAVRQ
                     EVEQGRQVFIVYPLVEESEVLDLKDATRMAEHLKADVFPNCVVGLIHGRMAGTEKDIV
                     MERFAKGEIQILVSTSVIEVGIDVPRASLMVIEHAERFGLSQLHQLRGRVGRGEIASA
                     CLLMVGKGVTLTARRRLRIMAESNDGFRIAEEDLAIRGPGEFMGTRQSGIPDFRVAHL
                     LRDMSVLQAAKEEAFRVVSEDPTLTHPRNEALRQVLFRRWDGRLHLARTA"
     gene            23433..>24478
                     /locus_tag="PLW16_05770"
     CDS             23433..>24478
                     /locus_tag="PLW16_05770"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPB70449.1"
                     /translation="MRTIGLGLMGFGTVGTGVVKLLQENRDVLEARLGACLTLKKIAD
                     LDIVSPRPVAVDPGLLTRRAEDLIEDPDIDIVIELMGGFDPARTLILKAFASGKHVVT
                     ANKALLATHGNDIFCEAEARGVNIGFEASVGGTIPVIKTLREALGANHIRSIFAIMNG
                     TSNFILSRMTDEGESFDTVLKRAQVLGHAEADPTYDVEGIDTAHKLAIVLSLAYGRRV
                     RLEDIYREGISHLTPQDIELARELGYRIKLLAIALEREDGIEARIHPTMIPFKHLLAS
                     VNSNFNAFHIIGDAADSVFLYGQGSGMMPTASAVVSDLMDIARDILKGISCRVPSRAL
                     HEASMPDIVILPMA"
BASE COUNT         4913 a         7061 c         7670 g         4834 t
ORIGIN      
        1 tagagggtgt gctcgagata ggcgcggaga atcgacgcgg tcgcttccgg cctcaaggtc
       61 aggtattcgt cgccgcgatc gagaaacgtg tacatctcct tttcgacgat gtcggtggct
      121 tccccgatcc cgcgtcggaa gagttccgtt ttttccagga tgggaacacg gatctcctga
      181 aagccgaaat ctgcgaaaac ctcccgggca agtcgctcga tgaactgcca tttgccggtc
      241 tccggaggga gaatgtcctt gaagccgcga accgcctgaa tcgtatccat gattctctcc
      301 cgagggtcac cttggaggca cccttttgga acgcggttca ctaacacatc gggtttgaaa
      361 aggcaatcaa aatcaatgcc gtgaaggatc tcgacaggaa aggggggcgt acgtttcgat
      421 gcggcgtccg tcggaggaga attccaccgc gccgtccaga tccgtgcgat ataggcggat
      481 ccctaggcgg gcataccgtt ccaggatatc cggatgcgga tttccgaagc gattttccca
      541 gccgcaacta acgacggccc ggtgaggcct cacccccagc agaaaggcct ccgtgctgga
      601 gtgcctgccg ccatggtggg ggacgaacag gacatcgctc gcgagattgg tgcgacggtc
      661 caggagcccc gcctcggcgg gtccggaaat gtccgccggc agaaggaacg cattcgcccc
      721 ataggtcagt ttcatcacga gggagtggtc gttcatctcg tcgtcccgcg tcccggtgga
      781 aggtgagatc gaagtcgggt tcatggtggc gagggtaaca ccgttgatcg tgcgggggag
      841 cgtttcggag gaaacccgtc gaagggggat gttcttttcg cggacgatcc gaagaaactc
      901 ccgataaaga ggcaaggaaa gatcggaacc cgggatatca gcgacccaga cctcgcctat
      961 gggaaaatgc cgcacgacga agaggagacc ggccaggtgg tcgtgatgcg ggtgcgtgag
     1021 gacgcagata tcgaggtcat aaacgcgttc atgccagaga tacggcgcgg tgacaaactt
     1081 tccgacatcg aacctctgat catggaatcc gccgccgtcc accagcatcg tcttgccgaa
     1141 ggggaagcgc accagggtcg cactgccctg accgacatcg atcgcggtaa ggcgcaggag
     1201 attcgatcgg aggggttgca gggtgaggga gacggaaaag gccgcccaca cgatcagcag
     1261 gatcacgaga acgacggcgc gacgagaccg gctgaaaggg cgcgcgcggg gcggggaagg
     1321 gaacgcacgg gattcccgcg agccgggaag gatcgaggcg gccaggacca ggcaggcata
     1381 gtagaggagg atctcgacga ggctcggcct gaacaggtat accgaggcat gcggaaagga
     1441 ggcacagaac tcgatcaggg taacggacag acggaccaga agggcgtcga ccttcaagat
     1501 ccaggcggag agggtcggtg cgatcgggac cagaaagatc agcgccgtgc tgagcggaat
     1561 cgagaggatc cccaggatgg gggtagcaag aagattggcg ggcagcgtga cgagggagat
     1621 gcggtgaaag tgatagagaa ccaggggaag ggtccccagc gttgcgccca aggttgcggc
     1681 gacgaacagg gcggcggcgt acagccgaga atgcatccag cgtcgggccc ggtccgcgag
     1741 agccgactcc gcgtttacgg gcgggggcgg tttcgggatc tgggagatca atcggggcgt
     1801 gagataaaga atggcgagga cggccgaaaa ggacaactga aaggagatgt cgtagaggga
     1861 ggaggggcgg atcatcaaaa tgatgaaggc ggcgagtgcc agtgcgttgt acagatcccg
     1921 ttccttgtcg agaaggatgg cgatcatgaa gacgacgacc atcagagtgg cgcgaaccac
     1981 ggatatcccg gcgccggcga tgagggtaaa gatcaggacg ggaataatcg ccatcaggat
     2041 ggaggccttt cgggagtcca cccaaagaag cgcattcgga agggctttca tggccatccg
     2101 gacgagaaag acaaaaacca gcgcgacgat gccgatgttg aaaccggaaa tggcgagaat
     2161 gtgggtcgtg ccagtgcgat tgaaggcctc gcggacccgt tcggggatct cttcctgatc
     2221 cccgagaatc aaggcctgca tgatctcggc ttcgggcgag gcggcatggg catagatgaa
     2281 ggttttcagg cgggcgcgaa acgtctccag ggtcgtcttg aaaagctgtc cctggtcccg
     2341 tcgcatgacg accaaaccgc tcgaatcggg gaggtaggcc cgcacccgga cgccctggtc
     2401 tctcagataa cggacataat cgaacccacc cggattcccg aagtttcgcg gcgtgcggac
     2461 gatcgcccgg aaacggacga gatccccgta gcggaaacgg cggtcgggga cgccgacgga
     2521 cagaaggatt cggcccgcga cagcttgccg ggccccaggc ctgaggatcg tatgggcgca
     2581 gaggacaaga tccatcccgt cgggagaaat ccgtggggcg tcacagatca tcgcttcaat
     2641 cgtttccttt tcatgagcgg cagagtttgc tatatgggta ggcgaatcgg gaaacgcgag
     2701 attcaaattc gcgttcagga acccgacggt gaaaaacgag acggtcagga gccctgggat
     2761 gatggaccgg ccggcaggat ggcggccgaa cgccaacagc aatcccaaca gggtgatcag
     2821 gacggtcagg ggaatcgccg gggccagaac gggatgataa tgcccggcga cgattccggc
     2881 cgaaagggcc agacaaatcg gaatgagagg cctttgcatg ggtcacctgc tgtgcgcttc
     2941 acacgggagg ccgcttcctg ttagggatat atcgtgaaat ccccggatct tcaacccaaa
     3001 accgtttcgg gtgcgccgta tggccgttga cgagagactg gtgcagcgcg tccggtggct
     3061 catgttctgg cggctgttca tcgtcacctt cctgctcggg ttcgtcgcgc tgatcgatgt
     3121 ccgggacacc accttcctgc cgtatatatc ccggacgttc ctctatgcca tcatcatcct
     3181 cacctatctg ctcacgattg gctttatcct gctgctcaag ctgacccggg cgccgattct
     3241 caacgtctac gttcagagca tggccgacgt gctgctgatc acctccctgg tttatatcac
     3301 gggaggtatc cgcagcgtcc acgccgcatt ttatcctctc gtcatcatct attcggttct
     3361 ctttctgatg cggcgaggcg gtttgattac agcctcggtc tgcagcctgc tgtacggact
     3421 gctcctcgtt tccgaacatt tcggatttat cgtgccgctg tccccgagtg cgtatgccga
     3481 ccatgccttc accgccgggt acgtctttac ccggatcctc atctattcgg cgtctttctt
     3541 catcatcgtc ttccttgcca gtttcgtggt ggagcaggaa aggaaggcgc aaacgctgct
     3601 ggccgaaacg gaaagcgcct tcaatcagct cgacatcctt catcagagca tcattgattc
     3661 cgtggacgcc ggaatcctga cgatggacgt aaacggtctc gtcaaatcct tcaaccgtgg
     3721 tgcggaacgg atcaccgggt ttgtggcgcg cgaggtcgtc aacagacccc tcgggcatct
     3781 atttcccgat ttcgtggacg tcctcgcgcg gcactcggac cctgattcag gcgcgtcgga
     3841 acggcggccg cagttctcct tcgtgtcgaa ggagggggga acgctcctca tcggctgctc
     3901 ggtctcgaca ctgaacgatc cgggaggaca gcgtatcggc gatatcctga tctttcagga
     3961 tctaacggac gtcagggcga tgcagctcgc cgttgaggag aacaagcgcc tggccctggt
     4021 cggtgagatg gctgcaggcc tcgcccatga aatccgcaac cccctcgctt cgattaccgg
     4081 atgcattcag gtcctgaaac gggatctcgc cctgaaggac gaggatgaaa aactcatgca
     4141 gattatcttg cggggcaagg aacagctgga gggatttctc aaggacttcc tgctgctctc
     4201 ccgcgcaccc gccgtgaggc gtgatagaat tgcgcttgac gtcctgattc gggatgtggt
     4261 ggaatccgtc cgctttctgc cggactggca tgatcggatc cggttgtccc tgaaggtggc
     4321 gtccggcgac gcgttcgagg ccagtgcgac ggagatccgc caggtaatct ggaatctcat
     4381 cctgaacgcc gttcaggcca tgccggaagg cggagagcta cggatcgaga ttgttcgtca
     4441 ccccttggaa agcaaggaga agagttacct ggagttgatc gtcgccgacg agggtgaagg
     4501 gatcgaagag cagctgcaca acaggatttt tacacccttc tacaccacca aggcgcgggg
     4561 caccggtttg gggctggcca tcgtccagcg ggtcgtgatg gcgcatggcg gacggatcga
     4621 ggtgaagagc cgcccacggc aggggaccgc ctttcatatt ttcctgccgt gcggcgatct
     4681 cgggagtaat tagcagaaag ggacgcaatg ccgaagattc ttgttttaga cgacgaccag
     4741 aacatccggg acatgctcgt gatcatgctg acgcgcgaag ggtacgacgc ccaggcttat
     4801 ggggatcctt acaaggcgat acagggcttg cgcaaggaac ccttcgacct catcatcacc
     4861 gacttgaaga tgccccgcat ggatggactg gagtttctgc ggcgggccaa gaaaatcgcg
     4921 cccgacacgg ttgtcatcct gatcaccgct tacgcttccg gcgagaccgc cgtcgcggcc
     4981 atgaaggagg gggctttcga ctatatcgaa aaggattttc gcatcgagga cttgacgaag
     5041 atcatcgagc gggcgctgga tgccaggagc gagtgcagcg aggacgccct gttccgccag
     5101 ggggttgccg atgccgtcag cttcggtgcc atgattggaa gcagcccgga gatggtcaag
     5161 atctacaccg tgatcaacaa gattgccgat acgcccgtga acgtcctgat tttaggtgaa
     5221 agcggaacgg gcaaggaact ggtcgcgaag gcaatccatg aaaacagctc gcggaagggc
     5281 atgcccttca cggtgatcaa ttgtggcggc atcccggaaa gccttctcga gagcgaactg
     5341 tttggataca tgaagggtgc gttcacggga gcccacgccg ataagcccgg gctgttcgaa
     5401 gtcgctcgcg gcggcacgat ctttctcgat gaaatcgccg acctgccacc gctgctccag
     5461 gtgaagctgt tgagggtgac gcaggaaaag acctttcgcc gcgtcggcgg cacggaggat
     5521 atccgggtcg atgtccgcat catctccgcg accaacaagg atctccaaca gcgggtttac
     5581 gaagaacggt tccgggagga tctctattac cgactgaacg tgattccgat ccatattccg
     5641 ccgctgcgcg agcgcaagga cgacatcccg atcctcacgc ggtacttcat cgagaagtat
     5701 tcccgtcaat tcggcaagaa gatccaaaat atctctacct atgccctgga gctcctcgtc
     5761 gattatccct ttcccggcaa catccgagaa ctggaaaaca tcatcgagcg ctccattgcc
     5821 ctggaagcct caagcatcat ccttccggaa aatctcgttt tgtcgaacag tgccagccgg
     5881 ttcgaggcgc cgggtgacgg gattgatatc cccccggagg gaatccatct caaccaggag
     5941 ctggcccgcg tggagaaggc ctatatcgat gcggcgctgg gaaaaaccaa cggatccagg
     6001 aaaatggcgg cggagctcct gaacataacg ctggactccc tgcattaccg gatcgacaag
     6061 ctgtccgata aatcatcatg aatgaatgtc aaaaatattt cttgacaaag gatcgtcgat
     6121 cctttagtca cagtcaacaa tgttttacgg ggagtgttgt catgaaggcc acagcgttcg
     6181 ggaaagccaa tccgtttttc gtctttttta gctttaggcg gaggtctgcc tgatgggctc
     6241 atgaccacat cgagggatct tgagccgtgg gcggaccatc tgaccgccca cggctttttt
     6301 ttgccgtggt gaacgccacg gtttttttta tccggaagga gggattatga aagcaatcgg
     6361 caagggattc atgatgatca ccggcatgat gctggcgctg gtggcgggag ccggcgagac
     6421 gccggccgcg cagaacatcc gggtcggtgc ggccatcaac atgaccggac ccgcctcgac
     6481 cttcggccag tatcacgcca aggggatgaa ggactatttc cgttacgtca acgaagtccg
     6541 aaaaggggtc gacggacgcc agatcgagat gctcctcgtc gatacgggct acaaggtccc
     6601 ggaggccgtg gccgccgtcc gcaagtttgc ggtacaggag cgcgtggaca tgatggcgac
     6661 gtggggggcg ggagaaggct tggccgcgaa gcccatcgtt cagcgctacc ggattcccac
     6721 catcaacttc tcgaccagct gggagatttt acagcctccc gtcagctaca tgttcctccc
     6781 cttcggaagc taccgtatgg attgccaggc ggtcctggaa tatatccgtg ccgttcacaa
     6841 ggggaaagag gccccgcggg tagggctgct cacctacaac aatgcctatg gacggtcaat
     6901 ccatcagccg acacgtgagt acgcggctca gaacggcatc aatctcgtcg ctgtcgagga
     6961 gtttccgccc aagaccatgg atctgacaac ggaaatgttg cggttgaaga aaaaaaacgt
     7021 ggaatacgtc tttatgcagg tcctgccggc cacggtcgtc accgctctga aaaacgccga
     7081 tcggaccgcc tataagcctc tgtttctggg aacctggacc tccaccgatc cggatttctt
     7141 cccgatggcc aaagggctga tccgggatcg cctggtgatt cagttccccg gcggtgttcc
     7201 gtccgaccgg tccaaggggg tcaaggtcct acaggctctc tggaagcgtt acgggaccgt
     7261 caaggaattc gacgcctcct attgggaggg tgtcgtggtg ggcatgatca tggagcgggc
     7321 gttcattcgg gccaaggaaa aatacggccg gatagatgcg tcgaccatca atcgcgccat
     7381 ggaaggcttc cgcaacgagg agttcggcgg cctggtgccc cccgtcacct ataccgcaac
     7441 ggaacatggc gcctccttca cggccaggat ggttcgggtc agtgaaaacg gggaattcgt
     7501 ccccctgacg aatttctacg ttccggggaa ggaaaagatc cggattctga agaagtaagg
     7561 acgtccgcga tgaaagccta ccggggtcaa ccccgcatct ttgctcgtcg ggatccggct
     7621 tcgagccgcg ccgagttgga cgtgagcgga ctcacgcttc aattcggcgg cgtaacggcg
     7681 ctcaacgatg tcggactgca cgtggatacc ggcgagctcg tcgccgtcat cggtccgaac
     7741 ggtgccggga agaccagcct gatgaactgc atcaccggat tttaccgacc gcagcgcgga
     7801 aggatccgct tcaacggacg cgacacgacc cacctgccgc cgcatctact gacggggatc
     7861 gggatcggcc gcacctttca gaacatcgag ctttttcccg gcatgaccgt tctcgcgaac
     7921 atgatgctgg ctcggcacat gcactgccgg tatcgcctga tgcccgccgc attctttacc
     7981 tccgtggtcc gacgcgagga ggtccgccag cgcgagatcc tcgaggaggt gatcgacttt
     8041 ctggagatgc aggcgatccg aaacatgccc gtcgggtccc tgccatacgg gatgatgaaa
     8101 cgggtggaat tgggccgcgc ccttgccctc gagccccgcc ttctggttct cgatgaaccg
     8161 tttgccggca tgaatctgga ggagaaggag gacatggttc ggtttctgct cgaacttcac
     8221 cgggcctggg gtcagaccat gctgctggtc gagcacgaca tgtccattgt catggggatc
     8281 gcccagaagg ttgccgtgct cgacttcggt cagaagctcg ccgagggaac gcctgaggac
     8341 atccagcgcc atccggacgt gatcaaggcc tatctggggg gagacgcatc atgagcgtgg
     8401 aaccaatgag gcagttccac tcgtcgcgac ggggcgatcc tccttcatcc gatgccggcg
     8461 tcgatacgct gcccaaaatg ctcgcccgcc aggcatcaac tctggacgca agccggatcg
     8521 cgattcggga aaaggcctac ggcatctggc agaccttgac ctggcccgag tatgaatcct
     8581 atgtcaggcg gtgcgccttg gggctcatat ctctcgggca acgccccggc gagatcaacg
     8641 gtctcatcat cgacaacggc ccggaatggc tgttcggcga actcgggacg cacgcggcgg
     8701 gggggatctc tctgtgtctg tttaccgcgg ccgtggccga agagctcgcc ggcgccctga
     8761 agcgcgtgtc cgcgcgtttc gtgatcgtcc aggatcagga gcaggtggac aagcttctgg
     8821 aggttcgcga acgcctgcct gaaatccggc acgtcatcta tgtggatccg accgggatgc
     8881 ggccctatcg ggaagacccc tggctcatca gtttcgctga cttgatctcg cgcggcaagc
     8941 ggctggagag ggagcggccg gagctgtaca acgcgctctt gaaaaagggt agggcaaccg
     9001 ggacggcggt catgatcctc acctccggaa cgaccggact gccgaagatc gccatgctgt
     9061 cccacggcaa tcttctggca atggggacaa aatggcgaga tgccctgcaa atccggccgg
     9121 gggagaactg gtattccatg tcccctccgg cctggatcgt cgatcagatg tgggggatgg
     9181 gggtggctct cgtcagcggg atgaccatga attttgctaa cacgccagaa actcttctgg
     9241 aggatttcag ggaaatcggt ccccatctgc tcatcacctc gtcccgattc tgggaggata
     9301 cggcctcgcg catccgcgtc cggatgggcg atgcgggctg gctgaagcga accctgtttg
     9361 cctgggcgga ggggatcggg cgaaaggtcc tgggagcgga ggaacggaaa acggcgcccc
     9421 ccttcggtct ccgcttgcgg aagcggatcg cccgccggat cgcgttccag cccctgctag
     9481 accgcatcgg ttgctcgcaa ctccgctcgg ccttcaccgg agggcatccc atcagcccgg
     9541 acgtcatccg tttcttccgc gccacggggc tcaatctcaa gcaatgctac gggctgaccg
     9601 aggcgggagg tatattccag gttcagccgg acggagaggt gaagccggaa acggtgggga
     9661 agccgcttcc ggacacccag gtcatgatcg gtgaagacca ggaggtgatg gtcaagagcg
     9721 atgccaattt cattggctac tatcaggact tcgccgccac ggaggcggcc ttccgcgagg
     9781 gatggctcag gaccggggac gccggttatc tggatgaaga cgggcatctt gtgatcatcg
     9841 gccgccggga agagatcatc cgcaacaagg agggtcaggc cttttccccg gattacatcg
     9901 aaacgcgact gaagtttagc ccctacatca aggaggccgt cgtcttcggc gagggcaaac
     9961 ccttcctcac ggcgctcgtc aacatcgact tcggcaacgt cggtgcatgg gcggaggagc
    10021 ggatgatccc ctacacgacc tacaacgatc tatctcagca ggccgatgtg gccgctctcc
    10081 tcagggaaga gctccgccgg acgaatgaac ggctccccca agcgatgcgg atcagaaagt
    10141 tcatcctcct atataaactg ctcgatgcgg acgacgagga actgacgcgg accggcaagg
    10201 tccgccgccg cttcgtctac gggctctacc tgcctctgat cgaggcgatg tacggcgatc
    10261 tgacaacgct gcccgtcaag ggcaaggtga aataccggga tggacaggtc ggggtcatag
    10321 aaacctgcgt gcgcatcctc gaggcgtgag cggagggaaa cgtctatgga ttttttcatt
    10381 caacttttgg tcaacggtct gagcgtcggc ttcctgtacg ggctgtcggc gatgggattc
    10441 gtcatgatct tcaagtcctc cagcgtgctc aattttgcac acggtgagct cctcgccctg
    10501 ggggccttcg ttttcctctc ccttcacgcg gtcgtccagc tcccgatcgc atgggctttt
    10561 ctcgcgacgc tgacggggtg cttcgtcctg ggttttctga tcgaacgttt tttcctgcgg
    10621 ccgctcatcg gcgaacctct tatttacgtg atcatgctca cggtcggcct ggcagcaatg
    10681 ttgcggggac tgctcctcct tatctggggg gggaacctgc gcaccttgcc ggattttctc
    10741 cccgcctgga tggacatcca ggcgggatgg ctccgcattc ctccggtcta tatggcggca
    10801 atcctggtcg gcatcctgtt tctcgttctg ttcgggctgt tcttcaagta ttcggcgcag
    10861 ggaatcttca tgcgctccgt tgccgacaac cagcgggccg ccctgtcctt gggcgtgcat
    10921 gtccgacgcg tctttgccct gtcctggggc atcgctgcgc tggtcgcggg catgagcggc
    10981 atcatcctcg gtctcatgaa cgggatcagc gtccacgacc tgagcgccgt cgggctgaag
    11041 gtctttcccg tggtcatcct cggtggactc aactccatca ccggcgccgt gatcggcgga
    11101 ctgatcatcg gggtcctcga ggccttcacg gggggctacc tctcgccctc cctccgggac
    11161 gtcgtccctt atatcgctct cgtcgtcctg ctgcttgtac ggccttacgg actcttcggc
    11221 ctggtcgaaa ttgaaagggt ttagggagga ttcatgagaa agcaccacct tcacccctgc
    11281 ggcaattaca aggaatcgta cacggacgaa ctggcgatct tcagctctga ttacgggcga
    11341 ctctggtcta tggccgggct cgcggcgctc ttctgcgccg ttccattcat cagtccgccc
    11401 tacctgcttt acgtcatcaa catgatcggc atctacgcca ttgcggcgat tggcctcaac
    11461 attctggtgg gatacacggg gatgatctcg ctcggtcacg gggcgttctt cggtgtgggc
    11521 gcgtacgccg ccgccgtgat gtccgcaacg tttaacgttc cctttcttct gagcgttccc
    11581 gcggccggcc tcctcaccgc cggcgtcggg atgattttcg ggattccttc cggtcgtctc
    11641 aaaggcctct acctgaccat cgcaacgctg gccggtcagt tcatcatcga atatgtcctc
    11701 gtgcactggg agggggtcac gcgcgggacg gagggaatca ccgtccccgc cccaaccctg
    11761 ttcggaatct ccctgcgggg tgacatctca tttttttacg tcattttctc cgtcctgacg
    11821 ctgatgatct gggtggccgt caatctcatc cggacgcgct acggccgtgc attcatcgcc
    11881 atccgcgaca acgaccgcgc cgccgaaggc atgggcatcc ccattttcag ctacaagatc
    11941 ctctccttcg gcatcagctc cttctacgcc ggactcgccg gcgccctgtg ggcctattac
    12001 atggtcagcg tcacctcgga gcccttcaat ctgtcgctgt ccgtcgaata cattgcgatg
    12061 gtgatcatcg gcgggctggg gagtatcgcc ggctccatct acggggcggt gttcatcacg
    12121 gtgcttaacg aatccctccg ctggctcacc gacctgatga tgaacttcgg gttcatcacc
    12181 gagtccggcc tcaatatggc cccgctccgg gaatttgcct tcggcctcgc gatcgtcctg
    12241 ttcatcctct tcgaaccccg cggcctggcg gagctctggc ggatcatccg gtccagtttc
    12301 cgcctctggc ccttttccta ttgaggggaa gtccatggaa gacacacttc tcgaaatcaa
    12361 caacatcagc gtcgtgtacg ccgatgtgat tcaggtcctc aagggggtat cgctccaggt
    12421 cccgaaacgc cggatcgtat cactcctggg cagcaacggg gcgggaaaga cgaccacgct
    12481 caaggccgtt tctgggctgc tcaaacccga aaacggccgc gtgaccgacg gcgccatcct
    12541 gtacgaggga catcccctcg aaaatcggtc ccccgagcag atcacgcgcc tggggattat
    12601 ccaggtgctt gaagggcgtc agcccttcaa atacctgacc atcgaagaga atcttcgtgt
    12661 cggcacggcc acacgctggg ggaagcccta tcgaaaggac ctggagctgg tttatcacta
    12721 tttccccgtc ctgtgggagc gccgcaaggc gctggccggt tactgcagcg gcggggaact
    12781 ccagatgctc gtcatggggc gggccttgat ggcacatccg aagctcctgc ttctcgatga
    12841 gccttccctt gggctggcgc ccctcgtggt cgaggagatc tttcgaatca tcgcccggat
    12901 caacggggag caacagacca ccatcatcct ggtggaacag aatgcccgga tcgccctcga
    12961 gatcgcccac tacggatatg tgatggagaa cggcaaaatc gtcatggagg gcacttcgga
    13021 gacgctgaaa gacaatcccg acgtcaagga attctatctg ggggggggcg cggggggacc
    13081 cggcgcctat cagcagccaa aatcctatcg ccggcggaag cgctggttat gacaggggtg
    13141 ccgggggcac tccaacgctg aggaggcaag gatggcagag gggttcgatg catgggagca
    13201 tgaaaacgcg gaggtgaggc ggacgcggaa attttctcgg atgtgcgcca tgctggaggc
    13261 tggctatggg gcgtcgtccc gtttgcgccg gatccttgat gaaacaggcc tgatgccggc
    13321 ggacttttcg cgcgacctgg atctggagcg cctgcccgtc atttcccgcg aaacgctgat
    13381 ccagtgggag ctgtccgaac ccccgtacgg cgggctggcc gaccccgccg ctgacgtgga
    13441 tcggatcttc atctcacccg ggccggtcta tgaaccccat ctgtccgaag cggacatgct
    13501 ctgggcgcgg gcctaccatg ccgcggggat cggcaaggcg gaccgcgtcc tgaacgcctt
    13561 ctcctaccac ctcgtcgccg ccggcctgac gtttcacggc ggcttgcgcc gagtgggcgc
    13621 gacggtcgtc ccctccggga ccgccggtgc ccagaatcag gtggagctga tccgggatct
    13681 cgggatcacg gcctatacgg ggacgcccag ctttctcatg gcgattatcc gaaaggcgga
    13741 ggagatgggg tatgactggg aaaaggattt tcgtctgaag cgggcctgct tcgcggccga
    13801 gccccttcgg cccgccctcc gggagacgtt cacgcgggat tacgggatcg acacctatca
    13861 gatgtacggc gccaccgagg tcggagatgt ggcctacgaa tgccgccaga agtcgggctg
    13921 gcacatctgc gaggaagtgt tcgtcgagat ctgtgatccc gaaagcggcc gacgccttcc
    13981 tgccggggaa ctcgggcagg tcgtggtgac ccgcttcaac gacatcttct tcctgatgcg
    14041 cttcggaacg ggggaccttt cccgtttgat cgaaacgccg tgcgactgcg gacgaacggc
    14101 ttaccggctg gacgggatcg cgggccgggt cggtgaggcc gtcaaggtgc gggggatgtt
    14161 cgtcgccccg agccagatcc atcgcctcgc tcagcggttt tcggggaaga tcttcagggt
    14221 ccatgtccga cgggaaggag acagggatct tctggtcgtc aggattgaag gcgcccccgg
    14281 aacgcagggc gacggctggg cgggggatga ggaattcaag cgaggattcc gcgacctctg
    14341 tacggtcggt gtggaccgcc tggaatgggt tccgaaagga acactggcgg cagcggatcg
    14401 gcagctcgtc gacgagcggg actggcgata ggccaaccct gtgcggctgc gcccctcggg
    14461 tcgcgaaggg gttgcctcag tcgacaggct gtgttccggt cgtccgcgcc gggaggtgtt
    14521 cggcgatctc gtcggccagc cgataccacc gctgcacact gttttccagg tgatagtagt
    14581 ggatgaggta ggccccttcc agaacaacga acagggccgt gaccgtccag agaagcgcgg
    14641 tcggggcgaa gaggctggcg aggaaaaaaa gcagcgcata caggccgacc gccagcgtca
    14701 catgcaggtg agcgagacgt tcgtgctgca gataggtcag gcgcgactgg tggagcgccc
    14761 gaagttccgg ccacgcatca tccgtggcgg cacccgccag ggccgtgcgg acccgctgtt
    14821 cgtggtccct tatgtagcgg atcatcccag actcctttcc gacccagcca ataggtttcg
    14881 acgacggaaa acacccagag aaacagcagg acaccaaggg aggcgacata tgcgtcggca
    14941 tgcggtcgaa gggcctgtcc cgccgcgttc agcaccgctt ccgggccgag cgcagcgccc
    15001 ggcggtgaga acgcttcgag cgactgccag atctggaaga gtgcagcgga gagcgttgaa
    15061 ccggcgacga taagaaaaca ggcgagcaga aagattcccc ggccatgacg acgaagcagg
    15121 atttcaccgg ttcccggcgc ggccagcgcg gatagaagga ccgatttggt gagggttttc
    15181 atggatacgc gggatccgca tcggggatcg ccatgtcctt cattttcaac tggatgacgg
    15241 acgacccgtt ccagtggttg tactgcgggg taaaggcgat gtccagggtc gaaccggcaa
    15301 cggcggggag cagatgaccc ttgttaaacc agatggagct gcaggaagcg ccgttcgatg
    15361 aaattctcat ccgaaggtgg ttgttcccga cgatggtcgg cgtctgaaca ttgaccccgc
    15421 ggatgcagac caccggttcg gggttcatgc tgccgaacgg cgacatcagg ccgagctggg
    15481 aaagaagttc atgattgatt tccgggagtt cgcactgggc gtcgatggtt gtctgggtca
    15541 tgaaggaggc gccggacagg tcttccgcga cgatcgcatc gaattggcgc gcgaacgaac
    15601 cgacgtctgc cgaacggatg gaaatgcccg cggcgaagcg gtggccgcca taggacagaa
    15661 gggcggaggc gcaacgtttc aatccctcat agatattgaa atcggggaca ctccggcccg
    15721 atcccttgcc gacgccgtcc ttgaggctga tcaggatggc cggtcggccg taacgatcga
    15781 ccaagcgagc cgcgacgatt ccgatcacac ccggatgcca gcgttcggag gcgaagacga
    15841 atgacgacct ccggccggag tcgggatcct tttcgatgcc ggcgatcaat tcctggagga
    15901 tccccttctc gagggcctgg cgcttgcggt tgaacccgtc gagaagcgcg gcgcactgcc
    15961 gggcctcttc ttcgtcctcc gtcaagagca ggcggatggc ttcctccggc gaggcgatgc
    16021 gtccggcggc gtttatgcgg gggatgagtc cgtaggacgc cttggacgaa tcgatcgcct
    16081 gatgatccag ggagcagatc tccttaagcg cgaccaaccc gacgcgcctg ccctccgtaa
    16141 ccagatcgag gccgatgcgg gcgaaaatgc gattctcgtc gatcaggggg gcgatatccc
    16201 cgatcgtccc gagggcaacc aggtccagat aatcccggag gttcggatag gggcggttcg
    16261 tccagaagcc ctcctggcgc agttggcccc ggagggcgat gagaaaattg aacacgatcc
    16321 cgaccgccgc cagggatttg aagggaaacg tgcagtccgc ccggttcgga ttgatcgacg
    16381 cgacggcggg gggaagggaa ggcgggactt cgtgatggtc gaggatgatc gtgtcgattc
    16441 cgagagaacg cgcatgggcg atcgcttcat gatcggaaat gccacaatcg accgtgacga
    16501 tgagacggat gccgcgggcc gccatatcgt caaccgcgcg gaggtgcagc ccgtatcctt
    16561 ctttgatgcg atcggggatg gcgtaaagga catcagcgtc gatccccttg agaaatttgt
    16621 aaagaacgac cacggacgtg atcccgtcgg catcgtagtc accgtagatc atgacccttt
    16681 cgcggttgtg cagggcgcgg gccaggcgcc gtactccctc gggcatgtct ttcatgagaa
    16741 aggggctgtg caggtcgcgc agggaagggc ggaggtaacg gcgggccgta tccggatcct
    16801 ggatatcgcg attgatcagg atctgggaca ggacgtcgtg gatgcccagc tcccgaatga
    16861 gagattcctg aagtggccga ttaccttcgg ggatcttcca gcgtgtcgtc ggcagtgtgt
    16921 ggaccatggg gactcctgat ttgtttcctt caaccgtttg gtttggcgat atatgattca
    16981 tgtcctttcc ggtgtcaagg aaagaatgag aacggttggc gaaacaggca gaattcctct
    17041 tgcatcttga tatttggcgg gcgaagtgat agacagaagg ggaagtcgtg gtgggatatt
    17101 cggaccgcgg caacggggcg ttgatcaaat gggagggaag atgtcgagaa gaaggctcct
    17161 ttggatcgtg atgctcgttc ttcccctggt cctggggatg ggttcccgat ccaacgattc
    17221 cccggagaaa atcccggcac ctatgaagaa attccgggca tccttcattg atcagatgga
    17281 tgtgctcacg cagtgtacaa acgtcagcat cgatggggag acctacctac aggggaagcg
    17341 gggcgagggg aattacacga tcgggttcga ccggatcgcg tccgtggttc tccacttgtc
    17401 cggaggcaaa ctcatcgggg tggtcaagct gcgcgacggc acgtccctgg agctcattct
    17461 gaacggaagt cagaaagcct acggcttcac gggctacgga acgtttcaaa tcaggctcca
    17521 ggatttgaag aaaatgacga tcggcgggaa agggtagagg gcccggcgga cggatgccgc
    17581 cgggcaaaga ccttatacgg cctttttcac gaatccggaa cgcagacagc gggtgcaaac
    17641 gcggacgcgc ttgatggcgc ccgtcttctg gtcgatacac cgcatgcgct gcaaattggg
    17701 ataccagacc ttcttggttt tgttattggc gtggctcaca ttgtggccga acgtcggtcc
    17761 ttttccacaa acttcgcaca ttcttgacat ttttccactc cccctgacca aactgtgcgc
    17821 gatacctaaa gcattcacac ggatattgca agcatttttt tgcacacgtt ttcctgagat
    17881 cgccgggcgc cgcggttaat tcttcaggac cggaggctgg cccgtcgatt cgaggacgcg
    17941 catccagatc attccttgcg gatcgagctt ggctttggag gaaaccatct tcaggggcaa
    18001 atggacgtat tcgtcctgca tcaacccgac cagcatcccc gttttcccgg ccatgccggc
    18061 atggaccgcg tactgtccca atgcactgca gtagatgctg tcgctggcgt tggcggtgac
    18121 gctccggatc atgtagctgg ggtcgatata cttcaggttg acctcgatgt tcagccgcga
    18181 gaaatgctcc tcgatccggg tcttcagaaa gaatccgatg tctttcaggc ggggattgcc
    18241 ggaggcgtcg gtttcaaccg tgccctgacc cggctcgatg tgctcctgtc ccgcaccttc
    18301 cgcgacgaga atgaccgcgt gatgccgatg ccgcaggcgt ttttcaagcg cggccaggaa
    18361 cccattttcg ccctcgaggt cgaactgaac ctcggggacg agaacgaaat tgacgtcatt
    18421 ttgggcaagc gcggaaccgg ccgcgatctg accggattga cgccccatga tcttcaccaa
    18481 gccgatgccc atcggagccc ctttggcctc cacgtgggcg cagcggatcg cgttgacggc
    18541 ctcggaaatg gccgtgtcgt agccgaaggt cttctgaatg agcgtcagat cgttgtcgat
    18601 ggttttgggg atcccgatca cgcttatgtt cagcttgcgt cgcgtgatct cgtcggtgag
    18661 cgcctccgcc gcacgcatgg tgccgtcccc gccgatgcag aagaggatat cgatgttcat
    18721 ccgcttgagg gcgttgacca tctccacgat atcctgccga ccccgggagg acgaaaggag
    18781 actccccccg acgccgtgga tgttctccac ggtgtcggga tgcagttcca tgagttcata
    18841 accgaaacgg ggaatcaggc cgcggaatcc gtagcggatg ccgatgatgt tcctaacttc
    18901 gtagcgatgg tacagctcca tgacgatggc gcggatcacg tcgttgattc ccgggcacaa
    18961 tccaccgcag gtgacgatgg ccgccttggt ctgaatcggt tcgaagaaga tcttgtcgcg
    19021 tggacccgcg agttcgacgg acaggggggc ggcgtccggt tctttccgga gatcgtcttt
    19081 gtcgggcagg aagtgatcga acaggacccg tttgctgtcg gacgtgaaaa agctgacgtt
    19141 caagggggag tccgtggtgc attctcccag cgactgaatc gtgaaatcat aggggagatg
    19201 attcctcatt cctcattcct ccttcccgaa gtcaccgggt cccgattcga gggccggttg
    19261 atgtagggcc acccggccgc aggtgcggcc cgcgccgagg aagaccttgc gaccttcgac
    19321 gcgtaaacgc ccctccgcat accactggat ggcctgggga aagatacggt gctcttgctc
    19381 gagaatccgc gcgccgaggg tggcttccgt atcggcctcc tcgaccggaa tcacggactg
    19441 gacgatgatg gggccggtat cgacgccttg atcgacgaaa tgaaccgtgc agccggagaa
    19501 tttcacgccg taattcgcgg cctgcctctg gctgtgcacc ccgggaaacg ccggaagaag
    19561 cgcgggatga atattgatga cccgcatcgg gaaggtcctg aggaatgttt ccgaaaggag
    19621 gcgcatgaaa ccggccagga cgaccagctc caccccgtat tcgtttagga cttggatcat
    19681 ggccgcgtct gccgcttcgc gcgacggatg tgacccggac ggaaccttga cgacagggat
    19741 gccgtggcgc gctgcccgct caagggcgaa cgcccgggga ttgtcgctga tcacgacggc
    19801 aatgcgtgca tcaagggccc gtgcctcgat gcggtcgatg atggcctgga gattcgatcc
    19861 gtttccggaa gccaagacac ccagtgtgac gatccgtttt ttcatgcgag ggactccagg
    19921 ggggtaggca tccggggatc aggccggcgg gagcaggacg acggagggct gcgcggaatc
    19981 ccgcttatcg atttgcccga taagataagc tttctctccc atctgttcca gccgctcgag
    20041 gatctcaccg ctttccttct ccggaaccac cagcatcatg ccgatgccca tgttgaacac
    20101 gcggtacatc tcgtcgtcca cgatgttccc gatttcctgg atcgccgaaa agaccggatg
    20161 gatcggccaa gcgcccatct gaatgatgac gcgggaggcg gcggggatga tccgtgggat
    20221 gttgtcgata aaaccgcccc cggtgatatg gacgatgccc ttgaccgtga agttcttgat
    20281 gaggttcagc accgtccgca cgtagatccg tgtcggtgtc aggagctcgt cgccgatggt
    20341 gttgtcgaat tcctcaaggc gatcgcccag gcgatagccc ccccgctcga acaggacatg
    20401 ccgggccagg gaaaagccgt tgctgtgcag gccgctcgag gccagaccga taatccggtc
    20461 cccgacgcgg atgcctgatc cgtccagcag tcggtcggcg tcgacgatcc cgacgcaaaa
    20521 gccggccaga tcgtattcat cgtcgcgata gaatcccggc atttcggccg tttcgccccc
    20581 gatcagggca cagccggcgt cctggcagcc tcgaacgatc ccgtccagga tttccagaca
    20641 gcgcccgacg tcgattttcc ccagggagag ataatccagg aagaagaggg gttcggcgcc
    20701 ctgaacgacg atgtcgttga cggacatggc gaccaggtcg atgccgatgg tgtcgtgccg
    20761 catcatctct tgggcgattt tgagttttgt tccaacgccg tccgtggacg aaacgagcac
    20821 gggatcgcga tacttttccg gatcgaggtg gaacaggccg ccgaaaccgc cgattccact
    20881 catcacttcc ttgcggaagg tggtcttgac gagaggttta aggcgctcga cgaagcggtt
    20941 cgcctcgtcg atatcgaccc ctgccgtctt gtacgaggca ttgtctgaca tgtggtctcc
    21001 gagaaaaagg atgaaggttg tgccattacg gcgcaaatcg atggatctgc ttaacctacg
    21061 gagggacaaa agtcaaatca ttccttgcgg caagtggaga tttctgataa ccatcaagtt
    21121 gttcttaatg ctgaaatcat tgaactaaat caggcattgc gcatgtccga ccttccaaat
    21181 cgtctccagg aaagcattcc gctgccttct ccacagcccg aactcgaggg aggccctccc
    21241 ggcgcggtgc tcacgcgccg gttcgcgcgt ctcgatcagc cggttcgttt cctcaagggg
    21301 gttggtccga agatggcgga acgactggcc cgtaaggatc tgaacaccat agaggacctc
    21361 ctctactttc tcccgcgccg ttatgaagat cgccgtaacc tgaagccgct cgcacagatt
    21421 gaagcgggaa gacgggaaac cgtggtcgga acggtcgtcc ggagcggcct gacgtcccgc
    21481 ggatatcgcc atcaattcga ggttaccttc aaggacgacg gcggaacgat gaccgcgcgc
    21541 tggattcgcg gacggtttgc ctatctgcac gggatcttcc gggttggtgc gatgttcatt
    21601 cttacggggg atgtccgctc tacttattcg ggtccggaga tgatccatcc cgacttcgaa
    21661 ttcctcgacg gtgaggatga aagagaccga ccccccttcc ggaggatcgt tcccgtctac
    21721 tctgagacgg aaggcctgcc gcagaaaacg atccgccgca tcatgggggg gctcctggag
    21781 acgcatgcgg aggatctggt gagccccata ccggcgtccg tctgccggcg tcagcgcctc
    21841 atggacatgc cggaggccgt ccgacgtgtc cattgccccg atcttggcga ggagatggac
    21901 gacctgaacg ggatgcgctc cgcgtcgcgg cggcggatcg ttttcgatga gcttttcttc
    21961 ttccaactcg gcatggccct ccgctatcgc ggaaacgtca tggagccggg ttgtcccctc
    22021 aaaacgcgcg gaccgatgct aaagcgtttt ttcgaggcgc ttccgttccg gatgaccccc
    22081 gcccaagaac gcgtctggtc cgaaatcgcc catgatcttg accgtccgtc cccgatgaac
    22141 cgtcttctgc agggcgatgt cggcagcggc aagacggtgg tcgccctggc ggccctgatc
    22201 acggcctgtc agaacgggtt tcaggcggcg ctcatggcgc cgacggaggt gcttgccgcg
    22261 cagcaccacc ggaccattcg gcactggctg cagcccttgg gtatcgaggc tgagctcctg
    22321 accggttccc tgacggtacg ggaaaggcag tcggtatacg gcgggatgca gcggggcgcg
    22381 attcaggtcg ctgtcgggac ccaggcctta atccaggcct ccgtatcctt tcaacggctg
    22441 ggcctcatcg tgatcgacga gcagcatcgc ttcggcgtcc ttcagcgagg ttccctccgg
    22501 ggtaaaggcc gccatccgca tgtcctggtc atgaccgcca cgccgatccc ccgcaccctg
    22561 gccatgacgc tttatgggga cctcgacgtg tcgatcatcg acgggctgcc gcccgggaag
    22621 aaatcgatcc ttacccgcgt cttgacggag ggtcagagat cctcggccta ccgggccgtt
    22681 cgtcaggaag tcgaacaggg ccgccaggtg ttcatcgtct atcccctggt cgaggaatcg
    22741 gaggtcctgg atttgaagga tgccacacgt atggccgaac atttgaaggc agatgtcttt
    22801 ccgaattgcg tggtcggtct catccacggc cgtatggccg ggacggagaa ggacatcgtc
    22861 atggaacgat ttgccaaggg ggagattcaa atcctggtgt cgacgtctgt tatcgaagtc
    22921 ggcatcgacg tgccgcgggc ctctttgatg gtgatcgaac acgcggagcg cttcggtctt
    22981 tcccaattgc accagctgcg cgggcgtgtc ggccgcggtg agatcgcctc cgcgtgcctg
    23041 ctcatggtcg gaaagggggt aacgttgacg gcgcggcgac gcctgcggat catggcggaa
    23101 tcgaacgacg ggtttcgcat tgcggaagaa gacctggcca tccggggacc gggtgagttc
    23161 atgggcacgc gccaatctgg aatccctgat tttcgcgtgg cccacctcct cagggacatg
    23221 agcgtccttc aagcggccaa ggaggaggcc ttccgggtcg tgagcgaaga tcccacgctg
    23281 acgcatccac ggaacgaggc gttacgtcag gtgctcttcc gccgctggga tggccggctt
    23341 cacctcgctc ggaccgcgtg aacacccgga attcgctttg acaaatcggc gaaaactgat
    23401 atagaagctg cgccgctgga ggaacgaata tcatgcgaac catcgggctg ggattaatgg
    23461 gtttcggaac cgtcggaacg ggcgtcgtca aactcctgca ggagaaccgc gatgtcctgg
    23521 aggcgcgtct cggcgcatgt cttacactca agaaaatcgc ggatctggac atcgtcagcc
    23581 ccagaccggt ggcggtcgat cccggtcttc taacccgccg ggcggaggat ctgatcgagg
    23641 atccggatat cgatatcgtc atcgagctca tgggagggtt cgatcccgcc cgcacactga
    23701 ttctgaaggc atttgccagc ggcaaacatg tcgtcacggc gaacaaggcc ctgttggcga
    23761 cccacgggaa cgacattttc tgcgaggccg aggcccgcgg agtgaacatc ggcttcgagg
    23821 ccagcgtcgg cggaacgatc cccgtcatca aaacgctgcg cgaagcgcta ggcgccaacc
    23881 acatccggtc gatcttcgcg atcatgaacg ggacctcaaa cttcatcctg agcaggatga
    23941 ccgacgaggg tgaatccttc gacacggttc tgaagcgggc gcaggtcctg ggccatgccg
    24001 aagcggaccc gacctatgac gtggaaggaa tcgatacggc gcataagctg gcgattgtcc
    24061 tttcgctggc atacggccgt cgcgtccgtc tggaggacat ctaccgagag ggcatctccc
    24121 atctgacgcc gcaggacatc gagctggcca gagaactcgg ttataggatc aagcttttgg
    24181 ccatcgccct cgagagggag gacggcatcg aggcgcgcat ccaccccacg atgattccct
    24241 tcaaacacct cctcgcgagc gtgaacagca acttcaacgc cttccacatc atcggcgacg
    24301 ccgcggattc ggtctttctt tacgggcagg gctcgggcat gatgccgacc gcgagcgccg
    24361 tggtcagcga cttgatggat atcgcgcggg acattctgaa gggcatctcc tgccgtgtcc
    24421 cgagccgtgc gcttcacgag gcgtcgatgc ccgatatcgt gatccttccg atggccga
//
LOCUS       DAOOLJ010000024        23868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2003_length_23868_cov_15.988284, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000024 DAOOLJ010000000
VERSION     DAOOLJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 23868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23868
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2003_length_23868_cov_15.988284"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLW16_05775"
     CDS             <1..1205
                     /locus_tag="PLW16_05775"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB70450.1"
                     /translation="YVLCDLIEIPNLGTRANRYLGYLAQRYLIRSEEAEYPLSDKGEA
                     LWLFFYGVSSFCYRIYITLKIALFVAGKFFFIGVLIALWALIGLLVVPLVRVIRTVMS
                     QHDLYQLRARILTLGIVAAVGLLLFMAVLSFPSFTTAEGILWPGDEAQIRTGAEGLVR
                     EIAAVPGTHVKTGQILIRCENSELVFQVKILEAERREYEARYRNAFARDRNEERIMRE
                     EIARIDEAIYKAREELSALDVRSPAEGILILPQAEDLPGRFIKRGEALGYVVDYNKIN
                     VRVVIPQTDVDRVRNNVRSVQLRLAETPSVELTSEIIREVPAASSHLPSLALSLEGGG
                     AIALDPTADEGSKSFENYFHFDLRMPERSQVRIGERVYVRFEHAPESLARRGYRTMRH
                     LFLRTFDL"
     gene            1287..3293
                     /locus_tag="PLW16_05780"
     CDS             1287..3293
                     /locus_tag="PLW16_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPB70451.1"
                     /translation="MMRDVGTWPPADYFVRPERRGHRRTLLDRMARELAGYVIRPARV
                     RLLNPDRIVAGIRRLEEALRVKTDGELKDLADSMRRRLRSEGFKDPVVTEAFALIREV
                     AGRTVGMRHFDCQLIGGAALLKGFLVEMDTGEGKTLVATLPAATAAMAGLPVHVITVN
                     DYLTARDAEWMGEIYRFLGHSVGCITHEKTPVQRRQAYRADITYCTNKELVFDYLRDR
                     LLLGEASDPLRLYGEHLYDTKGKSDRLLLRGLHYGIVDEADSVLVDEARTPLILSKKE
                     NAGREEEVAQQALTAAAKLVEGDDYRLEYETDQGLRKVVITEGGRETIRRITGPFGIQ
                     WRGAVRREELVKKALAATRLFKRDEHYLVRDGKVQIVDEFTGRVMPDRSWEGGLHQLI
                     EVKEGCEITGLQETAARISYQRFFMKYLGLCGMTGTAEEVRRELWSVYALPFIRVPTN
                     KPVRRRSGPDRLFASDDEKWQAVVRRVREIHDKGRPVLVGTRTVAASEHLGALLVEEG
                     LSFQILNAKQDGEEAGIVERAGEAGRITIATNMAGRGTDIKLAPGVAEKGGLHVLITE
                     RHEAGRIDRQLAGRCGRQGDPGSYEAFLSLRDPLMENAFTGGWGQVERLFRRSGYDIR
                     RIAGRRMMIRAQKKVEKAHAGVRKRLLKYDEQLGDALSFSGRTD"
     gene            3299..4030
                     /locus_tag="PLW16_05785"
     CDS             3299..4030
                     /locus_tag="PLW16_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB70452.1"
                     /translation="MKPTHSIIPVLLICLSGLFCLGAAPTESLKGLLEPSEIVELGSQ
                     TPGIIEEFLADRGDRVKKGQVLVRLQAAVEKATVDLAVARLEFGKRKVQRNEELYQKQ
                     LISIHEKDEMETEVKIAELQLREAMEKLRLRTITSPIDGVVVKRLRSPGEYVGEEPIM
                     TLASVDPINVEVFVPASWHRRIRKGMQVEVRPEAPLSGVYRAKVAVVDEVIDAASGTF
                     GVRAELPNPDHRVNAGLNCTVRFLP"
     gene            4118..4459
                     /locus_tag="PLW16_05790"
     CDS             4118..4459
                     /locus_tag="PLW16_05790"
                     /inference="COORDINATES: protein motif:HMM:NF022841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3192 domain-containing protein"
                     /protein_id="HPB70453.1"
                     /translation="MKKVIVLVAMCVLFCSCASSAILKTNENLLRLKTGMAQDEAMTI
                     MGTPTLSELYEAADGNQVSILYYRTERKETTVLSAKDECTPVVFTNGKLVGWGDRLTA
                     ADINLLKVKTK"
     gene            complement(4469..5791)
                     /gene="trmFO"
                     /locus_tag="PLW16_05795"
     CDS             complement(4469..5791)
                     /gene="trmFO"
                     /locus_tag="PLW16_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPB70454.1"
                     /translation="MPSAGTPITIIGGGLAGCEAAWQLLAAGHRVTLVEMKPERFSPA
                     HTSPLLAELVCSNSLRSDRIQNAVGLLKAEMRALNSLILAAADASAVPAGRALAVDRE
                     AFSRQVEDGLQRKPGFTLVRREVQEIPSEGFVLIATGPLTSDALAANLAERTGAKHLA
                     FYDAIAPIVTGESIDRTRVFRASRYDPEGGGDYLNCPLNLEEYERFLNALLTGEKVPL
                     HTFEETKCFEGCLPIEVLAERGPRTLTFGPLKPVGLTDPRTGRQPYAVVQLRHENLAA
                     TLFNMVGFQTKLTWPEQRRIFRMITGLEQAEFTRYGSIHRNTFLNAPAVLRETLQLKT
                     DDRIFFAGQITGVEGYVESAAMGLIAGLSLSRHADGKTFLPPPPTTALGSLIRFLTAS
                     DPTAFQPMNVNFGLLTPLGGRVRKPDRGDRYAARALTDLAAWVRSLRL"
     gene            complement(5818..8145)
                     /gene="topA"
                     /locus_tag="PLW16_05800"
     CDS             complement(5818..8145)
                     /gene="topA"
                     /locus_tag="PLW16_05800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB70455.1"
                     /translation="MSESLIIVESPTKVKTLRKYLGPEYDVRASVGHVKDLPKNRLGI
                     DPDKDFEPTYLVMDAKKKVIAELKKAAAKAHQILLAPDPDREGEAIAWHIAEEIGGRG
                     KTVRRVLFNDLTRATILEALRNPRELDFDRYEAQQTRRILDRLVGYQISPILWDKVKR
                     GLSAGRVQSVAVRIICDREAEIKAFVAEEYWTLTALVQGTNPPPFEAKLIKIDGKKAK
                     VVNAEGSDEIVRSLREAPFTVSSVEKKEVRRQPPPPFTTSKLQQEASRWFRFSAKKTM
                     STAQKLYEGIELGAEGPVGLITYMRTDSVRIAADALTDAREFIRRSYDPTFLPPKART
                     FKVSGKAQDAHEAIRPASMSHPPQTVKPFLSNDQFRLYQLIWNRFLASQMNPAVLDQT
                     TVDITAAACLFRAQGAVMKFPGFTIVYTEGKEDNGDENGIGKLLPEVRKGEVLALLDL
                     KPEQKFTQPPPRFSEASLVRELEENGIGRPSTYATILSTIQEREYVRLEKGRFIPTEL
                     GILVTDLLVKNFPRILDVTFTAGMETELDRVEDGQARRLDILNNFYRPFTEELMKAKA
                     NMTNVKREEIPTDLVCEKCGKPMVIKWGKNGRFLACTNYPDCRNTKNFTQDDNGDIQP
                     ARDAVTDIACPLCGKPMLAREGRFGKFLGCSGYPECRHTMNVGPDGNPITSPTEAPEG
                     ADLCEKCGKPMVVKRGRYGNFLGCSGYPECKHIVRIPRGGKAGAPAAEAVITDTLCDT
                     CGKPMALKTGRFGKFLGCTGYPECRTIHKYKPSNP"
     gene            complement(8130..9290)
                     /gene="dprA"
                     /locus_tag="PLW16_05805"
     CDS             complement(8130..9290)
                     /gene="dprA"
                     /locus_tag="PLW16_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPB70456.1"
                     /translation="MQKESLPDWLALKKVEGVGNLAFKTLVEALGSPAKVFAASYETL
                     KRIPGIGDKTAAQIKSFDDWKACEKELERVRQGSMTLLTFQDADYPRRLLNIYDFPPL
                     LYVRGTLSNEDIPIAVVGSRQASPYGRFTTERLCRELALRGITVVSGMARGIDSAAHS
                     GALAGRGRTIAVLGSGPDVIYPPENESLYRKIAASGAVISEYPFGTPPNAANFPARNR
                     IISGLSLGVVVVEAGMKSGSLITARIALEQGREVFAVPGAIDSSGSRGTHRLLKEGAK
                     LIEGVDDILEEILPQLQRAGIVPPAAQPSPDAIPDAATSDGAAMESLSERERIVFSAL
                     SAIPVDANALVHATGLTVQEIITALLQLELNGLVRQMPGNRYLREESLCRNR"
     gene            9447..11120
                     /locus_tag="PLW16_05810"
     CDS             9447..11120
                     /locus_tag="PLW16_05810"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70457.1"
                     /translation="MKRSSGLVARILVATILAVPLTYRPLILSAQTTPAPAPVINVAP
                     HEIDLGGLTSGQTLKGKFILSYPGPASQAWNTQTLSGWTFLDQDRLSGTVAEEPCVLE
                     VRLSRFDDGEERDVDAQDAAIRSVRLSLESGGKAITARREFSAGPHRVAIPVSLPGGV
                     RNLHIRFSLPYPELETFLTVDPPRLDFGSLGPGETSTRIIRLNNVGRERLHWKAVGPA
                     PEKEVVPVSRGRYHSFQNPELRGQGTYQPPVRIAEAVEFPPGWSEEGGYPVSHPQGSN
                     MKCRFKGTGVVLHLWKSSDGGSLSIYIDNRLVETVNAISDPVERFTSNGVSGLNEGPH
                     ELSVVVKSGRLKLEGMTVYDQDVRRGPADWINLFPDSGVITRETDFVNIALRAQNVPP
                     GTYRDHLLIRSTGGDQVVDLSVEVRADAPTKIIDVYRYFRDNDCLFTVNPEAEVQRLH
                     SGGYQRQGVAFRLFIPGIPGTTELYRWFNPKKRDHYYAINRLAVNPQSGYVLEGVIGH
                     IATSRMNNTRPLYRWRNPRTGHHFFTTDQSGEGRDRKGFRFEGIVGYVR"
     gene            11191..11267
                     /locus_tag="PLW16_05815"
     tRNA            11191..11267
                     /locus_tag="PLW16_05815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11225..11227,aa:Arg,seq:tct)
     gene            11278..11354
                     /locus_tag="PLW16_05820"
     tRNA            11278..11354
                     /locus_tag="PLW16_05820"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11312..11314,aa:His,seq:gtg)
     gene            11387..11463
                     /locus_tag="PLW16_05825"
     tRNA            11387..11463
                     /locus_tag="PLW16_05825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11421..11423,aa:Arg,seq:tcg)
     gene            11497..11572
                     /locus_tag="PLW16_05830"
     tRNA            11497..11572
                     /locus_tag="PLW16_05830"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11530..11532,aa:Lys,seq:ctt)
     gene            11629..12960
                     /gene="miaB"
                     /locus_tag="PLW16_05835"
     CDS             11629..12960
                     /gene="miaB"
                     /locus_tag="PLW16_05835"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPB70458.1"
                     /translation="MEKKRLYIRTFGCQMNVHDSEQLAALLAYEGYERADRPEEADLI
                     VVNTCSIREKAAQKVYSELGRLRSLKSLKPGLRIAVGGCVAQQRGRALLVAAPHVDLV
                     FGTHQLASVPRLLNLCGHGGAGVVETAFCDRVESLAIFAPPPHGTVSAYVTIMQGCNN
                     FCAYCVVPYLRGREQSRSGREIIAEIRSLADLGIREVTLLGQNVNSYGVGTPEGMDFP
                     GLIREIGAVSGIERIRFTTSHPKDLSADLMQCFREVESLCGHIHLPVQAGSNRVLVRM
                     NRGYTREGYLDKVQRLRDACPNISITSDIIVGFPGETEADFRETLDLMGKIRFDNLFS
                     FKYSRREGTAAMKMTEPVAEEVKSERLKILQALQDEHTQERNRLSIGRVEEILVEGVS
                     RASSRHLTGRTRGNKIVNFAGEERLIGRTVRCRITEAFLHSLRGELQLPEA"
     gene            12963..13463
                     /locus_tag="PLW16_05840"
     CDS             12963..13463
                     /locus_tag="PLW16_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPB70459.1"
                     /translation="MLIEMKISGLTIDPLTNTPIVILKDLEEKKAIPIWIGLFEASAI
                     ATEMEKISFARPMTHDLLNEILKTLDVQVTRVEINDLRNNTFFANIFVVREGRDYVID
                     SRPSDALALALRARAPIFVEQKVIDKSRNVDFGSKGTDQDQLQEEKLKEFLENMSPEE
                     FGKYKM"
     gene            13592..14536
                     /gene="xerC"
                     /locus_tag="PLW16_05845"
     CDS             13592..14536
                     /gene="xerC"
                     /locus_tag="PLW16_05845"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPB70460.1"
                     /translation="MIEDVEQALRRFSRHMEMERDLSPHTRSNYLHDVRQFVAYLEAE
                     ALIDAAKGQGLESIDALMIRTFLAGLYQRRIRKVSIARKISALKSFFRYLQRDGVLKG
                     NPAELIQTPRIETYVPSVLSADEMEDLLKAPQDARFSGARDRAVLELLYSSGIRLSEL
                     TGLNVGDVDFAAGLVRVRGKGKKDRIVPVGGPALNALRVYLEARDALFPEPDGVGQQP
                     IFVNRRGGRISTRSIARIVDRSTVQGGLRRKISPHTFRHTFATHMLDAGADLRAIQEC
                     LGHESLSTTQKYTAVSVARLMEVYDKAHPKAGGGHKKP"
     gene            14533..15111
                     /gene="hslV"
                     /locus_tag="PLW16_05850"
     CDS             14533..15111
                     /gene="hslV"
                     /locus_tag="PLW16_05850"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPB70461.1"
                     /translation="MIMHGTTIVAVRHKGKVTVAGDGQVTIDVTVMKHGARKVRRLYH
                     NEVIVGFAGATADAFTLFDRFDQKLEQYNGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VNRESFLMISGIGDVIESDDNVMAIGSGGPYALAAARALIRHTDLTAAEIAREAVSIA
                     SEICIYTNDNITLEELDTAEVPEGTSRSRSGS"
     gene            15120..16496
                     /gene="hslU"
                     /locus_tag="PLW16_05855"
     CDS             15120..16496
                     /gene="hslU"
                     /locus_tag="PLW16_05855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPB70462.1"
                     /translation="MSENGLTPKRIVEKLDRYIIGQGEAKRAVAIALRNRWRRQNVVK
                     ELADEISPKNIIMIGPTGVGKTEIARRLAKLDNSPFIKVEASKFTEVGYVGRDVESMV
                     RDLVELAVNMIKGEEREKVRARASELAEERLLDVLLPARPEERRAEDLIAGHPGTSPD
                     AAPAVDTTREKLRRLLREGKLDQRPVDLEVSEARTSPMIEVFSSTGIEDLGLNIKEVF
                     GNIFPQKKKQRRVKVPEAIEILTDEEAQRLVDMDKVTRAAVERVEQSGIIFLDEIDKI
                     VGGDGAHGPDVSREGVQRDLLPIVEGSTVNTRYGMVKTDHILFIAAGAFSSTKPSDLI
                     PELQGRFPIRVELDSLGKEEFIRILTEPHNALIKQYREMMATEGIDMVFTDDAVAEIA
                     EVAETVNERTENIGARRLYTIMETLLDEVSFDAPDMEEKQVVIDADYVRERLDDIVED
                     EDLSRYIL"
     gene            16508..17401
                     /gene="argB"
                     /locus_tag="PLW16_05860"
     CDS             16508..17401
                     /gene="argB"
                     /locus_tag="PLW16_05860"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPB70463.1"
                     /translation="MVDPIQQSMARADVLLEALPYIRRFYNKTIVIKYGGHAMADDAL
                     KNAFARDIVMMKYIGINPVVVHGGGPQIGSFLKKLGKDSTFIQGMRVTDEETMDIVEM
                     VLVGKVNKEIVGLINQHGGRAVGLSGKDGGLIRAEKYLLSAEKAKDTPPEIIDLGLVG
                     KVREIDGSLISVLVKDGFIPVIAPTGAGESGETYNINADIVAGAVASALKAEKLVLLT
                     DVAGVLDAEKKLVNTMNNRAAQRMIETGIIEGGMFPKVKCCLKALRGGVHKTHIVDGR
                     LRHAILLEIFTDSGIGTEIVA"
     gene            17523..18710
                     /locus_tag="PLW16_05865"
     CDS             17523..18710
                     /locus_tag="PLW16_05865"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPB70464.1"
                     /translation="MNKTEWMGLGERYLMNTYRRAPLVFERGEGVRLWDCDGREYLDF
                     LAGIAVCSLGHAHPRIVEAIRRQAGRLMHVSNLYQIEPQILAAQALCERSFAEKVFFC
                     NSGAEANEAAIKLARKFGHERLGGRYEIVTMINSFHGRTLATITATGQTKFQAGFEPL
                     PAGFRYAPYDDPKALADAVTDRTCAILVEPIQGEGGIRLPAPTYLQEVRALCDARGIL
                     LMLDEVQTGIGRTGTLFAYEQAGIKPDVMTLAKALGNGFPVGAMLSTEEVARVFEPGT
                     HASTFGGNPLAMAAVLSVLEAIDEEGLLKNCQEMGEYFLQKLSVLKRKHSLIRDIRGR
                     GLLIGCELGCEGAPYVSRCMQEGLLINCTNANVLRFAPPLVVTAGDIDRAADILDAAL
                     GAS"
     gene            18707..19615
                     /gene="argF"
                     /locus_tag="PLW16_05870"
     CDS             18707..19615
                     /gene="argF"
                     /locus_tag="PLW16_05870"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPB70465.1"
                     /translation="MKKDLLSEFDLAKDDFECIFEKAKRQKRIHEEGRFYQPLRGKTL
                     GMIFDKSSTRTRISFEVGMYQLGGIALFLNSRDTQLGRGENIMDSARIMSRYLDGIMI
                     RTFSQKAVEEFARHASIPVINGLTDLLHPCQILSDLFTIMEKKGAYEGKTIAYIGDGN
                     NIANSWINAAARLPIRLALACPEGYRPDRRILARALKNNGADILLTDRPEEAVKGADI
                     VYTDVWASMGQEHEQEERAGRFQRFQVNERLLKHARKDALVMHCLPAHRGEEITAEVL
                     DGPQSIVVDQAENRLHVQKAILEILL"
     gene            19629..20846
                     /locus_tag="PLW16_05875"
     CDS             19629..20846
                     /locus_tag="PLW16_05875"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPB70466.1"
                     /translation="MSDNIKKVVLAYSGGLDTSVIVRWLIETYGCEVVCFAADVGQKE
                     ELDGLEAKAINTGASKCYIEDLREEFVRDFVFPALRANAIYEGTYLLGTSLARPLIAK
                     RQIEIAHAEGADAVCHGATGKGNDQVRFELTYIALDPAIRIISSWKDPHWTFRSRPDM
                     IDYAEKYGIPLPLSKEKPYSADRNLLHMSHEGGILEDPWAEPPEDVFVLTVSPEDAPD
                     RPTYVEIGFERGNPVAVDGVAMTPAALLDRLNDLAGANGVGRVDMVENRFVGMKSRGV
                     YETPGGTLLWAAHRALESITMDREVMLMRDSLTPKYAQLAYNGFWYAPEMETLQRYID
                     ATQENVTGTVRVKLYKGNCIVVGRKSPHSTYREDFATFDQDQVYNQMDATGFIRLNAL
                     RLRIRSLIKGRSA"
     gene            20881..22281
                     /gene="argH"
                     /locus_tag="PLW16_05880"
     CDS             20881..22281
                     /gene="argH"
                     /locus_tag="PLW16_05880"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPB70467.1"
                     /translation="MIPINKTKKLWGGRFKEPTDRLVEAFTASVQYDRRLYRYDIEGS
                     IAHTRMLVKQGILKKKEGTLIITGLKKILKDIQEETFTFRTGDEDIHMVIERALIRAV
                     GDVGGKVHTGRSRNDQIALDIRLYLRDEIREVLVRIAALKDTLVDLAKEEFGTIMPGY
                     THLQKAQPVLLSHYLLAYWEMLDRDEQRLADSLKRVNVLPLGAAALAGTGLPLDRQYT
                     AELLGFESVSRNSMDTVGDRDFVAEFIFTASVVMMHLSRFCEDLILWSTEEFGFIEIA
                     DAYTTGSSIMPQKKNPDVAELVRGKTGRVYGHLVTLLTLLKGLPMSYNRDLQEDKEPL
                     FDSVDTVKACLDVFTAMLRHTRFNRERMLKEADRGFAVATDVAEYLTRKGIPFREAHR
                     IVGRIVGYCLENDKSFDDLTLKEWRKFDRKFTQDIQDALAVETSVDARMTAGGTAREL
                     VRKRIEEIERQREPKA"
     gene            22265..22702
                     /locus_tag="PLW16_05885"
     CDS             22265..22702
                     /locus_tag="PLW16_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70468.1"
                     /translation="MNRKPDRNGASRWILAILIVILAVTVLWACGRKADPLSPRVEAP
                     AAIGGLTVERFPEGFRLGWAPPGAGVARIRILRSDVTDASCPGCPKESRLITELIPDD
                     DGVLPVTYTDRSVKEGADYTYRLIACGADGVCGDESAETGIKD"
     gene            22712..>23868
                     /gene="lysA"
                     /locus_tag="PLW16_05890"
     CDS             22712..>23868
                     /gene="lysA"
                     /locus_tag="PLW16_05890"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB70469.1"
                     /translation="MNDFKFRQNRLYCEDTPVEEIAGAVGTPFYLYSFRTLTRHFQVF
                     DEAFADVPHLVCYAVKANSNIALLGIFARAGGGADIVSGGELYRALKAGVPPAKIVYS
                     GVGKRIDEIEYALREGILMFNVESPQELELIDETAARMGKRTGIALRVNPDVDPETHP
                     HISTGLKENKFGIDVSRSLEAYRRAKRLSSLDIMGVSCHIGSQVTKTSPFVEALERIR
                     RLIVLLREEGIAIRYLDLGGGLGITYDRETPPHPTEYARALLEASREIDCTFILEPGR
                     VIVGNAGILVTRVLYTKETEDKRFLIVDAGMNDLVRPSLYQAYHEIRPVVDEDREERV
                     YDVVGPICESGDYLAKGRSLPDQKRGELLAVMSAGAYGFAMASNYNSRPRVP"
BASE COUNT         4817 a         6849 c         7711 g         4491 t
ORIGIN      
        1 attacgtcct ttgcgatctg atcgagatcc cgaatctggg aacgcgcgcc aatcgctatc
       61 tcggatatct ggcgcagcgc tatctgatcc ggagtgagga agcggagtat ccgctctccg
      121 acaagggaga agcgctctgg ctgttcttct atggcgtcag ctccttttgt taccggatct
      181 atatcaccct gaagatcgcc ttgttcgttg ccggaaagtt cttctttatc ggggtgctga
      241 tcgccctgtg ggcgctgatc ggcctgctgg tcgtcccact ggtccgggtg atccgcacgg
      301 tgatgtccca gcacgacctc tatcagctgc gcgcacgaat tctgacgctg gggatcgtcg
      361 cggccgtggg gcttctgttg ttcatggctg tcctttcctt cccgtctttc acgacggccg
      421 aaggcatcct ctggcccgga gacgaggccc agattcgcac cggtgccgag ggcctggtcc
      481 gcgaaatcgc cgcggtgccc ggaacgcacg taaaaacggg gcaaatcctg atccgatgcg
      541 agaattccga gctggtgttc caggtgaaga tccttgaggc ggaacgtcgg gaatacgaag
      601 cgcgttacag gaatgcattc gccagggacc gcaacgagga gaggatcatg cgggaggaaa
      661 tcgcccgtat tgacgaggcg atctacaagg cgagggaaga actgtcggcg cttgatgttc
      721 gcagtcccgc ggagggcatc ctgattctgc ctcaggcgga agatctgccc ggacgattca
      781 tcaaacgcgg tgaggccttg ggttatgtgg tcgattacaa caagatcaat gtccgcgtgg
      841 tcatccccca gaccgatgtc gatcgtgtca gaaacaatgt ccgttcggtc cagttgcggt
      901 tggccgagac accgtccgtc gaactgacat cagagatcat ccgggaagtc ccggccgcat
      961 cgagccatct tcccagcctg gctctttcac tggagggcgg gggcgccatt gcgctcgatc
     1021 ccacggccga tgaaggatcg aaatcgttcg aaaattattt tcatttcgac ctccgaatgc
     1081 cggagagaag tcaggtgcgg atcggtgagc gggtctacgt gagattcgaa catgccccgg
     1141 aatcgctcgc acgcaggggg tacagaacga tgcggcatct ctttctgaga accttcgacc
     1201 tttgatgcgg aattgcgaaa gcggcagggt gaaagatgga tgacaggttc ggcaaagcag
     1261 gacacccccc cggaaaaggc tgatccatga tgcgggatgt cgggacctgg ccgcccgcgg
     1321 attatttcgt tcggccggag cgacggggac acaggagaac gcttctcgac cgcatggcca
     1381 gagaactagc gggttatgtg atccgacccg caagggtccg ccttctcaac ccggatcgga
     1441 tcgtcgcggg aatcaggcgc cttgaggagg cgcttcgcgt caaaacagac ggcgaactca
     1501 aggatttggc cgattccatg aggcggcggc tccgttcgga aggattcaaa gaccccgttg
     1561 tgacggaagc ctttgcattg atccgggagg ttgcaggccg aacggtgggg atgagacatt
     1621 tcgactgcca gttgatcggc ggcgcggccc tgttgaaagg cttcctggtg gaaatggaca
     1681 cgggcgaagg gaaaacgctc gtggcaacgc tgcccgcggc gacggcagcc atggccggtt
     1741 taccggttca tgtgatcacg gtcaacgatt acctcacggc cagggatgcg gaatggatgg
     1801 gagagatcta ccggtttctc ggtcattccg tgggttgcat cacccatgag aagacgccgg
     1861 tccagcgccg acaggcctac cgcgccgata tcacctactg caccaacaag gaactcgttt
     1921 tcgattacct tcgggacagg ctcctgctcg gtgaagcctc agaccccctt cggctctacg
     1981 gtgagcatct ctacgatacg aagggaaaga gcgaccggct gctcctgcgt gggctccatt
     2041 acggtattgt cgacgaggcc gacagtgtcc tcgttgacga ggcccggacg ccgctcatcc
     2101 tctcaaagaa agagaacgcc ggtcgtgaag aggaagtcgc gcaacaggcc ttgaccgcgg
     2161 ccgcgaaact ggtggagggc gacgactatc gcctggagta cgagacggat cagggactga
     2221 gaaaggtcgt catcacggaa ggcggccggg agacgatccg ccggatcact ggacctttcg
     2281 gcatccagtg gcggggcgcc gtccggcggg aggaactggt gaagaaggcg ctcgcggcga
     2341 cccggttgtt caagcgcgac gagcattatc tggtgcggga cgggaaggtc cagatcgtcg
     2401 acgaattcac cggtcgggtc atgccggacc ggtcttggga ggggggactg caccagttga
     2461 tcgaagtgaa ggaggggtgt gaaatcaccg gactgcagga aacggcggcc cgcatcagct
     2521 atcaacgttt cttcatgaaa tatctcgggt tgtgcgggat gaccggtacg gccgaagagg
     2581 tccgccggga gctatggtcg gtctacgccc tcccgttcat ccgggttccg acgaataaac
     2641 cggtccggcg tcgttccgga ccggatcgcc tgttcgcttc agacgatgag aaatggcagg
     2701 cggtggtgcg gcgggtccgt gaaatccacg ataagggacg ccccgttctc gtggggacaa
     2761 ggaccgtggc cgcctccgag caccttggcg ccctgctggt ggaggagggg ctttcatttc
     2821 agattctgaa tgcaaaacag gatggggagg aggcaggcat cgtggaacgg gcgggtgagg
     2881 cgggccggat caccattgcc accaacatgg ccggacgggg cacggacatc aaactggctc
     2941 caggggttgc cgaaaagggg gggctccacg ttctgatcac ggagcgtcac gaagcggggc
     3001 gaatcgatcg gcagctggcc ggaaggtgcg gaagacaagg ggatccgggc agttatgagg
     3061 cctttctgtc tctccgcgac ccactgatgg aaaacgcatt cacgggtggc tgggggcagg
     3121 tggaaaggtt gttccgaaga tcgggctatg atataagacg gatcgcggga cggcggatga
     3181 tgattcgagc acagaagaag gtggaaaagg cccacgcggg ggttcggaaa agactgctga
     3241 agtatgacga gcagttgggg gacgccctgt ctttttcagg aaggacggat tgaatcgcat
     3301 gaagccgacc catagcatca tccctgttct gctcatctgc ctgtcgggcc tgttctgtct
     3361 gggcgccgcg ccgaccgagt ccctgaaggg tctcctcgaa cccagcgaga tcgtggagct
     3421 ggggagtcag acgcccggca tcatcgagga atttctggca gaccgggggg accgcgtcaa
     3481 gaagggtcag gtccttgttc ggctgcaggc ggcggtggaa aaggccacgg tggatttggc
     3541 cgtcgcccgt ctcgaattcg ggaaacggaa ggttcaacgg aatgaagagc tgtatcaaaa
     3601 acagctcatt tccattcacg aaaaagacga aatggaaacg gaagtcaaga tcgctgaatt
     3661 gcagctcagg gaagccatgg agaagctcag gctgcgaacc atcacgagtc ccatcgacgg
     3721 cgtggttgtg aaacggctgc gctcgccggg agaatacgtc ggggaagaac ccatcatgac
     3781 gctcgccagt gtcgatccca tcaacgtaga ggtcttcgtc cccgcgtcct ggcacaggag
     3841 aatccgaaag gggatgcagg tggaggtgag gcctgaagcg cccctcagtg gcgtctatcg
     3901 ggccaaagtc gcagtggtcg atgaagtcat cgacgcggcc agcgggacct tcggggtcag
     3961 ggcggagctg ccgaatccgg atcaccgggt caacgcgggg ctgaattgca cggttcgatt
     4021 tctcccgtag cgatccgccg cgccggatcc ggtgagccgt cgaggacaaa ccagggcgtt
     4081 cccgcaggga acccactcaa agaaaagggg agaagaaatg aaaaaggtta tcgtattggt
     4141 tgcaatgtgt gttttgttct gttcctgtgc ctcgtcggcc atcctcaaaa ccaacgagaa
     4201 cctgttgagg ctgaaaaccg ggatggccca ggacgaggcc atgacgatca tgggcacgcc
     4261 gaccttgagc gagctgtatg aagcggctga cggaaatcag gtgtctatcc tctattaccg
     4321 tacggagagg aaggaaacga ccgtcttgtc cgcgaaggat gaatgcaccc ccgtggtgtt
     4381 cacgaacggg aaactggtgg ggtgggggga caggctgacc gccgcggaca tcaacctcct
     4441 gaaggtcaaa acgaagtagg cctgcccctt agagacggag ggaacggacc catgccgcaa
     4501 gatcggtcag tgcgcgcgcc gcgtagcggt cgcctcggtc cggtttgcgg actcgcccgc
     4561 ccagaggggt caagagaccg aaattaacgt tcatcggctg gaaggcggtc gggtcggatg
     4621 ccgtcagaaa gcggatcaga gatcccaggg cggtcgtcgg cggcggggga agaaacgtct
     4681 tcccatccgc gtgacgggaa aggctcaacc cggcgatcag ccccatggcg gcggactcga
     4741 cataaccctc gacgccggtg atctgacccg cgaaaaaaat cctgtcgtct gttttcagct
     4801 gcagggtctc tctcagcacg gcgggcgcat tgagaaacgt gttgcggtgg atgctgccat
     4861 agcgcgtaaa ctccgcctgt tcaagccccg tgatcatcct gaagatccga cgctgctccg
     4921 gccaggtcag ttttgtctgg aagccgacca tgttgaagag ggtcgccgca aggttttcgt
     4981 ggcgaagctg aaccacggcg tagggctgcc ggccggtgcg cggatcggtg aggccgaccg
     5041 gcttcagggg gccgaaggtc agggtgcgcg gtcccctctc ggccaggact tcgattggca
     5101 ggcaaccctc gaagcatttc gtctcctcga aggtatgcag ggggaccttc tcgccggtca
     5161 gcagggcatt caggaacctt tcatattcct caagattgag cgggcagttc agatagtcgc
     5221 cgccgccctc ggggtcgtag cgggaggcgc ggaaaacccg ggttctgtcg atggactccc
     5281 ccgtcacgat cggcgcgatg gcatcataga aggccagatg cttggcgccc gtccgttcag
     5341 ccaggttggc ggcgagagcg tcggaggtca gcgggccggt ggcaatcaga acaaaaccct
     5401 cggaagggat ctcctgaact tcacggcgga caagggtgaa ccccggtttt cgctgcaggc
     5461 cgtcctctac ctgtcgcgag aaggcctccc ggtcgacggc gagcgcccgg ccggcgggaa
     5521 cggcggaggc atccgcggca gccaggatga gggagttaag cgcgcgcatc tccgccttga
     5581 ggagacccac ggcgttctgg atccggtcgg aacggaggga gttgctgcaa acgagttcag
     5641 cgaggagagg ggacgtgtgt gccggggaga agcgttcggg cttcatctcg acgagggtga
     5701 cacggtggcc cgccgccaga agctgccagg ccgcttcgca acccgcgagt cctccgccga
     5761 tgatggtgat gggcgttccg gctgacggca aggctttagg ctccccgact ccggttccta
     5821 tgggttcgac ggtttgtatt tgtggatggt cctgcattcc ggataaccgg tgcaacccag
     5881 aaattttccg aaacggccgg ttttgagcgc catcggcttg ccgcacgtgt cgcagagggt
     5941 gtcggtaatg acggcttcgg ccgcgggagc gccggccttt ccgccccggg gaatccggac
     6001 gatgtgcttg cattccggat agccggaaca ccccaaaaag tttccgtagc gaccccgctt
     6061 gacgaccatc ggtttgccgc atttttcaca gagatcggca ccctccggcg cctcggtcgg
     6121 ggaggtgatc gggtttccgt ccgggccgac attcatcgtg tgacggcatt cgggatagcc
     6181 tgagcacccc agaaattttc cgaaacgccc ctcgcgggca agcatcggtt tgccgcacag
     6241 cggacaggcg atgtccgtca cggcgtcccg cgccggctgg atgtcgccgt tgtcgtcctg
     6301 cgtgaagttt ttcgtattcc tgcagtcggg atagttcgtg caggcgagaa agcgtccgtt
     6361 ctttccccat ttgatcacca tcggcttgcc gcatttttcg cagacgaggt cggtcggaat
     6421 ctcctcgcgc ttgacgttgg tcatgttggc tttcgctttc atgagttcct ctgtgaacgg
     6481 tcgatagaaa ttgttcagga tgtccagccg cctggcctgt ccatcctcga cgcggtcgag
     6541 ctccgtctcc atccctgcgg tgaaggtgac gtccaggata cgcggaaagt tcttcacgag
     6601 aaggtcggtc acgagaatgc cgagttctgt cgggatgaac cgtcccttct cgaggcggac
     6661 gtactcgcgt tcctggatgg tgctcaggat ggttgcgtac gtgctgggac ggccgatgcc
     6721 gttttcctcg agttcccgga cgagcgaggc ctctgagaaa cggggcggcg gctgggtgaa
     6781 tttctgctca ggcttgagat cgaggagggc caggacttcc cctttgcgga cctcaggaag
     6841 aagcttgccg atgccgtttt catcgccgtt gtcttccttg ccctcggtgt acacgatcgt
     6901 gaagcccgga aatttcatca cggccccctg cgcgcgaaag aggcacgcgg cggccgtgat
     6961 gtccacggtg gtctgatcga ggacggcggg gttcatctgg gaggccagga agcggttcca
     7021 gatgagctgg tagagacgga attgatcgtt gctgaggaag ggcttgacgg tctggggcgg
     7081 atgggacatc gacgccgggc ggatggcctc gtgggcatcc tgggcctttc cggagacttt
     7141 gaacgtccgt gccttgggcg ggagaaaggt cggatcatag cttcgtcgga tgaattcccg
     7201 cgcatcggtc agcgcgtcgg cggcgatgcg gaccgaatcg gttctcatgt aggtgatcag
     7261 ccccaccgga ccctccgcgc cgagttcgat cccctcgtag agtttctggg ccgtggacat
     7321 ggttttcttg gccgaaaagc ggaaccagcg cgaggcctcc tgctggagtt tgctcgtcgt
     7381 gaagggcggg ggcggctggc gccggacctc cttcttctcc acggaggaaa cggtgaacgg
     7441 ggcctcccgt aggctgcgga cgatctcgtc ggatccttcg gcgttgacga ccttggcctt
     7501 tttcccgtcg atcttgatca gcttggcttc gaagggcggc ggattcgttc cctggaccaa
     7561 agcggtcagg gtccagtatt cctcggccac aaacgccttg atctccgcct cgcggtcaca
     7621 gatgatgcgg acggcgacgg actggacgcg accggcggat agtccgcgtt tgaccttgtc
     7681 ccagagaatg gggctgattt ggtaacccac gaggcggtcg aggatgcggc gggtctgctg
     7741 cgcctcgtag cggtcgaaat cgagttcgcg ggggttgcgg agggcttcga ggatggtcgc
     7801 ccgggtcagg tcgttgaaca ggacgcgccg gaccgttttc ccgcgaccac cgatctcttc
     7861 ggcgatgtgc caggcgatgg cctctccctc gcggtccggg tcgggtgcca gaaggatctg
     7921 gtgtgccttg gccgccgctt ttttgagttc ggcgatcacc ttctttttgg cgtccatgac
     7981 gaggtaggtc ggctcgaagt ccttgtcggg atcgatcccc agacggttct tgggaagatc
     8041 cttcacgtgg ccgacggagg cccggacgtc gtattccggg cccagatatt ttcgtagggt
     8101 tttgaccttg gtgggagatt cgacaatgat cagcgattcc gacataatga ttcctcgcgc
     8161 agatatcggt ttccgggcat ttgccggacg agaccgttca gttccagttg caggagcgcc
     8221 gtgataattt cctggaccgt cagccccgtc gcatggacga gggcgttcgc atcgacggga
     8281 atggcggaaa gggcggagaa gacaatccgt tcccgttccg acagagattc catcgcggcg
     8341 ccgtccgacg tcgcggcgtc ggggatggca tccggggaag gttgggcggc gggaggcaca
     8401 atgcccgccc gctggagttg cggcaggatc tcttccagga tgtcatcgac gccttcgatg
     8461 agcttggccc cttccttcag gagccggtgg gtgccacggg agccggacga gtcgatggcc
     8521 ccggggacgg caaagacctc acgaccctgt tccagggcga tgcgggccgt gatgagcgat
     8581 ccgctcttca tgccggcttc caccacgacc acgcccaggg agaggccgct gatgatgcgg
     8641 ttgcgggccg ggaaattggc ggcgttcggt ggggttccga aaggatactc gctgatgacg
     8701 gcgccgctcg cggcgatctt acggtagagg gattcgttct ccggtggata gatgacgtcc
     8761 ggaccgctcc ccaggaccgc gatggttcgt ccgcgtccgg ccagcgcccc gctatgggcg
     8821 gcggagtcga ttccacgcgc catgccgctg acgaccgtga ttcccctcag ggcgagctcg
     8881 cgacagaggc gttccgtcgt gaagcggccg taaggactcg cctggcgcga tccgaccacg
     8941 gcgatgggga tatcctcgtt ggacaaggtt cccctcacgt agagcagagg cgggaaatcg
     9001 tagatgttaa gcagacgccg gggatagtcg gcgtcctgaa aggtgagcag ggtcatgctc
     9061 ccttgtcgaa cgcgctccag ttccttctcg caggccttcc agtcgtcgaa cgacttgatc
     9121 tgtgcggcgg tcttgtctcc gatgccgggg atgcgtttca gcgtttcgta tgacgccgcg
     9181 aacaccttgg ccggcgaacc gagcgcctcc acgagggttt tgaaggcgag attccccacc
     9241 ccttcgactt ttttcagagc cagccaatcc ggcagcgatt ctttttgcat gggaaacgtt
     9301 cctgaattct gaaaggcggc aaacatatat cggacccgca cggggtgtca agtcttttcc
     9361 ccggaaacct ctcaatcgcg aaataaccat tgcctccgga gaggctttgg tttaaggaaa
     9421 ggctatttca agatgatgag gtgaacatga agcgatcatc cggccttgtc gcgaggatcc
     9481 tcgttgcgac gattctggcc gtcccgttga cataccggcc cttgatcctg tcggcccaga
     9541 cgacgccggc accggcgccg gtgatcaacg tcgctcccca cgaaatcgat ctcggaggtc
     9601 tcacgtcggg acagaccctg aaagggaaat tcatcctgag ttatcccggg cccgcttccc
     9661 aggcgtggaa tacgcagacc ctgtcgggct ggaccttcct ggaccaggat cggctgtccg
     9721 gaacagtcgc cgaggagccg tgcgtcctgg aggtgaggct ctcccgattc gacgacggag
     9781 aggaacggga cgttgatgcc caggacgcgg cgatcagaag cgtacggctg tcgctggaga
     9841 gcgggggaaa ggcaatcacg gcgcgcaggg aattctccgc cggaccgcac agggtggcga
     9901 ttcccgtttc gctgccgggt ggcgtccgga atctccatat tcgcttcagt ctgccctatc
     9961 cggagctcga aacgttcctt accgtcgatc ccccccgtct cgattttggg agcctgggcc
    10021 ccggagaaac ctcgacacga atcatccgcc tcaacaatgt cgggcgcgag cgcctgcact
    10081 ggaaggccgt cgggcccgcg ccggagaagg aggtcgtccc cgtcagcagg ggacgctacc
    10141 actcctttca gaatccggag cttcgcggac agggaacgta tcaaccgcca gtgaggatcg
    10201 cggaggcggt ggaatttccc ccgggatggt ccgaagaggg ggggtatcct gtttcccatc
    10261 cgcagggatc aaacatgaaa tgccgcttca aggggacggg tgtcgtattg catctctgga
    10321 aatcatccga cggcggaagc ctgtcgattt atatcgacaa tcggctcgtt gaaaccgtca
    10381 acgcgatatc cgatccggtc gagaggttca cctcgaacgg cgtttcgggt ctcaacgaag
    10441 gacctcacga gctgagcgtg gtcgtcaaga gcggacgtct gaaactggag gggatgacgg
    10501 tttacgacca ggatgtccgg cgcggacctg ccgactggat caaccttttt cccgattccg
    10561 gcgtcatcac gcgtgagacg gatttcgtca acatcgctct ccgcgctcag aacgtcccac
    10621 ccggaaccta tcgggatcac cttctgatcc gctccacggg tggggatcag gtcgtcgacc
    10681 tctccgtgga ggtcagggca gacgcaccca cgaaaatcat cgacgtctac cggtattttc
    10741 gggacaacga ttgtctgttc accgtcaatc ccgaggccga agtgcagcgt ctccattcgg
    10801 gcggatatca gcgccagggg gttgcattcc ggcttttcat tcccggaata ccggggacaa
    10861 cggagctcta tcgctggttc aacccgaaga agcgggacca ctattacgca atcaaccggt
    10921 tggccgtcaa tccacagtcc ggatacgtcc tggagggagt catcggccat atcgcgacct
    10981 cgcggatgaa caacacacgc ccgctctatc gctggcgaaa cccccggacc ggtcatcatt
    11041 tcttcacaac cgatcagagc ggggaaggtc gggacaggaa gggtttccgc ttcgaaggaa
    11101 tcgtcggtta tgtacgctga caaacaccgc ttttacactt gacaaaagcc ggcaaaggcg
    11161 ttaataggcg tttccttttt ggaacaacag gcgccagtag ctcagctgga tagagcaacg
    11221 gacttctaat ccgtaggtcg cgggttcgaa tccctcctgg cgtaccagga atgcatggtg
    11281 ggtgtagctc aaatggttag agtgccgggt tgtggccccg gaggttgagg gttcaagtcc
    11341 cttcactcac cccacacttg ccagttggga cagaatgaga tataatgcgc ccgtagctca
    11401 gttggataga gtcgcagact tcgaatctgt tggtcgtacg ttcgagtcgt accgggcgcg
    11461 ccagattgga gagggataag acaaacccgt ccatgtgggc ccttagctca gccggtagag
    11521 caactgactc ttaatcagta ggttgtcggt tcgatcccga cagggctcac cagatttcaa
    11581 ggggttaggc agtatgccga gccccttttt ttctttggac cccgaagcat ggaaaaaaaa
    11641 cgcctctata tccggacctt cggatgccag atgaacgtgc atgattccga gcagcttgcc
    11701 gcgctccttg cttacgaagg ctatgagcgc gccgatcggc ccgaggaggc cgatctgatc
    11761 gtcgtcaaca cgtgcagcat ccgcgagaag gccgcccaga aggtttacag cgaactcggg
    11821 cggctccgga gcctgaaatc cctgaagccc gggctgcgga tcgcggtcgg cggctgtgtt
    11881 gcacagcagc gggggcgggc gcttctggtg gcggcgcccc atgtggatct ggttttcgga
    11941 acgcaccaac tggcgagcgt tcccaggctc ttgaacctct gcggtcacgg tggggcaggc
    12001 gtcgtggaaa cggcgttttg cgaccgggtc gaatcgttgg cgatcttcgc cccgccccca
    12061 cacggaacgg tgagcgctta cgtgaccatc atgcaggggt gcaataattt ctgtgcatac
    12121 tgtgtcgtcc cctatctgag ggggcgggaa caaagccgtt ccggtcggga gatcatagcc
    12181 gagatccgct ccttggcgga tctcggcata cgcgaggtga cgctgctcgg tcagaacgtg
    12241 aactcctacg gagtggggac gccggaaggg atggatttcc cgggcctgat tcgggaaatc
    12301 ggcgccgtct ccggtatcga gcgcatccgc ttcacgacct cgcatccgaa ggacctgtcg
    12361 gcggacttga tgcagtgctt ccgagaggtc gagtcactct gcggacacat tcaccttccg
    12421 gttcaggccg gttcgaaccg ggttctcgtc cggatgaacc gggggtatac ccgggaggga
    12481 tatctggaca aggtccaacg actccgggac gcctgtccga acatcagcat cacctcggac
    12541 atcatcgtcg gtttcccggg ggaaaccgag gccgattttc gtgagacgct ggatctgatg
    12601 ggaaaaatca gatttgacaa tttgttctct tttaaatatt ctcgacgcga aggaacggcg
    12661 gccatgaaga tgacggagcc ggttgcggag gaggtcaagt cggagaggct gaagatcctg
    12721 caggcccttc aggacgaaca tacccaggag agaaaccggc tttcgatcgg aagggtagag
    12781 gagatcctcg tggaaggcgt cagtcgggca tcgtcccggc atctaacggg gcgaacccgc
    12841 ggcaacaaga tcgttaactt tgccggagaa gaacggctga tcggccggac ggtgcgctgt
    12901 cgcatcacgg aggcctttct ccattccctc cggggcgagc ttcaacttcc ggaggcctga
    12961 tcatgctgat cgaaatgaag atatccgggc tgacgatcga tcccctcacc aacacgccga
    13021 tcgttatcct taaggacctg gaagagaaga aggcgatccc gatctggatc ggcttgtttg
    13081 aagcgagtgc gattgccact gagatggaaa aaatcagctt cgcccgtccc atgacgcacg
    13141 acctgctcaa cgagattctg aaaacgctcg atgtccaggt cacgcgggtg gagatcaacg
    13201 acctgaggaa caacacattc ttcgccaaca ttttcgtggt cagggagggg agggactacg
    13261 tcatcgattc ccgcccgagc gacgcgctgg ccctcgccct gcgcgcacgc gccccgatct
    13321 ttgtcgagca gaaggtgatc gacaagtccc gaaacgtgga tttcggatcc aagggaacgg
    13381 accaggatca gttgcaggaa gaaaaattga aggaatttct ggagaatatg tctccggagg
    13441 agttcggcaa gtacaagatg taggcctctg tgcgcggtgc cacgaaagac gggtcgaagc
    13501 ggactttcgt ggctattgtt tttatcggac ccctgatggt cccggatcgc acggcatctt
    13561 taggggaagg ccggaacggc gggatgacga catgatcgag gacgtggagc aggccctgcg
    13621 tcgtttctcg cgacacatgg aaatggagcg ggatctttcc ccccataccc gatcgaacta
    13681 cctgcacgac gttcggcagt tcgtcgcgta tctcgaggcc gaagccctga tcgatgcggc
    13741 gaaggggcag ggactggaat ccatcgatgc cctgatgatc cgaactttcc tggccgggct
    13801 ttatcagcgc cggattcgga aggtttcgat cgcccggaag atatccgccc tgaagagctt
    13861 tttccgctac ctccagcgcg acggggtgct caagggcaat ccggcggaat tgatccagac
    13921 gccgcgcatc gagacgtatg tcccctccgt cctgtccgcc gacgagatgg aggaccttct
    13981 caaggcaccg caagatgccc ggttttctgg tgcgcgggat cgggcagtcc tggaactgct
    14041 gtactcgtcg ggcatccgtc tgagcgaact gacgggactc aacgtcgggg atgtcgattt
    14101 cgcggctggg ctggtcaggg tccgcgggaa ggggaaaaag gaccggatcg ttccggtcgg
    14161 aggcccggcg ctaaatgccc tgcgcgtcta tctggaggcg cgggatgccc tgtttccgga
    14221 gccggacggg gtcggccagc agccgatctt cgtgaaccgg cggggaggac gcatcagcac
    14281 gcgctccatc gcccggatcg tcgaccggtc gaccgtccag ggcgggttgc gccgcaagat
    14341 cagcccccac acgttccggc acacgtttgc cacgcacatg ctcgacgctg gcgccgatct
    14401 gcgggcgatc caggaatgtc tgggccacga gagcctgtcg acgacgcaga aatacacggc
    14461 cgtgagcgtc gcacggctga tggaggtcta cgacaaggcc catccgaagg ccggaggagg
    14521 acacaagaaa ccatgattat gcatggaacg accatcgtcg ccgtacgaca caagggaaag
    14581 gtcacggtgg ccggggacgg gcaggtcacc atcgacgtga cggttatgaa gcacggcgcc
    14641 cggaaggtcc ggcgcctcta ccacaacgag gtcattgtgg gattcgccgg agcaacggcg
    14701 gacgccttca cgctgttcga ccgcttcgac cagaaactgg agcagtacaa cggcaatctc
    14761 ctgcgcgccg cggtggaact gacgaaggac tggcggacgg atcgcgtcct ccggcacctg
    14821 gaggcgttga tgatcgccgt caaccgggaa agttttctca tgatctcggg catcggcgac
    14881 gtcatcgaat cggatgacaa cgtcatggcc atcgggtccg gaggccctta cgccctggcg
    14941 gcggcacgtg cgctcatccg gcataccgac ctcacggccg ccgagattgc ccgtgaggcc
    15001 gtctccatcg cctcggagat ctgcatctac acgaacgaca acatcacgct agaagaattg
    15061 gatacggcgg aggttccgga agggacgagc cgaagccggt cagggagcta gaggaataca
    15121 tgtcggagaa cggattgacc cccaaacgga ttgtcgaaaa actcgaccgg tacatcatcg
    15181 gtcagggcga ggccaagcgg gccgtcgcga tcgccttgcg caaccgctgg cggcgccaga
    15241 atgtcgtcaa ggaactggct gacgagattt cgccgaagaa tatcatcatg atcggtccga
    15301 cgggcgtggg caagacggag atcgccaggc ggctcgcgaa gttggacaat tcacccttca
    15361 tcaaggtgga ggcctcgaag ttcacggagg tcggctatgt cggccgcgac gtcgagtcga
    15421 tggtgcggga tctggtcgaa ctggccgtca acatgatcaa gggtgaagag cgggaaaaag
    15481 ttcgcgccag ggcgtccgag ctcgctgagg agcgtctgct ggacgttctg ctgcccgcaa
    15541 ggcccgagga gaggcgcgcg gaagacctca tcgcgggaca cccggggacg agtccggacg
    15601 cggcacccgc cgtggacacg acgcgggaga aactgcggcg tctgctccgc gaagggaaac
    15661 tggatcagcg accggtggac ctggaggttt cggaagcgcg cacgagcccc atgatcgagg
    15721 tgttttcttc aacgggcata gaagacctcg gcctcaacat caaggaggtc ttcggaaata
    15781 tctttcctca aaagaagaag cagcggcgcg tcaaggtgcc ggaggccatc gagattctga
    15841 ccgacgagga ggcccagcgt ctcgtcgaca tggacaaggt tacccgcgcc gcggtggagc
    15901 gcgtggagca atccggcatc atctttctcg acgagatcga caagatcgtg ggtggagacg
    15961 gcgcgcacgg accggacgtg tctcgcgaag gcgttcagcg ggatctcctg ccgattgtcg
    16021 agggctccac ggtcaacacg cgctacggga tggtcaagac ggatcatatc ctgtttatcg
    16081 ccgccggggc cttcagcagc accaagccgt ccgatctcat cccggagctt cagggacgtt
    16141 tccccatccg ggtcgagttg gattccctgg ggaaggagga gttcatccgc atcctgacgg
    16201 aaccgcacaa cgccctgatc aagcagtacc gggaaatgat ggcgacggag gggatcgata
    16261 tggtcttcac cgacgacgcc gtggccgaaa tcgccgaagt ggcggagacc gtcaacgagc
    16321 ggacggagaa catcggcgcg cgccggctgt acacgattat ggaaacgctc ctggacgagg
    16381 tgtccttcga tgcccccgac atggaggaaa agcaggtcgt tatcgacgcc gactacgtcc
    16441 gcgagcggct cgacgacatc gtcgaagacg aggatctgag ccgatacatt ctttaggagg
    16501 ttgaccagtg gtcgatccga tacagcagtc catggcgcgg gcggatgtcc ttcttgaagc
    16561 cctcccgtat atccggcggt tctacaacaa gaccatcgtg atcaagtacg gcggtcacgc
    16621 catggccgac gacgccctga agaatgcctt tgcccgcgac atcgtgatga tgaaatatat
    16681 cgggatcaat ccggtggtcg ttcacggcgg tgggccgcag atcggctcgt tcctgaagaa
    16741 gctcggaaag gattccacct tcatccaggg catgcgggtg accgacgagg agaccatgga
    16801 cattgtcgaa atggtccttg tggggaaggt caacaaggaa atcgtgggac tcatcaacca
    16861 gcacggcggg cgggcggttg gcttgagcgg aaaggacggc gggctcatcc gggcggaaaa
    16921 atacctgctc agcgccgaga aggcgaagga caccccgccg gagatcatcg atctggggct
    16981 ggtcgggaag gtgagggaga tcgacggcag cctgatttcc gttctcgtaa aggacggctt
    17041 tatccccgtc atcgcgccga ccggcgccgg cgagagcgga gagacctaca acatcaatgc
    17101 cgacatcgtc gccggggcgg tggcctcggc cctgaaggcg gaaaagctcg tcctgcttac
    17161 ggatgtcgcc ggcgtgctgg acgccgagaa gaagctggtc aacacgatga acaaccgggc
    17221 ggcccagcgg atgatcgaga ccgggattat cgagggcggc atgttcccga aggtcaaatg
    17281 ctgcctgaag gctttgcgtg gcggggtgca caagacccac atcgtcgacg ggcggttgcg
    17341 gcacgcgatc ctgctggaga tatttaccga ttccgggatc ggaaccgaga tcgtcgctta
    17401 gggatccttc aaaatcggga tccctagccg aacctgacat gttttcatga caaatttccc
    17461 gggtgcgttt ccgggatgcg gttctcgaag gtcgttgagc gggctggagg ggaggaacaa
    17521 gcgtgaataa aacggagtgg atggggctgg gcgagcggta cctgatgaac acctaccggc
    17581 gcgcgccact tgttttcgaa cggggtgaag gcgtacgact gtgggactgc gacggtcggg
    17641 agtacctaga ctttctggcg ggcatcgccg tctgcagcct cggacatgcc catccacgca
    17701 tcgtcgaggc catccggcgc caggcgggcc gcctgatgca tgtctccaat ctctaccaga
    17761 tcgagccgca gatcctcgcg gcgcaggccc tgtgcgaacg gtccttcgcc gaaaaagtct
    17821 ttttctgcaa cagcggcgcg gaggcgaacg aggcggcgat caagctcgcc cggaagttcg
    17881 gtcacgaacg cctgggcggg cggtatgaaa tcgtcaccat gatcaattcc tttcacggaa
    17941 ggaccctggc cacgatcacg gcgacgggcc agacgaagtt tcaggcgggg tttgaaccgc
    18001 tgccggccgg cttccgttat gcaccctacg acgacccgaa ggcgctcgcg gatgcggtca
    18061 cggacaggac atgcgcaatt ctcgtcgaac cgatccaggg ggagggcggg atccggcttc
    18121 cggcacccac ctatcttcag gaggttcgcg ctctgtgcga cgcgcgcggc attctcctga
    18181 tgcttgacga ggttcagacg gggatcggcc gcacggggac gctcttcgcc tacgagcagg
    18241 cggggattaa gccggacgtc atgaccctgg ccaaggccct gggcaacggg tttcccgtcg
    18301 gggcaatgct gtcgacggag gaggtcgccc gggtgttcga gccgggaacc catgcctcga
    18361 ccttcggcgg gaatcccctg gcgatggccg ccgtcctgtc ggttctcgaa gcgatcgatg
    18421 aagagggtct tcttaagaat tgccaggaga tgggcgaata tttccttcag aaactgagcg
    18481 ttctaaagag gaagcattcc ctgatccggg acatccgggg aagaggactt ctgatcgggt
    18541 gcgaactcgg ctgcgaaggt gcgccatacg tctcgcgatg catgcaggaa ggcttgctca
    18601 tcaactgcac gaacgccaac gtactgcgct tcgccccgcc gctcgtcgtg acggccgggg
    18661 acatcgatcg ggcggcggac attctcgacg ccgcattggg ggcatcatga aaaaggatct
    18721 tttgagcgag tttgatctgg ccaaagacga ctttgagtgc attttcgaga aggctaaacg
    18781 tcagaagcgc attcacgaag aggggagatt ctaccagccc ctccggggga aaacgctggg
    18841 gatgatcttc gacaaatcgt cgacccggac gcggatctca ttcgaggtcg ggatgtacca
    18901 actgggcggg atcgccctgt ttctgaacag ccgcgatacg cagcttgggc ggggcgagaa
    18961 tatcatggat tcggcccgca tcatgtcccg ctatctcgac gggatcatga tccggacctt
    19021 ttcccagaaa gccgtcgagg agttcgcgcg ccatgcgtcc attcccgtca tcaacggtct
    19081 gaccgacctc ctccacccct gtcagatcct gagcgatctg ttcacgatca tggagaaaaa
    19141 gggcgcctat gagggaaaga cgatcgccta catcggcgac gggaacaaca tcgccaactc
    19201 ctggatcaat gcggcggccc ggctcccgat tcgcctggcc ctggcctgtc cggagggcta
    19261 tcgtccggat aggaggatcc tggcccgagc gctgaaaaac aacggcgccg acatccttct
    19321 gacggaccgt cccgaggagg cggtcaaggg cgccgacatc gtctacaccg acgtctgggc
    19381 gagcatgggg caggaacacg agcaggagga gcgggccggt cgttttcaga ggtttcaggt
    19441 caacgaaagg ctgctcaaac acgcccggaa ggacgccctc gtcatgcact gcctgcctgc
    19501 ccatcgcggc gaagagatca cggcggaggt cctcgacgga ccccagtcca tcgtcgtcga
    19561 tcaggcggaa aaccggctac acgtccagaa agccattctt gagatactac tttaggggag
    19621 gtttggtaat gtctgataat attaagaaag tcgttcttgc ctattccggg gggctggata
    19681 cgtccgtcat cgtccggtgg ctgatcgaga cctatggatg cgaggtcgtc tgcttcgccg
    19741 cggacgtcgg tcaaaaggag gaactcgacg ggctcgaggc caaggcgatc aacaccgggg
    19801 cgagcaagtg ctacatcgag gatctccggg aggaattcgt ccgggatttc gtgttccccg
    19861 ccctgcgggc caacgccatc tacgagggga cctatctcct ggggacctcg ctcgcccgtc
    19921 cactcatcgc caaacgtcag atcgaaatcg cccacgcgga aggcgccgac gccgtctgtc
    19981 acggcgcaac cgggaaaggg aacgaccagg tccggttcga gctcacctat atcgccctgg
    20041 atccggccat ccgcatcatt tcctcctgga aggatcccca ctggacgttc aggtcacgcc
    20101 ccgacatgat cgattatgcg gagaaatatg gaattccgct gcccctgtcg aaggaaaaac
    20161 cctacagtgc cgatcgcaat ctcctgcaca tgtcgcatga ggggggcatt ctggaggatc
    20221 cctgggcgga gccgccggag gacgtcttcg tcctgacggt gtcgccggag gatgcgccgg
    20281 accggccgac ctacgtggaa atcgggttcg agcgggggaa tccggtcgcc gtggacggcg
    20341 tggcgatgac gcccgcggcg ctcctcgatc ggctgaacga cctggccggc gcgaacgggg
    20401 tcggccgcgt ggacatggtc gaaaatcgct ttgtcgggat gaaatcccgg ggcgtctacg
    20461 agacccccgg cggcaccctc ctctgggcgg cacaccgcgc cctcgaatcc atcacgatgg
    20521 accgcgaggt catgctgatg cgagactccc tgacgcccaa gtacgcgcag ctcgcgtaca
    20581 acggcttctg gtacgcgccc gagatggaaa cactgcagcg gtacatcgac gcgacacagg
    20641 agaacgtgac cggcacggtg cgcgtgaagc tgtacaaggg caactgcatc gtggtcggcc
    20701 gcaagtctcc ccactccacc taccgggagg atttcgccac cttcgaccaa gaccaggttt
    20761 acaaccagat ggacgccacc ggtttcatcc gtctcaatgc cctgagactg aggatccgat
    20821 cgctgatcaa gggccggagc gcctgatgca ttcgtcatcc gtcagaaaga gcggcagatc
    20881 atgataccga taaacaagac gaaaaaactc tggggaggcc ggttcaagga gcccaccgac
    20941 cggctggtcg aggcctttac cgcctcagtt cagtatgacc ggcggctgta ccgctacgac
    21001 atcgagggga gtatcgccca cacccgcatg ctggtcaagc aggggatcct gaagaagaaa
    21061 gaaggcaccc tcatcatcac ggggctgaag aagatcctca aggacattca ggaggagacg
    21121 ttcaccttcc gcaccgggga cgaggacatc cacatggtca tcgagcgggc cctgatccgg
    21181 gcggtcggcg acgtcggcgg caaggtccat acggggcgca gccgcaacga ccagatcgcc
    21241 ctggatatcc ggctctacct ccgggacgag atccgggagg tcctcgtccg gatcgccgcg
    21301 ctcaaggaca cgctcgtcga cttggcgaag gaggaattcg gcacgatcat gcccggctat
    21361 acgcacctac agaaagccca gccggttctg ctgtcgcatt atctgcttgc gtactgggaa
    21421 atgctcgacc gggacgaaca gagactcgcc gattccctga agcgggtgaa cgttctgcct
    21481 ctgggcgccg cggccctggc cgggacgggc ctgcccctgg accgacaata cacggcggag
    21541 ttgctcggtt tcgaaagcgt ttccagaaat agcatggata ccgtcggcga tcgcgatttc
    21601 gtggccgaat tcatttttac ggcctccgtc gtcatgatgc acctgagccg attctgtgag
    21661 gacctgatcc tctggtccac ggaggagttc ggttttatcg aaatcgccga cgcctatacg
    21721 accggcagca gcatcatgcc tcagaagaag aatccggacg tcgcggaact cgtccgggga
    21781 aagaccggac gcgtctacgg ccatctggtc accctcctga ccctcctcaa ggggcttccc
    21841 atgagctaca atcgagacct ccaggaggac aaggagccgc tcttcgactc ggtggacacc
    21901 gtcaaggcct gcctggacgt cttcaccgcc atgctcaggc atacccgttt caaccgggaa
    21961 cggatgctga aggaagcgga ccgtggcttt gccgtcgcga ccgacgtcgc ggagtacctc
    22021 acgcggaaag gtatcccctt ccgagaagcc caccggatcg tcgggaggat tgtcgggtac
    22081 tgcctggaga acgacaaatc cttcgacgat ctgacactga aggagtggcg gaagttcgac
    22141 cggaaattca cccaggacat ccaggacgcc ctggcggtcg aaacctccgt cgacgcccgc
    22201 atgacggccg gaggcaccgc ccgggagctg gtcaggaagc ggatcgagga gatcgaacgt
    22261 caacgtgaac cgaaagcctg accgaaacgg cgccagccgt tggattcttg cgatccttat
    22321 cgtcatcctt gccgtcacgg tcctgtgggc ctgcggcagg aaggcggatc ccctgtcgcc
    22381 acgggtcgag gcgcccgcgg ccatcggtgg tttgacggtg gagagattcc ccgagggatt
    22441 ccggctagga tgggcgccgc ccggcgccgg ggtggcccgg atcaggatcc tgcggagcga
    22501 cgtcacagac gcctcctgtc ccggctgccc gaaggagtcc cggctgatca cggaactgat
    22561 tcccgatgac gacggagtgc tgcctgtgac ctacacggac cggagcgtca aggaaggcgc
    22621 tgactatacc tatcggctta tcgcctgcgg cgcggacggc gtctgcggcg acgagagcgc
    22681 ggagaccgga atcaaggact gagggccgtg catgaatgac ttcaaattca ggcagaatcg
    22741 cctctattgt gaggacacgc cggtggagga gatcgcgggg gcggtcggca ccccgtttta
    22801 cctctacagt ttccgcacgc tgacccggca ttttcaggtc ttcgacgagg cgtttgccga
    22861 cgttcctcat ctggtctgct acgccgtcaa ggcgaactcg aatatcgccc ttctcggtat
    22921 ttttgcccgg gccgggggag gggcggacat cgtatcgggc ggcgagctgt accgcgcgct
    22981 caaggcgggc gttccccccg ccaagatcgt ctattcgggg gtgggaaagc ggatcgatga
    23041 aattgagtac gcccttcgcg agggcatcct gatgttcaac gtcgaatccc cgcaagaact
    23101 cgaattgatc gacgagacgg cggcacggat ggggaagcgg accggtatcg cgctccgggt
    23161 caacccggac gtcgatccgg agacccaccc gcacatttcg acggggctca aggagaacaa
    23221 gttcgggata gatgtctccc gttcgctgga ggcctaccgc cgggcaaagc gtctctccag
    23281 ccttgacatc atgggcgtaa gctgccatat cggatcccag gttacgaaga catccccctt
    23341 cgtcgaagcc ctggagcgga tcaggcggct gatcgttctg ctccgggaag aggggatcgc
    23401 gatccgctac ctcgatctgg gcggcggcct aggcattacg tacgaccggg aaacaccgcc
    23461 gcatccgacg gagtatgccc gggcgctgct cgaagcctcc cgcgagatcg actgcacctt
    23521 tatcttggaa ccgggtcgcg tgatcgtcgg caatgcgggc attctggtga cccgtgtcct
    23581 ctacaccaag gagacggagg ataagcgctt cctgatcgtc gacgccggca tgaacgatct
    23641 cgtccgcccg agcctctatc aggcctacca cgagatccgg cccgtcgttg acgaagatcg
    23701 cgaagagcgc gtctatgatg ttgtcggccc gatctgtgag tccggcgact atctggcaaa
    23761 gggacggtcg cttccggacc agaagcgggg cgaactgctg gccgtgatga gcgcgggggc
    23821 gtatggcttc gcgatggcct cgaattacaa ctcccgtccg cgcgtccc
//
LOCUS       DAOOLJ010000025        23068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2135_length_23068_cov_17.924955, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000025 DAOOLJ010000000
VERSION     DAOOLJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 23068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23068
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_23068_cov_17.924955"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLW16_05895"
     CDS             complement(<1..207)
                     /locus_tag="PLW16_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70470.1"
                     /translation="MNHFKYFSSLVVVLVLALALAGCAKPPEAEKSAADAAMNAAVAA
                     GAETYAAADLDAARKILDGAEAQMK"
     gene            complement(349..1113)
                     /locus_tag="PLW16_05900"
     CDS             complement(349..1113)
                     /locus_tag="PLW16_05900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70471.1"
                     /translation="MGVMEQFNLAQRVVIVTGGSKGLGREMALGLAEAGARVAVVSRT
                     RSLIEQTAAEIVGQGGEAIAVPADMTREEEVESMTAEVAKAYGRIDVLFNNAGIGGSK
                     RSVDLTVADWEHLMNTNARSVFLACRAVGRVMIRQRQGKIINIGSILGHGALPNSLHY
                     GASKAAVHHMTKTLALEWARFNIQVNCIVPGWFLTEMTKDQQEGENETFLLGKIPFRR
                     FGRPNEIVGLAVYLASGASDYMTGQTLFLDGGYAIS"
     gene            1466..3166
                     /locus_tag="PLW16_05905"
     CDS             1466..3166
                     /locus_tag="PLW16_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPB70472.1"
                     /translation="MKFNPAVVSYFLQRESTKRNIRQLLKFLFALAVLVSVYSVLFHF
                     LMAREGQNHSWITGFYWTLTVMSTLGFGDITFHSDLGRLFSMVVLVTGMIFLLTLLPF
                     TFIKFFYAPWIEAEARKRAPRELPPDTRGHVIITSYNPVTMALIEKLTDHRIDYVLIV
                     SDFKHALDLYDIGVRVAVGNVDDPDTYRRIRVEKAALVVAADRDEINTNIAFTVRELN
                     ERVPIIATADSPYSDDILRMAGSSKVLQIYDILGRSLAAWTVGGDCRANVLGRFDTLL
                     IAEFPATGTPLVGKTLVESGLRENFGVNVVGIWERGRFEIPRADSRIERTSVLVLAGS
                     EENLAAYDEVYAFYHICKLTADPVLIVGSGRVGDKIAERFQERGSPYLVIEKNPKRLR
                     DERHYVVGDAADIRTLERAWIDKAPAAVITTHDDDTNIYLTNYLRALRPDMQILSRAN
                     LDRNVSTLHRAGADFVMSYASLGANAIFNYLKNEDTWMLAEGLNIFRMRAPDALVGRN
                     LAHSGIRNLTDCSVVAIRNADGLSINPDPLTPIAADAELILIGTNEGERKFLQTFTPA
                     "
     gene            complement(3232..4788)
                     /locus_tag="PLW16_05910"
     CDS             complement(3232..4788)
                     /locus_tag="PLW16_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB70473.1"
                     /translation="MVLGDYFEKHGKTIPKDEAIVFKDRRIDYGAYDRETDRMAMGLL
                     KLGIRRGDRVGIYLPLWPETLFIYFGAAKIGAVGIPMSIRTTAPELKFFINDAEISVL
                     FLANGFMGADFMGNLDAVRKDLPKLRHVVSLDSEKEGVLLYETFLADPDSAALKEAEA
                     AVREDDPAIFIYTSGTTGVPKAAILTHRNVIAMTDAQLRTTGVVKGDSLLLNLPFSHA
                     GGAVMGVMSAVNAGNKIVLMDLFDPEETLKLIAAERCTALGQVPVMYAMELNHPNAGK
                     YDLSSMKIAVVSAQPCPSELILAFRQKLGITPRNAYGLTEVSGAVTYTHPDDGEAKLS
                     YSVGRPIPEVDLALKDEDGRIVPTGEAGEICVKGPVVMKGYWKRPEEDAKVFDADGFL
                     HTGDMGRFEDDGYLVIVGRKKEMYIRGGENVYPPEIEEAICRHPDVMLAAVVGRPHEK
                     WGEVGRAYIMPKPGTSPTPEGIKEFLKDKLSHFKLPEDVIIRPILPLTPLGKVKKLDL
                     YTEMKQEFGT"
     gene            complement(5037..6389)
                     /locus_tag="PLW16_05915"
     CDS             complement(5037..6389)
                     /locus_tag="PLW16_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70474.1"
                     /translation="MEKYDVIIIGAGIAGLGVGGILQNKGFKTAVFEKSKVVGGRSRT
                     FELPGGWRVDMGTHCVDQGVHSSCAKLLTELGTDIPWSHNLEGFMFYGEDGKWKPMME
                     SFNLGEAERKELSDLQAWIKNASDEMIDHYDNVSLTKLLEERVKSPKIGEFCKTVGMV
                     QTTLTESDIISAGEFIHIYRDQMRVSKSPDLPFDAVRMPLGGVQTMMNAMAEAYKKRG
                     GTLKLGTPVRKVLVGKSGPTELVTDGGNYSAPIVVIAAPIWNVLRFLDMAAMDQLAPA
                     WAKRMRECEFETSASMGYTIGTKVPLFDDKCYLSAWRVPGLGMPLQILGHTNFDDTIA
                     PPGHMIAFMGACGTPAQVGDAAFRKKTLDQFWVVLNRMFPNMESNLVWKQDGFTIGID
                     GLSRSPGMTGRYRLPVELSEVPGLYFSGDCYLGRGVGMNTAASSAVICVDAILSKHGK
                     "
     gene            complement(6403..7962)
                     /locus_tag="PLW16_05920"
     CDS             complement(6403..7962)
                     /locus_tag="PLW16_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB70475.1"
                     /translation="MVLGDFFKKHSQEIPNNEALVFKERRITFKQYDEATDRVAMGLL
                     KLGVQRGDRIAIYGPLWPEVVFAYFGAAKIGAIGVPMSARTTAPELKFFVNDSESSVL
                     FMPSTFMGGDFMANLDAVREEMPTLKHVVCFDLEKEGVLLWEKFLTDPDPAVLNKAKD
                     AVQEDDSALFIYTSGTTGVPKAAQLTHKNLIALTNAQIEATGYHRGHIIVLNLPFSHV
                     GGAVMGIICNVNVGNKVVMMDAFNPEETLRLVSEEKATTLGQVPAMYAMELSHPNLSK
                     YDISTLKLPIVSSQPCPSELISAFKKIVGVIPLNAYGLTETTAAITYTHPSHGEEKLT
                     YSVGKPVPSIQMVLKDDDGNIMPQGEAGEICVKGPVVMKGYWKRPEEDAKVFDADGFL
                     HTGDMGRFEEDGSLVIVGRKKEMYIRGGENVYPPQIEDAICKHPDVMLAAVIGRPHEK
                     WGEVGRAYIMPKPGKNPTPEEIKEFLKDKLSNFKIPEDVIIRPILPLTPLGKVKKLDL
                     YTEMKQELGKK"
     gene            complement(8299..9516)
                     /locus_tag="PLW16_05925"
     CDS             complement(8299..9516)
                     /locus_tag="PLW16_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB70476.1"
                     /translation="MEKKKQQTRSRILDAAEALILESSIEEVTIAKIAERAQVADSLA
                     YKYFKGKNDIIFSVVAERTREETALLREHLQGIIEPKSRLSKMIWHMLKYNDSHPNHV
                     TNLLFRCRSNKDFYRSEAYRLMKEYEAILLNILEEGAREGVFRSDIDMKLMKHIIFGT
                     IDIEALSSIVTGEIEKSCTDLDDIMALLLPMLTTKGKPAVTNRQQAILLAAEKTFARQ
                     GYADAKIAAIAEEAQVAEGTIYEYFSSKEDLLFALSDRNLNAYLERVSNIFNPQAGPS
                     PPEKLWRLMRSFGLSFLSIRDFMTVYFMYTLFNRRYYETAIQPKQNEFARMMGQVIEE
                     GKQEGTFRADVNPRVFWYMFLGSYTHLALRWIIIDHKEFDKYTEVRHLMTLMMTAALR
                     EPPNVEYLVRIEF"
     gene            complement(9570..12002)
                     /locus_tag="PLW16_05930"
     CDS             complement(9570..12002)
                     /locus_tag="PLW16_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPB70477.1"
                     /translation="MSRRKTFRGLWRDGLAILDGGTGTELQRVGMPAGVSPEAWCLAH
                     PDTLSGIHRAYLDAGAAILYTCTFGGSRPKLAPYGHTDVHAINRDLARLARRAAGRRA
                     LVAGDIGATGRFVEPFGDLPFDEAVAIFREQAAGLLEGGVDLFVIETMLDIQEARAAL
                     IAVKEICRLPVMVTMTYEADGRTLNGTDPVTALITLQALGADAVGCNCSTGPEAMVPL
                     IAAMKPYATVPLMAKPNAGMPRLVGDKTLFDMAPEAFGAAARELAAAGANLIGGCCGT
                     TAAHIAALRTTLDGVAPQPPRRRSLAAISSARAHRIFAPGDPLVVIGERLNPTGKKAL
                     QQELREGKLALVRRMAREQEERGADLLDVNVGVPDIDEVETIRAVVRLLAAATPLPLC
                     LDSARVDTLAAALRLYPGRALINSISGESEKMAELLPLAARYGAMFILLPLAGGEVPA
                     TAEKRIQIVRDVYRAARRHGFTKEDIVVDGLVMTVASNPRAAAETLKTVAWSAERFGA
                     RTVLGLSNVSFGMPERDRLNSAFLTMAQAAGLTMAIANPSSDVLADARRAGDVLLGRD
                     RDAAAYIAHATQTAPSVEPVPPAADPGSPEERVREAILDGNREEIVARVDAALAAGIP
                     ADRLVDDCLIPAIVEVGDRYDKKTYFLPQLIAAAEAMKAALARLEPLLRREGTARAHR
                     GTVLMATVAGDIHDIGKNIVVLMLRNHGFDIVDLGKDVTAEAIVAAAQEHRPEVIGLS
                     ALMTTTMVRMKDVIARAADAGLAAKFMVGGAVVTAGFAASIGASYARDGVEAVRVATA
                     LVGEVRSAQPVM"
     gene            complement(12404..13141)
                     /locus_tag="PLW16_05935"
     CDS             complement(12404..13141)
                     /locus_tag="PLW16_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPB70478.1"
                     /translation="MNRILRKIIEIDEALCDGCGQCVTGCAEGALQIVDGKARVVSET
                     FCDGLGACIGECPTGALRIVEREAPPFDEAAAQRHLAEREKAVLPCGCPSTHVQLFAS
                     PAACEAANRPAQIGRGPEEESALTHWPIQIRLIPANAPFLKGADLLVLADCTAVAFPT
                     LHRDWLKGKVVMMGCPKFDDVDAYVEKFTEIFKTAGVRSVTTAVMEVPCCAGLPAIVR
                     KGMERAGVTIPLNEVVLGVRGKVLARR"
     gene            complement(13162..14796)
                     /gene="hcp"
                     /locus_tag="PLW16_05940"
                     /gene_synonym="priS"
     CDS             complement(13162..14796)
                     /gene="hcp"
                     /locus_tag="PLW16_05940"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPB70479.1"
                     /translation="MFCFQCQETVKNEGCGVRGVCGKTEETANLQDLLIHVLKGIALY
                     AEKAGQKGPLDRRYGRFVAEALFATITNVNFDNDRITALIREALALRDALKEICGADA
                     QDVRHDSAVWFSDDADEFAKKAAEVGILSMEDPDARSLSMLLIYGLKGIAAYAEHAAV
                     LGFEDEKIDRFLMEALASTTRDLSVEDRVALVMRAGECAVDVMALLDKANTTTYGNPE
                     MSRVPIGVRGNPGILISGHDLKDLEELLKQTEGTGVDVYTHGEMLPANSYPAFKTYAH
                     FAGNYGNAWWRQAEEFASFNGPILMTTNCIIPVKEAYRDRIFTTGVVGYPGLKHIPDR
                     PRGGMKDFSEIIALAKTCAPPTEIETGELVTGFGHHQVLALADRVVDAVKSGAIKRFI
                     VMAGCDGRHKTRGYYTDVASALPPDTVILTAGCAKYRYNKLALGDIGGIPRVLDAGQC
                     NDCYSLAVIALKLKEAFGLNDINELPISFDIAWYEQKAVAVLLALLFLGVKGIRLGPT
                     LPAFVSPNVLKVLVENFNIRPITDVGTDVEAMMQGR"
     gene            14912..15601
                     /locus_tag="PLW16_05945"
     CDS             14912..15601
                     /locus_tag="PLW16_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB70480.1"
                     /translation="MRNIRDILTKSQLFGGLPEEHLAEVEKIAVERRYEKGEVIFADG
                     DEGIGFYLIASGSVNVYKLSPDGKEQILHIVKEGETLGAVPVFSGKSFPANARAITRS
                     HLLFFDRQRFVRMITGKPSLTMNLLALLAMRLREFTLQVENLSLREIPGRLAAHLLHL
                     SREQGDRDVVALNLSKARLANLLGTGPESLSRALGALKRKGLVEEDGPRLRLLDRDRL
                     EALAEMGKDAG"
     gene            16053..16295
                     /locus_tag="PLW16_05950"
     CDS             16053..16295
                     /locus_tag="PLW16_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HPB70481.1"
                     /translation="MNAYDMIETKPEVMRGKPVIKGTRIPVDLIVRKLGEGASFEDLL
                     DGYPRLSREAIQAALIYAADMIRNETTIFLKTGTVD"
     gene            16316..16681
                     /locus_tag="PLW16_05955"
     CDS             16316..16681
                     /locus_tag="PLW16_05955"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HPB70482.1"
                     /translation="MADESCDFSIIRALRSVGYSVDAIAEISPSLPDEAVLKLAVAEG
                     RLLITEDKDFGEWVFAHQHAMAGVLLIRYPASMRSSMVATVIDLVGVHSPELDGRFTV
                     LEPGRARIREKSKSNIHTD"
     gene            complement(16798..17394)
                     /locus_tag="PLW16_05960"
     CDS             complement(16798..17394)
                     /locus_tag="PLW16_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB70483.1"
                     /translation="MTTKTMAPIALYDWYEKGLYACRSKNFIYAENAFNKAIESNPRD
                     ANAYYWRAFVHKRTNSYEKAIEDYNRAIEISPNTSRFYTKRANAYCKLEQYQPAIEDY
                     TKVIRMEPGNAEARFDRAVTYMQTGEYRHAVDDYDRFISLKPNYADAYAYRGISYYYL
                     GKYSLCYRDALRASKLGNNELLEIFKENMALLRSSRTI"
     gene            complement(17615..18226)
                     /locus_tag="PLW16_05965"
     CDS             complement(17615..18226)
                     /locus_tag="PLW16_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70484.1"
                     /translation="MLKLINPQADINPYVWLLGELPNRNVAQDQEFQRIYRLYWQLNP
                     ARLSKEYLAAYFGHLEQLKGHPEHITVEAVARHLLPIPTHGNGRQSLQFSFASKLVHM
                     LNPEQPVYDSMVESFFFLPSGGANETTEQKIARLLASYEFLHTEYGRVLEQGLLAQAI
                     TQFRARFSVNAAYTDRKIIDTLIWKFVGFLKRGAVRKGIVVYG"
     gene            complement(18306..18971)
                     /locus_tag="PLW16_05970"
     CDS             complement(18306..18971)
                     /locus_tag="PLW16_05970"
                     /inference="COORDINATES: protein motif:HMM:NF021064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HPB70485.1"
                     /translation="MAKQPKRIPPFNPLDKTNLGESVAEAMLQQKVHLLPPDKPFIGA
                     GIYALYYEGDFPLYADIAEQNRADQYRWPIYVGKAVPAGARKGGYGLDADPGQALYRR
                     LAEHAGSINDAKNLNLADFRCRFLVVDDIWIPLAESLLVEMYSPLWNQKIDGFGNHDP
                     GKGRYNQQRSPWDVIHPGRAWAYKLPCPSNDERIVREGAAAYITRTKIKIMARQADAR
                     DEE"
     gene            complement(18980..20155)
                     /locus_tag="PLW16_05975"
     CDS             complement(18980..20155)
                     /locus_tag="PLW16_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HPB70486.1"
                     /translation="MNNGRSSIELFTGAGGLALGLEQSGWHHSALIEWDEHACSTIHL
                     NESLGHPLTKGWRLFPDDVRTISYLDLSDHVDMVAGGPPCQPFSLGGKHRAYQDKRDM
                     FPEAVRAVRELRPKCFVFENVKGLLRQTFSSYFNYIILQLSYPMIIRKGDEDWQSHLS
                     KLERHHTGAKEADLSYRVVYRLLDAAEYGVSQHRHRVFIVGFRSDLQKEWSFPEPTHS
                     LDRLLWDQWVSGEYWEEHRVPKSKLPAKHAKLAARIRRLESDFSLFPPPGERCKTVRD
                     ALRGLPDPRNKSDTVPNHEFRDGARPYHGHTGSSLDLPSKALKAGDHGVPGGENMMAL
                     PDGSYRYYTVRESARLQTFPDDYVFSGSWTEAMRQIGNAVPVELAAAVGKSILKQVT"
     gene            complement(20152..20592)
                     /gene="vsr"
                     /locus_tag="PLW16_05980"
     CDS             complement(20152..20592)
                     /gene="vsr"
                     /locus_tag="PLW16_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HPB70487.1"
                     /translation="MEQREKQKMMRYDPLTDAERSERMSRIRNADTKPEMIVRRLVHG
                     MGYRYRLHARDLPGNPDLAFRPRKKVIFVHGCFWHQHGCRQYRQPRTKREFWEPKLAK
                     NKERDAEVRRQFRKLGWRVMVVWECQVKKETVVRNRVKRFLDEK"
     gene            complement(20739..21407)
                     /locus_tag="PLW16_05985"
     CDS             complement(20739..21407)
                     /locus_tag="PLW16_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70488.1"
                     /translation="MAPVTVFQRIAIAPPRAAIYRRLGFRSASTRLSEAQRRETEAAI
                     DAAADLIRLAGASRRLAVVERGPDRIVLDGGAVLASRRLADFLGTSREVLLMGATAGE
                     AIMAAIRADMAEGRATRGVVLDATASEMTDAALDWITAVENRALRREGRTLLTARYSA
                     GYGDLDLEHQTLFHRLLELNRLGVSLTPSRILLPEKSVTAITGLREIAVETPRDAATR
                     NEAI"
     gene            complement(21419..23011)
                     /locus_tag="PLW16_05990"
     CDS             complement(21419..23011)
                     /locus_tag="PLW16_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70489.1"
                     /translation="MSGPDRNNPYNFDAFLAWRQAVDYYAADAFIQKVVRRFTGDEAD
                     RVDAAARAVSRKASYRWRDLAERIALPENRPFMMHYDGHHRRIDRICRPGETETLERE
                     VFAEAFYSEKTSPWEKLIKMYLIYQNGEACVACPITCTEGLVMILEKFADTPETKAIL
                     AHCKEGDGDGFAIGAQYLSEIQGGSDVPANLLEAVEEDGVWRLYGTKFFCSATHADYA
                     VVTAKPKGSEKVGLFVIPSWLPGDKAKEIRNGYTIDRIKWKIGTSELTTGELTFNGAV
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAFMTRAVREAKQYAEFRDAFGVTIGRF
                     PMVAGQLAAMDHAAKRTTAGAFKLYRDFLTLEGGLKGGLDTGETETAWRKRFVIRELI
                     MLQKLAASWDCTDVIRAAMSVFGGHGVMEDFSSLPRLYRDSAVNELWEGPRNVLLTQI
                     FRDLQRVKDRYALAAFVRDLLDGLEEAVVAPLAAEAEALLAHAHLFGMDAATLDVCRR
                     WDAFCHTLTHAYQDLAFKDVEG"
BASE COUNT         4247 a         7111 c         7032 g         4678 t
ORIGIN      
        1 cttcatttgg gcctctgctc cgtccaggat cttcctggcc gcgtccaggt ccgcggcggc
       61 gtacgtttcc gcgccggcgg ccaccgcggc gttcatcgcg gcatcggccg cggacttctc
      121 cgcttcaggg ggttttgcac aacccgccaa cgccagagcc aatacgagca caacgaccaa
      181 cgatgaaaaa tatttgaaat gattcatgcc agtcctcctc cttttgggat cctcggcgtc
      241 ctccggccga aaacttccgt aaaaatatgc ttttcctcga gtttgtcaat gcattttttt
      301 gatcgaatgc cccggttgcc tccgatgggg gacgttgcgc ctgcctccct aggaaatggc
      361 gtatccgccg tccagaaaga gggtctggcc cgtcatgtaa tccgacgccc ccgaggcgag
      421 gtagacggcc agccccacga tctcgttcgg cctgccgaaa cgccggaacg ggatctttcc
      481 gaggagaaac gtttcgtttt ccccctcctg ctggtctttc gtcatctccg tcaggaacca
      541 cccgggaacg atgcagttga cctggatgtt gaagcgcgcc cactcgaggg ccagggtctt
      601 cgtcatgtgg tgcaccgcgg ccttcgaggc cccgtagtgc aggctgttgg gaagcgcccc
      661 gtgtccgaga atcgagccga tgttgatgat cttgccttgc cgctgccgga tcatcaccct
      721 gccgaccgcc cggcacgcga gaaaaacgga ccgcgcgttg gtgttcatca ggtgctccca
      781 gtccgcgacg gtcaggtcca ccgatcgctt cgagccgccg atcccggcat tgttgaacag
      841 gacgtcgatc cgtccgtagg ccttggcgac ctcggccgtc atggattcga cctcttcctc
      901 gcgggtcatg tcggccggca cggcgatggc ttcgccccct tgtccgacaa tctccgccgc
      961 ggtctgttcg atcaggctcc gggtccggct cacgacggcc acccgggcgc cggcttccgc
     1021 caggccaagc gccatctccc ggcccagtcc cttgcttccg ccggtcacga tgactactcg
     1081 ctgcgccagg ttgaattgtt ccatcacacc catgtctttg cctcctcttt ccacgtgatc
     1141 ctcgctgcat cgccgggccg ccggtcatgg aggcccggcg ccggggcggg aacacggtta
     1201 tcaacgacga acacgctata ccatgaaacg gccaaggccg cttgtcgaat tccgccttga
     1261 tcccgtcagg ggtctaggtt ttaccggttg caccgaccgt gggtggcgac agggcgccgc
     1321 cggccgccgg catcatcgcc gctggacgat ttcggcggaa gctgataagc tcaccaaagc
     1381 ggttacgacc gcaccctttc cataacggcc cagacccgga ccgccggggg cgccaaaaac
     1441 ggtatccatg cgaggacccg agaccatgaa gttcaacccc gccgtcgtca gctatttcct
     1501 gcagcgagaa agcaccaagc ggaacatccg gcagcttttg aaattcctct ttgccttggc
     1561 cgtcctggtg tctgtctaca gcgtgctctt ccattttctc atggcccgcg agggacagaa
     1621 ccattcgtgg atcacggggt tctactggac cctcacggtg atgtccaccc tcggcttcgg
     1681 ggacatcacc ttccattcgg acctgggacg cctcttttcc atggtggtgc tcgtaaccgg
     1741 gatgatcttt ctcctgacgc tgctgccgtt cacgttcatc aagttcttct acgccccctg
     1801 gatcgaggcg gaagcgagga agcgcgcgcc gcgggaattg cccccggaca cgcgggggca
     1861 cgtgatcatc accagctaca acccggtgac catggccctg atcgagaaac tcacggacca
     1921 tcggatcgac tacgtcctca tcgtgagcga cttcaagcat gccctggacc tgtacgacat
     1981 cggcgtccgg gtcgcggtgg gaaacgtcga cgaccccgac acctatcggc ggatccgcgt
     2041 cgagaaggcc gcgctcgtcg tcgccgccga ccgcgacgaa atcaacacga acatcgcctt
     2101 caccgtccgg gagctgaacg agcgcgtgcc catcatcgcc acggcagact ccccctattc
     2161 ggacgacatc ctccgcatgg cgggcagctc caaggtcctg cagatctacg atattctcgg
     2221 gcgttccctg gcggcctgga ccgtgggtgg cgactgccgg gcgaatgtcc tcggccgctt
     2281 cgacacgctt ctcatcgccg aatttcccgc gaccggcacc cccctggtcg gcaagaccct
     2341 ggttgagagc ggcctgcgcg agaacttcgg ggtgaacgtc gtggggatct gggagcgcgg
     2401 acgcttcgag attccccgcg ccgacagccg gatcgagcga accagcgtgc tggtcctggc
     2461 cggctccgag gagaacctgg cggcctatga cgaggtgtac gccttttacc acatctgcaa
     2521 gctcaccgcg gatccggtgc tcatcgtcgg gagcgggcgg gtgggcgaca agattgcgga
     2581 gcgattccag gagcggggaa gcccctacct ggtcatcgaa aagaacccca aacggctgcg
     2641 cgacgagcgg cactatgtcg tcggggatgc cgccgacatc cggaccctcg agcgggcgtg
     2701 gatcgacaag gcacccgcgg ccgtgatcac cacccacgac gacgacacga acatctatct
     2761 caccaactac ctccgggcgc tgcgcccgga catgcagatc ctgagccggg ccaatctcga
     2821 ccggaacgtc tcgacgctgc accgcgccgg cgccgatttc gtcatgtcct atgcctctct
     2881 cggcgccaac gcgattttca actacctgaa aaacgaagac acgtggatgc tggccgaggg
     2941 gctcaacatc ttccgcatgc gggccccgga cgcgcttgtg ggacggaacc tcgcccattc
     3001 cgggatccgc aatctgacgg actgctccgt cgtcgccatc cggaacgccg acggcctgtc
     3061 gatcaatccc gatccgctca cgccgatcgc ggcggatgcc gagctcatcc tgatcggcac
     3121 caacgagggg gagcgcaagt tcctgcagac gttcacgccc gcttgacgcc gccctggcgt
     3181 tccttcggcc cgatcctcag agcggatcgg gccgaaggaa cataaaatgg atcaagtccc
     3241 gaattcctgc ttcatctccg tgtagaggtc gagcttcttg acctttccca ggggcgtgag
     3301 gggcagtatc ggccggatga tgacgtcctc cgggagcttg aagtgggaga gcttgtcctt
     3361 gaggaattcc ttgatccctt ccggcgtggg actcgtcccg ggcttgggca tgatgtaggc
     3421 gcgcccgacc tcgccccact tctcgtgggg acggccgacg accgccgcca gcatcacgtc
     3481 cgggtggcgg cagatcgcct cctcgatttc gggcggatag acgttctcgc cgccgcggat
     3541 gtacatctcc ttctttcggc caacgatcac gagataaccg tcgtcctcga agcggcccat
     3601 gtcgccggtg tggaggaagc cgtcagcgtc gaagaccttg gcgtcctctt cggggcgctt
     3661 ccagtagccc ttcatgacga ccgggccctt cacgcagatt tcacccgcct cgcccgtggg
     3721 gacgatcctc ccgtcttcgt ccttgagggc gagatcgacc tccgggatgg gccggcccac
     3781 ggagtagctc agcttggcct caccgtcgtc gggatgcgta taggtgacgg cgccgctcac
     3841 ctccgtcagg ccgtaggcgt ttcggggggt gatgccgagc ttctgccgga aggccaggat
     3901 cagctcggac gggcagggct gggccgagac gacggcgatc ttcatggaac tcaggtcgta
     3961 cttgccggcg ttggggtggt tcagctccat ggcgtacatg acggggacct gtcccagggc
     4021 cgtgcatcgc tccgcggcga tgagcttcag cgtttcctcc ggatcgaaca ggtccatcag
     4081 gacgatcttg ttgccggcat tgacggcgga catgaccccc atcaccgcgc cgccggcatg
     4141 gctgaacggg aggttgagga ggagggagtc ccccttgacg acgccggtgg tcctcaactg
     4201 ggcgtccgtc atggcgatga cgtttcgatg ggtcagaatg gcggccttgg gaacgcccgt
     4261 ggtgccggac gtatagatga agatggccgg atcgtcctcg cggacggcgg cctccgcttc
     4321 cttcagggcc gccgagtcgg ggtcggcgag gaaggtctcg tacaggagca ccccctcctt
     4381 ctcgctgtcg agactgacga cgtggcggag cttggggagg tctttccgga cggcgtccag
     4441 attgcccata aaatccgccc ccatgaaccc gttcgcgaga aacaggacgg aaatctcggc
     4501 gtcgttgata aagaatttca gctccggcgc cgtcgttcgg atgctcatgg gaatgccgac
     4561 ggccccgatc ttggccgcgc cgaaatagat gaacagcgtc tccggccaga gcgggaggta
     4621 gatcccgacc cggtcgccgc gccggatgcc gagcttgagc aagcccatcg ccatccggtc
     4681 cgtttcgcgg tcgtaggcgc cgtagtcgat ccggcgatcc ttgaaaacga tcgcttcatc
     4741 cttcggtatg gttttgccgt gcttttcgaa ataatccccc aggaccatgt ggttccccct
     4801 ttcatcatcc ggttgatccg cgcattttcc tcctccccgg gggaagcgga caaccaccgg
     4861 gtagaaacgg cgcggcgccg gatggacgat tccacgccgc gcatcggcgc acgccggccg
     4921 tcgcccgaaa aaagcgggcg atcggtttag ctgagaaata aagaggtgtc ttgaggccgg
     4981 agaggccgga ggccacaggg cctccggcct tctgttgcat gcaaaactgg gtaaagctat
     5041 tttccgtgtt tggacaggat cgcgtccacg cagatcacgg cggaactggc cgccgtgttc
     5101 atcccgacgc cccggcccag gtagcagtcg ccggagaagt acagaccggg cacctccgag
     5161 agctccacgg gcagccggta gcgaccggtc atgcccggcg agcgggagag accgtcgatg
     5221 ccgatcgtga acccgtcctg tttccacacc agattgctct ccatgttggg gaacatccgg
     5281 ttgaggacga cccagaactg atccagggtc ttcttgcgga aggccgcgtc tcccacctgc
     5341 gccggcgtgc cgcaggcgcc catgaaggcg atcatgtggc cgggaggcgc gatcgtgtcg
     5401 tcgaagttcg tatgccccag gatctgcagg ggcatgccca gtccggggac gcgccaggcc
     5461 gacaggtagc atttgtcgtc gaacaggggc accttggtcc cgatggtgta tcccatggag
     5521 gcgctggtct cgaactcgca ttcgcgcatg cgcttcgccc acgccggcgc cagttggtcc
     5581 atggccgcca tgtcgaggaa ccgcagcacg ttccagatcg gcgcggcgat gacgacgatc
     5641 ggcgcggaat agttgccgcc gtccgtcacg agctccgtcg gcccgctttt cccgaccaag
     5701 accttgcgaa ccggcgtgcc gagcttaagc gttccgcccc gtttcttgta ggcctccgcc
     5761 atggcgttca tcatggtctg gacgccgccc aggggcatcc gcaccgcgtc gaacggaagg
     5821 tcggggctct tcgagacgcg catctggtcg cggtagatgt ggatgaactc gcccgcggag
     5881 atgatgtccg attccgtcag ggtcgtctgc accatgccga cggtcttgca gaattcgccg
     5941 atcttgggcg atttgacccg ctcctcgagc agtttcgtca gggaaacgtt gtcgtaatgg
     6001 tcgatcatct cgtcggaggc gttcttgatc caggcctgca aatcgctgag ctctttccgc
     6061 tccgcctcgc ccaggttgaa agactccatc atcggcttcc atttcccgtc ctcgccgtag
     6121 aacatgaacc cctcgaggtt gtgcgaccag gggatgtccg ttccgagctc cgtgagcagc
     6181 ttggcacagg acgaatgcac gccctgatcg acgcagtggg tgcccatgtc gacccgccag
     6241 ccgccgggga gttcgaaggt tcgcgaacgc ccgccgacga ccttgctctt ttcgaagacc
     6301 gccgtcttga aacccttgtt ctgaagtata ccgccgacgc cgagtccggc aatgccggca
     6361 ccgataatga tcacatcgta tttttccatt acatcctccg tcctactttt tgccgagttc
     6421 ctgcttcatt tccgtgtaga ggtcgagctt cttgaccttt cccaggggcg tgaggggcag
     6481 tatgggccgg atgatgacgt cctccgggat cttgaagttg gacagcttgt ccttgaggaa
     6541 ttccttgatc tcctcgggcg tggggttttt ccccggcttg ggcatgatgt aggcgcgccc
     6601 gacctcgccc cacttttcgt gggggcggcc gatgaccgcc gcgagcatca cgtccgggtg
     6661 cttgcagatc gcgtcctcga tctgcggggg atagacgttc tcgccgccgc ggatgtacat
     6721 ctccttcttc cggccgacga tgacgagcga gccgtcctct tcgaagcggc ccatgtcgcc
     6781 ggtgtggagg aagccgtcgg cgtcgaagac cttggcgtcc tcttcggggc gcttccagta
     6841 gcccttcatg acgaccgggc ccttcacgca gatctcgccc gcctcgccct ggggcatgat
     6901 gttgccgtcg tcatccttga gcaccatctg gatcgaggga accggcttgc cgacggaata
     6961 ggtcagcttc tcctcgccgt ggctgggatg ggtgtaggtg atggcggcgg tggtttccgt
     7021 caggccgtaa gcgttgagtg ggatcacgcc gacgatcttc ttgaaggccg agatcagctc
     7081 cgaggggcac ggctgggagg agacgatggg gagcttgagg gtgctgatgt cgtacttgct
     7141 caggttggga tgggaaagct ccatggcgta catggcgggt acctgcccca gcgtcgttgc
     7201 cttctcttcg gaaaccaggc ggagggtttc ctcgggattg aaggcgtcca tcatgacgac
     7261 cttgttgccc acgttcacgt tgcagatgat ccccatcacg gcgccgccga catggctgaa
     7321 ggggaggttc aggacgatga tatgcccgcg atggtatccg gtggcctcga tctgggcgtt
     7381 cgtcagggcg atcaaattct tgtgcgtcaa ctgggcggcc ttgggaacgc ccgtcgtgcc
     7441 agatgtgtag atgaagaggg cggaatcgtc ctcctggacc gcatccttgg ccttgttcag
     7501 caccgccggg tcggggtcgg tcaggaactt ctcccagagg aggacgcctt ctttttccag
     7561 atcgaagcat acgacgtgtt tcagcgtcgg catctcctcc cggacggcgt ccagattcgc
     7621 catgaaatcc ccgcccataa aggtgctggg catgaacagg acggagctct ccgagtcgtt
     7681 gacgaagaat ttaagctccg gcgccgtggt ccgggcgctc atgggaacgc cgatggcccc
     7741 gatcttggcc gcgccgaagt aggcgaagac gacctcgggc catagcggcc cgtagatcgc
     7801 gatccgatcg cctcgctgga cgcccagctt caggagaccc atcgccaccc ggtccgtcgc
     7861 ctcgtcgtac tgcttgaacg tgatccgtcg ctccttgaag acgagcgcct cgttattggg
     7921 gatttcctgg ctgtgcttct tgaaaaaatc tcctaaaacc atgcccaact ctcctttcgt
     7981 ttgtatgctc cattcatcgc ccgtgcaatg aacgtgtttg cggaagaatc tccggttcag
     8041 ttccggcctt gattgagtac cgttcaccta atttaaggga aacataatgt caagagaaaa
     8101 aaggcctgtg aataatccgt cgggccctgg aaagtgcgga ggttctgatt gtattgctat
     8161 tacagtatat tagactgtca gacccgggga gggcccgtcg aacggccgat tcgggcccac
     8221 ccggggtaga gaccggttcg aggggtgaat ggggttcata aggaacgcct tgccggtcgg
     8281 aaccggaggc gggtggaatc agaactcgat tcgcaccaga tattccacgt tggggggttc
     8341 ccggagggcg gccgtcatca tcagcgtcat caggtgacgg acctccgtgt acttgtcgaa
     8401 ttccttgtgg tcgatgatga tccagcggag ggcgaggtgg gtgtaggacc ccagaaacat
     8461 gtaccagaag acacgcggat tcacgtccgc gcgaaacgtg ccttcctgtt tgccctcctc
     8521 gatgacctgg cccatcatcc gggcgaattc gttctgtttc ggctggatcg cggtctcgta
     8581 gtaccggcgg ttgaacaggg tatacatgaa gtagaccgtc atgaagtccc gaatggacag
     8641 gaagctcagg ccgaaggaac gcatcagccg ccataatttt tccggcgggg aggggccggc
     8701 ctgcgggttg aagatgttgg aaaccctttc cagataggcg ttcaggttcc tgtcggacag
     8761 ggcgaacagc aggtcttcct tgctcgaaaa gtactcgtaa atcgtgcctt cggccacctg
     8821 ggcctcctcg gcgatggccg cgatcttggc gtcggcgtag ccctgccggg cgaaggtctt
     8881 ctccgccgcg agcaggatcg cctgctgccg gttcgtcacg gcgggcttgc ccttcgttgt
     8941 cagcatgggg aggagcaggg ccatgatgtc gtcgagatcc gtgcagctct tctcgatttc
     9001 gccagtgacg atgctgctga gggcttcgat gtcgatcgtt ccgaagatga tgtgcttcat
     9061 cagtttcatg tcgatgtcgc tccggaacac gccctcccgg gcgccctcct cgaggatgtt
     9121 gaggaggatg gcctcgtatt ccttcatcag gcggtacgct tcggaccggt aaaaatcctt
     9181 gttggaccgg caccggaaga ggagattcgt cacgtgattg ggatgcgaat cgttgtattt
     9241 cagcatgtgc cagatcatct tgctcaaccg ggatttcggt tcgatgatgc cctggagatg
     9301 ctcgcgcaaa agggcggttt cctcgcgggt tcgctcggcg accacggaaa agatgatgtc
     9361 attcttgccc ttgaagtatt tgtaggcgag ggaatcagcc acctgggccc gttccgcgat
     9421 cttcgcaatc gtcacctcct cgatgctgct ctccaggatg agggcctcgg cggcatcgag
     9481 gatgcgtgaa cgggtctgct gttttttctt ctccatgcct gagcgtcccg aaaaatttaa
     9541 agggtgagat ccgcgcgacc tttggggagt cacataacgg gctgggcgga ccgcacctct
     9601 ccgacgaggg ccgtcgccac gcgaacggcc tccacgccat cccgggcata ggaagcgccg
     9661 atcgaggcgg cgaacccggc cgtcacgacg gcgcccccca ccataaactt cgccgccaag
     9721 ccggcgtccg cggcccgggc gatgacatcc ttcattcgga ccatggtcgt tgtcatcagg
     9781 gccgacagcc cgatcacctc cggccggtgc tcctgcgcgg cggcgacgat cgcctccgcc
     9841 gtcacgtcct tgccgagatc cacgatgtcg aagccgtggt tccgcagcat caggacgacg
     9901 atattcttcc cgatgtcgtg gatgtccccc gcgacggtgg ccatcaggac cgtcccccga
     9961 tgcgcccggg cggttccttc ccgtctgagg aggggttcga ggcgcgcgag cgccgccttc
    10021 atggcctcgg ccgcggcgat gagctggggg aggaaatagg tcttcttgtc ataccggtcg
    10081 ccgacctcga cgatcgccgg gatcaggcag tcgtcgacca gccggtcggc ggggatcccg
    10141 gctgccaagg ccgcgtcgac cctcgcaacg atctcctccc ggttgccatc gagaatggcc
    10201 tcccgcaccc gttcctccgg agagcccggg tcggccgccg gtggaacagg ctccacgctc
    10261 ggcgcggttt gggtcgcgtg ggcgatgtag gccgccgcgt cccggtcccg gcccagcagg
    10321 acgtcgcccg cccgccgcgc atcggccagc acgtcgctcg acgggttggc gatggccatc
    10381 gtcaggccgg ccgcctgggc catggtgaga aaggcgctgt tcagccggtc ccgctcgggc
    10441 atcccgaagg agacgttcga caggccgagc accgttcggg cgccgaaccg ctccgcgctc
    10501 caggcgacgg tcttcagggt ctcggcggcg gcccgggggt tggaggcgac ggtcatgacg
    10561 aggccgtcca cgacgatgtc ctctttggtg aatccgtggc gccgcgccgc ccggtagacg
    10621 tcgcgcacga tttgtatgcg tttttccgcc gtggcgggga cctcgccgcc ggcgagcggc
    10681 aggaggatga acatcgcccc gtagcgggcg gcgagcggca ggagttcggc catcttctcc
    10741 gactcgccgg agatggagtt gatcagggcg cggccggggt agagccggag cgcggcggcg
    10801 agcgtgtcga cccgggcgga gtcgaggcac agcgggagcg gcgtcgcggc ggcgaggagc
    10861 cggacgaccg cgcggatggt ttcgacctcg tcgatgtcgg gaacgcccac gttcacgtcg
    10921 aggaggtccg cgccgcgctc ctcctgctcc cgggccatcc ggcggacgag ggccagtttg
    10981 ccctcgcgca gctcctgctg gagcgccttc ttgccggtgg ggttcaggcg ttccccgatg
    11041 acgacgaggg gatcgccggg ggcgaagatc cggtgggcgc gggcggagct gatggcggcg
    11101 agcgacctgc gccggggcgg ctgcggcgct acgccgtcga gggtggtgcg cagggcggcg
    11161 atgtgggccg cggtggttcc acagcagccg ccgatgaggt tcgccccggc ggcggcgagt
    11221 tcccgcgcgg ccgcgccgaa ggcctccggt gccatgtcga aaagggtctt atctccgacg
    11281 agccgcggca tcccggcatt cggtttggcc ataagcggga cggtcgcgta gggcttcatg
    11341 gcggcgatca gggggaccat cgcttccggc cccgtcgagc agttgcaccc cacggcgtcc
    11401 gcgcccaggg cctgcagggt gatcaaggcg gtcaccgggt ccgtgccgtt cagcgtccgg
    11461 ccgtccgcct cgtacgtcat ggtgaccatg accggcaggc ggcagatctc cttcaccgcg
    11521 atgagcgccg cccgggcctc ctggatgtcg agcatcgtct cgatgacgaa gaggtcgacg
    11581 ccgccttcca gaagccccgc cgcctgttcc ctgaagatgg ccaccgcctc gtcgaagggc
    11641 aggtcgccga agggctccac gaagcggccc gtcgcgccga tgtccccggc gacgagcgcc
    11701 cgccttcccg ccgcccgccg ggcgaggcgg gcgaggtccc ggttgatcgc gtggacgtcg
    11761 gtgtgcccgt acggggcgag cttcggacgg ctgccgccga aggtgcacgt gtagaggatc
    11821 gccgcgccgg cgtcgagata ggcgcggtga atcccgctca gggtgtcggg gtgcgcgagg
    11881 caccacgcct ccggagagac ccccgcgggc atgccgacac gctggagttc cgttcccgtg
    11941 cccccgtcga ggatggcgag gccgtcccgc cagaggccgc gaaaggtctt gcgcctagac
    12001 atggcgacct cccggggggc gccgcgccgc ggccgcaatg ggtatttcat tgaaaacacg
    12061 gttatattcc acggatggag gattcctttc ccgacagcgt ctcataaccg gatcgaccgg
    12121 ccttgtcaat cctcatcttc cgcaccggtc cggtcgccgg tgtcgcagac aggcaaaagc
    12181 cccataaccc ccgtgggtta tggggctcgc gaatttgaat gtggccgggt tcggcggcgc
    12241 gttgccgacc gatccaccgt ttcgatgatt gcccagtgcg gcggagccac cgcccttcca
    12301 tcatcgtgcg aggcgtcttc aggagcgctt tcccggtctc gattgactcg gggggttcct
    12361 tcccggcgac gggcgcccgc ggaccgcccc gccgcacgac ggtctaccgg cgcgccaaaa
    12421 cctttcccct gacccccagc acgacctcgt tcagggggat cgtcacgccc gcccgttcca
    12481 tccccttccg gacgatcgcg ggcagccccg cgcagcaggg cacctccatg acggccgtgg
    12541 tgacgctcct gacgccggcg gtcttgaaaa tttcggtgaa cttttccacg tacgcatcga
    12601 cgtcgtcgaa cttcggacag cccatcatca ccaccttccc cttcagccag tcccggtgca
    12661 gggttggaaa ggcgacggcc gtgcagtccg ccaagaccag caggtcagcc cctttcagga
    12721 agggggcgtt cgcgggaatc agccgaatct ggatcggcca gtgcgtcagc gccgactcct
    12781 cctccggccc ccgcccgatc tgcgcgggcc ggttggccgc ctcgcaggcg gcgggcgatg
    12841 cgaagagctg gacgtgcgtc gacgggcatc cacacggcag cacggccttt tcccgctccg
    12901 cgaggtggcg ctgggcggcc gcctcgtcaa aaggaggcgc ctcgcgctcg acgatgcgga
    12961 gcgccccggt ggggcactcc ccgatgcaag cgcccaggcc gtcgcagaac gtctccgaaa
    13021 ccacccgggc ctttccgtcc acgatttgca gggcgccctc ggcgcacccc gtcacgcatt
    13081 gaccgcaccc gtcgcagagc gcctcgtcga tctcgatgat cttgcgcaag atcctgttca
    13141 tgacacgacc tccagcggaa atcaccgccc ctgcatcatc gcctcgacat ccgtccccac
    13201 gtccgtgatc ggccggatgt tgaaattttc caccaggacc ttgaggacgt tcggcgatac
    13261 gaacgccggc agtgtcggcc cgaggcggat gcccttcacg ccgaggaaaa gcagcgcgag
    13321 tagcaccgcc accgccttct gctcgtacca ggcgatgtcg aaggagatcg gcagttcgtt
    13381 gatgtcgttc agcccgaagg cctccttcaa tttcagcgcg atcacggcca gcgagtagca
    13441 atcgttgcac tgcccggcgt ccagcacccg gggaatgccg ccgatgtcgc ccagggccag
    13501 cttgttgtag cggtacttgg cacagcccgc cgtcaggatg accgtgtccg ggggaagcgc
    13561 gctggcgacg tcggtgtagt agccgcgtgt cttgtggcgg ccgtcgcagc cggccatgac
    13621 gatgaagcgc ttgatggcgc ccgacttcac cgcgtccacc acccggtcgg ccagggccag
    13681 cacctgatga tggccgaagc ccgtcaccag ctcgcccgtc tcgatctccg tcggcggcgc
    13741 gcacgtcttg gccagcgcga tgatttccga gaaatccttc atcccgccgc ggggccggtc
    13801 ggggatgtgt ttcagcccgg ggtagcccac gacgcccgtg gtgaagatcc ggtcccggta
    13861 ggcctccttg accgggatga tgcagttggt cgtcatcagg atcgggccgt tgaaggacgc
    13921 gaattcctcc gcctgccgcc accaggcgtt cccgtaattg cccgcgaagt gggcgtaggt
    13981 cttgaacgcg ggatagctgt tcgccggcag catctcgccg tgtgtgtaga cgtccacgcc
    14041 ggtcccctcc gtctgcttga ggagttcctc caggtccttc aggtcgtggc cggaaatcag
    14101 gatgcccggg ttgccccgga cgccgattgg aaccctggac atttcggggt tcccgtaggt
    14161 ggtcgtgttc gccttgtcca gaagcgccat gacgtcgacg gcgcattccc cggcgcgcat
    14221 gaccagggcg accctgtcct cgacgctaag atcgcgggtc gtcgacgcca gcgcctccat
    14281 gaggaagcga tcaatttttt cgtcctcgaa gcccagcacg gcggcgtgct ccgcgtaggc
    14341 ggcgatgccc ttgaggccgt agatcagaag catgctgagc gaacgggcgt ccggatcctc
    14401 catggagagg atgccgacct cggcggcctt cttcgcgaat tcgtcggcgt cgtcggaaaa
    14461 ccacacggcc gaatcgtgcc ggacgtcctg cgcatccgcc ccgcagattt ccttcaacgc
    14521 gtcccgcagg gccagcgcct cgcggatgag cgccgtgatc cggtcgttgt cgaagttcac
    14581 gttggtaatc gtcgcgaata gcgcctcagc gacaaagcgc ccgtagcggc ggtccagcgg
    14641 acccttctgt ccggcctttt cggcatagag ggcgatcccc ttcaggacat ggatcagcaa
    14701 atcctgcagg ttcgcggttt cctccgtctt cccgcacacc ccccggacgc cgcagccttc
    14761 attcttcacg gtttcttgac actgaaagca aaacatgtga aacctccttt ctccgattcg
    14821 atgtcgttgc gttcctgtaa ccgaaactga cacgcgcgtc cttgacttat gtcaagaagc
    14881 ggatatagtt ccgcctcaaa gggcttcatc catgcgaaac atacgcgaca tcctgacgaa
    14941 gagccagctg ttcgggggcc tgcccgagga gcacctcgct gaggtcgaga agatcgcggt
    15001 cgagaggcgt tacgagaagg gggaggttat cttcgccgac ggggacgagg gtatcggatt
    15061 ttacctcatc gcctcgggaa gcgtcaacgt gtacaagctc tctcccgacg gaaaagagca
    15121 gatcctccat atcgtgaagg agggcgagac cttaggcgcc gttcccgtct tttccggaaa
    15181 atcgttcccc gccaacgcgc gggcgatcac ccggagccac ctgctgttct tcgaccggca
    15241 acggttcgtc cggatgatca ccggcaaacc gtccctgacg atgaacctgc tggcccttct
    15301 cgccatgcgt ctccgggagt tcaccctcca ggtcgagaac ctgtcgctcc gggaaatccc
    15361 ggggcgtttg gccgcccacc tgcttcacct ttcccgggag cagggggacc gggacgtcgt
    15421 tgcgctgaac ctctccaagg cgcggctcgc caatctcctg ggcacggggc cggaatccct
    15481 gtcccgcgca ctgggagccc tgaagcgcaa gggactggtg gaggaggacg gcccccgcct
    15541 ccggctgctc gaccgcgacc gtctggaagc gctcgccgaa atgggaaagg acgcggggta
    15601 agatcgctgg gcgcgatggg tgcgccccgc cgccgaggac gtcacccact gtccggccgg
    15661 cgggccggat ccgttgatct tgtatcctat ttaacccaat gaatcgtcag cggatagggt
    15721 tattatttgg atagaaagct ggacagcaac ggccaagacc atagaaaatc ctgtacgcta
    15781 aacaaccggg tgttcacgat gtcatggcac tcaacattta gccgataagc gatctccttt
    15841 ggggacagac aaaccatggc gtgcggatga aaaatatctg caaggccatc tgataaactc
    15901 cgccggctcg gaaaagtcct ctgttgtgga tggataagtg agaatgtgga gacgatgctg
    15961 ggaatcatca tgctcaggtt caagaagtaa agaaaaaatt gccctgaatc ctaacttctg
    16021 gtaccatggc aaccggcaag ggagggattt tcatgaatgc ctacgacatg atagagacaa
    16081 agccggaagt aatgcggggc aaacccgtca ttaaagggac gagaattccc gttgatctca
    16141 tcgtaagaaa attgggtgaa ggcgcttctt ttgaagactt gctcgatggc tatccaaggc
    16201 tgtcaagaga agccatccag gcggcgttga tttacgccgc cgatatgatc cgcaatgaaa
    16261 ccacgatctt cttgaagaca ggcactgtcg attgaatcca aaaccattga gatttttggc
    16321 agacgaaagc tgcgattttt caatcattcg cgccttgcgg tcggttggat attccgtcga
    16381 tgccatcgcg gaaatatccc catcattgcc ggatgaagcg gtattgaaat tggcggtggc
    16441 ggaaggccgg ctgttgataa cggaagacaa ggactttggc gagtgggttt ttgcacatca
    16501 gcatgcgatg gcaggcgtat tgctgatccg ctatccggca agcatgcgct cctcaatggt
    16561 tgcaaccgtg attgatctgg taggcgtaca ttcaccagag cttgacggcc gttttactgt
    16621 tttagaaccc ggaagagcaa gaatcagaga aaaatcaaag agtaacatac ataccgacta
    16681 aagccggcac gaacgttact ctataaaagt atgaattact gaggaggtta ttgatgaagc
    16741 agatagcccc ggcttcgaca gtggtaggca agcgatacgg ggattcttta ataaaattta
    16801 gatcgtacgg gaagatctta acagagccat attctctttg aatatctcca ataattcatt
    16861 gttccctaat ttacttgccc ttaaagcatc tcggtaacaa agggaatatt tccctagata
    16921 atagtaagat atgccgcggt aagcgtaagc atcagcataa tttggtttca ggctgataaa
    16981 cctgtcatag tcatcaacgg catgtcgata ttctcccgtc tgcatatatg taactgcccg
    17041 gtcaaagcga gcttcggcat tgccaggttc catgcgaatt acctttgtgt agtcttcaat
    17101 ggccggttga tattgctcca gcttacagta agcattggcg cgtttcgtat agaagcgaga
    17161 agtattcggg gatatttcaa ttgctctatt gtaatcctcg atagcttttt catagctgtt
    17221 tgttctttta tgcacaaaag cccgccagta gtaggcgttc gcatcccgtg ggtttgattc
    17281 aatggctttg ttgaatgcat tctcggcata aataaaattc tttgatctgc aggcatagag
    17341 ccctttttca taccagtcgt ataatgcaat tggtgccatt gtttttgtgg tcatgtttat
    17401 aatttatcat taattttaaa caactatcat tccatgattc taaaatatgg ttcatccggt
    17461 tgatgcgtac ttttgtctca ttcacttacg agtcaactta ttcgtgaaac ggagaaactg
    17521 aagataacca attgatagat ggtaacgtcc gccacaaccg gcgtgcaaaa gcagagcgaa
    17581 gcggcgcttt tgcacgtccg agttgatggc gttgttagcc atagaccaca ataccttttc
    17641 tgaccgctcc acgcttgagg aacccaacaa atttccaaat tagggtatcg atgatttttc
    17701 tgtcagtgta ggctgcgttt acactgaaac gcgcccgaaa ctgcgtgata gcttgagcaa
    17761 gaagcccttg ctctaacaca cgcccatact ctgtatgtag aaattcatat gaggctaaca
    17821 aacgagcaat tttctgttca gttgtttcat tggcgcctcc agaaggtaag aagaaaaaac
    17881 tttctaccat gctgtcgtaa actggttgtt ccggattcag catatggacg agcttgctcg
    17941 cgaatgaaaa ctgcagtgac tggcgaccat taccatgtgt tggaattgga agtaaatggc
    18001 gcgcgactgc ttctaccgtg atatgttcgg gatgaccttt cagttgctcc aaatggccaa
    18061 agtatgcagc caagtattct ttactgagcc gcgcagggtt cagttgccaa taaagacgat
    18121 agatacgttg aaactcttgg tcttgtgcaa catttcgatt tggcaattct cctaaaagcc
    18181 agacatacgg gtttatgtcg gcttggggat tgatcaactt cagcaacgct cccacattct
    18241 gattgatgat tgtgtacatg tgtttgtatg gctaacgaaa agctcacccg gagcgaagcg
    18301 gatcgctatt cctcgtcgcg tgcatccgct tgtcgggcca tgatcttaat ctttgtccgg
    18361 gtaatatatg ctgccgcacc ctcacgcacg atacgctcgt cattggaagg acacggaagc
    18421 ttgtaggccc atgcccggcc gggatggatg acatcccaag gtgaacgttg ctgattgtat
    18481 cgtcccttgc cgggatcgtg attaccgaac ccgtcgatct tctgattcca caatggagaa
    18541 tacatctcga cgagcaggga ctcggcgaga ggaatccata tgtcgtccac caccaggaat
    18601 cggcagcgaa agtcggcaag attcaggttc ttcgcgtcat tgattgatcc agcgtgttca
    18661 gctaagcgcc tatatagtgc ttgaccggga tcggcatcca aaccatagcc gcctttacgt
    18721 gcaccagcgg gaaccgcctt gcccacgtaa atgggccaac ggtattgatc ggctctattt
    18781 tgctcggcga tatccgcgta aagcggaaaa tcgccttcgt agtataaggc gtagatgccg
    18841 gcaccgatga aaggtttatc tggtggcagt agatgaactt tttgctgcag cattgcttcg
    18901 gcaacactct cgcccaagtt cgtcttatcc agtgggttga atgggggtat ccgctttggt
    18961 tgctttgcca tcgtaatcct cacgttactt gtttcaaaat gcttttgcca acggctgcgg
    19021 ccagttcgac gggtacggcg tttcctatct ggcgcatagc ctcggtccat gaaccgctga
    19081 agacatagtc atcggggaac gtctgaagcc gtgcgctctc acgtaccgtg tagtaacgat
    19141 aggacccatc cggcaatgcc atcatgttct ctccacccgg aacaccgtgg tcgccagcct
    19201 tgagggcttt cgacggtaaa tccagtgaac ttccggtatg accgtggtat gggcgagctc
    19261 cgtcacggaa ctcgtgattc gggacggtgt cgctcttgtt tctggggtct ggaagtccgc
    19321 gaagcgcatc ccgtaccgtc ttgcaccgtt cgccgggcgg gggaaacaag ctgaagtccg
    19381 actcaagacg acggatgcgt gccgcaagtt ttgcgtgctt ggccggaagt ttgctcttgg
    19441 gaacgcgatg ctcttcccaa tactctcccg aaacccactg atcccagaga aggcgatcaa
    19501 gtgagtgagt aggttccgga aatgaccatt ccttctgaag atccgaacgg aatccgacga
    19561 tgaagacgcg gtgtcggtgc tgcgaaacgc catattcggc tgcatcaagc aggcgataca
    19621 ccactctgta cgaaagatct gcttctttcg ctcccgtatg gtgacgttca agcttagaca
    19681 ggtgcgattg ccagtcttca tcacccttgc ggatgatcat gggatacgac aactgaagaa
    19741 tgatgtagtt gaagtagctc gaaaaggtct gccgaagcaa tcccttcacg ttctcaaaaa
    19801 caaaacactt tggacgcagt tcacgtaccg cgcgaactgc ttcggggaac atgtctctct
    19861 tgtcttgata tgcccgatgt ttcccaccaa gagagaaggg ttggcatggc ggtcccccgg
    19921 ccaccatgtc aacgtgatcg gaaagatcaa gataacttat ggtccgcacg tcgtcgggaa
    19981 atagccgcca tcccttagtc aacggatgcc caagagactc gttcaaatgg atagtcgagc
    20041 aggcgtgttc atcccactcg atcagcgcgg agtggtgcca tccgctctgc tctaagccga
    20101 gcgcgagacc gcctgccccc gtgaacaact cgattgatga tcgtccgtta ttcatttctc
    20161 atccagaaat cgcttgaccc gattccttac aaccgtttct ttcttgactt ggcactccca
    20221 aacaaccatg acccgccatc ccagcttccg aaattgacgt ctgacttctg cgtctcgttc
    20281 tttattcttc gcgagttttg gttcccaaaa ctcacgcttc gtgcgcggct gccgatactg
    20341 gcggcaaccg tgctggtgcc agaagcaccc atgcacgaat attactttct ttcgggggcg
    20401 aaaagcaaga tcaggattac ctggcaagtc ccgcgcgtgg aggcggtagc ggtaacccat
    20461 gccgtgaacc aaacgacgaa cgatcatctc cggcttggtg tcagcattgc ggatacgaga
    20521 catacgctca cttcgctcag catcggtcaa tggatcatag cgcatcatct tctgtttttc
    20581 tctctgttcc aacgaaaaac tcaccgggag cagacctgcg ggctgctatc cggtggagcg
    20641 acaagtataa cgaatactct ctttaaggaa gcaacgttca aacacgattc acaggatcgt
    20701 tgtcttccta tgatccgacg cggatcggcc tataccgctc aaatcgcctc gtttcgggtc
    20761 gccgcgtcgc ggggcgtttc gacggcgatc tcccgcaggc cggtgatcgc cgtgacggat
    20821 ttctcgggga ggaggatccg gctcggcgtg agggacaccc ccagccggtt cagttcgagg
    20881 aggcggtgga agagcgtctg gtgttcgagg tcgaggtcgc cgtagccagc cgaataccgc
    20941 gccgtcagga gggtgcgtcc ctcccggcgc agggcgcggt tttcgacggc cgtgatccag
    21001 tccagggcgg cgtccgtcat ttcgctcgcc gtcgcgtcca ggacgacgcc gcgggtggcc
    21061 cgcccctccg ccatgtccgc gcggattgcc gccatgatcg cctcccccgc cgtcgccccc
    21121 atcagcagga cctcccggct cgtgcccagg aagtcggcca gccggcggct cgccagcacc
    21181 gccccgccgt ccagcacgat ccggtcgggc ccccgttcga ccacggccag gcggcgggac
    21241 gcccccgcga ggcggatcag gtcggcggcc gcgtcgatcg ccgcctccgt ctcccgtcgc
    21301 tgtgcctccg acagccgggt ggaggccgac cggaagccga ggcgccggta gatcgccgcc
    21361 cggggcggcg cgatcgcgat ccgctggaaa acggtcacgg gggccatcgg gccggttcct
    21421 atccctccac gtccttgaac gcgaggtcct ggtaggcgtg ggtcagggtg tggcagaagg
    21481 cgtcccagcg acggcagacg tcgagggtgg ccgcgtccat cccgaacagg tgcgcatgcg
    21541 cgaggagcgc ctcggcctcc gcggcgagcg gcgcgacgac ggcctcctcc agcccgtcca
    21601 ggaggtcccg gacgaaggcc gccagcgcgt accggtcttt cacccgctgg aggtcccgga
    21661 agatctgggt gagcaggacg ttgcgcggcc cctcccagag ttcgttgacg gccgaatccc
    21721 ggtagaggcg ggggagcgag gagaagtcct ccatgacgcc gtggccgccg aagacggaca
    21781 tggccgcgcg gatcacgtcc gtgcagtccc acgacgcggc cagcttctgg agcatgatga
    21841 gttcccggat gacgaagcgc ttccgccagg ccgtctccgt ctcgccggta tcgagtccgc
    21901 ccttgaggcc cccctcgagc gtgagaaagt cccggtagag cttgaaggcc ccggccgtcg
    21961 tccgcttcgc cgcatggtcc atggcggcga gctggcccgc caccatcggg aaccggccga
    22021 tcgtgacccc gaaggcgtcg cggaactccg cgtactgctt ggcctcgcgg accgcccggg
    22081 tcatgaaggc ggccgccgag agccccacgg tgaggcgcga ataggtcagg acgatcccga
    22141 cgacgttcgc gacccccttg tccaacggcc ccacgggata ggccacggcc ccgttgaagg
    22201 tcagctcgcc cgtggtcagt tcgctcgtcc cgatcttcca cttgatccgg tcgatggtgt
    22261 acccgttgcg gatctccttc gccttgtccc ccggtagcca ggaggggatg acgaagaggc
    22321 cgaccttctc cgaccccttc ggcttggcgg tgacgacggc gtagtcggcg tgggtggcgg
    22381 agcagaagaa cttcgttccg tagagccgcc agacgccgtc ctcctcgacg gcctcgagga
    22441 ggttggcggg aacgtccgag ccgccctgga tctcggacag gtactgcgcg ccgatggcga
    22501 agccgtcccc gtcgccctcc ttgcagtgcg cgaggatggc cttcgtctcg ggggtatcgg
    22561 cgaacttctc gagaatcatg acgaggcctt ccgtgcaggt gatcgggcag gcgacacagg
    22621 cctcgccgtt ctggtagatc aggtacatct tgatcagctt ttcccagggc gaggtctttt
    22681 ccgagtagaa ggcctccgcg aaaacctccc gctccagggt ctcggtctcc ccggggcggc
    22741 agatgcggtc gatccggcgg tggtggccgt cgtagtgcat catgaacggg cggttctccg
    22801 gcaaggcgat ccgctcggcc aggtcccgcc agcggtagga ggccttccgc gaaaccgcgc
    22861 gcgcggcggc gtccacccgg tccgcctcgt ctcccgtgaa acggcggacg accttctgaa
    22921 tgaaggcgtc ggcggcgtag tagtccaccg cctgacgcca ggccaaaaat gcatcgaaat
    22981 tatagggatt gttgcgatcc ggtccggaca tggcgacctc cttgagacga tgagggctgg
    23041 cttgcaggtt tgggggtgcg gcggcatc
//
LOCUS       DAOOLJ010000026        22178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2271_length_22178_cov_17.904669, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000026 DAOOLJ010000000
VERSION     DAOOLJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 22178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22178
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2271_length_22178_cov_17.904669"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLW16_05995"
     CDS             complement(<1..299)
                     /locus_tag="PLW16_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70490.1"
                     /translation="MDRPLIIPIFIPYQGCPRRCIYCNERITAGSPQPPPTATALKKQ
                     ILTGRHRTRRHPLRVEIAFYGGTFTGLDREAQAALLAPATACLREGLVQGIRISTR"
     gene            complement(310..1017)
                     /gene="rnc"
                     /locus_tag="PLW16_06000"
     CDS             complement(310..1017)
                     /gene="rnc"
                     /locus_tag="PLW16_06000"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB70491.1"
                     /translation="MKEERIRGLKRCEETLQYRFRDLSLLDHALTHRSYANENAALEI
                     KDNERLEFLGDAVLELCISDLLMRRFPDHAEGDLTKLRAAVVNERPLAELALRLDIGA
                     HLLLGRGEAASGGSAKPSLLANALEAVIAAVYLDSTYLETLAFIGRLFSAAIAEGTRA
                     LFYQDFKSALQEICQERYHAIPRYTVEAAEGPDHDKVFEIRVAVADVLTASGRGRTKK
                     EAEQEAARKALLALEAA"
     gene            complement(1014..>1768)
                     /locus_tag="PLW16_06005"
     CDS             complement(1014..>1768)
                     /locus_tag="PLW16_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70492.1"
                     /translation="RNGYREVVLTGIHLGAYGQDLAPATDLARLLRRAEADTPVERLR
                     LSSLEPLEAEGVAETIRSGGRICPHLHIPLQSGDDGILAAMNRHYDSAFFAGLIHRLV
                     EAIPDLGVGVDVLAGFPGESENAFLRTVALLEALPVAYLHVFPYSRRPGTAAVDLPGQ
                     VPEAEKKKRCETLRALGQRKRQAFAERFVGRTLSVLIEDKRDPATGLERGFSENYIPV
                     LVEGGPGTAARRIVPVRTERASAGRLFGKVSP"
     assembly_gap    1769..1868
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1869..2057)
                     /locus_tag="PLW16_06010"
     CDS             complement(<1869..2057)
                     /locus_tag="PLW16_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPB70493.1"
                     /translation="MRISVVTLGCKVNQCESAALTEAFAARGHTSVGPDEPVDICVVN
                     TCTVTGRTDYQSRQIIRKV"
     gene            2317..2670
                     /locus_tag="PLW16_06015"
     CDS             2317..2670
                     /locus_tag="PLW16_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70494.1"
                     /translation="MEQVFHYLSTPVGILVAIFGALLILYFMLKKLVKLALLVVLVLF
                     GLAGYYYFQDPATMPQKMKQTLQGAKTKTEHAVEKGKGAYRTGREIYGKGKELSQTLD
                     KLKGTPRQPPPLERK"
     gene            complement(2674..3084)
                     /locus_tag="PLW16_06020"
     CDS             complement(2674..3084)
                     /locus_tag="PLW16_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB70495.1"
                     /translation="MYSKKVVIRYTADIVEEPLIYQLVKRYDLVFNILKARIFPRREG
                     VIVLELTGTRENFDLGIRFLKEKGLKVEPLSKSVSQNPEKCVHCGACTGFCPTSALYL
                     DRTTMKVLFEPEKCNGCELCVSACPSRAMEVNLF"
     gene            complement(3200..3676)
                     /locus_tag="PLW16_06025"
     CDS             complement(3200..3676)
                     /locus_tag="PLW16_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB70496.1"
                     /translation="MAIRVFIHGLESTSQGTKGVYFRTRYPDMIVEDFLGNLDERMAK
                     LRTLLADRTDLVLVGSSYGGLMAALYACENEAAVRRLVLLAPALDLAAFAPCRRCRLA
                     IPVTLYHGSRDDVVPPDAVKAIAQEVFTRLDYRRVDDDHPLRETFPTLDWDTLLRP"
     gene            3809..5059
                     /locus_tag="PLW16_06030"
     CDS             3809..5059
                     /locus_tag="PLW16_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPB70497.1"
                     /translation="MAVIVPFRAIRPRKPFVKDVASYPYDVLNAEEAGRLARANPSSF
                     LHVEKAEVDVPDFEGIDDARIYASARKRLEAMLHDGVLFQEAEPCLYLYRQVMDGRPQ
                     YGLVAGARVDEYEAGLIRKHELTRADKETERIRHVDEVNAHTGPVFIVYRARKAVDAL
                     LARAAEGEPEYDFTADDGIAHTVWVIRDAGVVDALKRQFAQVEALYIADGHHRAAAAA
                     AVARMRRERNPGHRGREGYNAMLGVLFPHDQVRIMDYNRVVRDLRGRTPQAFLAAIEE
                     NFAVSPDFQDKSPRERHAFGMYLDGRWYRLAARPGTYDAGDPVAALDVSILQENLLRP
                     LLGIDDPRTDGRIDFIGGIRGMEALERRVDREGYAVAFSLFPPTLDELMGVADAGRTM
                     PPKSTWFEPKLRSGIFVHSLDDAE"
     gene            5161..5979
                     /locus_tag="PLW16_06035"
     CDS             5161..5979
                     /locus_tag="PLW16_06035"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HPB70498.1"
                     /translation="MGSQGVRGSGRKVTAPADIQPLLKAGETLDILLDGRLRIMQPEK
                     GYRFSIDALLLAHFVRLKDRASVLDLGTGSGVIALLLALRWRYAQVVGIEIQPELADL
                     ARRNVQANGLDDRIEIRCGDVRQPAGAIPERACDAVVFNPPYRRLDAGRINDDPQRAR
                     SRHEILGSLQDFLRAAAFALKAKGSVSLIYPSSRASELMVRMRAARLEPKRLLPVYSH
                     AASEGEFVLVEGIKAGGEEMKLLPPLVLYGGDGRYTASVEAIFSDLATLSDDAG"
     gene            complement(5942..6439)
                     /locus_tag="PLW16_06040"
     CDS             complement(5942..6439)
                     /locus_tag="PLW16_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPB70499.1"
                     /translation="MRKRTRPGKLQRLGDVLEKVLKKQAVPLPARDQELITAWRKAVG
                     QQVAAQTFPDRLRRETLYVRVSTSVWMHQLQFMKQDILPRINAILKQREVKALHFSLG
                     EIPPMPASPGDAPQEGPVRLKARDRKMIEESTARLRDPELREIFQRVMIKEISRRRLK
                     ESQDR"
     gene            complement(6537..7538)
                     /gene="msrB"
                     /locus_tag="PLW16_06045"
     CDS             complement(6537..7538)
                     /gene="msrB"
                     /locus_tag="PLW16_06045"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPB70500.1"
                     /translation="MGKADPVIRPATFAGGCFWCVQSDFRKAPGVVKVVSGYTGGEGE
                     TPTYETYAESGHVEAVQVWYDPARTSYERLLDVFWRHIDPTDASGQFADRGPHYRSII
                     FYHDAEQRRAAERSRAALGQSGRFSRPIATEILAFTRFYAAEAYHQDYDRKNPLRYRA
                     YRYGSGRERFLEETWKTDAASETQPETPDVKPDEATLRKRLTALQYRVTQEEGTEPAF
                     QNAYWDNKREGIYVDVVSGEPLFSSRDKYDSGTGWPSFTRPITPGRLVEKEDRGAFMR
                     RTEVRSRQADSHLGHVFPDGPKPTGLRYCMNSAALRFIPKEDLDKEGYGAFRKLFEP"
     gene            7867..9069
                     /locus_tag="PLW16_06050"
     CDS             7867..9069
                     /locus_tag="PLW16_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB70501.1"
                     /translation="MLTDVQLDRYAEVLLWGLKTARKGRFRKHDIVLVQYDPAAVRLA
                     EILHAKILARGMNPVQRVGLTVKMERGFYAYAGARQLTFLAPGDRELYENVHGRIFLR
                     APDSVTHLKDVDPARIGKVLVSRKPLRDILDRREERGLYGWTLCILPTAELARQARTT
                     LKTYEAQLIRACYLDREAPVAEWERIFRSMQEIKQWLNALPVKAFHVEGRNIDLTVTP
                     GARRKWAGVSGHNIPSFEVFLSPDWRGTEGTYYANQPSFRSGNYVEGVHLTFSRGSVV
                     KIEAAEGEAFVKKQLAIDRGAGRLGEFSLTDRRFSRIDRFMAETLFDENHGGKYGNCH
                     IAVGASYSDTYRGDPSTLTREKKAELGFNDSALHWDLVNTEDKTVTAHLTTGEKVLIY
                     ANGCFLNG"
     gene            complement(9154..9477)
                     /locus_tag="PLW16_06055"
     CDS             complement(9154..9477)
                     /locus_tag="PLW16_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPB70502.1"
                     /translation="MNKKLYVGNLTHNVTEDDLKANFSQCGNIVSLNIIRDKFSGQSK
                     GFGFVEMETEEAAQEAIKKFSGGQLDGNTIVVNEAKPKKDTGGSGPRRFGGGGGGYRG
                     GGRRY"
     gene            complement(9847..10653)
                     /locus_tag="PLW16_06060"
     CDS             complement(9847..10653)
                     /locus_tag="PLW16_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HPB70503.1"
                     /translation="MNMTVSIVQMAVATADPEANLKKGESWIAEAARRGSELVCFPEL
                     WTTGFNWAENERLAPAHEQVIARIGALAARYGLWINGSLIALNERGQVSNTSVLFDDA
                     GRLAATYRKTHLFTLIHEEAHMAAGDALCTAETPWGKAGLAICYDIRFPELFRTYALK
                     GTRFILTPAAFPAPRRQHWKTLLRARAIENQMFMIGVNQSGREDFGLDGTVAYGGASA
                     IIDPWGETVIEAGDQGEALLTATIDLDRVEEVRTRMQVLGDRRPDLYELG"
     gene            10840..11190
                     /locus_tag="PLW16_06065"
     CDS             10840..11190
                     /locus_tag="PLW16_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPB70504.1"
                     /translation="MALMDLIAQVKAHPEYHRVGMILCHNGVARGTSRNGAPVTRLEV
                     TADRDRLGEILAEMKARPGIIEVLAEVREGALQVGDDVMFVVVAGDFRENVFPVLMDA
                     VNAIKRDVTRKRER"
     gene            complement(11216..11746)
                     /locus_tag="PLW16_06070"
     CDS             complement(11216..11746)
                     /locus_tag="PLW16_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPB70505.1"
                     /translation="MEATINRSVLALIEGDITEQETDAIVNAANERLAGGAGVDGAIH
                     RAGGPAIMAECRQIGGCPTGQAVITTGGNLKARHVIHTVGPVYRGGTRGEAELLKSAY
                     LESLKLASEKGLKSVAFPAISLGIYGYPREEGARIALKTAIDYLKAHDGIARVHFVLF
                     GRDMYGLFARELRLLL"
     gene            complement(11750..13336)
                     /locus_tag="PLW16_06075"
     CDS             complement(11750..13336)
                     /locus_tag="PLW16_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB70506.1"
                     /translation="MSKWAGDALSRAVREGYRAGREDETLEPLVLVDERGNPIGRLKS
                     GDAVIFYNIRGEREIELTRSLTEPGFSEFPVHPDLALHFATMIRYREDLRVEVAFPPD
                     AVVADTLSETIARHGLKQIKITEAEKAVHVGFFLNGKRDEPFDGETRRIVATRKDVAL
                     FDKAPEMSIAAVADAVLDAIRDDTYTFIFANFPNVDVVGHIENEAAILQAIEAVDRNL
                     GRVVEAATRAGLTMVVSADHGTVEKWLYPDGTIDTGHTDSPVPFLLLDPDGPAPLRPS
                     GELTDIAPTVLERMGLPVPAAMTGRSLLAGPRSGQKPARRLLLLVLDGWGHAASGPGN
                     LIARAQTPNMDRLWQTVPHALLAAAGEAVGLPEGTVGNSEAGHLHIGAGRRIYADRVK
                     IDRAIADGSFFSNPAFLGTVRAARDAGTALHLMGIVSFFSSHGSLRHLFALMDLARRE
                     GVQNVFIHAMLGRRGEKPESGANYIREIETKCLADGLGRVASVIGRYWSMDREENWNR
                     IEKTYRMLVYGEGTSVAERG"
     gene            13561..13956
                     /locus_tag="PLW16_06080"
     CDS             13561..13956
                     /locus_tag="PLW16_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70507.1"
                     /translation="MSKPKSPDEILQEEFLRERAEVLGRAGTRVQESLNALRRIGEEI
                     AAVEGRYEELAPVQAVSAAGCGESQAQRARLRREIDAQIHRYNRVREEAKLRYYYLIV
                     TREALGLRRHHWVESVYRIPPKKAAPQDG"
     gene            13956..14609
                     /locus_tag="PLW16_06085"
     CDS             13956..14609
                     /locus_tag="PLW16_06085"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPB70508.1"
                     /translation="MDIYTKQRKRMVETQLRSRGIRDERVLKALEAIPRHLFVDEALR
                     DQAYFDHPLPIDAGQTISQPYIVALMTAALELRGSERVLEIGTGSGYQTAVLAALADQ
                     VFSIERVAPLASRARKILDALDDYNVAIRIGDGTYGWREESPFDAIIVTAGAPRVPRI
                     LVEQLAVGGRLVIPVGDRHTQDLVRVIRQSEDAGDIKQEDLGGCRFVSLIGEHGWQT"
     gene            14659..15579
                     /locus_tag="PLW16_06090"
     CDS             14659..15579
                     /locus_tag="PLW16_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPB70509.1"
                     /translation="MKRTFVLLALLACTLACTGYLDRTETSRGVYHLVQRGETLSVIA
                     RTYRVTVQDLAEYNNITDPDMIVEGSALFIPGAVQIHDIDLPAIRESSPSVPEPVAAV
                     PPASPSPRTVPRTQPVPDVRAPSSGAAKPPPAADVSGKTRPAKDSGGAAVQARPSPPR
                     DAGPPTARPRPAPATPSEDIRFERQRFIWPVRGPVKTRFGIQPNGLNANGIEIDAPEN
                     TPVLAAAGGTVIFSDTLKEYGETVILKHPDDFKTVYIQLKSRRVQRDQQVKRGEIIAL
                     TGKPEKKREPYWNFEIRYRNKARNPMFFLP"
     gene            15634..16773
                     /locus_tag="PLW16_06095"
     CDS             15634..16773
                     /locus_tag="PLW16_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70510.1"
                     /translation="MFEQGPIRPPSEARSLLIRVTRNCPWNKCAFCHTYRGKAFSLRT
                     VAEIKEEITRIRQIADQIRQWSWRQGDGGRITDRLVNRIYSREDDVDDGYRSVAAWLY
                     FGATSVFLQDANSLIMKTDDLAEVLTFIKAQFPSVGRITTYARSHTAARRSVEDFVKL
                     REAGLTRIHIGMESGYDPLLAFIRKGVTAADHVTGGQRIVAAGISLSEYVMPGLGGRR
                     WSREHALATADAINRINPEFARLRTLHVVPGTDLHDLMTRGEFQPLSDEKILREIRCF
                     IEALNGIETTLVSDHILNLLEELEGKLPEDKARLLATVDRYFALPDEDRRIYRLGRRR
                     GVYRRLDDLADARVYRRLQDVVSAYHEGDPGRLDQHLDGIMNRYI"
     gene            16867..17352
                     /locus_tag="PLW16_06100"
     CDS             16867..17352
                     /locus_tag="PLW16_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPB70511.1"
                     /translation="MFKVGDLAVYPAQGVGVIEAIEHREFMGSKQPFYIMKIMGNGMK
                     IMIPTSSAESVGLRQVISQKEIPKIYEILRNKDAAIDKQTWNKRHREYLEKIKTGSVF
                     EIAKVLRDLFILKSDKNLSFGERKMMDTAKSLLIKEISVACNTEETKIEQDLKRIFPV
                     Q"
     gene            17507..18529
                     /locus_tag="PLW16_06105"
     CDS             17507..18529
                     /locus_tag="PLW16_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPB70512.1"
                     /translation="MIIRLVLILACSVSGYFIVYHYDWGFPTSLLGLVAGIIIALSVI
                     QIEQAIRKVSIRVIFGGVAGMFIGLLIAFLFAYGLSFVTNISEKRQIAPWIYALLTVT
                     MGYLGLVLGSKKIEEVHVFGLHAPKEPVDCRILDTSVIIDGRIADICDTGFIDGNFIV
                     PRFVLDELQYVADSSDSLKRSRGRRGLDILNRMQRASGISIEVLDQDYPRIKGVDAKL
                     VALAREKKGKIITNDFNLNKVAELQGIKILNVNELANAVKPVVLPGEAMTVKIIREGK
                     EPGQGVAYLDDGTMIVVDNAQRYQGQLVDVQVTSVLQTTAGRMIFSEMKGLAQDKKMF
                     ALGAKS"
     gene            18579..19781
                     /gene="ispD"
                     /locus_tag="PLW16_06110"
     CDS             18579..19781
                     /gene="ispD"
                     /locus_tag="PLW16_06110"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPB70513.1"
                     /translation="MNVTAIIAAGGAGRRMGTARPKQYLPLGDLPVIVHVLKTFSRVS
                     AVTEILLVVPRADVPHVRSEIVGRYGLGKVRRVIAGGRERQDSVAKGLAAVRAETTDI
                     VVIHDAVRPLATPALVEAAIAGAVCWGAVATGIPVRDTVKSTDAEGWVEETLPRERLC
                     LVQTPQAFRLEILRRAHERAAAEGFYGTDDAALVERTGTRVRMISGLARNIKMTTPED
                     LDWARWFLEKRGREMGEGATEMPERVGCGYDSHRLVADRRLILGGVDIPHPRGLLGHS
                     DGDALIHAVIDALLGAVGGGDIGRLFPDSDPAYRGVSSLTLLARVCSLVKEKRFEVVH
                     LDATILMEKPKLAVHIPAMIEHLSATLCLDAGRINIKAKTNEGMGFVGREEGVAAFAV
                     ATVRECGR"
     gene            19778..21232
                     /gene="gltX"
                     /locus_tag="PLW16_06115"
     CDS             19778..21232
                     /gene="gltX"
                     /locus_tag="PLW16_06115"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB70514.1"
                     /translation="MNEAAQPRVRFAPSPTGDLHIGNARTALFNWLFARHTGGAFILR
                     IEDTDRDRTEDVFIANLLEDLRWLSLDWDEGPDRVGACGPYRQSERLDLYREHLARLI
                     EAGWVYPCYCAEAELEAERAALLARRMAPRYLGKCRQLTVADRRRYEREGRRPAYRFR
                     VAEGPVYFTDLIRGEMSFQGEALGDFIVVRSNGIPAYNFACVVDDHLMAISHVIRGED
                     HLSNTALQCLLYRALEFNPPVFAHHALILAPDRAKLGKRHGAVSVRTFRERGILPQAL
                     SNYLAILGSSLTEKQEICTQESLVRDFSLDRTGRGGAVFDEAKLAWLNALYLKRMPLD
                     DLTARILPFLEREGMNVRRLDRRWLMALVEAVRDNCATLADVPRHLNVFFDERHTWND
                     AARTVLTDDNARRVVRLFRDLLLADLPPDEQVFARSLKALRKQAGVKGRALYLPLRAA
                     VTGATEGPELDRIMAALGWEAVVQRLDRVLRESF"
     gene            complement(21435..>22178)
                     /locus_tag="PLW16_06120"
     CDS             complement(21435..>22178)
                     /locus_tag="PLW16_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70515.1"
                     /translation="LFLDVLGNKELVDGYQIEDSIAQKYHLTPGSICPFTGLRVEILS
                     PQQIHAAEKAARRRVIKPKRTGPVEEKQKLQSPLAQKQKKKNLSTAGTKISGVAAGLK
                     ATRKSIHPEASHDSARGRSRSIQTPTRRDRTFKKEEGRMATAKKVQAKPAKAAVKAPA
                     KAKAAAKAPVKAAAKPTKAKAKAVAKAPAKAKASVKAKAVAKAPAKAKAKAPAKAKAV
                     AKAPAKAKAKAKAPAKAKAVAKKPAKAKK"
BASE COUNT         3870 a         6806 c         7324 g         4078 t
ORIGIN      
        1 cgggtggaga tccggatgcc ctgcacgagg ccttcccgga gacaggcggt ggcgggggcg
       61 agcagggccg cctgggcctc ccggtccagg ccggtgaagg tgccgccgta gaaggcgatt
      121 tcgacccgga gcgggtgacg ccgcgttcgg tggcggccgg tgagaatctg cttcttcaag
      181 gcggtggcgg tcggaggcgg ctgggggctg ccggcggtga tgcgttcgtt gcagtagatg
      241 cagcgtcggg ggcaaccctg ataggggatg aagatcggaa tgatcagggg cctgtccatg
      301 cgcaaccctt caggcggcct ccagggcgag cagggccttg cgggccgctt cctgttccgc
      361 ctctttcttg gtccggcccc ggccgctggc ggtcaggacg tcggccacgg ccacgcggat
      421 ctcgaagacc ttgtcgtgat ccggtccttc cgccgcctcc acggtgtaac ggggaatggc
      481 gtggtagcgc tcctggcaga tctcctgcag ggcgctcttg aagtcctgat agaacaaggc
      541 ccgcgtccct tcggcgatgg cggcggaaaa caggcggccg atgaaggcca gggtttccag
      601 ataggtgctg tccaggtaga cggcggcgat cacggcctcc agggcgttcg ccagaaggga
      661 gggcttggcg ctcccgccgc tggccgcctc gccccggccc aacagcaggt gggcgccgat
      721 gtccagccgc agggccaact cggcgagggg gcgctcgttg acgacggccg cccggagctt
      781 cgtgaggtcg ccttccgcat gatccgggaa gcgcctcatg agcagatcgc tgatgcacaa
      841 ttcgagaacg gcgtccccca ggaattccag acgctcgttg tccttgattt ccagggccgc
      901 gttctcgttg gcgtaggagc gatgggtcag ggcgtgatcg agaagcgaga gatcgcggaa
      961 acggtactgg agcgtctcct cgcaccgttt gaggccccgg atgcgctcct ccttcatggc
     1021 gacacctttc cgaacaaccg gcccgcggac gcccgttcgg ttcggaccgg aacgatgcgc
     1081 cgggccgcgg tgcccggtcc gccctccacc agcaccggga tgtagttctc cgagaagccc
     1141 cgctccaggc ccgtggccgg gtcgcgcttg tcctcgatca gaacggacag ggtccggccg
     1201 acaaagcgct ccgcgaaggc ctggcgcttt ctctgaccca gcgcccgcag cgtctcgcag
     1261 cgtttcttct tctccgcttc ggggacctgg ccgggcagat ccaccgccgc cgtgccggga
     1321 cggcgggagt aggggaagac gtggagatag gcgacgggca gggcctcgag cagcgccacc
     1381 gtccggagga aggcgttctc cgattcgccg ggaaagccgg ccagaacgtc gacgccgacg
     1441 ccgagatcgg ggatcgcctc gaccaggcgg tggatcagac cggcaaagaa ggcgctgtcg
     1501 tagtgccggt tcattgcggc cagaatgccg tcgtcgccgc tctggagcgg gatgtgcagg
     1561 tgggggcaga tccggccgcc ggagcggatc gtctcggcaa cgccctcggc ctccagcggt
     1621 tcgagggagc tgaggcggag tcgctccacc ggtgtgtcgg cttccgctcg ccggaggagg
     1681 cgggcgagat cggtggccgg cgccagatcc tggccgtagg ccccgaggtg gatgcccgtc
     1741 aggacgacct cccgatagcc gttgcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnga ccttccggat gatctgccgg gactggtagt cggtccggcc ggtcacggtg
     1921 caggtgttga cgacgcagat gtcgaccggc tcgtccggcc cgacggacgt gtgtccccgg
     1981 gccgcgaacg cttccgtcag cgcggccgat tcgcattggt tgaccttgca cccgagggtg
     2041 acgacggaga tcctcattcc gcgatggatt ccttccgatt cgacggtgca tcctgccggc
     2101 cgggaccccg ggccggaatc ggcaggccgc ggaatcgccc cgccttgccc ggtcgggatc
     2161 cgcgcctgcc gcgggtcggc ttcaaactct aaagagccgc tccgcttttg tcaaggcctt
     2221 aacccggcga ccgcggtcct ttccggatcg aaaggagtac tggattttgt ctgcggattc
     2281 cgttaaaatc cccggccata ctcaggaggg atgatcatgg aacaggtttt ccactacctt
     2341 tcgaccccgg tcggcattct ggtggcgatc ttcggcgccc tcctgatcct ctatttcatg
     2401 ctgaagaaac tcgtcaagct ggccctgctc gtggttctcg tcctcttcgg tctggccggc
     2461 tactactatt tccaggatcc ggccaccatg ccccagaaga tgaagcagac cctgcagggt
     2521 gccaagacca agacggagca cgcggtggaa aaggggaagg gcgcctatcg gacgggccgg
     2581 gaaatctacg gcaaggggaa ggaactctcc cagaccctcg acaaactcaa ggggacaccg
     2641 cggcaaccgc cgcccctgga gcgtaaatga tccctaaaag agattgacct ccatcgcccg
     2701 ggagggacag gcggacacgc acagctcgca cccgttgcat ttctccggct cgaagaggac
     2761 cttcatggtg gtgcggtcga ggtacagggc gctggttgga cagaaccccg tgcaggcgcc
     2821 gcagtggacg cacttctccg gattctggct gacgctcttg gacaggggtt cgaccttcaa
     2881 ccccttttcc ttcaggaaac ggatccccag atcgaaattt tcccgggtgc ccgtgagctc
     2941 caggacgatg acgccttccc ggcggggaaa gatacgggcc ttcaggatgt tgaaaacgag
     3001 gtcgtaccgt ttgacgagtt gatagatgag tggttcctca acgatgtcag cggtatagcg
     3061 gatgacgact ttcttggagt acatggattt ccttccgggt gggcatgacg ccgcagggtc
     3121 tcctctcgcg gccggtcttc aatttgccta ataaggacgg catattaggg gatcggggga
     3181 ggcttgtcaa gcaaataaat caagggcgaa gcagcgtatc ccagtccagc gtggggaagg
     3241 tttcgcgcaa ggggtggtcg tcgtccaccc ggcggtagtc gaggcgggtg aagacctctt
     3301 gggcgatggc cttgacggcg tcggggggga cgacgtcgtc ccgggaaccg tggtacagcg
     3361 tgacggggat cgccagacgg cagcggcgac agggggcgaa ggccgccaga tcgagggccg
     3421 gcgccagcag gacgagccga cggacggccg cctcgttctc gcaggcatac agggcggcca
     3481 tcagtccgcc gtaactggag ccaaccagga cgagatccgt ccggtcagcg agcagcgtcc
     3541 gcaacttcgc catccgttcg tccaggtttc cgaggaagtc ctcgacgatc atgtcgggat
     3601 agcgggtgcg gaaataaacc cccttggtgc cctggctcgt gctctccagt ccgtggatga
     3661 aaacgcggat cgccattgct cggaatcctt tcttgaccat tttggaaaaa gaccatagac
     3721 tcgccgcctc ggaaatgcaa gcgctttgcc gggacggatt cgcgtggggc gcctgcacga
     3781 cacgttttgc cgtcaggagg gcatgattat ggccgtgatc gttcccttcc gggccattcg
     3841 gccccggaag cctttcgtga aggacgtcgc ctcttacccc tacgacgtcc tcaatgccga
     3901 ggaggcaggg cggctcgccc gcgccaatcc ctcgagcttt ctgcatgtgg aaaaggcgga
     3961 ggtcgacgtc ccggattttg agggaatcga cgacgcccgg atctacgcgt cggcccggaa
     4021 gcgcctggag gcgatgcttc acgacggcgt tcttttccag gaggccgagc cctgccttta
     4081 cctctaccgg caggtgatgg acggccgtcc ccagtacggt ctcgtcgccg gcgcccgcgt
     4141 ggacgaatac gaggcgggcc tcatccgcaa gcatgaactg actcgggcgg acaaggaaac
     4201 cgagcggatc cggcacgtcg atgaagtcaa cgcccatacg ggtcccgtct tcatcgtcta
     4261 tcgcgcccgg aaggccgtcg atgcgctgct ggcccgcgcg gccgaggggg agccggagta
     4321 cgatttcacc gcagacgacg gcatcgccca caccgtgtgg gtgatccgcg acgccggggt
     4381 cgtggacgcc ctgaagcgtc agttcgcgca ggtggaggcc ctctacatcg ccgacggcca
     4441 tcaccgggcg gcggcggcgg cggcggtggc gcgcatgcgc cgggagcgca atccgggaca
     4501 tcggggccgg gagggctaca acgccatgct gggggtgctc tttccccacg accaggtccg
     4561 gatcatggat tacaaccggg tcgtccggga cctccggggg cggacgccgc aggcgttcct
     4621 cgccgccatc gaggaaaact ttgcggtcag cccggatttc caggacaagt ccccgcggga
     4681 gcgccatgcg ttcggcatgt atctcgacgg ccgctggtac cgccttgccg cccgcccggg
     4741 aacctatgac gccggcgatc cggtggccgc cctggacgtg tccatccttc aggagaatct
     4801 gctcaggccc ctgctcggca tcgacgaccc gcgcaccgac gggcggatcg acttcatcgg
     4861 cgggatccgc ggcatggagg cgctggaacg gcgcgtggat cgtgaggggt atgccgtcgc
     4921 gttctccctc ttcccgccca ccctcgatga attgatgggg gtggccgacg cgggacggac
     4981 catgcccccg aagtcgacgt ggttcgagcc caagctccgc tcggggattt tcgttcacag
     5041 cctcgatgac gcggagtgaa gggggaagcg tcttgaaaat tccgctccgt gtgcgcatcc
     5101 cgaaaaggcg ccgatgcggg cccggaggga gcggggcttg cgccagataa ggaggaatcc
     5161 gtgggaagcc aaggggtgag gggcagtggg cggaaggtca cggcacccgc ggacattcaa
     5221 cccctgttga aggcggggga aaccctcgac atcctcctcg acggccggct ccggattatg
     5281 cagccggaga aaggctaccg gttttccatc gatgccctgc tcctggccca tttcgtccgc
     5341 ctgaaggatc gcgcctccgt cctggatctg ggcaccggca gcggcgtgat cgccctcctg
     5401 ctggcgctcc ggtggcggta cgcgcaggtt gtcgggatcg agattcagcc ggagctggcg
     5461 gatctggccc ggcgcaacgt tcaggcaaac ggcctcgacg accgcattga aatccggtgc
     5521 ggggacgtcc gccagcccgc cggcgcgatt ccggagcgcg cctgcgatgc ggtcgtcttc
     5581 aaccctccgt accggcgcct cgatgccgga cggatcaacg atgatcccca gcgggcccgg
     5641 tcccgtcacg agattctggg atccctccag gactttctgc gggcggcggc atttgccctg
     5701 aaggcgaaag ggtccgtcag cctgatctat ccgtcctccc gcgcgtcgga actgatggtg
     5761 cggatgcggg ccgcccgtct ggagcccaag cgcctgctcc cggtctattc ccatgcggcg
     5821 tccgaaggtg aattcgtcct ggtggagggg atcaaggcgg gcggggagga gatgaagctt
     5881 ctgcctccgc tcgttctcta cggcggcgac gggcgttaca cggcctcggt cgaggcgatt
     5941 ttcagcgatc ttgcgactct ttcagacgac gccggctgat ctccttgatc atcacccgct
     6001 gaaagatttc cctgagctcc gggtcccgga gccgggcggt gctttcctcg atcattttcc
     6061 ggtcccgggc cttgagccgg accggccctt cctgcggcgc atccccgggg gacgccggca
     6121 tgggcgggat ctcgccgagc gagaagtgga gggccttgac ctccctctgc ttcaggatgg
     6181 cgttgatccg ggggaggatg tcctgcttca tgaactggag ctggtgcatc cagaccgagg
     6241 tggagacccg gacatagagg gtctcgcggc gcagacggtc gggaaaggtc tgggcggcga
     6301 cctgctggcc gacggccttt cgccaggcgg tgatcagctc ctggtcccgg gcgggcaggg
     6361 ggacggcctg cttcttcaag accttttcga ggacgtcgcc gagccgctgc agctttccgg
     6421 gtcgggttct cttgcgcatg gcctttactg gcacagatcg ggacctttgc gcaaccggaa
     6481 aatccggaca cgtcaggatc gcgatcccga tccgtccggg caggggatcg tccgcctcaa
     6541 ggttcgaaca gcttccggaa ggcgccgtag ccctctttgt ccaggtcctc cttcgggatg
     6601 aatctcaacg cggccgagtt catgcagtaa cgcagaccgg tgggcttcgg cccgtccggg
     6661 aagacgtggc cgaggtggga gtccgcctgc cggctcctga cctcggtgcg ccgcatgaac
     6721 gcgccccggt cctccttttc gacgagccgg ccgggcgtga tgggtcgggt gaagctgggc
     6781 cagcccgtgc cggaatcgta cttgtcccgg gaactgaaga ggggttctcc ggaaacgacg
     6841 tccacgtaga tcccctcccg cttgttgtcc cagtacgcat tctgaaaggc cggttcggtg
     6901 ccctcctcct gggtgacccg gtactggagc gccgtcaatc gttttcgcag ggtcgcctcg
     6961 tcgggtttga cgtcaggcgt ctccggctgt gtctccgaag cggcgtccgt tttccaggtc
     7021 tcttccagaa accgttcccg tcccgatccg tagcgatagg ccctgtagcg gagggggttt
     7081 ttccggtcgt agtcctggtg gtacgcttcc gcagcgtaga agcgggtgaa ggcgaggatc
     7141 tccgtggcaa tcggtcggga aaaacgcccg gattgcccca gggccgcccg ggaccgttcc
     7201 gccgcccgcc gctgctccgc gtcgtgataa aagatgatgc tccggtaatg gggcccgcga
     7261 tccgcgaact gccccgaggc gtccgtgggg tcgatgtgcc gccagaacac gtcaagcagg
     7321 cgctcgtagg acgtccgcgc ggggtcgtac cagacctgaa cggcctcgac atgcccggat
     7381 tccgcgtagg tttcgtaagt gggggtttcc ccctcgcccc ccgtatagcc ggagacgacc
     7441 ttcacgaccc cgggcgcctt ccggaagtcg gactgcacgc accagaaaca gccgccggcg
     7501 aacgtggccg gccggatgac gggatccgct ttccccatgg ggttcacctc cgtggattga
     7561 atggggccct tgtcgcaccc cgggagcatc aagaatgcga tcgtcgccag gatcaggatc
     7621 ttcttcaccg ctttccctcc cgcttccacc ggcggttaag ttaacgacga tcggccgatt
     7681 gtcaaacccc ctcccgtggg ggtggaagag gcgggatttg ccattccgta caccgaattt
     7741 cgtcttttca ttggggaacg gatggtatat acaggtccgc gatcccggtg gagcgggagc
     7801 gcacgcccgg atgcggaccg gaacgacggg accgggaaac ggcggttgcc ggatggggag
     7861 gaaacgatgc tgacggatgt gcaactggat cgctatgcgg aggtgctcct ctgggggctg
     7921 aagaccgccc ggaagggccg tttccggaaa cacgatattg tcctcgtaca gtacgacccc
     7981 gccgccgtga gactcgcgga gattctgcat gctaaaatcc tggcgcgggg gatgaatccg
     8041 gtccagcggg tcggactcac ggtgaagatg gagcggggct tctacgcgta cgccggtgcg
     8101 agacagttga ccttcctcgc gccgggcgac cgcgaacttt acgaaaacgt ccacggccgg
     8161 attttcctgc gggcgccgga ttccgtgact cacctcaagg acgtcgatcc ggcgcggatc
     8221 ggaaaggtcc tcgtttcgag aaagcccctg cgcgacatcc tggaccggcg ggaggagcgg
     8281 gggctgtacg gctggaccct ctgcatcctg cccacggcgg agctggcccg ccaggcccga
     8341 accacgctga agacctacga agcccagctc atccgggcct gctacctgga ccgggaggcg
     8401 cccgtggcgg agtgggagcg gatcttccgg agcatgcaag agatcaagca gtggctgaac
     8461 gccctgccgg tgaaggcctt ccatgtcgag ggcaggaaca tcgacttgac cgtcaccccc
     8521 ggcgcgcggc ggaaatgggc gggcgtctcc ggccacaaca ttcccagctt cgaggtcttc
     8581 ctgtcgcccg attggcgcgg gaccgaaggg acgtattacg cgaatcagcc ttccttccgg
     8641 agcggcaact atgtggaggg ggtgcacctg acgttcagca ggggatccgt cgtgaagatc
     8701 gaggccgccg aaggcgaggc cttcgtcaag aagcagctgg ccatcgaccg gggtgccgga
     8761 aggcttggcg agttttccct gacggaccgg cgcttttccc ggatcgaccg gttcatggcc
     8821 gagaccctct ttgatgaaaa ccacggcggg aaatacggca attgccatat cgcggttggg
     8881 gcctcctact cggacaccta ccgtggcgat ccgtcgaccc tgacgcggga gaagaaagcg
     8941 gaactggggt tcaacgattc cgccctgcac tgggacctcg tcaacacgga ggacaagacc
     9001 gtaacggccc acctgaccac gggagagaag gtcctgattt acgcgaacgg ctgttttcta
     9061 aacgggtagc cggtttcgcg acgggtaaaa aaaaccgggg gacgcatggc agcgctcccc
     9121 ggttattgac cgtccggccc ggtggggacc gtctcaatag cgacggccgc ctccgcgata
     9181 gccgccccca ccgccgccga agcgacgggg tccgctgccc cccgtatcct tcttgggttt
     9241 ggcttcattg accacgatcg tgtttccgtc caactgaccg ccgctgaatt tcttgatcgc
     9301 ttcttgggcg gcctcctcgg tctccatctc gacgaatccg aaccctttgg attgcccgga
     9361 gaacttgtca cggatgatgt tcagggaaac gatattcccg cactgggaaa agttcgcctt
     9421 gagatcgtcc tcggtgacat tgtgggtcag attgcccacg tataatttct tgttcatgat
     9481 atcgactccc ttccaaaaag ttcgctgaca acggtctcac gtatgcagcc ggcggatttt
     9541 tccccccacc tgccgacgtg atcttccggc atgacgggca cccccctcgt gatgattcga
     9601 ggtcggatga ccatcgcgac ggaaataaaa aaccaccaat gctccggaga tgatggtggt
     9661 ttgccttgcg ctccaaactg tcctgaaccg acgggcggaa cctcctgaac gtccaccgga
     9721 ggatggaaaa caacccaaac tcgaagtcct tctatcggga acgctcgaga aagtcaagca
     9781 ctatctccgg agggcgaacg cgccccgccg ggccggccgg gaacggcgga gaccgcaagg
     9841 ccggatctat cccagttcgt acagatcggg ccttcgatcg cccaggacct gcatccgggt
     9901 gcggacctcc tcgacgcggt cgaggtcgat cgtggcggtc agcaacgctt ccccctggtc
     9961 tccggcctca atgaccgttt ccccccaggg atcgatgatc gcggaggcgc cgccgtaggc
    10021 gacggtcccg tccaggccga aatcctcccg ccccgactga ttgacaccga tcatgaacat
    10081 ctggttctcg atggcccggg cgcgcagcag ggttttccag tgctggcgcc ggggggcggg
    10141 aaacgccgcc ggcgtcagga tgaaccgggt gcctttcagg gcataggtgc ggaacagctc
    10201 cggaaagcgg atgtcgtagc atatggccaa cccggccttc ccccaggggg tttccgctgt
    10261 gcacagggcg tctcccgccg ccatgtgcgc ctcctcgtgg atcagcgtga aaagatgggt
    10321 cttgcggtag gtagcggcca agcggccggc gtcgtcgaag aggaccgagg tgttggacac
    10381 ctgcccccgt tcgttaaggg cgatgagcga gccgttgatc cagaggccgt accgggcggc
    10441 cagggcgccg atccgcgcga tcacctgttc gtgggcgggg gcgaggcgct cgttttcggc
    10501 ccagttgaag ccggtggtcc acagctcggg gaagcagacg agctcgctcc cgcggcgcgc
    10561 cgcttcggca atccagctct cgcccttctt caggttcgcc tccggatcgg ccgtggccac
    10621 ggccatctga acgatcgaaa cggtcatgtt catggcaggt ctccccggcg ccggtcccgg
    10681 gcgtggtcgg caggggaggg cgccgggggc cacgataggg aaagccggcc ggaatgtcaa
    10741 cgggaattcc gttccgatcg gcggcctttt gtgttaagag ccattcgaaa agcggtccct
    10801 ccgcgcgggg ggaaaggaag gcacggtgag gtgaggggca tggccctgat ggatctgatc
    10861 gcacaagtca aggcccatcc ggagtaccac cgggtgggga tgattctctg ccacaacggc
    10921 gtggcccggg ggacctcgcg gaacggcgcg ccggtgacac ggcttgaggt gacggcggat
    10981 cgcgaccgcc tgggagaaat tcttgccgag atgaaggcgc gtcccgggat catcgaggtg
    11041 ctcgccgagg tccgcgaggg ggcgctccag gtcggggacg acgtgatgtt tgtcgtcgtg
    11101 gccggcgatt ttcgcgagaa cgtcttcccc gtcctgatgg acgcggtcaa cgcgatcaag
    11161 cgcgacgtga cccgcaaacg ggagcgataa gcggccgtgc ggccggggcc gcgtactaca
    11221 ggagcaggcg cagttccctc gcgaagaggc catacatgtc ccggccgaag agcacgaagt
    11281 ggacccgcgc gatgccgtcg tgggccttga ggtaatcgat tgccgtcttc agggcaatcc
    11341 gcgccccttc ttctcgggga tagccgtaga tccccaggct gatggcggga aaggcgacgc
    11401 tcttcaggcc tttttccgag gccagtttca ggctttccag ataggcgctc ttgagcaact
    11461 ccgcttcgcc ccgcgtgccc ccgcggtaga cggggccgac agtgtggatg acatgcctcg
    11521 ccttgaggtt tccgccggtc gtgatgacgg cctgacctgt ggggcagccg ccgatctgcc
    11581 ggcattccgc catgatggcg ggtccgccgg cgcggtggat ggcgccgtcg accccggcgc
    11641 cgccagccag acgttcgttc gcggcgttga cgatggcgtc ggtctcctgc tccgtgatgt
    11701 ccccctcgat cagcgcgagg acggatcggt tgatcgtcgc ttccatggct tagccccttt
    11761 ccgccacgga agtgccctcc ccgtagacga gcatgcggta ggtcttctcg atgcggttcc
    11821 agttctcctc gcggtccatc gaccagtacc ggccgatgac ggaggcgacc cggccgaggc
    11881 cgtcggcgag gcacttcgtt tcaatctccc ggatgtaatt cgcgccgctt tccggttttt
    11941 ccccgcgccg gccgagcatg gcgtggatga agacgttttg gactccctcc cgtcgggcga
    12001 ggtccatcag ggcgaaaaga tgcctgagcg atccgtggga gctgaaaaag gagacgatgc
    12061 ccatcaggtg aagtgcggtt cccgcgtccc tggccgcccg gacggtgccg aggaaggccg
    12121 ggttcgagaa gaacgatccg tcggcgatcg cccggtcgat cttgacgcgg tcggcataga
    12181 tgcggcggcc ggcgccgatg tggaggtggc cggcctcgga attgcccacg gttccctccg
    12241 ggagtcccac tgcctccccg gcggcggcga gcagggcatg gggaacggtt tgccagagcc
    12301 ggtccatatt cggcgtttgc gcgcgggcga tgagattgcc cgggccggat gcggcatggc
    12361 cccagccgtc gagaacgagg agcagaagcc ggcgagccgg cttctgccct gagcgagggc
    12421 ccgccagcag cgatcgaccc gtcatggccg cgggcacggg aagacccatc cgctcgagga
    12481 ccgtgggagc gatgtccgta agttcgcccg acggacgcag gggcgccggg ccgtcgggat
    12541 caaggaggag aaaaggcacc gggctgtccg tgtggccggt gtcgatcgtc ccgtcggggt
    12601 agagccattt ttccacggtg ccgtgatcgg cgctgacgac catcgtcagg cccgcccggg
    12661 tcgcggcctc gacaacccgg cccagattcc ggtccaccgc ctcgatggcc tgcaggatgg
    12721 ccgcctcgtt ctcgatgtgc ccgacgacat ccacattcgg gaaattggcg aaaatgaagg
    12781 tatacgtgtc atcccggatc gcgtccagga ccgcgtcggc gaccgccgcg atcgacattt
    12841 ccggggcctt gtcgaagagg gcgacatcct tacgggttgc gacgatacgg cgcgtttccc
    12901 cgtcgaaggg ttcgtccctt ttgccgttca ggaagaatcc gacgtgcacc gccttttccg
    12961 cctccgtgat cttgatctgt ttcaagccat ggcgggcgat ggtttcgctc agggtgtcgg
    13021 cgacgaccgc gtccggcgga aaggcgacct ccaccctgag gtcctcccgg tagcggatca
    13081 tcgtggcgaa gtgaagcgcg aggtccgggt gaacggggaa ttccgaaaaa ccgggttcgg
    13141 tgagactgcg cgtcagttcg atctcccgct ctccccgaat gttgtagaag atgacggcgt
    13201 cgccgctctt gaggcgtccg atagggtttc cccgttcatc gaccagaacg agaggttcca
    13261 gggtttcatc ttcgcgcccg gcccggtagc cctcgcgtac ggcccgtgag agcgcatcgc
    13321 ccgcccattt cgacatgggt tcattcctcc ttgcggttcg gttcccggaa gtggaacggc
    13381 gacgctccac agcgattccc gccgcccgct ccgcgtgttc tctgtagcgg aatatcccgg
    13441 ggctgtaaag gggaaatccc gacgcgtggc ggccctccgc tgccttcgac attgcataaa
    13501 aggggcgctt tgtggtaagg tatcacgtct gacgcgttta acgggttgag ttgccaaaag
    13561 atgtcgaagc cgaaaagccc ggacgagatc ctgcaggagg agttcctgcg agagcgggcg
    13621 gaggtcctcg gtcgggccgg cacccgcgtc caggaatccc tgaacgccct ccggcggatc
    13681 ggggaagaga tcgcggcggt agagggccgc tatgaggaac tggcgccggt ccaggccgtg
    13741 agtgcggcgg gctgcgggga gagtcaggcg caacgggcgc ggttgcgccg ggagatcgat
    13801 gcgcaaatcc accggtacaa tcgggtccgg gaagaggcga agctgcgcta ctactacctg
    13861 atcgtaaccc gggaggccct cggtttgcgc cgccaccact gggtggagtc ggtataccgc
    13921 attcctccga agaaggccgc gccgcaggac ggatgatgga tatctacacg aaacagagaa
    13981 aacggatggt cgagacccag ctccgttcgc ggggcattcg ggacgaacgg gttctgaagg
    14041 ccctggaggc gattccgcgg cacctgttcg tggacgaggc cctgagggat caggcctatt
    14101 tcgaccatcc gctgcccatc gatgcgggcc agaccatttc acagccctac atcgtcgccc
    14161 tgatgacggc cgcccttgaa ttacgcgggt ccgagcgggt gctggaaatc ggcaccggat
    14221 cgggttacca gacggccgtg ctggcggcgt tggcggatca ggtcttttcc atcgagcggg
    14281 tggctccgct ggccagccgg gcccgcaaga ttctcgacgc cctggacgat tacaacgtcg
    14341 cgatccggat cggcgacggg acgtacggtt ggcgggaaga atccccgttc gatgcgatca
    14401 tcgtgacggc gggcgccccg cgcgttcccc gaattctcgt cgagcagctt gcggtgggcg
    14461 gacgcctggt catcccggtc ggggaccgcc acacgcagga cctcgtccgg gtgatccggc
    14521 aatcggaaga tgccggcgac atcaagcagg aagatctggg cggatgccgg ttcgtgagcc
    14581 tgatcgggga gcacggatgg cagacctgac ccctccgaca ttccggcgac gggaccgatc
    14641 tttggaaaga gggtcggggt gaaacggaca ttcgttcttt tggcgctcct ggcgtgtacg
    14701 ctcgcctgca cgggatatct cgacagaacg gagacgtccc gaggtgtcta tcacctcgtc
    14761 cagcgcggcg aaaccctttc cgtcatcgcc cggacctacc gggtgaccgt ccaggacctg
    14821 gccgagtaca acaacatcac cgatccggac atgatcgtgg agggcagcgc gttgttcatc
    14881 cccggggcgg tccagatcca tgacatcgac cttccggcga ttcgggaatc ctcgccgtcc
    14941 gtccctgagc cggtcgcggc ggttcccccc gcgtcgccgt ccccgcgaac ggtgccccgg
    15001 acccagccgg ttccggacgt gcgcgcaccg tcgtccgggg cggcgaagcc gccgcccgcc
    15061 gcggacgtca gcgggaagac gcgtccggca aaagattccg ggggtgcggc cgtccaggcc
    15121 cgtccttccc ctccgcgaga cgccggaccg ccgacagccc gaccacgccc ggcgccggca
    15181 accccttccg aagatatccg ctttgagcgt cagcgattta tctggcccgt cagggggccc
    15241 gtcaaaacac ggttcggcat ccagccgaac gggctcaatg ccaacggaat cgaaatcgat
    15301 gccccggaga atacgccggt tctggccgcg gccggcggga cggtgatttt ctcggacacc
    15361 cttaaagagt acggagaaac cgtgatcctc aaacatcccg atgacttcaa gacggtttac
    15421 attcagttga agagccgccg ggtccagcgt gaccagcagg tcaagcgggg cgagatcatc
    15481 gccctgacgg gaaagccgga aaagaagcgg gaaccctatt ggaactttga aatccgatac
    15541 aggaacaagg cgcgcaaccc gatgtttttt ctcccctgac tcccgggttt ttccagtgcg
    15601 ccccgttttc gggaaggttc agccgtgaac gagatgtttg aacaaggacc gatccgtccc
    15661 cccagcgagg ccaggagcct cctgatccgg gtgacccgga attgcccctg gaacaagtgt
    15721 gccttctgtc acacctaccg gggaaaagcc ttttcgctgc gcaccgtcgc cgagatcaag
    15781 gaggaaatca cccgaatccg tcagatcgcc gatcaaatcc gccagtggtc ctggcggcag
    15841 ggggacgggg gacgcatcac ggatcgcttg gtcaaccgga tctacagccg ggaggacgac
    15901 gtcgacgacg gctatcgcag cgtggccgcc tggctgtatt tcggcgccac ctcggtcttt
    15961 ctgcaggacg ccaactccct gatcatgaaa acggacgacc tggccgaggt gctgaccttc
    16021 atcaaggccc agtttccgtc cgtcggccgc atcacgacgt acgcccgctc ccatacggcc
    16081 gcccgaaggt ccgtcgagga tttcgtcaaa ctgagggagg ccggtctgac gcgcatccac
    16141 atcggcatgg agagcgggta cgatccgctg ctggccttca tccgcaaggg ggtgacggcg
    16201 gccgaccacg tcaccggggg acagcgcatc gtggccgccg gcatttcgct ttcggaatac
    16261 gtcatgcccg gcttgggcgg ccgccggtgg agccgggagc atgcgctggc gacggccgac
    16321 gcgatcaacc ggatcaatcc cgagttcgcg cggttgcgga ccctccacgt cgtccccggg
    16381 acggatctgc acgacctgat gacgcgcggg gaattccagc cgctgagcga cgaaaagatc
    16441 ctgagggaaa tccgctgttt catcgaagcg ctcaacggca tcgaaacgac gctcgtcagc
    16501 gatcatatcc tgaatcttct ggaggaactg gaggggaaac tcccggagga caaggcgcgt
    16561 ctgctggcaa cggtggatcg ctacttcgcc ctgcccgatg aggatcggcg catctatcgc
    16621 ctggggcggc gccggggggt gtaccggcgt ctcgacgatc tggccgacgc gcgcgtctac
    16681 cggcgcttgc aggacgtcgt cagcgcctat cacgagggcg accccgggcg tttggaccag
    16741 catctggacg ggatcatgaa ccggtacatt taacagggga aaccgcgccc gccgtgattt
    16801 gcgattgacg cggcagtcaa agtgtgataa cctacgaatt tcaaaataaa aggtaggtgt
    16861 aatcggatgt ttaaggtagg ggatttggcg gtctacccgg cgcagggcgt gggggtcatc
    16921 gaggccatcg aacatcgcga attcatgggg agcaaacagc ctttctacat catgaagatc
    16981 atgggaaacg gcatgaaaat catgattccc acgagcagcg ccgaatcggt cggtctgcgg
    17041 caggtcatct cccagaaaga gatccccaag atttacgaaa tccttcgcaa caaggacgcc
    17101 gccatcgaca agcaaacctg gaacaaacgg caccgggagt atctggaaaa gatcaagacc
    17161 gggtccgttt tcgagattgc caaggtcctg cgcgacctgt ttatcctcaa gtccgacaag
    17221 aatctgtcgt tcggcgaacg gaagatgatg gatacggcca agagtctgct gatcaaggag
    17281 atctccgtgg cctgcaacac cgaagagacc aagatcgaac aggatctgaa gaggatcttt
    17341 cccgttcagt aagcccccac tcaaccgcat cgagaggttt ccggacgcac gagagaacgg
    17401 gggaagttgt ttgctcccgt tttccgggta tggcgtccgt tttttgaaat gagcggcggt
    17461 ttcaaccgcc cgcgcgaatt cgagaaagga aggaggtgaa gggagaatga ttattcgatt
    17521 ggtgctgatc ctggcctgtt ccgtcagcgg ttactttatc gtttaccatt atgactgggg
    17581 atttcccaca tccctgctcg gtctggttgc cggtattatc atcgccctca gtgtcatcca
    17641 gatcgaacag gccatccgca aggtctcgat ccgggtgatc ttcggtggcg tcgccggtat
    17701 gttcatcggc ctgcttatcg cctttctttt tgcctacggg ctcagttttg ttaccaatat
    17761 tagtgaaaaa cgtcagatcg ctccctggat ctacgccctg ttgaccgtaa ccatgggcta
    17821 tctggggctt gtgctggggt cgaagaagat cgaagaagtg cacgtgttcg gacttcacgc
    17881 gccgaaggag cccgtcgatt gccggatcct ggatacgagc gtgatcatag acggacgcat
    17941 tgccgacatc tgcgatacgg ggttcatcga cggcaatttt atcgtcccgc gatttgttct
    18001 cgacgagctt cagtacgttg ccgattcctc ggattccctg aagcggtccc gcggccggag
    18061 gggactcgat atcttgaacc gcatgcagcg cgcctcgggc atatcgatcg aagtgctgga
    18121 tcaggattat ccaaggatca agggcgtcga tgccaagctg gtggccctgg ccagggagaa
    18181 aaaggggaag atcattacca acgatttcaa cctgaacaag gtggcggagc tgcaggggat
    18241 caagatcctc aacgtgaacg aattggccaa cgccgtcaaa ccggtcgtcc tgcccggtga
    18301 ggcgatgacg gtcaagatca tccgcgaggg gaaggagccc gggcagggtg tggcctatct
    18361 cgatgacggc acgatgatcg tggtcgacaa cgcccagcga taccaggggc aactcgtcga
    18421 tgtccaggtg accagcgtcc tgcagaccac cgcgggacgg atgatctttt ccgagatgaa
    18481 ggggcttgcc caggacaaga agatgtttgc cctgggggcg aaatcgtgaa acgacgaatg
    18541 gccggaggcg ccctcggcgc cccggcgacg cggcttttat gaacgtgacg gcgatcatag
    18601 cagccggcgg agccggcagg cggatgggaa cggcccggcc caaacagtac cttccgctgg
    18661 gcgacctgcc ggtgatcgtc catgtcctga agacgttttc ccgcgtatcc gcggtgacgg
    18721 agattctcct ggtcgtaccc cgggcggatg tgccgcatgt ccggtcggaa atcgtggggc
    18781 gttacggcct tggcaaggtc cgccgggtaa tcgcgggggg cagggagagg caggactccg
    18841 tcgcaaaagg gcttgccgcg gtccgggcgg agacgacgga catcgtggtg atccatgacg
    18901 ccgtccgtcc gctggcaacg ccggccctgg tcgaggcggc catcgccggg gcggtttgtt
    18961 ggggagccgt cgcgacgggc attcccgtgc gggacaccgt gaaatcaacc gatgccgagg
    19021 ggtgggtgga agagacccta ccccgggaga ggctgtgcct cgtccagacc ccgcaggcct
    19081 tccgtctgga gatccttcgg cgggcccatg aacgggccgc cgccgaagga ttttacggga
    19141 cggacgacgc cgccctggtc gagcggacgg gaacgcgggt gcggatgatt tccggactgg
    19201 cgcggaacat caagatgacg accccggaag acctggactg ggcccgctgg ttcctggaga
    19261 aacggggaag agagatgggg gagggcgcaa cggagatgcc ggagcgtgtc ggctgcgggt
    19321 atgacagcca tcgcctggtg gcggatcgac gactgatcct cggcggggtg gacattcccc
    19381 atccccgggg gctgctcgga cactccgatg gcgacgccct gatccacgcc gtgatcgacg
    19441 ccctgttggg ggccgtcggg ggcggggaca tcgggcgcct tttccccgat tcggacccgg
    19501 cctatcgcgg cgtgtccagc ctgaccctcc tggcgcgggt ctgcagcctt gtgaaggaga
    19561 aaagattcga ggtcgttcat ctcgatgcca cgatcctgat ggagaaaccc aagttggcgg
    19621 tgcacatccc ggccatgatc gaacatcttt cggcaacgct ctgcctggac gccggtcgga
    19681 tcaacatcaa ggccaagacg aacgagggca tggggttcgt cggccgggag gaaggggtgg
    19741 ccgccttcgc cgtcgcgacc gtgcgggagt gcggccgatg aacgaagccg cccagccgcg
    19801 cgtacgtttc gccccatcgc cgaccggaga cctccacatc ggcaacgccc gcaccgccct
    19861 cttcaattgg ctgtttgccc gccataccgg gggcgccttc atcctccgca tcgaggatac
    19921 ggaccgggat cggaccgagg acgtcttcat cgccaacctt ctggaagacc tccggtggct
    19981 gtcgctcgac tgggacgagg gtccggatcg tgtgggggcc tgcggaccct atcgacagag
    20041 cgagcggctg gatctgtacc gggaacatct ggcgcggctt atcgaggccg ggtgggttta
    20101 cccctgctac tgcgcggagg ccgagctgga ggcggaacgg gccgccctcc tggcgcggcg
    20161 gatggcgccc cgttacctgg ggaaatgccg gcagttgacg gtcgccgacc ggcggcggta
    20221 tgaacgggag gggcgccgcc ccgcttaccg gtttcgcgtg gcggaaggtc ccgtatactt
    20281 caccgatctg atccgcgggg agatgtcttt ccagggcgag gccctggggg atttcattgt
    20341 cgtccggtcc aacggcattc ccgcttacaa cttcgcctgc gtcgtggacg atcacctgat
    20401 ggcgatttcc cacgtcatcc ggggcgagga ccacctttcc aacaccgccc tgcaatgttt
    20461 gctatatcgg gccctggagt tcaatccccc ggtcttcgcc catcacgccc tcatcctggc
    20521 tccggaccgg gccaagctgg gaaagcgcca cggcgccgtg tccgtccgca ccttccggga
    20581 gcggggcatt cttccgcagg ccctgtcgaa ttatctggcc attctcggaa gctcgctcac
    20641 ggagaaacag gaaatctgca cgcaggaatc cctggttcgc gatttttccc ttgaccggac
    20701 gggtcggggc ggggccgtct tcgatgaggc gaagctggcc tggctcaatg cgctttatct
    20761 gaaacggatg ccgcttgacg atctcaccgc gcggatcctg cccttcctcg agcgggaagg
    20821 gatgaacgtc cggaggctgg atcgacgatg gctgatggcc ctggtggagg ccgtccggga
    20881 caattgcgcg acgctggcgg acgtcccccg tcacctgaat gtttttttcg acgagcgaca
    20941 cacctggaac gacgcggccc gcacggttct gacggacgac aacgcccgcc gggtcgtccg
    21001 gctgttccgc gacctgctcc tggccgatct tccgcccgac gagcaggtct ttgcgcgctc
    21061 gctcaaggcg ctgcgaaaac aggccggggt caaggggcgg gcgctctatc ttcctctgcg
    21121 ggcggccgtg acaggggcga cggaagggcc ggaactggat cggatcatgg ccgcgctggg
    21181 ctgggaagcg gtggtgcagc ggttggaccg cgtcctccgg gagtcctttt gaagggtcct
    21241 caattggatc ggggaccgac ggggtgttga ccgctcaaaa gacgtgatcc cccgcaggcc
    21301 cacggggatg ccgtaccggt cacccggagc ccttccgttc cgtcttcctg tctccaaccg
    21361 gtcgggtacg atccgccggg aacccggaca caggggcagg ttcccggcgg atgtctatcc
    21421 gaggtaggtt aaaatcactt ctttgccttg gccggcttct tagcgaccgc ttttgccttg
    21481 gccggcgcct tggctttggc ctttgccttt gccggggcct tagcgaccgc ctttgccttg
    21541 gccggcgcct tggcttttgc ctttgccggg gccttagcga ccgctttcgc cttgaccgat
    21601 gctttggctt tggccggagc ctttgcgact gcttttgcct tggctttggt tggtttcgct
    21661 gcggctttca ccggcgcctt cgcagcagcc tttgctttgg caggggcctt taccgcggcc
    21721 ttcgccggtt ttgcctgaac cttctttgct gtcgccattc gtccttcctc ctttttaaat
    21781 gttcgatccc gcctcgtggg ggtctggatg gaccgtgacc tcccgcgcgc ggaatcgtga
    21841 gacgcttcgg gatgaatact ttttctcgtc gctttcaggc cagcggcaac gcctgaaatc
    21901 ttggtgccag cagtggaaag gtttttcttt ttttgttttt gagccagggg agattggagc
    21961 ttctgttttt cttcgacggg acctgttcgc ttcggtttga tgaccctccg gcgagcggcc
    22021 ttttctgccg cgtgaatttg ctggggagat aaaatctcca cgcgcaagcc ggtaaacgga
    22081 cagatcgagc cgggtgttaa atggtatttt tgagcgatgg aatcttcaat ctggtaaccg
    22141 tcgacaagct ccttattgcc taagacatcg aggaataa
//
LOCUS       DAOOLJ010000027        21915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2308_length_21915_cov_18.768801, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000027 DAOOLJ010000000
VERSION     DAOOLJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 21915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21915
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_21915_cov_18.768801"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..237
                     /gene="rrf"
                     /locus_tag="PLW16_06125"
     rRNA            122..237
                     /gene="rrf"
                     /locus_tag="PLW16_06125"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            392..1141
                     /locus_tag="PLW16_06130"
     CDS             392..1141
                     /locus_tag="PLW16_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB70516.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGKIFTKIIKEITLAARLGGGDPD
                     GNARLRTAILAAKDENMPKDNIERAIKKGVGEIGGASSFEETTYEGYGPGGVALLVEV
                     LTDNKNRTVAEIRHILSKHGGNLGENGCVAWMFDKKGSLLVNKSDIDEDALMELALEA
                     GADDVREEEHEFEVITSPGAFETVRRAIEQRGIKLLSASIGMIPQNTVQLEAARAEQM
                     IKLMEKLEDNDDVQNVYANFDIPDEIMEKLS"
     gene            1163..1642
                     /gene="ruvC"
                     /locus_tag="PLW16_06135"
     CDS             1163..1642
                     /gene="ruvC"
                     /locus_tag="PLW16_06135"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPB70517.1"
                     /translation="MRVLGIDPGSHVTGYGLVDHEKGGLKVVTYGEIKPARGARLTAC
                     LAIIHRRLQEIVAEHRPDALAVEDIFYGKNVKSLIRQGHVRGVTLLTGALAGVAVYAY
                     SPLEVKQAVVGYGRAEKSQVQRMVMAILGLADVPPEDAADALAVAICHAHFRKEPPA"
     gene            1643..2248
                     /gene="ruvA"
                     /locus_tag="PLW16_06140"
     CDS             1643..2248
                     /gene="ruvA"
                     /locus_tag="PLW16_06140"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPB70518.1"
                     /translation="MIARLSGTLIHKNVNFIVIDAQGVGYRVFVPLTTFYELPEPGER
                     IALHIHTQVREDAISLYGFHAEREREIFQIMLGVSGIGPRLALNILSGISAAEFVAAI
                     LQGNLGRLVGIPGVGKKMGERLIVELRDKIARLEAVAVVAGERRVISLEFLKEDALSA
                     LTNLGYKPAAARGAVDAVIAAAAEPPSLDVLLKEALKRLAG"
     gene            2309..3334
                     /gene="ruvB"
                     /locus_tag="PLW16_06145"
     CDS             2309..3334
                     /gene="ruvB"
                     /locus_tag="PLW16_06145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPB70519.1"
                     /translation="MSDKNPVIEPEAAEAEGLFETSLRPRSLAEYIGQEKIKSNLAVF
                     IEAARRRREALDHVLLYGPPGLGKTTLAYIVARELEVDVKVTSGPVIERPGDLAAILT
                     NLHEHDILFIDEIHRLSHVVEEILYPAMEDFHIDILIGQGPSARSMKLEIPHFTLIGA
                     TTRAGLLTSPLRDRFGMAFRFEYYTPEELAVIIKRSAGILDIGIDRAGAEEIACRSRG
                     TPRIANRLLKRVRDFAEVRADGVVNRRVAVDALKLLDVDPQGFDHMDRTILLTLIDKF
                     DGGPVGIDNLSAAIGEEKTTLEDVYEPYLIQEGYLQRTARGRVATRIAYEHFGRTWLG
                     QPQSDLF"
     gene            3336..3908
                     /locus_tag="PLW16_06150"
     CDS             3336..3908
                     /locus_tag="PLW16_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPB70520.1"
                     /translation="MKVLLHTCCGPCTIYPLRILRETGCEPIGYFFNPNIHPSLEYRR
                     RAETLAAYAQMQALPMLWAEEYAMETFFRAVAGREADRCRHCYTLRLAEAVRMAKARG
                     CEGFTTTLLYSKYQKHDLIRELGDRMGQENGIPFVYHDFREGWQEGVRVSREIGMYRQ
                     PYCGCLYSEKERYGRRSVTPRNDASAGAPA"
     gene            4058..5053
                     /gene="lpxC"
                     /locus_tag="PLW16_06155"
     CDS             4058..5053
                     /gene="lpxC"
                     /locus_tag="PLW16_06155"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPB70521.1"
                     /translation="MVRKLLIFTLKVYRDVMSKMFLQRTIQREIHCRNIGLHSGRKVA
                     MTIKPADADAGITFVRNDVAGRPEIRARYDGVSDTTLATTIGCNGTRISTIEHLMSAL
                     FGMGVDNAVVEVDAPELPIMDGSALPFVDLLKSVGTRAQGRCKKILVVEKPVSVEDGY
                     GSATFLPAPEFRITCEIDFDHPVIGHQTFSLTFSDDLYEKEICAARTFGFLKDVEYLQ
                     ASGLALGGSLKNAVVMDETRILNKEGLRCPDEFVKHKILDAIGDLSLLGMPVIGHFVA
                     RKSGHRLNNLLLRELMNRRDCWRIVTALGRQDTATERTGLKPPAFSILDAVAVRA"
     gene            5259..5828
                     /locus_tag="PLW16_06160"
     CDS             5259..5828
                     /locus_tag="PLW16_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HPB70522.1"
                     /translation="MVRKCLLSLCGVILLLSVGPSGVLAQEARLTDIRGFTDSKQVLV
                     YAKVTNCFTPKMEQAIYAGIPTTFTFFLNLYQARSWCLDRRLAFVEVRHTLKYDRVRK
                     LFFVSVGDGGEPVGFQDLGTAKLAMAELNGVALFPVSKLERGRTYYLRLKAKLEEVRL
                     PMRLEYILFFASLWDFETDWYRYRLSMTP"
     gene            5825..8026
                     /locus_tag="PLW16_06165"
     CDS             5825..8026
                     /locus_tag="PLW16_06165"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB70523.1"
                     /translation="MKKARPPLDISEARRRRRERIIIVITMAVIAGLTYLESQLSHQQ
                     GVLPASNNILIFGLININIILIILLIFLIVRNVVKLVFERRSGVIGSRLRTKLVAAFV
                     GLSLIPTIILFLVAINFLSYSIDTWFSIRFGDTLNKTEEVAQVYYQQAADNAKYFARQ
                     ISADITTHRLHERDRMTYLRQLVEQRQRSSRLGMVEVYFENERENVVFQDPEHLDQEP
                     ILLSPKLREEVFAGREVSREQAGAGGDMISGVVPVFAALGPHEVIGIVVVSRFIPGTI
                     VEKIKAISRSSEQYRQLTLLKNPIKFSYIVTLFIVTLLIIFSATWFGMFLAKGITVPI
                     QDLAEATKRIAQGDLEHQIEIEADDEIGVLVDSFNQMTRDLRKSNMDLEQRRRYMEAV
                     LRHVSAGVISIDASGVITTINRAVEKMLGIRAETILHRPYEEVLAREHLEMVNAYLQE
                     MRSKRNGFLEKQVELTIGERAMTLLMTTNVIHDDDGRDLGMVVVFEDLTQLQKAERAA
                     AWREVARRMAHEIKNPLTPVQLSAQRLQKKYGEKLGGDREVFFECTKTIIDQVEVLKN
                     LVNEFSRYARIPVTNPAPHDLNEVIADSVVLFQDAHKEIRFTFETEDALPIVNIDPEQ
                     IKRVMVNLLDNAVAALREHDGCIAIRTFYDPAAQKARVEVADNGTGVPPSYKLKIFEP
                     YFSTKGAGSGLGLAIVNSIIADHHGKVSVRDNVPRGTVVAFEIPVREGVAV"
     gene            8076..9440
                     /locus_tag="PLW16_06170"
     CDS             8076..9440
                     /locus_tag="PLW16_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB70524.1"
                     /translation="MRKTILIVDDEESIRQSLGGVLNDEGYEVLAAASGEEALRMIDD
                     EIPSLVLLDIWLPGMDGIEVLTRIRSEYPQIRVIIMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKVILLVSHALDMVNLEEENILLRQKLGREYELTGESPPISELKETIGIVAHT
                     NAWILIMGENGTGKELVARSVHRQSRRANKPFIEVNCAAIPEDLIESELFGHEKGAFT
                     GATAKKRGKFDLAHEGTIFLDEVADMSLKAQAKVLRILQEKKFERVGGTRLIPTDVRV
                     LAATNKDLEQEMEAGRFRQDLYYRLNVIPLRVPPLRERREDIPLLVNRFLQDLAQKEG
                     EEQKTMTMAAIGVLMEHPWPGNVRELKNIVERLVIMTPGNVIDARDIPSLNKDAEVVL
                     DSSFYDSDSFKAAKVEFEKSYIVKKLREFEGNISKTAEAIGLERSNLHKKIKAYGLDV
                     KDAG"
     gene            complement(9492..10460)
                     /locus_tag="PLW16_06175"
     CDS             complement(9492..10460)
                     /locus_tag="PLW16_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB70525.1"
                     /translation="MKKNIAAGILVSLALVYLSVRGIPWAGVREGVAAMHWGFALPAL
                     AAMLLMQVFRSIRWGVILGPMEQIRQRTLFPITSVGFLAIVSLPARLGELARPYLIAQ
                     RSGIRMSAALGTIFVERVFDGLTVLSLFALTPLLTPLPIWLLRSGAIFFALTVGLMAA
                     MALLLLKRERALGLLRPLLAKLPAPYGAELAGLVDRFVDGFQMIGDGKRFLTVAALSL
                     VIWVLDVFAIYILFPAFGLALPVGAALVVMIILVIGIALPAAPGFIGNWHFACILALG
                     LFGVAKTDALTFAILYHVLSLGVVIALGLPFLPFQRFSLADVRHQL"
     gene            complement(10582..10980)
                     /locus_tag="PLW16_06180"
     CDS             complement(10582..10980)
                     /locus_tag="PLW16_06180"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPB70526.1"
                     /translation="MKRKSLLSFLMMSLMLLGGCAAMAVGVTAVGVGSGTYLYINGDL
                     NTDYYTSFDRVWAACEKTIADMRGMEVKPDKDIGKGTISTVINDEKVQFTVKYKAKDL
                     ITVGVRVGLLGDKLSSQLIHDKIADNIANN"
     gene            complement(11082..12143)
                     /gene="dnaJ"
                     /locus_tag="PLW16_06185"
     CDS             complement(11082..12143)
                     /gene="dnaJ"
                     /locus_tag="PLW16_06185"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPB70527.1"
                     /translation="MKRCYYEVLEIERGASDEEIKKSYRRLAMQYHPDRNPGDHEAED
                     RFKEAAEAYEVLSDPQKRSIYDQYGHEGLSGAGYHGFSGFEDIFSSFGSIFEDVFGFG
                     GGRARGRTGPRSGADLRYDLKISFKEAATGTTRDIDVEKYERCEGCHGSGAAPGTSPE
                     VCRYCHGRGQVTQSSGFFSVRSTCPQCRGQGSVITQPCPQCRGTGRTRITKTVQLKIP
                     AGVETGSRLRLRGEGESGDPGGPAGDLYVFIYVDPHEFFERNDDDLYCRVQISFVQAA
                     LGGTVEVPTLNGNEKLKIPRGTQSGKTFRLKGKGIPHLRGFGRGDQIIETVVAIPTNL
                     TRKQEELLREFARLGGAPG"
     gene            complement(12147..12533)
                     /locus_tag="PLW16_06190"
     CDS             complement(12147..12533)
                     /locus_tag="PLW16_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70528.1"
                     /translation="MKRPDIPDYRKKQALLYIQKSVADLTVYGNGFLAAGRMADALEF
                     FEAAADREGLEKIRTWAESEGDAMIYLNCAKALREEPTPDGWVRICRTALAKGKLRFA
                     REAAVRSGDPALLEDVEHNLREQAGG"
     gene            complement(12622..13515)
                     /locus_tag="PLW16_06195"
     CDS             complement(12622..13515)
                     /locus_tag="PLW16_06195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB70529.1"
                     /translation="MNEPTGLNKTDLAGLKLISRGKVRDIYDLDPCLLIVATDRISAF
                     DVIMDEPIPGKGEILTRMSAFWFGAMADLIGNHLISTDPDAYPESCRPYRDILKGRSM
                     LVRKARPLPVECIVRGYLSGSGWKDYRRDRTVSGITLPAGLKESARLPKPLFTPSTKA
                     PEGEHDATITRGDMEALIGRDLAEQVLRTSLAVYRRAGEIAERAGIIIADTKMEFGIC
                     DGDLILIDELLTPDSSRFWPRSDYAEGRVQKSFDKQFLRDYLLSIPWNQTAPPPPLPR
                     DIIEKTKEKYEEALRRLVPDR"
     gene            complement(13557..14186)
                     /locus_tag="PLW16_06200"
     CDS             complement(13557..14186)
                     /locus_tag="PLW16_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB70530.1"
                     /translation="MYLEQVQVSAMAVFAYIVGDPDTGEALVIDPAANVDGLIRIAQS
                     RKLTIKTIVNTHGHVDHISGNLEMKQKTGAEIIIHEADAPMLVDTPPMLLRMFGAAKS
                     PPADRTVREGDVISVGRVALAVLHTPGHSPGGMCLYRDGHVFTGDTLFVEAVGRSDLP
                     GGSWPLMLRSIQEKLLTLPDETLVLPGHNYGSMPTSTIGHEKKYNPFLN"
     gene            complement(14256..15602)
                     /gene="rsmB"
                     /locus_tag="PLW16_06205"
     CDS             complement(14256..15602)
                     /gene="rsmB"
                     /locus_tag="PLW16_06205"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPB70531.1"
                     /translation="MSETARSVAVDLLTRVERTGAYAEPLLNAALSGDSLGDPRDRGL
                     ATQLVYGTLRVQGRLDWILRQLYRGRLADLEPVTRAILRTALYQLEFTDRIPAFAAVD
                     AAVELAKGRVPDRASLVNAVLRAAVRKRDAIRWPDAVQDPVGHIAATASHPLWLVRRW
                     LAVYGPGETAALCRADNETPPCTLRVNRIRLTPAAAREALAAAGLACEPTFFSPDGLA
                     VAAGAGALGETAPFREGGLHIQDEASQLVAPLVDPRPGETLLDLCAGSGGKTTHLAAL
                     LQNSGEILAVDIHPRKLADLETLARHLGATPIRTWARNAEADLGEAFHARFDRVLVDA
                     PCSGTGTLRRNPEIKWRLQPRDLGTLVRRQRRILDAAAAYPRRGGVLVYATCALLPEE
                     NEDVIADFLERHPEYAPDSPPPPFPAALLDEEGYFRTLPHRDGMDGFFGARLRRLG"
     gene            complement(15637..16422)
                     /locus_tag="PLW16_06210"
     CDS             complement(15637..16422)
                     /locus_tag="PLW16_06210"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPB70532.1"
                     /translation="MKKKRFVDLSIALETGLPSDPPMMIPRVDYIDHAMGTASMLDFF
                     PGLKADQLPGGVGWAVEFLTLSTHSGTHLDAPYHYHPKMDRGRPAATIDEIPLAWCLS
                     DGVLLDFRHKADGERITVADVKRELDRIRYELKPLDIVLVQTGADQAWGKPEYLVKGA
                     GMDRESTLYLTEQGVRVVGIDAWSWDRPLPFLAAEFKEKGDPRVIWEAHFAGIETGYC
                     HMEKLANLSAIGRPHGFTVSCFPIKIKRASAGWTRPVAIVEED"
     gene            complement(16472..17407)
                     /gene="fmt"
                     /locus_tag="PLW16_06215"
     CDS             complement(16472..17407)
                     /gene="fmt"
                     /locus_tag="PLW16_06215"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB70533.1"
                     /translation="MQTSVMFMGTPEFALPSLERLAVNGWPIAAVVTQPDRPRGRGRS
                     RIAPPVKDAALRLGLPVLQPERVRDPAFLDTCRALAPDVIVVAAFGQILPAVLLEIPR
                     FGCINVHPSLLPKYRGAAPMNWTLIRGESVTGVTIMRMNEAMDAGDILIQEETPIDPG
                     EDYGALHDRLAILGAGLLARALDGIVSGTLTGTPQNPSGATFAPKIGREDGLIRWDAP
                     ADAVVNLIRGLSPTPGAWTRCDGKILKVFRAEASPGPVADAPGTVGEAMPGGLPVAVR
                     GGRVCLTEVQLENKKRLPIGDFLRGQRLASGSRLG"
     gene            complement(17436..19667)
                     /gene="priA"
                     /locus_tag="PLW16_06220"
     CDS             complement(17436..19667)
                     /gene="priA"
                     /locus_tag="PLW16_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPB70534.1"
                     /translation="MFIQVAIDIPSERTFTYRVPSFLAAEVAPGKRVRVPFGRRRQVG
                     VILAVDDVAPDAGGKEIREILDILDPEPLFGPEELRFYRWAASYYLYPLGKALADVLP
                     GTEYRSGGQRIRLTEDAADRITALLSPAADVLCAVLAGRRNGLTLNRLRKVTGIEDIR
                     KEIRELQDAGLVRVTEAERRLAPRRGGEAPGENVRTTEAVPVLNPDQRNAVETIRESL
                     NRGGFAPFLLHGVTGSGKTEVYLRAIADVLERGGGALLLVPEIALTGQLLNRLQARFP
                     RQAVAVLHSGVSRAARYDQWRQIRQGRLRLIIGARSALFAPVRDLKLVVVDEEHDLSY
                     KQDDRMHYQARDLAVIRAKLAGAAVILGSATPELQSYFNIAARKYRLLTLPDRVLSKP
                     LPQVEVIDMRGRREAAGQPAVLSAPLQTALRETLSLGRQAILFLNRRGSHAFMVCLDC
                     GQAFRCLNCAVSLTYYGGDAILKCHYCGFRHPVPPTCPACGSSRLGGFGVGTEKVEAE
                     VRATFPEARIARLDSDTTTRRGAHIEILQRLAGQEIDILIGTQMVAKGHDFPGVTLVG
                     VISADTSMNLPDFRAAERTFQILTQVAGRGGRGETPGRVLIQTFNPGHYALLKARHHD
                     YTGFYQEEIASREALSYPPFSRLVSLHLSCRSREKGRAAGETLGRLARELATAGRSAP
                     GIEVIGPAEAPVAKIKGRYRWQLLLKGKHVTSLHALARSLSEHAAHTGLDVRIDVDPV
                     HFL"
     gene            complement(19813..20241)
                     /gene="dksA"
                     /locus_tag="PLW16_06225"
     CDS             complement(19813..20241)
                     /gene="dksA"
                     /locus_tag="PLW16_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HPB70535.1"
                     /translation="MRLPPEKTFDALSATEGYVMAPMKPEKLEFFRYMLKQKINELLE
                     DAEKTVSEMTSGKENYPDPNDRASLESDRNFELRIRDRERKLIAKMKEAIKRIDDGSF
                     GNCEVCGGPISEKRLVARPVTTLCIDCKTKQEKLEKLKGE"
     gene            20346..21185
                     /locus_tag="PLW16_06230"
     CDS             20346..21185
                     /locus_tag="PLW16_06230"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HPB70536.1"
                     /translation="MQRPEIRNVVSPRRRTAANGAAGSREDVSVVLTAIAEGFLGVPY
                     LAHPLDQTGPEHLVVNLDAFDCVTFVETAVALARLPADGRADREGLARAVEEIRYRGG
                     RLDGYASRLHYFSDWLRDNKARGILEDMTARLGGTPWEKRLNFMTAHRDRYPPLADPE
                     CYRRVRAVEEALTAMPKVHLPRGRFRSQEARIHDGDILAVTALQDGIDVLHVGFAVRR
                     RGRIHLLHASEAAGRVVVSPETVYRYLHRRRARTGLLVARLRAGAGLPTSGRPAGPAG
                     TPT"
     gene            complement(21142..>21915)
                     /locus_tag="PLW16_06235"
     CDS             complement(21142..>21915)
                     /locus_tag="PLW16_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB70537.1"
                     /translation="TSVNLERSFMKRVIEDRKTIIVQNVESDPRTLKPNDPKYGPPSF
                     LSMPIFADHALIAVLNLANKESGLLFDELDERIVSTMLGSVSFALENARLHSQIKAQL
                     EEILAKNVTLEREVRDRQRVEEDLRRVNVELKDANDRMTAAYDWMRANRDRLLNHRDR
                     EEIGFVVDREGTVEEITERALEYAGKPRGEMIGASLTDLLAAPGRDAFKDELRHAWSG
                     VTRTLRVEMLQPLESETTCDATLTRLTSASRRALLVVLR"
BASE COUNT         3894 a         6762 c         7297 g         3962 t
ORIGIN      
        1 acagtaatgt gttgagctta ctgatactaa tcggtcgtga ggcttgacca taattttgtc
       61 atcttttgtt cccgttttgc cccaccatgc catatgcaat tgttgctctt ttagaaatca
      121 tttcggtggc tatagcggag aggccccacc cgttcccatc ccgaacacgg aagttaagct
      181 ctctggcgcc gatggtactg catgggtgac tgtgtgggag agtaggagct gccgagatat
      241 ttatcgaacc ccttcaagcg attgaagggg ttttttttgg tcccgaagat ttccgttttg
      301 caaagtcccc gtcttgtgat atgagggaag cgcgcgtaat cttgccgtgt tccgggctca
      361 gcggacgggc aaaacaagga gagaggacag catgtccggt cattcgaaat ggagcacgat
      421 caagagaaaa aagggcgccg cggatgcgaa gcgcggcaag atattcacga agatcatcaa
      481 ggagatcacc ctggcggccc ggctgggcgg cggggacccc gacggcaacg cccgcctgcg
      541 gacggcgatc ctcgcggcca aggacgaaaa catgcccaag gacaacatcg agcgggcgat
      601 caagaagggc gtcggcgaga tcggcggggc gtcgagtttt gaggagacaa cctacgaagg
      661 atacggtccg ggcggggtgg ccctgctcgt cgaggttctc acggacaaca agaaccgaac
      721 ggtggccgag atccggcaca tcctgtccaa gcacggcggc aacctgggcg aaaacggctg
      781 cgtggcctgg atgttcgaca aaaagggaag tctgctcgtc aacaagagtg atatcgacga
      841 ggatgccctg atggagctgg cactcgaggc gggggccgac gatgtgcgcg aggaggagca
      901 cgagttcgag gtcatcacga gcccgggggc cttcgaaacc gtccggcgcg ccatcgagca
      961 gcgggggatc aaactcctgt cggcgagcat cggcatgatt ccacagaaca cggtccagct
     1021 cgaagccgcc agggccgagc agatgatcaa actgatggaa aagctggaag acaacgacga
     1081 tgtgcagaat gtctatgcca acttcgacat ccccgatgag atcatggaga aactgagtta
     1141 gcggatccgg agaggaatcg tcttgcgcgt cctgggcatt gatcccggaa gccatgtcac
     1201 cggctatggc cttgtcgatc acgaaaaagg cggactgaag gtcgtcacgt acggggagat
     1261 caaacccgcc cgcggggctc ggctgacggc gtgcctcgcg atcatccatc gccgtctgca
     1321 ggaaatcgtc gccgaacatc gtccggatgc cctggccgtc gaagacatct tttacggcaa
     1381 gaacgtcaag agcctcatcc ggcaggggca tgtccggggg gtgaccctgc tgacgggcgc
     1441 cttggccggt gtcgccgttt atgcgtattc gcccctggag gtgaagcagg ccgtggtggg
     1501 gtacggacgc gcggagaaat cgcaggtgca gcggatggtg atggcgatct tgggcctggc
     1561 cgatgtccct cccgaggacg ccgccgatgc cttggccgtc gccatctgcc atgcccactt
     1621 cagaaaggaa ccccccgcct gaatgatcgc ccggctgagc ggcacgctga ttcacaagaa
     1681 cgtcaacttc atcgtgatcg acgcccaagg tgtcggttac cgcgtctttg tgccgctgac
     1741 gaccttctac gagcttcccg agccggggga gcggatcgcc ctgcacatcc acacccaggt
     1801 gcgggaggac gccatcagcc tgtacgggtt ccatgcggag cgtgagcgtg aaatcttcca
     1861 gatcatgctg ggcgtctccg ggatcggtcc gcgcctggcc ctgaacatcc tctccgggat
     1921 ttccgccgcg gagtttgtcg cggccatcct gcagggcaat ctgggccgtc tcgtcggcat
     1981 ccccggggtg gggaaaaaaa tgggcgaacg gctgatcgtg gaactccggg acaagatcgc
     2041 ccgtctcgaa gcggtggcgg tcgtcgccgg ggaacgccgg gtcatctcac tggagttcct
     2101 gaaggaagac gccctgtccg ccctgaccaa tctggggtac aagccggcgg ccgcccgggg
     2161 ggctgtggat gcggtcattg cggcggcagc cgaaccgcct tcgctggatg tcctgctcaa
     2221 ggaggccctg aagcggctgg ccggatgacg tcgggccagc tcgaaagggt gcgggagcga
     2281 aggagaaagg gaaagggagg ggcacgcgat gtcggacaag aacccggtga tcgagccgga
     2341 ggcggcggag gccgaagggc tgttcgagac ctccctgcgc ccccgctccc ttgcggaata
     2401 catcggccag gaaaagatca agtcgaatct ggccgtcttc atcgaggcgg cccgccgccg
     2461 ccgcgaggcc ctggatcacg tgcttctcta cggaccgccg ggtctgggga agacgacgct
     2521 cgcctacatc gtggcgcggg aactggaggt cgacgtcaag gtgacgtcgg gcccggtcat
     2581 cgagcgtccg ggagacctgg cggcgatcct gacgaacctg cacgagcacg acatcctctt
     2641 catcgacgag attcaccggc tttcgcatgt agtggaagag atcctctacc cggccatgga
     2701 ggatttccat atcgacatcc tcatcggcca ggggccgtcc gcccggtcga tgaagctgga
     2761 gatcccgcac ttcacgctga tcggcgccac gacgcgggcg ggactcctga cctcgccgct
     2821 cagggaccgt ttcgggatgg ccttccggtt tgaatattac accccggagg aactggccgt
     2881 catcatcaag cggtccgccg gcatccttga catcggcatc gaccgggccg gggcggagga
     2941 gatcgcctgc cgctcccggg gaacgccgcg catcgcgaat cgcctgctca agcgggtccg
     3001 cgattttgcc gaagtccggg ccgacggcgt tgtgaaccgc cgggtggcgg tcgacgccct
     3061 gaaacttctc gacgtggacc cccaggggtt cgaccacatg gaccggacga tcctgctgac
     3121 cttgatcgac aagttcgacg gggggccggt cgggatcgac aacctatccg ccgccatcgg
     3181 tgaagagaag accacgctcg aggacgttta cgaaccctac ctgatccagg agggctacct
     3241 ccagcgcacg gcgcgggggc gtgtcgccac gcgcatcgct tacgagcact tcggccggac
     3301 ctggctgggt cagccccagt cggacctttt ctgaaatgaa agtattgctc cacacgtgct
     3361 gcggcccctg tacgatctat cccctgcgga tcctgagaga gacggggtgc gaaccgatcg
     3421 gttatttctt caaccccaac atccatccct ctctggaata tcgccgacgc gccgagacgc
     3481 tggcggccta cgctcagatg caggccctcc cgatgctgtg ggcggaggag tacgccatgg
     3541 agacgttctt ccgggcggtt gccggtcggg aagccgaccg ctgccggcac tgctataccc
     3601 tccggttggc ggaggcggtg cgtatggcga aagcccgggg atgcgaggga ttcacgacga
     3661 cgcttctgta cagcaaatac cagaagcatg acctgatccg ggaattgggc gaccgcatgg
     3721 gacaggagaa cgggattccg tttgtctatc atgattttcg tgaaggctgg caggaagggg
     3781 tccgcgtctc cagagaaatc gggatgtacc gccagcctta ctgcggctgt ctttacagcg
     3841 agaaggagcg ctacgggcgc cgttccgtca cgccaagaaa cgacgcttcc gccggagcgc
     3901 cggcgtgacg gaagggaaca gcgcccgccg ggattgtgtc tttcccgcca caccgccccg
     3961 cgggaggggc ttttccgctt ccgcgaccgg ggctccaaac gggattattt ccgtcgggtt
     4021 agattccgtg ccggattaaa tgccgaagca ggggtgcatg gtacgcaagt tgcttatttt
     4081 tacactaaaa gtgtatcggg atgtgatgtc gaagatgttt ttacagcgta cgattcaacg
     4141 cgagatccac tgccggaata tcggcctcca ttccggccgg aaggtcgcca tgaccatcaa
     4201 gccggccgac gccgacgcgg gcatcacctt cgttcgcaac gatgtcgcgg gccgtcctga
     4261 aatcagggcc cgttacgacg gcgtttccga cacgaccctg gccacgacca tcggctgcaa
     4321 cggcacgcgc atctcgacca tcgaacacct gatgtccgcc ctgttcggca tgggagtgga
     4381 caatgccgtc gtggaagtgg atgcgccgga actccccatc atggacggga gcgccctgcc
     4441 tttcgtggac ctcctgaaga gcgtcggaac gcgtgcccag ggccgctgca agaagatcct
     4501 cgtcgtggag aagcccgtct cggtcgagga cggctacggg agcgccacct tcctgcctgc
     4561 cccggaattc aggatcacct gcgagatcga cttcgaccat ccggtgatcg ggcaccagac
     4621 attttcactg accttttccg acgatttgta cgaaaaggag atctgcgccg caaggacgtt
     4681 cggtttcctc aaggatgtgg aatacctcca ggcgagcggg ctcgcgctgg gcgggtctct
     4741 caagaacgcg gtggtcatgg acgaaacccg catcctcaac aaggagggcc tccgctgccc
     4801 cgacgagttc gtcaagcata agatcctgga cgccatcggc gacctgtccc tgctcggcat
     4861 gcccgtcatc ggtcattttg tcgccaggaa atccggacac cggctcaaca acctgctcct
     4921 cagagaattg atgaaccgcc gggattgctg gcggatcgtc accgccctgg ggcggcagga
     4981 caccgccacc gagcggacgg ggctgaagcc accggccttc agtatcctcg acgccgttgc
     5041 cgttcgtgcc tgaagcgtcc ggacgtcttc cccgtttccg tcctccgtcc gttcgccccc
     5101 gatccataaa taccacttgc tttttcttcg gcagtttcct agtataatcc tcggtatttt
     5161 gtaaaagaaa aacaaagcat tataaggcgt tatgcctctc cgtcggcagg tcggcctcac
     5221 gggggaaacg gggccggccc ggggaaagga gtccgctcgt ggttcgcaag tgtctgcttt
     5281 ccttatgcgg cgtcatcctg ctgttatcgg tgggaccgtc gggcgtcctg gcacaggagg
     5341 cgcgcctcac ggacatccgg gggtttaccg acagcaaaca ggtgctggtc tatgccaagg
     5401 tgaccaactg cttcaccccg aagatggagc aggcgatcta tgccggcatc ccgaccacgt
     5461 tcaccttctt cctgaacctt taccaggcac gatcctggtg cctggaccgg aggctggcct
     5521 ttgtggaggt ccgccatacc ctgaagtacg accgggtgag aaaactgttt ttcgtgtccg
     5581 tcggcgatgg tggcgagccc gtcggttttc aggatctggg gacggccaag ctggccatgg
     5641 cggagctcaa cggcgtcgcc ctgtttcccg tttcgaagct tgaacggggc cggacgtatt
     5701 acctcaggct gaaggcgaag ttggaggagg tcaggttgcc catgcgcctg gaatacatcc
     5761 tgtttttcgc atccctctgg gactttgaaa ccgactggta tcggtaccgg ctctccatga
     5821 ccccatgaaa aaggcccgcc ccccgctcga catttcggaa gccagacgcc gccgccgtga
     5881 gcgcatcatc atcgtcatca ccatggcggt gatcgccgga ctgacctatc tggaaagcca
     5941 actgtcccat cagcaggggg tgctgcccgc ctccaacaac atcctgatct tcggtctgat
     6001 caacatcaac atcatcctga tcatcctgct gatttttctg atcgtgcgca acgtcgtcaa
     6061 gctcgtattc gagcggcgca gcggcgtcat cggttcccgg ttgcgcacga agctggtggc
     6121 ggcctttgtg gggctgtccc tcatccccac catcatcctt ttcctggtgg cgatcaattt
     6181 tctgtcctac agcatcgaca cctggttcag catccgtttc ggcgacacgc tgaacaagac
     6241 cgaggaggtc gcgcaggtct attaccagca ggccgcggac aacgccaagt atttcgcccg
     6301 ccagatcagc gccgacatca ccacgcaccg cctgcacgag cgggaccgga tgacctacct
     6361 ccgccagttg gtcgagcagc ggcagcgctc cagccggctg ggcatggtgg aggtctactt
     6421 cgagaacgag cgggagaatg tcgtgttcca ggatcccgaa catctcgatc aggagccgat
     6481 tctcctgtcg ccgaagctgc gagaggaggt ctttgcgggc cgcgaggtgt cgcgcgaaca
     6541 ggccggagcc ggcggcgaca tgatcagcgg cgtcgtcccg gtattcgcgg ccctcggtcc
     6601 ccacgaggtg atcggcatcg tggtggtgag tcgcttcatc cccgggacga tcgtcgaaaa
     6661 gatcaaggcg atctcccgat cgtcggagca gtaccggcaa ctgacgctcc tgaaaaatcc
     6721 catcaagttc agctatatcg tgacgctttt catcgtgacc ctgctgatca tcttttccgc
     6781 cacctggttc gggatgtttc tcgcgaaggg catcaccgtt cccatccagg atctggccga
     6841 ggccacgaag cggatcgccc agggggatct ggagcaccag atcgagattg aggccgatga
     6901 cgagatcggt gttctggtcg attctttcaa ccagatgacg cgggatctcc ggaagagcaa
     6961 tatggaccta gaacagcgcc gccgttatat ggaggccgtc ttgcggcatg tttctgcggg
     7021 tgtcatttcc atcgacgcca gcggggtgat cacgacgatc aaccgggcgg tggaaaaaat
     7081 gctgggcatc cgcgccgaga cgatcctcca tcgtccttac gaggaggtac tggcgcggga
     7141 gcatctggag atggtgaacg cctacctgca ggagatgcgg agcaaacgga atggttttct
     7201 ggaaaaacag gtcgagctca cgatcgggga gagggccatg accctcctga tgacgaccaa
     7261 cgtcattcat gacgatgacg gtcgcgacct gggcatggtt gtcgtctttg aagacctgac
     7321 ccagcttcag aaggcggaac gcgccgccgc ctggcgggag gtggcgcggc gcatggccca
     7381 cgagatcaag aatcccctga ccccggtcca gctctccgct cagaggttgc agaagaaata
     7441 tggagagaag ctgggcgggg accgggaggt gttttttgaa tgcacaaaaa ccatcatcga
     7501 ccaggtcgag gtcctgaaaa acctggtgaa cgaattttcc cgatacgccc ggattcccgt
     7561 tacgaatccg gcgccgcacg acctgaacga ggtcatcgcc gattcggtcg tcctcttcca
     7621 ggatgcccac aaggaaatcc ggttcacgtt cgagacggag gacgcgctcc cgattgtaaa
     7681 catcgacccg gagcaaatca agcgggtcat ggtcaacctc ctggacaacg ccgtggccgc
     7741 cctccgggaa catgacggat gcattgccat ccggacattc tacgacccgg ccgcccagaa
     7801 ggcgagggtg gaggtggcgg acaacggaac gggcgttccg cccagctaca agctgaaaat
     7861 cttcgagccc tatttttcca ccaagggggc cggctccggc ttggggctgg ccatcgtgaa
     7921 ctcgatcatc gccgatcatc atggaaaggt cagcgtccgc gacaacgtgc cgcgggggac
     7981 cgtggtggcc tttgaaattc ccgtgcggga gggagtagcc gtctgagagg aagggtcccc
     8041 gtccggcagg ggcataaacg gtttgggaag gaagcatgcg gaaaacgatc ctgattgtgg
     8101 atgacgaaga aagcatccgc cagtcgctgg gcggtgtgct aaacgacgag ggatatgaag
     8161 tcctggccgc cgccagcggt gaggaagcgc tccggatgat cgacgacgag atccccagcc
     8221 tggttcttct ggacatctgg ctcccgggga tggacggcat cgaggtcctg acgcggatcc
     8281 ggtcggagta tccccagatc cgcgtgatca tcatgtcggg tcacggcacc atcgagacgg
     8341 ccgtcaaggc gacgaaactg ggcgccttcg atttcatcga aaagcccctc tccctggaca
     8401 aggtcatcct cctggtcagc catgccctgg acatggtcaa cctcgaggag gagaacatcc
     8461 tcctccggca gaagctcggc cgggagtatg aactgaccgg ggagagcccg ccgatttccg
     8521 aactgaagga aacgatcggc atcgtggccc acacgaacgc ctggattctg atcatggggg
     8581 agaacgggac gggcaaggaa ctcgtggccc gctccgtcca ccgccagagc cggcgtgcga
     8641 acaagccctt catcgaggtc aactgtgcgg ccattccgga ggatctcatc gagagcgagc
     8701 tgttcggcca tgaaaagggg gcgtttaccg gggcgacggc caagaagcgg gggaagttcg
     8761 atctggccca cgaagggacc atttttctcg acgaggtggc ggacatgagc ctgaaggccc
     8821 aagccaaggt ccttcggatt ctccaggaga agaagttcga gcgggtgggc ggcacccgcc
     8881 tgatcccgac cgatgtgcgc gtcctggcgg cgacgaacaa ggatctggaa caggagatgg
     8941 aggccgggcg cttccggcag gatctctatt accgcctcaa cgtgattccg ttgcgcgtcc
     9001 ccccgctccg cgagcgccgc gaggacatcc ccctgctcgt caaccggttc cttcaggacc
     9061 tggcccagaa ggaaggcgaa gaacagaaga cgatgacgat ggcggccatc ggcgtcctca
     9121 tggaacaccc ctggccgggc aacgtgcgcg agctgaagaa catcgtcgag cggctcgtca
     9181 tcatgacccc cggcaacgtc atcgatgccc gcgacattcc gtccctgaac aaggatgcgg
     9241 aagtcgttct agacagctcc ttttacgatt ccgattcgtt caaggcggcc aaggtggagt
     9301 tcgagaagag ctacatcgtc aagaaactgc gcgagttcga gggcaacatt tcgaagaccg
     9361 ccgaggcaat cggcctggag cgcagcaacc tgcacaagaa gatcaaggcc tacggtctgg
     9421 acgtcaagga cgctggataa gacgtccgtg cccgcgggag ccgatttccg ggcggggttc
     9481 ggggcgcgac gtcagagctg atgccgaacg tccgccaggg aaaagcgctg gaagggaagg
     9541 aaggggaggc ccagggcgat cacgaccccg agcgacagga cgtggtagag gatcgcgaag
     9601 gtcagcgcgt ccgttttggc gacgccgaac agaccgaggg cgagaatgca ggcgaagtgc
     9661 cagttgccga taaagcccgg cgccgcgggc agggcgatgc cgatcacgag gatgatcatg
     9721 acgacgaggg cggcgccgac gggcagcgcc agtccgaagg ccgggaagag gatatagatg
     9781 gcgaagacgt cgaggaccca gatgacgagg gacagagccg cgacggtcag gaaccgcttc
     9841 ccgtcgccga tcatctgaaa gccgtcgacg aaccggtcca cgagacccgc gagttcggcg
     9901 ccgtagggcg ccgggagctt cgccaagagc ggccggagca gccccaaggc gcgctcgcgc
     9961 ttcaggagca ggagggccat cgcggccatc aatccgaccg tcagggcaaa aaagatcgct
    10021 cccgaccgga gcagccagat gggcaggggc gtcaggagcg gcgtcagggc gaagagcgac
    10081 aagaccgtca ggccgtcaaa gacccgctcg acgaagatgg ttcccagcgc ggcgctcatc
    10141 cggatgccgc ttcgctgagc gatcaggtac gggcgggcca gttcgcccag acgggccggt
    10201 agggagacga tggccaggaa tcccacgctg gtgatcggaa acagggtccg ttgacggatc
    10261 tgctccatgg gacccagaat gaccccccag cgaatcgagc ggaaaacctg catgaggagc
    10321 atggcggcga gggccgggag ggcgaacccc caatgcatgg ccgccacccc ctcgcggaca
    10381 ccggcccatg ggatgccccg aacggacaga tagacgaggg cgaggctcac gaggattccg
    10441 gccgcgatgt tttttttcat gcacccgccc tgttcgaggc gtccgccccg tccggcgcga
    10501 cgggggagcg cggatgcggt ccggcgcgcc ccggccgtgt gccgccgggg tacgccggat
    10561 agcctccgtc tgttgagggg atcagttgtt ggcgatgttg tcggcgatct tgtcgtgaat
    10621 cagctgggac gagagcttgt cgccgagaag cccgacgcgg acccccacgg tgatgaggtc
    10681 cttggccttg tatttaacgg tgaactggac cttctcgtcg ttgatgacgg tgctgatcgt
    10741 ccctttgccg atgtccttgt ccggcttgac ctccatgccc cgcatgtcgg caatggtttt
    10801 ttcgcaggcc gcccagaccc tgtcgaacga ggtatagtaa tccgtattca gatccccgtt
    10861 gatgtacagg taggttcccg acccgacccc tacggccgtc accccgacgg ccatggcggc
    10921 gcagccgccc agaagcatca atgacatcat gaggaaggat agcagcgatt ttcttttcat
    10981 cgacaggggc actcctttcc ttgaggttga gggatgacct cccggccacg ggttcattct
    11041 ataatcgatc gcgcccgaat tgtccacccc ttcgtcagac cttatcccgg ggcgcccccg
    11101 aggcgcgcga actcccggag cagctcttcc tgcttgcggg tgaggttcgt cggaatggcg
    11161 acaacggttt cgatgatctg gtcgccgcgg ccgaaaccgc gaaggtgggg gattcccttt
    11221 cccttgagac ggaacgtctt gccgctctgg gttccccggg gaatcttcag cttttcgttt
    11281 ccgttgagcg tcggcacctc caccgttcct cccagcgccg cctgaacgaa ggaaatttgg
    11341 acccggcagt agaggtcgtc gtcgtttctt tcgaagaatt catgggggtc gacgtagatg
    11401 aacacgtaca ggtcgcccgc ggggccgccc gggtcgccgc tttccccctc gccgcgcaga
    11461 cgcaggcggg agccggtttc gacgccggcc gggattttca gctgcaccgt cttggtgatc
    11521 cgggttcggc ccgttccccg gcactgcgga cagggctggg tgatgacgct tccctggccc
    11581 cggcactgcg ggcaggtgct gcggacgctg aaaaagccgc tggactgggt gacctgaccg
    11641 cgcccgtggc agtagcggca gacctccggc gaggtgccgg gcgccgcacc gctcccgtgg
    11701 cagccttcgc accgttcgta cttctcgacg tcgatgtccc gggtcgttcc ggtggcggcc
    11761 tccttgaagg agatcttgag atcgtagcgg aggtcggcgc cggaacgggg acccgtccgt
    11821 ccgcgcgccc ggccgccgcc aaatccgaaa acatcctcga agatgctgcc gaaactgctg
    11881 aagatgtcct cgaaaccgga aaagccatgg tagccggcac cggacagtcc ctcgtgaccg
    11941 tactggtcgt agatggaccg cttctgggga tcgctcagga cctcgtaggc ctcggccgct
    12001 tccttgaacc gatcctccgc ctcgtggtcc ccagggttcc ggtccgggtg gtattgcatg
    12061 gccaggcggc gataactctt tttgatctct tcgtccgagg cgccgcgctc gatctcgagg
    12121 acctcgtaat aacagcgttt catggcttaa cccccggcct gttcgcggag gttgtgttcg
    12181 acgtcttcca gaagcgccgg atcgccgctc cggacggcgg cttcacgcgc gaaccggagc
    12241 ttgcccttcg ccagggccgt gcggcagatc cggacccagc cgtcgggcgt cggctcctcc
    12301 cggagggcct tggcgcaatt caagtatatc atggcatctc cctcgctctc ggcccaggtt
    12361 ctgattttct ccaggccctc ccgatcggcg gcggcctcga aaaactcgag ggcgtcggcc
    12421 atccggccgg cggcgagaaa gccgttcccg tagacggtta ggtcggcgac gcttttttga
    12481 atgtagagca gggcctgttt ttttcggtaa tccggaatgt ctggtcgttt cacggtatcc
    12541 ttaacatgac gcacgccgtt ccacggcgct caaacgggcc taacatagtc accgtcggag
    12601 gcaatgtcaa ccccggggcc gtcaacggtc cgggacgagc ctgcgcaggg cctcttcgta
    12661 tttttccttc gttttttcga tgatgtcccg ggggagcggc gggggcggcg ccgtctggtt
    12721 ccagggaatg gacaggagat aatcgcggag gaactgcttg tcgaagctct tctgcacccg
    12781 cccttcggca tagtcggacc ggggccagaa gcgcgaggag tcgggggtga gaagctcgtc
    12841 gatcaggatc aggtccccgt cgcagatgcc gaactccatt ttcgtgtcgg cgatgatgat
    12901 gccggcccgt tcggcgatct cgcccgcccg ccggtagacg gccagactcg tccggaggac
    12961 ctgttcggcg aggtctcgcc cgatcagggc ctccatgtcc ccgcgtgtga tcgtggcgtc
    13021 gtgttcccct tcgggggctt tggtcgaggg ggtgaacagc ggcttcggga gccgtgcgga
    13081 ctccttgaga cccgcgggca gggtgatacc cgaaacggtc cggtcccggc ggtagtcctt
    13141 ccagccggag ccgctcagat agccccggac gatgcattcc acgggcaggg gccgcgcctt
    13201 gcggaccagc atgctccgcc ctttcaggat gtcccggtag ggacggcagg actcggggta
    13261 cgcatcagga tccgttgaga tcaggtgatt tccgatcagg tccgccatcg ccccgaacca
    13321 gaaggcggac atccgggtga ggatctcgcc ctttccgggg atcggttcat ccataatcac
    13381 atcgaaggcc gaaatccggt ccgtcgcgac gatgagcagg caggggtcga ggtcgtagat
    13441 gtcccgcacc ttgccccggc tgatgagctt caatccggcc aggtccgttt tgttcaatcc
    13501 ggtcggttcg ttcatggggt cctcgacact cctgattctc cggtcacccg tcaccgtcag
    13561 ttgaggaagg ggttgtattt cttctcatgg ccgatcgtgg aggtcggcat gcttccgtaa
    13621 ttgtgacccg gcagaacgag ggtctcatcg ggcagcgtga ggagcttttc ctggatggag
    13681 cgcagcatga gcggccagga gccgcccgga aggtcggacc ggcccacggc ctcgacgaac
    13741 agcgtatccc cggtgaagac gtgtccatcc cggtacaggc acatgccgcc gggggaatgc
    13801 cccggggtgt gcagcaccgc gagtgctacc cgcccgaccg agatgacatc cccctcacgg
    13861 acggtccgat ccgccggggg ggacttcgcc gccccgaaca tcctcagcag catgggcggg
    13921 gtatccacga gcatcggggc atccgcttcg tgaatgatga tttcggcccc ggtcttctgt
    13981 ttcatttcaa ggttgccgga gatgtggtcc acgtgcccgt gggtgttgac gatcgtcttg
    14041 atcgtgagct ttcggctctg cgcgatccgg atcaagccgt ccacattggc ggccggatcg
    14101 atcacgagcg cctcgcccgt gtccgggtcg cccacgatat aggcgaagac cgccatggcg
    14161 ctgacctgca cttgttcgag atacatgctc cacctcccac gcgcccccta tcaggttctc
    14221 gccgggaagt caacgggatt cggccggtgc cgcgctcagc caagtcgccg gaggcgggcg
    14281 ccgaagaagc cgtccatgcc gtcacggtgt gggagcgtcc ggaagtaccc ctcctcgtcg
    14341 agcagcgccg ccggaaaggg gggcgggggc gaatccgggg catactccgg gtggcgttcg
    14401 agaaaatccg cgatcacgtc ctcgttctcc tcgggcagga gggcgcaagt cgcgtagacg
    14461 aggacgccgc cccggcgcgg gtaggcggcc gcggcgtcca ggatgcgccg ctggcgacgg
    14521 acgagggtgc cgaggtcgcg cggctgaagc cgccacttga tttcggggtt ccgtcgcagc
    14581 gttccggtcc cggagcaggg ggcgtcgacg aggacgcggt cgaagcgggc gtggaacgcc
    14641 tccccgaggt ccgcttcggc gttgcgggcc catgttcgga tgggcgtcgc cccgaggtgc
    14701 cgggcgagcg tttcgagatc ggcgagcttc cgggggtgga tgtccacggc caggatttcg
    14761 cccgaatttt gcagcagggc ggccagatgg gtggtctttc cgccgctgcc ggcgcagaga
    14821 tcgaggaggg tttcacccgg gcgtgggtcg acgagggggg cgaccagttg cgaggcctcg
    14881 tcctggatgt ggagcccgcc ttccctgaac ggggcggttt cgcccaaggc gccggcgccg
    14941 gcggcgacgg cgaggccgtc cggggaaaag aacgtcggct cacaggcgag gcccgccgcg
    15001 gccagggcct cccgcgcggc cgcgggggta aggcggatcc ggttgaccct cagggtgcag
    15061 ggcggcgtct cgttgtcggc ccggcagagc gccgccgttt ccccggggcc gtagacggcg
    15121 agccagcggc ggacgagcca gagggggtgg gaggcggttg cggcgatgtg tccgaccgga
    15181 tcctggacgg cgtcgggcca gcggatcgcg tcccgcttcc ggacggcggc ccgcaatacg
    15241 gcgttcacca gggaggcgcg atccgggacg cgtcccttgg cgagttccac cgcggcgtcc
    15301 accgccgcga acgcagggat ccggtcagtg aattccaact gatagagggc cgtacgcagg
    15361 attgcgcggg tgaccggctc caggtcagcg aggcgccctc ggtagagctg acggaggatc
    15421 cagtccaggc gaccctgcac gcggagcgtg ccgtagacga gttgggtcgc cagtccccgg
    15481 tcgcggggat ccccgaggct gtcgcccgac agggcggcgt tcaggagggg ttccgcgtag
    15541 gcccccgttc tttccacgcg cgtcaggaga tcgacggcga cgctgcgggc ggtttcactc
    15601 atgatcccgt ctccgggcgc ggttcgcccc ggccgctcag tcctcctcca cgatcgcgac
    15661 gggccgcgtc cagccggcgg aggcccgctt gatcttgatg gggaagcagg agacggtgaa
    15721 gccgtgcggc cggccgatcg cggagaggtt ggccagcttt tccatgtggc agtacccggt
    15781 ctcgattccg gcgaagtggg cctcccagat cacccggggg tcgccctttt ccttgaattc
    15841 ggcggcgaga aagggcaggg ggcggtccca gctccaggca tcgatgccga cgacgcggac
    15901 gccctgctcc gtcagataga gggtgctttc ccggtccatc ccggccccct tgaccagata
    15961 ttccggcttt ccccaggctt ggtcggcgcc ggtctggacg agaacgatgt cgagcggctt
    16021 gagttcgtac cggatcctgt ccagttccct cttgacgtcc gccacggtga tgcgctcccc
    16081 gtcggccttg tggcggaaat cgagcaggac gccgtcgctc aggcaccacg cgagcgggat
    16141 ctcgtcgatc gtggcggcgg gccggccccg gtccatcttc gggtgatagt ggtagggggc
    16201 gtcgaggtgg gtgccgctgt gggtggagag cgtcaggaat tccacggccc agcccacccc
    16261 gcccggcagc tggtcggcct tgaggccggg gaagaagtcc agcatggagg cggtccccat
    16321 cgcgtgatcg atgtagtcca cccgggggat catcatgggc gggtcgctgg ggagtcccgt
    16381 ttcgagcgcg atgctaaggt cgacgaaacg ttttttcttc atggacggtc tcctttctcg
    16441 tgaagggttt gcgattgtcc ggcggacgcg tctatcccag gcggctgccg gacgcgagcc
    16501 tctgcccccg caggaaatcc ccgatcggca ggcgcttctt gttttccagc tggacctccg
    16561 tcaggcagac ccggcctccc cggacggcca cgggcaggcc cccgggcatc gcttccccga
    16621 ccgttcccgg cgcgtcggcg acggggcccg gcgacgcctc ggcccggaac accttcagaa
    16681 tcttcccgtc gcagcgggtc caggcgccgg gtgtcgggga aagcccccgg atcaggttga
    16741 ccacggcgtc cgccggtgcg tcccagcgga tcaggccgtc ttcccggccg attttgggtg
    16801 caaatgtcgc cccggaaggg ttctgggggg ttcccgtgag ggttccgctc acgattccgt
    16861 ccagggcgcg cgccaggagt ccggccccga ggatcgccag acggtcgtgg agcgcgccgt
    16921 aatcctcccc ggggtcgatc ggggtttctt cctggatcag gatgtcgccc gcgtccatcg
    16981 cctcgttcat ccgcatgatc gtgacgccgg tgacggactc cccccggatc agggtccagt
    17041 tcatcggggc ggcgccccga tacttcggca ggagggaggg gtggacgttg atgcagccga
    17101 aacgggggat ctcgaggagg accgccggga ggatctggcc gaacgcggcc acgacgatca
    17161 cgtcgggcgc cagggcgcgg caggtgtcga gaaacgccgg gtcccgcacc cgctcgggct
    17221 ggaggacggg cagtcccagg cggagggcgg cgtccttcac cgggggagcg atccgggacc
    17281 gtcccctgcc ccggggccgg tcaggctggg taacgacggc ggcgatcggc cagccgttta
    17341 cggcgaggcg ctccagggac ggcagggcga actccggcgt tcccatgaac atcactgacg
    17401 tctgcatgcg catcctgcgg ttcccggccc gcgggttata gaaagtgcac cgggtcgacg
    17461 tcgatccgga cgtccagacc cgtgtgcgcg gcgtgttcgg acaggctccg ggccagagcg
    17521 tgcaggcttg taacatgttt gcccttcaaa agcaattgcc agcggtaccg gcccttgatc
    17581 ttcgccacgg gggcctccgc tggaccgatg acctcgatcc ccggcgccga ccgtcctgcc
    17641 gtggccagtt cccgggcgag acggcccagt gtttcaccgg ccgcccggcc cttttcccgg
    17701 gagcggcagg agaggtgaag actcacgagg cggctgaacg gtgggtagga cagggcttcc
    17761 cgggaggcga tctcctcctg gtagaatccc gtgtagtcgt gatgccgggc tttgagcagg
    17821 gcgtagtgcc ccgggttgaa ggtctggatg aggacccggc ccggcgtctc cccccggccg
    17881 ccccggcctg ccacctgggt gaggatctga aacgtccgct ccgccgcccg gaaatcgggg
    17941 aggttcatgg aggtgtcggc cgagataacg ccgaccaggg tcacgcccgg gaagtcgtgc
    18001 cccttggcca ccatctgcgt gccgatcagg atgtcgatct cctgcccggc gagccgctgg
    18061 aggatctcga tgtgggcgcc ccggcgcgtc gtggtgtcgc tgtccagacg ggcgatgcgc
    18121 gcctcgggga acgtcgcccg gacctccgcc tccacttttt ccgtccccac gccgaaaccg
    18181 ccgaggcggg aacttccgca ggccgggcag gtcggcggaa cggggtggcg gaagccgcag
    18241 taatgacatt tcaggatggc gtccccgccg tagtaggtga gcgagacggc gcaattgagg
    18301 caccggaagg cctggccgca gtccaggcag accatgaacg catgcgagcc ccgccggttg
    18361 agaaacagga tggcctggcg gccgaggctg agcgtctccc ggagcgccgt ctgcaggggt
    18421 gccgagagga cggccggctg gccggccgct tcccgccgtc cccgcatgtc gatcacctcc
    18481 acttggggca ggggcttgga caggacccgg tcgggcaggg tcaggaggcg gtatttccgc
    18541 gcggcgatgt tgaagtagga ttggagttcg ggggtcgccg aacccaggat caccgccgct
    18601 ccggccagtt tggcccgtat gacggcgagg tcgcgggcct ggtaatgcat ccggtcgtcc
    18661 tgtttgtagg acaggtcgtg ttcctcgtcc acgacgacca gcttcagatc ccggaccggg
    18721 gcgaagaggg cggaacgggc gccgatgatc agacggaggc ggccctgacg gatctgccgc
    18781 cactggtcgt aacgggcggc gcgggagacg ccgctgtgga ggaccgcgac ggcttggcgg
    18841 gggaaccgtg cctggagacg gttcaggagt tggccagtca aggcgatttc cggcaccaag
    18901 agcagggcgc cgccgccgcg ctccaggacg tccgcgatgg cccgcaggta aacctccgtc
    18961 ttgccgctcc ccgtgacccc gtggagcaaa aacggcgcaa accctccccg gttcaggctc
    19021 tcccggatcg tttcaacggc gttccgctga tcggggttga ggacgggaac cgcctcggtt
    19081 gtccggacgt tttccccggg cgcttcgccg ccccggcggg gggcaagccg gcgttcggcc
    19141 tccgtgacgc gcacgaggcc ggcgtcctgc aactcccgga tttcctttcg gatgtcttca
    19201 atgccggtga ctttccgcag gcggttcagg gtgagaccgt tccggcgccc ggccaggacg
    19261 gcgcagagga cgtccgccgc cggggagaga agggcggtga tccggtccgc ggcgtcttcc
    19321 gtgaggcgga tgcgctggcc gccggatcgg tattccgttc cgggcaggac gtcggccagg
    19381 gccttcccca gggggtagag ataataggag gccgcccagc ggtaaaagcg gagttcttcc
    19441 ggaccgaaca ggggctccgg gtcgaggatg tcgaggatct cccggatttc ctttccgccg
    19501 gcatccgggg cgacgtcatc gacggcgagg atgacgccga cctgccggcg acggccgaaa
    19561 ggaacccgga cgcgctttcc cggggcaacc tcggccgcaa ggaaggaggg aaccctgtag
    19621 gtaaaggttc gctcggaggg gatgtcgatg gccacctgga tgaacatgag cgtatccggt
    19681 tcgcgtgctg ggcgccgggt gggactcgaa gaaaggccac cgggatgaga gggaaggacg
    19741 tccggccggg gcattcgcgg ttgcgggccg catcggcccc cgttttcccc ggcgtccgga
    19801 gacaagaagg ggctactccc ccttgagttt ttcgagtttt tcctgcttcg tcttgcagtc
    19861 gatgcacagg gtggtgaccg gtctcgccac cagccgcttt tccgagatgg gtccgccgca
    19921 gacctcgcag ttgccgaagc tgccgtcgtc gatccgcttg attgcttcct tcatcttggc
    19981 gatcagtttt cgctcccggt cccggatcct aagttcgaaa ttccggtcgg attccaggga
    20041 ggcgcggtcg ttgggatcgg gataattctc cttcccgctg gtcatctcgg aaaccgtttt
    20101 ctcagcgtct tccagcagtt cgttgatctt ctgcttgagc atgtatctga aaaactccag
    20161 cttttccggc ttcatgggag ccatcacata cccctccgtg gcggacaacg catcgaatgt
    20221 cttttccggt ggcagcctca tacatgatgt ttcccctttt gtaaagtaaa aagatcgcgg
    20281 tgcggtgcgg cttagttttt gccccgggcg agcggatgtg gtatgcaaaa tcccggaggt
    20341 ggaaaatgca gagaccggag atccggaacg tcgtctcgcc gcgccggcgg accgccgcga
    20401 acggcgcagc cgggtcgcgg gaagacgtct ccgtcgtgct gacggccatt gccgagggat
    20461 ttctgggcgt gccctacctc gcccatccgc tggatcaaac cggcccggag catctggtcg
    20521 tcaacctgga cgcctttgac tgcgtcacct ttgtcgagac ggcggtggcc ttggcccggc
    20581 tcccggcgga cggacgggcg gatcgggagg gactggcgcg cgccgtcgag gaaatccgtt
    20641 accggggggg gcgcctggac ggctacgcct cccgtctgca ttatttttcc gactggctgc
    20701 gggacaacaa ggccaggggc atcctcgagg acatgaccgc gcggctggga gggacgccct
    20761 gggaaaagcg gctgaatttc atgacggccc atcgcgaccg ttacccgccg ctcgccgatc
    20821 cggaatgcta tcggcgggtg cgcgccgtgg aggaggccct gacggcaatg cccaaggtcc
    20881 atctgccccg cgggcgcttc cgttcacagg aggcgcggat tcacgacggc gacattctgg
    20941 ccgtgacggc gttgcaggac gggatcgacg tcctccatgt cgggttcgcc gtgcggcgcc
    21001 gcggtcgaat ccatctgctg cacgcctccg aggcggccgg ccgggtcgtc gtctccccgg
    21061 aaacggtcta tcgctatctg caccggcgcc gcgcgcggac cggtcttctc gtcgcccggc
    21121 tccgtgcggg ggccggcctt cctacctcag gacgaccagc agggcccgcc gggacgccga
    21181 cgtgaggcgt gtcagcgtgg cgtcgcaggt cgtttcggat tccagcggtt gaagcatctc
    21241 gacacgaagg gtccgcgtca cgccggacca ggcgtgacga agctcgtcct tgaaggcatc
    21301 gcggccggga gcggccagaa gatccgtcag gctggcgccg atcatctccc cgcggggttt
    21361 gcctgcgtat tccagggcgc gttccgtgat ctcttcgacc gttccctccc gatccacgac
    21421 aaaaccgatc tcttcccggt cgcggtgatt gagcaggcgg tcccggttgg cgcgcatcca
    21481 gtcataggcc gcggtcatcc ggtcgttggc gtccttgagc tcgacgttca cccggcgaag
    21541 atcctcctcg acccgctggc ggtccctgac ttcgcgctcc agcgtgacgt tcttcgccag
    21601 gatctcctcc agctgggcct tgatctgcga gtgaagcctg gcgttctcga gcgcaaagct
    21661 cacgcttccc agcatggtgg agacgatccg ttcgtccaac tcgtcgaaga gcagcccgga
    21721 ttccttgttg gcgaggttga gaacggcgat cagggcatgg tcggcgaaaa tgggcatgct
    21781 gaggaaggag ggggggccgt acttcggatc gttgggcttg agcgttctcg gatcgctctc
    21841 gacgttctgg acgatgatgg ttttccggtc ctcgatgacg cgcttcatga aggaccgctc
    21901 gagattgacg gaggt
//
LOCUS       DAOOLJ010000028        21327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2420_length_21327_cov_16.421493, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000028 DAOOLJ010000000
VERSION     DAOOLJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 21327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21327
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_21327_cov_16.421493"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLW16_06240"
     CDS             complement(<1..227)
                     /locus_tag="PLW16_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70538.1"
                     /translation="MTGKERILTALALKEPDRVPLYIHAINEAPIIGIGRHLREGLPE
                     VKTLYEMDASETVTLLDTLFLIHEAFEIDGFT"
     gene            complement(247..879)
                     /locus_tag="PLW16_06245"
     CDS             complement(247..879)
                     /locus_tag="PLW16_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPB70539.1"
                     /translation="MPLLSDLAHALEAGDRSRVEDLTARALQENVSPEGILREGLFAG
                     MDVVGARFKSGDIYIPQVLIAARAMHAGLEILRPHLIRTGAKPAGIFIIGTVQGDHHD
                     IGKNLVAMMLQGKGFEVIDLGVNVPPERFVQALRDHEANLLGLSALLSITLPFMKETI
                     EAICRAGLRDSVKIMVGGGPVTRAFADQIGADGYAPDATTAAENALALLS"
     gene            complement(1144..2124)
                     /locus_tag="PLW16_06250"
     CDS             complement(1144..2124)
                     /locus_tag="PLW16_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70540.1"
                     /translation="MSILNTVTGPVASDRLGKTLIHEHFVFGYPGWEGDVTLGPFDRE
                     ACVRAGLEMAAKVQAHGVSTVVDATPNETGRNPLLLKEISEKTGLNIICASGYYSEAE
                     GATPYFKLRSKFGDGAQQIYEMFKKEVAEGIGTTGIRPGVLKLASSKGEITEYERMFF
                     RAAARVSREEGIPILTHTEEGTQGPEQAELLLAEGADPGRVLIGHICGNTDVDYLMRV
                     LETGVYIGFDRLGIEGLVGAPPDSRREACLIGLIGLGYAQQIMMSQDWVNHWLGRPGV
                     AEIVSMVMPNWKPTHLFDDVLPVLSKGGVTAGQIETMLVDNPRRFLAGGS"
     gene            complement(2147..>3772)
                     /locus_tag="PLW16_06255"
     CDS             complement(2147..>3772)
                     /locus_tag="PLW16_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB70541.1"
                     /translation="TGVIDVNYNGELQTAWACRFGTLTEILKAGVARFGDKEMWHFPD
                     FDIRWSFNDFNAAVNNVAYGLQEDCGIRKGDRVALMANNVPEAYVCYLALSQIGAISV
                     IINARLAPEEAARQLQDAGSIAVVIEAALWDGMQPVVESLPGLKAVFVSGRTPLGRAK
                     AFSELMDRPAPREIRVDVSERDICSLIYTSGTTGRPKGVITTHRALVNNAMNGLSCAE
                     GIYHARPEDWRQLIVTPFFHVTTLHTLINFTLLGATAVVMSTFKPKEALDLVIDEEIN
                     FMVIVTAMYWILKLQPRYPELVKANRLKYILQGGSPMPPEMFRELFKEFPDARVGNGY
                     GFTEGASLGWLMFMAGDWETLIQKAGSVGGTLGNSLLRIVDEKFKDVPRGQTGEILAA
                     SPGISPGYWNLPGETAQSYVVDADGRRWFKTGDLGYTDADGYTFLVDRAKDMICRGGE
                     NVYCVELENLLSQNAKVLEVGVVGVPDPVLGEKIKAVIFPIPGETPTEEEIRAFCRGR
                     IAEYKLPDYIVFTNQMLPKNPGGKLMKKELKNL"
     assembly_gap    3773..3872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3873..4654)
                     /locus_tag="PLW16_06260"
     CDS             complement(<3873..4654)
                     /locus_tag="PLW16_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPB70542.1"
                     /translation="MDYLDKTKPIRAGEEFDVKKVEAFLKDAIPGLQGELTVAQFPGG
                     YSNLTYLVKVGEREFVFRRPPFGRKAKSAHDMGREYRILKALKPVFPYCPAPILYTED
                     ESVIGCPFFVMERLKGVLLRRDLPPGLTYTPEQTWQLCENVLDVHVALHKVDYVRAGL
                     ENFGKPAGYVKRQVEGWSERYRQARTPDAPVYEDVMDWLTKTMPGDSGIVSVIHNDYR
                     FDNVVLAPDNPPLRVLGILDWEMATIGDPLMDLGGTLAYWIDR"
     gene            complement(4770..5474)
                     /locus_tag="PLW16_06265"
     CDS             complement(4770..5474)
                     /locus_tag="PLW16_06265"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB70543.1"
                     /translation="MMTFAEFRELVNLSMEALCRDFFRENPDRVRVKKEATVVRNLVK
                     ILDATLMISNQKGFQAMSVRDLSRETGLSMGALYSYISGKEELLAIIRAQGYRIVVRI
                     LTGAIAEVSDPHEKLCQAIQTHLYLSEAMRAWFYFSYMETKNLSKEEQKKSIQMELFT
                     EKLFIDILSAGCRAGRFRPLNVELTAAAIKALLQDWYLKPWKHQRRKVTIEEYARFVT
                     GLVEAYVLPFPAAASP"
     gene            complement(5526..6734)
                     /locus_tag="PLW16_06270"
     CDS             complement(5526..6734)
                     /locus_tag="PLW16_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70544.1"
                     /translation="MDFTVSEKMQAVVGMMNEFVGRELIPLEPEFLTKSFRQMEPVME
                     GKRQMVRQMELWAPNHPPEYGGMGLNLMDHALVSEALGRTPIGHYTFNCHAPDAGNIE
                     ILHKYGTGAQKERWLRPLIEGKIRSCFSMTEVELAGSNPVMMDTTAVKDGQDYVINGQ
                     KWYTTGADGAAFAIVMAVTNPEAAPHMRASMIIVPTGTPGFNLVRNIPVMGHSGDSLF
                     SHGEILYQSCRVPQENLLGPEGWGFVIAQERLGPGRIHHCMRWLGICHRAFDLMCARA
                     SKRVISYDNKTLASKQIIQSWIAECAAEIQAARLMTLHAAWKIETLGVKEAREDIALI
                     KFYVANVLQKVVDRALQVHGGLGMTDDTIIAFFFRHERAARIYDGADEVHKATVARRI
                     LKGYAGRDVR"
     gene            complement(6802..7512)
                     /locus_tag="PLW16_06275"
     CDS             complement(6802..7512)
                     /locus_tag="PLW16_06275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB70545.1"
                     /translation="MSAIYMIRHGQASFGNHNYDQLSERGVLQSRLLAQYLKRIGFQC
                     DAACAGRMVRQQDTARETLDLYARDGLAFPALTVDEAFNEYNSASIILSHVQELARED
                     RSLDADMKQIYTDRKAFQRVFERIMLKWIAGEDRKEGLDSWETFRDRVWGGLKQIMAA
                     HGKGRTVIIFTSGGPISAAMQLALGISDEQTLRIAWQIVNASITKFVYNEERISLAAF
                     NQTAHLDLERDASLMTYR"
     gene            7860..>8398
                     /locus_tag="PLW16_06280"
     CDS             7860..>8398
                     /locus_tag="PLW16_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB70546.1"
                     /translation="MENIAEHFLHRFFYPASIALIGASENVFRPSYHLVSNLLKLGYA
                     GPIYPVHPKQKEILGIKAYPDVGRIEAVPDLAVIAVSQAQTPGLLDDCIRKGIRRVVL
                     VAGGFSEIGADGRRLQEEMAGRVRASGVRAIGPNALSPINPHIGLAVSFHALDRLHSG
                     GLSLIFQSGLYEPRLRWLF"
     assembly_gap    8399..8498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8499..9143
                     /locus_tag="PLW16_06285"
     CDS             <8499..9143
                     /locus_tag="PLW16_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70547.1"
                     /translation="RVFDRVLKQVGAIRAESIDEFFDLCRALERFDGLALAGNRVVIA
                     TMPGGEAVVMTDRVQQEGLTMARVSAGTLERLRPVFPPWDMPANPFDLGVTMQFGNPV
                     TVFETLVASLAADPGVDAAHLQIPDLLLGLPRETFGMFFPLPEAGKPLVLWVAGMEPG
                     DHETLAWLEDHRIPVFPSPEKAIRVLRALHRLQERSRRLRPETGPPSRQDDRPS"
     gene            9140..>10581
                     /locus_tag="PLW16_06290"
     CDS             9140..>10581
                     /locus_tag="PLW16_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70548.1"
                     /translation="MNGEAHRYDPQTVTGIFLQQARRYGDRTLKLAKRNGRWTPTTWR
                     EALAGLRAGVMGLVALGTAKGDPVGIMSRTRAEWADADLAILSAGGVTIGLYPTASLK
                     ETTHIIRHSGLRCCFAEDDAILETLLSIRETLGQPETLVVFATRRTSLPPGVLTWDDF
                     KAEGRILDGRAPERFEAICRAVRPEDLATIAYTSGTTGPPKGAMITHANLYHTVLNAT
                     QMHHYEESDFGLAFLPLTHMLQRMTVYAAMHLGIVGAYAESIDRLVDNFRELKPTVQV
                     SVPQIFERIYNRIQQMLAGGSPLKGRIFRWAVDVGHRAARVRRDGRRLPPGLALAHTL
                     AHRLVFRKIHAVFGGRVKYLLCGGAPMPLYLLEFFEAVGLLILEGYGLTETVAPVAVN
                     RAERYKLGTVGQLIPGMAAKIGDDGELLLKGKGLFQGYYRDPEATAAAIDGEGWFHTG
                     DLATLDAEGFITITDRKKDLIVTAGGKNVAP"
     assembly_gap    10582..10681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10910..>12288)
                     /locus_tag="PLW16_06295"
     CDS             complement(10910..>12288)
                     /locus_tag="PLW16_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB70549.1"
                     /translation="LVNPKGGQIAGRPVYPSLGDIPGPVDLVVVTVPAEAVIALLPEL
                     KAKCVKSMLLVTSGFGETGPEGKELERKLVAAAHQAGVLILGPNTMGICNPHAKFFCT
                     GMHARPVPGTLAFIAQSGNLGTQLLAFARKEGIGIRAFSGSGNEAMITIEDYLDAFAV
                     DALTRTVLLYIESVKNGRRFFQAARAVSRQKPVIVLKGGRTREGNKAAESHTGALAAN
                     NRVFEAACRQAGVVLVEHPMDLIDVSAAFSSLPLPRGNRVGIATLGGGWGVVAADLCV
                     ENGLELAPLSADLIARIDRILPPYWSRSNPVDLVGEADLTVPLRVMEEMMRWDGCDAY
                     IHMGVMGRRIMVQWLAESATAVDPSQNKALIEERLQKVVDYEKFFLESMVRLMEKYGK
                     PILGVYLLTDEKSRTITDVEGSPFKGVVYLTPEQAVKALAKMHAYGQWLEREGIPAGE
                     RGVRVPGA"
     assembly_gap    12289..12388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12389..12860)
                     /locus_tag="PLW16_06300"
     CDS             complement(<12389..12860)
                     /locus_tag="PLW16_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB70550.1"
                     /translation="MTKIDTLIQQVITDGRTTLTEAESKTVLKAYRAPVIEEWVVKTP
                     AEAVRQAEQTGYPIVLKGLGAKLTHKTERGLVKLNLGSAAAVNQAAFEIEKGAGPDLE
                     GYLVQPQVTGRREFVAGLFRDAQFGPVVMFGLGGIFTEALDDVVFRLAPFDEGMA"
     gene            13120..>13604
                     /locus_tag="PLW16_06305"
     CDS             13120..>13604
                     /locus_tag="PLW16_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPB70551.1"
                     /translation="MSVRYAILGLLHYRPMHGYEIKRHIERHFGHMWTINYGQIYPNL
                     KSLESEGLITLTHVSPSEKGGPNKKLYAVTPEGRDNFAQWLESAEDSRMFLRDPFLMR
                     FIFFGFGSKSQAIATLDRQIAQYEDQYRRREQHVARWQERGAYVRLIAELGVEFNGLY
                     V"
     assembly_gap    13605..13704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13705..14542
                     /locus_tag="PLW16_06310"
     CDS             <13705..14542
                     /locus_tag="PLW16_06310"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB70552.1"
                     /translation="LGVEYVPADLTRPETLPPLFAGGVDRVFHLGAICNFSTPYARLH
                     PTNVAGVERFTKLAQDAGVKRYVHVGSTSVYGPYRGVPFREDGPRDPQDDYGRSKRDG
                     EDVVWARIREGFPAVITRPCTVYGPRCNDGAGKAFSRPTAIAAIPGHGRQRLSNIRAE
                     DVAAAVEHLSHLDEAIGQAYNIAEDTNPTVEEALTLAAETFGTKPPTLRLPLWLLKIV
                     ARIDGGLAVRQGRIPDLEYDAVRYLYDDYIVDNTKLKATGYRLRFPDFRASMAQLKDV
                     GR"
     gene            14620..15459
                     /locus_tag="PLW16_06315"
     CDS             14620..15459
                     /locus_tag="PLW16_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB70553.1"
                     /translation="MGQVVVITGLADGMGREAAKLLAAAGDTIAGFDMDAAGIASLQT
                     ELDGLSPSSGKTHYLVPLNILDRPGILRFRDEVLAKYKRVDTVLSNVGIGFFGPFEEI
                     DLEAARRCLEINVIGAAAIFQAFLPAMRRQGSGKLIAMSSLVGQVPFPFESIYSASKF
                     AIEGMVMSLRYEVAPFGIRVALIEPAQVSTTFAAKIHTLPPEGSPYRERARRFIARDD
                     ELIKTAPTPLQAARRIAEIIHQDRPDFHNQVDFMSTFFLFLNRFLPVRLRDMILLNHM
                     DIQ"
     gene            15506..17311
                     /locus_tag="PLW16_06320"
     CDS             15506..17311
                     /locus_tag="PLW16_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPB70554.1"
                     /translation="MAEKFISERNLKFLLNEVFDTPSLVRHPYYADHSPETLDMVLDT
                     AVRMARDTLKPCFTEMDRNPPVFADGKVHVHPMVRQILKQFGEGGWISAMASTEHGGQ
                     QLPMIIVMATHFIFGAANYSGSIYPSACTGAAHLITSFGTPEQIATYVPRLYAGEWQG
                     TMALTEPQAGSSLADITTTAEPTAEGDYRIKGQKIFISASEHDGADNIVNLMLAKIKG
                     APAGIKGISLFIVPNKRLDGAGNLVPNDVTVTATYHKLGYRGTPINQLSLGDNDDCRG
                     ELVGLPHKGLSYMFQMMNEARLHVGIGATAIASAAYYAALEYAKARPQGRRPTDRDPL
                     SPQIPIIEHADVKRMLLFQRSVVEGSLALLLQCSKYADLNLSAPEEEKERAALLLDLL
                     TPVAKTYPAEMGILSTSQGLQCLGGYGYCEEFPLEQHFRDARIHPIHEGTTAIHGLLL
                     LARNVTMKNGRAFTLFLEEVGRVIAATDTDEGLKPYGRQLAEALERLKNVTNHLLGLA
                     MQGSIDLFLADATLYIECFGIIAVAWQWLVQAQVARTALAKGPAAEEANFYQGKMYAF
                     RYFFGYELPKIAGLATRLLNSDGLTVEMKEDFFND"
     gene            17322..19703
                     /locus_tag="PLW16_06325"
     CDS             17322..19703
                     /locus_tag="PLW16_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HPB70555.1"
                     /translation="MSQPTPPVYEVPKTRIGRLRERIIHAPQEVCIERARYLTEAMSR
                     HWDRPPLTRMSLALEHILANISVVIRDDELIVGSRTSKLKGAPLFPENKSRWIAGDVD
                     NFDRRVLQRALITEAEKRELETAILPFWEGRTVEDLFEARLPEDIAADMDKYIFTMML
                     EITYGIGHFTMNHRRILREGLGGTIATAAARREALAPAEQAGEKGLFYDAVIRSLRAA
                     VTFANRYAALARTLAAEAASPKRRAELLEIAGICERVPEHPARTFAEAVQCVYFIHLI
                     SQIESGGNSISLGRIDQILYPYYAADLAAGRITPEAARERLALLFIKTNEIWNVLEEA
                     FIPGGEGTEGKTTQNVTVGGMDARGEDATNALSEIGLDAFADIRTVQPNFGIRLCGKT
                     PLAFFQKAVRYAKDAVPLHLFNDDAIVPALVRSGHTLEDARDYGVVGCLEPNAQGKSF
                     CSTFAVQFNGIKCLEFALSNGTDNIFGYASGIETGDPATFTTFDEVWRAYDRQMTHFI
                     GQMTRGMAVLDEAIAERVPSPFASAMIDGPLEKGLDLTRGGAVYNATGVQLMGFANVA
                     DSLRALEKAVFTDRVCTLSELVGWLSDDWMDTEDKRLYFLDKIPKYGNDDDAADAMAA
                     RVMAHFCDTVSARRNFRGGSFWPGIFSVGFHLAFGSFTGATPDGRSAGDILGNGVTPA
                     NGRALNGPTAVFNSVTKLPLAEATNGVNLNMRFSGRRLKPEILLALIRGYFRRGGMQV
                     QFNMIDSDVLRDAQAHPDQYRDLVVRISGYSVTFVDLSDTAQDEIINRMAFAL"
     gene            19876..21105
                     /locus_tag="PLW16_06330"
     CDS             19876..21105
                     /locus_tag="PLW16_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70556.1"
                     /translation="MTSERKIFYGWWIVGGAFFLNFAGIGIIMNTMGVFIKPVTESLG
                     FTRGGFTLYFTIAALSMMVMAPVMGKLLERYNIRLIMTVCTAMMATSFALFSQCRTLT
                     QFYIVAVFLGIGSAGSHIIPVSMMITNWFIDKRGLAMGIVFAATGVGGMIFNPLANWI
                     ILNHSWQAAFLTFGLVIGLASIPTALFIVRAKPADMGLLPYGGEAALARQSAAEQGGL
                     TATKAFRTGAFWLLALIILLIAVANMGVLHHIVPYLTDLGFSSTTATTLMSLHMAMLI
                     FGKVLAGSLADRLGLLKSYLILMVGLMVSIALLYGSQWMWVAVVFNILFGFSIAVRTV
                     LPPLMTARVLGQKHFAVIYGFLNIFTTLGTAVGVPLSGFIYDATKSYYLAFALYIGLC
                     LITTAAGIAVLTRSGKK"
BASE COUNT         3646 a         7018 c         6408 g         3755 t
ORIGIN      
        1 gtgaagccgt cgatctcgaa cgcctcgtgg atcaggaaga gggtgtcgag cagggtgacc
       61 gtttcggagg cgtccatctc gtagagggtc ttgacttccg gaagtccctc cctcagatgc
      121 cggccgatcc cgatgatggg cgcttcattg atggcatgga tatagagggg aacgcgatcg
      181 ggttccttca gggccagggc cgtcaggatc cgctcttttc cggtcatagc gatctccggt
      241 ccgggtctat gacagcagcg ccagggcgtt ttcggccgcc gtcgtggcgt cgggcgcata
      301 gccgtccgcg ccgatctgat cggcaaacgc ccgggtcacg ggcccgccgc cgaccatgat
      361 cttgaccgag tccctcaggc ccgcccggca aatggcctcg atcgtctcct tcatgaaagg
      421 gagcgtgatg gagagcaggg cggacaggcc aagcaggttc gcctcgtgat ccctcagggc
      481 ctggacgaac cgctccggcg gaacgttgac gcccaggtcg atcacctcaa atcccttgcc
      541 ctgaagcatc atcgcgacga gattcttccc gatgtcgtga tgatcgccct ggacggtgcc
      601 gatgatgaag atcccggccg gttttgcgcc ggttcggatc agatggggcc tcaggatctc
      661 caggccggcg tgcatggccc gcgcggcgat cagaacctgg gggatataga tgtccccgct
      721 cttgaaccgt gcccccacga cgtccatccc ggcaaacagc ccctccctga ggatcccttc
      781 gggcgacacg ttttcctgaa gggcccgggc ggtaagatct tcaacccggg agcggtctcc
      841 cgcctcgagg gcgtgggcga gatcggacaa tagcggcatg cggggcacct ccgggggggc
      901 gagggtcgaa acaggtcctc tgccccaggg acgcttcacc agtaaagaag cccgcgcctg
      961 aagtcaacaa gaatctccgc cggacaggcc ggcgccggcg tttccggagc cgtcacgcgg
     1021 ctgcccggca cgaccgcaac cccgccggac cttcgggagc cccagccgcg cctcatgaaa
     1081 aaggccggac acgatccgtt cccgccccgt gtccggccgg catatttcag accagcagca
     1141 caatcacgac cctccggcga gaaaccggcg ggggttgtcc accagcatcg tctcgatctg
     1201 gccggcggtg accccgccct tcgaaagaac cggcaggacg tcgtcgaaca gatgggtcgg
     1261 cttccagttg ggcatgacca tcgagacgat ctcggccacg cccggccggc ccagccagtg
     1321 gttgacccaa tcctgcgaca tcatgatctg ttgggcgtac cccaggccga tgagaccgat
     1381 caggcaggcc tcgcgacggg aatcgggcgg cgctccgacc agcccctcga tccccagccg
     1441 gtcgaagccg atgtacaccc ccgtttcgag gacgcgcatc aggtaatcca catccgtgtt
     1501 gccgcagatg tgcccgatca ggacgcgccc ggggtcggcc ccctccgcca ggagcagctc
     1561 cgcctgctcc ggcccctgcg tgccctcctc ggtgtgcgtg aggatgggga tcccctcctc
     1621 ccgggagacg cgggccgccg cccggaagaa catccgttcg tactccgtga tctccccctt
     1681 gctcgacgcc agtttcagga cgccggggcg gatgccggtc gtcccgatgc cctccgcgac
     1741 ctccttcttg aacatctcgt agatctgctg ggcgccgtcg ccgaacttgc tccgcagctt
     1801 gaaatagggc gtcgccccct ccgcttcgga gtagtagccc gacgcacaga tgatgttcag
     1861 gccggttttc tcggatattt ccttcaataa caacgggttg cgtccggtct cgttcggcgt
     1921 tgcgtccacg acggtgctga cgccgtgggc ctggaccttc gcggccatct ccaggccggc
     1981 ccggacgcag gcctcgcggt cgaaggggcc gagggtcacg tccccctccc accccgggta
     2041 gccgaaaacg aaatgctcgt ggatcaaggt cttgcccaga cggtcggatg cgaccgggcc
     2101 cgtaacggtg ttcaggatgc tcatggtttc cctcctttgg acggacctaa aggtttttca
     2161 attctttttt catgagtttc ccaccaggat tcttggggag catctggttc gtgaacacga
     2221 tgtaatccgg aagtttgtat tcggcgatcc gcccccggca gaacgcccgg atctcctctt
     2281 cggtcggcgt ctcgccgggg atgggaaaaa tgaccgcctt gatcttctcc ccgagcaccg
     2341 gatcggggac gcccaccacg ccgacctcca ggaccttcgc gttctggctg agcaggttct
     2401 ccagttcgac gcagtagacg ttctctcccc cccggcagat catgtccttg gcgcggtcga
     2461 ccaggaaggt gtacccgtcc gcgtccgtgt agccgaggtc tccggtcttg aaccagcggc
     2521 ggccgtccgc atcgacgacg tagctctggg ccgtttcccc gggcaggttc cagtaccccg
     2581 ggctgatccc ggggctcgcg gccaagatct cgcccgtctg cccgcgcggc acgtccttga
     2641 acttttcgtc cacgatccgg agcaggctgt tgcccagcgt gccgccgacg ctgcccgcct
     2701 tttggatgag ggtctcccag tccccggcca tgaacatcag ccacccgagg gacgcgccct
     2761 cggtaaaccc gtacccgttc ccgacccggg cgtccggaaa ctccttgaac agctcccgga
     2821 acatctccgg cggcatcggc gagccgccct gaaggatgta cttcaaccgg ttggccttca
     2881 ccagttcggg gtagcgcggc tgcagcttga ggatccagta catcgccgtc acgatcacca
     2941 tgaagttgat ctcctcatcg atgaccaggt ccagggcctc cttgggcttg aaggtggaca
     3001 tgaccacggc ggtggcaccg agcagggtaa agttgatcag ggtgtggagg gtggtgacgt
     3061 ggaagaacgg ggtcacgatg agctgccgcc agtcttccgg gcgggcatga tagatgcctt
     3121 ccgcgcaact gaggccgttc atggcgttgt tgacgagcgc ccggtgggtc gtgatgacgc
     3181 ctttgggtcg tccggtcgtc ccggaggtgt agatcaggct gcagatgtcc cgttcgctga
     3241 cgtcgacccg gatctcgcgc ggggccggcc gatccatgag ttcggaaaac gccttcgccc
     3301 gtcccagggg cgtccgcccc gagacgaaga ccgccttcag cccgggcagg gattcaacca
     3361 ccggctgcat gccgtcccag agggcggcct cgatcacgac ggcgatactc cccgcatcct
     3421 ggagctggcg agcggcctcc tccggcgcca gccgggcgtt gatgatgacg gagatcgccc
     3481 cgatctgcga cagcgccagg taacacacgt aggcctccgg cacgttgttc gccatgaggg
     3541 cgacccggtc ccccttccgg atgccgcagt cctcttggag accgtacgca acgttgttga
     3601 ccgcggcgtt gaaatcgttg aaggaccagc ggatgtcgaa atcggggaag tgccacatct
     3661 ccttgtcgcc gaagcgggcg accccggcct tcaggatctc ggtcagcgtg ccaaagcggc
     3721 acgcccaggc cgtctgaagt tccccgttgt agttcacgtc gatcacgccc gtnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgatcgat ccagtaggcc agggtgccgc
     3901 ccaggtccat caggggatcg ccgatcgtcg ccatctccca gtccaggatc cccaggaccc
     3961 tcaacggggg attgtcgggc gcgaggacga cgttgtcgaa acggtaatcg ttgtggatga
     4021 cggacacgat gccgctgtcc ccgggcatgg tcttcgtcag ccagtccatg acgtcttcat
     4081 agaccggcgc atcgggggtg cgggcctgac ggtagcgttc gctccagccc tccacctgtc
     4141 gcttcacgta ccccgccggt tttccgaaat tctcgaggcc ggcccggacg taatccacct
     4201 tgtgcagcgc cacatgcacg tcgagcacgt tctcgcacaa ctgccaggtc tgttcggggg
     4261 tgtaggtgag ccccggcggc aggtcgcggc ggaggaggac ccccttgagc cgttccatga
     4321 cgaagaaggg gcagccgatc acggattcgt cttccgtata gaggatcggg gccggacagt
     4381 aggggaaaac gggtttcagg gccttcaaaa tccggtattc ccggcccatg tcgtgggcgc
     4441 tcttggcctt ccggccgaag ggcggacgcc ggaagacgaa ttcccgttcg ccgaccttga
     4501 cgaggtaggt cagattcgaa tagcccccgg gaaactgcgc gaccgtgagt tccccctgca
     4561 gacccgggat ggcgtccttg agaaaggcct ccaccttctt gacgtcgaac tcttctcctg
     4621 cgcgaatcgg tttggtcttg tcgagataat ccatcctgcg cctccgtcgt tgatgtcgtt
     4681 tccagatccc cgaaaatccg atcccccgct gctgcttcgg gatgaagatt ttccccgttc
     4741 ctttagaacc cttcctgcgc acaccggaat cagggcgacg cggcggccgg aaacggcagg
     4801 acatacgcct ccaccagccc cgtgacgaag cgggcatatt cttcgatggt gactttccgg
     4861 cgctgatgct tccagggctt gaggtaccag tcctgcagca gggccttgat cgcggccgcc
     4921 gtgagttcca cattcagggg gcggaagcgc cccgcgcgac agcccgcgga gagaatgtcg
     4981 atgaagagct tctccgtgaa cagctccatt tggatggatt tcttctgttc ttccttggac
     5041 aggttcttcg tctccatata ggagaaatag aaccaggccc gcatcgcctc gctcagatag
     5101 aggtgcgtct gaatggcctg gcagagcttc tcgtggggat cggacacctc cgcaatcgcc
     5161 ccggtcagga tccgaacgac gatccggtac ccctgcgccc ggatgatggc gagcagttcc
     5221 tccttgccgc tgatatagga gtagagcgcc cccatgctca agcccgtctc gcggctcaaa
     5281 tcccggacgc tcatggcctg gaaccccttc tgattgctga tcatcagggt ggcatccagg
     5341 atcttcacca ggttccggac gaccgtggcc tccttcttca cccggacccg gtcgggattt
     5401 tcacggaaaa aatcccggca aagcgcctcc atcgacaggt tgacgagctc ccggaattcg
     5461 gcgaaggtca tcatcgtccg ctcccctctg cacacacgat tcgcggcctg tcccgttcgt
     5521 cggcctcagc ggacgtcgcg tccggcgtag cccttcagga tccgccgggc gacggtcgct
     5581 ttgtggacct cgtcggcgcc gtcgtagatc cgggcggccc gctcatgccg gaagaaaaag
     5641 gcgatgatgg tgtcgtccgt cattcccagc ccgccgtgga cctgcagggc ccggtccacc
     5701 accttctgga ggacgttggc cacgtagaac ttgatgagcg cgatgtcctc ccgggcctcc
     5761 ttgacgccca gggtttcgat cttccaggcg gcgtggaggg tcatcaggcg cgcggcctgg
     5821 atctcggcgg cgcactcggc gatccaggac tggatgatct gcttcgaggc cagggtcttg
     5881 ttgtcgtacg agatgacgcg cttggacgcc cgggcgcaca tcagatcgaa ggcgcggtgg
     5941 cagatcccga gccagcgcat gcagtggtgg atccgacccg gccccagccg ctcctgggcg
     6001 atgacgaacc cccagccctc gggtccgagg aggttctcct gcggcacgcg gcaggactgg
     6061 tagaggatct ccccgtggct gaacaggctg tcgcccgaat ggcccatcac ggggatgttc
     6121 cggaccaggt tgaagcccgg cgtgccggtc gggacgatga tcatgctcgc ccgcatgtgc
     6181 ggcgccgcct cggggttggt cacggccatg acgatcgcga aggccgcccc gtcggcgccc
     6241 gtcgtgtacc acttctggcc gttgatgacg taatcctggc cgtccttgac cgcggtcgta
     6301 tccatcatca ccgggttcga tccggccagc tccacctcgg tcatggaaaa gcagctccgg
     6361 atcttcccct cgatcagggg ccggagccag cgttccttct gcgcacccgt cccgtacttg
     6421 tggaggatct cgatgttccc cgcgtcgggg gcgtggcagt tgaaggtgta atgcccgatg
     6481 ggcgtccgcc ccagggcctc ggagacgagg gcgtgatcca tcaggttcag gcccatgccg
     6541 ccgtactccg gcggatggtt gggggcccac agctccatct gccgcaccat ctgccgcttg
     6601 ccctccatga cgggctccat ctgccggaaa ctcttcgtca gaaactccgg ttccagggga
     6661 atgagttcac gtcccacgaa ttcgttcatc attcccacaa cggcctgcat tttctccgat
     6721 acggtgaaat ccatgatccc ctccctgttc gatgatgggt gatgggttgt ccgacctctg
     6781 tttccgcgtg gtgcgttccg gtcagcggta cgtcatcagc gacgcgtccc gctccaggtc
     6841 gaggtgggcc gtctggttga aggcggccag cgagattcgc tcctcgttgt agacgaattt
     6901 cgtgatcgag gcgttgacga tctgccaggc gatccggagg gtctgctcgt cggagatccc
     6961 cagcgcgagc tgcatggccg ccgaaatggg accgccggag gtaaagatga tcacggtccg
     7021 gcccttgccg tgggccgcca tgatctgttt gagccccccc cagacccgat cgcggaaggt
     7081 ctcccagctg tccaggccct ccttccggtc ctccccggcg atccacttca gcatgatccg
     7141 ctcgaacacg cgctggaagg ccttcctgtc cgtgtagatc tgcttcatgt ccgcatcgag
     7201 cgaccggtcc tcccgcgcca gctcctggac gtgagacagg atgatggacg ccgaattgta
     7261 ctcgttgaag gcctcgtcga cggtcagcgc cggaaaggca aggccgtccc gggcatacag
     7321 atcgagggtc tcccgggccg tgtcctgctg acggaccatc cggcccgcac aggccgcgtc
     7381 gcactgaaac ccgatccgct taaggtactg cgccagcagc cgggactgca gaacgccccg
     7441 ctcggagagc tggtcgtagt tgtggtttcc gaaagaggcc tgaccgtgcc ggatcatgta
     7501 aattgcactc atgaattcga atcccctgat tgtacttata ctacgcccgg aacgacccgt
     7561 caaccacaaa atagagcgag cgctcgtttt agcgccgcac gggaacgccc gcgcccccca
     7621 ccgctcccct cgcggtcgtc aaaggccctg tcggacgaac aaaactgcgg acaacgcctg
     7681 tgctggaaaa ccgggcaagt ctatttgccc ttgacacgtg gacatttgat cattgacagc
     7741 tgtcggcgtt tctcctatgt ttctgtccgt ccctccgccc cgggaatggc gcgagcgccg
     7801 taccgccggt gcggatcccg ttcttcgaaa ccggtccgaa cctcctggag gcatgagaga
     7861 tggagaacat cgcggaacat tttcttcacc ggttctttta tccggccagc atcgccctga
     7921 tcggcgcttc cgaaaatgtc tttcgcccga gctaccacct cgtttccaat ctgctcaagc
     7981 tgggatacgc ggggccgatc taccccgtgc accccaagca gaaggaaatc ctggggatca
     8041 aggcctatcc ggacgtcggc cggatcgaag cggttcccga tctggccgta atcgccgtgt
     8101 cccaggccca gacgccggga cttctcgacg actgcatccg caaggggatc cggcgggtcg
     8161 tcctggtggc cgggggtttt tccgagatcg gcgccgacgg ccgaaggctt caggaagaga
     8221 tggccgggcg ggtccgcgcg agcggggtcc gggccatcgg tcccaatgcg ctcagcccca
     8281 tcaaccccca catcggcctg gccgtcagct tccatgccct ggaccgcctt cactccggcg
     8341 ggctttccct gatcttccag tccggcctct acgaaccccg gctgcgctgg ctgttccann
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggtgttcgac cgcgtcctga
     8521 agcaggtcgg cgcgatccgg gccgagtcga tcgacgaatt cttcgacctc tgccgcgccc
     8581 tggagcgttt cgacggtctg gcgctcgcgg gcaaccgggt cgtgatcgcg acgatgcccg
     8641 gcggggaggc cgtggtcatg acggaccggg tccagcagga gggattgacc atggcccggg
     8701 tgtcggccgg gaccctggaa cggctgcgcc cggtcttccc gccgtgggac atgccggcga
     8761 atcccttcga tctcggggtg accatgcagt tcggcaaccc cgtcacggtg ttcgaaaccc
     8821 tcgtggcatc gctggcggcg gacccgggcg tggacgccgc gcacctccag atccccgacc
     8881 tcctgctggg actgccccgg gaaaccttcg ggatgttttt tcctctgccc gaggccggca
     8941 agcccctggt gctctgggtt gcggggatgg agcccggcga ccatgagacc ctggcctggc
     9001 tggaggatca tcggatcccc gtcttcccgt caccggagaa ggcgatccgg gtgctgaggg
     9061 ccctgcaccg cctgcaggag cgaagccgac gcctccgtcc ggaaaccggc cccccgtccc
     9121 ggcaggacga tcgcccctca tgaacgggga ggcccaccgc tacgaccccc agaccgtgac
     9181 gggaatcttc ctgcagcagg cccggcggta cggggaccgg accctcaaac tcgcgaaacg
     9241 gaacggacgc tggacgccga cgacctggcg ggaggccctg gcggggctgc gggccggcgt
     9301 gatgggcctc gtcgccctgg ggaccgcgaa gggcgacccg gtcgggatca tgagccggac
     9361 ccgggcggag tgggccgacg cggacctcgc catcctctcc gccggcgggg tcacgatcgg
     9421 tctgtatccc acggccagcc ttaaggagac gacccacatc atccgccaca gtgggttgag
     9481 gtgctgtttc gcggaagacg acgcgatcct cgagacgctc ctgtccatcc gcgagacgct
     9541 ggggcagccg gagaccctgg tcgtcttcgc gacccgccgg acgtccctgc cgccgggcgt
     9601 cctgacctgg gatgacttca aggcagaagg gcggatcctg gacgggcggg cgccggagcg
     9661 cttcgaggcg atctgtcgcg ccgtccggcc ggaggatctg gccacgatcg cctacacctc
     9721 cgggacgacc ggcccgccga aaggggccat gatcacccac gccaacctct accacacggt
     9781 cttgaacgcc acgcagatgc atcactacga ggagagcgac ttcggcctcg ccttcctgcc
     9841 gctcacccac atgctccagc ggatgaccgt ctatgccgcg atgcacctgg gcattgtcgg
     9901 ggcctatgcg gaaagcatcg accggctggt ggacaacttc cgggaactca agcccaccgt
     9961 ccaggtcagc gttccccaga tcttcgagcg aatctacaac cgcattcagc agatgctcgc
    10021 cggcggctct cccctgaaag gccggatctt ccgctgggcc gtcgatgtcg gacaccgcgc
    10081 ggcgcgggtc cgccgggacg ggcggcggct gccgcccggg ctcgccctcg cgcacaccct
    10141 cgcccaccgc ctcgtcttcc ggaagatcca cgccgtcttc ggcggacggg tgaaatacct
    10201 cctctgcggc ggggcgccga tgcccctgta tctgctggag ttcttcgagg ccgtgggcct
    10261 gctgatcctg gagggatacg gactgacgga aacggtggcc ccggtcgcgg tcaaccgggc
    10321 ggagcggtac aagctcggca ccgtggggca gctgattccc ggcatggccg caaaaattgg
    10381 agacgacggg gagcttctgc tgaaggggaa aggcctgttt caggggtatt accgggaccc
    10441 ggaggccacc gcggcggcga tcgacggcga gggctggttc cacaccgggg accttgcgac
    10501 cctcgacgcc gagggattca tcacgattac ggaccggaag aaggacctga tcgtcacggc
    10561 gggcggaaag aacgtcgcgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 ncacgagcgc atccgccggt tccggatcct gccccgggac ttcacggagg cggccgggga
    10741 actgaccccg accctgaaga tccgccgccg ggaaatttcc cggcgctatg aggacgtgat
    10801 cgaatccctc tatgccgatc cgcccgccgc gccctgaaaa aaacggcgca cccggcatgc
    10861 cgtgatgcgc cgcgcgtccg ggcgcgatgc ccgccggacc gccgttgtcc tacgccccgg
    10921 gcacccgcac gccgcgctcg cccgcgggga ttccttcgcg ctccagccac tgcccgtaag
    10981 cgtgcatctt ggccagcgcc ttgaccgcct gttccggcgt caggtaaacg acgcccttga
    11041 aggggctgcc ctccacatcc gtaatcgtcc ggctcttttc gtccgtcagg aggtacaccc
    11101 cgaggatcgg tttgccgtac ttctccatga gccggaccat gctctccagg aaaaattttt
    11161 cgtagtcgac gaccttctgc agccgttcct cgatcaaggc cttgttctga ctgggatcca
    11221 ccgcagtcgc ggattcggcc agccattgga ccatgattct ccgtcccatg acccccatgt
    11281 ggatgtacgc gtcgcagccg tcccaccgca tcatctcctc catgaccctg agcgggacgg
    11341 tcaagtcggc ctcgccgacg aggtccaccg ggttggagcg gctccagtac ggcgggagga
    11401 tccggtcgat ccgggcgatg agatccgcgg acaggggggc cagctcgagt ccgttctcca
    11461 cgcacagatc cgccgccaca accccccatc cgccccccag cgtggcgatc ccgacgcgat
    11521 tcccccgggg cagcggcagg gaggaaaagg ccgccgagac gtcgatcagg tccatcggat
    11581 gttcgacgag caccaccccc gcctgacggc aggcggcctc gaacacccgg ttgttggccg
    11641 cgagggcgcc ggtatggctc tccgcggcct tgttgccctc ccgggtgcgc ccccccttga
    11701 ggacgatgac cggcttctgg cgactcaccg cgcgggccgc ctggaaaaac cggcggccgt
    11761 tcttgacgct ctcgatgtac aggagaacgg tccgggtcaa cgcatccacc gcgaaggcgt
    11821 cgagataatc ctcgatggtg atcatggcct cgttgccgga tccgctgaag gcccggatgc
    11881 cgatgccctc tttcctggcg aaggccagca gctgcgtccc cagattgccc gactgggcga
    11941 tgaaggccag cgtacccgga acgggccggg cgtgcatacc ggtgcagaag aattttgcgt
    12001 gcggattgca gatgcccatc gtgttcggtc ccaggatcag caccccggcc tgatgggcgg
    12061 cggcgaccag tttccgttcc agttccttgc cctccggtcc ggtttcgcca aacccggagg
    12121 tgaccagaag catgctcttg acgcacttgg ccttcagttc cggcagaagc gcgatgaccg
    12181 cctccgcggg cacggtgacg acgaccagat ccacgggacc cggaatgtcc ccgagggagg
    12241 ggtacaccgg ccggccggcg atctggccgc ccttcggatt gacgagatnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gccatcccct cgtcgaaggg ggccagacga
    12421 aagacgacat cgtccagggc ctcggtgaag atgcctccca ggccgaacat gaccaccggc
    12481 ccgaactgcg catcccggaa aagaccggcc acgaattccc gccgacccgt cacctgcggc
    12541 tggacgagat agccttcgag gtccggcccg gcgcccttct cgatctcgaa ggccgcctga
    12601 ttgaccgccg ccgcgcttcc gagattcaac ttgacaagcc cccgctccgt tttgtgggtc
    12661 agttttgcgc ccaatccctt gagaacaatc ggataccctg tctgctccgc ctgccgaacc
    12721 gcctccgccg gggttttgac gacccattct tcaatgacgg gcgccctgta agccttgagg
    12781 acggtcttgg actcggcctc ggtcagggtc gtccggccat cggtgataac ctgttgtatc
    12841 aacgtatcta ttttcgtcat ggtgccttcc tctccctttt acgctgagcg gtccttatca
    12901 catttgaccc ttgaacgcaa cgttcccccc tcatccggaa aaagctcgtg atttccaaaa
    12961 gttttgtgtc gagacaaaaa atccttgaca tatataattt taatatatat ccacaaactg
    13021 cgttggcccc ttgctcccgt ttgccgatcg tctgcgggac agggggaaat ccgagcgggg
    13081 cctttcgcgc cccggcacct cgaccgtcgg gagcgatcca tgtccgtccg ctacgcgatc
    13141 ctggggctcc ttcactaccg gcccatgcac ggctacgaga tcaagcggca catcgagcgg
    13201 cacttcggcc acatgtggac gatcaactac ggccagatct atccgaacct gaaatccctg
    13261 gaaagcgagg gcctgatcac cctcacccat gtcagcccgt ccgaaaaggg agggcccaac
    13321 aagaagcttt atgccgtcac gccggaaggt cgcgataact ttgcccagtg gcttgaaagc
    13381 gcggaagact cgcggatgtt tctccgcgat ccctttctca tgcggtttat ctttttcggc
    13441 ttcgggagca aaagccaggc gatcgccacc ctcgaccggc agatcgccca gtacgaggac
    13501 cagtaccggc gtcgcgagca gcacgtcgcg cgctggcagg agcggggagc gtatgtgcgc
    13561 ctcatcgccg aactcggcgt ggaattcaac ggcctgtacg tgcannnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnntctggg cgtcgagtac gtccccgccg acctgacccg
    13741 gccggaaacc ctgccgcccc tgttcgccgg cggcgtggac cgggtcttcc acctgggcgc
    13801 gatctgcaat ttctccaccc cctatgcccg gctccacccc accaacgtcg ccggcgtgga
    13861 gcggttcacg aagctcgcgc aggacgccgg cgtgaaacgc tacgttcatg tcggctcgac
    13921 gagcgtgtac ggcccttacc ggggcgtccc cttccgcgag gacggtcccc gggatcccca
    13981 ggacgactac ggccggagca aacgggacgg cgaggatgtc gtctgggcgc gcatccgcga
    14041 gggcttcccc gccgtcatca cccggccgtg caccgtttac ggcccccgct gcaacgacgg
    14101 tgcgggcaag gcgttttcgc gccccacggc cattgccgcc atccccggcc acggccgtca
    14161 gcgcctctcc aacatccggg ccgaggatgt ggcggccgcc gtggagcacc tctcccacct
    14221 ggacgaggcc atcggccagg cctacaacat cgccgaggac acgaacccga ccgtggaaga
    14281 ggccctgacg ctcgccgccg agacgttcgg gacgaagccc ccgacgctcc gcctcccgct
    14341 ctggctcctc aagatcgtgg ctcggatcga cggcgggctc gctgtccgcc aggggcggat
    14401 ccccgacctc gaatacgacg cggtgcgcta cctgtacgac gattacatcg tggacaacac
    14461 caagctcaag gcaacgggat accgtctccg cttccccgac ttccgggcct cgatggccca
    14521 actgaaggac gtggggaggt agaaaggaca ggcgtggcgc ccttctcaag gcgtcatcga
    14581 tcatcacgaa ccagcggacc ttgaaaagga ggggaactca tgggacaggt ggtcgtcatt
    14641 acagggctgg cggacgggat gggtcgggag gcggcgaagc tcctggccgc ggcgggcgac
    14701 acgatcgccg gtttcgacat ggatgcggcg gggatcgcct cgcttcagac ggaactcgac
    14761 ggcctgtccc cttcgtcggg gaaaacgcat tatctcgttc ccctgaacat tctggatcgc
    14821 ccggggattc tccgttttcg ggacgaggtc ctggcaaaat acaagcgcgt cgatacggtc
    14881 ttgtccaacg tgggcatcgg cttcttcggt cccttcgagg agatcgacct ggaggcggcc
    14941 cggcggtgcc tggagatcaa cgtcatcggg gccgccgcta tctttcaggc cttcctgccc
    15001 gccatgcgcc ggcagggaag cggtaaactc atcgcgatgt cctcgctggt cggtcaggtg
    15061 cctttcccct tcgaatccat ctattcggcc agcaaattcg cgatcgaggg catggtcatg
    15121 tcgctccgct acgaggtcgc cccgttcggc atccgcgtgg ccctgatcga accggcccag
    15181 gtctcgacga ccttcgccgc caagatccac acgctgcccc cggagggatc gccctaccgc
    15241 gagcgggccc gccgcttcat cgcccgcgac gacgaactga tcaagaccgc cccgacgccc
    15301 ctgcaggcgg cccgcaggat cgcggagatc attcatcagg accggccgga cttccacaac
    15361 caggtggact tcatgagcac ctttttcctg tttctcaacc ggtttctgcc ggtccggctg
    15421 cgcgacatga tcctgttgaa tcacatggac atccaatagc cgtctccttt ttacggactg
    15481 atcaaacgac ccggaggtca atggaatggc ggagaagttt atcagtgaac gaaatttgaa
    15541 gttcctgctc aacgaggtct tcgacacccc ctcgctcgtc cggcatcctt attacgcgga
    15601 tcattcaccg gaaacgctcg acatggttct ggatacggcc gtcaggatgg cccgggacac
    15661 cttgaagccc tgtttcaccg agatggaccg gaacccgccc gtgttcgccg acgggaaagt
    15721 ccacgtccac ccgatggttc ggcagatcct caaacagttc ggagagggag gctggatttc
    15781 cgcgatggcc tccaccgaac atggggggca acagctgccc atgatcatcg tcatggccac
    15841 ccatttcatc ttcggcgccg ccaactattc gggaagcatc tatccgagcg cctgcaccgg
    15901 cgccgcccac ctgatcacct ctttcggcac gccggagcag atcgcaacct acgtccccag
    15961 gctgtacgcc ggcgaatggc agggcacgat ggccctcacc gaaccccagg cgggcagttc
    16021 gctcgccgac atcacgacga cggcggaacc caccgcggag ggggattacc gaatcaaggg
    16081 ccagaagatc ttcatctccg cctcggagca tgacggggcg gacaacatcg tcaatcttat
    16141 gcttgccaaa atcaagggcg ccccggcggg catcaagggc atctcgttgt tcatcgtccc
    16201 gaacaaacgc ctcgacggcg cggggaatct cgtcccgaac gacgtcaccg tcacggccac
    16261 gtaccacaag ctcgggtacc ggggaacccc catcaaccag ttgagtctcg gggacaacga
    16321 cgactgccgc ggcgagttgg tgggcctccc ccacaaggga ttatcctaca tgttccagat
    16381 gatgaacgaa gcccggctcc acgtgggcat cggcgccacc gcgatcgcgt cggccgcgta
    16441 ttacgccgcc ctcgagtacg cgaaggcaag gccgcagggg agaaggccga ccgaccggga
    16501 tcccctgtcc ccccagatcc cgatcatcga acacgcggac gtgaagcgga tgctcctctt
    16561 ccagcggtcc gtcgtcgaag gatcactcgc cttgctgctg cagtgcagca agtatgcaga
    16621 tttaaacctg tccgccccgg aagaggagaa ggagcgcgcg gcgctgcttc tggacctgtt
    16681 gacgccggtg gccaaaacct atcccgcgga aatgggcatc ctctcgacga gccagggatt
    16741 gcaatgcctg ggcggatacg ggtattgcga ggagtttccc ctcgaacagc atttccgcga
    16801 cgcacggatc catccgatcc acgaaggcac aaccgcgatt cacggtcttc tgcttctcgc
    16861 ccggaacgtc acgatgaaaa acggtcgggc cttcaccctt ttcctggagg aggtcggccg
    16921 ggtcatcgcc gcaaccgaca cggacgaggg cctcaaaccc tacggccgtc agttggccga
    16981 ggccctcgaa cggctcaaga acgtgacaaa tcatctcctg gggctggcga tgcaggggtc
    17041 gatcgatctt ttcctggcgg atgccacgct ttacatcgag tgcttcggca tcatcgccgt
    17101 ggcctggcaa tggctcgtgc aggcacaggt tgcccggacg gccctggcga agggaccggc
    17161 cgcggaagag gccaacttct atcaggggaa gatgtacgcc ttccggtatt tcttcggcta
    17221 tgagctcccc aaaatcgcgg ggctggcaac ccggctgctc aacagcgacg gcctgaccgt
    17281 cgagatgaag gaagatttct ttaacgattg aggaggttcc catgtcccaa ccgacccccc
    17341 ccgtttatga ggtcccgaag acgcgcatcg ggcgcctgcg ggaacggatc atccacgccc
    17401 cgcaggaggt ctgcatcgag cgggcgcgct acctgacgga ggcgatgtcc cgccactggg
    17461 accgtcctcc cctgacccgg atgagcctag ccctggaaca catcctcgcg aacatctcgg
    17521 tcgtcatccg ggacgacgag ctcatcgtcg gctcccggac gagcaaactc aagggcgcgc
    17581 ccctcttccc ggaaaacaag agccgctgga tcgccgggga cgtggacaac ttcgaccggc
    17641 gcgtcctcca gcgggccctc atcacggagg ccgaaaagcg ggaactcgag acggccatcc
    17701 tccccttctg ggagggcagg accgtggagg acctcttcga ggcccgcctt cccgaagaca
    17761 tcgccgccga catggacaag tacatcttca cgatgatgct cgagatcacc tacggcatcg
    17821 gccacttcac gatgaaccac cgccggatcc tccgcgaggg tctgggggga acgatcgcca
    17881 ccgcagccgc gcgccgcgag gctctggcgc cggccgaaca ggccggggaa aagggtcttt
    17941 tctacgacgc cgtcatccgt tcactccggg cggccgtcac cttcgccaac cgctacgcgg
    18001 ccctggcgcg gaccctggcg gcggaggccg cctcgccgaa gcgccgggcg gaactcctgg
    18061 aaatcgccgg gatttgcgaa cgcgtccccg aacaccccgc gcggacgttc gccgaagcgg
    18121 tccagtgcgt ctatttcatc cacctgatct cccagatcga atccggcggc aactccatct
    18181 ccctcggccg gatcgatcag atcctttatc cctactatgc ggcggacctc gcggccggcc
    18241 ggatcacgcc ggaggcggcc cgcgaacggc tcgcgcttct gttcatcaag accaatgaga
    18301 tctggaacgt cctggaggag gccttcatcc ccggcggcga agggacggag ggcaagacga
    18361 cccagaacgt caccgtcggc gggatggacg cccggggcga ggacgcgacg aacgccttga
    18421 gcgagatcgg cctcgacgcc tttgcggaca tccggacggt ccagcccaac ttcggcatcc
    18481 gcctgtgcgg caagaccccg ctcgcgttct tccaaaaagc ggtccggtac gccaaggacg
    18541 ccgttccgct ccacctgttc aacgacgacg ccatcgtgcc ggccctcgtc cggtcgggac
    18601 acacgctgga ggacgcccgc gactacggcg tcgtggggtg cctcgagccc aacgcccagg
    18661 gaaagtcctt ctgctccacc ttcgccgtcc agttcaacgg gatcaagtgc ctggagttcg
    18721 ccctgtcgaa cggcacggac aacatcttcg gctacgcctc gggcatcgag acgggcgacc
    18781 cggcgacctt cacgaccttc gacgaggtct ggcgggccta cgaccggcag atgacccact
    18841 tcatcggcca gatgacccgc ggcatggccg tcctcgacga ggccatcgcc gagcgggtgc
    18901 cctccccctt cgcctcggcc atgatcgacg gcccgctgga aaagggtctg gacctgaccc
    18961 ggggcggggc ggtctacaac gccaccggcg tccagctcat gggtttcgcc aacgtggccg
    19021 acagcctccg cgccctggag aaggccgttt tcacggaccg ggtctgcacg ctgtcggaac
    19081 tcgtcgggtg gctctcggac gactggatgg acaccgaaga caaacgcctg tattttctcg
    19141 acaagattcc gaagtacggc aacgatgacg acgccgcgga cgcgatggcc gcccgggtca
    19201 tggcgcactt ctgcgacacc gtctccgccc gtcggaactt ccggggcggg tccttctggc
    19261 ccgggatctt ttccgtgggc ttccatctgg cattcggctc cttcacgggc gcgacaccgg
    19321 atggccgcag cgccggcgat atcctcggca acggcgtcac gcccgcaaac ggccgggcgc
    19381 tgaacggccc gacggcggtc ttcaactccg tgacgaagct cccgctcgca gaggcgacga
    19441 acggcgtcaa cctgaacatg cgcttttcag gccggcgcct caagccggag atcctcctcg
    19501 ccctgatccg ggggtatttc cggcggggcg ggatgcaggt ccagttcaac atgatcgatt
    19561 ccgacgttct cagggacgcc caggcccatc ccgatcagta ccgggacctc gtcgtccgga
    19621 tcagcggcta ttccgtgacc ttcgtggatc tgagcgacac ggcccaggac gagattatca
    19681 accggatggc gttcgcgctc taggaaacaa gaaacaggca ctggggacca ggcggcgggc
    19741 aagcggcagg gggcaacagg aggcgcctgg ccccctgccc gcaaaaaacc gcagcaggac
    19801 atcgaagggg ttcgcgggtg tatacggcgt tcaggctcaa gaccggacgc caaacgtcca
    19861 aaaaaggggg ggagcatgac atcggagcgg aagattttct acgggtggtg gatcgtcggc
    19921 ggcgcctttt ttctgaactt cgccggcatc ggcatcatca tgaacaccat gggcgtcttc
    19981 atcaagcccg tcacggaatc cctcggtttc acccggggcg gcttcacgct gtacttcacg
    20041 atcgccgcgc tctccatgat ggtcatggcg ccggtcatgg gaaagctcct ggaacgttac
    20101 aacatccgcc tcatcatgac ggtctgcacc gcgatgatgg ccacgagctt cgccctgttc
    20161 tcccaatgcc ggacgctgac gcagttttac atcgtcgcgg tgttcctggg gatcgggagc
    20221 gccgggtcgc acatcatccc cgtctccatg atgatcacga actggttcat cgacaagcgg
    20281 gggctggcca tggggatcgt cttcgccgcg acaggcgtcg gcgggatgat cttcaacccc
    20341 ctggcaaact ggatcatcct gaatcacagt tggcaggccg ccttcctgac cttcggtctg
    20401 gtgatcggcc tcgcctccat cccgacggcc ctcttcatcg tgcgcgccaa accggccgac
    20461 atgggtctgc ttccctacgg gggggaggcg gcgctcgccc gtcagtccgc cgccgaacag
    20521 gggggcctga cggcgaccaa ggcgttccgc acgggcgcct tctggctgct cgccctgatc
    20581 atccttctca tcgccgtcgc caacatgggc gtcctgcacc acatcgtgcc ctacctgacc
    20641 gacctgggtt tttcctccac gaccgcaacg acgctcatgt ccctgcacat ggccatgctc
    20701 atcttcggca aggttctggc gggcagcctc gccgaccgcc tgggtctgct caaaagctac
    20761 ctcatcctga tggtcggcct gatggtctcc atcgcgctcc tttacgggtc gcaatggatg
    20821 tgggtggcgg tcgtcttcaa tatcctgttc ggtttctcga tcgccgtacg gacggtcctg
    20881 ccgccgctca tgaccgcccg ggtcctgggc cagaagcact ttgccgtcat ctacggattc
    20941 ctgaacatct tcacgaccct gggcacagcc gtgggcgtgc ccctgtccgg cttcatctac
    21001 gatgcgacga aaagctacta cctggcgttt gccctgtaca tcgggctttg cctcattaca
    21061 acggcggcgg gcattgccgt cctgacccgg agcggcaaga agtgaccggc aacaacccgg
    21121 cgtccgggtc aagaccggcg ggacaaaccg gaggcatgac cgcaagcggg ggttttgccg
    21181 tcatttgacg gtttcacatc acatcgatag gggttgaagg catgagcaag gtaatgacga
    21241 tgcgggaggc gatcgccgac cacgtggcgg atggcgattt cgtctatatc ggcggctacg
    21301 tctgccggac gccgttcgcg gcggtcc
//
LOCUS       DAOOLJ010000029        20200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2638_length_20200_cov_18.362770, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000029 DAOOLJ010000000
VERSION     DAOOLJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 20200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20200
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_20200_cov_18.362770"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..484
                     /locus_tag="PLW16_06335"
     CDS             212..484
                     /locus_tag="PLW16_06335"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPB70557.1"
                     /translation="MEAEDWGVVTAASGLTNARIITGRLETEGIPTRLRYEAAGAIYA
                     ITIDGLGEVKILVPARELARAREILERSYDEADLHGNGAGPEPDGL"
     gene            complement(479..1492)
                     /gene="amrS"
                     /locus_tag="PLW16_06340"
     CDS             complement(479..1492)
                     /gene="amrS"
                     /locus_tag="PLW16_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPB70558.1"
                     /translation="MNREALLYERLPDDEVRCGLCAHRCRIRPGRRGICGVRENRAGI
                     LHSLVYGNLIAENLDPIEKKPLFHVYPGSRTFSVATVGCNFRCTFCQNHDISQMPRET
                     GDVLGRPVAPEQVVLSSAETGSRTIAYTYTEPTVFFEYALDIAKLAHARGIGNVFVTN
                     GYMTAEAVATAAPYLTAANVDLKSFRDEFYREQCGARLQPVLDSLRALKAAGVWVEVT
                     TLLIPGLNDGEEELRDIARFIHGLGPETPWHVSRFHPQFRMRTTPRTPPRAIRLATDV
                     GRAEGLRYVYSGNLPGDEGEKTFCHGCGQLLIDRFGYTIRGIDLDEARCPRCRTPLAG
                     VFS"
     gene            complement(1489..2259)
                     /locus_tag="PLW16_06345"
     CDS             complement(1489..2259)
                     /locus_tag="PLW16_06345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB70559.1"
                     /translation="MIFDEALSRSYDDWFQTPSGRYIDAREKAFILSLMSPRPGERLL
                     DVGCGTGEHLRMFREKGCDVTGLDASPFMIAQAREKLGSRAELHEGRAEDLPFSDNEF
                     DHVVLITALEFTEDPARAVSEAIRVCRDRVFLGLLNPYSLLAVHRGLEGRLRDTVYNR
                     ARLFSLRKVSGMIRDRLGSTPLAWGSVILFPASWYPAAGMLEERLPAVKNPFGAFMGL
                     SFPMRFRYRTVQDPIRDPFKMKANQRNPVQGAVREMER"
     gene            complement(2300..3346)
                     /gene="moaA"
                     /locus_tag="PLW16_06350"
     CDS             complement(2300..3346)
                     /gene="moaA"
                     /locus_tag="PLW16_06350"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPB70560.1"
                     /translation="MKTLNAARENGAGGGGMRDRFNRDINYLRVSVTDRCNLRCRYCM
                     PKAGLSLIGHDDILRYEEMIRIVRVAVRLGVGKVRLTGGEPLVRRGLAEFIASLRKIE
                     GLHDIGLTTNGILLEKMAPALYAAGLRRINVSLDSLDPEKYRHITRGGDLEAVIRGIR
                     KARECAFDPIKINVVAIKGFNDSELFDFAALTLERPYQIRFIELMPLGEAGTENNGNF
                     LSNDVIRQRIGERYALEPVNDGKNPEAGPAQVYRAAGGQGEIGFISPVTHHFCETCNR
                     LRLTADGCLRACLLRDDEVDLKRPLRDGCSDADLEALIRGAIAKKPPRHELSRGQAQL
                     KKCARQRDMSAIGG"
     gene            3643..4125
                     /gene="greA"
                     /locus_tag="PLW16_06355"
     CDS             3643..4125
                     /gene="greA"
                     /locus_tag="PLW16_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPB70561.1"
                     /translation="MEKMPITRAGYEKLKKDLEAVKNVDIPQNIRDIEIARGHGDLSE
                     NAEYTAAKERQSFLHGKMQELETNLACSRVIDLAGMTYEKVVFGATVTIADIDSGEET
                     TYQLVGPFESDINANRISVTSPIGRALIGKVVGEEVTVRAPGGIRKFEVADIAAPADA
                     "
     gene            complement(4408..4815)
                     /locus_tag="PLW16_06360"
     CDS             complement(4408..4815)
                     /locus_tag="PLW16_06360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPB70562.1"
                     /translation="MADDLILEIVTPEKMVFSEKVEEVTLPGTEGEFGVLRGHASLLS
                     SIEIGEMNMTREGKKSFYALNTGYVEVTSGKVTVLVESAERSDMIDKDRAQRAKDLAE
                     NNLARIAKEDADFEVMRAALLRAITRLKVAERS"
     gene            complement(4830..6236)
                     /gene="atpD"
                     /locus_tag="PLW16_06365"
     CDS             complement(4830..6236)
                     /gene="atpD"
                     /locus_tag="PLW16_06365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB70563.1"
                     /translation="MNIGKIVQVIGPVVDVAFEEGKLPSIMNGITITNAAINDEPDNL
                     IVEVAQHLGDNVVRCIAMDITDGLVRGMPAKDMGMPITVPVGPECLGRILNVVGRPVD
                     GLGPIDVKHTAPIHREAPSFLEQDTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMIHNIAMHHGGISVFAGVGERTREGNDLYLEMKQSGVIKQAALIYGQMTEP
                     PGARARVSLTALAAAEYFRDEEGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATDLGELQERITSTTKGSITAVQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAE
                     LGIYPAVDPLDSTSRILDPNVLGLEHYQVARQVQVTLQKYKDLQDIIAILGMDELSEE
                     DKLTVSRARKIQRFLSQPFFVAAQFTGTDGKFVSVADTVRGFKEILEGKHDDLPEQAF
                     YMVGGIEEVIEKAKRLSE"
     gene            complement(6294..7169)
                     /gene="atpG"
                     /locus_tag="PLW16_06370"
     CDS             complement(6294..7169)
                     /gene="atpG"
                     /locus_tag="PLW16_06370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPB70564.1"
                     /translation="MAALKDIKRKIGAVHKTKQITKAMNMVAASKFKSAQNRMESFRP
                     YAGKFMDVLNSLALRVDAKNHPLLSVRDPKKIRVICMTSDRGLCGGFNTNLIKATERF
                     LKDKLAQGKDVTLVPVGRKGRDYFRKKAATMNERTDVFGKFDMSLAVKISNDVIPPFI
                     GEEYDELYLIYNEFASVSTQKPVVLRLFPLPSIGQEEDIDPEKRVEYIYEPSDEVLLD
                     KLLPMYVHVLLFRALLETSAGENGARMAAMDNATRNCDELIQSLTLQYNKARQSAITA
                     ELMDIVGGTEALAKG"
     gene            complement(7233..8747)
                     /gene="atpA"
                     /locus_tag="PLW16_06375"
     CDS             complement(7233..8747)
                     /gene="atpA"
                     /locus_tag="PLW16_06375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPB70565.1"
                     /translation="METIRAEEISQIISKQIKEYEKKLDISETGTVLSVGDGIARVYG
                     VENAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTHIKEGDIVKRTGRIAQVPVGE
                     ALLGRVIDATGAPIDGKGPIETTEFRRIEMIAPGVIQRQPVNQPLYTGLKAVDAMTPI
                     GRGQRELVIGDRQIGKTAICIDAIIRQKDTGVKCIYVAIGQKKSTVAQVVENLRKHGA
                     MPYTCVVSACASDPATLQYISAFAGCSIGEYFRDRKQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAFPGDIFYNHSRLLERAARVSEELGGGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQVYLEPGLFYSGVRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQFRE
                     LAAFAQFGSDLDKATQAQLDRGVRLVELLKQPQFQPMSLGEEVTVLFSGTRGFLDKYG
                     VDKLGEYEPQLLSFVKSKYPEVLKEIDEKQVISPDLEKKMKDVLTEFDTVFVAE"
     gene            complement(8751..9296)
                     /gene="atpH"
                     /locus_tag="PLW16_06380"
     CDS             complement(8751..9296)
                     /gene="atpH"
                     /locus_tag="PLW16_06380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPB70566.1"
                     /translation="MISSNIAKRYAKAFFEIAGEERKYEPYYKELRQFSSLLEMNKNL
                     REFLDNPIFDQADKKAVVTSLLEKTDISPVTANFLKLLVDKRRISVLSEIEDCYRELM
                     DAVLKNVRVQVKTAFPLSPQMVQSLRSGLESMTGKHVEMAVMDDPSLVGGIVVRVGDT
                     IYDGSIRTQLNKIRNLLGEEI"
     gene            complement(9293..9910)
                     /gene="atpF"
                     /locus_tag="PLW16_06385"
     CDS             complement(9293..9910)
                     /gene="atpF"
                     /locus_tag="PLW16_06385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPB70567.1"
                     /translation="MRGWLTARRHGRLVSRSLLTAIGWVLLTAAVVYASGGGAEGGHD
                     KWAEWKDFGWRMLNFAVLAWFLWWMLAKKVKEFFVGRKGEIGTALDDARRAKEEAEAK
                     FRDYDARLQKATGEIDGITEMIRSQGEAEKARIVEDARKQAEKMKEDAQARMEQEFNK
                     ASSQLRLEAVKLSVELAEEILKKQITPQDHETMVRDYIDKVVKQN"
     gene            complement(9907..10338)
                     /locus_tag="PLW16_06390"
     CDS             complement(9907..10338)
                     /locus_tag="PLW16_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPB70568.1"
                     /translation="MTIGLDYTFLIQIINFLALIFILNLLLYKPILAIINRRKKQLDD
                     SELEIGRLNETVEQKMAAYEEKLRQAKAGALEKKQEITQEGAVKAKGIIDAVRGEIPG
                     LVETFQARMNQEVSQARRILHDQSRRISVEIAEKVLGRSVQ"
     gene            complement(10763..11872)
                     /gene="rodA"
                     /locus_tag="PLW16_06395"
     CDS             complement(10763..11872)
                     /gene="rodA"
                     /locus_tag="PLW16_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPB70569.1"
                     /translation="MKIDRRLLLNFDWTLLVLVLVISGIGLMNIFSTGYSLSGLRQTP
                     LYLKQLQWIMIGLAAMVIAFSVDYRLIHRYAYVIYGVGVVLLVVVALYGYTTRGSQRW
                     IALGGFMLQPSEMMKLALILALARYFDRHRSLQGYTTRELVMPLILVLVPFLLILRQP
                     DLGTGLILLIIFIAMVVFSGVRWRSVLWVGAGGLALVPLAWFFLHDYQKARLLTFFNP
                     ERDPLGAGYHIIQSMIAIGSGGLFGKGFLKGTQTQLKFLPEQQTDFVFSVFAEEWGLA
                     GGLVLLILFLLLILWGFKIAQRARDLSGTLIAFGVTVMIFSEVFINIGMVTGILPVVG
                     IPLPFFSYGGSSMVVLLTGVGLMLNISTRRFILHS"
     gene            complement(11869..13791)
                     /gene="mrdA"
                     /locus_tag="PLW16_06400"
     CDS             complement(11869..13791)
                     /gene="mrdA"
                     /locus_tag="PLW16_06400"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPB70570.1"
                     /translation="MSMNSNPLNSHEPGEFRRRFQALFVVVFIALSILFVRMWYLQVI
                     KGEELRQRSENNSVRMRKLPALRGLILDTNGTVLVDNKTSFDLVFIPDRSRDIRQTVD
                     KFAALCRKRSLAFAPDVPAKGRQRTFQPLRLERNVAWEKLALAETHALELPGLITEVS
                     PVRQYLAGEVMAHVIGYIGEIDAEELERNPAGAYAMGDMVGKFGIEKYLDPYLRGTNG
                     FEQVEVNAVGKGVRSLGRIDPVSGYNVVLNIDSYLQKTAWDAMAGRTGAVVVMDPRDG
                     AILAMLSTPSFDPNLFTGKVSLADWRKLADNRLRPMENRAASGQYPPGSTYKPVVAAA
                     ALEEKLITPETTVYCNGSFVMGNRTFRCWQRKGHGRVNLHRALVESCDVFFYEVGRRV
                     GVDTLARYARELGLGMPTGVDLPREKAGLIPTREWKLKRFRQPWIPGETISIAIGQGY
                     DLVTPIQLLNAYAALANGGTVWQPRLVKRIETQDGKPVREFPPVRRSVASISAETLKR
                     INRGLWGVVNEPRGTGYVLRRREADVSGKTGTAQAVGLPQDEKARRLKLAVARGDHAL
                     FVCFAPDKAPEIAIAVIAEHGGHGGATAAPVARRIIDAYFARKNAPPAPPRSVVANAA
                     PAAAPRGANAPPEPNP"
     gene            complement(13842..14345)
                     /locus_tag="PLW16_06405"
     CDS             complement(13842..14345)
                     /locus_tag="PLW16_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70571.1"
                     /translation="MLFYLAAPFLAFCLVVFQQGFLNLFFFQQLSVEVSLLLAIYAGF
                     RMDLARGAVLSVLMGFFVDCLTGSITGLYMTAYALVFLLSFTLSARVYVKQGTLVMTY
                     VIVCTLLEGLLVVGLTALIHGVPLEGRLRVFVLQALVLGVLSPAVFPVFDRVRSFLEV
                     DEPRPMP"
     gene            complement(14351..15160)
                     /gene="mreC"
                     /locus_tag="PLW16_06410"
     CDS             complement(14351..15160)
                     /gene="mreC"
                     /locus_tag="PLW16_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPB70572.1"
                     /translation="MMFFQRYRTVIVVTLLAVLCLVVISISIRHPGRTGVFRQIVLEC
                     AAPVEGLLGRPIHAAAKAWRRYLFLFGLEAEVGRLRRQNAILTRRLVQYQEGYLESRR
                     LRKLLDLKEHDGYRGTTARVTGYVRSATARTLVIDRGSAHGIKPGQAVIVAEGVVGRV
                     MEASWHAARVLLIVDESSNVDAVLQPSRVQGILQGRGGRGCQLKYVGKIAPVRAGDAV
                     VTSGMADIFPKGLLLGVVREVERTTAMFQKIEVTPAADFSRLEEVLVLTGR"
     gene            complement(15266..16303)
                     /locus_tag="PLW16_06415"
     CDS             complement(15266..16303)
                     /locus_tag="PLW16_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB70573.1"
                     /translation="MLFDFILGKFSNDLAIDLGTANTLVYVKGKGIVLSEPSVVAVHK
                     DARGQKKVLAVGSEAKKMLGRTPGNIVAIRPMREGVIADFDVTEAMLRHFILSVHNRR
                     TLVRPRIIVSIPSGITQVERRAVRETVESAGAREIYLIEEPMAAAIGAGLPIKEPISS
                     MVVDIGGGTTEVAVISLAGVVYAKSVRIAGDRIDEEIVQYLKRKYSLLVGERTGEMIK
                     TTIGCAYPDQELRKVDVKGRDLISGIPKTIEIDSEEVREAIAEPVSMIVEAIKDALEN
                     APPELAGDIVDRGIVLTGGGALLRNLDVLLREETGLPIIVADDPLSCVAKGAGMALDE
                     LDVLKEITVQL"
     gene            16484..18082
                     /locus_tag="PLW16_06420"
     CDS             16484..18082
                     /locus_tag="PLW16_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPB70574.1"
                     /translation="MLELMRKHARNWIMKVLLGIIILVFIFYFGSMGGQQQAETVAKI
                     DGKILAYVDLQKEYQNLTELYRQRFGGNLPEELLDSLDLKQKALDTLIDQAVIMAKAK
                     DMGIEVTDEEVRASIMAYPAFQRDGVFNDSLYRRMLQYNKVTPEEFEAMQKKGLTTVK
                     LETLIQEGIKVSDREVYDLYRAQTEKISVSYVQLAPADFAGRVRPTRADLEDYLRVHG
                     DQFRVPEQLQVKVLAFRGADYATQVKVTDDEVAEAYESRKETFTRGGKTAPLAAVREA
                     IAAELRHVGGMDRASDEAKQAHDTIYQEENFEGYAGQNRLKLATTDFFPLNRPPAELA
                     GIKDFAKTAFALRKGDVSGVLSDERGYYVVKVEARKPAYVPELKAIEANVARAYTAEE
                     AKKLCRREAEEMLGRLKKGEGFAALAGAKGLPVGKTGFFIPGTAVPKLGLSQELAEAL
                     FQISEKKPYPDSVVEMGERLVLIRFKARQAASDAQFAGQKDALRGALLTAKRNEAVRS
                     WLEETRKAMIKSGRLEITKDLKDL"
     gene            complement(18120..18455)
                     /locus_tag="PLW16_06425"
     CDS             complement(18120..18455)
                     /locus_tag="PLW16_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70575.1"
                     /translation="METARAFDDAAEALLEDILKKQPIRDFLTASETAAILGITKQAL
                     HKNRRIRHGFIYRTSLGGLAVYLKKSVELYKKKGDGRFPLSELTSDLGEKPASTRRVV
                     DSVYRVHEP"
     gene            complement(19098..19409)
                     /locus_tag="PLW16_06430"
     CDS             complement(19098..19409)
                     /locus_tag="PLW16_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HPB70576.1"
                     /translation="MKRTSAYKEELLTALRDPEEAAAYLNAALEDANQEVFLLALKDV
                     IAARGGISRFADKVDLNRVSLYRTLSKRGNPELRTLNRMLGELGFSLSIKTHAPVGSN
                     A"
     gene            complement(19635..20060)
                     /locus_tag="PLW16_06435"
     CDS             complement(19635..20060)
                     /locus_tag="PLW16_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HPB70577.1"
                     /translation="MVPAKVRMKRVVLDTNVLVSALLFGGRLDALIGRWKSGDLIPLF
                     SRATFAEFRRVLAYPRFGLTGPEINALIEEEILPWCEVVETGEEIRGVCRDPGDDIFL
                     SCAVAAGADAIVSGDRDLLDLGAFRDIPILSVADFLKIK"
BASE COUNT         3710 a         6553 c         6267 g         3670 t
ORIGIN      
        1 gaaaaataca acaaaatgtg gtcggtcgaa atcaccacca caacatcttg tgtccgtcac
       61 gaattctctt ggccgcttcc cggagccatg ttaaatatgt ttcggctttt gaaaatcgtc
      121 gccctttggc gtgccggaca gacggatgag gccacaacga tctcgcgtcg cgaatgtccg
      181 cgacgggaac gaaacgggag ggtcggaatc tatggaggcg gaggactggg gggtcgtcac
      241 cgcggcctcg ggcttgacga acgcccgcat catcacgggc cggctcgaaa cggagggcat
      301 cccgacccgc ctgcggtatg aggcggccgg cgccatctac gccatcacca tcgacggtct
      361 cggagaggtg aagatcctcg tcccggccag ggagctggcg cgcgcccggg agatcctgga
      421 gcggtcgtac gacgaggcgg acctgcacgg gaatggggcc ggaccggagc cggacggcct
      481 atgaaaagac gcccgccagc ggggtccggc agcgcgggca gcgcgcctcg tccagatcga
      541 tgccgcggat ggtgtagccg aagcgatcga tcaggagctg tccgcagccg tgacagaagg
      601 ttttctcccc ttcgtctccg ggaaggttgc cgctgtaaac gtaccggagg ccctccgccc
      661 gaccgacgtc cgtcgcgaga cggatggccc gcggcggcgt ccggggcgtg gtccgcatcc
      721 ggaactgcgg gtgaaaccgg ctgacgtgcc agggcgtctc cggcccgagg ccgtggatga
      781 agcgggcgat gtcgcgaagc tcctcctcgc cgtcattgag ccccggaatc aggagggtcg
      841 tcacctcgac ccacacgccc gccgccttca gcgcccgaag gctatccaga accggctgga
      901 gccgggcgcc gcactgctcc cggtaaaatt cgtcccggaa cgatttcagg tccacgttgg
      961 cggccgtcag atagggggcg gccgtcgcga cggcctccgc cgtcatgtag ccgttcgtca
     1021 cgaagacatt cccgatgccc cgcgcatggg ccagcttcgc gatgtccagg gcatactcga
     1081 agaacaccgt cggttccgtg taggtgtagg caatcgtccg gctgccggtc tcggcggacg
     1141 agagcacgac ctgctccggg gcaaccggcc ggcccaggac gtcgcccgtt tcccgcggca
     1201 tctgcgagat gtcgtggttc tgacagaacg tgcagcggaa gttgcacccc accgtcgcca
     1261 cggaaaaggt acgggagccg ggataaacgt ggaagagcgg cttcttttcg atgggatcga
     1321 ggttttcggc gatcaggttg ccgtagacca gcgagtgcag gatgccggcc cggttctccc
     1381 ggaccccgca gatgcctcgc cggccggggc ggatccggca gcggtgggcg cacaggccgc
     1441 accggacctc gtcatccggc agtcgttcat acagcagggc ctcccggttc atcgttccat
     1501 ctcccgaacc gccccttgca cggggttgcg ctgatttgcc ttcattttga aagggtcccg
     1561 gatcgggtcc tgaaccgtcc gatagcggaa acgcatgggg aaagacaggc ccatgaaggc
     1621 cccgaaggga ttcttcacgg ccgggagccg ctcttccagc atcccggccg cggggtacca
     1681 cgacgcgggg aagaggatga cgcttcccca cgcaagcggc gtcgatccca gtcgatcgcg
     1741 gatcatcccc gacacttttc gcaagctgaa gaggcgcgcc cggttataga cggtgtcgcg
     1801 gagtcggccc tccaggccgc gatgcacggc gagcagcgaa tacgggttga ggagtcccaa
     1861 gaagacgcga tcgcggcaga cgcggatggc ctcagagacg gcgcgggcgg gatcttcggt
     1921 aaattccagg gcggtgatca gcacgacgtg atcgaattcg ttgtccgaga aggggaggtc
     1981 ttccgcgcgc ccctcgtgga gctccgcccg gctgcccagc ttttcccgcg cctgtgcgat
     2041 catgaacggc gaggcatcga ggccggtgac gtcgcacccc ttctcgcgga acatccgcag
     2101 gtgctccccc gtgccgcacc cgacgtccag gaggcgctcc cccggccgcg gcgacatcag
     2161 cgacaggatg aaggcctttt cccgggcgtc gatgtagcgt ccggacgggg tctgaaacca
     2221 gtcgtcgtaa gatcgggaca gcgcctcatc gaagatcatc tgcgtccccc cttctccgcc
     2281 ggaccctaaa gcccgcggtc taaccgccga tggccgacat gtcgcgctgc cgcgcgcatt
     2341 ttttcagttg cgcctgcccg cgggacagct cgtgccgcgg gggctttttc gcgatcgccc
     2401 cccggatcaa cgcctccagg tccgcgtcgc tgcatccgtc ccgcaggggg cgcttcagat
     2461 cgacctcgtc gtccctcagg agacaggccc ggaggcagcc gtccgccgtg aggcgaagac
     2521 ggttgcaggt ctcgcagaaa tggtgggtga cggggctgat gaagccgatc tcgccctgcc
     2581 ccccggcggc ccggtagacc tgggccggcc cggcctccgg gtttttcccg tcgttcacgg
     2641 gttcgagggc gtaccgttcg ccgatccgtt gccggatgac gtcattggac aggaagttgc
     2701 cgttattttc cgtcccggcc tcccccaggg gcatcagctc gatgaagcga atctgatacg
     2761 gccgctccag ggtcagcgcc gcaaaatcga acagctcgct gtcgttgaac cccttgatcg
     2821 ccaccacgtt gatcttgatc ggatcgaacg cgcattcccg ggccttccgg atgccccgga
     2881 tgacggcctc caggtcgcct ccacgcgtga tgtggcggta cttttccgga tcgagcgaat
     2941 cgaggctgac gttgatgcgc ctcaagcccg ccgcgtagag cgccggcgcc attttttcga
     3001 gcaggatgcc gttggtcgtc aacccgatgt cgtgcagccc ctcgatcttc cggaggctgg
     3061 cgatgaattc cgccaggccg cgccgcacga gcggctcccc ccccgtgagg cggaccttgc
     3121 ccacgcccag ccggaccgcg acccggacga tgcggatcat ctcctcgtag cgcaggatgt
     3181 cgtcgtgacc gatgagcgac agccccgcct tgggcatgca gtaccggcag cgcaggttgc
     3241 accggtccgt cacggaaacc cgcagatagt tgatgtccct gttgaaccgg tcccgcatcc
     3301 cgcctccgcc cgccccgttt tcgcgcgcgg cgttaagtgt cttcaatccc gctggttttc
     3361 taacacagtt ggccccgcct ggcaagcggg gaagcgccgg cgtaaaccgg cgcgcagcgg
     3421 catcaggcca tccgcggacg aggaccggcc ggcggggaac ccgctcgggc aatacggcgc
     3481 acaacagtca tgggcaaacc ggcggccgat ggcggccggg caggcgcgac acggccctcg
     3541 ggcgaatggc gcttgacagg gaaccgtttg tcgtctaaag ctaggccgga tcaacggaac
     3601 gcggcgataa cgcaggcgcc gcgccgcgga ggatgagacc ccatggagaa aatgcccatt
     3661 acccgggccg gctacgaaaa gctgaaaaag gacctggaag ccgtcaagaa cgtggacatc
     3721 ccgcagaaca tccgggacat cgagatcgcc cgcggccacg gcgatctctc cgagaacgcc
     3781 gaatacaccg ccgcgaagga gcggcagtcc ttcctgcacg gcaagatgca ggagctggaa
     3841 accaacctcg cctgctcacg ggtgatcgat ctggcgggca tgacgtacga gaaggtggtc
     3901 ttcggcgcca ccgtgaccat cgccgatatc gactccggag aagagaccac ctaccagctc
     3961 gtcggaccct tcgagtcgga catcaacgcc aaccggatct cggtcacctc gcccatcggc
     4021 cgggcgctga tcggcaaggt cgtcggcgaa gaggtgacgg tccgggcgcc gggcggcatc
     4081 cgaaagttcg aggtggcgga catcgccgca cccgcggacg cctgacgcct cgccaaaccc
     4141 gaatcccccg aagaaccggg ccgcaacccc tgacgcattt ttaatgcaga tcgcgggacg
     4201 cctgaaccgt ccggatcccg gggcggccgg ggcgtgaagg cggccggaag gaacgcccga
     4261 accggttccg gatgcagccc ccccgaagcg gccggggaca aggccgggtc gcgcagacgg
     4321 cggcctcccc gattgaaggg acaaaaaaac cgggaatccc gcaccaggat tcccggtcgt
     4381 ttttttttaa gtctgtcgga tcccggctca ggaccgttcg gcgaccttca ggcgggtgat
     4441 ggcccgcaga agggccgcgc gcatcacctc gaagtcggca tcctccttgg cgatccgggc
     4501 caggttgttt tccgccaggt ccttggcccg ctgggcccgg tccttgtcga tcatgtcgga
     4561 gcgctcggcg ctttcgacca gcaccgtgac cttcccggag gtgacttcca cataccccgt
     4621 attcagggcg tagaaggatt ttttgccctc ccgcgtcatg ttcatctcgc cgatttcgat
     4681 cgagctgagg agcgaggcat ggccccgaag caccccgaac tcgccttccg tgccggggag
     4741 ggtgacctcc tccaccttct cactgaagac catcttctcg ggggtcacaa tttcgagaat
     4801 caaatcgtcc gccatctgcg ttcctccggc tactcgctca acctcttggc cttttcgatc
     4861 acttcctcga tcccgccgac catgtagaag gcctgctcgg gcaggtcgtc atgcttgcct
     4921 tccaggatct ccttgaagcc gcgcaccgta tcggcgacgg agacgaactt gccgtccgtg
     4981 cccgtgaact gcgccgccac gaagaacggc tgcgagagga accgctggat cttccgggcc
     5041 cggctgaccg tcagcttgtc ctcttcggag agctcgtcca tgcccaggat ggcgatgatg
     5101 tcctggaggt ccttgtactt ctgcagggtc acctgcacct ggcgcgccac ctggtagtgc
     5161 tcgagcccga ggacgttggg gtcgaggatg cgcgaggtgg agtccagggg gtccacggcg
     5221 ggatagatgc ccagctcggc gatcggccgc gagagcacga ccgttccgtc caggtgggca
     5281 aaggtggtgg ccggcgccgg gtccgtcagg tcgtcggcgg gaacgtacac gcactggacg
     5341 gccgtgatgg agcccttggt cgtcgaggtg atgcgttcct ggagttcgcc caggtccgtc
     5401 gccagggtcg gctggtaacc gacggcggag ggcatgcggc ccagcagagc ggacacttcg
     5461 gaaccggcct gcgtgaaccg gaagatgttg tcgacgaaca ggagcacgtc ctgcccttcc
     5521 tcatcgcgga agtattccgc ggcggccagg gccgtcagcg acacgcgggc gcgggcgccg
     5581 ggcggctccg tcatctggcc gtagatcagg gcggcctgct tgatgacgcc ggactgcttc
     5641 atttcgaggt acaggtcgtt gccttcacgg gtccgctcgc ccacgccggc gaaaacggag
     5701 atgccgccgt ggtgcatggc gatgttatgg atcatctcca tcatgacgac ggtcttgccg
     5761 acgccggcgc cgccgaacat ccccatcttg ccgccccggg ggaacggcac gaggaggtcg
     5821 atgaccttga cgcccgtttc caggacgtgc acggaggtgt cctgctccag gaaggagggc
     5881 gcctcgcgat ggatcggcgc cgtgtgcttc acgtcgatcg ggcccaggcc gtccacgggc
     5941 cggccgacca cgttgaggat ccggccgagg cactcgggac cgaccggcac ggtgatgggc
     6001 atccccatgt ccttcgccgg catgccccgg acgagaccgt ccgtgatgtc catggcgatg
     6061 cagcggacca cgttgtcgcc cagatgctgc gccacctcga cgatcaggtt gtccggctcg
     6121 tcgttgatcg cggcgttcgt gatcgtgatg ccgttcatga tgctgggcag ctttccctcc
     6181 tcgaacgcca catccactac cggtccgatg acctgcacga ttttacctat attcataatc
     6241 atctccttcg ctgatctgtc ttcgcgtatt ttcgcctcgt gtccgtcccc gctttagcct
     6301 ttggcgaggg cctccgtgcc gccgacaata tccatcagtt ccgccgtaat cgccgactgt
     6361 ctcgccttgt tgtactgcag cgtcaagctc tggatcagct cgtcgcagtt ccgggtcgcg
     6421 ttgtccatgg ccgccatccg cgcgccgttc tcgccggccg acgtctccag gagcgcccgg
     6481 aacagaagca cgtgcacgta catcggcagc agcttgtcga ggaggacctc gtcggagggc
     6541 tcatagatgt actccacccg cttttccggg tcgatgtcct cttcctgtcc gatgctgggc
     6601 agggggaaga ggcgcaagac gaccggcttc tgggtggaga cgctggcgaa ctcgttgtag
     6661 atcaggtata gctcgtcgta ctcctcgccg atgaagggcg ggatcacgtc gttgctgatc
     6721 ttgaccgcca ggctcatgtc gaacttgccg aacacgtccg tccgctcgtt catggtggcg
     6781 gccttcttcc gaaaatagtc gcgccccttg cgcccgacgg gaaccagggt cacgtccttc
     6841 ccttgggcca gcttgtcctt caggaagcgc tccgtcgcct tgatcaggtt ggtgttgaat
     6901 ccgccgcaga gcccgcggtc cgaggtcatg cagatgacac ggatcttctt gggatcccgg
     6961 acggacagca ggggatggtt tttcgcgtcc acccggagcg ccaggctgtt cagcacgtcc
     7021 atgaacttcc cggcgtaggg acggaagctc tccatccggt tctgcgccga cttgaatttc
     7081 gacgccgcca ccatgttcat ggccttggtg atctgcttcg tcttgtggac ggcgcctatt
     7141 tttcttttaa tgtccttcag tgcggccatt aaaacgtctc cctcttacgg tctcggttca
     7201 atcacgctcg cgacgaacgc ttttttcatg cattattccg caacgaacac ggtatcgaac
     7261 tcggtcagga cgtccttcat cttcttttcg agatccgggc tgatgacctg cttctcgtcg
     7321 atctccttga ggacctccgg gtacttgctc ttgacgaacg acaggagctg cggctcgtac
     7381 tcgccgagct tgtcgacgcc gtacttgtcc aggaacccgc gcgtgccgga gaacaggacc
     7441 gtgacctcct cgccgaggga catgggctgg aactggggct gcttgagcag ctcgacgagc
     7501 cggacgcctc ggtccagctg ggcctgggtc gccttgtcca ggtcgctccc gaactgggcg
     7561 aaggccgcca gttctcggaa ctgggccagg tcgagcttca gcgttccggc gacctgcttc
     7621 atcgccttca cctgcgcggc gccgccgacc cgggacacgg aaagaccgac gttgatcgcg
     7681 ggacggacgc cggagtagaa caggcccggc tccaggtaga cctggccgtc ggtgatggag
     7741 atcacgttgg tcgggatgta ggcggacacg tcgccggcct gggtttcgat gatgggcagg
     7801 gccgtcaggg atccgccgcc cagttcctcg ctcacgcggg ccgcccgctc gagcagccgg
     7861 gagtggttgt agaagatgtc gccggggaac gcctcgcgtc cggggggacg acggagcagc
     7921 agggagatct gccggtaggc gacggcctgc ttggagaggt cgtcgtagat gatcagggcg
     7981 tcctgcttgc ggtcgcggaa gtactcgccg atgctgcagc cggcgaacgc ggagatgtac
     8041 tgcagggtcg cggggtcgct ggcgcaggcg gacacgacgc aggtgtaagg catggcgccg
     8101 tgcttgcgaa ggttttccac cacctgggcc acggtcgatt tcttctggcc gatggcgacg
     8161 tagatgcatt tgacgcccgt gtccttctgg cggatgatgg cgtcgatgca gatggccgtc
     8221 ttgccgatct ggcggtcgcc gatgacgagc tcccgctggc cgcggccgat gggcgtcatg
     8281 gcgtcgacgg ccttgagccc cgtgtacaag ggctgattga cgggctggcg ctggatcacg
     8341 ccgggggcga tcatttcgat ccggcggaat tccgtcgtct cgatcggacc cttgccgtcg
     8401 atcggcgcgc cggtggcgtc gatgacccgg ccaagcaggg cctcgccgac gggcacctgg
     8461 gcgatcctgc cggtccgctt gacgatgtcg ccctccttga tgtgggtgac ctcgccgaga
     8521 acggcgacgc cgacgttgtc cgcctcgagg ttcaggacca ttcccagaat cccgccgggg
     8581 aactccaaaa gctccatggc catggcgttc tccacaccgt agacgcgggc gatgccgtcg
     8641 ccgacggaca ggacggtacc cgtttcgctg atgtccagct tcttttcata ttccttgatc
     8701 tgtttggaaa tgatctgact gatttcctct gccctgatgg tttccatgcg ctatatctcc
     8761 tcccctaaga gattccttat cttgttgagc tgcgtccgga tgctgccgtc gtaaatcgtg
     8821 tccccgaccc ggacgacaat cccccccacc agggaaggat cgtccatgac ggccatctcc
     8881 acgtgcttcc ccgtcatcga ctcgagcccg ctccgcaggc tctgaaccat ctggggggac
     8941 agcgggaagg ccgtcttgac ctggacccga acgtttttca ggaccgcgtc catgagttcc
     9001 cggtaacaat cctcgatctc ggaaaggacg ctgattctcc gcttgtcgac cagcagcttc
     9061 aggaaattgg ccgtgacggg agagatatcc gtcttctcga gcagcgacgt gaccacggcc
     9121 ttcttatcgg cctggtcgaa gatggggttg tccaggaatt cccggagatt cttgttcatc
     9181 tccaggaggg atgagaactg gcggagctcc ttgtagtagg gctcgtactt ccgttcctca
     9241 cccgcgatct cgaagaacgc cttcgcgtac cgtttggcga tattgctgct gatcaatttt
     9301 gtttcaccac cttgtctatg tagtcgcgca ccatggtctc gtgatcctga ggcgtgatct
     9361 gcttcttcag aatctcctca gccagttcta cggaaagctt gaccgcctcc agcctcagct
     9421 ggctgctcgc cttgttgaac tcctgctcca tgcgggcctg ggcgtcttcc ttcatcttct
     9481 ccgcctgctt gcgggcgtcc tcgacgatcc tcgccttttc ggcctcaccc tgcgagcgga
     9541 tcatctccgt gatgccgtcg atctcgccgg tcgccttctg gagccgtgcg tcgtagtcgc
     9601 ggaacttcgc ctccgcctcc tccttcgccc gccgggcgtc atcgagcgcc gtgccgatct
     9661 cgcccttccg cccgacgaag aactccttca ccttcttggc cagcatccac cacaaaaacc
     9721 acgccagcac cgcgaaattc agcatgcgcc agccgaaatc cttccattcc gcccatttgt
     9781 cgtggccgcc ttcggccccg ccgcccgagg catagaccac ggccgcggtc agcagaaccc
     9841 acccgattgc cgtcaacagg gaacgcgaca caagccgacc gtgccggcgc gctgtaagcc
     9901 accctctcat tggacactcc ttcccaacac tttttcggca atctcgaccg agatccggcg
     9961 cgactggtcg tgcaggatcc gtctcgcctg ggagacctcc tggttcatcc tggcctggaa
    10021 cgtctccacc agccccggga tctcgccccg cacggcgtcg atgatgccct tggcctttac
    10081 ggcgccctcc tgggtgatct cctgcttctt ctccagggcc ccggccttcg cctggcggag
    10141 cttctcctcg taggcggcca tcttctgctc gaccgtctcg ttcagccggc cgatttccag
    10201 ctccgagtcg tcaagctgct ttttccgccg gttgatgatc gcaaggatcg gcttgtagag
    10261 gagaagattg aggatgaaaa tcagggccag aaagttgatt atctggatca ggaatgtata
    10321 gtcgagaccg atcgtcactg ttcatacctc gctttgcggg atcaaattcc aaaaaaaacc
    10381 gggaggaaaa agtaatccac ccggcttctc atcgaatacg ttgtacggaa agctcgctca
    10441 ccctaacttg aaagaggttc tctggatgcc tcttgccatc aacccgattc acaaacaacc
    10501 tcgacgaacc gatttcatga cctgcgcggc agagcggcgc ctgacttccg gtcatatagt
    10561 gactgccgtt caagtcggcg cactactaat cataactcac cctgcttgtc aagcactttt
    10621 tgcccaaata aagcgccgct tttgcacggg atcatcccgg cgaaaacacg gtggattggc
    10681 ccgtccggcg cacccttcat cgaaaagaaa aaaacgcgga aattcccgcg ggccggacgc
    10741 agggggaaat cggcccccct gctcacgaat gcagaatgaa acgcctcgtg ctgatgttca
    10801 gcatcaggcc gacaccggtc aggagcacca ccatggacga gccgccgtag ctgaaaaacg
    10861 ggagcggaat gcccacgacc ggaaggatgc ccgtgaccat cccgatgttg atgaagacct
    10921 cggaaaagat catgaccgtg acgccgaacg cgatcagcgt cccggagagg tctctggcgc
    10981 gctgcgcaat cttgaacccc cagaggatca aaagcaggaa gaggattaaa agcaccagcc
    11041 cccccgccag gccccactcc tccgcgaaga cggaaaagac gaaatcggtc tgctgctccg
    11101 ggaggaactt gagctgcgtc tgcgtcccct tcaggaaccc cttgccgaag aggccgcccg
    11161 agccgatcgc gatcatcgac tggatgatgt gataccccgc ccccagcggg tcccgctccg
    11221 ggttgaagaa ggtcaggagg cgcgccttct ggtagtcgtg cagaaagaac caggccaggg
    11281 ggacgagggc aaggccgccc gcgccgaccc agaggacgga gcgccaccgg accccggaaa
    11341 agacgaccat ggcgatgaag atgatcagca ggatcaggcc cgtccccagg tccggctgcc
    11401 ggaggatcaa gaggaacggg acgagcacca ggatcagggg catgaccagc tcccgcgtgg
    11461 tgtatccctg gaggctccgg tgccggtcga aataccgcgc cagggccagg atgagcgcca
    11521 gcttcatcat ctccgagggc tgtagcatga agcccccgag ggcgatccag cgctgcgacc
    11581 cccgggttgt gtagccgtag agcgccacga cgaccagcag cacgacgccc accccgtaaa
    11641 tgacgtaggc gtagcggtgg atcaggcggt aatcgacgct gaaggcgatc accatggccg
    11701 cgaggccgat catgatccac tggagctgct tgaggtacag cggtgtctgc cgaaggcccg
    11761 agaggctgta gccggtgctg aagatgttca tcaggccgat gccgctgatg acgagtacca
    11821 gcacgaggag cgtccagtcg aagttgagga gcagtctgcg gtcgatcttc atgggttcgg
    11881 ctccgggggg gcgttcgctc cgcggggcgc cgcggccgga gcggcgttcg cgacgaccga
    11941 gcgcggcggc gcgggcgggg cgtttttgcg tgcaaagtag gcgtcgatga ttcgccgggc
    12001 gaccggggcg gccgttgccc cgccgtggcc gccgtgctcg gcgatgacgg cgatcgcgat
    12061 ttccggggcc ttgtccgggg caaagcagac gaacagggcg tggtcgcccc gggcgacggc
    12121 cagcttgagc cgccgcgcct tttcatcctg gggcagcccc accgcctggg cggtgcccgt
    12181 cttcccggac acgtccgcct cccgtcgccg caggacgtag ccggtgcccc ggggctcgtt
    12241 cacgaccccc cacaggcccc ggttgatccg cttgagcgtc tcggccgaga tggacgccac
    12301 ggaccggcgc acggggggga actccctgac cggcttgccg tcctgcgtct cgatccgctt
    12361 gacgaggcgg ggctgccaga cggtcccgcc gttggcgagg gcggcgtacg cattcaaaag
    12421 ctggatcggg gtgaccaggt cgtagccctg gccgatggcg atggagatcg tctctcccgg
    12481 gatccagggc tgcctgaagc gcttcagctt ccattcccgg gtcgggatga gccccgcctt
    12541 ctcccggggc aggtcgacgc cggtcggcat ccccaggccc agctcgcgcg cgtagcgggc
    12601 cagcgtgtcc actccgacgc gccggccgac ctcgtagaag aaaacgtcgc acgattcgac
    12661 cagcgcccgg tgcagattca cgcggccgtg cccctttctc tgccagcagc ggaaggtccg
    12721 gttgcccatg acaaagctgc cgttgcagta cacggtggtc tcgggcgtga tcagcttttc
    12781 ctccagggcg gccgcggcca cgacgggctt gtaagtcgaa cccgggggat actgcccgga
    12841 ggcggcgcgg ttctccatcg ggcgcagccg gttgtcggcg agcttccgcc agtcggcgag
    12901 cgacaccttc cccgtgaaca ggttgggatc gaaggagggc gtgctgagca tggccaggat
    12961 ggcgccgtca cgcgggtcca tgaccaccac cgcccccgtc cggcccgcca tggcgtccca
    13021 ggcggtcttc tgcaggtagg aatcgatgtt caaaaccacg ttgtacccgg agacggggtc
    13081 gatccgcccc agcgagcgca cacccttgcc gaccgcgttg acctccacct gctcgaagcc
    13141 gttcgtcccc cgcaggtacg ggtccaggta cttctcgatg ccgaacttgc ccaccatgtc
    13201 ccccatggcg taggcgcccg cggggttgcg ctccagctcc tcggcgtcga tctcgccgat
    13261 gtagccgatc acgtgggcca tgacctcccc ggccaggtac tggcggaccg gcgacacctc
    13321 ggtgatcagc cccggcagtt ccagggcgtg cgtctccgcc agggcgagct tctcccaggc
    13381 gacgttccgc tccaggcgca agggctgaaa ggtccgctgg cgtcccttcg cgggcacgtc
    13441 cggcgcgaac gccagggagc gcttccggca cagggcggcg aacttatcga cggtctggcg
    13501 gatgtcgcgg gagcggtccg ggatgaacac gagatcgaac gaggtcttgt tgtccacaag
    13561 caccgtcccg tttgtgtcga ggatcagacc gcgcagggcg ggaagcttcc gcatccgcac
    13621 gctgttgttc tccgaccgct gccgcagctc ctcgcccttg atcacctgca ggtaccacat
    13681 ccggacgaac aggatggaga gggcgatgaa caccaccacg aacagcgcct gaaagcgccg
    13741 gcgaaactcg ccgggctcgt ggctgttcag ggggttggag ttcatcgaca tgggtcaggg
    13801 ctcatcgaca cgggtcaggg ttgatcggca cgggccgggg ttcacggcat cggtcggggt
    13861 tcatcgacct cgaggaacga ccggacgcgg tcgaaaacgg gaaagaccgc cgggctcaag
    13921 accccgagga ccagcgcctg gagcacaaag acgcgcagcc ggccttcgag cggcaccccg
    13981 tggatcagcg ccgtcagccc caccacgagc aggccctcca acagcgtgca gacgatgacg
    14041 taggtcatca ccagggtccc ctgcttgacg tacacccgcg cggaaagcgt aaacgagagc
    14101 agaaagacga gcgcgtaggc cgtcatgtac agccccgtga tcgacccggt caggcaatcc
    14161 acgaagaacc ccatcagaac gctcagcacc gccccgcgcg ccaggtccat ccggaagccg
    14221 gcgtagatcg cgagcagcag cgacacctcc acgcttagtt gctgaaagaa aaagaggttc
    14281 agaaacccct gctggaagac gacgagacag aaggcgagaa acggggcggc cagataaaac
    14341 agcacgatcg ctacctcccg gtcagcacga gaacttcctc cagccgcgag aagtcggccg
    14401 ccggcgtgac ctcgatcttc tggaacatgg cggttgtcct ctcaacctcg cgcacgaccc
    14461 cgaggagcag gcccttggga aagatgtcgg ccatcccgga ggtcaccacg gcgtcgccgg
    14521 cgcggaccgg ggctatcttg ccgacgtact tcaactgaca cccccgccct cccctgccct
    14581 gcaggatgcc ctgcacccgg ctgggctgga gcaccgcgtc cacgttgctg ctctcgtcga
    14641 cgatcagcag cacgcgcgcg gcgtgccagg aggcctccat cacgcggccg accacgccct
    14701 cggcgacgat caccgcctgc cccggcttga tcccgtgcgc cgacccccgg tcgatcacga
    14761 gcgtccgggc ggtcgcgctc cggacgtacc ccgtgacccg cgccgtcgtg ccccggtagc
    14821 cgtcgtgctc cttcaagtcc agcagcttcc gcaggcgccg cgactccagg tagccctcct
    14881 ggtactggac gagccggcgg gtcaggatgg cgttctgccg gcgcaggcgg ccgacctccg
    14941 cctccaggcc gaacaggaac aggtaccgcc gccaggcctt cgccgccgca tggatcggcc
    15001 ggcccagcag gccctcgacg ggggcggcgc actccaggac gatttgacga aagacgccgg
    15061 tccgcccggg atgccggatg ctgatcgata tcacgacaag acagaggaca gccaggaggg
    15121 tcacaacgat gacggttcgg tatcgctgaa aaaacatcat aggcgtgtcg ggtgccccgg
    15181 accggcggag ggccggatcc ggacggcggc cccgcggcgg gcggaaacgg gcggagaatg
    15241 tccggacgca gacgtcctgt cgcggctaca actggaccgt gatctccttc aggacgtcga
    15301 gctcgtccag ggccatgccc gcccccttgg cgacgcagga cagggggtcg tcggccacga
    15361 tgatgggcag cccggtttcc tccctgagca ggacgtcgag attgcgcagc agggcgcccc
    15421 cgcccgtcag gacgatgccg cgatcgacga tgtcgcccgc cagctccgga ggcgcgtttt
    15481 ccagggcgtc cttgatggcc tccacgatca tgctgaccgg ctccgcgatc gcctcgcgga
    15541 cctcctcgga gtcgatctcg atggtcttcg ggatgcccga gatcaggtcg cggcccttca
    15601 cgtccacctt gcgcagctcc tggtccgggt aggcgcagcc gatggtcgtc ttgatcatct
    15661 cgcccgtgcg ctccccgaca agcaggctgt acttgcgctt caggtactgg acgatctcct
    15721 cgtcgatccg gtcgccggcg atgcggacgg attttgcata cacgaccccc gccagggaga
    15781 tgacggccac ctcggtcgtg ccgccgccga tgtccaccac catggagctg atcggctcct
    15841 tgatgggcag gccggcgccg atcgccgccg ccatgggttc ctcgatcagg tagatctcgc
    15901 gggcgcccgc cgattcgacg gtctcgcgga cggcgcgccg ctccacctgc gtgatgccgg
    15961 aggggatgga gacgatgatc cgcggccgca ccagggttcg ccggttgtgg acgctcagga
    16021 tgaagtgcct gagcatggcc tcggtcacgt cgaagtccgc gatcacgccc tcgcgcatcg
    16081 gccggatggc gacgatgttt cccggggtgc gtcccagcat cttcttggcc tcggaaccga
    16141 cggccaggac cttcttctgt ccgcgggcgt ccttgtggac cgcgaccacc gagggctcgc
    16201 tcaggacgat ccccttgccc ttgacgtaga ccagcgtgtt ggccgtcccc aggtcgatgg
    16261 ccaggtcatt ggaaaatttc ccaagaatga aatcgaaaag caacgtcccc tcctcaattg
    16321 tcccgcaaga attccgctgc gatcccgctt ttgcccccga taccggagcg cgatgtgtat
    16381 atcccatttc cgggcccttg gcaatgcatt tttccagaaa tgatttgcat tttggggatg
    16441 actgtactac taatgccgcc catcaaacga ggagtggaac gccatgctcg agctgatgag
    16501 aaagcatgcc cgcaactgga tcatgaaggt cctgctgggc atcatcattc ttgtcttcat
    16561 tttctatttc ggatccatgg gggggcagca gcaggccgag acggtcgcca agatcgacgg
    16621 gaagatcctc gcctacgtcg acctgcagaa ggaataccag aacctgaccg aactctaccg
    16681 ccagcgcttc ggcggcaacc tgcccgagga gctcctggac tccctggacc tcaagcagaa
    16741 ggccttagac accctgatcg accaggccgt catcatggcc aaggccaagg acatgggcat
    16801 cgaggtcacc gacgaggagg tgcgggcctc gatcatggcc tatcccgcct tccagcgcga
    16861 cggcgtcttc aacgacagcc tctaccggcg gatgctccag tacaacaagg tgacgccgga
    16921 ggaattcgag gcgatgcaga agaaggggct gacgacggtc aagctggaaa ccctgatcca
    16981 ggaggggatc aaggtgtccg accgcgaggt ctacgacctg taccgcgcgc agaccgaaaa
    17041 gatcagcgtg agctacgtgc agctcgcgcc ggcggatttc gccgggcgcg tgcggccgac
    17101 ccgcgccgac ctggaggact acctccgggt gcacggggac cagttccgcg tcccggagca
    17161 actccaggtg aaggtcctcg ccttccgggg ggccgattac gcaacccagg tgaaggtcac
    17221 cgacgacgag gtggccgaag cctacgagag ccggaaagaa acgttcaccc ggggcggcaa
    17281 gacggcgccg ctcgcggcgg tgcgcgaggc catcgcggcc gagcttcgac acgtgggcgg
    17341 catggaccgg gcgtccgacg aagcgaagca ggcgcacgac acgatctacc aggaggagaa
    17401 tttcgagggc tacgccgggc agaaccgcct gaagctcgcg accaccgact tcttcccgct
    17461 caaccggccg ccggcggagt tggccggcat caaggacttc gccaagaccg cattcgccct
    17521 gcggaaggga gacgtcagcg gcgtcctgtc ggacgagcgg ggctactacg tggtgaaggt
    17581 cgaggcccgg aaaccggcct acgtgccgga gctcaaggcg atcgaggcga acgtggcgcg
    17641 cgcctacacg gcggaggagg caaagaagct ctgccggcgc gaggcggagg agatgctcgg
    17701 gcgcctgaag aagggcgagg gcttcgccgc ccttgcgggc gcgaaggggc ttccggtcgg
    17761 gaagaccggg ttcttcatcc ccgggaccgc cgtccccaaa ctggggcttt cccaggagct
    17821 ggccgaggcc ctcttccaga tctccgagaa gaaaccctac ccggacagcg tcgtcgaaat
    17881 gggcgagcgg ctcgtcctga tccgcttcaa ggcgcggcag gcggcgagcg acgcccaatt
    17941 tgccggacaa aaggacgccc tgcggggcgc gctcctgacc gccaaacgca acgaggcggt
    18001 gcgctcatgg ctggaggaaa ccaggaaggc catgatcaag agcggccgcc tcgaaatcac
    18061 caaagacctc aaagacctct aaaaaatagg gacagcgccc tatttttcat tcggtcatct
    18121 cacggctcat gtacccgata cacgctgtcc acaacgcgtc tcgtggaagc gggtttttct
    18181 cccaggtcac tcgttaattc agacagcgga aaccgaccgt cccctttctt cttgtagagt
    18241 tcaaccgatt tcttcaggta aaccgccagg ccgcccagac tggtccgata gatgaaaccg
    18301 tgcctgatcc gcctgttttt gtgcaacgcc tgttttgtga ttcccaaaat cgccgccgtt
    18361 tccgaggccg tcaagaagtc ccgaatcggc tgctttttca gaatgtcctc cagcagcgct
    18421 tccgcggcgt cgtcaaaggc tcgtgcagtc tccaagggaa ggatcagttc tccacatcga
    18481 tcgcattctt cataatcgac acgatccctt ttcaccggtc caacgaacgg atcctgaatt
    18541 tccagggaag cgcgtttctt gacgtatttt cctccgcatt cgcacggatg cataatatca
    18601 cctataattt cttgaaactg ttgacaatca gtcgcccctt gaggatgtag aaatgaagat
    18661 acacggactc agtgttcaga tcgcgcgccc ggtatgaatc tatcatgaga ttcttgatca
    18721 tccgggatgg ttcgcttttg aaaaaatggg aaggtttcag cttctggatg gcggcgacca
    18781 tctcattggt tcgccagccg aaatcgactc gtgctgaatc ccttgcgttt gccttaaaac
    18841 taacggcgcc ttctttagcc aattgcttga cttccaccag cgagtaatgc ggttttgacc
    18901 ttttcaagtg gactgttgga cctttcgtcg tcattaacct aacaccgaag gttgacaatc
    18961 gtcaaccaat atctgtaaac aggtgagccg ctctcctggt ttattaggga cagcgcccta
    19021 ttttcttcag gcacgtgggg tcggggcgga gggtgaaccg aaaaatgggc gctgtcccta
    19081 tttaaattgg cctccgatta cgcatttgaa ccaaccgggg catgcgtttt gatggagagg
    19141 ctgaaaccca attcaccgag cattcgattc agtgttctca attccggatt accccgtttc
    19201 gatagtgtgc ggtacaaact cacacgattg agatccacct tgtccgcaaa tcgggaaata
    19261 ccgccgcgag cggctattac atccttcagt gcaagcagaa atacttcctg atttgcatcc
    19321 tccagcgctg cattaagata ggctgccgct tcttcgggat cccttaacgc ggtcaacaat
    19381 tcctctttgt aagcgcttgt ccttttcatg attcctccaa gtatgccttc caataccgtt
    19441 tggcccgctc aatatcccgc ccctgggctc gctttgttcc ggcacacagc aacaagatta
    19501 cggtcaagtc cacacggcta aagtagattc gatacgtata tttccaggga aatctgacgt
    19561 tccatggtgt agcttatata ctacaaacca aaacaaaaag ggaagaaaaa aatgaattaa
    19621 atagggcgct gtccctattt aattttgaga aaatcggcga cggaaaggat cgggatgtcg
    19681 cggaacgcgc ccagatcgag gaggtcccga tcgccgctga caatggcatc cgccccggcg
    19741 gccacggcgc aggagagaaa aatgtcatcc ccagggtccc ggcagacccc ccggatctct
    19801 tcaccggtct cgacgacctc gcaccagggc aaaatttcct cctcgatcag ggcgttgatt
    19861 tcaggccccg tgagcccaaa tcgcggatag gcgaggaccc tcctgaactc ggcgaaggtg
    19921 gcgcgcgaaa aaagaggaat cagatcgccc gatttccaac ggccgatcag ggcgtccagt
    19981 ctgccgccga agagaagcgc cgacaccagg acgttggtgt ccagcaccac gcgcttcatt
    20041 cggacttttg ccgggaccac cggacagcct ccgccacgtc cgcctcggag acccccagcc
    20101 gccgcatttt ctcccggatg gcctgcaggt tgcccgcaac cggctggatc cgcaccggtt
    20161 cgaggacaat ccgcccgtcc tgaaccacgg cgtcaaagta
//
LOCUS       DAOOLJ010000030        19863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2720_length_19863_cov_16.332795, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000030 DAOOLJ010000000
VERSION     DAOOLJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 19863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19863
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2720_length_19863_cov_16.332795"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..1871
                     /gene="cls"
                     /locus_tag="PLW16_06440"
     CDS             432..1871
                     /gene="cls"
                     /locus_tag="PLW16_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPB70578.1"
                     /translation="MNTLDTLMAGLAFVVAMVSSLHALFLKRDPKSAIGWITVCVLIP
                     FAGPFLYYLFGINRVRTRAEKLQRRAGSKQKGATPRADRQTSSPEGSAGIKDPSDRPS
                     FARVSDILTDRPLVDGNRIEALHSGEEAYPAMLEAIDGARRTLFLTTYIFERNRTGKA
                     FVDALLRAAERGVDLRVIIDGLSDVFHLRWASRRLKKSGVPVARFLPPRLIPPTALIN
                     LRNHRKILVADGQIGFTGGINIGDSYLAGRHDNPHRVVDLHFRLKGPVVRQIEEVFID
                     DWGFITGTYESVAAAESAPVGSARCRVITGGPNEDMDKLAMILTAAVAAARTSVVIMT
                     PYFIPPRGLLVALQTAALRGLRVSIVLPEKSDSRVVHWATRNLLWELLERGVRVYYQP
                     PPFVHTKLVIVDDGYVHIGSANIDPRSLRLNFELNVEIFDKPLTGTLTRDVKAVIQKS
                     RAVTLDELDGRPFPVKLRDSLAWLLSPYL"
     gene            1987..2820
                     /locus_tag="PLW16_06445"
     CDS             1987..2820
                     /locus_tag="PLW16_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB70579.1"
                     /translation="MVEKDLVYRGKSKDVFRIPDGPHKGKYRFVFTDRATGYMEGRRP
                     VFDPGYDIVVGKIPGKGAIACRFAAHFFRLMKDNSVPTHYIDTIGENEMIIEPAVPLG
                     MEVKSPEFPGSAPLTNLEFTWRNNATGSFWRRYPFVTPCKNVHRVVEAWTKGDSDILI
                     TMEALEETGALTKSEIEQSVKLVKEIAEIVSGEFASRGLHVIDGKFELGRLRDGDGKI
                     VLIDEISPDVLRVCRGFEPDRDGNCTHFKECAVTHYSDGKRTIKNKNQVSAADFIDIF
                     L"
     gene            2937..3824
                     /locus_tag="PLW16_06450"
     CDS             2937..3824
                     /locus_tag="PLW16_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB70580.1"
                     /translation="MKRDVLKRDLRFAIIPKVVHPWFEEVYRGAIAQAELLENHLGIK
                     IEVEYLTPETADLAVQRAIFDKAIAADYDGITIDPLDKLFNMKSIADIKNKGIPVIVF
                     DSPAPVAGILSVGNNFTQQGTVAAERLAKLLEEKGKVAVMQGYPSAPNHHERYVAQIA
                     VLKKYPGIVIVDGGIDNDDIDVAKQQALSVLEAHTDLNGYLCCDASGPIGIAAAIKKA
                     NRVGIVKVVGMDGIKPILLSIKEGVIESSVATIPRMQGSMSILMLWQASLGVQIPRKI
                     DTNIDIITRDNVDNFLALA"
     gene            3951..4385
                     /locus_tag="PLW16_06455"
     CDS             3951..4385
                     /locus_tag="PLW16_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70581.1"
                     /translation="MNRMEIDIEALIPHRGRMRLIEGVIAVDDERAVTTAIASADWPL
                     YRDGSVDVLVTVELVAQSAALFEGWKRKQSGKGGAKGWLVGIKSAQFQRSRIAVPAIL
                     ITEVRRNYALESYAVFDGTVRAGAELVAALSIQAFRPEEEDA"
     gene            4413..5555
                     /locus_tag="PLW16_06460"
     CDS             4413..5555
                     /locus_tag="PLW16_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70582.1"
                     /translation="MELIPYREEHRIFRETLRRFLAREIVPEIEAWEEAGIVPRRAWK
                     RMGEQGFLCLEVPEAYGGAGADFLYSLIVVEEMTRTNHCGLSARLHSTVVVPYVTAFA
                     AEAQKRRYLPHCVSGDCITAIAMTEPQAGSDLAAIRTTAVEDGDAIVLNGQKTFISNG
                     INGDLFVVAARDPAVEKPHAAIDLYLVEAGTPGFAKGRRLKKAGWHSQDTAELFFTDC
                     RIPRENRLGQKGTGFRILMQNLQPERLVLAVSAVAAAEMVFELTLRYCRERSAFGKPL
                     AKFQHIQFELAEMSTEIRLGRTFVDKLVADHIEGKAVVTEVSMAKYWTTEMAFRVADR
                     CLQLHGGYGYCEEYPIARAWRDLRVTRILGGTNEIMRGIIARRLGL"
     gene            5621..5878
                     /locus_tag="PLW16_06465"
     CDS             5621..5878
                     /locus_tag="PLW16_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPB70583.1"
                     /translation="METLMAELKVKIVDTLSLLDIAPEDIGDEERLVGGDLGIDSIDV
                     LELVLMLEKDYGVKIESREVGVQAFASVRALAAFVREHRKK"
     gene            5953..7437
                     /locus_tag="PLW16_06470"
     CDS             5953..7437
                     /locus_tag="PLW16_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70584.1"
                     /translation="MTTDSKLDTLVLGSGIGGLSMAVLLARLGFRVAVIEKNPLPGGL
                     LRSYRRGGLDCPVGVHYFGAFGEGEPLRRMCDFMGVTEGIAAERMGQGGPIDRYLFDD
                     FTFDLPEGLDAFTGALHRAFPQDAQCLDAIAGNLRVMADLQNSFAFLSAMPPLMNLDL
                     FRSLDAYLSGMGCSLPLKSVLNVSASWVGMSGAECPVLYHHLALTSYLQSSWRLKGRG
                     SDLAEAFVSRLDTLGGRLILGDPAEAILASGGAVTGVRLASGRVLSADRVVAALHPKT
                     VLGMLPEGALKPLRVRRIRRLAETEGLFVVSAAVDASAHPALPHNLFRLRADRDGGLA
                     EGVFYQLLDGTEEWNLLMIITKSLFAEWRRWEDTMTGRRGADYEAEKAGRADRILRQA
                     EEVFGPFHRARVLDTYTPLTLRDWTGSPEGAPYGILRSTRQLPAAATLHRPTLAGLYL
                     AGQNALSPGVLGTVLGSFQVARQMIGQERFSREVLEPLRSTPAR"
     gene            7564..7863
                     /locus_tag="PLW16_06475"
     CDS             7564..7863
                     /locus_tag="PLW16_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPB70585.1"
                     /translation="MTREQIQTAILEIFRREFEIENPGLDENLREVYEFDSVDAIELL
                     LEIERFLGSELTHDEKKGAMEIRTLNQVIDYVDGLAGKRGLTKDAPTRKEASSRP"
     gene            7860..9098
                     /locus_tag="PLW16_06480"
     CDS             7860..9098
                     /locus_tag="PLW16_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPB70586.1"
                     /translation="MKGRRVVITACSAITPIGHGRAAILDSLRRGVSGVKPLREDNLL
                     AKYIHSQVFGTVDDPIAYDFKRTYRKTMGPVAYYACQVAKEVLEQAGLPPEFVTSGRL
                     GVAFGSTHGSPTVQREIYKTFFSGTRAEYSSIGAVDYLKSMVHTTAVNITKMFGITGR
                     VISSSTACTTSSQSIGYGYEAVKFGMQEAMLCGGADEYDTTTVAVFDNLLACSTAFND
                     RPQCTPRPFDRQRDGLVVGEGAGAVLLEEYEHAKRRGAEILGEVIGFACNNNGGDLIL
                     PNLAGIRATLQSSLEVAELNADDVDFVSAHATATRMGDVIEAQAIHAVYGDNPFVTGF
                     KGYMGHTMGSCGAIETILSLYQMQEGFIAPTLNLEEPDERCAMIRHTLKLEERPVTVA
                     AVQNFAFGGVNTSLLIKRFA"
     gene            9104..9883
                     /locus_tag="PLW16_06485"
     CDS             9104..9883
                     /locus_tag="PLW16_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB70587.1"
                     /translation="MVTFPRLFKPLVDWAVTMILWTHFTLGFCVFFAPRYVWACFRAR
                     DREAAFQRLNHRYFRSFFRLVQWITPRLSLRIDAEVRTVRSAVIVCNHLSYLDPILLI
                     SLFEKQKTIVKSVFFRVPIFGWVLRRSGYIPSKTGGDYTSLMIRNIETIRGYLDAGGN
                     LFIFPEGTRSRTGKLGPFNKGAFSIARYCRAPVVCLAIRHSDRLFIPGRFRFNTCVSN
                     VLSVARIGSFDPDYGDPAFRLGDLIREIRTLYEARQAQDSD"
     gene            9914..11173
                     /locus_tag="PLW16_06490"
     CDS             9914..11173
                     /locus_tag="PLW16_06490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPB70588.1"
                     /translation="MENITENERVVITGMGTVNAIAGDPLEFAAALKDGSCGIGPVTR
                     FDTTAFRTHNGAEVKDFHPRALLPPEFSLKRVSRADAFALVAAFQALSQAGLYPVPES
                     LRERTGTVIGGGAGGLPEAEAFYQDYLRRGGGRGRFSRLATVACAAHANAIATQFGLY
                     GPKATIMTACSSGATAIGLGRDLIRTDAAEAMIVGGAEPLCRVTFASFNALQAVDPGY
                     CRPFSGNRAGLTLGEGAGILILESLAGARARGAAILGEVLGYGVTCDSHHMTAPDTGA
                     SGAVRAIRAALADAGIPPDAVDYINAHGTATPPNDLMETRAIKAVFGPRAGRIPVSST
                     KSMHGHTLGASGALEAVVSVLAITERFIPPTIHCETPDPECDLDYVTTGARSGTPGIV
                     LSNSFAFGGNNTAVVFGCWRDEGRGRG"
     gene            11166..12458
                     /locus_tag="PLW16_06495"
     CDS             11166..12458
                     /locus_tag="PLW16_06495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPB70589.1"
                     /translation="MDRQVVLTGIGMVTPLGVGKAEFRDALWRGETGIREISAFSTAG
                     LGSHLGAEVRDFVPRDFVALKTLRKMDRLSAMTTASVRMALDDAGLAVTDANRDRIGI
                     VMGTAFGNTDLKVQSARVLFTEGPGRVSPMHVPNTVMNAPAGHASIELGFRGVNTTIN
                     HQAVSGEAAIAYGAMEVRRGVADVILAGGADLLSPFFYEALCRFRALSPTDNGVEAAR
                     PYDLRRNGPVMGEGCGIVCLEAREHALARGADPYCELAGWGLAASPASPTGWPDSPRG
                     LLVALRRALDMAGVVPAGAGSAGSDGGPQDDRRRPGGGVPTGIDCIQGAGNGGRDPDA
                     LEAMAYRDLFGGSEGMPLITSLKGATGEIFASGGIRAAALALSIREGVVPPVVGLAAP
                     LMPLPFVTGEARRRSVRRALLSGLSFGGTYAGLVFSAV"
     gene            12740..13171
                     /locus_tag="PLW16_06500"
     CDS             12740..13171
                     /locus_tag="PLW16_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HPB70590.1"
                     /translation="MADLLELATDIVIAHASTTEMTSDDLMSEIKAVYATLKGLEQGE
                     AGDVPAKPALTRRQAFKKDEVGCMICGKTGLTTLKRHLATAHEMKPGQYRKRFNIPKD
                     QPLAATNYVEKKRQIALDRGLGKNLAKARAAKTTKVKKAKK"
     gene            13168..14736
                     /locus_tag="PLW16_06505"
     CDS             13168..14736
                     /locus_tag="PLW16_06505"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70591.1"
                     /translation="MSWGQVNVGEWITKRALTHPDRPFLCEEGMRNYTNREFNERVNR
                     IAHALAAWGAGRGERVAVLMANASEFLEIFFACAKTGAIMTPLNVRLAVPELVYILKD
                     CAPRVLVYGADFADKVAEIKALLTQLPFCVRHGGDTGQDPDLARAVADFPDAEPAPSP
                     ELALDDPLFTMYTSGTTGDPKGAVLTHGNVLFAAINTVMGYGINRSYKSLVVAPLFHI
                     GALGASALPILYAGGSLYIKGFYNAAEVLDLIVREKINYLFAVPVMFQLLAQTEQWAQ
                     ADFSHVHFFIAGGAPMPLPMIQKYQTEKGVSFAQGYGMTEAFRLTSLDLEDAVRKAGS
                     VGKEVLHVRLRIVDEQDRDVPPGEAGEIVVKGPNVFAGYWNKPAETESAMRDGWFHTG
                     DMGRRDEEGFVYIVGRKVEMIISSGENIYPAEVERVIQALPEVREAAVVGMPDRKKGE
                     VVAAFVLLKEGAALNAEALLEAIRDKIAYFKLPRKIIFVGDFPRNTAGKILKKDLKEK
                     VSGSEGRYRVREGL"
     gene            14733..15461
                     /gene="fabG"
                     /locus_tag="PLW16_06510"
     CDS             14733..15461
                     /gene="fabG"
                     /locus_tag="PLW16_06510"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPB70592.1"
                     /translation="MSDRQTAIVTGGSRGIGRAIAVELASGGYDVVITYRANEAAAEE
                     TLKMIAAAGGRGEALRFDVGDPDAADAALGDLLVRMERVDVLVNNAGITADGLFLMMP
                     RHDWDAVIKTTLGGFYNMTKPVLRKMLRKKRGAIVSMASVSALVGNRGQANYAAAKAG
                     LIAASRALASEVARLGVRVNVVAPGLIATDMIKDAPVENIKEMIPMGRIGRPEEVAKV
                     VRFLCSDDASYITGQVISVNGGMF"
     gene            15470..16102
                     /locus_tag="PLW16_06515"
     CDS             15470..16102
                     /locus_tag="PLW16_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPB70593.1"
                     /translation="MPRGIARRGRFGLLGLLMLLPLLTAWADSWEGLREAAQGITSIE
                     ARFVQKKTLPMLTRPLLSEGRFSYQAPDRLRWEYDRPVRSVLIVNGRDVKRYRKERDG
                     WREETGAALSVMQTVMEEILHWQQGRFDANPHFQATLETGSKPKVTLTPKDPAWRKML
                     RRIELTLSRKQSGVMQSVRVVEDERSFTQIEFGQVAVNRPLPAARFRDIE"
     gene            16099..16779
                     /locus_tag="PLW16_06520"
     CDS             16099..16779
                     /locus_tag="PLW16_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70594.1"
                     /translation="MKAFGATFLLGLALLAVGGCAALPPLTPLDPSQKPTVLSGCREP
                     FLKEKYRLVHVLTATLPGGSESAAIGVLVADPGPRRFQTVIMTLEGWVLFDATVGEAL
                     VVNRAVPPFDGRAFVEALVEDIRLAMFPPEGEPIAWGRGEGGAAVCRFARPDGTHMDV
                     LRTGEDAAEVRLYGTGQGLRKRVRIGSWSGSGPAGTLEIESRTWPSYALYLRLLEQER
                     LETEKTLP"
     gene            16776..18068
                     /locus_tag="PLW16_06525"
     CDS             16776..18068
                     /locus_tag="PLW16_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB70595.1"
                     /translation="MKFRLIYPRWKKLDRQTEFHLPPHGPVVFAATLPDDWEVEFIDE
                     NVQPLPENSDADVVGLSMMLTSQMKRGWEIADDYRRRGVKVICGGIGTMLHAEETMRH
                     ADAVFLGEAEGRMAGVLDDWRHDRLKPVYDYFHDHPPTEAIGTARREILNRDFYNYKG
                     IQMVDLVHASRGCRFNCYPCCVSYLGGRKFRPRPVDKVVAEMAAIDNNRLFIVDNSLA
                     QDRNWEIELFREMIPLKKKWCCHPIEEDDTVLDLAAQAGAWYVYQAVFDMSDHIRRRI
                     RRYHDHGIAVEGTILLGLDDHTEDYIKRFIDFLMEIELDLAEFTVLTPFPHTRTFDDF
                     EREGRILTHDWDAYTADRVVFQPRHMSPERLQELYAEAWDTFYRDEPQNYKMFKLFKT
                     VVEREKADGTFRPRRREMATRRFGRRGGGGGDTPAAAN"
     gene            18139..19689
                     /locus_tag="PLW16_06530"
     CDS             18139..19689
                     /locus_tag="PLW16_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPB70596.1"
                     /translation="MSKKKDGIKNVLIDGTTVSLDGIIAVARREASVRLSERPAFRER
                     LERSRRGLLAAIAADVPVYGVNTGYGKSCGNRLKKNAVLGNGGNMMRFHGCGTGETLG
                     VEETRAAMLCRLLCLARGYSGVSYALLEQLAAFLNLGLTPVVPSEGSVGASGDLTPLS
                     YILAALAGERDVVYAGKRMPARRALKRAGLKPHLFGAKEPLAMINGTSVMTGIAAVVL
                     DRADYLLRAAVDATALSVHALQGKAHHYHPVIGEAKPFPGQIHVADRLRKHLQTRAPE
                     AQLECEGPETLQDPYSLRCAPQVVGVLDDALAWIRPWVEIEANSANDNPLFAEGVEHP
                     LMGGNFYGGHLAFAMDGLKAALASVADMADRQVVLLVNPQVNRGLPGDLVRVDGPERF
                     RHHGFKAMSITASALTAEALKQTMPAASFSRSSESHNQDKVSMGTIAARDADRVCTLT
                     ERVVAIHLLAAAQACEIRGRLGDRPALAKKVAAIRKLAQPTWEDRPMDADIESVARAI
                     SRGEMADG"
     gene            19682..>19863
                     /locus_tag="PLW16_06535"
     CDS             19682..>19863
                     /locus_tag="PLW16_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70597.1"
                     /translation="MDNTERPSYEVRLKVPFFDLDPMQIVWHGHYLKYFDIARSELFD
                     HLGVDLFEYHDRTGYI"
BASE COUNT         3707 a         6159 c         6422 g         3575 t
ORIGIN      
        1 tcctatcatg ttactgtgaa gataatttct ggtggtggtt tttgatagag agtatatttt
       61 ttgagaacgt tattaaggca attctaatcc cttattacaa taacagaact aatcagaaat
      121 agcggaaacc gtatttctct tggcgacgct acggccaagg agaacctcgc ccgctttgtc
      181 tgaaatccag aataaatgcc tgtgtggcag atagaaagac ccagagggaa cagattttct
      241 taaatgcatg ctgccgcatt gcgccttcaa catgtccatc aacgacaaac cccacatctt
      301 gtggaaaaat attcttgacg cagaagtgag cgctccttat gctgccaccc gtaacaagga
      361 cgttcttgtt cactattcgc ttctgatgtt ctgatagcag cggtatacgg ggaaggccgg
      421 tacttgattc catgaatacc cttgacacct tgatggctgg tctggcgttc gtcgtcgcca
      481 tggtcagctc actccacgcc ctttttttaa aacgtgaccc gaagtcggcg atcgggtgga
      541 tcaccgtgtg cgttctcatc ccctttgccg gccctttcct gtattacctt ttcggcatca
      601 accgggttcg gacacgggcc gagaaacttc agcgccgcgc gggctcgaaa cagaagggag
      661 cgaccccccg ggcggacagg caaacctcaa gcccggaggg cagcgcgggc ataaaagacc
      721 catcggaccg tcccagtttc gcccgtgttt cagacattct cacggacagg ccgcttgtgg
      781 acggcaaccg catcgaggcg ctgcacagcg gagaagaggc ctatccggca atgcttgagg
      841 caattgacgg ggcgcggcgg accctctttc tcaccactta tatttttgaa cgaaaccgga
      901 ccggtaaggc ctttgtcgac gcgctgctcc gtgcggccga acgcggcgtt gacctgaggg
      961 tcatcatcga cggcctcagc gatgtctttc acctccgctg ggccagccgg cgcctgaaga
     1021 aaagcggcgt gcccgtcgcc cggtttctcc cgccccggct gatccctccc acggcgctca
     1081 tcaacctgcg caatcaccgg aagatccttg tggccgacgg gcagatcggg ttcacaggcg
     1141 gcatcaacat cggcgacagc tacctggccg ggcggcatga caatccccac cgtgtcgttg
     1201 acctgcactt ccgtctgaaa gggccggtgg tcaggcagat cgaagaggtc tttatcgacg
     1261 actggggatt tatcacgggc acgtacgaaa gcgtcgcggc ggccgaatcg gcgccggttg
     1321 gatcggcccg gtgccgcgtc attaccgggg gcccgaacga ggacatggac aaactcgcca
     1381 tgatactcac ggcggctgtc gcggcggcca gaacctccgt cgttatcatg acgccctact
     1441 tcatcccgcc gcggggattg ctcgtagcct tgcagacggc agcgctgcgc ggcctgcgcg
     1501 tatcgatcgt tcttccggag aaaagcgact cccgcgtcgt acactgggcg acgcgcaacc
     1561 tgctgtggga actcctggag cgcggcgtgc gggtgtacta tcagccccct ccctttgtgc
     1621 ataccaaact ggttattgtc gacgatggct acgtgcatat cgggtccgcc aatatcgatc
     1681 ctcgcagcct gcggctcaat tttgaactga acgtggagat tttcgacaag cccctcacgg
     1741 gaaccctcac gcgggatgtg aaagccgtga tacaaaaatc ccgggccgtc acgctcgatg
     1801 agctggatgg gcggccgttc cccgtcaagc ttcgcgattc gctggcatgg cttctgtctc
     1861 cttatctgta aatggggcat caatcttctt gcccaagggg atgcactcgt gtaaaagaat
     1921 gccggcgtat caacacgcac ggctcaaaaa ccatcatcgg gcaacatgac ctgtgaggag
     1981 gctcctatgg ttgagaaaga cctcgtttac cgcggaaaat cgaaagacgt cttcaggata
     2041 ccggacggac cgcacaaggg aaaatatcga ttcgtcttta cggaccgggc gaccggttac
     2101 atggagggac gcagacccgt cttcgacccg ggatatgata tcgtcgtcgg taagatcccc
     2161 ggaaaaggcg ccatcgcgtg tcggtttgcc gcgcacttct ttcgactgat gaaagacaat
     2221 agcgtcccca cccactacat cgatacgatc ggcgaaaatg agatgatcat cgaaccggcg
     2281 gttccgctgg gcatggaggt gaaatcgcct gaatttccgg gatcagcccc actgacaaat
     2341 ctcgaattca cctggagaaa caacgcgacg ggcagcttct ggcggcggta tcccttcgtg
     2401 accccctgca aaaatgttca cagggtggtc gaggcgtgga ccaaagggga cagcgatatc
     2461 ctgatcacga tggaggcatt ggaagaaacg ggcgcgttga ccaaaagcga aatcgagcag
     2521 tccgtgaagc ttgtcaagga gatcgccgaa atcgtttccg gggaattcgc gtcaaggggc
     2581 cttcatgtca tcgacggaaa gttcgaactg ggcaggctgc gggacgggga tggaaagatc
     2641 gtgctcatcg acgaaatatc ccccgacgtc ctgcgcgtgt gccggggatt cgagccggac
     2701 agggacggaa actgcacaca cttcaaggaa tgcgccgtca cccactattc ggacggcaaa
     2761 cggaccatca aaaacaaaaa tcaggtttcg gcggccgact tcatcgacat ctttctctaa
     2821 tcggcaagaa agtcgagcgt tgttttcagt ttttcgggat aaacgtcctg gatcggtttt
     2881 tcctcacgct atctcaccga aaatatcagt aatttgcttt ttgaaaaggg aatcaaatga
     2941 aaagggatgt tttaaaaaga gacctgcgtt ttgccattat ccccaaggtg gttcaccctt
     3001 ggtttgaaga agtctaccgc ggagccatag ctcaggctga gctgttggaa aatcacctgg
     3061 gcataaaaat agaagtagaa tatcttactc cggaaacagc cgatttggct gttcagcggg
     3121 ctatttttga taaagccatc gccgcagatt acgacgggat taccatcgat ccgctcgata
     3181 agctgttcaa tatgaagtct attgcggaca ttaaaaacaa aggcattccg gtgattgttt
     3241 tcgattcacc ggcgccggta gccggcatct taagtgtcgg taacaatttt acgcagcagg
     3301 gaacggtggc agcggagcgt ctcgctaaac tgctggaaga aaaggggaag gtcgccgtta
     3361 tgcagggcta tcccagcgct ccgaatcatc atgagcgtta tgtggcgcag atagcggtct
     3421 tgaagaaata ccccgggatc gtaattgtcg acgggggcat cgataacgac gatatcgacg
     3481 tggcgaaaca acaggccctt tcggttctcg aagcgcacac cgacctcaac ggatacctgt
     3541 gttgcgatgc ttccggtccg ataggcatcg ccgcggcgat caagaaagca aatcgggttg
     3601 gcattgtgaa ggttgtcggt atggacggaa tcaaaccgat tcttttatcc atcaaggaag
     3661 gcgtgattga gtcatcggta gccaccattc ccaggatgca gggctcgatg tcgattctga
     3721 tgctatggca ggcatcgctg ggagttcaaa taccgcggaa aatcgatacg aatatagaca
     3781 tcataacgcg ggacaacgtg gacaacttcc tggctttagc gtaagtgacg gataacgcca
     3841 cccatcggtt tgtaccgttg agtcatcccc ggcgcggtca ttgccgccca tacatgaccc
     3901 ccggccaggc cgcccttgcc ccgcggccgg aattctgcta tgaagtccgc atgaatcgga
     3961 tggagatcga catcgaggcc ctgatccccc accgcgggcg gatgcgcctc atcgaagggg
     4021 tgattgccgt cgatgacgaa agggccgtga cgacggcgat cgcgtcggcg gactggcccc
     4081 tgtaccggga cggctccgtc gatgtcctcg tcacggtcga actcgtcgcc cagtcggccg
     4141 cgctcttcga agggtggaaa cggaaacagt ccggaaaagg cggcgccaag ggctggctcg
     4201 tcgggatcaa gagcgcccag tttcagcgct cccggatcgc cgttcccgcc atcctgatca
     4261 cggaggtccg gcggaattac gccctcgaaa gctatgcggt cttcgacgga accgtgcgcg
     4321 cgggggcgga actcgtcgcc gccctgagca tccaggcctt ccgtccggaa gaagaggacg
     4381 cctgaccggc gttccccgga taccccccgc ccatggaact catcccctac cgagaagaac
     4441 atcgcatctt ccgcgagacc ctgcgcaggt ttttggcccg ggagatcgtc cccgagatcg
     4501 aggcgtggga ggaggcgggc attgttccac gccgcgcctg gaagcggatg ggcgaacagg
     4561 ggttcctgtg cctggaggtt cccgaggcct atggcggggc gggggcggac ttcctgtact
     4621 cgctcatcgt cgtggaggag atgacgcgga cgaaccactg cggcctgtcc gcccgcctcc
     4681 acagtacggt cgtcgttccc tatgtcacgg ccttcgccgc ggaggcgcag aaacgccgct
     4741 acctgcccca ctgcgtttcg ggggactgca tcaccgccat tgccatgacc gaaccccagg
     4801 ccggaagcga tctggccgcc atccgcacga cggccgtgga ggacggcgac gcgatcgtcc
     4861 tgaacggaca gaagaccttc atcagcaacg ggatcaacgg ggatctgttc gtcgtggccg
     4921 cgcgggaccc ggccgtggag aagccccacg ccgccatcga cctctacctc gtggaggccg
     4981 ggacgcccgg attcgccaag ggacggcgcc tgaagaaggc gggctggcac agccaggaca
     5041 cggcggagct gttcttcacc gactgccgga ttccgcgtga gaaccgcctg ggtcagaagg
     5101 ggacgggttt cagaattctc atgcagaatc tgcagccgga gcgcctggtg ctcgcggtga
     5161 gcgccgtcgc ggcggcggag atggtcttcg agcttaccct tcgctactgt cgcgaacggt
     5221 ccgccttcgg gaagcccctc gcgaagtttc agcacatcca atttgaactg gccgagatgt
     5281 cgacggagat ccgactgggt cggaccttcg tcgacaaact ggtggcggat cacatcgagg
     5341 ggaaggccgt ggtcacggag gtgtccatgg ccaagtactg gaccaccgag atggccttcc
     5401 gggtggcgga ccgttgtctg caacttcatg gaggctatgg ctactgcgag gaatacccca
     5461 tcgcccgggc gtggcgcgat cttagggtca cccggatttt ggggggcacc aacgagatca
     5521 tgcggggcat catcgcccgg cgcctggggt tgtaagcacc gcccggccgc gacggggcgg
     5581 aatcgagaag gaaccggaga cgggaaagag ggagtggact atggaaacgc tcatggcgga
     5641 gctgaaggtc aagatcgtcg ataccctgtc gcttctggac atcgccccgg aggatatcgg
     5701 cgacgaggag cggcttgtgg gcggggacct ggggatcgat tcgatcgatg tgctggaact
     5761 cgtcctcatg ctggaaaagg attacggcgt caagatcgag agccgggagg tgggggttca
     5821 ggcctttgcc tcggttcgcg ccctggccgc cttcgtccgg gaacaccgga agaaatagga
     5881 cgcgcgtgat cgtgacggcc cggtatcgga cgggcggctt tttcccaggc tcggatccgc
     5941 acaggtgaag ggatgacgac agattccaaa ctcgatacgc tggtgctcgg ttcggggatc
     6001 ggggggcttt cgatggccgt tctcctggcc cgcctcgggt ttagggtggc ggtgatcgaa
     6061 aagaaccccc tcccgggcgg actcctgcgc agttaccggc gcggcggcct ggattgcccc
     6121 gtgggcgtcc actatttcgg cgccttcggg gagggagagc cgctccgccg gatgtgcgat
     6181 tttatgggtg tcacggaagg catcgcggcg gagcggatgg ggcagggggg acccatcgac
     6241 cgttatctgt tcgacgactt caccttcgac ctgccggaag gtctcgacgc cttcaccgga
     6301 gcgcttcacc gggcctttcc acaggacgcg caatgccttg acgccatcgc cggcaatctg
     6361 cgcgtcatgg ccgacctgca gaattccttc gcctttcttt ccgcgatgcc tcccctgatg
     6421 aacctcgacc tgttccggtc gctcgacgcc tatctctcgg gtatgggctg ttccctgccc
     6481 ctcaagagcg tgctgaacgt gtccgcgagc tgggtgggga tgtccggggc cgaatgcccc
     6541 gtgctctacc atcacctggc cctgacctcc tacctccagt cgtcgtggcg gctcaagggg
     6601 cgcggatcgg acctggccga ggccttcgtc tcccgactcg acacgctggg cggccggctg
     6661 atcctgggcg atccggcgga ggcgattctc gcgtccggag gggccgtgac cggggtcagg
     6721 cttgcctccg ggcgggtgct atcggccgac cgcgtggtgg ccgcccttca tcccaaaacc
     6781 gtcctcggga tgctcccgga aggcgccctc aagccgcttc gggtgcgacg gatccggagg
     6841 ctggccgaga cggaggggct attcgtggtg agcgccgccg tggatgcgag cgcccatccc
     6901 gccctgccgc acaacctctt ccgtctgcgt gcggaccggg acggggggct cgcggagggc
     6961 gtgttctacc agctgctcga cggcacggag gagtggaatc tcctcatgat catcacgaaa
     7021 agcctctttg cggaatggcg ccgctgggag gacacgatga cgggacggcg cggcgcggac
     7081 tacgaggcgg aaaaggccgg ccgggccgac cggatcctgc gacaggcgga ggaggttttc
     7141 ggccccttcc acagggcccg ggtcctcgac acttacaccc cgcttaccct ccgggactgg
     7201 acgggcagcc ccgagggggc cccgtacgga attctgcggt cgacgcgcca gctcccggcg
     7261 gccgcgaccc tccaccggcc gacgctggcg gggctgtatc tcgcgggtca gaacgccctg
     7321 tcccccggag tcctgggaac ggtgctgggg tcgtttcagg tggcgaggca gatgatcggt
     7381 caggaacgct tctcccgcga ggtcttggag ccgctccgct ccacccccgc ccgatgatgc
     7441 ggccgcggag gaacgagccg gaacacgcgg tgacggccgc aaggaaagaa ggacttgcca
     7501 ggtcggggaa taatcattat gatcgcccta tggaaaacgt aaagacagga tcccaatgac
     7561 gtgatgacga gagaacagat ccaaaccgca atcctggaga tcttccgccg tgagttcgag
     7621 atcgaaaatc ccgggctgga cgagaatctt cgtgaggtct acgagttcga cagcgtggac
     7681 gccatcgagc tgctcctcga gatcgagcgg tttctcggtt cggagctgac ccatgatgag
     7741 aagaaggggg cgatggagat ccggacgctg aaccaggtca tcgactatgt ggacggcctg
     7801 gccgggaaac ggggactcac gaaggacgct ccgacgcgga aggaggcgtc ctcccgccca
     7861 tgaagggacg aagagtcgtc atcacggcct gttcggctat cacacccatc ggtcacggac
     7921 gcgcggcgat cctggacagc ctccggcggg gcgtatccgg cgtcaagccg ctcagggagg
     7981 ataacctcct agcgaaatac atccactccc aggtcttcgg aaccgtggac gatccgatcg
     8041 cctacgattt caagcgcacc tatcgcaaaa cgatggggcc ggtcgcgtat tacgcctgcc
     8101 aggtggcgaa ggaggttttg gaacaggccg gcctgccgcc cgaattcgtg acctccggac
     8161 ggctcggcgt cgctttcgga tccacccacg gcagtcccac ggtgcagcgc gagatctaca
     8221 agactttttt cagcggaacc cgcgcggagt attcctccat cggcgccgtc gactacctca
     8281 aatccatggt tcacacgacg gcggtgaaca tcacgaagat gttcggcatc acgggtcggg
     8341 tgatctcctc ctccacggcc tgcacgacga gcagccagtc catcggctac ggctacgagg
     8401 cggtcaagtt cggcatgcag gaggccatgc tctgcggcgg ggcggacgag tacgacacga
     8461 cgaccgtggc cgtcttcgac aatctgctcg cctgttccac cgccttcaac gaccggccgc
     8521 agtgtacgcc gcgcccgttc gaccggcagc gcgacgggct cgtggtcggc gagggcgccg
     8581 gggcggtgct cctggaggag tatgagcacg cgaagcggcg gggggcggag attctcggag
     8641 aggtgatcgg gtttgcctgc aacaacaacg gcggcgatct catcctgccg aacctggccg
     8701 ggatccgtgc caccctccaa tcgagcctgg aagtcgcgga gctgaacgcg gacgacgtcg
     8761 acttcgtcag tgcgcatgcc acggcaaccc ggatggggga cgtgatcgaa gcccaggcga
     8821 tccacgccgt ctacggcgac aatccgttcg tgacgggctt caagggctac atggggcaca
     8881 ccatggggtc ctgcggtgcg atcgagacga tcctcagcct ttaccagatg caggaggggt
     8941 tcatcgcccc gacgctcaac cttgaagaac cggacgagcg ctgcgcgatg atccgtcata
     9001 ccctgaagct ggaggagcgg cccgtaacgg tggccgccgt ccagaatttc gccttcggcg
     9061 gcgtcaacac ctccctcctg atcaaacggt ttgcctgacc cccatggtga ccttcccccg
     9121 cctcttcaag ccgctggtcg actgggccgt gacgatgatt ctgtggaccc attttacgct
     9181 ggggttctgc gttttcttcg cgccccgcta cgtctgggcg tgctttcgcg cgcgggaccg
     9241 cgaggccgcc tttcagcgcc tgaaccaccg ctacttccgg agcttcttcc gcctggtcca
     9301 atggatcacg ccgcggctca gcctgcggat cgacgcggag gtccggaccg tccgctccgc
     9361 cgtcatcgtc tgcaatcacc tgtcctatct ggatcccatc ctactcatct ccctgttcga
     9421 aaagcagaag acgatcgtca agagcgtctt cttccgggtg ccgattttcg gctgggtgct
     9481 ccgccgctcg ggatacattc cctcgaagac cgggggcgac tacacgtcgc tcatgatccg
     9541 gaacatcgag accatccggg ggtatctcga cgccggcggc aatctcttca tctttccaga
     9601 agggacccgg agccggacgg gaaaactggg gccctttaac aagggcgcct tcagcattgc
     9661 ccgctactgc cgggcgccgg tcgtgtgtct cgcgatccgc cactcggacc gcctgttcat
     9721 cccgggacgg ttccgtttca atacctgcgt ttccaacgtc ctgtccgtgg cgcggatcgg
     9781 gtcgttcgac cccgattacg gcgaccccgc cttccgcctc ggcgacctga tccgcgagat
     9841 tcgcacgctt tacgaagcgc ggcaggcgca ggattcggat tgacttttcc ccggattgtt
     9901 ctaaagatcg accatggaaa acataacgga aaacgagcgg gtcgtcatta cggggatggg
     9961 caccgtcaac gcgatcgcgg gagatccctt ggaattcgcc gccgctctga aggacggctc
    10021 gtgcgggatc ggtccggtga cccggttcga tacgacggcg tttcgcacgc acaacggggc
    10081 cgaagtcaag gatttccacc cccgcgccct cctgcctccg gaattcagcc tgaagcgcgt
    10141 ctcccgcgcc gacgccttcg cgctcgtggc ggccttccag gccctctcgc aggcggggct
    10201 ctatccggtt cccgagtccc tgcgcgaacg gaccgggacc gtcatcggcg gtggggccgg
    10261 cggcctgccg gaagcggagg cattttacca ggattacctg cgccgcgggg gcggacgggg
    10321 gagattttcg cgtcttgcga ccgtcgcctg tgccgcccac gccaacgcga ttgcgacgca
    10381 attcggcctc tacggtccga aggccacgat catgacggcc tgctcctccg gcgcgaccgc
    10441 gatcggtctg ggccgtgacc tcatccggac cgacgccgcc gaggcgatga tcgtgggcgg
    10501 ggcggagccg ctctgccgcg tgaccttcgc ttctttcaac gccctccagg ccgtcgatcc
    10561 cgggtattgc cggccgttct ccggaaaccg cgccgggctc accctgggcg agggggcggg
    10621 gattctgatc ctcgaatccc ttgcgggcgc gcgggcgcgc ggggcggcga ttctgggcga
    10681 ggtgctcggt tacggcgtga cctgcgactc ccaccacatg accgccccgg acaccggcgc
    10741 ctccggggcg gttcgtgcga tccgggcggc cttggccgac gcggggatcc cgccggatgc
    10801 ggtcgactac atcaatgccc acggtacggc gacgcccccc aacgacctga tggagacgcg
    10861 ggcgatcaag gcggtcttcg gaccgcgcgc gggccggatc ccggtcagtt cgacgaaatc
    10921 gatgcacggc cataccctgg gcgcctccgg ggcgctcgag gcggtcgtct cggtcctcgc
    10981 catcacggag aggttcatcc cgcccacgat ccactgcgag acgcccgatc cggagtgcga
    11041 cctggattat gtgacgacgg gcgcccggtc cgggacgccc gggattgttc tgtcaaattc
    11101 ctttgccttc ggcgggaaca acacggcggt ggtcttcggg tgctggcggg acgaggggag
    11161 ggggcgtgga taggcaggtc gtccttaccg gcatcggcat ggtcacgccc ttgggggtgg
    11221 ggaaggccga gttccgcgat gccctgtggc gcggcgaaac gggcattcgg gagatttccg
    11281 ccttttccac cgccggcttg ggctcgcacc tcggggcgga ggtccgcgac ttcgttcccc
    11341 gggacttcgt cgccctcaaa accctgcgca agatggatcg cctctcggcg atgacgacgg
    11401 cctccgtccg gatggccctg gacgatgccg ggctcgccgt gaccgacgcg aaccgcgacc
    11461 gcatcgggat cgtcatggga acggccttcg gaaacacgga cctcaaggtt cagagcgccc
    11521 gggttctctt cacggaggga cccggccggg tgagtccgat gcatgtaccc aacacggtga
    11581 tgaacgcccc cgccggacat gcctccatcg aactggggtt ccgcggcgtc aacacgacaa
    11641 tcaaccacca ggccgtctcc ggcgaggcgg cgattgccta cggggcgatg gaggtccgtc
    11701 gcggcgtggc ggacgtgatc ctggcgggcg gcgcggacct gctgtcgccc ttcttctacg
    11761 aggctttgtg ccggtttcgc gccctctcgc cgacggacaa cggcgtggag gcggcgcggc
    11821 cgtacgatct gcggcgcaac gggcccgtca tgggcgaagg gtgcggaatc gtctgtctcg
    11881 aggcgaggga gcacgcttta gcgcgtgggg cggatcccta ttgcgaactg gccggctggg
    11941 ggcttgccgc ctcgccggca tcgcccacgg gttggccgga ttccccccgc ggccttcttg
    12001 tcgccctccg gcgggcgctc gacatggcag gggtcgtccc tgcgggggct ggttccgccg
    12061 ggtccgacgg gggaccgcaa gacgaccgcc gccggcccgg tggcggcgtg cccaccggga
    12121 tcgactgcat ccagggggcg ggaaacggcg ggcgcgaccc cgacgcgctc gaggcaatgg
    12181 cctatcggga ccttttcgga gggtccgaag ggatgcccct catcacctcc ctcaaggggg
    12241 cgacggggga gatcttcgcc tccggcggca tccgggcggc ggccctggcc ctgtcgatcc
    12301 gggagggcgt ggttccgccg gtcgtcggcc tggcggcgcc gctgatgccg ttgccgttcg
    12361 tgacggggga ggctcgccgc cggtcggtcc gacgtgcgct cctgagcggc ctgtccttcg
    12421 gcgggaccta cgcgggcctc gtcttcagtg cggtgtaaaa acgttggcgc cacgccgccg
    12481 ccatcggccg tttcccggcg catcgcttcc cgcgggaggt gtccccaaga aacgtccgtc
    12541 gcgccctcca agaacgcatg gaagaaaccc cgagaagtgc tagttggcct ccgcgattcg
    12601 acaccgcagg cacgttactt ctgaagaatg aacactatct ggtcgagacg cggaaccttt
    12661 aaatattgct tgcctctttc gaaaaaaagc tttaggaatc aaacggagtc aaaaaaaccc
    12721 cataacagga ggacacatca tggctgatct gctcgaatta gcaacagaca tcgtcattgc
    12781 ccatgcttca acgaccgaaa tgacgtcgga tgacctgatg agcgaaatca aggccgttta
    12841 tgcgacgttg aagggcctcg aacagggcga agcgggagat gtgcccgcga agccggccct
    12901 gacccgtagg caggccttca agaaggatga ggtcggctgc atgatttgcg gcaagaccgg
    12961 cctgaccacc ctgaagcgcc atctcgccac cgcccatgag atgaagcccg gccagtaccg
    13021 gaagcggttc aacatcccca aggaccagcc cttggccgct acgaactatg tcgaaaagaa
    13081 acgtcagatc gccctggacc ggggattggg aaagaatctc gccaaggccc gggcggccaa
    13141 gacgacgaag gtgaaaaagg cgaagaaatg agttgggggc aggtgaatgt cggcgagtgg
    13201 atcaccaagc gggcgttgac ccatccggac aggcccttcc tctgcgagga ggggatgcgg
    13261 aactacacga accgtgagtt caacgagcgg gtgaaccgga tagcgcacgc gctcgcggcc
    13321 tggggtgccg gccggggcga acgtgtggcc gttctcatgg ccaacgcttc ggagttcctc
    13381 gagattttct tcgcctgcgc caagaccggg gcgatcatga cgcccctcaa cgtacgcttg
    13441 gcggtgccgg aactcgtgta cattctgaag gactgtgccc cgcgggttct cgtctacggg
    13501 gcggattttg ccgacaaggt cgccgaaatc aaggcgctcc tgacgcaact tccattctgc
    13561 gtccgtcacg ggggcgatac cggtcaagat cccgacttgg cccgggcggt ggcggacttc
    13621 cccgatgcag aacccgcgcc ctccccggag cttgccttgg acgatcccct gttcaccatg
    13681 tacacctcgg ggaccactgg cgaccccaag ggggcggtcc tgacgcacgg aaacgtgctc
    13741 ttcgccgcga tcaacacggt gatgggctac gggatcaacc gctcctacaa atccctcgtg
    13801 gtcgcgccac tcttccatat cggcgccttg ggcgcttcgg ccctgccgat cctctatgcc
    13861 ggcggttccc tctatatcaa ggggttctac aacgcggctg aggtcctgga cctgatcgtg
    13921 cgggaaaaga tcaactacct gttcgccgtt cccgtcatgt ttcagctcct cgcccagaca
    13981 gagcagtggg cgcaggctga cttctcgcac gtccactttt tcatcgccgg gggcgcgccc
    14041 atgcccctgc ccatgatcca gaagtaccag accgaaaagg gcgtctcctt tgcccagggc
    14101 tacggcatga cggaggcctt ccggctcacc tccctggacc tcgaggacgc cgtccgcaag
    14161 gccggttcgg tcggcaagga ggtgctgcac gtccggcttc ggatcgtcga tgaacaggac
    14221 cgtgacgtgc cgcccggtga ggcgggggag atcgtcgtga aaggccccaa cgtcttcgcc
    14281 ggctactgga acaagcccgc ggaaacggag tccgcgatgc gggacgggtg gttccacacc
    14341 ggggacatgg gacggcggga cgaggagggg ttcgtctata tcgtcggccg gaaggtagag
    14401 atgatcatca gctccgggga gaacatctac ccggcggagg tggagcgggt tatccaggcc
    14461 ctgccggagg tccgagaggc ggccgttgtc gggatgccgg atcggaagaa gggggaggtt
    14521 gtggccgcct tcgtcctgct caaggaaggc gcggcactca acgcagaggc gctcttggag
    14581 gcgatccgcg acaagatcgc ctatttcaag cttcccagga aaatcatctt cgttggggat
    14641 tttccgcgca acaccgcggg gaagatcttg aagaaggacc tgaaggaaaa ggtgtccgga
    14701 agcgaaggaa gatatcgtgt cagggagggg ttatgagcga tcggcagacg gcgatcgtca
    14761 cgggcggcag ccgcggcatc ggccgggcca ttgccgtgga actggcgtcc ggcggctatg
    14821 acgtggtcat cacctacagg gcgaatgagg cggccgcgga ggagacgctg aagatgatcg
    14881 cggcggcggg cggccgcggc gaggcgctcc ggttcgacgt cggcgaccct gacgcggcgg
    14941 acgcggcgct gggcgacctg ctcgtccgga tggagcgcgt cgacgtcttg gtgaacaacg
    15001 ccggaatcac cgctgacggc ctgttcctga tgatgccccg gcacgactgg gacgccgtga
    15061 tcaaaaccac gctgggcggc ttctataaca tgaccaaacc ggtcctgcgc aagatgctgc
    15121 gcaagaagcg cggcgccatc gtctcgatgg cttccgtctc ggccctggtc ggcaaccggg
    15181 ggcaggcgaa ctacgcggcg gccaaggcgg ggctgatcgc cgcgagccgc gcgctcgcct
    15241 cggaggtggc tcgactcggc gtccgggtga atgtcgtcgc gccgggtctg atcgccacgg
    15301 acatgatcaa ggatgcgccg gtggagaaca tcaaggaaat gatccccatg gggcgtatcg
    15361 gtcggcccga agaggtggca aaggtggtcc gctttctctg ttccgacgac gcctcctaca
    15421 tcacgggcca agtcatttcc gtcaacgggg ggatgttttg aggttccgct tgccgagggg
    15481 aatcgcacgg cgcgggcgct ttggccttct cgggctgctc atgctcctac cgctcctgac
    15541 ggcctgggcg gacagctggg agggattgcg cgaggccgcg caggggatca cgtcgatcga
    15601 ggcccggttc gtccagaaaa agaccctgcc catgctgacc cggccactcc tctcggaggg
    15661 acggttctcc taccaggcgc ccgaccggct ccgctgggaa tatgaccgtc ccgtccggag
    15721 cgtcctcatc gtgaacggcc gggacgtcaa gcgctaccgc aaggagcggg acggctggcg
    15781 cgaggagacg ggcgccgccc tgtcggtcat gcagacggtc atggaggaga tcctccactg
    15841 gcagcagggg cgcttcgatg cgaaccccca tttccaggcg acgctcgaga cgggctccaa
    15901 accaaaggtg acgctcaccc ccaaggatcc tgcctggcgg aagatgctcc gccggatcga
    15961 actgaccctg tcgcgaaagc agtcgggcgt gatgcagagc gtccgggtgg tggaggacga
    16021 acgctccttc acccagatcg aattcggcca ggtggcggtc aaccggcccc tgccggcggc
    16081 ccggttcagg gacatcgaat gaaggccttc ggcgccacct tcctcctcgg cctcgccctc
    16141 ctggcggtcg ggggctgcgc tgcgctgcca ccgttgacac ccctcgatcc ctcccaaaag
    16201 cccacggttc tctccgggtg tcgggagcct ttcctcaaag agaaataccg cctcgtccat
    16261 gtcctgacgg ccacgctgcc cggcggctcg gaaagcgccg ccatcggtgt cctggtggcg
    16321 gatcccggtc cgcggcgctt tcagacagtc atcatgacgc tggagggatg ggtcctgttc
    16381 gatgcgaccg taggggaggc gctcgtcgtg aatcgggccg tgcccccctt cgacggccgg
    16441 gcgtttgtcg aggcgctggt ggaggacatc cgcctggcga tgtttccacc ggagggggag
    16501 ccgatcgcct ggggaagggg agagggtggt gcggcggttt gccgcttcgc gcgccccgac
    16561 ggcacacata tggacgttct gcgcaccggc gaggacgccg cggaagtgcg gctgtacgga
    16621 actggacaag gactccgaaa aagggtaagg atcggatcgt ggagcggatc gggtcccgcc
    16681 gggacactcg agatcgaaag ccggacctgg ccctcctatg ccctgtatct gagactcctc
    16741 gaacaggagc gtctggagac ggagaagacc ttgccatgaa atttcgactg atctaccccc
    16801 gctggaagaa actggaccgg cagacggaat tccacctgcc gccccacgga ccggtcgtct
    16861 tcgccgcgac cctgccggac gactgggagg tggagttcat cgacgagaac gtgcagcccc
    16921 tcccggagaa cagcgacgcg gacgtggtcg gcctttccat gatgctgacg agccagatga
    16981 agcggggctg ggagatcgcc gatgattatc gccgccgggg cgtcaaggtg atctgcgggg
    17041 gcatcgggac gatgctccat gccgaagaga cgatgcggca cgcggacgcc gtttttctcg
    17101 gcgaggcgga aggacggatg gcgggggtcc tggacgactg gcggcacgac cgtctgaagc
    17161 ccgtgtacga ctactttcac gaccacccgc ccacggaggc gatcggcacg gcccggcgcg
    17221 agatcctcaa ccgggatttc tacaattaca aggggatcca gatggtggac ctcgttcacg
    17281 cctcccgtgg ctgccggttc aattgctacc cctgctgtgt gagctatctg ggcggacgca
    17341 agttccgtcc ccggccggtg gacaaggtcg ttgccgagat ggccgcgatc gacaacaacc
    17401 gcctgttcat cgtggacaat tccctggcgc aggaccgcaa ctgggagatc gagctcttcc
    17461 gggagatgat cccgctcaag aagaagtggt gctgtcaccc gatcgaggag gacgacacgg
    17521 tcctcgacct cgccgcgcag gccggtgcct ggtacgtcta tcaggccgtg ttcgacatgt
    17581 cggaccacat ccgcaggcgc atccgccgct accacgacca cggcatcgcc gtcgagggga
    17641 cgatcctact aggactggac gaccacacgg aggactacat caagcggttc atcgacttcc
    17701 tcatggagat cgagctggat ctggccgagt tcaccgtgct cacgcccttt ccccacacgc
    17761 gcaccttcga cgacttcgag cgcgaggggc gtatcctcac ccacgactgg gacgcctaca
    17821 ccgccgaccg ggtcgtcttc cagccccggc acatgagtcc cgagcggctc caggagctgt
    17881 acgccgaggc ctgggacact ttctatcggg acgagccgca gaactacaag atgttcaagc
    17941 tctttaaaac ggtggtggaa cgcgagaagg cggacgggac cttccgtccg cgccggcggg
    18001 agatggcgac ccgccgtttc ggccgccggg gcgggggggg aggggacacg cccgctgcgg
    18061 cgaattaacg cttgacaagc ggcgtttttt aaaagaaatg ctatcgccgc gtgaaggatg
    18121 atgaaggtaa tctaacggat gtcaaagaaa aaggacggaa tcaaaaacgt gttgatcgac
    18181 gggacgacgg tgtccctgga tgggattatc gccgtggcca ggagggaggc gtcggtgcgc
    18241 ctgagtgaac gccccgcctt cagggagcgc ctggagcgtt cccgccgcgg cctcctggcc
    18301 gccatcgcgg cggacgtccc ggtttacggc gtgaataccg ggtatggaaa atcctgcggg
    18361 aatcgcctga agaaaaacgc tgttctcgga aacggcggca acatgatgcg tttccacggg
    18421 tgtggaaccg gggaaacgct gggggtcgag gagacgcggg cggcaatgct ctgccggctg
    18481 ctctgcctgg cgcgcggcta ttcgggggtg tcctacgcct tgctggagca gttggccgcc
    18541 tttctcaacc tgggcctgac ccccgtcgtt cccagtgagg gctccgtcgg ggcctccggg
    18601 gatttgacgc cgctctccta catcctggcc gcactcgcgg gcgagcgcga cgtggtttac
    18661 gccgggaagc gcatgccggc gcggcgcgcc ctgaaacggg cggggctcaa accgcacctc
    18721 ttcggcgcga aggagccgct cgcgatgatc aacgggacct cggtcatgac cgggatcgcc
    18781 gctgtcgtcc tcgaccgggc ggattatctt ttgcgggccg ccgtcgatgc gaccgccctc
    18841 agcgtccacg ccctgcaggg caaggcgcac cactaccacc ccgtcatcgg ggaggccaaa
    18901 ccctttccgg gacagattca cgtggcggac cggcttcgga aacatcttca gacgagggct
    18961 cccgaggcgc aactggagtg cgaggggccg gaaacccttc aggatcccta ttccctgcgc
    19021 tgcgccccgc aggttgtcgg ggtcctcgac gacgccctgg cctggatccg gccctgggtg
    19081 gagatcgagg cgaacagcgc caacgacaac cccctcttcg ccgaaggggt ggagcatccc
    19141 ctcatggggg gaaatttcta cggcgggcac ctcgcctttg cgatggacgg cctgaaggcc
    19201 gctctcgcgt cggtggccga catggcggac cggcaagtgg tgctcctcgt caatccacaa
    19261 gtcaaccgcg ggctcccggg cgacctcgtg cgcgtggacg gccccgagcg gttccgacac
    19321 cacggcttca aggccatgtc catcaccgcc tccgcgctga cggcggaggc cctcaagcag
    19381 acgatgcccg cggcctcctt ctcccgctcc agcgaatccc acaaccagga caaggtcagc
    19441 atggggacca tcgccgcgcg cgacgcggac cgcgtctgca cgctcacgga gcgggtcgtg
    19501 gcgattcatc tcctggcggc ggcgcaggcg tgcgagatcc gggggcgact cggggaccgt
    19561 cccgctctgg cgaagaaggt cgcggccatc cgcaaactcg cccaaccgac ctgggaggac
    19621 cggccgatgg acgcggacat cgaatccgtg gcccgggcga tttcacgggg ggagatggcg
    19681 gatggataac acggagcgcc ccagctacga ggtccggctc aaggtccctt ttttcgatct
    19741 cgatcccatg cagatcgtct ggcacggcca ctacctcaag tatttcgaca tcgcccgctc
    19801 ggagcttttc gatcacctgg gcgtcgacct gttcgaatat cacgaccgga ccggctacat
    19861 ctt
//
LOCUS       DAOOLJ010000031        18867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_2999_length_18867_cov_17.444238, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000031 DAOOLJ010000000
VERSION     DAOOLJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 18867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18867
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2999_length_18867_cov_17.444238"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2651
                     /locus_tag="PLW16_06540"
     CDS             <1..2651
                     /locus_tag="PLW16_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HPB70598.1"
                     /translation="PEIIALYKGKFKIDPYMQEHMPRTVAVLPFYDLSKSGKGFDAVR
                     KGFYNHFSAVPYQDMELYKVDALLAKAGLTDPDILYKTPYEELGKILGVDAIVLGEIS
                     DFDKLFAILYSHVAVGAEVRMYDAKTGHFLWSGKHTVRIHEGGVSTTPIGIIATIVAT
                     SLNMRDIQLLRACDDLFRDMVKTIPAPSLAEALRPPTITLLTQDTKGLPKKAGDEVRI
                     VIQGTPKMRARFDIGDYRRRIDMQEIEPGGYLGVYKVLPGDNVQKATITGYLEDDARN
                     MAQWVDAVGSVTLDTTPPDIPKNYKSVGRNGLVLLNWEKSVAPDLVGYRLYRSETPLT
                     GFTEIARTELNEHRDAGLTNDRKYYFQISSVDQAGNESERSEVIVGMPVTPGPTPVSG
                     AIEADTIWYSGASPYVIENSVIVRDKARLTIEPGTEIRSGGGGITVEGRLLAQGDGEH
                     IITFDAEEGKTWEGITFLNVKEKENVLRNVRIRKARIGVLCMAASPRIEASEFVENGT
                     AVRISGAFSKPNLVQNTLHRQKEAAVVVEAGAQPLLAENRIRDNEGKGIVVQASSPVI
                     TRNVIAQNRGVGIDLKTSPARISENNLTDNRPFDMTAEMTGEAVNASGNWWGTAQGLE
                     VLKKIRGRVDVATVLSAAYPDGKPVALPILSQTLDGAITADGFLTVSNSPYRVQKDVT
                     VDGGATLYVEPGVEIRFDQNTSLIMADGGIVAKGTPDRPIVFTASGTTPAPGFYTNAV
                     RFTKKTKVNSALAYCEVKFASTALDVYTGAPEISHCLIAQTSQGGIFCRNDAAPRISY
                     STFTANAGEGAIKSVGMSNPSIHRNNFIDNPVALQIFSSIYIDARENWWGKAPPDVSL
                     IWGDLDRNINIKPWLEKPEDRAFRGR"
     gene            2784..3695
                     /locus_tag="PLW16_06545"
     CDS             2784..3695
                     /locus_tag="PLW16_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70599.1"
                     /translation="MKSKWVLTIGILLAGLFLYAGVGLAADTVSMPEWTQIIEQMAGK
                     GKINWSEGYIEAVGIGAPPERFIGKPQARPLALRAARVDAYRNLLELTKGVRVDSMTV
                     VKDFAVESDVINTQVEGVVKDAHLVKQDYLSDGTVEITLRMPLAGGFSQVIIPRLMQK
                     RSVPPEPAAPLAPPAAAPPEPAAPAPAAPPEVFTGLVIDARGLQARPAMSPKILDESG
                     KEVYGSLNVDREYAIQQGMSGYARDLNAAQSNARVTNNPVSLKGLRTEGPGRSDIVIS
                     NADANKIRGAAENLSFLKQCRVMIVLD"
     gene            3807..5588
                     /gene="mutL"
                     /locus_tag="PLW16_06550"
     CDS             3807..5588
                     /gene="mutL"
                     /locus_tag="PLW16_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPB70600.1"
                     /translation="MGKITVLPEELTNRIAAGEVVERPASIVKELLENALDAGAETVG
                     IELRQGGCGAIRVTDDGGGMAAADVPLAFSRFATSKIASFEDIYRIRSFGFRGEALPS
                     IAAVSRVTLITRRRDRPDGTRLIVEAGQIIEVTDCGCPSGTSILVENIFDPVPVRKKF
                     LKRESTELSACLEAITRVALPHRHVRVRVQANGRSVMSLPATEGDLERLGLLLGEEAA
                     GHLLPFSGFRETASLSGFCSRPAWTRSDGKQIFCYVNGRFVRDPLLQHAVMTAYRRLI
                     EARRYPACILYLDVAPEEVDVNVHPAKAEVRFRQPRDIYALIVESLVAILSGSTAVPS
                     APLADIAREPLYQARIEEAMKRYTISSGRSKRLFAGLPAAPAVRPDRDAGFPTPPTTA
                     APFPEAAIRFTEGTYLGQVLSTYLVFAHPAGILLMDQHAAHERVLFERLRTSGTAGKV
                     VTQQLLLPEVISLRAGEFQLALSLQALWDELGFAIEPFGDTSIAVKAVPALVPNLDPR
                     QTVRDLLSELSELPATAGLREQRDRLHAVLACKGAVKAHQELTEAEVGALCRELEAIS
                     FSSTCPHGRPAYVLIDERDLKRMFRRT"
     gene            5613..6551
                     /gene="miaA"
                     /locus_tag="PLW16_06555"
     CDS             5613..6551
                     /gene="miaA"
                     /locus_tag="PLW16_06555"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPB70601.1"
                     /translation="MTSGDRPKLIVILGPTAVGKSSLALELAQAYGGEIISADSMQVY
                     RGMDIGTAKPGREARARVPHHLIDVVDPDEPFNAALFVEQAGQVIDRLHRLGKPVFVV
                     GGTGLYIRALLGGLVGGPGPDEALRAQWRAVRDEKGVSHLYEQLRRLDPRAAGRIHPH
                     DAVRIVRALEVLALTGESIVGKQAEHRFAERRYRSLKIGLRLEREPLFARIEARVDAM
                     IAAGLVDEVAGLAARGYDASLKPMQSLGYRHIGGWLRGEADLSNAVRRLKRDTRHYAK
                     RQQTWFAPDPEIRWFSPGDTAGVEACLRPFLNREIP"
     gene            6663..7880
                     /locus_tag="PLW16_06560"
     CDS             6663..7880
                     /locus_tag="PLW16_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70602.1"
                     /translation="MVRLFKNRNLTTVLVLVAAALLLVSCGPKARVATSELDTPEHHF
                     YTGMKLLDQGRYVDSQQSFNLAIQLDPKYSKAYAGSGLANAYQRDFSAAFAMMKKAGK
                     YAKTEEEKVFVHVGYIRINTLSAADCLRSGIRTGVVCDPPSDWLDTAKGEFDDAVLID
                     PKSSAAYYFMGIAYKTALDINQAASMFSKVLDLKGDYVREADDQWRLVQKIQRAMPGT
                     ITGKKIAFVEQITRADAAALFMEELKIDVLYQKRTLKTFDTSFKDPEKAKESGPAPKT
                     ANDIATHPLKADIEGIIQLGVRGLEVFPDGAFHPNDLIDRASYAMMIEDILMKVSGDS
                     TLATKFIGETASSFPDLRTDLPYYNAVRVVTSRGIMEPKDLASGEFGPLGPVGGADSL
                     LIIRKFSDILKIY"
     gene            8073..10730
                     /locus_tag="PLW16_06565"
     CDS             8073..10730
                     /locus_tag="PLW16_06565"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPB70603.1"
                     /translation="MGGGTLSKTYEPRDSEAKWYAYWLEKGFFHAVDDGARPPFAIVI
                     PPPNVTGMLHMGHALNNVLQDIIVRFKRMQGYNALWMPGMDHAGIATQNVVEQELARE
                     GLTRHDLGREKFVERVWEWKAKYGGVIINQLKRLGCSCDWERERFTMDEGLSRAVREV
                     FVRLYNDGLIYQGDYIVNWCPRCHTAISDLEVEYKQEPGFLWNIRYPFADGNGEIVVA
                     TTRPETMLGDTAVAVNPEDDRYRALVGKRVVLPLVNKEIPIIADDYVTLDFGSGAVKI
                     TPACDPNDFAMAGRHDLEIIKIMDGSGVINANGGSYEGQDRYECRRNVVRDLEEQGYL
                     VGTEPYVHNIGQCYRCKTDIEPMVSKQWFVKIQPLARAATAAVVKGETRIVPSMWEST
                     YYEWMDNIRDWCISRQIWWGHRIPVWTCGGCGRMIVATQDPETCPDCEGRDLRQEEDV
                     LDTWFSSALWPFSTLGWPDGTAALKTFYPTSLLVTGFDILFFWVARMMMMGLYVMKDV
                     PFRDVYLHALVRDEKGDKMSKSKGNSIDPLEMIDKYGTDAFRFTLAAFSAQGRDVRMS
                     EERIEGYKYFVNKIWNATKFSLMNLKSEPKGTETVPPQDLSLADRWIRSRLNRTTAEV
                     IRGLDEYKFNEAAAAVYQFVWHELCDWYLELIKPVLYGSEDNAAVVAARRTLRRTLQT
                     SLKLLHPFMPFLTEEIWQTLVADGTSIMVSEFPRVDETPADPEAEARMGLVMDVISAI
                     RNIRGEVNIAPSKKVNVRIFAPEAEGAAVVAAERGHIVNLANLQSLEIDRTLEEPKGA
                     ATGVVGGLRIFVLLEGVVDIGAERARLQKELAKVNRDLEGVARKLANRDFLARAAAAV
                     VQKEERKHRDLTDKHQALEAALKRFADLG"
     gene            10744..11586
                     /gene="nadC"
                     /locus_tag="PLW16_06570"
     CDS             10744..11586
                     /gene="nadC"
                     /locus_tag="PLW16_06570"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPB70604.1"
                     /translation="MLPKTVLDPILRQALAEDIGPGDVTTEAILKGDERGRACAIAKS
                     DLVVAGGEVFREVFTLMDASLEVVLSRRDGDRARPGDSLAELNGRLDSILTAERTALN
                     FFQRLCGIATLTRRFVEEIDGTGARILDTRKTAPGLRLLDKYAVRVGGGRNHRFALFD
                     GVLIKDNHIAAAGGIAAAVAKVRQTVAHLVKIEVECKNAVEVGEALAAGADAVMLDNM
                     DLPAMREAVALVRGRVPLEASGNVTLANVRSVAETGVDFISVGALTHSVAAADISLGI
                     QQGT"
     gene            11583..12377
                     /locus_tag="PLW16_06575"
     CDS             11583..12377
                     /locus_tag="PLW16_06575"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPB70605.1"
                     /translation="MSPSFSAASLSERLAGVGLARHILFYAELGSTNEEAFDLARQGA
                     PEGTVVVADRQTQGRGRMQRVWVSPPGRNLYLSLVLRPDIAPEQAPQLSIVAGVAAAE
                     ALVGLCPGVTLKWPNDVLAGGRKLCGILAEMKASAGRLDFVILGIGVNVNLLREECPP
                     EIAGIATSLRDETGGPLSRVEVAAVFLRSLEAAYRTYCREGFPPLRTRWLERTDMAGK
                     PVRVMAGTDVYEGRMAGLDDAGGLLLEEKTGGIRAVMAGDASVVKG"
     gene            12382..13152
                     /locus_tag="PLW16_06580"
     CDS             12382..13152
                     /locus_tag="PLW16_06580"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB70606.1"
                     /translation="MLLVVDVGNTNTVLGLFDGDKLIHDWRIRTEVDHTVDEYGMLIY
                     NLYKSGRVGQREICAIIISCVVPPMLNILEPLCLKYFNLKPIIVGPGIKTGMPIYYDN
                     PKEVGADRIVNAVAAFEKYKRELIIVDFGTATTFDHVTVKGEYMGGCIAPGIMISSEA
                     LFLRAAKLPRVELSRPKAIIAKDTVSSMQAGIMFGYAGLVDGIVGRMKAEAKSKPYVV
                     ATGGLAKIVAAETKTIDTVDEMLTLEGLRIIYLRNAAP"
     gene            13211..13831
                     /gene="coaE"
                     /locus_tag="PLW16_06585"
     CDS             13211..13831
                     /gene="coaE"
                     /locus_tag="PLW16_06585"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPB70607.1"
                     /translation="MLNVGLTGGIACGKTTVAEMLVARGAHLIDFDALAHEVEAPDRP
                     AWRGIVSAFGEGVLNADRTIDRPKLGAIVFEDKAKLETLNGIVHPAVFDEWRQRMDAI
                     MHNDPRSIILSDVPLLFEVGMAPLFDLVALVYVSPAQQVERLMKRNGFTAEEARARLA
                     AQMSIDEKVRLADVVIDNQGTVEETRRGIAAVWADFVRREEAKRMS"
     gene            13932..14546
                     /locus_tag="PLW16_06590"
     CDS             13932..14546
                     /locus_tag="PLW16_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPB70608.1"
                     /translation="MIHKNVKTDFSPAALTPQIDPATYVHPLAAVIGNVILGKGIMVS
                     PFASIRGDEGQPLHVGDESNVQDGVVIHALETELNGAPVEKNLREVDGGKYAVYVGRR
                     VSLAHQVQVHGPAVILDDTFVGMKSLVFKSVVGKQCVVEPGCILMGVTVAEKRYVPAG
                     SVVKTQAQADQLPAITDDYPMKDMNKGVVHVNTALARGYLAASE"
     gene            complement(14615..14944)
                     /locus_tag="PLW16_06595"
     CDS             complement(14615..14944)
                     /locus_tag="PLW16_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPB70609.1"
                     /translation="MAQEKTFITISTGDHEDLFFKGQEQEKIRKLREKAARESDQKYR
                     EEHKNHCFRCGTPSLAEISHGSVKIDVCINEDCGAVHLDPGELEAILRDRDSIEKVRK
                     AVFSIFK"
     gene            complement(15131..15820)
                     /gene="aqpZ"
                     /locus_tag="PLW16_06600"
     CDS             complement(15131..15820)
                     /gene="aqpZ"
                     /locus_tag="PLW16_06600"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HPB70610.1"
                     /translation="MKAFGAEFFGTFWLVLGGCGSAVLAATFPDVGIGLLGVALAFGL
                     TVLTMAFAIGHISGCHLNPAVSIGLWAGGRFPAKGLVPYIIAQVLGGIVAGGVLYLIA
                     TGKAGFDVAAGFASNGYGEHSPGGYSLLAALVTEVVMTMMFLIVILGATDKRAPQGLA
                     PIAIGLCLTLIHLISIPVTNTSVNPARSTGVALYVGGWAVAQLWLFWVAPIVGAILGA
                     TVYRWIGRTDP"
     gene            complement(16043..16612)
                     /locus_tag="PLW16_06605"
     CDS             complement(16043..16612)
                     /locus_tag="PLW16_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HPB70611.1"
                     /translation="MPPKSPKIPAAVYQFKITLKGSRPPIWRRVWVPDHFSLYKFHRV
                     IQLAMGWEDGHLHQFIVDGRYYNIPHPDDWEPGIDERRVKLEKLAPSLNSRFIYEYDF
                     GDGWEHVILVEKIIPPDAGEVYPKCIQGKGACPPEDVGGIWGYQRFLQAIRDPNHEEH
                     DSYLEWVGGEFDPKAFDIEAMNRALRKVK"
     gene            16979..17818
                     /locus_tag="PLW16_06610"
     CDS             16979..17818
                     /locus_tag="PLW16_06610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70612.1"
                     /translation="MKTGAGQGRYADQNVFITGGSSGIGLATAEAFAGEGAHVAIFAR
                     NPGRIEEAVARIRRARRRGDERVAGFPLDVADPTRVREVMTQAIREFGAPHVLVNDAG
                     VTYTDYFENITYEQFDEILHTNLYGMWNTITTLLPAMKAHGGLIVNVSSIVGFLGVFG
                     YAAYAASKFGIVGLSETLRMELKRYGIRVCVLCPPDTDTPQWHGENRTKPRETWALAG
                     NAKVMKPEAVAQVLLAGIGKGRFMIIPGTEGKFIYIMKRHFPALVAWIMDRDVRKAAA
                     KGV"
     gene            17841..18848
                     /locus_tag="PLW16_06615"
     CDS             17841..18848
                     /locus_tag="PLW16_06615"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB70613.1"
                     /translation="MARVLITGGTGFIGTHLTRRCIAQGHRVTVMALPGDPGIPNVAT
                     WPCDIVTGDIGDGALVETLCEGQEIVFHCAAVVSDWAPRALFNRVNVTGMENVCRAAL
                     RAGVRRLVEISTNDIFGLREDVVMDESFPLEPWGEPYPDTKIAATRIAWRYHREHGLP
                     VTMVFPCWVYGPGDKTFVPLTADAIVKGEMLFWRRDVLVWPTFVGNLVDLLILISERD
                     EAVGQGYLVHDGEATTFQEFCRGIAAALDVAPPKLHIPYGAAYAAAWLMEAAWRILGI
                     EVRPLLTTYSVKNLGSRLQFSIAKARRELGWEPAVSYAQGFAETMAWLKRQDLTSLKG
                     K"
BASE COUNT         3571 a         5854 c         6131 g         3311 t
ORIGIN      
        1 ctccggagat catcgccctg tacaagggaa agttcaagat cgacccctac atgcaggagc
       61 acatgccgag gacggtcgcc gtcctgccct tctatgacct gtccaaaagc gggaaggggt
      121 tcgacgcggt gcggaagggc ttttacaacc acttcagcgc cgttccctac caggacatgg
      181 aactctacaa ggtcgacgcc ctcctggcaa aggccggcct gaccgatccg gacatcctct
      241 acaagacccc gtacgaggaa ctgggaaaga tcctgggggt cgacgccatc gtcctgggcg
      301 agatctccga tttcgacaag ctcttcgcga tcctgtattc ccatgtcgcc gtgggtgcgg
      361 aagtccggat gtacgacgcg aagacggggc atttcctctg gagcggaaag cacacggtcc
      421 gcatccacga gggcggcgtc tcgaccacgc ccatcgggat catcgccacc attgtagcga
      481 cgtccttgaa catgagagac atccagctgc tccgggcctg cgacgacctt ttccgcgaca
      541 tggtcaaaac cattccggcg ccctccctgg ccgaggccct gagaccgccc acgatcacgc
      601 tgttgaccca ggatacgaag ggcctcccca agaaggccgg ggatgaagtc cggatcgtca
      661 tccaggggac gccgaagatg cgggcgcgtt tcgacatcgg cgactaccgg cggcgcatcg
      721 acatgcagga gatcgagccc ggcggctacc tgggggtcta caaggtcctg ccgggggaca
      781 atgtccagaa ggccactatc accgggtatc tggaggatga cgcccggaat atggcccagt
      841 gggtcgacgc cgtcggatcc gtcaccctcg atacgacccc gccggacatt ccgaagaatt
      901 acaaatccgt cggccgcaac ggtctcgtcc tgctgaactg ggagaagagc gtcgcgccgg
      961 acctggtcgg ataccggctg taccggagcg agacgcccct caccggcttt accgagatcg
     1021 cccggacgga attgaacgag caccgggatg cggggttgac gaacgatcgg aagtactatt
     1081 ttcagatttc ctcggtggat caggccggga acgaaagcga acggagcgaa gtgatcgtcg
     1141 gtatgcccgt cacaccgggc cccacgcccg tttcgggcgc catcgaggcc gatacgatct
     1201 ggtattcagg cgccagcccc tacgtgattg aaaattccgt catcgtccgg gacaaggccc
     1261 gcctcaccat agagccgggt acggaaatcc gctcgggggg gggggggatc acggtggagg
     1321 gaagattgct ggctcagggg gacggggagc atatcatcac cttcgatgcc gaggagggga
     1381 aaacctggga ggggattacc ttcctgaacg tcaaggaaaa ggaaaacgtc ctccggaacg
     1441 ttcgcatccg caaggcgcgc atcggggtgc tctgcatggc cgcctcaccg cggatcgagg
     1501 cctcggaatt cgtcgagaac gggacggccg tccggatctc gggcgccttc tcgaagccga
     1561 acctggttca gaataccctc cacaggcaga aagaggcggc ggtggtcgtc gaggccggcg
     1621 cccagccgct cctggcggaa aaccgcatcc gcgacaatga ggggaagggc attgtcgtcc
     1681 aggcgtcctc gcccgtcatc acccgcaacg tcattgcgca gaaccgcggc gtcgggatcg
     1741 acctgaagac gagccccgcc cgcatttcgg aaaacaacct gaccgacaat cggccttttg
     1801 acatgacggc ggaaatgacc ggagaggcgg tgaacgcgtc cggtaactgg tggggcaccg
     1861 cccaggggct cgaggtgctg aaaaagatcc ggggccgggt cgatgtcgcc accgtcctga
     1921 gcgcggccta tcccgacggg aagccggtcg cgctgccgat cctttcgcag acgctggacg
     1981 gggcgatcac ggccgacggt ttcctcacgg tgtcgaacag cccctaccgg gtgcagaagg
     2041 atgtcaccgt cgacggcggg gctacccttt acgtcgagcc cggcgtggag atccgcttcg
     2101 atcagaacac ctccctgatc atggcggacg ggggcatcgt ggccaaggga accccggatc
     2161 ggccgatcgt cttcacggcc tcggggacaa cgcccgcgcc gggattctat acgaatgccg
     2221 ttcgattcac gaagaagacg aaggtgaaca gcgccctggc ctactgcgag gtgaaattcg
     2281 cttccacggc ccttgatgtc tacaccggcg ccccggaaat ctcccactgc ctgattgccc
     2341 agacgtctca gggcggcatc ttctgccgca acgacgcagc cccccggatt tcctacagca
     2401 ccttcaccgc gaacgccggc gaaggcgcga tcaagagcgt cggcatgtcc aatccgtcga
     2461 ttcatcggaa caattttatc gacaatcccg tcgccctgca gatcttttcc tcgatctata
     2521 tcgatgcgcg cgaaaactgg tggggaaagg cgcccccgga cgtcagtctg atctggggcg
     2581 atctggacag gaatatcaac atcaagccct ggttggaaaa accggaggac agggccttcc
     2641 ggggcagata agcctcgggg ggcaatttcc ccgttgaaag gggggggaga ttctgttatc
     2701 gttccgaaca tgcggcaaca gggggtcgcg tttgccgagg ggatgtttct atgggtgatt
     2761 ccataaccgt caaggaggat gtcatgaagt caaaatgggt actgacgata gggattttgc
     2821 tggcgggcct gtttctgtat gccggtgtgg gcctggccgc cgatacggtg agcatgccgg
     2881 aatggacgca gattatcgaa cagatggccg gcaagggcaa gatcaactgg tcggaggggt
     2941 acatcgaggc cgtgggcatc ggcgccccgc cggaaaggtt cattgggaag ccccaggccc
     3001 gtccactggc gctccgggcg gcccgggtcg acgcctaccg caaccttctc gaactcacga
     3061 agggggtccg ggtggattcc atgacggtgg tcaaggactt cgccgtggag agcgacgtga
     3121 tcaataccca ggtggagggt gtggttaagg acgcccacct cgttaaacag gactacctct
     3181 cggacgggac cgtggaaata acgctccgga tgccgctggc aggcggtttt tcccaggtga
     3241 tcattccccg gctgatgcag aagaggtccg taccgccgga accggcggcg cccttagccc
     3301 ctcccgcggc ggcgccgcct gagcccgccg cgccggcccc tgcggctcct ccggaggttt
     3361 tcaccggact cgtaatcgat gcgcgcggtc tgcaggcaag acccgccatg tcgcccaaga
     3421 tcctggatga aagcgggaaa gaggtgtacg gctccctgaa cgtggatcgc gaatacgcta
     3481 ttcagcaggg gatgagcggt tacgcccgcg atctcaacgc cgcccagagc aacgcgcggg
     3541 tcaccaacaa tcccgtcagc ctcaagggat tgcggaccga aggaccgggc cgttccgaca
     3601 tcgtgatcag caatgccgat gcgaataaaa tccgcggcgc cgccgaaaac ctgagtttcc
     3661 tgaaacaatg ccgggtcatg atcgttctgg actgaccctt tccgggacgc gttcataggc
     3721 aggatttcga ccgcagtccc caccgggact gcggtttttc ttgtcggttc gctcgggggc
     3781 gcaccccggc gggaggatga tctgctgtgg ggaaaatcac cgttctgccc gaggaactga
     3841 ccaaccgcat cgccgccggc gaggtcgtgg aacgtcccgc gtcgatcgtc aaggaactcc
     3901 tcgagaatgc ccttgacgcc ggggccgaaa ctgtcggcat cgagttgcgg cagggggggt
     3961 gcggcgccat tcgcgtcacc gatgacggcg gggggatggc ggccgccgac gttcccctgg
     4021 cgttttcccg ttttgccacc agcaagatcg cctcttttga agacatttat cgaatccgtt
     4081 ctttcggttt tcggggcgag gcactgccca gcatcgcggc cgtttcgcgc gtcaccctga
     4141 ttacgcgccg tcgcgaccgg ccggacggaa cgcgcctgat cgtcgaggcc ggccagatta
     4201 tcgaggtgac cgattgcggc tgtccttccg gcacctccat cctcgtcgaa aacatcttcg
     4261 accccgttcc cgtccggaag aaattcctca agcgtgaatc cacggaactt tcggcttgcc
     4321 tggaggcgat cacccgggtg gcgcttcccc accgccatgt ccgggtgcgc gtccaggcca
     4381 acgggcgcag cgtgatgagc cttcccgcga cggaaggaga cctggaacgg ctggggcttc
     4441 tgttggggga ggaggcggcc ggccacctac tgcctttttc gggcttccgg gaaacagcct
     4501 ctctctcggg attttgctcg cgtcccgcct ggacccgctc cgacggtaaa cagatcttct
     4561 gctacgtcaa cggccgcttc gttcgggacc ccctcctgca gcacgccgtc atgacggcct
     4621 atcgccgcct catcgaagcc aggcgctacc cggcctgcat tctttatctc gacgtggctc
     4681 ccgaagaggt ggatgtcaac gtccatccag ccaaggcgga ggtccggttc cggcaacccc
     4741 gggacatcta cgcgctgatc gtcgagagcc tggtcgcaat cctttcggga tcgaccgccg
     4801 ttccgtccgc gcctctggcc gacatcgcgc gggagcccct gtatcaggcc cgcatcgagg
     4861 aggccatgaa gcggtacacg atttcctccg ggaggagcaa gcgcctcttt gcggggctgc
     4921 cggcggcccc ggcggttcgt ccggaccgcg acgcggggtt cccgacgccg cccacgacgg
     4981 cggctccgtt cccggaggcg gcgatccgct ttaccgaggg gacctacctg ggacaggtcc
     5041 tgtccaccta cctagtcttt gcgcatcccg ccggcattct cctgatggat cagcacgcgg
     5101 cccacgagcg ggttcttttc gaacgcctgc gaacgagcgg gacggcggga aaggtcgtga
     5161 cccagcagct tctcctgccg gaagtgatct cgctgcgcgc cggcgagttc caactcgccc
     5221 tgtcgctgca ggcgctctgg gacgaactgg gcttcgccat cgagccgttc ggggacacca
     5281 gcatcgccgt caaggccgtt cccgccctcg tcccgaacct cgatccccgg cagacggtca
     5341 gagaccttct gtccgaactg tccgaactgc ccgcgacggc cgggctccgg gaacagcggg
     5401 accggctcca cgcggtcctc gcctgcaagg gggccgtcaa ggcgcatcag gagctgacgg
     5461 aggcggaggt cggcgccctc tgccgggaac tggaagccat ttccttctcc tccacgtgtc
     5521 cgcacggacg ccccgcgtat gtgctcatcg acgaacggga cctgaaacgg atgttcagga
     5581 gaacctgagg cgaagggtcg gctgaggagg aaatgacgtc cggagacagg ccgaaactca
     5641 ttgtgatcct ggggccgacg gcggtcggga agtcgtcgct ggcgctcgag ctcgcccagg
     5701 cgtacggcgg ggaaatcatc agtgccgact ccatgcaggt gtaccgggga atggatatcg
     5761 ggaccgccaa gccgggccgg gaagcccgtg cgcgcgtccc ccaccatctt atcgatgtcg
     5821 tcgatccgga cgaacccttc aacgccgccc tgtttgttga acaggccggg caggtcatcg
     5881 accgtctgca tcgcctgggt aagccggtgt tcgtcgtggg cgggacgggg ctctatatcc
     5941 gcgcccttct aggggggctg gtcggcggcc ccggcccgga cgaggccctg cgggcgcaat
     6001 ggagggcggt ccgagacgag aagggcgttt cacatcttta tgaacagctc cgacggctgg
     6061 atccccgggc ggcggggcgg atccatcctc acgatgcggt ccgtatcgtg agggcgctag
     6121 aggttctcgc cctgaccgga gaatccatcg tcggcaaaca ggccgagcat cgctttgccg
     6181 aacggcgcta ccgcagcctc aagatcggcc ttcggctgga gcgggaaccg ctctttgccc
     6241 ggatcgaggc gcgcgtcgac gccatgattg ccgccggcct cgtggacgaa gtcgcgggac
     6301 tggcggcgcg cggctacgat gcgtcgctca agccgatgca atccctgggc tatcgccata
     6361 tcggaggctg gctccgcggg gaggctgatc tttccaatgc ggtccggagg ctgaagcgcg
     6421 atacgcggca ttacgccaaa cgtcaacaga cctggtttgc ccccgatccg gagattcgct
     6481 ggttttctcc cggcgatacg gcgggcgtgg aggcctgtct gcggccgttt ctgaaccgtg
     6541 aaattccttg acttaaaggg gaggggaaga ttataaaaac agccaaatcg gtggttgaag
     6601 gacaaaagga atccccgggc gggttccagg agaacggtct tttcaagaat aaggaggatg
     6661 atatggttcg tctattcaag aatcgaaatc ttaccacggt cctggttctc gtcgcggcgg
     6721 ccctgcttct cgtttcctgc ggtcccaagg ccagggtggc gaccagcgaa ctggacacgc
     6781 cggagcatca cttttatacg ggcatgaagc tgctggatca gggacgctat gtcgattccc
     6841 agcaatcctt caacttggcc atccagctgg accccaagta ttccaaggcc tacgcaggca
     6901 gcggccttgc caatgcctat cagcgggatt tcagcgccgc tttcgcgatg atgaagaaag
     6961 ccgggaaata tgcgaaaacc gaggaggaaa aggtcttcgt ccatgtcggc tacatccgca
     7021 tcaacaccct gagcgccgcc gactgcctcc ggtcgggcat ccgcaccggc gtggtgtgcg
     7081 acccgccgtc cgactggctg gatacggcca agggagaatt cgacgacgcc gtgctcatcg
     7141 atcccaagtc ctcggccgct tactatttta tgggaatcgc ctacaaaacg gccctggaca
     7201 tcaaccaggc cgcctccatg ttttccaagg tcctggatct gaagggcgac tacgtgcgcg
     7261 aggccgacga tcagtggcgc ctcgtccaga agatccagcg ggccatgccg ggcacgatca
     7321 ccggaaagaa gatcgccttc gtggagcaga tcacacgcgc cgatgcggcg gccctgttca
     7381 tggaggaatt gaagatcgat gtcctgtatc agaagcgcac cctgaaaacc ttcgacacct
     7441 cattcaagga tcccgagaag gccaaggaat ccggtccagc cccgaaaacg gccaacgaca
     7501 tcgccacgca tcccctgaag gcggacatcg aggggatcat ccagttgggc gttcgggggc
     7561 tcgaggtttt cccggatggc gcctttcacc cgaacgacct gatcgaccgg gcgagttacg
     7621 ccatgatgat cgaggatatc ctcatgaagg tgagcggcga cagcaccctg gcgaccaagt
     7681 tcatcggaga gacggcgtcg tctttccccg acttgagaac ggacctgccc tattacaacg
     7741 cggtgcgggt ggtaacgtcg cgcggcatca tggagccaaa ggatctggca tcgggcgaat
     7801 tcgggcccct gggtccggtg ggcggggccg actccctgct catcatccgt aaattcagcg
     7861 acatcctgaa gatttattag gcaggagaga aatcctatcg ggaaagatcc gaacaggcaa
     7921 gaccaagacg cgggggatgg caggggaaag agattctccc tgccgtcccc cgtggcatca
     7981 tagcggaaag atcccctcga tcgcccctcg aacagtgcgg agcgcagcgc ctccgcaggg
     8041 gaggaagggg gattttttca tggagtgtat caatgggcgg tggaacttta agtaaaacct
     8101 acgaaccccg ggattccgaa gcgaagtggt acgcctactg gctggagaag ggctttttcc
     8161 acgcggtcga tgacggcgcg aggccgccgt tcgccatcgt catccctcct cccaacgtaa
     8221 cgggcatgct ccacatgggc catgccctga acaatgtctt gcaggacatc atcgtccggt
     8281 tcaagcgcat gcagggctac aacgccctct ggatgcccgg catggatcat gccgggattg
     8341 cgacccagaa cgtcgtggag caggaactgg cgcgtgaggg gctcacccgc cacgatctgg
     8401 gacgggagaa gttcgtcgag cgcgtttggg agtggaaggc caaatacggc ggcgtgatca
     8461 tcaaccagtt gaagcgcctg ggctgttcct gcgactggga gcgcgagcgc ttcacgatgg
     8521 acgaggggct gtcccgcgcc gtccgggagg tgttcgtccg cctgtacaac gacggcctca
     8581 tctaccaggg agactacatc gtcaactggt gcccgcgctg ccacacggcc atctccgacc
     8641 tggaggtgga gtacaagcag gagcccggct ttctctggaa catccgttat ccctttgccg
     8701 acgggaacgg cgaaatcgtc gtcgccacga cccgtcccga gacgatgctc ggcgatacgg
     8761 cggtcgccgt caatccggaa gacgaccggt accgggccct cgtggggaag cgcgtcgtcc
     8821 tgccgctggt gaacaaggaa atacccatca tcgccgatga ctacgtgacg ctggacttcg
     8881 gttccggcgc cgtcaagatc acgcccgcct gcgatcccaa cgacttcgcc atggcggggc
     8941 ggcacgatct cgaaatcatc aagatcatgg acggcagcgg cgtgatcaac gcgaacggcg
     9001 gttcgtacga aggtcaggac cgctacgaat gccggcggaa cgtcgtccgg gatctggaag
     9061 agcagggcta tctcgtcggg acggagccct acgtccacaa catcggccaa tgctaccgct
     9121 gcaagacgga catcgaaccg atggtctcca agcagtggtt cgtcaagatc cagcccctgg
     9181 cccgggccgc cacggcggcg gtcgtcaagg gcgagacccg gatcgttccg tccatgtggg
     9241 agtccaccta ctacgaatgg atggacaaca tccgcgactg gtgcatctcc cggcagatct
     9301 ggtggggcca ccggatcccc gtgtggacct gcggcgggtg cggccggatg atcgtcgcca
     9361 cccaggatcc cgagacgtgt cccgattgcg agggccggga cctgcggcag gaggaggacg
     9421 tccttgacac ctggttcagc tccgccctct ggcccttctc caccctggga tggcccgacg
     9481 ggacggcggc cctgaagacc ttctacccca cgtcgctcct cgtcaccggg ttcgacatcc
     9541 tgttcttctg ggtcgcccgc atgatgatga tggggcttta cgtcatgaag gacgttccct
     9601 tccgggacgt ctatctccac gccctggtcc gagacgagaa gggcgacaag atgagcaagt
     9661 ccaaggggaa cagcatcgat cccttggaga tgatcgacaa gtacggaacg gacgccttcc
     9721 gcttcaccct ggcggccttc tcggcccagg ggcgcgacgt gcgcatgtcg gaggagcgga
     9781 tcgagggcta caagtatttc gtcaacaaga tctggaacgc gacgaagttt tcgctcatga
     9841 acctgaagag cgagccgaag gggaccgaaa cggttccgcc gcaggacctc tccctggccg
     9901 accgctggat ccgttcgcgc ctcaacagga cgaccgccga ggtcatccgg gggctcgacg
     9961 aatacaaatt caacgaagcc gccgccgccg tgtaccagtt cgtctggcac gagttgtgcg
    10021 actggtacct cgaactgatc aagccggtcc tctacgggag cgaggacaac gcggccgtcg
    10081 tcgccgcccg ccggacgctc cgccggacgc tccagacctc gctcaagctt ctgcatccct
    10141 tcatgccgtt tctcacggag gagatctggc agaccctcgt ggcggacggt acgtccatca
    10201 tggtcagcga gttcccgcga gtggacgaaa cgccggccga tccggaagcg gaggcgcgca
    10261 tggggctcgt catggacgtg atcagcgcga tccggaacat ccggggggag gtcaacatcg
    10321 ccccttccaa gaaggtgaac gtccggatct ttgcgccgga ggccgagggc gcggccgtcg
    10381 tcgctgccga gcgcggccat atcgtcaacc tggccaacct ccagagcctc gagatcgacc
    10441 ggaccctcga agagcccaag ggggccgcca ccggggtcgt cggcggcctg cgcatcttcg
    10501 tccttctgga aggcgtggtc gatatcggcg cggagcgggc ccggctacag aaggaactgg
    10561 ccaaggtgaa ccgcgatctc gaaggggtgg cgagaaagct cgccaaccgg gattttctcg
    10621 cccgggcggc ggccgccgtc gttcagaagg aagagcggaa acaccgggat ttgacggaca
    10681 aacaccaggc tctggaggcg gccctgaagc ggtttgccga tctggggtga gcggacggtc
    10741 cccatgctgc cgaaaacggt gctcgacccc attctgagac aggccctggc cgaagacatc
    10801 ggccccggag acgtgaccac ggaggcgatc ctgaagggcg acgagcgggg acgggcctgc
    10861 gcgatcgcca agtcggacct ggtggtggcg ggcggggagg tgttccggga ggtcttcacc
    10921 ctgatggacg cctcgctgga agtcgtcctg tcccgccggg acggtgaccg ggcccggccc
    10981 ggggacagcc tcgcggaact gaacggccgt ctggacagca tcctgacggc cgagcgcacc
    11041 gccctgaatt tcttccagcg cctctgcggc atcgccacgc ttacgcggcg cttcgtggag
    11101 gaaatcgatg gaaccggggc ccgaatcctg gacacgcgca agacagcgcc gggcttgagg
    11161 cttctcgaca agtacgccgt acgggtcggc ggcggacgaa atcaccggtt tgcccttttc
    11221 gacggtgtgc tcatcaagga caatcatatc gcggcagcgg gcgggatcgc cgccgccgtt
    11281 gcgaaggtcc gtcagaccgt cgcgcatctc gtgaagatcg aagttgaatg caaaaatgcc
    11341 gtcgaggtcg gggaggcgct ggccgccggc gccgacgccg tgatgctgga caacatggat
    11401 ctgccggcga tgagggaagc ggtggccctg gtccgggggc gcgtcccgct cgaggcctcg
    11461 ggcaatgtca ccttggccaa tgtccggagc gtggccgaaa cgggggtcga tttcatctcc
    11521 gtcggcgccc tgacccactc cgtggcggcg gcggatattt cgctcggcat tcagcagggg
    11581 acatgagtcc gtcttttagc gccgcaagcc tgtcggagcg tctcgcgggc gtcggtctcg
    11641 cccggcacat ccttttttat gcggagctcg gctccacgaa tgaagaggcc ttcgacctgg
    11701 cccggcaggg cgcgccggaa ggaaccgtgg tcgtggcgga ccgccagact caagggcggg
    11761 ggcggatgca acgcgtctgg gtttcgccgc cgggacggaa tctttacctg tcgctcgtct
    11821 taagaccgga tatcgcgccg gagcaggccc ctcagttgtc gattgtggcc ggtgtcgccg
    11881 cggccgaggc gctcgtgggc ctgtgtcccg gtgtgacgct gaagtggccg aacgacgtcc
    11941 tcgccggggg gcgaaagctc tgcgggatcc tggccgaaat gaaggcctcc gccgggcggc
    12001 tggacttcgt gatcctcggg atcggcgtca acgtgaatct cctgcgggag gagtgcccgc
    12061 cggaaatcgc aggaatcgcc acctctctcc gggacgagac gggagggccg ctctcccgcg
    12121 tcgaggtggc cgccgtgttc ctccgctccc tggaggccgc ctaccggacc tattgccggg
    12181 aggggttccc gcccctccgg acgcgctggc tcgagcggac ggacatggcg ggaaagcccg
    12241 ttcgcgtcat ggcggggacg gacgtgtacg aaggccggat ggccgggctc gacgacgcgg
    12301 ggggcctgct gctcgaggag aagacggggg gcatccgagc ggtgatggcg ggcgatgcct
    12361 cggttgtgaa aggataagcg tatgctgctg gtggtggatg tcggaaatac caatacggtc
    12421 ctggggctct tcgacgggga caaactgatt cacgactggc ggatccgaac ggaggtggat
    12481 cacaccgtcg atgaatacgg gatgctgatc tacaacctct acaagagcgg ccgggtcggg
    12541 cagcgggaga tctgcgcgat catcatttcc tgcgtcgttc cccccatgct gaacatcctg
    12601 gaaccgctgt gcctgaagta cttcaacctg aagccgatca tcgtcgggcc tgggatcaag
    12661 acggggatgc cgatttatta cgacaatccg aaggaggtcg gggccgaccg gatcgtgaac
    12721 gccgtggcgg ccttcgagaa atacaaacgg gaactgatca tcgtcgattt cgggacggcg
    12781 acgaccttcg atcacgtcac ggtcaagggc gagtacatgg ggggctgcat cgccccgggg
    12841 atcatgatct cgagtgaggc cctgttcctg cgcgccgcga agcttccccg cgtggaactc
    12901 tccaggccga aggccatcat cgccaaggat acggtcagca gcatgcaggc ggggatcatg
    12961 ttcggctatg ccgggctcgt ggacggcatc gtcgggcgca tgaaagccga ggccaagtcc
    13021 aagccctatg tggtggccac gggcgggctt gccaagatcg tcgccgcgga aacgaagacc
    13081 atcgacaccg tcgacgaaat gttgaccctg gagggcttga ggatcatcta cctccgcaac
    13141 gccgctccct gaaccggttc cgcgccacga ccggtcgaat accctcctca tgacaacagg
    13201 ataggacgga atgctgaatg tcggattgac gggcgggatc gcctgcggaa agaccaccgt
    13261 ggcggagatg ctggtggcac ggggggcgca cctcatcgat tttgatgcgc tggcccatga
    13321 ggtcgaggcg ccggaccggc cggcgtggcg ggggatcgtg tccgccttcg gcgaaggcgt
    13381 tctcaacgcg gaccggacga tcgaccgccc gaagctcggg gcgatcgtct tcgaggacaa
    13441 ggccaaactt gagacgctga acgggatcgt ccatccggcg gtcttcgacg aatggcggca
    13501 gcggatggac gcgatcatgc acaacgaccc gcggtccatc atcctctcgg acgttccctt
    13561 gctcttcgag gtggggatgg cgcccctctt cgacctggtc gcgctcgttt atgtctcccc
    13621 cgcccagcag gtcgagcgcc tgatgaaacg gaacggcttt accgccgaag aggcccgggc
    13681 gaggctggcg gcccagatgt cgatcgacga gaaggtccgg ctggccgacg tcgtgatcga
    13741 caaccaggga accgtggagg agacgcggcg ggggatcgcg gcggtctggg cggatttcgt
    13801 gcgccgcgag gaggcgaagc gcatgtcctg agccgcggcg gaccgcacgc gccgacgccg
    13861 gggaagcccc ggtcgcgccc gcggcccgaa tgcggcggat acactcatat caagaaagag
    13921 gaggttccag cgtgatccac aagaatgtca aaaccgattt ttcgccggcg gccttgacgc
    13981 cgcagatcga tccggcgacg tacgtccacc ccctggccgc cgtcatcggg aatgtcatcc
    14041 tggggaaggg catcatggtg tccccctttg cctcgatccg cggcgacgaa gggcagccgc
    14101 tccatgtggg ggacgagtcg aacgtccagg acggcgtcgt gatccacgcc ctggagacgg
    14161 agttgaacgg tgcgcccgtg gagaagaacc tgcgggaggt ggacggcggg aaatacgccg
    14221 tctacgtcgg gcggcgggta tccctcgcgc accaggtgca ggtccacggt cccgcggtga
    14281 tcctggacga cacgttcgtc gggatgaaat cgctcgtttt caagtccgtg gtcggtaagc
    14341 agtgcgtggt cgagcccggc tgcatcctga tgggggtcac ggtcgcggaa aagcgctacg
    14401 ttcccgccgg atcggtcgtg aagacccagg cgcaggcgga tcagctcccc gcgattacgg
    14461 acgattaccc gatgaaggac atgaacaagg gcgttgttca cgtcaatacg gccctggcgc
    14521 gcggctatct cgccgccagc gagtagaaga atcgatcggg gcgccggcct gagcgccggc
    14581 gccccgcgag acgggcgaga ggggataccg gctcttattt gaagatcgag aagacggcct
    14641 tgcggacctt ctcgatgctg tcccgatccc tcaggatcgc ttccagttcc ccgggatcga
    14701 ggtggacggc gccgcagtct tcattgatgc acacgtcgat cttgaccgac ccgtggctga
    14761 tttccgccaa gctcggcgta ccgcagcgga agcagtgatt cttgtgctcc tcacggtact
    14821 tctggtccga ttcccgggcc gccttttcgc gcagcttccg gatcttctcc tgttcctgtc
    14881 ccttgaagaa caagtcttcg tgatctcccg tactgattgt gatgaacgtc ttttcctgag
    14941 ccatggcatc ctccttgatg aaacggcggc gcttgcggtc cgctctatcc gtgacgcgca
    15001 cttagcataa ttcatccacg gattcaacct ctccgatcgc cgggcgcgtt tcacgattgc
    15061 cgccggccgg ccctccccgg attgcacgcg gatgtccctg gcgggagcgt tcatcaaggc
    15121 gtcaaatcct tcacggatcc gtgcgcccga tccaccgata gacggtggcg ccgagaattg
    15181 cgcccacgat gggggccacc cagaacagcc acaactgcgc taccgcccac ccgccgacgt
    15241 agagggcgac gccggtgctg cgcgccgggt tcacggaggt gttcgtgacc gggatgctga
    15301 tcagatggat cagggtcagg cacaggccga tcgcgatcgg ggccagaccc tgcggcgcgc
    15361 gcttgtccgt cgcgccgagg atcacgatca gaaacatcat cgtcatcacg acctcggtga
    15421 ccagggcggc gagcagggaa tagccgccgg gggaatgctc cccgtagccg ttggacgcga
    15481 agcccgcggc cacgtcgaac cccgccttgc cggtggcgat caggtagagg acgcctccgg
    15541 cgacgatgcc tcccaggacc tgggcgatga tgtagggcac gagccccttc gccgggaagc
    15601 ggccgccggc ccacagaccg atggacacgg ccgggttgag gtggcagccg gagatgtgtc
    15661 cgatcgcgaa ggccatggtc aggacggtca aaccgaaggc cagcgcgacg cccagcaggc
    15721 cgatgcccac gtccgggaac gtcgcggcca ggacggcgct gccgcacccg cccagtacga
    15781 gccagaaggt tccaaaaaac tccgcaccga atgctttcat gtcttttccc tcctccgtct
    15841 tggggtgagt ccgacgtctt gcggacgatt ccgttcgacc ctcatccgcc cggtagccgc
    15901 cgccgggcgg aacccggcga cccgctgctc ataaaccctc gtagcggagc gtgttcttcg
    15961 tgaacacgtt ctcctcgtag atcagggcat gcgcatcgaa aaagcttttc cacgtgttct
    16021 tctcggttat cgaatcgcca gcctacttga ctttccgcaa ggcccggttc atggcctcga
    16081 tgtcgaacgc ctttgggtcg aattcccctc ccacccactc cagataggaa tcgtgttcct
    16141 cgtggttggg gtcgcggatg gcttgcagaa atctttgata gccccagata cccccgacat
    16201 cttccggcgg acaagcgcct ttcccctgta tgcatttcgg atagacttca cccgcatcag
    16261 gcgggatgat cttttccacc aggatgacgt gctcccaacc gtcgccgaaa tcatattcgt
    16321 agatgaacct ggagttcaag gagggggcga gtttttccaa cttgacgcgg cgctcatcga
    16381 tcccaggctc ccagtcgtcc gggtggggaa tgttgtagta cctgccatcc acaatgaatt
    16441 ggtgcaagtg gccatcttcc catcccatcg cgagttgaat cacccgatga aatttataca
    16501 ggctgaaatg gtcgggcacc cacacccgcc gccagatggg gggacgggac cctttgagcg
    16561 tgatcttgaa ttgatagacg gcagcgggaa tctttggcga ctttggcggc atggcgggac
    16621 tcctgggttt gatttgcggc ctacacgggg aatcaatcga cagcccgtta aacgaacgga
    16681 cctcgtacag ttgtgtgtca acggggatat catatgtgct cgcgtccaaa attcaaagaa
    16741 tttttttgga ccctggacca tcgcgtcacc gcccgttcgt aaatgatcgg gtggtcatgc
    16801 catcacacca tgcgactgtc gtgccttgaa tttccggttg aaaaaaccct tgacatgaga
    16861 tagggtgtcc gtcaagtaga aaccatatag tgttctctaa tacaacagcg gatggcgccg
    16921 gccggagaac gcagcaacga tcccggttgt cttgaccgat ggaaaaaggg ggggggcgat
    16981 gaagacggga gcggggcagg gacggtacgc ggaccaaaac gtgttcatca ccggcggctc
    17041 cagcgggatc ggcctggcca cggccgaggc ctttgccggt gagggcgccc acgtcgcgat
    17101 ttttgcacgc aacccggggc ggatcgagga ggccgtggcg cgaatccgcc gggcccggcg
    17161 ccggggagat gaaagggtcg cgggctttcc cctcgacgtg gccgacccca cacgggtccg
    17221 ggaggtcatg acgcaggcca tccgggaatt cggcgccccg cacgtcctgg tcaacgacgc
    17281 cggcgtcacc tacacggatt acttcgaaaa cattacctac gaacagttcg acgagatcct
    17341 ccacaccaat ctctacggga tgtggaacac catcacgacc ctgcttcccg ccatgaaggc
    17401 gcacggcggc ctgatcgtca acgtctcctc gatcgtgggg ttcctcggcg ttttcggcta
    17461 tgccgcctac gcggcgtcga agttcgggat cgtcgggctc tccgagacgc tgcgcatgga
    17521 actgaagcgc tacggcatcc gggtttgcgt gctgtgcccc ccggacaccg acacgcccca
    17581 gtggcacggg gagaaccgca cgaagcccag ggagacctgg gccctggcgg ggaacgccaa
    17641 ggtgatgaag cccgaggccg tggcgcaggt gctgctggcc ggcatcggga aggggcgatt
    17701 catgatcatc cccggcacgg aaggaaaatt catctacatc atgaaacggc atttccccgc
    17761 cctcgttgcc tggatcatgg accgggacgt ccgaaaggcc gcggcaaagg gagtctgaaa
    17821 ggcacggaaa ggatggggac atggcgaggg ttctgatcac cggcggaacg gggttcatcg
    17881 gtacgcatct gacgcggcgc tgcatcgccc aggggcatcg ggtgaccgtc atggcgctgc
    17941 ccggcgatcc ggggattccc aacgtggcga cctggccgtg cgacatcgtg accggggata
    18001 tcggcgacgg cgccctggtg gagacgctgt gcgagggcca ggagatcgtc tttcactgcg
    18061 ccgccgtcgt gagcgactgg gcgccccgtg cgctcttcaa ccgggtcaac gtcacgggga
    18121 tggagaacgt ctgccgcgcg gccctgaggg ccggcgtccg ccgtctggtc gagatcagca
    18181 ccaacgacat cttcggcctg cgcgaggacg tcgtgatgga cgaaagcttc ccgctcgaac
    18241 cctggggcga gccctacccg gatacgaaga tcgcggccac ccggatcgcc tggcgctatc
    18301 accgggagca cggcctgccg gtgacgatgg tattcccctg ctgggtctac ggtcccgggg
    18361 acaagacgtt cgtgccgctg acggccgacg ccatcgtcaa gggcgagatg ctcttctggc
    18421 ggcgggacgt gctcgtctgg ccgacgtttg tcggcaatct ggtcgatcta ctgatcctca
    18481 tctccgagcg ggatgaggcc gtggggcagg gctacctggt ccatgacggc gaggcgacga
    18541 ccttccagga attctgccgg ggcatcgccg cggcccttga cgtggcgccg cccaagctgc
    18601 acatccccta cggagcggcc tatgcggccg cctggctgat ggaggcggcc tggcgcatcc
    18661 tggggatcga ggtgcgcccg ttgctcacga cctactcggt gaagaacctc ggttcgcggc
    18721 tgcaattttc gatcgcgaag gcccggcgtg aactcggctg ggaaccggcg gtgtcctacg
    18781 cgcaagggtt cgccgagacg atggcgtggc tcaagcggca ggacctgacg tccctgaagg
    18841 ggaaatgacc ccctccagca gcacctg
//
LOCUS       DAOOLJ010000032        18379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3118_length_18379_cov_20.043659, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000032 DAOOLJ010000000
VERSION     DAOOLJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 18379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18379
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3118_length_18379_cov_20.043659"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1060
                     /locus_tag="PLW16_06620"
     CDS             110..1060
                     /locus_tag="PLW16_06620"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPB70614.1"
                     /translation="MGEGLSDYTKFCVDLRRHMWMAGVETELRRLIWQIAVTGKYISA
                     KIHESNRKLAGFRNMYDEDQLALDRSSDEILKNQLQFSGFVREYASEEQDAVVSIGHG
                     NEKYFVTADPLDGSSLVDTNLSIGTIIGIHRESMFCAGKQSMVAALYITYGPLITMVY
                     SAGKGTHEFVLNREGEYVLSEENIRLKEKGSIYSLGGLRKDWSPEHLSFVESLEAEGY
                     KLRYSGGFVPDINQILIKRGGVFAYPGLRNSPKGKLRLLFELQPMAFIMEQAGGAATD
                     GATDILAIPVQDLEQRSPVYIGSRFEVERAKEYLNAKNGR"
     gene            1150..2544
                     /locus_tag="PLW16_06625"
     CDS             1150..2544
                     /locus_tag="PLW16_06625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPB70615.1"
                     /translation="MRYDRVDALKEGVRDSLAIDAGEVRVLDEQRLRDAEIVRLVHTA
                     VFGTDTVVRQAARWLIRRAGAALGIFPSSIQPLYEAMGREAVSGFTVPAINIRALTFD
                     VAQAVLRATLAGQVGPVLFEIARSEIDYTRQRPEEYTAAVTAAAIRTGYRGPLFLQGD
                     HFQVNAKKYAQDPAAEVRAVKDLIWEAIEGGFYNIDIDTSTLVDLSQATPARQQATNY
                     TLAAELTTVIRDLEPAGITISVGGEIGEVGGKNSTVEELRAFMDGYRAVLREAGTDLA
                     GISKISVQTGTAHGGVPLADGSVAKVKIDFDTLAELSRAARREYGLAGAVQHGASTLP
                     DEAFDRFPAVGTAEIHLATGFQNILYDSRHFPGALRDRIYGYLKAELASERKETDTDA
                     QFFYKTRKKGFGPFKKDLWDLPEAVRQALGEELERQFAFLFGKLRVTDTRALLDRTLR
                     PVDVPTGVPAAWRA"
     gene            2732..3964
                     /gene="rpsA"
                     /locus_tag="PLW16_06630"
     CDS             2732..3964
                     /gene="rpsA"
                     /locus_tag="PLW16_06630"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPB70616.1"
                     /translation="MDEEKQAQVNTEPEEEKSFAELLAETSPDRGRLRPGERVEVTIV
                     KITSEWIFLDLGGKSEGYLDRSELLDAEGNLTVQEGDTIRAYFLSAKRNEKLFTTKVS
                     SGEAARSYIEDAWKSGIPVEGVVEKEVKGGYEVRIGGSLRAFCPHSQMGMPRQPQTES
                     PVGKTRTFRVVEYGSRGRNIIVSRRSILEEEDRQRKEALKDTLTEGMTIRGTVVSLQK
                     FGAFVDIGGIQGLLPISEIGWGHVEKVQDVLSVGQEIDAVLLRLDWANDRITLSLKST
                     LPDPWETVENTFPEGSVHTGVIARLTKFGAFVTLSPGIDGLIHISKIGRGKRINHPNE
                     VLTQGQTIEVRVEKVETAQKRISLTPAADAAAPGGEGDRNGDYQQYVPKSKESFGSFG
                     DMMKGKLPERSLGKKETD"
     regulatory      4023..4118
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4185..5366
                     /gene="metK"
                     /locus_tag="PLW16_06635"
     CDS             4185..5366
                     /gene="metK"
                     /locus_tag="PLW16_06635"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPB70617.1"
                     /translation="MQISSESVKVGHPDVVADVISAQVIGRILDQEKALGLTVATMPH
                     CGLEVFLGKNLCLIGGEVRARVKIDVEACVREAVLSLGYNDEAVGLDGRTMRVINEII
                     PQSPDINQGTALELNKDKEIGAGDQGIMYGFACNETPELLPLPYVLSSRMMRTFETCG
                     DPIFAPDGKGQVSVEYNTEVCGPRRLAKVLMSNAVDYRQINGLSREAIKARAKEIAFG
                     CLADWVDKDTVFLFNPTGEWQAVNSCSAADSGVTGRKLVVQFYGGYPGAQLGGGAVVN
                     KSPEKVDCSAAFGARYVAKNIVAAGIAEKCAVQLSYAIGIARPFSIYINTFSTCGSAT
                     DERLAQVVEELFDLRPQGLIERFDLLNGDLYRRIPKTLFMDDYPWEKTDMADKLMKAL
                     A"
     gene            5630..6388
                     /locus_tag="PLW16_06640"
     CDS             5630..6388
                     /locus_tag="PLW16_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPB70618.1"
                     /translation="MDEDLIKAYRLFLKDSIRKVIDFSRTDQNRGVAPPPVQKPCPPD
                     AERIDLTPCDRFGALGRIDLETAIRNRESRRSYRREPLAREELSFLLWATQGVRQPPF
                     AGHALRTVPSAGCRHAFETYLCVLNVRGLAPGIYRYLPMEHRLLLEFSEERLDEKIRR
                     AALGQHHPGSAAVTFIWTALPYRMEWRYGLAAHKVIALDAGHVCQNLYLACEAIGAGA
                     CAIAAYDQEAMDALLKIDGQDEFTIYLASVGKKL"
     gene            6475..8595
                     /gene="aspS"
                     /locus_tag="PLW16_06645"
     CDS             6475..8595
                     /gene="aspS"
                     /locus_tag="PLW16_06645"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPB70619.1"
                     /translation="MLFSERVFCGQLTLGEVGRRVLLAGWVDAFRDHGGLLFIHLRDR
                     SGIVQIVFSPEIAAADVCRRAAGLRAEYCIAVRGEVKQRREGTENPHIETGSMEVLVT
                     ELEVLSESDALPFAVSDKAMVAGAPSAGTDTVAEDLRLQYRYLDIRRTSMRDNLIARH
                     RIFQCVRDFLDSRGFVEIETPVLTMSTPEGARDYLVPSRVHPENFYALPQSPQLFKQL
                     LMIGGMERYFQLARCFRDEDLRPNRQPEFTQLDIEASFIDEEFLYELIEELTVRMFAV
                     GGITLPRPFARMTHAEAMDTTGSDRPDLRFGLRFVDVTSVFAQTRYSIFRQILERGGC
                     IKGINIQGQSGQLSKNVLQNEYAKEIVPSFGAKGMTWMRVEDGKFESNIVQFFSELEL
                     EALRGKFSAADGDVLILIADPSHATVTSALGQLRLHLANRLGLIPSNSFCPVWITEFP
                     LFEATDEGGVTSSHHPFTAPDRADFDPKNIEELLSLRSRAYDLVINGEELGGGSIRIN
                     DRDLQRRIFAALGLTEAETKEKFGFFLRAFDFGAPPHGGLALGMDRAVSMILKTPSIR
                     EVIAFPKNRGAACPLTGAPSPVKREQLAELGLLNLGSQDVLPGTAENENRIDRLSWVS
                     RIGLSADERPGMEDVLAQADALALLAAQNAGEGEALYTVAPVANRTRPGIQEKCSPLA
                     ETGQLLSRAPAVKGRFFKVASILE"
     gene            8610..9809
                     /locus_tag="PLW16_06650"
     CDS             8610..9809
                     /locus_tag="PLW16_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPB70620.1"
                     /translation="MDSIFFYRPPEAAVPADGPLKGMNIAIQPNVSAAGWPTDAGSNA
                     LARFVALEDATVVERLRQAGASICGSTRMSEFGFGLDGSRAGEAIKRNAAHAELVLDL
                     MGESRLAASAGDVCGFKPSYGLVSRFGLIGLIPSMECYGILSDSLKNIREILKAIAGP
                     DERDFSLPDETTPDFSPQNIDTGKTTVGIIKEAPGSLPARQESLYRAAIETLGDAGLT
                     VKELSLPDFPLFLPVHKIVGAVEASSCTGRYDSVRYGVRVPGAKNWNEMYLSSRGAAF
                     GPLLKSYLFQGTFFQFERYDAYEDACRIRARLVAGMRRLAAQADFWAIPVERGGPDAP
                     ASLADTYAQFALTAYANVAGLPALYLPPAQGRPHPGVQLSGPRLSDARLLALGEHLLN
                     ARRGGNA"
     gene            9806..11245
                     /gene="gatB"
                     /locus_tag="PLW16_06655"
     CDS             9806..11245
                     /gene="gatB"
                     /locus_tag="PLW16_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB70621.1"
                     /translation="MMEFEAVIGLEIHVELNCATKMFCDCSNRPGDKPNMNICPTCLW
                     FPGALPRLSREALEKAALLCLGLGAELQPRSAFDQKVYYYPDLPKGYQLSQAHLPLAR
                     GGAIDISDENGRPRKLRIHHIHMEEDVAKLVHEMEGRLPISLVDFNRAGAPLVEIVTE
                     PDFRTPSEAMEFLKALRTQVRYVGSSECSMENGTMRVDANISVRPKGTGQMNTKVEVK
                     NMNSIRHVGDAVAYEINRQSADVQAGKVVTLHTRLWDPDKKVTLAMRGKFEGPCIPDP
                     VVPVIELAPEWIERMRTRLPEMPAARAERFVSQHGLSGEEAVFLSADPEVAGYFEALI
                     GQGVSPRTAMHWLVTQLIPAVKDRGQELSGSAVPPARFAALLNMLARDEINAHAAREV
                     LGQLFDSDKAPEAIVSALGFRQVSDPDALDALIDKVLAEQPAAVADVRAGQGKALGFL
                     IGQVMQASGGKANPKVLRDLLAKKVSAAS"
     gene            complement(11270..13171)
                     /gene="metG"
                     /locus_tag="PLW16_06660"
     CDS             complement(11270..13171)
                     /gene="metG"
                     /locus_tag="PLW16_06660"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPB70622.1"
                     /translation="MEKVFYITTPIYYVNASPHIGHAYTTIVADVLARYHRAQGYRTF
                     FVTGTDEHGDKIAEAAQKAGVTPQTYADGISAQFRALWPELSVTNDYFIRTTDADHVR
                     TVQRILQKVYDAGDIYFGSYEGFYCVGCERFYMEKELVDGKCPDHQTAPEYRKESNYF
                     FRMSKYQDWLLGHIQAHPEFIRPERYRNEALAFLREPLEDLCISRPKSRLTWGITLPF
                     DADYVTYVWFDALINYVTALGYPDGARFDEFWPVAQHLIAKDILKPHGIYWPTMLKAA
                     GIEPYRNLNVHGYWNVDASKMSKSLGNVVKPLDLKDKYGLDPFRYFLLRDMVFGLDSN
                     FSEEAFVQRINADLANDLGNLVSRTLTMAAKFLSGQAPAVTEAEAEASPLPETARRIL
                     AEVEADFAELGLHKALMVIWEFINVANKYIVENEPWTLVKEPERRGRLEAVLYSVLEA
                     LRVIAVLIAPFMPSTAEKILSQLGIEGGTQDFDSIRSWGGLPAGSRLTRGEALFPRVE
                     FRKEAVPMSEPKEEMVPVKPEIAYEDFARVDLRVATILEAEAVPKSNKLVRLKIDLGE
                     ERQIVAGIAKDYPPETLVGKKIVVVANLKPTKLMGVESRGMLLATDTPEGLTLIGFDR
                     DPKTGAPVR"
     gene            complement(13228..14016)
                     /locus_tag="PLW16_06665"
     CDS             complement(13228..14016)
                     /locus_tag="PLW16_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HPB70623.1"
                     /translation="MRNIVGVKFKQEGKIYSFHAGDLELRKGDPVMIQTENGPALAWV
                     ATDVRQVPLPKVPATLKNILRKMTPEDQKIKADNDRLEGEAQAFCLVRIRERGLPMKL
                     VDVECLFDRSKVIFYFTAENRVDFRELVKDLVQKFRSRIELRQIAARQEARLIKGLGV
                     CGREICCATFLQNLDRVSVKMAKEQSMSLNPEKISGLCGRLMCCLAFEHETYLDMKRD
                     MPKCGKVIQTPEGRGRVTRQNVFEGQITVVLEGGKEITLNVTDL"
     gene            complement(14052..15026)
                     /gene="holB"
                     /locus_tag="PLW16_06670"
     CDS             complement(14052..15026)
                     /gene="holB"
                     /locus_tag="PLW16_06670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPB70624.1"
                     /translation="MPFRDIYGHKRPLGILKTVLERGRIPQALLFHGMEGIGKRTTAL
                     AFAQALNCREGGTDACGRCPSCRKIGHGTHPDVSTLTADGQYIRIRDIRALQEQMQFK
                     PYEGRWRVFLVREADRMNLEAANALLKTLEEPSAKHGLILLTAHPDRLPRTVLSRCQQ
                     VRFHPLPRETVQRFLVERHALEAPTAGLLASCSGGSIARALNLRQGDFLEMRRALLER
                     MASPALREPWQRLSFLGDLGQERRSLAERLAVMRSCWRDALVVRETGAADLLIHRDCE
                     AEIRTLADRRTGEEILNNIRLIDRAIAALEQNANKQLTLEVMMFRLAL"
     gene            complement(15013..15669)
                     /gene="tmk"
                     /locus_tag="PLW16_06675"
     CDS             complement(15013..15669)
                     /gene="tmk"
                     /locus_tag="PLW16_06675"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPB70625.1"
                     /translation="MQPLITFEGIEGCGKTTQMKMAREVLRNHHLPHLLTEEPGGSPL
                     GRRIRQILLQQSPEEICPEAEILLFCAARAQHVRETILPALRRGEWVLCDRFSDASLA
                     YQGFGRGLDLECIRRIDAFASPLRPALTFLFDLPVETGLQRALDRISRAGGEAPEDRF
                     ENEALAFHRRIREGYLTLARENPERFRIIDATRDIFTIHKDVSVQLAAFIKGQTHALP
                     "
     gene            complement(15767..15946)
                     /locus_tag="PLW16_06680"
     CDS             complement(15767..15946)
                     /locus_tag="PLW16_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB70626.1"
                     /translation="MAYIITDECIACGSCQDECPVEAISEGDDKYVIDASLCTDCGAC
                     ADQCPVEAIVPGDDK"
     gene            complement(16067..16291)
                     /locus_tag="PLW16_06685"
     CDS             complement(16067..16291)
                     /locus_tag="PLW16_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPB70627.1"
                     /translation="MKEKVTQALDKIRPNLQADGGDVELVEVTDDGVVKVKLTGACQG
                     CPMSQMTLKNGIQRLLLQEVPGVKEVVSVA"
     gene            complement(16389..16982)
                     /locus_tag="PLW16_06690"
     CDS             complement(16389..16982)
                     /locus_tag="PLW16_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70628.1"
                     /translation="MMRKKTGLMLIAALLVLSLAGCGGLRYSQVDPESKYFHPRRIGV
                     LPVDVGTYEEARGNVDQTLAGVLIDKKWFADVVSGDTIARQFSANEELRKVVMDYMAK
                     LKTVNFSDPQQSKRIGELTQVDALLMVNVDDWNYAVENGEKVAKVGMGMKLIDVTTGK
                     IMWKAGHVAIEKYRVFKPKLLDVARDLVKEMIEYMPH"
     gene            complement(17007..>18379)
                     /locus_tag="PLW16_06695"
     CDS             complement(17007..>18379)
                     /locus_tag="PLW16_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70629.1"
                     /translation="IIGDRSHPEVNGLLGYAGDRGTVISSAEDVSRLPTLDKVCVVAQ
                     TTQGVEEFETLVGDIRDRFPETVVFNTICDSTEKRQTEVRTMAAEMEAMLIVGGQNSA
                     NTQRLARLSERQGTPTFHIETAEDLGGVDLSRYRRIGVSAGASTPNWIIHRVVDYLEN
                     DRGEKRRGLFAFWTLTVRTEVFSAIGAAGLALACMFLQGLRPDVLNLLTAALYVYAMH
                     TLNRFLARKGSIIGSFREATYRRHERLFLSLAVLAMVLALTLACLSGWAPFILLFTLS
                     ALGLVYNLRILPSHWRFRGLCDLPGSKNVAMALAWAAVTAFLPRIAVGFEAPPSLMVA
                     FCLAFSLVFVRSALSDILDIQSDRLIGRETIPVLIGKDRTLVLLKGIALLTALVLVAA
                     PFAGWAPWRIWTLMSCPFYVWICFTICDREAWFPGVLLEGLLESVYLVAGLSAGVGLF
                     FPRAFL"
BASE COUNT         3444 a         5804 c         5849 g         3282 t
ORIGIN      
        1 cgtaaaatct gcttgtcaaa cgggccacaa cctgttagta aacagccgca tttttcctgt
       61 ggattcaggc cgccaaaggc ggtctgcaca gaaacgaagg aaacacggca tgggcgaggg
      121 cctttccgac tacacgaaat tctgcgtcga tctgagacgc cacatgtgga tggccggcgt
      181 ggaaacggag ctgaggcggc tgatctggca gatcgccgtc acgggcaagt acatctccgc
      241 caagattcac gaatccaacc ggaagctggc cggcttccgg aacatgtacg acgaggacca
      301 gctcgccctg gatcgcagct ccgacgagat cctgaaaaac cagctccagt tttccggctt
      361 cgtccgggag tacgcatcgg aggaacagga cgccgtcgtt tcgatcggcc acggaaacga
      421 gaagtacttc gtcacggcgg acccgctcga cggatcgtcg ctcgtcgaca caaacctgtc
      481 gatcggcacg atcatcggca tccaccggga gtcgatgttc tgcgccggca agcagtccat
      541 ggtcgccgcc ctctacatca cctacggacc gctcatcacg atggtctatt ccgccggcaa
      601 ggggacccat gagttcgtgc tcaaccggga gggggaatac gtcctgtcgg aggagaacat
      661 ccgcctgaag gagaaggggt cgatctacag cctgggcgga ttgagaaagg actggtctcc
      721 ggagcacctg agctttgtcg agtccctcga ggccgaaggg tacaagctgc ggtacagcgg
      781 gggcttcgtt ccggacatca accagatcct catcaagcgg gggggcgtct tcgcctatcc
      841 ggggctgcgg aacagcccga aggggaagct caggctcctg ttcgaactcc agcccatggc
      901 cttcatcatg gagcaggccg gcggcgccgc caccgacgga gcgacggaca ttctcgccat
      961 cccggtccag gatctggagc agcgctcgcc cgtctacatc ggcagccggt tcgaggtcga
     1021 acgggcaaag gaatatctga atgcaaaaaa cgggcgatag gcaacaggag aatggccgga
     1081 gatcaagggc agacggaccc gggatgtccg ggctttcgtc ggagcgtcgc ctgtcagagg
     1141 ttatcgttca tgaggtatga ccgtgtggac gccctgaagg agggtgtgcg ggacagtctg
     1201 gcgatcgacg cgggcgaggt ccgggttctg gacgagcaac gtctgcggga cgccgagatc
     1261 gtccgtctgg tccataccgc cgttttcgga accgacaccg tcgtccggca ggccgcccga
     1321 tggctgatcc gccgggccgg ggccgccctc gggatcttcc cgtcgtcgat ccagcccctg
     1381 tatgaggcca tggggcgcga agcggtttcg ggcttcaccg tgccggcgat caacatccgc
     1441 gcgcttacgt tcgatgtcgc ccaggcggtc ctgagggcca cgctcgcggg acaggtcgga
     1501 cccgttctct tcgagatcgc ccgctccgag atcgactaca cacggcagcg gccggaggag
     1561 tatacggccg ccgtcaccgc cgccgccatc cggacgggct atcgggggcc gctctttctc
     1621 cagggggacc acttccaggt caacgcgaaa aaatacgcgc aggatccggc cgcggaggtc
     1681 cgcgccgtca aggatctcat ctgggaggcc attgagggcg ggttctacaa catcgacatc
     1741 gacacctcga cgctcgtcga tctgtcccag gcgaccccgg cgcgacagca ggcgacgaac
     1801 tacacgctgg ccgcggaact gacgacggtg atccgggatc tcgaaccggc ggggatcacg
     1861 atctcggtgg gcggggaaat tggcgaggtg gggggaaaga acagcaccgt ggaagagctg
     1921 cgcgccttca tggacgggta tcgggcggtc ctgcgggaag ccgggacgga cctcgcgggc
     1981 atcagcaaga tcagcgtcca gaccggcacg gcgcacgggg gcgttcccct ggcggacggc
     2041 agtgtcgcca aggtcaagat cgacttcgac acgctggcgg aactctcccg ggcggcccgc
     2101 cgcgagtacg gcctggccgg ggcggttcag cacggcgcct ccaccctgcc ggacgaggcg
     2161 ttcgaccgct tcccggcggt gggaacggcg gagatccacc tggccacggg atttcagaac
     2221 atcctgtacg acagccggca ctttcccggc gcgctcaggg accgcatcta cggctatctg
     2281 aaggcggagc tggcctccga gcgcaaggaa acggatacgg acgcgcagtt tttctacaag
     2341 acgcgcaaga agggcttcgg tccgttcaag aaggatctct gggaccttcc ggaggccgtt
     2401 cgtcaggccc tcggggagga gctggagcgg cagttcgcct tcctgttcgg aaaattgcgg
     2461 gtgacggaca cccgggccct cctggaccgg accctccggc ccgtcgacgt tccaacgggg
     2521 gttccggcgg catggcgcgc ttgacggtaa acctgtttcg gtcggttccc gcggaccggc
     2581 ggcaccgcgt cgcctggacc gacccgaccg gcgaaacggt ctggctggcc gtccacatgg
     2641 actgaggata ttcgaacgcc cgggaccacc ccggcgcgtt cgctggatcg aaagatcgat
     2701 atttcggaaa gggtatggga taagcaaggt tatggacgaa gagaaacaag cgcaggtcaa
     2761 cacggaaccg gaagaagaaa aaagttttgc cgaactcctg gcggaaacct ctcccgatcg
     2821 cggccgtctc cggccgggcg agcgggtgga ggtgacgatc gtcaagatca cctcggaatg
     2881 gatattcctc gatttggggg gcaagagcga agggtatctg gaccggtcgg aactgctcga
     2941 cgccgaggga aacctcaccg tgcaggaggg cgatacgatc cgggcctatt ttctgtcggc
     3001 caagcgcaac gagaagctgt tcaccacgaa ggtgagcagc ggggaggccg cccgttccta
     3061 catcgaggac gcctggaaga gcgggatccc tgtcgaaggc gtcgtggaaa aggaggtcaa
     3121 gggcggctac gaggtccgca tcgggggatc gctccgggcg ttctgcccgc actcccagat
     3181 gggcatgccg cgccagcccc agacggaaag ccccgtcggc aagacccgca ccttccgggt
     3241 ggtggaatac gggtcgcgcg gccgcaacat catcgtatcg cgccggtcga ttctcgagga
     3301 ggaggaccgc cagcggaagg aggccctgaa ggacaccctg accgagggga tgaccattcg
     3361 gggaaccgtc gtttccctcc agaaattcgg ggcattcgtc gatatcggcg gcatccaggg
     3421 gcttttgccc atctccgaga tcggctgggg gcatgtggaa aaggttcagg acgtcctgtc
     3481 cgtcggccag gagatcgacg ccgtcctcct gaggctggac tgggccaacg accgcatcac
     3541 cctgagcctg aaatcgacac tgcccgatcc ctgggaaacg gtggagaaca ccttccccga
     3601 ggggtcggtc cataccggcg tgatcgcccg tctgaccaaa ttcggggcct tcgtgaccct
     3661 gtcgccggga atcgacggcc tcatccacat ctccaagatc ggcaggggga agcggatcaa
     3721 ccaccccaac gaggtcctca cccagggcca gaccatcgag gtgcgggtgg agaaggtgga
     3781 aacggcccag aagcgcatct cgctcacgcc ggccgccgat gccgcggcgc cgggaggaga
     3841 aggcgaccgg aacggcgatt accagcaata tgttcctaaa tcaaaggaat cattcggttc
     3901 cttcggagac atgatgaaag ggaaacttcc cgagcgctcc ctcgggaaga aggagacgga
     3961 ctgagcgggg tggaaataat gcttgacatc tagcgcggcc gtggtatggt cgtgccgata
     4021 taaagttatg aagagaggcg tgagggtact ggccctgtga ccgcccggca acctgatccg
     4081 taaggtgcca aagccagccc gcaagggaac catgaggtca taacgacaaa aggaatgaac
     4141 ctctccttgt gagaggtttt ttatttcaag ccaaggagct ttacatgcag atttcatcgg
     4201 aaagcgtaaa agtcggacat ccggacgtcg tggccgacgt gatctcggcc caagtgatcg
     4261 gccgcatcct cgatcaggaa aaggccctcg gcctgaccgt cgcgacgatg cctcattgcg
     4321 gtctcgaggt gttcctcggg aaaaacctct gcctgatcgg cggcgaggtc cgcgcccggg
     4381 tgaagatcga cgtggaggcg tgcgtccgcg aggcggtcct gtccctggga tacaacgacg
     4441 aggccgtcgg cctcgacggc cggaccatgc gggtcatcaa cgagatcatc ccccagtcgc
     4501 cggacatcaa ccagggcacc gccctcgaat tgaacaagga caaggagatc ggcgccgggg
     4561 atcaggggat catgtacggc ttcgcttgca acgaaacccc ggagctcctc ccgctgccct
     4621 acgtcctgtc cagccgcatg atgcgcacct tcgagacgtg cggcgatccc atcttcgccc
     4681 ccgacggcaa ggggcaggtg tcggtggaat acaacaccga ggtgtgtggt ccccggcggc
     4741 tggccaaggt gctgatgtcc aacgcggtgg actaccgcca gataaacggg ctgagccgcg
     4801 aggccatcaa ggcccgggcg aaggagatcg ccttcgggtg tctcgcggac tgggtggaca
     4861 aggacacggt attcctcttc aacccgaccg gggagtggca ggccgtcaac tcgtgcagcg
     4921 ccgcggattc cggcgtgacc ggtcgcaagc tcgtcgtcca gttctacggg ggctatcccg
     4981 gggcgcagct gggcggcggc gccgtcgtca acaagtcgcc ggagaaggtg gactgctccg
     5041 ccgccttcgg cgcccgctac gtagcgaaga acatcgtcgc cgccgggatc gccgagaagt
     5101 gcgccgttca gctctcctac gcgatcggca tcgcgcggcc cttctccatc tacatcaaca
     5161 ccttcagcac ctgcggcagc gcgacggacg agcgcctggc ccaggtcgtc gaggagctgt
     5221 tcgacctcag gccccagggg ctgatcgagc gcttcgacct cctgaacggc gacctctacc
     5281 ggcggatacc caagaccctg ttcatggacg actatccctg ggagaagacg gacatggcgg
     5341 ataaactgat gaaggcgctg gcctgaggaa acgcagaccc tggcacgaaa cccgggccgc
     5401 gggaagcccc tttcccccgg cccgggcgtt gtttggccct tcccgccccc tgccgcccgt
     5461 caaaacccga acggattgaa ccatgccccg gccgccgcaa aaagacacgg cgcgggcgca
     5521 gccccggccc cggctggaat cgaaaaaaaa tgacttttcg acgggacccg ggttatggaa
     5581 gggccgtaga cgcgagaccc cagattctct tttggaatcg gacccccgta tggatgaaga
     5641 cctcatcaag gcctaccggt tgtttctcaa ggacagcatc cgcaaggtca tcgatttttc
     5701 gcggacggac cagaaccgag gcgttgcgcc gccgcccgtc cagaagccct gcccaccgga
     5761 cgccgaacgg atcgacctga ccccttgcga tcggttcggg gccctgggga ggatcgacct
     5821 cgaaacggcg attcgaaacc gggaaagccg ccgctcgtat cgccgggaac ccctcgcccg
     5881 tgaggaattg tcgtttctcc tgtgggccac ccagggcgtc cggcagccgc cgttcgccgg
     5941 gcatgccctg cgaacggtcc cttccgccgg atgccgccac gccttcgaaa cgtatctgtg
     6001 tgtcctgaac gtccgggggc tcgccccggg gatctaccgc tatctgccca tggagcaccg
     6061 ccttctgctt gaattctccg aggagcgtct ggacgagaaa atccgtcgcg ccgctctcgg
     6121 ccagcaccat ccgggcagcg cggccgtgac gttcatctgg acggcgcttc cctaccgcat
     6181 ggaatggcgt tacggactgg cggcgcacaa ggtcatcgcc ctcgacgccg gccacgtttg
     6241 ccaaaatctc tacctggcct gcgaggccat cggggcgggg gcctgcgcga tcgccgccta
     6301 cgatcaggaa gcgatggacg ccctgctaaa aatcgacggg caggacgaat tcacgattta
     6361 tcttgcctcg gtcggtaaaa agctctaatc atccggcggc gggcaggcgg aagcccaacg
     6421 ccggggcgga ggcttgccgg tacgacggcg gtcggaaggg aaaggacacg tctcatgctt
     6481 ttttccgaac gggttttttg cggtcagttg acgttgggag aggtcggacg ccgggtgctt
     6541 ctggcgggct gggtggacgc cttccgggat cacggcggtc tgctgtttat ccatctgcgc
     6601 gaccgcagcg ggatcgtaca gatcgtattc agccctgaaa tagcggcagc cgatgtctgc
     6661 cggcgggccg ccgggctgcg cgccgaatat tgcatcgcgg ttcgcggcga agtcaaacag
     6721 cgccgtgagg gaacggaaaa cccccatatc gaaacgggat cgatggaagt gctggtcacc
     6781 gaactggagg tgctgtccga atcggatgcg ctgccctttg ccgtttccga caaggccatg
     6841 gtcgcgggcg ccccgtccgc gggcacggac acggtggccg aggacttgag gctccagtac
     6901 cggtatctgg acatccgccg gacgtccatg cgggacaatc tgatcgcccg gcaccgcatt
     6961 tttcaatgcg tccgagactt tctcgattcg cggggctttg tcgaaatcga aacgccggta
     7021 ctgacgatga gcacgcccga aggggcccgg gattacctgg tccccagccg cgttcatccg
     7081 gagaatttct acgccttgcc ccaatccccg caactcttca aacagctgct catgatcggc
     7141 ggtatggaac gttactttca actggcgcgc tgcttccgcg acgaagatct gcgccccaac
     7201 cggcaaccgg aatttaccca gctcgacatc gaggcctcgt tcatcgatga agaattcctt
     7261 tatgagctga tcgaggagct gaccgttcgg atgttcgccg tcggcggcat cacgctgccc
     7321 cggcccttcg cccgcatgac ccatgcggag gcgatggaca cgaccggctc cgaccgcccc
     7381 gatctccgct tcgggctgcg tttcgtcgat gtcacatcgg tgttcgccca gacccgctat
     7441 tcgatcttcc gccagattct cgagcgcggc ggctgcatca agggcatcaa catccagggt
     7501 cagtccggcc agctgagcaa gaatgtcctg cagaacgaat acgccaagga aatcgtgccg
     7561 tcgttcggcg cgaaggggat gacctggatg cgcgtcgaag acgggaaatt cgaatcgaac
     7621 atcgtccagt tcttcagcga gctggagctg gaggcgctgc gtggaaaatt ctccgcagcc
     7681 gacggggacg ttctgatcct gatcgccgat ccttcccatg cgacggtcac atcggcactc
     7741 ggccaactgc gcctgcatct ggcgaatcgt ctcggcctga ttccgtcgaa cagcttttgt
     7801 ccggtctgga tcacggagtt tccgctgttc gaggcgaccg atgaaggcgg cgtcacctcc
     7861 agccaccatc cgttcaccgc gccggaccgg gccgatttcg atccaaaaaa catcgaagag
     7921 cttctgtcgc tgcgatcccg ggcctacgat ctggtcatca acggcgagga actcggcggc
     7981 ggcagcatcc gcatcaatga ccgcgatctg caacgcagga tattcgccgc attgggattg
     8041 accgaagcgg aaacgaagga gaagttcggc tttttcctgc gggcgttcga tttcggcgcg
     8101 ccgccgcacg gggggctcgc actgggcatg gaccgggccg tttcgatgat tctcaagacc
     8161 ccgtcgatcc gcgaggtgat cgcattcccc aaaaaccgcg gcgccgcctg ccccctgacc
     8221 ggcgcgccct ccccggtgaa gcgggagcag cttgccgaac tggggctgct gaatctgggc
     8281 agccaggacg tcctgcccgg caccgcggaa aatgaaaacc ggatcgaccg cctatcctgg
     8341 gtgtcgcgca tcggtctgtc cgcggacgaa cgccccggga tggaagacgt cctggcacag
     8401 gcggacgcct tggccttgct ggcggcccag aacgcgggcg aaggagaggc gctctacacc
     8461 gtggccccgg tcgccaaccg tacgcgcccg ggaattcagg aaaaatgttc gccgctcgcc
     8521 gagacgggcc agctgctcag ccgtgcgccc gcggtcaaag gccgtttctt caaggttgcc
     8581 agtatcctgg aataaaggga gacctccgca tggattcaat ctttttctac cgccctcccg
     8641 aggccgccgt ccccgcagac ggaccgctca aggggatgaa catcgcgatc cagcccaatg
     8701 tctcggcggc aggctggccg acggacgccg gctccaacgc cctcgcccgt tttgtggccc
     8761 tggaagacgc caccgttgtc gagcggttgc gtcaggccgg ggcttcaatc tgcggctcga
     8821 cccgcatgag cgaattcgga ttcggtctcg acggcagccg ggccggagag gcgatcaaac
     8881 ggaacgccgc ccatgcggag ctggttctgg acctgatggg cgagagtcgt ctggccgcct
     8941 cggcgggcga cgtatgcggt ttcaagccca gttacggcct ggtgtcgcgc ttcggtctca
     9001 tcggtctgat tccatcgatg gagtgctacg ggattctttc cgacagcctg aaaaatatcc
     9061 gcgagattct gaaggccatc gccgggccgg acgagcggga tttctccctc cccgatgaaa
     9121 cgaccccgga tttttccccg cagaacatcg acacgggaaa aacaaccgtg gggatcatca
     9181 aagaggcgcc gggcagcttg cctgcccggc aggaatctct ttaccgtgcc gcaatcgaaa
     9241 cgctcgggga tgcgggtttg accgtcaagg agctgtccct gcccgatttc ccgctttttt
     9301 tgccggtcca taaaatcgtg ggcgccgtgg aagcgtcctc ctgcaccgga cggtacgatt
     9361 cggtccgcta cggcgtgcgc gtgccgggcg ccaaaaactg gaacgagatg tatctctcct
     9421 cgcgcggtgc ggccttcggc ccgctgttga aaagctatct gtttcagggg acgttttttc
     9481 agttcgagcg ctacgacgcg tatgaagacg cctgccgtat ccgcgcgcgg ctcgtggccg
     9541 gcatgcggcg gctcgcggcg caggcggact tctgggccat cccggtggaa agaggcggcc
     9601 cggacgcccc ggcgtcgctg gccgatacgt atgcgcaatt cgcgctcacg gcctatgcca
     9661 atgtcgccgg cctgccggcg ctttacctgc cgccggcgca aggccgacct cacccgggcg
     9721 tgcagctgtc gggtccgagg ctcagcgacg cgcggctgct cgcgctgggc gagcaccttc
     9781 taaacgcgcg ccggggaggg aatgcatgat ggaatttgaa gcggtcatcg gattggaaat
     9841 ccatgtggag ctcaactgcg cgaccaagat gttttgcgat tgttccaacc gcccgggcga
     9901 caaacccaac atgaacatct gtccgacctg cctgtggttt ccgggcgccc ttccccggct
     9961 gagccgggag gcgctcgaaa aggccgcgct cctgtgtctg gggctggggg cggaacttca
    10021 gcccagaagc gccttcgacc agaaggttta ctactacccc gaccttccga aaggctatca
    10081 actgtcgcag gcgcaccttc ccctggcgcg tggcggcgcc atcgacatct cggatgaaaa
    10141 cggccggccc aggaaactgc gcatccacca catccacatg gaggaggacg tcgccaagct
    10201 ggtgcatgaa atggagggtc gcctgccgat cagcctggtg gatttcaacc gtgccggcgc
    10261 gccgctcgtg gaaatcgtga ccgaaccgga tttccgcacg ccctccgagg cgatggaatt
    10321 tctcaaggcg ctgcggaccc aggtgcgtta cgtggggtcg tccgaatgca gcatggaaaa
    10381 cggtacgatg cgcgtcgatg ccaacatttc cgtgcgcccg aaaggcaccg gccagatgaa
    10441 tacgaaggtg gaagtcaaga acatgaattc catccgtcac gtgggtgatg ccgtcgccta
    10501 tgaaatcaac cgtcagagcg cggacgtgca ggcaggcaaa gtggtgaccc tgcatacccg
    10561 cctctgggac ccggacaaga aagtcacgct ggccatgcgc gggaaattcg aagggccctg
    10621 cattcccgac cccgtcgtgc cggtcattga actggcgcct gagtggattg aacggatgcg
    10681 cacgcgtctt ccggaaatgc cggccgcccg ggccgagcgc tttgtctcgc agcacggact
    10741 gtccggcgag gaagccgtct tcctgagcgc cgatccggag gtggccggtt attttgaggc
    10801 gctgatcggg cagggggtct cgccgcgcac ggcgatgcac tggctcgtca cgcaactgat
    10861 cccggccgtc aaggaccggg ggcaggagtt gagcggatcg gccgtgcccc ccgcgcgctt
    10921 cgccgccctc ctgaacatgc tggcgcggga cgaaatcaac gcccatgcgg cgcgtgaggt
    10981 cctggggcag ttgttcgaca gcgacaaagc cccggaggcg atcgtcagcg ccctcggatt
    11041 caggcaggtt tccgaccccg atgccctcga tgcgctgatc gacaaggtgt tggctgagca
    11101 accggcggcg gtcgccgacg ttcgggccgg acagggaaag gccctgggct ttctgatcgg
    11161 ccaggtgatg caggcctccg gtggaaaggc caatccgaag gtcctccggg atttgctggc
    11221 caagaaggta agcgcagcct cgtagacggg ggggggcgcg ggggctcccc taacgaaccg
    11281 gggcgcccgt cttcggatcg cggtcgaatc cgatcagcgt caggccttcc ggtgtatccg
    11341 tcgccaggag catgccgcgg gattcgacgc ccatcagttt ggtgggtttc aggttggcca
    11401 cgacgacgat cttcttcccg accagggtct ccggggggta atccttcgcg atgccggcca
    11461 cgatctgtcg ctcctcgccg agatcgatct tcaggcggac gagcttgttc gacttcggga
    11521 cggcctcggc ttccaggatc gtggccaccc gcagatcgac gcgtgcaaaa tcctcatagg
    11581 cgatctccgg cttgaccggg accatctcct ccttcggttc ggacatcggt acggcctcct
    11641 tcctgaattc aaccctgggg aacagagcct ctccccgcgt aagccggctg ccggccggca
    11701 ggccgcccca gcttcggatc gaatcgaaat cctgcgtccc gccttcgatg ccgagctggg
    11761 agaggatctt ctccgccgtg gacggcatga agggggcgat caggacggca atcacgcgca
    11821 aggcctccag gacgctgtac agcaccgcct ccagccgccc ccgccgttcc ggctccttga
    11881 ccagcgtcca aggctcgttc tccacgatgt acttgttcgc gacattgatg aactcccaga
    11941 tgaccatcag ggccttgtgc agacccagct cggcaaagtc cgcctccacc tcggcgagga
    12001 tccgccgggc ggtttccgga aggggcgacg cctccgcctc tgcctccgtc acggccggcg
    12061 cctggccgga caggaatttc gccgccatcg tcagtgtacg gctgacgagg ttgcctaggt
    12121 cgttggcgag atcggcgttg atccgctgga caaaagcctc ctcgctgaaa ttcgagtcga
    12181 gaccgaagac catgtcccgg agcaggaagt agcggaacgg atcgagcccg tatttgtcct
    12241 tcagatccag cggcttgacg acgttgccca ggcttttgga catcttgctc gcgtccacgt
    12301 tccagtagcc gtggacgttc agattccggt agggttcgat cccggccgcc ttgagcatcg
    12361 tcggccagta gatcccgtgg ggcttcagga tgtccttggc gatcaggtgc tgggcgacgg
    12421 gccagaactc gtcgaaacgc gccccgtcgg gatagcccaa cgccgtcacg tagttgatca
    12481 gggcgtcgaa ccagacgtag gtgacgtagt cggcgtcgaa aggcagggtt atgccccacg
    12541 tcaggcgtga cttggggcgg gagatgcaca ggtcctccag gggttcgcgg aggaaggcca
    12601 gggcctcgtt gcggtagcgc tccggccgga tgaactcggg gtgcgcctgg atgtgcccga
    12661 gcagccagtc ctggtatttg ctcatccgga agaagtagtt gctctccttg cggtactcgg
    12721 gcgcggtctg gtggtcggga cacttgccgt cgaccagttc cttttccatg tagaagcgct
    12781 cgcacccgac acagtagaag ccctcgtagc tgccgaaata gatgtcgccc gcatcgtaga
    12841 ccttctggag gatccgttgc acggtccgga cgtggtcggc gtccgtggtg cggatgaagt
    12901 aatcgttcgt cacggagagt tccggccaca gggcccggaa ctgggcacta atcccgtcgg
    12961 cgtaggtctg gggggtgacc ccggccttct gggcggcctc ggcgatctta tcgccgtgct
    13021 cgtcggtgcc ggtgacgaag aaggtccggt atccctgggc ccgatggtac cgggcgagta
    13081 cgtcggccac gatcgtcgtg taggcgtggc cgatgtgggg agaggcgttg acgtagtaga
    13141 tgggggtcgt gatgtagaag actttttcca taatggagat cctttgaatg cttgaaggcg
    13201 cgcggccggc ccgaagacgg gacaagatca gagatccgtc acgttcaggg tgatttcctt
    13261 gccgccctcg agcaccacgg tgatctgccc ctcgaagacg ttctggcggg tgacccgtcc
    13321 gcgtccctcc ggggtctgga tcaccttgcc acacttgggc atgtcgcgct tcatgtcgag
    13381 gtaggtttcg tgttcgaagg ccaggcagca catcagccgc ccgcaaagac ccgaaatttt
    13441 ttcgggattg agcgacatgc tctgttcctt ggccatcttc accgaaaccc ggtcgaggtt
    13501 ctggaggaag gtggcgcagc agatctcccg gccgcagacg cccagcccct tgatcaggcg
    13561 cgcctcttgg cgggcggcga tctgccggag ctcgatgcgg gaacggaact tctgcaccag
    13621 gtccttgacg agttcccgga aatcgacccg gttttccgcc gtgaaataaa agatgacctt
    13681 gctgcgatcg aagaggcatt cgacatcgac gagcttcatg ggcaggccgc gttcccggat
    13741 ccggacgaga cagaaggcct gtgcctcgcc ctccagacga tcgttgtccg ccttgatttt
    13801 ctggtcttcg ggcgtcatct tgcgcaggat gttcttgagg gtggccggaa ccttgggcag
    13861 gggcacctgc cggacgtcgg tcgcgaccca ggccagcgcc ggaccgttct ccgtctggat
    13921 catgacgggg tcgcccttcc tgagctccag gtccccggcg tgaaaactgt agattttgcc
    13981 ttcctgcttg aatttgacgc cgacaatgtt tcgcaacggt ctttaggtac cccttttcgt
    14041 aagatccgcc ttcagagagc cagtctaaac atcatgactt ccaaagtcaa ttgcttattg
    14101 gcgttctgct ccagggccgc gatggcccgg tcgatgagcc ggatgttgtt caggatctct
    14161 tcccccgtgc gccggtcggc caaggtgcgg atttccgcct cgcagtcccg gtggatgagc
    14221 aggtccgccg ctcccgtctc ccggacgacg agggcgtccc gccagcagct ccgcatgacg
    14281 gccagccttt cagccagcga acggcgctct tggccgagat cgccgaggaa ggacaggcgc
    14341 tgccagggtt cccggagggc gggggaggcc atccgctcca ggagggcccg ccgcatctcg
    14401 agaaaatccc cctgccgcag gttcagggcc ctggcgatgc tgccaccgga acaggaggca
    14461 aggaggccgg ccgtcggcgc ctccagggcg tgccgctcca cgagaaaccg ctgcaccgtt
    14521 tcccggggga gaggatgaaa ccgcacctgc tggcagcgcg acagaacggt ccggggcagc
    14581 cggtcgggat gcgccgtcag gagaattagg ccgtgcttcg cggacggttc ctccagggtc
    14641 ttgagcaggg cgttggccgc ttcaaggttc atccggtcgg cctcccggac gaggaaaacc
    14701 cgccaccgtc cctcgtaggg cttgaactgc atctgttcct ggagggcgcg gatatcccgg
    14761 attcggatgt actggccgtc ggccgtgagg gtggacacat cggggtgcgt gccgtgcccg
    14821 atcttccggc aggaggggca tcgcccacag gcgtccgtcc ccccttcgcg gcaattcagg
    14881 gcctgggcga acgccagggc tgtcgtccgt tttccgatgc cctccatgcc gtgaaagagg
    14941 agggcctgcg gtatccggcc ccgttcgagg acggtcttca ggatgccgag gggacgttta
    15001 tgtccgtaaa tgtcacggaa gggcatgggt ctgacctttg atgaacgcgg ccagctggac
    15061 ggagacatct ttgtggatgg tgaaaatgtc cctggtggcg tcaatgatgc gaaaacgctc
    15121 cgggttctcc cgggccagcg tcaggtagcc ttcccggatc ctccggtgga acgcgagggc
    15181 ctcgttttca aaccggtctt ccggcgcctc tccgccggca cggctgatcc ggtcgagcgc
    15241 ccgctgcaat ccggtttcca caggcagatc gaacagaaac gtgagcgccg gacgaagcgg
    15301 cgaggcaaag gcgtcgatcc gccggatgca ttccaggtcg agcccccgtc caaacccctg
    15361 ataggcgagg gaggcgtcgc tgaagcggtc gcagagcacc cattcccccc gccggagcgc
    15421 cggcagaatc gtctcccgga catgctgggc gcgggcggcg cagaacaaaa ggatctccgc
    15481 ttcgggacag atctcctccg ggctctgctg gagcaggatt tggcggattc gccggcccag
    15541 gggactcccg ccgggctcct cggtcagaag atgggggaga tgatgattgc gcagcacctc
    15601 acgcgccatc ttcatctggg tcgtcttgcc gcacccctcg atcccctcaa atgtgatcaa
    15661 tggctgcatc ggggtccgtc cgttcccgct gaaaaaaaga ggggggaaac aaccgggcgg
    15721 gtctcgcggt aatttccccc tgcgtcacga cctgtctgat gccggtctat ttgtcgtcgc
    15781 ccggaacgat cgcctccacg ggacactggt ccgcacacgc gccgcaatcc gtacagagac
    15841 tggcgtcgat gacatattta tcgtcgccct cgctgatcgc ttccaccgga cactcatcct
    15901 ggcaggaacc gcaggcaatg cattcatccg tgatgatgta cgccataccc tttattctcc
    15961 ttacccttgt tatcgtattt gccttctgcc ggtccgttgc cccggaccgg gatcttcaga
    16021 acggccggac cggcctcgct gtttcgtttc gcgcggcccc attcgcctaa gcgacggaca
    16081 cgacctcctt gacaccgggg acttcctgaa gcaggagccg ctggatcccg ttcttgagcg
    16141 tcatctgcga catggggcaa ccctggcagg cacccgtcag cttcaccttc accacgccgt
    16201 cgtccgtgac ctcgaccagt tccacatccc cgccgtcggc ctggaggttg ggcctgatct
    16261 tgtccagagc ctgcgtcacc ttctctttca tcctgttctc ctcccgatac cccgtccgcc
    16321 ccgatcgcgt gttttccgcc tgccctgccc ggccggatct ccgcggtaaa gccgagggcg
    16381 ggcggacctc aatggggcat gtattcgatc atctccttga ccaggtccct ggcgacatcc
    16441 aacagtttcg gcttgaagac gcgatatttt tcaatggcga catgtccggc tttccacatg
    16501 atcttccctg tggtgacgtc gatcagtttc atccccatcc ccaccttggc caccttttcg
    16561 ccgttctcga ccgcgtagtt ccaatcgtcg acgttcacca tcaacagggc gtccacctgg
    16621 gtcagttcgc cgattctctt gctctgctgc gggtcggaaa aattgacggt cttgagcttg
    16681 gccatgtagt ccatcacgac cttccggagc tcctcgttgg cggaaaactg ccgggcgatg
    16741 gtatccccgg agaccacgtc ggcgaaccac tttttgtcaa tcagaacgcc cgccagggtc
    16801 tggtccacat tgccccgggc ctcttcgtag gtccccacgt ccaccggcag gaccccgatc
    16861 cgccgcggat ggaagtactt ggattccgga tcaacctggg agtaacggag ccctccgcaa
    16921 ccggcgagag acaggacaag cagagccgcg atcagcatga gccctgtttt ttttctcatc
    16981 atcggcgcac ctccttcaac gggacattac aaaaaggccc gggggaaaaa caggcccacc
    17041 ccggcgctca aaccggcaac caagtaaacg gattccagca ggccttccag cagaacgccc
    17101 ggaaaccatg cctccctatc acaaatggta aaacaaatcc atacataaaa tggacagctc
    17161 atgagcgtcc agatccgcca gggggcccat cccgcgaacg gcgccgcgac gaggacgagc
    17221 gcggtcagga gggcgatgcc cttcaggaga accagggtgc ggtccttgcc gatcaggacc
    17281 gggatcgtct cacggccgat gaggcggtcg ctctggatgt ccaggatatc ggacagggcg
    17341 gaacggacga acaccaggga aaaggccaga cagaacgcga ccatcaggga cggcggcgcc
    17401 tcgaatccga ccgcgatccg ggggaggaag gcggtcaccg cggcccatgc cagggccatc
    17461 gccacgttct tggaacccgg caggtcgcac aacccccgga agcgccagtg cgacggcaga
    17521 atccgtagat tgtagacgag gcccaacgcg gacagcgtga aaagcaggat gaacggggcc
    17581 caccccgaaa ggcacgcgag cgtcagcgcc agcaccatgg cgagcaccgc cagcgaaagg
    17641 aagagtcgtt cgtggcgccg gtaggtggcc tcccggaagg acccgatgat gctccccttc
    17701 cgggcgagaa accggttcag cgtatgcatg gcgtagacat aaagcgccgc cgtcagaaga
    17761 ttcaggacat cgggccggag gccctgcagg aacatgcagg cgagtgcaag gccggcggca
    17821 ccgatcgcgg agaagacctc cgttcggacg gtcagcgtcc agaaggcgaa caggccccgg
    17881 cgcttctctc cccggtcgtt ttcgaggtag tcgaccacgc gatggatgat ccagttcggc
    17941 gtcgaggcgc cggccgacac gccgattcgg cggtagcgag acaggtcgac cccgcccagg
    18001 tcttcggccg tctcgatgtg aaacgtcggc gtcccctggc gttccgacag gcgcgccaga
    18061 cgctgcgtat tggcgctgtt ctgccccccg acgatcagca tggcctccat ctccgccgcc
    18121 atcgtccgga cctcggtctg cctcttttcc gtcgagtcgc agatcgtgtt gaagaccacg
    18181 gtttccggaa accggtcccg gatgtccccg acgagagtct cgaactcctc caccccctgc
    18241 gtggtctggg ccacgacaca caccttgtcg agggtcggca agcgactgac gtcctccgcg
    18301 ctgctgatca ccgtcccgcg gtccccggca tagcccagca ggccgttgac ctcgggatgg
    18361 gaccggtcgc cgatgatca
//
LOCUS       DAOOLJ010000033        18358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3123_length_18358_cov_15.622630, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000033 DAOOLJ010000000
VERSION     DAOOLJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 18358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18358
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_18358_cov_15.622630"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1784
                     /locus_tag="PLW16_06700"
     CDS             213..1784
                     /locus_tag="PLW16_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB70630.1"
                     /translation="MSAGLIDFFHEATLRICGSLEIDVVAKDCLEFLQEFIPLDGISV
                     HYYDEKNRSLLLLATASNIPLDVSHPSIPISLEGHRSFKNTKSTVVIYNNPEEDPVPM
                     KIWETLGFIDKSSLVLFVNFKGTRLGQIDFFCKGRNRYTEEHARLIAQLHNPFSIAMA
                     NSLQYREIVNIKRLLADDNRYLRQELRQVASGEIIGAGNGLKSVMEAVEQVAPLTSQV
                     LLLGETGVGKEIIANAIHYASPRKDGPLVKINCGAIPDGLIDSELFGHEKGAFTGAIS
                     QKLGRFERAHKGTIFLDEVGDLPAPAQVRLLRVLQEREIERVGGTNPIKVDVRVIAAT
                     NRKLEDLISSNRFREDLWFRLNVFPIRIPPLRERKSDIPRLVLHFILKKSRELNLAVD
                     PVLAPGAMEKLMAYDWPGNVRELENIVERALIRMRTGHTYRPLTFDEFPMDPPSARPA
                     EREFPSGPKEAPCHLDDAVRQHIQEMLDRTGGRVQGAGGAAERLGIKPNTLRNRMRKL
                     GIPFGRKSHRGTPGD"
     gene            1878..2411
                     /locus_tag="PLW16_06705"
     CDS             1878..2411
                     /locus_tag="PLW16_06705"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB70631.1"
                     /translation="MLDVEKYGKQTVHDLLLKHLIVINAGWFKFENLIRATHPEVLAG
                     NGYVQIMEELCSDQARGLARVLGVQGDGIDSMIELLRCSQWGVFERFEIERLSETSCL
                     MRIMGCSTQRAARKWGMDHYACAEATMVCLGSFCKQVNRDATIHKIFAPPEVDPVGNR
                     GQVSCEWVISIDGTPGV"
     gene            2479..3702
                     /locus_tag="PLW16_06710"
     CDS             2479..3702
                     /locus_tag="PLW16_06710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70632.1"
                     /translation="MEQTAFRVYRYRWVVLLVYVLLNALMQVQWITFAPITSEAVAFY
                     GVPAIQIDLLSLIFMIVYIFVSLPASYAIDTWGIRIGIGIGAVLMGVFGFMKGVCGSS
                     YALVAVAQVGIAVGQPFVLNSVTKVGVRWFPLRERATQAGISVLAQFVGIILAMVLTP
                     LLFKKYGMETLLMIYGVVTLAGAVIFLIFNRERPATPPCPRGQDERIAVLAGLKHIFR
                     QRDMIYLLLVFFIALGIFNAVTTWIEQIVRPRGFDITQAGVTGALMMSGGILGAAILP
                     YFSDRLRKRKIFIVATMILAIPGLAGLAFAGSYELLLASSLAFGFFLMAGGPIGYQYS
                     AEICYPAPEATSQGLLLLAGQISGIVFVIGMDVLAAGSDSKTLAMIVFLVLMALNVFL
                     TTRLRESTLVKSGGI"
     gene            3841..5472
                     /locus_tag="PLW16_06715"
     CDS             3841..5472
                     /locus_tag="PLW16_06715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB70633.1"
                     /translation="MKRTDKYVLAVDLGTSGPKTALVSVHGDVVDSEFQDTPVILFPD
                     GGAEQDPRGWWDAIRSTAKKVIGKNRVPPEDIAAVSVTTQWSGTVPVDRDGRHLMNGI
                     IWMDCRGSESVKDILKGFPKIEGYPLFRLLVWQRLTGGAPARSGKDPICHILWLKQNH
                     PDIYRRTYKFLEPKDYINLCFTGRFAATYDSILLHWVTDNRDPSHVVYNDRLLKMAGI
                     EREKLPDLIQAMDILGPVKSEVARELGLREGTPVIGGTPDVPSAGVGSGAVRDYEGHI
                     YIGTSSWVSAHVPFKKTDLAHNFGSFPSPIPGRYIILTEQECAGKCLTWLRDNILYHK
                     DELLVEESVPDIYKVLDKIVARVPPGANRILFTPWLYGERSPVDDCTIRASFFNLSLE
                     NTREDIARAVFEGVAFNARWLLAPVEKFMGRRFDYLNFIGGGANSDIWSQIFADVFDR
                     KIRQVKDPIYANARGAAFLASMALKYMTLEDIPRTLKIKAEYTPNPENRKLYDERFNE
                     FVHYYKTQRKACARMNKNAAGVCVPPLVSQSNVGR"
     gene            5480..6889
                     /locus_tag="PLW16_06720"
     CDS             5480..6889
                     /locus_tag="PLW16_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB70634.1"
                     /translation="MEYSEIMKRKDAILERPVRSIPPDKLAEEHRRYAERCKRSIEIF
                     EKAKGLIPGGVEHNLSQNKPFPLAMDRAKGYRAWDVDGNEYIDYLMCGAPIMLGHGYE
                     PLDEKIIGIIREKGPATGLTSEYELLAAEEIIRHMPGVEMVRFLQSGTEADMAALRIA
                     RAFTGKLKIIKIGGSYHGWSDQVVYSVHIPRTGNFESKGIPPECYAHTVDVAPNDFEA
                     LEAAFRDNQDKGGVAGVMVEPIGGESGAHPVHPDWNRTIRKLCDRYEALMIFDEVVTG
                     FRLDMGGAQKLFGILPDLTVLGKIITHGYPSSGAVAGRRDVMKVCAGSMGEKIFVGGT
                     LAANPISMAACYYALKYMEETGAVAKATAYANRLTQALNDLYATRPDLGFFVYNFGAI
                     LHYMTTAFFSVDLNAPDALTQIFSRKQVADDYQVVTMARGLCTLAGTRMYTCMQHDDE
                     ALKKTLGVWEYLLSLIPRG"
     gene            6939..8282
                     /locus_tag="PLW16_06725"
     CDS             6939..8282
                     /locus_tag="PLW16_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HPB70635.1"
                     /translation="MKKNQGPKVVKKQIGPYADKPIALPEGIDMENHVVATYWIQADK
                     GWDMSIMGQVMAIEQTTGTWVPVPGETPEVRAGHVAKVIGVYEAPYYEYGLPADVTER
                     QYILQIAFPAANVEDQLPMMLTATIGNISMVPNFKLLDLRLPKATLDQYQGPKFGTDG
                     WWKALGLKKGRPLLNNMIKPCSGYPLEVGADLFRQAALGGCDVIKDDELIASMRYNDA
                     VKRVKTYMKIEREVYEETGEHTLYTVNVTDRLPRMFDLARRCVDAGVNGMMVNYLAVG
                     PEAMRALAEDPAINVPILAHMDLAGAYYMSPNQGIASPIILGKFPRLCGADAIVLPFS
                     MGGKAMYMHDRFMNTVRNLTYPMGRLKPTMPMPSGGITPANVADIVAAVGRDIMIGSG
                     GGIHAHPQGPRAGARALRQAIDAAVKGIKLELYAKEHEELGVALGVWSKKTDFKI"
     gene            8408..9469
                     /locus_tag="PLW16_06730"
     CDS             8408..9469
                     /locus_tag="PLW16_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70636.1"
                     /translation="MDDYLHDDPRLPAAWAPWFSHEYLSYFCVRALDGLMALPPVPVR
                     PNPRFGDRIWPIGPRHIDVYKESYRAHRQLDPLWSFDYKKRNGDFALEPPAGKMIEPW
                     KVLVIYSTEPDLYPDYDLILHRNQKITGGSHGWRHMRFRLLGMTFGIAPRSFSVHRDL
                     ARLAFENNHDYWGWRYLSRGAHYLADLGHPFHVKALPGSFLLKKILARHELFRIVSAV
                     HQSYEVYTERRFREGFEPFKEALLRGAREGRDTPGDVRDLLPAYIKRAERRHNPIFYF
                     FFDRFGPELIEAFSRMNPDSPLDAATQTNRCSADAARVIFKDTHRDKLDFLDRITVEI
                     LADVGRMLGMLFAAFSPKS"
     gene            complement(9489..11318)
                     /locus_tag="PLW16_06735"
     CDS             complement(9489..11318)
                     /locus_tag="PLW16_06735"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB70637.1"
                     /translation="MNGKSSLERLIKTKHSRRRFLAFTGKSMALLAALPGLGLLRGTA
                     NAKPTAGKIPGRGVVTGTGGHDTLLRNGFIVDGSGKRGFKGHLLIRDDKIERISRDEI
                     RASCPVVDCTGQVIMPGIIDAHSHMDWYLPIRGRDDLKLPFTAQGVTTFVAGHCGYGV
                     AGFRKNSPHMNLIAPRTKGLFRIEWDTMAQYFDYIEKTGLTHNLVNLAGHGTTRTSIR
                     GFSAAPMNRDEMKEMLALLEQSLDEGAVGVSLGLQYEPGIFAQTAELREIARLVKRKG
                     KILTVHLRAYSALAPGYPMSVPKILLDYIMPFDGYEPHNLIAIDEMLNIARETGVRLQ
                     ISHLIFVGTRTFKTCEDALKRIDRAIRRGVDVKFDTYAYHCGQSVINVIFPAWFLAKV
                     PQAYDDPKMLARLRDEWQQTMRFLGFGVKDIQITYANHPELNRFNGLFLADISKKRGM
                     DWFENTMDFARKSKGLANILNHGYSNLPVVEALLASPAALFMTDALPAPQGVQNPAMF
                     GNFPRFFQLAREKRLIPLEEAVRKMSGATAERYGIKDRGFLRKGLAADITVIDWNRIR
                     DNNTLTETSQAPTGIAAVFINGRRVLNQGKVDGSIRAGRILPA"
     gene            11635..12036
                     /locus_tag="PLW16_06740"
     CDS             11635..12036
                     /locus_tag="PLW16_06740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB70638.1"
                     /translation="MYRKLLSPRISEINGAGHVGHNVIPVWLEEGFLEIIRLFNPELR
                     GKPGDPFLVMANVNIDFVNEIFLGEDVEIVTGVKKIGNASFVLIQQIYQSGKLCARGR
                     PTFVYLDPQSRKSIPIPPPIRRVLEKHVVEA"
     gene            12268..13749
                     /locus_tag="PLW16_06745"
     CDS             12268..13749
                     /locus_tag="PLW16_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPB70639.1"
                     /translation="MTSARIKGHGIAGGLFVLLFLALLAVRLDVFPGALLPGSGPAAS
                     SAGPSRADEDRWMAVYQSGRKIGYSHRRMQRVPRGYCFEESGLLRINTMGVVQDIRMK
                     TTGDLNPDGTLSAFRFDLQSSLFSFVVRGEVRGRDAIVYQGPPSAEKQIRIALREPPH
                     LSNSLYEALRGKALATGQEMTLNVFDPALMAERPVRITVLERETIQIMGRPHAARKVE
                     VDFMGARQAAWLGEDGGVLREQGLLGMTVEQVTKDRALDGLTLAASADLTDAASIPSR
                     VRLDAPETLAHMTVRLANVRPESLLLDGGRQRYEQGRLTVVREAMPAARTTRDALPAE
                     MRPFLQATPFIQSDHPEIRARAARIVSPGDGDAVKARKLVNWVYRNVEKRPVLSVPNA
                     LETLTHRVGDCNEHAVLLAALARAAGIPAQIEAGLVYLRGRFYYHAWNVLYVDGWVTA
                     DATMGQMPADVTHIRLVRGEADRQIDLAGIIGDVDLEIVDTKK"
     gene            13746..14480
                     /locus_tag="PLW16_06750"
     CDS             13746..14480
                     /locus_tag="PLW16_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70640.1"
                     /translation="MIELTDLTKRYGKTLAVDRLNLSVAPGEIFGFIGPNGAGKTTTI
                     RMMGGTLSPTAGAVVIDGIDMAARPEEAKRRIGFIPDRPFLYEKLTGMEFMRFSADLY
                     GVGDGTFLQKAEELLRTFGLFDWSHDLIEAYSHGMKQRLVISAALLHNPRVIVVDEPM
                     VGLDPAAIKMVKDILKDLARRGTAVFMSTHTLSVAEDLCDRIGIIHRGALIALGTTRE
                     LKRLAQAQQADLEEVFLKLTEEQALS"
     gene            14477..16174
                     /locus_tag="PLW16_06755"
     CDS             14477..16174
                     /locus_tag="PLW16_06755"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70641.1"
                     /translation="MNGVLTLLKPRLWSFLNGNVARDREPRNIKWLLFGGIGFLFWAG
                     IFTVFYRVLRYFQGVEGFGDILAYKLLSMVLITFFALLIFSSIITALSKFYLSRDLVL
                     VHALPVSPAQIFLARWIESTIDSTWMVVVYSLPIFLSYGIVYRGGILFYATILGALLP
                     FCLIGSALSAIAVVLVARFLPAGRIRTVFVFLSLVLFVILLIAFRMMRPEQLTNPDTF
                     ASVLLYFRSLETPGSPWLPSTWIYDSLREVLSGNRSGALFHLALLWSGAAALGYLMTW
                     IAGAAYFQGFSKAQTTAERLLPSRQGRGAAGAWLLGFPSGPTRAFAVKEIKTFTRDQT
                     QWPQIFLMIALIVVYLYNFSVLPLDKSPIKTFYLQNLFSFLNMGLAAFVLTALSARFI
                     FPAVSTEGVAFWIVRASPVSIRSFLWVKFWVYFLPLAILAEFLIVATNLLLQVTDLMM
                     ILSTATVLLMVPGIVAMGVGLGAAYPDFQSENPAQAVTSFGGLLFMILCAGFIGFIVI
                     LETGPVYSVFMASLNGRGLALLQWVWLAISFALVILLCVLAVVLPMRLGERRLKALA"
     gene            complement(16251..17417)
                     /locus_tag="PLW16_06760"
     CDS             complement(16251..17417)
                     /locus_tag="PLW16_06760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB70642.1"
                     /translation="MQDFTFFLPTRVVFGCGKVRQVGQECRMLGGKKAFVITGTTATA
                     TSPGFAAALESMREADLPFEVYDRIEHDPTLEAIETARQTALKSGADLIVAYGGGSPM
                     DAGKAIAVFATNKGAVEDYLYVKKVFKLPGLPFLAIPTTAGSGSEVTAAMVIRDDERK
                     QKTGLSHPLAFAKTAVIDPETHVSMPPTVTAATGMDALTHAIEAYTSKSHQPFSDAFC
                     LQAIRLIGENLRRATGNGTDLAARSGMAAASTLAGVGFTFAGLGSVHGLAHAIGARFD
                     VPHGVANALMLPYVMEASIIADMPRFRDIAAALGENISGLSLRDAAFAAVDAVNILKA
                     DLDIPRCLTDVGVRDRALKDITEDALAYRLRPLAPRDFCRADFQRILNRAMGKP"
     gene            complement(17544..>18358)
                     /locus_tag="PLW16_06765"
     CDS             complement(17544..>18358)
                     /locus_tag="PLW16_06765"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70643.1"
                     /translation="ATACLREGLVQGIRISTRPDEIDDDTAQFLRNAGVWTVEIGAQS
                     LVDEVLEKSRRGHTVADVVRTVARLKTKGFATGLHLMIGLPGEDGAAFAETVRRAVAL
                     QPDMVRLHPTLVFRGTELADLYRAGAYAPLSLENTLAACRNALGAFENAGIPVIRLGV
                     QTTPEMEAPGAVLAGPYHPALRSRVEESIAGEMAARLLAGLPVRHRTILFTAAPTLAA
                     HLKGRKKAQLRALQKDFDLAGVEVAVDPASPGNVLTLTVGDRTERTGRWGPL"
BASE COUNT         3331 a         5588 c         5914 g         3525 t
ORIGIN      
        1 tcagggttgg tttgattgat acgcactcca aaaggctcct ttcgttatta aatggaagct
       61 taaaccgtgc catgaacaga taatgtgtat tttcagatag atatgatttt cagcccggtt
      121 ggactgcata aatgctgtag ataaagtgaa ttatatggta gttatctccc aaaatatgaa
      181 tgcagcggga tccgacgacg ggggtttcaa cgatgtctgc cggtttgatc gacttctttc
      241 atgaggccac gctgcgcatc tgcggcagcc tggagatcga tgttgtggcg aaggactgtc
      301 ttgaattcct gcaggagttt atcccgctgg atgggatctc ggtgcactat tacgacgaga
      361 agaaccggtc cctgctcctg ctggccacgg cttcgaacat acctctggat gtttcgcatc
      421 cttccatccc gatctcttta gaaggacatc gatcgttcaa gaacaccaag agcaccgtgg
      481 tcatctacaa caacccggaa gaagaccccg tgccgatgaa gatttgggag acccttggct
      541 ttatcgacaa atcgagtctg gtgctcttcg tgaatttcaa gggaacaagg ctggggcaga
      601 tcgatttctt ctgcaagggc cgcaaccgct acaccgagga acacgcacgg ctcatcgctc
      661 agcttcacaa tcctttttcc atcgccatgg ccaacagtct ccagtatcgc gagatcgtca
      721 acatcaagcg actgctggcc gacgacaacc gctaccttcg ccaggaactg cggcaggtgg
      781 cgtccggcga gatcatcggc gccgggaacg ggctgaagag cgtcatggag gcggtcgagc
      841 aggtggcgcc gcttaccagt caggtgctcc tgctgggaga gaccggtgtg ggcaaggaga
      901 tcatcgccaa tgcgatccac tacgcctcgc cgcgcaagga cggccccctt gtgaagatca
      961 actgcggcgc gatcccagac ggactgatcg acagcgagct cttcggtcac gagaaaggcg
     1021 cctttaccgg cgccatctcc cagaagctcg ggcgtttcga gcgggcgcat aagggcacga
     1081 tctttctcga cgaggtcggc gatctccctg ccccggcgca ggtgaggctc ttgcgggtgc
     1141 ttcaggaacg ggagatcgag cgggtggggg ggacaaaccc gatcaaggtg gacgtccgcg
     1201 tcattgccgc caccaaccgg aaactggaag atctgatcag ctcgaatcgt ttcagggagg
     1261 acctctggtt tcggctcaac gtgttcccca tccggattcc gccgctcagg gaacggaaat
     1321 ccgatatccc gcgcctggtt ctgcatttta tcctgaagaa atcccgggaa ttgaaccttg
     1381 ccgtcgatcc ggtcctcgcg ccgggagcga tggagaaact catggcctac gactggcccg
     1441 gcaacgtccg ggagctggaa aatattgtgg agagggcgct gatcagaatg cgcaccgggc
     1501 acacgtacag gccgctcacc ttcgatgagt tcccgatgga tccgccgtcg gcgagaccgg
     1561 cggaaaggga attcccaagc ggaccgaaag aggccccgtg tcatctggat gacgcggtgc
     1621 ggcagcatat tcaggagatg ctcgacagga ccgggggaag ggtccaggga gccggcggcg
     1681 cggcggaacg cctcgggatc aagccgaata ccttgaggaa ccggatgcgc aagctgggga
     1741 tccccttcgg gaggaagtcc catcgcggaa cacccggcga ttgaccacga tccgcacgca
     1801 aacggaaacg gaagcgtgtc cgagaggcgg tccggagcgg attcgcatac cattttacaa
     1861 gggggaaaag ggatagcatg ttggacgtgg agaagtacgg caaacagacg gttcacgacc
     1921 tcctgctcaa gcacctgatc gtcatcaacg cgggctggtt caagttcgag aatctgatcc
     1981 gggcgacgca tcccgaggtg ctggcgggca acgggtatgt gcagatcatg gaagagctgt
     2041 gttcggacca ggccaggggg ctcgccaggg tgttgggcgt tcaaggcgat ggcatcgaca
     2101 gcatgatcga gcttcttcgc tgttcgcaat ggggcgtttt tgagcgtttc gagatcgaaa
     2161 ggctttcgga aacgagctgc ctgatgcgga tcatgggctg ttcgacgcag cgggcggccc
     2221 gaaagtgggg gatggatcac tacgcgtgcg cggaggcgac gatggtctgt ctcgggagct
     2281 tctgcaagca ggtcaaccgg gatgcgacca tccacaagat ctttgcgccc cccgaagtcg
     2341 atccggtcgg aaaccgtgga caggtttcgt gcgaatgggt gatttcgatc gacgggacgc
     2401 ccggcgtctg aacggcgcca ccgctcgccg gggggccggt tcttttgaat gcatgtcatc
     2461 aaaagggggg atatgccgat ggaacagacg gcgttcaggg tctaccgcta tcgatgggtt
     2521 gtgctcctcg tttatgttct tctcaacgcc ctgatgcagg tgcagtggat cacctttgcg
     2581 cccatcacga gcgaggcggt cgcattttat ggcgtgcccg ccatacagat cgacctcctc
     2641 tccctgattt tcatgattgt ctacatcttt gtctccctcc cggcctctta tgcgatcgac
     2701 acctggggga tccggatcgg catcggcatc ggtgccgtcc tgatgggcgt cttcggattc
     2761 atgaagggcg tctgcgggtc gagctatgcc ctggtcgccg tcgcccaggt cggcattgcc
     2821 gtcggccagc ccttcgtcct gaattccgtc accaaggtgg gtgtccgctg gttccccctg
     2881 cgcgagcgag cgacccaggc gggcatcagc gtgctggcgc agtttgtcgg catcattctg
     2941 gcgatggtct taaccccgct gctcttcaag aaatacggca tggaaaccct gctgatgatt
     3001 tacggggtcg tgacattggc gggcgccgtg atctttctga ttttcaaccg ggagcgtccg
     3061 gccacgccac cctgtccgcg gggtcaggac gagcggatcg ccgtcctggc gggattgaag
     3121 catatcttcc ggcagcggga catgatttac cttctgctcg tcttctttat cgccctgggg
     3181 atcttcaacg cggtgacgac ctggatcgaa cagatcgtcc ggccgcgcgg tttcgacatc
     3241 acgcaggcgg gggtcacggg ggcgctgatg atgtcgggcg gcattctcgg ggcggcgatc
     3301 ctgccgtatt tctccgaccg gctgcggaag cggaagatct tcatcgtcgc caccatgatc
     3361 ctcgccattc ccggcctcgc cggcctggcc ttcgccggca gctacgagct gttgctggcg
     3421 tcgagcctcg ccttcggttt cttcctgatg gcgggcgggc cgatcggcta tcagtacagc
     3481 gcggagatct gctatccggc gcccgaggcc acgtcgcagg gcctgctcct gctggcgggg
     3541 cagatttcgg gcatcgtctt tgtcatcggc atggatgtcc tggcggcggg gagcgactcc
     3601 aagacattgg ccatgatcgt gtttctcgtc ctgatggcgc tcaacgtttt cttgacgacg
     3661 aggctccggg agtccacgct cgtgaagtcc ggagggatct gagcgagata atgattgact
     3721 tcaaaagtca aaccttatta agacatgcaa agccgccgga cggcggggag ggctgcctcg
     3781 ccgcgtcggg ggatgtgagc agaaccgttc gtgtgtggat tcgagccatg ggggaggatc
     3841 atgaaacgca ctgacaagta tgttctggcc gtggatttgg gaacgtccgg ccccaagacc
     3901 gctctggtct ccgtccatgg cgacgtggtc gacagcgagt tccaggacac gcccgtgatc
     3961 ctgtttcccg acggcggcgc cgagcaggac ccgcggggct ggtgggacgc catccggtcc
     4021 accgcgaaga aggtcatcgg caagaaccgg gtgccgcccg aggacatcgc cgccgtgagc
     4081 gtcaccacgc aatggtcggg caccgttccc gtggatcggg acggccgcca cctgatgaac
     4141 ggtatcatct ggatggactg ccgcggttcc gagtccgtca aggacatcct gaagggcttt
     4201 ccgaagatcg agggctatcc gctgttcagg ctgctggtct ggcagaggct gaccggcggc
     4261 gcgcccgccc ggtccggcaa ggaccccatc tgccacatcc tgtggctcaa gcagaaccac
     4321 ccggacatct accgccggac ctacaagttt ctggaaccga aagactacat caacctgtgc
     4381 ttcacgggcc gattcgccgc cacctacgac tccatcctgc tgcactgggt gacggataac
     4441 cgcgatccgt cgcacgtcgt ttacaatgac aggttgctga agatggccgg catcgagcgg
     4501 gagaagctac ccgacctgat ccaggccatg gatatcctcg ggccggtgaa aagtgaggtc
     4561 gcccgggagt tggggctccg ggagggaacg ccggtgatcg gcgggacgcc cgacgtgcct
     4621 tctgcgggcg tgggttccgg cgcggtccgg gactatgaag gccatattta catcgggacc
     4681 tcctcgtggg tttccgcgca tgtcccgttc aagaagaccg acctcgccca caatttcggg
     4741 tccttcccca gcccgatccc gggccggtac atcatcctca cggagcagga atgcgctggc
     4801 aaatgcctga cgtggctgag ggacaacatc ctgtaccaca aggacgaact gctcgtcgag
     4861 gagagcgtcc ccgatatata caaggtgctg gacaagatcg tcgcccgcgt cccgcccggc
     4921 gccaatcgca tcctcttcac accctggctt tacggggaac ggtccccggt ggacgactgc
     4981 accatccgcg cttccttctt caacctgtcc ctggagaaca cgcgggagga catcgcccgg
     5041 gccgttttcg agggggtcgc gttcaatgcg agatggctgc tcgcgccggt ggagaagttc
     5101 atgggccggc gtttcgacta tctcaatttc atcggcggcg gcgccaactc ggacatctgg
     5161 tcgcagatct tcgccgacgt cttcgaccgg aaaatccgcc aggtcaagga cccgatctac
     5221 gccaacgctc gcggcgcagc cttcctcgca tcgatggcgt tgaagtacat gaccctggag
     5281 gacattccca ggaccctgaa aatcaaggcc gaatacacgc ccaacccgga aaaccggaaa
     5341 ttatacgacg aacggttcaa tgaattcgtc cactattaca aaacccaacg gaaggcctgt
     5401 gcacggatga acaaaaacgc ggcgggcgtg tgtgtgccgc cgttggtatc gcaatcaaac
     5461 gtcgggaggt gacgatttca tggaatattc ggaaatcatg aaacgaaagg atgcgattct
     5521 ggaaaggccg gtgcgatcga tcccgccgga caaactggcc gaggagcacc ggcgctacgc
     5581 cgagcgctgc aaacgctcca tcgaaatctt cgagaaggcc aaggggctga tccccggcgg
     5641 cgtcgagcac aatttaagcc agaacaaacc ctttcctctg gcgatggatc gcgccaaggg
     5701 ctaccgggcc tgggatgtgg acggcaacga atacatcgat tatctgatgt gcggcgcgcc
     5761 catcatgctg ggacacgggt acgaaccgct cgatgaaaag atcatcggga tcatccgcga
     5821 aaaggggccg gcgaccgggc tgacctcgga gtacgagctc ctcgcggcgg aagagatcat
     5881 ccggcacatg cccggcgtgg agatggtccg atttctccag tccggcaccg aggcggacat
     5941 ggcggcgctg cgcatcgccc gggccttcac cggcaagctc aagatcatca agatcggcgg
     6001 cagctatcac ggctggtccg atcaggtggt ctattccgtg cacatccccc gcaccggcaa
     6061 tttcgaatcc aagggcattc cgccggagtg ctacgcccac accgtggatg tggcgcccaa
     6121 cgatttcgag gcgctggagg cggcgttccg ggacaaccag gacaagggcg gggtggccgg
     6181 cgtgatggtg gagccgatcg gtggcgaatc gggcgcccat ccggttcatc cggactggaa
     6241 ccggaccatc cggaagctgt gcgaccggta cgaggcgctg atgatcttcg acgaggtcgt
     6301 gacgggcttc cgtctggaca tgggcggtgc ccagaagctg ttcggcatcc ttcccgatct
     6361 gacggtgctg ggcaagatca tcacgcacgg ctatccgtcg tcgggcgccg tggccggccg
     6421 cagggacgtc atgaaggtct gcgccggcag catgggcgaa aagatctttg tcggcgggac
     6481 gctggcggcc aaccccattt ccatggcggc ctgctattac gctctgaagt acatggagga
     6541 aaccggcgcg gtggccaagg cgacggccta cgccaaccgc ctcacccagg cgctcaacga
     6601 cctgtacgcg acgcgtcccg atctgggatt cttcgtgtac aatttcggcg ccatcctgca
     6661 ttacatgacg accgcgttct tttccgtgga cctgaacgcg ccggacgcgc tcacccagat
     6721 cttcagccgg aagcaggtcg cggacgacta tcaggtggtc acgatggcgc gcgggttgtg
     6781 cacgctggcg gggacccgca tgtacacctg catgcagcac gatgacgagg cgctgaagaa
     6841 aacgctgggg gtatgggaat acctgctttc gctgattccc agaggttgag gtgagagatt
     6901 gaggtgaaac gattgatccc gataaggagc gcaaaatgat gaaaaagaat caaggtccca
     6961 aagtcgtcaa gaagcaaatc ggcccctacg cggacaaacc catcgccctg cccgaaggca
     7021 tcgatatgga aaatcatgtc gtcgccacct actggatcca ggcggacaag gggtgggaca
     7081 tgtccatcat gggacaggtg atggcgatcg aacagaccac cggtacctgg gtcccggtcc
     7141 ccggcgaaac cccggaggtg cgcgcgggcc acgtcgccaa ggtgatcggc gtttatgagg
     7201 ccccgtatta cgagtacggg ctgcccgcgg acgtgacgga gcggcagtac atcctgcaaa
     7261 tcgcctttcc cgcggccaat gtcgaagacc agttgcccat gatgctgacg gcgacgatcg
     7321 gcaacatctc catggtgccg aacttcaaac tgctcgacct ccgcctcccc aaggccaccc
     7381 tggaccagta tcagggcccg aaattcggga cggacggctg gtggaaggcg ttggggctca
     7441 agaagggccg gccgctgctc aacaacatga tcaagccgtg cagcggctat ccgcttgaag
     7501 tcggtgcgga cctattccgg caggcggcct tgggcggctg cgatgtgatc aaggacgacg
     7561 aactgatcgc cagcatgcgg tacaacgacg cggtcaagcg ggtcaagacc tacatgaaga
     7621 tcgagcggga ggtgtacgag gaaaccggcg aacataccct ctacaccgtc aatgtcaccg
     7681 accgcctgcc gcgaatgttc gatctggcga ggcgctgcgt cgatgcgggc gtcaacggca
     7741 tgatggtgaa ctatctggcc gtcggtcctg aagccatgcg cgcgctggcg gaagacccgg
     7801 ccatcaacgt gccgatcctg gcgcacatgg atctggccgg cgcctactac atgtcgccca
     7861 accaggggat cgcctcgccg atcattctgg ggaagtttcc ccgcctgtgc ggggcggacg
     7921 ccatcgtact gcccttctcc atgggcggga aggccatgta catgcacgac cgcttcatga
     7981 acacggtgcg caatctgacg tatccgatgg gccgcctgaa acccaccatg ccgatgccat
     8041 ccggcggcat cacgcccgcg aacgtggccg atatcgtggc cgccgtgggg cgcgacatca
     8101 tgatcggttc cggcggcggc atccatgccc atccccaggg gccgagggcc ggcgcccgcg
     8161 ccctgcgaca ggcgattgat gccgcggtca agggaatcaa actggaactg tacgccaagg
     8221 aacacgagga actgggggtt gccctgggcg tctggagcaa gaaaaccgac ttcaagatct
     8281 gagcgcccat cgcatgaggg acgttgcttt tgtccgcatt tgagtgtcca tcgcatgagg
     8341 gacgctactt ttgtccgtgc ggggagcggt tgcacgccgc tctccgtttt ttgaaggaaa
     8401 cccgacgttg gatgattacc tgcatgacga tccgcgcctt cccgcggcct gggcgccctg
     8461 gttctcccat gaataccttt cgtacttctg cgtcagggcc ctggacggcc tgatggctct
     8521 gccgccggtc cccgtccggc ccaatccccg tttcggcgac aggatttggc ccatcggccc
     8581 gaggcacatc gacgtttaca aggaaagtta ccgggcgcac cggcaactgg atccgctgtg
     8641 gtccttcgat tacaagaagc gaaacggcga ttttgccctc gaaccgcccg cgggaaagat
     8701 gatcgagccg tggaaggtgc tggtgatcta cagtacggag cccgatctgt atccggacta
     8761 cgatctgatc ctgcatcgaa accagaaaat caccggtggt tcccacggct ggcggcacat
     8821 gcgcttccgg cttctcggga tgaccttcgg catcgcgccc cggtccttca gcgttcacag
     8881 ggatctggcg aggctcgcgt tcgaaaacaa ccacgactat tggggatggc ggtatctttc
     8941 ccggggcgcg cactacctgg cggacctggg gcatcccttt cacgtcaagg cgctgcccgg
     9001 ctcatttctg ctgaagaaga tactggccag gcacgagctt ttcaggatcg tttccgccgt
     9061 gcatcagagt tacgaggtct ataccgaacg aaggttccgg gaggggttcg aacccttcaa
     9121 agaggctctg ttgcgcggtg cgcgtgaagg gcgggatacc ccgggggatg tgcgggatct
     9181 attgccggcc tacatcaaac gggcggagcg gaggcataac ccgattttct atttcttctt
     9241 cgaccggttc ggcccggaac tgatcgaggc gttcagccgg atgaatcccg acagccctct
     9301 ggacgcggca acgcagacca accggtgctc cgcggacgcg gcccgggtga ttttcaagga
     9361 tacccatcgg gacaagctcg attttctcga caggatcacc gtcgagattc ttgccgacgt
     9421 cggcaggatg ctgggaatgc tcttcgccgc gttctcgccg aaatcctgaa ggcggggtgt
     9481 cacccgccct aggcggggag gatcctgccc gcccggatcg acccgtcgac cttcccctga
     9541 ttcaacaccc gcctgccgtt gatgaaaacc gccgcgatgc ccgtcggggc ctggctggtt
     9601 tccgtaagcg tgttgttgtc gcggatcctg ttccagtcga tcacggtgat gtccgccgcc
     9661 agcccttttc tcaaaaagcc gcgatccttg atcccgtagc gctcggcagt cgcgcccgac
     9721 atcttgcgga ccgcttcctc cagggggatg agccgtttct cgcgcgccaa ctggaagaac
     9781 cgggggaagt ttccgaacat ggccgggttc tgcacgccct ggggtgcggg gagcgcgtcc
     9841 gtcatgaaga gggcggcggg ggaggccaac aaggcttcga cgacgggcag attgctgtag
     9901 ccgtgattga ggatgttggc gagacccttg cttttgcgtg caaaatccat ggtgttttca
     9961 aaccagtcca ttccccgctt cttcgagatg tcggcgagga acaggccgtt gaaccggttg
    10021 agttccgggt ggttcgcgta cgtgatctga atgtctttga ccccgaagcc caggaaccgc
    10081 atggtctgtt gccattcgtc cctgagccgg gcgagcatct tggggtcgtc gtacgcctgc
    10141 gggacttttg ccaggaacca ggccgggaag atgacattga tcacggactg tccgcagtgg
    10201 tacgcgtagg tatcgaattt gacatcgacc ccccgccgga tggcccggtc gatgcgcttg
    10261 agggcgtctt cgcaggtctt gaaggtccgg gttcccacga aaatgagatg ggagatctgc
    10321 aggcgcacgc ccgtttcgcg agcgatgttc agcatctcgt cgatggcgat caaattgtgg
    10381 ggttcatagc cgtcgaaggg catgatgtag tcgagcagga tcttgggcac gctcatcggg
    10441 tatcccgggg cgagggccga atacgcccga agatggacgg tcaggatctt gcccttcctc
    10501 ttgacgagcc tggcgatctc cctgagttcg gcggtctggg cgaaaatgcc cggctcatac
    10561 tgaagcccca gcgagacgcc gaccgcgcct tcgtccagcg actgctccag aagcgcgagc
    10621 atctccttca tttcgtccct gttcatcggc gcggcgctga atccgcggat cgaggtccgg
    10681 gtcgtgccgt gtccggccag gttgacgagg ttgtgggtca ggccggtctt ttcgatgtaa
    10741 tcgaagtact gggccatggt atcccactcg atgcggaaga gccccttggt cctgggtgcg
    10801 atcaggttca tgtggggcga gtttttccga aacccggcaa cgccgtatcc gcaatggcct
    10861 gccacgaagg tggtgacgcc ctgggccgtg aaggggagct tgagatcgtc acgcccccgg
    10921 atgggcagat accagtccat gtgggagtgg gcgtcgatga ttcccggcat gatcacctgc
    10981 cccgtgcagt cgacgaccgg gcaggacgcg cggatctcgt cccgggagat cctctcgatc
    11041 ttgtcgtcgc ggatgagcag gtggcccttg aatcctctct ttcccgagcc gtcgacgatg
    11101 aagccgttcc tcagaagcgt atcatggccc cccgtgcccg tcaccacccc cctgccgggg
    11161 atcttgccgg cggtcggttt ggcgtttgcc gttcctctca gcagccccag acccggcaac
    11221 gccgccagca gggccatcga ttttccggtg aaggccagga agcgccttcg ggagtgtttc
    11281 gtcttgatca gccgttccag acttgacttg ccgttcatcg catcgcctcc tcggaaaggg
    11341 ttttgtcacc gactgaacac cgtcgaaagc ggggcgggca aagccacccc ccgccaggct
    11401 tcacttccgc gctttgatcc cacagggcgg agagggggac ggaccgtcgc cccgtgcccg
    11461 aaaatctacc ccggtcgccc gggtgagtca aggggcattc tgcggggacc ccgggcggtg
    11521 cttcgttgaa aaatatgtct ggaattgggc tgccggcgat gatacaaggt gccgcttccg
    11581 aaagcgcgga ttcacgaaaa ccgggcgcga gaaatcgcga gaggagaggc ggacatgtac
    11641 agaaagttgc tcagcccgcg gatatccgag atcaacggag cgggacacgt cggacacaac
    11701 gtcatccccg tctggctgga ggagggattt ttggagatca tccggctgtt caatcccgag
    11761 ctgcggggga agcccggaga ccccttcctg gtcatggcca acgtcaacat cgacttcgtg
    11821 aacgagatct tcctgggcga agacgtcgag atcgtcaccg gcgtcaagaa gatcggaaat
    11881 gccagcttcg tcctgattca gcagatatac cagagcggga aattgtgcgc gaggggaagg
    11941 ccgacctttg tttacctcga tccgcagtcc cggaaatcca ttcccatccc gccgcccatt
    12001 cgtcgcgtcc tggagaagca cgtcgtggag gcctgagccg gctcctcccc ggccccgcta
    12061 cgcttcccgg atcgctgcat ttcctggccc gcggcaacgt caagcatcgt tcgccaggcg
    12121 cgggaggaag gggggcgggc aacgtcaagg gttgtgtatc aaggatgagc ggaaggggtc
    12181 tccccgttgc cgcctgtgtt tccgttgaca cctccggcgc cttcccctat actccctcga
    12241 aatccagcgg gaggcggggg aggggtcgtg acgtccgcga gaatcaaggg gcatgggatc
    12301 gccggaggcc tgtttgtcct gctttttctg gcgctgctcg ccgttcgctt ggacgtcttt
    12361 cccggggccc tactccccgg gtccggaccg gccgcgtctt ccgccggccc ctcccgggct
    12421 gatgaagacc gatggatggc cgtctaccag agtgggcgga aaatcggcta ttcgcaccgt
    12481 cggatgcagc gcgtcccccg ggggtattgt ttcgaggagt ccggcctgct gcggatcaat
    12541 acgatggggg tcgttcagga catccgcatg aaaacgacgg gggatctgaa tccggacggg
    12601 accctgtccg ccttccgctt cgatcttcaa tccagtctct tcagcttcgt cgtgcgcggg
    12661 gaggtccggg ggcgtgacgc catcgtttat caagggccgc cgtcggcgga gaagcaaatc
    12721 cggatcgcgc tccgggagcc cccccacctg tccaacagcc tttacgaggc gctccggggc
    12781 aaggcgctcg caaccggcca ggagatgacc ctgaacgtct tcgatccggc cttgatggcc
    12841 gagcgcccgg tacggatcac cgtcctggag cgtgaaacga tccaaatcat gggacggccg
    12901 cacgcggccc gcaaggtcga ggtggatttc atgggcgccc ggcaggcggc gtggctgggg
    12961 gaagacggcg gggtcctgag ggaacagggc ctgctgggga tgaccgtcga acaggtcacg
    13021 aaagaccggg cgctggacgg gttgacgctg gccgccagcg ccgatctgac cgacgccgcg
    13081 tcgatcccct cgcgggtgcg cctcgacgcg ccggagaccc tggcgcacat gaccgtccgc
    13141 ctggcaaacg tccggcccga atcgctgttg ctcgacggcg gccgacagcg gtatgaacag
    13201 ggccggctca cggtcgtgcg ggaggcgatg cccgcggccc ggacgacccg ggacgcgctc
    13261 ccggcggaaa tgcgtccgtt cctgcaggcg acgcccttca tccagtcgga tcatccggag
    13321 attcgcgccc gggcggcccg gatcgtctcc cccggggacg gcgatgcggt caaggcccgg
    13381 aagctcgtga actgggtgta ccggaacgtg gagaagcggc cggtgctgtc cgttccgaat
    13441 gccctggaaa ccctgaccca tcgcgtgggg gactgcaacg agcacgccgt gctcctggcc
    13501 gcgctcgccc gggcggcggg catcccggcg caaatcgagg cgggcctggt gtatctgagg
    13561 gggcggttct attaccacgc ctggaatgtc ctgtacgtcg acgggtgggt gacggccgac
    13621 gcgacgatgg ggcagatgcc ggcggatgtc acccacatcc gtctcgtccg gggggaggcc
    13681 gaccgccaga tcgatctggc ggggatcatc ggggacgtcg acctggagat cgtggatacg
    13741 aaaaaatgat cgaactgacc gacttgacca aacgttacgg gaaaacgctg gccgtggacc
    13801 ggctgaacct atccgttgcc cccggggaga tcttcgggtt catcggaccg aatggggccg
    13861 gcaagaccac gacgattcgc atgatggggg gaacgctctc cccgacggcg ggtgccgtcg
    13921 tcatcgacgg gatcgacatg gcggcccggc cggaggaggc gaaacggcgg atcggcttca
    13981 ttcccgaccg gcctttcctc tacgaaaagc tgaccgggat ggagtttatg cggttttccg
    14041 cggacctgta cggggtgggc gacggaacct tccttcaaaa ggccgaggag ctgctccgga
    14101 cattcggcct gttcgactgg tcgcacgacc tgatcgaggc ctattcccac ggcatgaagc
    14161 agcgcctggt tatctcggcg gccctgctcc acaatcccag ggtgatcgtg gtggacgagc
    14221 cgatggtcgg cctcgatccg gccgccatca agatggtcaa ggacatcctc aaggatctgg
    14281 cccggcgggg aaccgccgtc ttcatgtcca cccacaccct gagcgtcgcc gaggacctct
    14341 gcgaccggat cggcatcatc catcggggcg cgctgatcgc cctgggaacg acccgggagc
    14401 tgaagcgact ggcccaggcg cagcaggccg atctggaaga ggtttttctc aagctgacgg
    14461 aggagcaggc cctttcatga acggcgtctt gaccttgctg aaaccccggc tctggtcctt
    14521 tctgaacggg aatgtcgcgc gggaccggga accccggaac atcaaatggc tgctcttcgg
    14581 cgggatcggt ttcctgttct gggcgggcat cttcaccgtc ttctaccgtg tcctcagata
    14641 cttccagggg gtggagggct tcggcgacat cctggcgtac aagctcctgt ccatggtgct
    14701 catcacgttc ttcgccctgc tcattttcag cagcatcatc accgccctgt ccaagttcta
    14761 cctgagccgc gacctcgtcc tggttcacgc cctgcccgtg tcgccggcgc agatctttct
    14821 ggcccgctgg atcgaaagca cgatcgacag cacctggatg gtcgtcgtct acagcctgcc
    14881 gatctttctt tcctacggca tcgtttaccg ggggggcatt cttttttatg ccacgatcct
    14941 gggcgccctc ctgccgttct gtctcatcgg gtccgccctg agcgccatcg cggtggtcct
    15001 ggtcgcccgg tttctcccgg cggggcgcat tcgcacggtc ttcgtctttc tcagcctggt
    15061 cctgttcgtc atcctgctga tcgccttccg gatgatgcgg ccggaacagc tgaccaatcc
    15121 ggacaccttc gcctccgtcc tgctgtattt caggtccctg gagacacccg ggtcgccctg
    15181 gcttccctcc acctggatct atgacagcct ccgggaagtg ctttccggga accgttcggg
    15241 agcgctcttc cacctggcgc tgctctggag cggcgcggcg gcgctggggt atctcatgac
    15301 gtggatcgcc ggagcggctt acttccaggg tttctcgaag gcgcagacca cggccgaacg
    15361 gctgctcccg tcgcggcagg ggcggggggc ggcgggcgcc tggctgctgg gctttccgtc
    15421 cggcccaacc cgggcgttcg ccgtcaagga aatcaagacg ttcacccggg atcagacgca
    15481 gtggccgcag atcttcctga tgatcgccct gatcgtggtg tatctctaca atttttccgt
    15541 tctgccgctg gacaagagcc ccatcaagac gttttatctc cagaacctgt tttccttcct
    15601 gaacatgggg ctggccgcct ttgtcctgac ggccctgtcg gcccgcttca tctttccggc
    15661 ggtgagcacg gagggcgtcg ccttctggat cgtccgggcc tcgcccgtgt cgatccggtc
    15721 gtttctgtgg gtcaagtttt gggtgtattt tctcccgctc gcgatcctgg cggaatttct
    15781 gatcgtcgcg acgaacctgc tgctgcaggt cacggacctg atgatgatcc tgtcgacggc
    15841 gacggttctg ctgatggtgc ccggcatcgt ggccatgggc gtgggactgg gcgccgccta
    15901 tcccgatttt cagtcggaga atccggccca ggcggtcacc agtttcggag ggctgctctt
    15961 catgatcctc tgtgccgggt tcatcgggtt catcgtcatc ctggagaccg gaccggtcta
    16021 cagcgtcttc atggcgtcgc tcaacggccg gggcctcgcc ctcctgcagt gggtctggct
    16081 ggcgatctct tttgcgctgg tgatcctgct ctgcgtcctg gccgtggtcc tgcccatgcg
    16141 cttaggcgag cgccgtctga aggcgctcgc ctaagcggcg cgggaagacc gtcactcttg
    16201 atacccgggg tggcggaagt ccgaccgcgg atggcgccgc gcgtccccgc tcagggtttt
    16261 cccatcgcgc ggttcaggat gcgctgaaaa tcagcgcggc agaaatcgcg gggagcgagc
    16321 gggcggaggc gataggctag ggcgtcctcg gtgatgtcct tgagcgcccg gtcacggacg
    16381 ccgacgtccg tcaggcagcg gggaatatcc aggtcggcct tgaggatgtt cacggcgtcc
    16441 acggcggcga aggccgcgtc gcgcaggctt aggcccgaga tgttttcgcc cagcgcagca
    16501 gcgatgtccc gaaagcgagg catgtccgcg atgatgctgg cttccatgac atagggcagc
    16561 atcagggcgt tagccacgcc gtgaggaacg tcgaagcggg cgccgatggc gtgcgcgagg
    16621 ccgtgaacgg agcccaggcc ggcgaaggtg aagccgaccc ccgccaacgt gctggccgcc
    16681 gccatacccg agcgcgccgc gaggtccgtc ccgttgcccg tggcccggcg gaggttttcc
    16741 ccgatcaggc ggatggcctg gaggcagaag gcgtcgctga agggttgatg gctttttgat
    16801 gtatatgcct cgatggcgtg ggtcagggca tccatgcccg tcgcggcggt tacggtgggc
    16861 ggcatgctca catgggtttc cgggtcgatg acggccgtct tggcgaaggc gagggggtgg
    16921 gagaggccgg tcttctgttt ccgctcgtcg tcccggatca ccatggccgc cgtcacctcg
    16981 gagccggacc ccgccgtcgt cggaatggcg aggaagggaa ggccggggag cttgaaaacc
    17041 ttcttgacgt acaggtagtc ctcgacggcg cccttgttcg tcgcgaacac ggcgatggcc
    17101 ttgccggcgt ccatcgggct tcctccgccg taggcgacga tcaggtccgc gccgctcttc
    17161 agggcggtct gccgggctgt ctcgatggct tcgagcgtcg ggtcgtgctc gatccggtca
    17221 tagacctcga acgggagatc cgcctcccgc atcgactcga gcgcggcggc gaaaccgggc
    17281 gacgtcgcgg tggccgtcgt cccggtgatg acgaacgcct tcttgcctcc gagcatccgg
    17341 cactcctgcc cgacctgccg aaccttgccg catccgaaca ccacccgagt gggaagaaaa
    17401 aacgtaaaat cctgcattgc cagcctcctg agtttggtgc ggtccgcctc gggaaggtcc
    17461 tgcatccttc ccggagcatg cgcagattag cataggacag ggacgattgt caccgagaaa
    17521 tggcggggga gggagcggcc gcgtcagagc ggaccccagc ggccggtccg ttcagtccgg
    17581 tcccccacgg tgagggtcag gacattgccc ggcgaagcgg gatcaaccgc gacctccacg
    17641 ccggccagat cgaagtcctt ctgcaaggct ctaagctggg ctttcttccg gcccttcaag
    17701 tgggccgcca gggtcggggc ggcggtgaag aggattgtcc ggtggcggac ggggagccca
    17761 gccagcaggc gggccgccat ctctccggcg atggattctt cgacccggct ccggagagcg
    17821 gggtgatacg gtccggcgag aaccgccccg ggcgcctcca tctcgggggt cgtctgcacg
    17881 ccgaggcgga tcacggggat gccggcgttt tcgaaggccc cgagggcatt ccggcaggcc
    17941 gcaagggtgt tttccaggga caggggcgcg taggcgccgg cgcggtagag gtccgccagt
    18001 tcggtccccc ggaagaccag cgtggggtgc aatcgaacca tgtccggctg cagggcgacg
    18061 gcccgccgga cggtctcggc gaacgcagcc ccgtcctctc ccggcaggcc gatcatcagg
    18121 tgcaaccccg tggcgaagcc tttcgtcttg aggcgcgcga cggtccggac cacgtccgcc
    18181 accgtgtgtc ccctccggct cttttccagg acctcatcga ccagggactg ggccccgatt
    18241 tcgaccgtcc acaccccggc attccgcagg aactgggcgg tgtcgtcatc gatttcatcg
    18301 gggcgggtgg agatccggat gccctgcacg aggccttccc ggagacaggc ggtggcgg
//
LOCUS       DAOOLJ010000034        16489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3695_length_16489_cov_18.093952, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000034 DAOOLJ010000000
VERSION     DAOOLJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 16489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16489
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_16489_cov_18.093952"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="meaB"
                     /locus_tag="PLW16_06770"
     CDS             complement(<1..929)
                     /gene="meaB"
                     /locus_tag="PLW16_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB70644.1"
                     /translation="MDKASKIRAGDVRTASRLIRNLEDQLPEATETLKHIFPHTGKAH
                     IVGITGAPGAGKSTLTDALISSFRHQNRTVGVLAVDPTSPFTGGAILGDRIRMQRHAE
                     DPGVFVRSLATRGALGGLSKAVGDAVHILDAMGKDIIMVETVGTGQQEVEIINHSHTV
                     VVVLVPGMGDEIQAIKAGILEIADIFIVNKADRDGARQLHRELQMMLDMANLKPGAWR
                     PPIVMVENVFQPEAFTGQMEEASRQIDAHYRYLIESERMEDRMRRQVLVEINEAIRAS
                     ILEPVLKDLVASGELDRMMSALFKKESDPYSLA"
     gene            complement(974..1375)
                     /locus_tag="PLW16_06775"
     CDS             complement(974..1375)
                     /locus_tag="PLW16_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPB70645.1"
                     /translation="MSRKIRIMVAKPGLDGHDRGARVLARCFRDAGFEVIYTGCHQTP
                     EQIAAAAVQEDVDLVGLSCLSGAHRYLFPRVVELLREQGASDITVIGGGIIPEQDFSL
                     LKDAGLKAIFTPGATLEEIVAWVRENVGPQG"
     gene            complement(1582..3264)
                     /locus_tag="PLW16_06780"
     CDS             complement(1582..3264)
                     /locus_tag="PLW16_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB70646.1"
                     /translation="MLHAKETLEQSARLTRQWEDEVRRSVAENPDQKARWSTVSDLEI
                     KRLYTPEDIQAMDFARDIGVPGEYPFLRGNQATGYRGRYWTYRMFSGMGSAQDTNERW
                     HLLLKSGQTGLSTAFDFPTLMGYDSDSPKARGEVGKCGVAIDTLEDFLVLIDGIPLDR
                     VTTSMTINPPATILWAMYCAAAEVKGVSLDRIGGTIQNDMLKEFIAQKTFMCPPEPSV
                     KLISDTIEFGTKHVPKWNTISISGYHIREAGSTAVQELAFTLRDGIEYVEDVIRRKGL
                     DVDAFAPRLSFFFNAHIDFFEEICKMRAARRIWARVMRERFGAKNPRSWWMRFHTQTA
                     GCSLTAQQPYNNVVRTAVEALAAVLGGTQSLHTNSLDEVLCLPSDHAVEIALRTQQIL
                     AEETGVANTIDPLGGSYFVEALTNEMEAKALEYIAKIDEMGGMTAAIDKGYPQMEIAD
                     AAYHFQQQLESGEKAMVGINRYATGEAQEIPYLEIDEAVEKQQVARLAAVRRKRDGNA
                     VRRSLDDVRRACRQGDNVMPYCIEAVKNLASVQEICDVYREVYGEYRDPGLF"
     gene            complement(3708..4475)
                     /locus_tag="PLW16_06785"
     CDS             complement(3708..4475)
                     /locus_tag="PLW16_06785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70647.1"
                     /translation="MFKDVIVLITGAASGIGFGTARLFISEGATVVGFDLEAAALART
                     RDDLGERFVSRPGDVSNEADIAAAARFVEERFGRLDTLVNNAGRGTLVQMEAMQESDF
                     YWHYDVLVKGPMLMVKHFAPLLRKSANPSILNISSSAARVEHTQNHFLYSTAKAAMLK
                     FTQHIVRDLPGIRANTILPGWIDTPIYARAGLDEATIKLVYEHALPRIPAGRIGSPED
                     IGHCILFLSSPKASYINGASIDIHGGYLSNADWGFLW"
     gene            complement(4503..5693)
                     /locus_tag="PLW16_06790"
     CDS             complement(4503..5693)
                     /locus_tag="PLW16_06790"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB70648.1"
                     /translation="MARGKVSKADVGHARKLGLDPDRLLRIREVIEADTTQGLYDGAV
                     FIVARHGEIVLHEAVGHTDLAKKRAARKDDVFFIMSLTKQMTTVRVLMDIEAGKFTLT
                     TPIREILPEFGIKGKQNITVGHVLTHRTGLNTEIPFTLPVDKLGNIEAVVACLSNERV
                     LFMPGTIVTYNPTTAHALVAAMVQRLDAKKRSFRRILAEDVFAPLGMKDTALGLPDRL
                     KKRLVPVVVRDKTRGIFDPDLIEALNYIAAEDTEMPSGGGVSTAHDVFLFAEMLRRGG
                     ELNGRRLLSPETVILATTNQTGDQPNHLMDYMREMYGWPEIPAFLGLTFFLRGEGVFP
                     TPLGINTSPDTFAGLGAGSTMFWVDPERDLTYVFLSAGLMEEGKSFLRHQRLSDMVVA
                     AVVD"
     gene            complement(5835..6806)
                     /locus_tag="PLW16_06795"
     CDS             complement(5835..6806)
                     /locus_tag="PLW16_06795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70649.1"
                     /translation="MVTDGSPSVRSWFLTLQAKSQQKGGSMGQERVALITGCGSGIAR
                     HLTTVLSGRGYRILATDINPESLEHSARELKWNADRVKLHTLDVRDAEAWKEAVAVVV
                     RTWGKLDVCMNIAGFAIQGFVHQFPLEHIDLHIDINTKGAILGTRFAAEQMIKQGSGH
                     IVNMCSLAGLAPVPGTSLYSASKFALRGFSLAVSYELKPRGIDVSLIEPDLVDTAKLQ
                     TQLIYGDEAAAVGFSAPRILSVEDVVAVILKRVLEEKVLEAAIPPWRGWLCKFGGTFP
                     RLTGQIHKIVTKMGMKNLSREKAKRQGYAQVVTPDKWLYEREVKKKK"
     gene            complement(6847..7485)
                     /locus_tag="PLW16_06800"
     CDS             complement(6847..7485)
                     /locus_tag="PLW16_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB70650.1"
                     /translation="MQAAVAKRRQSERMERRERILGAAKTVFLKKGYFKTNIRDIAKA
                     AALSPGAIYYYFGGIDEIYAEICEASFQMINRFLERECGEKASPLKKLENMADAFSRF
                     YLEHPEYFDLFAFSDLGWKRVGLKEELASRLDQALTRAISLVQSVIEEGIRKGEMKPV
                     GDRRQIAYALWSGIEGALSINRRGLLENTDFGIAALIQTQMRMILRGLAGSP"
     gene            7825..>8950
                     /locus_tag="PLW16_06805"
     CDS             7825..>8950
                     /locus_tag="PLW16_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB70651.1"
                     /translation="MAESFPVVEEIRALCRPRSVAVVGVPRGLKTGRLFLMALQDQGF
                     PGPIYPINPRIDEIDGLKVYPSVAAVPGEIDLAIVLIPHDQVMDCIHDCVAKHVRGAV
                     LFTAGYKETGTAEGAALEAEIVRIARAGGMRLIGPNGMGLYRPAAGLSFFPQLPRETG
                     PVGLISHSGSLTNIIGRMGAERGLRFSTVVSLGNECDLSAGDFLAYLGEDPETEVIGG
                     YIEGIRDGTRFLAALKHASRRKPVILWKVGLTPEGAKAAASHTGALAGSEEVWRGVAR
                     QGGAIPVAGFEAWVDALMGFALIRPPRGRRIAILAGPGGLGVSAAEACGRAGLTLAAI
                     APETRERLARFVPATGTSLGNPVDVGMSASLDVELYIEAAR"
     assembly_gap    8951..9050
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9051..9323
                     /locus_tag="PLW16_06810"
     CDS             <9051..9323
                     /locus_tag="PLW16_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70652.1"
                     /translation="TFVSFILFLIPGFPKDSLCYIIGLSHMKTSTFVVIATFGRLFGT
                     LGLSVGGNFARGGQGLLLLLALGGVGLVLLLAYLFRDRWLRLKKKG"
     gene            complement(9330..10730)
                     /locus_tag="PLW16_06815"
     CDS             complement(9330..10730)
                     /locus_tag="PLW16_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70653.1"
                     /translation="MRDEAKTKRQLIEELCALRRGRAEDAARPSRPAIARKAPVGIQG
                     NIPDISERKRMEEALRESEERFRTLFLQAPIGIFIYDRALIVTDCNERFARIVRSSRD
                     RIVGLDLASLREPAVIPHLLVALDNRPSHYEGRYRAMTSDADIWVSFNVNPLHNAERE
                     VVGGIGAVEDVTDRVKMASALRVGEERYRQMVEKANDIILRCDRRGCFTFVNPATERI
                     TGYAASDLLGRSYLDFIPPDHRGDVTAMFTEQFAEKTMNTYSELPIVAKDGRTMWFGQ
                     NSQLILDGDRIEGWQVVSRDITDRKRMEEELRRLSISDQLTGLYNRRGFMTLAEQQLK
                     LAVRVRQGFLLYFADMDNLKAVNDRFGHYEGDRALIQTAAILKEVFRASDIVARIGGD
                     EFAVLAVSSHLRDAEILKLRLNRRIEAVNAEGRNRFEIALSLGVALFDPAQPRTLDDL
                     MSEADELMYAQKNTRK"
     gene            complement(10845..13325)
                     /locus_tag="PLW16_06820"
     CDS             complement(10845..13325)
                     /locus_tag="PLW16_06820"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPB70654.1"
                     /translation="MATSAGTRGKAGIRTGGSVARIKQAILDNLVCIQGRFAPIATPN
                     DYYLAVAYTVRDRLLAQWAKTAHTYYERASRTVCYLSAEFLLGPHLENHLINLGLYDE
                     VRRAVAALGLDFQALVDQEEEPGLGNGGLGRLAACYLDSLAMLNIPAIGYGIRYEFGI
                     FTQEIRDGWQEEVADQWLRKGNPWEVPRPEIAYDIPFGGRTERVRDAAGRDRIRWIPD
                     RLIRGVAYDTMITGYASPSVIMLRLFKAEATTSFDLRSFNVGDYYGAVHDKIGSENLT
                     KVLYPNDEPVAGRRLRLMQQFLFVSCALRDMIRIFLQRETDLTRFHEKYAIQLNDTHP
                     AIGVAELMRLLVDIHGLGWEEAWEITRRSFGYTNHTLLAEALETWPVPLFAGVLPRHL
                     EIVFEINRRFLDGIRRDYPGDEDLARRLSLIDESGERYVRMAHLACVGSHRINGVARL
                     HTELLRAQVLNDFNRIGPEKFLNVTNGVTPRRFVALANPALAALITEAIGPAWIRDLA
                     RLEALEPFAEDAAFRVRWRTINRENKARLAELVESRTGIATDPDSLFDVQVKRIHEYK
                     RQHLNVLHVITLYNRIRRGEVGDRPPRTVLFGGKAAPGYAMAKLIIKLVHAVAETIHS
                     DSKAAGRLKVAFLPNFNVKTAHLIYPAADLSEQISTAGKEASGTGNMKFSMNGALTIG
                     TLDGANVEIREEVGAENFFLFGLTAEEVERSWREGWQPDGAIAANDDLSEALEMLEAG
                     FFSRNDRELFRPLTRTIRDRDDYRLCADYPSYIEAQDRVGRTWLDQDAWQRMSILNVA
                     RMGKFSSDRAIREYCEKIWRVQPVEIDD"
     gene            13620..13823
                     /locus_tag="PLW16_06825"
     CDS             13620..13823
                     /locus_tag="PLW16_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB70655.1"
                     /translation="MPLYDFECEKCHHTFEAILRMDEPWDTLACPSCGRKKVRKKVMP
                     IRTNAWSSFLDGMEKRVNPHKFK"
     gene            13929..15500
                     /gene="hflX"
                     /locus_tag="PLW16_06830"
     CDS             13929..15500
                     /gene="hflX"
                     /locus_tag="PLW16_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPB70656.1"
                     /translation="MTHELARELTELSRELNRQIGLLVSRQGEVAGVIVGTHQQIVIP
                     ALDSFRAASTRFKGLRLIHTHLNAEGLTHDDLTDLALLRLDLICAVVAAADGLPGMAH
                     AAHLIPENPAGEFWQVLPPARPSELDLDFLAFIQALEGEFQKQQRTRRVEAADRALLV
                     RAETDPNGEPEACLDELRELAHSCGVEVFDAVLQYRDRMDPRFVIGRGKLADVVIRAL
                     QIGANLLIFDHELTAAQARSISDFTELKVIDRTQVILDIFAQRAHSREGKIQVELAQL
                     KYRLPRLVKMDAALSRLTGGIGARGPGEMKLEIDRRRVRERIHRLEQDLKGIGKARNQ
                     RRVRRERTGLPVVSIVGYTNAGKSTLLNTLTKSRVLTEDRLFATLDPKSSRLRFPRDT
                     EAVITDTVGFIRDLPPDLFAAFRATLDELKEADCLLHVVDASNPRFENQIAAVEKILA
                     DLSMTGKPTLMVLNKADRVADKDRLAALSRRYGTVAVSALDPGTLPGLLEALERLIGR
                     LSPPGVGAMIESAAG"
     gene            15533..16096
                     /locus_tag="PLW16_06835"
     CDS             15533..16096
                     /locus_tag="PLW16_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70657.1"
                     /translation="MPEIAYTPLGRNALFTGLIGHGAALLVGIMPAAGGERFFGIVFP
                     LACLALLLGGYAELKRGGRCPLSDWRFYGIAAATVVPVLGPFVVLGLLFGFQNGVQGE
                     RIGPLGLFPALVRLRANGLVLFLWIVFLVVLFAFTGSRDDPYFKRHRDPSVRNAPQSL
                     SDACRYGLSGRAPQPVGLDGKACRQSV"
     gene            16116..16475
                     /locus_tag="PLW16_06840"
     CDS             16116..16475
                     /locus_tag="PLW16_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70658.1"
                     /translation="MKKLLLAILWSQVIFSVAADPAGAGQVTVHNRHDRDWDVGVVFT
                     QSAAEDIRSVIVEPGRTETVVNWYWGVGAINAQELRADPNHLFRRCEYAVTGMVWPGR
                     GFTLTLGSDGTCTFTEP"
BASE COUNT         2739 a         5467 c         5160 g         3023 t
ORIGIN      
        1 tcggccaggg agtaggggtc ggactccttc ttgaagagcg cggacatcat ccggtccagt
       61 tcgccgctcg ccacgagatc cttcagcacc ggctccagga tgctcgcccg gatcgcctcg
      121 ttgatctcca ccaggacctg gcggcgcatc cggtcctcca tccgttcgct ctcgatgagg
      181 taccggtagt gcgcgtcgat ctggcgggac gcctcctcca tctgcccggt gaacgcctcc
      241 ggctggaaga cgttctccac catgacgatc ggggggcgcc atgcgccggg tttcagattc
      301 gccatgtcga gcatcatctg cagctcccgg tggagctgac gcgccccgtc tcggtccgcc
      361 ttgttgacga tgaagatgtc ggcgatctcc aggatcccgg ccttgatggc ctggatctcg
      421 tcgcccatgc ccggcaccag gacgacgacg accgtgtggg aatggttgat gatctcgacc
      481 tcctgctggc ccgtgccgac ggtctcgacc atgatgatgt ccttgcccat ggcgtccagg
      541 atgtgtacgg cgtcgcccac ggccttcgag agaccgccca gggcgccgcg cgtggcgagg
      601 ctgcggacga agacgccggg atcctcggcg tggcgctgca tgcggatgcg gtcgcccagg
      661 atggcgccgc cggtgaaggg gctcgtcggg tcgacggcca ggacgccgac ggttcgattc
      721 tgatggcgga aggaggagat gagggcgtcg gtcagggtgc tcttgcccgc cccgggggcg
      781 ccggtgatcc cgacgatgtg cgccttgccc gtgtggggga agatgtgctt cagggtctcc
      841 gtcgcctcgg ggagctgatc ctccaggttc cggatcagcc gcgacgccgt acggacgtcc
      901 ccggccctga ttttcgatgc cttgtccata tcgttatccc cgtgcgccgc cggtcgatcc
      961 gcgccggacc gcgtcaaccc tggggcccca cgttttcccg gacccaggcg acgatctcct
     1021 ccagggtggc gcccggggtg aagatcgcct tcaggcccgc gtccttgagc agcgaaaagt
     1081 cctgctccgg aatgatgccg ccgccgatca ccgtgatgtc cgacgccccc tgctcccgga
     1141 gcagctccac cacccggggg aagagatagc ggtgggcccc ggacaggcag ctcaatccga
     1201 ccagatcgac gtcctcctgg acggccgccg cggcgatctg ttccggcgtc tggtggcacc
     1261 cggtgtagat gacctcgaag ccggcatccc ggaaacaccg ggccaggacg cgggcccccc
     1321 ggtcatggcc gtcgagcccc ggcttggcca ccatgatgcg tatctttctc gacatagctt
     1381 acctcgatcg tttctcttta aaacccatcg ccgcccatcg ggaatcggga tccgtcggcg
     1441 cgccggaccc tgttcgaccc tgcgccgacg ggaaaaaccc gcgctgaccg ttttcccggc
     1501 gggcgctccg ccgcgggggc atcccccccc aaaaaaaatc tcccctgagc gcaggcggga
     1561 tctggtttcc cggggccgcg cctaaaacaa ccccgggtcg cggtactcgc cgtacacctc
     1621 gcggtagacg tcgcagatct cctgcaccga cgccaggttc ttcaccgcct cgatgcagta
     1681 gggcatgacg ttgtccccct gccggcaggc ccggcggacg tcgtcgaggc tccggcggac
     1741 ggcattcccg tcgcgcttcc ggcgtacggc ggccaggcgc gccacctgct gcttttccac
     1801 cgcctcgtcg atctcgagat aggggatctc ctgggcctcg cccgtcgcgt agcggttgat
     1861 gccgaccatc gccttctcgc cggactccag ctgctgctgg aaatgatagg ccgcgtcggc
     1921 gatctccatc tgcgggtacc ccttgtcgat ggccgcggtc atcccgccca tctcgtcgat
     1981 cttggcgatg tactccagcg ccttggcctc catctcgttg gtcagggcct cgacgaagta
     2041 cgacccgccc agggggtcga tcgtgttggc cacgcccgtc tcctccgcga ggatctgctg
     2101 ggtgcggagc gcgatctcca cggcgtggtc cgagggcagg cacaggacct cgtcgagcga
     2161 attcgtatgc agcgactggg tgccgcccag gaccgcggcc agggcctcca ccgccgtgcg
     2221 gacgacgttg ttgtagggct gctgggcggt cagcgagcag ccggccgtct gggtgtgaaa
     2281 gcgcatccac caggagcggg ggttcttcgc gccgaagcgt tcgcgcatca cccgcgccca
     2341 gatccggcgc gccgcgcgca tcttgcagat ttcctcgaag aaatcgatgt gggcgttgaa
     2401 gaagaaggag agccgcgggg cgaacgcgtc cacatcgaga cccttacgac ggatgacgtc
     2461 ctccacgtac tcgatcccgt cccggagcgt gaaggccagc tcctggaccg ccgtcgagcc
     2521 cgcctcgcgg atgtgatagc cgctgatgct gatcgtgttc cacttgggga cgtgctttgt
     2581 cccgaactcg atggtgtcgc tgatgagttt gaccgagggc tccggggggc acatgaaggt
     2641 cttctgggcg atgaactcct tgagcatgtc gttctggatc gtcccgccga tccggtccag
     2701 ggaaacgccc ttgacctccg cggcggcgca gtacatggcc cacaggatcg tcgcgggcgg
     2761 gttgatcgtc atggaggtcg ttacccggtc cagcggaatc ccgtcgatga ggaccaggaa
     2821 atcctccagg gtgtcgatcg ccacgccgca cttgcccacc tcgccccggg ccttcgggga
     2881 gtcgctgtca tagcccatca gggtgggaaa atcgaacgcc gtgctcaatc ccgtctgccc
     2941 gcttttcagc agcaggtgcc accgctcgtt cgtatcctgt gcgctcccca tccccgaaaa
     3001 catccggtag gtccaatagc gcccccggta ccccgtggcc tggtttcccc ggaggaaggg
     3061 atattcgccc ggcacgccga tgtcccgcgc gaaatccatg gcttggatgt cttccggcgt
     3121 gtagagccgc ttgatctcca ggtcggacac cgtcgaccag cgggccttct gatccggatt
     3181 ctccgccacc gaccgccgca cctcgtcctc ccactgacgg gtcaggcgcg cggattgttc
     3241 cagcgtctct tttgcatgca acacgtcttg aatcctcctt ggccttcctt gaaaccgatg
     3301 cgggggcata ccggcgggcg ggaatcggag cggctgcgcc ccgttcagcg tggttacgtc
     3361 aacgaaccgt ttccggaggg ttttttcacc agggaacaga ccggaccgtc attcctgccg
     3421 tccgcgcccg tcgaacaggc gtttttttgg cgcctacacg taacgcaaag gtcccggctt
     3481 gtcaagccaa atatcgtttt ctccagccct cgcacgactt tgtaacctgt tgattaccaa
     3541 aactaatccg gaccgggagc cagcccgggc ccgtcggacg cgggccggcg acgggggcgt
     3601 cgcggcccct ttcgaaggat gcagacgggg cccggcggac aaaggaaacc cggccgtgag
     3661 gccgggcttc cccgaatcga tccgtgcctg ccgttactga gaaaccacta ccacaggaaa
     3721 ccccagtccg cgttgctcag atagcctcca tggatgtcga tcgaggcccc gttgatgtag
     3781 gacgccttcg gcgaactgag gaacaggatg caatgcccga tgtcctcggg gctgccgatg
     3841 cggccggctg gaatcctggg cagcgcgtgt tcgtagacga gcttgatcgt cgcctcgtcg
     3901 agaccggccc gggcgtagat cggagtgtcg atccaccccg gcaggatcgt gtttgcccgg
     3961 atccccggga ggtcacggac gatgtgttgc gtgaatttca gcatggccgc cttcgccgtc
     4021 gaatagagga aatggttttg ggtgtgctcc acccgcgcgg cgctcgatga gatgttgagg
     4081 atcgacggat tggcggattt ccgcagcagc ggcgcaaagt gcttcaccat gagcatcggc
     4141 cccttcacca gcacgtcgta atgccagtaa aaatcgcttt cctgcatggc ctccatctgg
     4201 accagggtcc cccggccggc gttgttcacg agggtgtcca gccgcccgaa tctttcctcg
     4261 acgaaccggg ccgcggcggc aatatcggcc tcgttcgaca cgtcgccggg acgcgaaacg
     4321 aagcgctccc ccagatcatc gcgtgtccgg gccagggcgg ccgcctcgag gtcgaagccg
     4381 acgacggtcg ccccttccga gatgaacagc cgcgcggttc cgaatccgat ccccgacgcc
     4441 gcaccggtaa tcaagacaat gacatccttg aacatcgctt tccctccttt tatgaatatc
     4501 cgtcaatcca cgaccgccgc aacaaccatg tcggagaggc gctggtgccg caggaagctt
     4561 ttcccctcct ccatgagacc cgccgagaga aagacatacg tcaggtcccg ctccggatcg
     4621 acccagaaca tcgtggagcc ggccccgagg ccggcaaagg tgtcgggcga cgtgttgatg
     4681 ccgagcgggg tcggaaagac gccctcgccc cgcaggaaga aggtcaggcc gaggaacgcg
     4741 ggaatctccg gccatccgta catctcccgc atatagtcca tcaggtggtt cggttgatcc
     4801 cccgtctggt tggtggtggc gaggatcacc gtttcggggg acagcaggcg ccggccgttg
     4861 agctctcctc cccggcgaag catttccgcg aaaagaaata catcatgggc cgtcgagaca
     4921 ccgccgcccg aaggcatctc ggtatcctcc gcggcgatgt agttcaaggc ctcaatgagg
     4981 tcggggtcga agatcccccg ggtcttgtcc cggacgacga ccggcacgag ccgcttcttc
     5041 agacggtccg gcagtcccag ggccgtgtcc ttcatcccca ggggcgcaaa gacgtcttcg
     5101 gcgaggatcc tgcggaagga ccgcttcttc gcgtcgagcc gctggaccat ggccgccacg
     5161 agggcatggg ccgtcgtggg attgtaggta acgatcgttc cgggcatgaa aagaacccgt
     5221 tcgttgctca ggcaggcaac gacggcctcg atgttcccca gtttgtccac gggcagcgtg
     5281 aaggggattt ccgtgttcag gccggtccgg tgcgtcagca cgtgacccac ggtgatgttc
     5341 tgttttccct tgatgccaaa ctcggggagg atctcccgga tgggcgtcgt caaggtaaat
     5401 ttgcccgcct cgatgtccat gagcacccga accgtcgtca tctgcttggt cagggacatg
     5461 ataaagaaaa catcgtcctt gcgtgccgcg cgcttcttcg ccaggtcggt atggcccacc
     5521 gcttcatgca ggacgatttc gccgtggcgg gcgacgatga aaaccgcgcc gtcgtaaaga
     5581 ccctgggtgg tgtccgcctc gatgacctcc cggatgcgaa gcagccgatc cggatcgagc
     5641 cccaatttcc gggcatggcc cacgtccgcc ttgctcacct ttcccctcgc cataaaattc
     5701 cctccttccc tccttgagac tgtccgtgtt ggagaaacaa acgatttctt cccctcctgt
     5761 cttcacctgt cggttcgtaa aaataccccg ccctctttca agataggcct gtcgcagcgc
     5821 acgacacgga acgatcactt tttcttcttg acctctcgct catagagcca tttgtccggc
     5881 gtcacgacct gagcataacc ctggcgcttc gccttctcgc gggaaaggtt tttcatcccc
     5941 atcttcgtca cgatcttgtg aatctgcccc gtcagtcttg ggaacgtccc gccgaacttg
     6001 cacagccatc ccctccacgg gggtattgca gcttctagga ccttttcttc cagaactctt
     6061 ttgagaatca ccgccaccac atcttcaacg ctcaggatgc gcggtgcgga aaagccgacg
     6121 gcggccgcct cgtccccata aatgagttga gtctgcagct tggccgtatc gacgaggtcc
     6181 ggttcgatca gcgagacgtc gatgcctcgg ggcttcaatt cgtaggacac cgccaggctg
     6241 aatccccgca gggcaaactt ggaggccgag tagaggctgg tccctggaac cggcgcgaga
     6301 ccagcgaggg agcacatgtt gacgatatgg cctgagccct gcttgatcat ctgttctgcc
     6361 gcaaacctcg tccccaagat cgctcccttc gtattgatat cgatatgcag atcgatgtgc
     6421 tccaggggga attggtgaac gaacccctga atggcgaatc ccgcgatatt catgcaaaca
     6481 tcaagtttcc cccaggtccg gacaaccaca gcgacggctt ccttccacgc ttcggcatca
     6541 cgcacgtcca gcgtgtgcaa cttcacccga tccgcgttcc acttcagctc acgtgcgctg
     6601 tgttcaagag actccggatt gatgtccgtc gccaaaatgc gatacccgcg cccggacaag
     6661 accgtcgtca agtgtcgggc aataccactc ccgcagcccg taatcaaggc aacccgttcc
     6721 tgacccatac tccctccttt ctgttgagat tttgcctgca aagttaagaa ccaagagcga
     6781 accgagggac tgccatccgt aaccatgcac tcccgagggc cagccggaac ggtcgaacct
     6841 gcccgatcaa ggactgccgg cgagccccct caggatcatg cgcatctgcg tttggatcaa
     6901 ggcggcgatc ccgaaatcgg tgttttccaa aagtccccgc ctgttgatcg acaaggcccc
     6961 ctcgatgccg ctccagaggg cgtaggcgat ttgccgacgg tcgccgacgg gcttcatctc
     7021 gcccttccgg atgccctctt caatcaccga ctggacgagg gagatggccc tggtgagggc
     7081 ttgatccagc cgtgacgcca gctcttcctt gagtcccacc cgcttccagc ccaggtcgct
     7141 gaacgcgaag aggtcaaagt attccggatg ttccagataa aaccgggaaa aggcgtcagc
     7201 catgttttcg agtttcttta ggggggaggc cttttccccg cactcccgtt cgagaaaccg
     7261 gttgatcatt tgaaaggacg cctcgcagat ttcggcgtag atctcgtcga tcccgccgaa
     7321 ataatagtag atcgccccgg gtgaaagcgc ggccgccttc gcgatgtccc ggatgttcgt
     7381 cttgaaatat cccttcttca ggaacaccgt cttggcggcg cccagaatcc gttcgcgcct
     7441 ctccatcctt tccgattgtc ttcgcttcgc gacagccgcc tgcatgaacc ctcctcgaga
     7501 aatttgaacg ctgtttgaat aataaacggc gttcagaatg tcaagtcatt tccacaatcg
     7561 ttccttttgc catgatgacg tggcgttcac gtgatttact gaacgaccat atccatgcct
     7621 cggcgggaaa agttcccggc gggcacggcc caaactttcc gaaactggga gcgcccggcc
     7681 gtccgggcag gccgcctcgc gggaagcggg ctgttttatt gcacgcaatt ttgttggcaa
     7741 tcgccccttt tgctggtatg gatttggggc cgccgacaca ggaaattcga acagcggggt
     7801 cgaacccgca aggagggacg agacatggct gaatcgtttc cggtcgtcga agagatccgt
     7861 gccctctgtc gcccgcggtc cgtcgccgtg gtgggcgtcc cccggggcct gaaaaccggc
     7921 cggctctttc tgatggccct gcaggatcag ggcttccccg ggccgatcta cccgatcaac
     7981 ccccggatcg acgagatcga cggcctcaag gtctacccct ccgtcgcggc cgtgcccggc
     8041 gagatcgacc tggccatcgt tctcatcccc cacgatcagg tcatggactg catccatgac
     8101 tgcgtcgcca aacacgtccg cggcgcggtg ctcttcacgg cgggctacaa ggagacgggc
     8161 acggccgaag gcgcggccct ggaggcggag atcgtccgca tcgcccgcgc cggcggcatg
     8221 cgcctgatcg gaccgaacgg catgggtcta taccgcccgg ccgccggtct gtccttcttc
     8281 cctcagctcc cccgcgaaac ggggccggtg ggcctcatct cccacagcgg ctccctcacc
     8341 aacatcatcg gccggatggg cgcggagcgg ggcctccgct tcagcaccgt ggtcagcctg
     8401 ggaaacgaat gcgacctttc cgccggggac ttcctcgcct atctggggga ggacccggag
     8461 acggaggtca tcggggggta catcgaaggg atccgcgacg ggacgcggtt tctagccgcc
     8521 ctgaagcacg cgtcgcgccg gaagcccgtg atcctctgga aggtgggact gacgccggag
     8581 ggggcgaagg ccgccgcctc ccacacgggc gccctggccg gctccgaaga ggtctggcgg
     8641 ggcgtcgccc ggcagggcgg cgcgattccc gtggcggggt tcgaggcctg ggtggacgcc
     8701 ctgatgggct tcgccctcat ccggccgccc cggggcaggc ggatcgccat cctggccggt
     8761 cccggcggcc tgggggtttc cgccgcggag gcctgcggac gggccgggct cacgctggcc
     8821 gccatcgcgc ccgaaacccg ggaacggctc gcccggttcg tccccgcgac cggcacaagc
     8881 ttgggcaacc cggtcgacgt gggcatgtcc gcctcgctcg acgtcgaact ctacatcgag
     8941 gcggcgcggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acgttcgtct
     9061 ccttcatcct cttcctcatc cccggatttc cgaaggactc cctctgctac atcatcgggt
     9121 tgagccacat gaagacgtcc accttcgtcg tcatcgcgac gttcggccgt cttttcggaa
     9181 ccctcggtct gtcggtgggg gggaatttcg cccggggcgg gcagggtctc ctgctgctgc
     9241 tcgcgctcgg cggcgtcggc ctcgtcctgc tgctggccta tctcttcagg gaccggtggc
     9301 tccggttgaa gaaaaaaggc tgaacgccat tactttcggg tattcttctg cgcgtacatg
     9361 agttcgtccg cctcggacat caggtcgtcc agggtccggg gctgggcggg atcgaacagg
     9421 gcgacgccca ggctcagcgc gatctcgaac ctgttccttc cctccgcgtt caccgcctcg
     9481 atccgccgat tcaaccgcag cttcaggatc tccgcgtcgc gcagatggct gctcaccgcc
     9541 aatacggcga actcgtcccc tccgatgcgg gcgacgatgt ccgaggcgcg gaagacctcc
     9601 ttgaggatgg ccgccgtctg gatcagcgcc cggtcccctt cgtagtgtcc gaaccggtcg
     9661 ttgacggcct tcaggttatc catgtcggca aaatacagga gaaagccctg ccggacccgc
     9721 acggccagct tcaactgctg ctccgccagg gtcatgaatc ctcgccggtt gtagaggccg
     9781 gtcaactgat cgctgatcga cagccgccgc aactcctctt ccatccgttt gcggtcggtg
     9841 atatcgcgcg agacgacctg ccacccctcg atccgatccc cgtccaggat caactggctg
     9901 ttctgaccga accacatcgt ccgcccgtcc ttggcgacga tcggtaactc gctgtacgtg
     9961 ttcatcgtct tttcggcgaa ctgttccgtg aacatcgccg tgacgtcccc gcgatggtcc
    10021 ggcggaataa aatcgagata cgaccggccg agcagatccg aagcggcgta gccggtgatc
    10081 cgctcggtcg cggggttcac gaaggtgaag cacccccgcc ggtcgcagcg caggatgatg
    10141 tcgttagcct tctccaccat ctgccggtag cgttcctcgc cgacgcgcag cgccgaggcc
    10201 atcttcaccc ggtccgtgac gtcttccacg gccccgatgc cgccgaccac ctcgcgctcg
    10261 gcgttgtgca ggggattgac gttgaaggag acccagatgt ccgcgtccga ggtcatggcc
    10321 cgatagcgcc cctcgtaatg ggacggccgg ttgtcgaggg cgacgagcag gtgggggatg
    10381 acggccggct cgcgcaggct cgccagatcg agcccgacga tcctgtcccg gctggaccgg
    10441 acgatccggg cgaatcgctc gttgcaatcg gtgacgatga gcgcccggtc gtagatgaag
    10501 atgccgatcg gggcctggag gaagagggtc cggaaacgct cctcgctctc ccgcagggcc
    10561 tcctccatgc gtttgcgctc ggagatgtcc gggatattcc cctggatgcc gacgggcgcc
    10621 ttgcgggcaa tcgccgggcg ggacggccgg gcggcgtcct ccgcgcgccc gcgacgcagg
    10681 gcgcacagct cctcgatcag ttgacgcttc gtctttgcct cgtcccgcat ccccccgtct
    10741 ttccccctgg agcacttata agatgcatag ggttcataat cgaatcctca gaaatattca
    10801 agcttttttg cccggtccgg gcggcccgga cgcaccgggg cctttcaatc gtcaatctca
    10861 accggctgga cccgccagat cttctcgcag tactcccgga tcgcccggtc cgatgaaaac
    10921 ttccccatgc gcgcgacgtt caggatgctc atccgctgcc aagcgtcctg atcgagccag
    10981 gtccggccga cccggtcctg ggcttcgatg taggaggggt agtcggcgca gaggcggtag
    11041 tcgtcccggt cgcggatcgt ccgggtgagc ggacggaaaa gttcccggtc gttgcgcgag
    11101 aagaatccag cctccagcat ctcgagcgcc tcgctcaagt cgtcgttcgc cgcgatcgcc
    11161 ccgtcgggct gccagccctc ccgccacgaa cgctccacct cctccgcggt caggccgaag
    11221 aggaagaagt tctcggcccc cacctcctcc cggatctcga cgttcgcgcc gtcgagcgtc
    11281 ccgatcgtga gcgccccgtt catcgagaac ttcatgttgc cggtgccgga ggcctccttg
    11341 ccggccgtgg agatctgctc ggaaaggtcg gccgccgggt agatcaagtg ggcggtcttg
    11401 acgttgaagt tgggcaggaa ggcgaccttc agacgcccgg ccgccttcga gtcggagtgg
    11461 atcgtttccg cgacggcatg gaccagcttg atgatgagct tcgccatggc atagcccggc
    11521 gccgccttgc cgccgaagag aaccgtccgc ggcggccggt cgccgacctc gccgcggcgg
    11581 atgcggttgt agagcgtgat cacgtgcagg acgttcaggt gctggcgctt gtattcgtgg
    11641 atccgtttta cctggacgtc gaagagcgaa tcgggatcgg tcgcgatccc ggtgcggctc
    11701 tcgacaagct ccgccagccg cgccttgttt tcgcggttga tcgtccgcca gcgcacgcgg
    11761 aaagcggcgt cctccgcaaa gggctccagc gcctccaggc gggccagatc ccggatccac
    11821 gccgggccga tcgcctcggt gatgagtgcg gcgagcgccg ggttcgcgag ggcgacgaag
    11881 cgccgagggg tgacgccgtt ggtcacgttc aggaattttt ccgggccgat ccggttgaag
    11941 tcgttcagaa cctgcgcgcg cagaagctcc gtgtgcaggc gggcgacgcc gttgatccgg
    12001 tggctgccga cgcaggcgag atgcgccatc cggacgtagc gctccccgct ctcgtcgatc
    12061 agcgagagcc gccgggcgag gtcctcatcg cccggataat cgcgtcggat cccgtcgagg
    12121 aaacggcggt tgatctcgaa gacgatctcc agatggcgcg gcaggacgcc ggcgaacagg
    12181 ggcaccggcc acgtttcgag ggcctccgcg agcagggtgt ggttcgtgta gccgaagctc
    12241 cggcgggtga tctcccaggc ctcttcccag ccgagcccgt ggatgtccac cagcaggcgc
    12301 atcagttcgg cgaccccgat cgccgggtgg gtgtcgttga gctggatcgc gtatttttcg
    12361 tggaaccgcg tcaggtccgt ctcccgctgg aggaagatcc ggatcatgtc ccgcagggcg
    12421 caggagacga agaggaactg ctgcatcaga cggaggcggc ggccggcgac cggctcgtcg
    12481 ttggggtaca ggaccttggt aaggttctcg gagccgatct tgtcgtggac ggcgccgtag
    12541 taatcgccca cgttgaacga gcgcagatcg aaagaggtgg tcgcctccgc cttgaagagg
    12601 cgcagcatga tgaccgaggg cgaggcgtag ccggtgatca tcgtgtcgta ggcgacgccg
    12661 cggatcagcc ggtcggggat ccagcggatg cggtcgcggc cggcggcgtc gcggaccctc
    12721 tcggtacggc cgccgaaggg gatgtcgtag gcgatctcgg gacgcgggac ctcccagggg
    12781 ttgcccttgc gcagccactg atccgccacc tcctcctgcc acccgtcgcg gatctcctgg
    12841 gtaaagatgc cgaactcgta gcggatgccg tagccgatcg ccgggatgtt gagcatggcg
    12901 agcgaatcga ggtagcaggc cgcgaggcgc cccaggccgc cgttgcccag ccccggctct
    12961 tcctcctggt cgaccagggc ctggaaatcg agccccagcg ccgccaccgc ccggcggacc
    13021 tcgtcgtaca ggccgaggtt gatcaggtga ttttcgaggt ggggtcccag gaggaattcc
    13081 gccgagaggt agcagaccgt gcggctcgcc cgctcgtagt aggtgtgggc cgtcttcgcc
    13141 cactgggcga gcagccggtc gcggaccgtg taggccaccg cgaggtagta gtcgttcggg
    13201 gtggcgatgg gggcgaagcg cccctggatg cagacgaggt tgtcgaggat ggcctgcttg
    13261 atcctggcca cactgccgcc ggttctgatg ccggccttcc cgcgcgttcc cgccgacgtt
    13321 gccatggctt cctccctcgg gggcggacag gacgattccc ggatggcttc agggtgagac
    13381 ggcttaatcc acgcagaccc ggcgcgccgc cgggagcgga acccccgggg tgcgcaagcg
    13441 gtttgatttt acataacccc gtccgccccg gattgcaaaa gaaaaaatgg gatgacgcgc
    13501 gccactccgg attctcatac cgattgacat ccggccggcg ggcggtgtat gatcgggacc
    13561 gtgcgggaga ggcgcgttcg tccgtcgccc gccgaagggc tttgcagtga gggacacgga
    13621 tgccgctgta cgattttgaa tgcgaaaaat gccaccatac cttcgaggcc atcctccgga
    13681 tggacgagcc gtgggacacc ctggcctgcc cctcctgcgg caggaagaaa gtacggaaaa
    13741 aggtgatgcc gatccggacg aacgcctggt ccagcttcct cgacggcatg gaaaagcgcg
    13801 tcaacccgca caaattcaag taggacgacc cgattcagaa gatacacggt catatcggcg
    13861 gactgaagcc ggaccagctc cggcggatca cgcagctcta tcgtcgccgc ctgccgcccc
    13921 ggcgcgtcgt gacccatgaa ctggcccgcg agctgacgga gctctcccgc gagctgaacc
    13981 gccagatcgg ccttctcgtg agccggcagg gcgaggtcgc gggcgttatc gtcggcaccc
    14041 accagcagat cgtcatcccc gccctggaca gcttccgcgc cgcctcgacg cgcttcaagg
    14101 ggctccgcct gatccacacc cacttgaacg ccgaaggcct gacccacgac gacctgaccg
    14161 acctggccct cctgcggctc gacctgatct gcgccgtggt ggccgccgcg gacggcctgc
    14221 cgggcatggc ccacgccgcc cacctgatcc cggagaaccc cgccggcgag ttctggcagg
    14281 tccttccgcc cgcgcggccg tcggagctgg acctggattt cctggccttc atccaggccc
    14341 tggaggggga gttccaaaag cagcagcgca cccggcgcgt ggaggccgcg gaccgggcgc
    14401 tcctggtccg cgcggagacc gaccccaacg gggaaccgga ggcctgcctc gacgaactgc
    14461 gcgaactggc gcattcctgt ggggtggagg tcttcgacgc cgtcctccag taccgggacc
    14521 ggatggaccc ccgcttcgtc atcggccggg ggaaactcgc cgacgtggtc atccgcgccc
    14581 tgcagatcgg cgccaacctc ctcatcttcg atcacgaact gacggccgcc caggcccggt
    14641 cgatcagcga cttcaccgag ctcaaggtca tcgaccggac acaggtgatc ctggacatct
    14701 ttgcccagcg ggcccacagc cgggagggga agatccaggt ggagctggcc cagctcaagt
    14761 atcgtctgcc ccgcctggtc aagatggacg ccgccctctc gcggctcacc ggtggcatcg
    14821 gggcgcgggg gccgggggag atgaagctgg agatcgaccg gcggcgcgtc cgggagcgga
    14881 tccaccgcct cgagcaggac ctcaagggga tcgggaaggc gcgaaaccag cggcgcgtga
    14941 ggcgcgagcg gacggggctt cccgtcgtct ccatcgtcgg ctacacgaat gcgggcaagt
    15001 ccaccctgct caacacgctc acgaagagcc gcgtcctgac ggaggaccgc ctcttcgcca
    15061 ccctggaccc gaagagctcc cgcctgcgtt ttccccggga cacggaggcg gtgatcaccg
    15121 acacggtcgg cttcatccgc gacctgccgc cggacctctt cgccgccttc cgcgcgaccc
    15181 tggacgaact gaaggaggcg gattgcctcc tgcacgtcgt cgacgccagc aacccccgct
    15241 tcgagaatca gatcgccgcg gtggagaaga tcctcgccga cctttcgatg accgggaaac
    15301 cgaccctgat ggtgcttaac aaggcggacc gcgtcgccga caaggaccgg ctggccgcgc
    15361 tctcccgccg gtacgggacc gtggccgtct cggccctcga tccgggcacc cttcccggac
    15421 tcctcgaagc cctggagcgc ctgatcggcc ggctgtcccc gccgggcgtc ggcgccatga
    15481 tcgaatcggc ggccggatag gggcctgtcg aaaaccgagc gagaccctcc gtatgccgga
    15541 aatcgcctac acgccgctgg gacgcaatgc cctcttcacc gggctgatcg gtcatggtgc
    15601 ggccctcctg gtcggcatca tgcccgcggc cggcggggaa cgcttcttcg gcatcgtctt
    15661 tcccctcgcc tgcctcgccc tcctcctggg cggttatgcg gaattgaagc gaggcggacg
    15721 ctgccccttg agcgactggc ggttctacgg catcgcggcc gcaacggttg ttccggtgct
    15781 tgggcctttc gtggttctcg gcttgctctt cggtttccag aatggggtgc agggcgaacg
    15841 catcggcccg ctcggcctgt ttcccgccct ggtcaggcta agggcgaacg ggctggttct
    15901 tttcctgtgg attgtctttc tcgtggtcct gtttgccttt accggcagcc gggatgaccc
    15961 ctacttcaag aggcatcggg atccttccgt caggaacgcg ccacaatcgc tttcggatgc
    16021 ctgtcgctac gggctttccg gacgtgcgcc gcaaccggtc ggcctcgacg ggaaggcttg
    16081 ccgccaatcc gtgtaggacg gaaagaggag ggaaaatgaa aaaattgctg ctggcgatcc
    16141 tctggtccca ggtcattttc tccgtggccg cggacccggc cggcgccggc caggttacgg
    16201 ttcataaccg ccacgaccgc gactgggatg tcggcgtcgt cttcacccag agcgcggccg
    16261 aggatatccg gagcgtcatc gtggagcccg gtcgcacgga aaccgtcgtc aactggtact
    16321 ggggcgtcgg cgccatcaat gcccaggagc tgagagcgga cccgaaccat ctcttccgcc
    16381 gttgcgaata cgccgtaacc ggcatggtgt ggcccggccg gggcttcacg ctcaccctcg
    16441 gaagcgacgg aacctgcacc ttcacggagc cgtgatcgcc gccggcacg
//
LOCUS       DAOOLJ010000035        16485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3699_length_16485_cov_18.397079, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000035 DAOOLJ010000000
VERSION     DAOOLJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_16485_cov_18.397079"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLW16_06845"
     CDS             complement(<1..341)
                     /locus_tag="PLW16_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPB70659.1"
                     /translation="MEGKRHTSREYERELQEIKEGLLYLGALTERAIARGVTALLEKD
                     SALAGQVICEDDQIDTLDVELEERCIRLLALRQPAARDLRFITTAIKINGHLERIGDM
                     AANIAEKVIIL"
     gene            complement(353..1114)
                     /gene="pstB"
                     /locus_tag="PLW16_06850"
     CDS             complement(353..1114)
                     /gene="pstB"
                     /locus_tag="PLW16_06850"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPB70660.1"
                     /translation="MEKDKIIRAKNVDFYYGSFQALTGVTMAFEKNRVTALIGPSGCG
                     KSTLLRLLNRMNDLIDGARVEGEILFEGRNIYAPEVDPVEIRRRIGMVFQKPNPFPKS
                     IFKNITYAPKLSGVHRKSDLEALVEESLRQAVLWDEVKDILDRSAMTLSGGQQQRLCI
                     ARALAMKPDIILMDEPTSALDPISTARIEELIDELKQRYTIIIVTHNMQQAARVSEYT
                     GFFYIGKLIEFGPTEKIFTNPSVKQTEDYITGRFG"
     gene            complement(1117..1998)
                     /gene="pstA"
                     /locus_tag="PLW16_06855"
     CDS             complement(1117..1998)
                     /gene="pstA"
                     /locus_tag="PLW16_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPB70661.1"
                     /translation="MNAAAPSTSLKWRYVRQGLFFGVVRLSAFLITATLLLILAYIFM
                     NGFRAISWDFLTLPPTDAMTRGGIMPAIVGTLYLTVGAIAVALPLGVASAVYLTEYAR
                     QGLAIRVLRIGVNCLAGVPSVVFGLFGLGLFVVFLRFGSSILAGALTLGILILPTIIG
                     ASEEALKAVPQTFREASLALGVSKWRTISRIVLPNALPGILTGSILGIGRAAGETAPI
                     MFTAATFFTAALPRSVFDEVMALPYHIYVLATAGTQIDETRPLQYGTVLVLVALVLGI
                     DLVAILIRSAMRKKKRW"
     gene            complement(1995..2882)
                     /gene="pstC"
                     /locus_tag="PLW16_06860"
     CDS             complement(1995..2882)
                     /gene="pstC"
                     /locus_tag="PLW16_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPB70662.1"
                     /translation="MTRQFKEILIKNLFALLAFVAVLVLGLIVVFLFREGLPLFRLVS
                     PGQFLFGTAWYPTYDPPEYGIWPLIVGSFTVTLFSCLIAVPLGVLSAVYISEIAPAHL
                     KNVLKSVIELLAGLPSVVLGFFGMVVIAPWLQETFDLPTGLNIVNASLMLAIMAIPTI
                     TSISEDALYAVPREFKEASYALGATKFETIARVIVPTALSGISTAVILGMARALGETM
                     VVLMAAGGAAAVPESLFDSVRPMTASIAAEMGEAPFRSGHYHALFATGMVLFGITLAF
                     NLIADAISNRFKEVGSGTL"
     gene            complement(2939..3784)
                     /locus_tag="PLW16_06865"
     CDS             complement(2939..3784)
                     /locus_tag="PLW16_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB70663.1"
                     /translation="MHGILKKLAAVSLGLLFCTGTALAETVVIKGSTTVLPIAQVTLE
                     AYMKARPGVNISLSGGGSGDGIKALIDKSTHIANSSREIKASEIALAKSKGIAPTETV
                     VAVDAIVPIVHPKNRVRDLSIDQLSQIYQGKITNWKDVGGEDLKIVVISRDSSSGTFE
                     AWGEMVLHKAKVAQRAQMQASSGAIVQAVSKNRYAIGYVGLGYLNRSVKALTVGGVPA
                     TAPNAIAKTYPISRPLYMYTDGKPAGETARFLQFVLSPAGQKIVEKAGFVPLTDARKP
                     PKKGK"
     gene            complement(3906..4613)
                     /locus_tag="PLW16_06870"
     CDS             complement(3906..4613)
                     /locus_tag="PLW16_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB70664.1"
                     /translation="MTRKIILIADDERDIVELIAYNLEREGFSVLKAYDGKRAWDRTR
                     QSKPDLVLLDLMMPGTPGMDVCRAIRGHAETSAIPVIMLTAKAEPVDKILGLELGADD
                     YITKPFHVRELIARVRAVLRRTKPRPEDETPEAFDFRGLHIDFGTYRVTVDGRPVELS
                     TLEFKLLKFLTGRPGRVFSREQLLDRVWGDEAFVEPRTVDVHISRLRAAIEADKETPR
                     FILTVRGIGYKFADADA"
     gene            complement(4717..5715)
                     /locus_tag="PLW16_06875"
     CDS             complement(4717..5715)
                     /locus_tag="PLW16_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPB70665.1"
                     /translation="MVDSLYFVIFLVGVALVFDFLNGFHDSANAISTIVSTRVLSPRY
                     AVLWAAFFDFAAVFFIGVPVAKTIGTGIIDPAIVNNYIILAALGGAIIWNIITWWFGL
                     PSSSSHALIGGLIGAGIMKGGTGTLVLSGITKTSLFIVVSPVLGLILGWVLMVIVLNI
                     SRNSNVARSEKIFRKLQLVSAAVYSMGHGTNDAQKTMGIIAIILFSKGLLGPTFYVPF
                     WVVITCYTVIALGIMSGGWRIVKTMGTKITKLKPIGGFCAETAAAISIIGASLAGIPV
                     STTHTITGAIVGVGATRRLTAVRWGVAGSIVWAWILTIPLSALISAILYILINMIG"
     gene            complement(5708..6328)
                     /locus_tag="PLW16_06880"
     CDS             complement(5708..6328)
                     /locus_tag="PLW16_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPB70666.1"
                     /translation="MRFIPREEKFYDLFEELIAKIEEGGRLFSEMLEDYEHAETRLTK
                     LKDIEHEADIITHRTYEKMHKTFLTPIDREDIYALVNKMDSILDIIEAAAIRMFLYKI
                     KAPTAECVKQAQLINASIAKVKQVVHALRDMKNAQMILDACVEINTLENEGDYLLRQA
                     MTHLFENETDAIELIKWKEIYERIEEALDTCEDVSNIAEGIVLKNG"
     gene            complement(6471..6974)
                     /locus_tag="PLW16_06885"
     CDS             complement(6471..6974)
                     /locus_tag="PLW16_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB70667.1"
                     /translation="MTDPALRFRTDLRPADREAIRRLVDGTGFFSEEEVAIALELVDE
                     RLRKGGDSGYHFLVAGQGGRISAYACYGPIPGTRNSFDLYWLVVDASRQRQGIGGRLL
                     ARSESRMAALGARRVYADTSARPQYAPTRAFYEASGYAPTAFLEDFYAPGDGKVIYVK
                     VLTTDSG"
     gene            complement(6967..7977)
                     /locus_tag="PLW16_06890"
     CDS             complement(6967..7977)
                     /locus_tag="PLW16_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70668.1"
                     /translation="MNVVVLHTPVGTEASRDEADTDIQREAVLDALGALGHRAVALPF
                     SLNLEETAARLRALAPHVVFNLYEPLTGEGRLIHLAPALLDQLRLPYTGNPTEALFLT
                     SNKLLGKRLLAHAGFKTPPWRTPRDAATSEPMPPGRWIVKSVWEHASIGLDDDAVFTT
                     GNAKDLDETLRTRGDGPGGDCFAEAYIDGREFNLSLLAGKNGPEVLPPAEIRFDDYPP
                     DKLRIVGYRAKWEEDTFEYRHSPRSFDFPPADGPLLETLTDIARRCWNLFALAGYARM
                     DFRVDAAGAPWVLEINANPGIAPDSGFVAAAGQAGFAYPRMIGRILQDAVTFRSTGGA
                     RD"
     gene            complement(8034..8109)
                     /locus_tag="PLW16_06895"
     tRNA            complement(8034..8109)
                     /locus_tag="PLW16_06895"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8074..8076),aa:Ala,seq:cgc)
     gene            8350..9432
                     /locus_tag="PLW16_06900"
     CDS             8350..9432
                     /locus_tag="PLW16_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPB70669.1"
                     /translation="MAKIDAFFQLMHEQGSSDLHLVSGQQPALRIRGEIERVKYNVLE
                     NDELKAMLYEIAPEHKIKQFEETGDIDFAYEIPGLARYRANFFQQKYGVAAVFREIPS
                     KILTCQDLGLPPVIGKLASLPRGLVLVTGPTGSGKSTTLAAIIDEANRLRKDHIITIE
                     DPIEFVHQSQGCIVNHREVGVHTKSFTAALRGALREDPDIILVGELRDLETISLAVEA
                     ASTGHLVFGTLHTTSAAKTIDRVIEVFPSEEQMQIRSTLADGIRAVIAQVLFKRIDKK
                     GRCAALEILIANVAVRNLIRESKTFQIPSMIQTGRKYGMQLLDDGIMDLLNKGWISAD
                     EAYAKCNDKAKFRPFLKTPPADFTEV"
     gene            9452..10627
                     /locus_tag="PLW16_06905"
     CDS             9452..10627
                     /locus_tag="PLW16_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPB70670.1"
                     /translation="MRKQEIDYILGRMLDTYGNVSDLNITVGKPFQVETSGQLSGVEL
                     DPPVPELTPFQTEIFALNLINQDRRLTETLLAEGSCDSSYALPGKARFRVNIFSQRGN
                     YSIVLRKLETRIPTFEDMNLPEAFHKMAEEKNGIILVTGATGSGKSTSLAALLNDIND
                     RKSIHIVTLEDPVEYVHPHRKATFNQREMGTDFDTFANGLRAALRQAPKVILVGEMRD
                     RETVEIGLSAAETGHLVLSTLHTVDAGQTINRILGMFSNEEETQIRIRLADTVRWIVC
                     QRLLPKEGGGRVAAFEIMGSNLRTKDTILHGESEGKTFYEIIEAGKAFGWTTFDDYII
                     NLYEQGLISEETGRAYASNKGIVGRGIDSVKSSKGEKTTDLGKLEVDKGYGKPKPRF"
     gene            10705..11214
                     /locus_tag="PLW16_06910"
     CDS             10705..11214
                     /locus_tag="PLW16_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70671.1"
                     /translation="MDEDKSAGQAAENYNASDKPFDFLEAGGATAIVCEADPEKRAML
                     NAALKSLDYRITEAADAKDALKRMRFHIYDVAVIDERFDAENPDDNAVLKYLAGLNMG
                     IRRQTFVALVTERFRTMDNMAAFTKSVNVTINATNLDNATTIIKEGVEDNRAFYHIFR
                     ESMKKVGRA"
     gene            complement(11240..12220)
                     /locus_tag="PLW16_06915"
     CDS             complement(11240..12220)
                     /locus_tag="PLW16_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB70672.1"
                     /translation="MSQPTYEDEQMRLEAEKCMRDGRFEDAVALYRTLVAAHPGEESY
                     LLALAWACHDQGAWEEAIDCFERLLDRELGRKVFTGFGFDELVRIYKEHGLHDRLVEV
                     CGRACAAQPEDYALLAELGEACLLAAQPAEAAAAFRKMAEMEPDDAMAFCRLGNALIA
                     AGKFTEAETAYGRAAALEPDTGAVFFLRLADAYLKAGETERAETAARHCLASRPDEPL
                     YHCTLGDILVRRGRLEEARGCYDTAVALRPEDAGAYYNRLGNALTRDGRHRQALEAFR
                     AAVAAAPDTPALYERLATACRALGMDEQARDAELRVRINKKGPRDNLTGR"
     gene            complement(12416..12622)
                     /locus_tag="PLW16_06920"
     CDS             complement(12416..12622)
                     /locus_tag="PLW16_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70673.1"
                     /translation="MALDEPNENDEVFKNDGLTFIIEKALWEQAKPITVDFITSPMGS
                     GFKLSSNIAASGGCGTSCSTSCSC"
     gene            12979..13479
                     /locus_tag="PLW16_06925"
     CDS             12979..13479
                     /locus_tag="PLW16_06925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB70674.1"
                     /translation="MDFEIRPCSETDAGILAETIRAAFRDVAERFGLTRENAPRHPSN
                     CSADWVEKDMARGVVYYMLEDRGRASGCVALEMANAEVGYLERLAVLPERRRRGLGRV
                     LVAYALSEAARRGARRVNIGMIAEQAELKDWYRGLGFVEGETREFAHLPFRVTFLSQG
                     IQGEEP"
     gene            13476..13904
                     /locus_tag="PLW16_06930"
     CDS             13476..13904
                     /locus_tag="PLW16_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB70675.1"
                     /translation="MSSHIHHVHIFASNLDRTIEFYRDFFGGTVVLDMELAGARNVFM
                     RVGTGRIHFYDQPPRFSGRGSIHHFGIQTDDVEAVVNRMKAKGVVFTKGITDAGFWKY
                     IMVPAPDDILIEVFQVDRAQIPADLLGYFEWNDPRKELEA"
     gene            13901..14320
                     /locus_tag="PLW16_06935"
     CDS             13901..14320
                     /locus_tag="PLW16_06935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB70676.1"
                     /translation="MSDYGTYLKRLQAGELHLNPFLAFLDMRPEAMDDGYARFRMPIR
                     PEYLQGAGNMQGGMIVAMADEAAAHAIMTLLKPREGLTTIELKSNFLAPVRAGDLVAE
                     ARVFKKGRTLMIADCTVTDDRERPVLRLSATFLIIRE"
     gene            14503..15618
                     /locus_tag="PLW16_06940"
     CDS             14503..15618
                     /locus_tag="PLW16_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HPB70677.1"
                     /translation="MQPFEPQQLPIDDIPWDALIPLIGRANRSLAYYDGILYGLANPD
                     VLLSPLTTREAVLSSKIEGTQATLGEVLKFEAGAAPAEESRVRDIEEIMNYRRAMRRA
                     EEELKVRPFSLNLLKELHAVLLDGVRGRFEARGQFRSSQNWIGIPGTPIEEAQFVPPD
                     PAKMIEHLYGWEKYYHFDRPDPLVQLAIIHAQFELIHPFLDGNGRLGRMIVPLFLFER
                     RLISSPVFYISAYLEAHRDAYAAGLRSLSDKTPASWAHWIGFFLRAMDEQAKENAKKA
                     QAIIDLYGRLKERMIDLTHSQYAVPLLDHLFDQPVFTSSLFDERSGMPSKPMVMNMLG
                     RLKKAGILKVVREGSGRRPQILALAELINVCEGADVI"
     gene            15628..15846
                     /locus_tag="PLW16_06945"
     CDS             15628..15846
                     /locus_tag="PLW16_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70678.1"
                     /translation="MPFELGKQQEYIGNPLVVAQEHNDEADFYLSHGTAMEIHGMVMQ
                     PRLVFHITALKESGDSQGPLQPIRPTLI"
     gene            16038..16253
                     /locus_tag="PLW16_06950"
     CDS             16038..16253
                     /locus_tag="PLW16_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70679.1"
                     /translation="MAKAIFTTKITPSYNDLPELMYHFPKTYLRQANLTVGDWIIYYE
                     PRREDISPAGHAGRQCYFATARVTHIK"
     gene            16263..>16485
                     /locus_tag="PLW16_06955"
     CDS             16263..>16485
                     /locus_tag="PLW16_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70680.1"
                     /translation="MKDHYYAFVENYVEFSAPVPFKIGSFYYESGLKKADGSTNKGAF
                     GRAIRIIQDDEYQAIVAAGMAQINEQTVRD"
BASE COUNT         3147 a         4967 c         5277 g         3094 t
ORIGIN      
        1 ttcaggatga tcaccttctc ggcgatgttg gccgccatgt cgccgatgcg ctccaagtgg
       61 ccgttgatct tgatcgccgt ggtgatgaag cgcaggtcgc gggcggcggg ctgcctcagg
      121 gcgagcaggc ggatgcagcg ttcctccagc tccacgtcga gggtgtcgat ctggtcgtcc
      181 tcgcagatga cctgtccggc cagggcggaa tccttctcca ggagcgccgt gaccccccgg
      241 gcgatcgcac gctccgtcaa agcccccaga tagagcagcc cctccttgat ctcctggagc
      301 tcccgttcgt actcccggct cgtgtgccgt tttccctcca tggcgtccct ccttaaccga
      361 agcggccggt gatgtagtcc tccgtctgct tgacggacgg gttcgtgaag atcttttccg
      421 tcgggccgaa ttcgatcagc ttgccgatgt agaagaagcc cgtatactcg gagacacgcg
      481 ccgcctgctg catgttatgg gtcacgatga tgatcgtgta ccgctgcttg agttcgtcga
      541 tgagttcctc gatccgtgcc gtcgagatcg gatcgagggc cgacgtgggc tcgtccatca
      601 ggatgatgtc cggcttcatg gcgagcgccc gggcgatgca gaggcgctgc tgctggccgc
      661 cggagagggt catggcggac cggtccagga tgtccttgac ctcgtcccac aggacggcct
      721 gacggaggct ttcctccacg agggcctcga gatcactttt ccggtgcacc cccgacaatt
      781 tgggtgcata cgtgatgttc ttgaagatgg atttcgggaa ggggttgggc ttctggaaca
      841 ccatgccgat ccgccgccgg atctccaccg gatccacctc cggggcatag atgttccgtc
      901 cctcgaacag gatctccccc tccacccggg cgccgtcgat caggtcgttc atccggttga
      961 gcaggcgcag cagcgtggat tttccgcatc cggagggtcc gataagcgcg gtgacgcggt
     1021 tcttttcgaa ggccatcgtc acaccggtca gcgcctggaa cgaaccgtaa taaaaatcca
     1081 cattttttgc acgaattatc ttatcctttt ccatctttac caccgctttt tctttctcat
     1141 ggcgctgcgg atgaggatgg cgacgaggtc gatccccagg accagggcca ctaggacgag
     1201 gaccgttccg tactggaggg gccgggtctc gtcgatctgg gtccccgccg tggccaggac
     1261 gtagatgtgg tagggcaggg ccatgacctc gtcgaagacg gatcggggca gcgccgccgt
     1321 gaagaaggtc gccgccgtga acatgatggg ggccgtctcg cccgcggccc gcccgatccc
     1381 gaggatcgaa ccggtcagga tgccgggcag ggcgttcggc aggacgatcc gggagatcgt
     1441 gcgccatttg gagacgccga gggccagtga ggcctcgcgg aacgtctggg gcacggcctt
     1501 cagggcctct tcggaggccc cgatgatcgt gggcaggatc aggatgccca gcgtgagggc
     1561 gccggcgagg atgctggagc cgaaccggag aaagaccacg aaaagaccga ggccgaacag
     1621 gccgaagacc acggagggga cgccggcgag gcagttcacg ccgatgcgga ggacgcggat
     1681 cgcgagcccc tggcgggcgt attccgtcag atagacggcc gaggccacgc ccaggggcag
     1741 ggcgacggcg atcgcgccca cggtcaggta gagggtgccg acgatggccg gcatgatgcc
     1801 gccccgcgtc atggcgtcgg tcggcggcag cgtgagaaaa tcccagctga tcgcccggaa
     1861 gccgttcatg aaaatatagg ccaggatgag caggagcgtg gcggtgatca ggaaggcgga
     1921 caggcggacg acgccgaaga aaagtccctg ccggacgtag cgccatttca gggaggtcga
     1981 gggtgcggcg gcattcatag cgttccggac cccacttcct tgaaccggtt ggagatggca
     2041 tcggcgatca ggttgaaggc cagcgtgatg ccgaacagga ccatccccgt ggcgaacagg
     2101 gcgtggtaat ggccgctccg gaagggcgcc tcgcccatct cggcggcgat gctggccgtc
     2161 atgggccgga ccgaatcgaa gaggctttcc ggaaccgcgg ccgccccgcc cgccgccatg
     2221 agcaccacca tcgtctcccc gagcgcccgg gccatcccta ggatcacggc cgtcgagatg
     2281 cccgacaggg ccgtcgggac gatcacccgg gcgatggtct cgaacttcgt cgccccaagg
     2341 gcataggacg cctccttgaa ttcgcgggga acggcgtaca gggcgtcctc cgagatgctc
     2401 gtgatggtgg ggatcgccat gatggcgagc atcagggagg cgttgacgat gttcaagccc
     2461 gtgggcagat cgaaggtttc ctgcagccag ggcgcgatga cgaccatgcc gaagaagccg
     2521 agcacgaccg acggcaggcc ggccagaagc tcgatgacgg atttgaggac gttcttgagg
     2581 tgcgccgggg caatctcgga gatgtagacg gcggagagca cgccgagggg aacggcgatc
     2641 aggcaggaga agagcgtgac cgtgaaggac ccgacgatga gcggccagat cccgtattcc
     2701 ggggggtcgt aggtggggta ccaggccgtc ccgaagagga actgacccgg ggagaccagc
     2761 cggaacaggg gcaacccctc ccggaacagg aagaccacga tcaggcctag cacgagtacg
     2821 gcgacgaagg ccagcagggc gaagaggttc ttgatgagga tttccttgaa ctgtcgggtc
     2881 atgacggatg cttcaatcac tttcggttga gaggggggct gatgccggcg agcggcgatt
     2941 atttgccctt cttcggcggt ttcctggcgt ccgtgagggg cacgaagccc gccttttcga
     3001 cgattttctg cccggccggg ctcaggacga actggagaaa gcgggcggtt tccccggccg
     3061 gctttccgtc cgtatacatg tacagcggtc gggagatcgg atacgtcttg gcgatggcat
     3121 tgggggccgt cgccgggacg ccgcccaccg tcaaggcctt gacgctccgg ttcaggtagc
     3181 cgaggccgac atagccgatc gcgtagcggt ttttcgagac ggcctggacg atcgcgccgc
     3241 tcgaggcctg catctgggcc cgctgggcga ctttcgcctt atggaggacc atctctcccc
     3301 aggcctcgaa ggtgccggag ctgctgtccc gggagatgac gacgatcttg aggtcttccc
     3361 cgcccacgtc cttccagttg gtgatcttgc cctgatagat ctggctcaac tggtcgatgg
     3421 acaggtccct gaccctgttt tttggatgga cgatggggac gatggcatcg accgccacca
     3481 cggtttccgt gggggcgata cccttcgatt tggcgagggc gatttccgag gccttgattt
     3541 cccgagatga attcgcgatg tgggtcgatt tgtcgatcag ggccttgatg ccgtctccgg
     3601 agccgccccc cgacagggag atgttgacgc ccggacgcgc cttcatgtag gcctccaggg
     3661 taacctgggc gattggcagg accgtcgtcg atcctttgat gacgacggtt tccgccaggg
     3721 ccgtcccggt gcagaaaagc agtccgagcg acaccgccgc cagttttttc agaatgccgt
     3781 gcatcgtggt ttcctccgtt tctttttttg aggcagggta acggatcatt gtgacaattt
     3841 gatgaaggcg gtgtgaagaa acggtgaacg gtcgcgtcgg gaaacatccg ccggtaaagg
     3901 gcttttcagg cgtcggcatc cgcgaatttg taaccgatgc cgcgcacggt caggatgaac
     3961 cgaggggttt ccttgtccgc ctcgatcgcg gcccggaggc gactgatgtg gacgtccacg
     4021 gtccggggtt ccacgaaggc ctcgtctccc cagacccggt cgagaagctg ttcccggctg
     4081 aaaacccggc ccggccggcc ggtcagaaat ttgagcagct tgaactcgag cgtgctcagt
     4141 tcgacgggcc tgccatccac ggtcacgcga taggtcccaa aatcgatgtg gagtccgcgg
     4201 aagtcgaacg cctccggtgt ctcgtcttcc ggacgtggtt tcgtgcgacg caaaaccgcc
     4261 cggacgcggg cgatcagctc gcggacgtgg aagggtttgg tgatgtagtc gtcggccccc
     4321 agctccagcc ccaggatctt gtccaccggc tcggccttcg cggtcagcat gatgacgggg
     4381 atggccgacg tctcggcatg cccgcggatc gcccggcaga catccatgcc cggcgtccct
     4441 ggcatcatca ggtcgaggag gacaagatcc ggcttcgact ggcgggtccg gtcccaggcc
     4501 ctctttccgt cataggcctt caggacggag aacccttccc gctcgaggtt gtaggcaatc
     4561 agttcgacga tgtccctttc atcgtcggca atcaggatga tcttccgcgt catgcgcgat
     4621 cgctcctccc ctactctccc taacacacac ccgaccgttt gaaaaacgct ttttgatccg
     4681 gtttgttgaa ggcctgttcc cggtggggga cgaggattat ccgatcatgt tgatcaggat
     4741 gtagaggatg gccgagatca gggcggaaag ggggatcgtc aggatccaag cccagacgat
     4801 gctgcccgcc accccccagc gcacggccgt caggcgccgc gtcgcgccga cgcccacgat
     4861 ggcgcctgtg atggtgtgcg tggtgctgac cgggatgccg gcgagcgacg cgccgatgat
     4921 cgagatcgcc gccgccgtct ccgcgcagaa cccgccgatg ggcttcagct tggtgatctt
     4981 ggttcccatg gtcttgacga tgcgccagcc gccggacatg atgccgaggg cgatgacggt
     5041 gtagcaggtg atgacgaccc agaacgggac gtaaaaggtg gggcccaaaa gccccttgct
     5101 gaagaggatg atggcgatga tgcccatcgt tttctgcgcg tcgttcgtgc cgtgacccat
     5161 gctgtagacg gccgcggaca cgagctgcag cttccggaag attttttccg accgggccac
     5221 gttggaattg cggctgatgt tgagcacgat gaccatgagg acccagccca ggatcaggcc
     5281 gagcaccggg gagacgacga tgaacaggga ggtcttggtg atcccactca ggaccagggt
     5341 gccggtgccg cccttcatga tgccggcgcc gatcagcccg ccgatgaggg catgggagga
     5401 gctgctcggc aggccgaacc accaggtgat gatgttccag atgatcgcgc cgcccaaggc
     5461 ggccaggatg atgtagttgt tgacgatcgc cgggtcgatg atgcccgtgc cgatcgtctt
     5521 ggccacgggg accccgatga agaaaaccgc ggcgaaatcg aagaacgccg cccacaggac
     5581 ggcgtagcgg ggcgaaagaa cccgcgtgga cacgatggtc gagatggcgt tcgccgagtc
     5641 gtggaagccg ttcagaaaat cgaagaccag tgcgacgccg accagaaaga tcacaaaata
     5701 cagggaatca accatttttc aggacgatac cttcggcgat gttggaaaca tcctcgcagg
     5761 tatccagcgc ttcctcgatg cgttcgtaga tttccttcca tttgatgagt tcgatggcat
     5821 ccgtttcgtt ttcaaacaga tgggtcatcg cctggcggag gaggtagtcg ccttcgttct
     5881 ccagcgtgtt gatttccacg caggcgtcca ggatcatctg ggcattcttc atatcgcgga
     5941 gcgcgtggac gacctgcttg accttggcga tggaagcatt gatcagctgg gcctgtttga
     6001 cgcattcggc agtcggggcc ttgatcttgt agaggaacat ccggatggcc gccgcttcga
     6061 tgatgtcgag gatgctgtcc atcttgttga ccagggcgta gatgtcttcc cggtcgatag
     6121 gcgtgaggaa ggtcttgtgc atcttttcgt aggtgcggtg ggtgatgatg tccgcctcgt
     6181 gctcgatgtc cttgagcttg gtgaggcggg tttccgcgtg ctcgtaatct tcgagcattt
     6241 cgctgaagag ccgcccgccc tcttcaatct tcgcgatcag ctcctcgaag aggtcgtaga
     6301 atttttcttc cctggggatg aatctcatgc gttgaccctc ggaacaggcc ctttctctcg
     6361 ggcgcgtccc atttggaccg tcccggaaaa agccggttgt tctctactca ataatggtgg
     6421 aaaaagcaat ccctttccgc agcgggcgat cgcgccgggc gcgcccgtcc ctatccggaa
     6481 tccgttgtca gaaccttgac atagatgact tttccgtcac cgggggcgta aaaatcttcc
     6541 aggaaggccg tcggcgcgta accggaggcc tcgtagaagg cgcgggtcgg ggcgtactgg
     6601 ggccgggcgg aggtgtccgc atacacgcgc cgggcgccca gcgccgccat ccggctttcg
     6661 ctccgggcga gaagccgccc gccgatcccc tgccgctgcc gggaggcgtc cacgaccagc
     6721 cagtataggt cgaagctgtt ccgggtgccg ggaatcggtc cgtagcacgc gtaggccgag
     6781 atccgcccgc cctgcccggc gaccagaaaa tgatacccgc tgtcgccgcc cttccggagg
     6841 cgctcgtcca cgagctccaa ggcgatcgcg acctcttctt cggaaaagaa ccccgtcccg
     6901 tccacgaggc ggcggatggc ctcgcggtcc gcgggacgga ggtcggtgcg gaacctcagg
     6961 gcgggatcag tcacgggcgc cccctgtcga tctgaaggtt acggcgtcct gaaggatccg
     7021 cccgatcatc cggggatagg cgaatccggc ctgcccggcc gccgcgacga aaccgctgtc
     7081 cggggcgatc cccgggttgg cgttgatctc gagcacccag ggggcgcccg cggcgtcgac
     7141 gcggaagtcc atccgcgcgt agcccgcaag cgcgaagaga ttccagcagc gccgggcgat
     7201 gtccgtcagg gtctccagca acggcccatc cgcgggtgga aagtcgaagg agcgcggcga
     7261 gtgccgatat tcgaaagtgt cctcctccca tttcgcccgg tagccgacga tgcgaagctt
     7321 gtccgggggg tagtcgtcga agcggatctc cgcgggcggg aggacctccg gaccgttctt
     7381 tccggcgaga agtgagaggt tgaattcgcg gccgtcgatg taggcctcgg cgaaacagtc
     7441 gccgcccggc ccgtcgcccc gcgtccgcag ggtttcgtcc agatccttgg cgtttccggt
     7501 ggtgaagacc gcgtcgtcgt ccaggccgat ggaggcgtgc tcccagacgg acttgacgat
     7561 ccaccgtccc ggcggcatcg gctcggacgt tgcggcatcc cgcggcgtcc gccagggggg
     7621 ggtcttgaaa ccggcgtgcg ccagaagacg tttgccgagg agcttgttgg aggtgagaaa
     7681 gagggcctcc gtcgggttgc cggtgtaggg caggcggagt tggtccagca gggccggggc
     7741 cagatgaatg aggcgcccct ccccggtgag gggctcgtag aggttgaaga cgacgtgcgg
     7801 cgccagggcg cgcagccgtg cggcggtttc ttcgaggttc agggaaaagg gcagggcgac
     7861 cgccctgtgg ccgagggccc cgagcgcatc cagcacggcc tcccgctgga tgtcggtgtc
     7921 cgcctcgtcc cggctggctt ccgtccctac cggcgtatgc aggacaacca cgttcatgag
     7981 ggcgtcacac gatcgcgttt gcggacgggt ctttgcgaga ggaaaaggaa aaatggtgga
     8041 gatgaggggg ctcgaacccc tgacctcggc attgcgaacg ccgcgctctc ccagttgagc
     8101 tacatcccca cgccggcctt ccgtgcgaca ggcggtggac gtcgtaagaa cgcaacccgc
     8161 ctgtccaaaa gcgggcgaag catatacgat ccgccccgct cctgtcaagc aattcattgg
     8221 catcggcgtg ccgtcacgag cgcgccggac cgccggaatc ttgtcttgac aaatgctctg
     8281 gcgattccct atactcgccc atcgtttcac aaacggcagc atcatttcaa ccatccggga
     8341 ggggactcta tggccaagat cgacgccttt ttccagctca tgcatgaaca gggatcgtcc
     8401 gacctgcatc tggtgtccgg tcagcaaccg gccctgcgca tccgggggga gatcgaacgg
     8461 gtcaagtaca acgtgctcga aaacgacgaa ctcaaggcca tgctgtacga gatcgcgccg
     8521 gagcacaaga tcaagcagtt cgaagagacg ggagacatcg actttgccta cgaaatcccg
     8581 ggtctggccc gctaccgggc caacttcttc cagcagaagt acggggtggc ggccgtcttc
     8641 cgagagattc ccagcaagat cctgacctgc caggacctcg gcctgccgcc cgtcatcggc
     8701 aagctggcga gcttgccgcg ggggctcgtc ctcgtgacgg ggccgaccgg aagcggcaag
     8761 tcgacgacgc tcgccgcgat catcgacgag gccaaccgcc tccgcaagga ccacatcatc
     8821 acgatcgagg accccatcga gttcgtccac cagagccagg gctgtatcgt gaaccaccgc
     8881 gaggtgggcg tccacacgaa gtccttcacg gcggccctcc gcggcgccct ccgcgaggat
     8941 ccggacatca tcctggtcgg cgagctccgc gacttggaga cgatttcact tgccgtcgag
     9001 gcggcctcca ccggccatct cgtcttcggc accctccaca cgacgagcgc cgccaagacg
     9061 atcgaccggg tcatcgaggt cttcccctcc gaggaacaga tgcagatccg ctccaccctg
     9121 gccgacggca tccgggcggt catcgcccag gtcctcttca agcggatcga caagaagggc
     9181 cgctgcgccg ccctggagat cttgatcgcc aacgtggccg tgcggaacct gatccgtgag
     9241 tccaagacct tccagatccc gtcgatgatc cagacgggac gcaagtacgg gatgcagctt
     9301 ctggacgacg gcatcatgga tctgctcaac aaggggtgga tttccgcgga cgaggcctac
     9361 gccaagtgca acgacaaggc caaattccgg cccttcctga agacgccgcc cgcggacttc
     9421 acggaagttt agcgcaacgg gagggtaagc gatgagaaag caggaaatcg attacatctt
     9481 gggccggatg ctcgatacgt acggaaacgt gtcggatctg aacatcaccg tcggcaagcc
     9541 cttccaggtg gaaacctcag gccagttgag cggggtggaa ctcgatccgc cggtccccga
     9601 gctgacgccg ttccaaacgg agatttttgc actcaacctg atcaaccagg accgccgcct
     9661 gacggagacg ctcttggccg aggggtcctg cgactcgtcg tacgccctgc ccggcaaggc
     9721 ccggttccgc gtgaacatct tttcccagcg gggaaactac tccatcgtct taagaaagct
     9781 ggagacgcgg atcccgacgt tcgaggacat gaatttgccc gaggccttcc acaagatggc
     9841 cgaggagaag aacggcatca tcctggtcac gggcgccacg ggcagcggca aatcgacgtc
     9901 gctcgcggcg ctcctgaacg acatcaacga ccggaaatcc atccatatcg tcaccctcga
     9961 agacccggtg gaatacgtcc acccccaccg caaggcgacg ttcaaccagc gggagatggg
    10021 gacggatttc gacaccttcg ccaacggcct ccgggcggcc ctgcgccagg ccccgaaggt
    10081 catcctcgtc ggggaaatgc gcgaccggga gacggtggag atcggcctgt ccgccgcgga
    10141 gacggggcat ctcgtcctgt ccaccctcca caccgtcgac gcgggccaga cgattaaccg
    10201 catcctcggc atgttctcca acgaggagga gacccagatc cggatccgcc tggccgatac
    10261 ggtccgctgg atcgtctgcc agcggctcct gcccaaggag ggtggcggcc gcgtggcggc
    10321 cttcgagatc atggggtcga acctgcggac caaggatacg atcctccacg gcgagtcgga
    10381 ggggaagacc ttctacgaga tcatcgaggc cggcaaggcc ttcggctgga cgacctttga
    10441 cgattacatc atcaatctgt acgagcaggg gctgatcagc gaagagaccg gccgggccta
    10501 cgcctccaac aagggcatcg tcggccgtgg gatcgattcc gtgaagagct ccaagggaga
    10561 gaagaccacc gatctcggaa agctggaggt ggataagggc tacggcaagc cgaagccccg
    10621 cttctgagag ttcccccccc cctggccgga ttgatgccgt aactgcacgg tcaggacgcg
    10681 gatcgtaagc aaaggagaga gaggatggac gaagacaaga gtgcgggaca ggcagcggaa
    10741 aactacaatg cgtcggacaa gccgttcgat tttctcgagg ccggcggtgc cacggcgatc
    10801 gtctgcgagg ccgatccgga gaagcgggcc atgctgaatg cggcgctgaa atccctcgac
    10861 taccggatca cggaggccgc cgacgccaaa gacgccctga agcggatgcg cttccacatc
    10921 tacgacgtcg cggtgatcga cgagcgcttc gatgccgaaa atcccgatga caacgccgtg
    10981 ctcaagtacc tcgcggggct caacatgggc atccggcggc agacgttcgt ggccctggtg
    11041 acggaacgct tccggacgat ggacaacatg gcggccttca caaagagcgt caacgtgacg
    11101 atcaacgcaa ccaatctgga caacgcaacg acgatcatca aggagggcgt cgaggacaac
    11161 cgggccttct atcacatctt tcgtgaaagc atgaagaagg tggggcgggc ctgaaacgcc
    11221 gcgttcacgc atctgtcgat caccggcccg tgaggttgtc ccgcgggcct tttttgttta
    11281 tgcggacccg cagctcggcg tcgcgggcct gttcgtccat gcccagggca cgacaggccg
    11341 tggcgagccg ttcgtagagg gccggggtgt ccggtgccgc ggcgacggcc gcccggaagg
    11401 cctccagggc ctggcgatgc cggccgtcac gggtcagggc gttgcccagg cggttgtaat
    11461 aggccccggc gtcttccggt ctcagggcga cggcggtatc gtagcagccg cgggcttctt
    11521 cgagtcggcc ccggcggaca aggatgtcgc ccagggtgca gtgatagagg ggctcgtcgg
    11581 gccgggatgc caggcagtgg cgcgcggcgg tttcggcccg ttcggtttcg ccggccttga
    11641 ggtaggcgtc ggccaggcgc aggaaaaaca cggcgccggt gtcgggctcg agggcggcgg
    11701 cccgtccgta cgccgtctcc gcctccgtaa acttccccgc ggcgatcagg gcgttgccaa
    11761 ggcggcagaa ggccatcgcg tcgtccggtt ccatctccgc catcttccgg aacgcggcgg
    11821 cggcctccgc cggttgcgcc gccagcaggc aggcctcacc cagttcggcc agcagggcgt
    11881 aatcctcggg ctgggccgcg caggcccgcc cgcagacttc gaccaggcgg tcgtgcagcc
    11941 cgtgctcctt gtagatccgc accagttcgt cgaagccgaa tcccgtgaag accttccggc
    12001 ccagttcccg gtccaggagg cgctcgaagc aatcgatcgc ctcctcccag gcgccttggt
    12061 catggcaggc ccaggccagg gcgagcaggt aggactcctc gcccgggtgg gcggcgacga
    12121 gcgtgcggta cagggcgacg gcatcctcga agcggccgtc ccgcatgcat ttttccgcct
    12181 ccaggcgcat ctgttcgtct tcgtaggtcg gctggctcat gccgcgtgct ccttcaagtg
    12241 tccgccctcc cccaagcggc cccgccgcgc cgcttcccgc cggtccggaa ggacgcgctt
    12301 cgcacgggtc ggggcggtgt tcaatccgtg aaggggcatc gggggcgggg aagtccggag
    12361 aaaatgaatc gggggagggc tgcggggaat tccccagacc ctcccccttc gggattcagc
    12421 aactgcagga tgtgctgcac gacgttccgc aaccgccgga cgcggcgatg ttggaactca
    12481 gtttgaaacc ggaacccatc ggagaggtga tgaagtccac ggtgatcggt ttggcctgct
    12541 cccagagggc cttttcgatg atgaaggtca ggccgtcgtt cttgaaaacc tcgtcgtttt
    12601 cgtttggctc atccagagcc attcccaggg agggtccgga tcacccgccg cccatcatcg
    12661 tgatgcggac ggaggccggc ccctgccggt tcttcatgaa atccttgatc atttcgtttg
    12721 ccttctcggt gacttccagc atatgcgctc ctttgaatgt tttccttgcg tcagcggttc
    12781 aaatcgcgtt tctctctcaa ggtcggccta agctatacat taaaaaaact ttgtcaatcg
    12841 aattttttcg ggacgggggc tggcggccgc cgtcgatttg tgcttgaagt cttgtcggag
    12901 gcgggatatg caaagcgaac ttcccgcttc agacaagccg tctccgtgtt cgggccccct
    12961 gggggccggg gagcgacgat ggatttcgag atccgtccct gttcagaaac cgatgccggg
    13021 attttggccg agacgatccg ggccgccttt cgcgacgtcg cggagcgctt cggcctgacc
    13081 cgggagaacg ccccgcggca cccgtccaac tgttcggcgg actgggtcga aaaggacatg
    13141 gcgcgcgggg tcgtctatta catgctggag gacagggggc gcgcttccgg ttgcgtcgcg
    13201 ctcgaaatgg ccaacgccga ggtcggctat ctggagcgcc tggccgtgct tcccgagcgg
    13261 cgccggcgcg gtttggggcg ggtcctcgtc gcgtacgcgc tttcggaggc cgcccggcgc
    13321 ggcgcccggc gcgtcaacat cggcatgatt gcggagcagg ccgaactgaa ggactggtac
    13381 cgggggctgg ggttcgtcga gggggagacc cgggaattcg cccacctgcc gttccgcgtg
    13441 acctttctgt cgcaggggat tcaaggagaa gaaccatgag cagccacatt catcacgtgc
    13501 acatctttgc gtccaatctc gatcgaacga tcgaattcta ccgggacttc ttcgggggca
    13561 cggtggtcct ggacatggag ctggccgggg cgcgaaacgt cttcatgcgg gtcgggaccg
    13621 gccgcattca tttttacgat cagccccccc gattctcggg ccggggaagc atccaccatt
    13681 tcgggatcca gaccgacgac gtcgaggccg tcgtgaaccg gatgaaggcg aagggcgtgg
    13741 tcttcaccaa gggcatcacg gatgccgggt tctggaaata catcatggtg ccggcgccgg
    13801 acgacatcct catcgaggtg ttccaggtgg accgggcgca gatccccgcc gacctgctcg
    13861 gttatttcga gtggaacgat ccccggaagg agttggaggc atgagcgatt acggaaccta
    13921 tctgaaacga cttcaggcgg gggagctgca cctcaacccc ttcctcgcct tcctcgacat
    13981 gcggcccgag gcgatggacg acgggtacgc ccgcttccgg atgccgatcc ggccggagta
    14041 cctgcagggt gcgggcaaca tgcagggggg gatgatcgtg gcgatggccg acgaggccgc
    14101 cgcccacgcg atcatgaccc tgctcaagcc ccgggagggc ctgaccacga tcgaactgaa
    14161 gagcaatttc ctggcgccgg tacgggcggg cgacctggtt gccgaggccc gggtcttcaa
    14221 gaagggccgc acgctcatga tcgccgactg tacggtgacc gacgaccgcg agcgcccggt
    14281 cctccgcctg tcggcgacgt tcctgatcat ccgggagtaa ccggcgtcgc cgggaacgcg
    14341 cagtccgtcg gccggctcgc ccggttattc tatctttcca tccatccctt gccgggccat
    14401 gacatggaac ggtttatatt gacagccaat attgatttgt gtatatcaaa atcgcatccc
    14461 gttatataca agccggctca tgtataatct gttggaggca aaatgcagcc ctttgaaccg
    14521 caacagcttc cgatcgatga catcccgtgg gacgccctga tccccctgat cggcagggca
    14581 aatcgctccc tggcttatta tgacgggatt ctgtacggcc tcgccaatcc cgatgtcctc
    14641 ctttcccccc tcacgacgcg ggaagccgtg ttatcctcta agatcgaagg cacgcaggcc
    14701 accctgggtg aggtcttgaa attcgaagcc ggggcggcgc ctgccgagga atcccgtgtc
    14761 cgggatatcg aagagatcat gaattatcgg cgcgccatgc gccgggccga agaggaactg
    14821 aaggtccgtc ccttttcctt gaatcttctg aaagagctgc acgcggtgct gctggacggt
    14881 gtccggggaa gattcgaggc caggggacaa ttccgctcct cacaaaactg gatcggaatt
    14941 cccggaacac ccatcgagga ggcgcaattt gtccctcccg atcccgcaaa gatgatcgaa
    15001 catctgtatg gctgggagaa atattatcat ttcgatcgtc cggatccctt ggtccagttg
    15061 gcgatcattc acgcccagtt cgagttgatt cacccttttc tggacggcaa cggccgtctc
    15121 ggaaggatga tcgttccgct ctttctcttt gaaagaaggc tgatttccag tcccgtgttt
    15181 tatatatccg cctacctgga agctcaccgg gacgcctatg cagccggact ccggtcgttg
    15241 agcgataaaa cgccggcctc ctgggcccat tggatcggtt tttttctccg tgccatggac
    15301 gaacaggcga aggaaaatgc aaagaaggcc caggccatca tcgacttgta cggccgcctg
    15361 aaagaaagga tgatcgactt gacccactcc caatacgccg taccgcttct ggatcatttg
    15421 ttcgatcaac cggtttttac cagctcgctt ttcgatgaac gttccgggat gcccagcaag
    15481 ccgatggtga tgaatatgct ggggcgactg aagaaggcgg ggatcctcaa ggtggtccgg
    15541 gaaggcagtg gccgccgacc gcaaatcctg gcattggccg aactgatcaa tgtctgtgaa
    15601 ggggcggatg tcatctgatt catcctggtg cccttcgaac tgggcaagca gcaggagtac
    15661 ataggcaacc cgctggtcgt cgcccaagag cacaatgacg aggctgactt ctacctctcc
    15721 cacgggacgg cgatggagat tcacggcatg gtcatgcaac cgcgccttgt tttccacatc
    15781 acggcgctga aggagtcggg tgacagtcaa ggtccgcttc agccgatacg accgacactc
    15841 atatgaaaac agtcatacat tctgcctcgt ctacgaaggg cctctctctc ctaggcctca
    15901 gactgccgac cttccagaat ttgttgaaat gtacgggaaa ttggccaggg agtcgagaag
    15961 ggtttatttc aattgatgaa ttcacgcata aaatgctaac caatggcata aatgacctat
    16021 aatcaaggtg attgattatg gcaaaggcga ttttcacaac aaagatcact ccaagttata
    16081 atgaccttcc ggaattgatg taccattttc caaaaaccta tttgcgccaa gcaaacctga
    16141 ctgttggtga ttggatcata tattacgaac cgcgcagaga agacatttct ccggctggtc
    16201 atgcggggcg tcaatgctat tttgcaacag cacgagtaac ccacatcaaa taagatattg
    16261 ctgtgaagga ccattattat gctttcgtag aaaattacgt tgaattttcg gcgcccgttc
    16321 ctttcaaaat tggctcattc tattacgaaa gcgggctcaa gaaagccgat ggttcaacca
    16381 acaaaggggc gtttgggaga gccattagaa ttatccaaga cgatgagtat caggccattg
    16441 ttgcggcagg catggcacag attaacgagc agaccgtacg agacg
//
LOCUS       DAOOLJ010000036        15780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3937_length_15780_cov_16.773227, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000036 DAOOLJ010000000
VERSION     DAOOLJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_15780_cov_16.773227"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLW16_06960"
     CDS             <1..489
                     /locus_tag="PLW16_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPB70681.1"
                     /translation="PAFLCRQTDFVMEVARRAGVINLKKGQFLAPSDCAPMVEKARAA
                     GNERIILTERGTSFGYHNLVADFRALPILRSLGYPVVFDATHSVQLPGGSGNASGGDR
                     DMVPYLCRAAVAAGVDGLFLEVYPDPSQARCDGPNSLALDTLPGLLKTLIAIDRLVRE
                     TF"
     gene            658..1200
                     /locus_tag="PLW16_06965"
     CDS             658..1200
                     /locus_tag="PLW16_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPB70682.1"
                     /translation="MTDADREKVLALAAKIRLLILDVDGVLTDGRIIMDDEGRESKHF
                     DVKDGHGLKMLMRGGVDVVIITGRLSRVVEHRARDLGIQEVHQQSFDKLAVFEELLPR
                     RGVSAAEVAYVGDDIVDIPLLKRVGLAVGVADAAEEVLRTVDYVTLKPGGRGAVREVC
                     ELILQARGLWPGIGESYAFA"
     gene            1267..1839
                     /gene="lptC"
                     /locus_tag="PLW16_06970"
     CDS             1267..1839
                     /gene="lptC"
                     /locus_tag="PLW16_06970"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPB70683.1"
                     /translation="MAMPIGGILKRKRLALAGAAVAIVCVGAVVLIHSFSRIPEQVQL
                     QIRPGSADLEIRDVRFQEVSDDASRWEVQADLAKYMKKDNLALFEHVRIRLLRTDGDT
                     YLLTGDRARLRTDTRDLELTGHVTLVTHRGETVRTDRLRYDHAGRKILTDDPVTLEGR
                     GMRVQGVGMKISLADRDLRLFSKVKAEVSP"
     gene            1836..2402
                     /gene="lptA"
                     /locus_tag="PLW16_06975"
     CDS             1836..2402
                     /gene="lptA"
                     /locus_tag="PLW16_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HPB70684.1"
                     /translation="MKKILSLLLVWMVLSPLSLAAEEPAAKKGIRVDSNQPIRIVSDR
                     LEAFSEKRMVVFSGNAVAVQGDRTIHADRLILFYRKADRDPDRPEKVEKGGEEKGLGD
                     LERVEAEGHVRVIQGARVVTGEEAVFYQEEQRVVMTGNAVMREGENVIRGQRIVVFLN
                     ENRGVVEADPNKRVTATIYPSEKKDRRK"
     gene            2407..3183
                     /gene="lptB"
                     /locus_tag="PLW16_06980"
     CDS             2407..3183
                     /gene="lptB"
                     /locus_tag="PLW16_06980"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPB70685.1"
                     /translation="MGALTAERLTKIYGGRRVVNGVDLTIRPGEVVGLLGPNGAGKTT
                     TFYMIVGLIKPDDGRILLGAEDISACPMYLRARKGLNYLPQEPSIFRKLTVEENILAI
                     LEILDIDRRERLRRLRELLAELDLTPLARSPAYALSGGERRRVEITRALVTSPKFILL
                     DEPFAGIDPLAVADIQKIIFKLKGKGIGVIISDHNVRETLTVCDRAYIVNEGAILVEG
                     DPDSIARSEIARKIYFGEDFSFSADVPRKATGETPANEIE"
     gene            3183..4649
                     /gene="rpoN"
                     /locus_tag="PLW16_06985"
     CDS             3183..4649
                     /gene="rpoN"
                     /locus_tag="PLW16_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPB70686.1"
                     /translation="MAFELRQNLKLSQQLVMTPQLQQAIKLLQLSRLELVDQINQELE
                     ENPLLEEVASDEYPDEGGGGQDVDETRLHDAEETKPIAHTEELTGEGDGKSEFDWDNY
                     LEDYGPMGVTYDRSDAETHTWDNLLSSKPNLTDHLMWQLKLSPLTPTEMRIGEQIIGN
                     IDPNGYLAATLGEIAEQEKVDEPAVEAVLGKVQEFDPPGIAARNLGECLLLQARMTGV
                     GTPLIERIITGHLRDLEMKNYGQVARKLKVDLDDVIQAVQIIRNMDPKPGSPFSEDRT
                     QPITPDVFVVKSGDDYKVILNDEGLPRLRISNFYREVMAGQGNRPDAEIGKRYIKDRV
                     QSATWLIKSIQQRQRTILKVTESIVRFQRAFFDNGVNFMKPLILRDVADDVEMHESTI
                     SRVVTNKYMHSPRGIFELKYFFGSSLYRTTGEAVASKSVKEDIRMLIAAENPKKPLSD
                     SEIAKRLSARGIGIARRTVAKYREMMGILPSSRRKNLF"
     gene            4768..5304
                     /gene="raiA"
                     /locus_tag="PLW16_06990"
     CDS             4768..5304
                     /gene="raiA"
                     /locus_tag="PLW16_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPB70687.1"
                     /translation="MKIAVTFRNAEGETWQKEYVEEKLSKLKKYIDGSVEAHIVLSIE
                     KFRNVAEISLAADGLNINGREEAKDMRLAIDGVLDKIERQLKKRKEKNRGHKGAAARE
                     EAAAGAALLDEAEDATAAGLMETRKMVLEPMSLDDAVMRLESARNPFIVYRDVLSEHV
                     HIVFRRDDGRLVLIETNA"
     gene            5321..5782
                     /locus_tag="PLW16_06995"
     CDS             5321..5782
                     /locus_tag="PLW16_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB70688.1"
                     /translation="MKITEMLRKEYMIAKLGARGKTAVLEEMVAQFHRGPETFSEPEM
                     VRVLLEREKLGSTGIGEGIAIPHGKLEGLEGMIVAFGRSPEGIDFEAMDGKPVHLFFM
                     LMAPENSTGQHLKVLARISRMLKDGDFRQQLLDAGSVDQLYRIIADKDDRC"
     gene            5862..6740
                     /locus_tag="PLW16_07000"
     CDS             5862..6740
                     /locus_tag="PLW16_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70689.1"
                     /translation="MSVAGTGGFDRVVVPASSLEPFRILRFDEAGPEGGVPGLVILPR
                     GDLRRLMPRTSVGARSALAIFRKNPTRVILCAETRKIPASLKAFVERHGMIAAASRFD
                     PHLLQSRLAALVRERLERTVVYQGGLIEIDGRGLLLVGDSGCGKTTCALCLAKRGAWW
                     IADDRVEIVRLRHRLLGRAPRSIHRLVALKTQGVADVTQFVDPQTIKASSDIAGVVTL
                     VSPREGAHRKTRGGERETILGIDLPRVTISVPARGGACGWVVLSRGAPSAFAFLVHGP
                     CPLGNARSEMQGGGSS"
     gene            6737..7603
                     /gene="rapZ"
                     /locus_tag="PLW16_07005"
     CDS             6737..7603
                     /gene="rapZ"
                     /locus_tag="PLW16_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPB70690.1"
                     /translation="MKHLRVVIISGLSGSGKSTALKALEDIGFYCVDNMPVVLLPKFL
                     KIQSDPAKQIDQVAMVMDLREKSFLEKYPRIFNRLRQQGYRIEVIFIDANDEILLHRF
                     SETRRVHPVSVKGSVMDGIRLEREMLFPLKEMADRVIDTSSLNVHQLKDAVQRAFLAS
                     SDKKRLIVNVVSFGYRYGLPADADVVFDVRFLPNPHFVDELKRHDGHHPSVRNYVMAT
                     DEAKGFIQRLFDLMDFIVPLYEREGKARINIALGCTGGHHRSVVMANQLGGYFSEKDY
                     LVNITHRDITKS"
     gene            complement(7600..8937)
                     /locus_tag="PLW16_07010"
     CDS             complement(7600..8937)
                     /locus_tag="PLW16_07010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPB70691.1"
                     /translation="MRKKSSFFWISLICAIVVVAGGLFWFFSTAGEWQKPEIRLSEDL
                     RSLGQRGVLTLELRDNRGLSAASVQITQGSQTLPVASVRLDDKPLRQRTVNVPIDPLA
                     LKLREGSAVLTVSASDRSLWNNTTVVTRPVTIELSPPQIFPNNPVNIFFPGGAGVVTY
                     RLSKPAVQTGVQVDEAFFPAHPVLIAGRASYICYFAIPMDAQERLVDMRITARDAAGN
                     AASSAIPRTMKSRKFRSDKMAVGDAFLQQKMPEFQSAIPALRGKTAIEVFRYVNGTLR
                     LENEQTIRDLCRKTQPRALWEGPFLRMKNAAPMALFGDRRTWEYQGQVVGQSLHLGED
                     LASLNNSAIEAANNGIVVFTGPLGIYGNAVLIDHGLGLFSLYGHLSAIDARVGQAVQR
                     EDVIGRSGTTGLAGGDHLHFSILVGGQFVDPREWWDPHWIEDNVLKKLQAGSL"
     gene            9128..10330
                     /gene="coaBC"
                     /locus_tag="PLW16_07015"
     CDS             9128..10330
                     /gene="coaBC"
                     /locus_tag="PLW16_07015"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPB70692.1"
                     /translation="MWKGKNVVLGITGGIAAYKAAELTREIVRREAAVRVVMTRHAQE
                     FIAPLTLETLSGNRVCTDLFTPEAPHEIDHIALARWADVLIVAPATANLIGKAAGGIA
                     DDLLTTTLLATRAPVLLCPAMNDAMWENPIVGENLDRLRHRGCAVVEPARGSLACGTE
                     GAGRLADLGDLLDAAEALFTPQDFSGETVLVTAGPTREPLDPVRFITNYSSGKMGYAI
                     AHAARMRGATVILVSGPTVLVPPGGVELVQVQSAREMRDAVLGRLDETTVIVKAAAVA
                     DYRPTVCEDQKIKKRPGDLRLCLERNPDIIAEIGRKKDGRILVGFAMESENLLENART
                     KMRAKKMDLIVANDLREAGAGFQGDTNVIRILSPDGRVEALPLMGKREAADRILDRIR
                     DLRGRSGR"
     gene            10335..11141
                     /locus_tag="PLW16_07020"
     CDS             10335..11141
                     /locus_tag="PLW16_07020"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB70693.1"
                     /translation="MREAFQELVHHLHARLREDQARGGRLSYLVAAGDSGVGREAAPF
                     PETASRKTPPRDRTVPVRAAGETLEAIRQALGDCQRCPLGAKRRRLVFGEGDPHAELV
                     FIGEAPGADEDLQGRPFVGRAGQLLTKIIEAMGLKRDDVYICNILKCRPPGNRNPAPD
                     EVAACEPFLIRQLQAIRPRFICALGSVAARTLLKTEAPITVLRGRFQSYQGIRLMPTY
                     HPAYLLRNPVAKKKVWEDVQIIMKALDLPIPERKNGLPGRGAPAGDAEGI"
     gene            11138..12463
                     /locus_tag="PLW16_07025"
     CDS             11138..12463
                     /locus_tag="PLW16_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPB70694.1"
                     /translation="MMGPVEQRHLPLIVVVMLVLLSATADAVPGRSPTFTVIRVDAAI
                     TPPVAQYILQSLEEATRTRSDGLVIRLDTPGGLDLAMRDIVKGILGADIPVIVYVSPA
                     GARAASAGMMITVAAHVAAMTPGTNIGAAHPVGIGLGGGMDKIMQAKVENDAVAYIRG
                     IAKGRGRNPDWVERAVRKSASVSAEEALKLGVIDEVAPDLAHLLARIDGKVVTLASGK
                     RVLKTRGAVTVEKKMGVRQGILTVLADPNIAYILLLIGLAGLYFEFSHPGAILPGVMG
                     GISLILAFFALQTLPVNYAGVLLILFGITLFIAEVKVMSHGILTVGGIVSLVMGSLIL
                     FDSPEPALRVSFQVMIPTLIVICLFFVAVISLVVKAHRGRRFTGMEGMLGLEGKAVNA
                     VGPDAGTVLVKGEYWRARSDEPIAPGLKIRVIRVDGLRLLVGEIHPGEP"
     gene            12594..13343
                     /locus_tag="PLW16_07030"
     CDS             12594..13343
                     /locus_tag="PLW16_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPB70695.1"
                     /translation="MSVTVIVIVVLVVMFLASAIRILNEYERGVIFRLGRIIDQKGPG
                     LIILIPVIDKMVKVDMRTITLDVPPQDVITRDNVSIKVNAVVYFRVMDANSAVIDVEN
                     FLYATSQLAQTTLRSVCGQVELDEILSQREKINLHLQEILDRSTDSWGIKVSLVEVKQ
                     IDLPEEMKRAIAKQAEAERERRAKVINAEGEFQAAQKLIEAAALMETQPMSLQLRYLQ
                     TLNQIASENNSTTLFPIPIDLFKPFVKLAEK"
     gene            13396..13713
                     /locus_tag="PLW16_07035"
     CDS             13396..13713
                     /locus_tag="PLW16_07035"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPB70696.1"
                     /translation="MHGKDKRHLLTGGLVLIALGVLIILDKTGIYTFSHSWPILLIVI
                     SLGVLLQRVKDLGGWIIGASGVVFLITENWGYRLFEIAKYLLPIMLIIIGADLIRRRA
                     RKS"
     gene            13781..14515
                     /locus_tag="PLW16_07040"
     CDS             13781..14515
                     /locus_tag="PLW16_07040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HPB70697.1"
                     /translation="MNRVLLMSDFDGTICSVDVGDGLGARFAAPEWRDIDRAYNEGRI
                     GSRLAYPRIAALMRGLTRDYLLAFALERARLDPGFPAFVSFCRERGYDFMIVSDGLDV
                     YIRAILEREGLADIEFYANAVSFPEADRIAFAFPHANVRCGKCGTCKTGLLARQRRRH
                     DLILYVGDGHSDVCPAQHADGVFAKGILREKCLLNGTPFRSYRNFTDIQRILAAAEPG
                     ALGSIWKDGLEEGPEKGERHVSGGLI"
     gene            14493..14861
                     /locus_tag="PLW16_07045"
     CDS             14493..14861
                     /locus_tag="PLW16_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB70698.1"
                     /translation="MFQVASYETYSDGKVIFTEGSHGDWIYVVEEGAVEISKKDGEQK
                     VVIEVLKPGDLFGELAYIAKIARTATATAVGKTVIGILDRDYFDREFNKLPGDFQVMF
                     KTVALRLKATTEKVTAAYRG"
     gene            complement(14937..>15780)
                     /locus_tag="PLW16_07050"
     CDS             complement(14937..>15780)
                     /locus_tag="PLW16_07050"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HPB70699.1"
                     /translation="ALRTLRALGVNRLTLGCQSFDDAVLAFLGRRHSAGQCRDAILAV
                     RAAGFDDLGLDLIYGLPAPGGDPGTAFRGWLASLQTALSFQPEHLSCYQLTLEADTPL
                     AATLALSPLSLPDDDLSARYFLTTSERLEAAGYRHYEVSNFACDDDHRARHNRKYWDH
                     TPYLGLGPSAHSFDGTRRWWNPRDFDAWVGALRDGMRPIAGEEILTLDQLRLEALFLG
                     LRTREGIDMEAYRKRYGFDLCAERGDKIAQLAAAGLVRADGYRFSPTRRGLVCADFLA
                     RFLS"
BASE COUNT         3079 a         4727 c         5190 g         2784 t
ORIGIN      
        1 ccggcctttc tctgccgcca gacggacttt gtcatggagg tggcgcgtcg cgcgggcgtc
       61 atcaacctca agaagggcca gtttctcgcc ccctcggact gtgcccccat ggtcgaaaag
      121 gcccgggcgg cgggcaacga gcgcatcatt ctgacggaac gcggaaccag cttcgggtac
      181 cataacctgg tggccgactt ccgcgcgctg cccattctcc ggtccctggg ctatcccgtg
      241 gtctttgacg ccacgcacag cgtccagctt cccgggggga gcggaaacgc ctcgggcggc
      301 gatcgcgaca tggtgcccta cctctgccgg gcggcggtgg cggcgggggt ggacggactg
      361 tttctcgagg tgtatccgga cccctctcag gcccggtgcg atgggcccaa ttccctggcg
      421 ctcgacacgc tgcccggact cctcaagacc ctcatcgcga tcgatcgcct cgtcagggaa
      481 accttttgat tgacgcggac cgtccgctgc gcgcaccgct ggcgtgcgtc gagggacggg
      541 aaagcctccg acacgccccc gggggcggct cgccccgggc gtgcggttga agggcagggg
      601 aaaatccttt aagagaaagg tcccaaagcc aaaacggact aggacgcgag gtgaacgatg
      661 acggatgcgg acagggagaa ggtgttggcg ctggcggcga aaatccgctt gctgattctc
      721 gatgtggacg gcgtgttgac ggatgggcgg atcatcatgg acgacgaagg gcgtgagagc
      781 aagcacttcg atgtcaagga cggtcacgga ctgaagatgc tgatgcgcgg cggtgtcgat
      841 gtcgtcatca tcacgggacg cctgtcccgg gtggtcgagc accgcgcccg ggacctggga
      901 atccaggagg tgcatcagca gagcttcgac aaactcgccg tcttcgagga actgcttccc
      961 cgtcgcggcg tgtcggctgc ggaggtggct tatgtggggg acgacatcgt cgatattccc
     1021 ctgctgaagc gtgtcggact ggcggtcggc gtggcggatg ccgcggagga agttcttcga
     1081 acagtcgatt acgtcaccct gaagccgggg ggccgggggg cggtccggga ggtgtgcgaa
     1141 ctcatcctcc aggcccgcgg gctctggcca ggtatcggcg agagctacgc gtttgcctga
     1201 ggtccgattc gtctttacag gcctccgggc gaatgctata gaacacccag ggggtgcgcg
     1261 gcgctcatgg ctatgccgat cggagggatc cttaaaagaa aacgcctcgc gcttgccggg
     1321 gcggccgtcg cgatcgtgtg tgtgggcgcg gtcgtcctga tccactcctt ttccagaatc
     1381 ccggagcagg tacagcttca gatcagaccc ggcagtgcgg atctggaaat ccgggacgtc
     1441 cgctttcagg aagtaagcga cgacgcctcc aggtgggagg tccaagccga tctggcgaaa
     1501 tatatgaaga aggataacct ggcccttttc gagcatgtcc gaatccggtt gctccgcacg
     1561 gacggcgaca cctacctcct gacgggagac cgggcccggt tgcgtaccga cacccgggat
     1621 ctggagctga ccgggcatgt gacgctggtg acccaccgag gagagaccgt acgaacggat
     1681 cggctgcggt acgatcacgc cggacgcaag attttaacag acgaccccgt caccctggag
     1741 gggcggggga tgcgtgtgca gggggtggga atgaagattt ctctggccga tcgggacctg
     1801 aggctgttct cgaaggtgaa ggccgaggta tccccgtgaa gaagatcctc tcgcttcttc
     1861 tggtctggat ggtcttgtcg cccttgtccc tcgcggcgga agaacccgcg gcgaagaagg
     1921 ggatccgggt cgactcgaac cagcccatcc ggatcgtctc cgaccgcctg gaagccttca
     1981 gcgaaaagag gatggtggtt ttctccggaa atgccgtggc cgttcaggga gaccggacga
     2041 tccacgccga ccggctcatc ctgttttacc gcaaggccga ccgggacccg gaccgcccgg
     2101 aaaaagtcga aaaaggcgga gaggagaaag gtctgggcga tctcgagcgg gtggaggccg
     2161 agggccatgt ccgtgtgatc cagggcgccc gggtcgtgac gggggaggag gccgtttttt
     2221 atcaggagga gcagcgggtg gtcatgacgg gaaacgccgt catgcgggaa ggagaaaatg
     2281 tcatccgggg tcagagaatc gtcgtcttcc tgaacgagaa tcgcggggtc gtcgaagcag
     2341 acccgaacaa gcgggtgacg gcgaccattt atccctccga aaaaaaggac aggcggaaat
     2401 gaagtaatgg gggccctgac cgcggaaaga ctcacgaaga tttacggggg acgccgggtg
     2461 gtaaacggcg tcgatctgac gatccggccc ggcgaggtgg tcggcctgct ggggccgaac
     2521 ggtgccggca agacgacgac attctatatg atcgtcggcc tcatcaagcc cgatgacggc
     2581 cgtatccttc tcggggcaga ggacatcagc gcctgtccga tgtacctccg ggcgcgcaag
     2641 gggctgaact atctcccgca ggaaccctcc atcttccgaa aactgaccgt cgaggagaac
     2701 attctggcca tcctggaaat tctggacatc gaccgtcggg agcgcctccg ccggctacgc
     2761 gaactgctcg ccgaactgga cctgacgccc ttggcacgca gtcccgcgta tgccctgtcg
     2821 gggggcgagc ggcgccgggt ggagattacc cgtgccctgg tgacctctcc aaagttcatc
     2881 ctcctggacg agcccttcgc cgggatcgat cccctggccg ttgcggacat ccagaagatc
     2941 atctttaagt tgaaaggaaa ggggatcggg gttatcattt ccgaccacaa cgtgcgagag
     3001 accctgacgg tgtgcgaccg cgcctacatc gtcaacgagg gcgccattct cgtggagggc
     3061 gatccggatt cgatcgcccg gagcgagatt gcccgcaaga tctatttcgg tgaggatttc
     3121 agcttctccg ccgatgtccc ccgaaaagcg accggcgaaa cccctgcaaa cgaaattgag
     3181 tgatggcgtt tgaattaaga cagaacctga agctctccca gcagctcgtc atgacgcctc
     3241 aactgcagca ggcgatcaaa ctgctgcaac tgtcacgtct ggaactcgtc gatcagatca
     3301 atcaggagct ggaggaaaac cccctgctgg aagaggtggc gtccgacgag tacccggatg
     3361 aagggggggg cggccaggat gtcgacgaga cccgtctcca cgacgcggaa gagactaagc
     3421 cgatcgccca tacggaggag ttgaccggcg agggtgacgg caagtcggag ttcgattggg
     3481 acaactatct cgaagactac ggtccgatgg gggttaccta tgaccggagc gacgccgaaa
     3541 cacacacctg ggacaacctg ctgtcatcca agccgaacct gaccgatcac ctgatgtggc
     3601 agttgaagct gtcgccgctg accccgaccg aaatgcggat cggagagcag atcatcggca
     3661 acatcgatcc aaacgggtat ctggcggcga cactcgggga gatcgcagaa caggagaagg
     3721 tggacgaacc ggcggtcgag gcggttctcg gaaaggtcca ggagttcgat cccccgggta
     3781 tcgccgcccg taatctgggg gaatgtctcc tgctccaggc gcgcatgacc ggtgtcggaa
     3841 cgccgttgat cgaacggatc atcaccggtc atctccgcga tctggagatg aagaactacg
     3901 gccaggtcgc gcggaagctc aaggtcgacc tcgatgacgt catacaggcc gtccaaatca
     3961 tccgcaacat ggatcccaag cccggaagcc ccttcagcga ggaccggacg caaccgatca
     4021 ccccggatgt tttcgtggtc aagtcgggtg acgattacaa ggtaatcctg aatgatgaag
     4081 gactgccgcg gctgcggatc agcaatttct accgcgaagt catggccggg caggggaatc
     4141 gccccgacgc cgagatcggg aagcgttaca tcaaggaccg cgttcaatcc gccacctggc
     4201 tcatcaagag cattcagcag cgccagcgga cgatcctgaa ggtgacggaa agcatcgtcc
     4261 gattccagcg ggcgtttttc gacaacgggg tcaatttcat gaagcccctg atcctgaggg
     4321 acgtggccga cgacgtggag atgcacgaat ccaccatcag ccgcgtcgtc accaacaaat
     4381 acatgcattc ccctcgcggt attttcgaac tcaagtactt cttcggcagc agcctctacc
     4441 gcaccaccgg ggaggccgtt gcctccaaga gcgtcaagga agatatccgc atgctcatcg
     4501 ccgctgaaaa ccccaagaaa ccgctgagcg acagcgagat cgccaagcgc ctgagtgcac
     4561 gtgggattgg gattgcccgg agaacggtcg ccaagtaccg ggaaatgatg ggcattcttc
     4621 cctcctcccg gcggaagaac ctgttctgac tgcaaaaagg ggttgacatt tagcggcaac
     4681 ccctctatac ggctcccctt tatgaagccg ggacgcggtt gaaccgacgg gatttcgcgg
     4741 ccggccatcc ccaggaggtt tgagatcatg aaaattgccg ttacgttcag gaatgccgaa
     4801 ggcgagacgt ggcagaaaga atatgttgaa gaaaaactgt cgaagttgaa aaaatacatc
     4861 gacggatccg tcgaggcgca catcgtcctg tccatcgaga aattcaggaa tgttgcggaa
     4921 atcagcctgg ccgccgacgg cttgaacatc aacggacggg aggaggccaa ggacatgcgc
     4981 ctggccatcg acggcgtcct agacaagatc gaacggcagc tcaagaaacg caaggagaag
     5041 aatcggggtc acaagggcgc ggcggcccgt gaggaagccg ccgctggagc ggcactgctc
     5101 gacgaggcgg aggatgcgac cgccgccggc ctcatggaaa cgcgcaagat ggtcctggag
     5161 cccatgtcgc tcgacgacgc ggtcatgcgc ctcgaatcgg cgcgaaaccc ctttatcgtt
     5221 taccgcgatg ttctgtcgga gcatgtccat atcgttttca gacgcgacga cggtcggctg
     5281 gtcctgatcg aaacgaacgc ctgaggggtg gtgggcagac atgaaaatca cggagatgct
     5341 ccgcaaagag tatatgatcg caaagctagg cgcccgcggg aagacggccg tcctcgagga
     5401 aatggtcgcg cagtttcacc gcggtccgga aacgttcagc gagccggaga tggtccgcgt
     5461 gctcctggag cgggagaaac tcgggagtac gggcatcgga gagggaatcg ccattccgca
     5521 cggcaagctg gaaggtctgg aaggcatgat cgttgccttc ggacgaagcc cggaagggat
     5581 cgacttcgaa gcgatggatg ggaagcccgt acatctcttc tttatgctca tggcgcccga
     5641 aaattccacc ggacagcacc tgaaagtcct ggccaggatt tcccgcatgc tgaaggacgg
     5701 cgattttcgg caacaactgc tggatgccgg atcggtcgat cagctctaca ggattatcgc
     5761 cgacaaggac gaccgctgct gacgctcggc gggggacgat ggtccgtcgc ggcatgacgc
     5821 cgagggcgct tctcgttgac gggggggagc gtctgggaat cgtgagtgtt gccggtaccg
     5881 gcggattcga ccgcgttgtt gtgccggcgt cttctctgga gcccttccgg atcctgcgtt
     5941 tcgatgaggc cgggccggag gggggtgttc cgggcctggt tatcctcccg cgcggggacc
     6001 tgcggcgcct gatgccgcgg acgtcggtgg gagcgcggtc cgcgctcgcg atcttccgta
     6061 agaatcccac ccgcgttatc ctctgcgcgg agacccggaa gatcccggcg agtttgaagg
     6121 cgttcgtcga gcgacacggg atgatcgcgg cggcctcccg cttcgatccg catctcttgc
     6181 agagtcgtct cgccgccctc gtcagggagc gcctcgaacg gacggtcgtt tatcagggag
     6241 gactcatcga gatcgacggc cgggggctcc tgctcgtcgg cgatagcggc tgcggaaaga
     6301 ccacctgtgc gctgtgtctc gcgaagcgcg gtgcatggtg gatcgccgat gatcgggtcg
     6361 aaatcgttcg cctgaggcat cgcctcctgg gtcgggcccc ccggtccatc cacagacttg
     6421 tcgcgctgaa aacgcaaggc gtggcggacg tcacgcagtt tgtcgacccg cagaccatca
     6481 aggcatcgtc ggacatcgct ggtgtcgtca cgcttgtttc cccgcgcgag ggcgcgcacc
     6541 gaaagacaag gggaggagaa cgagaaacca tcctggggat cgatctgccg cgggtgacga
     6601 tttccgtccc ggctagggga ggcgcatgcg ggtgggtagt cctttccagg ggcgctccgt
     6661 ccgcttttgc cttcctggtt cacgggccct gtcctctcgg gaacgcccgc tcggaaatgc
     6721 aagggggagg ttcgtcatga agcatcttcg cgtggtgatc atctccggcc tgtccggttc
     6781 cggtaaaagt acggccctga aggccctcga ggacatcggt ttctactgcg tcgacaacat
     6841 gcccgtggtc ctcctgccga agtttctgaa gatccagtcc gatccggcca aacagatcga
     6901 ccaggtcgcc atggtcatgg acctgaggga aaagagcttt ctcgaaaagt acccgcgcat
     6961 cttcaaccgc cttcgccagc agggataccg aatcgaagtc attttcatcg acgcgaacga
     7021 tgaaatcctc ctgcatcgct tcagcgaaac ccgccgcgtc cacccggtgt ccgtgaaagg
     7081 ctcggtgatg gatggcatcc gcctggagag ggagatgctc ttcccgttga aggaaatggc
     7141 ggaccgggtg atcgacacct cgtcgctcaa cgttcaccag ctcaaggacg ccgttcagcg
     7201 cgcctttctg gcgtcatcgg acaagaagcg cctcatcgtg aacgtggttt ctttcggata
     7261 ccggtatggc ctgcccgccg acgccgacgt tgtcttcgat gtccgctttc ttcccaaccc
     7321 ccactttgtc gatgagctga aacgccacga cggccatcat cccagcgtcc ggaattacgt
     7381 catggcgacg gatgaggcga aggggttcat ccagcggctg ttcgatctga tggacttcat
     7441 cgttcccctc tacgagcggg aggggaaggc ccggatcaac atcgccctgg gctgcacggg
     7501 gggacaccac cgctccgtcg tgatggcgaa ccagctgggc ggctactttt ccgagaagga
     7561 ctacctcgtc aacatcaccc accgcgacat cacgaagagc tagagcgacc ccgcctgcag
     7621 tttcttcaac acgttgtcct cgatccagtg ggggtcccac cattcccgag gatcgacgaa
     7681 ctgaccgccc accaggatgc tgaaatggag atgatcgccc ccggccagcc ccgtggttcc
     7741 ggagcggccg atgacgtctt cccgctggac ggcctgtccg acgcgggcgt cgatggcgct
     7801 caggtgcccg tacaggctga acagtcccag gccgtggtcg atcaggacgg cgtttccata
     7861 gattcccagg ggaccggtga agacgacgat gccgttgttg gcggcttcaa tggcggaatt
     7921 gttcaaagag gcgaggtctt cgcccaggtg caggctctgc ccgacgacct ggccctggta
     7981 ttcccacgtc cgccggtcgc cgaaaagggc catgggggcc gcgttcttca tccggaggaa
     8041 gggaccctcc cagagcgcgc ggggctgtgt cttccggcag aggtcccgga tggtctgctc
     8101 attctccagc cgcagggtgc cgttcacgta ccggaagact tcaatggcgg tcttgccccg
     8161 cagggccgga atggccgact ggaactccgg catcttctgc tggaggaaag cgtcgcccac
     8221 ggccattttg tccgagcgga acttccggga tttcatcgta cggggtatgg cgctggaggc
     8281 ggcatttcct gcggcgtccc gggcggtaat acgcatgtcg acgagacgct cttgggcgtc
     8341 catcgggatg gcgaagtagc agatgtaaga agcccggccc gcgatcagga cgggatgggc
     8401 ggggaagaag gcctcgtcga cctgaacgcc cgtctgcacc gccggcttcg agaggcggta
     8461 ggtgacgacc ccggccccgc cggggaagaa gatgttgacg ggattgttcg gaaagatttg
     8521 gggaggcgac agttcgatcg tcaccgggcg ggtgacgacg gtcgtgttgt tccagaggga
     8581 gcgatcggag gcggagaccg tcaggacggc tgacccttcg cggagcttca gggcgagcgg
     8641 atcgatcggg acgttcacgg tccgttggcg taggggcttg tcgtcaagcc gtactgatgc
     8701 aaccggcagg gtctggctgc cctgggtaat ctggacgctc gcggcgctga ggccacggtt
     8761 gtcccggagc tccagggtca ggacgccccg ctggccgagg ctgcgcaggt cctcggaaag
     8821 ccggatttcg ggcttctgcc actcgccggc ggtgctgaaa aaccagaata gaccgccggc
     8881 gactacgacg atcgcacaga tcagggagat ccagaaaaat gaagatttct tccgcatggc
     8941 gctaccctcc gggtacgggg atcggatgtc cggaaatcgg acgataaagg acgcgacttt
     9001 atagccgcat tcccgatgtc atgcaagcgg tttgttcgcg cgccatttcc ggttgacacc
     9061 atccggattt gccgatagta tccgagcctg tcaagacgct gcatttcgtc gcgaggggcc
     9121 tgccgccatg tggaagggga aaaacgttgt cctggggatc accgggggaa tcgccgcgta
     9181 caaggcggcg gagctgacac gggagatcgt tcgccgggag gcggccgtca gggtcgtcat
     9241 gacccggcat gcccaggagt tcatcgcccc cctgacgctt gagacgctct ccggcaaccg
     9301 tgtctgtacg gacctgttta cgccggaggc accgcacgag atcgatcata tcgccctggc
     9361 acgctgggcg gatgtcctga tcgtcgcacc ggcgacggcc aacctgattg ggaaggcggc
     9421 cggtgggatc gccgacgatc tcctgaccac caccctcctg gccacgcggg cgccggtgct
     9481 cctctgcccc gccatgaacg acgccatgtg ggagaatccg attgtcgggg agaacctgga
     9541 ccggctccgt caccgcggct gcgccgtcgt cgaaccggcc cgggggagcc tggcctgcgg
     9601 gacggagggc gcgggccggc tggccgatct cggcgatctt ctggacgccg cggaggccct
     9661 gttcaccccc caggattttt ccggggaaac ggttctcgtc acggcgggcc cgacccgcga
     9721 gccgctcgat ccggtcaggt tcatcaccaa ctattcctcc ggtaagatgg gctatgccat
     9781 cgcccacgcg gcccggatgc ggggcgcgac ggtgattctg gtcagcggtc cgaccgtcct
     9841 ggtcccgccg ggcggtgtgg aactcgtgca ggtgcagagc gcccgcgaga tgcgggatgc
     9901 cgttctgggc cgcctcgatg agaccacggt aatcgtcaag gccgccgccg tcgccgatta
     9961 ccgccccacc gtctgcgagg atcagaaaat caagaaacgg ccgggggacc tccggctctg
    10021 cctcgagcga aacccggaca tcatcgccga aatcggtcgg aagaaggacg ggcggattct
    10081 cgtcgggttc gccatggagt cagaaaatct gctcgaaaat gcacgcacga aaatgcgcgc
    10141 caaaaagatg gacctgatcg tcgccaacga tctgagggaa gcgggggctg gtttccaggg
    10201 cgacacgaac gtcatccgca tcctgagtcc ggacggccgg gtggaggcgc ttcccctgat
    10261 gggcaagcgc gaggcggccg atcggatcct ggatcggatc cgggaccttc gtggccgcag
    10321 tgggaggtga acgcgtgcgc gaggcctttc aggagcttgt tcatcatttg cacgcccggc
    10381 tgcgggagga ccaggcgcgc ggcggtcgcc tatcctacct cgtcgccgcc ggggactccg
    10441 gggtcgggcg ggaggccgcg ccgttcccgg agacggcttc acggaagacg ccgcctcggg
    10501 acaggacggt gcctgtccgc gccgccggcg agaccctgga ggcgatccgt caggccctgg
    10561 gcgactgcca acggtgtccg ctcggcgcaa aacgccgccg gctcgtcttc ggggaagggg
    10621 atccacacgc ggaactcgtc tttatcggag aggccccggg ggcggacgag gacctccagg
    10681 gacgaccctt cgtcggccgc gccgggcagc tcttgacgaa aatcatcgag gccatgggtt
    10741 tgaagcgcga cgacgtttac atctgcaaca tcctcaagtg tcgaccgccg ggaaaccgca
    10801 atcccgcgcc cgacgaagtc gccgcgtgcg aaccctttct gatccggcag ttgcaagcca
    10861 ttcgtccgcg cttcatctgt gccctgggat cggtcgccgc ccggacgctg ctcaagacgg
    10921 aagcgccgat caccgtcctg cgggggcgct ttcaaagcta tcagggaatc cggctcatgc
    10981 cgacgtacca cccggcctat ctcctccgga atcccgtggc gaaaaagaag gtctgggagg
    11041 acgtgcagat catcatgaag gcgttggacc ttccgattcc ggaacgaaaa aacgggttgc
    11101 ccggcagagg cgccccggca ggcgacgcgg aggggatatg atgggaccgg tcgaacaaag
    11161 gcacttgccc ctgatcgtcg tggtgatgct cgtgctgctg tcggcgacgg cggacgccgt
    11221 gccgggaagg tcacccactt tcaccgtgat ccgggtggac gccgccatca cgccgcctgt
    11281 cgcccagtac atcctgcaga gtctggaaga ggcgacccgt acgcgcagtg acggtctcgt
    11341 gattcggctc gacaccccgg gggggctcga cctcgccatg cgggatatcg tcaagggtat
    11401 tctgggcgcc gacatccccg tcatcgtgta tgtgtcgccc gcgggcgccc gggccgcttc
    11461 ggccggcatg atgatcacgg tggccgcgca cgtggccgcg atgacacccg gaacaaacat
    11521 cggcgccgcc caccccgtgg gcatcggcct gggcgggggc atggacaaga tcatgcaggc
    11581 caaggtggag aacgacgccg tggcttacat ccgtgggatc gccaaaggcc gggggcggaa
    11641 tccggactgg gtcgaacggg cggtccggaa aagcgcctcc gtttccgcgg aagaagccct
    11701 gaagctcggc gtcatcgatg aggtggcacc ggacctcgcg cacctgctgg cccggatcga
    11761 cgggaaggtg gtcaccctgg catcgggcaa aagggtgctg aagacccgag gggcggtcac
    11821 ggtcgaaaaa aagatgggcg ttcgccaagg aattctcacc gtgctcgccg atccgaacat
    11881 cgcctacatt ctgctgctga tcggactcgc gggcctctac ttcgaatttt cccatcccgg
    11941 cgccattctt cccggggtca tgggcgggat ctcgctgatc ctggcctttt tcgcccttca
    12001 gaccctgccg gtgaactacg cgggggtcct cttgatcctc ttcggcatca ccctcttcat
    12061 cgcggaggtc aaggtcatga gtcacgggat tctcaccgtc ggcgggatcg tttcactcgt
    12121 catggggtcg ctcatcctct tcgattcgcc ggaaccggcc ctccgggtgt ccttccaggt
    12181 catgattccc accctgatcg tcatctgcct gttttttgtg gccgtgatca gtctcgtcgt
    12241 aaaggcgcac cgcggccgga ggttcacggg tatggaaggg atgctcgggc tggaggggaa
    12301 ggcggtcaac gcggtcgggc cggacgcggg aaccgttctc gtcaagggcg agtactggcg
    12361 ggcgcggagc gatgaaccga tcgcgccagg cctaaagatc cgcgtgatcc gggtggacgg
    12421 cctccgcctg cttgtgggag aaattcatcc gggcgaaccg taaggcgcgc gagatgggcg
    12481 acggtcgccg cggccggcac ccggaaggcc cctgatcgca tccgctccgc tctaggacac
    12541 gggcgtgcgg aagagaccat tcgaaccgtc aaactacggg caaggaggga attatgtctg
    12601 ttacagtcat cgtcatcgtc gttttggtcg tgatgtttct ggcctcggcc attcggatcc
    12661 tcaatgaata cgaacggggc gtgatctttc gtctgggccg catcatcgac cagaaagggc
    12721 cgggactcat catactgatc cccgtcatcg acaagatggt caaggtggac atgcggacga
    12781 tcaccctgga tgtgccgccg caggacgtca tcacgcgaga caacgtgtcc atcaaggtca
    12841 acgccgtggt ctatttccgg gtcatggacg ccaattccgc cgtgatcgac gtcgagaact
    12901 tcctctatgc aacctcccag ctcgcccaga cgacgctccg gagcgtctgc gggcaggtgg
    12961 aactggacga gatcctctcc cagagggaga agatcaatct tcacctgcag gagatcctcg
    13021 atcgcagcac cgactcctgg gggatcaagg tctcgctggt cgaggtgaag cagatcgatc
    13081 tgccggagga gatgaagcgc gcgatcgcca agcaggcgga ggcggagcgg gaacggcggg
    13141 ccaaggtcat caacgcggag ggggagttcc aggccgccca gaagctgatc gaagcggcgg
    13201 cgctgatgga gacgcagccc atgtcgctgc agctccggta tctccagacg ctcaaccaga
    13261 tcgcctccga gaacaactcg acgacgctct tcccgatccc gatcgacctg ttcaagccgt
    13321 tcgtgaagct ggccgaaaaa tgagtcgggg cggaggcgaa gcgcgaagaa cgaccatgaa
    13381 aggagaacag agagcgtgca cggcaaagac aagcggcatc tgctgaccgg agggctcgtc
    13441 ctgatcgccc tcggcgtcct catcatcctc gacaagacgg ggatctacac cttctcacac
    13501 agctggccga tcctgctcat cgtcatttca ctgggcgtcc tgctccagcg cgtcaaggat
    13561 ctcggcggct ggatcatcgg cgcgtccggc gtggtttttc tcatcacgga gaactggggg
    13621 taccgcctct tcgagatcgc aaagtacctt ctgcccatca tgctgatcat catcggcgcc
    13681 gacctgatcc gcaggcgggc acgaaaatcc tgatgcggga cgaaggatcc gggccggggg
    13741 cggaccgcta cggcccgaca cggctcgcgt gggaaggaag atgaaccggg ttctgttgat
    13801 gagcgacttc gacgggacga tctgcagcgt tgatgtcgga gacggactcg gggctcgttt
    13861 cgcggccccc gagtggcggg atattgatcg ggcctacaac gagggccgga tcggttcccg
    13921 tctcgcctac ccgaggattg ccgccctgat gcggggcttg acccgggatt accttctcgc
    13981 cttcgcactc gagcgggcac gcctcgaccc cggtttcccc gcgttcgtat ccttctgccg
    14041 ggaacgcggg tatgatttca tgatcgtatc cgacggcctc gatgtgtaca ttcgggccat
    14101 cctcgaacgc gagggtttgg ccgacatcga attctacgcc aatgccgttt cgtttccgga
    14161 ggcggatcgg atcgccttcg ccttcccgca tgccaacgtc cgctgcggca agtgcgggac
    14221 gtgcaagacc gggcttctcg cccgacagcg gcgacgccat gacctaatcc tctatgtcgg
    14281 ggacggtcat tccgacgtat gtcccgcaca gcacgccgac ggcgtcttcg ccaagggcat
    14341 tctgcgcgaa aaatgcctgc tgaacgggac gccgttccgg tcctaccgaa acttcacgga
    14401 tatccagcgc atcctcgccg ccgctgaacc tggggcgctc gggagcatct ggaaggacgg
    14461 gcttgaggaa ggcccggaga aaggggaaag acatgtttca ggtggcctca tatgagacct
    14521 attcggacgg aaaggtgatt ttcaccgaag gaagccacgg cgactggatt tacgtcgtcg
    14581 aggaaggcgc cgtggagatc tccaagaaag acggagaaca gaaggtcgtc atcgaagtgc
    14641 tgaaacccgg agacctgttc ggcgagttgg cctacatcgc gaagattgca cgcaccgcca
    14701 ccgccaccgc ggtcggtaaa acggtcatcg gcatcctgga tcgggactat ttcgaccggg
    14761 agttcaacaa attgcccggc gacttccagg tcatgttcaa gacggtggcg ttgcgcctca
    14821 aggccaccac cgaaaaggtc accgcagcgt accgcggctg atttcccgcg aaacggaagc
    14881 gcgggacaga tgaaccgtcg acggaccggg tcccgcgtct ggcaggtttc ccgatctcag
    14941 gacaaaaagc gggcgaggaa gtcggcacag acgagccccc ggcgtgtggg tgaaaaacgg
    15001 tacccgtccg cccggacgag ccctgccgcg gcaagttgag cgatcttgtc gccccgctcc
    15061 gcgcaaagat cgaacccgta ccgcttccgg tacgcctcca tatcaattcc ctccctcgtt
    15121 ctgagcccca ggaaaagggc ctccagacgc aactgatcca gcgtgagtat ttcttctccg
    15181 gcgatcggcc gcatcccgtc ccgcagggcc ccgacccagg cgtcaaagtc gcgaggattc
    15241 caccagcgcc gggtgccgtc gaaggaatgg gcggagggac cgaggccgag gtagggggtg
    15301 tgatcccagt atttgcggtt atggcgggcg cggtgatcgt cgtcgcaggc gaaattcgac
    15361 acttcatagt gccggtagcc ggcggcttcc aggcgctctg aggtggtcag gaagtatcgg
    15421 gccgaaaggt cgtcatcggg aagggagagc ggagacagtg cgagcgtcgc cgcgagcggc
    15481 gtgtccgcct cgagggtgag ctggtagcac gacagatgct cgggttgaaa ggaaagggcg
    15541 gtctggagac tcgccagcca gccccggaag gccgttcccg ggtcgcctcc gggagcggga
    15601 aggccgtaga tcaagtcgag gccgagatcg tcaaagcccg ccgcgcggac agcgagaatc
    15661 gcgtcgcggc actgaccggc gctatgacgg cggccgagga aggcgaggac ggcgtcgtcg
    15721 aaggattggc agccgagggt gaggcgattg acgccgagcg cgcgcagcgt tcggagggcc
//
LOCUS       DAOOLJ010000037        15661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3976_length_15661_cov_18.392798, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000037 DAOOLJ010000000
VERSION     DAOOLJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15661
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_15661_cov_18.392798"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..79
                     /locus_tag="PLW16_07055"
     tRNA            5..79
                     /locus_tag="PLW16_07055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Val,seq:gac)
     gene            complement(58..204)
                     /locus_tag="PLW16_07060"
     CDS             complement(58..204)
                     /locus_tag="PLW16_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70700.1"
                     /translation="MNVLILKVFGGFDRPALKKGHHHLEAADALRKITGSAAVIKNGR
                     HAGI"
     gene            203..2122
                     /gene="thrS"
                     /locus_tag="PLW16_07065"
     CDS             203..2122
                     /gene="thrS"
                     /locus_tag="PLW16_07065"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPB70701.1"
                     /translation="MTKMLHIQLPGGATAEHPAGTPIREILAAWRPQALAAAVAARMD
                     GRAVDLDAVVEQDTGLEPIDLDSREGLSVLRHSTSHVMAQAVQDVFPGAKVSIGPSIE
                     DGFYYDFDYETPFTPEDLDKIEARMREIIAADHSFVREEMSRAAASEKFAAMGETYKV
                     ELLRDLPADVETVSLYTQGGYVDLCRGPHLPSTGRIAAFKLLNVAGAYWRGDERNRML
                     QRIYGTAFADADALAEHLKLVEEARKRDHRRIGRELDLFQMNDEAGPGLIIFHPKGML
                     LRTIIEDWERREHLKRGYDIVMGPQILKADLWKRSGHYDHYRENMYYTEVEGQPYGIK
                     PMNCLAHMLIYRSKIRSYRDLPIRYFELGTVHRHEKTGVLHGLMRARQFTQDDAHILC
                     RPDQLNEEIRAIAAFVADAMRIFGFDYEVELSTRPADSIGSDADWNLATTALEQALKD
                     TGLPYEVNVGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLTYIGADGEKH
                     RPVMLHRVILGAIERFMGVLIEHYAGAFPLWLSPVQAVLVTVTDAQIPYGETVYQTLL
                     DADIRVERDFRNEKLGFKIREAQVQKTPYMLVIGDREVASGQVSPRERSGKNLGAMTP
                     GAFAALVRERCANYE"
     gene            2158..2649
                     /gene="infC"
                     /locus_tag="PLW16_07070"
     CDS             2158..2649
                     /gene="infC"
                     /locus_tag="PLW16_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPB70702.1"
                     /translation="MNREIRAPQVRVIDAEGKQLGVIPLADALAEAGRVNLDLVEVAP
                     AANPPVCKIMDYGKFRYQQSKKLALAKKSQSTIQVKEMRIRPKTEAHDLEVKIKHIRK
                     FLEQSDKVKITMMFRGREIAYTENGRRIMESVKESLAEISQVDQYPKLEGRNMVMILS
                     PKK"
     gene            2678..2875
                     /gene="rpmI"
                     /locus_tag="PLW16_07075"
     CDS             2678..2875
                     /gene="rpmI"
                     /locus_tag="PLW16_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPB70703.1"
                     /translation="MPKFKTHRGAAKRFSLTGTGKIKRGKAYHRHILTSKTRKRKRNL
                     RKGVIVDAANVRAIRKLIPYL"
     gene            2919..3278
                     /gene="rplT"
                     /locus_tag="PLW16_07080"
     CDS             2919..3278
                     /gene="rplT"
                     /locus_tag="PLW16_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB70704.1"
                     /translation="MSRVKRSITAKKKRRKILKLAKGFFGARSRLLRTATEAVDRAMR
                     YAYRDRRARKREFRQLWIARINAGARANGISYSRLIEGMKKQGVEIDRKVLSDLAIHD
                     PQAFTKVVAVARGEQTS"
     gene            3275..4291
                     /gene="pheS"
                     /locus_tag="PLW16_07085"
     CDS             3275..4291
                     /gene="pheS"
                     /locus_tag="PLW16_07085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB70705.1"
                     /translation="MKEALEELYRQAEAELKAAAGEADLLAVKTKYLGRKGLLTAALR
                     QVSQAPAEERPALGRRANEIKEMLAARIEAALQDLAAGRKEAALLAERIDVTLPGRGT
                     SPGRLHPITQTTREICRIFAGFGFSVAEGPEVERDYYNFEALNMPPDHPARDMQDTFY
                     IEEGVVLRTHTSPVQVRTMEKQRPPVRIISPGRVYRPDSDVSHTPMFHQVEGLLVDRG
                     ITFGDLKGLLAAFLKQIFGDETVLRFRPSFFPFTEPSAEVDIRCVMCGGKGCRVCGQS
                     GWLEILGSGMVDPAVFENVGYDPEEVTGFAFGLGVERIAMLKYGINDIRLFFENDVRF
                     LDQF"
     gene            4395..6812
                     /gene="pheT"
                     /locus_tag="PLW16_07090"
     CDS             4395..6812
                     /gene="pheT"
                     /locus_tag="PLW16_07090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB70706.1"
                     /translation="MLVSLRWLKDYVEMDLGPEALAERLTMAGLEVDAVTQAGPAFTN
                     VVVAKILAVKPHPGADKLFLCDVTDGETTWPIVCGAPNTRAGILSPLARVGATIPGGY
                     TIKSSVIRGERSEGMLCSEEELGIGPDTSGILVLPEDLPLGLDLADALDLKDTVLDIS
                     ITPNRADCLSIVGIAREVAALTGRPLRYPEIRIAEAPRAAADVTSVTILDPDLCPRYT
                     ARVIEGVTIGPSPRWMRQRLEAVGLRSINNIVDITNFVMMELGQPLHAFDFRFLEEGR
                     IVVRRSTEGEPFISLDEKERILKADTLMICDGRKPVAIAGIMGGLNSEVKEDTAMILL
                     ESAYFAPSSIRRSSRTLGMGTDAAFRFERGIDPEGVVRAADRAAQLMAELGGGQVLQG
                     IIDCYPQTVPVAKDIPLRLERINDILGTEVPAEAAAGILKALGMEVRGEADGTWRVTP
                     PTYRVDVTREIDLIEEVARLWGYDHVPTTLPSAAAAPVRKAPKEHAADRIREVLTGAG
                     YTELVNYSFISQTAPDWLGLAADDPRRRLVRISNPLTEDQSVMQTTLIWGLIQTMARN
                     VRAGAGDLKLFEIGRLFFAGAAGELPVERDSIACLLTGLRYDDQWHHPDVRVDFYDLK
                     GVLEGLFRALKIDGIRFDSANPEPFLHPGRACRIFVGGQAAGILGEVHPDLLERIDVK
                     GRTVVCELDLDALTAATRAVSAFRGVSRFPAVARDVAFLVPRELESEALLGHIRETGE
                     ELLEKVQIFDVYDGERIEPGFKSLGLRFSYRKADKTLTDEEVQQVHTALVARVVERTG
                     ARVRGES"
     gene            6850..7122
                     /locus_tag="PLW16_07095"
     CDS             6850..7122
                     /locus_tag="PLW16_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HPB70707.1"
                     /translation="MTKIDIIQEVYEKLGFSKKDSAQIVESVFDIMKDTLAKGEKIKI
                     SGFGNFVVKEKKSRRGRNPQTGKEIEISARRVLTFKSSQVLRKALN"
     gene            7225..7563
                     /locus_tag="PLW16_07100"
     CDS             7225..7563
                     /locus_tag="PLW16_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB70708.1"
                     /translation="MDPAIPDKTYFRIGEVARLLDVETHVLRYWESEFKTVRPVRTRT
                     DQRLYRRQDVEELLRIRNLLYEERFTLDGAKRQLARLRREASPAGGGDEHERLRRIKD
                     GLLELKRIMQ"
     gene            complement(7620..7901)
                     /locus_tag="PLW16_07105"
     CDS             complement(7620..7901)
                     /locus_tag="PLW16_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HPB70709.1"
                     /translation="MLCQTIKTGTECAFMTKKGCGFNGGSCHVIVEKCEGCNKVMEVA
                     SGKYCRVYPDPAAKWGIRSCPTASHLKLDVKESNQKVNPLKASKRANKK"
     gene            8178..8930
                     /locus_tag="PLW16_07110"
     CDS             8178..8930
                     /locus_tag="PLW16_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HPB70710.1"
                     /translation="MDGWLLREIWFRSLTVQASLNLWRMQNLGFCYALLPWIRRAGGD
                     REEMTRRLLRHLQPFNTHPYLMAPVLGAVLHAESRPDAPADGAPATALKNALTGPYAA
                     IGDAFFWGAWRPFCGLISVTLAIMGSMAAPLVLLMLYNPPHLWVRTRGYVEGIRKGRG
                     GADFIAALDLPRRTRQIRWGSLVFLATAAALLPEGVEGQIPLVTGFTMKLALLFVMLA
                     SYWGIRKGLSPFLLLYGAFAVLLAAFTLLAAV"
     gene            8950..9225
                     /locus_tag="PLW16_07115"
     CDS             8950..9225
                     /locus_tag="PLW16_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPB70711.1"
                     /translation="MKEVIRTVRIVNRLGLHARAAAKLVHMANGFQARIFLERNGEAV
                     DAKSLLGILTLAGTRGSYITIRAVGADAGVAVEAIAALVARKFDEEE"
     gene            9258..11042
                     /gene="ptsP"
                     /locus_tag="PLW16_07120"
     CDS             9258..11042
                     /gene="ptsP"
                     /locus_tag="PLW16_07120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPB70712.1"
                     /translation="MKKTFVLKGVGVSPGVVTGKAYLFDRMDSQISFYRLENPKLIAR
                     EVTRYRRAVRESLRQMQDLQRRMGELEGMEALYIIDVHILILKDRSFLNRTVRNIKEA
                     GVNAEWALRMTIDTYREIFAKVEDDYLRDRISDIQYVGQRILQNLTGGQQPSFTRLGG
                     GVIIVAQDLSPADTAQMKIDKIFGFATDIGGKTSHTAIVARSMQIPAVVALEKITRIC
                     RTNDEIIIDGSSGLVIVNPDPELLRRYEEKKRHYLQLKADTQKYAELPAVTTDGYRVR
                     VSGNIEFVEEIPAALANGADSIGLYRTEFIYINREQLPTEEEHLASYRQVVSVPGLDG
                     ATIRTFDLGGDKFFSDPRLAKEMNPQMGLRAIRFCLKEVDLFKVQLRAILRAGAFGKV
                     RILFPMISGVEEIREAKRILAEVRRDLVAQRVDIGPDPEIGVMIEVPSAVIIADQLAR
                     EVDFFSIGTNDLIQYALAIDRINERVTYLYEPLHPAVLRLIRQVVEVGHQAGIRVAMC
                     GEMAADPLYVMILLGLQIDELSMNPQAIPRVKKIVRGAMLTESRELLERVMTFTSVGE
                     IRGYVEAFMKARFPDEFAVNGNMDLHAK"
     gene            11146..>12548
                     /locus_tag="PLW16_07125"
     CDS             11146..>12548
                     /locus_tag="PLW16_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB70713.1"
                     /translation="MSEKEHYPGSLYPRENILLGWLVRKALSRVAFADEQADAIRGLA
                     REGVVVHAVKTESQLNSLILRELSLRKNLPLPAWSYGANMLLWQPYAFALKGLLNRVN
                     PYRTAYLKRLVTDRRSAIIHLGGAEFLESPEAADALNQVLEAQRGLDVPVYIVPELVT
                     YGRRRETERESVLNILFGQTENTGPWLRVITFLRYANKAQVIAAEPVNVAEFLAEHEG
                     LPAAEQCRRLRQELIERIDAEKTTMVGPVLKSRAEINNLVLKDEDLLHFMDEHARRTE
                     KGFAAVRKEARRYLKEISSDYREMFVEIWDKFLTWLWNDIYDGVVVDREGMARLRDIS
                     RRMPFVIIPCHRSHIDYLLLSYVFYKNNIQLPFIAAGTNLMFWPLGAIFRKSGAFFLR
                     RSFKGNPLYAEVFAKYVKTLLREGLPIEFFIEGGRSRTGKMVMPKYGLLSIIMQSFIE
                     GCCQDLAAVPVYIGYDRV"
     assembly_gap    12549..12648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12649..13347
                     /locus_tag="PLW16_07130"
     CDS             <12649..13347
                     /locus_tag="PLW16_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70714.1"
                     /translation="LEDEIPVEDIEEVVYSLEDDKRLNLEYYKNNILHYFLPIAFVAV
                     SVLSSREDLIAFSRILEDYGFFKRLFRHEFIFDDDRTDLDEVNEVLTYLHDRGMIAGE
                     ERGEEALIEIKGRGRTNLRAYAGLIHNYLESYWVVVRGCAHLKAGEVPEREFLRRLQT
                     LGLKMFKKGEITRAESLSQANYQNALRFLRERGVLVAEERRDKGEKRETRYLSLAEDR
                     APLEALRRRLFQFM"
     gene            complement(13491..14783)
                     /gene="eno"
                     /locus_tag="PLW16_07135"
     CDS             complement(13491..14783)
                     /gene="eno"
                     /locus_tag="PLW16_07135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB70715.1"
                     /translation="MTDIIQVHAREILDSRGNPTIEAEVTLLSGIVGRASVPSGASTG
                     EHEMLELRDGVKKRYGGKGVRKAVRNVVEKIGPEIIGLDCLRQREIDRTMIALDGTPN
                     KGNLGANAILGVSLACARAAAEALDLPLYRYIGGVGAKDIPVPMCNILNGGQHADNNV
                     DIQEFMVMPVGAKSFAEGLRMAAETFHRLKAVLKTRGYNTAVGDEGGFAPNLKSNEEA
                     LEVIVEAIVQAGYRPGKDVAIALDAAASSFYAGGKYRLAAEKKPVKTAEEMIAFYAAL
                     VKKYPIVSIEDGLAEDDWAGWKLLTDALGGRVQIMGDDLFVTNAQRLKRGIDAGIANS
                     ILIKLNQIGTLTETLETIQMAKEAGYTTVVSHRSGETEDSFMADVAVGMNCGQIKSGS
                     LSRSERLAKYNQLLRIEEDLGDAAIYRGPAALYSVHKK"
     gene            15159..>15661
                     /locus_tag="PLW16_07140"
     CDS             15159..>15661
                     /locus_tag="PLW16_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB70716.1"
                     /translation="MEEKRTRLHLHLKKWLEKAVGDYGMIAAGDRVLVGVSGGVDSFV
                     LLGLLNSPMIFTPPFSILAAHIDLGFAPKGEGAARVAARLAETGIPLVSERTDIGPLA
                     HSEVNRKNPCFLCSRLRRRRLFEIAAAEGCTKVALAHHRDDIIETLLINLFFGREIST
                     MTPNQPL"
BASE COUNT         2831 a         4799 c         5208 g         2723 t
ORIGIN      
        1 tcgtaggcac gtagctcagg gggagagcgc catcctgacg cggtggatgt ccagggttca
       61 aatccctgcg tgcctaccat ttttgatgac ggcggcggaa ccggtgattt tgcggagggc
      121 atcagcagcc tcgaggtgat gatgcccttt ctttaatgcg gggcggtcga atccgccaaa
      181 taccttcaat atcaatacgt tcatgacaaa gatgctccac atacagttgc ccggcggggc
      241 gacggcggaa cacccggcag ggacgccgat acgggagatc ctggcggcct ggcggccgca
      301 ggcgctcgca gcggccgtcg cggcccggat ggacgggcgt gcggtcgatc tcgacgccgt
      361 tgtcgaacaa gacaccgggc ttgagccgat cgacctcgat tcgagggagg gtctgtcggt
      421 gctgcgtcac agcacgtccc acgtgatggc ccaggccgtc caggacgtgt ttcccggtgc
      481 gaaggtgtcg atcggaccgt ccatcgagga cgggttctac tacgacttcg actacgagac
      541 accgttcacg ccggaggacc tggacaagat cgaggcgcgg atgcgcgaga tcatcgccgc
      601 cgaccattcc ttcgtgcgcg aggagatgtc gcgtgcggcc gcgagcgaaa agttcgccgc
      661 gatgggcgag acctacaagg tcgaactcct gcgcgacctg cccgcggatg tcgagaccgt
      721 cagtctctac acgcaggggg gctacgtcga tctgtgccgc gggccgcacc tgccgtccac
      781 ggggcggatc gccgccttca agcttctgaa cgtcgccggg gcctactggc ggggcgacga
      841 gcgcaaccgg atgctccagc ggatctacgg gacggccttc gccgatgccg acgcgctcgc
      901 ggagcacctg aaactcgtcg aggaggcgcg caagcgcgac caccgccgga tcggccggga
      961 actcgacctc ttccagatga acgacgaggc ggggccgggg ctcatcatct tccacccgaa
     1021 ggggatgctg ctgcgcacga tcatcgagga ctgggagcgc cgggagcacc tcaagcgcgg
     1081 ctacgacatc gtcatggggc cgcagatcct gaaggcggac ctgtggaagc gctccggcca
     1141 ctacgaccac taccgcgaga acatgtacta caccgaggtg gaaggccagc cctacgggat
     1201 caagcccatg aactgcctgg cccacatgct catctaccgc tcgaagatcc ggtcctaccg
     1261 cgacctgccg atccggtact tcgaactggg gacggtgcac cgccacgaga agacgggcgt
     1321 gctgcacggg ctgatgcggg cgcgccagtt tacgcaggac gacgcgcaca tcctgtgccg
     1381 gcccgaccag ttgaacgaag agatccgggc gatcgcggcc ttcgtggccg acgcgatgcg
     1441 aattttcggg ttcgactacg aggtcgagct ctcgacccgg ccggcggatt cgatcggctc
     1501 cgacgccgac tggaacctgg cgacgaccgc gctcgagcag gccttgaagg acacgggcct
     1561 tccgtacgag gtcaacgtcg gcgacggggc cttctacggg cccaagatcg acttcaagct
     1621 gaaggacgcg ctcaagcgca agtggcaatg tgccacgatc cagtgcgact tcaccctgcc
     1681 ggagcgcttc gatttgacct atatcggggc cgacggggag aagcaccggc ccgtcatgct
     1741 gcaccgggtg atcctggggg cgatcgagcg cttcatggga gtgctcatcg agcattatgc
     1801 aggcgccttt ccgctgtggc tctctcccgt ccaggcggtc ctcgtcacgg tgacggacgc
     1861 gcagatcccc tacggcgaga cggtctacca aacgctcctg gacgcggaca tccgcgtgga
     1921 gcgcgatttc cggaacgaga aactgggctt caagatccgc gaggctcagg tgcagaagac
     1981 gccgtacatg ctcgtcatcg gcgaccgcga ggtggcttcc gggcaggtgt cgccccggga
     2041 gcggtcgggc aagaacctgg gggccatgac acccggggcc tttgccgcgt tggtgcgcga
     2101 gcgctgcgcg aactacgaat agacttcagg aggtgaccca tagctaagga tctgaatatc
     2161 aatcgggaaa tccgggcccc gcaggtccgg gtgattgatg cggaaggaaa acaactgggc
     2221 gtgattcccc tggccgatgc gctggcggag gccggccggg tgaacctgga cctggtcgag
     2281 gtcgcgcccg cggcaaatcc tcctgtctgc aagatcatgg actacgggaa gttccggtat
     2341 cagcagagca agaagctcgc gctggccaaa aagagccaga gtaccatcca ggtcaaggag
     2401 atgcggatcc ggcccaagac ggaggcgcac gatctggagg tgaagatcaa gcacatccgg
     2461 aagtttctgg agcagagcga caaggtcaag atcacgatga tgttccgggg gcgggagatc
     2521 gcctataccg agaacggccg gcggattatg gagagcgtca aggaatcgct cgcggagatc
     2581 agccaggtgg accagtaccc gaagctcgag gggcgcaaca tggtgatgat cctgtcgccg
     2641 aagaagtgaa gtcatttttg aaaaggagtc gagagtcatg ccgaaattca agacgcaccg
     2701 gggggcggcg aagcgttttt ccctgacggg gacgggtaaa atcaagcgcg gcaaggccta
     2761 ccaccgccac atcctgacca gcaagacccg gaagcgcaaa cggaacctgc gcaaaggggt
     2821 gatcgtggac gccgccaacg tgcgtgcgat ccggaagctc atcccttatc tctaggcgaa
     2881 aggccaaagg ttaaaggtta aaagctaagg agcacatcat gtcacgggta aaacgtagca
     2941 tcacggcgaa gaagaagagg cgcaagatcc tgaagctggc aaagggcttt ttcggggccc
     3001 gcagccgcct cctccggacg gccacggaag cggtcgaccg ggccatgcgg tatgcctacc
     3061 gcgaccggcg ggcacggaag cgggagttcc gccagttatg gatcgcccgg atcaatgccg
     3121 gcgcgcgcgc gaacggcatc agctacagcc gactcatcga gggtatgaag aagcagggcg
     3181 tcgagatcga ccgcaaggtg ctgtccgatc ttgccatcca cgatccccag gccttcacga
     3241 aggtcgtggc ggtcgcccgg ggtgagcaga cgtcatgaag gaagcgctgg aggagctcta
     3301 ccggcaggcc gaagcggagc tgaaagccgc ggcgggtgaa gcggacctcc tcgccgtcaa
     3361 gacgaagtac ctcggccgga aaggcctcct gacggcggcg ctccgccagg tgagtcaggc
     3421 gccggccgaa gagcggcccg cgctgggccg gcgggccaac gagatcaagg agatgctggc
     3481 cgcgcggatc gaggcggccc tgcaagacct cgcggccgga cgcaaggaag ccgctctcct
     3541 cgcggagcgg atcgatgtca ccctcccggg gcggggcacg agccccggcc gtctccaccc
     3601 catcacccag accacccgcg agatctgccg gatcttcgcc ggcttcggtt tttccgtggc
     3661 cgaggggccg gaggtcgagc gcgactacta caacttcgag gccctgaaca tgccgccgga
     3721 ccacccggcg cgggacatgc aggacacctt ctatatcgag gagggcgtcg tgctccggac
     3781 gcacacgtcg ccggtgcagg tccggacgat ggaaaagcag cgtccgcccg tgaggatcat
     3841 ttcccccggc cgcgtgtacc ggcccgattc tgacgtttcc cacaccccga tgttccatca
     3901 ggtcgaaggc ctcctcgtgg accggggcat caccttcggc gatctgaagg ggctcctggc
     3961 ggctttcctc aagcagatct tcggcgacga aaccgtgctc cggtttcgac cgagcttctt
     4021 ccccttcacc gagcccagcg ccgaagtgga catccgctgc gtcatgtgcg gcggcaaagg
     4081 ctgccgcgtc tgcgggcaga gcgggtggct cgagatcctc ggttccggga tggtggatcc
     4141 ggcggttttc gagaacgtgg gctacgaccc ggaggaggtg acggggttcg ccttcggcct
     4201 cggcgtggag cggatcgcca tgctcaagta cggcatcaac gacatccgcc tcttctttga
     4261 gaacgacgtc cgtttcctcg atcagttctg aaaaacgggg caacgtcccg atttatgaat
     4321 gagaacagag cgacgtcctg gttgatcgac tgaaagaaaa acagggtgac gtccctgttt
     4381 attgacggtg ggatatgctg gtcagtttaa gatggcttaa ggattatgtg gagatggacc
     4441 tggggccgga ggcgttggcc gagcggctga cgatggcggg cctcgaggtc gatgcggtga
     4501 cgcaggcggg gccggcgttc acgaacgtcg tcgtcgcgaa gatcctcgcg gtcaaaccgc
     4561 acccgggggc ggacaagctc tttctgtgcg acgtcaccga cggcgagacc acctggccga
     4621 tcgtctgcgg cgccccgaac acccgcgccg gcatcctgtc tccgctggcg cgcgtggggg
     4681 cgacgatccc cggcggctac acgatcaaga gctccgtcat ccggggcgag cgctccgagg
     4741 ggatgctctg ctcggaggag gaactcggca tcggaccgga cacctcgggc atcctggtcc
     4801 tgccggagga cctgcccctg ggcctggacc tggccgacgc cctggacctg aaggacacgg
     4861 tcctcgacat ctccatcacc cccaaccgtg ccgactgcct gtcgatcgtc gggatcgcgc
     4921 gggaggtggc cgcgctcacc ggccggccgc tccgctatcc ggagatccgc atcgcggagg
     4981 cgcccagggc cgcggccgac gtcacctcgg tcacgatcct cgaccccgac ctatgcccgc
     5041 gctacacggc gcgcgtcatc gagggcgtga cgatcgggcc gtcgccgcgt tggatgcgcc
     5101 agcgcctcga ggccgtgggg ttgaggtcta tcaacaacat cgtcgacatc acgaacttcg
     5161 tgatgatgga gctgggccag cccctgcatg ccttcgactt ccggtttctc gaggaggggc
     5221 gcatcgtcgt gcgccgttcg accgagggag agccgttcat ctcgctcgac gagaaggagc
     5281 gcatcctcaa ggccgacacc ctgatgatct gcgacggccg gaaacccgtc gccatcgcgg
     5341 gaatcatggg gggcctcaat tccgaggtca aggaggacac ggcgatgatc ctcctggaaa
     5401 gcgcgtattt cgcgccgtcg tccatccggc gttcctcgcg gacgctgggg atgggaacgg
     5461 acgccgcgtt ccgcttcgag cgcgggatcg accccgaggg ggtggtccgg gcggccgacc
     5521 gggcggcgca gctcatggcc gaactggggg gtgggcaggt gctccagggg atcatcgact
     5581 gctacccgca gacggtgccc gtggcgaagg acatcccgct gcgccttgag cgcatcaacg
     5641 acatcctggg gacggaggtc ccggccgaag cggcggccgg catcctgaag gcgcttggga
     5701 tggaggtgcg cggggaggcc gacgggacct ggcgggtgac cccgcccacg taccgcgtcg
     5761 atgtcacgcg cgaaatcgac ctcatcgaag aggtcgcccg cctctggggc tacgaccacg
     5821 tgccgaccac gctgccgtcc gcggccgccg cgcccgtccg caaggccccg aaggagcacg
     5881 cggcggaccg catccgcgag gtgctgacgg gcgcgggtta cacggaactc gtcaactaca
     5941 gcttcatctc ccagaccgcc cccgactggc tggggctggc cgcggacgat ccccggcggc
     6001 ggctcgtccg gatctccaac ccgctcacgg aggatcagtc cgtcatgcag acgacgctga
     6061 tctgggggct gatccagacg atggcgcgca acgtccgcgc cggcgccggc gatctgaagc
     6121 tcttcgagat cgggcgcctc ttcttcgccg gggcggcggg cgagctgccc gtcgaacgcg
     6181 acagcatcgc ctgcctcctg acggggctcc ggtacgacga ccagtggcac cacccggacg
     6241 tccgggtgga tttctacgac ctcaagggcg tgctcgaggg gctgttccgg gcgctcaaga
     6301 tcgacggcat ccgcttcgac tcggcgaacc ccgagccgtt cctccatccc gggcgggcct
     6361 gccggatttt tgtcggcggg caggcggccg gaatcctggg cgaggtgcac ccggacctgc
     6421 tggagcgcat cgacgtcaag gggcgcacgg tggtctgcga actggacctg gacgcgctga
     6481 ccgccgcgac gcgcgccgtc tcggccttcc ggggcgtttc ccgctttccg gcggtggcgc
     6541 gggacgtcgc cttcctggtg ccccgggagc tggaaagtga agcgctgttg ggccatattc
     6601 gcgaaacagg tgaagaatta cttgaaaaag ttcaaatttt tgatgtatat gacggcgaac
     6661 ggatcgaacc gggcttcaag agcctgggcc tgcggttctc ttaccggaag gcggacaaaa
     6721 ccctgacgga cgaagaggtg cagcaggtcc acacggcgct ggtcgcccgg gtggttgagc
     6781 ggaccggcgc cagggtgagg ggtgaatcct agacagggtc gcattcagac agcttcagga
     6841 gggtgagaga tgacgaagat cgatatcatt caggaagtat acgagaaatt gggcttctcc
     6901 aagaaggatt cggcgcagat cgtggagtcg gttttcgaca ttatgaagga caccctggcc
     6961 aagggggaga agatcaagat ctccgggttc ggcaactttg tcgtcaagga aaagaagtcc
     7021 cgccgcggtc gaaaccccca gaccgggaag gagatcgaaa tctccgcgcg ccgggtcctg
     7081 accttcaaat ccagccaggt gctgcgcaag gccctcaatt agaaaagacc cgcggggtcc
     7141 gtccggcgcc cctcgttttc ccgggatgag cggctccgcc ggttccgaac ccgcgttcga
     7201 cgtgtgccgg gggagggact cacggtggat ccggctatac ccgataaaac gtatttccgc
     7261 atcggcgagg tcgcccggtt gctggacgtc gagacccacg tcctgcggta ctgggagtcg
     7321 gagttcaaga ccgtccggcc cgttcggacc cggaccgatc agcggctcta ccgccggcag
     7381 gacgtcgagg aactgctccg catccggaac ctcctttacg aggagcgttt caccctcgac
     7441 ggggcgaaga ggcagctcgc ccgtctgaga cgcgaggcgt cgccggccgg agggggggac
     7501 gagcacgaac gcctccggcg gatcaaggac gggctccttg aactgaagcg gatcatgcag
     7561 tgaaggccga tgaagggaaa gacttcatcg gccttttcaa tgcaaacggg aaggggcggt
     7621 tatttcttgt ttgcccgctt ggaggccttg aggggattga ccttctggtt cgattccttg
     7681 acatccagtt tcaggtgcga ggccgtgggg cagctccgga ttccccattt ggcggccgga
     7741 tcgggataga cgcggcagta cttgccggat gcaacctcca tcaccttgtt gcagccctcg
     7801 cacttctcga cgatgacgtg acagcttccg ccgttgaagc cgcagccctt cttggtcatg
     7861 aaggcgcatt ccgtaccggt cttgatggtt tgacaaagca tgggtcgata cctctttctc
     7921 aacgtgtcgc aaatgttgct ttcgtccggc agggcggttc cctgcttggc gggcgcgcct
     7981 agctaccaga aaaaaaaagg cgtgtcaata aaaaagatat tcggaaaacg cgagggcgta
     8041 aatcgcgggg aatgaattga cttcacaggg caatcgtggt aggcaacgac cgttttttac
     8101 gtgtgtgcgc gggcccggaa tcgatgttac gttgccatga aaagggaccg tcgcgttcga
     8161 gggttttggg gtttggtatg gatggctggc tcctcaggga aatatggttc cgctccttga
     8221 ccgtgcaggc gtccctgaat ttatggcgca tgcagaatct cggcttctgc tacgccctgc
     8281 tgccttggat ccgccgggcg ggcggcgacc gcgaggagat gacgcggcgc ctgctgaggc
     8341 acctgcagcc gttcaatacg cacccctatc tgatggcgcc tgttctgggg gcggtcctgc
     8401 acgcggaatc gcggccggac gcgccggccg acggtgcgcc ggccacggcg ctgaagaacg
     8461 ccctgacggg gccttacgcc gccatcggcg acgccttctt ctggggggca tggcggccct
     8521 tctgtgggct gatctccgtg acccttgcga tcatggggtc catggccgcc ccgctggtgc
     8581 tgctcatgct ctacaacccg ccccacctgt gggtgcggac ccgggggtat gtggagggaa
     8641 ttcgaaaggg gcgcgggggg gccgacttta tcgcggcgct ggatctgcca cgccggaccc
     8701 gtcagatccg ctgggggtcc ctcgtgtttc tggccacggc cgcggccctg cttccggagg
     8761 gggtcgaggg ccagattccc ctggtgacgg gctttacgat gaaactcgcg ctcctgttcg
     8821 tcatgctagc gagctactgg ggaatccgga aggggctgtc ccccttcctg ctcctgtacg
     8881 gcgccttcgc ggttctgctc gcggccttta ccctgctggc agctgtttga gagggcgtga
     8941 cggaatccga tgaaggaagt catccggacg gtcaggatcg tgaacaggct cggactccat
     9001 gcacgggcgg cggcaaagct ggtccatatg gcgaacggct ttcaggccag gatcttcctc
     9061 gagcggaacg gtgaggcggt ggacgccaaa agcctgctcg ggattctgac cctggccggc
     9121 acccggggaa gttacatcac gatccgagcg gtcggggcgg acgccggggt ggcggtcgag
     9181 gcgattgcgg cgcttgtagc gcgaaaattc gacgaagagg agtagacatt cagaacggta
     9241 tgtcccctga tgatgagttg aagaaaacgt ttgtgctgaa gggcgtcggc gtgtctccgg
     9301 gggtggtcac cggcaaggcc tacctcttcg acaggatgga ctcgcagatc tccttctatc
     9361 ggttggagaa tccgaagctg atcgcgcgcg aggtgacccg ctaccgtcgt gccgtccggg
     9421 aatcgctccg gcaaatgcag gacctgcagc gtcgcatggg ggaactggag ggcatggagg
     9481 ccctgtacat catcgatgtg catatcctga ttctcaagga tcggagcttc ctgaaccgca
     9541 cggtccggaa catcaaggag gcaggcgtga acgccgagtg ggccctccgg atgaccatcg
     9601 acacctaccg ggagattttc gcgaaggtcg aagacgacta cctgcgcgac cggatcagcg
     9661 acatccagta cgtcgggcag cggatcctgc agaacctgac ggggggccag cagccgtcct
     9721 ttacccggtt gggcgggggt gtcatcatcg tggcccagga cctgtctccc gccgataccg
     9781 cccagatgaa aatcgacaag attttcgggt tcgccacgga catcggcggc aagacgtcgc
     9841 acaccgccat cgtggcgcga tcgatgcaga tcccggcggt cgtggccctg gagaagatca
     9901 cgcggatctg ccggacgaat gacgagatca tcatcgacgg gtcgtcgggg ctggtgatcg
     9961 tgaatcccga tccggagctg ttgcggcgct acgaagagaa gaagcggcac tacctccagc
    10021 tcaaggccga tacgcagaaa tatgccgagc tgcccgcggt gacgacggac ggctatcgtg
    10081 tccgggtcag cgggaacatc gagtttgtcg aggagattcc ggcggcgttg gccaacgggg
    10141 cggacagcat cgggctgtac cggacggagt tcatctacat caaccgcgaa cagctcccca
    10201 ccgaggagga gcacctggcc agttaccgcc aagtggtatc ggtaccgggg ctggacgggg
    10261 cgacgatccg caccttcgat ctcggcggcg acaagttctt ctccgacccg aggcttgcca
    10321 aggagatgaa cccgcagatg ggcttgcggg ccatccggtt ctgcctgaag gaagtggacc
    10381 tcttcaaggt gcagctccgg gccatcctgc gcgcgggcgc cttcggcaag gtgcgcatcc
    10441 tcttccccat gatctccggt gtcgaggaga tccgggaggc gaaacggatc ctcgcggagg
    10501 tccgccggga tctcgttgcc cagcgggtgg atatcggccc ggacccggag atcggcgtca
    10561 tgatcgaggt gccgtcagcg gtgatcatcg ccgatcagct tgcccgggag gtggatttct
    10621 tcagcatcgg gacgaacgat ctgatccagt acgccctggc gatcgaccgg atcaacgagc
    10681 gggtgaccta cctctacgaa ccgcttcacc cggcggtcct cagactgatc cggcaagtgg
    10741 tggaggtcgg gcatcaggcc ggcatccgcg tggccatgtg cggcgagatg gccgccgatc
    10801 cgctgtacgt tatgatcctg ctcggccttc aaatcgacga gctcagcatg aacccccaag
    10861 cgatcccgcg ggtgaagaag atcgtccgag gggcgatgct gaccgaatcc cgagaactcc
    10921 tcgagcgggt catgaccttc acctcggtcg gggagatccg gggctatgtc gaagccttca
    10981 tgaaggcgcg ttttccggac gagtttgccg tcaacgggaa catggatctg catgcaaaat
    11041 agcgtttagg cgggcggacg ttcccgcccg tcggggaatg gccccggcgc gcctgatttc
    11101 gggggcgccg gagaagggcg tttcctcgag gggggagaaa tcgagatgtc ggagaaggag
    11161 cattacccgg ggagtcttta cccccgcgaa aacatcctgc tgggctggct tgtgcgaaag
    11221 gccctctcgc gggtcgcctt tgcggacgag caggccgacg cgatccgcgg gctcgcgcgc
    11281 gagggggtcg ttgtccacgc cgtcaagacc gagagccagc tcaacagcct tatcctgcgg
    11341 gagctttccc tccgcaagaa cctcccgctg ccggcctgga gctacggcgc gaacatgctc
    11401 ctctggcagc cctacgcgtt cgccctcaag gggctcctga accgcgtgaa cccctaccgg
    11461 acggcctacc tgaagcgcct cgtgacggac cgccggagcg ccatcattca cttgggcggg
    11521 gccgagtttc tcgaaagccc cgaggcggcg gacgccctca accaggtgct ggaggcccag
    11581 agggggcttg acgtgcccgt ctacatcgtt ccggagctgg tgacctacgg ccggaggcgg
    11641 gagacggagc gcgagagcgt tctcaacatc ctcttcggac aaacggaaaa cacggggcct
    11701 tggctcaggg tcatcacctt cctccgctac gccaataagg cccaggtgat cgccgccgag
    11761 ccggtgaacg tggccgaatt cctggcggag cacgagggcc tgcccgcggc cgagcagtgc
    11821 cgccgcctgc gccaggaact catcgagcgg atcgacgcgg aaaagacgac catggtcggc
    11881 cccgtcctca agtcccgcgc ggagatcaac aacctcgtcc tcaaggatga ggacctcctc
    11941 cacttcatgg acgagcacgc ccgccgaacg gagaagggct tcgccgccgt ccggaaggag
    12001 gcgcggcgct acctgaagga gatctcgtcc gactaccgcg aaatgttcgt ggagatctgg
    12061 gacaagttcc tgacctggct gtggaacgac atctacgacg gcgtggtcgt cgatcgggag
    12121 gggatggccc gcctccgcga catttcgagg cggatgccct tcgtcatcat cccctgccac
    12181 cggagccaca tcgactatct gttgctgtcg tacgtgttct acaagaacaa catccagctt
    12241 cccttcatcg cggccgggac gaacctgatg ttctggccgc tgggggcgat cttccgcaag
    12301 tccggtgcct tcttcctgcg gcggagcttc aaggggaacc cgctgtacgc cgaggttttc
    12361 gccaagtacg tcaagaccct gctgcgggag ggcctgccga tcgagttctt cattgaaggc
    12421 ggacgcagcc ggacgggcaa gatggtgatg ccgaagtacg ggctgctgtc catcatcatg
    12481 cagtcgttca tcgagggctg ctgccaggac ctggccgccg tccccgtcta catcggctac
    12541 gaccgcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ggaggacgag
    12661 atcccggtgg aggacatcga ggaggtcgtc tactccctcg aggacgacaa gcggctgaac
    12721 ctggagtatt acaagaacaa catcctccac tacttcctgc cgatcgcctt cgtcgccgtc
    12781 tcggtcctgt cgagccggga ggacctgatc gccttttcac ggatcctcga ggactacggg
    12841 ttcttcaagc gcctgttccg ccatgaattc atcttcgacg acgaccggac ggatctggac
    12901 gaggtcaacg aggttctgac ctacctgcat gaccggggga tgattgccgg cgaggaacgg
    12961 ggcgaggagg cgctgatcga gatcaaggga cgcgggcgga cgaacctgag ggcctacgcc
    13021 gggctcatcc acaactatct ggagtcgtac tgggtggtgg tccgcgggtg cgcccacctg
    13081 aaggccggcg aggttccgga gcgggaattt ctgcggcgtc tccagacgct ggggctcaag
    13141 atgttcaaga agggggagat cacccgggcg gagtcgctct cccaggcgaa ttaccagaac
    13201 gccctccggt tcctccggga gcggggcgtc ctggtggcgg aggagcgacg ggacaagggt
    13261 gagaagcgcg aaacccgcta cctgtcgctc gccgaggatc gcgccccgct ggaggccctc
    13321 cgccgccgcc tcttccagtt catgtaaaaa aatagggacg tcgccctatg tctttgccgg
    13381 aaccggtcgg caggcgaagg gcgccggaaa aaacgggtga cgtccctatt ttcccatttt
    13441 ccttattttt ccccttgtta atccgcgcgt ttaaaaatag ggcgacgtcc ctattttttg
    13501 tggacggagt agagggccgc ggggccgcgg tagatggccg cgtcgccaag gtcctcctcg
    13561 atcctgagga gctggttgta tttcgcgagc cgctcgctcc gggacaggga accgctcttg
    13621 atctgcccgc agttcatccc tacggccacg tcggccatga agctgtcctc cgtctcgccc
    13681 gagcggtgcg atacgacggt cgtgtagccc gcctcctttg ccatctggat ggtttccagg
    13741 gtttccgtca gcgtcccgat ctggttgagc ttgatgagga tggagttggc gattcccgcg
    13801 tcgatgccgc gcttcaggcg ctgggcgttc gtcacgaaga ggtcgtcgcc catgatctgg
    13861 acccgaccgc ccaaggcgtc cgtcaggagc ttccagccgg cccagtcgtc ctcggccagg
    13921 ccgtcctcga tcgagacgat ggggtacttc ttcaccaggg cggcgtagaa cgcgatcatc
    13981 tcctcggccg tcttgacggg cttcttctcc gcggcgaggc ggtacttgcc gccggcgtag
    14041 aaggaactgg cggcggcgtc gagggcgatg gcgacgtcct ttcccggccg gtagccggcc
    14101 tggacgatcg cctctacgat cacttccagg gcctcctcgt tggacttcag gttcggggcg
    14161 aagccgccct cgtcgccgac ggcggtgttg tagcccctgg tcttcaggac ggccttcagt
    14221 cggtggaagg tctcggcggc catgcgcagg ccctccgcga agctcttcgc ccccacgggc
    14281 atgaccatga actcctggat gtcgacgttg ttgtccgcgt gctgaccgcc gttcaggatg
    14341 ttgcacatgg gtaccgggat gtccttcgcg ccgacgccgc cgatgtagcg gtacagcggc
    14401 aggtccaggg cctcggccgc cgcgcgggcg caggcgaggg agacgccgag gatggcgttg
    14461 gcgccgagat tccccttgtt gggcgtgccg tccagggcga tcatcgtccg gtcgatctcc
    14521 cgctgccgca ggcagtcaag gccgatgatt tcagggccga tcttctcgac gacgttccgg
    14581 acggccttcc ggacgccctt gccgccgtag cgcttcttca cgccgtcgcg caattcgagc
    14641 atctcgtgtt cgccggtgga ggcgcccgac gggacggagg cccgcccgac gatgcccgac
    14701 agcagggtga cctcggcctc gatcgtgggg ttgccccggg agtcgaggat ttctcgagcg
    14761 tgtacctgga tgatgtccgt catatgccct cctcgtgcct gcatggcgat tggcgtttgt
    14821 ccgcggatgc cgacagggcg ccgccccggt tccgccgtgt ctccgccgga cccggaattc
    14881 gcggggtcct gtcggtgcgc acggggggcg actttcctgg agactcttag cgtaaattcg
    14941 tacaatttac aatctgagat cgcaggcggc ggccggattc cccttgatcg gcggggagaa
    15001 gtcgggtagg atcgagacag gattcggggc cgtccccgga gggccgacgg agtagctgtc
    15061 ggcacagcgc gggatgccgg gacttcgtgc aaccggcccc gttacgaacc gcttcgaaac
    15121 ggggcctttt tcaccaaggc ctccggacgg gacgcatcgt ggaagagaaa agaacgagac
    15181 tccacctcca cctgaagaag tggctggaaa aggccgtcgg ggactacggc atgatcgcgg
    15241 cgggcgaccg cgtgctcgtt ggcgtctccg gaggcgtcga cagtttcgtc ctgctgggcc
    15301 tcctgaattc gcccatgatc ttcacgccgc ccttttcgat tctcgcggcg cacattgatc
    15361 tggggttcgc cccaaagggg gagggcgcgg cccgggtcgc cgcccgcctg gcggagacgg
    15421 ggatccccct cgtttccgaa cggacggata ttgggccgct tgcccacagc gaggtcaacc
    15481 gcaagaaccc ctgcttcttg tgctcgcgcc tgcggcggcg gcgcctcttc gagatcgcgg
    15541 cggccgaggg atgcacgaaa gtcgcgctcg cccatcaccg ggacgacatc atcgagacgc
    15601 tcttgatcaa cctgttcttc ggacgggaga tcagcacgat gacgccgaac cagcccctct
    15661 t
//
LOCUS       DAOOLJ010000038        15621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_3992_length_15621_cov_17.851728, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000038 DAOOLJ010000000
VERSION     DAOOLJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3992_length_15621_cov_17.851728"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLW16_07145"
     CDS             complement(<1..356)
                     /locus_tag="PLW16_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70717.1"
                     /translation="MMEEGIVKRLSFLDRYLTLWIFLAIGLGVGIGFFLPGLSKFITG
                     LQVGTTSIPIAVGLILMMYPPLARVKYEQMGKVFENKRMLAISLLQNWVIGPLVMFVL
                     AVIFLHNYPEYMVGVIL"
     gene            complement(398..640)
                     /locus_tag="PLW16_07150"
     CDS             complement(398..640)
                     /locus_tag="PLW16_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB70718.1"
                     /translation="MDIKILGPGCVKCRQVEALVKEAVAETGKEAQIEHVTEFRKIAD
                     YGVFATPSVVVDGEVKCVGKIPKKEEIKAWLDEAAR"
     gene            complement(709..1218)
                     /locus_tag="PLW16_07155"
     CDS             complement(709..1218)
                     /locus_tag="PLW16_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB70719.1"
                     /translation="MTENEVAKIRVNGHPIGLVGLAEAMEEIARKRTDETDEALREAL
                     IDRLSADNYIPRSARPAYGQALLREFKKFLGEAVEDEPAAGLSVLILGASCTRCDQLE
                     RSVLDMLSEMNLPAAVDHVTAPLEIAKYGVMGTPALVIGGKVVWIGSVPPKHRIKQWL
                     LEAQGASPA"
     gene            complement(1286..2458)
                     /locus_tag="PLW16_07160"
     CDS             complement(1286..2458)
                     /locus_tag="PLW16_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB70720.1"
                     /translation="MKETTKLLLIAAGFLVAYYIPWDHEVIRRSGLEAFLMLQDYARQ
                     HVLTCLIPAFFIAGAIAVFVSQASVLKYFGAQANKFLSYSVASVSGTVLAVCSCTVLP
                     LFAGIYTRGAGIGPATAFLYSGPAINVLAIVLTAKVLGWQLGLARAVGAVIFAIVTGL
                     LMAWLFRKDDANRTAGQIYLPEEDGKARSLWQEAVYMLTMVLILVFAAFARPAADDGS
                     LWKAIFAAKWYITGALLIALAVMLKAWFVKEELTSWVEATWGFMKQIFPLLLVGVLAA
                     GFLLGRPGHEALIPGHYIVDLVGGNSIWANLFASIAGALMYFATLTEVPILQGLIGSG
                     MGRGPALALLLAGPALSLPNMLTIAGIMGVKKTLTYCAIVVVLSTIAGMAYGWLAG"
     gene            complement(2559..2909)
                     /locus_tag="PLW16_07165"
     CDS             complement(2559..2909)
                     /locus_tag="PLW16_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB70721.1"
                     /translation="MQEFIKVMKALSDPNRVRLLKMLQRRVMCVCEIQAVLGIAQPTV
                     SKHLKLLEGAGLVSHRKEGLWVNYALTDGARSPYAASLLGNLRHWLDDAPEIQDLFKR
                     SKNIRREDLCHTRH"
     gene            3117..4553
                     /locus_tag="PLW16_07170"
     CDS             3117..4553
                     /locus_tag="PLW16_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB70722.1"
                     /translation="MDKPAIDLGVFLQPRSVAVIGASDKPGSWGSFMMEGLAARRYPG
                     TIYPVNRRAGTVFGHPAFTDVRDIPGEVDLAVMAVPEAFVEETIRACGEKGVRGMSII
                     TAGFGEAVRGGRERELAFARLAHGYGMRLLGPNISGTFNLHAGFNAAASPAEHLYPTK
                     LAAVCQGGFAFYDLLAAGSFRKMGVGRFVHTGNECDLTVTDFLEYFGADPEVPAILTY
                     LETVRDGERFLEVARRVTRKKPVIAFKAGRSAGAARAAQSHTGALAGRAEVFVGFLRQ
                     AGVITVPTMDVMLPLAHALVEHPPLKGPRIGIVTIGGSWGVALADALEEEGLCVPELS
                     TELQEKLRALDMPPRASTRNPIDLGAAGMVSLPDRRQTLGRLLLESGEVDALVVHGLG
                     RPGRITEMRSEDWKAFLRFEKWTVRQFVDLERETGLPVLVGAHFAPWESQLVWDLNEK
                     GIRIHNRLDEIARVLAGMLRDDHRGPGG"
     gene            complement(4668..5414)
                     /locus_tag="PLW16_07175"
     CDS             complement(4668..5414)
                     /locus_tag="PLW16_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB70723.1"
                     /translation="MTVLLRLAKTLWIYFWAILSSLLLFVPICLAAFFSRTGNLAFNL
                     SQLWAWIMLKVTCVRVMTRGRDKIRQGQSYIIIANHASVYDILALVTGLRTQYRWIIK
                     KELLRVPLFGYALYASRNIFIDRADPARAITSIHKALDRLPAGVSVLFFAEGTRSPDG
                     RIRPFKKGGFVMAVEKGLPILPVTINGSRGILPKKSVAFNPGTVEVVVGDPIATEGED
                     RDHLEALIERTRNVIIANYRSGEQALLTGH"
     gene            5608..6417
                     /locus_tag="PLW16_07180"
     CDS             5608..6417
                     /locus_tag="PLW16_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPB70724.1"
                     /translation="MNELNRYRQFGRKIEAFLRPASFPFAIKLIRTEEEIPPGAKRPS
                     VDFRHQNFVCQNFKIARNYGWTLAVTEKDCNCKIARTVYGWDPLTPEVQQFAVQFTVG
                     LYSKDAATSNKWTDTLYMLKENYAGIVISPLARTKVVPDVIQIYGLPAQIMRLVQGYL
                     YMEGGTMQFTSSGRAGSCHEGVIKTIQLDAPQVVLLGNGDRVWGGAEDAEIMFSSPRS
                     KLALLVEGLEATHQAGLRYPIPKYMNYSPGFQDEFEQRAIERAGGTLVKKR"
     gene            complement(6482..7444)
                     /locus_tag="PLW16_07185"
     CDS             complement(6482..7444)
                     /locus_tag="PLW16_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB70725.1"
                     /translation="MEYPGQMGRRRFLAGGAAMAASLALPGVSSARIVKATGPLKKKK
                     PESALVVWYSQTGHTERYGRLIARCWGKQGLSVDAVPMKRADRSLLARYDLIAVGTPV
                     QYLDVPPNVQDWLAGVPSIPGTGIVGYVSFGGKGDGQHNAAVTLLDTLTRRGGVPLGM
                     GTFGNMSAYPPTWSMGYEARTLAFRDKPDQATYAQVRKLAADVLGRYGRGRGIEVKDE
                     LSVRGLFKGGISRGIAKATIGRHEIDPGRCIHCGTCVDRCPVGAIDLAVPRVDTRRCI
                     LCFGCLNNCPTGAHEMTVFGRKIYSFAELLRRNGITLLEPPELV"
     gene            complement(7616..7816)
                     /locus_tag="PLW16_07190"
     CDS             complement(7616..7816)
                     /locus_tag="PLW16_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB70726.1"
                     /translation="MAEGTVKWFNEKKGYGFIAKDEGGDVFVHFSAIQGDGFKTLAEG
                     QRVSFEIEQGQKGPSAANVRAI"
     gene            8120..9064
                     /locus_tag="PLW16_07195"
     CDS             8120..9064
                     /locus_tag="PLW16_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB70727.1"
                     /translation="MNASMEIQHPKILEIMACLSGYLYGKDLALKLALITCCSRGHLL
                     IEDLPGLGKTVLALSIARVLGLTFGRIQCTSDLLPTDITGLSIYNKNTAEFEFRPGPV
                     FNHIVLVDEINRATPKTQSALLEAMGEKQTTIEGKTYLHPQPFFVIATQNPVEHFGTF
                     PLPESQMDRFMMQIRIGYPSGDEEKEILKQGSSRRELDRIDPLVSRDEVVEMHRRINT
                     EVHVSEKILGYILDIVAASRHHPYIRAGISTRGALAVTASARTYAFFCGRDFVIPEDI
                     RELAPFTLPHRLLLKEEYESLDKKELVQSILDTVKVPA"
     gene            9094..9948
                     /locus_tag="PLW16_07200"
     CDS             9094..9948
                     /locus_tag="PLW16_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB70728.1"
                     /translation="MALTLFLGIAAINTGNNLVYMIVAALLSFLLISGFYGKRNLSRL
                     IVDVTFPEEVFARTETLVTVVLVNRRRYLPAFLMRVSLGETSVLFPFLEAGHRATRPA
                     RLTFAGRGHQAVSPRYLASSFPFRFFIRYRRLEGDVTALVYPEPKPGSVGREEGRSRS
                     RPSESGLDLSGAGTDLLSIRPYLVGDPLKYIHWKASARTDRLQTKEFTLPAMPPLVLD
                     MARVPGPLEGRLSILTFWVLALFRKGVPVGLKMGEILYPPVHSPHQKHLLLRALALCG
                     HEGEAHEG"
     gene            9938..11863
                     /locus_tag="PLW16_07205"
     CDS             9938..11863
                     /locus_tag="PLW16_07205"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HPB70729.1"
                     /translation="MKVEGFLKILTYTLTLTGFVSVAAHVGATFVAGFVALMLPAAWR
                     DWRRPFVVPRWILNALAVAMVIVTFLRLRIDNIVVPAVDILLVMIALKFMEDKQPRDY
                     FQIFVLSLLLLAGSSLLSLDMIFLAYLIAFLFLLSSATVLLAYFAEDPALVMPRRTLI
                     AIALKSLLIPLVALPVTVILFVLLPRTSHPFFDFLNRTGPATSGFSDHVRLGGVADIQ
                     EDTTIAFRVAMGRLDEDALYWRGIVFDQFDGRTWKTLRTPPATTFHISAPTRRVRYTV
                     YLEPSDNRYLFTLDKPVFVSLRNARGGGNLVYTLPDPILRKIRYEGVSVPADDFIPAH
                     ENPRAYLDLPPGLEDIRALTASLTRGLAPLPAVRALHGYLHHGPFRYALSDLPRSSRP
                     LQDFLFRHRYGNCEYFASAMTVMARSAGVPARLVGGYRGGYYHDLGGYYAVLQKNAHV
                     WTEVWLEGHGWLRVDPTPAALLIATSVWQGGFGFRVRMIFDLLEYYWNINVIAYDLQK
                     QFRLLTTLERTLRNPDWHLSLDPGQRLWGGGALLSLMALAGVLLLRRLKWPTPEERLL
                     ARFHRRLARLGHTRHPSEGLEAFIARLPADVPRRQAEIFVAEFERFYYKDIAIPKHER
                     ARLAALIRSISGRRPEG"
     gene            11986..12306
                     /locus_tag="PLW16_07210"
     CDS             11986..12306
                     /locus_tag="PLW16_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB70730.1"
                     /translation="MPFINLASLAEREMSPGFRARFVHTDHMTLAYWHIRAGASLPDH
                     AHPHEQVTHLIEGAFSLTVDGETRTLAPGGVAVIPPGVPHSGRAETDCVIIDVFYPPR
                     DDYR"
     gene            12469..13239
                     /locus_tag="PLW16_07215"
     CDS             12469..13239
                     /locus_tag="PLW16_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPB70731.1"
                     /translation="MAKILCIANQKGGVGKTTTAINLAASIAAAERRTLLIDCDAQGN
                     ASTGLGVSREAIREQNLYQALIGKRPLKDVIVGTMIPQLDIVPADQDLIGIEVEFVPL
                     PDREKKLRTLVRELDTPYEFIILDCPPSLGFMTLNALVAADALIIPLQCEYFAMEGLG
                     HLLNTLRLVKAHLNPSLQLGGILLTMFDTRNLLSHRVSEDVRRHFGSRVFQTYIPRNV
                     RLSESPSHGLPILLYDIRSRGALSYMDLAKEILANGRR"
     gene            13240..14085
                     /locus_tag="PLW16_07220"
     CDS             13240..14085
                     /locus_tag="PLW16_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPB70732.1"
                     /translation="MPPKQSLGKGLGAMFPDLLTDASNRPAFITCGIEALVPNRFQPR
                     RNFRDTEQHQLVQSIRQNGIVQPILVRRVDEGYEIIAGERRWRAAQKAGLSEVPVIVR
                     EATDRDLAEVSLIENLQREDLDPIEEANAYQTLMETFGLSQEEVSSRVGRDRSTVANT
                     VRLLKLPGEARQAIIDRKISAGHGRALLALDTLGEQRRILAQVLKNCLSVRETEALVQ
                     RSKRPLPPATAKRKDPYVADLERTLSRTVKSPVKIHQGRKKGYIQIAFQNTEELNRLS
                     RLLLG"
     gene            14134..15501
                     /gene="dnaA"
                     /locus_tag="PLW16_07225"
     CDS             14134..15501
                     /gene="dnaA"
                     /locus_tag="PLW16_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPB70733.1"
                     /translation="MWVVEILWEKSTGILKEKISSQNFETWIKPVRISAMDGQQIQLT
                     VPNKFFRDWLLDNYVTLIREALKAASGVDLQIDFRIGRDAKSPAGGASAPSGAPAARS
                     RTARVKAPSRNHPTLNANYSFDRFVVGSCNQFAHAAAVAVAEQPAKQYNPLFIYGGVG
                     LGKTHLLNAIGLQMLALYSQRNVAYVSAEEFMNELINSIRYDKMPQFREKYRHIDCML
                     IDDIQFIAGKERTQEEFFHTFNTLHDGGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLI
                     ADIQPPEMETKIAIIEKKAQENNINLPHNAANYIASHAESNIRELEGFLIRIGAFSSL
                     TGREIDLDLVREVLKKILKQREKEEISIDDIVKTVAGKLNLKIADIKSPKKNKNMVLG
                     RQVAMFLARQMTPASYPDIGDKIGGKDHSTVIYACQKISRLMETDTKLKKTVQDIEEV
                     LQSRS"
BASE COUNT         3001 a         5080 c         4558 g         2982 t
ORIGIN      
        1 aggatgacgc cgaccatgta ttccgggtag ttatgcagga agatcaccgc caggacgaac
       61 atgaccagcg gaccgatgac ccagttctgc aggagcgaga tggcgagcat ccgcttgttc
      121 tcgaagacct tgcccatctg ctcgtacttc acccgggcca ggggcgggta catcatcagg
      181 atcaggccga ccgcaatggg gatcgaggtc gtccccacct gcaggccggt gatgaatttc
      241 gacagccccg gcagaaagaa cccgatcccg actcccaggc cgatggccag aaagatccac
      301 agcgtcagat accgatccag aaacgacaac cgcttgacga tcccttcctc catcatttgg
      361 acctcctcgt gcagttcagg ccggttcggc cggccggtca gcgcgccgcc tcgtccagcc
      421 aggccttgat ctcttccttc ttcgggatct tccccacgca tttcacctcg ccgtccacga
      481 ccacggaggg ggtggcaaag acgccatagt cggcgatctt cctgaactcc gtcacatgtt
      541 cgatctgtgc ctctttgccc gtttcggcca ccgcctcctt gaccagcgcc tcgacctgac
      601 ggcacttgac gcaaccgggc cccaggattt tgatgtccat cgccttgcct ccttcttacc
      661 cgaaaatcat ggtcgattca ttgtcgatct gatttttttc caaaacactt acgccggaga
      721 ggccccttgc gcctccagca gccactgttt gatccgatgc ttcggcggca cggacccgat
      781 ccagacgacc ttgccgccga tcaccagggc cggcgtgccc atgacaccgt atttcgcgat
      841 ctccaggggc gctgtcacgt ggtccaccgc ggcgggcaga ttcatctcgc tcaacatatc
      901 caggacggac cgctccaact gatcgcatcg ggtgcagctc gcccccagaa tcaggacgct
      961 caaacccgcc gcaggttcat cctcgacggc ctcgcccagg aatttcttga attcgcgcag
     1021 cagggcctgg ccataggcgg gtctggcgct tctcgggata tagttgtccg ccgataggcg
     1081 gtcgatcagc gcctcccgga gggcctcgtc cgtttcgtcg gttcgcttcc gggcgatctc
     1141 ctccatcgcc tccgccaatc cgacaagacc gatcggatga ccgttgaccc ggatttttgc
     1201 cacctcgttt tccgtcacga tatccccgct tatcgaagaa ttttcattgg ccggattcag
     1261 gccgacgttc cgggactggc cggccctatc ccgccagcca cccgtaggcc atccccgcga
     1321 tggtggaaag gaccacgacg atggcgcagt aggtcagggt cttcttcacg cccatgatac
     1381 cggcgattgt cagcatgttc ggaagcgaca gcgccggtcc agccaggagc agcgccaggg
     1441 cgggtccccg gcccatgccc gaaccgatca gcccctggag gatcgggacc tccgtcagag
     1501 tcgcgaagta catcaaggcc cccgcgatcg aggcgaagag gttcgcccaa atggagttcc
     1561 cgcccacaag gtcgacaatg taatgccccg gaatcagggc ttcatggccg ggacgcccca
     1621 acaggaatcc cgccgccagg accccgacga gcagcagcgg aaagatttgc ttcatgaacc
     1681 cccaggtcgc ctcgacccag gacgtcagtt cctccttgac gaaccaggcc ttcagcatca
     1741 ccgcgagcgc gatcaggagc gcaccggtga tataccactt ggcggcgaaa atggctttcc
     1801 agaggcttcc gtcgtcggcc gcaggcctcg cgaatgctgc gaagacgaga atcaaaacca
     1861 tggtcagcat atagacggcc tcctgccaga gactgcgggc cttgccgtcc tcttcgggca
     1921 gatagatctg cccggccgtc cggttcgcgt cgtccttccg gaacaaccag gccatcagca
     1981 gacctgtgac aatggcgaaa atcaccgcgc cgacggcccg ggcgagtccg agctgccaac
     2041 ccaggacctt cgccgtcaga acgatggcca ggacgttgat cgccgggcct gagtatagga
     2101 aggccgtcgc ggggccgatc cccgcgcccc gcgtgtagat cccggcgaag agcggcagga
     2161 ccgtgcacga gcaaacggcc agaaccgtcc ccgagacgga agcgaccgaa taggagagaa
     2221 atttattggc ctgcgcgccg aagtacttca ggaccgacgc ctgcgagacg aagacggcaa
     2281 tcgctccggc gataaagaag gcggggatga ggcaggtcag aacgtgctgc cgggcgtagt
     2341 cctggagcat caggaaggcc tccaggcccg accggcggat cacctcatgg tcccacggaa
     2401 tataatatgc gaccagaaaa ccggccgcga tgagtagcag cttggttgtc tctttcatga
     2461 tttcctcttt gtacatgtgt gcatgtactt tacacgccat ttacgacggg gatcggcgcg
     2521 ctccccatca ccggacggca attcagtgcc gctggagatc agtgacgggt gtgacagagg
     2581 tcctctcgac gaatattctt gctccgcttg aacagatctt gaatttccgg ggcgtcatcc
     2641 agccaatgtc tcagattccc caggaggctc gcggcataag ggctacgcgc accgtccgtg
     2701 agcgcgtagt tgacccagag cccctccttc ctgtggctga ccagtcccgc accctccagg
     2761 agcttgaggt gcttcgagac cgtaggctgg gcaatcccca agacggcctg gatctcgcaa
     2821 acgcacatca cgcgcctttg gagcatcttg agcaaccgca cccgattggg atcggagagg
     2881 gccttcatga ccttgatgaa ttcctgcatg gttcatctct catattgctt tccggcaata
     2941 taaggatgcg ccgatcccgt gtcaaggaaa atcgcgccag gaatctacac gccgatccgg
     3001 gccatcccct ttcagccatc tgacgccccg ccggaacgcc tccggcatgc gtgaacttga
     3061 cccgccccac caggggtggt acagtgccgg ccacactgac gaacaaaggg gggatcatgg
     3121 ataagcccgc gatcgatctc ggcgttttcc tccaaccccg ttccgtggcc gtcatcggcg
     3181 cttcggacaa gcccggctcc tggggttcgt tcatgatgga ggggctggcc gccaggcgct
     3241 atcccggcac gatctacccg gtcaaccggc gggccgggac ggtcttcgga catcccgcct
     3301 ttacggacgt ccgggacatc cccggcgagg tggatctggc cgtcatggcg gtgccggagg
     3361 cgttcgtcga ggagacgatc cgcgcctgcg gcgaaaaggg cgtccgggga atgtccatca
     3421 tcaccgcggg tttcggcgaa gccgtccggg gcgggcgcga gcgcgaactg gccttcgccc
     3481 gcctggccca cggttacggg atgcgtctcc tgggtcccaa catcagtgga accttcaacc
     3541 tccatgccgg attcaacgcg gccgcctcgc ccgccgaaca cctctacccc acgaagctgg
     3601 cggccgtctg ccagggcgga ttcgccttct acgatcttct cgccgccggg tccttccgga
     3661 agatgggggt gggccggttt gtgcacacgg gcaacgagtg cgatctcacc gtgaccgatt
     3721 tcctggagta cttcggcgcc gatccggagg tgcccgccat cctcacctat ctggagacgg
     3781 tccgggacgg ggagcgcttt ctcgaggtcg cccgacgcgt gacgcggaag aaacccgtga
     3841 tcgccttcaa ggccggccgg tccgcgggcg ccgcccgcgc agcccagagc cacaccggcg
     3901 cactcgccgg ccgcgcggag gtcttcgtgg gattcttgcg tcaggcgggc gtgatcaccg
     3961 tgcccaccat ggacgtgatg ctgcctttgg cccatgccct agtcgaacac cccccactga
     4021 aggggccgcg gatcggcatc gtgaccatcg gcggctcctg gggcgtggcc ctggcggacg
     4081 ccctggagga ggaaggcctt tgcgttcctg agctgagcac tgaactgcag gagaagcttc
     4141 gcgccctcga catgccgccc cgggcctcga cgcggaaccc catcgatctg ggggccgccg
     4201 gcatggtgtc tctgccggat cggcgtcaaa cgctcgggcg gttgctgctt gagtccggcg
     4261 aggtcgacgc cctggtcgtg cacggcctgg gccgaccggg acggatcacg gagatgcggt
     4321 ccgaagactg gaaggcattc ttgagattcg aaaaatggac ggtccggcag ttcgtcgacc
     4381 tggagcgtga aacgggcctt cccgtgctcg tcggggcgca tttcgcgcct tgggagagcc
     4441 agctcgtctg ggatctcaac gagaagggaa tccggatcca caaccgtctg gacgagatcg
     4501 cccgcgtcct ggccggcatg ctccgggacg atcaccgggg gccggggggc tgatcgtctt
     4561 catcgccacc cgggattcga cggacggggg caccttccgc ccggcacggc atccgattcg
     4621 cgcttcccgg aggggggttc cgtccgccgg gtcgatcggg cggctcgtca gtgaccggtg
     4681 agcagagcct gctcaccgga gcggtagttg gcgatgatga cgttgcgcgt ccgttcgatg
     4741 agggcctcca ggtggtcacg gtcctcgccc tctgtcgcga tggggtcgcc gacgaccacc
     4801 tccacggtgc cggggttgaa ggccacgctt ttcttcggga ggatcccacg gctgccgttg
     4861 atggtcacgg gcaggatggg caggcccttt tccacggcca tgacgaagcc gcccttcttg
     4921 aacggccgga tgcggccgtc cggtgaacgg gtgccttccg cgaagaacag gacgctgacg
     4981 cctgcgggaa ggcggtccag tgccttgtgg atgcttgtga tggcccgggc cggatcggcc
     5041 cggtcgatga agatgttgcg agaggcgtac agggcatagc cgaagagggg aaccctcaga
     5101 agctcctttt tgatgatcca gcgatactgg gtgcgcagcc ccgtcacgag ggccaggatg
     5161 tcgtaaacgg aggcgtgatt ggcgatgatg atataggatt gtccctgccg gatcttgtcc
     5221 cgtccccggg tcatgacccg gacgcatgtc accttgagca tgatccaggc ccagagctgg
     5281 ctcaggttga acgccagatt ccccgtacgg ctgaagaagg cggccaggca gatgggaacg
     5341 aagagcagaa gcgaggacag aatggcccag aagtagatcc acagcgtttt ggccaggcgc
     5401 agcagaacgg tcatctcttc cctccggcag tgggatgaac ggatcgacgg gtgaattcat
     5461 ataccataat tttcttttgg ggctataggg gaatcgggcg tttcgctacg cgcgggtcac
     5521 cgcccggcgg ggcgcgggtc ctgttcctgg gtcaggtcac ggatgagcgc tttaatttcg
     5581 aaaagcatga tgaagggggg ctcacgaatg aacgaattaa accgatatcg gcaattcgga
     5641 cggaaaatag aggcgtttct cagaccggcc tctttccctt tcgcgatcaa attaatcagg
     5701 acagaagaag aaatcccgcc gggagccaaa cgtccgtcgg tggattttcg gcatcagaat
     5761 ttcgtctgcc agaacttcaa aatcgcgcga aactatggat ggaccctcgc cgtgaccgag
     5821 aaagattgca attgtaaaat tgcacgcacc gtttacggtt gggatccact cacccccgag
     5881 gtccaacagt tcgcggttca attcacggtg ggattatatt ccaaggatgc cgcaacgtcg
     5941 aataaatgga ccgacacctt gtacatgctc aaggagaact atgcgggcat tgtgatctcg
     6001 cctttagccc gcacgaaagt tgtgccggac gtcattcaga tttatggatt gcccgcacag
     6061 attatgcgtt tggttcaagg ctatttatac atggaaggcg gcacgatgca atttacgtct
     6121 tccggtcgag cgggatcctg tcatgaaggc gttatcaaga cgatccaact cgatgcaccg
     6181 caagtggtct tgctgggaaa cggagacagg gtgtggggtg gcgctgaaga cgctgagatc
     6241 atgttttcat cccctcgcag taagttagcg ctccttgttg agggattaga ggcaacccac
     6301 caggcgggcc tcagataccc cattccgaaa tacatgaact attcgcctgg atttcaggat
     6361 gaattcgaac aacgggcgat tgaacgagcg gggggcacgc tcgtcaaaaa aagatgaaga
     6421 ccatctgcga tccatcggtg cacgaatcgc tcggcggcgt tggacgcggg gaggtgcgat
     6481 ctcaaaccag ttcggggggc tccaggaggg tgatgccgtt gcgcctgaga agttcggcga
     6541 agctgtagat cttcctgccg aagaccgtca tctcatgcgc accggtcgga cagttgttca
     6601 gacagccgaa acacaggatg cagcggcggg tatccacgcg ggggacggcc aagtcgatgg
     6661 cgcccacggg acaccggtcc acacaggtac cgcagtggat acagcgcccg gggtcgatct
     6721 cgtgccgccc gatcgtcgcc ttggcgatcc cccgggaaat gccgcccttg aaaagtcccc
     6781 tgacgctcaa ttcgtccttc acctcgatgc cccggccgcg cccgtagcgg ccgagcacat
     6841 cggccgctag cttacgcacc tgcgcatagg tcgcctggtc cggcttgtct cgaaacgcca
     6901 gggtgcgcgc ctcgtagccc atggaccagg tcggcggata ggcgctcatg ttgccgaacg
     6961 tccccatgcc caagggaacg ccgccccgtc gggtgagggt gtcgagaaga gtcacggccg
     7021 cattgtgctg gccgtcgccc ttgccgccga aactgacgta ccccacgatg cccgttcccg
     7081 ggatggacgg cacccccgcg agccagtcct gcacgtttgg gggaacatcg agatattgaa
     7141 ccggggtgcc gacggcaatc aggtcatacc gggcgaggag ggaccggtcg gcccgcttca
     7201 tcgggacggc atcgaccgaa aggccctgct tcccccaaca gcgggcgatc aggcgcccat
     7261 agcgttccgt atgacccgtc tggctgtacc agacgacgag ggcactctcg ggttttttct
     7321 ttttcagggg acccgtcgcc ttgacgatac gggcggatga cactccggga agggccagcg
     7381 aagccgccat ggccgcacca cccgccaaga acctgcgtcg gcccatctgt cccggatatt
     7441 ccacgcgttc aatccctgga aggcaaaaag acgcaccgcg ttcccaaacc ggaacacgtc
     7501 gtctcgatcg gcgatgcttc accggcgccg aagggcgccg acgggaagga cgccgggggc
     7561 accccccggc tgaagggcgc ccctggctca aacaaaacct gaaaccgttc cggctctaga
     7621 tggcccggac gttggccgcg gacggccctt tctgaccctg ctcgatctcg aagctgacgc
     7681 gctgaccctc ggccagagtc ttgaatccgt ccccctggat cgcgctgaaa tggacgaaaa
     7741 cgtccccccc ttcatccttt gcgatgaaac cgtacccctt cttctcgttg aaccacttta
     7801 ccgtgccttc tgccatgctg cctaaccctc tcctgtcagt gtgtttgcgg gcggcaataa
     7861 aaaaaccacc ttgacccgcg agtgcatggt ggttgttcca ttgcccttca atacgtcttc
     7921 ctttaaatcc cgtctggcgg catagagaaa gccgcattgt tcaaaaaacc atgtcctcct
     7981 taaggtgtgt tggaaaggat gtcaagtctt ttttcacgct cccgccatcg ggacgcgggg
     8041 gaccaaaacg acctcatttt gcctgtgatc cccccgcgtt ccctgcaaac ggacttggac
     8101 aacggccgga gaagatggta tgaacgcctc catggagatc caacatccga aaattctgga
     8161 aatcatggcg tgtctatccg gctacctgta cgggaaggac ctcgccctga agctcgccct
     8221 gatcacctgc tgctcccggg gacacctctt gatcgaggac ctgcccggtc tgggcaaaac
     8281 cgtccttgcg ctctccattg cccgtgtcct ggggctcacc ttcgggcgga tccagtgtac
     8341 gagcgacctc ttgccgacgg acattacggg cctgtccatc tacaacaaga acacggccga
     8401 gttcgagttc cgccccggac cggttttcaa ccacatcgtg ctcgtcgacg agatcaaccg
     8461 ggccaccccg aaaacccaga gcgcgctgct cgaggcgatg ggggaaaagc agacgacgat
     8521 cgagggcaag acgtacctcc acccccagcc gttcttcgtc atcgccaccc agaaccccgt
     8581 ggagcacttc ggcacctttc ccctgccaga gtcccagatg gaccgtttca tgatgcagat
     8641 ccggatcggc tatccttccg gcgacgagga aaaggagatc ctgaagcagg ggagcagccg
     8701 ccgggaactg gaccggatcg accccctcgt ctcgcgcgac gaggtcgtgg agatgcaccg
     8761 gcggatcaac acggaggtcc acgtctcgga aaagatcctg gggtatattc tggacatcgt
     8821 cgccgcctcg cggcaccacc cctacatccg cgccggcatc tcgacccggg gggcgctggc
     8881 cgtcaccgcc tccgcccgga cctacgcctt tttttgcggc cgggatttcg tcatcccgga
     8941 agacatccgc gaactggccc cctttaccct gccgcaccgc ctcctgctca aggaggaata
     9001 cgaaagcctc gacaagaagg agttggtcca atcgatcctc gacacggtca aggtcccggc
     9061 gtaatccaga tcacgcgtgc cggctggatc tacatggccc tgacgctctt cctcggcatc
     9121 gcggcgatca acacgggcaa caacctcgtg tacatgatcg tcgccgctct gctctccttc
     9181 ctcctgattt cgggcttcta cggcaagcgc aacctgagcc ggctcatcgt cgacgtcacc
     9241 ttccccgagg aggtgttcgc ccggaccgaa accctcgtca ccgttgttct cgtcaaccgg
     9301 cggcgctatc tgccggcgtt tctgatgcgc gtgtccctgg gggaaacctc cgtcctcttt
     9361 cccttcctgg aagccggaca ccgggccacc cggcccgcgc gcctgacctt cgccgggcgg
     9421 gggcatcagg cggtctcacc ccggtatctg gcctcctcgt ttcccttccg tttcttcatc
     9481 cgctaccggc gactggaggg cgacgtgacc gccctggtct accccgaacc aaagccaggg
     9541 tcggtcgggc gggaggaagg ccgttcccgt tcccggccgt cggagtccgg tctggacctg
     9601 tccggcgcgg ggacggatct cttgtccatc cgcccctatc tcgtgggcga ccccctcaag
     9661 tacatccact ggaaggcgag cgcccggacg gaccgcctgc agaccaagga gtttaccctc
     9721 ccggccatgc cgccgctggt gctcgacatg gcccgggttc ccggccccct tgaagggcgg
     9781 ctgtccattc tgaccttctg ggtgctcgcc ctgttccgga aaggcgtccc ggtgggcctg
     9841 aagatgggcg agatcctcta tccgcccgtc cattcgcccc atcagaaaca cctcctgctc
     9901 cgggcgctgg ccctttgcgg acacgaaggg gaggctcatg aaggttgagg gcttcctcaa
     9961 gatcctgacc tacaccctca ccctgacggg atttgtgtcc gtggccgccc acgtcggggc
    10021 caccttcgtc gccggtttcg ttgctctgat gcttccggcc gcctggcggg actggcgccg
    10081 gcccttcgtc gtgccccggt ggatcctgaa cgcgctcgcc gtggccatgg tcatcgtcac
    10141 ctttctccgg ctgcggatcg acaacatcgt ggtgccggcc gtggacatcc tgctcgtcat
    10201 gatcgccctg aaattcatgg aggacaagca accgcgggat tacttccaga tcttcgtcct
    10261 gtccctcctc ctgttggcag gctcctcgct gctgtcgctg gacatgatct ttctcgccta
    10321 tctgatcgcc tttctcttcc tcctgtcctc cgccacggtc ctgctggcct acttcgcgga
    10381 agaccccgcc ctcgtcatgc ctcggcgcac gctgatcgcc atcgccctca aatccctcct
    10441 gattcccctg gtcgccctgc cggtcacggt tattctgttc gtcctccttc cgcggacgag
    10501 ccaccccttt ttcgattttc tgaaccggac cggtccggcc accagcggct tcagcgacca
    10561 cgtgcgtctc ggcggcgtgg cggacatcca ggaggacacg accatcgcct tccgtgtcgc
    10621 catggggcgg ctcgatgaag acgcgttgta ctggcggggc atcgtgttcg accagttcga
    10681 cggacggacc tggaagaccc tccggacgcc acccgcgaca acttttcaca tctccgcgcc
    10741 gacccggcgc gtccgctaca ccgtctacct ggaaccctca gacaatcgct atctgttcac
    10801 cctggacaag cccgtcttcg tttccctccg gaacgcccgt gggggcggaa acctcgtcta
    10861 taccctgccg gacccgattc tccgaaagat ccggtacgaa ggcgtctccg tccccgccga
    10921 cgacttcatc cccgcccacg aaaatcccag ggcgtacctc gacctgcccc cggggctgga
    10981 ggacatccgg gccctgacgg ccagcctcac ccggggcctg gcccccctcc cggctgtccg
    11041 ggccctccac ggctacctgc accacggccc cttccgctac gccctctcgg atctcccccg
    11101 ctcttcccgg ccgctccagg attttctctt ccgtcaccgt tacggcaact gcgaatattt
    11161 tgcgtcggcc atgaccgtca tggcgcgttc ggcgggggtc cccgcccgcc tggtcggcgg
    11221 ctaccgcggc gggtattatc acgacctggg cggctattac gcggtccttc agaaaaacgc
    11281 ccacgtctgg acggaggtct ggctggaggg acacggctgg ctgcgtgtcg atccgacgcc
    11341 cgccgccctc ttgatcgcga cctccgtgtg gcaaggaggt ttcgggttcc gcgtgcgcat
    11401 gatcttcgat ctgctcgaat attactggaa tatcaacgtt attgcctatg acctccagaa
    11461 gcagttccgc cttctgacga ccctggagcg gacgctccgg aaccctgact ggcacctgtc
    11521 cctcgacccc ggacaacgcc tctggggcgg cggcgcgctc ctgagcctca tggccctggc
    11581 cggcgttctt cttctgcggc gtctgaaatg gccgacgccg gaggaacggc tccttgcgcg
    11641 tttccaccgg cgcctcgcac gcctcgggca tacgcgccat cccagcgaag gccttgaggc
    11701 gtttatcgcc cgcctccctg cggacgttcc ccggcggcaa gcggaaattt ttgtggcgga
    11761 gttcgagcgt ttctattaca aggatatcgc catcccgaag cacgagcgcg cccgcctcgc
    11821 cgccctgatc cgctcgatat cgggacgcag gccggaggga tgaccgggtc cgggcctgcc
    11881 ggttcgtttt cccggaccct tcgacggtcg gccctgcctc ccgtcgcgcc cgggaccttt
    11941 tgtccccgac cccggccctt tcaccctttg cccccaggag gtatcatgcc cttcatcaat
    12001 ctcgcgtccc tcgcggagcg tgaaatgtcc cccgggttcc gggcccgctt cgtgcatacg
    12061 gaccacatga ccctggccta ttggcatatc cgggccggcg cctccctgcc ggaccacgcc
    12121 catccgcacg agcaggtcac ccacctgatc gagggcgcct tttcgctgac cgtggacggg
    12181 gaaacccgga ccctcgctcc gggcggggtg gcggtcattc cccccggggt gccgcacagc
    12241 ggccgcgcgg agacggactg cgtcatcatc gacgtatttt atcccccccg cgacgactac
    12301 cgctgaaacg tgcggacggt tttgtccggg agaaccgttt ccccccgtaa ccccttgttt
    12361 ttccggaggc aaaatatctt gaaaaacccc ttcaaaaaag ggccgttttg tgctagggtc
    12421 agtcatccga tggcaaaatt taactacttg aaatcattag agtaaataat ggcaaagatt
    12481 ctctgcatcg ctaaccagaa gggaggggtg ggaaagacca ccacggccat caatctggcc
    12541 gcctcgatcg ccgccgccga gcggcggacg cttctgatcg actgcgacgc ccagggaaac
    12601 gcgagcaccg ggctgggcgt ctcgcgggag gcgatccggg agcaaaacct ctaccaggcc
    12661 ctcatcggca aacgccccct caaggacgtc atcgtcggga ccatgatccc gcagctcgac
    12721 atcgtcccgg ccgaccagga tctaatcggc atcgaggtgg agttcgtccc gctgccggac
    12781 cgggaaaaaa agctgcgtac gctggtccgg gaactcgata cgccctacga gttcatcatc
    12841 ctggactgcc caccgtcgct ggggttcatg actctgaacg ccctggtcgc cgccgacgcg
    12901 ctcatcatcc cgctgcagtg cgaatacttc gccatggagg gtctggggca cctactcaac
    12961 accctgcgcc tggtgaaggc ccacctgaac ccctccctgc agctgggggg gattctcctg
    13021 accatgttcg acacgaggaa cctcctgtca caccgggtca gcgaggatgt tcgacggcat
    13081 tttgggtcga gggtctttca gacctatatt ccccgaaacg tccggctttc ggaaagtccc
    13141 tcccatggac tgcccattct tctgtacgac atccggtccc ggggggccct gtcctatatg
    13201 gacctggcca aagaaattct cgccaacggg aggagataga tgccgccgaa acagtccctg
    13261 ggaaagggtc tgggggcgat gttccccgat cttctgacgg atgcctcgaa tcggcccgcc
    13321 tttatcacct gcgggattga ggcgcttgtg cccaaccgct tccagccccg gcgaaacttt
    13381 cgcgacacgg agcagcatca actggttcag tccatccgac agaacgggat cgttcagccg
    13441 atcctcgtcc ggagggtcga cgaggggtac gagatcatcg ccggcgaacg gcgctggcgc
    13501 gccgcccaga aagccggcct gagcgaggtt ccggtgatcg tccgggaggc gacggaccgg
    13561 gatctggccg aggtgtcgct catcgaaaac ctgcagcggg aggatctgga ccccatcgag
    13621 gaggccaacg cctaccagac cctgatggaa accttcggcc tgtcccagga ggaggtttca
    13681 tcccgggtcg ggcgggatcg ctccacggtg gcgaatacgg tccggctgct caagctgccc
    13741 ggtgaggcca ggcaggcgat catcgatcgg aagatcagcg ccggccatgg ccgcgccctt
    13801 ctggcgctgg acacgttagg ggaacagcgc cgcatcctgg cacaggtcct caaaaactgt
    13861 ctgagcgtcc gggaaacgga agctctggtg cagcgatcca aacgtccgct cccgccggcc
    13921 acggcgaaac gcaaggaccc gtatgttgcg gacctagagc ggaccctgtc gaggaccgtg
    13981 aagtcccctg tgaagatcca tcagggacgg aagaagggtt acatccagat tgcctttcaa
    14041 aataccgagg aattgaaccg tctttcccgg ttgcttctgg gttaagcatt tgtttgaatt
    14101 tatccacaag tgtggatagc cttgttgaca aggatgtggg ttgtggaaat tttgtgggaa
    14161 aaaagcaccg gtatcctcaa ggaaaaaatc agtagccaga atttcgagac ctggatcaaa
    14221 ccggtccgga tttccgccat ggatggtcaa cagattcagc tgaccgttcc caataaattt
    14281 tttcgggact ggctcctcga caattacgtc acgctgatcc gggaagccct gaaggccgcc
    14341 tccggcgtgg atctccagat cgactttcgg atcggccggg acgcgaagtc tcccgccggt
    14401 ggcgcttcag ctccgtccgg tgcacccgcc gcccgttccc gaacggctcg cgtcaaggcg
    14461 ccctcccgca accacccgac cctgaacgcc aattacagct tcgaccgttt cgtggtcggg
    14521 tcctgcaacc agttcgccca cgcggccgcc gtcgccgtcg cggaacagcc agccaaacag
    14581 tataaccccc tgtttatcta cggcggcgtc ggtctgggga aaacgcacct gctcaacgcc
    14641 atcggcctgc agatgcttgc cctgtattcc cagcggaacg tggcctacgt ctccgccgaa
    14701 gaattcatga acgagctgat caactcgatc cgttacgaca agatgcccca gttccgggaa
    14761 aagtaccgac acatcgattg tatgctgatc gacgacatcc agttcatcgc cggcaaggaa
    14821 cggacccagg aggagttttt ccacaccttc aacaccctcc atgacggtgg caaacagatc
    14881 gtcgtgacga gcgacaagtt tccaaaagat atcccgaacc tggaagggcg tttgcgttcc
    14941 cgcttcgaat ggggactgat cgcagatatt cagccaccgg agatggagac caagatcgcc
    15001 atcatcgaaa agaaggccca ggaaaacaac atcaacctcc cgcacaacgc cgccaattac
    15061 atcgcctccc atgccgaatc gaacatccgg gagctcgagg gatttctgat ccggatcggc
    15121 gccttctcct ccctgacggg gcgggagatc gatctcgacc tggtccggga ggtgctgaaa
    15181 aagattctga aacaacgaga aaaggaagaa atttctatag atgatatcgt caaaaccgtc
    15241 gccggaaaac tcaatcttaa aatcgccgac atcaaatctc cgaagaaaaa caagaatatg
    15301 gttctgggac gccaggtggc catgtttctg gcacgtcaga tgacccccgc ctcctacccg
    15361 gacatcggcg ataaaatcgg cggtaaggac cactcgaccg tgatttacgc ctgccagaag
    15421 atctcccggt tgatggaaac ggacaccaaa ctaaaaaaaa cggttcagga catcgaagag
    15481 gttttgcaaa gccgttccta acagaaggcc acagggccat ccacaggacg gaatggtcaa
    15541 gtatagcgat ttaaaaagga aaccggcgat gattgagatg tccacagggc ttattactaa
    15601 tactatttat gtttataatt a
//
LOCUS       DAOOLJ010000039        15522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4038_length_15522_cov_19.332902, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000039 DAOOLJ010000000
VERSION     DAOOLJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4038_length_15522_cov_19.332902"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLW16_07230"
     CDS             <1..540
                     /locus_tag="PLW16_07230"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70734.1"
                     /translation="FFPPLSAPYEFTQSIIHYQQTLYAGVILDVMGSAADTTASEIQN
                     ATSPEEKLRTYIRNVAEAMDRNPWMPPIMLREMAAGGRNFPETVVLGLARIFTILGEI
                     IAEGVRRGVFVPAPPPLIHLMIVGAFAFFKTSAPLRERQAGLGGDWPAGEDRVSGTVA
                     TEVERLVLRALRKTPGEAG"
     gene            648..2018
                     /locus_tag="PLW16_07235"
     CDS             648..2018
                     /locus_tag="PLW16_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB70735.1"
                     /translation="MKGFRTKSGPVPVAVGVALALFLLWPPAAPAQTEDRMTLQESID
                     FALRESLLIHSAREGTKGAEAAQKEAFTGFLPKFSTSYSYTRLNEAPGFTMPGMTLST
                     VPPLSTSPMTVQSGTEDNYNWGVEVRQPVFAGGAILANYEASRLGAEISRIEESLSVQ
                     DTVLEVKAAYLNSLKAEKLLTVANQTHEQLKAHRNVARNFFEVGVIPRNDLLQSEVQL
                     ANGVQAVLRAENALALARTTFNTILRRDIDLPVRLVDILQYRPFTRTFEACLQTALER
                     RGELKQLQLRQDQAREMVRAARSDYFPNVNLVGNYTRFGDEANVSGSDYKDAESWQVM
                     AVANWNFWEWGRTKHRVEANRSRERQAADAVATLRDRITLDVKNAWLQVREAEKQVLV
                     AQQIIAQAEENFRINEERYKEQVATSTDVLDALTLLTRAKSDHANALADYHFYQARLD
                     RAMGVQ"
     gene            2037..3047
                     /locus_tag="PLW16_07240"
     CDS             2037..3047
                     /locus_tag="PLW16_07240"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB70736.1"
                     /translation="MKKRIVIIVFAVLLVGTGLFVYFGQRQNRLAELSYSGTIEATQA
                     NLSFQAAGRVGDVFVKEGERVGKDQRLARLAPEEFNSRVAQAAANLERAVQNRAQIGI
                     VLDLYRRTLPEDIARAEANVRAQRGIAAEADKNYRRYEQLFQRGVVAEKERDALRLNH
                     DTARARLDEGEAALAQARGNLKKIEAARKDLDAAEAFVAASRAALEQVRIQQGYTELR
                     APFAGIITSRNIEPGETVSPGREVLTLTDLAEVDLKIFINETEIGRVRPGQAADVRVD
                     AFPDRVFRGTVTFVSPEGEFTPKIIQTKKERVKLVYLVKVKVPNPDLSLKPGMPADAW
                     LK"
     gene            3044..3799
                     /locus_tag="PLW16_07245"
     CDS             3044..3799
                     /locus_tag="PLW16_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70737.1"
                     /translation="MSEGLIAIDRVSMNFGATEAVRDVTLSVAAGTIFGLVGSDGAGK
                     STLLRMAATMIPPASGRITVGGLDVVRERRRVKPLIGYMPQRFGLYPDLTVEENLEFF
                     MQIFGIPRRERQARKERYLGFSNLLPFLDRQAGNLSGGMKQKLGLACVLVHEPRALIL
                     DEPTNGVDPVSRQEFWEILARMRSNGMTIFVSTAYLDEGERCDRLGLMHRARLLAQGD
                     PETIRGAAPDLEEAVIARIQAVDQELVHDAFQH"
     gene            3780..4634
                     /locus_tag="PLW16_07250"
     CDS             3780..4634
                     /locus_tag="PLW16_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70738.1"
                     /translation="MTPSNTDAIAVRELEKRFGAFVAVDRISFNVKKGEIFGFLGSNG
                     SGKSTTIRMLCGIITPTSGEGRVAGHDILAEPEAVKQSIGYMSQKFSLYEDLTPYENI
                     RFYLGVYNVPERRWRERSDWVLETTRLTDVRDRATRDLPPGWRQRLALGCALLHEPDI
                     LFLDEPTSGVDPVTRRHFWTFIRDLAARGITVFVTTHYMDEATNCERLVLIDAGRIVA
                     QGSPAAIVAAACPDRPGADLNDAFIRLMARGKAPAGRTANLTATPASPDIETPSPFQP
                     GDPGGIDA"
     gene            4631..5770
                     /locus_tag="PLW16_07255"
     CDS             4631..5770
                     /locus_tag="PLW16_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB70739.1"
                     /translation="MNPRKIQAVMRKEVYHLIRDYRSLILAFAIPLLLILLFGYALSL
                     DVDNVETVVVDQDRSALSRDFVRRLDASPYFRVGAYLNDARAIDAALDGDRATLAVVI
                     PPDWTRTIRADRAAPLQVILDGSDPNFANIARGYITAFLERENRRFLMQFLDRRGLGA
                     TALRPPVEARVRVWFNEDLESRNFIIPGIIAVIIMIAGAMLTSLVIAREYENGTMETI
                     KSLPLSAGEFLTGKALPYFAIGLADVLVAVLMGQVLFGIVMKADFWLMILASSLYLAV
                     ALALGLLISIVTKSQLVANQGAVLITFLPSMLLSDFVFPVVNMPRALQVLTYVVPANY
                     YIDILGGIYLKDLGLREMWPAFAVLTGMAAVLAAVNLIRLKREGL"
     gene            5775..6896
                     /locus_tag="PLW16_07260"
     CDS             5775..6896
                     /locus_tag="PLW16_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB70740.1"
                     /translation="MSWLRIRELVRKEFISLFRDKRNRPILIAVPIIQMLVFGYVVNY
                     DIRVIRVALLDQARTAESRMLADTLTGNGIFRIDHTARDARELEALLLKGKVDLAIKV
                     APDFSRKIRRGETAAVQVLADGSMSNMASVRIAYTVTALDILNRRLLRELHPERMDYG
                     RIDARIRTWYNPNLDSQHFFVPGIVAFVIMLISLLLTSIAIIKEKEQGTMEQLVVTPL
                     RPVELIAGKTIPYILISFVQMIVVTALAVFWFRIPLAGSLALLAFATALFLLSSLGVG
                     LFISTVSATQQQAMMTTFFFILPAFMLSGFVFPIANMPEAVQWLTYANPLRFFLVIIR
                     GIFLKGVGMAVLWPQFVALALLGALVFAGAIGRFRKRLD"
     gene            7006..7989
                     /locus_tag="PLW16_07265"
     CDS             7006..7989
                     /locus_tag="PLW16_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70741.1"
                     /translation="MLRIIEDERDIRRCQQQMMRHFKPYLSGRLTVKLGHAGGSFAAR
                     IAWHASPGIWLYSRKLPEGRYWNVFGLGLPEGSSALPITCEINVPVAGVDRKLGGAFA
                     RHRDGRTFVVHRGLIGGAKKGVGKSLFASRFRGVWSALEEGRTVTPVAVIGELSSPRF
                     VRHLSLFVLKIDRLKNLAVASPQLEMPFETVSFREEWIGRAVTETDGAGGLACDHGPI
                     VQDLAAALRARGLRVGNDDRRDLLITDGGGRIGWILQVIPDSSERAVFDGVARLLIYG
                     ADLPNGCRRVLVLPDGADKPHTERLKKLQVDLLPFAWDENRAVFPDLAALL"
     gene            8416..9504
                     /locus_tag="PLW16_07270"
     CDS             8416..9504
                     /locus_tag="PLW16_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB70742.1"
                     /translation="MENPEEVYIELRKHLDQQAVGFPATKSGVEIRILKHFFAPKEAR
                     LALHLNYQPRSALDIHNSIEGADPSLEKLTSMLEEMSRKGAIGTMEKNRTEYFYTMPL
                     LVGMVELKDKFTPQFGVDLAEYMMNGFILSYMSTRVPQMRTIPVEKSIEVEHRITPYD
                     DIRQIINRSEGPIALIKCMCREGALARGKPCRTTSRLETCLNFGDWGRFFIKTGRGRE
                     VTRQEALEILRLNEADGLVLQPSNYQKVDFVCACCGCCCGILNIQKKLPNPAESWSHN
                     YYSTIHAEDCTPCGICAAKCQMKAIAIDPQSGYPAINLDRCIGCGNCVSACPTRAFRL
                     VKVERETVPPEDRTSLYRRLAERAPADG"
     gene            complement(9619..10683)
                     /locus_tag="PLW16_07275"
     CDS             complement(9619..10683)
                     /locus_tag="PLW16_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB70743.1"
                     /translation="MDRDIFRELQQYLDRLSLGFPAAASGVEIQILKRLFSEEDAALF
                     LSMTPRLEAPAEIAARVDRPVDEVAAHLHDMTERGLLFRVQKEGGARYGTIPFAHGIF
                     EFQVGRLDVELARLLDAYFHEAFRTAMVRSASTFLRTVPVQEALDVRQNIAAYEDAVG
                     ILRRTEKIVVAECICRKQRRLVGQGCGKPREVCFMFGSMGQYYLDQGLGRRVDADEAV
                     RILTEAQEAGLVTQPATAQNPGGMCNCCGDCCGVLVGLNALEKPAEMVFSNHAAAFDE
                     TLCNGCEACLDRCQMAAIRWAGDRVAGIDLDRCIGCGLCVTHCPTKALRLVPKAEPRV
                     PPQSTSDQMLAMARHRGLVS"
     gene            complement(10784..11854)
                     /locus_tag="PLW16_07280"
     CDS             complement(10784..11854)
                     /locus_tag="PLW16_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB70744.1"
                     /translation="MSESVHAKLAKRLDKLPIAYPATDSGVEVKILEKWFTEEEAAIA
                     LAMNGFPESVATIAGRLNRDPEALAPILEAMSRKGAIFRIAKGEKRFYNLSPLAEGMW
                     EFHVHTNTLEDIKALRAYFEEEFMVKSWYGTPTSQHRIIPVSESVTDEMEIMTYERAE
                     EIIRAQRKISVAPCICRKEEKMLTGRGCDHPLEVCMAFGVGAYFYIDNGYGREVSTDE
                     ALAILKRAVESGLVLQPGNGQKVWNLCMCCGCCCGLLRALKKFEKPASVAHTNYFATV
                     LEDDCTACGRCEEKCPMEAIRVVDHMATVDRDRCIGCGVCVAACVFDALKLVKKEAAD
                     VYIPPRDVVEMQTRIAKERGLL"
     assembly_gap    11946..12045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12046..12872)
                     /locus_tag="PLW16_07285"
     CDS             complement(<12046..12872)
                     /locus_tag="PLW16_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70745.1"
                     /translation="MIKKLCVIGAGTMGAGIAQVAAESGIDVVLRDVEEAFVERGLGT
                     IRKFIGKKLEKGKISQEAHDQIVGRVKGTTSPEEGFAGVDMVIEAVFESMELKQKIFA
                     EADRLCGPSVILSSNTSTMSISKIASATGRPERVVGTHFFSPVPAMRLVEVIRGEKTD
                     AALIAATVEFCKAIGKTAILCKDVPGFIVNRFLCLLYNEAANQIHQGLATAEDIDLGL
                     KLGSNHPMGPVEIMDMAGVDVVNNALKAMYEMTGEERYKPSPLFEQMIRENRLGRKTG
                     "
     gene            complement(12976..14493)
                     /locus_tag="PLW16_07290"
     CDS             complement(12976..14493)
                     /locus_tag="PLW16_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70746.1"
                     /translation="MEENYDVIVVGAGVGGLTVASLAALEGLKTLLVEQCDRVGGRAL
                     SIRGEEILARGAAWYKGLLAKQYSWIPYSEPDLDEIIAKGMLKGYTLDVGYHGVALNG
                     DGYFLDLDERLGTGVQMVGNLNSTYIGDEWYMDFHAGKIDDRLMKLLQEQKIPMHRFY
                     VAAAKLTEKDYDALETVSVTQWCKDNGLYENKTVFGMIHAVSTLITTINDPDDISIGD
                     IFRYMGTVINPRFKAGRAQWPSGFTTGGIMRWSQAVARRFEELGGTLLLETRVREIAI
                     ENGRATGVVIEDASGASRRIAGGKVVSNIPIQDAFKVADKGAFPGDWADRMEGLYGYG
                     SIAPYFGLNKLVMPEREWKVGVKDTTVIPKEEGFEYDVYMCWNIQSQIDPSCAPGGRH
                     LMTAYAPVTEKEGKDKALMAKACRLIIDYLERRYPGFRKSVDWALFPTAWKLEGVAKS
                     KTQAGTLKAPVEAPGVAGLYFAGDTVKGYGVAMDCAVAAGLICASRITGKDYGVA"
     gene            complement(14689..>15522)
                     /locus_tag="PLW16_07295"
     CDS             complement(14689..>15522)
                     /locus_tag="PLW16_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70747.1"
                     /translation="QKKNFLYRLASGACLGACGITEPGAGSDVAGMKTTARKVDGGYL
                     LNGAKIFITNGGEAQIVTVLAYTDKDKGNRGISMLILEKGDAGFTVGKHEHKMGMHGS
                     DTRELVFEDVFVPADRLLGPEGGGFRTLMTTFNYTRPAVGGQALGIAQGALEASIRYA
                     KERVQFGKTLSSFQGMQWMLAEMALKVETARSIVYRAASMIDSEPDASELPKIASMAK
                     WYASDVAMQVTTDAVQVFGGYGYVREYPMERMMRDAKITQIYEGTNQIQRTIVAGHLL
                     R"
BASE COUNT         2734 a         5230 c         4721 g         2737 t
ORIGIN      
        1 ttttttccac ccctctcagc cccttatgaa tttacacaat cgattataca ctaccagcaa
       61 accctctatg cgggcgtgat cctggacgtc atgggcagcg cggcggacac cacggcaagc
      121 gagatccaga acgcgacgtc accggaggaa aaactcagga cctacatccg gaatgttgcc
      181 gaggcgatgg accggaaccc ctggatgccg cccatcatgc tccgggaaat ggcggcggga
      241 gggcggaatt tcccggagac ggtcgtcctc gggctggcgc ggatcttcac cattttgggg
      301 gagatcatcg cggagggcgt ccggcggggc gtcttcgtcc ccgccccgcc ccccctcatc
      361 catctaatga tcgtgggggc cttcgccttc ttcaagacga gcgccccgct ccgggagcgc
      421 caggcggggc tgggcgggga ctggccggcc ggggaggatc gggtgtccgg gaccgtcgcc
      481 accgaggtgg agcggctcgt cctccgggcc ttgagaaaga cgcccggaga agccggatag
      541 cgggaagagc atgcggggga atcgcgatcc ctctgcataa ccgggaccgc gattccccac
      601 gtccgtcaag attgaatgca tcagtttttc aaagggggag gatagagatg aaaggcttca
      661 ggacaaaatc cggcccggtc ccggttgcgg tgggcgtcgc gctggcgctg tttcttctct
      721 ggccgccggc cgcgccggcg cagacggaag accgaatgac gcttcaggag agcatcgact
      781 tcgcgctccg ggaaagcctc ctgatccatt ccgcccggga gggaacgaag ggggcggagg
      841 ccgcgcaaaa ggaggccttc acggggttcc tgccgaagtt cagcacgtcc tacagctaca
      901 cgcgcctgaa cgaggcgccg ggcttcacga tgcccgggat gaccctgtcg acggtccctc
      961 ccctctcgac ctccccgatg accgtccaat ccggcaccga ggacaactac aactggggcg
     1021 tcgaggtccg gcagcccgtc ttcgccggcg gggcgatcct ggccaactac gaggccagcc
     1081 gcctgggcgc cgagatctcc cggattgagg agtccctgtc ggtccaggac acggtcctgg
     1141 aggtcaaggc ggcctatctg aactccctga aggcggagaa gctcctcacg gtcgccaacc
     1201 agacccacga acagctcaag gcccaccgga acgtggcgcg gaatttcttc gaggtgggcg
     1261 tcatcccccg aaacgacctc ctgcagtcgg aggtgcagct cgccaacggc gtccaggccg
     1321 tgctcagggc ggagaacgcc ctggcgctgg cgcggacgac cttcaacacg atcctgagac
     1381 gggacattga tctgccggtc cgcctggtgg acatcctgca gtaccggccc ttcacgagga
     1441 ccttcgaggc gtgtttgcaa acggccctgg agaggcgggg cgaactgaag cagctccagc
     1501 tccggcagga ccaggcgagg gaaatggtgc gggccgcccg gagcgactac ttcccgaacg
     1561 tcaacctcgt cggcaactac acccgcttcg gcgacgaggc gaacgtttcg ggcagcgact
     1621 acaaggacgc cgaaagctgg caggtcatgg ccgtggccaa ctggaacttc tgggagtggg
     1681 gccgaacgaa acaccgggtg gaggcgaacc ggagccgcga gcgccaggcg gcggacgccg
     1741 tggcgacgct cagggaccgg atcacgctcg acgtgaagaa cgcctggctc caggttcggg
     1801 aagcggaaaa acaggtcctc gtcgcccagc agatcatcgc ccaggcggag gagaacttcc
     1861 ggatcaacga ggagcgctac aaggagcagg tcgccacctc aacggacgtc ctggacgccc
     1921 tgacgctcct gacccgggcc aaatcggacc acgcgaacgc cctggcggac tatcacttct
     1981 accaggcccg gctggaccgc gccatgggcg tgcaataaat cgagggggac gccgccgtga
     2041 aaaaacgcat cgtcatcatc gtctttgccg tgctgctcgt cggcaccgga ctcttcgtct
     2101 atttcggaca gcggcagaac cggctggcgg agctgtccta ctccggcacg atcgaagcga
     2161 ctcaggccaa cctgtccttc caggcggcgg ggcgcgtcgg ggacgtgttc gtgaaggaag
     2221 gcgagcgggt cgggaaggac cagcgcctcg cccggctcgc gccggaggaa ttcaacagcc
     2281 gcgtggccca ggcggcggcc aacctggagc gggccgtcca gaaccgcgcc cagatcggga
     2341 tcgtcctgga cctctaccgg cgcaccctgc ccgaagacat cgcccgggcg gaggcgaacg
     2401 tgcgcgccca gcgggggatc gccgccgagg cggacaagaa ttaccggcgc tacgagcagc
     2461 tcttccagcg gggcgtcgtg gcggagaagg aacgggacgc cttgcgcctg aaccacgaca
     2521 ccgcccgggc gcgcctcgac gagggcgagg ccgcgctggc gcaggcccgc ggcaacctga
     2581 agaagatcga ggcggcccgg aaggacctgg acgccgccga ggccttcgtg gcggcctccc
     2641 gggccgccct cgaacaggtg cgcatccagc aggggtacac cgagctgcgc gcccccttcg
     2701 ccggaatcat cacgagccgc aacatcgagc ccggcgaaac ggtctcgccg ggacgcgagg
     2761 tgctcaccct gacggatctc gccgaggtgg acctcaagat cttcatcaac gaaacggaga
     2821 tcggccgcgt ccgccccggc caggccgccg acgtccgcgt ggacgccttt ccggatcgcg
     2881 tcttccgcgg tacggttacc ttcgtgtctc ccgagggcga attcacgccg aagatcatcc
     2941 agaccaaaaa ggagcgggtg aagctggtct acctcgtcaa ggtgaaggtc ccgaatcccg
     3001 acctcagcct taaacccggc atgccggccg acgcatggct gaaatgagcg aaggattgat
     3061 cgcgatcgac cgcgtctcca tgaacttcgg cgcgacggag gccgtccggg atgtcaccct
     3121 gtccgtggcc gccgggacga tcttcggtct tgtcggctcc gacggcgccg gaaaatcgac
     3181 gctcctgcgg atggccgcga ccatgatccc gccggcgtcg ggacgcatca ccgtgggcgg
     3241 actggacgtc gtccgcgaaa gacggcgggt aaagccgctc atcggctaca tgccccagcg
     3301 cttcggcctg tacccggacc tgaccgtcga ggagaacctg gaatttttca tgcagatttt
     3361 cggcatcccg cgacgcgagc ggcaggcgcg caaggagcgc tacctggggt tctcgaacct
     3421 gctccccttc ctcgaccggc aggcgggcaa cctctccggc ggcatgaagc agaagctggg
     3481 gctggcctgc gtcctcgtcc acgaacccag ggccctgatt ctcgacgagc cgaccaacgg
     3541 cgtcgatccc gtctcgcgtc aggagttctg ggagatcctg gcacggatgc gctcgaacgg
     3601 catgacgatc ttcgtctcca cggcctacct cgacgaaggg gagcggtgcg accgtctcgg
     3661 cctgatgcac cgggcgcgcc tgctcgccca gggcgacccg gagacgatcc ggggcgccgc
     3721 cccggacctg gaggaggccg tcatcgcccg catccaggcc gtggatcagg agcttgtcca
     3781 tgacgccttc caacactgac gccatcgccg tccgggagct ggaaaagcgc ttcggcgcct
     3841 tcgtggccgt cgaccggatc tccttcaacg tgaagaaggg cgagatcttc ggtttcctgg
     3901 ggtcgaacgg atcggggaaa tcgacgacga tccggatgct ctgcgggatc atcacgccca
     3961 cctccggaga gggacgggtg gccgggcacg acatcctcgc cgaaccggaa gcggtcaagc
     4021 agtcgatcgg ctacatgtcc cagaagtttt cgctctacga ggacctgacc ccctacgaga
     4081 atatccggtt ctacctcggc gtctacaatg tccccgagcg ccgctggcgg gagcggagcg
     4141 actgggtcct ggagacgacg cggctcaccg acgtccgcga ccgcgccacg cgggacctcc
     4201 cgcccggctg gcgccagcgc ctggccctgg gctgcgccct tctccacgaa cccgacatcc
     4261 tcttcctcga cgagccgacc tccggcgtcg accccgtgac gcgccgccac ttctggacct
     4321 tcatccgcga cctcgccgcc cggggcataa ccgtcttcgt cacgacccac tacatggacg
     4381 aggccacgaa ctgcgagcgg ctcgtcctga tcgacgccgg ccggatcgtc gcccagggca
     4441 gccccgccgc gatcgtcgcg gcggcctgtc cggaccggcc cggggccgac ctgaacgacg
     4501 ccttcatccg gctgatggcg cggggcaagg cgcccgcggg aaggacggcg aacctgacgg
     4561 caacgcccgc ctccccggac atcgaaaccc cttcgccgtt ccagcccggc gaccccggag
     4621 ggatagacgc atgaacccgc gcaagatcca ggccgtgatg cgcaaggagg tctatcacct
     4681 gatccgggac taccggagcc tgatcctcgc cttcgccatc ccgttgctcc tcattcttct
     4741 cttcggctac gccctgtcgc tcgacgtcga caacgtcgag acggtcgtcg tggaccagga
     4801 ccgctccgcc ctgagccggg acttcgtccg gcgcctcgac gcctcgccct acttccgcgt
     4861 cggcgcttat ctcaacgacg cgcgggcgat cgacgccgcc ctcgacggcg accgggcgac
     4921 cctggccgtc gtcatcccgc cggactggac gcggacgatc cgggcggacc gcgccgcgcc
     4981 gctgcaggtc atcctcgacg gaagcgaccc gaacttcgcc aacatcgccc ggggctacat
     5041 caccgccttc ctcgagcggg agaaccggcg ctttttaatg caattcctcg accgccgggg
     5101 tctcggcgcg acggccctgc gcccccccgt cgaggcccgc gtccgcgttt ggttcaacga
     5161 ggacctcgaa agccggaatt tcatcatccc cggcatcatc gccgtgatca tcatgatcgc
     5221 cggggcgatg ctgacctcgc tcgtcatcgc ccgggagtac gaaaacggga cgatggagac
     5281 gatcaagtcc ctgccgcttt ccgccgggga gttcctgacc ggcaaggccc tcccctactt
     5341 cgcgatcggc ctggccgacg ttctcgttgc cgtgctcatg ggccaggtcc tgttcggcat
     5401 cgtcatgaag gccgatttct ggctcatgat cctggcctcg tccctgtacc tagccgtggc
     5461 cctggcgctg ggcctcctga tctccatcgt gacgaagtcc cagctcgtcg ccaaccaggg
     5521 ggcggtgctc atcaccttcc tgccgtcgat gctgctgtcc gatttcgtct tcccggtcgt
     5581 gaacatgccc cgggcgctcc aggtcctgac gtacgtcgtt cccgcgaact actacatcga
     5641 cattctgggg gggatctacc tgaaggacct gggcctccgc gaaatgtggc cggccttcgc
     5701 cgtcctgacg gggatggccg ccgtgctcgc cgccgtcaac ctgatccgac tgaagcgcga
     5761 ggggctgtga acccatgagc tggctgcgca tccgcgaact ggtccgcaag gaattcatct
     5821 cgctcttccg ggacaagcgg aaccgtccca tcctgatcgc ggtcccgatc atccagatgc
     5881 tcgtcttcgg gtacgtcgtg aactacgaca tccgcgtgat ccgcgtcgcc ctgctcgacc
     5941 aggcccgtac ggccgagagc cggatgctcg cggacaccct gaccggcaac gggatcttcc
     6001 ggatcgacca cacggcgcgc gacgcgcggg agctggaggc cctgctgctg aaggggaagg
     6061 tcgatctcgc gatcaaggtc gccccggact tctcccggaa gatccgccgg ggcgaaacgg
     6121 cggcggtgca ggtcctggcg gacggcagca tgagcaacat ggcctccgtc cggatcgcct
     6181 acacggtgac cgcccttgac atcctgaacc gccgcctcct gcgcgaactg caccccgaac
     6241 ggatggacta cggccggatc gacgcccgga tccggacctg gtacaacccg aacctcgaca
     6301 gccagcactt tttcgttccg ggaatcgtgg ccttcgtcat catgctgatc tcgctgctgt
     6361 tgacctcgat cgccatcatc aaggaaaagg agcaaggcac gatggaacag ctcgtcgtca
     6421 ccccgctcag gcccgtcgaa ctcatcgcgg gcaagacgat cccctacatc ctcatctcct
     6481 tcgtccagat gatcgtcgtg acggctctcg ccgtcttctg gttccgcatc ccgctcgccg
     6541 ggagcctcgc gcttctcgcc ttcgcgacgg ccctgttcct gttgagcagc ctcggcgtcg
     6601 ggcttttcat ctccacggtc tccgcaaccc agcagcaggc gatgatgacg accttttttt
     6661 tcatcctgcc cgccttcatg ctctccggct ttgtcttccc catcgccaat atgccggagg
     6721 ccgtccagtg gttgacgtac gcaaaccccc tgcggttctt cctcgtgatc atccgcggga
     6781 tcttcctgaa gggcgtgggg atggccgtgc tctggccgca gttcgtcgcc ctggcgctac
     6841 tgggcgccct cgtcttcgcg ggggccatcg gccgcttccg caagcggctg gattgagtca
     6901 aagaaaatcc tgttgcggaa tcggagggat ttgtcatagg gtatccttgt catctcatga
     6961 accctccggg gggctcgcaa cgggatccgg acgaggaatg aatccatgct caggatcatc
     7021 gaggacgaac gggacatccg gcgctgccag caacagatga tgcgccattt caaaccttac
     7081 ctgagcggcc ggctgaccgt gaagctgggc catgccgggg gaagcttcgc cgcccgcatc
     7141 gcctggcacg cgtcgcccgg gatctggctt tactcccgga agctccccga ggggcgctac
     7201 tggaacgtct tcggcctcgg tctgccggaa gggtcgtccg cgctgcccat cacctgcgag
     7261 atcaacgtcc ccgtcgcggg cgtcgaccgc aaactgggcg gcgcctttgc ccggcaccgc
     7321 gacggccgga ccttcgtcgt ccaccgcggt ctgatcggcg gcgcgaagaa gggcgtggga
     7381 aagtcgctct tcgcctcccg cttccggggc gtctggtccg ccctcgagga gggtcgcacc
     7441 gtcacgccgg tggccgtgat cggggagctg tcctcccccc gcttcgtccg ccatctgtcc
     7501 ctcttcgtcc tcaagatcga ccgcctgaag aatctggccg tcgcctcccc gcaactggaa
     7561 atgcccttcg agacggtctc cttccgggaa gaatggatcg gccgggccgt aaccgaaacg
     7621 gacggggcgg gcgggcttgc ctgcgaccac ggcccgatcg tccaggacct ggccgccgca
     7681 ctccgcgcac ggggactccg ggtcggcaac gacgaccggc gcgacctctt gatcacggac
     7741 ggcggcggcc ggatcgggtg gatcctccag gtgattcccg atagctccga gcgggccgtc
     7801 ttcgacgggg tcgcgcgcct gctgatctac ggcgcggatc ttccgaatgg ctgccgccgc
     7861 gtcctcgtcc tcccggacgg cgcggacaag ccgcacacgg aacggctgaa gaaactgcag
     7921 gtggacctcc tgcccttcgc ctgggatgaa aaccgggcgg tcttcccgga cctcgccgcg
     7981 ctcctttaga aaacggccct tgacgcgcat gcttctcggc gtggaatcgc aggtcattcg
     8041 gaacacgacg gcctgccaca aacgccttcc tcttctcctt cgttcgaccg cttcgacgag
     8101 gacctgacgg gcgacagcta ccgcggcgaa ggcccctacc ggaaactccg cttcaagttc
     8161 gacgagaaga ccctcggaaa atcgaacccg tccgccgcct tctagcaaac cgatttcccg
     8221 acctgaaaat catgccggaa aaccccggaa gaaaattgct tgaactttgc ggaccggtct
     8281 gtatgattcc gtcgcaagcg actcgacgag gtttgaaaaa gcggctcacc gcgttaaatc
     8341 tcatcgtgaa ggcatggacc cgcggggccc gatccgaaaa acaatgcctg agaaaaaaca
     8401 gggagggtgg ccaacatgga aaatccggag gaagtctaca tcgaactgcg taaacacctg
     8461 gatcaacagg cggtgggatt ccccgccacg aaatccgggg tcgaaatccg gattctcaag
     8521 catttcttcg cccccaaaga ggcgcgcttg gcccttcatc tgaattacca gccccggtcg
     8581 gcgctcgata tccacaacag catcgaaggc gcggatccgt ctttggagaa attgacgtca
     8641 atgctggaag agatgtcgag gaaaggcgcc atcggcacaa tggagaaaaa ccgcaccgaa
     8701 tacttttata cgatgcccct gctggtgggc atggttgaat tgaaggataa attcacgcct
     8761 caattcggcg tggatctggc cgaatatatg atgaacggat tcatcctgtc ctatatgagc
     8821 acccgcgttc cgcaaatgcg gaccatcccc gtggaaaaaa gcatcgaggt cgaacaccgc
     8881 atcaccccgt acgatgacat caggcagatc atcaaccgct cggaaggacc gatcgcgttg
     8941 atcaagtgca tgtgccgcga aggggcgctg gcacgaggga aaccctgtcg gacaacgtcg
     9001 cgtctggaga cctgcctgaa cttcggcgac tggggccggt tcttcatcaa aaccggccgc
     9061 ggaagagagg tcacccgtca ggaggccctg gagatcctgc gcctgaatga agcggacggc
     9121 ctggttcttc agccgtccaa ctatcaaaag gtcgatttcg tctgtgcctg ctgcgggtgt
     9181 tgctgcggaa tcctcaacat acagaaaaaa ctgccgaatc cggccgaatc ctggtcacat
     9241 aactattaca gcacgatcca cgcggaagac tgcaccccct gtgggatctg tgccgccaaa
     9301 tgccagatga aggccattgc catcgatccg cagagcggat atccggcgat caacctcgac
     9361 cgctgcattg gctgcggcaa ctgcgtgtcg gcctgcccga caagggcttt caggctggtg
     9421 aaggtggaaa gggagacggt cccgccggag gatcgcacca gcctgtatcg aaggctggcg
     9481 gagagggcgc ctgcggatgg atgatcgaac gcaacgggcg gcggccggcc gatacccccg
     9541 ggtgatccga cgcccctggg acatttgtca aagaaggccg ggcgccctgt tttccaaggg
     9601 tccggttgac ggcgacgttt acgaaaccag ccctcgatgg cgggccatgg ccagcatctg
     9661 gtcggacgtg ctttgggggg gcactcgcgg ttcggctttc ggaacgagtc gaagggcctt
     9721 cgtcgggcag tgcgtgacgc agagaccaca gccaatgcag cgatcgaggt cgatcccggc
     9781 gacgcggtcg cccgcccacc ggatggccgc catctggcag cggtccagac aggcctcgca
     9841 gccgttgcag agcgtctcgt cgaaagcggc ggcgtggttc gaaaacacca tttcggccgg
     9901 tttctcgagg gcgttcagcc ccacgagaac accgcagcaa tcgccgcagc agttgcacat
     9961 gccgcccgga ttctgggcgg tcgcgggctg cgtcaccagg ccggcctcct gggcctcggt
    10021 caggatccgg acggcctcat cggcgtcgac ccgccgcccc agcccctggt ccaggtagta
    10081 ctggcccatg gacccgaaca tgaagcagac ctcgcggggc ttgccgcagc cctggcccac
    10141 caatctccgc tgctttcgac agatgcactc cgcgacgacg attttctccg tgcgacgaag
    10201 gattccgacg gcgtcctcgt aggcggcgat gttctgccgg acgtccagcg cctcctgaac
    10261 cggcaccgtc ctgaggaagg tcgaggcact cctgaccatg gccgtgcgaa aggcctcatg
    10321 gaagtaggca tcgagcagcc gcgccagctc gacgtccaga cggccgacct ggaactcgaa
    10381 aatgccgtgc gcgaagggaa tcgtcccgta gcgggcgccc ccctcttttt gcacgcggaa
    10441 caagagcccc cgttccgtca tgtcgtgcag atgcgccgcg acctcatcca cgggccgatc
    10501 gacccgggcc gcgatctcgg cgggcgcctc cagccggggc gtcatggata aaaaaagagc
    10561 ggcgtcctcc tcggagaaaa gccgcttcaa gatttggatc tccacccccg atgccgcggc
    10621 cggaaagccg agggacaggc ggtccagata ctgctgaagt tccctgaaaa tatcccgatc
    10681 catcgttccc cccttttttt gaacccgaca cccggcgcgg gcggcacgcg acaggccgcc
    10741 caccgccgcc acggatcaag aatacccctt cccccgttga cccctaaagc agtccgcgct
    10801 ccttcgcgat gcgggtctgc atctcgacca catccctggg cgggatgtag acgtcggccg
    10861 cctccttctt tacgagcttc agggcatcga acacgcaggc ggccacacag acgccgcagc
    10921 cgatacaccg atcccggtcc accgtcgcca tatgatcgac gacccggatc gcctccatgg
    10981 gacacttctc ctcgcagcgg ccgcaggcgg tgcagtcgtc ctccaggacc gtcgcgaagt
    11041 agttcgtgtg ggccaccgac gcgggcttct cgaacttctt gagcgcccgc agcagcccgc
    11101 agcagcaccc gcagcacatg cacaggttcc agaccttctg gccgttgccc ggctgaagga
    11161 cgagccccga ttcgacggcg cgcttgagaa tggccagggc ctcgtccgtc gacacctccc
    11221 gtccgtagcc gttgtcgatg tagaaatagg ccccgacgcc gaaggccatg cagacctcca
    11281 gcgggtgatc gcaaccgcgg ccggtgagca ttttctcttc cttgcggcag atgcacgggg
    11341 cgaccgagat ctttctctgg gcccggatga tctcctccgc gcgctcgtac gtcatgatct
    11401 ccatctcgtc ggtcacgctc tccgagacgg ggatgatccg gtgctggctc gtcggcgtgc
    11461 cgtaccacga cttgaccatg aactcttctt cgaaataagc ccggagggcc ttgatgtctt
    11521 cgagggtgtt ggtatggacg tgaaactccc acatgccctc cgccagtggg gagaggttgt
    11581 agaagcgctt ctcgcccttg gcgatccgga agatggcccc cttgcgcgac atggcttcga
    11641 ggatgggcgc caaggcctcg ggatcccggt tcagacgccc ggcgatcgtt gccacggatt
    11701 cgggaaaccc gttcatggca agggcgattg cggcctcctc ttcggtaaac catttttcca
    11761 ggatcttcac ctccacgccg gagtccgtcg ccggataggc gatcggcagc ttgtccagac
    11821 gcttcgcgag ttttgcatgc acggattcgc tcatggaatc tcctatttgt cgtacgcgta
    11881 gaagccccga cccgtcttgc ggcccaggcg gttttcccgg atcatctgct cgaagagcgg
    11941 cgaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgt cttgcggccc
    12061 aggcggtttt cccggatcat ctgctcgaag agcggcgagg gcttgtagcg ttcctcgccg
    12121 gtcatctcgt acatcgcctt gagcgcgttg ttcaccacgt cgacgcccgc catgtccatg
    12181 atctccaccg gtcccatggg gtggttggag cccagcttca ggccgagatc gatgtcctcg
    12241 gccgtcgcca gcccctggtg gatctggttg gccgcttcgt tgtagagcag gcacaggaag
    12301 cggttcacga tgaacccggg cacgtccttg cacaggatcg cggtcttgcc gatggccttg
    12361 cagaactcga ccgtcgcggc aatcaaggcc gcatcggtct tttccccgcg aatcacctcc
    12421 acgagacgca tggccggaac ggggctgaag aagtgcgtgc cgacgacgcg ctccggccgg
    12481 cccgtcgccg aggcgatctt cgaaatgctc atggtggagg tgttcgacga caggatgacc
    12541 gacggcccgc agaggcggtc cgcctcggcg aagatcttct gcttcagttc catggactcg
    12601 aacaccgcct cgatgaccat gtccacgccg gcgaaccctt cctcggggct cgtggttccc
    12661 ttgacgcgcc cgacgatctg gtcgtgcgcc tcctgggaga tcttgccctt ttccagcttc
    12721 ttgccgatga acttgcggat cgtgcccaag cctcgctcca cgaacgcctc ttcgacgtcc
    12781 cggagaacca cgtcgatacc gctttcggcc gccacctggg caatgcccgc ccccatcgtt
    12841 cccgcgccga tgacgcaaag tttcttgatc atgaatccgt cctttctctg tgatgatctc
    12901 gtcaaacgtc cgttatcgcc ggtcacccgc gagaaaacgg gggaccggtt ctatacgggg
    12961 ttcccggatt cgcgtctacg ccacgccgta atccttcccc gtgatgcgcg acgcgcagat
    13021 gagcccggcc gccacggcac agtccatggc cacgccgtag cccttgaccg tgtcgccggc
    13081 gaagtacagc ccggcgacgc ccggggcctc caccggggcc ttgagcgtcc ccgcctgcgt
    13141 cttcgatttc gccacgccct ccagtttcca ggccgtcggg aacagggccc agtcgacgct
    13201 tttccggaat cccggatagc ggcgctccag ataatcgatg atgagccggc aggccttggc
    13261 catcagcgcc ttgtccttac cttctttctc cgtcaccggg gcataggccg tcatcagatg
    13321 gcggccgccc ggcgcgcagg agggatcgat ctgggactgg atgttccagc acatgtacac
    13381 gtcgtactca aacccctcct ccttcgggat cacggtggtg tcctttacgc cgaccttcca
    13441 ctcccgctcc ggcatgacca gcttgttcag accgaagtac ggggcgatgg aaccgtatcc
    13501 gtaaagccct tccatccggt cggcccagtc gccgggaaag gcgcccttgt ccgcgacctt
    13561 gaaggcgtcc tggatcggga tgttgctgac gaccttcccg cccgcaatcc ttcgggacgc
    13621 gcccgaggcg tcttcgatca ccacgccggt cgcccggccg ttctcgatcg cgatctcccg
    13681 gacgcgggtt tcgagcagga gcgtcccgcc cagttcctcg aaccggcggg ccaccgcctg
    13741 gctccaccgc atgatgccgc cggtggtgaa cccggagggc cactgcgccc gcccggcctt
    13801 gaagcgggga ttaatgacgg ttcccatata gcggaagatg tccccgatgg agatgtcgtc
    13861 gggatcgttg atcgtggtga tcagggtcga gacggcgtgg atcatcccga acaccgtctt
    13921 gttctcgtac aggccgttgt ccttgcacca ctgcgtcacc gagaccgtct ccagggcgtc
    13981 gtagtccttc tccgtgagtt tcgccgcggc gacatagaac cggtgcatcg gaatcttctg
    14041 ctcctggagc agcttcatca ggcgatcgtc gattttgccg gcgtggaaat ccatgtacca
    14101 ttcatcgccg atgtaggtgc tgttgagatt ccccaccatc tggacgcccg ttcccagccg
    14161 ctcgtcgaga tcgaggaagt agccgtcgcc gttgagcgcc accccgtgat agccgacatc
    14221 caatgtatac cccttgagca tccccttcgc aatgatctcg tccaggtcgg gttcgctgta
    14281 ggggatccag gagtactgct tcgccaggag ccccttgtac caggccgcgc cgcgggcgag
    14341 gatctcctcg ccgcggatgc tgagcgcacg gccgccgaca cggtcgcact gctcgacgag
    14401 cagggtcttg agcccttcca gggcagccag gcttgccacg gtcaagccgc cgacgcccgc
    14461 gcccaccacg atcacatcgt agttctcttc catgtccgaa tcctctttcc taacaggctg
    14521 ttgaaaaacg ctcatccgcg gcgttgcgct gcaaatctca tcgctcgacg tatttctata
    14581 tacgtctcgc tcttgaagtt tttgcgcgcc ttgcatctaa acatttttga acagcctgcc
    14641 caatcccggt gtttttctat actctgccag ggtatgtccg tgtccgcgct atcgcagcag
    14701 atgccccgcc acgatggtcc gctggatctg gttcgtgcct tcgtaaatct gggtgatctt
    14761 ggcgtcgcgc atcatccgct ccatcggata ttcccggaca tagccgtagc cgccgaagac
    14821 ctggacggca tcggtcgtaa cctgcatcgc cacatccgag gcgtaccact tcgccatgga
    14881 ggcgatcttc ggcagttccg aggcgtcggg ctcgctgtcg atcatcgagg ccgcccggta
    14941 gacgatcgac cgcgccgtct ccaccttgag cgccatctcc gccagcatcc actgcatccc
    15001 ctggaaggag gacagggtct tgccgaactg gacccgctcc ttggcatagc ggatggaggc
    15061 ctcgagggcc ccttgggcga tgcccagggc ctgtccgccg acggccggcc gcgtgtagtt
    15121 gaaggtcgtc atgagggtcc ggaaaccgcc cccctccgga ccgaggagac ggtccgccgg
    15181 gacgaagacg tcctcgaaca ccagttcccg cgtgtccgat ccgtgcatcc ccatcttgtg
    15241 ctcgtgtttg cccacggtga atcccgcgtc acccttctcc aggatcagca tgctgatgcc
    15301 gcggttgccc ttgtccttgt ccgtgtaggc gagcaccgtc acgatctggg cctcgccgcc
    15361 gttcgtgatg aagatcttgg cgccgttcag gagatacccg ccgtccacct tcctggccgt
    15421 cgtcttcatg ccggccacgt cggaccccgc ccccggctcc gtgatgccgc aggcgccgag
    15481 acaggcgccg gaggccagac ggtagagaaa attcttcttc tg
//
LOCUS       DAOOLJ010000040        15283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4139_length_15283_cov_12.954820, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000040 DAOOLJ010000000
VERSION     DAOOLJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15283
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_15283_cov_12.954820"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PLW16_07300"
     CDS             <1..1156
                     /locus_tag="PLW16_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPB70748.1"
                     /translation="SSSTPWRPMRSVFKSRAKAIIKTGYLTIRGGALKAIAPLIQKQS
                     LPALKSPQNILFIRVDRVGDMVLSTPVFAAAKRAFPEAHLVVLASPVNAPILKNNPHV
                     DEVILYDCSASLGGNLHLLRSLRKRHFDLTFDLHDDDDLKTAWISWMMGSAHRIGYAG
                     HGREIFLHRSLPRGDGKAHFVSRALELLNDVGIDPVHTLPTIYLDDQERSWAKQWIAD
                     HGLREAKLIGIHPGAYYPTQRWPTAYYAELVRLIRERVGAEAILFGGPGDRSVIDEIL
                     SRVPFSVITAIDSSIRRFFAILPHCRVLICNNSGPLHCAVALGVPTISFMGPTVKKRW
                     MPVGNTHQVLRRDDLPCIGCNLGYCKINTHACMETITPEAVLAILKEHFP"
     gene            1168..1899
                     /locus_tag="PLW16_07305"
     CDS             1168..1899
                     /locus_tag="PLW16_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70749.1"
                     /translation="MDLREIRTGNAHRHPWEIARASAILSVIPKGQYDRIVDVGAGDL
                     YFLERFRERYTGDVFAVDPGYMALEYGQGITLLKSLNQIPSSTMDIGFLMDVLEHEEQ
                     DGNLLWEATQKIKPGGLMIITVPAYPAIFSPHDQFLGHIRRYNRSRLRVLTMGIDVTL
                     RECFSFFTLPLCIRILQIALSKVHLYRFKNEGVGSWHLSPSHWLTKLISSVLKTDFVV
                     NRSLNRFGFPTFGLSLCMLLQKKSA"
     gene            2597..3949
                     /locus_tag="PLW16_07310"
     CDS             2597..3949
                     /locus_tag="PLW16_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB70750.1"
                     /translation="MKRFSHLLGKVIHFVDLKKAYGITLFYGIALIVIRLKVGIIWGG
                     DTSQYIEKSVIRPPIYPLLLDLFSYLFGAHEYIALVFFQLIFVLIAAFHLSNTMRKKF
                     HLLPITFTLLSCLLSVPLLSSSVALGLHGEIGNIILTEAISYGLFLFAVAFLVRILFI
                     SRPTDFIIFLSLIVILTLTRTQMIFLYVIAIVLILSLYLKRDIRIPAAISLFIVVLVI
                     FLCAELGERLYHKLTNNYFGKISLTESHLLVGAIYVSDKQTFSHIDDIRDRRVLEQAY
                     EFLEDQKLLAKQRFEIGRRMVDLYNDNFAVILGPGLMSSFRRVYAAPPWGDDMLLQVE
                     SFSRRVAPVLVIHHYKDVAKLMLLKFLYTLNFREGFFFATFLLFPFVKFTRELKLFSF
                     FVLLMLLTNRLMMTPIIYIGDRYLFYTDILEYVALVIMAEEYLRIRSTKGDYSTEIPS
                     "
     gene            4038..5042
                     /locus_tag="PLW16_07315"
     CDS             4038..5042
                     /locus_tag="PLW16_07315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPB70751.1"
                     /translation="MILASKAMRSIRRGYPQSEIHLLTSTEAGSLAQSFPYINRIYFF
                     PIRELRRNKVFILRTVSILFQLRKTIYGIAVNLYRVGSYLGAMKMGGFFSLLKAGKKI
                     GHDRYGFGRFLDVSLPASTFEGRHVADAMQEIAIKAGGIPDDRGIEVYWNPEAETKWR
                     SFFDPIAGKIAIGINPGGDRANRRWAPDRFAAVAARLVERFNARIILLGGPGDRDIAS
                     SIAQAIPSGVSDLSGTIPLDELPYVISRLDLLLTNDSGPMHIAGATKTPLVALFGPED
                     PRLFGPYTTPDLYRIVMKDVPCRPCGDKKCMHPICLDQIHPEEVLAACLEMLQQQSLR
                     "
     gene            5156..7069
                     /gene="asnB"
                     /locus_tag="PLW16_07320"
     CDS             5156..7069
                     /gene="asnB"
                     /locus_tag="PLW16_07320"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPB70752.1"
                     /translation="MCGICGKLDFSGAKVDEVLVRRMTELMEHRGPDDLGVYLNHREK
                     VSCGLGHRRLSIIDLTEAGRQPLCNEDRTLWMVFNGEIYNFVALRKELKSRGHRFSSR
                     TDSEVILHLFEEEGAAAIDRLVGMFAWAIWSETSRTLLLGRDPVGIKPLVYYADAKRF
                     VFASELKALLADPAVPKEIDPEALDLYLSLNYIPAPWTIFRNIRKLRPGHILKVHQGT
                     VTESRYWDIAAASSAPCAPAPSDFEQMTAGLFNALDDAVKSQMVADVPLGAFLSGGID
                     SSVVVGLMARHASKPVKTYTIGYTDMPMFDETAYARQVAAFHRTEHHEIMLDSRAMLG
                     SVPDVLGALDEPFGDSSAVPTYVVSRETARDVSVALSGDGGDELFAGYRMYQGEAWHR
                     RYRRIPRFLREKLIEPVLLALPNARDNPATERFRRMQKFIRGAKDSLPARFMAWNEIF
                     PEATKEALLGEFFRTKTAPAPRLFTSALSALDTDPINRMLYADLSISLPGDMLWKVDM
                     MSMRHALEVRVPLLDPRVVELAFRIGGDWKIRHGRGKYVFIETFKDLLPKSLHRRPKW
                     GFEMPVSRWLKTDLHGLIGTYLAPERIRKQGIFNTSVIGALIGNMMENRTDTSWQIWN
                     LIAFQAWHDSHLR"
     gene            7050..8366
                     /locus_tag="PLW16_07325"
     CDS             7050..8366
                     /locus_tag="PLW16_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB70753.1"
                     /translation="MTPTSDRIKVLHVITRFDKGGSAENTFLTVRHLDRTLYDPVLVI
                     GGGQAGNASDPEADAAHKNRSDAVQSGVRLVALPRLVRNLHPLSDLAAFFGLITIIRR
                     ERPRIVHTHTSKAGILGRWAAWVCRVPVIVHTPHGHVFFGYFGSLTTRLFILLERWTA
                     RITDALIALTPREKAEHIAQCITPVDAFAVIHSGVDLKPFLALAPSALSSATPPDSNP
                     LEDAGVSDGLLRPTETPVAETMRQNLGIPPGATVVATVGRLTAVKDHETLIRAVAECV
                     RQGERLFLVLLGEGDLRADLEALCERLGIAENVRFTGWQPDVAGLMVACDLFCLPSRN
                     EGMGKVLVEAMALGKPIVASDVGGIPDLVRSGENGLLVPVGDVTAWAQAIRELVRNPD
                     LRRRMGETGRRIAPAYSVEAMIKQIDELYAKLLSDRCVQPNPETSP"
     gene            8555..9976
                     /locus_tag="PLW16_07330"
     CDS             8555..9976
                     /locus_tag="PLW16_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70754.1"
                     /translation="MLSLAPFSPAFAQPPAPPEYRPEPALIDLRTTFSDGEYDPDTLV
                     QMAFRKGFQIVIFNDHDRVALEYGLPPFRNCLKYRKELNSINVAGADRYLRTIQGLRK
                     LYPRAVLIPGTESTPFYYWTGSPITGNLTANDPERRILTMGLEKPEDYETLPVLHNTP
                     LRFQLRSALPALAAFGLCFLIGIFFILQKGAWRATGTIIALLGIGLFLNTLFARPSPF
                     DAYHGKQGAAPYQRFIDAVDRKGGLTFWNYPETLSGVRKLGPIQVRTLPYPDMLLETM
                     NYTGFAALYGDNITITEPGNLWDVVLREYCGGFRERPPWGIATADFHKEGGAGQHLGD
                     FQTVLWLREKTTRSVLEALKTGRMYACQGKYPQIPRLDGFSVSAADGGTSAKAISGEE
                     LTLPRNPRIRIRLSGGGAAANQVQVRLIRSGSLIQTFVGTLPLDIDYLDVLETPGERV
                     YYRMDMSGAGKIVSNPIFVKFAP"
     gene            9973..10656
                     /locus_tag="PLW16_07335"
     CDS             9973..10656
                     /locus_tag="PLW16_07335"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPB70755.1"
                     /translation="MIVSVHQPQYLPWLGYFDKIDRADVFVLLDTVQYKKNEWQNRNR
                     IKTAAGPQWLTVPVTYRFPQRIDEVGVNNRDRWQHQQRQALVTNYRKAPFWDGLAPFL
                     DEFLTPTWERLAPLSVFVVRRLVEILGIATPMHLASELGPFPEDPDERLIAIAKHFGA
                     DTYLAGAGGRDYMDLDRYARSGIQVMFQDYRHPVYSQRFGAFEPNLSVLDLLFNHGPE
                     SLNILRGKR"
     gene            10653..11270
                     /locus_tag="PLW16_07340"
     CDS             10653..11270
                     /locus_tag="PLW16_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPB70756.1"
                     /translation="MNILAIGAHPDDIEVGCGGTLAKYARSGHEIYLLVMTEGSMGGK
                     ADVRKKEQARSANLLKPREMIWGGYKDTLLTPHMNRMVHDIETVLKKIAPNFIFVHYE
                     DDTHQDHRALAKATVSATRYIRNVIFYEGPTTQNFAPTVFVDIRETIDEKFAMLLAHR
                     SQVRKTNIEGLSITDVVRSTAVFRGIQGRVPFAEAFVPLRLFINV"
     gene            11430..12506
                     /locus_tag="PLW16_07345"
     CDS             11430..12506
                     /locus_tag="PLW16_07345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB70757.1"
                     /translation="MKKGSVEMHNIPHSRPTVGEEEVQAVAAVLRSGRIAQGEEVRRF
                     EAELARQVDVTDAVAVSSGTAGLHLALVSLEVGAGDEVVIPSFVCAALLNAVRFVGAE
                     PVPADIDPETFNIDARDVERRLTPRTKAIIVPHLFGLPADIRRIAALGVPVVEDCAQS
                     LGSRLAGAPTGSFGVLSVFSFYATKVICTGEGGMVSSRDAGLLARIRDMRDYDEKADD
                     RPRYNYKLTDLQAAMGIAQLRKLSDFIARRRAIASRYDDVINECGIPTPACPPDREAV
                     PYRYVVRTERLEEILAVGREFGIGYRRPVFKPLHRYLGLNGYPHTDSAFASALSIPIY
                     PSLGDADIEAILDSLQSLLSKKQR"
     gene            12512..13666
                     /locus_tag="PLW16_07350"
     CDS             12512..13666
                     /locus_tag="PLW16_07350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPB70758.1"
                     /translation="MVSNKKQTRVSFQRIFWGLSLVVFSCLWVPQVRQLFFAHLGRWL
                     YILLFAASASALLTPIVRLLARRTGIVDRPGGRKIHTQPTALLGGVAVILSFIGALLA
                     NMILDARTVALLTGGVVMAGVGLLDDARSIPARYKLVIQVAVVLFLIYSGIILDLFPI
                     HTTWGYLLNAALTILWIVGLTNAMNFIDGLDGLATGLAAVISLFMGIVAFQTAQPFMG
                     WIAIAMLGSCLGFLPYNFRWNKPASIFLGDAGSTFFGFVLSALAIKAAWADNNPIVSF
                     ATPILIFWVLIFDMTYITLERTITGKVKSFREWIDYVGTDHLHHRLLGIFGDRKKVVL
                     FILFLSATLGISAVTLRDATTRDGVLTVVQALLITVIVSILEYSGRNSSK"
     gene            complement(13811..>15283)
                     /locus_tag="PLW16_07355"
     CDS             complement(13811..>15283)
                     /locus_tag="PLW16_07355"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPB70759.1"
                     /translation="VAIEADAWVALVYEGENKLSGAAAAIDRATGGMIRERLAAGDIK
                     GKLREICVLYPRAGFKCRCILVAGLGKKTEFRTETVRAASAATAQKARALELKTLVFP
                     ADSGTAGLPLPELAEAVVEGAALGLYRFEAYRTQDMEESASPAQVILAVENGRSRPAV
                     ERAVERAQIVSGAVRLVRDLVSTPPGDMTPAALARVATDLASNRRALSVEVLEADRMR
                     EIGMNALLGVGCGSHEAPKFIILTYHGAAADVPPIVLVGKGVTFDSGGLSLKSYENME
                     GMKDDMAGGAVVLATMAAAADLSLPLNLVGLVPAAENLPGGGAYKPGDVLKSLSGRTI
                     EVMNTDAEGRLLLADALNYAERYEPQAVIDVATLTGACVVALGPELIGLFGNDDALKA
                     RLRAAGDATGEAVWELPLWRDYDELIKSDIADFKNTGGREGGAITAALFLSKFVGPHP
                     WAHLDIAGPVWAKKDRPIIPKGASGVGVRLLVRMLRDWPT"
BASE COUNT         2967 a         4778 c         4200 g         3338 t
ORIGIN      
        1 aagctcttca acgccttgga ggcccatgcg gtccgtcttt aaatcccggg caaaagcgat
       61 catcaaaaca ggctatttga caatccgggg aggcgcactg aaggcaatcg cgccgctcat
      121 tcaaaaacag tccctccctg ccctcaagtc tccacaaaat attcttttca tccgcgtgga
      181 cagggtcggg gatatggttc tttccacgcc ggttttcgca gccgcaaaga gggcctttcc
      241 ggaggcgcat ctcgttgttt tagcgtcacc cgtgaatgcc cccattctca agaacaaccc
      301 gcatgtggat gaagtcatcc tttatgactg ctctgcatcc ctgggtggca acctccacct
      361 cttgaggagc ttgcggaaac gccatttcga cttgaccttc gatctccatg atgatgacga
      421 cctaaagacg gcatggattt cctggatgat gggttccgcc catcgtatcg gctatgccgg
      481 ccatggacga gagatttttc tacaccggtc cctgccgaga ggcgatggaa aagctcactt
      541 cgtaagcagg gcgctggaac tcctcaacga tgtcgggatt gaccctgtcc acaccctgcc
      601 gaccatctac ctcgacgacc aggaaagatc atgggcaaaa caatggatcg ccgaccatgg
      661 acttcgggaa gcgaagctca tcgggattca tcccggggcc tattatccga ctcaacgatg
      721 gccgacggca tactatgccg aactcgttcg tttgatccgt gaacgtgtcg gcgctgaagc
      781 gatccttttc ggaggccccg gcgacaggag tgtcatcgac gagatcctct ccagggttcc
      841 cttttccgta ataaccgcaa ttgactcgag tattcgacgg ttcttcgcaa tcttgcccca
      901 ctgtcgggtt cttatctgca acaactccgg cccgcttcat tgcgccgtgg cactgggcgt
      961 gccgaccatc tccttcatgg gcccgaccgt caagaaacgg tggatgcccg tcggcaatac
     1021 gcaccaggtg ttgcgaaggg acgaccttcc ctgcatcggg tgcaatcttg gatattgtaa
     1081 aatcaatacc catgcctgta tggaaaccat cacgccggaa gcggttcttg ccattctgaa
     1141 agaacatttt ccttaataga aatcctcatg gatctgagag aaatcaggac aggaaatgcg
     1201 caccgacatc cctgggagat agcgcgagct tctgctatct tgagcgtaat cccgaagggt
     1261 cagtatgatc ggattgtgga cgtcggcgct ggtgatttgt attttctgga gcgattcaga
     1321 gaacgataca ctggagatgt ctttgccgta gacccaggtt atatggcgct ggaatatgga
     1381 caaggaatta ctctattgaa atcactaaat caaatacctt cctccactat ggatattggt
     1441 tttctgatgg atgttctgga acatgaagaa caggacggga atctcctttg ggaagccacc
     1501 caaaaaatca aacccggcgg cctcatgatc atcacggttc cggcctatcc agcaatattt
     1561 tcccctcacg accaattctt aggacacatc aggcgctata atcgatcgag actccgggtg
     1621 ttgacgatgg gaatcgatgt gacactccgt gagtgttttt cgtttttcac gctgcccctt
     1681 tgtatacgaa tactacagat tgctttgtca aaggttcatc tgtatcgttt caaaaatgag
     1741 ggtgtcggca gttggcacct ttccccatcc cattggttga caaaattaat ctcatccgtt
     1801 ctcaagacag attttgtcgt aaatcgatct ttaaatcgtt tcgggtttcc aaccttcggt
     1861 ctttctttat gcatgctctt gcagaaaaaa tcagcctgat cattccctgc tataacgaag
     1921 cggaacggct aaagttagat gtatttctgg atagttccga attgctcaag ttcttgtttg
     1981 tgaatgatgg ctcaaccgat catacgggag atctaatcag ggcgcacttg aatgagagga
     2041 tgtttctccg ggatcttccc aaaaacgtcg gaaaagccga agccgttcga ttgggtatgt
     2101 tatacgcctt agagacctcc ccattgaacg actcggaatg gatcggttat tgggatgcgg
     2161 atctcgcgac cccgctttct gaaataaaca attttctgac ttacgcatac agttacgaat
     2221 ttcctgtgga atccatttgg gggagtcgcg tctaccgatt gggtagttcc attcgtcgaa
     2281 gctatcggcg acacgtcctg ggacggatgt ttgctacctt cacaggggtt ttccttggaa
     2341 tacaaagtta tgattctcaa tgcggtgcca agctttttag gaagagcctg atccgttgtg
     2401 ctttttcaga tccattcatg tccaaatgga tctttgacgt ggaactgctc atgcggctcc
     2461 aacacaccaa tatcatagaa tatcctctaa ggacttggtc tgatgtctcg ggtagtaaag
     2521 ttcgtattct ccctaacatg tttagaattc tcgctgattt aattcggata aaaacaaaat
     2581 acgggttcat cgaaaaatga aacgattcag tcatcttctc gggaaagtga tccattttgt
     2641 cgatctcaag aaagcttacg ggatcactct tttttacggg atcgctctta tcgttatccg
     2701 gttgaaagtg ggaatcatct gggggggtga tacctctcaa tacatcgaaa agtcagttat
     2761 cagaccaccc atctaccctt tattgcttga tttatttagt tatctgttcg gcgctcacga
     2821 atatattgca ctggtctttt ttcagttgat attcgtttta atagcggcat ttcacctgag
     2881 taatacgatg agaaaaaaat tccatcttct tcccatcaca tttacccttt tgtcgtgtct
     2941 actgtctgtg ccactgttat cttcgagcgt cgctctcgga cttcacgggg aaataggcaa
     3001 cataatccta actgaagcga tcagttatgg gcttttcctt tttgcggtag ccttccttgt
     3061 aagaattctg ttcatcagtc gtccaaccga ttttataatc tttctttcat tgattgtcat
     3121 cttgacactt acgcggacac aaatgatctt tttatacgtg attgccatcg ttttgattct
     3181 ttcgctatat ttaaaacgag atatccgtat ccctgccgct atcagtcttt tcattgtcgt
     3241 tcttgtcatc ttcctctgcg cggagctcgg cgagcgtctg taccataagc tcacaaacaa
     3301 ttacttcggc aaaatctccc tcactgaaag ccatctgttg gtcggagcca tctatgtgtc
     3361 ggacaaacag acattcagcc atattgatga cattcgtgac cgtcgcgtcc ttgagcaagc
     3421 ctatgagttc ttagaagatc aaaagcttct tgcaaagcag aggtttgaaa tcggtcgtcg
     3481 aatggtggat ctttataatg ataatttcgc ggtcatcctg gggcctggcc tcatgtcgtc
     3541 ctttcgacgt gtctatgccg ctccaccatg gggagacgat atgttgttac aagtcgaatc
     3601 cttttcgcgc agggttgctc ccgtcctcgt cattcaccat tataaggatg tcgccaagct
     3661 catgctcctt aaatttctct atacccttaa tttcagggaa ggtttcttct tcgcaacttt
     3721 cctgttgttt ccttttgtga agttcacccg tgaactaaag cttttttcct ttttcgttct
     3781 tctgatgctc ttgacaaacc gtttaatgat gacgcctatc atctacatcg gggatcgcta
     3841 tcttttttat accgacattc ttgaatacgt cgcccttgtc atcatggccg aagaatactt
     3901 gcgaatccgt tcaactaaag gggattactc tactgaaatc ccctcatgaa atacctcagc
     3961 tgggctaaga ccacattgaa gttgacaata tgtcgcaacc acctattctg atcatactcg
     4021 tcgcgggaat tggagatatg atactcgcat caaaggctat gcgctcgatt cgacgaggct
     4081 atccccaatc cgagatacat ctgcttacta gtaccgaggc cggttctctg gcacaaagtt
     4141 ttccctatat aaaccgcatc tacttcttcc cgattcgtga gcttaggaga aacaaggttt
     4201 tcatattgag aactgtctct atactctttc aattaagaaa aacaatctac ggcattgctg
     4261 taaatctcta tcgtgtcggc tcttacctcg gggcaatgaa gatgggaggt tttttttcac
     4321 ttcttaaagc cggcaagaag atcggccacg accgctatgg attcggccgt tttctcgatg
     4381 tttcgctccc cgcctcaacg ttcgagggac ggcatgttgc ggatgcgatg caggagattg
     4441 ccataaaagc cggaggcatt ccagatgatc gtgggatcga ggtctactgg aatccggaag
     4501 ctgaaaccaa gtggcgatca ttttttgatc ctattgcggg caagatcgcg atcggcatca
     4561 atcccggcgg tgatcgggcc aatcgccgct gggcgccgga ccgttttgcc gccgtggccg
     4621 caaggcttgt cgaacgcttc aatgcacgga tcattctatt ggggggaccg ggagatcgcg
     4681 acatcgcctc ttcgattgcg caggccattc cttcgggcgt ctcggatctc tcgggaacga
     4741 tcccattgga tgaactcccc tacgtgatca gtcgcctgga tctccttttg accaacgaca
     4801 gcggtcccat gcacatcgcc ggcgcgacga agactcccct tgtcgccctc tttgggcctg
     4861 aagatcccag gctcttcggc ccttacacca cacccgatct gtatcgcatc gtcatgaagg
     4921 acgtgccctg tcgaccctgt ggggacaaga aatgcatgca ccctatctgc ctggatcaaa
     4981 tccacccgga agaggttctg gccgcctgtc tggagatgct gcaacaacaa tcccttcgat
     5041 agcgcaagaa agcatccgtt ctcccgacat tcacccttcg acaggctcaa ggtgacggcg
     5101 gcgaccgtcg acgagttcat tctacgaaag aggagggtaa acaggcccaa aacacatgtg
     5161 tgggatctgc ggtaaactgg acttcagcgg ggcgaaggtc gacgaggtac tcgtgcggcg
     5221 gatgaccgaa ctgatggaac atcgggggcc cgacgacctc ggcgtctatc tgaatcaccg
     5281 cgagaaggtc agttgcggac tcggccatcg gcggctcagc atcatcgacc tgaccgaggc
     5341 gggacgccag cccctgtgca acgaggaccg caccctctgg atggtcttca acggcgaaat
     5401 ctacaacttc gtcgccctcc gcaaggaact gaagtcccgc ggccaccggt ttagctcccg
     5461 gaccgacagc gaggtgattc tgcacctttt cgaggaggaa ggcgccgcgg ccatcgatcg
     5521 cctcgtcggg atgttcgctt gggcgatctg gtcggagacg tcccgcaccc ttcttctcgg
     5581 acgcgatccc gtcggcatca agccgctcgt ctattatgcc gatgccaagc gtttcgtctt
     5641 cgcctcggag ctcaaggccc tgctcgccga tccggccgtc ccgaaagaga tcgacccgga
     5701 ggcgcttgac ctctatctca gcctgaacta catcccggcg ccatggacga tcttccggaa
     5761 catccgcaag ctccgccccg gccacatcct gaaggttcat cagggcacgg ttacagagtc
     5821 gcgttactgg gacattgcgg cggcatcctc cgccccttgc gcgcccgcac ccagcgattt
     5881 cgagcagatg acggcgggac tattcaatgc gctcgacgac gcggtcaaga gccagatggt
     5941 tgccgacgtt ccgctcgggg ccttccttag cggcggcatc gattcgagcg tggtcgtggg
     6001 gctcatggcc cgccacgcct cgaaacccgt gaagacctac accatcggct acaccgacat
     6061 gccgatgttc gacgagacgg cctacgcccg gcaggtcgcg gctttccatc gaacggagca
     6121 ccatgaaatc atgctcgact cccgggccat gctcgggtcg gtccccgacg tcctgggggc
     6181 cctcgacgaa cccttcggag actcctcggc cgtcccgacc tacgtcgtct cccgcgagac
     6241 cgctcgggac gtgagcgtgg ccctctccgg cgacggcggc gacgagctct tcgccggcta
     6301 ccggatgtat cagggcgagg cctggcaccg ccgctaccgg cgcattccac gcttcctgcg
     6361 cgagaaactt atcgaaccgg tcctcctcgc cttaccgaac gcccgggaca acccggcgac
     6421 ggaacgcttc cggcgcatgc agaaattcat ccggggcgcg aaggatagcc tccccgcgcg
     6481 cttcatggcc tggaacgaga tctttccgga agcgaccaaa gaagcgcttc tgggtgaatt
     6541 ctttcgcacc aagacggctc cggcgccgcg cctctttacc tccgccctct ccgccctcga
     6601 cactgacccg atcaaccgga tgctctacgc tgacctgtcg atctccctgc ccggggatat
     6661 gctctggaag gtggacatga tgagcatgcg ccacgccctg gaggttcgcg ttcccctgct
     6721 cgacccgcgc gtcgtcgaac tggccttccg catcggcggc gactggaaga tccgtcacgg
     6781 ccgtggcaag tacgtcttca tcgaaacctt caaggatctt cttccgaaat ccctgcaccg
     6841 ccgccccaag tggggcttcg agatgccggt gagccgctgg ctgaaaacgg atcttcacgg
     6901 cctgatcggc acctatctcg ccccggagcg gatcaggaag caggggattt tcaatacgtc
     6961 cgttatcggc gccctaatcg ggaacatgat ggaaaacaga accgacacct cctggcagat
     7021 ctggaatctg atcgcctttc aggcctggca tgactcccac ctccgatagg atcaaggtcc
     7081 tccatgtcat cacccgcttc gacaagggcg gctcggcgga gaacaccttc ctcacggtcc
     7141 gccatcttga ccggaccctc tatgaccccg tgctcgtcat cggtggcggg caggccggca
     7201 atgcctccga cccggaggcc gatgccgcgc acaagaaccg ttccgacgcc gttcagagcg
     7261 gcgtccgctt ggtcgccctc ccccgcctgg tccgcaatct ccaccctctt tccgatctgg
     7321 ctgccttctt cggcctgatc acgatcatcc gccgcgagcg tccccggatt gtccacaccc
     7381 acacgtcgaa ggcgggcatc ctcgggcgtt gggcggcctg ggtctgccgc gtgccggtca
     7441 tcgtccatac gccccacgga catgtgtttt tcggatattt cggttctctg acgacgcgcc
     7501 tgttcatcct cctggaacga tggacggcgc ggatcacaga cgccctgatc gccctgacgc
     7561 cgcgcgagaa agcggagcac atcgcgcagt gcattactcc ggtggatgcc ttcgcggtca
     7621 ttcacagcgg cgtcgatctg aagcccttcc tggcgctcgc cccttccgcc ctttcatcgg
     7681 cgacccctcc tgattcgaac cctttggagg acgcgggggt gagtgacggg ttactcaggc
     7741 cgacagaaac gcccgttgcc gagacgatgc gacagaacct gggcattccc ccgggagcca
     7801 ccgttgttgc caccgtgggc cggctgaccg ccgtcaagga ccatgagacc ctcatccgag
     7861 ccgttgccga atgtgtgcgt cagggtgaac gtctgtttct cgtcctgttg ggcgagggcg
     7921 atctgcgggc ggatctcgaa gcgttgtgcg aacgtctggg catagctgaa aacgtacgct
     7981 tcacgggttg gcagccggac gtggcggggc tgatggtcgc ctgcgacctc ttctgcctgc
     8041 cctcgcgcaa cgaaggcatg ggcaaggttc tcgtggaggc gatggcgctc ggcaagccga
     8101 ttgtcgcgag cgacgtgggc ggcattccgg atctcgtccg ttccggcgag aacggccttc
     8161 ttgttcccgt cggcgatgtc acggcctggg ctcaggcgat ccgggaactc gtccgcaatc
     8221 ccgacctgcg acgtcggatg ggcgagaccg gccgccgtat tgcgcccgcc tacagcgtgg
     8281 aagcgatgat aaaacagatt gacgaattgt atgcaaaatt gctaagcgat cggtgcgtcc
     8341 agccgaaccc ggagacctcg ccatgacgtg ccgctcaacc cctcgcccca ccgttcattc
     8401 ccctgcttca cacagacccg tttcgtccgc ctacgagtat tcgcgtggga gtgaactcgc
     8461 acctcgctct acccggagca ccggttatgc cctttgcgcc gagacaaaag cgggcgcttt
     8521 tttcacgccc gccctttttt ttcttatcct cgccgtgctg agcctcgcgc cgttttcccc
     8581 ggccttcgcc cagccgcccg cacccccgga gtaccggccc gagccagccc tgatcgacct
     8641 gcgaaccacc ttcagcgacg gagagtacga cccggacacc cttgtccaga tggccttccg
     8701 caagggcttc cagatcgtca tcttcaacga ccatgaccgg gtagccctgg aatacggcct
     8761 gccccccttc cgcaactgtc tcaaataccg gaaggagttg aactcgatca acgtcgccgg
     8821 ggcggaccgc tacctccgca ccatccaagg cctgaggaag ctttaccctc gggcggtcct
     8881 gattccgggc accgaaagca cccccttcta ctattggacg ggtagtccga tcaccgggaa
     8941 cctgacggca aacgatcccg aacgccgcat cctgacgatg ggcctcgaga agccggagga
     9001 ttacgaaaca ctccccgtgc tccacaatac ccccttgcgc ttccagttgc gctcggcgct
     9061 gccggcgctc gccgcgttcg ggctctgctt tttgatcggc attttcttca tccttcagaa
     9121 aggcgcctgg cgcgcaaccg gtacgatcat cgcccttctc ggcatcggcc tcttcctcaa
     9181 caccctcttc gcccgcccgt ccccgttcga tgcctaccac ggaaagcaag gcgcggcgcc
     9241 ctaccagcgc ttcatcgacg cggtcgatcg aaagggcggg ctcaccttct ggaactaccc
     9301 ggagaccctc tccggcgtgc gcaagctcgg tccgatccag gtccgcacgc tgccctaccc
     9361 cgacatgctc ctggagacga tgaactacac cggatttgcc gcgctctacg gggataacat
     9421 cacgattacg gagcctggca acctctggga cgtcgtgttg cgcgagtact gcggcggatt
     9481 ccgtgagcgc cccccctggg ggatcgcgac cgccgatttt cacaaggaag gcggcgccgg
     9541 tcagcacctg ggcgacttcc agacggtgct ctggctccgt gaaaagacaa cccgatccgt
     9601 cctggaggcg ctcaaaaccg gccggatgta cgcctgccag gggaagtatc cccagatccc
     9661 ccgcctcgac gggttttccg tttccgccgc ggatggaggc acgtcggcca aggcgatctc
     9721 cggggaggag ctgacgctcc cccgcaatcc gcgtatccgc atccggcttt ccgggggcgg
     9781 ggcggcagcg aatcaggtcc aggtgcggct catccgctcc ggttcgctga tacagacctt
     9841 cgtggggacg ctccccctcg atatcgatta tctcgacgtt ctggagacgc cgggcgagcg
     9901 cgtctattac cggatggaca tgagcggggc cggcaagatc gtatccaacc cgatcttcgt
     9961 gaagttcgcg ccatgatcgt gtccgtgcat cagccgcagt acctgccatg gctcggctat
    10021 ttcgacaaga tcgaccgcgc cgacgtcttc gttctgctcg acacggtcca gtacaagaaa
    10081 aacgaatggc agaaccgcaa ccggatcaag acggccgcgg gaccgcagtg gctgacggtg
    10141 ccggtgacct accggttccc gcaacggatc gacgaggtgg gcgtcaacaa ccgggatcgc
    10201 tggcagcatc agcagcgcca ggcgctcgtc accaactacc ggaaggcgcc cttctgggac
    10261 ggccttgccc cgttcctgga tgagttcctg acgccgacct gggagaggct tgcgccgctc
    10321 agtgtcttcg tggtccggcg gctcgtcgag atcctgggca tcgcgacgcc catgcacctc
    10381 gcctcggagc tgggtccgtt cccggaggac ccggatgagc ggctgatcgc gattgcgaag
    10441 cacttcgggg cggacaccta cctggccggc gcagggggtc gggactatat ggatctcgat
    10501 cgatacgcgc ggagcggcat ccaggtgatg tttcaagact accggcatcc ggtctacagc
    10561 caacgcttcg gcgccttcga gccgaacctg tcggtcctgg accttctgtt caatcatggc
    10621 ccggagagtc ttaatattct gagggggaag cgatgaacat cctggcaatc ggcgcacatc
    10681 cggacgacat cgaggtcggc tgcggcggca cattggcgaa atacgcgcgc agcggccacg
    10741 agatctacct gctcgtcatg accgagggca gcatgggcgg caaggcggac gtccgcaaga
    10801 aggagcaggc ccgctcggcg aatctcctca aaccccggga gatgatctgg ggaggttaca
    10861 aggacaccct cctgacgcct cacatgaacc ggatggtcca cgacatcgaa acggtcctca
    10921 agaagatcgc ccccaacttc atcttcgtcc attacgagga cgacacacac caggaccacc
    10981 gcgcgctggc caaggcgacg gtctcggcga cgcgctacat ccggaacgtc atcttctacg
    11041 aagggccgac cacgcagaat tttgccccga ccgtcttcgt ggacatccgc gagacgatcg
    11101 acgagaagtt tgccatgctc ttggcccacc gttcccaggt ccggaagact aacatcgagg
    11161 ggctgtccat caccgacgtc gtgcgctcga ccgcggtttt tcgcgggatc cagggacggg
    11221 ttcccttcgc ggaggccttc gtcccgctgc gtctgttcat caacgtgtag ggatcttttc
    11281 atcatgtcac ttcgagaagc gccagcgacg agaaatcttt cacccccgtg tcatttcgag
    11341 ggagcgccag cgacgagaaa tctttaagat ttctcccttc ggtcgaaagg acaatgggag
    11401 aatcgaaatg acatatatgg gcagtagata tgaaaaaagg gtcggtcgaa atgcataata
    11461 ttccccattc tcgccccacc gtcggggagg aggaggttca ggccgttgcc gcggtgctgc
    11521 ggtccggccg gatcgcccag ggcgaggagg tgcgccggtt cgaggccgaa ctggcccggc
    11581 aggtcgacgt cacggacgcc gtggccgtca gttccggcac cgccggcctg cacctggcgc
    11641 tggtctccct cgaggtcggc gccggggacg aggttgtcat tcccagtttc gtctgtgccg
    11701 cgctcctgaa cgccgtccgc ttcgtcgggg ccgagccggt gccggccgac atcgatccgg
    11761 aaaccttcaa catcgacgcg cgcgatgtcg aacgccgcct gaccccgcgg acgaaggcga
    11821 tcatcgtccc ccacctcttc ggccttccgg ccgacatccg tcggatcgcc gccctcggcg
    11881 tgcccgtcgt cgaggactgc gcccagtccc tggggagccg cctcgccgga gcgccgacgg
    11941 gcagtttcgg ggtcctctcc gtcttttcct tctacgccac aaaggttatc tgtacgggcg
    12001 agggggggat ggtgtcctcg cgcgacgccg gtctgctggc gcgcatccgc gatatgcggg
    12061 attacgacga gaaggcggac gaccgcccac gctacaacta caagctgacg gacctccagg
    12121 cggcgatggg gatcgcgcag ctccggaaac tctctgactt catcgcgcgc cgccgggcca
    12181 tcgccagccg ctatgacgac gtcataaatg aatgcggcat cccgacgccg gcctgccccc
    12241 cggaccggga ggccgtcccg taccgctatg ttgtccggac ggaacgcctg gaagaaatac
    12301 tggccgtggg ccgggaattt ggcatcggct accgccgccc ggtcttcaag ccgctccatc
    12361 gctacctcgg cttgaacggc tatccccata ccgattctgc tttcgcgagt gccctgtcca
    12421 tccccattta cccctcgctc ggcgatgcgg atatcgaggc gatcctggat tcgctgcaat
    12481 ctctcctgtc gaagaaacaa aggtgaacgg cgtggtctcg aataaaaagc agacgcgggt
    12541 atcatttcag cggatcttct gggggctgtc gctcgtcgtc ttctcctgcc tctgggttcc
    12601 gcaggtccgt cagctttttt ttgcccatct cggacgctgg ctctacatcc tgctcttcgc
    12661 cgcctccgcc tccgctctgc tcaccccgat cgttcggctc ctcgcccgtc ggaccggaat
    12721 cgtcgatcgc cccggcggcc gaaagatcca tactcagcct accgccctcc tgggcggcgt
    12781 ggccgtgatc ctctctttta tcggcgcgct tctggcgaac atgatcctcg atgcgcgcac
    12841 ggtcgccctg ctcacgggcg gcgttgtcat ggccggggtc ggcctgctcg acgacgcgcg
    12901 cagcattccc gctcgctaca agctcgtcat ccaggttgcc gtggtccttt ttctgatcta
    12961 ctcggggatc atcctcgacc tgtttcccat ccatacgacg tggggctacc tcctgaatgc
    13021 cgccctcacc atcctctgga tcgtgggcct cacgaacgcg atgaacttca tcgacggcct
    13081 ggacggcctg gccaccggcc tggcggcggt catttccctg ttcatgggca tcgtggcctt
    13141 ccagacggcc caaccgttca tgggatggat cgccatcgcc atgctgggaa gctgcctggg
    13201 attcctcccg tacaactttc gctggaacaa gccggcctcg atcttcctgg gcgacgcggg
    13261 cagcaccttc ttcggcttcg tcctctcggc cctcgccatc aaggccgcct gggcggacaa
    13321 caatcccatc gtttccttcg cgaccccgat cctcatcttc tgggttctga tcttcgacat
    13381 gacctacatc accctggagc gaacgatcac cggcaaggtc aaaagcttcc gggagtggat
    13441 cgactacgtc gggaccgacc acctccatca ccgcttgctg gggatcttcg gggaccggaa
    13501 gaaggtggtg ctcttcatcc tctttctctc cgcgacgctg ggcatcagcg ccgtcaccct
    13561 gcgcgacgcg acaacgcgcg acggcgtcct gaccgtcgtg caggcgcttc tcatcaccgt
    13621 catcgtttcc attctggagt acagcggcag gaattcgtcg aaataggcct tccggcgggc
    13681 ttcgcgacca gcctacaaat tgccgacctt ctcatccgga ttccatcccg ggcgtgaaca
    13741 acaggcctga attttatttc tgatccggct cccgcaagga agggaggggg cggaagcctg
    13801 ctcagggcat ctaggtcggc cagtcgcgca gcatccggac gagcaggcgc acgcccacgc
    13861 ctgaggcccc tttggggatt atgggccgat ccttcttcgc ccagaccgga ccggcgatat
    13921 ccagatgggc ccatgggtgt ggaccgacga acttgctcag gaaaagcgcg gcggtgatgg
    13981 ccccgccctc ccgcccgccg gtgttcttga agtcggcgat gtcgctcttg atcagctcat
    14041 cgtagtcccg ccacagcggc agttcccaca cggcttcgcc ggtcgcgtcg cctgcggccc
    14101 gaagacgggc cttcaaggca tcgtcatttc cgaaaagacc gatgagttcc ggtcccaggg
    14161 ccacgacgca ggcccccgtc agggtggcca cgtcgatcac ggcctgcggt tcataccgct
    14221 ccgcgtagtt cagggcatct gccagcagga ggcggccttc cgcatccgta ttcatgacct
    14281 cgatcgtccg gccggagagc gatttgagca cgtcgcccgg tttgtaggcg cccccaccgg
    14341 ggaggttttc cgcggccggg accagtccga cgaggttgag cggcagggag agatcggccg
    14401 cggccgccat cgtggccagc acgacggccc cgcctgccat gtcgtccttc atcccttcca
    14461 tgttttcata cgatttgagc gaaagccccc cgctgtcgaa ggtcacgccc ttcccgacca
    14521 gaacgatcgg ggggacgtcc gccgcggcgc cgtggtaggt caggatgatg aatttcgggg
    14581 cctcgtggct gccgcacccc acgccgagaa gggcgttcat gccgatctcg cgcatccggt
    14641 cggcctcgag gacctcgacg gagagcgccc gtcgattgga ggcgagatcg gtcgcgaccc
    14701 gcgccagggc ggccggcgtc atgtcgccgg gcggggtcga aaccaggtcc cgcacgagac
    14761 ggacggcgcc ggaaacgatc tgagcccgtt caacggcccg ctcgaccgca gggcgcgaac
    14821 gcccgttctc gacggcgagg atcacctgag ccggggacgc cgattcctcc atatcctggg
    14881 tgcggtaggc ctcaaaacgg tagagtccga gcgcggcccc ctcgacgacg gcctccgcca
    14941 gttccgggag gggcagaccc gcggttcctg aatcggcggg gaaaaccagc gttttcagtt
    15001 cgagcgccct cgccttctgt gccgtcgctg ccgaggccgc gcggacggtc tccgtacgga
    15061 actccgtctt ctttcccagt cccgcgacga ggatgcaccg gcatttaaaa ccggcacggg
    15121 gatacaggac acagatctcc cggagctttc ccttgatgtc gccggcggca aggcgttcgc
    15181 ggatcattcc accggtcgcg cgatcgatcg cggcggcggc tccggagagt ttgttttccc
    15241 cttcatagac tagcgccacc cacgcgtcgg cttcgatcgc cac
//
LOCUS       DAOOLJ010000041        15161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4178_length_15161_cov_14.250033, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000041 DAOOLJ010000000
VERSION     DAOOLJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4178_length_15161_cov_14.250033"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PLW16_07360"
     CDS             complement(<1..1409)
                     /locus_tag="PLW16_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPB70760.1"
                     /translation="MQTGPGSDWERKIVTPEYVLDKIDPGMSIFIGTGVAEPRTLTKH
                     LMSSEMTNLTDLELVQLVSMGDVISFRDESNKDKFRLRTFFSGWLAGEAITSGAVDLI
                     PSRFSDIGRLILKGALRIDVVFVQITPPDSAGYASLGVSVDVAKMAMQRASLIVGEIN
                     ENMPRTLGNTFVNVRDFHYLVRSTEPLIYFPRLPFDDVMDKVAANVASMIEDGSCLSI
                     YSGSFFEALGKHLARKRNLGVHSYFFTDVLMDLVKSGAVNNKKKGYFQDKCLTSYAQG
                     TPELFRWMHENPLIEFQAIDVVSDHLRISLNDRFMAILPARKISLTGSVALHVGKGNV
                     TAGPGQAQDFFSAAEHSRGGKKIIALPSRNLKGDPNIILSVENYPNQFTNSDTLDLIV
                     TDYGVASMIGRTVRERAQALIDIAHPDDRPELIRQAKGANILYPDQIYIAESGHLYPE
                     EVACTHTFRGDLTVRFRAI"
     gene            1599..2900
                     /gene="purB"
                     /locus_tag="PLW16_07365"
     CDS             1599..2900
                     /gene="purB"
                     /locus_tag="PLW16_07365"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPB70761.1"
                     /translation="MIPRYTREKMASIWSPENRYRKWLDVELLACEALAERGEIPPES
                     MNKIRERAAFDVERIHEIEKTTKHDVIAFLTSVTENVGEDGRFIHMGMTSSDVLDTAL
                     ASQMKEAADLLLEDMDALLAVLKEKALAHRRTLMIGRSHGIHAEPITFGLKLALWYAE
                     TCRNRERLVRAREVISAGKLSGAVGTFSFIDPEVEAYVLAKLGLEPESVASQIVQRDR
                     HAEYFAALAITASSIDKFAQEVRLLQRTEVREVEEYFSPGQKGSSAMPHKRNPVLSEN
                     LSGLARLMRSYAIAALEDIPLWHERDISHSSVERVIAPDATILLDFMLGRFTGMIDRL
                     VVYPDRMRENLEMTHGVIFSQKVLLKLIEKGFTREAAYAIVQRNAMESWHQGKPFRIL
                     LERDPEVTGLLTAADLEAAFQIENFLRHVDFIFARVFGEGT"
     gene            2897..3505
                     /locus_tag="PLW16_07370"
     CDS             2897..3505
                     /locus_tag="PLW16_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70762.1"
                     /translation="MNTRKRWRLAAFFVLFVFCGCGEVREYVNMAADRDLSPVYFQAL
                     NAWTREQTVYSQFETRIRVAATLQSPEFRKAYVQEYARNHDWGPESIQMREEAGESTA
                     RDFLEFKLYVYTPEKIDNDFDGPRSIWTVLLVDGQGRKTTPLEIRRIDDLTATTLAFY
                     PYILPYYGIFYSVKFHPPAEGRALTPLRLVLTSVKGRVELAW"
     gene            complement(3568..5175)
                     /gene="nadB"
                     /locus_tag="PLW16_07375"
     CDS             complement(3568..5175)
                     /gene="nadB"
                     /locus_tag="PLW16_07375"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPB70763.1"
                     /translation="MEISTDFLVIGSGIAGLSFAIRAADLGTVSIVTKKEQFESSTNY
                     AQGGIAVVQDAADRFEYHINDTLVCGAGLCHEDVAAFVVREGPERVRELIDWGVSFTR
                     SDNATAAAYDLGREGGHSMRRVLHAKDLTGREIERALMAKAASKRNIRIFENYFAIDL
                     VRKSKLTGVRSPAPDRCLGAYVFDVDRQQVHRFRAKFILLATGGVGKVYLITTNPDIA
                     TGDGVAMAYRAGARIANMEFIQFHPTCLYHPDAKAFLISEAVRGEGGILRLKNGSTFM
                     EHYHPLKSLAPRDVVAKAIDTELKKSGDEYVLLDITHHDCDFLKSRFPNIYETCLQFN
                     IDITRNPIPVVPAAHYLCGGVVVNAYGETSIERLFACGEVSCTGLHGANRLASNSLLE
                     AIVFAHRAVERISSIYRTVEIDPLSIPPWDPGEATESDESVVVSHNWDEIRRCMWNYV
                     GIVRSDKRLERAERRIELISREIDEYYRNFIVTRDLLELRDIATVSSLIVRCARMRKE
                     SRGLHYNLDYPERNDDEWIKDTVIWKP"
     gene            5341..6372
                     /gene="tsaD"
                     /locus_tag="PLW16_07380"
     CDS             5341..6372
                     /gene="tsaD"
                     /locus_tag="PLW16_07380"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB70764.1"
                     /translation="MLVLGIESSCDETAAAVVRDGRDMLSNVIASQIDAHRLYGGVVP
                     EIASRKHIEAILPVIGEALEAGGVTLAEIEGIAVTRGPGLVGSLLVGVSVAKALAFAL
                     RIPLVGVNHLEGHLAAVFLDRPPEFPFVALVVSGGHTHIYLAERFGRFVALGQTRDDA
                     AGEAFDKAAKLLDIGYPGGLVVDRLARRGDRSKIAFPRPMRGSPDFSFSGLKTALLTH
                     LKQRAAPPEGEALADIAASFQEAICDVLVEKTLRAAVDHGVRRVVVCGGVAANSRLRE
                     RFAVDASPHGIEIFIPPPVLCTDNAAMIAVVGDHLLREGRADGYDLNAVSRWPLDAGP
                     EAGCSSRKT"
     gene            6351..6800
                     /locus_tag="PLW16_07385"
     CDS             6351..6800
                     /locus_tag="PLW16_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPB70765.1"
                     /translation="MFIQENLKGWYRRCLSLKGEPRALALGFALGIFIGVTPTIPFHT
                     AIIIGLGILFRQNITAAYLASWLISNPLTIPFFYIAEYELGRYLLGMPGTMPTFNSYT
                     LDAMLLLGWEILLPLLAGGFLLAPLLAVPGFFLARQLFTRIRSDQRP"
     gene            6797..7651
                     /gene="rsmA"
                     /locus_tag="PLW16_07390"
     CDS             6797..7651
                     /gene="rsmA"
                     /locus_tag="PLW16_07390"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPB70766.1"
                     /translation="MRRLRDILREEDIQPRKSLGQCFLGDEGVTRGIVQRARIGVGET
                     VIEIGAGVGLMTEEIARQARRVVAVEIDPVLVRVLERRLGAFRNVEIVAADILRCNLA
                     SLIGTGGGSEKVKIVGNIPYHLSSPILFMMLAQKHLVAEMILMFQKEVADRLLAPPGT
                     KAYGILSVVMDMYAEMQPEIEVPPECFFPPPRIRSTVLTFRMRSRPKCPMRRETIFIP
                     LVKAAFAHRRKTLFNNLRQTGWLDLSEDDLRNRLVVSGIDPRRRAETLTPEEYALLTD
                     VLTTNKYS"
     gene            7837..8697
                     /gene="panC"
                     /locus_tag="PLW16_07395"
     CDS             7837..8697
                     /gene="panC"
                     /locus_tag="PLW16_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPB70767.1"
                     /translation="MQIIHAVKEMRSFADAARSAGKRVAFVPTMGYFHDGHLDLMREA
                     RRRADRVVVSIYVNPTQFGPQEDLDTYPRDFERDRRLAEGVGVDVIFFPDNSEMYPPD
                     YQTHVDVEGVTRNLCGRSRPGHFRGVTTICAKLFNIVKPHVTVFGQKDFQQLTAIKRM
                     VADLNMDLEVVGRPTTREADGLAMSSRNVHLSEVERLSALSLSRSLKMAERRFQEGER
                     DAAKVIRDIRTFVEGHPFASVDYIQICDSRTLRDVPVISERAVLAMAVHVGETRLIDN
                     YMFGETLNLS"
     gene            8730..9101
                     /locus_tag="PLW16_07400"
     CDS             8730..9101
                     /locus_tag="PLW16_07400"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPB70768.1"
                     /translation="MQRFMLKSKLHRATVTDADLHYEGSITIDEGLMEAADFLPFEKV
                     SIYDVSNGERFSTYVIRGKRDSGVICLNGAAARKVSRGDLIIIASYVLVDDADAARWS
                     PRCVLLDEKNRIKKWPRKKLN"
     gene            9136..9543
                     /locus_tag="PLW16_07405"
     CDS             9136..9543
                     /locus_tag="PLW16_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70769.1"
                     /translation="MKKKLNLAFITGLAVIISIGITLYVLAFLIGAMDRFLDWISTPY
                     HPDTLIGFHITEPGLIVIVTFILLSVAATRSYLGKHIVRYVFVPTTPSPTLGYYLMVP
                     TTEVIDVTMTLEEAFTKIIFCGIATPPGNSLFA"
     gene            9626..11050
                     /gene="ahcY"
                     /locus_tag="PLW16_07410"
     CDS             9626..11050
                     /gene="ahcY"
                     /locus_tag="PLW16_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPB70770.1"
                     /translation="MSFLEIDPKQPYRVADLSLAPWGRKEMALAEKEMPGLMAVREKY
                     GKKKPLRGLKITGSLHMTIQTAMLIETLEELGADLRWASCNIFSTQDHAAAAIAQAGS
                     AAVFAWKGESLEEYWWCTEQALTWPDGSGPDLIVDDGGDATLFVHHGVKIEKDPKLLE
                     TAWDNPEMAIVYERLRRGRERNARRWHRATKRIRGVSEETTTGVHRLYQMATAGELLF
                     PAINVNDSVTKSKFDNLYGCRESLLDGIKRATDIMIAGKKVVVCGYGDVGKGCAQSLR
                     AFGARVIVTEVDPICALQAAMEGYEVRRLEEVLESADIIVTATGCCDVVTGRHMEMMK
                     DEAIVCNIGHFDSEIEMRYLERSKVCRRRNIKPQVDQWTLKSGRTIIVLAEGRLVNLG
                     CATGHPSFVMSNSFTNQVLAQIELATKAYRPGVYTLPKKLDEEVARLHLGRLGVSLTK
                     LTSKQAEYLGVDLEGPFKPDHYRY"
     gene            complement(11064..12143)
                     /gene="lptG"
                     /locus_tag="PLW16_07415"
     CDS             complement(11064..12143)
                     /gene="lptG"
                     /locus_tag="PLW16_07415"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HPB70771.1"
                     /translation="MMLLDRYLIREFTRWFGLILSVFVLLYLLVDFFGRLRMFLSNNA
                     LPSQMVAYFLYNLPMIVSLTLPAAILVATLLTLGALSRNSEIIAMKACGVSLYRIFLP
                     IILCAGLLSTFNFLNGEFLVPEANQRAEHIKRVEIQKKEPRGTFKRNELWYRGEKGIY
                     NFKVFDVETLTLHGITINYLDARFHLTHRVDAEWAEWKEGRWQFHNVMIARFADGEFP
                     QLTWAEHQAVDLPESPLDFQVVQKDADQMGYFELGAYVRKLRAEGYDVTRYTADLHGK
                     LAFSFVSLILVLIGVSFSVISERGGGAARSIAAGIVLGFSYWLLHAFALSLGRSGTLP
                     PLLSAWTANLVFFVLAAFLVRRVQT"
     gene            complement(12140..13312)
                     /gene="lptF"
                     /locus_tag="PLW16_07420"
     CDS             complement(12140..13312)
                     /gene="lptF"
                     /locus_tag="PLW16_07420"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HPB70772.1"
                     /translation="MKTLIPRYILREIAAPFVLILFILTFVLLMGKILQIMDMMVNKG
                     IRVFDIAQLVFFLMPSLLAFTLPISLLISILVGLGRLSSDSEILIFKASGLSLYSYLV
                     PVGLLSLIVCILTLITTVFLVPFGNASTKHLLFAIAQQKASIGIQEKVFNDDFSGLLL
                     YADRIPVHGNTMEGVIVYDHRMTGEPSTIVAQRAYLVSDPDTRAVTLRLEDGSTHMVD
                     LRQKSYRLMSFRSYDVNLNISAALQSDRDKKAEKEMTLGELRHQVSGLDMKSKAAREL
                     IIELHQRLTIPVSCLVFGILAIPLGIRAQRTVKSRGFTTGLTVVVLYYLLQLGGEALV
                     ETGYLAPVVGIWSPNVLFGSAALYLFIRTAREEPLLPRQWTDRLSSSINRNRSCTP"
     gene            complement(13437..14153)
                     /locus_tag="PLW16_07425"
     CDS             complement(13437..14153)
                     /locus_tag="PLW16_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB70773.1"
                     /translation="MTVVEWKKEETIAVITMTHGENRHNPVFVEAILKVFDEIEQDPS
                     VTSAVITSDDPKNWSQGIDLTWIMSAYGEKRLQEIKDFMYGLNRMFKRILLIPMPVIA
                     AINGHAFGDGAIMACCCDFRFMRADRGFFCFPEIDISIPFLPGMLAIVKKAFPYYKLE
                     EAAFSGKRYGGAELEMHHVVVKACADSEALLADAISFGKTFNKKRPIYGEMKKRFHKE
                     IIRTIDTEDPAFIEPLALML"
     gene            complement(14275..>15161)
                     /locus_tag="PLW16_07430"
     CDS             complement(14275..>15161)
                     /locus_tag="PLW16_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPB70774.1"
                     /translation="LWTRRQRIDTVIDLELFSRITALLTALSGAARRVGFHRFHTEGL
                     YRGNFLTHRVSYNPHVHIAKNFIALVNAALSPTPEYPYSKTLIKDDELVLAKAAVTDL
                     QQTAVRDRVLERYPRYRSDVHRLVLVNTNASAMLIQRRWPPDHFRRLIQTILNRYDNI
                     LILLTGDPGEAAEAETLKQAVDHPRCVNFAGGIAFHQLPALYAISAFMLTNDSGPPHF
                     AAVTDMPTFVLFGPETPHLYRSLGRTTPIYAGLACSPCVSAANHRKTSCTENRCLQAI
                     TPEQVFEILEPSLETCRE"
BASE COUNT         3120 a         4274 c         4796 g         2971 t
ORIGIN      
        1 ttgatcgccc ggaaccggac ggtcaggtca ccgcggaagg tatgcgtgca ggcgacctcc
       61 tcgggataga ggtgtccaga ttcggcgatg tagatctgat cgggatagag aatgtttgca
      121 cccttggcct gccggatgag ttcgggccgg tcgtcgggat gcgcgatgtc gatcagggcc
      181 tgggcgcgtt cgcgaaccgt ccggccgatc atggacgcca cgccgtagtc ggtgacgatc
      241 aggtcgagcg tatcggaatt ggtgaactgg ttgggataat tttcgacgga caggatgatg
      301 ttcgggtctc ccttgaggtt acggctgggc agggcgatga tcttcttgcc gccgcgggag
      361 tgctccgcgg cggaaaagaa atcctgcgcc tggcccgggc cggccgtaac gttgcccttg
      421 ccgacgtgga gggccacgct gccggtcagg gagatttttc tggccgggag gatggccatg
      481 aaccggtcgt tgagactgat ccgcaggtga tcggagacga cgtcgatggc ctggaattcg
      541 atcaatggat tttcatgcat ccagcggaag agttcgggcg ttccctgggc gtaggacgtc
      601 aggcacttgt cctggaagta gccttttttc ttgttgttga cggcgccgga cttgacgaga
      661 tccatgagaa cgtcggtgaa gaagtaggaa tgaacgccga gattgcgctt gcgcgcgagg
      721 tgcttgccca gcgcctcgaa aaaggaaccg gaatagatgg acaggcaact gccgtcctcg
      781 atcatggagg ctacattggc cgcgaccttg tccatcacgt cgtcgaacgg caagcggggg
      841 aaataaatga gcggttcggt ggaacggacg aggtaatgaa aatcacggac gttgacgaag
      901 gtgttgccaa gggtccgcgg catgttttcg ttgatctctc cgacgatgag gctggcgcgc
      961 tgcatcgcca tcttggccac gtccaccgag acgcccaggc tggcgtagcc ggccgagtcc
     1021 gggggggtga tctggacgaa aacgacgtca atgcgcaggg cgcctttcag gatcagccgc
     1081 ccgatgtccg agaagcggct ggggatgaga tcgacggcgc cggaagtgat cgcctcgccg
     1141 gccagccatc ccgaaaagaa ggtgcgcagg cggaacttgt ccttgttgct ttcgtcccgg
     1201 aaggagatga cgtctcccat gctcacgagt tggaccagtt cgagatcggt caggttggtc
     1261 atttccgagc tcatgaggtg cttggtgagc gtgcgcggtt cggcgacacc ggtgccgatg
     1321 aaaatgctca tcccggggtc gatcttgtcg aggacgtatt cgggcgtgac gatcttgcgt
     1381 tcccaatctg acccgggtcc cgtttgcata gagcgcctcc taagaactgg atagcggggt
     1441 ttcggagggg ttcgaagagg gggtgaaccg cgaccgaagc accctccgga acgatagagg
     1501 gatcggcctc ctttgtcaat ctaaaagacc ttgacgacat tgacgaaatc atattaataa
     1561 ggtcgcgcaa gcccagacgt aatgaaaagg aggggtctat gatcccccgc tatacccgag
     1621 aaaagatggc gtccatctgg agtccggaaa accgataccg gaaatggctg gacgtcgagc
     1681 tcctcgcctg tgaggccttg gctgaacgag gcgagatccc ccccgaatcg atgaacaaga
     1741 tccgggagcg ggccgccttc gatgttgagc gtatccacga gatcgagaag acgaccaaac
     1801 atgacgttat cgcatttcta acctccgtca ccgaaaatgt cggggaagac gggcggttta
     1861 ttcacatggg gatgacgtct tcggacgtcc tcgacaccgc gcttgcctca cagatgaagg
     1921 aagcggccga cctcctcctg gaggatatgg atgccctgct ggccgtcctg aaggaaaagg
     1981 ccctggccca tcgacggacc ctcatgatcg gtcgctccca cggcattcac gccgagccca
     2041 tcaccttcgg gctcaaactg gccctctggt atgccgaaac gtgccgcaac cgggaacgcc
     2101 tcgtacgggc ccgagaggtc atcagcgcgg gaaagctctc cggggccgtc ggcacgttct
     2161 cgttcatcga tcccgaggtg gaagcctacg tgctggccaa gctgggcctt gaacccgaat
     2221 cggtcgcctc gcagatcgta cagcgagacc gtcacgcaga gtactttgcc gcgctggcga
     2281 taacggcttc ctcgatcgac aagttcgccc aggaggtcag gctgcttcag cggaccgagg
     2341 tgcgggaggt ggaggagtac ttctcgcccg gacagaaggg gtcatccgcc atgccgcaca
     2401 agcgcaaccc ggtgctgtcc gagaaccttt ccgggctggc gcgcctgatg cggtcctatg
     2461 cgattgccgc gctcgaggac attcctctct ggcacgaacg cgacatcagc cactcctccg
     2521 tggaacgcgt cattgccccc gacgccacca tcctgctcga tttcatgctc ggccgtttca
     2581 ccggcatgat cgaccggctc gtcgtctatc cggaccgcat gcgtgagaat ctggagatga
     2641 ctcacggtgt catcttctcg cagaaggtcc tcctcaaact catcgagaag gggtttacgc
     2701 gtgaagcggc ctatgccatc gtgcagcgca acgccatgga atcctggcat caagggaaac
     2761 ctttccggat cctgctggaa cgggacccgg aggtgacggg tctgctgacc gccgcggatc
     2821 tcgaagccgc cttccagatt gagaactttc tgcgtcatgt cgatttcatc ttcgcgcggg
     2881 ttttcggaga gggaacatga acacaaggaa acggtggaga ctggcggctt ttttcgttct
     2941 gttcgtgttt tgcggctgcg gggaagtccg ggaatacgtc aacatggccg cagaccggga
     3001 cctgtcgccg gtctatttcc aggcgctgaa cgcctggacg cgcgagcaga cggtttattc
     3061 acagttcgaa acacggattc gcgtcgccgc cacgctgcag agccccgaat tccggaaggc
     3121 gtatgttcag gaatacgcac ggaaccacga ctgggggccg gagtccatcc agatgaggga
     3181 agaggcgggg gaaagcaccg cccgggattt tctggagttc aagctgtacg tttacacgcc
     3241 cgagaagata gacaacgatt ttgacggacc gcgttccatc tggaccgttt tgctcgtcga
     3301 cgggcagggg cggaaaacga ccccccttga gatccggcgc atcgacgatc tcaccgcgac
     3361 aacgctggca ttctatccct acattttgcc ctattacgga atattttaca gcgtcaaatt
     3421 tcatcctccc gccgaaggaa gggcactgac ccccctccgc cttgtcctga ccagcgtgaa
     3481 gggccgagtg gagctcgcct ggtaaagggg cggaccgcaa agcggacggg cctgcgccga
     3541 gcggcgatcc ttcgtcccag gcggccgtca cggcttccag atcacggtgt ccttgatcca
     3601 ctcgtcgtca ttgcgttcgg gataatccag gttatagtga agcccccggc tttcctttcg
     3661 catgcgggcg cagcggacga tcaaagagga gacggtggcg atatcacgca attccagtag
     3721 atcccgggta acgatgaaat tccggtaata ctcgtcaatc tcgcggctga tcagttcgat
     3781 gcgccgttcg gcgcgctcga gccgtttgtc cgagcgaacg atgccgacgt agttccacat
     3841 gcagcggcgg atctcgtccc agttgtggga gacgaccacg gattcatcgc tttccgtcgc
     3901 ctcgccgggg tcccagggag gaatagacag gggatcgatt tccacggtcc ggtaaatgga
     3961 ggagatgcgc tccacggcgc ggtgggcgaa cacgattgcc tcgaggagcg agttgctcgc
     4021 caggcggttg gcgccgtgca ggccggtgca ggagacttca ccgcaggcga acaggcgctc
     4081 aatgctggtt tctccgtagg cgttgacgac cacgcccccg cagaggtagt gcgccgcggg
     4141 tacgaccggg atcggatttc gggtgatgtc gatgttgaat tgcaggcagg tttcgtagat
     4201 attgggaaac cgcgatttca gaaaatcgca atcgtggtgg gtgatgtcaa gcaggacgta
     4261 ctcgtctccg gatttcttca attccgtgtc gatggccttg gcgacgacat cccttggggc
     4321 caggctcttc aacgggtgat agtgctccat gaaggtcgag ccgtttttga gccggagaat
     4381 gccgccctcg ccgcgcaccg cctcgctgat cagaaaggcc ttggcgtcgg ggtgatacag
     4441 gcaggtaggg tggaactgga tgaactccat gttggcgatc cgcgcgcccg cccgataggc
     4501 catggcaaca ccgtccccgg tggcgatgtc cgggttggtc gtgatcaggt aaaccttgcc
     4561 gacccccccg gtggcgagga ggatgaactt cgcccggaag cggtggacct gttgccggtc
     4621 cacatcgaac acataggccc cgaggcagcg atccggcgcc ggcgacctga cgccggtcaa
     4681 tttagatttg cgaaccagat cgatggcgaa atagttttca aagatccgga tgttcctctt
     4741 cgaagcggcc tttgccatca gggctcgctc gatctccctc ccggtcagat ctttggcatg
     4801 gagaacgcgc ctcatggaat ggccgccctc gcgacccaga tcgtaggcgg cggcggtcgc
     4861 gttgtccgag cgtgtaaacg agacccccca atcgatcagt tcccgaacgc gttcgggacc
     4921 ctcccgcacc acgaaggcgg cgacgtcctc gtggcacaga ccggcgccac agaccaaggt
     4981 atcgttgatg tggtattcga aacggtcggc ggcgtcctgg acgacggcga tgcccccctg
     5041 ggcgtagttg gtgctggatt cgaactgttc cttcttggtg acgattgaga ccgtgccgag
     5101 atccgccgcc cggatggcga aactcagccc ggcgataccg ctgccgatca cgagaaagtc
     5161 cgtcgatatt tccatcgtcg ttccgtcgag gtgccgtcgg tcgttttccg gtgacctttc
     5221 cgccggacgt gagcgtcgtg cacgggaagg gcgtagaaaa cggggtttac aaagccccgc
     5281 gattcctata tacgcattca ccggaattgt aaaaagaaaa agccaaaggg gtcgattgtc
     5341 atgctcgttc tgggcataga gtcctcctgt gacgagaccg cggcagccgt cgtgcgcgac
     5401 ggcagagaca tgctctccaa cgtcattgcg tctcagatcg acgcgcaccg cctctacggg
     5461 ggcgtcgtgc cggagatcgc ttcgcgcaag cacatcgagg ccatcctgcc ggtgatcggc
     5521 gaggctctcg aagccggagg ggtgaccctg gccgagatcg aaggcattgc cgtgacccgg
     5581 gggccgggtt tggtgggctc gctgctcgtg ggcgtgtcgg tggcgaaggc cctggcgttt
     5641 gccctccgga ttcccctggt cggggtcaac catctggagg gtcatcttgc cgccgttttc
     5701 ctggatcggc caccggaatt tccctttgtg gcgctggtcg tttccggcgg acacacccat
     5761 atttatcttg ccgagcgctt cggtcgtttc gtcgcgctgg ggcagacgag ggacgacgcg
     5821 gcaggcgaag ccttcgacaa ggcagcgaag ctcctcgata tcggctatcc cggaggactc
     5881 gttgtcgatc ggctggccag gcgtggggac cgcagcaaga tcgccttccc ccgtccgatg
     5941 agaggaagtc ccgacttcag cttcagcggg ctcaagacgg cactgctcac gcatctgaag
     6001 cagcgcgccg ccccccccga aggcgaggcc ctggccgata tcgccgccag ctttcaggag
     6061 gcgatctgcg acgtcctggt ggaaaagacc ctccgggccg cggtggacca tggcgtgcga
     6121 cgcgtcgtgg tctgtggcgg cgttgccgcc aacagccgcc tcagggaaag attcgccgtg
     6181 gacgcgtctc cacacggcat agagatcttc atccccccgc cggtgctctg cacggacaat
     6241 gccgcgatga tcgccgtcgt cggggatcac ttgctccggg aagggcgcgc ggacgggtac
     6301 gatttgaacg ccgtttcacg ctggccgctc gatgcgggtc cggaggccgg atgttcatcc
     6361 aggaaaacct gaagggctgg taccggcggt gtctgagcct gaagggggag ccccgcgccc
     6421 tggccctcgg tttcgccctg gggatcttta tcggcgtcac cccgaccatt cccttccata
     6481 cggccatcat catcggtctg gggatcctgt tcaggcagaa catcaccgcg gcctatctcg
     6541 cttcttggct gatctccaat cccctgacca ttcccttctt ctatatcgcc gaatacgaac
     6601 tcggtcggta tttgctcggc atgccgggaa cgatgcccac cttcaacagc tacaccctgg
     6661 acgccatgct gttgctggga tgggagattc tccttccgtt gcttgcgggc ggttttctcc
     6721 tcgcaccgct cttagccgtt ccgggctttt tcctcgcccg tcagctgttc acccggatcc
     6781 gaagcgacca aaggccatga gacgtctgcg cgacatcctt cgtgaagagg acatccaacc
     6841 gagaaaatcc ctggggcagt gctttctcgg ggacgagggg gtcacccgcg ggattgtcca
     6901 gagggcccgc atcggagtgg gggaaaccgt gatcgaaatc ggggccgggg tggggctgat
     6961 gacggaggag atcgcccgcc aagcccggcg ggtggtggcc gtggagatcg atccggtgct
     7021 cgtgcgggtg ctggaacgtc gactcggggc cttccggaat gtcgaaatcg tcgcggccga
     7081 catcctccgc tgcaacttag caagtctgat cggaaccggc gggggatccg aaaaggtcaa
     7141 gatcgtcggc aacatcccct atcacctgtc cagcccgatc ctgttcatga tgctcgcaca
     7201 gaaacatctg gtcgccgaaa tgatcctgat gtttcagaag gaagtggcgg atcgtctgct
     7261 ggcgcctccg ggcacaaagg cctacgggat tctttccgtc gtgatggaca tgtacgcgga
     7321 aatgcaaccg gagatcgagg tccccccgga atgttttttc ccgcctcctc ggattcgatc
     7381 caccgtgctg acgttccgga tgagaagccg ccccaagtgt ccgatgcgtc gcgaaacgat
     7441 ttttatcccc ttggtcaagg ccgccttcgc ccaccggcgc aagacccttt tcaacaacct
     7501 gaggcagacc ggctggctgg acctgtccga agacgatctc cggaaccgtc tggtcgtgtc
     7561 cggcatcgat ccccgccggc gggccgagac cctaacacct gaagaatatg ccctattgac
     7621 ggatgtcctg acgacgaata aatattcttg acatggtaaa atcattctgg tagggaacga
     7681 aacgccgctt ggatccgcat gtcgcggact gggacggaag ctgggttcct gaccggcagg
     7741 aaaaggttgg gagccgaaaa atcgtgatac ccgatacgat cagcctctcg tcagcgacgg
     7801 agaggctttt ttttggaccc gaggcagcgt acacggatgc agatcatcca cgccgtcaag
     7861 gaaatgcggt ctttcgcgga tgccgctcga agcgcgggca agcgcgttgc ctttgtcccg
     7921 acgatgggat attttcacga cggccacctc gatctgatgc gggaagcccg gcggcgggcg
     7981 gatcgggttg tcgtcagcat ctatgtcaac ccgacgcagt tcggacccca ggaggacctc
     8041 gacacgtatc cccgcgattt cgagagggac cgccgactgg ccgagggcgt gggcgtcgac
     8101 gttatcttct tccccgacaa cagcgagatg tacccccccg attatcaaac ccacgtggac
     8161 gtcgagggtg tgacgcggaa tctctgcggc cgctcgcgcc ccggccattt ccgcggcgtg
     8221 acgaccattt gcgccaagct cttcaacatc gtcaagccgc atgtcacggt cttcggacag
     8281 aaggattttc agcagctcac ggcgatcaaa cgaatggtcg cggatctcaa catggatctt
     8341 gaagtcgtcg gccggccgac cacccgagag gccgacgggc tcgccatgag ttcgcggaat
     8401 gtccatctca gcgaggtaga gaggctatcg gcactttccc tcagccgatc cctgaagatg
     8461 gcggagcgcc ggtttcagga aggcgagagg gatgccgcca aggtgattcg ggacatccgg
     8521 acgtttgtcg aagggcatcc gttcgcatcc gtcgattaca tccagatctg cgacagccgg
     8581 accctgcggg atgtgcccgt catttccgag cgggccgttc tcgccatggc ggtccatgtc
     8641 ggagagacgc ggctgatcga taattatatg ttcggggaga ccctgaacct gtcgtagggg
     8701 ccactataga acgaggggag ggaacagacg tgcaaagatt catgctgaaa tcgaaacttc
     8761 accgggcgac ggtcaccgat gccgatctgc attatgaagg cagcatcacc attgacgaag
     8821 gattgatgga agccgccgac ttccttcctt tcgaaaaggt gtcgatttac gacgtttcca
     8881 acggggagcg cttttccacg tacgtcatcc gggggaaacg ggattccggc gtcatctgtc
     8941 tgaacggcgc cgcggcaagg aaagtctcac ggggcgatct gatcatcatc gccagttacg
     9001 tgctcgtgga cgatgccgat gcggcgcgat ggtcgcctcg ctgtgtgctg ctcgacgaaa
     9061 agaaccgcat caagaaatgg ccgcgcaaga agctgaactg acatccctca aaaagcaaga
     9121 gacgatccag ccggaatgaa gaagaaactc aatctggcct tcatcactgg cctcgccgtg
     9181 atcatatcga tcgggatcac gctgtacgtc ctcgcctttc tgatcggcgc catggatcgc
     9241 tttctcgatt ggatttcgac gccctaccat cccgataccc tgatcggctt tcatatcacg
     9301 gaaccgggcc tgatcgtgat cgtgacgttc atcctcctca gcgtcgccgc gacgcgaagc
     9361 tatcttggga aacacatcgt ccgttacgtc ttcgtcccga ccacaccgag tcccaccttg
     9421 ggatattatc tcatggtgcc gacgacggag gtgatcgacg tgaccatgac cctggaagag
     9481 gcgttcacaa agatcatctt ctgcggaatc gcgacacctc cgggaaactc ccttttcgca
     9541 taaatcggtt gatcgtgaac ggggattgat gttaaaggat cctcatgctg catgcatttt
     9601 atatcacttg aagggagtgt tgcccatgtc attcttggag atcgacccaa agcaaccgta
     9661 tcgcgtcgcc gacctgtcct tggccccctg gggacgaaag gaaatggcgc tggccgagaa
     9721 ggagatgccg ggcctgatgg cggtgcgcga gaaatatggt aagaagaagc ccctcagggg
     9781 gctgaagatt accggcagcc tgcatatgac cattcagacg gcgatgctga ttgaaaccct
     9841 cgaggaactc ggcgctgatc tccgttgggc gtcctgcaat atcttttcca cccaggacca
     9901 cgccgcggcc gccatcgccc aagccgggtc tgcggcagtt tttgcgtgga agggggaaag
     9961 cctggaggag tactggtggt gcaccgagca ggcgttgacc tggccggacg gatccggacc
    10021 ggatctgatc gtggatgatg gcggagatgc cacgctgttc gtccaccacg gggtcaagat
    10081 cgagaaggat ccaaaacttc tggaaacggc atgggacaat ccggagatgg ccattgttta
    10141 cgaacggctc cgccggggcc gggaaaggaa cgcacgacgc tggcaccggg caacgaaacg
    10201 catccgcggc gtctcggagg aaaccacgac cggcgtccat cgtctctatc agatggcgac
    10261 ggcgggggaa ctgctctttc cggccatcaa cgtcaacgac tccgtaacca aatccaagtt
    10321 tgacaatctg tacgggtgcc gcgaatccct gctcgacggc atcaagcggg ccaccgacat
    10381 catgattgcc ggcaagaagg tggtcgtttg cggctatgga gatgtgggga agggctgcgc
    10441 ccaatcgctg cgcgccttcg gcgcccgggt gatcgtcaca gaagtggatc cgatctgcgc
    10501 gctgcaggcg gcgatggagg ggtatgaagt ccgacggctg gaggaggtgc tcgaaagcgc
    10561 cgatatcatc gtcaccgcga cgggatgctg cgatgtcgtg acggggcggc acatggagat
    10621 gatgaaggac gaggccatcg tgtgcaacat cggtcatttc gacagcgaga tcgaaatgcg
    10681 ctatcttgaa cgcagcaagg tttgcaggcg caggaacatc aagcctcagg tcgatcagtg
    10741 gacgctgaaa tcgggccgga ccatcatcgt cctggcggaa ggccgcctgg tgaatctcgg
    10801 ctgtgcgaca ggtcatccca gttttgtcat gagcaacagc ttcacgaatc aggttttggc
    10861 ccagatcgaa ctggcgacaa aggcgtaccg tccgggtgtc tacacccttc ccaagaaact
    10921 ggacgaggag gttgcgcgtc tgcacctggg aaggctgggg gtcagcctga cgaaactgac
    10981 gagtaaacag gcggagtatt tgggcgtcga tctggaggga cccttcaagc cggatcacta
    11041 ccgctattag tccggttggg ccgtcaggtc tgaacgcgcc ggaccagaaa cgcggccaag
    11101 acgaaaaaca cgaggttcgc cgtccacgcg gagaggagcg gcgggagggt acctgatcgt
    11161 cccagggaca gggcgaaggc gtggagcagc cagtacgaaa acccgaggac gatgccggcc
    11221 gcgatgctcc gggccgcccc gccgccgcgc tccgatatca cggaaaagga taccccgatc
    11281 aagaccagaa tcaggctgac gaaggagaat gccagcttcc cgtgtagatc ggcagtgtat
    11341 cgcgtcacgt catagccttc ggcgcgcagc ttgcggacat aggcgccgag ctcgaagtag
    11401 cccatctggt ctgcgtcttt ctgaacgacc tgaaaatcca acggggattc cggcagatcc
    11461 acggcctggt gctcggccca ggtgagttgg ggaaactccc cgtctgcgaa gcgggcaatc
    11521 atcacgttgt gaaactgcca gcggccctcc ttccattccg cccattcagc gtcgacgcga
    11581 tgggtgaggt gaaagcgggc atcgaggtaa ttgatcgtga tgccgtgcag ggtcagggtt
    11641 tcgacatcga agaccttgaa attgtagatc cctttctcgc cgcgatacca taactcattg
    11701 cgcttgaacg tgccccgagg ctcttttttc tggatttcca cgcgcttgat gtgttccgca
    11761 cgctggttgg cctccggaac gagaaactcg ccattcaaga aattgaaggt gctgagcagt
    11821 ccggcgcaga ggatgatcgg aagaaagatg cggtagagac tgaccccgca ggccttcatg
    11881 gcgatgattt cgctgtttcg cgacagggct cccagggtca gcagcgtggc gaccaggatc
    11941 gccgcgggca gggtgagaga aacgatcatc ggcaggttgt aaagaaaata ggcgaccatc
    12001 tgggacggca gggcgttgtt gctcaggaac atccggaggc gtccgaagaa atcgacgagc
    12061 aggtacagca gaacgaagac cgaaaggatc aagccgaacc agcgggtgaa ttcgcggatg
    12121 aggtagcggt cgaggagcat catggggtgc aactccggtt tcggttgatg ctggaagaca
    12181 gcctgtcggt ccattgccgg ggcagcagcg gttcttcccg ggcggtccgg atgaaaagat
    12241 aaagcgccgc cgacccgaag aggacattgg gcgaccagat tcccacaacc ggcgcaaggt
    12301 aaccggtttc cacaagggcc tctccgccta gttgaagtag atagtagagg accacgaccg
    12361 tcagacccgt cgtgaagccc cgggacttga cggtgcgctg ggcccggatg ccgagaggga
    12421 tggccagaat cccgaagacg aggcaggaaa cgggaatggt cagacgctgg tgaagctcga
    12481 tgatcaattc tcttgccgcc ttgcttttca tgtccagccc gctcacctga tgccgaagtt
    12541 ctcccagggt catctccttc tccgccttct tgtcccggtc gctctgaagc gccgccgaga
    12601 tgttcaggtt cacgtcgtag ctcctgaacg acatcaggcg ataggatttc tggcgcaggt
    12661 cgaccatatg cgtgctgccg tcctcgagcc gtaacgtgac cgcccgggta tcgggatcgg
    12721 aaacgagata ggctcgttgc gcgacgatgg tgctgggttc accggtcatg cgatgatcgt
    12781 aaacgatcac cccctccatg gtgttgccgt gaaccgggat gcggtccgcg tagagcaaaa
    12841 ggccgctgaa gtcgtcgttg aagacctttt cctgtatgcc gatactggct ttctgctggg
    12901 cgatggcgaa gagcagatgt ttcgtggagg cgttgccgaa cgggacgaga aaaacggtgg
    12961 tgatgagggt caggatgcag acgatcagcg acaggaggcc caccggaacg aggtacgagt
    13021 aaagactcaa acccgatgcc ttgaagatca ggatttcgct gtccgaggac aaccggccca
    13081 gaccgacgag gatggagatc agcagggaga tgggcagcgt gaacgccagg agggacggca
    13141 taagaaagaa gaccagctgg gcgatgtcga agacgcgaat ccccttgttc accatcatgt
    13201 ccatgatctg gagaattttc cccatgagca ggacaaacgt cagtatgaac agaatcagga
    13261 cgaaaggggc ggcgatttca cgcaagatat agcgagggat gagcgtcttc atcagcgttt
    13321 cttgggggac ttgcgcagag cgtgagcggc atcgaaacgg tgggctgccg gacacgccag
    13381 acggttgcgg gcggcgggga gaaactcaag atcctcccgc gccgcccatt gtccgtttac
    13441 agcatgaggg ccagcggttc gatgaaagcg gggtcttccg tgtcgattgt gcggatgatc
    13501 tctttgtgga agcgcttctt catttcccca tagatcggac gtttcttgtt aaaggtcttg
    13561 ccgaacgaga tggcatcggc gagcaacgcc tccgaatcgg cacaggcctt gacgaccaca
    13621 tgatgcatct cgagttcggc gcctccgtag cgtttgccgc tgaacgccgc ttcctcgagc
    13681 ttgtaatagg ggaaggcctt cttcacgatg gcgagcatgc ccggcagaaa ggggatgctg
    13741 atgtcgatct caggaaaaca gaaaaagccg cgatcggcgc gcatgaagcg gaagtcgcag
    13801 cagcaggcca tgatggcgcc gtcgccgaag gcatggccgt tgatggcggc gatcaccggc
    13861 atgggaataa gcagaattcg cttgaacatc cgattgaggc cgtacatgaa gtccttgatc
    13921 tcctgcagcc gcttttcccc ataagcggac atgatccagg tcaggtcgat tccctgtgac
    13981 cagttcttgg gatcgtccga cgtgatgacc gcggacgtca cggatgggtc ctgttcgatc
    14041 tcatcgaaga ccttgagaat ggcctccacg aagaccgggt tgtgacgatt ttcaccatgg
    14101 gtcatggtga tcacggcgat ggtttcctct tttttccatt ccacgacagt cataaaatcc
    14161 tcctcgtcaa gggtgtaccg gcatcaccgg gtgagggaag aaccgaggca agcctgcagg
    14221 ggagatagca cgaaaacccg tcaaaataca aggaagattg acaaagccgg atcgctattc
    14281 ccggcaggtt tccagggagg gctccaggat ctcgaagacc tgctcgggcg tgatggcctg
    14341 caggcatcgg ttttcagtgc atgaggtctt gcggtgattg gcggcactga cgcagggaga
    14401 gcacgccagc cccgcataga tgggtgtcgt ccgtcccaaa gaacggtaaa gatgcggcgt
    14461 ttcgggtccg aagagaacga aggtcggcat gtcggtgacg gcggcgaagt gcggaggccc
    14521 cgagtcgttc gtgagcatga aggcgctgat ggcatacagg gccgggagtt gatggaaggc
    14581 gatcccgccg gcgaaattga cgcagcgcgg gtggtcgacc gcctgcttga gcgtttcggc
    14641 ctctgcggcc tcaccggggt cgccggtgag aagaatcagg atgttgtcgt agcggttgag
    14701 aatcgtctgt atcaaacggc ggaaatgatc cgggggccag cggcgctgga tcagcatggc
    14761 gctggcgttg gtattgacca gaaccagccg atggacatcg gagcgatagc ggggatatcg
    14821 ttcgaggact cggtcccgaa cggcggtttg ctgaaggtcc gtcacagcgg ccttggccag
    14881 gacgagttcg tcgtctttga taagggtctt ggaatagggg tactccggtg tcggagacag
    14941 cgcggcgttg acgagcgcga tgaagttctt ggcgatgtga acgtgcgggt tgtagctgac
    15001 ccgatgagtc aggaaattgc cgcgatagag cccttcggtg tgaaagcgat ggaatcccac
    15061 gcgccgcgcc gcgccgctca gggcggtcag aagcgccgtg atgcgcgaaa aaagctcgag
    15121 gtcgatgacc gtgtcgatcc gttgacgccg cgtccagagg a
//
LOCUS       DAOOLJ010000042        15117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4192_length_15117_cov_18.500929, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000042 DAOOLJ010000000
VERSION     DAOOLJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 15117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15117
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_15117_cov_18.500929"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1335
                     /gene="ribD"
                     /locus_tag="PLW16_07435"
     CDS             229..1335
                     /gene="ribD"
                     /locus_tag="PLW16_07435"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPB70775.1"
                     /translation="MKADEFYMRRALRLARKGECRVSPNPMVGAVIVKDGRIIGEGFH
                     ACCGENHAEVNAIECAIEDPAGSQFYVTLEPCCHQGRTPPCVERLIALRPARVIVGTI
                     DPNPLVSGRGIAALEAHGIETTVGVLADACAALNERFFKFIQTGRPFVTLKFAQTLDG
                     RIATRTGHSRWISSHPSLRFAHELRSTHDAILVGVGTVINDDPELTVRHVRGRNPLRV
                     ILDSTLRVPPSSRVLSARDDARTLIATVGNADPEKRRRLERAGVEVLTVPPDGAGRVS
                     LPHLLGLLGERRISSVLIEGGAEVITRVLAARLADRLVVVVAPKIIGRGTEAVGDLGH
                     DRMEQALPLTLRRLRRRGRDTVFEFTVEKNPQPV"
     gene            1392..1895
                     /locus_tag="PLW16_07440"
     CDS             1392..1895
                     /locus_tag="PLW16_07440"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPB70776.1"
                     /translation="MPWYALHTRSRHEDRVHLGLSQKGFNAFLPKIEVWSRRKDRRKK
                     IRLPMFPGYLFIELTSLDNQTKLNVLKTFGVVALLGAPHHPDPIPVPDEKIDAIQRIV
                     ASRVEVQHFQYPKVGEPARITDGPFAGIEGVVLKTDFEKEVFVVSIELLRRSVAIRLE
                     GFQIARL"
     gene            2090..2302
                     /locus_tag="PLW16_07445"
     CDS             2090..2302
                     /locus_tag="PLW16_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB70777.1"
                     /translation="MPKGKITIDKELCKECHLCVFACKHSNIIAGKEYNSKGYRPVCF
                     QDDGKCTGCALCAITCPEIAIEVYRE"
     gene            2313..3356
                     /gene="vorB"
                     /locus_tag="PLW16_07450"
     CDS             2313..3356
                     /gene="vorB"
                     /locus_tag="PLW16_07450"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPB70778.1"
                     /translation="MQGNRIIGEAAIRAGCRFYAGYPITPQNEIPEYMAENLRKVEGG
                     VFIQSESEIAAINMVAGAASAGARAMTSSSGPGISLKQEGISSMAAQELPGVIVNMMR
                     GGPGLGNIRPSQGDYFQATRGGGHGDYRTIVLAPGTCQELADLTMDAFDLADRYRIPV
                     VLLADGMIGQMMEPVVFKEPKPRQYSADYVLKGAGTGKAKTIKGLILDPLDMEEHNWK
                     LDRKYRVIAAKETRCEIFNTDEADIVVVAYGTAARVAKGAIKRLGDHGIKAGLFRPIS
                     LWPFPTEELRRLAGTRKEFFVFEMSTGQMLEDVRLALQGYADIAFHGRPGGVVPTPVE
                     LANVIVRQYRRDR"
     gene            3383..4162
                     /locus_tag="PLW16_07455"
     CDS             3383..4162
                     /locus_tag="PLW16_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB70779.1"
                     /translation="MKKVFSRPESLRKKFVFHYCPGCGHSIAHRVIAEIIDELGVRGT
                     TIGVPPAGCAVLAYNYFDVDMIEAMHGRGPAVATGIKRVRPECLVFSYQGDGDLAAIG
                     IAETFHAANRGENFTIIFMNNAVYGMTGGQMAPTTLLDQKSTTSPPGRNAVLDGYPVR
                     ISEILAMLPGTAYIERCTVNSPANIVKTKRAIKKAFQYQLDGRGFSLIEILSPCPTNW
                     KMSPIDACRWIDDVMSKQFPIGVIKDEPAKAAGKEGAGHDR"
     gene            4152..4691
                     /locus_tag="PLW16_07460"
     CDS             4152..4691
                     /locus_tag="PLW16_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB70780.1"
                     /translation="MIVKTIFSGFGGQGVLMMGYSFAHGAMSEGYEVTYLPAYGAEVR
                     GGTANCTVAVGDEEIASPIASEPDYLVVLNTPSLYTFQNRIAGGGLLFLNSSIISARP
                     SRQDMEVFAVPASDIAEELGNARAFNTVMMGAFLKRTSLVKPEIYLKSLETVMGSKKK
                     AVVEINRKAFSAGYDFLKA"
     gene            4735..5163
                     /locus_tag="PLW16_07465"
     CDS             4735..5163
                     /locus_tag="PLW16_07465"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70781.1"
                     /translation="MPVKQISVLLDNTPGTLARLSNILDNEDITYKAVLAASAEQQST
                     VRLVVNDPERAAAVLKSFGFNFEVSPVLAAEVPLHPGGMNAILKHLSAADVNIHYLYT
                     TINRLGQETIVILGVDNLDEARKVLQRNWIRLIEDEIYSL"
     gene            5220..5630
                     /locus_tag="PLW16_07470"
     CDS             5220..5630
                     /locus_tag="PLW16_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPB70782.1"
                     /translation="MAISPEKEQALRDRMARLGVTEDELAESFIRSSGPGGQNVNKTS
                     TCVYLVHKPTGLSVKCQQERSQAQNRFLARRLLLDRIERLQTGRVEAARQRAEKIRRQ
                     KRRRSRRAQEKILEAKHRRSEKKALRAKPDPSTP"
     gene            complement(5741..6115)
                     /locus_tag="PLW16_07475"
     CDS             complement(5741..6115)
                     /locus_tag="PLW16_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPB70783.1"
                     /translation="MKRVTVGTFVGMLFVLLTATLAAAAQGAAAAAPVVDYTKAIVIG
                     CSLLAAGLAIGMGTIGTGVGMGNGLSGATSAVGRNPEAQGKVLLTMMVGLAMIESLAI
                     YALVIALVVLYANPLLKVVVGG"
     gene            6482..6952
                     /locus_tag="PLW16_07480"
     CDS             6482..6952
                     /locus_tag="PLW16_07480"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70784.1"
                     /translation="MKTDRAAGVAAILQKNIPLVKRPFRAIGERLGLAEADVLRIVRE
                     LKREGVIRKVGAILRHQPAGYVHNALILWAVPDGRTDEVGRILAGFPDVTHCYERRPP
                     FAGKYTLFSMVHMRENRQAELLDTLTQATGITDIQILASVEEFKKSSMEYFDEP"
     gene            6942..>8062
                     /gene="hemL"
                     /locus_tag="PLW16_07485"
     CDS             6942..>8062
                     /gene="hemL"
                     /locus_tag="PLW16_07485"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPB70785.1"
                     /translation="MSPESSEALFDRARQCIPGGVNSPVRACGAVGAIPRFIERAAGA
                     HLYDVDGNAYVDYVGSWGPMILGHAHPRVTAAIQAAAERGTSYGAPTAAEVEMAELIR
                     AAFPAIEQVRMVSSGTEATMSAIRLARGVTGRERIVKFSGGYHGHGDSLLVRAGSGVA
                     TLGIPGSPGIPAALAALTLVLPYNDAGAAEAAFARCGGEIAAVIVEPVAGNMGVVNPA
                     PGFLETLRALTRASGALLIFDEVITGFRLTWGGYQALCGIAPDLTCLGKIIGGGLPVG
                     AFGGRADLMAHLAPAGDVYQAGTLSGNPLAMAAGLATLRILRERQDDYAVLDRRTALL
                     CDEILTLFRQRGRPARINRAGSLFTLFFTDGDVTDYETAL"
     assembly_gap    8063..8162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8163..>9260
                     /locus_tag="PLW16_07490"
     CDS             <8163..>9260
                     /locus_tag="PLW16_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPB70786.1"
                     /translation="VQMARHFDVVGFDIRPERIAELEAGRDRTLEVAPDALKAVAIAF
                     SSDPAVLKTCRLIIVAVPTPIDEARRPDLRPVEQACVLVGRNLARGTCVVFESTVYPG
                     VTEEVCVPLLSRESGLSLGEGFTVGYSPERINPGDRVHTLESITKIVSGSDAATLKLL
                     VDVYGRVVRAGIHPAASIKIAEAAKVIENTQRDINIALMNELAIIFQRMGIDTLDVLA
                     AAGTKWNFLPFRPGLVGGHCIGVDPYYLTYKAEMLGYHPEMILAGRRINDNMGKYVAE
                     RTIKLLIGAGTQIREATIAVLGLTFKEDVPDLRNTRVIDIIRELQDYGIRTVVHDPLA
                     DPDEVERYFGLTLHDLKDIGPVDAVIVAVAHA"
     assembly_gap    9261..9360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9361..10008
                     /locus_tag="PLW16_07495"
     CDS             <9361..10008
                     /locus_tag="PLW16_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB70787.1"
                     /translation="HLVFASSSSVYGGNTRMPFSVHDNVDHPVSLYAATKKSNELMAH
                     AYAALYGLPCTGLRFFTVYGPWGRPDMALFLFTRAILAGEPIDVYNHGRMQRDFTYID
                     DIVEGVSRILDLIPAPDPRWRGEAPDPATSFAPYRIYNIGNNAPVALTDFIAAIEAAL
                     GRKAVRNPLPLQPGDVPATYADVDDLTAAVGFKPSTPLAEGIGRFIRWYRDYYGA"
     gene            10161..11192
                     /gene="gmd"
                     /locus_tag="PLW16_07500"
     CDS             10161..11192
                     /gene="gmd"
                     /locus_tag="PLW16_07500"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB70788.1"
                     /translation="MKKAFITGITGQDGSYLAEFLLGKGYEIHGLIRRSSTFNTDRID
                     HLYRDFHDPEARVYLHYGDLSVSGQLMDLLGDIRPDEIYHLGAQSHVRVSFDMPEYTG
                     DVTGLGTLRLLEAIRKTGIPARFYQASSSEMFGAAPPPQSEATPFHPQSPYAAAKVYA
                     YYIVRNYREAYGLFATNGILFNHESPRRGETFVTRKITRAATRIKLGLQDKLYLGNLQ
                     AKRDWGFAGDYVEAMWLMLQQETPDDFAIATGEMHSVQEFVDRVFAKLDLDPGRHVAI
                     DPRYFRPTEVDALKGDASRARTILGWTPRVDFDGLVEMMVAADMELAMREKTLVEAGY
                     ACNGSSRMI"
     gene            11220..12158
                     /locus_tag="PLW16_07505"
     CDS             11220..12158
                     /locus_tag="PLW16_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPB70789.1"
                     /translation="MTDMRNRRITVTGGKGFLGAHLLRELDAKGCRRVSVADLPEYNL
                     VRIEDVRRLYEEQRPDIVIHLAAKVGGIGFNEANPAPLFYDNLMMGVQLMHEGSLRKI
                     SKFVALGTICAYPKFTPVPFREDDLWNGYPEETNAPYGLAKKMMLVQAQAYRRQYGFN
                     AIFLLPVNLYGPGDNFDPASSHVIPALIRKCVDAKRAGAPEIVVWGTGAATREFFYVE
                     DAAEAIALATEKYDGPEPVNIGAGFEISIRDLTELIVELTRFTGRVVWDATRPDGQPR
                     RMLNTDRARDAFGFQARTAFREGLAKTIAWYERTRP"
     gene            12290..12574
                     /locus_tag="PLW16_07510"
     CDS             12290..12574
                     /locus_tag="PLW16_07510"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPB70790.1"
                     /translation="MDRATRKSAIQLAYASSIGIAMVMMVFGGLFFGLYLDRKLDTGN
                     TFTFVFLVIGLVAGFRNIYVLIRKNFTDERDVITCVKSEPHRKRPTPKKT"
     gene            12534..12929
                     /locus_tag="PLW16_07515"
     CDS             12534..12929
                     /locus_tag="PLW16_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPB70791.1"
                     /translation="MNPIVKDPLQKRLEVTNWVLLALLTLLSALWASPRFVAGIVCGG
                     LICIINFHWLYRNLTTVFAQLTEKAKSAVMIRYVLRFVLTAAVLFVVITREWVDIFGL
                     VIGLSIVVVNVVLTIVLVYGKKNLIQEVR"
     gene            12933..13586
                     /gene="atpB"
                     /locus_tag="PLW16_07520"
     CDS             12933..13586
                     /gene="atpB"
                     /locus_tag="PLW16_07520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPB70792.1"
                     /translation="MHSLSFLANPFFPDHVTYTWFAMAVLAVLSFLATRRVSIVPGGF
                     QNVMEAIVVGFDGLLLDTMGPHGRKFFPLIATLGLFILFSNLIGLVPGFASPTASLNT
                     NAAMALVVFIMTHVVGIQIHGFKYVKQFLGPVWWLIPLMMPIEIISHLTRPLSLSVRL
                     FGNIEGGHIVIAVLLILVPFLVPLPIYLLKVLISFIQTLVFVLLSMMYIAGAMEEGH"
     gene            complement(13924..>15117)
                     /locus_tag="PLW16_07525"
     CDS             complement(13924..>15117)
                     /locus_tag="PLW16_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70793.1"
                     /translation="IDFAAILVKEEQVLDVLRQLRERRIENVLLFSSGYGETGEAGRE
                     KQRAVEDYCRREGMLLMGPNCLGYMNFPTRVSMFLGRAVEGTLRVGHVAVVGQSGATT
                     EVIVTKLLRRGLGISLYATTGNEAVMTVEDCLEYLVHDPETKVITGFFEGFRNIPKLE
                     AVARAAARRQVPILMIKVGRSAKAVQAAKSHTGALAGNDAVLDGILRQLGIIRVDTIE
                     ELAEAAHLFVHSPLPPGTGLGVCTLSGGLCGVYADLCARYGIELPPLSDETLAGLRAV
                     LPDFAQPDNPLDLTGGGFQQGVDRVVELLLNDENLAVLAPLTVPPKGPEDVFAEMINA
                     SFLSHIKTSPKPLVPIMFREAGDYARRYYEEHGIAAIEDPGLGFKAIAHLIQYARFVR
                     EFQKR"
BASE COUNT         2635 a         5134 c         4651 g         2497 t
ORIGIN      
        1 gaattgcgat cggacggcct gtctgtatcc catctcgccg ctcagcgcaa caggaaatga
       61 cgcgtgcgcc cgccgccgcc gcccttgtcc cggttatttt ccctttcgaa atatcaccgg
      121 aaccatggta tgagtgtcca tccatcccga ttccgaaggc gtgcacggga aatgtttcgg
      181 agcgggcggc cccgggatga aaaatgaagc ggagagggag acggtctcgt gaaagcggac
      241 gaattctaca tgcggcgcgc cctgcgcctg gcccggaagg gcgagtgccg ggtcagcccc
      301 aacccgatgg tgggggcggt catcgtgaag gacggccgga tcatcggtga gggcttccac
      361 gcctgctgcg gagagaacca cgcggaggtc aacgcgatcg aatgcgccat cgaggacccg
      421 gccggctcgc agttttacgt caccctcgag ccctgctgcc accagggccg gacgccgccc
      481 tgcgtcgagc ggctgatcgc cctccggccg gcgcgggtga tcgtcggcac gatcgacccc
      541 aaccccctcg tctccggacg gggaatcgcg gccctcgagg cccacgggat cgagacgaca
      601 gtcggcgtcc tcgcggacgc gtgcgctgca ctcaatgagc gcttcttcaa gttcatccaa
      661 accggccggc cgttcgtcac cctgaagttc gcccagaccc tggacgggcg gatcgccacc
      721 cggaccggcc actcccgatg gatcagctct cacccctccc tgcgcttcgc ccacgaactg
      781 cggagcaccc acgacgcgat cctggtgggc gtcggcaccg tgatcaacga cgatcctgag
      841 ctgaccgtcc gccacgtccg agggcgcaat cccctgcgcg tgatcctgga ttcgaccctg
      901 cgggtgcctc cgtcgtcccg cgtcctgtcc gcccgggacg acgcccggac gctgatcgcg
      961 acagtcggaa acgccgaccc ggaaaagcgg cggcgcctgg agcgggcggg cgtcgaggtt
     1021 ctgacggtgc cgccggacgg ggcgggccgc gtgagccttc cgcacctcct gggcctgctc
     1081 ggagagcgcc ggatttcgtc ggtcctgatc gaggggggag ccgaggtcat cacccgggtg
     1141 ctcgcggcgc ggctggccga ccggctcgtc gttgtggtcg ccccgaagat catcggccgc
     1201 ggcacggagg cggtcggcga tctgggacac gaccggatgg aacaggccct gccgctcacc
     1261 ctccgccgcc tgcggcgccg ggggcgggac accgtcttcg agttcaccgt tgaaaaaaat
     1321 ccgcaaccgg tataaacctt aatcggccgc ccgcccgaaa ttccccgtgg ccggaaagca
     1381 ggtttaccat cgtgccctgg tacgccctcc atacgcgcag ccgccacgaa gaccgcgtgc
     1441 atctcggtct gtcccaaaag gggttcaacg cctttctccc caaaatcgag gtgtggagcc
     1501 ggcgcaaaga tcgccggaaa aaaatccggc ttcccatgtt tcccgggtat ctcttcatcg
     1561 aactgacgtc actggacaac cagacgaagc tcaacgtcct gaagaccttc ggcgtcgtcg
     1621 ccctgctcgg cgccccccat caccccgacc cgatcccggt ccccgacgag aagatcgacg
     1681 ccattcagcg catcgtcgct tcccgcgtgg aggtgcagca ttttcagtac cccaaggtcg
     1741 gcgagccggc ccgcatcacc gacggcccct tcgccgggat cgagggcgtc gttctcaaga
     1801 cggatttcga gaaggaggtt ttcgtggtct ccatcgagct cctgcggcga tccgtcgcca
     1861 tccgcctcga gggcttccag atcgcccgcc tgtgatgcgt caaagcgccc ccaatgtcac
     1921 cctgagactg tcgaagggtg accgcagcct ggcttcaatc ttcgcgcttg actttgcgga
     1981 tgagttgcgc gataagcgca ggctttagtt gatctttgct gccgcccgtg ttatggaatc
     2041 gccgtcgcgg cagcgaccgt gaatgaagga accgcatcga ggagcgaccg tgccgaaggg
     2101 aaagatcacc attgataagg agttgtgcaa ggagtgccat ctgtgcgtct ttgcatgcaa
     2161 acactcaaat atcatcgccg ggaaggaata caactccaag ggctaccggc ccgtctgctt
     2221 ccaggacgac ggcaagtgca cgggatgcgc cctgtgcgcc atcacctgtc cggaaatcgc
     2281 catcgaggtc taccgtgaat agcaaggtat tgatgcaggg aaatagaatc atcggcgagg
     2341 ccgcgatccg ggccggatgc cggttttacg cgggctaccc gatcacgccg cagaacgaga
     2401 tccccgaata catggcggag aacctccgca aggtggaggg gggcgttttc atccagtcgg
     2461 agagtgaaat cgcggccatc aacatggtgg ccggcgccgc cagcgcgggg gcgcgggcca
     2521 tgacctcctc ctccggcccc gggatctccc tcaagcagga gggcatctcg tccatggccg
     2581 cccaggagct gcccggcgtc atcgtcaaca tgatgcgcgg cggaccgggc cttgggaaca
     2641 tccgcccctc ccagggcgac tatttccagg ccacgcgggg cggcggtcac ggggactacc
     2701 ggacgatcgt gctggcgccg gggacctgcc aggagttggc ggatctcacg atggacgcct
     2761 tcgatctcgc cgaccgctat cgcatccccg tggtcctgct cgccgacggc atgatcggcc
     2821 agatgatgga gccggtggtc ttcaaggaac ccaagccccg gcagtacagc gcggattacg
     2881 tcctgaaggg cgcgggcacg ggcaaggcca agaccatcaa gggcctgatc ctcgaccccc
     2941 tcgacatgga ggagcacaac tggaagctcg accggaaata ccgggtgatc gcggccaagg
     3001 aaacccgctg cgagatcttc aacacggatg aagccgacat cgtcgtcgtg gcgtacggca
     3061 ccgcggcgcg cgtcgccaaa ggggccatca agcgcctggg cgaccacggg atcaaggccg
     3121 gcctcttccg cccgatcagc ctctggccat tccccaccga ggagctgagg cggctggcgg
     3181 gcacccggaa ggaatttttc gtcttcgaga tgagcacggg acagatgctg gaggacgtcc
     3241 gcctggccct gcagggatac gcggacatcg ccttccacgg ccgtccgggc ggcgtggtcc
     3301 ccactccggt ggaactggcc aacgtgatcg tccgtcaata ccggcgcgac cggtaaggga
     3361 acttcgagag aaaaggaccg caatgaaaaa ggttttcagc agaccggaat cgttgagaaa
     3421 gaaattcgtc tttcactatt gcccggggtg cggccacagc atcgcccacc gggtcatcgc
     3481 cgagatcatc gacgagctgg gcgtccgcgg caccaccatc ggcgtgccgc cggcgggctg
     3541 cgccgtgctc gcctacaact acttcgacgt ggacatgatc gaggccatgc acggtcgggg
     3601 accggccgtg gcgacgggca tcaagcgcgt ccgtccggag tgcctcgtct tctcgtacca
     3661 gggggacggc gatctcgcgg ccatcggcat cgcggagacg ttccacgccg ccaaccgggg
     3721 cgagaacttc acgatcatct tcatgaacaa cgccgtctac gggatgacgg gcggccagat
     3781 ggcccccacc accctcctcg atcagaaatc gacgacctcg cccccgggac gcaacgccgt
     3841 cctggacggc taccccgtcc ggatcagcga gatcctggcc atgctgcccg gcacggccta
     3901 catcgagcgc tgcaccgtga actcgccggc caacatcgtc aagaccaagc gcgccatcaa
     3961 gaaggccttc cagtaccagt tggacggccg gggcttctcg ctgatcgaga tcctctcgcc
     4021 ctgtccgacg aactggaaga tgtctcccat cgacgcctgc cgctggatcg acgacgtcat
     4081 gagcaagcag ttcccgatcg gggtcatcaa ggatgagccg gcgaaagcgg ccggaaagga
     4141 gggcgcgggc catgatcgtt aagaccatct tttccggatt cggcggccag ggcgtcctca
     4201 tgatgggcta cagcttcgca cacggcgcca tgagcgaggg ctacgaggtg acctacctgc
     4261 ccgcctacgg cgcggaggtt cgtggcggca cggccaactg cacggtcgcc gtgggggacg
     4321 aggagatcgc ctcgccgatc gcgtcggagc cggactacct cgtggtcctg aacacgccct
     4381 ccctgtacac gttccagaac cggatcgccg gcggcggcct ccttttcctc aattcctcca
     4441 tcatcagcgc ccgccccagc cgtcaggaca tggaggtctt cgccgttccg gcgagcgaca
     4501 tcgccgagga actgggaaac gcccgggcgt tcaacaccgt catgatgggg gcctttctga
     4561 aacggacgag cctcgtgaag cccgagatct acctgaagag cctggagacc gtcatgggga
     4621 gcaagaagaa ggcggtcgtc gagatcaacc ggaaggcctt ctcggccggc tacgattttc
     4681 tcaaggcgtg aagcaagtaa ggcgtgaagt aaatttcgaa ggagttgacc gcagatgcca
     4741 gtgaaacaga tttccgtcct cctggacaac acaccgggca ccctggcccg gctttccaat
     4801 atcttggaca acgaggacat cacgtacaag gccgtgcttg cggcgagcgc cgaacagcag
     4861 agcaccgtcc ggctggtggt caacgacccc gaacgggcgg cggcggtcct gaagagcttc
     4921 ggcttcaact tcgaggtgag ccccgttctc gcggcggagg tgccgctgca tccgggcggg
     4981 atgaacgcca tcctgaagca cctctccgcg gcggacgtca acatccacta cctctacacg
     5041 acgatcaacc gcctgggtca ggagacgatt gtcatcctcg gggtggacaa cctcgacgag
     5101 gcccgcaagg tcctccagcg caactggatc cgcctgatcg aggacgagat ctattccctc
     5161 tgacggtcgg gcggggtctg aaatcccgag gggttaagcc ccgaacccgc tttcgtccca
     5221 tggcgatctc accggaaaaa gaacaggccc tgcgcgaccg catggcacgt ctcggcgtca
     5281 ccgaggacga gttggcggaa tccttcatcc gctcctccgg ccccggaggt cagaacgtca
     5341 acaagacgtc cacctgcgtg tacctcgtcc acaagccgac ggggctctcc gtcaagtgcc
     5401 agcaggagcg ctcccaggcg cagaaccgct tcctcgcccg gcggctgctc ctggatcgga
     5461 tcgaacggct ccagaccggc cgggtggagg ccgcgcgaca gcgcgcggaa aagatccgcc
     5521 ggcagaagcg ccgccgctct cgccgcgccc aggagaagat cctggaggcc aagcaccgcc
     5581 ggtcggagaa aaaggccctc cgcgccaaac ccgatccctc caccccttga atcggggttc
     5641 tccccttgcg cccgccccgc acccgttttt caggcaaaaa aaaggggagt tgaagtcgtt
     5701 gtctcgctcc ccctccccga ttggatatga atacgatggg ctatccgccg accacgacct
     5761 tgagcaacgg gttggcgtaa aggacgacca gcgcgatgac gagcgcgtag atcgccagag
     5821 attcgatcat ggccagaccg accatcatgg tcagcaggac cttcccctgg gcctccggat
     5881 tccttcccac ggcactggtg gcgccgctca atccgttccc catgccgacg cccgtaccga
     5941 tcgtccccat gccgatggcc agaccggcgg caagcagact gcagccgatg acgatggcct
     6001 tggtgtaatc cacgacaggc gccgccgcgg ccgcgccctg ggccgccgcc gccagcgtcg
     6061 cggtcaacaa cacgaacagc attccaacaa acgttcctac agtgactctc ttcattgaat
     6121 gtcctcccct tttttggtgt gagcctgaac accccccgac ccgccgttcc tccggcctcg
     6181 ctcacctcct ctcccggtcc tccctgaccg cctgcctcaa tcggctggcc cgccggggcg
     6241 cttgcgctcg aaaaactacc cagtgtagtt ttgcggaaac acctaacggc aagaagaaaa
     6301 aatgtcaagc attttttccc cgggcgcgac ccgctccgca tcgctttggg aacgcttgat
     6361 tttctcgtgc atccgtacta tgggatcggc agggctttcc ccaccgcgcg cctcttcggg
     6421 accgcatcgc ggttcgggcc gcgcgtgccg aaacgcgggg gaaccggaaa ggattccggc
     6481 cgtgaagacg gaccgggcgg ccggcgtcgc cgccatcctg cagaagaaca tccccctggt
     6541 gaagcgcccg ttccgcgcca tcggcgagcg tctgggtctc gccgaggcgg acgtcctccg
     6601 gatcgtccgg gagctcaaac gcgagggggt catccggaag gtaggcgcca tcctccggca
     6661 ccagccggcg ggctacgtcc acaacgccct gatcctctgg gccgtccccg acgggcggac
     6721 ggacgaggtc ggccgcatcc tggccggctt tcccgacgtc acccactgct atgaacgccg
     6781 gccgcccttc gccggcaaat acaccctctt cagcatggtc cacatgcggg agaaccggca
     6841 ggcggaactg ctcgacaccc tgacgcaagc caccggtatc accgacatcc agatcctggc
     6901 cagcgtggag gagttcaaga aaagttcgat ggagtatttc gatgagccct gaatcctcgg
     6961 aggccctgtt cgaccgggcc cggcagtgca tccccggcgg cgtgaacagc cccgtccggg
     7021 cctgcggcgc cgtgggcgcc atcccccgct tcatcgagcg ggcggcgggc gcgcacctct
     7081 acgacgtgga cgggaacgcc tatgtcgact acgtcgggtc ctggggcccc atgattctcg
     7141 gccacgcgca tccgcgcgtc acggccgcca tccaggcggc ggcggaacgg ggcacgagct
     7201 acggcgcccc gacggccgcc gaagtcgaga tggcggagct gatccgcgcg gccttccccg
     7261 cgatcgagca ggtgcggatg gtgagttccg gcaccgaggc gacgatgagc gccatccgcc
     7321 tcgcccgcgg ggtcaccggc cgggagcgga tcgtgaagtt ctccggcggc tatcacggcc
     7381 acggcgacag cctgctcgtc agggccggct cgggggtggc caccctcgga atccccggca
     7441 gccccggcat ccccgccgcc ctcgccgcgc tcacgctggt cctgccctac aacgacgccg
     7501 gggccgccga agcggccttc gcccggtgcg gcggcgagat cgccgccgtc atcgtggagc
     7561 cggtggcggg gaacatgggc gtcgtcaacc cggcgccggg atttctcgag accctgcgcg
     7621 cgctcacgcg cgcctcgggc gccctgctca tcttcgacga ggtgatcacc ggcttccgcc
     7681 tgacctgggg cggttatcag gcgctgtgcg gcatcgcgcc ggacctcacc tgcctcggca
     7741 agatcatcgg cgggggcctg cccgtcgggg ccttcggcgg ccgggccgac ctcatggcgc
     7801 acctcgcccc cgcgggggac gtctaccagg ccggcaccct ctccgggaat cccctggcga
     7861 tggcggccgg ccttgccacg ctgcgcatcc tccgggaacg tcaggacgac tatgccgtgc
     7921 tggatcgccg gaccgcgctc ctgtgcgacg agattctcac cctcttccgg cagcggggac
     7981 ggcccgcccg gatcaaccgc gccggctccc tgttcaccct cttcttcacc gacggcgacg
     8041 tgacggatta cgaaacggcc ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nngtccagat ggcccgccat ttcgacgtgg tcgggttcga catccgcccg gagcggatcg
     8221 cggaacttga ggcgggacgg gaccggaccc tcgaggtcgc cccggacgcg ctcaaggcgg
     8281 tcgccatcgc cttctcgtcg gaccccgccg tcctgaagac ctgccgcctg atcatcgtgg
     8341 ccgtcccgac ccccatcgac gaggcccgcc ggcccgacct gcggccggtc gaacaggcct
     8401 gcgtcctggt cggtcgcaac ctggcacggg ggacctgcgt cgttttcgaa tccaccgtct
     8461 accccggcgt caccgaggag gtctgcgtcc ccctcctgtc ccgggaatcg ggtttgagcc
     8521 tgggcgaggg attcaccgtg ggctattcgc ccgaacggat caacccgggc gaccgcgtgc
     8581 acaccctgga gagcatcacg aagatcgtct ccggatccga cgcggccacc ctgaagctgc
     8641 tcgtcgacgt ctacggccgg gtggtccggg cggggatcca ccccgccgcc tccatcaaga
     8701 tcgccgaggc ggcgaaggtc atcgagaaca cgcagcggga catcaacatc gccctgatga
     8761 acgaactggc gatcatcttc cagcggatgg gcatcgacac cctggacgtc ctcgcggcgg
     8821 cgggaacgaa atggaacttc ctccccttcc gcccggggct cgtcggcggc cactgcatcg
     8881 gcgtcgatcc gtactacctg acctacaagg cggagatgct cggctaccac cccgaaatga
     8941 tcttggccgg ccgccggatc aacgacaaca tgggcaagta cgtggcggaa cggaccatca
     9001 agctcctgat cggcgccggc acgcagatcc gcgaggccac catcgccgtc ctggggctca
     9061 ccttcaagga ggatgtcccg gacctcagga acacccgcgt gatcgacatc atccgggagc
     9121 ttcaggatta cggaatccgt acggtcgtcc acgacccgct ggcggacccc gacgaggtgg
     9181 agcgctactt cgggctcacc ctgcacgacc tgaaggacat cggccccgtg gacgccgtga
     9241 tcgtggccgt cgcccacgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 cacctcgtct tcgcctcgtc gagctccgtt tacggcggca acacccggat gcccttttcc
     9421 gtccacgata acgtggacca tcccgtgagc ctctacgcgg ccacgaagaa atcgaacgaa
     9481 ctgatggccc acgcctacgc ggccctctac ggcctgccct gcacgggact gcgcttcttc
     9541 acggtttacg gtccgtgggg ccggccggac atggccctgt tcctcttcac ccgtgcgatc
     9601 ctggcgggcg aacccatcga cgtctataac cacgggcgga tgcagcggga cttcacctat
     9661 atcgacgaca tcgtggaggg ggtttcccgt atcctggacc tgatccccgc gccggatccc
     9721 cggtggcggg gcgaggcgcc ggacccggcg acgagtttcg ccccgtaccg gatctataac
     9781 atcggcaaca acgccccggt ggcgctgacc gacttcatcg cggcgatcga ggcggcgctg
     9841 ggccgcaagg ccgtccggaa cccgctgccg ctgcagcccg gagacgttcc ggccacctac
     9901 gcggacgtgg acgatctgac cgccgcggtg ggattcaagc cgtcgacccc gctcgctgag
     9961 gggattggcc ggttcatccg gtggtaccgg gattactacg gcgcctgaac cgccacgcgg
    10021 cccgggcgcg ctttgccctt gacaggcccg accccgcgca actaggatgg tcgggaaata
    10081 ccccgggccg gagcaccggc ccgggggagg acaccatttc cgggtcgccc gcgacccgcc
    10141 ggaaggagag caacacggac atgaaaaagg cattcatcac cggcatcacc ggccaggacg
    10201 gatcgtatct ggccgaattc ctgctgggca agggctacga gatccacggc ctcatccgcc
    10261 ggtccagcac gttcaacacg gaccggatcg accacctcta ccgcgacttc cacgatccgg
    10321 aggcgcgggt gtatctgcac tacggcgatc tgtccgtctc cgggcagctg atggatctgc
    10381 tcggcgacat ccggcccgac gagatctacc acctgggcgc ccagagccac gtccgcgtga
    10441 gcttcgacat gcccgagtac acgggggacg tgaccggcct ggggacccta cgattgctcg
    10501 aggccatccg caagacgggc atcccggcgc gcttctacca ggcctcgtcg agcgagatgt
    10561 tcggggccgc cccgccgccc cagagcgagg ccaccccgtt ccacccccag agcccctacg
    10621 ccgcggccaa ggtctacgcc tactacatcg tgaggaacta ccgggaggcc tacgggctct
    10681 tcgccacgaa cgggatcctc ttcaaccacg agtcgccacg gcgcggcgag accttcgtca
    10741 cccggaagat cacccgggcg gcaacccgga tcaagctggg tctccaggac aagctctacc
    10801 tgggcaacct ccaggccaag cgcgactggg gcttcgcggg cgactacgtc gaggcgatgt
    10861 ggctcatgct ccagcaggag acaccggacg atttcgccat cgccaccggg gagatgcact
    10921 ccgtccagga attcgtggac cgggtcttcg cgaaactcga cctcgacccc ggccggcacg
    10981 tggccataga cccgcgttac ttccgcccca cggaggtgga cgccctgaag ggggatgcct
    11041 cgcgggcgag aacgatcctg ggctggacgc cccgggtcga cttcgacggc ctcgtggaga
    11101 tgatggtcgc ggccgacatg gaactcgcca tgcgcgagaa gacgctggtc gaggcgggct
    11161 acgcctgcaa cggcagcagc cggatgatct gagggcaacg gacgttcagg gaggccacga
    11221 tgaccgacat gcgaaaccga cggatcaccg tcaccggcgg caaagggttc ctgggcgccc
    11281 atctcctccg ggagctggac gcgaagggct gccgccgcgt ttccgtcgcc gatctgccgg
    11341 agtacaacct cgtccgcatc gaggacgtcc ggcgtctgta cgaggagcag cggccggaca
    11401 tcgtcatcca cctggccgcc aaggtggggg gcatcggctt caacgaggcc aaccccgcgc
    11461 cgctcttcta cgacaatctc atgatggggg tccagctcat gcacgagggg tccctccgca
    11521 agatcagcaa attcgtcgcg ctgggaacga tctgcgccta ccccaagttc acccccgtcc
    11581 ccttccggga ggacgacctc tggaacggct accccgagga gacgaacgcc ccctacggcc
    11641 tcgcgaagaa gatgatgctc gtccaggccc aggcctaccg gcgtcagtac gggttcaacg
    11701 cgatcttctt gctgcccgtc aacctctacg gcccggggga caacttcgac cccgcctcgt
    11761 ctcacgtgat ccccgccctg atcaggaagt gcgtggacgc gaagcgggcc ggcgccccgg
    11821 agatcgtggt ctgggggacg ggcgccgcca cccgcgagtt cttctacgtc gaggacgccg
    11881 cggaggcgat cgcgctggcg acggaaaagt acgacggtcc ggagccggtc aacatcggcg
    11941 ccggcttcga aatctccatc cgggacttga cggaactgat cgtggagctg acgaggttca
    12001 ccggccgcgt ggtctgggac gccacccgtc cggacggtca gccccggcgg atgctgaaca
    12061 ccgaccgggc ccgggacgcc ttcggcttcc aggcacggac cgccttccgg gaggggctcg
    12121 ccaagaccat cgcctggtat gaacgaaccc ggccctgacc cccggaccgg acgcgccgga
    12181 gccccgcggt aaaccggaac gggggcgctc cggttcctga ccgccggtca ggggagacat
    12241 gttttttatt tgacttcatt ccccaatcgt gctaagaggc gcggcgacaa tggatagggc
    12301 aacgagaaag tcggccattc agttggcgta cgccagcagc atcggcatcg ccatggtcat
    12361 gatggttttt ggcgggttgt ttttcgggct ctatctggac cggaagctgg acacgggcaa
    12421 caccttcacc ttcgtgtttc tcgtcatcgg tctcgtcgcc ggcttccgca acatttacgt
    12481 cctgatccgg aagaatttca cggacgaacg agatgtcatc acgtgtgtaa aaagtgaacc
    12541 ccatcgtaaa agacccactc caaaaaagac ttgaggtcac caactgggtt ctgttggccc
    12601 tgctcacgct cctgagcgcc ctgtgggcct ccccccgttt cgtcgccggg atcgtttgcg
    12661 gcgggctcat ctgcatcatt aactttcact ggctttaccg caatctgacg acggtttttg
    12721 cacagttgac cgaaaaggcg aagtcggcgg tcatgatccg ctacgtcctt cgcttcgtcc
    12781 tgacggcggc ggtgctcttc gtggtcatca cccgggagtg ggtggatatc ttcgggctgg
    12841 tcatcggcct gtctatcgtg gtcgtcaacg tcgttttgac catcgtcctc gtgtatggaa
    12901 aaaaaaatct cattcaggag gttagataac caatgcattc gttgtcattt ctggccaatc
    12961 cctttttccc ggatcacgtc acctatacgt ggtttgccat ggccgtgctg gcggtgctga
    13021 gctttctggc gacccggagg gttagcatcg tcccgggcgg ctttcagaac gtcatggagg
    13081 cgatcgtcgt cggtttcgac ggcctgctcc tggatacgat gggcccacac ggccggaaat
    13141 tcttccccct gatcgccacc ctgggtctgt tcatcctctt ttccaacctc atcggcctgg
    13201 tgcccggctt cgcgtcgccg acggccagcc tcaacacgaa cgccgccatg gccctggtcg
    13261 tcttcatcat gacgcacgtc gtgggcatcc agatccacgg gttcaagtac gtcaagcagt
    13321 tcctcggccc cgtctggtgg ctcatcccgc tcatgatgcc catcgaaatc atcagccacc
    13381 tgacgcgccc cctgtcgctc tccgtccggc tgttcggcaa catcgaaggg gggcacatcg
    13441 tgatcgccgt tctgctgatc ctggtgccgt tcctggtccc gctgcccatc tatctgctca
    13501 aggtgttgat ctccttcatc cagaccctgg tcttcgtgct cctgtccatg atgtacatcg
    13561 caggcgccat ggaggagggc cactgagaga cccggcgggg catcgtccgc tccctccccg
    13621 ggacggaccc gcccggcgcc gccgggcctg aaaccacccc acgccaccga cgaaggtcgg
    13681 agccgcacag gctccggcct ttttttgcgc tttcgacggg atgcttggtc gccgggtgct
    13741 gcataacgcg cggcatggac attcctgccc ttaggatgcc gcctgttcgc gaagatcccc
    13801 ccggccccca tcccggcgtc gctctcacgg ggatgcaccc gacgtcgtgg caaccggccg
    13861 gacacgcccg tgcttgctgc ccaaccgatg gattttcgcg aggacgggtg aaccgtcccg
    13921 ctgtcagcgc ttctgaaatt cgcgcacgaa gcgcgcgtat tggatcaggt gggcgatcgc
    13981 cttgaagccg agacccgggt cctcgatggc ggcgatcccg tgttcttcgt agtacctccg
    14041 ggcgtaatcg cccgcctcgc ggaacatgat gggcacgagc ggcttggggg acgtcttgat
    14101 gtgggacagg aaggaggcgt tgatcatttc ggcgaagacg tcttccggtc ccttcggagg
    14161 gacggtcagc ggcgccagga cggccaggtt ttcgtcgttc aggaggagtt cgacgacccg
    14221 gtccacgccc tgctggaagc cgccgcccgt caggtcgagg gggttgtctg gctgggcgaa
    14281 atcgggcagc accgcccgga ggccggcgag cgtttcgtca ctcagggggg gcagctcgat
    14341 cccgtagcgg gcgcacaggt ccgcatagac gccgcacagc ccacccgaca gcgtgcagac
    14401 gccgagtccc gtccccgggg gcagcgggct atgaacgaac aggtgcgccg cctcggccag
    14461 ttcctcgatg gtgtcgaccc ggataatccc caactggcgc aggatgccgt ccagcacggc
    14521 gtcgtttccc gcgagcgccc cggtgtgcga cttggccgcc tggacggcct tcgccgagcg
    14581 gccgaccttg atcatcagga tcggcacctg ccgccgggcc gccgcccggg ccaccgcctc
    14641 gagtttggga atattccgga acccctcgaa gaagccggtg atgaccttcg tttcggggtc
    14701 gtggacaagg tattcgaggc agtcctcgac cgtcatcacc gcctcgttgc ccgtcgtggc
    14761 gtacagggag atgcccagtc cgcgccggag cagcttggtc acgatgacct cggtcgtcgc
    14821 cccgctctgc cccacgaccg cgacatgccc cacccggagc gtgccctcca cggcccggcc
    14881 gagaaacatg ctgacccggg tggggaaatt catgtatccg aggcagttgg gccccatgag
    14941 gagcatccct tcgcggcggc agtagtcctc gacggcgcgc tgcttctccc gtcccgcctc
    15001 ccccgtttcc ccgtagccgg aactgaacaa aaggacgttt tcgatccggc gctccctgag
    15061 ctgcctcagg acgtccagaa cctgctcctc cttgaccagg atcgcggcaa agtcgat
//
LOCUS       DAOOLJ010000043        14685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4380_length_14685_cov_16.726931, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000043 DAOOLJ010000000
VERSION     DAOOLJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_14685_cov_16.726931"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /gene="rnpB"
                     /locus_tag="PLW16_07530"
     ncRNA           <1..236
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW16_07530"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(327..2738)
                     /gene="ppsA"
                     /locus_tag="PLW16_07535"
     CDS             complement(327..2738)
                     /gene="ppsA"
                     /locus_tag="PLW16_07535"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPB70794.1"
                     /translation="MKDKKLILWFDEIELNDIPQVGGKNASLGEMRRELISKGVNIPD
                     GFAVTAYAYRCLIESAALSDKIRQILADLDTHDIQNLADKGRQIRGLIYGAEFPSDLK
                     AAILEAYAKLCEEYGADTDVAVRSSATAEDLPDASFAGQQETYLNIRGPEALIEACKK
                     CFASLFTDRAISYRVDKGFDHHSIALSIGIQKMVRSDLACSGVIFSIDTESGFKDAVL
                     ITGAYGLGETVVQGIVNPDEFFVFKPTLKEGFRPILQKTIGSKEIKMIYASGSASEAT
                     EIVSVEEAERQRFCLTDDEILLLGRWAVIIEDHYSQQAGYFKPMDIEWGKDGRTDELF
                     ILQARPETVQSQKDLSVVETYVLLEEKEPLVTGTSVGAKIGAGPVNRIESVADIRNFR
                     KGQVLVTDMTDPDWEPIMKIAAAIVTNKGGRTCHAAIVSRELGVPCIVGTGNGTERLR
                     DVAEATVSCADGEVGKVYRGILKFDVSRVNAQNLSRPRTKIMMNVGNPENAFQLAGIP
                     NDGVGLARLEFIINQHISIHPMALIHFDRVTDPAVRKQIEARTRGYENRSDFFVEKLA
                     EGVGKIGAAFWPNDVIVRMSDFKSNEYANLIGGAFFEPEEENPMIGFRGASRYYDDRY
                     REAFALECAAMKKVRNVMGLTNVKLMIPFCRTVDEGKKVIAVMAERGLVQGENGLELY
                     MMVEIPSNVVLIDQFGEIFDGFSIGSNDLTQLTLGLDRDSAQVAHIFDERNQAVMDLI
                     RIAITRANKMGKKIGICGQAPSDYPEFAKFLVECGISSISLNSDTVIKTTFEILETEK
                     QVGRS"
     gene            complement(3026..4249)
                     /locus_tag="PLW16_07540"
     CDS             complement(3026..4249)
                     /locus_tag="PLW16_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPB70795.1"
                     /translation="MIANGIIKVGDTARERTVTILDQKSQEEIKVRAVNVADEVADFL
                     KDREKDLLIATILPATDAAYKEFVAKSENPLWVKKDGKIQKIYEPLYKEMAFVDKSGQ
                     ERIKVLDGKVANASQLINVSNPAATPFKEDYFTKAKTYSKGDLYISPVKGLYVNKTEF
                     EKGKRFSGIIRMATPVFDQQGFAGVVALALDVRHLAKFTDNIVPTQSGYVVEAEAASG
                     NYAFMVDNRGFIISHPNDYHIAGQYADGTPVPAITDKTSAEAMAQKGEEVLNLNQIGF
                     MDPTLQSIAREAAEGRAGMKEYQFAGRKEFVAYAPIKFYSTDYPKPAGFGWVAMNVDV
                     VKFSAYASATAKKIQSEAKSWITTIITIIIISVILLFLIMAILVRGITRSIEAEIPLE
                     AQDASKFYDDEDDDK"
     gene            complement(4385..4762)
                     /gene="atpE"
                     /locus_tag="PLW16_07545"
     CDS             complement(4385..4762)
                     /gene="atpE"
                     /locus_tag="PLW16_07545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPB70796.1"
                     /translation="MLRKFGKSLLTFFTTVAAVLVSSPVVFAAEAIPAGDSYVKVIFS
                     VGAMVGAGLAIGLGAIGAGAGIGQAASGACEAVGRNPGVQGKIMTVMLVGMAMAESIA
                     IYALVVSLVLLYANPYMKYFLAG"
     gene            5137..5307
                     /locus_tag="PLW16_07550"
     CDS             5137..5307
                     /locus_tag="PLW16_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70797.1"
                     /translation="MQEILTAVTYLVAWLICERVIEIENGLLKLIISLAAAVGVYILM
                     ALKERREKKGDD"
     gene            5357..7978
                     /locus_tag="PLW16_07555"
     CDS             5357..7978
                     /locus_tag="PLW16_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70798.1"
                     /translation="MKHKMRISIWLTTIAVIVFILLMALTVNRVREKDIVDQFSLQQL
                     ALARGTAARIEALLGNVEKNLTMLSMLPSVRTVTPDETVSSMKVIHEHLEGKVRFIAR
                     LDERGILQSVYPDRTFAELRGVRFDRYAFFQQIRKTRGPFVGELNLSEDTPLFRILGM
                     KRVFVIGVPKYRYGSRDLFTGVVFAIMPLETMIDFFVKEPKGKLAQDSWIVDHLGRLI
                     VHPVEGFTGRNVSVLEKRDDRRPGALQPDILRGRAGYGEYVLSEEGGSGERKIVAYAP
                     MRIGPQAWFMVVSTPYNRVISVVRRAFLNVMMGVAGVIIAVIIASISIDYAERRHGQM
                     KAKLKLLQERSEWQEQLVREKRTVEGIIEGLPIPAFVIDREHKIILWNKACTELTGFG
                     VQTMIGTDRHYAPFYTRKRPLIADLIIDGDIENLEKYYGTKKIRKSSTVEGAYEARDF
                     FQLTDGRSHHLYFLAAPIHDEKGEVIAAIETLQDHTREEEMSRSIKEYAETLQVELTE
                     NINLRTQIESLNNYLQSILESSPDKLYDMSGDGIVNYISHDFARGGGMTSGPVKGRHF
                     TEFMEPELREFMMQRWEEGKRGNFLPYEIEVTARDGSRRNLLITTRPLKGTDRFVVAQ
                     RDITEFKNLEKKYYESQKLAAVGQLSAGIAHEIRNPLSSIKMSLQILEKRLQPQGNDQ
                     KRFRIAQKEVEHLEHLVNDVLIFAKPSEPRKERYDVRRILEETLAMAEKGISEKAIRV
                     ATSYDEAAPPVLVDPAMLEQAFLNLIRNGIDAMEDNGTLTLRTRYAAGASPAIEIEIR
                     DEGCGIKPEDMPHLFNPFFTKKKYGTGLGLTQVKKIIELHQGTIEIQSRQGEGTRAVI
                     TLPVPQTLADGPPAASA"
     gene            8023..9405
                     /locus_tag="PLW16_07560"
     CDS             8023..9405
                     /locus_tag="PLW16_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB70799.1"
                     /translation="MPKILVIDDDRSICETLDLYLTEEGYEVKTALTGTEGLNAFVET
                     VPDLVILDIRLPDIDGFTVLEDLREEDENVKVIMITAFHDMETTIKAMKGGAFDYIHK
                     PVNVDELDMAIRKALKACEMERKIDGLLMEPSRQFKVGDIIGTGREMREIFKTVGVVS
                     QSRTTVLIQGESGTGKELIAKVIHNNTSPDEPYIAVNCSAIVETLLESELFGHEKGSF
                     TGAIARKLGKFELARSGTVFLDEISEMSVNLQAKLLRVLQEMEFERVGGKDRVRVNAR
                     IITATNKDLKALVQEGKFRDDLYYRLNIVSIHIPPLRERREDIAPLIEYLLTKINRDL
                     HKRVIGVSDEMMEVFLKYSWPGNIRELENLLIRAVVVAKGQVLGRSDFPELNEDLAAG
                     TGGIDAVPVPGAPGGRLMTLDEVEEQYIRKIISEYGDRTKGELCEILGISRPTFERKL
                     EKYKLHFERE"
     gene            9431..10033
                     /gene="yihA"
                     /locus_tag="PLW16_07565"
     CDS             9431..10033
                     /gene="yihA"
                     /locus_tag="PLW16_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPB70800.1"
                     /translation="MKITCASFVKTATRPDQYPPAVTPELAVAGRSNVGKSSLINVLL
                     NRRRLVRTSHTPGRTQALNFFTVNDRYSVVDLPGYGYAKVPEAVRRQWGPMVETYLRV
                     RETLRMVLVVMDSRREPTRDDLSLVGWLRAYRRPWLPVLTKIDKLSRSEARQAVLRFR
                     KPLDLGGGTDVIVFSARTGEGRERLWTAVEEALRSPNQVP"
     gene            10213..14610
                     /locus_tag="PLW16_07570"
     CDS             10213..14610
                     /locus_tag="PLW16_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPB70801.1"
                     /translation="MVVGSGIAGIQASLDLANSGMKVYLVENGISIGGVMAQLDKTFP
                     TNDCSACILSPKLVEVGRHPNVEIMTRRTVDSVTGEPGRFKVKLTRAPRFVNLDKCTG
                     CGDCANVCPVSVPSDFNETLNERKAIYRHFPQAIPSGFAIDKRGTSPCKAACPTHISV
                     QGYVALIAQGKFKEALKLIKRDNPFPIVCGRVCNHPCEEACKRGDVDEPIDIMHLKRF
                     VADLDLKDEHRYLPDVKEKNGKKVAVVGAGPAGLTAAYYLAIGGYAVEVFESLPVAGG
                     WLAVGIPEYRLPKDVLNAEIKVIEDTGVKIHLGKAVGKDVPFAQLKSDFDAVFIGCGT
                     VNSSKLNIPGEDMQGVIHGVDYLKRVNLGEKVFLGDKVAVVGGGNVAMDAVRTAVRTG
                     SKEVFILYRRSRAEMPASPEEIEEALEEGIKMEFLVAPKRVVGENGKVTGVECTRMEL
                     GEPDASGRRRPVEIKGSEFIVPCDALIPAIGQEADLDFVPAESGIAINKWKNFDVDPV
                     TFATNVPGVFAGGDVVTGPATVVKAVYAGKEAAVSIDRYLRGEDVKAGRECDWTKGLA
                     DPGDTSAFAKADRVRMAMLPPETRKSHFREVVEPMTEAQAVAEASRCLSCGICSECYQ
                     CVDVCLAKAIDHDMTYEEEEIEVGAVIASPGFEIFDAKIRGEFGFGVYPNVVTSIQFE
                     RILSASGPYFGHVQRISDGKEPKKVAFIQCVGSRDTSCGNSWCSSVCCMYATKEAIIG
                     KEHAKGMEPTIFFMDIRAHGKDFDRFVNRAKNEYGIRYIRSIPSTVKELQQTHNLMLK
                     YVQEDGTLVEEEFDMVVLSVGLTPPKEAAPLAKALGIELEEHGFCRTNLDNPVQTTRP
                     GVFVCGAFGGPKDIPETVMEASGAAACAGGLLASRRGTLVTEAELPAEMDMRGTGPRI
                     GVFVCHCGINIGGVVRVPEVVEYAKGLPNVVYTADNLFTCSQDTAVKMGEVIKEKNLT
                     RVVVASCSPRTHEGLFQENCEKAGLNRYLFEMANIRDQDSWVHMNEPDAATVKAKDLV
                     RMAIAKAQFLKPLKPGQLSVNHAALIIGGGLAGITAALALADQGFASYIVEKEEVLGG
                     NYRKLHYTLEGLDTREHLTRLLDRARKSELIKAYTGAEIVKIEGFIGNYKTTIRVKSE
                     EQQFEHGVVIVATGAYELKTEEYLCGQNPNVLTQRDLEELIAGEDERVKRAGSVVMIQ
                     CVGSRSPERPYCSRYCCSEAIKNALKLKEMDPGRDVTILYRDIRTFGLKEDFYKKARE
                     LNVKFVRYDEDRKPEVLADGSGLAVKVFDPILNGFIDLKADMLALSVGTAPNPGNEEI
                     GKMLKVSTNQDGFFLEAHVKLRPVDFATDGVFMCGMAHAPKLSEEAITQANAAVSRAC
                     VILTKDFIEAEGKTAYVNKSRCSACGLCEVNCPFSAIAVDLNEGCAVVNTVLCKGCGV
                     CTASCRMNAVDLNGFNNEEVLAQIAAFAM"
BASE COUNT         3067 a         4253 c         4458 g         2907 t
ORIGIN      
        1 ggtaagggtg aaaaggcgag gtaagagctc accagtttcc cgggtgaccg ggaaagctag
       61 gtaaacccca tccggagcaa gaccaaatag gaggacgtta gagggtggcc cgcccgatgt
      121 cctcgggtag cgtcgcttga ggcggccggc aacggccgtc ccagaggaat gaccatcacc
      181 ggcggacggg tgaccggagg ccgggacaga attcggctta cgcgccgctc ccgccccttc
      241 ttttcatgaa accgacgccg tccgagcggt ttcgcgcctg acagcggccc tttgcggccc
      301 ggagcttccg cttcggcgga cggtcattac gaccgcccaa cctgcttttc cgtctccagg
      361 atctcgaagg tcgtcttgat gaccgtatcc gagtttaggg agatgctcga aatgccgcac
      421 tccacgagaa atttcgcgaa ctccggataa tcgctggggg cctgcccgca gatcccgatc
      481 tttttgccca tcttgttggc ccgggtgatg gcgatgcgaa tgaggtccat gaccgcctgg
      541 ttccgctcat caaagatgtg ggcgacctgc gcggagtcgc ggtcgagtcc cagcgtcagt
      601 tgcgtcagat cgttggagcc gatcgagaac ccgtcgaaga tctcgccgaa ctggtcgatc
      661 aagaccacgt tgctgggaat ctcgaccatc atgtacagct ccaggccgtt ctctccctgg
      721 acaaggcccc tttcggccat gaccgcgatt accttcttcc cctcgtcgac cgtgcggcag
      781 aaggggatca tcagcttgac gttggtcagc cccatgacgt tgcggacctt cttcatggcg
      841 gcgcattcga gggcgaaggc ttcgcggtag cggtcgtcgt aataccgtga ggccccacga
      901 aatccgatca tcgggttttc ctcttccggt tcgaaaaagg cgccgccgat caggttggcg
      961 tattcattgc tcttaaaatc gctcatccgc acgatgacgt cgttcggcca gaaggcggcg
     1021 ccgatcttcc ccacgccctc cgccagcttt tcgacgaaga agtcgctgcg attttcgtat
     1081 ccacgggtcc gcgcctcgat ttgcttccgg accgccggat cggtgacccg gtcgaagtga
     1141 atcagggcca tgggatgaat gctgatgtgc tgattgatga tgaattccag gcgggccaag
     1201 ccgacgccgt cgttcggaat gccggcaagc tgaaaggcat tctcggggtt gccgacgttc
     1261 atcatgatct tggttctggg tcgtgacagg ttctgggcgt tgacccggct gacatcgaat
     1321 ttcaggatgc cccggtagac ttttccgact tcaccgtcgg cacaggagac cgttgcctcg
     1381 gcaacgtccc ggagccgttc cgttccgttt ccggttccca cgatgcaggg aacaccgagc
     1441 tcgcggctga cgatggccgc gtggcaggtc cgccctccct tgttcgtgac gatggccgcc
     1501 gcgatcttca tgatgggttc ccagtccgga tccgtcatgt cggtgaccag gacctggccc
     1561 ttgcggaaat tacggatgtc ggccacggac tcgatcctgt tgacggggcc ggcgccgatt
     1621 ttcgcgccga cgctggtgcc cgtcaccagg ggctccttct cctcgaggag aacgtaggtt
     1681 tccaccacgc tcaaatcctt ctgggactgc accgtctcgg gtctcgcctg gagaatgaac
     1741 agctcgtccg tccgtccgtc cttgccccac tcgatgtcca tcggcttgaa ataaccggcc
     1801 tgctgggagt agtggtcttc gatgatgacg gcccagcgtc ccaggagcag gatctcgtcg
     1861 tccgtcaggc agaagcgctg tcgctcggct tcctcgacgg aaacgatttc ggtcgcctcg
     1921 gaggccgagc ccgaggcgta gatcatcttg atttccttgc tgcctatcgt cttctggagg
     1981 atgggacgga atccctcctt cagggtaggc ttgaagacga agaattcatc gggattgacg
     2041 atcccctgga ccactgtctc gcccaggccg taggccccgg tgatgagcac ggcatccttg
     2101 aatcccgatt cggtgtcgat ggagaagatc acgccggagc aagctaggtc ggagcgcacc
     2161 atcttctgga tgccgatgga cagagcgatg gaatgatgat cgaatccctt gtccactcga
     2221 taagaaatgg cgcggtcggt aaacagggag gcgaaacatt ttttgcaggc ctcgatcagc
     2281 gcctccggac cgcgaatgtt caggtaggtc tcctgctgcc cggcgaagct ggcatccggt
     2341 aggtcttccg cggtggcgga actgcgaacg gcaacatcgg tgtcggcgcc gtattcctcg
     2401 cagagcttgg cataggcctc caggatggcg gccttcagat ccgaggggaa ttcggccccg
     2461 tagatcaggc cacggatctg tcgccccttg tcggccaggt tctggatgtc atgggtgtcg
     2521 agatcggcga ggatctgacg gattttgtct gaaagtgcgg cggattcgat gaggcagcga
     2581 taggcgtagg cggtgacggc gaaaccgtcg ggaatgttga cccccttgga aatcagttcc
     2641 cgacgcatct cccccagcga ggcattcttg ccgccgacct gggggatatc gttcagttcg
     2701 atctcatcga accagagaat caactttttg tctttcatgc acgcctcctg gggatatggg
     2761 gtcaacgggt accaaggacc gttacgggcc ggtcgtgtct agcagttttg ttttttgaag
     2821 tcaaagaaaa tgtgcggacc cgaacggatc gcgccacggg tgaatcgctg gggaagggca
     2881 ggactgtggc gggcgtggag gccattgccg aatgaatcga atcacgggtc cggggtcttt
     2941 cccggtagcg cctcctgccc cgttccaggt gggacgaacg gtaaaaaaag gggccggaag
     3001 cttgcttccg gccccgatgg gtttattatt tgtcgtcgtc ctcgtcatca tagaattttg
     3061 atgcatcctg ggcctcgagg gggatttccg cttcgatcga gcgcgtgatg ccccggacca
     3121 gaatggccat gatcaggaac agaaggatga ccgaaatgat gatgatggtg atgatcgtcg
     3181 taatccagga ttttgcctcg gactggattt tcttggccgt tgcggaggca taggcgctga
     3241 atttcacgac atcgacgttc atggcgaccc agccgaaacc ggccggtttc gggtagtccg
     3301 tcgagtagaa cttgatgggc gcataggcga cgaactcctt gcgtccggcg aactgatatt
     3361 ccttcatccc ggcgcgacct tccgccgctt cccgggcaat gctctggagg gtgggatcca
     3421 tgaagccgat ctggttgagg ttcaggacct cctcgccctt ctgggccatg gcttccgcgg
     3481 atgtcttgtc cgtgatcgcc ggtacgggcg ttccatcggc gtattgcccg gcgatgtggt
     3541 aatcgttcgg gtgagagatg atgaaaccgc ggttgtccac catgaaggcg taattgccgg
     3601 aggctgcctc cgcttcgacc acgtaaccgg attgcgtggg gacgatgttg tccgtgaact
     3661 tggccagatg cctcacatcc aaagccagcg caaccacgcc ggcgaaccct tgctggtcga
     3721 aaacaggcgt ggccatccgg atgatcccgg aaaatctctt tcccttttca aactcggttt
     3781 tgttgacgta aagtcccttg accggggaga tgtagagatc ccccttgctg taggtcttcg
     3841 ccttggtgaa atagtcctcc ttgaagggcg tggcggccgg gttgctgacg ttgatcagct
     3901 ggctcgcatt cgcgaccttt ccgtccagca ccttgatgcg ctcctgcccg gatttgtcta
     3961 cgaaagccat ttccttgtag agcggttcat agatcttttg tatcttcccg tccttcttca
     4021 cccaaagggg gttctcgctt tttgcgacga attctttgta ggctgcatcc gtggcgggca
     4081 agatggttgc gatcaggaga tccttttccc ggtccttcag aaaatcagca acttcatccg
     4141 ccacgtttac agcccgcacc ttgatttctt cctgggactt ctggtccaga atggttaccg
     4201 tccgttccct tgcggtgtcc ccgactttta taatgccatt ggcaatcaag agggccatca
     4261 aagataaggg gatgacgata agcaggaaga atacccttcc gactgtgaat aactgcttat
     4321 ctttattaat ttttggcatt cccttttccc cctactgttt tagtctcgaa gtgatgctga
     4381 cggattaacc ggccagaaaa tacttcatgt aggggttggc gtaaagaagt accagtgaaa
     4441 cgaccagggc gtagatcgcg atcgattccg ccatggccat accgaccagc atcaccgtca
     4501 tgatttttcc ctgaacgccg gggtttcttc cgaccgcttc acaagccccg cttgccgcct
     4561 ggccgatacc ggcgccggcg ccgatggcgc ccagaccgat ggccaaaccc gcaccgacca
     4621 tcgccccaac ggaaaagatg accttcacat acgaatcacc cgctggaatg gcttcagccg
     4681 cgaacacaac cggtgaagac accaacacgg ctgcgaccgt cgtgaagaac gtcaataaac
     4741 ttttcccgaa tttccttaac attttcctat ctcctttccg aaataatgtt tttaccttcc
     4801 gaccgatacc atactctgat tgttcggtac agaagaattc acttccgcaa atgttgcgag
     4861 cctatcgcaa aatgcatgcc gcccacggca cccgtggggt aatctccgtc aaatgcaagc
     4921 aatgattcat caattaattt ccacgacccc gcccttggga atcgagttgc cttccgggct
     4981 tgaacgttcc tttaaacccg tcccgaccgt tgaacgaaaa gtaaaagggc ggggcgggcc
     5041 gagaggatga aagacgcgat cttttctatt gaaattttcc aactgcaccg gtactatcga
     5101 gcgcccaaaa cttgaattcc attaagaggt ttaagaatgc aggaaatcct gacggccgtg
     5161 acctatctgg ttgcatggct gatatgtgag cgtgtgatcg agattgaaaa cggtctgctc
     5221 aagttgatca tctcgcttgc agcggctgtc ggcgtctata tcctgatggc tttgaaggag
     5281 cggagggaaa agaagggaga tgactgagga ggagcggacg cagtgaggtc cggtgaggct
     5341 gaggagtcct gacgtgatga aacataaaat gcgcatttcc atctggctga cgacgattgc
     5401 ggtcatcgtg ttcatcctgc tgatggccct cacggtgaat cgggtacggg aaaaagatat
     5461 cgtcgatcag ttcagcctgc agcaacttgc cctggccagg ggtacggcgg ctcgcatcga
     5521 agccctgctg ggcaacgttg aaaagaatct gaccatgctg tccatgctgc cgagtgtgcg
     5581 gacggtgacc cccgacgaaa cggtttcaag catgaaggtc attcacgagc acctcgaggg
     5641 gaaggtccgc ttcatcgcac gccttgatga gcgggggatc ttgcagtccg tttacccgga
     5701 tcggaccttc gccgagctgc ggggggtacg ttttgaccgg tacgcctttt ttcagcagat
     5761 ccggaaaacg agaggcccct tcgttggaga actgaacctt tccgaagata ccccgctgtt
     5821 ccggattctc gggatgaagc gtgttttcgt gatcggcgtg cccaagtacc gctacggaag
     5881 ccgggatctc ttcaccgggg tggttttcgc gatcatgccc ctggagacga tgatcgactt
     5941 cttcgtgaag gagcccaagg gaaagctggc gcaggattcc tggatcgtcg atcacctggg
     6001 acggctgatc gtccatccgg tggaggggtt caccgggcgt aacgtgagcg tcctcgaaaa
     6061 gagagatgat cggcgcccgg gtgcgctcca gcccgacatt ctgcggggcc gggcgggcta
     6121 cggggaatac gtcctgagtg aagaaggtgg ttccggggaa cggaagattg tcgcgtacgc
     6181 cccgatgcgc atcggtccgc aggcctggtt catggtggtc agcactccat acaaccgcgt
     6241 catctccgtc gtcagacgcg ccttcctcaa cgtcatgatg ggcgtggccg gcgtgatcat
     6301 cgcggtgatc atcgccagca tctccatcga ttatgcggag cggcggcacg gtcagatgaa
     6361 ggcgaagctc aaactgctcc aggaacggag cgaatggcag gagcagctcg tccgggaaaa
     6421 gcggaccgtg gaagggatta tcgaaggcct gcccattccc gccttcgtca tcgaccggga
     6481 acacaagatc atcctctgga acaaggcttg tacggagctg accgggttcg gcgtgcaaac
     6541 gatgatcggc accgatcggc actacgcgcc gttctatacc cggaaacgcc ccctgatcgc
     6601 cgacctcatc atcgacggag acatcgagaa tctggagaaa tactacggga cgaagaaaat
     6661 caggaaatcc tccaccgtcg agggggccta cgaggcccgc gactttttcc agttgacgga
     6721 cggcagaagc caccatctgt acttcctcgc cgcgccgatt cacgatgaaa aaggcgaggt
     6781 catcgccgcc atcgaaaccc tccaggatca tacgcgggag gaggaaatgt cccgcagcat
     6841 caaggagtac gccgaaacgc ttcaggtcga acttacggaa aacattaatt tgcgcacgca
     6901 gatcgagtcc ctgaacaatt acctccagtc catcctggaa agctccccgg acaagctcta
     6961 cgatatgtcc ggcgacggga tcgtcaatta tatcagccat gattttgccc gtgggggcgg
     7021 catgacgtcc ggacccgtga aaggtcgcca ctttaccgag ttcatggaac ctgagctccg
     7081 ggaattcatg atgcagcgct gggaagaggg aaaacggggc aacttcctgc cctacgaaat
     7141 cgaggtcacc gcacgggacg ggtcgcggcg caacctcctg attaccacgc gccccctcaa
     7201 gggcacggat cggttcgtcg tggcacagcg cgacatcacg gaattcaaga acctggaaaa
     7261 gaagtattat gaaagccaga agctggcggc ggtcggtcaa ttgtcggcgg gcatcgcgca
     7321 cgagatccgc aatccgcttt cctccatcaa gatgagtctg caaatcctgg agaagcgcct
     7381 gcaaccgcag ggaaacgatc agaaacggtt ccgcatcgcc cagaaggagg tcgagcacct
     7441 cgaacatctg gtcaacgatg tgctgatctt tgcgaaaccc tccgaacccc gcaaggagcg
     7501 gtacgacgtt cgccgcattc tcgaggaaac cttggccatg gcggagaagg ggatcagcga
     7561 gaaggcgatt cgcgtcgcca cgtcttacga cgaggccgcg cctccggttc tggtggatcc
     7621 ggccatgctc gaacaggctt tcctgaacct catccgcaac gggatcgacg ccatggagga
     7681 caacggtacg ctgaccctcc gtacgagata tgccgccgga gcgtcgcccg cgatcgaaat
     7741 cgagattcgc gacgaggggt gcgggatcaa accggaggat atgccgcacc tgttcaatcc
     7801 gttcttcacg aagaagaaat acggaacggg attggggctg acgcaggtca agaaaattat
     7861 cgaactgcat cagggcacca ttgaaatcca gagcaggcag ggcgagggga cgcgcgcggt
     7921 catcacgctg cccgtcccgc agacgctcgc ggatggtcct ccggccgcgt cggcatgagg
     7981 atgtcgtcac accaccggat catggaccat gaggagtgag gaatgccgaa aattcttgta
     8041 attgacgatg accgctcgat ctgtgaaacc ctggatctgt acctcaccga agaggggtac
     8101 gaggtcaaga ccgccctcac gggaaccgaa ggactgaacg ccttcgtcga gaccgttcct
     8161 gacctcgtca tcctcgacat tcgtcttccg gacatcgatg gttttaccgt gctcgaggac
     8221 ctgcgcgagg aggatgaaaa cgtcaaggtg atcatgatca ccgcatttca cgatatggaa
     8281 accaccatca aggccatgaa gggtggggcc ttcgactaca tccacaagcc ggtcaatgtg
     8341 gatgaactgg acatggccat tcgaaaggcg cttaaggcgt gcgagatgga gcggaagatc
     8401 gacggtctgc tcatggagcc atcgcgccag ttcaaggtcg gcgacatcat cggaacgggc
     8461 cgcgagatgc gcgagatttt caagacggtc ggcgtcgttt cccagagccg cacgacggtc
     8521 ctgatccaag gcgaaagcgg aaccggcaag gagctgatcg ccaaggtgat tcacaacaac
     8581 acctcgcccg atgaacccta catcgccgtc aactgctccg ccatcgtcga aaccctgctc
     8641 gaatcggagc tgttcggtca tgaaaagggg tcgttcacgg gtgcgattgc ccgaaaactg
     8701 gggaagttcg aactcgcccg gtccggaacg gtgttcctgg acgagatcag cgagatgtcg
     8761 gtcaacctcc aggcgaaact cctgcgcgtc ttgcaggaga tggagttcga gcgtgtgggc
     8821 ggcaaggacc gggtccgcgt caatgcccgc atcatcaccg cgacgaacaa ggatttgaag
     8881 gcgctggtcc aggagggcaa gttccgcgac gatctttact atcgcctcaa catcgtttcc
     8941 atccatatcc ctccgctccg ggagcgacgc gaggacattg cgcccctcat cgaatatctg
     9001 ctgacgaaaa taaaccggga tctgcacaag cgcgtgatcg gcgtgtcgga tgaaatgatg
     9061 gaggtttttc tgaagtattc ctggcccggc aatatacggg agcttgagaa tctcctgatc
     9121 cgtgccgttg tggtcgccaa ggggcaggtc ctgggccggt ccgacttccc ggagctcaac
     9181 gaagatctcg ccgccggcac ggggggcatc gatgcggtgc cggtgccggg cgccccaggc
     9241 gggagattga tgaccctgga cgaggtcgag gaacagtaca tccggaagat catctccgag
     9301 tacggggacc gaaccaaggg ggagctgtgc gagattctcg ggatctcccg cccgacgttc
     9361 gaacgcaagc tggaaaagta caaattgcac ttcgagcggg aatgacccgc gatccgattc
     9421 cctgggcaca atgaagataa cctgcgcatc tttcgtcaag acggccaccc ggcccgacca
     9481 gtaccccccc gccgtgaccc cggagttggc cgtggcaggc cgatccaatg tcggcaaatc
     9541 ctccctgatc aacgtcctcc tgaaccggag gcggctcgtc cggacgagcc atacgccggg
     9601 acgaacgcag gccctaaatt tcttcaccgt caatgatcga tattccgttg ttgatcttcc
     9661 cggttacggc tacgcgaaag tgccggaagc ggtccggcgt cagtggggac cgatggtgga
     9721 aacgtacctc agggtgcgcg aaacgctcag gatggtcctc gtcgtgatgg acagccgccg
     9781 ggagccgacc cgggacgacc tctccctggt cggatggctc cgggcctacc gccgcccctg
     9841 gctgccggtc ctgacgaaaa tcgacaagct gtcccggagc gaggcgcggc aagccgtcct
     9901 ccgcttccgg aaacctcttg acctgggtgg cgggacggat gtcatcgtat tttccgcccg
     9961 gactggcgag ggacgcgagc gcctctggac ggccgtcgag gaggccctcc ggtcccccaa
    10021 tcaggtcccc tgaggggaag gggcgcgcag gtctgaacac ggatgttcac gcttgacagc
    10081 ccggggaaat cgtgataacc gctgccacgc tttgagaaca atcactgacc gtgggtcagt
    10141 tttggggatg atcaataaac attgccgagg agcagacagg tggtaaaaga taacgagaaa
    10201 atcggtgccg tgatggtcgt cggttccggc attgccggca ttcaggcttc cctggatctc
    10261 gccaattccg gtatgaaagt atacttggtg gagaacggaa tcagcatcgg cggggtcatg
    10321 gcgcagttgg acaagacctt ccccaccaac gactgttccg cctgcatcct ctctccgaaa
    10381 ctcgtggagg tcggacggca tccgaacgtg gagatcatga cccgccggac cgtggactcc
    10441 gtgaccggcg agcccggccg attcaaggtc aagcttacgc gggccccccg tttcgtcaac
    10501 ctggacaaat gtacggggtg cggtgattgc gccaatgtct gtccggtatc cgtcccgtcg
    10561 gatttcaacg agacgctcaa cgaacgcaaa gccatctacc gccacttccc ccaggcgatt
    10621 ccgagcggat tcgcgatcga caaacgcggg acttccccct gcaaggccgc ctgccccacc
    10681 catatcagcg tccaggggta cgtggcgctg atcgcccagg ggaagttcaa ggaagccctc
    10741 aaactcatca agcgggacaa tccctttccg atcgtttgcg gccgcgtctg caaccatccc
    10801 tgcgaagagg cttgcaagcg gggcgatgtc gacgaaccca tcgacatcat gcacctgaaa
    10861 cggtttgtcg cggatctcga cctgaaggac gagcaccgct atctcccgga cgtgaaggaa
    10921 aagaacggca agaaggtggc cgtggtcggg gccgggccgg cgggactcac ggcggcctac
    10981 tatctcgcca tcgggggcta tgccgtcgag gtcttcgaat cgctgccggt ggcgggcggc
    11041 tggctggccg tcggcatccc cgagtaccgt ttgccaaagg acgtgctcaa cgcggaaatc
    11101 aaggtcatcg aggacacggg ggtgaagatc cacctgggca aggccgtcgg caaagacgtc
    11161 cccttcgcac agcttaagag cgacttcgac gccgtcttca tcgggtgcgg cacggtgaac
    11221 agcagcaaac tcaacattcc cggcgaggac atgcaggggg tgatccacgg tgtcgattac
    11281 ctgaagcgtg tgaacctggg agaaaaggtc tttctggggg acaaggttgc cgtcgtcggc
    11341 ggcgggaacg tggccatgga tgccgtccgc acggccgtcc gcacgggttc caaggaggtc
    11401 ttcatcctgt atcggcgctc ccgcgcggag atgccggcct cgcccgagga gatcgaggag
    11461 gccctcgaag aggggatcaa gatggagttc ctcgtggcgc ccaagcgcgt cgttggcgag
    11521 aacggcaagg taacgggcgt ggaatgcacg cggatggaac tgggagagcc tgacgccagc
    11581 ggccgccgcc gtcccgtcga gatcaaaggc tccgaattca tcgttccctg cgatgccctg
    11641 attcccgcca tcggccagga ggccgacctc gacttcgtcc cggccgaaag cggcatcgcg
    11701 atcaacaagt ggaaaaattt cgacgtcgac cccgtgacat tcgccacgaa cgtgcccggc
    11761 gtcttcgccg gcggtgatgt cgtcaccggt ccggccaccg tggtcaaggc cgtctatgca
    11821 ggcaaggaag cggccgtttc gatcgaccgc tatctccgcg gcgaggacgt caaggccggc
    11881 cgggagtgcg actggacgaa gggtctggcc gatccgggtg atacgagcgc gttcgcgaag
    11941 gcggacaggg tgcggatggc gatgctaccc cccgagacga gaaagtccca cttccgggag
    12001 gtggtcgagc ccatgacgga ggcgcaggcc gtcgccgaag caagtcgctg cctctcctgc
    12061 gggatctgct cggagtgcta ccagtgtgtg gacgtctgtc tggccaaggc catcgatcac
    12121 gacatgacgt atgaggaaga ggaaatcgag gtcggggcgg tcatcgcctc acccggtttc
    12181 gagattttcg atgcgaagat ccggggcgag ttcggcttcg gcgtctatcc gaacgtcgtc
    12241 acgagcatcc agttcgagcg gatcctttcc gcgtccggtc cgtatttcgg ccatgtccag
    12301 aggatctccg acggaaagga gccgaagaag gtggccttca tccagtgtgt cggttcccgt
    12361 gacacgtcct gcggcaacag ctggtgctcc tccgtgtgct gtatgtatgc gaccaaggag
    12421 gccatcatcg ggaaggaaca tgccaagggg atggaaccga ctatcttctt catggacatc
    12481 cgggcccacg gcaaggactt cgaccggttc gtcaaccggg cgaagaacga gtacggcatc
    12541 cgctacatcc gctccattcc ctccaccgtc aaggagttgc aacagacgca caacctgatg
    12601 ctcaagtacg tccaggagga tggaacgctg gttgaagagg aattcgacat ggtggtcctg
    12661 tccgtcggtc tgacgccccc gaaggaggcg gcgcccctcg ccaaggccct cgggatcgag
    12721 ctggaggagc acggtttctg caggacgaac ctggacaatc ccgtccagac gacgcgtccg
    12781 ggtgtcttcg tctgcggcgc cttcggcggt cccaaggaca tcccggagac cgtcatggag
    12841 gccagcggcg ccgccgcctg cgcgggaggg ttgctcgcgt cccgtcgagg caccctggta
    12901 acggaggcag agctccccgc ggagatggac atgcgcggca cgggtccccg catcggcgtc
    12961 tttgtctgcc actgcggtat caacatcggc ggcgtcgtgc gcgtccccga agtcgtcgag
    13021 tacgccaagg ggctgcccaa tgtcgtctat acggccgata atctattcac gtgctctcag
    13081 gacaccgccg tgaagatggg cgaagtcatc aaggaaaaga atctgacccg cgtcgtcgtt
    13141 gcctcctgct cgccccggac ccacgagggt cttttccagg aaaactgcga gaaggcgggg
    13201 ctgaaccgct acctcttcga gatggccaac atccgggacc aggattcctg ggtgcatatg
    13261 aacgagccgg acgcggcgac cgtaaaggcg aaggacctcg tgcgtatggc gatcgcgaag
    13321 gcgcagttcc tgaagcctct caaaccgggt caactcagcg tcaaccatgc cgccctgatc
    13381 atcggcggag gactggccgg catcaccgcc gccctggcgc tcgcggatca gggtttcgcc
    13441 tcctatatcg tggagaagga ggaggtcctg ggcgggaatt accggaagct ccattacacc
    13501 ctcgagggtc tcgatacgcg ggaacatctg acgcggctgc tcgaccgagc ccggaaatcg
    13561 gaattaatca aagcctatac cggcgcggaa atcgtcaaga tcgagggttt catcggcaac
    13621 tacaagacca cgatccgggt gaaatccgaa gagcagcagt tcgagcacgg cgttgtgatc
    13681 gtggcgacgg gggcctatga gctgaagacg gaggagtacc tctgcgggca gaacccaaac
    13741 gtcctgacgc agcgcgatct ggaggagctg atcgccggag aggacgagcg agtgaagcgc
    13801 gccggcagcg tggtcatgat ccagtgcgtc ggctcgcgtt ctcccgagcg gccctattgt
    13861 agccgctact gctgcagcga ggcgataaag aacgccctca agctgaagga aatggatccc
    13921 ggccgggacg tcaccatcct ctaccgtgac atccggacct tcgggctcaa ggaggacttc
    13981 tacaagaagg cgcgggaact caacgtgaaa tttgtccgct acgacgagga ccgcaagccc
    14041 gaggttcttg ccgacggatc ggggctggcc gtcaaggttt tcgatcctat tctaaacgga
    14101 ttcatagatt tgaaggccga catgttggcc ctgagcgtcg gtacggcacc taatcccggc
    14161 aacgaagaaa ttgggaaaat gctcaaagtg tcgacgaatc aggacgggtt tttcctcgag
    14221 gcccacgtaa aactccgccc ggtcgatttt gcgacggacg gtgtcttcat gtgcggtatg
    14281 gcgcacgcgc cgaagctcag cgaggaggcc atcacccagg ccaacgccgc cgtgtcccgc
    14341 gcctgcgtga tcctgaccaa ggacttcatc gaggcggagg ggaagacggc ctacgtcaac
    14401 aaatcccgtt gctcagcctg tgggctgtgc gaggtgaact gtcccttcag cgccattgcg
    14461 gtggacttga acgagggctg tgccgtcgtc aataccgtcc tctgcaaggg ctgcggggtt
    14521 tgtacggcgt cctgccgcat gaacgccgtg gatttgaacg gcttcaacaa cgaggaggtc
    14581 ctggcgcaga tcgccgcctt cgcgatgtaa ctaagactcc atggagaaat tatgtctgag
    14641 aatcaagcac cgtccagctg ggaaccgaag atcgtcgcca ttgtc
//
LOCUS       DAOOLJ010000044        14439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4502_length_14439_cov_16.928115, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000044 DAOOLJ010000000
VERSION     DAOOLJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4502_length_14439_cov_16.928115"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLW16_07575"
     CDS             complement(<1..416)
                     /locus_tag="PLW16_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70802.1"
                     /translation="MGVRLAKTAGFCMGVKRAVDMVLDLAPPRGKEKLYTYGPLIHNP
                     QTIELLKLRGIAPIERLDDMDTLNGPATIIIRAHGISPRERREIKACGIRIIDATCPK
                     VAHVQAIIQKHAARQYHIMIIGDRSHPEVNGLLGYAG"
     gene            complement(406..1170)
                     /locus_tag="PLW16_07580"
     CDS             complement(406..1170)
                     /locus_tag="PLW16_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPB70803.1"
                     /translation="MAEKRIRLDRLLVERGLCPSRERAQALILSGAVRVGEDLVDKAG
                     AMIPAAAAIRLEGQDQPYVSRGGLKLAGALAVWPLCVQGCVALDVGASTGGFTDCLLQ
                     AGAARVYALDVGYGQLAWKLRADPRVVCLERINIRHFNGQGLDPPPDLATIDVSFISL
                     KLVLPVVERLVVGGGSLLALIKPQFEVSRGEVGKHGVVRDPDLHARVIEDIVACARGL
                     GLAVVGTCPSPLLGPAGNREFFLLARKRVSEASHGC"
     gene            complement(1179..3089)
                     /gene="dxs"
                     /locus_tag="PLW16_07585"
     CDS             complement(1179..3089)
                     /gene="dxs"
                     /locus_tag="PLW16_07585"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPB70804.1"
                     /translation="MKEKDIQTATWLDRIDSPEDIRRLDTEQLNRLAEEIRERIIRTV
                     ARTGGHLASSLGTVELTLAIHYVFNTPQDKLVWDVGHQAYAHKIITGRRDRFATLRQQ
                     GGLSGFPRREESPYDVFNVGHSGTSISAAAGIAEAQCLNRQPGKVIAVIGDGSMTSGI
                     ALEAFNWAGDRKKNLIILLNDNEMSISPNVGAMSAYLNRVMTGQAVTKLRAEIKGFLK
                     TIPGIGVQMVKFTRQLEESLKAFIVPGALFEDLGFTYVGPLQGHRLDRLIRTLQNVRE
                     MEGPILVHVLTTKGKGYAFAETEPLKFHGISTFDAETGHSMAVADPTPTYTEVFGRTL
                     VRLAGEDSRIVAITAAMCEGTGLTGFAREYPHRFFDVGIAEQHAVTFAAGLATEGLIP
                     VVAIYSTFLQRAYDQIIHDVCLQNLPVVFALDRGGLVGDDGATHQGMFDLSYLRSIPN
                     LIVMAPRDENELQRMLQTAVSCGRPAAVRYPRGKAIGVPLEMPCRPLDIGRGEIVAEG
                     GDLAIIAVGMMVQPALAAAARLQKEGVSARVIDARYVKPLDEALLCTAAADTGKVLVV
                     EENVLMGGFGSAVLELFARDGIGPVRVQRLGIADEFVEHATQAQQRRRYGLDEDGIVA
                     ASRRLMNAASPP"
     gene            complement(3111..4034)
                     /locus_tag="PLW16_07590"
     CDS             complement(3111..4034)
                     /locus_tag="PLW16_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB70805.1"
                     /translation="MDDGKRDNSEALKLQAYLKEKQSRVEEALARFLPAEDAFPPVIF
                     RAMRHSVLAGGKRLRPILCMAAAEAVGGKAETVLPVACALEFIHTYSLIHDDLPAMDD
                     DDLRRGRPTSHKVFGEDIAILAGDALLTEAFHLLSRPEGRAALPPEQRLAVIHDVAAA
                     AGFRGMVGGQVVDVQSAGKAMDRETLHYIHTRKTGAMIVVSVRAGAVLAGAADNAVAA
                     LTAYGEQAGLAFQIADDILNVEGDARRLGKTTGSDAARGKITYPALLGLAAAKRKTRR
                     LVRQAITSLKDFDQRADPLRWIATYIIQRQS"
     gene            complement(4040..4273)
                     /locus_tag="PLW16_07595"
     CDS             complement(4040..4273)
                     /locus_tag="PLW16_07595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPB70806.1"
                     /translation="MAKDKFEDALHKLEALVRKMESGELSLEESLKAFEEGIRLSRQC
                     AQKLDEAERRVEVLLRDGDDLAVRPLPAADVED"
     gene            complement(4277..5617)
                     /gene="xseA"
                     /locus_tag="PLW16_07600"
     CDS             complement(4277..5617)
                     /gene="xseA"
                     /locus_tag="PLW16_07600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPB70807.1"
                     /translation="MKEILTVTTLTERIRELLEDGFGIVWVEGEVSNLRRPASGHQYF
                     TLKDDRSQIRGVLFRSAFAPERANRFSLEEGMGVVCRGRVSVYPPRGDYQLIVDHVEP
                     LGLGALQKAFEQLKSRLEAEGLFDAARKRPLPFLPHRIAVITSPTGAVIRDILNVTGR
                     RFPSVPILLVPVRVQGPEASAEIIRALQDVQTRADVDCVILARGGGSLEDLAPFNDEG
                     VARAIARCRLPVVSAVGHETDFTIADFVADLRAPTPSAAAELAVPVRNELQGTVDALA
                     RRLIRQQELCLRGFRERVRAAGDRLRHPGRVIGDHRLTVDDFSERLRRGLRQRAAAQA
                     AAWSGLRTRLSQRDPRQTVREKRLLSGDYRRRLEQGFQSLLARSRERLKAGTALLGSL
                     SPLSVLNRGYGIVRRLPDGAILRRADAVAAGGEVAVQLSRGRLTARVLKIEQEQ"
     gene            5808..6824
                     /locus_tag="PLW16_07605"
     CDS             5808..6824
                     /locus_tag="PLW16_07605"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPB70808.1"
                     /translation="MKKKDYLKQPIRHVDMKAIDATSIIDAMKEMSFSARDLAAAAEI
                     YDRMLADRKCAIILTIAGSTSAGGCMQIYADLVRHHMVDAVVATGASIVDMDFFEALG
                     FRHYRGTPQVDDRELRRLYIDRIYDTYINEEELQACDRTVKAIADALPPRPYSSREFI
                     REMGRHLTVHAKKKDSLVQGAYEEDVPIFCPAFSDSSAGFGLVLHQHERPDQHVSIDS
                     VKDFLELTKVKIAAKTTGLLMIGGGVPKNFAQDTVICAEVLGKDVPMHKYAIQITVAD
                     VRDGACSSSTLKEAASWGKVDVTHEQMVYAEATSVLPLLASYAYHRGNWKKRRHWAFS
                     KLFA"
     gene            complement(6846..7877)
                     /locus_tag="PLW16_07610"
     CDS             complement(6846..7877)
                     /locus_tag="PLW16_07610"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70809.1"
                     /translation="MASATASGLRIGMTYDLREDYLAQGFGDEETAEFDHPETIRALA
                     DALEIAGHGVDRIGPLRSLVRRLVDGNRWDLVFNIAEGLHGFGREAQVPALLEAYGIP
                     YTFSDALVMALTLHKGLTKHILRDRGLPTPAFVVIENHADLAAPNLSFPLFVKPVAEG
                     TGKGVTGASKVNSPDALARVCRTLLKNFRQPVLVEEFLPGREFTVGIIGTGRAAEVVG
                     VMEVQWLSQAEGDVYSFANKEHWEDRVVYRLAGDKEADAAGEVALAAWRVLGCRDAGR
                     VDLRSDARGNPNFIEVNPLAGLRPGYSDLPILCGLAGITYQTLIERIVASALARHPAT
                     GSKRTAEPL"
     gene            complement(7924..9225)
                     /locus_tag="PLW16_07615"
     CDS             complement(7924..9225)
                     /locus_tag="PLW16_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB70810.1"
                     /translation="MEIEALVSEEVEPPGPLTAAFSVAEPALNPAWTDARAVTDPPSP
                     LTRKGRNDLAESPGRLFRRRFFPEATPGDWNNWNWQFRNRIGTADVLDRVLRLTEDEK
                     SAISRHAGLLPVAVTPYYASLLDADNPQQPLRRTVIPTNDEFLRTAGEADDPLEEERD
                     SPVPGIVHRYPDRVLFLVTGLCSTYCRYCTRSRMVGTAKYGFSHDQWGEGLTYIRSHA
                     EVRDVLLSGGDPLTLADENLAWLLAELHRIPHVEMIRIGTKAPVVMPQRITPALVSVL
                     KKFHPLWMSLHVTHPEELTPEMGRACARLANAGIPLGSQTVLLSGINDDPVTMKTMFQ
                     GLLHLRVKPYYLYQCDPIPGSSHFRTPVAKGLEIIERLRGHTTGYAVPTYVIDAPGGG
                     GKVPLLPRYCLGRQGDDLLLKNYLGQVYRYPDHPVSGWDIH"
     gene            complement(9269..9520)
                     /locus_tag="PLW16_07620"
     CDS             complement(9269..9520)
                     /locus_tag="PLW16_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70811.1"
                     /translation="MACKKGENLVTVTGIVIPVDWKGDRVVAAAIATAREEEYFIARN
                     RKGGELLKFLQEPIEAEGVVGTDEDGRMTIRVKRYTVKS"
     gene            9913..10698
                     /gene="mutM"
                     /locus_tag="PLW16_07625"
     CDS             9913..10698
                     /gene="mutM"
                     /locus_tag="PLW16_07625"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPB70812.1"
                     /translation="MPELPEVETLRRQLNPLIAGETIRGLEILDEKLRGIRDPAGKTI
                     LALCRRGKYLEFHLDDGATLVLHLRMSGRLRWLTGPGVFPHTRFRFSFDSGRLIGIDP
                     RRFATLGSLSDRPAPSPIADPSQSIRAGKWMDIAAGRRLPVKSFLLDQRLVAGIGNIY
                     ACEILHAAGIHPGRPSGSLTKGEWIGIAAAAKAILRDAVACRGTSISDWRDLFGRPGV
                     YQHRLKVYGKAGKACPRCAATVVRIRLGGRGTYFCPICQTPSR"
     gene            complement(10753..11862)
                     /locus_tag="PLW16_07630"
     CDS             complement(10753..11862)
                     /locus_tag="PLW16_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB70813.1"
                     /translation="MLDLNNFIIAGRFFLVIAGELILIFVAVSFLIGLLTEYLPPSRI
                     RDYLAGKLSLVQYVLGAGLGALTPFCSCSTVPITAGLLKGGVPFGPVMAFLFASPVLN
                     PFIVALLLSLFGVNVTVFYVVVTFFGSMAVATLMSRMGMEDQVKPLIGLESTCCSPAK
                     KPVTGTVKIDAAGPGFYAPVGASSLSMNLPMFAAVDDCCSSSVQESVQESAAPCCSVD
                     FSTDGKTGPAGAPVSEKMKRASRSAIVTFKGVFWYLLLGAGIGAFIYGFFPQDLIVRL
                     AGPGNPWSIPIAALIGVPMYIRAETIIPISAALVGKGMGLGTVLALIVGGAGASIPEL
                     IILGSMFRKKLIVTFVLNVFLVAMIAGYLVDLVLY"
     gene            complement(11895..12221)
                     /locus_tag="PLW16_07635"
     CDS             complement(11895..12221)
                     /locus_tag="PLW16_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPB70814.1"
                     /translation="MSAGPNHGYGILKALGSLTSGCCIPTLGSIYPILKELTRHGYAE
                     VREDRQLKGAQKRRVYTLTPAGLEAYRVALEAWRSSMPYIYRAIGGAASGPVQEESVP
                     CPGEKS"
     gene            complement(12663..13538)
                     /locus_tag="PLW16_07640"
     CDS             complement(12663..13538)
                     /locus_tag="PLW16_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB70815.1"
                     /translation="MRNLFFAIVINSGIVAFEMIFGLLIQSMALISDAIHNLSDIAHM
                     LFSYWAEKVARRPANNRKTYGYRKIEFIAAFVNSIGLSVVIAVVFWETLKRFTTPTEV
                     PGQVMLWVGVAAFAGNTIVTLLLQKMSARNINLKSVWLHSFQDALFSAGVIAGALLIT
                     FFGWHFIDPIISLVICFILAREIYKLIRQTVNSLLDSVPPGIDFAEVRNDLLAIPEVS
                     EVNDLHIWQTGTDQKLLSAHLVSAEDSPDHEAIIRTAHKILMRKYDIRHTTLQILPAS
                     AGPMEHCNPCNEMAK"
     gene            complement(13670..>14439)
                     /gene="arsB"
                     /locus_tag="PLW16_07645"
     CDS             complement(13670..>14439)
                     /gene="arsB"
                     /locus_tag="PLW16_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPB70816.1"
                     /translation="LAVIFLHNYPEYMVGVILVGLARCIAMVIVWNELAGGDRELAVG
                     LVAFNAIAQVLFYAVYIYFFITLMLNWLGLAEGLDINVSMAESAKTVFIYLGIPFLAG
                     LITRYGLIAVKGEAWFASVFMPKISPLTLVALLFTIVVMFSTQGNKIIAAPWDVVIVA
                     IPMLLYFLIMWFTTFFVIRKIGGNYAQTTAVSFTAASNDFELAIAVAIAIFGIHSGQA
                     FATVIGPLLEVPVLILLVNVALKFKRKYFTAGEFPVR"
BASE COUNT         2759 a         4562 c         4574 g         2544 t
ORIGIN      
        1 ccggcatagc ccagcaggcc gttgacctcg ggatgggacc ggtcgccgat gatcatgatg
       61 tggtactgcc gggcggcgtg cttctggatg atcgcctgta cgtgggcgac cttgggacag
      121 gtggcgtcga tgatgcggat cccgcaggcc ttgatctccc gccgctcccg gggagagata
      181 ccgtgcgccc ggatgatgat cgtcgccgga ccgttcaagg tgtccatgtc gtccaggcgt
      241 tcgattgggg cgatccccct gagcttgagc agctcgatcg tctggggatt atggatcagg
      301 gggccgtagg tatagagctt ttccttgccc cggggcgggg cgaggtccag gaccatatcg
      361 acggcccgct tgacccccat gcagaatccc gcggtcttgg ccagtctaac acccatggga
      421 cgcctcagag acccgttttc gagccagaag aaaaaactcc cggttcccgg ccggccccag
      481 gaggggcgac gggcacgttc cgaccacggc cagcccgaga ccccgcgcgc aggccacgat
      541 gtcctcgatc acgcgggcgt gcaggtccgg atcgcggacc acgccgtgct tgccgacctc
      601 cccgcggctc acctcgaact gcggcttgat cagcgccaga aggctccccc ctccgacgac
      661 gagccgctcc acaaccggca ggaccagctt cagggaaatg aaggagacgt cgatggtcgc
      721 cagatccggc ggcgggtcga ggccttgccc gttaaaatga cggatattaa tacgctccag
      781 gcagacgacg cgagggtccg cgcgcagctt ccaggccagt tgcccgtaac ccacgtccaa
      841 ggcgtacacc cgcgccgccc ccgcctggag cagacagtcg gtgaaaccgc cggtggaggc
      901 ccccacatcg agcgcaacgc acccctgaac gcaaagcggc cagaccgcga gggcccccgc
      961 gagcttcagg ccaccccggc tgacgtacgg ctggtcctgc ccctccagac ggatcgccgc
     1021 cgcggccgga atcatcgcgc ccgccttgtc caccaggtcc tccccgaccc gaacggcgcc
     1081 ggacaggatg agcgcttggg cgcgctcccg ggaggggcac aggccccgct cgacgagcag
     1141 acgatcgagg cggattcttt tttctgccac gcccgacctc atggcgggga agccgcgttc
     1201 atcagccgtc gcgacgccgc gacgattccg tcctcgtcga ggccatatcg ccggcgctgc
     1261 tgggcctggg tggcgtgctc gacgaattca tcggcgatcc ccagccgttg cacccggacg
     1321 ggaccgatgc cgtcacgggc gaaaagttcc aggacggcgc tgccgaagcc gcccatcagg
     1381 acattttctt cgacgaccag gaccttgccg gtgtctgcgg ctgccgtgca cagcagggcc
     1441 tcgtccaggg gcttcacgta gcgggcatcg atcacccggg ccgagacgcc ctccttctgc
     1501 aaccgagccg ccgccgccag ggccggttgc accatcatgc cgacggcgat gatcgcgaga
     1561 tcgccgccct ccgcgacgat ctccccccgg ccgatgtcga ggggccggca cggcatctcg
     1621 aggggcacgc cgatggcctt gccccggggg taacggacgg cggccggacg gccgcaggaa
     1681 acggcggtct ggagcatccg ctggagttcg ttttcatccc ggggcgccat gacgatgaga
     1741 ttgggaatcg accggagata ggagaggtcg aacatgccct ggtgcgttgc accgtcgtcg
     1801 ccgacgagtc ccccccggtc gagggcgaag acgaccggga ggttctgcag gcagacgtca
     1861 tgaatgatct gatcataggc gcgctggaga aacgtcgaat agatggccac cacggggatc
     1921 agcccctccg tcgccaggcc agcggcgaag gtcacggcat gctgctccgc gatgccgacg
     1981 tcgaagaagc ggtggggata ctcccgggca aacccggtga gccccgtccc ctcacacatg
     2041 gccgccgtga tggcgacgat ccggctgtcc tccccggcca gacggacgag cgtccggccg
     2101 aagacttcgg tatacgtcgg ggtgggatct gccaccgcca tggaatggcc ggtttccgcg
     2161 tcgaacgtgg agatcccgtg gaacttcagg ggctccgttt cggcaaaggc ataccccttc
     2221 cccttggtgg tcaagacatg caccagaatg gggccctcca tctcgcgcac gttttgcagg
     2281 gtgcggataa ggcggtccag gcggtggccc tgcaacggcc cgacgtaggt gaatcccagg
     2341 tcctcgaaca gggcgccggg aacgataaag gccttgagcg actcctccag ctgacgggta
     2401 aatttgacca tctgcacgcc gatgccgggg atcgtcttga gaaacccctt gatctccgcc
     2461 ctcagtttcg tcacggcctg gccggtcatc acccggttca gataggccga catggccccc
     2521 acgttgggag agatggacat ctcgttgtcg ttcaggagga tgatcaggtt cttcttccgg
     2581 tcgccggccc agttgaaggc ctcgagggcg atgccggagg tcatggagcc gtcgccgatg
     2641 acggcgatga ccttgcccgg ttgtcggttg aggcattgcg cctcggcgat gccggcggcg
     2701 gcggagatgg aggtgccgct gtgccccaca ttgaatacgt cataggggct ttcctcgcgc
     2761 ctcgggaacc cgctcaagcc gccctgctgc cgcagggtgg cgaagcggtc gcggcggccg
     2821 gtgatgatct tgtgggcgta cgcctggtgc ccgacatccc agaccagctt gtcctggggg
     2881 gtgttgaaga cgtaatggat ggccagcgtc agttccacag ttcccagtga cgaggccaga
     2941 tggccccccg tccgggcgac ggtccggatg atccgctcgc ggatttcctc cgccaggcgg
     3001 ttcaactgct ccgtgtcgag gcgacggatg tcctccggag aatcaatccg gtcgagccat
     3061 gttgcggtct ggatgtcctt ttccttcatg tgtgttccgt tcctgttgat tcaggactgc
     3121 ctctggatga tgtaggttgc gatccaccgc agggggtcgg cgcgttgatc gaagtccttc
     3181 agggacgtaa tggcctgccg cacgagccgg cgggtcttgc gtttggcggc cgccaggccc
     3241 aggagtgcgg gataggtgat tttcccccgc gcagcatcgc tccccgtggt tttccccagg
     3301 cgacgggcgt ccccctccac gttcaggatg tcgtcggcaa tctggaatgc aagccccgcc
     3361 tgttcgccgt aggccgtcag ggccgccacc gcattgtcgg ccgcaccggc caggacggcg
     3421 cccgcgcgga cggaaacaac gatcatggcg ccggtcttgc gggtgtgaat gtagtgcagc
     3481 gtctcccggt ccatggcctt gcccgccgac tggacgtcga cgacctggcc gccgaccatg
     3541 ccccggaagc ccgccgccgc cgcaacgtcg tggatgacgg cgagccgttg ctccggcggc
     3601 agggccgccc gcccttcggg ccgagacagg agatggaagg cctccgtgag cagggcgtcg
     3661 ccggccagga tggcgatgtc ctctccgaag accttgtggc tcgtcggccg cccgcgccgg
     3721 aggtcgtcgt cgtccatggc cggcaaatcg tcatggatca gggaataggt gtggatgaac
     3781 tccagggcgc acgccaccgg cagaaccgtt tccgccttac ccccgaccgc ctccgccgcc
     3841 gccatgcaca gaatgggtcg gagcctcttc ccgccggcaa gcacgctgtg gcgcatggcc
     3901 cggaagatga ccggcggaaa ggcgtcttcg gcgggcagga accgcgccag cgcctcctcg
     3961 acccgggatt gtttttcctt caggtaggct tgcagcttca aggcctcgct gttgtcccgt
     4021 ttcccgtcgt ccacggcgat caatcctcga cgtccgcggc gggcaggggt cgcacggcca
     4081 gatcgtctcc gtcgcggagg agcacctcca cacgccgttc cgcctcgtcg agcttttgcg
     4141 cgcactgccg cgagagacgg atgccctcct caaaggcctt gagggattcc tccagggaaa
     4201 gctcgccgct ttccatcttg cgcacgagcg cctccagctt gtggagggcg tcttcgaatt
     4261 tgtccttggc cattcctcac tgctcctgtt cgatcttcag gacccgggcc gtcaggcggc
     4321 cccgggaaag ttgcacggcc acctcgccgc cggccgcgac ggcgtccgcc cggcggagga
     4381 tcgccccgtc tggaaggcgg cggacgatgc cgtatccacg gttgagcacg ctcagggggc
     4441 tgagagagcc cagcagggcc gtaccggcct tgaggcgttc ccggctgcgc gccagcaggg
     4501 attggaagcc ctgctccagg cggcggcggt aatccccact caggaggcgt ttttcccgaa
     4561 ccgtctgccg gggatccctc tgactcaaac gcgtccggag gcccgaccac gccgcggcct
     4621 gggccgctgc ccgctggcgg agaccgcgcc gcagccgctc gctaaagtcg tcgaccgtca
     4681 gccggtgatc gccgatgacc cgccccgggt ggcggaggcg atccccggcc gctcggaccc
     4741 gttccctaaa cccccgcagg cacagttcct gctgccggat caggcgacgg gcgagcgcat
     4801 ccaccgtccc ctgcagttcg ttccggaccg gcacggccag ttccgccgcc gccgaggggg
     4861 tcggggcgcg gagatcggcc acgaaatcgg caatggtaaa atccgtctcg tgaccgacgg
     4921 cggaaaccac cggcaggcga caccgggcaa tcgcccgcgc cacgccctcg tcattgaaag
     4981 gcgcgaggtc ctccagggaa ccgccccccc gggcgaggat gacacagtcg acgtcggccc
     5041 gggtctggac atcttgaagg gcccggatga tctcggcgga ggcctccgga ccctgcaccc
     5101 gcacgggaac gagcaagatc ggcacggagg ggaaacgccg accggtcacg ttgaggatat
     5161 cccgaatcac ggcgcccgtg ggagaggtga tgacggcgat ccggtggggg aggaacggga
     5221 gcggtcgctt gcgggccgca tcgaagagtc cttcggcctc caggcgtgat ttgagctgtt
     5281 cgaaggcctt ctgcagggcg cccagtccga gcggctccac gtgatccacg atcagctgat
     5341 aatcgccgcg gggcggataa acgctcaccc ggccccggca gacgaccccc attccctcct
     5401 cgagggaaaa ccggttcgcg cgctcggggg cgaaggcgga acggaagagt acgccccgga
     5461 tctgggagcg gtcgtccttc agggtaaagt actggtgccc cgaggcgggc cggcgcaggt
     5521 tggacacctc gccctcaacc cagacgatcc cgaaaccgtc ttccaaaagt tcccggatac
     5581 gctccgttag ggttgtaacc gtaagaattt ccttcataaa tatcacatcc ccggtggagg
     5641 aacctatcag atatccctcc gtctgacaag cgggattttg ccccttgcgc ggccgcgccg
     5701 ccgccgattt ctcattgaca ggaggccgga agatttcgta tggcggggga aacgaagcgc
     5761 aaaggctcga tccggagggg tcggcctgga aagcagggtc aaggatcatg aaaaagaagg
     5821 actatctgaa gcagcccatc cggcatgtcg atatgaaggc catcgacgcc acgtccatca
     5881 tcgacgccat gaaggagatg tccttctcgg cccgggacct cgccgcggcc gcggagatct
     5941 acgaccggat gctggccgac cggaaatgcg ccatcatcct gaccatcgcc ggcagcacga
     6001 gcgccggcgg ctgcatgcag atttacgccg acctcgtccg gcatcacatg gtcgacgccg
     6061 tcgtggctac cggggccagc atcgtggaca tggatttctt cgaggcccta ggattccgcc
     6121 actaccgcgg cacccctcag gtcgacgacc gggagctgcg ccggctttac atcgatcgga
     6181 tctacgacac ctacatcaac gaggaggaat tgcaggcctg cgaccggacg gtcaaggcga
     6241 tcgccgacgc cctgccgccg cgcccctact cgtcccggga attcatccgg gagatgggcc
     6301 gtcacctgac cgtgcacgcc aaaaagaagg attccctggt ccagggcgct tacgaggaag
     6361 acgttccgat cttctgcccc gccttctccg acagcagcgc cgggttcggc ctcgtcctcc
     6421 atcagcacga gcggccggat cagcacgttt ccattgactc cgtcaaggat tttcttgaac
     6481 tgacgaaggt caagatcgcc gccaagacga ccgggctcct catgatcggc ggcggggtgc
     6541 ccaagaactt tgcccaagat acggtgatct gtgcggaggt tctgggcaag gacgtcccca
     6601 tgcacaaata cgccattcag atcaccgtgg ccgacgtgcg tgacggtgcc tgctcgagtt
     6661 ccacgctgaa ggaggccgcg tcgtggggca aggtcgatgt gacccacgag cagatggtgt
     6721 atgccgaagc cacctccgtc ctccccctcc tggcaagtta cgcctatcac cggggaaact
     6781 ggaaaaagcg ccggcactgg gcgttcagca agctgttcgc ttagcgggcg acgcgcagaa
     6841 gcctctcaga gaggctcggc ggtccttttt gagccggtcg cgggatggcg cgcgagggcc
     6901 gaggcgacga tccgctcgat cagcgtctgg taggtgatgc cggccagccc gcagaggatg
     6961 ggaaggtccg aatagccggg ccgcagaccg gccaggggat tgacctcaat gaagttcggg
     7021 ttgccgcgcg catccgaacg gagatcgacc cgccccgcat cccgacagcc caggacccgc
     7081 caggccgcga gcgcaacctc cccggcggcg tccgcctctt tgtcccccgc cagacggtaa
     7141 accacccggt cctcccagtg ttccttgttg gcgaaggaat agacatcccc ttcggcctgc
     7201 gacagccact gcacttccat gaccccgacg acctccgcgg cccgtcccgt gccgatgatc
     7261 ccgacggtga actcgcggcc gggcagaaat tcctcgacca ggaccggctg gcgaaaattt
     7321 ttcagcaggg tccggcagac gcgcgcgagc gcatcgggcg agtttacctt cgacgccccc
     7381 gtcaccccct tgcccgtgcc ttcggcgacg ggtttgacga acaggggaaa agacaggttc
     7441 ggcgcggcga ggtccgcgtg gttttcgatg acaacgaacg ccggcgtggg gagcccgcga
     7501 tcgcggagga tatgcttcgt caagcctttg tggagggtca gggccatcac gagtgcatca
     7561 gagaacgtat aggggatgcc ataggcctcg agcagcgcgg gcacttgggc ctcgcgtccg
     7621 aacccatgca gcccttcggc gatgttgaac accagatccc agcggttgcc gtccaccagt
     7681 cggcgcacca gggatctcag ggggccgatc cggtccaccc cgtggccggc aatttcaagc
     7741 gcgtccgcca gggcccggat cgtttcgggg tggtcgaatt ccgccgtttc ctcgtcgccg
     7801 aacccttggg cgagatagtc ttcccggaga tcgtaggtca tcccgatccg caagccggat
     7861 gccgtcgccg aggccatcag ctgcctccgc ccacaggatg aaaatgggta tcgggacgag
     7921 gattcaatgg atgtcccacc cggaaaccgg atgatccgga tagcggtaaa cctggcccag
     7981 ataattcttg agcaggaggt catctccctg tcggcccagg cagtaacggg gcaggagggg
     8041 caccttgccg ccgccgccgg gggcatcgat cacataggtc ggcacggcgt agcccgtcgt
     8101 atgcccgcgg aggcgttcga tgatctccag ccctttggcc accggcgtcc ggaaatggga
     8161 cgacccgggg atgggatcgc attgatagag atagtaaggc ttcacacgca ggtgaagcag
     8221 gccctggaac atcgttttca tcgtgaccgg gtcgtcgttg attccggaca ggaggacggt
     8281 ctggctgccc agagggatgc cggcattcgc cagccttgcg caggcccggc ccatctccgg
     8341 tgtcagctct tccggatggg tcacgtgaag gctcatccag agggggtgaa acttctttaa
     8401 cacggaaacg agggctgggg tgatccgctg cggcatcacc acgggggcct tggtgccgat
     8461 gcggatcatc tccacgtggg ggatccggtg caactcggcc agcaaccacg ccaggttttc
     8521 gtccgccagg gtcaggggat ctccgccgga caagagcacg tcccggacct cggcatggga
     8581 acggatgtag gtcaagccct cgccccactg atcatgggag aacccgtatt tcgcggtccc
     8641 caccatccgc gaacgcgtgc agtagcggca ataggtggag cacaggccgg ttaccagaaa
     8701 aagcacgcga tccggatagc ggtgcacgat gccgggaacg gggctgtcgc gctcctcctc
     8761 caggggatca tcggcttctc ccgccgttcg cagaaactcg tcattggtgg ggatcacggt
     8821 tcggcgcaag ggctgttgcg ggttgtcggc gtcgagcagg ctggcatagt acggcgtaac
     8881 cgcaacaggc aggagtccgg cgtgacggga gatggcgctc ttttcgtctt cggtcagccg
     8941 cagcacgcgg tcgagaacgt cagcggttcc tatccgattt cggaactgcc aattccagtt
     9001 attccagtcc cccggggtgg cctcgggaaa gaaccgtcgc cggaacagcc gccctgggga
     9061 ctcggccagg tcgttcctgc ctttccgggt gagcggactg ggcggatccg tcactgccct
     9121 tgcatcggtc catgccgggt tcagggccgg ttccgctacg gaaaacgccg ccgtcaaggg
     9181 accgggaggc tcaacctcct cactgaccag cgcttcaatc tccatgccgc ctgcctcctt
     9241 tatcctggcc tttatcccaa ccgccagttc acgatttgac ggtataacgc ttcactcgga
     9301 tggtcatccg gccgtcttca tccgtaccga ccaccccctc cgcctcgatg ggctcctgga
     9361 ggaacttgag cagctctcca cccttgcggt tgcgcgcgat gaaatattcc tcctcacggg
     9421 cagtagcaat ggcagcagca acgacgcgat cacccttcca atccacgggg atgacgatcc
     9481 ccgtgacggt gacaagattt tcgcctttct tgcaggccat aggtctgact cccatttcga
     9541 ttcgtcatgt gtagatccgt cctcagacga ctgaaagaat cgtgccagac aggaaccggc
     9601 gggtccgtcg gtgcatccgt cgggaccggg cccggcggaa gtcgttgcgg ggtcccgact
     9661 ctggcgggag ttccgccgga caaatcgcac cccctccaaa gggggtgagg cccggacgtg
     9721 gacccgatgc cccttagaat cggaattact aattccgatc attcagattc aagaactatc
     9781 tgttccgacc tagagcgtgt ctcgatgctc tcgggggatc acgacgtgcg gccggaaacc
     9841 ggcgcggcag aaaacccctt gacttcgcat aacaacgggg cgttagaatg cagacaaaaa
     9901 agaggatttc tcatgccgga attgccagaa gtcgagaccc tccgacgcca gctgaacccg
     9961 ttgatcgccg gggagaccat ccgggggctg gagatcctgg acgaaaagct gcgggggata
    10021 cgcgatccgg cggggaagac catcttggcc ttgtgccgac ggggcaagta tctggagttc
    10081 catctcgatg acggggcgac gctggtgctg cacctgcgga tgtcggggcg tctccgctgg
    10141 ctgactggac cgggggtatt cccccatacg cgcttcaggt tttcgttcga ttccggtcgg
    10201 ctgatcggca tcgacccgcg ccggtttgcg accctcggga gtctctccga tcgtccggcg
    10261 ccgtccccga tcgcagatcc gtcgcaatcc atccgcgccg ggaaatggat ggacatcgcc
    10321 gccggccggc gcctgcctgt caaatccttc ctattggatc agcgtctcgt cgccggcatc
    10381 ggcaacatct acgcctgtga aattctgcac gcagcgggga ttcatcccgg gaggccgtcg
    10441 ggatcgctga ccaaggggga atggatcggg atcgcggcgg ccgcaaaggc gatcctgcgc
    10501 gacgccgtcg cctgccgggg cacgtccatt tccgactggc gcgacctgtt cggccgaccg
    10561 ggcgtctacc agcatcgcct gaaggtctac ggaaaggctg gaaaggcctg tccccggtgc
    10621 gccgcaacgg tggtccgcat ccgtctgggc ggacggggaa cctacttctg cccgatctgc
    10681 cagacgccgt cgcgctgacg gacagcgcgc gactcctgtc tctcgacctt gacgacgatg
    10741 gcagcatttc gcctaatata agacaagatc tacgagatat ccagcgatca tggccaccag
    10801 aaagacgttc aacacaaaag tgacgatcag cttcttccgg aacatcgagc ccagaatgat
    10861 gagttccgga atgctcgctc ccgctccgcc gacgatgaga gccagaaccg tccccagccc
    10921 catccccttg ccgaccagag cggcgctgat cgggataatg gtttccgcac ggatgtacat
    10981 gggcacgccg atcagcgccg cgatcggaat cgaccaaggg ttgcccggtc ctgccaatcg
    11041 cacgatcaag tcctggggaa agaacccgta gatgaaagcc ccgatcccgg caccgagaag
    11101 caggtaccaa aagacaccct tgaaggtgac gattgcagag cgggacgccc gtttcatctt
    11161 ttcactgacg ggggcccccg ccggcccggt tttgccgtct gtcgaaaaat cgacggaaca
    11221 gcagggcgca gcgctttcct ggactgattc ctgaacggat gaggaacagc agtcgtcgac
    11281 agccgcaaac atcgggagat tcatggatag ggaagaagcc cctaccggag cgtaaaagcc
    11341 gggtccggcg gcatcgattt tcacggtgcc tgtcacgggc tttttcgccg ggctgcagca
    11401 ggtgctctcc agaccgatca ggggtttgac ctgatcttcc atccccatcc tggacatcaa
    11461 ggtcgccacg gccatggaac cgaagaacgt aacgacgaca taaaagacgg tcacgttgac
    11521 gccgaaaagc gagagcaaca aggcgacgat gaacggattg agaaccggcg aggcaaagag
    11581 aaaggccatg accggaccga acggcacgcc ccccttgagc agtccggccg tgatcggcac
    11641 ggtggaacag gaacagaagg gcgtcagggc gccaagtccc gcgcccagca cgtactgcac
    11701 caagcttaac ttgcccgcaa gatagtcccg gatccgcgac ggaggaagat attcggtcag
    11761 gagacctatc aggaaggata cggccacgaa gatcagtatc aattcgccgg caatcaccag
    11821 gaagaatcga ccggcgatga tgaaattgtt caggtcgagc atgaatcgcg tctcctatcc
    11881 cgatattgag gatttcagga tttttcgccc gggcaaggga cactttcttc ctgcacgggg
    11941 ccggaagcgg cgcccccgat tgcccggtag atgtaaggca tggaggagcg ccacgcctcc
    12001 agtgcgactc gatacgcctc cagccccgcc ggggtcaggg tatagacccg ccgtttctgt
    12061 gcgcctttga gctgccggtc ctcgcggacc tccgcatagc cgtgtcgagt cagttccttg
    12121 aggatgggat agatcgaccc cagcgtcggg atgcagcagc cgctggtcag ggatccaagt
    12181 gccttcaaaa ttccgtagcc gtggttaggc ccggcggaca ggacctgcag gaccagaatc
    12241 ttggtaaacc caatattgat cagtgatttc caataattgc tgtccgtcag gtccgttctg
    12301 cctctccgcc gtcacgcctg ttatattgaa ggtcaatata taggtacgtt tcatatgcat
    12361 tcagaattgt caaatctttt ctggatggat ccacagagtg attccctcgc cgtgaagcgg
    12421 ccccgtcgcg gcttgccctg tgccggcaga gcaacgattc cgggtggctg ccacgtcccc
    12481 tgcccatcga ggcgacctgg gggaagcctt gccggatatc gactcgccgg cggccataac
    12541 catgcgcctt gacgaaaggc cgggaaacat gcagcggcat tttaccccaa gcggcagatg
    12601 gcaagccgcc actgaacgga tgtatcgtgc gatccgaggg tcttgcggtg tgtggcggga
    12661 gtctactttg ccatttcatt gcaggggttg cagtgctcca tcggtccggc agaggcgggc
    12721 aggatctgga gcgttgtatg acggatgtcg tacttgcgca tcaaaatctt gtgggccgtg
    12781 cgaatgatgg cttcgtggtc cggtgaatcc tcagcggata cgagatgcgc ggaaagcagt
    12841 ttttgatcgg tcccggtctg ccagatgtgc agatcattga cctccgatac ctcgggaatg
    12901 gccagcaggt cattcctcac ctcggcaaag tcgatgcccg gaggcacgga atcgagcagg
    12961 gagttaaccg tctgacggat cagcttgtag atttcgcggg cgaggatgaa gcagatgacc
    13021 agcgagatga tgggatcgat gaaatgccac ccgaaaaagg tgatcagcaa ggcgcctgca
    13081 atcaccccgg cggaaaacaa ggcgtcctga aatgaatgca gccagacgct cttcaggttg
    13141 atgttccgtg cagacatctt ctggaggagc agcgtcacaa tcgtgttgcc ggcgaaggct
    13201 gcaacgccga cccagagcat tacctggccc gggacttcgg tcggcgtggt gaatcgcttc
    13261 agggtttccc aaaacacaac ggcgatgaca acggacagac cgatgctgtt gacgaaggca
    13321 gcgataaact cgatcttccg atagccgtaa gtttttcggt tattagcggg acggcgagcc
    13381 accttctccg cccagtagct gaagagcatg tgggcgatgt cgctcaggtt gtgtatggca
    13441 tccgagatga gcgccatgct ttggatcagc aggccgaaaa tcatctcgaa agccacgatc
    13501 ccgctgttga tcacaatggc aaaaaaaagg tttctcaacg aagtgtgcgc ttcatcgctg
    13561 tgacggtgct tgcgttccat caaatcaacc cgcccggaag ggcttgccga tcaagtagat
    13621 tcccccaagg caatcgcctc ttcacggtca aaacaccctt cagattgttt tacctcaccg
    13681 ggaattcccc tgcggtgaag tatttgcgct tgaacttcag ggccacgttg acaagcagga
    13741 tcaagacggg cacctccaag agcggcccga tcaccgtggc aaaggcctgg ccggaatgga
    13801 tgccgaagat ggcgatggcc acggcgatgg ccagctcgaa gtcgttggag gccgccgtga
    13861 aggaaacggc cgtcgtctgg gcgtagttcc cgccgatctt ccgaatgacg aagaaggttg
    13921 tgaaccacat aatgaggaaa tacaggagca tgggaatcgc cacgatgacc acgtcccacg
    13981 gtgcggcgat gatcttgttc ccctgcgtgg aaaacatgac cacgattgtg aagagaagcg
    14041 ccaccagcgt cagcgggctg attttcggca tgaagacgct ggcgaaccag gcctccccct
    14101 tgacggcgat caggccgtag cgggtgatga gccccgccag gaaggggatg ccgagataga
    14161 taaagacggt cttcgccgat tccgccatgg acacgttgat gtcgaggcct tccgcgagtc
    14221 caagccagtt gagcatcagc gtgatgaaaa aatagatgta cacggcgtag aagaggacct
    14281 gggcgatggc gttgaaggcg acgagcccca cggccagttc ccggtctccg ccggccagtt
    14341 cgttccagac gatgaccatg gcgatgcagc gggcgagccc gacgaggatg acgccgacca
    14401 tgtattccgg gtagttatgc aggaagatca ccgccagga
//
LOCUS       DAOOLJ010000045        14435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4504_length_14435_cov_16.949861, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000045 DAOOLJ010000000
VERSION     DAOOLJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_14435_cov_16.949861"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLW16_07650"
     CDS             complement(<1..431)
                     /locus_tag="PLW16_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70817.1"
                     /translation="MSTAVWGAIVVLALLLIVVVLLLALRKPQDISVLLGPLSTLTQT
                     LGALQSDLRGLGERIATVERDQGRAGVQMTALESRLAETGSVAQGLAETAASIRSGLG
                     AAQETLASLHSQAKARQHLEERTADSIRRLEAVLAGTHSKGA"
     gene            complement(537..1388)
                     /locus_tag="PLW16_07655"
     CDS             complement(537..1388)
                     /locus_tag="PLW16_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70818.1"
                     /translation="MNCFKRVCRIARTGLLIVAALAVPAAPLFAEAAAPAGEVSVGVL
                     NQYIWRGYESSRHSVVIQPSVTVGYRGLSANLWGNLDTNPYAQPGDDSHAANWNETDL
                     TLSYARTLDRFTLGGGYVYCGLAAVNEDAADRKDSQELFATVSLNVPLTPTLTVYWEF
                     AHYRNWYFLLGVSHVFELGKAVSLKLSAAASYLLSTDADTYPKYDGDGVATDDKFRNF
                     HDGTVSASLPVKVTDRITLTPALSYIFPLTRDARDEMRGLGLKGGPPADRDGDFWVGG
                     ITASFSF"
     gene            complement(1485..1832)
                     /locus_tag="PLW16_07660"
     CDS             complement(1485..1832)
                     /locus_tag="PLW16_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPB70819.1"
                     /translation="MKYIVAIIQPHRLEEVKQALEAVDVNLMTVTNVLGQGRQKGHTE
                     IYRGAKEVGGLLRKIRLDIAVNEAFVEPTIAAISQGAKAEEGQVGDGKIFVLDLADCI
                     RISSGERGGVAIG"
     gene            complement(1845..3089)
                     /locus_tag="PLW16_07665"
     CDS             complement(1845..3089)
                     /locus_tag="PLW16_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPB70820.1"
                     /translation="MIIMNTGDTAWILMSSVLVLGMTIPGLAFFYGGLVRRKNVLSIL
                     MQCFIIVCVISLQWVLFGYSLAFGPDTGCGIIGSLKWAGLHSVGGQPNADYAATLPHQ
                     VFMIFQAMFAIITPALIIGAFAERMKFSAFLIFTLLWSTLVYDPLAHWVWGSGGWLRG
                     LGALDFAGGIVVHVSSGVSALTLAILLGKRCGYDSRPFRPHNLPYTVLGAALLWFGWF
                     GFNAGSALGANELAANAFVTTHVATAAAGFTWALIEWRHHGAPTVLGVVTGAVAGLVA
                     ITPACGFVNAMNAILIGMLVAVFCYVAVVWLKAKLGYDDALDAFGVHGVGGIWGTIAT
                     GLFAEKAINAAGANGLFFGNPRQLLVQLLLVVIAPIFAAVMTWILFKVVDGWIGMRVE
                     KQDEIVGLDLTQQSESAYTVIE"
     gene            complement(3567..4625)
                     /locus_tag="PLW16_07670"
     CDS             complement(3567..4625)
                     /locus_tag="PLW16_07670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB70821.1"
                     /translation="MQKRQINPVPSSFEAFVPEIYLPRIQLFVRLLMGILSAFYFNFL
                     PVPLLILSLTQLNIIIVSYWTFHLLWWRYYRRHGANRTIIRLGSWVDMLGAFTAALCD
                     PFSIPPTILLFLIAVLGNGIQHGLFVIVESMIGALLLGGGALVFHHRLLGDAPPYNLY
                     FYVFLIVVGVYYAYLLVRRIERMKIEAIRIGESDSLTGLRNRRAFLKIAEYLLLLNER
                     SDLALVFVFADLDNFKAVNDTHGHEQGDEVLRRFADTAQSIFRKSDILARYGGDEFVM
                     VLPNTSLEAAESAVRRLQNDFGNWARNSGLQTGVSAGLAIAAKGKNNIEDILSRADAE
                     LYNAKQKMRQGARVLPHP"
     gene            complement(4861..5619)
                     /locus_tag="PLW16_07675"
     CDS             complement(4861..5619)
                     /locus_tag="PLW16_07675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB70822.1"
                     /translation="MQLKGRKAFITGGDRGIGKGIALALAREGADIGLSYRKNREAAE
                     TTVREIETAGRRALAVQADVTEPEQVRAAVETVLGQLGAVDILVSNAGIASRGRFIQE
                     TDPEEMLCLFNIHVMGAFHVTQALLPVLRQQPRSDIVFISSVGSHKCAAGHGPYAAAK
                     AGLDALAMVLAKEELANRIHVNCLACGLVETDMGAKLVRGAMGAELKDISKDLPFGRV
                     CQPADVGHLCAFLCSEKGSYLSGHIIWLDGGIQW"
     gene            5760..6080
                     /gene="sugE"
                     /locus_tag="PLW16_07680"
     CDS             5760..6080
                     /gene="sugE"
                     /locus_tag="PLW16_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HPB70823.1"
                     /translation="MAWIYLLIAGLMETAWAIGLKYTAGFTKLWPSLWTLAGMAISFY
                     LLSLALKTIPVGTGYAVWTGIGAVGTAILGIVLFGESHSAIRIFCIAMIVAGIAGLRL
                     ASPG"
     gene            complement(6162..6965)
                     /locus_tag="PLW16_07685"
     CDS             complement(6162..6965)
                     /locus_tag="PLW16_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB70824.1"
                     /translation="MEAFQTFTLEEIEPQIGLVTLNRPDQLNAINVAMLDDFNALFAK
                     LAKDDAIRVLIITGAGRGFCAGADLNDAIVHKDTTAFADPENFLRLVQERYAALILGL
                     RRIPQPVIAAVNGAAAGGGFSMALACDIRVAAPEASFVASFANIGLSGGELGTSYLLP
                     RLIGVARSSEILYTGRKVKADEAERMGLVNQVVPRETLLEAALSYARPMIAKNVGALK
                     LTKRVLDQNIDAPSLEAAVNLENRNQTLMVFSGEFFKLIQPFFKGGAKG"
     gene            complement(7044..8720)
                     /locus_tag="PLW16_07690"
     CDS             complement(7044..8720)
                     /locus_tag="PLW16_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70825.1"
                     /translation="MAFERVWHKSYAPGVPPEIEIQKVTVGEALIRTAKRFPDRTGFI
                     FMGRRITYRELDTLVNRFANALTGLGLKAGDKVGMLLPNLPQLIIANHAVHRLGAVTA
                     MNNPLYTEQELEHQLNDCDARFLITLDLLLPRALALKSQTSVEAIITCHINDYLPFPK
                     KQLYPFVKKTMYRKVTPQLGVHPFTDLIRKAPATPVPDRSRWDDLGALIYTGGTTGVS
                     KGTMLTNANLSSVVQIFRAWFPDLKDGAVHIVGTYPIFHSAGYVVSQEFIIWSGWTST
                     IIPRPEPGILMETLKKYKPDFLPGVASIFVGLLANPEFRKMDLSFIKGFFTGAAPIAA
                     DTVQDLKKLTGKDVYDNYGMTENIAFATGMPWGGKKKIGTVGVPFPNTDVKIVDLDLG
                     ETELKPGEAGEICIKGPQVMTGYYKRPDETVLALRDGWLYTGDIGVMDEEGHFRIVDR
                     KKDLIVAGGYNIYPKDIDELLFEHERILEACAVGVPDTYRGETVKVFVVLKEGERMTE
                     DEVIAYCREHLAAYKVPKRVEFVTELPKSTVGKILRRELRDREIAKQGKT"
     gene            complement(8765..10429)
                     /locus_tag="PLW16_07695"
     CDS             complement(8765..10429)
                     /locus_tag="PLW16_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70826.1"
                     /translation="MEDAYAAKPWLKHYDKNVPEKLDYPSMAYADALRKAFRDFPERV
                     AVHYMGTAIPYRDLDAFSNQFARFLTAKGCRPGDTVGSHLPNIPASYIGSAGIQKAGC
                     VFTGVSPLLTEEELAYQLNDSGAKVLVTVDILFPVASKAVVKTGVKTVVVASVFDYLP
                     QDIPASPVTPLPGIETISFKEAIGAMPTDPVEVAIDPGAPCLMMYTGGTTGPPKGALL
                     TNNNVVHHMVQMNAWVNLHMAEHIVLSAFPMFHQAGNFIVLWSLGMASTLVAIPNPRD
                     LQYIVKAIETYKPTVIVNVPTIFLELMKLPEFRALDFSGIQFFVSGASAFPAENIREF
                     EEIVGKGKLIEVCGMTETTPIIVALPRDGVKKIGSVGMPVSDTEVKLIDPESRKPVPI
                     GEPGEIVARGPQVFTLGYHNKPEETDNTLRDGWIYTGDVAVMDEDGYFTIVDRLKDMV
                     SVSGFKVFTRVVDDELIEHPDIDNAATIGLPDPKRPGSEIVASAVVLKSGRVGDDAMR
                     EKITAYMKEKVAPYKVPKVIQFMDALPMSAVGKVLKRELRKIMHVE"
     gene            complement(10565..12241)
                     /locus_tag="PLW16_07700"
     CDS             complement(10565..12241)
                     /locus_tag="PLW16_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70827.1"
                     /translation="MSQESIYVQKPWLKHYDPGVPATLEYPQVSYVELIRKAFDQVPD
                     RAALCYMGQQIPYRELDRLSNQMAHVLEKSGCRPGDTVGVHLPNLPACYITAIAIQKA
                     GCVFTGVSPLLTPEELAYQLKDSGAKALVTLDFFYDVVTSVIGGTGVRAVFVTGLNDF
                     LPQAETAAPLAAIPGVTTARFLEALADAPADPVQVRVDPGDPCLMMYTGGTTGQPKGA
                     VLTQNNIVHQIVQLTAWMRSAMGEQTILCAFPLFHQAGNIIALWSLAVGSTLPMVPNP
                     RDLDFIIQSIKDYRPTAIYNVPTLFLELLKRPDFRALDFSGVQYFGSGASPFPAESIK
                     ELEDLVGAGKLIEVYGMTETSPILTALPRDGVKKAGSVGLPLPDTEVRLVDPDTNREA
                     PLGEPGEFVARGPQVFTRGYHNKTEDTAFTLRNGWIHTGDIAVMDEDGYLFIVDRLKD
                     MVSVSGYKVFTRQVDEVLMQHPDIDNVATIGLPDPKRPGSEIVACGVILKSGREKGDA
                     MRENIVAYMKEKVAPYKVPKVIRFVDQLPMSAVGKILKKDMRKVLMEAET"
     gene            complement(12338..13879)
                     /locus_tag="PLW16_07705"
     CDS             complement(12338..13879)
                     /locus_tag="PLW16_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70828.1"
                     /translation="MAGKATGSFNIGNYVKVAAARYGDREAISCMTTGRRLTFRQVNQ
                     RVNGLANGLLGLGIRKGDNTAFLSYNRHEIVETYFALGKIGSVGLPLNYRLSPQEMVE
                     LMSFCDTENLIFDAAFTALVDGLRPKLPGVKRFVSFGEPVPDFAVSYEGLIQSSPQTE
                     PDVEIDEEDPQYLNLTSGTTGLPKAYMLSQYNNAGAGPILTYMHDVTASDVVMTVFPI
                     FGRVGFAWSACSIFAGARNVIHQFDLMKILGVIEQEKVTISNWVPTMASFVLALPDIE
                     KHDLSSLRALVFAASPFPVALQEQVKKRLCPKLYEFYGLQETGILVNIGPKDKERKPE
                     AVGILYFGADVRVVDNNGQDVPAGEVGEIIGRGVAVTAGYFKNEEKTREMFKDGWFYT
                     GDLGRFDDEGFLFLAGRKKDMLITGGQNVFAVEVEEMLMSHPAVMQCAVIGLPDTTWG
                     ERITAVIIRRPGQEVTEEEIIAFSRDRIAHFKAPKTVFFVDNLPMTPTGKVQKFLLVE
                     KFSQS"
BASE COUNT         2613 a         4564 c         4494 g         2764 t
ORIGIN      
        1 gcccccttcg agtgcgtccc ggcgagcacc gcctcgaggc gccggatgga gtcggcggtc
       61 cgttcctcca gatgctggcg cgccttggct tggctgtgca gcgaggccag ggtctcctgg
      121 gccgcgccga gccccgaacg gatggacgcc gccgtttccg cgagtccctg tgccaccgag
      181 cccgtctcgg ccagccgact ctccagcgcc gtcatctgca cgcccgcccg gccctggtca
      241 cgctccacgg tggcgatgcg ctcgccgagc ccccgcagat cggactgcag ggcccccagg
      301 gtctgcgtca gggtcgaaag cggaccgagg agcacggaga tgtcctgcgg tttccggagc
      361 gccagcagca ggaccacgac gatcagcagc aacgccaaga cgacgatggc cccccaaacg
      421 gcagtggaca tgacgggctt tcctccttca atgccggtcc tccgggggcg ccgcagcgga
      481 tcgtccccga caacccgaga ccgcacgatg cgtgcatagc acgttcgggg ctttttttaa
      541 aagctgaaac tcgccgtgat cccgccgacc cagaaatcac cgtcacggtc ggcaggcgga
      601 ccgcccttca ggcccagccc tctcatctcg tcccttgcgt cgcgggtcag gggaaagatg
      661 taggagaggg ccggcgtgag ggtgatgcgg tcggtcacct tgacaggcag gctcgccgaa
      721 acggtcccgt cgtgaaaatt cctgaacttg tcgtcggtcg ccacgccgtc gccgtcgtac
      781 tttggatagg tgtcggcgtc ggtgctcagc agataacttg ccgcggccga cagcttcagg
      841 gacaccgcct tgcccaattc gaacacgtgg gacacgccga gcagaaaata ccagttccgg
      901 tagtgggcga actcccagta gaccgtcagc gtgggcgtca agggcacgtt gagggagacg
      961 gtcgcaaaga gctcctgcga atctttccgg tccgccgcgt cttcgttgac ggcggctaag
     1021 ccgcagtaga cgtaaccgcc gcccagggtg aaccggtcca gcgtcctcgc gtaggaaagg
     1081 gtcaggtccg tttcgttcca gttcgccgcg tgggaatcgt ctccgggttg cgcgtacggg
     1141 ttcgtatcca ggttccccca gaggttggcg gaaaggcccc tgtagccgac ggtcaccgat
     1201 ggctggatga caacgctgtg acggctggac tcgtaccctc gccagatgta ctgattcaga
     1261 acgccgacgg atacctcgcc ggcaggcgcg gccgcttcgg caaaaagcgg cgccgccggg
     1321 acggcgagcg ccgcgacgat gagaagaccc gtccgggcta tcctgcatac tcgtttgaaa
     1381 cagttcatgt gcgcatctcc tttagggttt attccggcgt ttgtcaaggc gcgcggggcg
     1441 gccgtttcgc ccctccgcgc cgccgggctt cggagcgggg cggttcagcc gatggcgacg
     1501 ccgccccgct cgccggaact gatccggatg cagtcggcca ggtcgaggac aaaaatcttg
     1561 ccgtccccga cctggccctc ttcggctttg gccccctggc tgatggcggc gatcgtcggc
     1621 tccacgaagg cctcattcac ggcaatgtcg agccggatct tgcggaggag accgccgacc
     1681 tccttcgctc cccggtagat ttcggtatgt cccttctgcc gcccctggcc gagcacgttg
     1741 gtgacggtca tcaaattgac gtccaccgct tccagggctt gcttgacttc ctccaggcgg
     1801 tgcggctgga tgatcgcgac gatgtatttc atggtctggt cctcctattc gatgacggtg
     1861 taggccgatt cgctctgctg ggtgaggtcg aggccgacga tctcgtcctg cttttccacc
     1921 cgcattccga tccatccgtc cacgaccttg aaaaggatcc aggtcatgac cgcggcaaag
     1981 atcggcgcga tgacgaccag gagcagttgg acgagcagtt gtctggggtt gccgaaaaag
     2041 agcccgttcg ccccggcggc gttgatcgcc ttttcggcga acaggcccgt ggcgatcgtt
     2101 ccccagatgc cgcccacgcc gtggacgccg aatgcgtcga gggcgtcgtc gtacccgagt
     2161 ttcgccttga gccagacgac ggccacgtag cagaagacgg cgaccagcat gccgatgagg
     2221 atcgcgttca tcgcgttgac gaaaccgcag gccggggtga tcgcgacgag tcccgcgacg
     2281 gccccggtca cgaccccgag gaccgtgggc gccccatggt gccgccattc gatgagcgcc
     2341 caggtgaagc ccgccgccgc cgtggcgacg tgcgtcgtca cgaaggcgtt tgcggcgagt
     2401 tcattcgccc ccagggcgct gccggcgttg aagccgaacc agccgaacca gaggagcgcc
     2461 gcccccagaa ccgtgtaggg gaggttgtgc ggccggaacg gccgcgaatc gtacccgcac
     2521 cgtttcccca gcagaatcgc gagcgtcagg gcggaaaccc cggaactcac gtggacgacg
     2581 atgccgccgg cgaaatcgag ggccccgagg ccccggagcc agccgcccga cccccagacc
     2641 cagtgggcga gcggatcgta gaccagggtc gaccacagga gggtgaagat cagaaaggcc
     2701 gaaaatttca tccgctccgc gaacgcgccg atgatgagcg ccggggtgat gatcgcgaac
     2761 atcgcctgaa agatcatgaa gacctggtgc ggaagggtcg ccgcgtaatc ggcgttcggc
     2821 tgcccgccga cggaatggag gccggcccac ttcaggctgc cgatgattcc gcaccccgta
     2881 tcgggcccga aggcgagcga atagccgaac aggacccact gcaggctgat gacgcagacg
     2941 atgatgaaac actgcatcaa aatggacaga acgtttttcc tccgcaccag gccgccgtaa
     3001 aaaaaggcca ggccggggat cgtcatgccc agaacgagga ccgacgacat gagaatccac
     3061 gctgtatctc ccgtattcat gatgatcatg acctcccttg ccgaatgatt tcgggtttac
     3121 cgcccgccgg gccggcgccg gctgcggggt ccccgtttgt cctgccagga gagcaagacc
     3181 ggtgccaagg gagaacacct cgttgtaacc tcttgaattt gaatgcactt atgaagattc
     3241 gcggggtcgc gtccgatagg gtttcctcga gagaccggac aaatttgttc tttttctctg
     3301 ccggattacg gaagcgtggc tttattttag gacaaatttg tggttttccc gttttctcaa
     3361 agcgaacggt gtcaaaaccg cgcttctcga caggggctct gaccgggtgg aactcctgcg
     3421 gaagatgaga ttcacaaggg gttttacggc gacgccacgc gcatcggcat cttccagcca
     3481 cgggtgctga tcgcgcgagg ctcctgacag ccgccatggg gcgcgttctt tggaagagat
     3541 cgggtcgttt caggcaagga ggggactcag gggtgcggga gcacgcgggc gccctggcgc
     3601 atcttctgct ttgcattata caattctgcg tccgcccggc tcaggatatc ctcgatgttg
     3661 tttttcccct tcgccgcgat ggccagaccg gcgctgacac ccgtctgcaa tccactgttc
     3721 cgtgcccagt tcccgaagtc gttctgcaga cgccggaccg ccgattccgc ggcttcgagg
     3781 gaggtgttcg ggaggaccat cacgaattcg tctccgccgt atcgggccag gatatcggac
     3841 ttccggaaga tcgattgcgc cgtgtcggca aagcgccgga gcacctcgtc gccctgttca
     3901 tgtccgtgcg tgtcgttgac ggccttgaaa ttgtcgaggt cggcgaagac gaaaacgagg
     3961 gctaggtcgg agcgctcgtt gaggagcagc aggtattccg cgatcttcag gaacgcccgc
     4021 cggttgcgaa gcccggtgag gctgtcggac tcgccgattc ggatcgcctc gatcttcatc
     4081 cgttcgatcc gccgcacgag aagataggcg tagtacacgc ccaccacgat cagaaagacg
     4141 taaaagtaaa ggttgtaggg aggggcgtcc ccgagaaggc ggtggtgaaa gacgagcgcg
     4201 cctcctccga ggaggagcgc cccgatcatc gactcgacga tcacgaagag cccgtgctgt
     4261 atgccgttcc ccagaacggc gatcagaaac agcaggatcg tgggcgggat gctgaaagga
     4321 tcgcagagtg ctgcggtaaa agcgcccagc atgtccaccc aggagcccag gcggattatg
     4381 gtccgatttg cgccgtgcct gcggtaatac ctccaccaga gaaggtgaaa cgtccagtag
     4441 gatacgataa tgatattgag ctgggtcagc gacaggatga ggagcggaac ggggaggaaa
     4501 ttgaagtaga acgccgacag gattcccatc agtaggcgga cgaacaactg aatccggggc
     4561 aggtagatct cgggaacaaa ggcctcgaag gacgacggaa ctggattgat ctgcctcttc
     4621 tgcacgatgt cccccgagca cgcccccgga agcccccgcg ggaaagccgt cccaaggcgt
     4681 ttcagaatca cccgtaccga tcggtgcgca tctacccact tggcaggtgg tgttaattta
     4741 ccataaaagc gtcccgacgg gtgaaaatat tcgcggccgc cgaaaaggcc cggtgcggat
     4801 gaacacccgc tgcaaaaatc tcccttccgg atcgggggga tatccttctc ggcgcggctc
     4861 tcaccactgg atgccaccgt ccagccagat gatgtgcccg gacagatagc tgcctttttc
     4921 tgagcagagg aaggcgcaga gatggcccac atccgccggc tggcagacac ggccgaaggg
     4981 caagtccttc gagatgtcct tcagctccgc ccccatggcc cccctgacca gtttggcacc
     5041 catgtcggtc tccaccagcc cgcaggccag gcagttgaca tgaatccggt tcgccagctc
     5101 ctccttggcc agcaccatgg ccagcgcatc cagaccggcc ttcgccgcgg cgtacggacc
     5161 gtggccggcg gcgcatttgt gcgatccgac tgaagagatg aaaacgatgt cgctgcgcgg
     5221 ctgctgacgc aggaccggca gcaaggcctg ggtcacgtga aaggcgccca tcacgtggat
     5281 attaaagagg cagagcatct cctcggggtc ggtctcttgg atgaaacgcc cccgcgaggc
     5341 aatccccgcg ttgctgacca ggatatcgac ggcgcccagt tgccccagga cggtttccac
     5401 cgccgcccgg acctgctccg gttcggtcac gtccgcctga acggccaggg cccgccggcc
     5461 cgccgtctcg atttcccgga ctgtggtttc ggcggcctcc cggttcttcc ggtagctcaa
     5521 cccgatatcc gcaccctccc gggccaacgc cagggcgatg ccttttccga tgccgcggtc
     5581 gccacccgta atgaacgcct ttctgccttt aagttgcatg gggaacctcc tcgcgcgtcg
     5641 cgacgggaaa tagtagatga acgtccgcct ccgccacccg cggcggccga cggttgaccg
     5701 tacccttttc ccccggaatc tgataggatg cccgccatct cagacggggg ggaatctcca
     5761 tggcatggat ctatcttctg atcgcgggtc tgatggaaac ggcgtgggcc atcggcctga
     5821 aatacaccgc gggcttcacg aagctatggc caagcctctg gaccctggcg ggcatggcga
     5881 tcagtttcta tctgctctcc ctggccctga agaccattcc ggtcggcacc ggctacgcgg
     5941 tctggaccgg gatcggcgcc gtgggtacgg cgattctggg gatcgtcctg ttcggcgaat
     6001 cccactccgc aatccggatc ttttgcatcg ccatgatcgt ggccggcatc gccggcctcc
     6061 gtctggcatc cccgggatag accgcactgg cggaagagga ggcggaccgc ccggatcgtg
     6121 ggcgctgggc acggtcggag ggcgcgtctg catccccgtc ttcagccttt ggcgccgccc
     6181 ttgaagaacg gctggatcag cttgaagaac tccccggaga agaccatgag ggtctggttt
     6241 cggttctcca ggttgacggc cgcctcgagc gacggtgcgt cgatgttctg gtccaggacc
     6301 cgcttcgtca gcttgagcgc gccgacgttc ttcgctatca tgggccgggc gtaggacagg
     6361 gccgcctcca ggagggtctc ccgcggcacc acctggttga ccagccccat ccgttccgcc
     6421 tcgtcggcct tcaccttccg gcccgtgtag aggatctccg acgaccgggc gacgccgata
     6481 agcctgggca ggagatacga tgtgcccagt tcgccgcccg acaggccgat gttggcgaac
     6541 gaggcgacga acgaggcctc gggcgcggcc acccggatat cgcacgccag cgccatggaa
     6601 aacccgccgc ccgccgccgc gccgttgacg gccgcgatga cgggttgggg aatcctccga
     6661 agccccagaa tcaaggcggc gtaacgctct tggacgagcc ggaggaagtt ctccggatcg
     6721 gcgaaggccg tcgtgtcctt gtggacgatc gcatcgttga ggtccgcgcc ggcgcaaaac
     6781 ccgcgtccgg ccccggtgat gatcaggacc cggatggcgt cgtccttcgc gagtttcgcg
     6841 aacagggcgt tgaaatcatc cagcatcgcc acgttgatgg cgttgagctg atccggccga
     6901 ttcagggtga cgagcccgat ttgcggctca atctcttcca gagtgaatgt ctgaaacgct
     6961 tccatggttt ctccttgacg ccttttcatc ttgaaatgtc ggctggggtc cgggggctgc
     7021 ccagcgcccc cgccattcaa ccgttaggtc ttcccctgtt ttgcgatctc ccgatcccgc
     7081 aattctcgcc gcaggatctt acccacggtg cttttgggca gttctgtgac aaactcgacg
     7141 cgcttcggca ccttgtaagc cgccagatgc tcgcgacagt aggcgatcac ctcgtcttcc
     7201 gtcatccgct cgccctcctt caggacgacg aagaccttga cggtctcccc ccgataggtg
     7261 tcgggcacgc cgacggcgca ggcctcgagg atcctctcgt gctcgaagag gagctcgtcg
     7321 atgtccttcg gatagatgtt gtagccgccg gcaacaatca agtccttctt gcggtccacg
     7381 atccggaaat gcccctcctc gtccatcacg ccgatgtctc ccgtgtagag ccatccgtcc
     7441 cggagagcca agaccgtctc atccggacgc ttgtagtacc ccgtcatcac ctggggtccc
     7501 ttgatgcaga tctcgcccgc ctcaccgggc ttgagttccg tttccccaag gtcgagatcg
     7561 acgatcttga cgtccgtgtt ggggaagggg actccgacgg tgccgatctt cttcttgccg
     7621 ccccagggca tccccgtcgc gaaggcgatg ttttccgtca tgccatagtt gtcgtagaca
     7681 tccttgccgg tcagtttctt gagatcctgg accgtgtcgg cggcgatggg ggccgcaccg
     7741 gtgaaaaagc ccttgatgaa cgacaggtcc atcttgcgga attccggatt ggcgaggagc
     7801 cccacgaaga tggaggcgac gccgggcagg aagtccggct tgtatttttt cagggtttcc
     7861 ataaggatgc cgggctccgg cctcgggatg atcgtcgatg tccagccgct ccagatgatg
     7921 aactcctggc tcacgacgta gccggccgag tggaagatcg gataggtgcc cacaatgtgc
     7981 accgccccgt ccttcaggtc ggggaaccag gcccggaaga tctgcaccac ggacgagagg
     8041 ttcgcgttcg tcagcatggt cccctttgaa acgcccgtcg tcccgcccgt gtagatcagg
     8101 gctcccaagt cgtcccagcg ggagcggtcg ggcaccgggg ttgcaggggc cttgcggatc
     8161 aggtccgtaa acggatggac ccccagctgc ggcgtcacct tgcggtacat cgtcttcttc
     8221 acgaaagggt agagttgctt ctttgggaag gggagataat cgttgatgtg acaggtgatg
     8281 atcgcctcaa cgctcgtctg cgacttcagg gcgagggcac ggggcaggag caggtccagg
     8341 gtgatgagga accgcgcgtc acagtcgttc agctgatgct cgagttcctg ctcggtgtag
     8401 agcgggttgt tcattgcggt gaccgccccg agccggtgga cggcgtgatt ggcgatgatg
     8461 agctggggaa ggttgggcag gagcatcccc accttgtccc ccgccttcaa ccccagcccc
     8521 gtcaatgcgt tcgcgaaccg gttcacgagc gtgtccagtt cacggtacgt gatgcgccgg
     8581 cccatgaaga tgaatccggt gcgatcggga aaccgcttcg cggtccggat cagggcctcc
     8641 cccacggtga ctttttgaat ctcgatctcc ggcggcaccc ccggggcgta cgatttgtgc
     8701 caaacccttt caaaagccat cttctctaaa acctccttgc aacaatcaat attgacctgc
     8761 agcctcattc gacatgcatg atcttccgca gttcgcgctt gagaaccttt cccacggcgc
     8821 tcatgggcag ggcgtccatg aattgaatga ccttcggcac cttgtagggc gccactttct
     8881 ccttcatata ggccgtgatc ttttcgcgca tcgcgtcgtc cccgacgcgg ccggacttca
     8941 gcaccacggc gctcgccacg atctccgacc cgggccgctt cggatccggc aggccgatcg
     9001 tcgcggcatt gtcgatgtcg ggatgctcga tcagttcgtc gtccacgacc cgggtgaaca
     9061 ccttgaagcc ggagacgctg accatgtcct tcagccggtc cacgatggtg aaatacccgt
     9121 cctcatccat gaccgcgacg tcccccgtat agatccaacc gtctcggagg gtgttgtccg
     9181 tctcctcggg cttgttgtgg taacccaggg taaagacctg gggcccccgg gcgacgatct
     9241 cgccgggttc gccgatgggg accggtttgc gtgattccgg atcgatcagc ttcacctccg
     9301 tgtcgctgac gggcatgccg acggacccga ttttcttcac gccgtcccgg ggcagggcga
     9361 cgatgatcgg ggtggtttcc gtcatgccgc agacctcgat cagcttgccc ttcccgacga
     9421 tctcctcgaa ttcccggatg ttttccgcgg ggaaggccga ggcgccgctg acgaagaact
     9481 ggatgccgga aaagtcgagg gcacggaact ccggcagttt catcagttcc aggaaaatgg
     9541 tgggaacatt gacgatcacc gtcggcttgt aggtctcgat ggccttcacg atgtactgga
     9601 gatcgcgggg attgggaatg gccaccagcg tggaagccat ccccaggctc cacagcacga
     9661 tgaaattccc cgcctggtgg aacatgggga aggcggacag gacgatgtgc tcagccatgt
     9721 gcagattcac ccaggcattc atctgcacca tgtggtggac gacattgttg ttcgtgagca
     9781 gggcgccctt cggcggtccc gtcgtgccgc ccgtgtacat catgagacag ggggcgcccg
     9841 ggtcgatggc cacctcgacc ggatcggtcg gcatggcccc gatggcctcc ttgaagctga
     9901 tcgtctcgat cccggggagc ggcgtcaccg gactcgcggg aatatcctgc ggcaggtaat
     9961 cgaagacgga ggccacaacg accgttttca cccccgtttt gacaaccgcc ttactggcca
    10021 cgggaaacag tatgtccacg gtcacgagga ccttcgcgcc ggaatcgttg agctggtagg
    10081 ccagctcctc ctccgtcagg agggggctaa cccccgtgaa gacgcagccc gccttctgga
    10141 tgccggcgga gccgatgtag ctggccggga tgttgggcag gtgggagccg accgtatccc
    10201 ccggccggca tcccttggcc gtcagaaagc gggcgaactg gttggagaac gcatccaggt
    10261 cgcggtaggg gatcgccgtt cccatgtagt ggacggccac ccgttccggg aagtcccgga
    10321 aggccttcct cagggcgtcg gcataggcca tcgaggggta gtcgagtttc tccgggacat
    10381 tcttgtcgta gtgcttcaac cagggcttcg ccgcgtacgc atcttccatc ttgaccctcc
    10441 ttgattcatc aatacgggtt gatctggaat ggttgatcct tccgcccact caactaaggc
    10501 gcgacttaca cctctcgcgt atcgcatcta tggccgccgc ccgtgatcgg ggacgggtta
    10561 tcgctcaggt ttctgcctcc atcagaacct tccgcatgtc ttttttcaga atcttcccga
    10621 cggcgctcat ggggagctgg tccacgaagc ggatcacctt gggcacctta tagggggcga
    10681 ccttctcctt catgtaggcg acgatgttct cccgcatcgc gtcgcccttc tcccgccccg
    10741 acttcagaat caccccgcag gccacgattt cggatcccgg ccgcttcgga tccggcagac
    10801 cgatcgtcgc gacgttgtcg atgtccggat gctgcatgag gacttcgtca acctgccgtg
    10861 tgaagacctt gtatccggaa acgctgacca tgtccttgag gcgatccacg ataaagagat
    10921 acccgtcctc gtccatgacg gcgatgtccc ccgtatggat ccatccgttc cggagcgtga
    10981 aggccgtatc ctcggttttg ttgtgatacc cgcgcgtgaa gacctggggc ccgcgggcga
    11041 caaattctcc gggctcgccg agtggcgcct cccggttcgt atccggatcc accaggcgga
    11101 cctccgtgtc cggcaggggc aagcccaccg atccggcctt cttgacgccg tcccgcggca
    11161 gggccgtaag tatcgggctc gtctccgtca tgccgtagac ctcgatcagc ttgcccgcac
    11221 cgacgagatc ctccagttcc ttaatgctct cggcggggaa gggagatgcg ccgctcccga
    11281 aatactggac gcccgagaag tcgagggcgc ggaaatcggg tcgcttcaga agttcgagaa
    11341 agagggtcgg cacgttgtag atcgcggtcg gccgatagtc cttgatcgac tgaatgatga
    11401 aatcgaggtc ccggggattg ggcaccatcg gcagcgtgct tccgacggcg aggctccaca
    11461 gggcgatgat gttccccgcc tggtggaaca ggggaaaggc gcacaggatc gtctgttcgc
    11521 ccatcgcgct tctcatccag gcggtcagtt gcacgatctg atggacgatg ttgttttggg
    11581 tgagaaccgc gcccttcggc tgaccggtcg tcccccccgt atacatcatg aggcaggggt
    11641 ccccggggtc cacccgcacc tggacgggat ccgccggtgc atccgccaac gcctccagga
    11701 agcgcgccgt cgtcacgccg gggatcgcgg ccaacggggc cgcggtctcg gcctggggga
    11761 gaaaatcgtt gaggccggtc acgaacaccg cccggacgcc ggtcccgccg atcacgctcg
    11821 tgaccacgtc atagaagaag tccagggtga ccagggcctt ggccccggag tccttcagct
    11881 ggtaggccag ttcctccggc gtcagcaggg ggctgacgcc cgtgaacacg caaccggcct
    11941 tctggatggc aatggccgtg atatagcagg cggggaggtt cggcaggtgg acgcccaccg
    12001 tatccccggg acggcagccg ctcttttcga gaacgtgtgc catctggttg gacagacggt
    12061 ccagttcgcg gtacgggatc tgttgcccca tgtagcagag ggccgcccgg tccggcacct
    12121 gatcgaaggc cttgcgaatc agctctacgt aagagacttg agggtattcc agcgtcgccg
    12181 gaacgcccgg atcatagtgt ttcaaccatg gtttttggac gtaaatcgac tcctgactca
    12241 ttcaccttct ccttcatcaa tcaatttcgg atgccgtccc gcatcccggt tgtccctccc
    12301 ggggaccgca ccgcctgcgg tccccgggag aggggcttta agactgcgaa aacttctcca
    12361 ccaggaggaa cttctggacc ttgcccgtcg gggtcatggg gagattatcc acgaagaaca
    12421 ccgtcttcgg ggccttgaag tgcgcgatcc ggtcgcgact gaaggcgatg atctcctcct
    12481 ccgtcacttc ctggccggga cgccggatga tgacggcggt aatccgttcc ccccaggtgg
    12541 tgtcgggcag gccgatgacg gcgcactgca tcaccgccgg gtgactcatg agcatctcct
    12601 ccacctcgac ggcaaagacg ttctggccgc ccgtgatcag catgtccttc ttccggccgg
    12661 cgagaaagag aaatccctcg tcgtcgaacc ggcccaggtc gccggtgtag aaccagccgt
    12721 ccttgaacat ctcccgtgtc ttttcctcgt tcttgaaata gcccgcggtg acggccacgc
    12781 cccggccgat gatctcgccg acctcccccg ccgggacgtc ctgaccgttg ttgtcgacca
    12841 cgcgcacgtc ggccccgaaa tacaggatcc cgacggcctc cggcttgcgt tccttgtcct
    12901 tcggcccgat gttgacgagg atgcccgtct cctgaaggcc gtaaaactcg tagagtttcg
    12961 ggcacaggcg tttcttgacc tgttcctgca aggccacggg aaacggggag gccgcgaaaa
    13021 cgagagcgcg cagcgacgac aggtcgtgct tctcgatgtc gggcagggcg agaacgaagc
    13081 tcgccatcgt gggcacccag ttggagatgg tcaccttttc ctgctcaatg acccccagaa
    13141 tcttcatcag gtcgaactgg tggatgacgt tgcgggcacc cgcgaagatc gagcaggcgg
    13201 accaggcaaa tccgacgcgg ccgaagatgg gaaaaacggt catgacgacg tcgctcgccg
    13261 tcacgtcgtg catgtaggtc aggatcggac ccgcgccggc gttgttgtac tgcgagagca
    13321 tgtaggcctt cggcagcccc gtcgtccccg aggtcaggtt gaggtactgg ggatcctcct
    13381 cgtcgatttc cacgtccggt tccgtctggg gggaggactg gatcaaccct tcataggaga
    13441 cggcgaagtc cggaaccggc tcgccgaaag agacaaaccg cttgacgccc ggaagcttgg
    13501 ggcgaagtcc gtccacgagc gccgtgaagg cggcgtcgaa gatgaggttt tccgtgtcgc
    13561 agaagctcat gagttcgacc atctcctgcg gcgagaggcg gtagttcagc ggcagcccga
    13621 cgctgccgat cttgcccagg gcaaaatagg tttccacgat ttcgtgtcgg ttgtaggaaa
    13681 gaaaggcggt gttgtccccc ttccggatgc cgagccccag aaggccgttc gccaggccgt
    13741 tgacccgctg gttgacctgc cggaacgtca ggcgacgccc ggtcgtcatg cagctgatcg
    13801 cctcccggtc cccgtagcgc gccgcggcga ccttcacgta gttgccgatg ttgaagcttc
    13861 ccgtcgcttt ccctgccatg cgtccctcct ttttgaatgc aggatgtgat ggttcaccgt
    13921 ccggccccca ccggatcggt ctcagccgct tgtggcatgc acaccggcaa acggacggac
    13981 cccgaccgtt ttacccgccg gtgcggacgc cgaatcgtcg gccggccgat ccggtcggat
    14041 cgggggccgc tggaaagaac agctctggaa tgtgaacgaa aatccggatg atctaacgcg
    14101 ttacccagtt tcagcggcgc gatcgggcac cgctgccgat aaagtcgctg ctgacggaaa
    14161 tgacgattca aaacccctgc tcttcgcgct cccgacggtc cggaaccgct cacgggccat
    14221 ccccccgggt ggcatcgccc ggacggtggc cctagcggag tgaacgcgcg gcgtccgggg
    14281 gccggaaggt tcccggacgc ctgcaaaacc gggacgcccg tggtatgggg ctcgatccgt
    14341 ttctgaattg gaatgtcacc cctatagcat gtcccccgct aggacgcaaa ggcctcttcg
    14401 gggatgtcga tcatggagag gtcctcgctc ttgat
//
LOCUS       DAOOLJ010000046        14025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4709_length_14025_cov_17.385254, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000046 DAOOLJ010000000
VERSION     DAOOLJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4709_length_14025_cov_17.385254"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLW16_07710"
     CDS             <1..560
                     /locus_tag="PLW16_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPB70829.1"
                     /translation="RRGFVLSEGAGVLVLAAEDTLTRFGLDARAEVLGVGWTSDAYHF
                     TNPNAKTIVRAIREAIADAGLMPADIEYINTHGTSTQKGDLTEIDCLRTIFGKGLERI
                     PLSSNKSQIGHTLGAAAAIENALTIEGMQRGVILPTINHRPDPRFADIDCVPDHARRQ
                     RYATALANAFGFGGTNCCIVFGGAG"
     gene            557..1039
                     /locus_tag="PLW16_07715"
     CDS             557..1039
                     /locus_tag="PLW16_07715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPB70830.1"
                     /translation="MKPKAFRPESLFGEGPYVRDAATGLVWHRCSHRALYADTDRSTV
                     VYHANYLKYFELGRTSLMRDAAYPYKEIEAGGYVYPVIETGLTFYQPLHYDDPMWIHT
                     RPGELERVRLRFDYIILHAETGAEVCRGFTRHCALNARGVPVAVDPKTGHLWKTFPQP
                     "
     gene            1036..1932
                     /locus_tag="PLW16_07720"
     CDS             1036..1932
                     /locus_tag="PLW16_07720"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HPB70831.1"
                     /translation="MKHSPEIPLVVPVSAYLRDHRADGRCVLPAAEALQILARTLPPV
                     YDAHRQEGAQFSHLLFVDEAAARVEAVHEIEPLTGGGCHSRLTTRRTGRRTACARRIE
                     HVCVYFPAARGTGTETGEAAASARAGAFRASGERGRDRDEEKRHDSAPFVVPVARLYG
                     ELVPFGPAWRNVTGEVRLTPAGAASALSGGRFPEADGPLGSPFPFDAAMHVACAWGQR
                     YRGVVAFPVGFDRRDVLFPTSSNGRYVCRVSPLGDEGATLRFDIRIDDEERRTVEVIR
                     GLRMRDISRGKRIPPAWVREGA"
     gene            1934..2518
                     /locus_tag="PLW16_07725"
     CDS             1934..2518
                     /locus_tag="PLW16_07725"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB70832.1"
                     /translation="MDVAALCLMAYLAASINFAIIVLNLLGKADPRTQFSGNAGTTNV
                     YRQSGLGWAAVVLLLDVGRAVGFAALALWLLPFVWVPWIGFALILGNRFPCFHGFRGG
                     KGVANYLGFTVLIAPWGAALSCLVWVVVHRIVRVPFIASFFMILTLGGATVAASGGQA
                     IPIAGTAATALFVFSNHRRNIAALIEERRGKAGG"
     gene            2619..4091
                     /locus_tag="PLW16_07730"
     CDS             2619..4091
                     /locus_tag="PLW16_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70833.1"
                     /translation="MRIVLIHPAGSNWVPGKKDITATANRMAPLGLLSIAAYLSREGH
                     EVFVHDCLGPRAVPGPAANARLVLEGDPDLVGFSATTSGFLDAWEMAAAIKAARPEVV
                     TVFGGVHVSALEGALLERFDAIDYLCPGEGEATLAELASGADPAGIAGLIRRDGERVV
                     ANPPRAPLADLDDLPFPAYEKLAGFPRGYNLPLFSYVQFPGATMITSRGCPYQCSYCD
                     RSVFKRGYRYNSAAYIYDHLNHLRTRFGVRHVNIYDDLFTANRPRITALCEKLMQNPL
                     GMQFNCAVRVGQTDRELLAMLKAAGALMVSLGIESADPEMLARHKAGVTLEEVRETVA
                     MIRAAGLRAKGLFMMGLPGETEASIRRTSDFVLALGLDDMNMTKFTPFPGAPLWATIR
                     DEGRFDEDWRRMNCLNFVFVPKGIASRERLDQLYNAHVKRFYTDPVWRRRFRSRLWEH
                     RHSLWHMAKHLPDFIAARRHFEPARNGRRADPIQEESTDR"
     gene            4088..5596
                     /locus_tag="PLW16_07735"
     CDS             4088..5596
                     /locus_tag="PLW16_07735"
                     /inference="COORDINATES: protein motif:HMM:NF036848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70834.1"
                     /translation="MSVSKEMNHPILVGVGQITHREKIVGDSVSAVDLAVRAIDACIL
                     DSGRPEIRDRIDTLSVVNMMTEFRDSPLPEICARVGIAPAFREETSIGGNNPQLLVNR
                     AADRIMAGECKVALLAGAEALYRDRKVWQFMNFEKLFARMRGDGGILGDVRYGESPHE
                     ILHGADRASHIYPLFENALRGELGMSLAEHRAYMNGYFLDMVAVAAGNPLAWFNHGKQ
                     MDNITEPTAGNPLFNFPYTKFMNPVLAVNQAAAVLVTDTETARKPHIPRAKWVYLHGG
                     AEAADKWYISERLSYHASPLIRFTVEASLETAGLTMADIDFLDLYSCFPCATIIAARE
                     IGLDIHHLPPLTITGGLSYFGGAGNNYTMHAIAHAVERLRRHPEETGLVTGVGYFLTK
                     YAVGIYSGREPGKAWRRPRRNLVQERIDALEPPPFCLKPDGPATVETYTVLHDRPDGV
                     PWPVIIARLDSGERCLATAPHGSDLADRMEEEEFIGCRGVVTPGDGGPNLFQ"
     gene            5943..6965
                     /locus_tag="PLW16_07740"
     CDS             5943..6965
                     /locus_tag="PLW16_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70835.1"
                     /translation="MEEKEKKNYLASFNGIPGAERNPTNFQRRDDGNHIYKSDDYYEW
                     WYVDCSFDNGYHMVFTFHFMNMFLKPIVPSIQLMIYKPDGTQTARYDLCKPEEVYAGA
                     DWCDVRMGESWLKDLGNGTYEMYIMIKNVGAHLTLKNIVPGWKLGSGFNYRDEENGRV
                     AGWVVPVPYAETEGELYIKDETIAVRGTAYHDHNWGNFPMHEVCARWYWGRLHSGDYC
                     LDYGWVFPRDPSGPMFAPLLIAKGREIVLSTNIMHTELSDLDKEPKTGQEYAKHLVLT
                     TDNLGVKMTLDIATKRLVELMQLPKTTAWDQWYYRFLADFTMDIEIDGAKDKVAGEML
                     HEYMVL"
     gene            7137..9074
                     /locus_tag="PLW16_07745"
     CDS             7137..9074
                     /locus_tag="PLW16_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70836.1"
                     /translation="MTFDRLFSPFTVAGMALRNRVVMLPMSTGFIEPDETAGERFIRF
                     YEARAEGGAGLILIPFTPMRDGGHVEPGLYDDRFIPSLRPLTDRLHAAGAVAAAQLIL
                     SYFVILKGDTPELVGPSPVVNRLLRAAPRELTTGEIQLIVGEFGRAARRARDGGFDAV
                     EVLVAAGYILNRFLSPISNTRTDAYGGSLENRMRIILEAIDAIRAEAGRDFPVICRLN
                     VEEQMPGGHTIADMKPVLAALERHGVALINTYTGWHESPVPTVAPSLPKGAFAHLSER
                     IKASCNLPVIASNRINDPATAEAILAGGQADLIGMGRALIADPALPNKAREGREAEIT
                     PCLACSQCLAQIMSALYQTRPGAGSIRSLCSVNPLAGREWEPPPAAPARKRRVFVVGA
                     GPGGMTAAATAAARGHDVTVFDAAGAPGGRLVAAALPPFKEEIRTLIESQHVRARQAG
                     VRFRFDHPVDPAFVERGKPDALVLATGADPLCPPIPGLDGPNVIPAEDVLTGRRAVRG
                     RTVVIGGGSVGCETAEFLLERGVKDVTILEMLGRMADNVVPTYRPFFLGRLKAGEVKF
                     STRTTVTGITGGGVNVEREGVAEVVPADTVILAAGYRADPRKIEAFRRAAPEVHAVGD
                     CVRARGIQEAVEEGFAVGAAL"
     gene            9205..10422
                     /locus_tag="PLW16_07750"
     CDS             9205..10422
                     /locus_tag="PLW16_07750"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB70837.1"
                     /translation="MGIDPGDYVHYRDLLKGERLPAAFVDLDRFDANVAYVASTQRAT
                     GKRIRVASKSLRCTALIRRIFARGGASYRGILGFTVEEAAFLAERGFDDLLVAYPTVQ
                     PSDLSLLSRLTASGKQVSLVVDAPAHLERLSAAGERAGVTLSACLEVDLSYRPFRTSL
                     HLGARRSPIRTPAEALALAHAARSLPGVRIDAVMGYEGHIASTNDDLPGARLKNRVIR
                     LMKRASVPELTQRRAAVVRALRNAGVSLRVVNGGGSGSLVSSGRDPSLTEVTAGSAFY
                     GPALFHHFHDVHFQPAAFFAVQVVRKPADGMVTCLGGGYAASGAAGPDKLPRPVLPPG
                     MRLLPLEGAGEVQTPLTLPADGPELNVGDPVIFQHAKAGELAERFNALLLIRGDRIVE
                     RVPTYRGEGVAFL"
     gene            10441..11676
                     /locus_tag="PLW16_07755"
     CDS             10441..11676
                     /locus_tag="PLW16_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70838.1"
                     /translation="MVQRRGVFYGWWIVVGGFVLNFIGIGIGINAIGVFFKPVVDSLG
                     FSRGDFSLYFTIAALSMTAAAPLVGKLLETLNVRVVMGVSTALLAVSFALYSQCQTLT
                     HFYLLSVLVGVGHAGSHIIPVSTLISNWFRRQRGLAMGIVFTATGIGGLVFNPVANGL
                     ILHYGWRETYILLGALIGVICMPTALLLMKRSPEEEGWHPDGAGRAEGAAPEVAAGGY
                     RLGEFAKTPVFWLLAGMILLVNTLNMGIQQHLIPYLTDIGHSSTVAANIMGLYLGMTV
                     FGKLVLGSFCDRRGLKAGLAIFTAILVVGILLLFGAASLPWAVLFGVVYGFGNAIQTV
                     LPPLMTVAAAGLAHFALIYGIMTIFQTLGSGIGMPLSGYLYDWTGGYEAAFYVYIGLA
                     VLAALLGMAALKRARFKAV"
     gene            12125..13039
                     /locus_tag="PLW16_07760"
     CDS             12125..13039
                     /locus_tag="PLW16_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HPB70839.1"
                     /translation="MATSRKFTVALIQMAMSADPGENLRRAVTQIAAAAKRKAGVVCL
                     PELFRSRYFCQREETAAFDLAETIPGPTTEALAGLARRHRVVVIAPLFERRAPGVYHN
                     SVLVIDADGRPAGLYRKMHIPEDPAYYEKFYFTPGDLGFPAFDTRCGRIATLICWDQW
                     FPEAARLAALHGAAAVFYPTAIGWHPREKARFGAAQREAWKTVQRGHAIANGVYVAAV
                     NRVGLERPEVGGDGIEFWGSSFIADPQGIVLAEASPDREEILLAEVDLTHLEDVRRNW
                     PFLRDRRIDAYGGLMERFLDHRVPEKKK"
     gene            13065..>14025
                     /locus_tag="PLW16_07765"
     CDS             13065..>14025
                     /locus_tag="PLW16_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPB70840.1"
                     /translation="MTADRARTPAAEGFRLPAEWEPHAATWLGWPANRTDWPGKFAPI
                     PWVYGEIVRKLAPGEICRIIAQGRIERRARGLLARAGVDPARVEFFDFPSDRGWTRDS
                     GPVFLKREGPPAEVAVAGFRFNAWAKYPDWARDAKIPARAAERLGLRLLPARIGDTDF
                     VLEGGSIDVNGAGALLTTEECLLDPAVQVRNPGFGRAEIERALQDHLGVTRILWLGRG
                     IAGDDTHGHVDDVCRFVNDRMVVLCREADPSDANYRPLEENRERLQGVRLATGASLEV
                     VSLPMPAPLFLDGRRLPASYANFYIANAAVLVPTFNDPNDRIAL"
BASE COUNT         2255 a         4757 c         4672 g         2341 t
ORIGIN      
        1 accggcgcgg cttcgtcctg tccgaaggcg cgggcgtgct cgtgctcgcg gccgaggaca
       61 cgctcacccg gttcggcctg gacgcccggg cggaggtcct gggcgtcggc tggacctccg
      121 atgcgtatca cttcaccaac cccaacgcca aaacgatcgt gcgggccatc cgcgaggcca
      181 tcgccgacgc ggggctgatg ccggcggata tcgagtacat caacacgcac gggacctcca
      241 cgcagaaggg cgacctgacg gagatcgact gcctccggac gatcttcggg aagggcctcg
      301 aacggatccc cctgtcctcg aacaaatcgc agatcggcca cacccttggg gccgcggcgg
      361 ccattgaaaa cgccctgacc atcgaaggca tgcagcgggg ggtaatcctg ccgacgatca
      421 accaccgccc cgatccgcgc ttcgccgata tcgactgcgt ccccgatcac gcccgccgtc
      481 agcgttacgc gaccgccctg gcgaacgcct tcggcttcgg ggggacgaac tgctgcatcg
      541 tcttcggagg ggcgggatga aaccgaaagc gttccggccg gaatccctgt tcggtgaagg
      601 accgtacgtc cgcgatgcgg cgaccggcct cgtctggcac cgctgttccc accgggccct
      661 ctacgccgac acggaccgct ccacggtcgt ctaccacgca aattacctga agtatttcga
      721 gctcgggcgg acatcgctca tgcgcgacgc cgcctatccc tataaggaga tcgaggccgg
      781 gggctatgtc taccccgtca tcgaaaccgg gcttacgttc taccagccgc tccactacga
      841 cgaccccatg tggatccata cccgccccgg cgaactggaa cgggtgcgcc tccgcttcga
      901 ctatatcatt ttgcatgcag agacgggcgc cgaggtctgc cgcgggttca cccgccactg
      961 cgccctgaat gcgaggggcg tgcccgtagc ggtcgacccc aaaacgggtc atctatggaa
     1021 aacctttccc cagccgtgaa acattctccg gaaatccccc ttgtcgttcc cgtttccgcg
     1081 tatctgcgcg atcaccgtgc ggacggacgc tgcgtcctgc ccgccgcgga ggccctgcag
     1141 atcctggccc ggacgctgcc gcccgtctac gacgcccacc gtcaggaggg ggcgcagttc
     1201 tcgcacctcc tcttcgtgga cgaggcggcg gcgcgcgtcg aggcggtcca cgagatcgag
     1261 ccgctgaccg gcgggggctg ccattcccgt ctgacgacgc gccggaccgg ccggcggacg
     1321 gcctgcgccc gccgcatcga gcatgtgtgt gtctattttc ccgccgcgcg aggcaccggg
     1381 accgagaccg gtgaggcagc ggcctccgcc cgggcggggg cgtttcgtgc ctccggggaa
     1441 cgggggcggg atcgcgatga ggagaagcgg catgactcgg cgccctttgt tgttcccgtc
     1501 gcccgcctct acggcgaact ggttccgttc gggcccgcct ggcggaacgt cacgggcgag
     1561 gtgcgtctca cgcccgccgg ggcggcgtca gccctttccg gcggacgttt tccggaggcc
     1621 gacggccccc tggggtcgcc ctttcccttc gacgccgcca tgcacgtggc ctgcgcctgg
     1681 ggacaacgtt accggggcgt cgtggccttt cccgtcgggt tcgaccgccg ggacgttctc
     1741 ttcccgacgt cctccaacgg gcgctacgtc tgtcgggttt ccccccttgg ggacgaaggg
     1801 gcgaccctgc gcttcgatat ccggatcgac gatgaggaac gacggaccgt ggaggtgatc
     1861 cggggcttaa ggatgcggga catctctcgc ggaaagcgaa tcccgccggc ctgggtgagg
     1921 gaaggggcgt agaatggatg ttgccgcgct gtgtctcatg gcgtatctcg ccgcctcgat
     1981 caatttcgcg atcatcgtcc tcaatctcct ggggaaggcg gatccccgaa cccaattcag
     2041 cgggaacgcc ggcacgacga acgtctaccg tcagagcggt ctcggctggg cggccgtcgt
     2101 cctcctgctc gacgtcggcc gggccgtcgg tttcgcggcc ctggccctgt ggctgctgcc
     2161 cttcgtctgg gttccctgga tcggtttcgc cctcatcctc ggaaaccggt ttccctgttt
     2221 tcacggcttc cgcggcggga agggcgtcgc caactatctg ggcttcacgg tcctcatcgc
     2281 cccgtggggg gcggccctgt cctgcctcgt ctgggtcgtc gtccaccgga tcgtccgggt
     2341 cccgtttatc gcctccttct tcatgatcct gaccctcggg ggagcgacgg tcgcggcgag
     2401 cggcggccag gcgattccga tcgccggtac ggcggcgacg gcgctcttcg ttttctcgaa
     2461 ccaccggagg aacatcgcgg cgctgatcga agagagacgg gggaaggccg ggggatgagg
     2521 gccgcggcaa agggggagcg gatcggaaat gacccgccgc gcggaagggt cccgatgtcc
     2581 acgagatgca ggtttacagg cggggaggaa ggggattgat gcgtatcgtt ctgatccacc
     2641 cggcgggctc caactgggtg ccggggaaaa aggacatcac cgcgacggcg aaccggatgg
     2701 ccccgctggg gctcctttcg atcgcggcgt acctgtcccg cgaaggccac gaggtcttcg
     2761 tccacgactg cctgggtccg cgcgccgttc ccgggccggc ggcgaacgcg cgcctcgttc
     2821 tcgagggcga cccggacctc gtcggctttt ccgccacgac ctccgggttt ctcgacgcct
     2881 gggagatggc ggcggcgatc aaggccgccc gtcccgaggt cgtcaccgtc ttcggcggcg
     2941 tgcacgtctc cgccctcgag ggggcgctct tggagcgctt tgacgccatc gattatctct
     3001 gtccggggga gggcgaggcg accctcgcgg aactcgcctc gggcgccgat ccggccggca
     3061 tcgcggggct gatccggcgg gacggggagc gcgtcgtcgc gaatccgccc cgggcgccgc
     3121 tcgcggatct ggacgacctt ccgttcccgg cctacgagaa actcgccggc ttcccgcggg
     3181 gctacaacct gccgctcttc agctacgtcc aatttcccgg cgccacgatg atcaccagcc
     3241 ggggctgccc ctaccagtgc tcctactgcg accgctccgt cttcaagcgc ggctatcgct
     3301 acaactcggc ggcctacatc tacgaccacc tcaatcacct gcgcacgcgc ttcggggtgc
     3361 gccacgtcaa catctacgac gacctcttca ccgccaaccg tccccgcatc acggcgctgt
     3421 gcgaaaaact catgcaaaat ccgctgggga tgcagttcaa ctgcgccgtc cgcgtcggcc
     3481 agacggaccg ggaactcttg gcgatgctaa aggcggcggg ggccctgatg gtctcgctcg
     3541 ggatcgagtc cgccgacccc gagatgctgg cccgccacaa ggcgggcgtg accctggagg
     3601 aggtccgcga gaccgtggcg atgatccggg cagcggggct gagggccaag gggctcttca
     3661 tgatgggtct gccgggcgag acggaggcct ccatccgacg gacgagcgat ttcgtccttg
     3721 ccctgggcct ggacgacatg aacatgacga agttcacccc gtttcccggc gcgccgctct
     3781 gggcgacgat ccgggacgaa gggcggttcg acgaggactg gcggcggatg aactgcctga
     3841 acttcgtctt cgtgccgaag ggcatcgcct cccgcgagcg cctcgaccag ctctacaacg
     3901 cgcacgtcaa gcgcttctac accgaccccg tctggcgccg ccgtttccga agccgcctct
     3961 gggaacaccg acacagcctc tggcacatgg cgaaacacct gcccgatttc atcgccgccc
     4021 ggcgccattt cgagcctgcc cgaaacggga ggcgcgccga tcccattcag gaggaatcca
     4081 cagacagatg agtgtatcca aggaaatgaa ccatcccatt cttgtcggcg tgggacagat
     4141 cacgcaccgc gaaaagatcg tcggggacag cgtttccgcc gtcgatctgg ccgtgcgggc
     4201 gatcgacgcc tgcatcctgg actcggggcg gccggagatc cgcgaccgta tcgacacgct
     4261 ttccgtcgtc aacatgatga cggagttccg ggacagtccc ctccccgaaa tctgcgcccg
     4321 ggtggggatc gcgcccgcct tccgcgaaga aacgtccatt gggggaaaca atccccaact
     4381 gctcgtgaac cgggcggcgg accggatcat ggccggcgag tgcaaggtgg cgctcctggc
     4441 gggcgcagag gccctctacc gggaccggaa ggtctggcag ttcatgaact tcgagaaact
     4501 cttcgcgcgc atgcgtggcg atggcgggat cctgggggac gtgcgctacg gtgaatcccc
     4561 ccacgaaatt ctgcacgggg ccgaccgcgc ctcccatatc tatccgcttt tcgaaaacgc
     4621 cctccgcggg gaactgggca tgagcctggc ggagcaccgg gcgtacatga acggttactt
     4681 cctggacatg gtggcggttg cggccggcaa ccccctggcc tggttcaacc acggcaagca
     4741 gatggacaac atcacggaac ccaccgccgg gaatcccctc ttcaattttc cctacacgaa
     4801 gttcatgaat ccggtgctgg ccgtgaacca ggccgcggcc gtgctggtca ccgatacgga
     4861 aaccgcgcgc aaacctcaca tcccccgtgc aaaatgggtg tatctccacg gcggcgcgga
     4921 ggccgcggac aagtggtaca tctccgaacg gctcagctac cacgcctcgc cgctcatccg
     4981 tttcaccgtc gaggcctccc tggagaccgc cggcctgacg atggcggaca tcgattttct
     5041 ggacctctac agctgttttc cgtgcgccac gatcattgcc gcccgagaga tcggcctgga
     5101 cattcaccat ctgccccccc tgacgatcac cggcggcctg tcctacttcg gcggggccgg
     5161 gaacaactat acgatgcacg ccatcgcgca tgccgtcgag cggctgcgcc ggcatcctga
     5221 ggagacggga ctcgtgacgg gggtgggcta ttttctgacg aaatacgccg tggggatcta
     5281 cagcgggcgc gaacccggca aggcgtggag acgtccgcgg cggaacctcg ttcaggaacg
     5341 gatcgacgcc ctggaacccc cgccgttctg cctcaaaccg gatggacccg ccaccgtgga
     5401 aacctatacg gtcctccacg accggcccga cggggtcccc tggccggtca tcatcgcccg
     5461 gctggattcc ggggaacgct gcctggcaac cgcgccccat ggctccgatc tggcggaccg
     5521 catggaagag gaagagttta tcggttgccg gggcgtggtc acgccggggg acgggggacc
     5581 caacctcttc cagtgaccgt tttcgggacc gccgcgccgc ccgtaacccc tctagaaggc
     5641 acgacaatcc aggaccggaa ggcgactgat cggtcgatca tcggaatttc gtcgtcggaa
     5701 gctccttcgc gccgaacctt caacccgccg gaatgactgt atatataacg agctccctca
     5761 aatatctgtt tgaaggtcga cccgacaatg atatagaacg gccagatcgt cggtccccat
     5821 tcccgccgga gacgcggacc atcgcccggg cgggattcac ccgggcggca gggcggcatc
     5881 cacgaccccg agatgccctt cggcccgaaa gacgatcaca aacaaatcga taggaggcgt
     5941 tcatggaaga gaaagaaaag aaaaactatc tggcgtcgtt caacggcatt cccggtgcgg
     6001 agcgcaatcc caccaatttc cagcgccgcg atgacgggaa ccacatctac aagagcgacg
     6061 attattacga gtggtggtac gtcgactgct ccttcgacaa cggttatcac atggtgttca
     6121 ccttccactt catgaacatg tttttaaagc ccattgttcc ctccatccag ctcatgatct
     6181 acaagccgga cggaacccag acggcgcgct acgacctgtg caagccggag gaggtctatg
     6241 ccggggcgga ctggtgcgac gtccgcatgg gcgagagctg gctcaaggac ctggggaacg
     6301 gcacctacga gatgtacatt atgatcaaaa acgttggggc ccacctgacc ctgaagaaca
     6361 tcgtccccgg ctggaagctg gggtcggggt tcaactaccg tgacgaggaa aacggccggg
     6421 tcgcgggctg ggtggtcccg gtcccgtacg cggagacgga gggcgaactc tacatcaagg
     6481 acgagaccat cgccgttcgc ggcaccgcct accacgacca caactggggg aacttcccga
     6541 tgcacgaggt gtgcgcccgg tggtactggg gccggctcca cagtggcgac tactgcctgg
     6601 actacggctg ggtcttcccg cgggacccga gcggacccat gttcgccccg cttctgatcg
     6661 cgaaggggcg cgagatcgtc ctgtcgacga acatcatgca cacggaactg agcgacctcg
     6721 acaaggagcc caagaccggt caggagtacg cgaagcacct cgtgctcacc acggacaacc
     6781 tcggcgtgaa aatgacactc gacatcgcga cgaagcgcct cgtggaattg atgcagcttc
     6841 ccaagaccac ggcctgggat cagtggtact accggttcct cgcggacttc acgatggaca
     6901 tcgagatcga cggcgcgaag gacaaggtcg ccggcgagat gctccacgag tacatggttc
     6961 tttaaaagcg gcaaacctct tcgggaatcg ttgtcccgcg gcgcgccgga taggtttcga
     7021 ccccgaaaca ttgcctgtgc gcgcccgcgg gggcgtcgtc ccgggcggaa aaacgggccg
     7081 gaacgaccca tcggtcgttc cggtcgccgc ggcaggcggc ttctaaggag cagggcatga
     7141 ccttcgatcg actgtttagc cccttcacgg tggcgggcat ggcattgcgg aaccgggtgg
     7201 tgatgctccc catgagcacg gggttcatcg agcccgacga gacggccggc gaacggttca
     7261 tccgcttcta cgaggcgcgg gccgagggcg gggcggggct gatcctcatc cccttcaccc
     7321 cgatgcggga cggcggacac gtcgagccgg gcctgtacga cgaccgcttc atcccgtcct
     7381 tgcgcccgct gacggaccgg ctccacgccg cgggcgcggt ggcggccgcg cagctgatcc
     7441 tctcgtattt cgtgatcctg aagggcgaca ccccggaact cgtcggcccc tcgccggtcg
     7501 tcaaccgcct gttacgcgcg gccccgcgag aactgacgac cggcgagatc caactgatcg
     7561 tcggcgagtt cggccgtgcc gcccgccggg cgcgcgacgg tggattcgac gcggtcgagg
     7621 ttctcgtcgc ggcgggctat atcctgaacc gcttcctctc cccgatcagc aacacgcgca
     7681 cggacgccta cggcggaagc ctcgagaacc ggatgcggat catcctcgag gcgatcgacg
     7741 ccatccgcgc ggaggccggc cgggatttcc ccgtcatctg ccggctcaac gtggaggagc
     7801 agatgccggg cggtcacacg atcgcggaca tgaaaccggt gctcgctgcc ctcgagcggc
     7861 acggcgtcgc cctgatcaac acctacaccg gctggcacga gtcccccgtc cccacggtgg
     7921 cgccgtcgct gcccaaaggg gccttcgccc acctgtcgga gcggatcaag gcatcctgca
     7981 acctgccggt catcgcctcg aaccggatta acgacccggc gacggcggag gccatcctgg
     8041 ccgggggcca ggccgatctc atcggcatgg gccgggcgct gatcgccgac ccggcgctac
     8101 cgaacaaggc ccgcgagggg cgggaggcgg aaatcacccc gtgcctggcc tgctcccagt
     8161 gcctggcgca gatcatgtcg gcgctctacc aaacccggcc gggcgcggga tccatccggt
     8221 cgctgtgcag cgtcaatccg ctggcggggc gggaatggga gcccccgccc gccgcccccg
     8281 cccggaagag gcgggtcttc gtcgtcggcg cgggtcccgg cgggatgacg gcggccgcga
     8341 ccgccgcggc ccggggccat gacgtcacgg tgttcgatgc ggccggcgcg ccgggcgggc
     8401 ggctggtcgc cgcggcccta ccgccgttca aggaggagat ccggactctc atcgagagcc
     8461 agcatgtgcg ggcgcggcag gccggtgtcc gtttccggtt cgaccacccc gtcgatccgg
     8521 cgttcgtcga gcggggaaaa ccggacgccc tcgtcctggc gacgggcgcc gatccgcttt
     8581 gtccgcccat cccgggtctc gacggtccga atgtgatccc ggcggaggac gtcctgacgg
     8641 gccggcgcgc cgtgcgcggc cgcacggtcg tgatcggcgg cgggtccgtg ggctgcgaga
     8701 cggcggaatt cctcctggag cgcggcgtca aggacgtcac gatcctggag atgctgggac
     8761 gcatggcgga caatgtggtt cccacctacc ggcccttctt cctcggccgc ctgaaggcgg
     8821 gcgaggtgaa gttctcgacc cggaccaccg tcaccgggat caccggcggc ggcgtgaacg
     8881 tggagcggga aggggtcgcg gaggtcgtcc cggcggacac cgtcatcctg gcggcgggct
     8941 acagggcgga tccgcggaag atcgaggcct tccgccgggc cgcgccagag gtccatgccg
     9001 tgggcgactg cgtccgtgcc cgagggatcc aggaggccgt cgaggagggc ttcgccgtcg
     9061 gcgccgccct gtagggctag ggccgccgca ctcccccgga ggccgttcgt ggcggcaagg
     9121 gcggaggggg acggaagacg cgggcgatgc ggtgcccggc gtttctgcat gcagccggaa
     9181 cccggccgcg gaaggggggg tggaatggga atcgatcccg gcgactatgt ccactaccgc
     9241 gacctcctca agggggaacg cctgcccgcg gccttcgtcg acctggaccg gttcgacgcc
     9301 aacgtggcct acgtcgcctc cacccagcgg gccaccggca agcgcatccg cgtcgcctcg
     9361 aagtccctgc gctgtacggc cctgatccgg cgcatcttcg cccggggcgg ggcgtcctac
     9421 cgggggatcc tcggcttcac cgtggaggag gccgcctttc tcgcggagcg gggattcgac
     9481 gacctcctcg tggcctaccc caccgtgcag ccgtcggacc tctctctcct ctcccgcctc
     9541 accgcatccg gaaagcaggt ttcgctcgtg gtggatgcgc ccgcgcacct ggagcgcctg
     9601 tccgccgcgg gcgagcgggc gggtgtcacg ctgagcgcgt gcctcgaagt ggacctgtcg
     9661 taccgccctt tccggacctc cctccacctg ggcgcccggc gcagcccgat ccggacgccg
     9721 gcggaggccc tcgcgctcgc gcacgccgcc cgttcgctgc cgggggtgcg gattgacgcc
     9781 gtcatgggct acgaggggca catcgcctcc acgaacgatg acctgccggg ggcgcggctt
     9841 aagaaccgcg tgatccgcct gatgaagcgg gcgtcggttc cggaactgac gcaaagacga
     9901 gcggccgtgg tccgggccct gaggaatgcc ggcgtgtccc tgcgggtcgt caatggcggg
     9961 ggaagcggca gcctggtctc gtccgggcgc gacccctccc tgacggaagt cacggccggt
    10021 tcggcgtttt acggtccggc cctgttccac cacttccacg acgtccattt ccagcccgcg
    10081 gccttttttg ccgttcaggt cgtgcggaag ccggccgacg gcatggtcac ctgcctggga
    10141 ggggggtatg cggcctccgg ggccgccggt ccggacaagc tcccgcggcc ggtcctgccg
    10201 cccgggatgc gcctgctgcc gctggaaggc gccggcgagg tgcagacgcc gctcaccctt
    10261 ccggccgacg gcccggaact gaacgtcggg gatcccgtca tcttccagca cgccaaggcc
    10321 ggcgaactgg ccgaacgatt caacgcgctc ttgctgatcc gcggcgaccg catcgtggag
    10381 cgggtgccga cgtaccgggg cgagggcgtc gcatttctct gaacgggggg aggggacatc
    10441 atggtgcagc ggcgcggggt cttctacggg tggtggatcg tggtcggcgg cttcgtcctg
    10501 aacttcatcg ggatcgggat cgggatcaac gccatcgggg tcttcttcaa gccggtcgtc
    10561 gactccctcg gcttcagccg gggggacttc tcactgtact tcacgatcgc ggccctgtcc
    10621 atgacggccg cggccccgct cgtgggaaag ctcctggaga cgctgaacgt ccgcgtcgtc
    10681 atgggggtct ccacggcgct cctggccgtc tccttcgccc tctattccca gtgccagacg
    10741 ctcacccatt tctacctgct gtccgtcctg gtcggcgtgg gccatgcggg gagtcacatc
    10801 atccccgtct ccaccctgat cagcaactgg ttcaggcgtc agcgcggtct tgcgatgggg
    10861 atcgttttca cggcgacggg gatcggaggc ctcgtcttca acccggttgc caacgggctc
    10921 atcctgcact acggttggcg ggagacttat atccttcttg gcgccctcat cggcgtcatc
    10981 tgtatgccca ccgccctcct cctgatgaag cggtcgccgg aggaggaggg gtggcatccg
    11041 gatggcgcgg ggcgggcgga gggggcggcg ccggaggtgg cggccggggg ctatcggctg
    11101 ggcgagttcg ctaaaacccc ggttttctgg ctgctggccg ggatgatcct gctcgtcaac
    11161 acgctcaaca tgggcatcca gcagcacctg atcccctacc tgacggacat cggccacagc
    11221 tccacggtcg cggccaacat catggggctc tacctcggga tgacggtctt cggaaaactg
    11281 gtcctgggga gcttctgcga ccggcggggc ctgaaggcgg ggctcgcgat ctttaccgcg
    11341 atcctggtcg tcgggatcct cctgctcttc ggggcggcct cgctgccgtg ggccgtcctg
    11401 ttcggcgtgg tctatggctt tggaaacgcc atccagaccg tcctgccgcc gctgatgacg
    11461 gtggccgctg cggggctcgc ccactttgcc ctgatctacg ggattatgac catcttccag
    11521 acgctggggt cgggcatcgg catgcctctg tcgggctacc tgtacgactg gacagggggg
    11581 tatgaagcgg cgttttatgt ctatatcggc ctggccgtcc tggccgcact gctggggatg
    11641 gcggccctga agcgggcgcg cttcaaggcc gtctgaggct gccgggtccg gggggaggca
    11701 cttcggccgg aacgggacgc cccgccgggc gaacggtctt aggaaagatg gaccccgggg
    11761 attgcgcttc ccccgggtaa cgtcaagagt tttatgtcag agataagagt gagggggctc
    11821 ctgctttttc ccccgttcat tcttgacagg gtccgcgatt tttcctaagc tcccggcgct
    11881 acccggcgct gccgggtcga tgcggggagg cctctgcccc ctgtcatttt gagaagcgcg
    11941 gttatcctgt catctcgagg agcgcaagcg acgagaaatc ttcatgatgg ccggttctca
    12001 aagatttctc ccttcggtcg aaatgacata agggcaatcg aaatgacaga agggcggcgg
    12061 aaatgacaag taggccggcc gggacgatcg gaacggtcgc ggacgatgca ggcgggggag
    12121 ggagatggcg acatcccgaa aattcacggt ggccctgatc cagatggcca tgagcgccga
    12181 tcccggcgaa aacctccgtc gcgccgtgac tcaaatcgcg gctgcggcga aacggaaggc
    12241 cggagtcgtc tgccttcccg agctcttccg ctcccgctac ttctgccagc gcgaagagac
    12301 cgccgccttc gatctagcgg agacgatccc cggcccgacc accgaggccc tggccggcct
    12361 cgcccggagg caccgggtcg tcgtcatcgc cccgcttttc gaacgccggg cgccgggggt
    12421 ctatcacaac agcgtcctcg tgatcgatgc cgacggccgc ccggccggac tctaccgcaa
    12481 gatgcacatt ccggaagacc cggcctacta cgaaaaattc tacttcaccc ccggcgatct
    12541 cggctttccg gccttcgaca cgcgctgcgg gcgcatcgcg accctgatct gctgggacca
    12601 gtggtttccc gaggcggcgc gcctcgccgc gctccacggg gcggcggcgg tcttctaccc
    12661 cacggccatc gggtggcacc cccgggagaa ggcccggttc ggcgccgccc agcgcgaggc
    12721 gtggaaaacc gtccagcggg ggcacgccat cgccaacggc gtgtacgtgg ccgcggtcaa
    12781 ccgggtcggt ctcgaacggc cggaggtggg cggcgacggc atcgagttct ggggatcgtc
    12841 cttcattgcc gacccccagg ggatcgtcct cgcggaggcc tcgccggacc gggaagagat
    12901 ccttctggcc gaggtggacc tcacgcacct cgaagacgtc cggcggaact ggcccttcct
    12961 gcgcgaccgc cgcatcgatg cctacggcgg cctgatggag cgcttcctgg accatcgggt
    13021 tccggagaag aaaaaatgaa tgaggattat cgaggaggcg ggtcatgacg gccgatcgcg
    13081 cccggacccc cgccgcagag gggttccgcc tgcccgccga gtgggagccg cacgcggcga
    13141 cgtggctcgg ctggcccgcg aaccggacgg actggccggg gaagttcgcc ccgatcccct
    13201 gggtttatgg ggagatcgtg cgtaagctcg ccccgggtga gatctgccgc atcatcgcgc
    13261 agggccggat cgagcggcgg gcccggggcc tccttgcacg tgcgggcgtc gacccggccc
    13321 gcgtcgaatt cttcgatttt ccgtccgacc gcggctggac gcgcgattcc ggacccgtct
    13381 tcctcaagcg cgaggggcca cccgcggagg tcgccgtggc gggattccgg ttcaacgcct
    13441 gggcgaagta tcccgactgg gcgcgggacg ccaaaatccc cgcccgggcg gcggagcggc
    13501 tcggcctgcg cctgctgccc gcccggatcg gcgacacgga tttcgttctc gagggcggca
    13561 gcatcgacgt gaacggcgcg ggcgcgctcc tgaccacgga ggaatgcctc ctcgatcccg
    13621 cggtccaggt ccgcaacccc ggcttcgggc gggccgagat cgaacgggct ctccaggacc
    13681 acctgggcgt gacccgtatt ctctggctcg ggcggggcat cgccggggac gacacgcacg
    13741 gccacgttga cgacgtctgc cgtttcgtga acgaccgcat ggtagtgctg tgccgcgagg
    13801 cggatccgtc ggacgccaat taccgccccc tcgaagagaa ccgggagcgt ctccaggggg
    13861 tgaggctcgc cacaggcgcc tcgctcgagg tcgtttccct ccccatgccg gcgccgctct
    13921 tcctcgatgg acgacgcctg ccggcgagct acgcgaattt ctacatcgcg aacgccgcgg
    13981 tgctggtgcc gaccttcaac gatccgaacg accggatcgc cctcg
//
LOCUS       DAOOLJ010000047        13887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4790_length_13887_cov_14.859746, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000047 DAOOLJ010000000
VERSION     DAOOLJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_13887_cov_14.859746"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLW16_07770"
     CDS             <1..901
                     /locus_tag="PLW16_07770"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB70841.1"
                     /translation="NSQSTVLISGESGTGKELFANAIHYNGPRANQPLVAINCAVLSE
                     GVLESELFGHEKGAFTGAIKQKKGLFELADQGTLFLDEIGEISPATQLKLLRFLEERE
                     FQRVGGTRSIEVDVRIIAATNKNLRQNVEDGKFREDLYYRLNVISLPIPPLRERQDDI
                     PLLADYFLEKYSRLLSKNVRRLSDDARGVLFRYPWPGNVRELKNIIERIVLLSDDDVI
                     TGREIPAEIGGPSHKTELFEGGGMEEMPLDRLIKLYTEGILARHGGNRTRTAEILGIT
                     RQRLRRILKSLAPGESDPDSGSI"
     gene            1198..2007
                     /locus_tag="PLW16_07775"
     CDS             1198..2007
                     /locus_tag="PLW16_07775"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPB70842.1"
                     /translation="MVEMKDPFRVEREGHIAWLVLNRPEKRNAMGIKFYDWLGEHFAA
                     FDEDPEVRVVVIRAEGKSFTTGTDLGELGSMYNEMDAGTREKMRMTILKAQEGMSRVE
                     KCRKPVIAAVHSHCLGGGVDLLSACDIRIASADAVFSVREVKVAFIADVGTLQRLPHI
                     IGHGWFRELALTGRDFSAEEALKIGFITRICPDREALIAEARSLAEEIAACSPLAVQG
                     TKDVLLYSRDKGIYPGLEYVAQKNAAAIPSEDMIEAAMAFMEKRTPVFKGK"
     gene            2056..3618
                     /locus_tag="PLW16_07780"
     CDS             2056..3618
                     /locus_tag="PLW16_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70843.1"
                     /translation="MADHRRITEAVIEEVFVGKSCDAVVIGAGLGGMSAATFLARSGK
                     KVLLLERHHLPGGYASSFRRGRFEFDISLHVLSGVGLPEKRGRLYEYLDFLGVARKLK
                     FLPLKELYRVVDDRIDITLPPDWEGSIDRLCGFFPAEAEKIREFFALMKQLARDLSGV
                     FVAAGRKASDLTPECFPVFARYGMKTYGEILDRFFRDEDLKSAVSPFWSYLGLPPSKI
                     PFHMMASCWDALLKQHPVHIRGRNQALSNAFIETLTECGGEVRFGCGARRIVLKDGAV
                     RAVITDEDEEVATNVVVSNASIPSTLSDLVGTEQVPETYRRQVNSRQIGFSTVNIYAG
                     LDCPPEAVGATVHENFIDFGSRIEETWQTAHTLAPPRGMLFTSYTASDPEFSPPGTSV
                     IVMTAASYARPWYLVPPERYVEEKTTFAASMLDRAERYFPGLRAHLEVVEVATPLTNM
                     RYTGNPGGTIYGFDQYLSDSGLLRLQNRAPIGGLYFASAWTLPGGGYQTCMTSGFLAG
                     RMALKKLERGGS"
     gene            3615..4775
                     /locus_tag="PLW16_07785"
     CDS             3615..4775
                     /locus_tag="PLW16_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB70844.1"
                     /translation="MNIQEYLSQVDGFINVYQERQVLEQARGDLSLPRDFAARVVERL
                     HPAEIPVIVGEVREETRSAKTFRLMARDGYLPPFRAGQYIHWSVIVGGVRTGRAYSIA
                     SPPHERGFYEVTVQRQAGGFVSPFLLDEVEAGDEFSISGPAGTFYHEPLMDGDELVFL
                     AGGSGITPFRSLIRESVERGLPRRIWLLYGIRTPEDILYRRELSGIARAHDDVRVRFV
                     CSEPPPGYRGPRGLIAAAAIRRFVGPPEGKTFYVCGPEAMYRFVLPELARLSVPRRRI
                     HCEVSGPPANVAQEAGWPEAVRSDRMFQVSVAGHGTIPARAGEPLINALERSGLAVPS
                     LCRSGECGYCRMKLLSGQVFVPDRVAVRESDRWFNYIHACLTYPLSDLELRL"
     gene            5005..6534
                     /locus_tag="PLW16_07790"
     CDS             5005..6534
                     /locus_tag="PLW16_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70845.1"
                     /translation="MNIGNIPARNAIQYPDKTALIFEGERWTWRQFNSRINRLSQALM
                     GLGLAKGDKVAILTENVPAIVETNYACAKAGIVYFPVMSRLFPKDIQHLVDLSDARVL
                     IFHPDFRGVVEPMRAELPKVATYIQIGGETPPWAMAYEPFLQSGRDAAPDVEVLPDDV
                     YCFICTGGTTGVSKLGMLSHLNALAAIHTSIGAMNITPDDVGLQVLPLFHVIQNNCLH
                     PMLAAGASVVLQHRFDAVQFMKAIHEEKVSVVIVVPPFLFSWIMAVPEAIACDVSHVR
                     LFATAAATFPDDLKRDVLKYMPKAKLIYVYGLTESSGGNAVLLMHENTFRKSGAIGVV
                     NPLMDYRIVDHRGERVPVGTAGELLLKGPPVIRGYYKRDEETAATFVDGWLHTGDIVR
                     EDEEGFMYFVDRLKDMIKTGGENVFAKEVEDALLNHPKISEVAVFGLPDPQWGEKIHA
                     AVVLKPGMEATDEEILAFAKEHLAGFKRPKAIHFIAALPRNPSGKVLKHVLKQELADK
                     S"
     gene            6579..7775
                     /locus_tag="PLW16_07795"
     CDS             6579..7775
                     /locus_tag="PLW16_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70846.1"
                     /translation="MDFAFSEEELGFRREVEVFAREELPADWDDRALYWPAGYGTMSN
                     FEAEFKPFCDQFMRKMGKRGWLSIGWPVEYGGMRSMMKQAILDDVLTYYRAPSGEVST
                     IIGGHTIIGVGSDEMKRKWLPRIAAGEVRFWLGYSEPNAGSDLSSIKTSAVDRGDEFV
                     VNGQKIWSSAAHIADYAWLLAKTDPNASKHAGATLMLVDNRSPGITIRPIINICGIHS
                     FNEVFFDDVRVPKENVVGGINQGFYCVMLALQYERIFVGAGTFRRVLEELIQYVKENV
                     GERIAPCDSGGIKRKLAELATEIEVLYGYYWRTAWMMDQGLIPEIESSILKLFATELS
                     RTLASTAMDILGPYGPLERGARWAPLKGRISLGYLDAVSGPIGAGTSEVQRGIIATRG
                     LGLPRR"
     gene            7812..8957
                     /locus_tag="PLW16_07800"
     CDS             7812..8957
                     /locus_tag="PLW16_07800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70847.1"
                     /translation="MDFNLSREQRMLRDAANDFLANECPKARVREIEKSADGHDRALW
                     EKMGELGWMGLIVPEAYGGSGGGFLDLVVLLETMGYHLCPSPYFATVLLGSVPILACG
                     SEARKEALLPELAAAKRIATLALTETGGGIEAEAIRVPGRIRGSGFEIDGVKLFVSYA
                     HVADVLLCAVRTDESAVPEMGVTLFLVERDQPGISIGPMKSLGDERPCEVAFSGVRVT
                     EGEIVGGFNRGWPIVESVLGKASVALGAQMIGGAQAVVDMALQYAKERTQFGHPIGSF
                     QSIQHYFADMWADILGSRDLLYRAAWKITENLPAAGDVAMARARAGKTYRRVTTLGHQ
                     IFGAIGFTEEHDMHLYHRRSIAMDLALGGAECSYRSIANGLFDEAGA"
     gene            9069..9527
                     /locus_tag="PLW16_07805"
     CDS             9069..9527
                     /locus_tag="PLW16_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPB70848.1"
                     /translation="MEDREWFEDYTLGETLVSPGRTITETDIVLFSAFTGDWHPLHTN
                     VEYAKKTFFGERIAHGMLGLVVGSALIFRLGPHVFTPKSFIAFYGMDKIRFTGPVKIG
                     DTIRCEAVINELRERDEKTGVIRYEATIRNQRDEACLVFYPAILVGRKKA"
     gene            9553..10704
                     /locus_tag="PLW16_07810"
     CDS             9553..10704
                     /locus_tag="PLW16_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70849.1"
                     /translation="MDFQFSEDQKMFRATLKKFVDDVVIPRAPEIDQDGVFPKESFQA
                     MAELGLFGISIPPEFGGSGEGIMSCVLAMEELARGCAATANTFGAHAILCTENICRNG
                     NDEQRKKYLPDLIAGRKIGAIAITEPEAGSDALSMRTRAVRKGDRYLLNGTKMFITNG
                     PVADLVLVYAKTDPEAGAKGITAFVVERGFPGFSSGKTLKKMGVKGSPTGELIFEDCE
                     VPAENVIGAENGGVRVLMNGLDRERIVYSICPIGVAQGAFDVAFRYAQERVQFGLPIV
                     HFQMIQEMLAEMATEIHAAKLMAYWAATTADQGGRVRLEAAYAKLFCSQVGMRAVDKA
                     VQILGGYGFISEFPVERMYRDMKGIEFGAGTTQIQKLIIAQELLRKGSN"
     gene            10830..12395
                     /locus_tag="PLW16_07815"
     CDS             10830..12395
                     /locus_tag="PLW16_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70850.1"
                     /translation="MDERMAMNMMRRVAYGDCLRRATLRYPTREAVVDGGRRITFEEL
                     NRRANQFANALKGRGFQKGTKIAFISLNALEHFVAFFGTAKVGMIFVPINPLFKTDEL
                     AFALNHADCEVVIFNGLMYPGFKDAFAKAEKARLFIAFNPPEGDFVTFDAFLGEGSGE
                     EPETIIEADDDLLILYTGGTTSYPRAAVLSHLNLFVCTNGILIDIPFTHQDKLFMVMP
                     LFHVGAFIITSLVMFVGGAVHLYMLPDMKQILVNVEKEKITCTLLISPLWRQLLDHPD
                     FGKHDLSSLRLCVYFTAVMPEDLLREVMAKVCPNLCLCFGQTEMSPSTTCFKPEDQLR
                     KMKSLGNSTVNVEIAIMDDFGNLLPAGEAGEIVYRSPQVMKGYYKDEAENRRVFEHGW
                     FHSGDVGYLDEENYVYFLDRKKDMIKTGGENVASLDVEKTIYLDPRVQEVHVIGLPHE
                     RWAEAITAFVIPKPGQTIEEKEIIALCKEKMVGFKVPKRVIFLPDLPRSAVGKVLKRK
                     IRDEYVELYKGER"
     gene            12509..>13887
                     /locus_tag="PLW16_07820"
     CDS             12509..>13887
                     /locus_tag="PLW16_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB70851.1"
                     /translation="MEERPWFRHYDPGVPRRLEVPEKVCHEFLEARAAGMPEGTALTF
                     MGMPLTYREVNARSNRFAHALRAWGLKKGDRVAMVMGNSPAFVSCQFGILKAGGVVVP
                     VNPLYTGRELEHVLGDSGARLAVVLSLFVENVIAVKGGTALEAIIAVPIPGMEIPLPE
                     GTILLEAFMEGKPEHNPGLDMGSTDPALVIYTGGTTGRSKGAVLHHRSQVYCATMLST
                     LDPSMTTQQDAFLLVMPLFHIMGNAIMTFCIHDGLPIHLVPRYDPKLLLEDIHRLRPT
                     WFPGVPTIFIGVMHHPDVKDYDLTSLRYCISGAAPFPVEAMEEFEAITRCRVNECFGL
                     TEAGSAVLLNPFVNRRKFGSIGIPTPGMDARIVDLETGTRELPQGEEGELVLKGPPIM
                     MHYHNMPEETALALRDGWLHTGDIARMDADGYFWIVDRIKDMIIIGGENVYPREIDEV
                     LYEHPKVELA"
BASE COUNT         2614 a         4121 c         4658 g         2494 t
ORIGIN      
        1 caacagccag tccacggtgc tgatctccgg ggagagcggg accgggaagg agctgttcgc
       61 caacgccatc cattacaacg gcccccgggc caatcagccg ctggtggcga tcaactgcgc
      121 cgtcctgtcg gaaggggtcc tcgagagcga actgttcggc catgaaaagg gcgctttcac
      181 cggcgcgatc aagcagaaga agggcctgtt cgaattggcc gatcagggga cgctgtttct
      241 ggacgaaatc ggcgagatca gccccgccac ccagctcaag ctgctccggt ttctcgaaga
      301 gcgggagttc cagcgggtcg gggggacgcg gagcatcgag gtcgacgtcc ggatcatcgc
      361 cgcgacgaac aagaacctgc gccagaacgt ggaggacggc aagttcaggg aggacctcta
      421 ctaccggctg aacgtgatct ccctgcccat cccgcccctg cgggaacggc aggacgacat
      481 cccgcttctg gccgactact tcctggagaa gtacagccgg ctcctcagca agaacgtccg
      541 ccgtctgagc gatgacgccc ggggcgtcct cttccggtac ccctggccgg gcaatgtgcg
      601 ggagctcaag aacatcatcg agcgcatcgt gttgttgagc gacgatgacg tgatcaccgg
      661 gcgcgagatc ccggccgaga tcggcggccc gtcccacaag acggaactgt tcgaaggggg
      721 ggggatggag gaaatgcccc tggatagatt gatcaagctc tatacggaag gcatcctcgc
      781 gcgccacggc ggcaaccgaa cccggacggc cgagatcctg gggatcacgc gccagcggct
      841 gcgccggatc ctgaaatccc tggcgccggg tgaatccgat ccggactccg gttcgattta
      901 gaccatggaa tatccgcttg gacttgttgg attatttttg atgaactgac cgcggggcgt
      961 tccggcgctc gatacgcgtg gatgaagctc atccaggttt gaggtccatt gggtcaggcc
     1021 gtttcgcccg caggaggaca ttccgagtgt atgagcatgg aagaactcgt tttcagaaga
     1081 caatattaag gtgacggatc gggacggcac cgtcccggaa ggggcggcac agctcttgct
     1141 gggttggggg tgagtctcct gattgccatc ccaacgataa cgggcaggag gtgggtgatg
     1201 gtggagatga aggatccttt cagggtggaa cgggaagggc atatcgcctg gctggtgctc
     1261 aaccggcctg agaagcgcaa tgccatgggg atcaagtttt acgactggct gggggagcat
     1321 tttgccgcct ttgacgagga tcccgaggtg cgggtcgtcg tcatccgtgc ggagggaaaa
     1381 tcgtttacca ccgggacgga cctgggcgag ctggggtcca tgtacaacga gatggacgct
     1441 ggcacgcggg aaaagatgcg gatgacgatc ctgaaggccc aggagggcat gagccgggtc
     1501 gagaaatgcc gcaagccggt catcgccgcg gtgcacagcc actgtctggg gggcggggtg
     1561 gatctcctgt ccgcctgcga cattcgcatc gcgtcggccg acgccgtctt cagcgttcgg
     1621 gaggtgaagg tggccttcat cgcggatgtc ggcacgctgc agcggctgcc gcacatcatc
     1681 ggccacggat ggtttcggga actcgccctg acggggcgcg atttcagcgc cgaggaggcg
     1741 ctgaagatcg ggtttatcac ccggatctgc ccggaccggg aggccctgat cgcggaggcg
     1801 agaagcctgg ccgaggagat cgccgcgtgt tcgcccctgg ccgtccaggg gacgaaggac
     1861 gtcctcctct acagccggga caagggcatt taccccgggc tcgagtacgt cgcccagaaa
     1921 aacgccgcgg ccatcccctc ggaggatatg atcgaggccg ccatggcctt catggagaag
     1981 cggacgcccg tcttcaaggg caaataatcg ccggatccgc agggaagaac cacccgttca
     2041 cacgaagcgt ctccggtggc ggatcatcgg cgcatcaccg aagcggtcat cgaggaggtc
     2101 tttgtgggaa aaagctgcga cgccgtggtc atcggagccg gtctgggggg gatgtcggcc
     2161 gcgaccttcc tcgcccggag cggcaagaag gttctgctcc tggagcgcca tcacctcccc
     2221 ggagggtacg cgagttcctt ccggcgggga cgcttcgagt tcgacatctc gttgcacgtc
     2281 ctgtcggggg tcgggcttcc ggaaaaaaga gggcggctct acgagtacct ggatttcctg
     2341 ggggtggccc gcaaactgaa gtttctcccg ctgaaggagc tctaccgggt cgtggacgac
     2401 cggatcgaca tcaccctccc gcccgactgg gaggggtcca tcgaccggtt gtgcggcttc
     2461 tttcccgctg aggcggagaa gatccgcgaa ttctttgccc tgatgaagca gctcgcccgg
     2521 gacctgtccg gggtcttcgt cgcggccgga cgcaaggcgt ctgacctgac gccggagtgc
     2581 ttccccgtct ttgcccgcta cgggatgaag acctacggcg agatccttga ccgctttttc
     2641 cgggatgagg atctcaagag cgccgtctcg cccttctggt cgtatctggg tctgcccccg
     2701 tcgaagatcc cgttccacat gatggcgtcc tgctgggacg ccctgctcaa gcagcatccc
     2761 gtccatatcc ggggccgcaa ccaggccctg tccaacgcct tcatcgaaac cctgaccgaa
     2821 tgcggcggag aggtccgctt cggttgtggc gcccggcgga tcgtcctgaa ggacggggcg
     2881 gtccgggccg tgatcaccga cgaggacgag gaggtggcga caaacgtcgt cgtttccaac
     2941 gcctccattc cctccaccct gagcgatctg gtcgggacgg agcaggtgcc cgagacctac
     3001 cgccgccagg tgaacagccg ccagatcggc ttttcgaccg tgaacatcta cgccggcctc
     3061 gactgcccgc ccgaggcggt aggcgcgacg gtccatgaaa acttcatcga tttcggaagc
     3121 cggatcgaag agacctggca gacggcccac acccttgccc ctccccgggg gatgctgttc
     3181 acgagctaca cggcttcgga tcccgagttc tccccgccgg ggacgtcggt catcgtcatg
     3241 acggcggcca gttacgcccg gccctggtat ctcgtgccgc cggagcggta cgtcgaggag
     3301 aagaccacct tcgcggcgtc catgctcgac cgggcggagc ggtatttccc gggcctccgg
     3361 gcgcatctgg aggtggtcga ggtggcaacg cccctcacga acatgcgcta tacgggcaat
     3421 ccgggcggga cgatctacgg attcgatcag tacctttcgg actcgggcct cctgaggctt
     3481 cagaaccgcg cgcccatcgg cggcctgtac ttcgcatcgg cctggacgct gcccggcggg
     3541 gggtatcaga cctgcatgac gtccggcttc ttggcgggcc gcatggccct gaagaaactt
     3601 gaacgggggg gatcatgaat atccaggagt acctgagcca ggtcgatggc ttcatcaacg
     3661 tctatcagga gcggcaggtg ctggaacagg cccggggcga tctgtcgctt ccccgggact
     3721 tcgccgcgcg cgtggtggag cggcttcacc ccgccgagat tccggtcatc gtcggagagg
     3781 ttcgggaaga gacgcgctcg gccaagacct tccggctcat ggcccgcgac gggtatctcc
     3841 cgccgttccg ggcggggcag tacatccact ggtccgtgat tgtcggcggg gtgcggacgg
     3901 gcagggccta ttccattgcg tcgcctcccc acgagcgcgg gttctatgag gtcaccgttc
     3961 agcgacaggc cggagggttt gtctcgccgt tccttctgga cgaggtcgag gcgggcgatg
     4021 aattcagcat ttccggtccg gcggggacct tctaccatga gcccctgatg gacggggacg
     4081 aactggtctt tctggccgga ggttcgggaa tcaccccctt ccgcagtctg atccgggaga
     4141 gcgtcgagcg cggtcttccc cggcggatct ggcttcttta cggcatccgc acaccggagg
     4201 acatccttta ccgccgcgaa ctgagcggga tcgcccgcgc ccatgacgat gtccgggtcc
     4261 ggttcgtgtg ctccgaaccc ccgcccggtt atcgcgggcc gcggggcctg atcgcggcgg
     4321 cggccattcg ccgtttcgtg gggccgccgg aaggcaagac cttctatgtg tgcggtccgg
     4381 aggcgatgta ccggttcgtg ctgcccgaac tcgcccggct gtcggtgccg cgccgccgga
     4441 tccattgcga ggtgagcggc cccccggcga atgtcgccca ggaagccggc tggccggagg
     4501 cggtgcggtc cgatcggatg ttccaggtgt ccgtggccgg gcacggaacg atccccgccc
     4561 gggcaggaga gcccctgatc aacgccttgg agaggagcgg actggccgtc ccgtcgctgt
     4621 gccggagcgg cgagtgcggg tattgccgga tgaagctgtt gagcggccag gttttcgtgc
     4681 cggaccgggt ggcggtccgc gaatcggacc gctggttcaa ttacatccat gcctgtctga
     4741 cctatcccct gtcggacctg gagcttcggc tctaaactgc gatcgcacgc ccgtcgcccc
     4801 cgctgagggg gggcggacct ctcgtcggac caggattttc gtgtaacgcc ggtccggttt
     4861 ctcaatcgga acggacattc gggtcgggag accttttctc cccatcgagc ggccgaagtc
     4921 gtccctttcc ggggcggaaa tcatcggtgt cctccgggcg gcgtcctgcc gggaggacgt
     4981 catcctgatg aaggaggtgg atcgatgaac atcggcaaca ttccggcgag aaacgccatt
     5041 cagtacccgg acaagaccgc cctgatcttc gaaggggaac gctggacctg gcggcagttc
     5101 aacagccgca tcaaccggct ttcccaggcc ctgatggggc tggggctcgc gaagggcgac
     5161 aaggtggcca tcctgacgga gaacgtcccg gccatcgtcg agaccaacta tgcctgcgcc
     5221 aaggcgggga tcgtgtattt ccccgtgatg tcgaggctct ttcccaagga catccagcac
     5281 ctcgtcgatc tgtcggatgc gcgggtcctg atctttcacc ccgacttccg gggcgtcgtg
     5341 gagccgatgc gggcggagct gccgaaggtc gcgacctaca tccagatcgg gggcgagacg
     5401 ccgccctggg ccatggccta tgagccgttc cttcagtccg gccgggacgc ggccccggac
     5461 gtggaggtcc tgccggacga cgtgtactgc ttcatctgca ccggcggcac cacgggcgtt
     5521 tccaagctcg gcatgctctc gcacctgaac gccctcgcgg cgatccacac ctccatcggc
     5581 gccatgaaca tcacgccgga cgatgtcggg cttcaggtcc tgcccctgtt tcacgtcatc
     5641 caaaacaatt gtctccatcc catgctggcg gcgggggcct cggtggtgct ccagcaccgg
     5701 ttcgacgcgg tgcagttcat gaaggcgatc cacgaggaga aggtctcggt cgtcatcgtc
     5761 gtcccccctt tcctcttcag ttggatcatg gccgtgcccg aggcgatcgc ctgcgacgtc
     5821 agccatgtgc gcctcttcgc cacggccgcc gccaccttcc ccgacgatct caagcgggac
     5881 gtcctgaaat acatgccgaa ggcgaaactc atctatgtct atggtctgac ggagtccagc
     5941 ggtggcaacg cggtgctcct gatgcacgaa aacaccttcc ggaaatccgg ggcgatcggc
     6001 gtggtcaacc ccctgatgga ctaccggatc gtggaccatc ggggggaacg ggtaccggtg
     6061 gggacggcgg gggaactcct cctcaagggg ccgcccgtga tcagggggta ctacaagcgg
     6121 gacgaggaga ccgcggccac gttcgtcgac ggctggcttc atacggggga tatcgtgcgg
     6181 gaggacgagg agggattcat gtacttcgtc gaccgcctga aggacatgat caagacggga
     6241 ggagagaacg tcttcgccaa ggaggtggag gacgccctgc tcaatcatcc caagatctcc
     6301 gaggtggcgg tcttcggcct tcccgatccc cagtggggcg agaagatcca cgccgccgtc
     6361 gtcctcaagc cggggatgga ggcgacggat gaggagatcc tggccttcgc caaggagcat
     6421 ctcgccggct tcaagcgtcc gaaggcgatc cacttcattg cggcgctgcc ccgaaatccc
     6481 agcggcaagg tgctgaagca tgtcctgaag caggagctgg cggataaatc ctgagcgggt
     6541 agagaggaac ccgggacatt cgggaagggg gacggaagat ggattttgca ttttcggagg
     6601 aggaactggg cttcaggcgg gaggtggagg tctttgcccg ggaggagctt cccgcggact
     6661 gggacgaccg ggcgctgtac tggcccgccg gatacgggac catgtcgaac ttcgaggcag
     6721 agttcaagcc gttctgcgat caattcatga gaaaaatggg taagcggggg tggctcagca
     6781 tcgggtggcc ggtcgaatac ggcggcatgc gctccatgat gaagcaggcc atcctggacg
     6841 atgttctgac ctattatcgc gcgccgtccg gggaggtttc gacaatcatt ggcgggcaca
     6901 cgatcatcgg ggttggaagc gacgagatga agcggaaatg gctgccgcgg atcgccgccg
     6961 gggaggtgcg gttctggctg ggctacagcg aacccaacgc cggttcggat ctgtcctcga
     7021 tcaaaaccag cgcggtggac cggggcgatg aattcgtcgt caacgggcag aagatctgga
     7081 gcagcgccgc acacatcgcc gattacgcat ggctgctcgc gaagaccgac ccgaacgcct
     7141 cgaagcacgc gggcgcgacg ttgatgctgg tcgacaaccg aagcccggga atcacgattc
     7201 gccccatcat caacatctgc ggcatccatt cattcaacga ggtatttttc gacgacgtgc
     7261 gggtgccgaa ggaaaatgtc gtcggcggga tcaatcaggg gttctactgc gtcatgctgg
     7321 cgctccagta tgagcggatt ttcgtcggag cggggacctt ccggcgggtg ctcgaggaat
     7381 tgattcagta tgtgaaggaa aacgtcgggg aacggattgc gccttgcgac agcggaggca
     7441 tcaagcgcaa gctggccgaa ctggcgacgg aaatcgaggt gctgtacggc tattactggc
     7501 gcacggcctg gatgatggac cagggcctca tccccgagat cgaatcctcg atcctgaagt
     7561 tgtttgccac tgaactgtca cgcacgctcg cttccacggc catggacatc ctcggacctt
     7621 acggaccgct cgaacggggg gcccggtggg cgcctctcaa ggggcgaatc agcctcggct
     7681 acctggatgc cgtctccggc ccgatcggcg ccgggacgtc ggaggtacag cggggcatca
     7741 tcgccacccg gggattggga ttgccccgca gatgatcgtt tgacggacgg ctttacatgg
     7801 ggggagaggg catggacttc aatctgagca gggagcaacg gatgctccgg gatgcagcaa
     7861 acgatttttt ggcgaacgaa tgcccgaagg cgcgggtgcg cgagatcgag aagagcgcgg
     7921 acgggcacga ccgggcctta tgggagaaga tgggggaact gggctggatg ggtttgatcg
     7981 ttcccgaggc ttatggaggc agcggcggtg gctttctcga cctggtcgtc ttgctcgaga
     8041 cgatggggta tcacctctgt ccaagcccgt atttcgcgac cgttctgctg ggcagcgtac
     8101 cgattctcgc gtgcgggagc gaagcccgga aggaggcgct gttgccggag cttgccgccg
     8161 cgaagcgcat cgcgaccctg gccctgacgg agaccggcgg agggatcgag gcagaggcga
     8221 ttcgcgtccc ggggcggatc cgcgggagcg gctttgaaat cgacggggtc aagctgttcg
     8281 tttcctatgc gcacgttgcg gatgtcttgc tgtgcgcggt gcgaaccgat gagagcgccg
     8341 tgccggagat gggggtgacg cttttccttg tggagaggga tcaacccgga atctcgatcg
     8401 gcccaatgaa aagcctgggg gacgagcgcc cctgcgaagt cgccttctca ggggtgcgtg
     8461 tgacggaagg agagatcgtg ggcggattca accggggatg gccgattgtg gagtccgtgt
     8521 tgggcaaagc gtccgtcgcc ctcggcgcgc agatgatcgg gggtgcgcag gcggttgtgg
     8581 acatggcgct acaatacgcc aaggagcgga cgcaattcgg gcacccgatc gggagctttc
     8641 agtcaatcca gcattatttt gccgacatgt gggccgacat cctggggtcg agggacctgc
     8701 tttaccgggc ggcctggaag atcacggaga atctcccagc agcgggtgac gttgcgatgg
     8761 cccgcgcaag ggccggcaag acctaccggc gcgtgaccac gctgggacat cagattttcg
     8821 gcgccattgg cttcaccgaa gaacacgaca tgcatctgta ccacaggcgg tcgatcgcga
     8881 tggatcttgc cttgggaggc gccgagtgta gctatcggag catcgcgaat gggttattcg
     8941 acgaggcggg tgcttaaccg cgggaggcga acgccgtcct gcagggcaat cctggattga
     9001 gggaggatga aaccggtttt tgggaacgtg ttcccgaaag acccagatgg agaaaaggag
     9061 gaagactcat ggaggatcga gaatggtttg aggattacac gctgggagag acgctggtca
     9121 gcccggggag gacgatcacg gagacggaca tcgtgctctt ttccgcgttt accggagact
     9181 ggcaccccct ccacaccaat gtggagtacg caaaaaagac gttcttcggc gagcgcatcg
     9241 cgcatgggat gctgggcctc gtcgtcggat cggcgctgat cttccggctc gggccccacg
     9301 tcttcacgcc gaagagcttc atcgccttct acgggatgga caagatccgt ttcacaggtc
     9361 ccgtcaagat cggcgacacg atccggtgcg aggcggtgat caacgagctg cgcgaacggg
     9421 acgagaagac cggtgtcatc cggtacgagg cgacgatccg gaaccagcgc gacgaggcgt
     9481 gcctggtctt ctatccggcg atcctggtcg gccggaaaaa ggcgtaaaaa aagcgaaaga
     9541 gagggagaag tcatggattt ccagttcagc gaagatcaga agatgttccg ggccacactg
     9601 aagaagttcg tcgacgacgt cgtcatcccg agggcgccgg agatcgacca ggacggcgtc
     9661 tttcccaagg agagtttcca ggccatggcg gaactcggcc tcttcggcat ctccatcccg
     9721 ccggagttcg gcgggagcgg cgaggggatc atgtcgtgcg ttctggccat ggaggagctg
     9781 gcaaggggct gcgccgcgac ggccaacacg ttcggcgccc acgcgatcct ctgcacggag
     9841 aacatctgcc gcaacggcaa tgacgaacag cgaaaaaaat atttgcccga tctgatcgcg
     9901 ggccggaaga tcggggccat tgccatcacg gagccggagg cgggctccga cgccctgtcg
     9961 atgcggacgc gcgccgtccg gaagggggat cgctacctcc tcaacgggac caagatgttc
    10021 atcaccaacg gccccgtggc cgatttggtg ctggtctacg ccaagacgga cccggaggcg
    10081 ggcgcgaagg ggatcacggc cttcgtcgtc gagcgggggt tccccggctt ttccagcggc
    10141 aagaccctga agaagatggg ggtgaaggga tcgcccacgg gcgagctgat cttcgaggac
    10201 tgcgaggttc cggcggagaa cgtcatcggc gcggagaacg gcggggtccg ggtgctcatg
    10261 aatgggctcg accgggaacg gatcgtctat tccatctgtc cgatcggggt cgcccagggg
    10321 gccttcgacg tcgccttccg gtacgcccag gagcgcgtcc agttcggcct gcccatcgtc
    10381 cacttccaga tgatccagga gatgcttgcc gagatggcca cggagatcca cgccgcgaag
    10441 ctgatggcct actgggccgc cacgacggcc gaccaggggg ggcgcgtccg tctggaggcc
    10501 gcctacgcga agctcttctg ctcgcaggtg gggatgcggg cggtcgacaa ggccgtccag
    10561 atcctgggcg ggtacggctt catcagcgag ttccccgtgg agcggatgta ccgcgacatg
    10621 aagggcatcg agttcggcgc cgggacgacc cagatccaga aactcatcat tgcccaggaa
    10681 ctgctgcgca aggggagcaa ctgatcgtgg atccggagga gacctgcgca cgggaaaccc
    10741 aggcccgggc ggactccgga gcctcgcgtc gtaagggagt gtccaaatgc attcgtaaac
    10801 accaaagcct ataaaaggag ggaactttga tggacgagcg aatggccatg aacatgatgc
    10861 ggagggtcgc ctacggggac tgcctgagac gggcgacgtt gaggtacccg acccgggagg
    10921 ccgtcgtgga cggcggccgg cgaataacat ttgaagagct gaaccgccgg gcgaatcagt
    10981 ttgccaacgc cctcaagggg cggggtttcc agaaggggac gaagatcgcc ttcatctccc
    11041 tgaatgccct ggagcatttc gtggccttct tcggaacggc caaggtgggg atgattttcg
    11101 tgccgatcaa ccccttgttc aagacggacg aactggcctt tgccctgaat cacgcggatt
    11161 gcgaggtcgt gatcttcaac ggcctgatgt atcccggatt caaggacgcc ttcgcgaagg
    11221 cggaaaaggc caggctcttc atcgcgttca atccaccgga gggggacttc gtcacgttcg
    11281 acgccttcct cggcgagggg tcgggggaag agccggaaac cattatcgag gcggacgacg
    11341 atctcctgat cctctatacg ggcggaacga cgagctaccc ccgggcggcg gtcctgtccc
    11401 atctgaacct cttcgtctgc acgaacggca tcctcatcga catccccttc acccatcagg
    11461 acaaactctt catggtcatg ccgctgttcc acgtcggcgc cttcatcatc acgagtctcg
    11521 ttatgttcgt cggcggcgcg gtgcatctct acatgctgcc cgacatgaag cagatcctcg
    11581 ttaacgtgga aaaggagaag atcacctgca cgctgctcat ctcgcccctg tggcgccagc
    11641 tgctcgacca tccggatttc ggcaagcacg acctttcgtc gctccggctg tgcgtctatt
    11701 tcaccgccgt catgccggag gatctcctcc gggaggtgat ggccaaggtc tgccccaacc
    11761 tctgcctgtg cttcggccag acggagatgt cgccgtccac aacctgcttc aagcccgagg
    11821 accagctccg gaaaatgaag tccctgggca attcgacggt caacgtcgag atcgcgatca
    11881 tggacgattt cggcaacctg cttcccgcag gcgaggccgg ggaaatcgtc taccggagcc
    11941 cgcaggtcat gaaggggtac tacaaggacg aggcggagaa ccgccgggtt ttcgagcacg
    12001 gctggttcca cagcggcgac gtggggtatc tcgacgagga gaactacgtc tatttcctgg
    12061 atcggaaaaa ggacatgatc aagacgggcg gggagaacgt cgcctccctc gacgtggaga
    12121 agacgatcta tctcgatccc cgggtccagg aggtgcacgt aatcggcctg ccccacgagc
    12181 gctgggcgga ggccatcacg gccttcgtga tcccgaagcc cggacagacc atcgaagaga
    12241 aggagatcat cgccctgtgc aaggaaaaga tggtgggctt caaggtgccc aagcgcgtca
    12301 tcttcctccc ggacctgccc cgttccgcgg taggcaaggt cctgaagcgt aagatccggg
    12361 acgagtacgt ggagctctat aaaggcgaac ggtgatccgg cctgctccat ccgtttcccg
    12421 aacctgcggt acgccggcgt tctcccgtta tgagccggga ggacgccggg ccgagttttt
    12481 gaccgccatc ggcagaaggg agagacccat ggaagaaagg ccctggttca ggcactacga
    12541 ccccggcgtg ccccgtcgcc tggaggtccc ggaaaaggtc tgtcatgaat ttctggaggc
    12601 gcgcgctgcc ggaatgccgg agggaacggc cctgaccttt atggggatgc cgctgacgta
    12661 tcgggaggtc aacgcccgaa gcaaccggtt tgcgcacgcc ctgcgggcct ggggactgaa
    12721 aaagggggat cgggtggcca tggtgatggg caattctccg gccttcgttt cctgccagtt
    12781 cggcatcctg aaggcgggcg gcgtggtcgt gcccgtcaac cccttgtaca cgggacgcga
    12841 gttggaacac gtcctgggcg attcgggcgc ccgcctggcg gtggtcttga gtctgttcgt
    12901 cgagaatgtg atcgcggtca agggggggac ggcgctcgaa gcgatcatcg ccgtgcccat
    12961 tccgggcatg gagatccccc tgccggaagg gacgattctc ctggaggcgt tcatggaggg
    13021 aaagccggag cataaccccg gtctcgacat gggctcgacc gatccggcgc tcgtcatcta
    13081 cacgggcggc accaccggcc gttccaaggg agcggtgctc catcaccgaa gccaggttta
    13141 ctgcgccacg atgctgagca cgctggatcc gagcatgacg acgcagcagg atgcgtttct
    13201 gctcgtgatg ccgctctttc acatcatggg caacgccatc atgacgttct gcattcatga
    13261 cggcctgccc atccatctcg ttccccggta cgatcccaag ctcttgctgg aggacatcca
    13321 ccggttgcgg cccacctggt ttccgggcgt tcccacgatc ttcatcggcg tcatgcatca
    13381 cccggacgtg aaggactatg acctgacgag tctgcgctac tgcatctccg gcgccgcgcc
    13441 ctttccggtg gaggccatgg aggaattcga ggcgatcacc cggtgccggg tcaacgagtg
    13501 tttcgggctg accgaggccg gaagcgccgt cctgctgaac ccgttcgtga accggaggaa
    13561 gttcgggagc atcggcatcc cgacgccggg catggacgcc cgcatcgtcg atctggaaac
    13621 ggggacgcgt gaactgcccc agggggaaga aggggaactc gtcctgaagg ggccgccgat
    13681 catgatgcat tatcacaaca tgccggagga aacggcactg gcgttgcggg acggctggct
    13741 gcacaccggc gacatcgccc ggatggacgc ggatggatac ttctggatcg tcgaccggat
    13801 caaggacatg atcatcatcg ggggcgaaaa cgtctacccc cgggagatcg acgaggtcct
    13861 ctacgaacac ccgaaggtgg aactggc
//
LOCUS       DAOOLJ010000048        13811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4820_length_13811_cov_14.595667, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000048 DAOOLJ010000000
VERSION     DAOOLJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13811
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4820_length_13811_cov_14.595667"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..564
                     /locus_tag="PLW16_07825"
     CDS             139..564
                     /locus_tag="PLW16_07825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPB70852.1"
                     /translation="MVEASEMKSVTEIKVRGYHLDQHGHVNNIRYTEFLEEARYNLLD
                     PHEDVLSVLKTRGILLFIVNINIDFSQPAFLGDLLRIETGISKIGRKSGIIHQKVMKN
                     DLERPILSADVTFVAMNAATGKALPLEGELLAGIQKIAG"
     gene            complement(667..2472)
                     /locus_tag="PLW16_07830"
     CDS             complement(667..2472)
                     /locus_tag="PLW16_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70853.1"
                     /translation="MSIDTLPKAFLDRAQKKPQRVAMRWKNLGIWRDITWAQYLEKTK
                     WVALGLYALGVRRGDHIAIIGENKPEWLYCAFGAMCSGAIYVGIYTTSPAKECEYIVG
                     HSESVVYLCEDEEQFDKALEFRDKTPSLRKAIVWDMEGLKHFEDPMMMSFDALLELGR
                     KTDEETPGLFLDLINQTKPEDIAGIIYTSGTTGMPKGAMLSHEGYLWMGKAVCSITDI
                     NENDETISFLPLNHIFEQIFDFLVHNVIGHVVNFTENTDTVMADMIDVSPTMFHAVPR
                     IWEKYYSGIIIRIDDATWLKRNLYHLAVKIGTAYNELILQRKKPSLGLTIAYKLAYFT
                     VFWKLKKRLGFDRMRLGVSAAAPISHNILKFFQSMGIPIREGYGLTETTGGTHMSDLN
                     NFKLGYVGKALPGTEVKIAEDGEILVKHNALFKGYYKDEETTNQVLIDGWLHTGDVGE
                     VDEEGYLKITDRKKDLIITAGGKNIAPQFIENLLKFSPYINDAVIIGDRRKYVAALIV
                     IDEDNVVKYAQDHKVQYTTFASLTKTAEVIELIQREIDKVNAQVARVENIRKFRILDK
                     KLYTEDGEVTPTMKVKRKAINAQFADVIEEMYRGD"
     gene            complement(2487..3269)
                     /locus_tag="PLW16_07835"
     CDS             complement(2487..3269)
                     /locus_tag="PLW16_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70854.1"
                     /translation="MLKVRNIETWYDLIRALHGISFEVKQGDIIALLGSNGAGKTTTL
                     KTIMRLLYDLKEEQPEKGTIEFMGRRIDRKDTDQIVRMGISYVPEGREVFPELTVMEN
                     ILMGAYTRRDAKGIKEDIENIFERFPILRQRKNQEAGYMSGGEQQMLAIGRALMTRPK
                     LLMLDEPSLGLSPLLTQEIFNIIRDINQKDGVTILLVEQNVNMALKYANYAFLMENGR
                     IVRADKPEVLREDPDVKEFYLGIATETSVKGYKRYRRKVRFR"
     gene            complement(3262..4044)
                     /locus_tag="PLW16_07840"
     CDS             complement(3262..4044)
                     /locus_tag="PLW16_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB70855.1"
                     /translation="MDHFRVENLSISFGGLKAVRNVSFNVEKNSIFSIIGPNGSGKTT
                     IFNLISCIYKADQGRVILDGEDMLGHYPDWIARKGIARTFQNIELFSNATVMDNLMLG
                     RHIHMKTGIVAGAFMLSRRSRAAREEIRHREIVEGIIDFLDLQSVRDQFVANLPYGKR
                     KLVELGRALALEPKILLLDEPSAGMNTEEKEDLNIWVKDIREDYKVTILLIEHDMNMI
                     MGISDRVFAINQGEKITEGVPQEVQRHPEVIKAYLGEEGVHA"
     gene            complement(4186..5379)
                     /locus_tag="PLW16_07845"
     CDS             complement(4186..5379)
                     /locus_tag="PLW16_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB70856.1"
                     /translation="MKASRVWIFAVVAVLGLLLLAPASEAGKGFDDKEVRVGQFGPLT
                     GPAAPWGAVLRGSALRFQLVNDEGGIHGRKIKYFMRDDQYNPAQTVTVIKELVENQDI
                     FAIVGGVAGAGSIAAKDYLTKNKIIWIGPTASARELVFPQTPYLFCLTPLYEDEASIM
                     TKYLVETAKMKKIALMYQNDSYGQAGLAGMRQRLAKYKMKAVAEIPVEPTERDLASHI
                     MKIKNAGPEAVILWVAPTTAVISLKTAATIGFKPQWAGGTPLADYPMMHKISGGLWEG
                     VINTSYVEPPDSSLPVVVKYREAQKKYMPEERWGLFFMGGLLVADPLIDALKKAGKKL
                     SLEAVLKQLNSAKNYKTIGPTINWSKNQHQGVDSAMIWKCGPGGKTMVMQGWTQNELA
                     TWKKK"
     gene            complement(5526..6719)
                     /locus_tag="PLW16_07850"
     CDS             complement(5526..6719)
                     /locus_tag="PLW16_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB70857.1"
                     /translation="MRFKTTVGWMVVALAAAMIMAPAALAGKPGFDDKEIRIAAWGPQ
                     TGPAAAWGNVARGPNVMAAIVNAEGGIHGRKLKHFVRDDQYNPAQTKLVVKELVEQQG
                     IFSFVCGTSAAGGMAVKDYLKDNKVIWVGPATSVEEYVFPVNPYLFTIMPLYEDEASI
                     LTKYVVEKLKMKKIGILYQNDPYGKNGLKGVLQRLKKYRMKAVAEIPVEPTEQDLASQ
                     MQKFKSSGAEAVLMWVAPKTAVIALKTSKSMGYTPQWVSSMTLGDYELMYKISGGLWE
                     GVVTGAFTLPPETRHPLMDKYREATKKYAPEERWGVFFHAGIMFTEPLIHALKKVGKN
                     LSTEACLRELNKIRNFKGTGGKITYTPKRHQGTDSIMVMKCGPNATYIKVMDWTSNEL
                     ASWKK"
     gene            complement(6901..7317)
                     /locus_tag="PLW16_07855"
     CDS             complement(6901..7317)
                     /locus_tag="PLW16_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70858.1"
                     /translation="MGLLIAVFLLGCSDAGDRAASFFENRAVYRAVIHYLDAEVGRDH
                     KEIYESLAPSSPYCIRNTYEMYLAEAKTAPAIASYRIVRISNIRRNEDRQRYPEIDKI
                     ARVEVELVLVLDDSGQETEVNFAFPFVKERGKWYKG"
     gene            complement(7423..8295)
                     /locus_tag="PLW16_07860"
     CDS             complement(7423..8295)
                     /locus_tag="PLW16_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB70859.1"
                     /translation="MIGQLIIEGLAIGACYGLFAIAVVIIYKTSEVVNFAQGEMAMLS
                     TFIAFHILNYYHYPFWLAFILTMAFAILLGVLIEFLFLRPAKHPSLLGLIVITLGAEM
                     LLMGLAGWKWGAEQKEFVVPMSYQVTHEPFQGMIINDWAIAVFVTSALVMVLLFLFFK
                     YTKLGIAMRATQQNKHAAKIMGIRVERVFSIAWGFSSLIGTIAAMFFASRSTLDPGFM
                     LEPFLRAFAAAVLGGLMSIPGVFIGGELLGLIENFFGYVWPEWKPIVAFAVIVLVLCF
                     RPSGLFAKHYVKKV"
     gene            complement(8392..9462)
                     /locus_tag="PLW16_07865"
     CDS             complement(8392..9462)
                     /locus_tag="PLW16_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB70860.1"
                     /translation="MDMKRDYFEDVQLFESNVVLFWSVALMLLLFAIPFFAPKYYIFL
                     LNIVMVHVILAVGLNVLVGYTGQISLGHAGFFAIGAYGTALLMLKLQIPFVVAILLAG
                     FIAAFFGFLLGLPALRLEGPYLAVASLGFGLAIMHVIGHWQFFGGRTGIQAPPLDIGI
                     PQLNASFILQTDAQKYYLILVIAILMIIAARNIMKTRVGRSFVAIRDSDIAAEVIGIN
                     LTIYKTLAFAISAFYAGIAGGLFGFVLGFFDPFTFNMILSIIFLVMVVVGGLGSIAGP
                     IMGAVLITYLQYSLLKNVAEVPVVGDFLVTVSSRWFSVMGLENINSIVLGLIMIGIVI
                     FEPLGMYGIWIRIKKYWMTWPF"
     gene            9850..10035
                     /locus_tag="PLW16_07870"
     CDS             9850..10035
                     /locus_tag="PLW16_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HPB70861.1"
                     /translation="MKYFLCVLGMVLIIEGLPYFAFPDKIKQYLIRIHEVPDQTLRIL
                     GLVATVAGLLFVYFGRQ"
     gene            10150..11196
                     /gene="queA"
                     /locus_tag="PLW16_07875"
     CDS             10150..11196
                     /gene="queA"
                     /locus_tag="PLW16_07875"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPB70862.1"
                     /translation="MSMDLTDFTYELPNTLIAQRPCPVRDASRMMVLHRQEQRIEHRQ
                     FFELPDYLEKGDVLIVNDSRVIPARLYALKETGGTVEVLLLKKLQEAGPDTQTWEALL
                     KPARRAAAGQVLSLPEGGRAVIRERVSDKKWAVEFSTGTDFAVYLRRNGEAPLPPYIK
                     RRPGDASHFPDRDRYQTVYARADGSVAAPTAGLHFTPRTFDALRSRGVSVAAVTLHVG
                     YGTFLPVTASRIEEHVMEEEFFEIGAEAAEAINAARRVVAVGTTATRVLEAAADERGR
                     IHPTAGTTRLFIYPGYRFKRVNRLLTNFHLPASSLLLLVSAFSGRECILDAYRQAVEA
                     SYRFYSYGDCMLIL"
     gene            11297..12454
                     /gene="tgt"
                     /locus_tag="PLW16_07880"
     CDS             11297..12454
                     /gene="tgt"
                     /locus_tag="PLW16_07880"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB70863.1"
                     /translation="MAAFHFQILKKDTRSAARRGVMVTPHGTVRTPAFMPVGTQGTVK
                     GLTPERLRELGVEMILGNTYHLTLRPGHELIRHLGGLHRFMNWPGPILTDSGGFQIYS
                     LGALRKITADGALFQSHIDGSRHMLTPERAVEIQEALGADVMMCLDECTPYPATWEQA
                     EASLNLTLVWAARSQAARTREDQALFGIVQGGMHLPLRRRSVEALADIGFDGYALGGL
                     SVGEAKDQMHGVVREIAPLLPEGRPRYLMGVGTPEDIVNCVAYGIDMFDCVMPTRCAR
                     NGLLFTNGEKVVIKHARHREDPSPLDCLCDCYTCRHYSRAYLRHLYMAGEILAMVLNT
                     IHNVRHYQRLMERIRQAIEEERYDTFRAQFMDEQKRGGGPTPRNNHSQSGP"
     gene            12469..12789
                     /gene="yajC"
                     /locus_tag="PLW16_07885"
     CDS             12469..12789
                     /gene="yajC"
                     /locus_tag="PLW16_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPB70864.1"
                     /translation="MTDIAYAMGSAGGGAAGGGDLSFIVMMAILFAIFYFLIIRPQQK
                     KQKELKEMLANLKHGDVVVTTGGIHGKITALTETIVTLEIADKVKIKVSRGFISAVLP
                     KGDQ"
     gene            12811..>13811
                     /locus_tag="PLW16_07890"
     CDS             12811..>13811
                     /locus_tag="PLW16_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPB70865.1"
                     /translation="MSGIKWRAALAVAICLGALFFLTPTLVADLPTFWNDHLPTDKIH
                     LGLDLQGGMHLVLEVDTDKAVDGTLLRTAGDLKETLMSKRVRFRQTQAAKTGLITYEL
                     PDGASRTAFEKVLKDNYPDIEVAVSEAREGREVVSLKINDKRSGEIRKLAVEQSLETI
                     RNRVDQFGISEPEIIPQGDDRIIIQLPGIKDTQRAKNLIGRTALLEFKLVNEEYSVDE
                     ALRGNVPEGSMILHESRLDRESGRRIDTPILLKSATLMTGESLESAKVQISDRFGEPH
                     VAIKFNAQGAKEFERITGANVKRRLAIVLDGVVHSAPVIQERIGGGNAQITGAFTMD"
BASE COUNT         2954 a         4111 c         3796 g         2950 t
ORIGIN      
        1 taattagtat taaatttggc gcggaaatgc tttcctcagg gctttaagct cctaatgctt
       61 cgtcgcggag gagatgacgc aactacccaa ctgatgcctg tctgccgttg ttcataaatt
      121 tgagtcaaga atattcatct ggtcgaggct tcagaaatga aaagtgttac cgaaatcaag
      181 gttcgcggct atcacctgga tcagcacggg cacgtcaata acatacgcta tacggaattc
      241 ttggaggaag cccgttacaa ccttctcgac ccacacgaag acgtgctctc cgtattgaaa
      301 acgagaggga tcctgctctt tattgtcaac atcaacatcg atttttcaca accggccttt
      361 ctgggagatc tgttgagaat cgagaccggg atttctaaaa tcggtcgtaa aagcggaatc
      421 atccatcaaa aggtcatgaa gaacgacctc gaaagaccga tcttgtcagc cgacgttacc
      481 tttgtggcga tgaacgccgc cacgggcaag gcattgccgc tggaaggcga actgttggcg
      541 ggtattcaga agatcgcagg ctaacagagg gattgccccg caacagggaa ggtgctcaag
      601 gataacaccc gtctgtcttg acgttgcgcg accccctcgt ggcggcggcg cgacattcaa
      661 cctctttcaa tcacctcgat acatttcttc tatgacatcg gcgaactggg cattgatcgc
      721 cttgcgcttt accttcatcg tcggggtcac ctcgccgtcc tcggtgtaga gcttcttatc
      781 cagaatcctg aacttccgga tgttctcgac ccgggctacc tgtgcgttca ccttgtcgat
      841 ctctcgctgg atcagttcga tcacttcagc ggttttcgtg agtgacgcaa aggttgtgta
      901 ctggaccttg tgatcctggg cgtatttgac tacgttgtct tcatcgatga cgatcagggc
      961 cgcaacgtat tttcgccggt cgccaatgat cacggcatcg ttgatgtaag gcgagaactt
     1021 cagcagattc tcgatgaatt ggggcgcgat gttcttcccg ccggccgtga tgatcaggtc
     1081 cttcttgcgg tcggtaatct tgaggtaccc ctcttcatcc acctcgccga catcacccgt
     1141 gtgcaaccag ccgtcgatca agacctggtt tgtggtttcc tcgtccttgt agtacccctt
     1201 gaatagggcg ttgtgtttga ccaagatctc gccgtcctcg gcaatcttca cttccgttcc
     1261 gggcaacgcc ttgccgacgt agccgagctt gaaattgttg aggtcggaca tatgggtacc
     1321 cccggttgtc tccgtcaatc catacccctc acggatcggg atccccatgc tctggaaaaa
     1381 cttcagaata ttatgggaga tcggcgccgc agctgaaacc cccaagcgca tgcggtcgaa
     1441 tccgaggcgc ttcttcaatt tccagaaaac ggtgaaatag gcgagtttgt aggcaatcgt
     1501 cagccccaat gatggtttct tcctctggag aatcagctca ttataggcgg tgccgatctt
     1561 gaccgccaga tgatagaggt ttcgcttcag ccaggtagcg tcgtcgatgc ggatgatgat
     1621 gccggagtaa tacttttccc agatgcgcgg cacggcgtgg aacatcgtgg gagacacgtc
     1681 gatcatgtcc gccatgacgg tatcggtgtt ttccgtaaag ttgacgacgt gtccaatcac
     1741 gttatgcacg agaaaatcga agatctgttc gaagatgtgg ttcagcggca gaaaggagat
     1801 ggtttcgtcg ttctcattga tgtcggtaat gctgcagacc gctttcccca tccacagata
     1861 gccttcatga ctcaacatgg cgcctttcgg catccccgtc gttcccgacg tatagatgat
     1921 gcccgcaatg tcttccggct tggtctgatt gatcagatca aggaagagcc ccggggtctc
     1981 ttcatccgtt ttccggccga gctccaacaa cgcatcgaag ctcatcatca tgggatcctc
     2041 gaaatgcttc agcccctcca tgtcccagac gatcgccttt ctcaaactcg gcgtcttgtc
     2101 cctaaattcg agcgccttat cgaactgttc ttcgtcctcg cagagataaa cgaccgactc
     2161 cgaatgcccg acgatgtatt cgcactcctt ggcggggctg gtggtataga tgccgacata
     2221 gatcgctccc gagcacatgg ccccgaaggc gcaatagagc cattccggtt tgttctcgcc
     2281 gatgatggcg atatggtccc ccctccgcac accaagggca tacaggccaa gggcgaccca
     2341 tttcgttttc tccaggtact gggcccaggt gatatctctc cagatcccca ggtttttcca
     2401 gcgcatggcc acgcgctggg gtttcttttg ggcccgatcc aaaaatgcct ttggcaacgt
     2461 atcgattgac atcatcctct cccctattaa cggaaccgca cctttctccg ataccgcttg
     2521 tagcccttga ccgatgtttc cgtcgcgatg ccgagataga attccttcac gtccggatcc
     2581 tcccggagga cttcaggctt gtccgcccgg acaatacggc cattttccat caggaaggcg
     2641 taattcgcgt acttcaaggc catattgacg ttctgctcca cgaggagaat cgtcaccccg
     2701 tccttttgat tgatatcccg gatgatattg aagatctctt gcgtcaggag cggggaaagg
     2761 cccaaggacg gctcgtcgag catcaggagc ttgggccggg tcatcagcgc ccgaccgatg
     2821 gccagcatct gctgctcgcc gccgctcata tagccggctt cctgattttt tctttgtctt
     2881 aaaatgggga aacgttcaaa gatgttctcg atatcctcct tgatgccctt cgcatcgcgc
     2941 cgcgtatagg cgcccatgag gatgttctcc atcacggtca gttccggaaa cacctcccgg
     3001 ccttcgggga cgtaactgat acccatgcga acgatctgat ccgtatcctt gcggtcgatg
     3061 cgccggccca tgaattcgat cgttcctttt tcgggctgct cttccttgag gtcataaagc
     3121 aggcgcatga tcgtcttcaa ggtcgttgtc ttcccggcgc cgttggaacc gaggagggcg
     3181 atgatgtctc cctgcttgac ctcgaaggaa atgccgtgca gggcccggat gagatcgtac
     3241 caggtttcga tgttcctgac tttaagcatg gaccccctcc tccccaagat aggccttgat
     3301 cacttcggga tgacgctgaa cctcctgcgg gacaccctcc gtaattttct ccccctggtt
     3361 gatggcaaag acccggtcgg agatccccat gatcatgttc atgtcgtgct cgatgagcag
     3421 gatcgtgacc ttgtagtctt cccggatgtc cttgacccag atgttgaggt cctctttctc
     3481 ctccgtgttc atcccggcgg agggttcgtc cagcaggaga atcttgggtt cgagggccag
     3541 tgcccgaccg agctcgacca gtttccgttt cccgtagggc agattggcga cgaactgatc
     3601 ccggacggac tgaagatcga gaaaatcgat gatcccctcc acgatctccc ggtgccggat
     3661 ctcctcgcgg gcggcgcggg atctcctgga taacatgaag gcgccggcaa cgatgcccgt
     3721 cttcatgtgg atatggcgcc ccagcattaa attgtccatg acggtcgcat tggaaaagag
     3781 ctcgatgttc tgaaaggtac gggcgatgcc cttccgggcg atccagtccg gataatgacc
     3841 cagcatgtct tccccgtcca ggatcacccg tccctgatcc gccttgtaga tgcagctgat
     3901 caggttgaag atcgtcgtct tgcccgaacc gttcggaccg atgatcgaga agatcgagtt
     3961 cttttcgaca ttgaaactga catttctgac cgccttgagg ccgccgaagc tgatgctcag
     4021 attttcaacc ctgaaatggt ccatcgtaat gcatatccct tcgttgatag aggttgaacg
     4081 ccgcgaaagg tcaagcgttg agggggccgt ccatcaggcc ggcgcccctc ggtgcgctca
     4141 aggacaaacc gccctgcctg taaaaagcgc gggcgtcgac atcgactact tcttcttcca
     4201 cgttgccagt tcattctgcg tccatccctg cataaccatc gtcttgccgc cgggaccgca
     4261 cttccagatc atggccgaat ccaccccctg gtgctgattt ttgctccagt tgatcgtcgg
     4321 accgatggtc ttgtaatttt tcgccgaatt gagctgcttg agaaccgctt ccaggctcag
     4381 tttcttcccc gccttcttaa gggcgtcgat cagcggatca gccacgagca gcccacccat
     4441 gaagaaaagc ccccagcgtt cttccggcat atatttcttc tgcgcctccc ggtatttgac
     4501 gacaacgggc aatgacgaat ccggcggctc gacatacgag gtgttgatga cgccttccca
     4561 gagcccgccg gagatcttgt gcatcatggg atagtcggcc aggggcgttc ctcccgccca
     4621 ctggggcttg aatccgatcg tggccgcggt cttcaggctg atgacggcgg tcgtcggcgc
     4681 cacccagagg atgacggcct ccggaccggc attcttgatc ttcatgatgt gggacgcaag
     4741 atccctctcc gtcggctcca cgggaatttc cgcaacggcc ttcatcttgt atttggcgag
     4801 gcgctgacgc atcccggcca gacccgcctg gccgtaggaa tcgttctggt acatcagggc
     4861 gatcttcttc atcttcgcgg tttcgaccag atatttggtc ataatgctcg cctcgtcctc
     4921 gtagagcggc gtcagacaaa acaggtaggg cgtctgggga aagacgagtt cgcgggccga
     4981 ggccgtcggt ccgatccaga tgatcttgtt cttggtgagg taatcctttg cggcgatgct
     5041 cccggccccg gccacaccgc caacgatggc gaagatgtcc tgattctcga ccagttcctt
     5101 gatcacggtc acggtctgcg ccgggttgta ctgatcgtcc cgcatgaagt acttgatctt
     5161 tcgaccatgg atgccgcctt cgtcattgac gagttgaaaa cggagcgcgc tgccgcgcag
     5221 gaccgccccc cagggcgcgg cgggtcccgt cagggggccg aactgaccga cccggacctc
     5281 cttgtcgtca aaccccttcc ccgcctcgga agctggcgcc aggagcaaca gtcccagcac
     5341 cgctacgacc gcaaaaatcc acacacgact tgctttcata acccctccct tattttatct
     5401 cgggcaattc cggcccggtt ccctcccctt cagccatcct tcccggaggc ggtcacccgc
     5461 gcttccgctg gtccatcatc gaaccagggg aatcggcgat tcacgcatcc cgaattgaca
     5521 gcctgttatt tcttccagct ggcgagttcg ttggatgtcc agtccatcac cttgatatag
     5581 gtcgcattgg gaccgcattt catgaccatg atggagtccg tcccctgatg ccgcttcggc
     5641 gtgtaggtaa tcttcccgcc ggtacccttg aaattcctga tcttgttgag ttccctgagg
     5701 caggcctccg tgctcaggtt cttgccgacc ttcttcaggg catggatcaa cggctcggtg
     5761 aacatgatcc ccgcatggaa gaaaacgccc caacgttcct ccggcgcgta tttcttcgtc
     5821 gcctcgcggt acttatccat caggggatgc ctggtttcag ggggcagggt gaaggcgccc
     5881 gtaaccacgc cctcccagag accgccggag atcttgtaca tcaattcgta atcgccgagc
     5941 gtcatcgacg agacccactg gggggtgtag cccatggact tgctcgtctt gagcgcgatg
     6001 acggccgtct tgggcgccac ccacatcaaa accgcttccg caccggaact cttgaatttc
     6061 tgcatctggg aagccagatc ctgctccgtg ggctcgacgg ggatctcggc cacggccttc
     6121 atcctgtatt tcttcaggcg ctgcaggacg cccttgaggc cgttctttcc gtaaggatcg
     6181 ttctgataga ggatgccgat cttcttcatt ttcagttttt cgacgacata tttcgtcaaa
     6241 atactggctt catcctcata cagaggcatg atcgtgaaca ggtagggatt gacggggaac
     6301 acgtactcct cgacggacgt ggccggaccg acccagataa ccttgttatc cttcagataa
     6361 tccttgacgg ccatgccgcc cgcggcggac gtcccacaga cgaacgagaa tattccctgc
     6421 tgttcgacga gttccttcac gacgagtttc gtctgcgcgg ggttgtactg atcgtcacgc
     6481 acgaagtgtt tcagcttccg gccgtgaatc cccccttcgg cgttcacgat ggcggccatg
     6541 acattggggc cgcgcgccac gtttccccag gcggccgccg gtcccgtctg cggaccccag
     6601 gcggcaatcc gaatttcctt gtcgtcgaat cccggttttc cggcgagcgc ggctggcgcc
     6661 atgatcatcg cggcggccag cgccacaacc atccatccaa cagtcgtctt gaacctcatg
     6721 tacgtctctc ctttccggta tcggatacat ttcccccaac agtaatgcgg accgttcaaa
     6781 ggttccgcat cactgttcct cgcgcacgtc ttcccggacg tcgcccagat ccaaatctct
     6841 tcattcaccc ccttccctct ttattatctt gattcaatgc agtattcgtc tatgaggcct
     6901 tcatcctttg taccatttcc ccctctcctt gacgaaaggg aaggcaaaat tgacctccgt
     6961 ttcctgcccg ctgtcatcca ggacaagaac cagttccacc tcgacccggg caatcttgtc
     7021 gatctccggg tagcgctggc gatcttcatt tcggcggata ttggatattc tgacgatccg
     7081 gtaggaggcg atcgccggag cggtctttgc ctccgcgagg tacatctcat aggtattgcg
     7141 aatgcaatag ggcgaggacg gcgccaggct ctcgtatatc tccttgtggt cccgtccgac
     7201 ctccgcgtcc agataatgaa tcacggcccg gtagacggcc cggttttcga aaaaagaagc
     7261 ggcccgatcg ccggcatccg agcagccgag gaggaagact gcgatcagga gacccaccgc
     7321 aacgcgtccc gaaaagcgcc ctagccgcga cgcgggacgt acctcaccga aatcggaatc
     7381 ttgataaaga atgctcacgg atacgctcct aaagcagggg gtctacacct tctttacata
     7441 gtgtttggca aaaagcccgc tcgggcggaa acacaggacg agaacaatca cggcaaacgc
     7501 gacgatgggc ttccattcgg gccagacata accaaaaaag ttctcgatca gccccaggag
     7561 ctccccgccg atgaataccc cagggatgct catcaacccg cccagcaccg ccgcggcgaa
     7621 cgcccgcaga aacggctcca gcataaaccc cggatcgagc gttgagcggg atgcgaaaaa
     7681 catggccgcg atagtgccga tcagcgagct gaacccccag gcgatggaaa atacgcgctc
     7741 gacccggatg cccatgatct tggccgcatg cttattctgt tgcgtggcgc gcatggcgat
     7801 acccagcttg gtgtacttga agaacaggaa gagcagaacc atgaccagtg cggaggtgac
     7861 gaagacggcg atggcccagt cgttgatgat catgccctga aagggctcgt gggtgacctg
     7921 gtaggacatg ggaacgacga attccttctg ttcggcgccc catttccacc cggccagccc
     7981 catcagcagc atttcggcgc cgagggtgat aacgatcagt cccagcaggc tgggatgctt
     8041 ggccggcctc agaaacagga attcaatcaa aacgcccagc aggatcgcaa acgccatggt
     8101 caggataaac gcgagccaga aggggtagtg ataatagttg aggatatgga aggcaatgaa
     8161 ggtcgacagc atggccattt cgccctgcgc gaaattgacg acttccgagg ttttatagat
     8221 gatgaccaca gcaatggcga agagtccgta acacgccccg atggccaagc cttcaatgat
     8281 gagttgtccg atcatctgtt ccccctgttg tcatgaacac cgtatttcaa cgtttggaac
     8341 gtcctgattt catgaggtcg cggggttctc tcccgcatcc cccgttcagc atcagaacgg
     8401 ccaagtcatc cagtacttct tgatccggat ccagatcccg tacatgccca agggctcgaa
     8461 gatcacgatc ccgatcatga tgagccccag gacgatgctg ttgatgttct caagtcccat
     8521 gacggagaac caccggctgg acacggtcac caggaaatcc ccgacgacgg gcacttcggc
     8581 cacgtttttg agcaggctgt actggagata agtgatcagc accgcgccca tgatcggccc
     8641 ggcgatcgaa ccgagccccc cgacaacaac catcaccagg aaaataattg ataatatcat
     8701 gttgaaggtg aagggatcga aaaacccgag aacgaaaccg aaaagtcctc ccgcaatccc
     8761 ggcgtagaag gcactgatgg caaacgccag ggttttgtag atcgtcagat tgattccgat
     8821 cacttccgcc gcgatgtcgc tatcacggat ggcgacaaaa gagcggccca cccgcgtctt
     8881 catgatgttg cgggcggcaa tgatcatcag aatcgcgatg acaaggatca ggtagtattt
     8941 ctgggcatcc gtctgcagga tgaaactggc gttcaactgc gggatgccga tgtcgagagg
     9001 aggcgcctgg atgccggttc gccccccgaa gaactgccag tgtccaatga catgcatgat
     9061 ggccaaaccg aatcccaggg aggccactgc cagataaggc ccctccaggc gcagagcggg
     9121 cagacccagc aggaatccga aaaaggcggc gataaatccg gcgagcagaa tggcgaccac
     9181 gaaggggatc tggagcttga gcatcagcag ggccgtgccg taagcaccga tggcaaagaa
     9241 tccggcatgt cccagagaga tctgccccgt ataaccgacc aggacattca gtccaaccgc
     9301 cagaatgacg tgaaccatga cgatattcaa cagaaaaatg taatacttgg gcgcgaagaa
     9361 cggaatggca aagagcagca acatgagcgc caccgaccag aagagcacca cattactttc
     9421 aaacagttga acatcctcaa aataatcacg cttcatatcc atagcaatac ctcatcaacc
     9481 ggaaatggtt tttcatcccg tttagcaaaa cggttctttt cgggcagcga ccggccagct
     9541 gcatgaaaac gcgccggcat ggatcagaaa gactgaccag tcatcgctgc cttcgaacac
     9601 ggtaagtttc ttctgccaaa gaatcaaacc aacacgtttt gaaaggtcgg ccggaaggtc
     9661 gctctcagga tgtatggaag ataaaaagaa ttaaaaaagc ggaaagccca ggcccccccg
     9721 aagcctgagc gttgatagca gaggcctacc tgccctgtca agcggaaaga gtccctcaag
     9781 gaatgggttg tcatgcgacg caggggtgtg ctataggtgc gccaacaaag caggggattc
     9841 cttagatcca tgaaatattt cctttgtgtc ctggggatgg tcttgatcat cgaagggctg
     9901 ccctatttcg ccttcccgga taaaatcaag caatatctca tccgaatcca tgaagtcccg
     9961 gatcaaacgc tccggatcct gggtttggtc gcaacggtcg ccggactgct cttcgtgtat
    10021 ttcggacgtc aatagcggga tgtcccgcat catggagcgt cgcccggcag gcggcatgaa
    10081 agggggagaa gccttcaggg catcctgtca aattccggta ccgaagcagg cttggcagga
    10141 tgagagggca tgtccatgga tttgaccgat tttacctatg agttgccgaa cacactgatc
    10201 gcgcagcgtc cctgtccggt tcgcgacgca tcccgaatga tggtccttca ccggcaggag
    10261 caacggatcg agcatcgaca gtttttcgaa ttgccggatt atctggaaaa gggcgacgtg
    10321 ctgatcgtga acgattcgcg cgtcatcccc gcccgcctat acgcactcaa ggagacgggc
    10381 ggcacggttg aggtgctgct cctgaagaag ctgcaggaag cgggcccgga cacgcaaacc
    10441 tgggaggccc tgctgaaacc cgcccgtcgc gccgccgccg gtcaggtgct gtctctgccg
    10501 gaggggggcc gggccgtcat ccgggaacgc gtatcggata aaaaatgggc cgttgaattc
    10561 tcaaccggca cggacttcgc cgtttacctg cgccggaacg gagaggcccc gcttccccct
    10621 tacatcaagc gacgtcccgg agacgcctcc cattttcccg accgcgaccg ctatcagacc
    10681 gtctatgccc gcgccgacgg ctccgtcgcc gcgccgacgg ccggactgca cttcacgccc
    10741 cgaaccttcg atgccctgcg ctcacgcggc gtttcagtcg ccgcggttac cctgcatgtc
    10801 ggttacggca cctttctccc cgtgacggcc tcccggatcg aggaacatgt catggaggag
    10861 gagtttttcg agatcggcgc cgaggcggcg gaagcgatca atgcagccag gcgggtggtc
    10921 gccgtgggga cgaccgcgac acgggtactc gaagctgcgg ccgacgaacg cgggagaatt
    10981 cacccgaccg cgggcaccac gcgcctgttc atttatcccg gctaccgctt caagcgcgtg
    11041 aaccggctgc tcacgaattt tcacctgccg gcctcctcgc ttctgctgct cgtatccgct
    11101 ttctcgggcc gggaatgcat tcttgacgcc taccgtcagg ccgtcgaggc ctcctaccgg
    11161 ttttacagct atggggactg tatgctgatt ttatgagccc gggcaacggg cgtgaagccc
    11221 ggagcgacgt gcaaaacaaa aggcggcgct cgggcggctt gcgttagaac aaaagacagg
    11281 agccaggaat cggatcatgg cggcgtttca tttccaaatc ctcaaaaagg acacccgttc
    11341 ggcggcacgg cgcggagtga tggtcacccc ccacggcacg gtgcggacgc cagcgttcat
    11401 gccggtcggc acacagggaa ccgtcaaggg tctcaccccg gagcgcctcc gcgagctcgg
    11461 cgtcgagatg atcctcggca acacctatca cctcaccctg cggccgggac acgagctcat
    11521 ccggcatctg ggcggactgc accggttcat gaactggccg ggtcccatcc tcaccgacag
    11581 cggcggcttc caaatttaca gcttgggcgc cctgcgcaag atcaccgccg acggggcgct
    11641 tttccagtcc cacatcgacg gttcccgtca catgctcacg ccggagcggg ccgtcgaaat
    11701 ccaggaagcc ctgggcgcgg atgttatgat gtgcctcgac gaatgcacgc cctacccggc
    11761 cacgtgggag caggcggaag cctccctgaa tctcacgctg gtctgggcgg cccgcagtca
    11821 ggcggctcgg acacgggagg atcaggccct tttcggcatt gtccagggcg gcatgcacct
    11881 gcccctgcgc cgccggtcgg tcgaggcgct cgcggacatc ggtttcgacg gctacgcctt
    11941 gggcgggttg agcgtcggcg aagcgaagga ccagatgcac ggcgtcgtcc gcgagatcgc
    12001 ccccttgctg cccgaggggc ggccccgcta tctcatgggc gtcggcacac cggaagacat
    12061 cgtgaactgc gtcgcgtacg gaatcgacat gttcgactgc gtgatgccga cccgatgcgc
    12121 ccgaaatgga ttgctattta caaatggcga aaaggttgtt ataaagcacg cccgccacag
    12181 ggaggatccc tctcccttgg actgtttatg tgattgttac acttgcagac attattctcg
    12241 cgcctatctt cggcacctgt acatggccgg cgaaatcctg gccatggtgc tgaacacgat
    12301 ccacaacgtc cgtcactacc agcgattgat ggagcggatc cggcaggcca tcgaggaaga
    12361 gcgctacgat acgttcagag cgcaatttat ggacgaacag aaacggggag gggggccgac
    12421 ccccaggaat aatcattccc aatcaggccc gtgaaggagg atttgacgtt gacagacatc
    12481 gcctatgcca tggggagcgc cggcggcggg gcggccggcg gcggagatct cagctttatc
    12541 gtcatgatgg ccatcctgtt tgccatcttt tactttctca tcattcgccc gcagcagaaa
    12601 aaacagaagg aactcaagga gatgctggca aatctcaagc atggtgacgt ggtcgtgacg
    12661 acgggcggga tccacggaaa aatcaccgcc ctgacggaga caatcgtgac gctcgaaatt
    12721 gccgacaagg tcaagatcaa ggtctcccgg ggattcatca gcgccgtcct gcccaagggc
    12781 gatcagtgag gaaaggggta acggattatg atgagcggca tcaaatggcg cgccgccctt
    12841 gcggttgcga tctgcctggg ggcattgttc tttctgacac cgacgctggt cgccgatctt
    12901 ccgacgttct ggaacgatca ccttcccacg gacaagatcc atctgggcct cgatcttcag
    12961 ggcgggatgc acctcgttct tgaggtggat accgacaagg cggtggacgg cacgctcttg
    13021 cggacggccg gcgatcttaa ggagaccctg atgtcgaaac gggtgcgctt ccgccagacg
    13081 caagccgcca agaccggcct catcacctac gaactgcctg acggcgcctc ccgcaccgcg
    13141 tttgaaaagg tcctcaagga caactatccc gatattgaag tggccgtctc cgaagcccgc
    13201 gagggacgtg aagtggtctc cctcaagatc aacgacaagc ggtccggcga gatccggaaa
    13261 ttggcggtcg aacagagcct cgaaacgatc cgcaaccgcg tcgatcagtt cggcatctcg
    13321 gaaccggaaa tcatccccca gggtgacgac cgcatcatca tccagttgcc cggcatcaag
    13381 gacacccaac gggccaaaaa tctgatcggc cgtacggccc ttctggaatt caagctggtg
    13441 aacgaggagt attccgtcga cgaggccctg cggggcaacg taccggaggg cagcatgatc
    13501 ctccatgaat cccgcctcga tcgcgagtcc ggacggcgaa tcgacacccc gattttgttg
    13561 aaaagcgcca cccttatgac gggggagtcc cttgaaagcg ccaaggtcca gatatcggac
    13621 cgtttcggcg aaccccacgt cgcgatcaaa ttcaacgccc agggggccaa ggagttcgag
    13681 cgcattacgg gggcaaacgt caagcggcgc ctcgcgatcg ttctggacgg cgtcgttcac
    13741 tcggcacccg taatccagga gcggatcggc ggcggtaacg cccagattac gggggccttc
    13801 accatggatg a
//
LOCUS       DAOOLJ010000049        13644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_4927_length_13644_cov_19.404298, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000049 DAOOLJ010000000
VERSION     DAOOLJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4927_length_13644_cov_19.404298"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLW16_07895"
     CDS             complement(<1..323)
                     /locus_tag="PLW16_07895"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPB70866.1"
                     /translation="MEGLYLVTDRDLCGRRSLTDVVLASVRGGARCVQLREKTLSTRQ
                     FVEEALAVRDLLTPFNVPLIINDRLDVALAVGADGVHIGQNDMPYTLARRLLGPRAII
                     GVSVET"
     gene            complement(346..1158)
                     /gene="thiM"
                     /locus_tag="PLW16_07900"
     CDS             complement(346..1158)
                     /gene="thiM"
                     /locus_tag="PLW16_07900"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPB70867.1"
                     /translation="MEPTATSIWEAVTAIRRQAPLVHNITNYVVMNNTANALLALGAS
                     PVMAHAENEASEMAVLAGALVVNIGTLSDPWIRAMRLALPAARARHIPVVLDPVGAGA
                     TAYRTQTVRELISLGPPTILRGNASEIMAVHQDEARTKGVDSAAASSTALAAARAIHQ
                     RHGCIVSISGETDYVVHEGGTLAIRNGHPMMPRVTGLGCTASALCGAFAAVERDAALA
                     TAAAMAVMGVAGEMAAEGCPGPGSFQVRFLDALYSLTEEDLRRRLRIAPENG"
     gene            complement(1191..2219)
                     /locus_tag="PLW16_07905"
     CDS             complement(1191..2219)
                     /locus_tag="PLW16_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70868.1"
                     /translation="MNAVSPDRNRGGRRPAWPVLLIHNLDAAWTEAEIAVALDEVRTL
                     EGALKEQGHPVANLTVRDRDLERLLEPFDPRAWVVFNNCEGLPGVPGSEARVIDLLDE
                     MGFAYTGATAAAMDLAWDKPRVKRLLDEYGLPTPRWRLCDTPDPAGWRIFPAIVKPAR
                     EHCSLGVTTDAVATEPGELSDRIAFVLDSYRQPALVEDFIDGREFHVSVWGDGEVLPA
                     AEMDFAAFAEVKDRLCTYESKFEPGSRHYEGIGLRLPADLSPEENEALRRTVVEGYRA
                     VGCRDYARLDLRLRDGVFYILDINPNPDISSETSMACAAEVAGFSYGAMLSRIVNFAA
                     RRHPVFGA"
     gene            complement(2242..2484)
                     /locus_tag="PLW16_07910"
     CDS             complement(2242..2484)
                     /locus_tag="PLW16_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70869.1"
                     /translation="MIDRLIAFLSRFRTDHRQLVAVLEDLITLVRDLDTQEKVRSALD
                     ILKREAVDMEESLSAFRIPSDAGEDKTNDGQRDVRT"
     gene            2869..4458
                     /gene="cimA"
                     /locus_tag="PLW16_07915"
     CDS             2869..4458
                     /gene="cimA"
                     /locus_tag="PLW16_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPB70870.1"
                     /translation="MNNTSDILLYDTTLRDGTQGEQINFSAEEKLRIARRLDDLGFHY
                     LEGGWPGSNPKDMRFFELAKGASFRNTRLTAFGSTKKVSGRPADCPQLQALLKAETPA
                     VTIFGKSWNLQVTEILGISRDENLEMITDTLSFLKAHGKEVLFDAEHFFDGFKADPGY
                     ACQTLRCAAEAGADVLVLCDTNGGTLPHEISEIVADVRRRFPETLLGIHAHNDGGLAV
                     ANTLAAVRQGARMVQGTVNGFGERCGNADLVTVIGNLQLKMGRSCLSDDALAQLTNLS
                     HYVTDVANVPLRSSHPFVGRSAFAHKGGVHVNAVAKNPAAYEHIDPARVGNRRRVLVS
                     DMSGKTNIAYKAREFGIDLGEDAGLNRRILETIKRMEDEGYQFDAADGSFSLLIRKLK
                     GEFRDPFALECFHVINGRTGDGPSHSQATIKISVGGVEELAAAEGNGPVNALDNALRK
                     ALKTFYPQVEEMHLVDFKVRIVEGSEGTAAKVKVLIESRDGTDVWSTIGVSENIIEAS
                     WQALADSIQYKLAKDRLNKNT"
     gene            4647..5258
                     /locus_tag="PLW16_07920"
     CDS             4647..5258
                     /locus_tag="PLW16_07920"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB70871.1"
                     /translation="MIVSINRQNPQMRLIRKAVDILRQGGIIVYPTDTFYGLGCDLFN
                     KRGIERIYDIKRRSRKQPFSFICADLKEISRYARVSDYAYKTMRRLLPGPYTFILEAS
                     RLVPKIILPKRLTTGIRVPDDAICLALVRELGQPIISTSVSDERGEILNDPQEIDKKF
                     RHCVDLVIDGGMLVAEPSSVISLVDDRVEVIRTGKGDVSAFLQ"
     gene            5398..7332
                     /locus_tag="PLW16_07925"
     CDS             5398..7332
                     /locus_tag="PLW16_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPB70872.1"
                     /translation="MKKEMLINAVHPEQKRMAILDDGKLVEFNIQMAVRDPINGNIYK
                     GIVQKVERGLQAAFVNYGGKKDGFLPLRDVNPEYFHDGKDNGRASLKVGQEVIVQVLR
                     EVGERKGALLTTYISLPGRYLVLLPNKPGGGISRKIEDEEDRRQLKELIGQIKIDEGF
                     GYIIRTAGMSRTKQELARDYQHLSRLWKEILKAAAELPAPSLIYQETDFGVRSLRDYF
                     TSDIEEILVDDLETFRKMRAYCRAVAPRNLKMIKHYKDKTPLFNRFRLEEQIGVIYQE
                     QANLKSGGYIVLNPTEAMITIDVNSGRGSNKRNVEETAFKTNMEAAEEIARQLRLRDL
                     GGLIAIDFIDMRDRKHEAEVEKAFKKALSLDRSRIQLSRISKFGILELSRQKKQSTIQ
                     EISYTSCPYCKGRGSRPSLEYTALSAFRKIESHAVQGQYASIRATLPHEISDYLLNQK
                     RQEIGKLEAAYDLSIVISGRADLAWDEIRIDTTRKETPSETDLEPAESGVISDQELWE
                     EEGDEREITAAPAPREPEAPNGTPVADTAPPPAKKKSRRRSRHRRKKTADKAVEAAVP
                     TETTETAAPAAPVNGEIRRIQLRETTMAEGEPGPETADAAASPPDPKPASEGVEMPAP
                     LTGYPLEPFAEDDADDGLPF"
     gene            complement(7339..8160)
                     /gene="proC"
                     /locus_tag="PLW16_07930"
     CDS             complement(7339..8160)
                     /gene="proC"
                     /locus_tag="PLW16_07930"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPB70873.1"
                     /translation="MLRDRRIGVIGGGKMGGALIDGIISRGLVQPSAVTVSDTLAERR
                     EELKARCGVTVTADNRIAVRDADLVILAVKPQNMAEVLADLAHDLTARPVIVSIAAGI
                     STAFIEGSCPAPLRVIRVMPNTPAMVGAGATALTRGRNATAEDLALGREIFAAVGMTV
                     EVEEGLMDVVTGLSGSGPAYVFLFIEALTEAGVGLGLAPETALGLASQTLLGAAKLCL
                     QGEKSPAELRAMVTSPGGTTLEGLKALNAGGFRETVVAAVAAATERSRELGKARR"
     gene            8347..9120
                     /locus_tag="PLW16_07935"
     CDS             8347..9120
                     /locus_tag="PLW16_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB70874.1"
                     /translation="MIHTREIGGVRISCWTSETDFTSDRRSLFFIHGSGGDHTAWNGQ
                     IASFRDRFNVVAVDLPGHGQSGGGGEETVEAYVAWVRDTLAAFKLPKPVLVGHSLGAA
                     ISLTMALNHGEEIAGIVPVGGGIRMPVNPAILAGLESNPGPILTMAAQIVVAKRNRER
                     LSHQLLESLSAVNPRVMRGDFLACDRLDLTGTVGRIAVPSLVICGAEDKMTPPALSQA
                     IAEKIPGAHLALIEGTGHMVMLEDPAAFNNALAGFLSAL"
     gene            9151..9450
                     /locus_tag="PLW16_07940"
     CDS             9151..9450
                     /locus_tag="PLW16_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPB70875.1"
                     /translation="MFVVGNFIAAIARILDLVLTLYLWIVVFRAVISWVNPDPYNPIV
                     RFLERATEPLLAPLRRWTPLRGMGIDLSPILLIAAIYFLQVFLVRTLMQLAGTLH"
     gene            9454..9807
                     /locus_tag="PLW16_07945"
     CDS             9454..9807
                     /locus_tag="PLW16_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPB70876.1"
                     /translation="MNAPRTLMVIHETSEGVTFPIRVQPRASRCECAGVQDGAVRLKI
                     TAPPVEGRANEECVRLLAETLKLRKRQITILTGLKSRNKVVAVAGLSAAALRERLADL
                     AAAGPDIPAPQESAT"
     gene            9804..11405
                     /gene="murJ"
                     /locus_tag="PLW16_07950"
     CDS             9804..11405
                     /gene="murJ"
                     /locus_tag="PLW16_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPB70877.1"
                     /translation="MNQTVPGGENRQVAHAAGVVGAATMLSRVFGFIRDMVVAGLFGA
                     GFKTDAFFVAFRIPNLLRRLLAEGSLTVAFVPVFTDYLKRKSRADALEFANVCFTLLS
                     IVLVVVSCLGIVFAPLIVTIMAPGFVHAPAQFDLAVFLTRFMFPYIFFISLVALCMGI
                     LNALRHFAAPALSPVVLNIAMILAALLLRDFFAEPIVALAVGVMIGGVLQLAMQWPFL
                     VRMGARLRLNFHFRHPGLKQMGILLIPTLIGSAVYQLNIFIGTILASLLPKGSVSYLY
                     YADRIVELPLGVFAIAVGTASLPSLSALVTRGELGEMKRTLSFSLGLILFITLPATVA
                     LIALRVPILSVLFQRGAFTAQSTLLTAQALLCYTLGLWAFSVIRVIDAAFFALQDRKS
                     PLKAAIVALLVNVAFSIVLMGPLKHGGIALATSIASAVNVIMLAVILRRKLGRILEAD
                     FFRSIAKMAAASLVMWASLRLVEAILPWRTDGPFDERLLFLTVSVAVGAAVFFVTAFL
                     VRSPEMVTFVGAVNRRLHRRPHPPQ"
     gene            11417..12172
                     /locus_tag="PLW16_07955"
     CDS             11417..12172
                     /locus_tag="PLW16_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPB70878.1"
                     /translation="MEENTPVAANEMTVETEASLAKYLAATPTIESGHPEVAAFAARH
                     AAGAGDPVEKAVRLYYAVRDGIRYNPYAFFLSVDGLKASQTLRAGQAWCVPKAILLAA
                     CCRAHGIPARLGYADIRNHLSTARMREAMRTDIFYWHGYTSLYLEGRWVRATPAFNIE
                     LCRKFNLLPLEFDGRADSLFHPFDAVGNRHMEYLHYRGEYADVPLDGLIATFRDLYPE
                     LWRTGEAGLGTDADFDRDVDAETTGAVAPGQKR"
     gene            12275..13552
                     /gene="serS"
                     /locus_tag="PLW16_07960"
     CDS             12275..13552
                     /gene="serS"
                     /locus_tag="PLW16_07960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPB70879.1"
                     /translation="MLDIKFLRQNLDQVRRKLAERGQTIDLEQFSALDARRRNLLQEV
                     ETLRNERNTVSKEIGDRKKRKADAADLIARMGEVSARIKNLDAILEETESALEAIVMT
                     IPNVPHDSVVRGTSSEDNPIVRTWGDLPRLAFPPRPHWELGESLGILDFTRGSKIAGA
                     RFTLYRGAGALLERAIINFMLDLHTGEHGYMEILPPFLANRESMTGTGQLPKFAEDLF
                     KIEGLDYYLIPTAEVPVTNIHREEILNERDLPIRYVAYSPCFRAEAGSYGKDTRGLIR
                     QHQFNKVEMVKFTTPETSYEELENLTANAEEVLRRLEIPFRTVSLCTADLGFSSAKTY
                     DVEAWMPGQDAYREISSCSNFEDFQARRAAIRFRREATGKVEFVHTLNGSGLAAGRTT
                     VAVLENYQRSDGSILIPKALRPYMGGREKIAPA"
BASE COUNT         2492 a         4609 c         4208 g         2335 t
ORIGIN      
        1 gtttcgacgg acacgccgat gatcgcccgg ggccccagca gcctccgggc cagggtatag
       61 ggcatgtcgt tctgaccgat atgcacgccg tcggccccca ccgccagggc cacgtccaga
      121 cggtcgttga tgatcagcgg aacgttgaaa ggggtgagca ggtcccggac ggccagcgcc
      181 tcctccacga actgccgagt cgaaagcgtc ttctcccgca gctggacgca ccgcgccccg
      241 ccgcggacgg acgccaggac cacgtccgtc agagaccgcc ggccgcacag gtcgcggtcc
      301 gtcaccaggt acaatccttc catgattctt cccctccgtc ggcgcctacc cgttttccgg
      361 cgcgattctc aagcgccgcc ggaggtcctc ttccgtcagg ctgtacaggg cgtcgagaaa
      421 gcgcacctgg aacgatcccg gccccgggca gccctccgcc gccatttctc ccgcaacgcc
      481 catcacggcc atcgcggccg ccgtcgccag ggcggcgtcc ctctccacgg cggcaaaggc
      541 gccgcacagg gccgacgcgg tacagcccag gcccgtcacc cggggcatca tcgggtgacc
      601 gttccggatg gccagggttc caccttcgtg aaccacatag tccgtttcgc cgctgatgga
      661 cacgatgcaa ccgtgacgct gatgaatcgc ccgggccgcg gccagggccg tggaggaggc
      721 cgcggcgctg tccacaccct tcgtccgggc ctcgtcctga tggaccgcca tgatctccga
      781 ggcgttcccc cggagaatcg tcggcggccc caacgagatg agctcccgga ccgtctgcgt
      841 ccggtaggcc gtggcacccg cgccgacggg gtccaggacg acgggaatgt gccgggcgcg
      901 ggccgccggg agggcgagac gcatggcccg gatccagggg tcgctcaggg tcccgatgtt
      961 gacgacgagg gcgccggcga gcaccgccat ctcggacgct tcattctccg catgggccat
     1021 gaccggagag gccccgaggg ccagcagggc gttggcggtg ttgttcatga ccacgtaatt
     1081 ggtgatattg tggaccaggg gggcctgccg gcgaatggcc gtcacggcct cccagatcga
     1141 tgttgccgtg ggttccatgg atgcactccg ctcctttcaa ctggaaaaat tcacgcaccg
     1201 aagaccgggt ggcgtcgggc ggcaaaattc acgatccggc tgagcatcgc cccgtaggag
     1261 aagccggcga cctcggcggc gcaggccata ctggtttccg agctgatgtc ggggttgggg
     1321 ttgatgtcca ggatataaaa gacgccgtcc cggagccgga ggtccagacg ggcgtaatcc
     1381 cggcagccga cagccctgta gccttcgacg acggtccgcc gcagggcttc attctcctcc
     1441 ggagacagat ccgccggcag cctcaggccg attccctcgt aatgccgcga accgggttca
     1501 aacttcgact cgtaggtgca gagccgatcc ttcacctccg cgaaggccgc aaagtccatc
     1561 tccgccgccg gcagcacctc gccgtcgccc caaacggaga cgtgaaattc ccgcccgtcg
     1621 atgaagtcct ccaccagggc cggctggcgg tatgaatcca gaacgaaggc gatccggtcc
     1681 gacagttccc ccggctccgt cgccacggcg tcggtcgtca cccccaggct gcaatgctcc
     1741 cgggccggct tgacgatcgc cgggaagatc cgccagccgg ccggatcggg ggtatcgcag
     1801 agccgccagc ggggcgtcgg cagaccgtat tcgtcgagca accgcttcac ccgcggcttg
     1861 tcccaggcca ggtccatcgc cgccgccgtc gcgccggtgt aggcaaagcc catctcgtcc
     1921 agaaggtcga tcacccgggc ctcgctgccg gggacgcccg gaagaccttc gcagttgttg
     1981 aaaacgaccc acgcccgggg atcgaacggc tccagaagcc gctccagatc acgatcgcgg
     2041 accgtcagat tggcgacggg gtgcccctgc tccttcaggg caccctccag cgtccgcact
     2101 tcgtcaaggg cgacggcgat ctccgcttcg gtccaggcgg cgtccagatt gtggatcagc
     2161 aggaccggcc aggccgggcg ccgccccccc cggttccgat ccggcgaaac cgcgttcatg
     2221 gcgtcccgca ttgtttcccg gtcatgttcg tacgtcccgt tgtccgtcgt tcgtcttgtc
     2281 ctccccggcg tcggaaggaa tccggaaagc ggagagggat tcctccatgt ccacggcctc
     2341 ccgcttcagg atatccaggg ctgaccgcac cttctcttgc gtatcgaggt cccggaccag
     2401 ggtgatgagg tcctccagga cggcgacgag ttgccgatgg tcggtccgaa accgggacaa
     2461 gaaggcgatg agccgatcga tcatggtgtt gatctgttcg gaaacacggc ctcatgtccc
     2521 acttgcatac gcgctccctc gcaccggtcc gggtgcggcg attctcatcg tccggaaatg
     2581 gaaattttcc gggcacgatc atcttttaaa acaccggttt tgtcaagcgc ggagatggcc
     2641 agatttctgt tgacaaaggc cgtcaaaccc ggtatgagag cggtcattac agacacggtg
     2701 cggcaggaat gatgaccccc cttaccatcg ccattcggat tgaaggcata attaggcccc
     2761 ccgccatagg agacctgata atggtatagc cccgtccgca agcctcatcg aacttcagcc
     2821 gttatcaggt cactgatagc ggctttttta tttttgtgcg agggaatcat gaacaacacg
     2881 tccgatatcc tgctttacga caccaccctg agagacggaa cccagggaga acagatcaac
     2941 ttctccgcgg aggaaaaact gcggatcgcc cggcgactcg acgatctggg gtttcactat
     3001 ctcgaaggcg gctggcccgg atccaatccg aaggacatgc gcttcttcga attggcgaaa
     3061 ggcgcctcct tccggaatac ccggctgacg gccttcggca gcacaaagaa ggtctcgggc
     3121 cggccggcag actgccccca actgcaggcc ctgctcaagg ccgaaacccc ggccgtcacg
     3181 atcttcggca aatcctggaa cctgcaggtc acggagattc tcgggatctc gcgtgacgaa
     3241 aatctggaga tgatcacgga taccctctct tttctcaagg cccacggcaa ggaggtcctc
     3301 ttcgacgcgg agcacttctt cgacgggttc aaggcggatc ccggctacgc ctgccaaacc
     3361 cttcggtgcg ccgctgaagc gggcgcggac gtcctcgttc tgtgcgacac caatgggggg
     3421 accctgccgc acgagatcag cgagatcgtc gcggatgtcc ggcgccgttt cccggaaacg
     3481 ctcctgggga ttcatgccca caacgacggc ggcctggccg tcgccaacac cctggcagcg
     3541 gtgcggcagg gggcccggat ggttcaggga accgtcaacg gattcggcga acggtgcggc
     3601 aacgccgacc tcgtcaccgt catcggaaac ctgcaactga agatgggccg gtcctgtctc
     3661 agcgacgacg ccctcgccca gttgacgaac ctctcgcact acgtcaccga cgttgcgaac
     3721 gttcccttgc ggagttccca ccccttcgtc ggccgcagcg ccttcgccca caagggcggc
     3781 gtccacgtca atgccgtcgc caagaatccc gcggcctatg aacacatcga tccggcgagg
     3841 gtgggcaacc ggcgacgcgt cctcgtctcc gacatgtccg ggaaaaccaa catcgcctac
     3901 aaggcgcggg agttcggcat cgacctcggc gaggatgccg ggctcaaccg gcgcatcctg
     3961 gagacgatca aacgcatgga ggacgagggg taccagttcg acgccgccga cggctccttt
     4021 tccctgctca tccgcaagct caagggcgaa ttccgggatc cctttgccct cgagtgcttc
     4081 cacgtcatca acggccgcac cggagacggg ccttcccatt cccaggccac gatcaagatc
     4141 tccgtgggcg gcgtggagga actcgccgcg gcggagggca acggccccgt gaacgccctg
     4201 gacaacgccc tgcgaaaggc cctcaagacc ttctacccac aggtcgagga gatgcacctg
     4261 gtcgatttca aggtccggat cgtcgagggc agcgagggca ccgccgcgaa ggtcaaggtg
     4321 ctcatcgaat cccgcgacgg cacggacgtc tggagtacca tcggcgtgtc cgaaaacatc
     4381 atcgaggcga gctggcaggc cctcgcggac agcatccagt acaaactggc caaggaccgc
     4441 ctcaacaaga acacctaaaa aggcctgtcc cccgatcggg agaggggggg gccgctccgc
     4501 tcccccttcc ccggaggctc atttgtttgt cccgctccca cccctgcaag atggatattg
     4561 attatccccc atatttctga tacataggcc gcgaaaagga atggggaagg ccgctatgat
     4621 ccctctccgt gcggttggga tgttccatga ttgtctcgat caacaggcaa aacccacaga
     4681 tgcgcttgat ccgcaaggcc gtcgatatcc tgcgtcaggg gggaatcatc gtctatccca
     4741 cggacacctt ctacggcctg ggatgcgacc tcttcaacaa acgggggatc gaaagaatct
     4801 acgacatcaa gcggcgcagc cgaaagcagc ccttcagttt catctgcgcc gacctcaagg
     4861 agatcagccg ctacgcccgc gtttcggact atgcctacaa gacgatgcgg cgcctgctgc
     4921 ccggccccta caccttcatt ctcgaggcct cccggctcgt tcccaaaatc atccttccca
     4981 agcgactgac gacggggatc cgcgtccccg acgatgcgat ctgtctggcc ctggtgcggg
     5041 aattgggaca gccgatcatc agcacgagcg tttcggacga acgcggggag attctcaatg
     5101 acccccagga gatcgataag aaattcagac actgtgtcga cctggtcatc gacggcggga
     5161 tgctggttgc cgagccgtcc agcgtcatca gccttgtgga cgaccgggtc gaagtgatca
     5221 gaacgggaaa aggcgacgtc tccgcctttt tacagtaaac aggcaaggtt catgcgcccc
     5281 ggatcctgag gggccgcggg cgggcgatcc actgggagag ggcagcggtc gcccccttac
     5341 gtacccgcgg acacgggcac gcccgggcgc gctggccgaa ttgccggaag gatctttatg
     5401 aaaaaggaaa tgctgatcaa tgccgtgcat ccggagcaga aacggatggc catcctggat
     5461 gacggcaaac tggtggagtt caacatccag atggccgtcc gcgaccccat caacggcaat
     5521 atctacaagg gaatcgtgca gaaagtggag cggggactcc aggctgcctt cgtgaattac
     5581 ggcggcaaga aggacgggtt ccttcccctg cgcgacgtaa atccggagta ttttcacgac
     5641 ggcaaggaca acggccgcgc ctccctgaaa gtcggtcagg aagtgatcgt ccaggttctc
     5701 agggaggtgg gggagcgcaa aggggcgctc ctcaccacct acatctccct gccgggacgg
     5761 tatctcgtcc tgctgcccaa caaacccggg gggggcattt cgcgcaagat cgaagacgag
     5821 gaagaccgcc ggcagctcaa ggaactcatc ggccaaatca agatcgacga aggcttcggc
     5881 tacatcatcc gcaccgcggg catgagccgg accaagcagg aactggcccg ggactaccag
     5941 cacctctccc gcctgtggaa agagatcctg aaggccgccg cagaattgcc tgccccctcg
     6001 ctgatctacc aggagaccga tttcggcgtc cgctcgctga gggattactt cacctcggac
     6061 atcgaggaaa tccttgtgga cgatctcgaa accttccgga agatgcgggc ctactgccgg
     6121 gccgtggcgc cccgcaacct gaagatgatc aagcactaca aggacaagac gccgctgttc
     6181 aaccgcttcc gcctggaaga gcagatcggc gtcatctacc aggagcaggc caacctcaag
     6241 tccggcggtt atatcgtgct caaccccacc gaggccatga tcacgatcga cgtcaattcc
     6301 ggccgcggct cgaacaagcg caatgtcgag gaaacggcct tcaagaccaa catggaggcc
     6361 gccgaggaaa tcgcccgcca gctccgcctg cgcgacctgg gagggctcat cgccatcgac
     6421 ttcatcgata tgcgggaccg caagcacgag gcggaggtgg aaaaggcgtt caagaaggcc
     6481 ctgagcctcg accggtcgcg cattcagctg tcccggattt ccaaattcgg catcctggag
     6541 ctgtcccggc agaaaaagca gtccaccatc caggaaatca gctatacgag ctgcccctac
     6601 tgtaaaggcc ggggatcgcg gccctccctc gagtacaccg ccctgagcgc cttccggaag
     6661 atcgagtccc acgccgttca ggggcagtac gcgtcgatcc gtgccacgct gccccacgag
     6721 atttccgatt atctcctcaa ccagaagcgc caggaaatcg gcaagctcga agccgcatac
     6781 gacctcagca tcgtgatttc cgggcgggcg gatctcgcct gggacgagat ccggatcgac
     6841 accacgcgga aggaaacgcc gtcagagacc gacctcgaac cggcggagag cggggtcatc
     6901 agcgatcagg aactgtggga ggaagaaggg gacgaacggg agatcaccgc cgccccggcc
     6961 ccgcgggagc cggaagcacc gaacggaacg ccggtcgcgg ataccgcgcc gccgcccgcc
     7021 aagaaaaagt cgcggcgacg ttcccgtcac cggaggaaaa aaacggccga caaggccgtg
     7081 gaggccgcgg tgccgacaga gacgacagag acggcggcgc cggcggcgcc cgtgaacgga
     7141 gaaatccgac ggatccagtt gcgggaaacg acgatggcgg agggggagcc gggaccggag
     7201 acggcggacg ccgctgcttc tccacccgac ccgaagccgg cgtcggaggg ggtggagatg
     7261 cccgcgcccc tcaccggata tccgttggaa cccttcgccg aagacgacgc ggacgacggg
     7321 cttccgtttt agggcctctc agcgccgggc cttccccagt tcccgggaac gttccgtcgc
     7381 cgccgcgacg gctgccacga cggtttcccg gaagcccccg gcattcaggg ccttcaggcc
     7441 ttcgagggtg gttccgcccg gagaggtcac catggccctc agctccgccg gcgacttttc
     7501 gccctggagg cagagcttcg ccgcccccag cagggtctgg gaggcgagcc ccagcgccgt
     7561 ttccggggcc agtcccaggc cgacaccggc ctccgtcagg gcctcgatga acagaaagac
     7621 gtaggccgga ccgctgccgc tcaatcccgt cacgacgtcc atcaggcctt cctccacctc
     7681 gaccgtcatc ccaacggcag cgaagatttc ccgacccagg gcgagatctt ccgcggtggc
     7741 gttcctcccc cgcgtcaggg cagtcgcccc ggcgccgacc atggccgggg tgttgggcat
     7801 cacccggatg acgcggagcg gcgccggaca ggatccctcg atgaaggccg ttgaaatccc
     7861 cgcggcgatc gaaacgatca ccggccgcgc ggtgaggtcg tgggctaggt cggccagcac
     7921 ctcggccatg ttctgcggct tcaccgccag gatcaccaga tcggcgtccc ggacggcgat
     7981 ccggttatcc gcggtcaccg tcacgccgca gcgcgccttc agctcttccc gcctttccgc
     8041 cagggtgtcg ctcaccgtca cggccgacgg ttgaaccagg ccgcgggaga ttattccgtc
     8101 gatcagggcg ccgcccatct ttccgccgcc gatgacgccg attcttcggt ctcgtaacat
     8161 cgcgctccat ccctccggat tctgcagttt gaatcgtccc tggggatggc tgttaataca
     8221 aaagcgccgg cggcgtcaat ctttgtttgc acgcaaagtg cgatctgtgg tagtgctgac
     8281 cgtatccggc cgcgcctctc gatcggtgcc ggcgcaggcc gcaacactct gtcccgggag
     8341 ggcgacatga ttcatacgcg ggagatcggc ggcgtcagga tctcgtgctg gaccagcgag
     8401 acggatttca ccagcgaccg ccggagcctc tttttcatcc acggctcggg cggggatcac
     8461 acggcctgga acgggcagat cgcctccttc cgggaccggt tcaacgtcgt cgccgtggac
     8521 ctgcccggac atgggcaatc cgggggaggc ggcgaagaga ccgtcgaggc ctacgtggcc
     8581 tgggtgcggg acacccttgc cgccttcaaa ctgcccaagc cggttctcgt cggtcattcc
     8641 ctgggtgcgg ccatcagcct caccatggcc ctgaaccacg gcgaggagat cgccggcatc
     8701 gtgcccgtcg gaggcgggat ccggatgccg gtcaatccgg ccatcctcgc ggggctggaa
     8761 tccaaccccg ggccgatcct gacgatggcc gcccagatcg tcgtggcgaa gcggaaccgg
     8821 gagcggctct cgcaccagct gctcgagagc ctgtccgccg tcaacccccg ggtcatgcgc
     8881 ggcgattttc tcgcctgcga ccgcctcgac ctgaccggga ccgtcgggcg catcgccgtg
     8941 ccgtcgctcg tgatctgcgg cgccgaagac aagatgaccc cgcccgccct ttcgcaggcg
     9001 atcgcggaga agatcccggg ggcgcatctg gcgctcatcg aagggacggg acacatggtc
     9061 atgttggagg atccggcggc cttcaacaac gcgctggcgg gctttctgtc cgccctgtag
     9121 gaaaactccg catttgctcg agaggcaaac atgttcgttg tcggcaattt catcgcagcc
     9181 atcgcccgga tcctcgatct cgtcctgaca ctctatctgt ggatcgtcgt tttccgggcg
     9241 gtcatctcct gggtcaatcc cgacccgtac aatccgatcg tccgctttct cgagcgcgcg
     9301 acggagccgc tcctggcccc tctccggcgc tggaccccgc tgcggggaat gggcatcgac
     9361 ctctccccga tcctgctgat cgcggcgatt tatttcctgc aggtctttct cgtcaggaca
     9421 ttgatgcaac tggccggcac gctccattga cggatgaacg cgccccgcac cctcatggtc
     9481 attcacgaaa cctccgaagg cgtcaccttt ccgatccggg tccagccgcg ggcgtcccgg
     9541 tgtgagtgcg cgggcgtgca ggacggggcc gtccggctga agatcaccgc cccacccgtc
     9601 gaagggcggg ccaacgagga gtgtgtccgg cttctcgccg agacgctgaa gctccgcaag
     9661 cggcagatca cgatcctgac cggcctgaag tcccggaaca aggtcgtcgc cgtagccggg
     9721 ctgagtgcgg cggcgcttcg tgaaaggctg gcggatctcg ccgcagccgg ccccgatatc
     9781 ccggcgccgc aagagagcgc gacatgaacc aaaccgtccc gggcggagag aacaggcagg
     9841 tggcccacgc ggcgggcgtg gtcggggcgg ccacgatgct ctcccgggtc ttcggtttca
     9901 tccgggacat ggtcgtcgcc ggccttttcg gcgcggggtt caagaccgac gccttcttcg
     9961 tcgccttccg gatccccaac ctcctccggc gcctgctcgc ggaagggtcg ctgaccgtcg
    10021 ccttcgttcc cgtcttcacg gattacctga aaaggaagtc ccgcgctgat gccctcgaat
    10081 tcgccaacgt ctgcttcacc ctcctgtcca tcgtcctcgt cgtcgtttcc tgcctaggaa
    10141 tcgtcttcgc cccgctgatc gtcacgatca tggcccccgg gttcgtccac gcgccggcgc
    10201 agttcgacct cgccgtcttc ctgacgcggt tcatgttccc ctacatcttc ttcatctcgc
    10261 tggtcgccct gtgcatgggc atcctgaacg ccctgcgcca cttcgccgcg ccggccctgt
    10321 cgccggtcgt cctcaacatc gccatgatcc tggccgcgct gctcctgcgg gatttcttcg
    10381 cggagccgat cgttgccctg gctgtggggg tcatgatcgg cggcgtcctg cagcttgcca
    10441 tgcagtggcc gtttctcgtc cggatgggcg cgcgccttcg cctgaacttt cacttccggc
    10501 acccggggct gaaacagatg ggaatcctcc tgatccccac gctcatcggt tcggccgtgt
    10561 atcagctcaa catcttcatc ggcaccatcc tggcgtcgct cctgcccaag ggcagcgtct
    10621 cctatctcta ctacgccgac cggatcgtcg agctgccgct cggggtcttc gccatcgccg
    10681 tgggcacggc ctccctgccc agcctctccg ccctcgtcac gcggggagag ctcggcgaga
    10741 tgaaacgaac gctctcgttt tccctcgggc tgatcctctt catcaccctt ccggccacgg
    10801 tcgccctgat tgcgctccgg gtcccgatcc tctccgtcct cttccagcgg ggcgccttta
    10861 cggcccagtc gaccctcctg accgcccagg cgctcctctg ttacaccctc ggcctgtggg
    10921 cgttctccgt cattcgggtg atcgacgccg ccttcttcgc cctgcaggac cgcaaatcac
    10981 cgctcaaggc cgccatcgtg gccctgctcg tcaacgtcgc gttcagcatc gtcctcatgg
    11041 gccccctgaa gcacggcggc atcgccctgg ctacctccat cgcctcggcg gtcaacgtaa
    11101 tcatgctcgc cgtcatcctg aggcgcaagc tcggccggat cctggaggcg gatttcttcc
    11161 ggtcgatcgc caagatggcc gcggcgtcgc tcgtcatgtg ggcctccctc cggctggtgg
    11221 aggcgattct accctggagg accgacggcc cgttcgacga acgtctcctg ttcctcacgg
    11281 tctcggtagc cgtgggcgcg gcggtctttt tcgtcaccgc cttcctcgtg cgcagcccgg
    11341 agatggtgac cttcgtcggc gccgtgaacc gaagactgca ccgccggccg caccccccgc
    11401 aatgaaagga aaccccatgg aagaaaacac acccgtcgcc gcaaacgaga tgaccgtcga
    11461 gaccgaggcg agtctcgcca aatatctggc cgccacgccc acgatcgaga gcggccatcc
    11521 ggaggtggcc gccttcgccg cgcggcacgc cgccggggcg ggagatcccg tggaaaaggc
    11581 ggttcgtctc tactacgcgg tacgcgacgg catccgctac aacccctacg ccttctttct
    11641 gtccgtggac ggcctcaagg ccagccaaac cctgcgggcc ggccaggcct ggtgcgtccc
    11701 gaaggccatt ctgctggcgg cctgctgccg ggcgcacggg atcccggctc ggctcggcta
    11761 tgcggacatc cggaaccacc tctccacggc ccgcatgcgc gaggccatgc ggaccgacat
    11821 cttctactgg cacgggtaca cctcgctcta cctggagggg cgctgggtcc gggccacgcc
    11881 ggccttcaat atcgagctct gccggaagtt caacctcctc ccattggagt tcgacgggcg
    11941 ggcggactcg ctcttccatc ccttcgacgc ggtgggaaac cgccacatgg aataccttca
    12001 ttaccggggc gaatatgccg atgtcccgct cgacggcctg atcgccacct tccgggacct
    12061 ctacccggaa ctgtggcgga cgggagaagc gggattgggc acggacgcgg acttcgaccg
    12121 cgatgtggac gccgaaacca cgggggccgt cgcgcccggt caaaagcgtt gacttcaaat
    12181 cgctaaagtg gtaggaaacc tctccctccg aaggcccgga tacggatccc ggcatcggag
    12241 ggagaatcgt tagcggccgg cggtacagcg ctttatgctc gacatcaagt ttttaaggca
    12301 gaatctcgat caggtccgca ggaaactggc ggagcgcggc cagacgattg acctggagca
    12361 attttccgcc ttggatgccc ggcgacgcaa cctcctccag gaagtggaaa cgctgcgcaa
    12421 cgagcgcaac acggtttcca aggagatcgg cgaccggaag aagcgcaagg ccgatgccgc
    12481 cgatctgatc gcgcggatgg gagaggtttc cgcgcggatc aagaacctcg acgcgatcct
    12541 ggaagagacc gagtcggcgc tggaggcgat cgtgatgacg atccccaacg tgccgcacga
    12601 ctcggttgtc cgcggaacga gttccgagga caacccgatt gtccggacct ggggcgacct
    12661 cccccggctc gcgttccccc ccaggccgca ctgggagctc ggggagagcc tcggcatcct
    12721 ggatttcacc cgggggtcga agatcgccgg cgcccggttc accctgtatc ggggggcggg
    12781 cgccctgctc gagcgggcca tcatcaactt catgctggac ctgcacaccg gcgagcacgg
    12841 ctatatggaa atcctgccgc ctttcctggc gaaccgggaa agcatgacgg ggacgggaca
    12901 gcttcccaag ttcgccgagg atctcttcaa gatcgagggg ctggactact acctgatccc
    12961 cacggcggag gtgccggtca cgaacatcca ccgggaggag atcctgaacg agcgggacct
    13021 gccgatccgt tacgtcgcct attcgccctg ctttcgggcg gaggcgggct cctacggcaa
    13081 ggacacgcgg gggctgatcc ggcagcacca gttcaacaag gtggagatgg tgaagttcac
    13141 cacgccggag acctcgtacg aggagctgga aaatctgacg gccaacgccg aagaggtcct
    13201 gcggcggctt gagatcccct tccggacggt cagcctctgc acggcggacc tgggcttctc
    13261 ctccgcgaag acctacgacg tggaggcttg gatgcccggc caggacgcct accgggagat
    13321 ctcctcgtgc agcaacttcg aggacttcca ggcccggcgg gcggccatcc gcttccggcg
    13381 cgaagccacg ggcaaggtgg aattcgtcca caccctgaac gggtcgggtc tggccgcggg
    13441 gcggaccacc gtcgccgttt tggagaatta tcagcggtcc gacgggagca tcctcattcc
    13501 gaaggcgctg cggccctaca tgggcggccg ggaaaagatc gccccggcgt gatcgatcgt
    13561 tgaaagagga gggatggccg agtggtcgaa ggcggcggtc ttgaaaaccg ttgaccgaaa
    13621 ggttcgcggg ttcgaatcct gctc
//
LOCUS       DAOOLJ010000050        13433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5047_length_13433_cov_17.864554, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000050 DAOOLJ010000000
VERSION     DAOOLJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13433
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5047_length_13433_cov_17.864554"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLW16_07965"
     CDS             complement(<1..260)
                     /locus_tag="PLW16_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPB70880.1"
                     /translation="MRSDAFRNISLQQLEALVFLVQEGNFSRAARRMNLSQPSLTKHI
                     QNMEGVLAAPVAIREKAGVTLTPEGRVVYDYARRVFKLREDAR"
     gene            complement(257..787)
                     /locus_tag="PLW16_07970"
     CDS             complement(257..787)
                     /locus_tag="PLW16_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB70881.1"
                     /translation="MQKIKKGEGMSRQDKEIKDFKILEAIMAAAPVCRLALAEDGVSY
                     IVPMHFAYRDRVLHFHTGRKGRKIDLLRKNDRVCFEMDILDEIVAAGEACRWTTRYRS
                     VIGYGRACLIDDSPEVEKSLDLLMEKYGGAGPWTYAARSLHQTLILRVAIASVTGKCA
                     PPLSQRDGGNAGEPAP"
     gene            996..2336
                     /locus_tag="PLW16_07975"
     CDS             996..2336
                     /locus_tag="PLW16_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPB70882.1"
                     /translation="MDRNPDAMELWRQERPLVIPCPRGAAPGLAAEVAGLGFPVRAET
                     DAAVETAGTFDDTLKLNLHLRTAQRVLFLLQAFRATTPGELYGGLLDLPWEAILPDDG
                     YVSVTSAVDHPTIRDSQFANVKCKDAIVDRIQKVRGRRPDSGPERTRSVVHLYWRGKD
                     ARIYLDTSGEPLSRRGYRLIPLDAPLQETLAAALVRATAWNGTDPFVNPMCGSGTLAI
                     EATLIARRIAPGLGRSNFGFMHVRGYRAAAWQALREAARLEVLAKPEGRIVATDIRPE
                     AVEAARRNARAAGVDRSITFTVCPFEHTPIPPGRGTLILNPPYGERMGELAALGDTYR
                     AIGDFLKHKAPGCQGFVFTGNPGLAKQVGLRTRQRLVFYNGGIECRLLHYDLYAGSRK
                     VREGSAVPNADGEESRTQRPLERARPAPAPSVGRGPAPKTRWIETHAKKRNPRR"
     gene            2308..3132
                     /gene="murI"
                     /locus_tag="PLW16_07980"
     CDS             2308..3132
                     /gene="murI"
                     /locus_tag="PLW16_07980"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPB70883.1"
                     /translation="MQKKETPGGNARHPIGVFDSGVGGLTVVRALMERLPFENLIYFG
                     DTARVPYGVKSAETINLYAAQITEFLLQREVKLLIIACNTMAAVAHEAVVSRSPVPVL
                     DVIDAGARMTIRTTRTKHVGVIGTPATVNSNTYARAIHRLDPEVRIFSQACALFVPLV
                     EEGWFDHPVTRLTAQEYLKPILAEGIDTLVLGCTHYPLLKALLREVTGPAILLVDSAE
                     AMADITADLLTARELANPDREPPDYRFYVTDVPLHFQGIGERILGRSLPHVERVSL"
     gene            3134..4309
                     /locus_tag="PLW16_07985"
     CDS             3134..4309
                     /locus_tag="PLW16_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70884.1"
                     /translation="MDRRTFLYSLLVTFFINAFLTLFFLFPAALQRQAMPLSLIGWVM
                     GVGSVGALLSRPIGSTMTETLGLIRSLLVLCLGLIVVSIPLIWARTFWPLFFLRAGGG
                     FLFGMAVVTLTTYQAFAIPVKQRGSTFAWIGIAYVAPQLVVLPLAEWFVTRGRIDLYM
                     GLAPLFALLTFLFALALPPPPAAADKDRSPAAPQETPWGSWADILVIPGFWALVLATF
                     TFAMLNGTTLQYLPAFIKDQGLIASSFLVVNSATALTVRLFAYRLMDRLNRPRAIGLI
                     LTGMGTALFLLHWADSHAAFIAGGLLYGMFMGIGFPIILALAPDIFPPRLMPKGVSIS
                     MLAMDAGFVLAPIFAGYLGQRFGLGLVFTAVGAGAVLSGLAVTLANRRIIRGGFWQS"
     gene            4549..5391
                     /locus_tag="PLW16_07990"
     CDS             4549..5391
                     /locus_tag="PLW16_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB70885.1"
                     /translation="MRIEEFKEIVYGKEDSGIVTVTLNIPKRKNAISPYTLYELFWAV
                     DAMEKDETARVMILTGAKDPDNDDPAKEAFCSGGYFNPNAMAGVSDEIKADIDMTDIA
                     QARLTLKMWQCDKPIIAAVNGLFIGGGFTMCLAGCDLIYCSEHAWAQLPFIGLGIIPE
                     LASSYLLPRLIGFQRAKEIMFFGDRIPAKKLFEMGIVNKVLPHDQLLAHARDMALRLI
                     PPKAAGYAVRTAKRTLHQPLFDAIATALNLENKGLNDCFKTTDFMEALTARVEKRPPA
                     FQGK"
     gene            5453..5887
                     /locus_tag="PLW16_07995"
     CDS             5453..5887
                     /locus_tag="PLW16_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPB70886.1"
                     /translation="MQNQRAGNTHVLRLDRGEEIVTELTRFCKSRGIALGLVTGIGAV
                     DRATIGLFRPAEKRYEATEMTGDHEILNLAGNVTTMNGEVYLHLHVTLGDAAHRAWGG
                     HLNAAFVSATAEIFVTVLEGACERAMDETVGLNLMRFGSPTS"
     gene            5991..7370
                     /locus_tag="PLW16_08000"
     CDS             5991..7370
                     /locus_tag="PLW16_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB70887.1"
                     /translation="MRRATLVTATVTAFLTPFMGSAVNVALPSIGRELAMDAIDLGWV
                     ATAFILAAAASLVPFGRIADIHGRKRIFMWGVVLFTAASGLCALAASARLLILARVIQ
                     GVGGAMIFGTGPAILVSVFPAAQRGRVLGFNVAAVYVGLSIGPFLGGLLTQHLGWRSI
                     FLVMVPLGCLLVYYILRHMPVEWADARGEPFDTVGALLYAFGLTALMYGFSCLPEMRG
                     AGFIAAGGAGLVLFAAWEVRTAYPILNIRIFRSNRTFAFSNLAALINYSATFAVSFLL
                     SLYLQYINGFTPVQAGVVLVATPVIQALLSPLAGRLSDRIAPRLLASGGMAATVVSLV
                     MFTRLDATSTLPYILTGLVILGGGFAFFSSPNMNAIMSAVDRSLYGVASGVSGAMRLI
                     GQMMSMGIAMIAFAMTMGCTRLSPDCYPLFLRALTIIFAILSVLCTAGVFASLARGPR
                     ARTDAAAGP"
     gene            complement(7462..7887)
                     /locus_tag="PLW16_08005"
     CDS             complement(7462..7887)
                     /locus_tag="PLW16_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70888.1"
                     /translation="MKKMLFVIVTIFIAVSAQAADVGVSVTIGQPGFYGHIDVGNFPK
                     PELVFPRPVVIAPVPVSVAPPPPVYMHVPPGHEKNWKKYCHKYKACDRPVYFVRNQWY
                     DNVYVPEYKARHARKGGKHGDDRGQRGGKAKGPGKGKRN"
     gene            complement(8269..10533)
                     /locus_tag="PLW16_08010"
     CDS             complement(8269..10533)
                     /locus_tag="PLW16_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70889.1"
                     /translation="MTDVHHDIAELHSISSTFKNLVRKLDQTTDELRQKHSQLLTIQD
                     ILEITRAEKDFDRILGLLLDKSMFITGAQTGSVLRLEGASIPQRKLALLQSGRESQVY
                     NEKLYKLRVVAEKGHKEPLEKDAVIDWEDYVAKDAVTEGKTLLVENIEQDERTRKAND
                     PAYGTPSFLSLPIFVENRLVAVVNLSNKREGRIFDSGDQRMLSIMLGEVGFALENALT
                     RANLDTQFQKIKKHNVELEKEIEARERVERALRESERKYRELLEFLPISVFEVDAKGM
                     LNTINRTCFETFGFTQEDIEKGLNAEDLFAPDDGHKVENHLTGIQEGRKIENVEFTMI
                     RKDGSPLPVLMSAAPVTRLNTVVGYRGALIDYTERRRAKQALEESEAKYRLLADKMTD
                     IVWIMDLNLRTLYVSPSIEKLFGFTPEERMRQDASEHITPESLRTVSEKLAEEFRLEA
                     QGGADLDRTVTLEVEHYHKDGSTRWVENIISGIRNDQGVLTGLHGVSRDITERKRAQR
                     DFQESWDQLVRAEKLAAVGQLSAGVAHEILNPLNIIGMELQIMQSMENLSADAEEELN
                     ICVSQVKRIAATAESLKQVTRVSDKKISIEDINDVIAGVLHLYVTPFTIDMIDTEVSY
                     RNDLPKIAMDRGKIEQVMMNLISNATLALEGKTDKRLKISTGLERTADGRDQLLIVVA
                     DNGRGIQGEDLNNIFDPFFTTRAQKKGPGLGLSISYGIVKDHGGVIRVENNDWGGATF
                     YISLPLLTTLGGND"
     gene            10498..10992
                     /locus_tag="PLW16_08015"
     CDS             10498..10992
                     /locus_tag="PLW16_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70890.1"
                     /translation="MKLGDVVMNVRHRPLLRHRFLQGFGNTGNFIEDLPNDEEPPQHK
                     GNNHAQQDEMVVFESVSVLVKDKRQHQQRDGQDERFDQQKSVSYSLPIRIQWIHFDET
                     FLSAYAKTFHARWGKAVCLVAAAFLQVGLTRGDVRPDNLLEGYPGADDGRHSPHAWDC
                     FGSG"
     gene            11316..11513
                     /locus_tag="PLW16_08020"
     CDS             11316..11513
                     /locus_tag="PLW16_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70891.1"
                     /translation="MNGYGKRWPGLLMALVVFLPGLARAADDALDNAVALSDGYMSLY
                     RIVAPNDRVVIYANDRFEPCP"
     gene            11534..13021
                     /locus_tag="PLW16_08025"
     CDS             11534..13021
                     /locus_tag="PLW16_08025"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPB70892.1"
                     /translation="MEIRLAGYNLDCDIIDACKKYFPDREDWTPETISAAYARISRSP
                     KTVTELRADARAEVEKARRSNENIVFAMGHSSVAEHAVFNLDVLGVSRLLVEEIERFR
                     LCSYTEKSQRYVLFRDDFVLPEEVRRAGLADAFTETIRAQYACYHALYEKLRPHAFAR
                     HPDLAAEPANRALLEGWAKEDARYIIALATETQLGMTVNARNLELMLRRLAAHPLLEA
                     QACGREMYASTKAVAPSLIRYTEPTAYDTRTGTALREACAAWLAERGGPDGPAPADRE
                     AGVPPARLVHVTPEADDRIAAALVYGTSTLPLDACRRAVDAMTPAARADLFQAAFRHL
                     ASHDAVRREFEYADLTYELTMSASCFAQWKRHRMATITVQGYDPALGVTVPPAIREVG
                     MEGAFRDVVARTEETYETIRRAAPEAAPYLLTNAHRRRVLTKINVRELYHIARLRADR
                     HAQWDIRTLTEAMLDLAQKVMPLALMLAAGKDRFPERMSEAFGER"
     gene            13110..>13433
                     /locus_tag="PLW16_08030"
     CDS             13110..>13433
                     /locus_tag="PLW16_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HPB70893.1"
                     /translation="MPIEITPITAKPPREELERRMARVRKAMAAQALDCYICANTENV
                     YYLTNFAYIPFERPFFLVIPAAGRPVMVAPKLEVSHAADRVLVDVAYHTYDEFPAPPG
                     RTFVDA"
BASE COUNT         2349 a         4566 c         3987 g         2531 t
ORIGIN      
        1 cgggcgtctt cgcgcagctt gaagaccctg cgggcgtagt cgtagacgac ccgcccctcc
       61 ggcgtgaggg tgacgcccgc cttttcccgg atcgcgaccg gggccgccag gaccccctcc
      121 atgttctgga tgtgcttggt cagggagggc tgggacaggt tcatccgcct ggcggcgcga
      181 ctgaagttcc cttcctgcac gagaaaaacg agggcctcca gctgctgcag agagatattt
      241 ctgaaggcgt cggatctcat ggcgccggct ctcccgcatt cccgccgtcc cgttgcgaca
      301 ggggcggcgc gcatttcccc gtgaccgacg cgatggcgac ccggagaatg agggtctggt
      361 ggagcgaacg cgccgcatag gtccacggac cggccccgcc gtatttttcc atcaggagat
      421 ccagggattt ttcgacctcc ggagaatcgt cgatcagaca ggcccggccg tagccgatga
      481 cgctgcgata ccgcgtcgtc cagcggcagg cctcgccggc cgcgacgatt tcgtccagga
      541 tgtccatctc gaagcagacc cggtcgttct ttcggaggag gtcgatcttc cggcccttcc
      601 tgcccgtatg gaaatgcagg acgcgatccc ggtaggcgaa atgcatgggg acgatgtagg
      661 acacgccgtc ctcggccagg gccaggcggc agaccggggc cgcggccatg atggcttcga
      721 ggatcttgaa gtccttgatt tccttgtcct gacgggacat cccttccccc ttctttattt
      781 tttgcatgcg taccatgtcc cgtccggtcc tgtcaatcga tccgggtgcg cccccgtccc
      841 atgacccgcc ggcaaacggg ccgtttccgg ggcagggtct cccccggccg ggggaaaggg
      901 cgcctgcgca aaaaaatggg aatcacgccc cgagacaacg gcgcgcgagg acgcgggctt
      961 cccatttaag cggcgttgtg ctatgagaac gctcgatgga tcgaaacccg gacgcaatgg
     1021 agctgtggcg gcaggaacgc ccgctcgtca tcccctgccc gcggggcgcc gcccccggct
     1081 tggcggcgga ggtggcgggc ctgggatttc cggtccgggc cgaaaccgac gccgccgtcg
     1141 agacggccgg aacgttcgac gacacgctga agctcaacct tcatctgcgc acggcgcagc
     1201 gggtgctctt tctcctccag gcctttcggg ccacgacgcc cggggaactc tacgggggac
     1261 tgctcgacct cccctgggag gccatcctcc ccgacgacgg ctacgtgagc gtcacctcgg
     1321 cggtggacca cccgacgatc cgggactcgc agttcgccaa cgtcaagtgc aaggacgcca
     1381 tcgtcgaccg gatccagaag gtccggggcc ggcggcccga ttccggaccc gaacggacgc
     1441 ggtcggtcgt tcatctgtac tggcgcggga aggacgcccg gatctacctg gacacctcgg
     1501 gagagcccct ctcgcggcgc ggctaccgcc tgatcccgct ggacgccccc ctgcaggaga
     1561 ccctggccgc ggcgctcgtc cgggccacgg catggaacgg gacggacccg ttcgtcaatc
     1621 ccatgtgcgg gagcggcacg ctcgccatcg aggcgaccct gatcgcccgg cgaatcgcgc
     1681 ccggcctggg gcggtccaac ttcgggttca tgcacgtccg gggttaccgc gcggcggcct
     1741 ggcaagccct ccgggaggcg gcccggctgg aggtgctcgc caaaccggag ggacggatcg
     1801 tggccacgga catccgcccg gaggccgtgg aggcggcgcg ccggaacgcc cgcgccgccg
     1861 gggtggaccg ctcgatcacg ttcaccgtct gcccgttcga gcacaccccg atcccgccgg
     1921 gccgggggac gctgatcctg aatcccccct acggggagcg gatgggcgag ctggccgccc
     1981 tgggggatac ctaccgggcg atcggcgact ttctcaagca caaggccccg ggctgccagg
     2041 gcttcgtatt cacggggaac ccgggcctcg ccaaacaggt cgggctgcgc acccggcaac
     2101 ggctcgtctt ctacaacggc ggcatcgagt gccggctgtt gcattacgac ctctacgcgg
     2161 gcagccggaa agtgcgcgag gggagcgcgg tccccaatgc ggacggtgaa gaatcccgga
     2221 cccaacggcc gctggaacgc gcccgtcccg ccccggctcc gtcagtcggc cgtggacccg
     2281 ctccaaaaac acgatggatt gaaacgcatg caaaaaaaag aaacccccgg cggtaacgcc
     2341 cgccacccca tcggggtgtt cgattccggc gtgggcggcc tgaccgtcgt ccgggccctg
     2401 atggaacgcc tccccttcga gaacctgatc tacttcgggg atacggcccg ggtgccctac
     2461 ggcgtcaagt cggcggagac gatcaatctg tacgccgccc agatcacgga atttctcttg
     2521 caacgggagg ttaagctgct catcatcgcc tgcaacacga tggcggccgt ggctcacgag
     2581 gccgtggtct cccgctcgcc ggttcccgtc ctcgacgtca tcgacgccgg ggcccggatg
     2641 accatccgga cgacccgcac gaagcacgtc ggcgtcatcg gcacgcccgc gaccgtcaac
     2701 agcaacacgt atgcgcgggc gatccaccgc ctcgatccgg aggtccggat cttctcccag
     2761 gcctgcgccc tgttcgtccc gctggtagag gaaggctggt tcgaccaccc cgtcacgcgc
     2821 ctcacggccc aggagtatct gaagccgatc ctcgcggagg gcatcgacac cctcgtcctg
     2881 ggctgcaccc attatcccct gctcaaggcc ctcctgcggg aggtgacggg gcccgcgatc
     2941 ctcctggtcg attccgcgga ggcgatggcg gacatcacgg ccgatcttct gaccgcccgt
     3001 gaactcgcga atccggaccg ggagccgccc gattaccggt tctacgtgac cgacgtcccc
     3061 ttgcactttc agggcatcgg cgagcggatc ctgggccggt ccctgcccca cgtggagcgg
     3121 gtcagcctgt aacatggacc gtcgcacctt cctttactcc ctcctcgtca ccttcttcat
     3181 caacgccttt ctgaccctgt tttttctctt cccggccgcc ctgcagcggc aggccatgcc
     3241 cctgtcgctc atcggctggg tcatgggcgt cggcagcgtc ggcgccctcc tgagccgtcc
     3301 gatcggcagc accatgaccg aaacgctggg actcatccgg agcctcctgg tcctgtgcct
     3361 gggtctgatc gtggtttcca ttcctctgat ctgggcacga acgttctggc cgctgttctt
     3421 cctgcgggcg ggggggggat ttctcttcgg gatggcggtc gtcaccctga cgacctatca
     3481 ggccttcgcc atccccgtca aacagcgggg cagcaccttc gcctggatcg ggatcgccta
     3541 cgtcgcgccc cagctggtcg tgctgcccct cgcggagtgg ttcgtcacgc gcggccggat
     3601 cgatctctat atgggcctgg cgcccctgtt cgccctgctg accttcctgt tcgccctcgc
     3661 gcttcccccc cctcccgcgg cggcggacaa agaccggtcc cccgccgcgc ctcaggagac
     3721 gccgtgggga agctgggcgg acatcctcgt gattcccggc ttctgggcgc tcgtcctggc
     3781 aaccttcacc ttcgccatgc tcaacggcac caccctgcag tacctgccgg ccttcatcaa
     3841 ggaccagggg ctcatcgcca gttccttcct ggtcgtcaat tccgccaccg ccctgaccgt
     3901 gaggcttttc gcctatcgcc tgatggaccg ccttaaccgc ccccgggcca tcggtctgat
     3961 cctgaccggc atgggaacgg ccctgttcct gctgcactgg gccgacagcc atgccgcctt
     4021 catcgccggc ggcctcctct acgggatgtt catgggcatc ggcttcccca tcatcctcgc
     4081 cctggcgccg gatatctttc ccccgcgcct gatgcccaag ggggtttcca tcagtatgct
     4141 ggccatggac gccggtttcg tcctcgcccc catcttcgcc ggttacctcg gacagcgctt
     4201 cgggctggga ctcgtcttca cggcggtcgg cgccggcgcc gtcctgagcg gcctggcggt
     4261 gaccctggcc aaccggcgga tcatccgggg cgggttctgg caatcgtgat cccggcaata
     4321 gcggcctcaa gattgacttc gcagccaaag cgctaaaggg agaatgcagg ggccgtgaca
     4381 gggctggagc agaggaatgc cctggcgtgg cctcggcctg ccgccatcga ttcctccgcc
     4441 ggatctgagg caacaaccgg gttgacatgt ggatcggttc tccctatata gaaggtcgcc
     4501 tgacgacgag gcggcatcca tcatcaaaaa aaacaggggg actatcagat gcgcattgag
     4561 gaattcaagg aaatcgtcta cgggaaggaa gacagcggca tcgtcaccgt cacgctgaac
     4621 atcccgaaac ggaagaacgc catcagcccg tacaccttgt acgaattgtt ctgggccgtc
     4681 gacgccatgg aaaaggacga gacggcccgg gtgatgatcc tcaccggcgc caaggatccc
     4741 gacaacgacg atcccgccaa agaggccttc tgcagcggcg gctacttcaa cccgaacgcc
     4801 atggccggcg tcagcgacga gatcaaggcc gacatcgaca tgaccgacat cgcccaggcg
     4861 aggctaacgc tcaagatgtg gcagtgcgac aagccgatca tcgccgcggt gaacggcctg
     4921 ttcatcggcg gcggcttcac gatgtgcctg gccggctgcg acctgatcta ctgctccgag
     4981 cacgcatggg cccagctccc cttcatcggc ctcggcatca tccccgagct cgcctccagc
     5041 tacctgctgc cgcgcctgat cggattccag cgggccaagg agatcatgtt cttcggcgat
     5101 cgcatcccgg ccaagaagct cttcgagatg ggaatcgtca acaaggttct gccccacgac
     5161 cagttgttgg cccacgcgcg cgatatggcg ctccggctga tcccgccgaa ggccgccggc
     5221 tacgccgtcc gcaccgccaa gcggaccctc caccagccgc tgttcgacgc gatcgcgacg
     5281 gccctgaacc tggaaaacaa ggggctcaac gactgcttca agacaaccga cttcatggag
     5341 gccctgaccg cccgcgtgga gaaacgcccc ccggccttcc agggaaaata acggcgcggg
     5401 acgacacgct gaggcgtccg cgcgggcgcg tgagacgccg ggagtcgaag acatgcagaa
     5461 ccaacgcgcg ggcaacaccc atgttctccg gctggaccgg ggcgaggaga tcgtcaccga
     5521 actgacccgt ttctgcaagt cgcgggggat tgccctgggc ctcgtcacgg gcatcggggc
     5581 ggtcgaccgg gccacgatcg gcctgttccg ccccgcggag aagcggtacg aggcgacgga
     5641 aatgacgggg gaccacgaga tcctgaacct cgccggcaac gtcacgacga tgaacggcga
     5701 ggtctatctc cacctccacg tcaccctggg cgacgcggcg caccgggcct ggggcgggca
     5761 cctgaacgcc gcgtttgtga gcgccacggc ggagatcttc gtcaccgtcc tcgaaggcgc
     5821 ctgcgaacgg gccatggacg agaccgtcgg gctcaacctg atgcggttcg gatcacccac
     5881 ctcatgacgc ccccgcacgg cacacagagg ccctcttgag gcccggcggc cggatgcaca
     5941 cgccgctgcc gccggagacc ggaaacgacc gggaatccgg aagtcccgcc gtgcgccgag
     6001 cgaccctggt caccgccacc gtgacggcct tcctcacccc gtttatgggg tcggccgtca
     6061 atgtcgcctt gcccagcatc ggccgcgaac tcgccatgga cgccatcgat ctgggctggg
     6121 tggccacggc cttcatcctc gcggcggcgg ccagcctcgt ccctttcggc cgcatcgccg
     6181 acattcacgg acgcaagcgg atcttcatgt ggggcgtcgt cctgttcacc gccgcgtcgg
     6241 gcctctgcgc cctggccgct tcggcccggc ttctcatcct ggcacgggtt atccagggcg
     6301 tgggcggcgc catgatcttc ggaaccggcc ccgccatcct cgtttccgtc ttccccgcgg
     6361 cccagcgggg gcgggtcctc ggcttcaacg tcgccgccgt ctacgtcggc ctgtccatcg
     6421 gcccgttcct aggcggactg ctgacccagc acctgggctg gcggagcatc ttcctcgtca
     6481 tggttcccct gggatgcctg ctcgtttatt acatcctgag acacatgccc gtcgagtggg
     6541 ccgacgcccg gggagagccc ttcgacaccg tcggcgcgct cctctacgcg ttcgggttga
     6601 cggcactcat gtacgggttc tcctgcctcc cggagatgcg cggcgccgga ttcatcgccg
     6661 cgggaggcgc cgggctggtc ctcttcgcgg cctgggaggt gcgcaccgcc tatcccatcc
     6721 tgaacatccg gatcttccgt agcaaccgca ccttcgcgtt ttccaatctc gccgcgctga
     6781 tcaactacag cgccaccttc gccgtctcgt tcctgctcag cctctacctg cagtacataa
     6841 atgggttcac gcccgttcag gccggcgtgg tcctcgtggc caccccggtc atccaggcgc
     6901 tcctttcccc gctggcgggg cgcctctccg accggatcgc cccccgtctc ctcgcctcgg
     6961 gaggcatggc cgccacggtc gtcagtctcg tcatgttcac gcggctcgac gcgaccagca
     7021 cgctgccgta tatcctgacg ggacttgtca tcctgggcgg cggctttgcc ttcttctcct
     7081 cgccgaacat gaacgccatc atgagcgccg ttgaccggag tctgtacggc gtcgcttccg
     7141 gcgtctcggg cgccatgcgc ctgatcggac agatgatgag catgggcatc gcgatgatcg
     7201 ccttcgcgat gacgatgggg tgcacgcgcc tctctccgga ctgctacccc ctgttcctgc
     7261 gcgcgctcac gatcatcttc gccatcctat ccgtcctgtg cacggccggg gtcttcgcct
     7321 ccctcgcccg cgggccgcgc gcccgaacag acgcggccgc ggggccatga tgtggatcgt
     7381 gaacggatag cggccataaa aaaggcggag ccgatcagac tccgcctttc cgacaggccc
     7441 tgaggccttg aacccttcgg ctcagtttct tttccccttg ccgggcccct tggcttttcc
     7501 gccgcgctgg ccacggtcgt cgccatgctt gccgcccttg cgcgcatgcc gcgctttgta
     7561 ttccggcacg tagacgttgt cgtaccactg attgcgcaca aaatagacgg gtcgatcaca
     7621 ggccttgtat ttgtgacaat acttcttcca gtttttctca tgcccgggcg gaacatgcat
     7681 ataaaccggg ggcggcggcg ccacactcac cggcacggga gcgatcacaa cgggcctggg
     7741 gaaaacgagc tccggcttgg gaaagtttcc cacgtcgata tggccgtaaa agcccggctg
     7801 accgatggtg accgaaacgc cgacgtcggc cgcctgggcc gaaacggcaa taaagatcgt
     7861 cacgattacg aagagcatct ttttcatgtt ctttttcctc cctttcatga tcttccgcac
     7921 aggtttctca ggcttcggcg aatggccgtt cagccgccac cgcccgctgg gcaggttccc
     7981 ctgcctgaac cgatcctgcc ggatcgcgcc ccaagaatcc tcccagccgc cgcccgctgt
     8041 caagctcaat tcccgtaaag gtgatcaata tcacttgatc tatgtgatca ccttcacaca
     8101 cctttcgcat ccgagcgggt cgaacggttt tttaaaggat ctgtcgaatc cgccacggac
     8161 catggggata aaacggaagg agggggatag gcaccgaaga cgcgatggaa cacccccgct
     8221 caagacaaaa agccccccct ctcccaacgt tcgggatacg gaacgatgtc agtcattgcc
     8281 gcccagggtg gtcagcagag gaaggctgat ataaaacgtg gcaccccccc agtcgttgtt
     8341 ctccacccga atcactcctc catgatcctt gacaatgccg taggaaatgg aaagcccaag
     8401 ccccggaccc tttttctgcg cccgggtcgt gaagaaggga tcgaagatgt tgttcagatc
     8461 ttcgccctgg atgccgcggc cgttgtcggc aaccacaatc agcagttggt cgcggccgtc
     8521 agccgtcctt tccaggcccg tcgagatttt caatcgttta tccgttttcc cttccagggc
     8581 gagggtggcg ttcgaaatca ggttcatcat gacctgttcg atttttcccc tgtccatggc
     8641 aatcttgggc aaatcgtttc gatagctcac ttcggtgtcg atcatgtcga tggtgaacgg
     8701 cgtgacatat aaatgaagaa caccggcgat gacgtcattg atatcctcga tcgatatctt
     8761 cttgtcggaa acgcgggtca cctgtttcag gctttcggca gtggcggcaa tgcgtttgac
     8821 ctgagatacg caaatgttca attcctcctc cgcgtcggcg gacaggtttt ccatggactg
     8881 catgatctgc aattccatcc cgataatgtt caagggattg aggatttcat gggcaacgcc
     8941 ggcggacagt tgccccacgg cggccagttt ctccgcccgc accagttgat cccaggactc
     9001 ttggaaatcc ctctgcgccc gtttgcgttc tgttatatcc cgcgaaactc cgtgcaggcc
     9061 cgtcaggact ccctgatcgt tgcggatacc actgatgatg ttttcgaccc aacgggttga
     9121 accgtctttg tggtagtgtt ccacttcaag ggtcaccgtt ctgtccaaat ccgccccacc
     9181 ttgcgcttcg agacggaact cctccgccag tttttcagac acggtcctca acgattcggg
     9241 cgttatgtgt tcgctcgcat cctgccgcat tctttcttca ggcgtgaatc cgaacaattt
     9301 ttcgatcgaa ggactgacgt agagcgtccg cagattcaag tccatgatcc acacaatgtc
     9361 ggtcatcttg tccgcaagga gccggtattt tgcctcgctt tcctccaggg cctgtttcgc
     9421 ccggcggcgt tccgtatagt cgatgagggc gccccgatat cccacgacgg tattcaaacg
     9481 ggtcacgggg gcggcggaca tgagaacggg cagggggctg ccgtccttcc ggatcatcgt
     9541 gaattcaaca ttttcgattt tccgcccctc ttggattcct gtgagatgat tctcgacttt
     9601 gtggccatcg tccggcgcaa acaagtcctc cgcattcaga cctttttcga tatcctcctg
     9661 cgtaaatccg aaggtttcga aacaagtgcg attgatcgtg ttaagcatgc ctttcgcatc
     9721 gacctcgaac acggatatgg gcagaaactc caacagctcc cggtattttc tctcgctttc
     9781 ccgaagcgcc cgctcgaccc gttcgcgggc ctcgatttcc ttctcgagct cgacgttgtg
     9841 cttcttgatt ttctgaaact gggtgtcgag attggcacgc gtgagggcgt tttcgagggc
     9901 aaaaccgact tcgcccaaca tgatggaaag catccgctga tcgccgctgt cgaagattcg
     9961 cccctccctc ttgttggaaa ggttgacgac cgcaaccagc ctgttttcga caaaaatggg
    10021 aagactcaaa aacgaagggg tgccgtaagc gggatcgttg gccttgcgcg ttctttcatc
    10081 ctgctcgatg ttttcgacaa gcagcgtctt gccctccgtc acggcgtcct tggcgacata
    10141 atcctcccaa tcgatcaccg cgtccttttc cagcggttcc ttgtggcctt tttcggcaac
    10201 gacgcggagc ttgtacaact tctcgttgta tacctgcgat tcccggccgc tctgcaacag
    10261 agccagtttg cgctgcggga tgctggctcc ctccagacgg agaaccgatc ccgtctgggc
    10321 gccggtgatg aacatcgatt tgtccaacag gagacccaga atccggtcaa aatccttttc
    10381 cgccctcgtg atttctaaaa tgtcctgtat cgtcaaaagc tgggaatgct tctgtctcaa
    10441 ttcgtccgtc gtctgatcca gtttccggac aaggtttttg aatgtggagg aaatgctatg
    10501 aagctcggcg atgtcgtgat gaacgtccgt catcggcccc tcctccgaca tcgctttctc
    10561 cagggattcg gaaatacggg caactttatc gaagatctgc cgaacgacga ggagcccccc
    10621 caacacaagg gcaataacca tgcccagcag gatgagatgg tggtattcga atctgtatcc
    10681 gtcctggtaa aggacaaaag acagcaccag caacgggacg gccaagatga gcgcttcgac
    10741 cagcagaagt ctgtatcgta ttctctccct atccggattc agtggatcca tttcgacgaa
    10801 acctttctat cggcttatgc aaaaaccttt cacgcacgtt ggggcaaagc cgtttgcctt
    10861 gtcgcagccg cgttcctgca ggtcggtctg acgcgagggg atgtacgacc ggacaacctt
    10921 ctggaaggat atcccggcgc cgatgatgga cggcattcgc cgcacgcctg ggactgcttc
    10981 ggctctggat agcggtattg cgcttttcag gggccaccgc ccgctctgca ggttccccct
    11041 gcctgaccac atcccggaag atttcatgat aaggtcctcc cacacaccgc ccgatgtcaa
    11101 gcacaatccc cgtcaaggtg gtgattatca ctcgatatag gtgacaatcg tcacacacgt
    11161 tgagattttc acggctaaac ggctcggacg gtttttcagg ttctgtcgaa tcggccacga
    11221 atcatttgca tgcgaccggc ggattggtat aaggaagggc aggtcgggga gacagcgtaa
    11281 gggggaaccc ggaaccccgg agacggagac ggatcatgaa cggatacgga aaacgatggc
    11341 cgggcctgct gatggcgctt gttgtcttcc tgcccggctt ggcccgcgcg gccgatgacg
    11401 ccctcgacaa cgccgtggcc ctgagcgacg gctacatgtc cctgtaccgg atcgtcgccc
    11461 ccaacgaccg ggtcgtcatc tacgccaacg accgcttcga accctgtccc tgaaccggga
    11521 ggatgcccgc atcatggaga tacggctggc cggctacaac ctggattgtg acattatcga
    11581 tgcgtgcaaa aagtactttc cggatcggga ggattggacg ccggagacga tctccgccgc
    11641 ctacgcgcgg atcagccgca gcccgaagac ggtgaccgaa ctgcgcgccg acgcgcgcgc
    11701 ggaagtggaa aaggcccgcc gttccaacga gaacatcgtc ttcgcgatgg gacacagctc
    11761 cgtcgccgag cacgccgtct tcaacctcga cgtgctgggc gtctcgcggc tcctggtcga
    11821 ggagatcgaa cgcttccgcc tgtgctccta cacggagaaa tcccagcgct acgtcctctt
    11881 ccgggacgac ttcgtcctcc cggaggaggt ccggcgggcg ggcctcgcgg acgccttcac
    11941 ggagacgatc cgggcgcagt atgcctgcta ccacgcgctc tacgagaagc tgcggccgca
    12001 tgccttcgcg cggcacccgg acctcgccgc cgagccggcc aaccgcgccc tgctcgaagg
    12061 ctgggcaaag gaggacgccc gctacatcat cgccctggcc accgaaaccc agctcggcat
    12121 gacggtcaac gcccgcaacc tggagctgat gctccgtcgg ctcgccgccc atcccctgct
    12181 cgaggcgcag gcgtgcggcc gcgagatgta cgcgtcgacc aaggccgtcg ccccatcgct
    12241 catccgctac accgaaccca cggcatacga tacgcggacc gggacggccc tgcgggaggc
    12301 ctgcgcggcc tggcttgccg aacgcggggg accggacggc ccggccccgg ccgatcggga
    12361 ggccggcgtc cctccggcgc gcctggtcca cgtcaccccg gaggccgacg accggatcgc
    12421 cgccgcgctc gtgtacggga cctccaccct ccccctcgac gcctgccggc gcgccgtcga
    12481 cgccatgacg cccgcggcca gggccgatct cttccaggcc gccttccgcc acctggcatc
    12541 ccacgacgcc gtccggcggg aattcgaata cgccgatctg acgtatgaac tgacgatgag
    12601 cgcctcctgc ttcgcgcaat ggaagcgcca ccggatggcc acgatcaccg tccagggcta
    12661 cgacccggcc ctcggcgtca cggtcccgcc ggcgatccgc gaggtcggga tggagggggc
    12721 cttccgcgac gtggtcgcac ggacggaaga gacgtacgaa acgatccgcc gagcggcgcc
    12781 ggaggcggcg ccctatctcc tcacgaacgc ccaccggcgg cgggtcctga cgaagatcaa
    12841 cgtccgcgaa ctctaccaca tcgcgcgcct ccgggccgac cgccacgccc agtgggacat
    12901 ccggacgctg acggaggcca tgctcgatct cgcccaaaag gtgatgcccc tcgccctgat
    12961 gctcgccgcg ggcaaggacc gctttcccga acggatgtcc gaggccttcg gcgagcgctg
    13021 aaggcgcctg tccgacaggc ccgccatgac agaaaagccg gattccggct ttgacgaagt
    13081 cgccaacgtt aacgatgagg aggatgacga tgccgatcga gatcacgccc atcaccgcga
    13141 aacccccgcg cgaggaactg gagcgccgca tggcgcgcgt ccgcaaggcc atggcggcgc
    13201 aggcgctgga ttgttacatc tgcgccaata ccgagaacgt ttactacctc acgaattttg
    13261 cctacatccc attcgaacgc cccttctttc tcgtcatccc ggcggcgggc cggcccgtca
    13321 tggtcgcccc caaactggag gtcagccacg cggcggaccg ggtgctcgtc gatgtcgcct
    13381 atcacaccta cgacgaattc cccgccccgc cgggccgaac cttcgtggac gcc
//
LOCUS       DAOOLJ010000051        13203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5185_length_13203_cov_17.495132, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000051 DAOOLJ010000000
VERSION     DAOOLJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5185_length_13203_cov_17.495132"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1719)
                     /locus_tag="PLW16_08035"
     CDS             complement(55..1719)
                     /locus_tag="PLW16_08035"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPB70894.1"
                     /translation="MIRKISQLGVWVAVLVLIPALVLAAPIGRISHLEGEADLTRVGA
                     KAVPVKVNDPVNAGDILRTKAKTKVEVTFNDGNTLWLAEKTYLKITQYQNREGRRSFF
                     DLFRGKTRAVINTLARRSSLELRTPTAVSGVRGTILIGYFTDGLSGFAFERGQGYGYN
                     RRTPGRVVTIRTGEVMQVSSPTAQPVVRPATANEIEQHLQDTSATEGAGEGPDQEEPQ
                     GEGTQGQDQGDDQGQGQGQDQGQPGQDQGEGQGEDQEGTQDQGTPPGGTDGSGQPTGD
                     GEIPDTSAPPPPGDTPPPPPPPPTPTTFIETPPPELLSYPSVSVATSTVSPTQNTTAT
                     FTLSSDSTMPVTFEYNLNNAGWQVIEGNTLTLAAPSLIAGNNNILFRAKDANGQITEA
                     GQYASKSWAVYNINVITDIVQPTKVNAAAFDLNSNTTAVTFEYQINNSGTWISLSGDT
                     LTLSSLSSGDSNIKFRAKDQYGNVSSVIDYDKTWTVSDLLQQVTLGGLTGTLSGVLPN
                     QELNFTASGTAGAAITTVSDTLSDGSTYDGYLAGIPGSWRGLFTSI"
     gene            complement(1762..3342)
                     /locus_tag="PLW16_08040"
     CDS             complement(1762..3342)
                     /locus_tag="PLW16_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB70895.1"
                     /translation="MQKCLPTAMIQLLVVLLFVFSLPAATFGQTGEPPDPLREGIREY
                     QADNYEEAVEFFKQARQAAPDSTTVAFWLGMAYKQQNNFAEAVGPLSDAVTKTPPLKE
                     ALVELVDILYRLERYDEAAQWIAVAEREKIYPAKTAFLKGMILVKQMKYGEAVEAFEK
                     SKSLDKTYTQSAEFQIGLCYMLDRKYAKASDRFRAAVTQDPLSDLGSFARRYQDLVED
                     RQWVERPLRLTLSALGQYDTNMLTEPKRNDSRYPAIDLRTGKEDSYAMLNTLRMDVVP
                     TLPGGWLFNASYAGTWNLHQNNATSHDIFANTLSVAPGYSFGRVAVNLAGNYTHILKR
                     QPVYREYSQSYSVGPMVRILLTEQQNQILELYGGYTNKDYKNHRRTGTPPLDPNEDQT
                     SRGLDSYVSWMWLFQNGAIFNLKYGFSEDRAEGNNWTNRAHRFTVNTIVPIWKGLKLQ
                     LGGEVTLQDYLHESSFALFDHKKRSDEIYMGTAGLTWDVNKYLSVLAQYNGTRADSNI
                     FVYDYIRNLYSVGLEFRY"
     gene            complement(3750..4394)
                     /locus_tag="PLW16_08045"
     CDS             complement(3750..4394)
                     /locus_tag="PLW16_08045"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB70896.1"
                     /translation="MMMEIWTVDPEAPEPETLARALALLRRGGTVAYPTETFYGLGAD
                     ALNEAAVRKIYAIKGRGFRNPLPVIIGARDDLTSLVADVPDVALPLMRDFWPGPLTLV
                     FRAAPCVPPLLTGGTGKIGIRLSSHPVARELARRLGGPLTATSANLSGRQEAVSARDV
                     RVNLQRPAPNVILDGGPTPGGRGSTVLDVTTDPPTVLREGAVPPDRILRKGPPR"
     gene            complement(4405..4911)
                     /gene="purE"
                     /locus_tag="PLW16_08050"
     CDS             complement(4405..4911)
                     /gene="purE"
                     /locus_tag="PLW16_08050"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPB70897.1"
                     /translation="MKEKKHPAVSVVMGSDSDLPVMEEAITILNAFGVPCEVFLTSAH
                     RSPERTTAFATGAAARGVKVVIVGAGAAAHLAGVIASQTPLPVIGVPIDATALKGLDA
                     LLATVQMPGGIPVATMAIGKPGAKNAALFAVRLLALADKKLHEKLKAYIEQMARDIER
                     KHENLKLG"
     gene            complement(5051..6619)
                     /gene="purH"
                     /locus_tag="PLW16_08055"
     CDS             complement(5051..6619)
                     /gene="purH"
                     /locus_tag="PLW16_08055"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPB70898.1"
                     /translation="MHMNTIKRVLISVTDKTGVVEFARGLADFGVEILSTGGTAAQLR
                     AAGLAVRDVSDYTGFPEMMDGRLKTLHPKIHGGLLALRDNAAHVKAAEGHGIALIDMV
                     VINLYRFEDTVAKAGCTLEEAVENIDIGGPTMLRAAAKNHRFVAVVTDPADYGKILAE
                     MKETGGKVSEATTFALAVKTFQLTARYDAAISNYLGRIDADGGLRPFPDTFTVQFAKA
                     QDLRYGENPHQQAVFYREPATAVAAVANARQLQGKELSYNNIMDADAAWNMVADFERP
                     AAIIMKHANPCGAATAAASLAEAYRKAMETDPVSAFGGIVAFNRPVDQATAEELAKTF
                     LEVILAPAFTDEARAILGAKKNVRVLEIPAAELPGPSGLDFRRVVGGLLVQDRDRAPF
                     DIRQAKVVTKRAPTEDEYQALDFAWRVVKHVKSNAIVYATKDQLVGVGAGQMSRVDSV
                     KIAKMKAILPTEGAVLASDAFFPFRDGVDMAAEAGIKAIIQPGGSVRDEEAIQAADEH
                     GIAMIFTGVRHFKH"
     gene            complement(6837..8165)
                     /gene="purD"
                     /locus_tag="PLW16_08060"
     CDS             complement(6837..8165)
                     /gene="purD"
                     /locus_tag="PLW16_08060"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPB70899.1"
                     /translation="MVNILLIGNGAREHALAEALVRSSEKPRLFACMKANNPGLAALA
                     ERTLIGPYHDLKAIVAFARERRCEFAVVGPEDPLSHGVVDALAAAGIPGVGPTRSLAR
                     LETSKSFTRDLCAKYAIPGNVAYRVFTSLDGVADFLETLPTVVLKPDGLTGGKGVLVQ
                     GDHFETRAEALALVREILAEHPSVTVEERLEGEEFSLQCLCDGIHVVATPPVQDHKRR
                     FADDRGPNTGGMGSYSLADHTLPFLTPADVAAGLAITGRVAEAIHRETGEYYRGVMYG
                     GFMATADGVRLLEYNARFGDPEAMNILPLMETDFVAVCRAVISGTLDALPIRFAKRAT
                     VCKYVVPKGYGLPKDHPDARSTSAKIEVGDVGDARLYHSSVDQRPDGLYMTTSRAIGV
                     VGIADDLAQAEAIAERAVGAIRGPVDHRSDIGTAPLIAKRVRHMQSLRGK"
     assembly_gap    8300..8399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8452..9720
                     /locus_tag="PLW16_08065"
     CDS             8452..9720
                     /locus_tag="PLW16_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB70900.1"
                     /translation="MDPLITLKLVLVGFLFLLSGFFSCSEAALFSLTPLHLHKMAEDR
                     LPFLATVRALTARPKRLLITIVVGNEIVNIAISVFMASIFLSLFPVDGAWMAVIVTTL
                     LLLVFGETLPKTFGVVYTMSLSAFFSPLLNLFSRVEKPVVWALEAISERFVSLLTREG
                     ESPRSALTEREFRTLLDAGEREGAIESAQRDLIHRVFDLGDKPVVEVMVPRVDMFCLP
                     QSLAPRDMLREVVAARHSRVPICGADRDDIVGILLAKDLLAGFRNGEPVTGIQQLLQK
                     PFFIPEVRPAGSVLKDFQARRTQIAIVVDEYGGVSGLVTIEDILEDLFEDLYDEHGIG
                     ETLWRRVDEKTLVVSGKMPFDDLRELLTDLASDEEFETVGGFVFHLFGRLPAAGDAVV
                     HAGRRFRVEKMGRARILRVRIETAETEADG"
     gene            9717..11009
                     /locus_tag="PLW16_08070"
     CDS             9717..11009
                     /locus_tag="PLW16_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB70901.1"
                     /translation="MTLAATVALILFCLALEALFSGGEIALLSSEPHRIRHRAEQGQW
                     SARLAMKLIEKPEWFLATTLTGTNLAVVTSSTLATALFISLYGVARGEAVSGLVMIPT
                     LLIMIVARSVFYQYAERLAVRLAPFIWAASFILLPAVYLISRLSRGTIRLSSGRREAA
                     ASYITRDGLKSLLGEGAAAGDMESRERDMVRRVIDFSDVTVEAVMVPISNTTVLPHTA
                     SVSDALAMAEERRNLRIPVYQDQVFNIVGILNTFDLLGALGAGGQRPERSEDPIAPLV
                     RPVALYVPEKKQVKEVLIELRVRGERMAVVVDEYGGAVGIVTIEDILEEIVGEIEDEY
                     DREEALYRADGPGNWLFSARIGLEKFREVVPVDIPAGDYETLGGFLLFRFGRIPRRRD
                     TLAWGGARFVVEDAGPKVIREVRVLMPVEAGPHGEQAR"
     gene            11014..11817
                     /locus_tag="PLW16_08075"
     CDS             11014..11817
                     /locus_tag="PLW16_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPB70902.1"
                     /translation="MKAKNALSAIVLAAGKGTRMKSDLAKVLHPVCGRPMLHYSLTVA
                     RSVGARKIVCVIGHQADDIRRRFDDPGLIFVEQREQLGTGHAVLQAREAFAGDDGGTV
                     LILCGDVPLLRRETVEALLARHAAAGAAVTVMTTLLADPGHYGRVVTDAAGRVVKIVE
                     ARDATPAEREIREINTGIYCVEAGFLFEAVAGIGNNNAQGEYYLTDIVEMAHRRGLAV
                     RSYVAANSEEVMGINTPAELARACRLMEAGAGATPVARSPEGAGKACTE"
     gene            11814..12806
                     /gene="dusB"
                     /locus_tag="PLW16_08080"
     CDS             11814..12806
                     /gene="dusB"
                     /locus_tag="PLW16_08080"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPB70903.1"
                     /translation="MNIGSLVVDPPVLLAPLAGISNLPFRVLNRRYGCALAFTEMISA
                     TGLVRNMRKTFRYLETAPEDRPLGVQLFGTDPGVLAAAAEIAAARGADLIDLNMGCPV
                     RKVVRTGAGAALMKNPALAFEIMKAVRRVTVLPLTVKLRSGWSDRAVNAPELARLAAE
                     AGLDAVIVHPRTVSQGFGGRADWRIIAEVKRALAIPVVGNGDIRTPADAVRMRAETGC
                     DGVMVGRGALGNPWIFQGIASAGGGREAGRPDPAERNRVIRRHWAAEAAYAGPAVAVR
                     TFRKHLLWYTKGLKGGAVFRQTAGRLTDEAALFAELDRFFSTLAPGDGDQAIKA"
     gene            12920..13144
                     /gene="zapB"
                     /locus_tag="PLW16_08085"
     CDS             12920..13144
                     /gene="zapB"
                     /locus_tag="PLW16_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HPB70904.1"
                     /translation="MEQGKFDELEGKIKTLMARYDGLRKRNQDLEVLLKNKSLELEEA
                     NKQLKGINEERNTVRTKIDSLLDLLQDISE"
BASE COUNT         2114 a         4013 c         4531 g         2445 t
ORIGIN      
        1 taaatgtcgc cattttgtga tatttttcag tcagttatgg tttccggatg gacactagat
       61 ggatgtaaat agccctcgcc aggatccggg gatgccggca aggtaaccat cgtaggtact
      121 gccgtcagac agggtatcgc tgacggtcgt tatagcggcg cccgcggtac ccgatgccgt
      181 aaagttaagc tcctgattcg gaagaacccc tgacagtgtt ccggtcaaac cgcccagcgt
      241 gacctgctgc agaagatcgc ttacagtcca tgttttgtcg taatctataa cgctggagac
      301 atttccgtac tgatccttcg ccctgaactt gatattgctg tcacccgaac tgagtgacga
      361 taacgtcaga gtgtccccgg aaagtgaaat ccatgtcccg gaattgttta tctgatactc
      421 gaatgtaact gccgtcgtat tggaattcag gtcgaaggcg gctgcattta cctttgtcgg
      481 ttgaacaatg tcagtaatga cgttgatatt gtaaacagcc caggattttg atgcatattg
      541 ccctgcttct gtgatctgcc cattcgcatc tttagcccgg aacagaatgt tgttgttgcc
      601 ggcgataaga gatggagcag ccagggtcaa ggtgttgccc tcgataacct gccaccccgc
      661 attgttgaga ttgtattcaa acgtcaccgg catggtggaa tccgaagata acgtaaaggt
      721 ggctgtggta ttttgcgtgg gtgaaaccgt gcttgttgcc acgctgacgg aaggatatga
      781 cagaagctcc ggcggcgggg tttcgatgaa cgtcgtcggg gtcgggggcg gcggaggagg
      841 gggcggcgta tcgcccggag ggggcggcgc ggaggtatcc gggatctcgc cgtcgcccgt
      901 aggctgtccg ctcccgtcgg ttccacccgg gggggtgcct tgatcctgcg tgccttcctg
      961 atcctctccc tggccttcgc cctggtcctg acccggctgg ccctgatcct gtccctgacc
     1021 ttgaccctgg tcgtcaccct ggtcctggcc ctgggtcccc tcgccctgcg gctcttcctg
     1081 atccggtccc tcgcccgcgc cctcggtcgc cgaggtgtcc tgcaggtgct gttcgatttc
     1141 gttggccgtg gcgggacgca ccacgggctg cgccgtggga ctggagacct gcatgacctc
     1201 tccggtccgg atcgtgacga cccggccggg cgtccggcgg ttgtagccgt atccctggcc
     1261 ccgttcgaag gcgaacccgc tcaggccgtc ggtgaaataa ccgatgagga tcgttccgcg
     1321 gacgccggag acggccgtcg gggtgcggag ttcgaggctg gagcggcgcg ccagcgtgtt
     1381 gatgaccgcg cgggtctttc cgcggaagag gtcgaagaag ctccttctcc cctcccggtt
     1441 ctggtactgg gtgatcttga ggtacgtctt ttccgcgagc cagagggtgt tgccgtcgtt
     1501 gaaggtcacc tccaccttcg tctttgcctt cgtgcggagg atgtccccgg cgttgaccgg
     1561 gtcgttgacc ttgacgggga cggccttggc gcccacgcgg gtgaggtccg cctccccctc
     1621 gaggtggctg atccggccga tcggcgcggc gaggacaagg gccgggatca ggacgagaac
     1681 cgccacccac acgcccagtt gcgaaatctt gcgaatcata tgccaccccc tggatgcaga
     1741 aaggatttca atgcgtttcc cctaatatct gaactccaga ccgacggagt acaggttgcg
     1801 aatgtagtcg tagacgaaga tgttcgagtc cgcccgcgtc ccgttatact gggcgaggac
     1861 gctcaggtac ttgttgacgt cccaggtgag cccggcggtg cccatgtaga tctcgtccga
     1921 gcgcttcttg tggtcgaaca gggcgaagga gctctcgtgc aggtaatcct gcagggtcac
     1981 ctcgccgccg agctggagct tcaggccttt ccagatcggg acgatcgtgt tgacggtgaa
     2041 gcggtgcgcc cggttcgtcc agttgttccc ctccgcgcgg tcctcgctga acccgtactt
     2101 gaggttgaag atcgccccgt tctggaagag ccacatccag ctcacgtagc tgtcgagacc
     2161 gcgggaggtc tggtcctcgt tggggtccaa gggcggggtc cccgtccgac ggtggttttt
     2221 gtagtccttg ttggtgtagc cgccgtagag ctcgaggatc tggttctgct gctccgtgag
     2281 caggatccgg accatcgggc ccacgctgta ggactggctg tactcgcggt agacgggctg
     2341 gcgcttgagg atgtgggtgt agttccccgc gaggttgacg gcgacgcgcc cgaagctgta
     2401 gccgggcgcc accgacagcg tgttcgcgaa gatatcgtgg ctggtcgcgt tgttctggtg
     2461 caggttccag gtgccggcgt agctcgcgtt gaagagccat ccgccgggga gggtcggcac
     2521 gacgtccatg cgcagggtat tcagcatggc gtagctgtct tccttgccgg ttctgaggtc
     2581 gatggcggga taccgggagt cgttccgctt cggctccgtc agcatgttcg tgtcgtattg
     2641 tcccagggcg ctcagggtca gccgcagcgg tcgctcgacc cactggcggt cctcgacgag
     2701 gtcctggtag cgtcgggcga acgagccgag atcggacagg gggtcctggg tgactgcggc
     2761 ccggaagcgg tcggaggcct tggcgtattt ccggtcgagc atgtagcaca ggccgatctg
     2821 gaactccgcg gactgggtgt aggtcttgtc gaggctcttg gatttttcga aggcctcgac
     2881 ggcctccccg tatttcatct gcttgacgag gatcatgccc ttgaggaagg cggtcttggc
     2941 ggggtagatc ttttccctct ccgccacggc gatccattgg gcggcctcgt cgtaacgttc
     3001 gagccggtag aggatgtcca ccagctccac gagggcctcc ttcaggggcg gggtcttcgt
     3061 gaccgcgtcg gacagggggc cgacggcctc cgcgaagttg ttctgctgct tgtaggccat
     3121 cccgagccag aaggcgacgg tggtggagtc gggcgccgcc tggcgcgcct gcttgaagaa
     3181 ctccacggcc tcctcgtagt tgtcggcctg gtactcccgg atgccttccc ggaggggatc
     3241 ggggggttcg cccgtctggc cgaaggtcgc ggccggaagc gaaaaaacaa aaaggagcac
     3301 gacgaggagc tggatcatgg cggtcggtag gcacttctgc atcgtgaatt tatcctttct
     3361 ttcaagagag tgctgtgagt tcagcaagag ccaacgtact gggggagaaa atcacacaac
     3421 gtcgacgatg gagaaaagag ccctgaaaaa aatgccaatg aatcacttgg ctcgcatttt
     3481 ataggggtgc gggaggcctt gtcaagcgga tttctcaact tgggaggtag aattgtgaga
     3541 ccaatttcac acagcgagac ttttgcgcaa cagcctattt ttgtcatttt gaccgaaggg
     3601 agaaatcttc aataaatcgc attcatgaaa gatttctcgt cgctcgtgct cctcgaaatg
     3661 acgggatgca aaggcctaat ggcacgtatg aagtggctca cacagcgggc gggtgcggag
     3721 cggcggcgcc ggtggggctg ccgccggggt taccggggcg ggcctttccg gaggatccgg
     3781 tccgggggaa cggcgccctc ccggaggacc gtgggcgggt ccgtcgtgac gtcgaggacg
     3841 gtggagcccc ggccgccggg ggtcggaccg ccgtcgagga tgacgttggg tgcgggtcgc
     3901 tggagattca cccggacgtc tcgcgccgag acggcctcct gccggccgct gaggttggcg
     3961 ctcgtggcgg tcagggggcc gccgaggcga cgggccagtt cgcgggcgac gggatggctg
     4021 gagagccgga tgccgatctt gcccgtgccc cccgtgagga gcggcgggac gcatggcgcg
     4081 gcccggaaga cgagcgtcag cggaccgggc cagaagtccc gcatgagggg aagggcgacg
     4141 tcggggacgt cggcgacgag cgacgtcagg tcgtcacggg cgccgatgat gacggggagg
     4201 ggattccgga acccgcgccc cttgatggcg taaatctttc ggacggccgc ctcgttcagg
     4261 gcatcggccc ccaggccgta gaaggtctcc gtggggtagg cgaccgtccc gcctcgcctc
     4321 agcagggcca gcgcccgcgc cagcgtctcc ggctcgggcg cctccggatc caccgtccag
     4381 atctccatca tcattcagcc cttgtcaccc cagcttcaga ttctcgtgct tgcgctcgat
     4441 gtcccgggcc atctgctcga tgtaggcctt caatttctca tgcaatttct tgtcggcgag
     4501 ggcgaggagg cggacggcga acagggccgc gttcttcgcg ccgggcttgc cgatcgccat
     4561 cgtggcgacg gggatgccgc cgggcatctg gacggtggcg aggagcgcgt ccaggccctt
     4621 gagggcggtc gcgtcgatgg ggacgccgat gacgggcagc ggggtttggg aggcgattac
     4681 cccggccagg tgggcggccg cgcccgcccc gacgatgacg accttgacgc cccgggcggc
     4741 ggccccggtc gcgaaggcgg tggtccgctc cggcgagcgg tgggcggagg tgagaaagac
     4801 ctcgcagggg accccgaagg cattcaggat ggtgatcgcc tcttccatca cgggcaggtc
     4861 cgaatcgctg cccatgacga cgctgacggc gggatgtttt ttctccttca tcgtgtgtcc
     4921 tccgcgttcc gggggtcgtt gcggtttccc ttgcccgtcc ttcccgccta tacgggaatg
     4981 acgaggattt gggcaacctg aaattgcggc atcaaccgtc cttcccgccg gcgcgggaac
     5041 gacggggccc tcagtgcttg aaatggcgga cgccggtgaa gatcatggcg atgccgtgct
     5101 cgtccgccgc ctggatggcc tcctcgtcgc ggacggagcc gcccggctga atgatcgcct
     5161 tgattcccgc ctccgcggcc atgtccacgc cgtcccggaa ggggaagaag gcgtcggagg
     5221 caagaaccgc cccctccgtg gggaggatgg ccttcatctt cgcgatcttg accgaatcga
     5281 cgcggctcat ctggcccgcc cccacgccga cgagctggtc cttcgtcgcg tagacgatgg
     5341 cgttggactt cacgtgcttg acgacgcgcc aggcgaagtc gagggcctgg tattcgtcct
     5401 ccgtgggggc gcgcttcgtg acgaccttgg cctggcggat gtcgaagggc gcgcggtccc
     5461 ggtcctggac gaggagcccc cccacgaccc gccggaagtc gaggccggac ggacccggga
     5521 gctccgccgc cgggatctcc aggacgcgga cgttcttctt cgcgccgagg atggcccggg
     5581 cctcgtcggt gaaggccggg gcgaggatga cctcgaggaa ggtcttggcc agctcctcgg
     5641 cggtcgcctg gtccacgggc cggttgaagg cgacgatgcc gccgaaggcg gagacggggt
     5701 cggtttccat ggccttccgg taggcctcgg cgaggctcgc cgccgcggtc gccgcgccgc
     5761 aggggttggc gtgcttcatg atgatcgccg ccggccgttc gaaatcggcg accatgttcc
     5821 aggccgcgtc ggcgtccatg atgttgttgt aggacagctc cttgccctgg agctggcgcg
     5881 cgttggccac ggcggcgacc gccgtcgcgg gctcgcggta gaagacggcc tgctggtggg
     5941 ggttctcccc gtagcggagg tcctgggcct tggcgaactg gacggtaaag gtgtcgggga
     6001 aggggcggag gccgccgtcg gcgtcgatgc ggccgaggta gttcgagatc gcggcgtcgt
     6061 agcgggccgt gagctggaag gtcttgacgg ccagcgcgaa ggtcgtggcc tcggagacct
     6121 tgccgcccgt ctccttcatc tccgcgagga tttttccgta gtccgccggg tcggtgacga
     6181 cggcgacgaa gcggtggttc ttcgcggccg cccgcagcat cgtcggcccg ccgatgtcga
     6241 tgttctcgac ggcctcttcc agggtgcagc ccgctttggc gacggtgtcc tcgaagcggt
     6301 agaggttgat gacgaccatg tcgatcaggg cgatgccgtg cccctccgcc gccttcacgt
     6361 gggccgcgtt atcgcggagc gccagcaggc cgccgtggat cttcgggtgc agggtcttca
     6421 gccgaccgtc catcatctcc gggaaacccg tgtagtcgga gacgtcccgg acggcgagtc
     6481 ccgccgcccg cagttgcgcc gccgtgccgc ccgtggagag gatctccacg ccgaagtccg
     6541 ccagcccccg ggcgaactcg acgacgcccg tcttgtccgt gacgctgatc aggacccgct
     6601 tgatcgtgtt catgtgcatc gcttttgctc ctctaggatg attttagatg ttttgtacaa
     6661 aagccttccg aggaaggttc cggaacttac tccccgatgg gtcgtcgcca cggggggcgt
     6721 gaggtgctgt ttggtcattt cggccttcgt ccgtgttatt tcgatcttcc tccatgtcat
     6781 ttcgaccgaa gggagaaatc tttaagattt ctcaatcgct tgcgctcctt cgtattttat
     6841 ttcccccgca gggactgcat gtgcctgacg cgcttggcga tcagcggggc cgtgccgatg
     6901 tcggaacggt ggtccaccgg cccccggatc gcccccaccg cccgctcggc gatcgcctcc
     6961 gcctgcgcca ggtcatcggc gattcccacg acgccgatcg cgcgcgaggt cgtcatgtac
     7021 aggccgtccg gccgctggtc caccgacgaa tggtagagcc gtgcgtcgcc cacgtcgccc
     7081 acctcgattt tggccgacgt ggagcgggcg tccgggtggt ccttggggag gccgtagccc
     7141 ttcgggacga cgtacttgca taccgtcgcc cgcttcgcga agcggatggg cagggcgtcg
     7201 agcgtgccgg agatcacggc ccggcagacg gcgacgaagt ccgtctccat caggggcagg
     7261 atgttcatcg cctccgggtc gccgaagcgg gcgttgtatt cgagcagccg cacgccgtcc
     7321 gcggtcgcca tgaacccgcc gtacatgacg ccccggtagt attcgcccgt ctcccggtgg
     7381 atggcctcgg ccacgcgccc ggtgatggcg agccccgccg cgacgtccgc cggcgtgagg
     7441 aagggaaggg tgtggtcggc cagggaatac gatcccatgc cgcccgtgtt cggcccccgg
     7501 tcgtcggcga aacggcgttt gtggtcctgg acgggcggcg tggcgacgac gtggatgccg
     7561 tcgcagaggc actggagtga aaattcctcc ccctccaggc gctcctcgac ggtgacgctc
     7621 gggtgctccg cgaggatctc ccggacgagc gccagggcct ccgccctcgt ctcgaagtgg
     7681 tccccctgga cgaggacgcc cttgccgccc gtcagcccgt cgggcttaag gacgacggtg
     7741 gggagcgtct ccagaaagtc ggccacgccg tcgagggacg taaagacgcg gtaggcgacg
     7801 tttcccggga tcgcgtactt ggcgcagagg tcccgggtga aggacttgga ggtttccagc
     7861 cgggcgagcg agcgggtcgg gccgacgccg gggatgcccg cggccgcgag ggcgtcgacg
     7921 acgccgtggc tcaaaggatc ttccggaccg accacggcga attcgcagcg gcgctcccgg
     7981 gcgaaggcga cgatggcctt gagatcgtga taggggccga tcagggtccg ctcggccagc
     8041 gcggccaggc ccggattgtt ggccttcatg catgcgaaaa gacggggttt ttcagacgac
     8101 cggaccaggg cctccgccag ggcgtgctcc cgggccccgt tgccgataag gaggatgttg
     8161 accatggggg tggattctcc ttgggggtga agcgatttcg gattccttta actgaaaacg
     8221 accggggcgt caacctttac gcgatttccg acaaaacatt tgtcagtaac cggaggaatc
     8281 tgctaaagct taccccatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     8401 ttccgacaaa acatttgtca gtaaccggag gaatctgcta aagcttaccc catggacccc
     8461 ctgattaccc tcaaactcgt cttggtcggc tttctgttcc tcctgtcagg cttcttttcc
     8521 tgctccgagg cggccctgtt ttcgctcacc ccgctgcacc tgcacaagat ggcggaagac
     8581 cgcctgcctt tcctcgccac cgtccgtgcc ctgaccgccc gcccgaagcg cctgctcatc
     8641 acgatcgtcg tgggaaacga gatcgtcaac atcgccatct ccgtgttcat ggcgtcgatc
     8701 ttcctctctc tctttccggt ggacggcgcc tggatggccg tcatcgtcac gaccctcctt
     8761 ctgctggtct tcggcgagac cctcccgaag accttcggcg tcgtctacac catgagcctg
     8821 agcgccttct tctctcccct cctgaatctc ttcagccggg tggagaagcc ggtcgtctgg
     8881 gcgcttgagg cgatctcgga gcggttcgtg tccctcctga cacgcgaggg ggaaagtccg
     8941 cgcagcgcgc tgacggaacg ggagtttcgc acgctcctgg acgcgggcga acgggaaggg
     9001 gcgatcgaga gcgcccagcg ggacctgatc caccgcgtct tcgatctcgg ggacaagccg
     9061 gtcgtggagg tgatggtgcc ccgggtggac atgttttgcc tgccgcaatc gctcgccccc
     9121 cgggacatgc tccgggaagt cgtggcggcc cggcattccc gggttccgat ctgcggcgcc
     9181 gaccgcgacg acatcgtggg aatcctcctt gcgaaggatc tcttggccgg gttcaggaac
     9241 ggagagccgg tcaccgggat ccagcagctc ctgcagaagc ccttcttcat tccggaggtg
     9301 cgacccgcgg gcagcgtcct caaggatttc caggcgcgcc gcacgcagat cgccatcgtg
     9361 gtggacgagt acggcggcgt ctccgggctg gtcacgatcg aggacattct ggaggacctg
     9421 ttcgaggacc tttacgacga gcacgggatc ggggaaacgc tctggcgccg cgttgacgag
     9481 aaaaccctcg tggtttccgg aaaaatgccc ttcgacgacc tcagggaact cctcacggac
     9541 ctcgcctccg acgaggagtt cgagaccgtg ggcggattcg tcttccatct cttcggacgg
     9601 ctccccgccg cgggcgacgc cgtcgtccac gcgggccgcc gtttccgcgt ggagaagatg
     9661 ggccgagccc ggatcctccg cgtgcggatc gaaacggccg agacggaggc ggacggatga
     9721 cgctcgccgc gaccgtggcc ctgatcctct tctgcctggc cctcgaagcc ctcttctccg
     9781 gcggggagat cgccctcctg tcgtccgaac cccaccggat ccgccatcgg gccgaacagg
     9841 gtcagtggtc ggcccgcctg gccatgaagc tcatcgaaaa gccggagtgg ttcctggcca
     9901 cgaccctcac ggggacgaac ctcgcggtcg tgacgagttc gaccctggcg acggccctgt
     9961 tcatctccct ctacggcgtc gcgcgggggg aggccgtctc cggcctcgtc atgatcccga
    10021 cgctcctgat catgatcgtg gcccgaagcg tcttctacca gtacgcggag cggctggcgg
    10081 tgcggctcgc cccgttcatc tgggccgcct cgttcatcct gctgccggcc gtctacctca
    10141 tctcccggct ctcccgggga accatccgcc tgtcttcggg ccgccgcgag gcggcggcgt
    10201 cctacatcac ccgcgacggc ctcaaatccc tgctcggcga gggcgcggcg gcgggcgaca
    10261 tggagagccg cgagcgggac atggtccgcc gggtgatcga tttctccgac gtgaccgttg
    10321 aagcggtcat ggtgccgatc tccaacacga cggtccttcc ccatacggcc tcggtttccg
    10381 acgccctggc catggcggag gagcgcagga atctccgcat ccccgtctac caggatcagg
    10441 tcttcaacat cgtcggcatc ctcaacacct tcgatctgct cggggcgctg ggggcggggg
    10501 ggcagcggcc ggagaggtcg gaggatccca tcgccccgct cgtgcgtccg gtggcgctgt
    10561 acgtgccgga aaagaagcag gtcaaggagg tgctgatcga gctgcgcgtg cggggcgagc
    10621 ggatggcggt ggtcgtcgac gagtacgggg gcgcggtggg aatcgtcacg atcgaggaca
    10681 tcctggagga gatcgtcggc gaaatcgagg acgagtacga cagggaggag gccctctacc
    10741 gggcggacgg gccggggaac tggctcttca gcgcccggat cggcctggaa aagttccggg
    10801 aggtcgtccc cgtggacatc ccggcggggg attacgagac gctggggggg tttctcctgt
    10861 tccgcttcgg acgcatcccc aggaggcggg acacgctcgc ctgggggggc gcccggttcg
    10921 tcgtggagga cgccgggccc aaggtcatcc gcgaggtgcg ggtcctgatg ccggtcgagg
    10981 cggggccgca cggggaacaa gcgaggtaaa tcaatgaagg caaaaaacgc gctgtcggcc
    11041 atcgtgctgg cggcgggcaa gggaacgcgc atgaagtcgg acctggcgaa ggtcctccat
    11101 ccggtctgcg gccgtccgat gctgcactat tcgctgacgg tggcccggtc ggtcggggcg
    11161 cggaagatcg tctgcgtcat cggccaccag gccgacgaca tccgacgccg gttcgacgat
    11221 ccggggctga tcttcgtcga gcagcgcgaa cagctgggga cggggcacgc cgtgctccag
    11281 gcgcgggagg cgttcgcggg cgacgacgga gggacggttc tgatcctctg cggtgacgtg
    11341 ccgctcctgc gccgcgagac cgtggaggcg cttctggcga ggcatgcggc ggccggggcg
    11401 gcggtgaccg tcatgacgac cctcctcgcc gatccgggtc actacggccg ggtggtgacg
    11461 gacgccgcgg gccgggtcgt gaagatcgtg gaggcccggg acgcgacgcc cgcggagcgg
    11521 gagatccggg agatcaacac ggggatctac tgcgtcgagg cggggttcct cttcgaggcc
    11581 gtggccggga tcggcaacaa caacgcccag ggcgaatact acctgacgga catcgtggag
    11641 atggcccacc gccggggcct cgcggtccgg tcctacgtgg cggcgaattc ggaagaggtc
    11701 atgggcatca acacgccggc ggagctggcg cgggcctgcc gtctcatgga ggccggggcc
    11761 ggcgcgacac cggtcgcccg gtcgcctgag ggcgcgggaa aggcctgtac cgaatgaata
    11821 tcggctccct tgtcgtcgat cccccggttc tgctggcgcc cctggccggg atttccaacc
    11881 ttccgttccg ggtcctgaac cgacggtacg ggtgcgcctt ggcgttcacg gagatgatta
    11941 gcgcgacggg gctcgtccgg aatatgcgga agaccttccg ctacctggag acggcaccgg
    12001 aggaccggcc gctgggcgtc cagcttttcg ggaccgaccc cggcgtcctg gccgccgcgg
    12061 cggaaatcgc cgcggcccga ggggccgacc tgatcgatct caacatgggc tgccccgtga
    12121 ggaaggtggt ccggacgggc gccggcgcgg ccctcatgaa gaatccggcg ctggcgttcg
    12181 agatcatgaa ggcggtccgg cgggtgacgg tgctgccgct gacggtgaaa ctccggtcgg
    12241 ggtggagcga ccgcgcggtc aacgcgccgg aacttgcccg gctggcggcc gaggcgggac
    12301 tggacgccgt catcgtccac ccccggacgg tctcgcaggg attcgggggg cgcgcggact
    12361 ggcggatcat cgcggaggtg aagcgggcac tggcgattcc cgtcgtgggg aacggcgata
    12421 tccgcacccc ggccgacgcc gtgcgcatga gggcggaaac gggctgcgac ggcgtcatgg
    12481 tggggcgggg cgccctggga aatccctgga tctttcaggg gattgcgtcc gcggggggcg
    12541 ggcgggaagc cggccggccc gatccggccg agcggaaccg ggtgatccgc cgccactggg
    12601 ccgccgaggc ggcttacgcc ggacctgccg tcgcggtgcg gaccttccgc aagcacctgc
    12661 tgtggtacac gaaaggcctc aaggggggcg ccgtgttccg tcagaccgcc ggccgcctga
    12721 cggacgaagc ggcgctgttc gccgaactgg accgattctt ctcgaccctg gctcccggcg
    12781 acggggatca ggcaattaag gcttgacttt ttaactggga atggcataat ccgaactcag
    12841 aaaaggaaga gaatttctac ctcccgccgg atgggcgggg agggacgtca aaatcgagcg
    12901 ggagggcaac ggacgcagcg tggaacaggg gaaattcgat gaactggaag gcaagatcaa
    12961 gaccctgatg gcccggtacg atgggctgag gaaaagaaac caggacctgg aggtcctgtt
    13021 gaaaaataaa tcattggagt tggaagaggc aaataaacaa ctaaagggga taaacgagga
    13081 aaggaacacg gtacgcacaa agatcgattc actgctggat ttgcttcagg atataagcga
    13141 gtaaggataa ctcttggatg gagcctttga aaaagcgctt cgacatcaag atactggggc
    13201 agg
//
LOCUS       DAOOLJ010000052        12849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5367_length_12849_cov_21.971080, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000052 DAOOLJ010000000
VERSION     DAOOLJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_12849_cov_21.971080"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="acsC"
                     /locus_tag="PLW16_08090"
     CDS             complement(<1..1159)
                     /gene="acsC"
                     /locus_tag="PLW16_08090"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HPB70905.1"
                     /translation="MALTGLQIQKLLPQTNCKECGSNTCLAFAMKLAAKKAELAECPY
                     VSEEARRVLGEASEPPIRTVALGPDRRLLVGGETVLYRHEKTFFHPTLLAVNVVDTDA
                     PEALGAILARIRDYTFTRVGETFGVDLVSVTQRAPEADRFLDLAERAWRETGRPLVLR
                     SPDPAALAAAAARVRGSRSVLCCPGPEAAAPLLAAAREHDHALALAAPDMDALLALAQ
                     TLRDGGFLDFLLTLPAADPAARIQFNTIVRRAALKEAFRPAGHPLLCTLPPGDPLETT
                     AAAATEILKYGGICVLPGFDPAQLSTLFTLRQNIYTDPQKPIQVEPRLYPLGDPTPDA
                     PVFVTTNFSLTYFVVSGEIENSGISAWLAVPECEGMSVLTAWAAGKFNAATI"
     gene            complement(1196..4690)
                     /gene="acsB"
                     /locus_tag="PLW16_08095"
     CDS             complement(1196..4690)
                     /gene="acsB"
                     /locus_tag="PLW16_08095"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF040764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HPB70906.1"
                     /translation="MSKLICASAIDGAKTWIDRAAKKLAETAAVRGDDCRIGFPDTAY
                     ALPIIYALTGERMETVRDLRRILARAAGMLPPRPTDAVWLPYLGSALDAGIAALFACE
                     ILEACKYVSGPDPIEGIWLGAANDIILRERGVEFVDGTAPGFAAITGRAPTNALAVRI
                     ARELQEKNLYVFMAGSTEGKQFAEQLAEEGVQLGWETRLVPFGRDVSALVYALGFANR
                     VALSFGGIKPGDHRANLHYNKDRIFAFVLALGEVDADKYALACGAINYGFPIIADTAI
                     PQILPTGVCTYEHVVSSVPYDTMVDRALEVRGCKIKIDRVPIPVPYGPAFEGERVRKE
                     DAQAEFGGNLTPAFEFVTSVGMDEIEDGVVEIIGPDIDAVPPGTALPLAIWAEVAGRK
                     MQPDFESILERQIHHLVNGAEGIWHMGQRDIVWTRVSKAGFARGLRLRHYGDILHARF
                     LSQFPAIVDKVKVTIVTDTAEVERRLAVARRVYDERNRRLASLKDEAVDVFYSCLLCQ
                     SFAPHHVCVITPERLGLCGAYNWLDGKAAYEIDKTGPNQPIQKGECFDPVRGIWRGVN
                     DYVYVNSHKTVDTFCLYSIMDRPMTSCGCFEVICAYVPECNGIMAVNREFTGDTPVGM
                     TFSTLAGNVGGGAQTPGFMGIGKVYLTSRKFLFAEGGHARLVWMPKDLKNLLAADLQP
                     VLAEQGRADLLEKIADETVATDPAEIRRFMERVGHPALALPDLSAAIAGTAGAPAAVP
                     ASDPVPGTPAVESSASGIPGGAEPAPKAALPSDLLTGIGEELKKTLAREIVAEVMAAL
                     ERRLGVPPAESTGPADPDAASPSSDREPSAASAPAAGEAIAAIRAFPVRRETATVPVH
                     TVALGATKAEGGTRGRVLRIGGATAMPFHRWEGEMPHRPLVAMEVFDRVSEKYPDILR
                     AEWGDLLDRPADMARACVERCGADLISVRLDGTHPEKGGRSPLEALALVREVLAAVDC
                     PVIVTGHNHFETWNEVAREVARGCAGENLLLNWAEQENFRTIAGMALAYGHTVVAQSP
                     IDVNIAKQTNILMTNMDLPLDRIVIDPLTGALGYGIEYCYSVMERIRQTGLGGDPLLA
                     GPMIVCPGAECAKVKEYRAAEADFPAWGDRERRAALWELTTALALLYAGADLLVMYHP
                     EAVRVLKKQILRLLDGRP"
     gene            complement(4683..6635)
                     /gene="cooS"
                     /locus_tag="PLW16_08100"
     CDS             complement(4683..6635)
                     /gene="cooS"
                     /locus_tag="PLW16_08100"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HPB70907.1"
                     /translation="MAEKPLRPPEQCACDAASVAALHKAGREGLTLSFQRLDRMGAPC
                     GFGVSGVCCRICHMGPCRITPKHPAGICGADADTIVARNFLREVAGGAAAHSDHARHL
                     VLLLKDVAEGKSPDYALKDPSALRRAGARYGLAPEGRTDPELALALAGTLLNEFAAQE
                     ETLATLRLAPLKRQSLWTARELAPAGIDRMIVEAMHRTHIGVDHDYAHLLLHAFRTAL
                     ADGWGGSRIASVVSDILFGTPSPVRSRVNLGVLEEATVNVVVHGHEPALSEMLAAASL
                     DPEILSQARQAGAAGITLAGLCCTANEILMRHGIPIAGSFLQQELAIVTGAVEMMIID
                     VQCCMPGLPDVARAYHTEVVSTSAIARTIGATNMPFHAEDAYTQAKALLARAIVNFAR
                     RDPAKVAIPAVTEPLVAGFSVEAIKYMLGGSYRASFRPLNDAVIQGRIAGVAGIVGCN
                     TPRERMDDYTNALTRELLAQNVLVLKTGCAAIASAKAGMLTPEAAFAAAGPGLREVCE
                     AVGMPPVLHMGSCVDNSRLLEAAAEIVAEGGLGDDIAQVPVVGVAPEWMSEKAISIGC
                     YFVASGIDVILGHPFHISGSERVTRFLNEDVREIGGASFHVCPTPGEAAATVMRLLTE
                     RRRRLGIDRQAERKLMDMADRRKLDV"
     gene            complement(6644..7405)
                     /locus_tag="PLW16_08105"
     CDS             complement(6644..7405)
                     /locus_tag="PLW16_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB70908.1"
                     /translation="MSRTIAITGKGGTGKTTLAGLIVGGLIRAGATPVLAVDADPNMC
                     LDEVLGVRATQSVGHVREKIRDEDAQGIAKRTLLERKIAESLVEAAGFDLIAMGRPEG
                     PGCYCYANNILREVLALMTRQYPFTVIDNEAGLENLSRRIAPAVDLLVLVGDPSRRGI
                     ATIRRLHDLAREMDVRFGKLAVVVNRVRPSSAFRPDLSDLPMDALFTLPEDDDLAHWG
                     EEGRSLLEFPGNHPLAQRIDAFLKEQLGVPIRGAA"
     gene            complement(7402..8502)
                     /locus_tag="PLW16_08110"
     CDS             complement(7402..8502)
                     /locus_tag="PLW16_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70909.1"
                     /translation="MNLPRFTATAVGSFPHTDPDDALDIIFRALPTAPLWPQLPRAAL
                     TEQMEIQYAEGIPGTVLDEARQRLTFDTAGDPSEALAAFYEVYLAAVEAGPGEADLAP
                     LAIGPSFARGLHRFAERLTAEGIRRSLVKVQTTGPLSFALTVVDQDKRAIYYDETFRD
                     VIVKALALKCRWQIRTFAPWAERVLCFIDEPMLAAFGSSTYISVTRGDVTSLIGEVVD
                     AIHAEGGLAGVHCCGNTDWSLLVKAGVDVINFDAFGYGESVALYPEALRTFLDRGGLL
                     AWGIVPTSERVREQTAASLADRFDKLVRTIAGLAGLDRARLVETALLTPSCGTGSLSV
                     DDAGRVFALLAELPAVLTNRYGAGDIRIQERA"
     gene            complement(8499..9284)
                     /locus_tag="PLW16_08115"
     CDS             complement(8499..9284)
                     /locus_tag="PLW16_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB70910.1"
                     /translation="MKWAVSGKGGVGKSTLTAALALLLAETGRKVLAVDADPDANLAS
                     FLGIPLAEQTAILTIADQKTLIEERTGVKLKDYGGMFKLNPTVDDLAERFAHAHRGVR
                     LLVLGAVRRGGTGCACPENTLLRALVQDLVLNREETLLLDMEAGIEHLGRATVRGVDG
                     LLVVLAPGQRAVEAFHRIRRLAADIGLSRIRAVLNGVRTAEEEAFLRQALPGIDIAGV
                     IPEAAGLRAADRDGMSVLEGLDAATRVALTALRDHLFSQEAST"
     gene            9514..11604
                     /locus_tag="PLW16_08120"
     CDS             9514..11604
                     /locus_tag="PLW16_08120"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPB70911.1"
                     /translation="MHGKIRKRDGRLVKFKADKITNAIARAGAATGEFDAQTAQKLTI
                     RVLNLAEKLYDGNIMTVENAQDIVEEVLLDSPYRKTAKAYIIYRDQHARLREITQKME
                     VDLVDQYLKKKDWKIHENSNMDYSLQGLNNYISSEVSKVYWLNEIYSPEIRKAHTDGD
                     FHIHDLSLLSVYCVGWDLYDLLFEGFRGVSGKVESRPANHLRSALGQIVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRYDGLNYSEIRQALQEFIFNINVPTRVGFQTPFTNVTLDV
                     TVPVYYADQPVLVGGKLQKETYGAFQEEMNLFNRAFVEVMAEGDAKGRVFTFPIPTYN
                     VTREFDWEDPNLAGLWEMTAKYGVPYFSNFINSDMKPEDARSMCCRLRIDNRELEKRG
                     GGLFGSNPLTGSIGVITMNMPRIGHLSRSEDEYLQRLERLMGIARESLETKRKVLEAF
                     TDGNLYPYTKYYLRKVKERFGEYWKNHFSTIGLVGMNEACLNLLGKDLASPEGQRFTK
                     RVLDFMRERLVHFQRETGNNYNLESTPAEGTSYRLARIDKEKYPEILCANESRLREAQ
                     AEPFYTNSSQLPVNYTDDIFEALDLQDEIQSKYTGGTVFHTFAGERVDDPEAVKALVR
                     KVCTRYHLPYLTFTPTFSVCPSHGYLKGEQEACPACRMPCEIYSRVVGYLRPIRQWNK
                     GKQSEFQLRREYQF"
     gene            11633..12319
                     /locus_tag="PLW16_08125"
     CDS             11633..12319
                     /locus_tag="PLW16_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPB70912.1"
                     /translation="MNIGGFQKVSLIDYPGRISAVIFTQGCNFRCPFCHNPELVDSAR
                     FGPTLSEAEIFAFLEKRRGTLDGVSITGGEPTLQPDLAACATRLKGMGFLVKIDTNGS
                     RPEVLRDLIDRRLIDAVAMDVKGSLKKYDRLTGVRNSGRTVEESIRVILASGLTHEFR
                     TTVVPSLLTERDFLEIGRLVEGAASFVVQTYVRTKQLDPAVPPPGAEPPDLNKCAARV
                     APHVSRVRIR"
     gene            12447..>12849
                     /locus_tag="PLW16_08130"
     CDS             12447..>12849
                     /locus_tag="PLW16_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HPB70913.1"
                     /translation="MRTSTTHRQNADRAMVKAGPANTQAVLEAAAWRAEACKIRKILI
                     ATCSGRTALAAVQAFPPETEIIAVTHVTGFAKPNHQELTPANRRRLEEKGVAIFTGQH
                     AFGGVGRGIRNRLGAYQVDEIMAFTLRTFGQG"
BASE COUNT         2092 a         4226 c         4490 g         2041 t
ORIGIN      
        1 cgatggtcgc ggcgttgaat tttcccgcgg cccaggcggt caggacgctc atcccctcgc
       61 attcgggtac ggcgagccag gcgctgatgc cgctgttttc gatctccccg gagacgacga
      121 aataggtgag ggagaaattc gtcgtgacaa agacgggcgc gtccggcgtg gggtccccaa
      181 gggggtagag cctgggttcc acctggatcg gcttctgcgg atccgtgtag atgttctggc
      241 gcagggtgaa gagcgtcgag agctgggccg ggtcgaaccc cggcaggacg cagatcccgc
      301 cgtacttcag gatctccgtc gcggccgccg cagtggtttc gagcgggtcc ccgggcggaa
      361 gtgtgcacag gagcgggtgg cccgccgggc ggaacgcctc cttcagcgcc gcccgtcgga
      421 cgatcgtgtt gaactggatc cgggcggccg ggtcggcggc ggggagcgtg agcaggaagt
      481 cgagaaaccc cccgtcccgc agcgtctgcg ccagggcgag cagggcgtcc atgtccgggg
      541 cggcgagggc cagggcgtga tcgtgttccc gggccgcggc gagcaggggc gccgccgctt
      601 cggggccggg gcagcacagg acgctgcggg aaccccgcac gcgggccgcg gccgccgcga
      661 gggcagcggg gtcgggactc cggaggacca ggggccggcc ggtctcccgc caggcgcgtt
      721 cggccaggtc gaggaaccgg tccgcctccg gggcgcgctg ggtgacggag acgagatcga
      781 cgccgaaggt ttcgcctacg cgggtgaagg tgtagtcccg gatgcgggcc aggatcgcgc
      841 cgagggcctc cggggcgtcc gtgtcgacga cgttcaccgc caggagcgtg gggtggaaga
      901 aggtcttctc gtgccggtac aggacggttt cgccgccgac gagcagccgg cggtccggcc
      961 ccagggcgac ggtcctaatc ggcggctcgc tcgcctcgcc gaggacccgc cgcgcctcct
     1021 cggagacgta ggggcactcc gccagctccg ccttcttggc ggcgagcttc atggcgaagg
     1081 cgaggcaggt gttcgacccg cattccttgc agttcgtctg cgggaggagc ttctggatct
     1141 gcaagcccgt taaggccatg tcacctccag gggaaaatgc cgctccgggt gggcgtcacg
     1201 ggcgcccgtc caggagtcgg aggatctgct ttttcaggac gcggacggcc tcggggtggt
     1261 acatcacgag gaggtccgca ccggcgtaca ggagcgcgag cgccgtcgtc agctcccaga
     1321 gggctgcgcg ccgctcccgg tcgccccaag cggggaagtc ggcctccgcg gcccggtatt
     1381 ccttgacctt cgcgcactcc gcccccgggc agacgatcat cggcccggcc aggagcgggt
     1441 cgcccccgag gcccgtctgg cggatccgct ccatgacgga gtagcagtac tcgatcccgt
     1501 agccgagcgc tccggtcagg ggatcgatga cgatccggtc gaggggcagg tccatgttgg
     1561 tcatcaggat gttcgtctgc ttggcgatgt tcacgtcgat cgggctctgg gcgacgacgg
     1621 tgtggccgta ggcgagcgcc ataccggcga tggtcctgaa gttttcctgc tccgcccagt
     1681 tcagcagcag gttctccccg gcgcagccgc gggcaacctc ccgagcgacc tcgttccacg
     1741 tttcgaaatg gttgtggccc gtcacgatta cggggcagtc tacggcggcg aggacctccc
     1801 ggacgagtgc cagcgcctcc aggggcgagc gcccgccctt ctccgggtgg gtcccgtcga
     1861 ggcggacgct gatcaggtcg gcgccgcagc gctccacgca ggcgcgggcc atgtccgcgg
     1921 gccggtcgag taggtcgccc cactcggccc tcaggatgtc ggggtatttt tccgacaccc
     1981 ggtcgaagac ctccatcgcg acgagcggcc ggtggggcat ctcgccctcc cagcggtgga
     2041 agggcatggc cgtcgccccg ccgatgcgga gcacgcggcc gcgcgttccg ccctccgctt
     2101 tcgtagcccc gagcgcgacc gtgtggacgg ggaccgtcgc cgtctcccgc cgcacgggga
     2161 aggcgcggat ggccgcgatc gcctctcctg cggccggcgc cgaagcggcc gaaggctccc
     2221 gatcggatga cggggatgca gcgtccgggt cggccgggcc ggtagactcc gcgggcggaa
     2281 cccccagacg ccgttcgagg gcggccatca cctcggccac gatctcccgg gcgagggtct
     2341 ttttcaactc ctccccgatt ccggtgagga ggtcggacgg cagcgccgcc ttcggtgcgg
     2401 gctccgcgcc gccggggatt cccgacgcgg acgattcaac cgccggtgtc ccgggaacag
     2461 gatcggaggc cggtacggct gccggtgcac ccgccgttcc ggcaatggcg gcggacagat
     2521 cgggaagggc cagcgcgggg tgccccacgc gctccatgaa gcggcggatc tcggccggat
     2581 cggtggcgac ggtctcgtcg gcgatcttct cgaggaggtc cgcccggccc tgctcggcca
     2641 ggacgggttg gaggtccgcc gccaggaggt tcttcaggtc cttcggcatc cagacgaggc
     2701 gcgcatggcc cccctcggcg aagaggaact tccgggaggt caggtacacc ttgccgatcc
     2761 ccatgaatcc aggcgtctgc gcgcctccgc cgacgttccc ggcgagcgtc gagaaggtca
     2821 tgccgacggg ggtgtcgccg gtgaactcgc ggttgaccgc catgatgccg ttgcattccg
     2881 ggacgtaggc gcagatcacc tcgaagcagc cgcagctcgt catcggccgg tccatgatcg
     2941 aatacaggca gaaggtgtcg accgtcttgt gggagttgac gtacacgtag tcgttgacgc
     3001 cccgccagat cccccgcacc ggatcgaagc attccccctt ctggatcggc tggttggggc
     3061 ccgtcttgtc gatctcgtag gcggcctttc cgtcgagcca gttgtaggcc ccgcacaggc
     3121 cgaggcgctc cggcgtgatc acgcagacgt ggtggggagc gaaggactgg cacaggaggc
     3181 acgagtagaa cacgtccacc gcctcgtcct tcaggctggc cagacgccgg ttgcgctcgt
     3241 cgtagacccg gcgggcgacg gcgaggcgcc gctccacctc ggcggtgtcg gtgacgatcg
     3301 tgaccttcac cttgtccacg atggccggga actgggagag gaagcgggcg tggaggatgt
     3361 cgccgtagtg gcgcaggcgc agcccccggg cgaagcccgc cttggagacg cgggtccaga
     3421 cgatgtcgcg ctgccccatg tgccagatcc cctccgcgcc gttgacgagg tggtggatct
     3481 gccgctccag gatcgactcg aaatcaggtt gcatctttcg tccggcgacc tccgcccaga
     3541 tcgcgagcgg cagcgccgtg cccgggggga ccgcgtcgat gtccgggccg atgatctcga
     3601 ccacgccgtc ctcgatctcg tccatgccga cggaggtgac gaactcgaag gccggggtca
     3661 ggttgcctcc gaactccgcc tgggcgtcct ccttgcggac ccgctccccc tcgaaggctg
     3721 gaccgtaggg gacggggatc ggcacgcggt cgatcttgat cttgcagccc cggacctcca
     3781 gggcgcggtc caccatcgtg tcgtacggaa cggaggagac gacgtgctcg taggtgcaga
     3841 cgcccgtggg caggatctgg gggatcgccg tgtcggcgat gatggggaag ccgtagttga
     3901 tggccccgca ggcgagcgcg tacttgtcgg cgtccacctc gcccagggcg agcacgaagg
     3961 cgaagatgcg gtccttgttg tagtgcaggt tggcccggtg gtctccgggc ttgattcccc
     4021 cgaaggacag ggcgacgcgg ttggcgaagc cgagggcgta caccagggcg gagacgtcgc
     4081 gcccgaaggg aacgaggcgc gtctcccacc cgagctggac cccctcttcg gcgagctgtt
     4141 cggcgaactg ctttccttcc gtggacccgg ccatgaagac gtagaggttc ttttcctgga
     4201 gttcgcgggc gatccggacg gcaagggcgt tcgtcggggc ccgtccggtg atggcggcga
     4261 agccgggggc ggtgccgtca acgaactcga ccccacgctc gcgcaggatg atgtcgttcg
     4321 ctgcgcccag ccagatgcct tcgattgggt ccgggccgct gacgtacttg caggcctcga
     4381 ggatttcgca ggcgaagagc gcggcgattc ctgcgtcgag ggcgctgccg agatagggga
     4441 gccagaccgc gtcagtggga cggggtggga gcatgccggc ggcgcgggcg aggatgcgcc
     4501 gaagatccct gacggtctcc atccgctcgc cggtcagcgc gtagatgatg ggcagcgcgt
     4561 aggcggtatc ggggaagccg atccggcagt cgtccccccg gacggccgcg gtctccgcca
     4621 gcttcttcgc cgcccggtcg atccaggtct tggccccgtc gatggcgctc gcgcagatca
     4681 gcttagacat cgagcttcct ccggtcggcc atgtccatca gcttccgctc ggcctggcgg
     4741 tcgattccga ggcgccgccg gcgttcggtg agcagccgca tcacggtcgc cgccgcctct
     4801 cccggggtcg ggcagacgtg gaaggaggcc ccgccgatct cccggacgtc ctcattcaaa
     4861 aatcgagtca cgcgctccga gccggagatg tggaaggggt gtcccaggat cacgtcgatg
     4921 cccgaggcga cgaagtaaca gccgatggag atcgcctttt cgctcatcca ctccggcgcg
     4981 acccccacga cggggacctg ggcgatgtcg tcgccgaggc cgccctcggc cacgatctcg
     5041 gcggctgcct cgagcaggcg cgaattgtcc acgcaactgc ccatgtgcag gactggcggc
     5101 atgccgacgg cctcgcagac ctcgcggagg cccggcccgg cggcggcgaa ggccgcttct
     5161 ggcgtcagca tccccgcctt ggccgaggcg atcgccgcac agcccgtctt caggacgagg
     5221 acattttggg cgaggagctc gcgggtcagg gcgttcgtgt agtcgtccat ccgttcccgg
     5281 ggggtgttgc agccaacgat cccggcgacg ccggcgatcc gcccctggat gacggcgtcg
     5341 ttgagcggac ggaaggaagc ccggtacgac ccgcccagca tgtatttgat ggcctcgacg
     5401 ctgaagccgg cgacgagcgg ttcggtcacg gcggggatgg ccaccttggc cggatcgcgc
     5461 cgcgcgaaat tcacgatcgc gcgcgcgagc agggccttgg cctgggtgta ggcgtcctcc
     5521 gcatggaagg gcatgttcgt cgccccgatc gtccgggcga tggcgctcgt ggagacgacc
     5581 tccgtgtggt aggcccgggc gacgtccgga agccccggca tgcagcactg gacatcgatg
     5641 atcatcatct ccaccgcccc cgtcacgatg gccaactcct gctggagaaa actgccggcg
     5701 atcgggattc cgtggcgcat gaggatttcg ttggcggtgc agcagagccc cgccagggtg
     5761 atccccgccg cgccggcctg ccgtgcctgg gagaggattt cggggtccag ggaggcagcg
     5821 gcgagcatct ccgagagggc gggttcgtgg ccgtggacga cgacgttgac cgtcgcctcc
     5881 tccagaacac ccagattcac gcggctgcgg accggcgacg gcgttccgaa gaggatgtcc
     5941 gacacgacgc tcgcgatgcg cgatccgccc cagccgtcgg cgagggccgt gcggaaggcg
     6001 tgaagaagca ggtgggcata gtcgtggtcc acgccgatgt gggtccggtg catggcctcg
     6061 acgatcatcc ggtcgatgcc ggccggcgcg agctcccggg cagtccagag gctttgccgc
     6121 ttgagcggcg cgagccgcag ggtcgcgagg gtctcctcct gcgcggcgaa ttcgttgagc
     6181 agcgtcccgg cgagcgccag ggccagttcg ggatcggtcc gtccctccgg cgcaaggccg
     6241 tagcgcgcgc ccgcccgccg gagggccgac gggtccttga gcgcgtagtc cgggctcttt
     6301 ccttcggcta cgtccttcag gagcaggacg aggtgacggg catggtcgga atgtgccgcc
     6361 gccccgcccg ccacctcccg gaggaagttg cgggcgacga tcgtgtcggc gtccgccccg
     6421 cagattccgg cggggtgctt gggcgtgatc cggcacggcc ccatgtggca gatccggcag
     6481 cagacgccgc ttacgccgaa accgcacggg gcgcccatcc ggtccaggcg ctggaagctc
     6541 agggtcagcc cctcccggcc ggccttgtgc agggcggcga cggaggcggc gtcgcaggcg
     6601 cattgttcgg gggggcggag cggtttctcg gccatggggt ccctcacgcg gcgccgcgga
     6661 tcgggacgcc cagctgttcc ttcagaaagg cgtcgatccg ttgcgcgagc gggtggtttc
     6721 cgggaaattc gagcagggat cttccctcct cgccccagtg ggccaggtcg tcgtcttcgg
     6781 gcagggtgaa gagcgcgtcc atgggcaggt ccgacaggtc gggccggaac gccgaagacg
     6841 gccggacccg gttcacgacg acggcgagtt tcccgaagcg gacgtccatc tcgcgggcca
     6901 gatcgtggag ccgccggatc gtggcgatcc cgcgccggga agggtcgccg accagcacga
     6961 gcagatccac cgccggggca atgcggcggg agaggttctc caggccggcc tcgttgtcga
     7021 tcacggtgaa gggatactgg cgggtcatga gcgccaggac ctcgcgcagg atgttgtttg
     7081 catagcagta gcagcccggc ccctccggac gccccattgc gatcaggtcg aaaccggcgg
     7141 cctccacgag gctctcggcg atcttccgct ccagaagcgt ccgcttggcg atgccttggg
     7201 cgtcttcgtc ccggatcttc tcccgcacgt gccccaccga ctgggttgcc cggacgccca
     7261 agacctcgtc caggcacatg ttggggtcgg catcgacggc gagcaccggg gtcgccccgg
     7321 cgcggatcag acccccgacg atcaggccgg ccagggtggt cttgcccgtg ccgcccttgc
     7381 cggtgatggc gatggtccgg ctcatgcgcg ctcctgaatg cggatatcgc ccgcgccgta
     7441 gcggttcgtc agaacggccg ggagttccgc cagcagggcg aagacccgtc cggcgtcgtc
     7501 gaccgacagc gaccccgtgc cgcaggaggg ggtcagcagg gcggtttcga ccaggcgcgc
     7561 ccggtccagt ccggcgagcc cggcgatcgt gcggaccagc ttgtcgaacc ggtccgccag
     7621 ggacgccgcc gtctgctccc ggacccgctc cgacgtcggc acgatccccc aggcgagcag
     7681 gccgccgcgg tcgaggaagg tccgcagggc ctccgggtac agggcgacgc tctcgccgta
     7741 gccgaaggcg tcgaaattga tgacgtccac cccggccttc accaggaggc tccagtccgt
     7801 gtttccgcag cagtggacgc ctgcgagccc cccctccgcg tgaatggcgt cgaccacctc
     7861 cccgatgagc gatgtcacgt cgccgcgggt gacggagatg tacgtcgacg acccgaaggc
     7921 ggcgagcatg ggctcgtcga tgaagcacag gacgcgctcc gcccaggggg cgaaggtccg
     7981 gatctgccac cggcatttca gggccagggc cttgacgatc acgtcccgga aggtctcgtc
     8041 gtagtaaatg gcccgcttgt cctggtccac gaccgtgagg gcgaaggaca gcggtcccgt
     8101 cgtctggacc ttcacgagcg aacggcggat gccctccgcc gtcaggcgtt cggcgaagcg
     8161 gtggagcccc cgggcgaaag acggcccgat ggcgaggggc gcgaggtcgg cctctcccgg
     8221 tccggcctcg acggcggcga gatagacctc gtagaatgcg gccagggcct cggagggatc
     8281 gcccgcggta tcgaaggtca ggcgctgcct tgcttcgtcc aagacggtgc cggggatgcc
     8341 ctcggcgtac tggatctcca tctgctccgt gagggccgcc cggggcaact gtggccacag
     8401 cggggccgtt ggcagggcgc ggaagatgat gtccagggcg tcgtcgggat cggtatgggg
     8461 aaaagagccg acggccgtgg ccgtgaagcg cggcaggttc atgtggacgc ctcctgggaa
     8521 aaaagatgat cgcgcagggc cgtcagggcg acgcgggtgg ccgcgtccag gccctccagg
     8581 acggacatcc cgtcacgatc ggccgcgcgc aggcccgccg cttccgggat cacccccgcg
     8641 atgtcgatgc cggggagcgc ctgccggagg aaggcctcct cctccgcggt gcggacgccg
     8701 ttcaggacgg cgcgaatccg cgagaggccg atgtccgcgg ccaggcggcg gatccggtga
     8761 aacgcctcga ccgcccgctg tccgggcgcg aggacgacca gcagtccgtc caccccccgc
     8821 accgtggcgc gtcccagatg ttcgatgccc gcctccatat ccagcagcag ggtttcctcc
     8881 cggttcagga cgaggtcctg cacgagggcg cgcagcagcg tgttctccgg gcaggcgcag
     8941 cccgtcccgc ccctccggac ggccccgagc acaagcagcc ggaccccccg gtgcgcatgg
     9001 gcgaaccgtt ccgccaagtc gtcgaccgtc gggttcagct tgaacatccc gccatagtcc
     9061 ttgagcttga cgccggtgcg ctcctcgatc agggtcttct ggtcggcgat ggtcaggatg
     9121 gcggtctgct cggccagcgg aatgcccagg aaggaggcca ggttcgcgtc ggggtcggcg
     9181 tccaccgcca gcaccttccg ccccgtctcg gccagcagca gcgccagcgc ggcggtcagg
     9241 gtagacttgc cgacgccccc cttgcccgat acggcccatt tcatggatgt tccaccgttc
     9301 ttcccccgcg cgttttcggg gggcaccata agattgacgg caaaaaaagt caacgacttt
     9361 tccctgcgac gcggggaggc gttttgggag ggggagcgca tttttccgct tgcctgtatc
     9421 aatatatggg ggtataagaa aaactcaacc ccaatatatt gatacaagct ctgccgcgca
     9481 cgaggcgccg gccgggaaag ggagggacct ttcatgcacg ggaagatcag aaagcgggac
     9541 gggcggctcg tcaagtttaa agcagacaag atcacgaacg ccatcgcgcg ggccggcgcg
     9601 gcgacgggtg agttcgacgc ccagacggcc caaaaactca cgatccgggt cctcaacctg
     9661 gcggagaagc tctacgacgg caacatcatg accgtggaga acgcccagga catcgtcgag
     9721 gaggtgctcc tcgattcccc ctaccggaag accgccaagg cctacatcat ctaccgggac
     9781 cagcacgccc gcctgcgcga aatcacccag aagatggagg tggacctggt cgaccagtac
     9841 ctgaagaaga aggactggaa gatccacgaa aacagcaata tggattactc cctccagggg
     9901 ctcaacaact acatctcctc ggaggtcagc aaggtctact ggctcaacga gatctattcg
     9961 ccggagatcc gcaaggccca cacggacggg gatttccaca ttcacgacct ctccctcctg
    10021 tcggtctact gcgtcggctg ggacctctac gacctgttgt tcgagggatt ccgcggcgtg
    10081 tccgggaagg tggagagccg tccggcgaac catctgcgga gcgccctggg gcagatcgtc
    10141 aacttcttct acaccctgca gggcgaggcg gcgggggccc aggccttttc gaacttcgac
    10201 accctgctcg cccccttcat ccggtacgac ggcctgaatt acagcgagat ccggcaggcc
    10261 ctccaggagt tcatcttcaa catcaacgtc ccgacccggg tggggtttca aacgccgttc
    10321 acgaacgtga ccctcgacgt gacggtgccc gtctactacg cggaccagcc cgtcctcgtc
    10381 ggcggaaagc tgcagaagga gacctacggt gcgtttcagg aggagatgaa cctcttcaat
    10441 cgggccttcg tggaggtgat ggcggagggc gacgcgaagg gacgggtctt cacctttccc
    10501 atcccgacct acaacgtcac ccgggagttc gactgggagg accccaacct cgccggtctt
    10561 tgggagatga cggccaagta cggcgtcccg tatttttcca acttcatcaa ctccgacatg
    10621 aagccggagg acgcccggag catgtgctgc cggctccgca tcgacaaccg ggagttggag
    10681 aagcggggcg gcgggctgtt cggctcgaac cccctgaccg ggtccatcgg cgtcatcacc
    10741 atgaacatgc cccgcatcgg tcatctctcc cgctcggaag acgaatacct ccagcgcctg
    10801 gagcggctga tgggaattgc ccgggagagc ctggagacca agcgcaaggt cctggaagcc
    10861 ttcaccgacg ggaacctgta cccctacacg aagtactacc tccggaaggt caaggagcgg
    10921 ttcggcgagt actggaagaa ccatttttcc accatcggcc tggtggggat gaacgaggcc
    10981 tgcctgaacc tgctcgggaa ggatctcgcc agccccgagg gccagcggtt cacgaagcgg
    11041 gttctcgact tcatgcggga acggctggtt cacttccagc gcgaaacggg caacaactac
    11101 aacctggagt ccacccccgc cgaaggcacc agctaccgcc tcgcccggat cgacaaggaa
    11161 aagtaccccg agatcctctg cgccaacgaa tcgcgcctcc gggaggcaca ggcggaaccc
    11221 ttctacacga actcgagcca gttgcccgtg aattacaccg acgacatctt cgaggccctg
    11281 gacctgcagg acgagatcca gtcgaagtac acggggggga ccgttttcca taccttcgcc
    11341 ggagagcgcg tggacgatcc ggaggccgtc aaggccctgg tccgcaaggt ctgtacgcgc
    11401 taccacctgc cctacctgac ctttacgccc accttcagcg tgtgcccctc ccacgggtat
    11461 ctgaaggggg agcaggaggc ctgtccggca tgccggatgc cgtgcgagat ctattcccgg
    11521 gtggtggggt acctgcggcc gatccggcag tggaacaagg gaaagcagtc ggagttccag
    11581 ctgcggaggg aataccagtt ctgagcggca tatgccggag gcggtggcgg ccatgaatat
    11641 cggcggcttc cagaaggtgt ccctgatcga ttatccggga cggatcagcg cggtgatttt
    11701 cacccagggc tgcaacttcc gctgtccctt ctgccacaac ccggagctcg tcgattccgc
    11761 acgattcggt ccgaccctga gcgaggcgga gatcttcgcc tttctggaaa aaaggcgggg
    11821 cacgctggac ggggtgtcga tcacgggtgg cgagccgaca ctccagccgg acctcgcggc
    11881 gtgcgccacg cggctgaagg gcatgggttt tctggtgaag atcgacacca acggctcccg
    11941 cccggaggta ttgcgcgacc tgatcgaccg gcggttaatc gatgccgtcg ccatggacgt
    12001 caagggatcc ctgaagaaat atgaccgact gacgggtgtc cggaacagcg gccggacggt
    12061 cgaagagagc atccgcgtga tccttgcgtc gggcctgacg cacgaattcc gcacgaccgt
    12121 ggtgccgtcc ctgctgacgg aacgggactt cctcgagatt gggagactcg tggaaggggc
    12181 cgcctccttc gtcgtgcaga cctacgtgcg gacgaagcag ctcgatcccg ccgtgccccc
    12241 gccgggggcc gaaccgccgg acctgaacaa atgcgcggcg cgggtggcgc cgcacgtgag
    12301 ccgggtccgg atccgctgaa atccgttcag atttacttct tcccctgctt ttggtatcct
    12361 gccattcgga gccccgtccg gggccgtttt gccgttccgc gcggcgcgga agacgggagc
    12421 tttgcgaaca ggggagggga cggatcatga gaacatccac aacgcacagg cagaatgccg
    12481 accgggcgat ggtcaaggcc gggccggcca atacccaggc cgtcctggag gcggctgcgt
    12541 ggcgggctga agcctgcaag atccggaaga tcctcatcgc cacgtgcagc ggccggacgg
    12601 cgctcgccgc cgttcaggcg tttcccccgg aaaccgagat catcgcggtg acccatgtga
    12661 cggggtttgc caagccgaac caccaggaac tgacgccggc caaccgccgc cggctggaag
    12721 agaagggcgt tgcgatcttc accggccagc atgccttcgg gggggtgggc cgggggatcc
    12781 ggaaccggct cggtgcctac caggtggatg aaatcatggc cttcaccctg cgcacgttcg
    12841 gccagggga
//
LOCUS       DAOOLJ010000053        12703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5462_length_12703_cov_14.337840, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000053 DAOOLJ010000000
VERSION     DAOOLJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5462_length_12703_cov_14.337840"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..2096)
                     /locus_tag="PLW16_08135"
     CDS             complement(135..2096)
                     /locus_tag="PLW16_08135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPB70914.1"
                     /translation="MIDYDKALNEEQRRVVMEGDGPLLVIAGAGSGKTRALTYRVARL
                     IETGVLPARIMLATFTNKAARSMLARVQSLVGDDLRPLWGGTFHHLANRTLRRHASRL
                     GYEPGFTIADSEDARQLINACIAESRAGRSGEKFPKGDVIREVISLSVNTGRDMAEVI
                     LQRYPFFSHLTEDIGDVAVRYARRKRKEQIMDFDDLLLNWRRLLSEQPDLSERYAERF
                     LHILVDEYQDTNRLQAEIIDLLAARHRNVMVVGDDSQSIYSFRGANFANIMRFPERYP
                     DCRIFKLETNYRSTPEILHLANLSIENNENQFAKTLRPIRRTGLKPIVVPLKSDIQQA
                     NFVAQRLLELIGEGLPLHELAVLYRAHYHSMNVQMELTRRGIPFEIRSGIRFFEQAHV
                     KDVTAYLRVVVNPLDEPAWKRILGLYPKVGKVTADKVWRYLSAREDPLRAVEEAGFPA
                     LASKGAQPGLAALQKTLQALLASERTPAAMIDVVLERGYQAFLEQQYTDMAARREDLE
                     QLANFSGRFEGLEDFLAELSLLTQTTDEGESDRENGRGGRVVLSTIHQAKGLEWSVVF
                     LISCADGMIPLARCLKEEEGEEEERRLFYVASTRAKDQLYLSYPLLDYQRGSGYDVLA
                     PSRFISEIMPSSRRLKDSPFEQWDVGYED"
     gene            complement(2106..2756)
                     /locus_tag="PLW16_08140"
     CDS             complement(2106..2756)
                     /locus_tag="PLW16_08140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPB70915.1"
                     /translation="MKIILLGAPGAGKGTVAKLLTEHDGSVQVSTGDILRAAVKEGSE
                     LGKEAKAYMDRGDLVPDALIMRMMEVRLQGEDCARGFILDGFPRTIPQADDLQVLMKK
                     LGIRLDFVVNLEVPRDVIMDRLTTRRTCANPDCQEIYNVKSNPPTAEGKCRKCGTDVI
                     QRADETEEAIGNRLKTYQDKTAPLIAYYEKAGLLKNFLSLNSRETVENVKLALKGA"
     gene            complement(2856..3539)
                     /locus_tag="PLW16_08145"
     CDS             complement(2856..3539)
                     /locus_tag="PLW16_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB70916.1"
                     /translation="MIRRYRYSLLQKIQTLLCWIIRIYSSTFRLTVENETPWINHLEA
                     GGRVLLCVWHQQFFAAIRYFKIYERYQPSLMISQSHDGDVIARVAIQQGWHPVRGSSS
                     RDGGKALKEIIERLNRFRLAAHIVDGPRGPAGVVKPGAVRIARATDAAVVPFYTTAER
                     AWYFRSWDRFMIPKPFARVTPCFGEMIHFPADIGEEGFEAQRAELEEVMHSDLISPEN
                     RTRRPTTSR"
     gene            complement(3567..4514)
                     /locus_tag="PLW16_08150"
     CDS             complement(3567..4514)
                     /locus_tag="PLW16_08150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPB70917.1"
                     /translation="MKKIATLCVLLTMAGLMACATTDSSRHREGDNTGQDVAMTVEDE
                     KRLTEEALPQMRKDYPAAQNAELQKYIDGLGQKIVKANKLDGQPYHYTFTVVDVENVN
                     AFAMPAGQVFVTAPLIAMASNEAELAGVVSHEIGHITARHTAERMYAMEKAQNKTWIY
                     SAGGGLVGAAAGYGLGRVLCKEGDTACLATAAVIGVAAGAAGGLLVQKYTFMANSRED
                     EMEADRIGFKLAVGAGYDKDQVGKFYEKLLDAEKAGRSGGGVLRSLTDAMSTHPPSEE
                     RVKQMNELAGNQPASRSALVTTPAFNRARQIAQGMQKNR"
     gene            complement(4568..4933)
                     /locus_tag="PLW16_08155"
     CDS             complement(4568..4933)
                     /locus_tag="PLW16_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70918.1"
                     /translation="MKRSQRAEKSPKDSPAFAQMERMFMGIYNQPLLQAMVGLRATDA
                     SPRRQPGEAPGPGGVAFLNCRPIPEPSWKGRKEGEFQQPLNSRVVSNGKEIDEEKAKF
                     ACVAKDRSERIFVEHCCKL"
     gene            5130..6578
                     /locus_tag="PLW16_08160"
     CDS             5130..6578
                     /locus_tag="PLW16_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HPB70919.1"
                     /translation="MLKKAFWGLVALVCLLVVTIVAAGIILYKTVDKTFIESHLSATL
                     HRQVTIEKIDVSLFSIVSGIEAERIAVSNFKTPETLAALAGHPVDEKDLFADLEAFRF
                     KLKFLPLLKRQVEIQELLLIRPVVRLVKDKRGRLNIDDLIRSKKKPLDDRKPWKEDSA
                     PPLSADNLPVAVAISEIGLKDARITYHDAKYNQTFQAYNLTALLQDIRIDPKALAQND
                     EMRVKLDLGVKTLAPLKTGSVQSFDLTLGADGKVIPFDLQTRLLNPEALVRLSLPSGK
                     VTGLQVFNALTKIPILGDYLGSQLSFLKGTQRWSASTESGMNLRYKGDQVEIKSGRLD
                     LAETKILFDGGLNLASKAIKMNLNLTLSQDLNDGIQTSLAKKFDAAIKRPDVRKYVDP
                     SRLAMAAMEPLLNDDGRIALGTSVNGTTEKPRVKLVQPQLRSLDVVIRDSMTSVATEA
                     GKEAVRGAVKEHLNENQQQLLNDLGGLLKRKE"
     gene            complement(6541..7812)
                     /locus_tag="PLW16_08165"
     CDS             complement(6541..7812)
                     /locus_tag="PLW16_08165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPB70920.1"
                     /translation="MNRKRLSAAAMICALMLALDCPVAAGGLSPCLETVDPRGAVLVT
                     DGGGDILVEQNKVTQYVPASTLKLLTALAAMHHWGLSYRFRTEFYMDADRNLKIKGYG
                     DPFLVTEAWEEIARALSTNVRGFHHLILDHSYFAPGIDISGRGESTNPYDAPPGALCA
                     NFNTVYIDPDTKGRIADMEPQTPLSRAAGARFQAMGLKKGRYTFSHDELEITRHAGEV
                     AVYFMKKEGIDMAGDIFFGPVQPRDTLVYAHESPFTLEEVIRRMMEFSNNFVANQIFL
                     GLGAHVYGSPATLEKGIRVIERYLEDELGLTDVHIVEGSGLSRENSLSASEMDGVLIR
                     FEPYRHLLKTKDGVFHKTGRLEGIRTRVGYVETENGNPCRFVVFLNKGRSDIDTLVRC
                     IKKQCQRRLEDSRASGEPDAIPSASTAHPDR"
     gene            complement(7885..8694)
                     /locus_tag="PLW16_08170"
     CDS             complement(7885..8694)
                     /locus_tag="PLW16_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPB70921.1"
                     /translation="MEEVFVKGQELVALYGLKAIAALIILILGRWVAKGVQKLTARIL
                     RRHSVDPAIVSFTESLVYVAMMMFVILAALAQLGIQTTSFIAVIGAAGLAIGLALQGS
                     LANFAAGFLMLIFKPFRVGDYVDGGGSSGSVEKIQIFTTQLVTVDNKVVIIPNSKMMG
                     DNIVNYSAKPTRRVDLDFRVSYGDDIDKARKAIQAVLEKDRRILKDPGAAILVKQLAE
                     SSVVFQLRAWVKTGDYWGVYFDLIEAVKKEFDMQGISIPFPQRDVRVFTVK"
     gene            complement(8755..10893)
                     /locus_tag="PLW16_08175"
     CDS             complement(8755..10893)
                     /locus_tag="PLW16_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB70922.1"
                     /translation="MTPSERIEAVKAAGHDALTEAEAKRFLAAYGIPVVAELTANDAE
                     EAAERADAIGFPVVLKGLGAKLTHKTERGLVRLNLKDRTDVTRAAGDIAVAAGRDLEG
                     FLVQPMLAGKREFVAGMIRDERFGPVIMFGLGGVYTEALRDVTFRFAPLDQAESGRMP
                     GELKSSALLGDFRGETAVDRSQIVRVLTGLSRLALEHPEVAEIDINPLLIGPDGRVCA
                     VDALVVLGGSVLPDSAPRDLSFLDTMLAPRSIAIIGARRSRNGGWQNLMRVIAGMGYT
                     GRLYPINPGAAEIEGIRAYPSLRELPEPVDLVIVAVAATMVPAALEECIATGNRNVHI
                     FAAGFKETGEEEGLRLQREIEEIAIRGGLNVMGPNCMGLYNPALRLATWRSAPTRSGD
                     VAFVSQSGGHAGDLAALAERAGVYFSKAISYGNALTLDSTDFLEYLKHDDQTRIICLY
                     LEGIRDGRRLSKQIAEINPRKPVIIMKGGLTESGARAVASHTGSLAGGPHLWETLFRQ
                     TGAVRVDSLEEMADVVLAFRNLGKPAGRRVAVIGTGGGIGVWAADAIARAGLELPILG
                     EKTQAEFRAFIPEAGNILRNPIDAGIAFTDLGLLERALRIISADPLVDAIVITIHVDW
                     TFNFDEGRHIDRLAHYLAGPAREHTAGKPYLVSWQRYRTLPGIVAAADRLYETLINGG
                     VPLYEGLPRAASALSRVVVYYEFQRNRSPG"
     gene            complement(11282..12565)
                     /locus_tag="PLW16_08180"
     CDS             complement(11282..12565)
                     /locus_tag="PLW16_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70923.1"
                     /translation="MSAKPTPEELTKKIKQLEDELRKAVETAEALRESEAKNRLLLEK
                     MTDTVWTLDMNMKPTYVSPATVEVLGYAPEERLTQHLSEMVTPETYARISELLARELQ
                     KERDGTGDPDRTILLEMEYYHKKGGTVWLENKVRAIRADDGRIVGIHGVSRDITQRKR
                     TEEALRTSEERFRQISNLMTDIIYSCVTDAHGVFSIDWMSGNTETITGYTIDEIKAQS
                     CWQFLVTEEDLPLFETHVTGLSPGQSMSVDLRIRHKDGMISWLTSHAECIEDAKFPER
                     RRLYGGLVDVTNRKQTEEALKNANSQLEAANRELGHAYAWMRDKKDQLKRQLVREEMG
                     ILLDTGGSIEGVSEKVMEYLGKSRDKLIGQGIRELIKEEYHDDFFRALRQAWMGPSDD
                     VFVEFLDVRDRAKIFDVKLTRMTLSGARLVLMTLR"
     gene            complement(12612..12699)
                     /locus_tag="PLW16_08185"
     tRNA            complement(12612..12699)
                     /locus_tag="PLW16_08185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12663..12665),aa:Leu,seq:taa)
BASE COUNT         2426 a         3941 c         3633 g         2703 t
ORIGIN      
        1 gcgtggacat caagttcgaa gactgggtca agcgggtcga agacatcgcg gaagcaacgc
       61 agatcgccct taaggccgaa tatgaagcac gcgccaaatc cgaccgctgg gtctgagtca
      121 ccggcgcggc ggaatcagtc ctcgtaaccg acatcccact gctcgaacgg gctgtccttg
      181 agccgtcggg acgacggcat gatttcgctg atgaagcggg agggtgcgag gacatcgtag
      241 cccgatcccc gctggtaatc gagcagcgga tagctcaaat acaactgatc cttcgcccgg
      301 gttgaagcca cgtagaagag ccgccgctcc tcctcttccc cctcctcctc cttcaggcag
      361 cgggcgaggg ggatcatccc gtccgcgcat gagatgagaa aaacgacgga ccactccagg
      421 cctttggcct gatggatggt gctcagcacc acccttcccc cccggccgtt ctcacggtcg
      481 ctctctcctt cgtccgttgt ctgggtcagg agggatagtt ccgcaagaaa atcctccagc
      541 ccctcgaacc gtcctgaaaa atttgcgagc tgctccagat cctcgcgacg cgcggccatg
      601 tcggtatact gctgctctag aaaggcctga taaccgcgtt cgagaacgac gtcgatcatc
      661 gccgccgggg tccgttccga ggcgaggagg gcctgaaggg ttttctgcag ggcggccagc
      721 cccggctggg ctcccttcga cgcaagcgct gggaaccccg cctcttccac tgcccgcaac
      781 gggtcctccc gtgcggaaag gtaacgccag accttgtcgg ctgtgacttt tcccaccttc
      841 gggtagagcc cgaggatgcg cttccaggcc ggttcatcga gaggattgac caccacccgg
      901 aggtaggccg tcacgtcctt gacgtgggcc tgctcgaaaa aacggattcc ggagcggatc
      961 tcgaagggaa tgccgcgccg cgtaagctcc atttggacgt tcatggagtg gtagtgggcc
     1021 cggtacagga cggccagttc gtgcagcggg agtccctccc cgatgagttc gagcagacgc
     1081 tgcgcgacga agttggcctg ctgaatatcg ctcttgagcg gaaccacaat gggtttcaag
     1141 cccgtccggc ggatcggacg cagcgttttg gcgaactggt tctcgttgtt ctcgatgctg
     1201 aggttcgcca gatgaaggat ttccggcgta ctacggtaat tcgtttctag tttgaagatg
     1261 cggcaatcag gatagcgctc cgggaagcgc atgatattgg cgaaattcgc cccgcggaag
     1321 gaatagatgc tttgcgaatc gtccccaacg accatgacgt tccggtgacg ggcggcgagc
     1381 agatcgatga tctccgcctg gaggcgattc gtatcctggt attcgtcgac caggatatgg
     1441 aggaagcgct cggcgtaccg ctccgacagg tccggctgtt cgctgagcag gcgccgccag
     1501 ttcaggagta gatcgtcgaa atccatgatc tgctccttcc tcttgcgccg ggcataccgg
     1561 accgcgacat cgccgatatc ctccgtcaga tgtgaaaaga agggataacg ctgaaggatg
     1621 acctccgcca tatcgcggcc ggtgttgacg gagaggctga tgacctcacg gatgacgtcg
     1681 cctttcggaa acttctcgcc cgaacgcccg gcccgcgact ccgcgatgca ggcgttgatg
     1741 agctgtcgcg cgtcttcgct gtcggcaatg gtgaagcccg gttcgtatcc gagacgcgag
     1801 gcgtgtctcc tgagggtccg gttggcgaga tgatggaatg tccctcccca gagcggacgg
     1861 aggtcgtctc cgaccagcga ctggacgcgt gcgagcatgg agcgggccgc cttgttcgtg
     1921 aaggtggcca gcatgatcct ggcgggcaaa actcccgtct cgatcagacg ggcaacgcga
     1981 tacgtcaggg cgcgcgtttt gccgctcccg gctccggcaa tgacgagcag cgggccatcg
     2041 ccctccatca cgacgcggcg ctgctcctcg ttcagggcct tgtcataatc gatcatggcc
     2101 cgggatcatg cgcctttcag ggcgagcttg acattctcga cggtctcccg gctgttcagg
     2161 gagaggaaat tcttgagcag acctgccttt tcataataag cgatcagggg cgcggtcttg
     2221 tcctggtaag tcttgaggcg gtttccgatc gcctcttcgg tttcatcggc ccgctgaatc
     2281 acgtcggttc cgcattttcg gcactttccc tccgccgtcg gagggttgct cttgacgttg
     2341 tagatctcct ggcagtcggg attcgcacag gtccgccgcg tggtcaggcg gtccatgatc
     2401 acgtcgcggg gcacttcgag gttcacgacg aaatcgagac gaatgccgag tttcttcatc
     2461 agaacctgca ggtcatcggc ctgcgggatc gtgcggggaa acccgtccag gatgaatccc
     2521 cgggcgcagt cctcgccctg gagacgaact tccatcatcc tcatgatcag ggcatccgga
     2581 accaggtcgc cccgatccat atacgccttg gcttccttgc caagctcgga tccctccttg
     2641 acggccgcac gcaggatgtc gcccgtcgag acctggacgg atccgtcatg ctccgtcaaa
     2701 agtttggcca ccgtgccttt tccggcgccc ggtgcgccca aaagaatgat cttcatgttg
     2761 tcctcctcgt ttctccgcag tccgccttga tggcgtgatc gacgcatttt tgcacgggcg
     2821 ggactataga ggaacttatt cgggccgtca agacattagc gggaagtcgt cgggcggcgg
     2881 gttcggtttt caggggaaat caggtcggag tgcatgactt cctccagctc cgcacgctgc
     2941 gcttcgaatc cctcctcccc aatgtcggcg gggaagtgga tcatctctcc gaagcacggg
     3001 gtgacgcgag cgaagggttt cgggatcatg aagcggtccc agcttctgaa ataccaggcg
     3061 cgctcggccg tggtgtagaa ggggaccacg gccgcgtccg ttgcgcgtgc gatgcggacg
     3121 gccccgggct taacgacgcc cgcgggtccc cgcggtccgt ccacgatgtg tgcggccagc
     3181 ctgaatcgat tcaggcgctc tataatttcc ttcagggcct ttcccccatc ccgcgaggac
     3241 gatccccgca cggggtgcca cccctgctga atcgcgaccc gggcaatcac atccccgtcg
     3301 tgactctggc tgatcatgag cgacggttga taacgttcgt agattttgaa ataacgaatc
     3361 gcggcaaaga actgctggtg ccagacgcag agaaggaccc ggccgcccgc ttctaggtgg
     3421 ttgatccaag gcgtctcgtt ttcaaccgtc aggcgaaacg tcgaggagta gatccgtatg
     3481 atccagcaca ggagtgtctg gatcttttgt aaaaggctgt agcgatagcg tctgatcatc
     3541 gggacgcgcg acatccgatc cttcacttat cggttctttt gcattccctg cgcgatctgc
     3601 cgggcgcggt tgaacgccgg cgtggtgacc agcgcgcttc tggacgcggg ttggttcccc
     3661 gccagctcat tcatctgctt gaccctctct tcgctgggcg gatgcgtgct catcgcatcg
     3721 gtcagactgc gcaggacacc gcccccgctt cggcccgcct tctcggcgtc cagaagcttt
     3781 tcataaaact tgccgacttg gtccttgtcg tatccggcgc ctacggccag cttgaatccg
     3841 atccgatccg cctccatttc atcctcgcgg gaattcgcca tgaacgtgta cttctgaacc
     3901 aagagtccgc cagcggcccc cgccgccacg ccgataaccg ccgcggtcgc gagacaggcc
     3961 gtatctcctt ccttgcagag gactcgtccc aggccatacc ccgccgctgc tccgaccaac
     4021 ccgcccccgg cgctgtagat ccaggtttta ttttgggcct tttccattgc gtacatccgt
     4081 tccgccgtat gccgggccgt gatatgcccg atctcgtgtg aaaccactcc ggcgagctcc
     4141 gcttcattgg atgccatcgc gatcaggggc gccgtcacga acacctggcc ggccggcatc
     4201 gcgaaggcgt tcacattctc gacatcgacc accgtaaacg tgtagtgata gggctgaccg
     4261 tccagcttgt tggccttcac gatcttctga ccgagtccgt cgatgtattt ttgcagctcg
     4321 gcgttttggg ccgcggggta atccttgcgc atttgcggca gggcttcctc ggtcaggcgc
     4381 ttttcgtcct cgaccgtcat ggcgacgtcc tgtcccgtgt tgtctccctc tcgatggcgt
     4441 gaactgtccg tggtggcgca ggccatcaag cccgccatgg tcaatagaac acacagcgtc
     4501 gcgatctttt tcatgattat cctccccttc cctccgttcg gcatccccaa ccttacggaa
     4561 tcgtctatca aagcttgcag caatgttcaa cgaagatccg ttcggatcta tcctttgcta
     4621 cgcaggcgaa tttcgccttt tcctcgtcga tctctttccc gtttgaaacg acccggctgt
     4681 taaggggttg ctggaactcc ccttctttcc ggcccttcca ggacggctca gggatcggcc
     4741 ggcagttgag aaaagcgact ccacccgggc caggcgcctc gcccggctgc cgtctcgggc
     4801 ttgcgtccgt agcccgcaat cctaccatgg cctgcaacag cggctgattg tagatgccca
     4861 tgaacatccg ttccatctgg gcgaaggcgg gtgagtcttt aggggacttt tccgcccttt
     4921 gcgaacgctt catccaaacc ctccctccgt tcaccacaat gggtcccaat atcgcgtcat
     4981 gacacggata agtcaagcag gattgcatac tgatttcgcg ggggaaattt gacattgagg
     5041 tgcgtttcct ctatagttcg tcgaactgtg ccctgggtcg tccggacccg gtccctcttt
     5101 caaccccccc gagaaaggcg gtccgcgtta tgttgaaaaa agcgttttgg ggactggtcg
     5161 ctctcgtctg tctgcttgtc gtcacgatcg tcgcggccgg gatcatcctt tacaaaacgg
     5221 ttgacaaaac cttcatcgaa tcccatttgt cggccaccct tcaccgtcag gtaacgatcg
     5281 agaagatcga tgtgagcctc ttttcgatcg tttccggcat cgaggccgag cggatcgccg
     5341 tctcaaactt caagacaccc gagacactcg cggcgcttgc aggacatccg gtggacgaaa
     5401 aagatctttt cgccgatctg gaggcgtttc gtttcaagct caaattcctg ccactcctca
     5461 agcgtcaggt cgaaatccag gagctcttgc tgatccgtcc ggtcgtccgc ctggtcaagg
     5521 acaaacgcgg ccggctgaat atcgacgacc tgattcgatc caaaaagaaa cccttagacg
     5581 accgaaaacc gtggaaggaa gactccgctc cgcccctcag cgccgacaac cttcctgtgg
     5641 cggttgccat cagcgaaatc ggcctgaagg atgccaggat cacttaccac gatgcgaaat
     5701 acaaccagac cttccaggca tataacttga cggcgctttt gcaggatatc cgcatcgatc
     5761 caaaggcgtt ggcacaaaac gacgagatgc gggtgaagct cgatctgggc gtcaagactc
     5821 tggcacctct gaagacgggg agcgtccaga gcttcgacct cacgctcggc gcggatggaa
     5881 aggtgatccc cttcgatctt caaacccgcc tcctgaaccc ggaggccctt gtccggctct
     5941 ccctcccgag cggcaaagtc accggcctgc aggtgttcaa cgccttgacg aaaattccca
     6001 ttctcgggga ttatctcggg tctcaactct ctttcctcaa agggacgcaa agatggagcg
     6061 cctcgacgga aagcggcatg aatcttcgct acaagggaga tcaggtcgag atcaagagtg
     6121 ggcggctgga tctggccgaa acgaagatat tgttcgacgg aggcctgaat ctcgcctcca
     6181 aggccatcaa gatgaacctg aatctgacac tgagtcagga tctcaacgac gggatccaga
     6241 catcgctggc caagaaattc gacgccgcca tcaagcgccc ggatgtaaga aaatacgtcg
     6301 atccctcccg gcttgcgatg gcagcgatgg aacccctgct caacgacgac gggcgtatcg
     6361 ctctcggcac gtctgtcaac ggaaccaccg aaaaaccgcg cgtgaagctg gtccagcctc
     6421 agctccgatc gctggatgtc gtcatcaggg attcgatgac atcagtggca accgaggccg
     6481 gaaaggaggc tgtcaggggt gcagtgaagg agcatctgaa cgaaaaccag caacaactgc
     6541 tcaacgatct gggtgggctg ttgaagcgga aggaatagcg tccggctcgc cgctggcccg
     6601 ggagtcctcc aaacggcgct ggcattgctt tttgatgcag cggacaaggg tatcgatatc
     6661 cgaccgcccc ttattcagaa agacgacaaa acggcacgga ttcccgtttt cagtctcgac
     6721 ataacccacc cgcgtccgga tcccttccag ccttccggtt ttgtggaaaa cgccgtcttt
     6781 cgttttcagg agatgccgat agggttcgaa tcggatcaag accccatcca tttccgatgc
     6841 cgacagacta ttctcccggg agagaccgga tccctccacg atatggacat ccgtcagccc
     6901 aagttcgtcc tcgaggtaac gttcgatcac ccggattccc ttctccaggg ttgcgggcga
     6961 accgtacaca tgggcgccca ggccgaggaa gatctggttg gcgacgaagt tgttcgagaa
     7021 ttccatcatc cgccggatca cctcttccag agtgaacgga gactcatggg cgtaaaccag
     7081 cgtatcccgg ggttgcaccg gaccgaagaa aatatccccg gccatgtcga tgccttcctt
     7141 tttcatgaaa tacacggcga cctcgccggc atggcgcgtg atttcgagct cgtcgtggct
     7201 gaacgtgtaa cgaccctttt tcaggcccat ggcctggaat cttgcgccgg ccgcccgcga
     7261 caagggtgtt tgcggctcca tatcggcgat ccgacccttc gtgtccgggt cgatatagac
     7321 cgtattgaaa ttcgcgcaca aggcaccggg cggggcatcg tagggattcg tggactctcc
     7381 cctccccgaa atatcgatgc ctggagcaaa gtaagaatgg tccagaatca gatgatggaa
     7441 cccgcgaacg tttgttgaca gcgccctggc gatctcctcc catgcctccg tgaccaggaa
     7501 ggggtccccg tagcccttga tttttagatt ccgatcggcg tccatgtaga attccgtccg
     7561 gaatcggtag gacaaccccc aatgatgcat cgctgcgagc gccgtcagca gcttgagcgt
     7621 ggacgccggg acatattggg taaccttgtt ctgttcgacg aggatgtcgc cgccgccatc
     7681 ggtgacgagg acggccccac gcggatccac ggtctccagg cagggcgaaa gcccgcccgc
     7741 ggccaccgga caatcgaggg ccagcatcag agcgcagatc atggccgccg ccgacaggcg
     7801 tttccggttc attgcccgat tcgctcgtat gcgtatcgac ccatgcactt tccgcatgtc
     7861 aggtagaagc gtcacgactt gcactcattt cactgtgaag acacgaacat ccctctgcgg
     7921 gaatggaatg gagatgccct gcatgtcgaa ttcctttttc acggcctcga tcaggtcgaa
     7981 ataaacgccc cagtagtcgc cggtcttgac ccaggccctc aactgaaaga cgacgctgct
     8041 ttcggcgagt tgcttgacca gaatcgcggc tccgggatcc ttcagaattc tgcggtcctt
     8101 ttcgagcacg gcctgaatcg cctttctggc cttgtcgatg tcatcgccat agctcaccct
     8161 gaaatccaga tccacccttc gggttggctt ggccgaataa ttgacgatgt tgtcgcccat
     8221 catcttcgag ttcgggatga taaccacctt gttgtccacg gttacgagtt gggtggtgaa
     8281 aatctggatt ttctcgacgg atcccgatga gccgccccca tcgacataat ccccgaccct
     8341 gaaaggcttg aagatcagca tgaggaatcc ggccgcaaaa ttcgccaggg acccctgaag
     8401 ggccagaccg atggccaggc ccgcggcgcc gatgaccgcg ataaaggacg tcgtctgtat
     8461 cccgagctgg gcgagcgccg ccaggatgac gaacatcatc atcgccacgt agaccaggct
     8521 ctccgtaaat gaaacaatgg cgggatcgac gctgtgcctt cgcaggattc gtgccgtcaa
     8581 cttctggaca ccctttgcaa cccatcggcc gaggatcagg atgataaggg ccgcaatcgc
     8641 cttcagtccg tagagcgcta ccagttcctg ccccttcaca aagacttctt ccataaatca
     8701 cctccattgg gttccgggcc aataccctat cccggtcttt ccatcaacca cccgtcatcc
     8761 gggtgatcta ttccgctgaa attcataata cacgacgacc ctgctgaggg cggaggccgc
     8821 ccgggggagg ccctcgtaca ggggaacgcc cccgttaatc agggtttcgt aaagccgatc
     8881 ggcggccgcg acgatgccgg ggagggttcg gtaacgctgc cagctgacga gatacggttt
     8941 tcctgccgta tgctcccgcg ccggtccggc gaggtagtgc gcgagtcgat cgatgtgcct
     9001 gccctcgtcg aaattgaagg tccagtcgac gtggatcgtg atgacaatcg catcgacgag
     9061 cggatccgcc gagatgatcc tgagggcccg ctccaggagc cccagatccg tgaaggcgat
     9121 gccggcatcg atgggattgc ggaggatatt ccccgcctcg gggatgaagg cgcggaattc
     9181 cgcctgagtt ttctcgccga ggatcgggag ttcgagtccc gcccgggcga tggcatccgc
     9241 cgcccagacg ccgatgccgc cccccgtacc gatcacggcg acgcgtcgtc ccgccggttt
     9301 tccgaggttc cggaaggcca ggacgacatc ggccatttcc tccagggaat cgacccggac
     9361 cgccccggtt tggcggaaga gggtctccca gaggtgcggt cctccggcca gggagcccgt
     9421 gtgggaggca acggcgcgcg cgccggattc ggtcaggccg cccttcatga tgatgacggg
     9481 cttgcgggga ttgatctcgg caatctgttt gctcagtcgt cggccgtcac gaatcccctc
     9541 aagatacagg cagatgatgc gcgtctggtc gtcgtgcttg aggtattcga gaaaatccgt
     9601 gctgtccagg gtcagggcat tcccgtagct gatggccttg ctgaaataga cacccgcacg
     9661 ctcggccagg gcggccaggt cgcccgcatg accgccgctc tgcgagacga aggcgacgtc
     9721 gccgctccgt gtcggggcgg aacgccatgt ggcgaggcgc agggcgggat tgtagagtcc
     9781 catgcaattc gggcccatga cgttcagtcc gccgcgtatc gcgatctcct cgatctccct
     9841 ctggagccgc agtccctcct cttccccggt ttccttgaag ccggccgcaa agatgtgcac
     9901 gtttcggttt ccggtggcga tgcactcctc cagggccgcg ggcaccatcg tcgccgcgac
     9961 ggcgacgatc accagatcca cgggctccgg gagttcacgg aggctcgggt aggcccggat
    10021 tccttcgatc tccgctgccc cgggattgat cgggtaaagc cgccccgtgt agcccattcc
    10081 ggcgatgacc cgcatgaggt tctgccagcc gccgttcctg gagcgccggg cgccgatgat
    10141 ggcgatgcta cgtggggcga gcatcgtatc caggaaggaa aggtccctcg gggcgctgtc
    10201 gggaaggacg gatccgccga gcacgacgag ggcgtcgacc gcgcagaccc ggccgtccgg
    10261 tccgatcaag agggggttga tgtcgatctc cgcgacttct ggatgctcca gggccagtcg
    10321 ggaaagaccg gtcaacacgc ggacgatctg gcttcggtcg acggccgtct ccccgcggaa
    10381 gtcgccgagc agggccgaac tcttcaactc gcccggcatg cgccccgact ccgcctggtc
    10441 cagcggggcg aagcggaagg tgacgtcccg gagggcctcg gtgtagaccc cgccgagacc
    10501 gaacatgatc accggtccga accgttcgtc gcgaatcatg cccgccacga attcccgctt
    10561 ccctgcgagc atgggctgga cgagaaatcc ttcgaggtca cggccggccg ctacggcgat
    10621 gtcccccgcg gcccgggtga cgtctgtccg atccttcagg ttcaggcgga cgagcccccg
    10681 ctccgtttta tgggtcagtt tggcccccag tcccttgagg acgaccggga acccgatggc
    10741 gtccgcgcgt tccgccgcct cctccgcgtc gttcgcggtc agttcggcaa ccacgggtat
    10801 gccgtaggcg gcaagaaacc gcttcgcctc cgcctccgtc aatgcgtcgt gcccggcggc
    10861 cttcactgct tctatccgtt cactcggcgt caacctatcc tctcccccct caaacaccaa
    10921 tggctccctc ccttctcgtt tcgtaggtca gcctgaagac cgtgtcggtc atccaacgct
    10981 tgaacgactc actgtccctg agttgtttga aaacgattgc catccttcct ttgaatgtaa
    11041 ggcgaaatct cagtggcatc ggtcatttcg atttcggtat gcataccacc ctgccccgtg
    11101 ttgtcaagac aaaatgactg aaaaaacgga tgcctgctgc tggtgttgtg ccggctcaag
    11161 attttgggga gtttgatatc gggaagtgtt gcgtgcctcc tgcgaggatg tcgtcgcatc
    11221 accgagatgc gagctcttgc ggaattcagc ggaattattc gttatggcaa aggaaggata
    11281 ctcagcggag cgtcatcagg acgagccggg cgccggacag ggtcatccgt gtcagcttca
    11341 catcgaaaat cttcgccctg tcccggacgt cgagaaattc aacgaagaca tcgtcggacg
    11401 gccccatcca ggcctgccgc aacgcccgga agaagtcgtc gtggtactct tccttgatca
    11461 attcccgtat gccttggccg atcagcttat cgcgcgattt tccaagatac tccatgacct
    11521 tctccgaaac gccttcgatc gatccaccgg tgtccaacag gattcccatc tcttcccgga
    11581 ctagttggcg cttcaattgg tccttcttgt cccgcatcca ggcataggca tgccccagtt
    11641 cccgattggc cgcttcaagt tggctgttcg cgttcttcag ggcctcctcg gtttgtttgc
    11701 ggttcgtgac gtccaccaac ccgccgtaga gacggcgcct ttccgggaac ttggcatctt
    11761 cgatacattc agcatgagaa gtcagccagg agatcatccc gtccttatgg cgaatgcgaa
    11821 gatcgaccga catcgactgc cccggggaca gtcccgtgac atgcgtctca aagaggggga
    11881 gatcttcttc cgtcaccaga aattgccagc aggattgcgc cttgatctcg tcaatcgtat
    11941 agcccgtgat ggtttcggtg ttccctgaca tccagtcgat cgaaaaaaca ccatgcgcat
    12001 cggtcacgca ggagtagata atatcggtca tgaggtttga gatctgtcga aatcgctctt
    12061 cactcgtccg aagcgcctcc tccgtccttt ttcgctgagt gatatctctc gacaccccgt
    12121 gaatgccgac gatgcggccg tcatccgcgc gaatcgcgcg aaccttgttt tcgagccaga
    12181 cggtcccgcc cttcttatga taatactcca tctccagcag gatcgtccgg tcggggtcgc
    12241 ctgtcccatc acgttccttc tgaagttccc gggcaagcag ttcgctgatc cgtgcatacg
    12301 tttccggcgt caccatttca gacagatgtt gcgtcaacct ttcttccgga gcgtacccca
    12361 ggacctcaac cgttgccgga ctcacgtaag tcggtttcat gttcatgtcg agcgtccaga
    12421 ccgtgtcggt catcttttcc aaaaggaggc ggttcttcgc ctcgctttct cgcaaagcct
    12481 ccgcggtctc cacggctttc ctcaattcat cttccagctg cttgattttt ttcgtcagtt
    12541 cttccggtgt gggttttgcg ctcatcgggt ggttcccctc ttgcgaaccc attgggacct
    12601 tgaatgtcgc ctggtgcctg gggcgggaat cgaacccgca cagccgcgag gaccgaggga
    12661 ttttaagtcc cttgcgtcta ccaattccgc cacccaggct ttg
//
LOCUS       DAOOLJ010000054        12569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5544_length_12569_cov_19.068563, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000054 DAOOLJ010000000
VERSION     DAOOLJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_12569_cov_19.068563"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLW16_08190"
     CDS             <1..436
                     /locus_tag="PLW16_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70924.1"
                     /translation="ADLDAARKILDGAEAQMKEKNYKEAKQAYLDAKAAFERATAAVA
                     AGKQAAADEANAAVTALDEAWANLETTAKTVERKLKDQKDLWTADIATFTESLKTVKE
                     GIATDPAGAKAKIAELQSIVDKWDAAFKELAAAAPLKPAQKK"
     gene            821..1444
                     /locus_tag="PLW16_08195"
     CDS             821..1444
                     /locus_tag="PLW16_08195"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB70925.1"
                     /translation="MELILVRHGETAWNREGRVQGLSDIDLSDVGVHQARRLALSLRD
                     HPIHAIYASPLIRARKTAEIINQYHDAPIRLEPGLMEMDQGDFEGLAYSELMACERGF
                     LQSWIADPASVRMPHGESLDELQARAWPVIDAILHQSADALVVAHSFTIASILCRIKR
                     ISLSDFRSVHVDPASRTLVRFSNGSGEIVVLNDRTHLNDREPSARSA"
     gene            1482..2651
                     /locus_tag="PLW16_08200"
     CDS             1482..2651
                     /locus_tag="PLW16_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPB70926.1"
                     /translation="MKPDTTSWNTLIRTARLALLLLCVFLSACVAATERVQDPPSSSV
                     RCIPDFPDRDGWYGGDGAYSIPLDDRRVLWLFGDTFVSDQTGRRDRTGMKVVLGTTLA
                     VSTCSADGQFQIRYWLKKKDGEFVSSFGENEWLWPQDPFMVDRILHVPLISVEADPRM
                     PGVFKFRIAGHKIARIRNYAEADPNRWTADYVDLTPGIPEGVEAFATTSVVFGDHVYF
                     YPLYRGAGVLGNILARIPANRLDDPAPAIEYWTRDGRWETGLDPATVKVVLDAAVSEM
                     SVRHHPDDGRWVAVYLSVENNGRRMLYQTADAPEGPWTEPKILIGSIPEVDRSSPRYD
                     PDNFCYAGKEHPEFGREKTLVVTYVCNSFANAEEDASFIRQNLFLYRPVVVTPSR"
     gene            2910..3710
                     /locus_tag="PLW16_08205"
     CDS             2910..3710
                     /locus_tag="PLW16_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPB70927.1"
                     /translation="MEETLSHDMDLRFVTATNSDGRQGKAGSVLALPLALSLAIHVAL
                     GLLLLGEVFRTLPPRLRDASLSPLQVIWVSTSKTATPAPRHSPEVKRTPAAAVTGEAA
                     APASPPAALAGRSATPTLSKAPAQNLTESSTAQPSGIRPGPGTGTAAPPASPPSIGVR
                     VAATGEATALTLPRYRAKDAPMYPALARLRGYEGLVLLAAEVSEQGRVQTIRIKKSSG
                     YAVLDRSAEEAVKSWRFEPGRRMGTPMAMWVDVPVRFVLQDAPAFSDS"
     gene            3780..4385
                     /gene="pyrE"
                     /locus_tag="PLW16_08210"
     CDS             3780..4385
                     /gene="pyrE"
                     /locus_tag="PLW16_08210"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPB70928.1"
                     /translation="MDERKKRLIELILEKSFQYREDPPFTLASGRQSHFYFNCKPTTL
                     DPEGMNLIGEILCDMLADVPVTAAGGLTLGADPLANALAVISFQRRKPVKAFIVRKEA
                     KDHGMKSKIEGNVQSGERVVILDDVITTGGSTITAIEAAREAGLVIDRVITLVDREEG
                     GRENILSHCPVVQSVLTRTEVMDLYHDRAVRPPLDKPSPKG"
     gene            4415..5122
                     /locus_tag="PLW16_08215"
     CDS             4415..5122
                     /locus_tag="PLW16_08215"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HPB70929.1"
                     /translation="MKRFVLPIAVGLFILFGLATTVFPLREDAVVVARFLSPELHRGA
                     PTGWILDRKAGTPVIGFERDGDAYALRLFSDRHSAFGISRPVRVNVREYPYLNWRWKV
                     ARLPKGGDIRKASTNDQASQLYVAFTPVGLQARLNAPVVGYIWDNEAPKGLTVQSPYH
                     LLGKVRYIVLRNKTDELGRWHMEKRNVYEDYKRLFREIKDGEPQGTTQGVQIYINSQN
                     TRSEAEAALGEIYFSKR"
     gene            5240..8524
                     /locus_tag="PLW16_08220"
     CDS             5240..8524
                     /locus_tag="PLW16_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HPB70930.1"
                     /translation="MSLTDTPKRAWIGLIAALALAGIAAAVLYTQLPKLLDPERYRTE
                     ILEAARKHLHRDLTFSRAEFSCRPNPALTVQGLKLTDRDGASPFLSAEKASLHPALLP
                     LLRGRVVLKSLTLDRPCLFVVRGPDGCFNIADLLEGPSTDPVRQWRIRDGQAVFLDRQ
                     AGQNGHVTTLAGVNLRIDDWRRGGKTVLRMESRLSARPRSPASPAFAHAGAAPPGGEG
                     PEPPPATLNLNGTLALAGHDKPLSASRFDGRLRLGGLSLDQFRPYGEAFGVSAAAGRL
                     DLSLTGQGTADRFDVRAEASLAALNVSWPAVFPRPLTPDAVRVRGRLTREPGQWVLDG
                     TRVDLDRLLLEGGFRLHALDTRDPILSARVSIPRLSLAEAKPYIPFGLLPRDTGNFFR
                     KTLCQAVFTDVTGRLQGRLSRIRQMDRGDNARALAVRSRVDRGTLSFGSEIPLFQDVA
                     GQIRLDGTDILFEEMTGRIGASPFRLDGRITGYPLDAPSRYPFTATLNPRPREVQWLL
                     GEAGERLQYSGASVLHLSGDGTTGDYRLTGAWDATAAAYQLPGILTKPAGVSNRIHAV
                     LGFPPGSVRIQDMKFDLAALSATLKGVIPLDRGGRLRLNIRTGALPIEQMAPYVPRLK
                     PYEPSGKIRLNLGARGGAGAETLRWRGRISVADAAFRPPAGLKPVRDLNGALVLTGTG
                     LAGDDLSARLGRTPISASLRLDDWENPTLRVGLRAPRLDLADAGLTHPRRDVSLRDVD
                     LKADYRNDAWQIRNLSATLNQTTLRAQGVIQEKPEPRADLVLSFPFLDIDGMAPLAEI
                     EGGGWTEQSGPRLWTVVLKAESGRNKALTFSNLSARIRYQNDRIDITDGEVDLFGGKA
                     QGEGRIDLRQEGRPAYDARFSLTGADAEQATTALEMPDGFVTGTFSLGGQVQVSGSAR
                     DDLIRTAAGTLTFKAKDGMIRKFAVLSKVFSILNVSQLFKFQLPDMVSGGMPYSRITA
                     RLSLADGVIVTEDLFIRSNAMNISVVGKTDLVKGVTEQTIGVQPLQTVDKVVSRIPVV
                     GWILSDEDGRVLTVYFDAQGPLDDPVVQAVPVQSLSKGLLDAFRNLFELPARLFTDTG
                     EVLLGR"
     gene            complement(8521..9195)
                     /gene="nfi"
                     /locus_tag="PLW16_08225"
     CDS             complement(8521..9195)
                     /gene="nfi"
                     /locus_tag="PLW16_08225"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HPB70931.1"
                     /translation="MIETRHRWDCDPREAVGIQQALRRRLVLADALPGVVRTVAGADV
                     SYDRGSDRFFAAVVVLTYPELVVKEEAHGVGRVTFPYIPGLLSFREGPVMLSALGRLA
                     EPPDLILFDGQGIAHPRGIGLASHLGLWLDRPTIGCAKTRLCGTFDPVGEAPGSRSDL
                     RMDGRVVGTVLRTKRRTNPLFVSPGHRVAVETAAAVVLACCRNYRLPEPTRRAHLLVN
                     RLRREA"
     gene            9539..12064
                     /locus_tag="PLW16_08230"
     CDS             9539..12064
                     /locus_tag="PLW16_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB70932.1"
                     /translation="MHTTASSTPSPENTLGNLNSLMRSLLASIPHAVIGLKDRRIVFA
                     NSAVKTVFGWRPEELIGQTTRILYRSDRDYEEVARRFYPILANRKTYQAEFPCRRKDQ
                     RDITCMLSAARVGSRLRERMIIVTYEDITRRQEDREALRESEQFLKMILSGSPIATFV
                     IGRDHRIMFWNRALEELTKIPARKVVGTRQQWRAFYRSRRPCMADLIVDQAHETIAQW
                     YAGTFSKSNLIEDAYEATDHFPALGEGGKWLRFTAAAIRNSEGQLVGAIETLEDITQR
                     QQAEEALRKAHEHLELRVQERTAALARVSDALQAELAERRRAEEALKQTTDQLSLILE
                     SLPIVSFTCRPRGRYPFTFISSTIEELTGYAPRQFLEKAGFWQDRLHPDDRSRVLAEL
                     KAGMKSGTLHTRYRFQIADDGYRWFSDNRRLITLPDGASQHIVGAWQDITEETRMRQE
                     AELRMQQMIQTHKLTALGEVVTGVAHEINNPVSFIAYNAPLLEEIWSVLEPLLADLGP
                     ERRGGQVGTLSFEEVSRNMREIIAALKISSNRISRVIASLKDFARADEPAKTKPVQIS
                     EVIQGALTIVGGQVRKTVSRIELDVEDNLPLLRGHFQKIEQVITNLLINAHQAMPPGR
                     KGTVRITARHVRSLSALAIEIEDNGRGMEREVLSHLFDPFFTTRRDWGGTGLGLSISY
                     GLIREHNGLIGVLSRPGMGSRFLILLPLNGQSKIELRPAMLCVDHDRGFVSRLKANFV
                     DAVEYPLTSDYRPGDIVAHLLEHPEIDLVLAEIDLPGEGGWAVLDQIKRRFPLLPVIL
                     CSRRPAAGGRTPEDGPRADFILKKPFRIEQLQKMIRETGRQRL"
     gene            12061..>12569
                     /locus_tag="PLW16_08235"
     CDS             12061..>12569
                     /locus_tag="PLW16_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB70933.1"
                     /translation="MRILVVDDETLALTSVQRLLRWRGYPDVEICDSGREAIRKIREE
                     AFDIVILDLLMPEVDGMQVLEATKPYRPETEFIILTAVDDIPTTVRAIRLGAYDYLVK
                     PTENELLLLSVERAYEHKGLVAGLSASVRKAPAEMPAAFADTITRHPKMMSLISYAQV
                     MARGGNPIL"
BASE COUNT         2254 a         4355 c         3987 g         1973 t
ORIGIN      
        1 cgcggacctg gacgcggcca ggaagatcct ggacggagca gaggcccaaa tgaaggaaaa
       61 gaattataaa gaggccaaac aggcgtatct ggacgccaag gccgccttcg agcgggccac
      121 cgccgcggtg gccgcgggca agcaggccgc cgcggacgaa gcgaacgccg ccgtaaccgc
      181 cctggacgag gcctgggcga atctcgaaac cacggcaaaa accgtcgaaa gaaaactgaa
      241 ggaccagaag gatctctgga ccgccgacat cgccaccttt acggaaagcc tgaaaaccgt
      301 caaggagggt atcgccaccg atccggccgg cgccaaggcg aagatcgccg agctgcaatc
      361 catcgtcgac aagtgggatg ccgccttcaa ggaactggcg gcggccgccc cgctcaaacc
      421 ggcccagaaa aaataatccg agccggacat acgaggcatg catcggtcaa ggggcaccgc
      481 cgccgaaaaa ggcgcggcgc cctttttttg tcgccgtccc ggcgacggcg cggccggccg
      541 ccgcggacgc gttttggggt gagccgcgac cacggcggtg gcaaaggcgg agaagggttt
      601 atccatctcc gaggacaggt tccggtaata aaccgcctcg tcttggtccc ccttcgcgtt
      661 catcacgatg gcgtgggtat tgtaataggc ggcctcgtcg tcggtggccc tgaccggaag
      721 gttctccacc atgaaaaaca gccgggcgac ggttgacaca aacggggccg gcggccgttt
      781 cgattgacac cggatcgttc ggttgataag gaaggtccct atggaactga ttcttgtccg
      841 gcacggcgaa accgcctgga accgggaagg ccgggtccag ggcttgagcg acatcgacct
      901 gagcgacgtc ggcgtgcatc aggcccgccg gctcgccctg tccctcaggg atcacccgat
      961 ccacgcgatc tacgccagcc ccctgatccg ggcccggaag acagccgaga tcatcaacca
     1021 ataccacgac gccccaatcc gcctggaacc gggtctgatg gaaatggacc agggggattt
     1081 cgaaggcctg gcctattcgg aactgatggc ctgtgaacgg ggcttcctcc agtcatggat
     1141 cgccgatccg gcgtccgtgc ggatgcccca tggggaatcc ctggacgagc ttcaggcccg
     1201 ggcgtggccg gtcatcgacg ccatcctgca tcaatccgcc gacgccctgg tggtcgccca
     1261 cagcttcacg atcgcgtcca tcctttgcag gatcaagcgg atcagcctgt cggacttcag
     1321 gtccgtccac gtggaccccg cctcccggac cctcgtccgg ttcagcaacg gatcgggaga
     1381 gattgtcgtg ctgaacgacc ggacccacct gaacgacagg gagccatccg cccgctccgc
     1441 atgagcgccc ccgcggaccc ggcaggggac aggcaggccg catgaaaccc gacacgacat
     1501 cctggaacac cctgatcaga acggcccggc tggccctgct cctgttgtgc gtctttctct
     1561 cggcctgtgt cgccgcaacg gagcgggtgc aggatccgcc gtcgtcgtcg gtccgctgca
     1621 ttcccgattt tcccgaccgg gacggctggt acggcgggga cggcgcctac tctatcccgc
     1681 tggacgaccg gcgcgtcctg tggctcttcg gtgacacctt cgtttcggat cagacgggcc
     1741 ggcgggatcg cacagggatg aaggtggtcc tgggaacgac gctggccgtc tccacctgct
     1801 ccgccgacgg gcaattccaa atccggtatt ggctgaagaa gaaggacggg gaattcgtgt
     1861 cgtccttcgg cgaaaacgaa tggctgtggc cgcaggaccc ctttatggtc gatcgcatcc
     1921 tccacgtccc gctgatttcc gtcgaggcgg acccacggat gccgggggtc ttcaaattcc
     1981 gaatcgcggg gcacaagatc gcccggatcc ggaactacgc ggaggccgat ccgaaccggt
     2041 ggaccgcgga ctacgtagac ctgaccccgg gtattccgga gggggttgag gcgtttgcga
     2101 cgacgtcggt cgtgttcggg gatcatgtct atttttatcc gctgtaccgc ggggcggggg
     2161 tcctgggcaa catcctcgcc cgcattccgg caaaccggct cgacgatcca gcccctgcca
     2221 tcgagtactg gacccgggac ggacggtggg aaacaggact ggatcccgcg acggtcaagg
     2281 tcgtcctgga cgcggccgtt tcggagatga gcgtgaggca tcatcccgat gacgggagat
     2341 gggtcgccgt ctatctctcc gttgaaaaca acggccggcg gatgctctat cagacggcgg
     2401 acgcgcccga agggccctgg accgaaccga agatcctgat cgggtccatt cccgaagtcg
     2461 accggagcag cccccgatac gacccggaca acttctgcta cgccggcaag gagcacccgg
     2521 aattcggccg ggaaaaaacg cttgtggtca cctatgtatg caattccttc gcgaatgccg
     2581 aagaagacgc aagctttatc cggcagaacc tcttcctcta ccgccccgtc gtcgtgaccc
     2641 caagccgctg aaccgtctac gcagtcacga gacgcgaaaa atcgcttttg aaaagcaggt
     2701 ctgcccgtcc atcccgcccc ggtgcctgaa ttcggggccg gtcgcgccgg atgaccggcg
     2761 caccgacgga gaggcgctgc ccccgaacag aacgcggccg tcgtggctcg gccggcaaat
     2821 tcgttgacag gggcaagcgt tttccctata ctcccgctgc ccgatccccc gggccgaagc
     2881 gcgccggggg cgcggccgga ttggacccga tggaagaaac cctttcgcac gatatggacc
     2941 tgcgattcgt cacggcgaca aactctgacg gacgacaggg gaaggccgga agcgtcttgg
     3001 cgctccccct cgccctgtcc ttggcgatcc acgtagcgct gggcctgctg ctcctcggag
     3061 aggttttccg aacgctcccc ccccggctcc gcgatgcgag tctgtcgccc ctgcaggtca
     3121 tctgggtttc cacatcaaag acggcgacgc ccgcgccgcg gcactcgccg gaggtgaagc
     3181 gcacaccggc ggcggcagtg accggcgagg cggccgcacc cgcctctccg cccgcggcac
     3241 tcgccggacg ttcggcaacc ccgaccctct cgaaggcgcc ggcgcagaat ctcacggaga
     3301 gctcaaccgc acaaccgtcg ggcatccgtc ccggtcccgg gacgggcacg gccgccccac
     3361 cggcctcccc cccgtcaatc ggcgtccgcg tcgcggccac cggggaggcc accgcgctca
     3421 ccctgccgcg ctaccgggca aaggacgccc cgatgtatcc ggccttggcc cgcctgcggg
     3481 gttacgaagg gctggtgctc cttgccgcgg aggtttccga gcaggggcgc gtccagacga
     3541 tccggatcaa gaaatcctcc ggctacgccg ttctggaccg gtcggccgag gaggccgtca
     3601 agtcctggcg tttcgagccg gggaggcgga tgggaacgcc catggccatg tgggtggatg
     3661 tcccggtgcg gttcgtcctc caggatgcgc cggccttctc cgactcctag gcggcgggcg
     3721 gaaggccgga accctcacga cggcgtcccg gatggcatac gcaacaagga gaccccttta
     3781 tggatgaacg caaaaagcgc ctcattgaac tgattctgga aaagtccttc caataccgag
     3841 aggacccgcc gttcacgctg gcctccggcc ggcaaagcca tttttatttc aactgcaaac
     3901 ccaccaccct cgacccggag gggatgaacc tgatcgggga gatcctctgc gacatgctgg
     3961 ccgacgtccc cgtcacggcc gcgggggggc tcaccctcgg cgccgatccg ctcgccaacg
     4021 ccctggccgt catctccttc cagcggagaa agcccgtcaa ggccttcatc gtccgcaagg
     4081 aggccaaaga ccacgggatg aagagcaaga tcgaggggaa cgttcagtcc ggcgaacggg
     4141 tcgtgatcct cgacgacgtc atcaccacgg gcggttcgac gatcaccgcc atcgaggccg
     4201 cccgggaggc tggcctcgtc atcgaccggg tgatcacact ggtcgaccgg gaagaggggg
     4261 ggcgagaaaa catcctctcc cactgccccg tggtccagag cgtcctcacc cggacggagg
     4321 ttatggacct ttaccacgac cgggcggtcc gtccgccgct tgacaaaccg tccccaaaag
     4381 gctaaagggc gccgatttta tgcatgaggc gccgatgaaa aggtttgttc tgccgatcgc
     4441 ggtcggactg ttcatcctgt ttgggcttgc gacaacggtt ttccccctgc gcgaagacgc
     4501 cgtggtggtg gcccgctttt tgtcccccga gctgcaccgc ggcgcaccga ccggatggat
     4561 cctggacagg aaggcgggca ccccggtcat cggtttcgaa cgcgacgggg acgcctacgc
     4621 cctccgcctg ttcagcgacc gccactcggc cttcgggatc agccgtcccg tgcgcgtcaa
     4681 cgtccgggaa tacccctacc tgaactggcg ctggaaagtg gcccgcctgc cgaagggcgg
     4741 cgacatccgc aaggccagca ccaacgacca ggcgagtcag ctttacgtcg ccttcacccc
     4801 cgtcggtctc caggcgcgtc tgaacgcccc cgtggtcggt tatatctggg acaacgaagc
     4861 ccccaagggc ctgaccgtcc aaagccccta ccacctcctg ggcaaggtcc gctacatcgt
     4921 cctcaggaac aagaccgacg agctcggccg gtggcacatg gaaaaacgga acgtttacga
     4981 ggactacaag cggctgttca gggaaatcaa ggacggcgaa ccccagggga ccacgcaggg
     5041 cgtccagatc tacatcaatt cccagaacac ccgcagcgag gcggaggccg cgctggggga
     5101 aatctatttc tcgaagcgat gagcccgaca acaacccacc cccgggaaag cccccctttt
     5161 tccaccggat gtgctatggt gccctcaggg atgggcatga tccgcctcat tcccggaatg
     5221 aaacccagga tacaccccca tgtccctcac cgacacaccc aaacgggcct ggatcgggtt
     5281 gatcgcggcc ttggctctgg caggcatcgc cgcggcggtc ctctacaccc agttgccgaa
     5341 actgctggac ccggagcgct accggaccga aatccttgag gccgcccgga agcacctaca
     5401 tcgcgacctg acgttctccc gggccgaatt ctcctgccgt cccaatcccg cactcaccgt
     5461 ccaggggctg aagctcacgg accgggatgg cgcctccccg ttcctgtccg ctgaaaaggc
     5521 ctcgctgcac ccggccctcc tgcccctgct gcgcggccgg gtcgtcctca agtccctgac
     5581 gctcgaccgc ccctgcctgt tcgtcgtccg cggcccggac ggatgcttca acatcgcgga
     5641 cctcctggaa ggtccctcga ccgacccggt gcggcagtgg cgcatccgtg acgggcaggc
     5701 tgtcttcctg gaccggcagg ccggccaaaa cggtcacgtg acaaccctgg cgggcgtgaa
     5761 tctgcggatt gacgactggc gtcggggggg aaaaacggtc ctccggatgg aaagtcgctt
     5821 gtccgcccgt ccccggtccc cggcatcccc tgcgtttgca cacgcgggcg cagccccccc
     5881 gggaggggag gggccggaac cgcccccggc taccctgaac ctgaacggga cactggcctt
     5941 ggccggccac gacaagccgc tgtccgccag ccggttcgac ggccgcctgc gactgggggg
     6001 gctctcgcta gatcagttcc ggccctacgg ggaggccttc ggcgtttcgg cggcggcggg
     6061 tcgcctggac ctgtcgttga ccgggcaggg gacggccgac cgcttcgacg tgcgggccga
     6121 ggcgtcgctc gcggcgctga acgtctcctg gccggcggtc tttccgcggc ccctgacccc
     6181 cgacgcggtt cgcgtccgcg gccgtctgac ccgtgaaccc gggcaatggg tcctcgacgg
     6241 cacccgggtc gacctcgatc gccttctgct ggaaggtggc ttccgcctgc acgcgctcga
     6301 cacccgcgac ccgatcctgt ccgcacgcgt atcgatcccc cgcctgagcc tggcggaggc
     6361 gaagccgtac atccccttcg gcctccttcc ccgggacacg ggaaactttt tccggaaaac
     6421 cctctgccag gccgtcttca ccgacgtcac ggggcgtctc cagggacgcc tcagccggat
     6481 ccggcaaatg gaccggggcg acaacgcccg ggcgctggcc gtgcgctcgc gggtggaccg
     6541 gggaaccctc tccttcggtt cggaaatccc gctttttcag gacgtcgccg ggcagatccg
     6601 cctggatggg acggacatcc tgttcgagga gatgaccggc cgcatcggcg cctcaccgtt
     6661 ccgcctggac ggccggatca cgggctaccc cctcgacgcc ccgtcgcgct acccgtttac
     6721 cgcgaccctg aatccccggc cccgggaggt ccagtggctg ctgggggagg caggcgagcg
     6781 tctccagtat tccggcgcat cggtcctgca tctgtcgggc gacggcacca ccggcgacta
     6841 tcgcctgacc ggggcgtggg acgccaccgc cgccgcctat caactccccg gcatcctgac
     6901 caagcccgcg ggggtttcca accggatcca cgcggtcctg gggtttccgc ccgggtccgt
     6961 ccggatccag gacatgaaat tcgatctggc ggccctttcg gccacgttaa agggcgtcat
     7021 tcccctcgac cgggggggac gcctccgcct gaacatccgg accggggcgc ttcccatcga
     7081 acaaatggcg ccgtacgttc cccgtctgaa accctacgag ccgtccggca agatccgcct
     7141 gaacctcggc gcccggggcg gggcgggggc ggaaaccctc cgctggcgag ggaggatctc
     7201 ggtcgcggat gccgccttcc ggccgccggc cggcctcaag ccggtccggg acctgaacgg
     7261 cgccctcgtc ctgacgggga ccggcctggc cggagacgat ctctccgccc gcctgggccg
     7321 caccccgatc tcggcgtccc tgcgcctgga cgactgggaa aacccgaccc tccgggtggg
     7381 tctccgtgcc ccccggctgg atctggccga tgccgggctg acgcaccccc ggcgggacgt
     7441 gtcgctccgg gacgtcgacc tgaaggccga ctaccggaac gacgcctggc agatccggaa
     7501 cctgtccgcc acactgaacc aaacgacgct ccgggcccag ggggtgatcc aggagaaacc
     7561 cgagccccgc gccgacctgg ttctgtcctt cccgttcctc gatatcgacg gcatggcgcc
     7621 tctggcggag atcgagggtg gaggctggac ggaacagagc ggtccccgcc tctggacggt
     7681 cgtccttaag gccgaaagcg gccggaacaa ggcgctgacc ttcagcaacc tctccgcccg
     7741 catccgctat cagaacgacc ggatcgacat caccgacggc gaggtcgacc ttttcggcgg
     7801 caaggcgcag ggggaaggcc ggatcgacct gaggcaggag gggcggccgg cctacgacgc
     7861 ccgcttctcc ctgacgggcg ccgacgcgga acaggcgacg acggctttgg agatgccgga
     7921 cggcttcgtc accgggacgt tttccctggg ggggcaggtc caggtctccg ggtccgcacg
     7981 ggacgacctg atccgaacgg ccgcggggac gctgaccttc aaggccaagg acggcatgat
     8041 ccggaaattt gccgtcctgt ccaaggtctt ttcgatcctg aacgtttcac aactgttcaa
     8101 gttccaactg cccgacatgg tgagcggcgg catgccttac agccggatca cggcccgcct
     8161 ctccctggcg gacggcgtaa tcgtgaccga ggatctgttc atccggagca acgcgatgaa
     8221 catctcggtg gtgggaaaaa cggatctcgt caagggggtg acggaacaga ccatcggcgt
     8281 gcagccgctc cagacggtgg acaaggtcgt gagccgcatc cccgtcgtcg gctggatcct
     8341 ctcggacgag gacggtcggg tgctgacggt ctatttcgat gcccagggtc cgctcgacga
     8401 tcccgtcgtc caggccgtcc ccgtccagtc cctgtccaaa ggccttctgg acgcgttccg
     8461 gaacctcttc gagctcccgg ccaggctctt caccgacacg ggcgaggtcc tcctcggccg
     8521 ttaagcctcc cggcgcaggc ggttcacgag caggtgcgcg cgccgcgtcg gttccggcag
     8581 acggtaattc cggcagcagg ccaggaccac cgccgccgcc gtctccacgg cgacccgatg
     8641 gcccggcgaa acgaagagcg ggttcgtccg ccgcttcgtc ctcaaaacgg tcccgacgac
     8701 gcgcccgtcc atcctcagat cggatcggct tcccggtgcc tcccccaccg gatcgaacgt
     8761 cccgcacagg cgggttttgg cgcagccgat cgtcgggcgg tcgagccaca aacccaggtg
     8821 ggacgccagg ccgatgcccc ggggatgggc aatgccctgt ccgtcgaaga ggatcaggtc
     8881 cggcggctcg gcgagccggc ccagggccga cagcatgacc ggcccctccc ggaagctgag
     8941 gagcccgggg atgtagggga acgtgacccg ccccacccca tgggcctcct ctttcacgac
     9001 cagttccggg taggtcagga ccacgacggc ggcgaagaaa cggtcgcttc cgcggtcata
     9061 ggagacgtca gcgccggcca cggtccggac gacgccgggc agggcgtccg ccaggaccag
     9121 ccgccgccgc agggcctgct ggatgccgac ggcctcccgg ggatcgcaat cccagcgatg
     9181 ccgtgtttcg atcatgccga tcccccctga tccgccgcgc agcccgaacc ccgcttccgt
     9241 ccggcgcgca cgggcggttt gatggactga catcgggccc ggctcccgcc cggccgattt
     9301 tccatttccc ctctatcacg aagcagaagg cggtccaacg gaattttgcc ttcatccgcg
     9361 gggaccctcg ccgggcagat cccccttgac attttcacgc cttaatacta tccgaaatca
     9421 aaatcacatc gaacggacac ccacaaacga ccgagcacgc cgggaaaccc cggcggacgc
     9481 ggatcggcgt tatcgaatcg ggaggcgtgt tttcaagccc ggcggacgga aaggcggaat
     9541 gcacacgacg gcgagctcga cccccagtcc ggagaatacg ctcggcaacc tgaacagcct
     9601 gatgcgctcg ctgctcgcgt ccatcccgca cgcggtcatc ggcctgaagg accggcgcat
     9661 cgttttcgcc aattccgccg tcaagacggt cttcggctgg cggccggaag aactcatcgg
     9721 ccagacgacc cgcatcctgt atcgctccga ccgcgactat gaagaggtcg cgcgccgctt
     9781 ttaccccatc ctggccaacc ggaaaaccta ccaggcggag tttccctgcc gccgcaagga
     9841 tcagcgcgac atcacctgca tgctcagcgc cgcccgcgtc ggcagccggc tccgggagcg
     9901 gatgatcatc gtgacctacg aggacatcac gcgccgccag gaggaccgcg aggccctgcg
     9961 ggagagcgaa cagttcctca agatgatcct ctcgggatcg ccgatcgcca ccttcgtcat
    10021 cgggcgcgac caccggatca tgttctggaa ccgggccctg gaggaactga cgaagattcc
    10081 cgcccggaag gtcgtcggca cccggcagca gtggcgtgcc ttctaccgga gccgtcggcc
    10141 ctgcatggcc gacctgatcg tcgatcaggc ccacgaaacc atcgcccagt ggtacgccgg
    10201 gacgttcagc aagtcgaacc tcatcgagga cgcctacgag gcgaccgacc atttcccggc
    10261 gctcggggag ggcgggaagt ggctgcgctt caccgcggcg gcgatccgca attccgaggg
    10321 acagctcgtc ggggccatcg aaaccctgga ggacatcacc cagcgccagc aggccgaaga
    10381 ggccctgcgg aaggcgcatg agcacctgga actgcgcgtc caggagcgca ccgccgccct
    10441 ggcccgggtc agcgacgccc tgcaggcgga gctggcggag cgccggcgcg ccgaggaggc
    10501 actgaagcag acgaccgacc agttgtccct gatcctcgaa tcgctcccga tcgtgtcctt
    10561 tacctgccgg ccccgggggc ggtacccgtt cacgttcatc agcagcacga tcgaggagct
    10621 gaccggctat gcgccccggc aattcctgga aaaggccggc ttctggcagg accgcctcca
    10681 cccggacgac cggtcccgcg tgctggccga actgaaggcc ggcatgaaaa gcgggaccct
    10741 gcacacccgc taccgcttcc agatcgcgga tgacggctat cgctggttct ccgacaaccg
    10801 gcgcctgatc acgcttcccg acggcgcctc ccagcacatc gtcggcgcct ggcaggacat
    10861 caccgaggaa acccgcatgc gccaggaggc ggagctgcgc atgcagcaga tgatccagac
    10921 ccacaagctg acggccctgg gcgaggtggt caccggcgta gcgcacgaga tcaacaatcc
    10981 ggtcagcttc atcgcctaca acgccccgct cctcgaagag atctggtcgg tcctggagcc
    11041 gctcctggcg gacctggggc ccgagcggcg cggcgggcaa gtgggaactc tctccttcga
    11101 ggaggtttcc cgcaacatgc gggagatcat cgcggccctc aagatctcct ccaaccgcat
    11161 cagccgcgtg atcgcgagcc tgaaggattt cgcccgcgcg gacgagccgg ccaagacaaa
    11221 gccggtccag atcagcgagg tcatccaagg cgcgctcacg atcgtgggcg gccaggtgcg
    11281 caagaccgtc tcccggatcg aactggacgt cgaggacaat ctgccgttgc tcaggggaca
    11341 cttccagaag atcgaacagg tcatcacgaa cctgctcatc aacgcccacc aggccatgcc
    11401 gcccggccgc aagggcacgg tccggatcac cgcccgccac gtgagatccc tgtccgccct
    11461 ggcgatcgag atcgaggaca acggacgggg catggagcgg gaggtcctga gccatctctt
    11521 cgacccgttc ttcaccaccc ggcgggactg gggggggacg ggcctgggcc tctccatctc
    11581 ctacgggctg atccgggagc acaacggtct gatcggcgtc ctttcccgcc cgggaatggg
    11641 gagccgcttt ctcatcctcc tgccgctgaa cgggcagagc aagattgaac tgcgtccggc
    11701 catgctctgc gtggaccacg accggggctt cgtcagccgg ttgaaggcga atttcgtgga
    11761 cgccgtcgag tatcccttga caagcgatta ccgtccgggg gatattgtcg cccacttgct
    11821 ggagcatccg gagatcgatc tggtgctggc cgaaatcgac ctgcccggcg agggaggctg
    11881 ggccgtactg gatcagatca agcgtcgctt tcccctgctg cccgtcatcc tctgctcccg
    11941 gcggccggcg gccggcgggc gaactccgga agacggcccg cgggccgact tcattctgaa
    12001 gaaaccgttt cgcatcgaac agctccagaa aatgatccgc gaaacgggga ggcagcgtct
    12061 atgagaattc tggtcgtcga cgacgaaacc ctcgccctga cgtccgtcca gcggctgctt
    12121 cgctggcggg gctacccgga tgtggagatc tgcgacagcg gacgcgaggc gatccggaaa
    12181 atccgggaag aggcgttcga catcgtcatt ctcgacctcc tgatgcccga ggtggacggc
    12241 atgcaggtcc tggaggcgac caagccctat cgccccgaga cggagttcat catcctgacc
    12301 gcggtggacg acatccccac gacggtgcgg gccattcgcc tgggcgccta cgattacctg
    12361 gtcaaaccga cggaaaacga actcctgctc ctgtcggtgg aacgggccta cgagcacaag
    12421 gggctggtgg ccggcctgtc ggcctcggtc cggaaggcgc cggcggagat gccggcggcc
    12481 ttcgccgata cgatcacccg ccatccgaag atgatgtccc tgatctccta cgcccaggtc
    12541 atggcgcggg gcgggaaccc catcctgat
//
LOCUS       DAOOLJ010000055        12474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5592_length_12474_cov_16.789436, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000055 DAOOLJ010000000
VERSION     DAOOLJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5592_length_12474_cov_16.789436"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLW16_08240"
     CDS             <1..885
                     /locus_tag="PLW16_08240"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB70934.1"
                     /translation="GDFVYIGGYVCRTPFAAVHEIIRQRKKDLTITRGNAADDFDMMI
                     GAGVVKRFYSTFISLGLYGLARCYRRSLEKGLPRKIEVEEYTNLSLPMMLMAGAMGMP
                     FIPVKDMVGTDLLNIQGFLGDGKYKLIVSPFDGAPVLLAPALHPDVGIIHVQQADENG
                     NAQIWGIGGDCKWGANAAKKVIVSCERIVSREVIGKDPSRTIVPDFKVCAVVEEPFAS
                     HPGYTPGFYDIDFGFGYLYQQASNTVEGFEAFLKEWVYDLPDRAAYLQHYIDRFGYAQ
                     FRRLQAKFGYGYPVSYEY"
     gene            898..1698
                     /locus_tag="PLW16_08245"
     CDS             898..1698
                     /locus_tag="PLW16_08245"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPB70935.1"
                     /translation="MTAHPNDYIKPELMACCGAREIRDGDVVVVGTGFPTMSANIAKH
                     THAPRAVMMQESGVYDARPRRPALSVGDACLNPGAAMIGGLTDVMGMFLQGGWVDVGF
                     LSGSQVDRFGNLNTTCIGPYDRPKNRLPGSGGANPIGALAKRGLIIVQHDPKRLPARV
                     DFITTPGYIDGPGAREKWGLPRGTGPHVIITNKAVLRFDPDTKEAYLASYHPGSTIDE
                     IRAATGWDLRVADDVHETEPPRAEELKTIREVLDPNRMIRIYEGKGYV"
     gene            1715..3082
                     /locus_tag="PLW16_08250"
     CDS             1715..3082
                     /locus_tag="PLW16_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HPB70936.1"
                     /translation="MDHTQNMAAFLAKISLAGLPPEVVHKAKLCLLDYIANVYGSLEL
                     EAVAGAIAYVRSLGGPPAATVLGGGFKTDLHHAAFLNGTTAEAIESQDGLRFGGNHPG
                     TAVIPAALAVAEDRGQGGAAVIEAIVAGYEAANRPAAAMHPWHTLSGFLPTGTCGTFG
                     AATAAARLTGADAAGMLNALGCAGYLAPVSMAEQLMGGYTVKIVQGGQAALAGLMAAG
                     LARAGITGDRQVFEGSDLKGGFTQITTKVEPKFEKLTEGLGEHYTISDIYFKPYTACR
                     HTHGAAQATLELLREQPVDPAEIEAVEVATYGMASVAVGKGVKAGDTIVNAQFSIPYV
                     VAVCLLDGALGPKQLTERRMNDPALLALAQKVTVRMDDELNRVYPERTSSRVEIALTG
                     GGRRVRQVDIPKGDPRDPMEAADLADKVRQFAGDRDRANTERIIAMILDLETLVDIRE
                     LTPLI"
     gene            3200..4366
                     /locus_tag="PLW16_08255"
     CDS             3200..4366
                     /locus_tag="PLW16_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70937.1"
                     /translation="MDFNLNDEQKMLLQTLQALGEREKFKERAAEIDRTGAFPADLMA
                     RYAEMGLLGMTLSADHGGGGQPAINAILAIEELAKFSPMIAAPVFESNVGPVRVIDFF
                     GTEAQKRAIIPGVCRGELSVSVCMTEPEAGSDLTALKTKAVEDGDHYIVNGRKVFITG
                     GGHASHYMVYTRFGDTPGYKGIGGLLIEKGTPGFTFGKQEDFLGLRGMPSCELIFEDV
                     RVLKENCVIQAGDFGKLMLTFDIERCGNAAMCLGTAGGALRESIAYAQARQAFGRPIC
                     EFQAIQFMTVDMAMKLDAARLLVYRAACGAGQGLPSMYEASMGKCFANEMVIDVTNAA
                     MQIFGGYGYSKEFPVERMLRDARAWGVAGGTVQMLRITLASVLYGRRFDQRRGK"
     gene            4482..4922
                     /locus_tag="PLW16_08260"
     CDS             4482..4922
                     /locus_tag="PLW16_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70938.1"
                     /translation="MGTYYRDAQHLYEIYGYFMERVLSDEKIGPKMAKAGIIIRFIYT
                     DPESEITIDLKNPPTKPGLAGSYYLGPCDLKEDVWSKQAADFSHSFWHGLENPVAAVT
                     RGKVKQGGKITAMMKLLPVVKPAFQMFPVILKEMGYENLILKKG"
     gene            complement(4979..5938)
                     /locus_tag="PLW16_08265"
     CDS             complement(4979..5938)
                     /locus_tag="PLW16_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HPB70939.1"
                     /translation="MPREDPLERAEALYKAFLGGTNENYYLEKFHEFDRHLPGIKAGW
                     NWAAFLFGGGWAIYRKMYGWFLFFVEIVLLAGILQGVGAPTIGVAVLSGSWIAFSIYA
                     NALYHASVAKKIADAQRFIINEPMLLQSLRHEGGVNTGFLGGCGLALLMTVWVVVPRF
                     IPQTDRVAPATAPARETGQDGRFVAYSDGTVLDPVTNLMWAAKDNGSDINWPDARSYC
                     RTYRGGGFSDWRMPTQEEIGQLYDSTKRQPAECYRLVFEGSTRYGEIYATPLVDLTCD
                     QLWSSETRGDLACAFAFRIKAAQGWDPQTTAQYYRVLPVRSVR"
     gene            complement(6210..8732)
                     /gene="gyrA"
                     /locus_tag="PLW16_08270"
     CDS             complement(6210..8732)
                     /gene="gyrA"
                     /locus_tag="PLW16_08270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPB70940.1"
                     /translation="MEQLPYQKNVSVDIEDEMRKSYVDYAMSVIIGRAIPDVRDGLKP
                     VHRRVLFAMSEMGNRWNRPYKKSARIVGDIIGKYHPHGDQAAYDTIVRMAQDFSMRYC
                     LIDGQGNFGSVDGDPPAAMRYTEVRMHRLSEEILSDLEKDTVDFVPNYDESLQEPTVL
                     PARIPVLLLNGTAGIAVGVATNIPPHNLREIVDGTVALIQKPDITVDELMKHIHGPDF
                     PTAGFINGREGIVSAYRTGRGIIRMRARALIERNARSDRESIVVTELPYQVNKANLIA
                     AISELVKEKKIGGIADLRDESDREGIRIVIDLKKDEPSAIVLNQLYKHTQMEATFGII
                     MLAIDHGQPRVFNLKEMLESFVEFRRQVVTRRTRFELARAEERAHILKGLLIALDRID
                     EVIAVIKAAANPPAAGEALCARFGLSEVQARAILEMRLQRLTGLEREKIEQEFAELTR
                     LIAELQGILGDPMKILDVIVAELAELKARYGDERRTEIVISSEDISIEDLIVEEDMVV
                     TVSHSGYIKRNPVSLYRSQRRGGRGKMGMQTKEEDFVERVFIASTHNYVLIFTNLGRV
                     YWLKVYQIPQAGRAAKGKALVNLINLNPGERVAAILPVREFVAGKSVVMVTQRGIIKK
                     TDLTAYANPRSTGIIAITIDEGDELIDVQLTTGNQDIFLGTKKGQGIRFHEEDVREVG
                     RTARGVIGIRMDEDDVVVGVEIPAEGTTLLTVSERGYGKRTAIEEYRHQSRGGKGTIN
                     LKTVPKVGTVSGISQVVGDEDILLISDGGKIIRMRVDEIPVIHRSTQGVKLIELEPEE
                     RLVGLARAEREVEEANGQEDALASEDADAPPLDREDDTEPTA"
     gene            complement(8843..11275)
                     /gene="gyrB"
                     /locus_tag="PLW16_08275"
     CDS             complement(8843..11275)
                     /gene="gyrB"
                     /locus_tag="PLW16_08275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPB70941.1"
                     /translation="MENNQVNEKALREHYDAESIKVLEGLEAVRKRPAMYIGSTSKEG
                     LHHLVYEVVDNSIDEAAAGFCDAITVGIRMDNSIVVDDNGRGIPVDMHTTGVSAAQVV
                     LTKLHAGAKFSSDSYKISGGLHGVGVSVVNALSSSLELEVRRDGVLFTQSYERGVPKA
                     PLEKIGKTRGRGTRVTFKPDSEIFDETEFSFDILANRLRELAFLNSGVKITLIDERAD
                     KRGEFFYKGGIVSFVEYINRNKRVLHKHPIFVQGAREDCEVEMALQYNDTYVENIFSF
                     ANSINTTEGGTHLIGFRSALTRVFNNYATTSNLLKNTKESLRGEDLREGLAAVISVKI
                     KNPQFEGQTKTKLGNSEVRGLVEGIVYDKIGVYLEENPAVAKQLLNKCIDAARAREAA
                     RRAKELTRRKSALEVGSLPGKLADCQEKDPAACEIYLVEGDSAGGSAKQGRDRKNQAI
                     LPLRGKVLNVEKARFDKMLQNEEIKVIATALGTGIGKEDFDISRLRYHKVIIMTDADV
                     DGLHIRTLLLTFFFRQMRELVERGFLYIAQPPLFRISDKKTELYIHNEEAMKNHILDM
                     GVGRLRLLTGNGTPLTGSRLSGIVKKVMRIEAILDKFEREDRDREVIRLLAGDPSLAD
                     RDFRAETDLERVARRTALALGDRLIEARVALDPDHGIFKLVFRLRRHDQEQTTCIDRD
                     VLKMPKFLEIKSLLNQVTALGEAPYQVLAEEGENASPVVLPDMAALIEHVMAAGKKGM
                     TLQRYKGLGEMNPGQLWETTMNPEKRTLLQVRVEDAVQADEIFTTLMGDQVEPRREFI
                     FRNALYASNLDV"
     gene            complement(11316..12434)
                     /gene="dnaN"
                     /locus_tag="PLW16_08280"
     CDS             complement(11316..12434)
                     /gene="dnaN"
                     /locus_tag="PLW16_08280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPB70942.1"
                     /translation="MEFTTRRETILEGVQKTLGIVEKKTTMPILNNVLVRAKHGRVKL
                     MATDREIGLITQYDADVSVEGDITLSARKLSEMLRELQGESILFQKQENNQVLISCGK
                     VVYRIPGIPAEDFPLVVFDESVRLYPIKAQVLRDLIRKVYFAMSTDDLRRNLNGVFFE
                     TEKQGEGCQVRMVATDGHRLAKACAGVEDPDFLSLEKGVILPRKGLAEIRKLLEGEDG
                     DVDVAIASGMCMIKTANTVLKVSLIDAEYPDYRRVIPPEPGKQVTFDKNTVLHAFRRM
                     GVMANDRYSGVIVRLTENRMILNSQNPDVGEASDEIEVGYREAEVEVGYNVRYLIDAL
                     EVIDEKDIVFDVGAGMKPGVMRPLGNEQYFCIIMPLKL"
BASE COUNT         2257 a         4026 c         3768 g         2423 t
ORIGIN      
        1 ggcgatttcg tctatatcgg cggctacgtc tgccggacgc cgttcgcggc ggtccacgag
       61 atcatccgcc agcggaagaa ggacctgacg atcacgcgcg gcaacgccgc cgacgacttc
      121 gacatgatga tcggggcggg ggtggtgaag cgtttctact ccaccttcat ttcgctcggc
      181 ctgtacggcc tggcccgctg ctaccggcgc tccctggaaa agggcctccc gcgcaagatc
      241 gaggtggagg aatacacgaa cctgtcgctc ccgatgatgc tcatggccgg ggcgatgggc
      301 atgcccttca tccccgtcaa ggacatggtg gggacggacc tgctcaacat ccagggcttt
      361 ctcggggacg gcaagtacaa gctgatcgtt tctccgttcg acggagcgcc cgtcctcctc
      421 gcccccgccc tccaccccga cgtcgggatc atccacgtcc agcaggcgga cgagaacggc
      481 aacgcccaga tctggggcat cggcggggac tgcaaatggg gcgccaatgc cgcgaaaaag
      541 gtcatcgttt cctgcgagcg gatcgtcagc cgggaggtca tcggcaagga cccgtcccgg
      601 acgatcgtgc cggacttcaa ggtctgcgcc gtcgtggagg agccgttcgc atcgcacccc
      661 ggctacacgc ccggctttta cgacatcgat ttcggtttcg gctacctcta ccagcaggcc
      721 tcgaacacgg tggaggggtt cgaggcgttc ctgaaggagt gggtgtacga cctccccgac
      781 cgggccgcct atctccagca ctacatcgat cggttcggct acgcccagtt ccgccgcctg
      841 caggcgaaat tcggctacgg ctacccggtg agctacgaat attaaaagga gtgtcccatg
      901 accgcccatc ccaacgacta catcaaaccg gaattgatgg cctgctgcgg ggccagggag
      961 atccgcgacg gcgacgtggt cgtcgtcggg acgggattcc ccaccatgtc ggccaacatc
     1021 gccaagcaca cccacgcccc gcgggccgtc atgatgcagg aatccggggt ctatgacgcc
     1081 cgcccccgtc ggccggccct gtcggtcggc gacgcctgcc tgaaccccgg cgccgccatg
     1141 atcgggggac tcacggacgt catgggcatg ttcctccagg gcggctgggt ggacgtgggc
     1201 tttctctccg gctcccaggt ggaccgcttc gggaacctga acacgacctg catcggcccc
     1261 tacgaccggc ccaaaaaccg cctgccgggg agcggcgggg ccaatcccat cggggccttg
     1321 gccaaacgcg gcctcatcat cgtccagcac gacccgaaaa ggctgcccgc ccgggtggac
     1381 ttcatcacga cgccgggcta catcgacggc cccggcgccc gggaaaaatg ggggctgccg
     1441 cgcgggaccg gaccccacgt gatcatcacg aacaaggcgg tgctgcgctt cgatccggac
     1501 acgaaagagg cctatctcgc gagctatcac cccggcagca ccatcgacga aatccgcgcc
     1561 gccacgggct gggacctccg ggtcgccgac gacgtccacg agacggagcc gccccgggcg
     1621 gaggagctga agaccatccg ggaggttctc gacccaaacc ggatgatccg gatctacgaa
     1681 ggcaaggggt atgtataaat aaaggaggaa catcatggac cacacgcaaa acatggcggc
     1741 gtttctcgcg aagatctccc tggccgggct gcccccggag gtcgtccaca aggccaagct
     1801 ctgtctcttg gactacatcg ccaatgttta tggatcgctg gagttggaag ccgtcgcggg
     1861 ggcgatcgcc tacgtccggt ccctgggcgg gccgcccgcg gccacggtcc tgggcggcgg
     1921 cttcaagacg gacctgcacc acgcggcctt cctgaacggg acgacggcgg aggccatcga
     1981 gtcccaggac ggcctgcgct tcgggggcaa ccaccccggg acggccgtga tccccgcggc
     2041 gctcgccgtc gcggaagacc gggggcaggg cggggccgcc gtgatcgaag cgatcgtcgc
     2101 gggctatgaa gcggcgaacc ggccagcggc cgcgatgcac ccctggcaca cgctgagcgg
     2161 ctttctcccg acggggacct gcgggacgtt cggggcggcc accgccgccg cgcgtctgac
     2221 cggggccgac gccgcgggca tgctcaacgc cctcggctgc gccgggtacc tggcccccgt
     2281 ctccatggcg gaacagttga tggggggata caccgtcaag atcgttcagg gcggccaggc
     2341 ggccctcgcc ggactgatgg cggccgggct tgcccgggcc gggatcacgg gggaccgaca
     2401 ggtgttcgag ggatcggacc tgaagggcgg cttcacgcag atcaccacca aggtcgagcc
     2461 gaaattcgaa aagctcacgg aaggactggg cgagcattac acgatctccg acatctactt
     2521 caaaccctac acggcctgcc gccacacgca cggcgccgcg caggcgaccc tggaactcct
     2581 gcgggaacag ccggtggacc ccgctgaaat cgaggcggtc gaggtggcca cctacgggat
     2641 ggcgagcgtc gccgtcggca agggggtcaa ggccggcgac accatcgtga acgcccagtt
     2701 ctccatcccc tacgtcgtcg ccgtctgcct cctcgacggc gcgctcgggc cgaagcagct
     2761 cacggaacgg cgcatgaacg atcccgccct cctggctctg gcgcagaagg tcacggtcag
     2821 gatggacgac gaactgaacc gggtctaccc tgagcggacg tcgagtcgcg tggagatcgc
     2881 cctcacgggc ggcgggcggc gcgtccgcca ggtggacatc ccgaagggcg atccgcggga
     2941 tccgatggag gccgccgatc tggccgacaa ggtccggcag ttcgccggcg accgggaccg
     3001 ggcgaatacg gagcggatca tcgcgatgat cctcgacctc gaaaccctcg tggacatccg
     3061 ggaattgacc cccctgattt gaacatcgac cgtggcgcgg ttgtggatcg ggggtcgttt
     3121 gagttccacg tcatacatcg gtatacggaa aaagaatccc ttgcaaaggg cgtgatgatc
     3181 gaaaaaaggg gcgtaaaata tggatttcaa tttgaacgac gaacagaaga tgctgctcca
     3241 gaccctccag gccctggggg agcgcgagaa gttcaaggag cgggcggcgg agatcgaccg
     3301 gacgggggcg tttcccgccg acctgatggc ccgctacgcc gagatgggcc tcctgggcat
     3361 gacgctgtcg gccgaccacg gcggcggcgg ccagccggcg atcaacgcca tcctcgccat
     3421 cgaggagctg gccaagttca gccccatgat cgccgccccc gtcttcgaat cgaacgtggg
     3481 gccggtccgg gtgatcgact tcttcgggac ggaagcgcaa aaaagggcca ttatcccggg
     3541 ggtttgccgt ggcgaactca gcgtgtccgt ctgcatgacg gagccggaag cgggttccga
     3601 cctgaccgcg ctgaagacga aggccgtcga ggacggcgac cattacatcg tcaacgggcg
     3661 gaaagtcttc atcaccggcg gcggacacgc gagccactac atggtctaca cccgcttcgg
     3721 cgacaccccc ggctacaagg ggatcggcgg tctgctcatc gagaagggaa cgcccggctt
     3781 caccttcggc aagcaggagg atttcctggg gctcaggggc atgccctcct gcgagctcat
     3841 cttcgaggac gtccgggtcc tcaaggaaaa ctgcgttatt caggccgggg acttcggcaa
     3901 gctcatgctg accttcgaca tcgagcggtg cggcaacgcc gccatgtgcc tcgggacggc
     3961 ggggggggcg ctccgggaat caatcgccta cgcccaggca cgccaggcgt ttggccggcc
     4021 gatctgcgag ttccaggcga tccagttcat gaccgtggac atggcgatga agctcgacgc
     4081 ggcgcgcctg ctggtgtacc gggccgcctg cggcgccggg cagggcctgc cctccatgta
     4141 cgaggcctcg atggggaagt gcttcgccaa cgagatggtc atcgacgtga cgaacgccgc
     4201 catgcagatc ttcggcggct acgggtacag caaggaattt ccggtggagc ggatgctccg
     4261 ggacgcccgg gcctgggggg tggccggggg gaccgtgcag atgctccgga tcaccctagc
     4321 gagcgtcctg tacggccggc gcttcgacca gcgccgggga aagtaggggg cggtccggga
     4381 gttttcccgc aggccaatcg ggacaagatc ctgagcgggc gggaagactc acggacccgt
     4441 ttgagattgc aacgtgacag catcatcatg aggaggggga aatgggtacg tattaccgcg
     4501 atgcgcagca tctttacgaa atctacgggt atttcatgga gcgggtcctc tcggacgaaa
     4561 agatcggccc aaagatggcc aaggcgggta tcatcatccg cttcatctac accgacccgg
     4621 agtccgaaat caccatcgat ttgaagaacc ccccgaccaa accgggattg gccggaagtt
     4681 actacctcgg cccctgtgac ctgaaggagg acgtctggtc gaagcaggcg gcggactttt
     4741 cccacagctt ttggcacggc ctggagaacc ccgtcgccgc cgtgacccgg gggaaggtca
     4801 aacagggagg caagatcacc gcgatgatga agctcctgcc cgtggtgaag cccgccttcc
     4861 agatgttccc cgtcatcctg aaggagatgg gttatgagaa cctgatcctc aaaaagggtt
     4921 aggaaaaccg ccccatgccc cgccattccc catcgacccg gattgctcct tgatcctctt
     4981 atctaacgga gcggaccggc aggacgcgat agtattgggc ggttgtctgg ggatcccatc
     5041 cctgggcggc cttgatgcgg aaggcaaagg cgcaggcaag gtcgccccgc gtttccgatg
     5101 accacagttg gtcgcacgtc aggtcgacca agggggtggc atagatctcc ccgtaacggg
     5161 tgctcccctc aaacacgaga cggtaacatt ccgccggctg ccgtttggtc gagtcataca
     5221 attgcccgat ttcctcctgc gtcggcatcc gccagtccga aaaccctccc ccgcggtagg
     5281 tccgacaata gctccgggca tccggccagt tgatatcgga tccgttgtcc tttgccgccc
     5341 acatcaggtt cgtgaccggg tccaggacgg tcccgtcgct gtacgccacg aaccggccat
     5401 cctggcccgt ttcccgtgcg ggggccgttg cgggagccac cctgtccgtc tggggaatga
     5461 accgcggaac cacgacccag accgtcatga gcagggccaa gccgcacccc ccgagaaacc
     5521 ccgtgttcac gccgccctca tgccggaggg actgaagcag catgggttca ttgatgatga
     5581 agcgctgcgc gtcggcgatt ttcttcgcga cactggcatg atacaaggcg tttgcgtaaa
     5641 tcgaaaaggc gatccacgag ccggataaca cggcgacgcc gatggtcggg gccccaacac
     5701 cctgcaaaat ccccgcgagc aaaacgattt ccacgaaaaa aaggaaccat ccatacattt
     5761 tccgatagat ggcccagccc ccgccgaata aaaacgcggc ccagttccag cctgccttta
     5821 tccccggaag gtgtcggtcg aattcatgaa atttctcgag gtagtagttc tcgtttgttc
     5881 ccccgaggaa ggctttgtag agtgcctcag cccgctccag cgggtcttcc cggggcacgg
     5941 agtccgccga aaccggctga ggcggcgaca ccgggggcgc ttgtttttga aaatcttccc
     6001 ggccctccgg tcgggattca ggggcttccc gcccgtccag accctcccgg gggggtgtct
     6061 ggacccgccg ttcaccgcaa tagacgcatt tcgcggcgcc tctttgtatc cactcctcat
     6121 cgcagaatgg acattttgcc ataaccggcc cccacggaaa tccccgaacc ccgccattcc
     6181 ccgggatgcg tcatcccgcg aagaaacccc taagccgttg gttccgtatc gtcctcccga
     6241 tccaggggag gcgcgtccgc gtcttctgaa gctagggcgt cctcctgacc gttcgcttcc
     6301 tccacctccc gctccgccct cgccagtccc accaggcgct cctccggctc gagttcgatc
     6361 agcttgaccc cctgtgtgct gcgatggatc acggggattt cgtccacgcg catccggatg
     6421 atcttccctc cgtcgctgat gagcaggatg tcttcgtcgc cgacaacctg ggagatgccg
     6481 gagaccgtgc cgaccttcgg gacggtcttc agattgatcg tccccttgcc gccccggctc
     6541 tggtgccggt actcctcgat ggccgtccgt ttgccgtagc cccgctcgga gaccgtcaag
     6601 agcgtcgtcc cctcggcggg gatttccact ccgacgacga cgtcgtcctc gtccatccgg
     6661 atcccgatga cgccgcgcgc ggtccgcccc acctcccgga cgtcctcctc gtggaagcgg
     6721 atgccctgcc ccttcttggt ccccaggaag atatcctgat tgcccgtggt gagctgcacg
     6781 tcgatgagtt catcgccctc gtcgatcgtg atggcgatga tccccgtcga acgcggattg
     6841 gcataggccg tcagatccgt cttcttgatg atcccgcgct gggtcaccat gacgacggac
     6901 tttccggcga cgaattcccg gaccggcagg atggccgcca cccgttcacc ggggttcagg
     6961 ttgatcaggt tgacgagagc cttgcccttc gccgcccgtc cggcctgtgg aatctggtag
     7021 accttgagcc agtacacccg gcccaggttc gtgaagatca gcacgtagtt gtgcgtggag
     7081 gcgatgaaca cccgctctac gaaatcctct tccttcgtct gcatccccat cttgccgcgt
     7141 ccgccgcggc gctgactgcg gtagaggctc acgggatttc gcttgatgta gccgctgtgg
     7201 gagacggtca cgaccatgtc ctcttcgacg atcaggtcct cgatgctaat gtcctcggag
     7261 ctgatcacga tctccgtgcg gcgttcgtcc ccgtagcggg ctttgagctc cgcgagctcc
     7321 gcgacgatga cgtccaggat cttcatcggg tcgccgagga tcccctgaag ttcggcgatg
     7381 agccgggtca gttcggcgaa ttcctgctcg atcttctccc gctccagccc cgtcaggcgc
     7441 tggaggcgca tctccagaat ggcccgggcc tgaacctcgg acagaccgaa gcgggcgcac
     7501 aacgcttcac cggccgctgg agggttcgcg gccgccttga tcacggcgat gacctcgtcg
     7561 atccgatcaa gcgcgatcag cagtcccttc aggatatggg cgcgctcctc ggcccgcgcc
     7621 agctcgaatc gggtccggcg cgtcacgacc tgccggcgaa actccacgaa gctctccagc
     7681 atttccttca ggttgaagac gcggggctgg ccgtggtcaa tggccagcat gatgatgccg
     7741 aaggtcgcct ccatctgggt gtgcttgtag agctggttca gcacgatggc cgacggctcg
     7801 tccttcttga ggtcgatgac gatccggatg ccttcacgat ccgactcgtc ccggaggtcg
     7861 gcgatgcccc cgattttctt ctccttgaca agctcggaga tggcggcaat caggttggcc
     7921 ttgttcacct ggtagggcag ttccgtgacg acgatgctct cccggtcgct acgggcgttg
     7981 cgctcgatca gggcgcgggc gcgcatccgg atgatgcccc ggccggtccg gtaggcggag
     8041 acgatcccct cccggccgtt gatgaatccg gccgtcggaa aatcagggcc gtggatatgc
     8101 ttcatgagtt cgtcgaccgt gatgtccggc ttctggatca gggcgacggt cccgtcgacg
     8161 atttcccgca ggttgtgcgg ggggatgttc gtcgcgacgc ccacggcgat gcccgccgtc
     8221 ccgttcagca gcagaacggg aattcgcgcc ggcagaaccg tcggctcctg aagggactcg
     8281 tcgtagttgg ggacgaaatc caccgtatcc ttctccaggt cggacaggat ttcctccgag
     8341 agccgatgca tgcggacttc cgtgtaccgc attgcggccg gggggtcccc atcgacggag
     8401 ccgaagttgc cctggccgtc gatcaggcag tagcgcatgg agaaatcctg ggccatgcgc
     8461 acgatcgtgt cgtaggccgc ctggtcccca tggggatggt acttgccgat gatgtccccg
     8521 acgatgcggg ccgatttttt gtagggccgg ttccagcggt tgcccatttc gctcatggcg
     8581 aacagcaccc ggcggtgcac cggcttcagg ccgtcgcgga cgtccggaat ggcgcgcccg
     8641 atgatgacgc tcatggcgta gtccacgtag gactttctca tttcgtcttc tatgtctacc
     8701 gagacattct tctgataagg cagttgctcc atgtgtaacc tttcaatcca tatcggcgtt
     8761 gggcgtcacg tgcaaaacgt cgggagggag gtctcgggtc gatcatcccc gctccgacga
     8821 ctcctgacgg gcgcctgctt ctttacacat ccagatttga tgcgtacagg gcgtttcgga
     8881 aaatgaactc ccggcgcggt tcaacctggt cgcccatgag tgttgtgaag atctcgtccg
     8941 cctgaacggc gtcctcgacc cggacctgca gaagcgtccg cttttccggg ttcatcgtcg
     9001 tctcccagag ctgccccggg ttcatttcac cgagaccctt gtaacgctgc agggtcatgc
     9061 cctttttgcc ggcagccatg acgtgctcga tcagggcggc catgtcgggc aggacgacgg
     9121 gcgacgcgtt ttcgccctct tccgccagaa cctggtacgg ggcctcgccc aaggccgtca
     9181 cctggttcaa cagggatttg atttccagga atttcggcat cttcaggaca tcccggtcga
     9241 tgcaggtcgt ctgttcctgg tcgtgtcgcc ggaggcgaaa gaccagcttg aagatgccgt
     9301 gatccgggtc gagggcgact cgggcctcga tcagccggtc tcccagggcc agggccgtgc
     9361 gccgtgccac gcgctcgagg tccgtctcag cccgaaaatc gcggtccgcg agtgatgggt
     9421 cgccggccag gaggcggatg acctcccggt cccgatcctc gcgttcgaac ttgtccagga
     9481 ttgcctcgat ccgcatgacc ttcttgacga ttccggacag gcggctcccc gtcagcggcg
     9541 taccgttgcc ggtcagcaag cggaggcggc cgacgcccat gtccagaata tggtttttca
     9601 tggcctcttc gttgtggatg tacagttcgg tcttcttgtc gctgatgcgg aacaggggcg
     9661 gttgggcgat gtacaggaac ccccgctcga cgagttcgcg catctgacgg aaaaagaagg
     9721 tcaaaagaag cgtccggatg tggaggccgt ccacgtcggc gtcggtcatg atgatgacct
     9781 tgtggtagcg caaccgtgag atgtcgaagt cttccttccc gatccccgtt cccaaggccg
     9841 tcgcgatgac cttgatttcc tcgttctgca gcattttgtc gaagcgggcc ttctccacgt
     9901 tcaggacctt gccccgcaag gggaggattg cctggttttt ccggtcccgg ccctgcttgg
     9961 ccgatccgcc ggccgagtcc ccctccacca aatagatctc gcaggccgcc gggtccttct
    10021 cctggcaatc cgcgagcttc cccggcaggg acccgacttc cagggcgctc ttgcggcggg
    10081 tcagctcctt ggcgcggcgg gcagcctcgc gcgcccgcgc cgcatcgatg cacttgttca
    10141 ggagctgttt ggccacggcg gggttctctt ccagatagac cccgatcttg tcgtagacaa
    10201 tgccctccac cagtccccga acctcgctgt ttcccaattt ggtcttcgtc tggccctcga
    10261 actgcgggtt tttgatcttg acgctgatga cggcggccag tccttcccgc aggtcctccc
    10321 cgcgcagcga ctctttcgtg tttttgagga gattgctcgt ggtcgcatag ttgttgaaca
    10381 cccgcgtcaa ggcggagcgg aaaccgatca ggtgggttcc gccctccgtc gtgttgatgc
    10441 tattggcaaa ggaaaagatg ttttccacat aggtatcgtt gtattggagg gccatctcca
    10501 cctcgcagtc ctcccgtgcg ccctgaacga agatggggtg cttgtggaga acccgcttgt
    10561 tgcgattgat atactccacg aaggagacga tgcccccttt gtagaagaat tctcctcgct
    10621 tgtcggcccg ttcgtcgatc agggtgattt tcacgccaga attgagaaag gccagttctc
    10681 ggagacgatt ggccagaatg tcgaagctga actccgtttc gtcgaagatc tccgaatccg
    10741 gcttgaaggt gacccgcgtt ccccgtcccc gggtcttgcc gatcttttcc aagggcgcct
    10801 tgggaacgcc gcgctcgtag gattgcgtaa atagaacccc gtcccgtctc acctccaact
    10861 ccaagctgct cgacagcgca ttcaccaccg aaacacccac gccgtgcagt ccaccggaga
    10921 tcttgtagct gtcgctggag aacttcgccc cggcgtgaag cttggtcaac acgacctgcg
    10981 ccgccgaaac gccggttgtg tgcatgtcca cggggatgcc acgcccgtta tcgtccacaa
    11041 cgatgctgtt atccatgcgg atcccgacgg tgatggcatc gcagaatccg gcggctgcct
    11101 cgtcgatgct attgtccacc acctcataga ccaagtgatg aaggccttcc ttgctcgtcg
    11161 acccgatgta catcgccggc cgctttcgga cggcctccag accctccagc actttgatgc
    11221 tttccgcgtc gtaatgctcc ctcagcgctt tttcgtttac ctgattattt tccatagtta
    11281 accttcattg cctcgttgac cggatccctt gacgtttaaa gcttcaaggg catgataata
    11341 caaaagtact gttcatttcc caagggacgc atcactccgg gtttcatccc cgcaccgaca
    11401 tcaaatacga tgtccttctc atctatcact tccaaggcat cgatcaggta gcggacgtta
    11461 taccccacct ccacctccgc ctcccgatag cccacctcga tctcgtcgct ggcctcaccg
    11521 acatccggat tttgagaatt caggatcatc cgattctccg tcaggcggac gatgaccccg
    11581 ctgtaccggt cgttggccat gacccccatg cgacggaatg cgtgcaaaac cgtattttta
    11641 tcgaacgtga cctgctttcc cggctcgggg gggatgaccc gccgataatc ggggtattcc
    11701 gcatcgatca ggctgacctt caaaaccgta ttggccgtct tgatcatgca catcccggac
    11761 gcgatggcga cgtcgacatc gccgtcctcc ccctccagca atttccggat ttcggcgagg
    11821 ccctttcgcg gcaggatgac ccctttttcc agggacagaa aatcaggatc ctctacgccc
    11881 gcacaggcct tcgccagacg atgaccgtcc gtggccacca tcctcacctg acagccctcc
    11941 ccctgctttt ccgtttcgaa aaagacgcca ttgaggttcc ggcgcaggtc gtccgtcgac
    12001 atggcaaaat agaccttccg gatcagatct ctcagcacct gcgccttgat gggatacagt
    12061 cgcaccgact catcgaagac gaccagggga aaatcctcgg cgggaatccc gggaatccgg
    12121 tacaccacct ttccgcaact gatcaggacc tgattgtttt cctgtttttg aaacaggatg
    12181 gactcccctt gaagttccct gagcatttcc gataatttgc gtgcagaaag cgtaatgtcg
    12241 ccctcaacag acacgtctgc gtcgtactgc gtgatcagac cgatttcccg gtccgtggcc
    12301 atcagtttga cccggccatg cttcgccctg accaggacgt tgttcaagat gggcatggtg
    12361 gtcttctttt ccacaattcc cagggtcttt tgcacccctt ccaggattgt ctcgcgccgg
    12421 gtcgtgaatt ccatatttcc catcctttct tttagactga agttaaatta atta
//
LOCUS       DAOOLJ010000056        12293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5703_length_12293_cov_18.961677, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000056 DAOOLJ010000000
VERSION     DAOOLJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5703_length_12293_cov_18.961677"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..609)
                     /locus_tag="PLW16_08285"
     CDS             complement(37..609)
                     /locus_tag="PLW16_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70943.1"
                     /translation="MEKTERDSYGQPMVDFHWEAPSLEGEGTITFEDGSKYKGSFKAG
                     RLDGYGTFTWPDGSRYEGQWREDLPHDLGTLQRADKRMYSGEWKQGIAEGEGAETLPD
                     GARYSGQWKNGLRNGYGEMNFAEGKKYNGEWRDDMQHGTGELFLTDGSKYEGTWVENK
                     MSGAGVLVFWDGKRYRGVWENEKFNGHFEV"
     gene            complement(596..979)
                     /locus_tag="PLW16_08290"
     CDS             complement(596..979)
                     /locus_tag="PLW16_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70944.1"
                     /translation="MAYLAKLQQDSKTLSKDKPRQPLHQKITLPSPDPQPTGARPLNQ
                     PQRGSSPAEVIMDYPDGRKYVGGWSAGMPEGDGIMTSPDGSKYMGQWRQGKKNGVGIL
                     ITADGKRIPGTWKNDEYVGKPHGKD"
     gene            1058..2332
                     /locus_tag="PLW16_08295"
     CDS             1058..2332
                     /locus_tag="PLW16_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70945.1"
                     /translation="MRRLFRPGLGFRRGLPARFFVGPYSGLSFRFAPFEFLLRAKPGI
                     LADFLLHGFVGLAAGFFFGLTSGFFFRLAAGFFFGLTSGFFFRLAAGFFFGLAAGFLF
                     GLTAGFLFGLTPGLFFGLAAGFLFGLTAGLFFGLLSGLRLLAPGCRLGFLPREFLRLL
                     PDFLLDPPLLLVLVAQVGLLIDRPGRRNRRYRFGLTACLLLGVQSGFLLSLALCLRLG
                     PLPGFLFRLQTGLFFGLAAGFRLDFLPGLSFCFPLGFLFRLRLRLPRSVLTFVFPGFS
                     RFPFPLNFAAVGPGENTPPVRQIVFPLSLVTAPVRPGHGAETPQMSLQNLAFITAAVG
                     PGDFALRPVDVRDPGQSEAVFLCRVDNQHKKPEASMSGIYDPLKRNPVLAAVNRKKTP
                     VHPEISRNIKRLTSFSGRGPTGKNNPLLYQIV"
     gene            complement(2465..2929)
                     /locus_tag="PLW16_08300"
     CDS             complement(2465..2929)
                     /locus_tag="PLW16_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70946.1"
                     /translation="MNLQVVLALLAVAVALVVAVWRFSGGSYGKLRPSAEASAAYEAF
                     QVQPDLTYYISGSDVHPNALMGIDASWTLASDLWKKRDLTPEGLKELVRGMQAKALER
                     LAPLQGFDILDHRDRQIGTWFSLPGPHVSIRIREENRVEISTPPLNTYHMDE"
     gene            complement(2994..3740)
                     /gene="gpmA"
                     /locus_tag="PLW16_08305"
     CDS             complement(2994..3740)
                     /gene="gpmA"
                     /locus_tag="PLW16_08305"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPB70947.1"
                     /translation="MPTLILLRHGESLWNRENRFTGWTDVDLSETGKNEALEAGRLLK
                     REGYLFDLAFTSVLKRAIRTLNIVLEEMDLEWIPVVKSWRLNERHYGALQGLNKAETA
                     VRYGEAQVKLWRRSYEVPPPDLTPEDARYPGHELKYRDLTAAELPLTESLKDTAARVM
                     PFWRDEIAPAIRQGKRVLIAAHGNSMRALVMYLDRISETEIVGLNIPTGIPLVYELDE
                     ALQPLRHAYLGDPDKVQAAARAVADQGKMR"
     gene            4216..5697
                     /locus_tag="PLW16_08310"
     CDS             4216..5697
                     /locus_tag="PLW16_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70948.1"
                     /translation="MTNARKAEQGPCRATFPHGGGERRIVVIGAGAAGMMAAGRAAER
                     GAEVLLCEKTEHPGKKLLVSGKTRCNLTNARELDGFMGMYGPGGPFLHAAFRRFFRED
                     LLAFLERYGVETKTERGGRIFPVSDRAEDVLAAFRAYLKEQGVRLRTGSPVTAIRTEA
                     ARVTGVQTSRDLYPAAAVILATGGATWPATGSTGDGYRLAAALGHRIVPLRPALVPLA
                     VREISLARGLQGVSLRNVRLTAFRGPADGIDPGRIPENECGRGIGGRKPRPPVIESRM
                     GEMMFTHFGIGGPIVLLMSLAVVDALVAGPVGVSIDLKPALTPARLRERLQRDFDRHG
                     RRQFRRHLEGLLPAKMVAPFLALTDIPADKPGHQIAASERDRLAGLLKSLRFNVKAPL
                     PMASAIVTAGGIALEEVDPRTMASRKVSGLFFCGEVLDIDADTGGYNLQAAFSTGYLA
                     GEGAADVVLSPDVSAREGHGRERTGIEVVGQEPETFLRREGAS"
     gene            5694..8123
                     /locus_tag="PLW16_08315"
     CDS             5694..8123
                     /locus_tag="PLW16_08315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPB70949.1"
                     /translation="MKWLKAGVVLAVILILAVVAGGMICLRINPVQREGEIRLSGLQA
                     PVRVVRDGNGTPYIYARTLDDALFAQGFVTAQDRLFQMQLTRMFAAGRIAEMVGEKAR
                     NLDIRMRTIGFVRQARRHEAILDARTRRFLGRYLEGVNAFIHQGRDLPVEFKLAGIRP
                     EPWEIADSLSVIYLMGWDSAANLKSEIVARMLVERLGPEKAREIFPLNINPDERLGKT
                     GAVFRKPSPQEVRRQIILSGDAGLIAFLKDVRPLAAGSNNWAVGPKRSASGKPIVAND
                     PHLDARILPGPWYPSGLFTPEFRAVGVTIPGVPGIVAGRNDRIAIGMTNAYGDAQDLF
                     IETLDPKDPGRYLEGRVSRPFQVVPETIRIRDKKARGGFREETVQVRLTRRGPVISGL
                     FPGLEAEKAEKAEKAVTVRWSPFETMGPSIGLERLFTARSVGQVRQALGNVTTIMLNF
                     VFADVDGHIGWQTTGRLPIRARGDGLLPVEVRDGSDPWRGWIPYAQMPARYDPPRGWL
                     GTCNHMTVTRDYPYAYSTHLSPSFRYRRLAELLDAPGKVSAHKNWLYQRDMMNLLARD
                     VAPVMARVLLGAKETEKMGRILNKWDYYDYAGRSGPAIFHAVWIKFARRVFQDELGPE
                     LAGLMLDNWYFWEERLRAMVLAGESPWFDDVSTPRRETMADLFREAGREAAHELKDTL
                     GDNPARWQWGKLHRIEFVSPLRREGFGRGLVGGGSHPMGGSPETLYRGIYDFNKPYAV
                     NVSASLRMVADLGDPDRVYAVLPGGVSGRLFDRHNRDQIRPFMDGEKRAWWFSDAMIE
                     KHAKYRLELKP"
     gene            8141..8809
                     /locus_tag="PLW16_08320"
     CDS             8141..8809
                     /locus_tag="PLW16_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPB70950.1"
                     /translation="MELFETMETRSSRRAFLSRPVERDVLERLIAAANRSPSYQNTQP
                     WEVFVATGEKKDAVAARMHDNVVKGTPMEPHIPFARVLPKAHETRALSHRNRRFQALG
                     IDPEKDQERITESYMRNFMFFGAPCVIFVGMDKTLSPWSIFDLGAFVHAFLLAAHAAG
                     LGAVPQAMPTGYPAIVREELGISEDIAIILALSAGYPDLDAPVNGYRSLRRETVEFVR
                     WIGF"
     gene            complement(8806..10170)
                     /locus_tag="PLW16_08325"
     CDS             complement(8806..10170)
                     /locus_tag="PLW16_08325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB70951.1"
                     /translation="MEERFDLIVIGGGPGGHAAAETAARLGARVCAIERDGWGGTCTH
                     RGCIPTKALLACSGHYAALKRLGRLGIGIGEASFDFAAMKRHQGQLVRISALGVRKSL
                     EAAGAVLKEGEGRLRAPGEVDCVTPDGRRETLHAKNVCLAWGSSPLLPAGFVPSARIL
                     TSDGLLALPNLPERIAIVGGSVIGVEFATLLAELGCRVTLWELLDRLIPTEDPDLGGF
                     LAQELKRLGVEIRTGVRMTAIEEDEDGVALTAEPAERDGETPALQWRGTCVLLAAGRR
                     PSLDEDALKRLGIDYGLQGIRVDTRQQTTVPGIQAVGDVTGGMMLAHRAMQQGRALAD
                     RLFGAGTISCDERAAPAVIYTHPPIARVGLTEQEAAARGIEIEIVRPDYAANIMARAE
                     LVPTGFAKLLFHRGTFAGAAVAGEAAGDLIAPLALAVSANLGRRELANWILPHPSLSE
                     LLNV"
     gene            complement(10242..10745)
                     /locus_tag="PLW16_08330"
     CDS             complement(10242..10745)
                     /locus_tag="PLW16_08330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB70952.1"
                     /translation="MAEKQWDRPPEMQIDPGKRYHIRMETGRGAIELELYPEHAPKTV
                     NNFVFLAREGFYDGVTFHRVIADFVIQGGDPTGTGRGGPGYRFEDECRGNPLRHDSQV
                     ISMANAGPDTNGSQFFITHSPQPHLDGKHTVFGKVVSGQAVVNAIRQGDCMERVTVSE
                     TAPGAPG"
     gene            11221..>12293
                     /locus_tag="PLW16_08335"
     CDS             11221..>12293
                     /locus_tag="PLW16_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB70953.1"
                     /translation="MKQGKGIRGAMQQLLAVVLLLAAATPASAQWLEEAVREHTLANG
                     MKLLMVERHASPTVSAWIRYRVGSADERSDERGVAHLLEHMLFKGTKTLGTRDYAAER
                     PLLERIEATARALVAEKARRAKGDRERIARLEEELAALEKEAARYTVREEFFEIYARN
                     GGTGYNAFTGKDGTTYLVSLPANKLELWAAIESDRMMNPVLREFYSERAVVMEERRRS
                     YDTQPEGRLWENFLATAFVAHPYGQPIIGWMSDLENLTRTRAEAFLHRYYAPNNAVVA
                     VVGDIDPDRTIALVERYFGAIPPGVDVPPVAVDEPPQAGEKRIELRLDSEPHLLVGFH
                     KPVYPNPDDDVFDVIEMILAEGKT"
BASE COUNT         1895 a         3985 c         4071 g         2342 t
ORIGIN      
        1 ttttcggtgg acgtgatttc actcatgccg cgcgtttcac acttcaaagt gaccgttgaa
       61 tttctcgttt tcccagacgc cccggtatcg tttgccgtcc cagaagacca ggacgccggc
      121 gcccgacatc ttgttttcca cccaggtccc ttcgtacttg gaaccgtcgg tcaggaaaag
      181 ctcccccgta ccgtgttgca tgtcgtcccg ccactcgccg ttatactttt tcccctcggc
      241 gaagttcatc tctccatagc cgttccgcag gccgtttttc cactggcccg agtagcgagc
      301 gccgtcgggc agcgtctccg cgccttcgcc ctccgcgatg ccctgtttcc attcgccgga
      361 gtacatgcgc ttgtccgcac gctggagcgt ccccaggtca tgggggagat cctcgcgcca
      421 ctggccttcg tagcggcttc catccggcca ggtgaaggtc ccgtaaccgt caaggcggcc
      481 ggccttgaag gatcccttgt attttgagcc gtcctcgaag gtgatcgttc cttcgccttc
      541 cagggaaggc gcctcccagt gaaaatcgac catcggctgt ccgtatgaat cccgctcagt
      601 cttttccatg aggctttccc acgtactcat cgtttttcca ggtgccggga atccgcttcc
      661 cgtcggccgt gatcaggatt cccaccccgt ttttcttgcc ctgccgccac tggcccatgt
      721 acttcgaccc gtcgggcgaa gtcatgatcc cgtccccctc gggcattccg gcgctccaac
      781 ctccgacgta tttcctgccg tcgggatagt ccatgatgac ctcagccgga ctcgatccgc
      841 gctggggttg attgaggggg cgggccccgg tgggctgggg gtccggggaa ggcaacgtaa
      901 tcttctgatg gaggggctgc cgtggtttgt ccttcgagag ggtctttgaa tcctgctgaa
      961 gtttggccag atacgccatc tgcagcagtc ctccctcgtc cctgcggcgg aaaggtcttc
     1021 cggcatcctc gacgggaggc gtttccgcct tcattttttg cgccggctct tccgcccggg
     1081 gcttggtttc cgccgcggtc ttcctgcgcg tttcttcgtc ggcccgtatt ctggcctgag
     1141 cttccgcttt gcgcctttcg aattcctctt gcgcgcgaag ccgggcatcc tcgccgattt
     1201 tcttctgcat ggattcgtcg gtcttgcggc gggcttcttc ttcggcctta cgtcgggctt
     1261 cttcttccgt cttgcggcgg gcttcttctt cggccttacg tcgggcttct tcttccgtct
     1321 tgcggcgggc ttcttcttcg gccttgcggc gggcttcctc ttcggcctta cggcgggttt
     1381 cctcttcggc cttacgccgg gcctcttctt cggccttgcg gcgggcttcc tcttcggcct
     1441 tacggcgggc ctcttcttcg gccttctttc tggcctccgc ctccttgcgc cgggctgccg
     1501 cctcggcttt cttccgcgcg agttcctccg tcttttgccg gatttcctcc tcgatccgcc
     1561 tctgctcctc gtcctcgtcg cccaggtcgg cctcctcatc gaccgtcccg gtcgccggaa
     1621 ccgccgctac cgtttcggcc ttacggcgtg tctcctcctc ggcgtgcagt cgggcttcct
     1681 tctctctctg gcgctgtgcc tccgcctcgg cccgttgccg ggcttcctct tccgccttca
     1741 aaccggcctc ttcttcggcc ttgcggcggg cttccgcctc gactttttgc cgggcctctc
     1801 cttctgcttt ccgttgggtt tcctcttccg ccttcggttg cgactccctc gctcggtgct
     1861 gacgttcgtc ttccctggtt tttcccgctt cccattcccc ctgaactttg cggccgtcgg
     1921 gccaggtgaa aacaccccgc ccgtgcggca gatcgttttc ccactctccc ttgtaactgc
     1981 tcccgtccgg ccaggtcatg gcgccgagac cccgcagatg tccttgcaaa atctcgcctt
     2041 cataacggcg gccgtcgggc caggtgattt tgcccttcgc ccggtcgacg ttcgcgaccc
     2101 gggtcaatcg gaagcggttt tcctctgcag ggtcgataat cagcataaaa aaccggaagc
     2161 ctctatgtcc ggcatatatg atccattaaa gcgaaaccct gtcctcgccg ccgtgaaccg
     2221 taaaaaaaca cctgtgcatc ccgaaatcag tcgtaatata aaaaggttga cttcattttc
     2281 gggccgcggc cctacgggca aaaacaatcc tctcctctac cagatcgttt gaattgtaat
     2341 ggaaatcatc ggttatgtca acgggaaagg cgaacccacg gggacgggga aggggtgcgc
     2401 gcaagggccg ttttcgatgc ttccatgtcc cggaatctcc gggcggaatg ccatcgggtg
     2461 ggcctcattc atccatgtgg taggtattca gggggggcgt ggagatctcc acccggtttt
     2521 cctcccggat ccggatggac acgtgcgggc cgggcaggga gaaccacgtg ccgatttgcc
     2581 ggtcccggtg atctaggatg tcgaaaccct ggaggggggc gagacgttcc agcgctttgg
     2641 cctgcatgcc ccggacgagt tccttcaggc cttcgggggt gagatccctc tttttccaga
     2701 gatcggacgc gagcgtccag gaggcgtcga tgcccatcag ggcgttggga tggacgtcgg
     2761 agccgctgat gtagtacgtc aggtcgggct ggacctggaa ggcttcatag gccgccgacg
     2821 cttccgcgct cggtctcagc tttccgtagg aaccgccgga gaaccgccaa acggcgacca
     2881 caagcgcgac cgccacggcc aaaagtgcga gaacgacttg aagattcatg agcctcctgc
     2941 cgggcggggg cggccgtccc ggcgttgagg ggacggccgc ccccgcccgg ccctcatcgc
     3001 atctttccct gatcggcgac ggcgcgggcg gcggcctgga ccttgtccgg atcgcccagg
     3061 taagcgtggc ggagcggctg cagcgcctcg tccagttcgt agacgagggg gatgcccgtg
     3121 gggatgttca agccgacgat ctccgtctcc gatatgcggt cgagatacat gacgagggcc
     3181 cgcatgctgt tgccgtgggc ggcgatgagc acccttttac cttggcggat ggccggggcg
     3241 atttcatcgc gccaaaaggg catcacccgt gcggctgtgt ctttcaggga ttcggtgagg
     3301 gggagttccg cggcggtcag gtcccggtac ttgagttcgt ggccgggata gcgcgcgtcc
     3361 tccggggtca ggtccggtgg gggaacttcg tagctgcgac gccagagctt gacctgcgcc
     3421 tcgccgtacc ggacggcggt ttcggccttg ttgaggccct ggagcgcacc gtagtggcgc
     3481 tcgttcaggc gccaggactt cacgaccggg atccactcca ggtccatctc ctcaaggacg
     3541 atgttcaggg tgcggatggc ccgcttcagg acggacgtga aggccagatc gaagaggtag
     3601 ccctcgcgtt tcaggagccg tcccgcttcc agggcctcgt tcttgccggt ttcggacaga
     3661 tcgacgtccg tccagccggt gaagcggttt tcccggttcc agagactttc gccgtgacgg
     3721 agcagaatca gggtcggcat ggttaatcct ttcttgagat ttttgcttca tgttatttcg
     3781 gggagcgcgg atgacgagac atcttcacgg agacttttgc atcatgtcat ttcgaggagc
     3841 gtgagcgacg agaaatcttc aggatggcgg tttcttaaag atttctccct tcggtcgaaa
     3901 tgacaaaaca ggcggtggtg caaaagttcc ttcttgaggg tgcatccgtg atgatgcctc
     3961 gcggacggcg gggcgtcccc agcgatccta accgtctcta tatcagggcg ggggcgggat
     4021 gtcaaaatgt cctcgcggct ccgggagtta gggtccgggg ggcgggcgcc cggttttcga
     4081 agatctccga ttgtcatccg gccccgtttc tgctatccat gaataccggg gttggggaag
     4141 cgtccggaca aaccggcgaa cgttcctgcc cgtcttaccg gttcccggga tgtccgcgag
     4201 gaaactcaga tcgccatgac aaacgcgcgc aaagccgaac agggtccgtg ccgggcaacg
     4261 tttccgcatg gcggcggcga gcgccggatc gtcgtgatcg gggccggagc ggccggcatg
     4321 atggcggccg gccgggcggc ggagcggggg gccgaggtgc tcctctgcga gaagacggaa
     4381 caccccggca agaagctcct cgtgagcggc aagacgcgct gcaacctgac caacgcccgg
     4441 gagctcgacg gcttcatggg gatgtacgga ccggggggtc cgtttctcca tgccgccttc
     4501 cgccggtttt tccgcgagga cctgctggcc tttctcgaac gctatggtgt cgagaccaag
     4561 acggagcggg gcgggcggat cttccccgtc tccgatcgcg ccgaggacgt gcttgcggcc
     4621 ttccgcgcgt atctgaagga acagggcgtc cggctgcgga cggggtcgcc ggtgaccgcg
     4681 atccggaccg aggcggcgcg ggtgactggc gtccaaacct cccgggacct ctaccccgcg
     4741 gcagcggtca ttctcgcgac cggcggggcg acctggccgg cgacgggttc gacgggcgac
     4801 gggtatcgcc tggccgcggc gctgggacac cgcatcgtcc cgctccgtcc ggcgctggtt
     4861 cccctggcgg tgcgggagat ttcgctcgcc cgcggcctgc agggggtgag cctccggaac
     4921 gtccgcctga cggccttccg ggggccggcc gacgggatcg atcccgggcg gattccggaa
     4981 aatgaatgtg gccgcgggat cggcggccgc aagccgcgac cgccggtgat cgagagccgc
     5041 atgggcgaga tgatgttcac ccatttcggg atcggcggcc ccatcgtcct gctcatgagc
     5101 cttgccgtgg tcgacgccct ggtggcgggg ccggtcggcg tttcgatcga cctgaaaccg
     5161 gcgctgacgc cggcccggct tcgggagcgc ctgcagcgcg atttcgaccg gcacggccgc
     5221 cggcagttcc ggcgccatct ggaggggctc ctgccggcga agatggtggc gcccttcctg
     5281 gcgctgacgg acattcccgc cgacaagccg gggcatcaga tcgccgcttc cgaacgggac
     5341 cgtctggcgg gcctgctcaa gtccctccgc ttcaacgtca aggcccccct ccccatggcg
     5401 tcggccatcg tgacggccgg ggggatcgcc ctggaggagg tcgatccccg cacgatggcc
     5461 tcccggaagg tctccggcct tttcttctgc ggggaggttc tggatatcga tgccgatacg
     5521 ggcgggtata accttcaggc cgccttttcc acggggtatc tcgcggggga aggcgccgcc
     5581 gatgtcgtct tgtccccgga tgtgtccgcc cgggaggggc atggccggga aaggaccggg
     5641 atcgaggtcg tagggcaaga acctgaaacg ttcttgagaa gggagggagc gtcatgaaat
     5701 ggctgaaggc gggcgtcgtt ctggccgtca tcctgatcct cgccgttgtc gccggcggga
     5761 tgatctgttt gcgcatcaac cctgtccagc gggagggaga aatccgtctg tccggtctcc
     5821 aggcgccggt ccgggtggtg cgtgatggga acggcacccc ctacatttac gcgcggaccc
     5881 tggacgacgc gctcttcgcc caggggttcg tgacggccca ggaccgtctg ttccagatgc
     5941 agctcacccg gatgttcgcc gccggccgga tcgccgagat ggtgggggaa aaggcgcgca
     6001 acctcgatat ccgcatgcgg acgatcggat tcgtccgcca ggcccggcgg cacgaagcga
     6061 tcctcgatgc ccggacgcgg cgttttctgg ggcgctacct ggagggcgtc aacgccttca
     6121 tccaccaggg acgggatctg ccggtcgaat tcaagctggc cggcatccgg ccggagcctt
     6181 gggaaatcgc ggattcgctg tccgtcatct acctcatggg ctgggactcc gcggccaacc
     6241 tcaagagcga gatcgtggcc cggatgctgg tcgagcgcct cggcccggag aaggcccggg
     6301 agatctttcc cctcaacatc aatcccgacg agcgcctcgg gaagaccggc gccgtcttcc
     6361 ggaaaccgtc cccgcaggag gtgcggcggc agatcatcct gtccggggac gcggggctca
     6421 tcgccttcct gaaggacgtc aggccgctcg cggccggcag caacaactgg gccgtgggcc
     6481 cgaagcggtc ggccagcggc aagcccatcg tggccaacga tccccacctc gatgcccgga
     6541 tcctgccggg gccctggtat ccctcggggc tcttcacgcc ggagttccgc gcagtgggcg
     6601 tcacgatccc cggtgtgccg ggaatcgtcg ccgggcggaa cgaccggatc gccatcggca
     6661 tgacgaacgc ctacggcgac gcccaggatc tcttcatcga aaccctcgat cccaaggatc
     6721 ccggacggta cctggagggg cgcgtctccc ggccgtttca ggtcgtcccg gagacgatcc
     6781 gcatccgcga caagaaggcc cggggcggct ttcgggagga aacggtccag gtgcggctga
     6841 cccggcgggg tcccgtgatc agcggcctgt ttcccggtct cgaggcggag aaggcggaga
     6901 aggcggagaa ggcggtgacg gtccgctggt cgcccttcga gacgatgggg ccctccatcg
     6961 ggctggagcg gctcttcacg gcgcggtccg tcgggcaggt ccggcaggcg cttgggaacg
     7021 tcacgacgat catgctcaac ttcgtcttcg ccgacgtgga cggccatatc ggctggcaga
     7081 cgaccgggcg gctgcccatc cgcgcccggg gggacggtct tttgccggtc gaggtcaggg
     7141 acgggagcga cccctggcgg ggctggatcc cgtacgcgca aatgccggcc cgctacgatc
     7201 cgccccgcgg ctggctcggg acctgcaatc acatgacggt gacccgggat tacccgtatg
     7261 cctattccac ccatctgtcc ccgtcgttcc gctaccgtcg cctggcggag ctgctggacg
     7321 cgccgggaaa ggtttcggcc cacaaaaact ggctctacca gcgggatatg atgaacctcc
     7381 tggcccgcga cgtcgccccg gtcatggcgc gggtcctcct cggcgcgaag gagaccgaga
     7441 agatgggccg gattctgaac aagtgggatt attacgacta tgccggccga agcgggcccg
     7501 cgatcttcca tgccgtctgg atcaagtttg ctcggcgggt cttccaggac gagctcggcc
     7561 cggaactggc cggactgatg ctggacaact ggtatttctg ggaggagcgc ctccgggcca
     7621 tggtgctcgc gggcgaatcc ccctggttcg acgatgtgtc aacgccgcgg cgggaaacga
     7681 tggcggacct cttccgggag gcgggacggg aggccgcgca cgagcttaag gatacgctcg
     7741 gagacaatcc cgcccgctgg cagtggggga agctgcaccg gatcgagttt gtcagcccgc
     7801 tccgccggga gggcttcggc cgggggctgg tgggcggcgg atcccatccc atgggcggtt
     7861 ccccggagac cctctatcgc gggatctacg atttcaacaa gccctacgcg gtgaacgtct
     7921 cggcctcgct ccggatggtg gccgacctgg gggatccgga ccgggtctac gccgtcctgc
     7981 ccggcggcgt gtcgggacgg cttttcgacc ggcacaaccg ggatcagatc cgtcccttca
     8041 tggatggcga gaagcgggcc tggtggttca gcgacgccat gattgagaag catgcgaagt
     8101 atcggctgga actgaaaccg tgaatcataa ggaggtcacg atggaactgt tcgaaacgat
     8161 ggagacccgt tcgagccgtc gggcgttttt atcccggccg gtggagaggg acgtgctgga
     8221 gcgtctgatc gcggcggcca accgcagccc ctcataccag aacacccagc cctgggaggt
     8281 gttcgttgca acgggagaga aaaaggacgc ggtggccgcg aggatgcacg acaacgtcgt
     8341 gaaggggacg ccgatggagc cgcacatccc cttcgcccgg gttctaccga aggcccatga
     8401 aacccgggcc ctgtcgcacc ggaaccggcg cttccaggcc ctggggatcg atccggagaa
     8461 ggaccaggag cggattaccg agagctacat gagaaacttc atgttcttcg gcgcgccctg
     8521 cgtgatcttc gtcgggatgg acaagaccct gtccccctgg tcgattttcg accttggcgc
     8581 gttcgtgcac gcatttctgc ttgccgccca cgccgccggc ctgggggcgg ttccgcaggc
     8641 aatgcccacg ggctatccgg cgatcgttcg cgaggagctg gggatttcgg aggatatcgc
     8701 gatcatcctc gccctgtcgg cggggtatcc ggacctcgac gccccggtga acggttaccg
     8761 gtccctccgg cgtgaaaccg tggaattcgt ccgctggatc gggttctaaa cgttcaggag
     8821 ttcgctcagg ctcgggtggg ggaggatcca gttcgccagt tccctccgtc cgagattcgc
     8881 ggagacggcc agcgccaggg gggcgatgag atccccggcc gcctcgcccg cgaccgcggc
     8941 gcccgcgaag gtcccccggt ggaagagcag cttggcgaag cccgtgggaa cgagttccgc
     9001 gcgggccatg atattcgcgg cgtagtccgg ccgcacgatc tcgatctcga tcccgcgggc
     9061 ggcggcctcc tgctccgtga ggccaacccg ggcgatcgga ggatgggtgt agatgaccgc
     9121 cggcgccgcc cgctcgtcgc aggaaatcgt gccggcgccg aagaggcgat cggccagggc
     9181 gcgcccctgt tgcatcgccc gatgggcgag catcatcccg ccggtcacgt ccccgacggc
     9241 ctggatgccc gggaccgtcg tctgctggcg ggtgtcgacc cggattccct ggaggccgta
     9301 atcgatccca aggcgcttca gggcgtcttc gtccaggctg ggtctgcgcc cggcggcgag
     9361 aaggacgcag gtcccccgcc attgaagcgc cggtgtttcc ccgtcccgtt cagccggttc
     9421 ggcggtcagc gccaccccgt cttcgtcctc ctcgatcgcc gtcatccgaa cacccgtgcg
     9481 gatctcgacg ccgagtcgct tcagttcctg tgcgagaaat cctcccagat ccggatcctc
     9541 cgtcggaatc agacgatcca ggagttccca gagcgtcacc cggcagccca gttccgccag
     9601 gagcgtcgcg aactccaccc cgatcacgct gccgccgacg atggcgatgc gctcgggcaa
     9661 attcgggagg gccaggaggc cgtcggaggt caggatgcgc gccgaaggga cgaaaccggc
     9721 cgggagcagg ggtgacgaac cccaggcgag gcagacgttt tttgcatgca gggtttcacg
     9781 gcggccgtcc ggcgtgacgc agtcgacctc gccgggggcg cgcaggcgtc cctcgccctc
     9841 cttgagcacc gcaccggcgg cctcgaggga cttgcggacg cccagggcgg agatccggac
     9901 cagttgcccc tggtggcgct tcatggcggc gaagtcgaag gaggcttcgc cgatcccgat
     9961 tcccaggcgc ccgaggcgct tcagggcggc gtaatggccg ctgcaggcca gcagcgcctt
    10021 cgtcgggatg cagccccggt gggtgcaggt tccgccccag ccgtcccgtt cgatcgcgca
    10081 gacccgcgcc ccgaggcgcg ccgccgtctc ggccgcggcg tggcccccgg gccccccgcc
    10141 gatcacgatc aggtcgaacc gttcttccat gcgacccgtt ccctttgcgc ggcggatgac
    10201 cgaacgcccg tccgcgcgtc gatccggcat gcggtcggat cctatcccgg cgcgccgggc
    10261 gccgtttcgc tgacggtcac ccgctccatg cagtcgccct ggcggatggc gttcacgacc
    10321 gcctgcccgc tcacgacctt tccgaacacg gtatgctttc cgtccaggtg cggctgcggg
    10381 gaatgggtga tgaagaactg gctcccgttc gtgtccggcc cggcgtttgc catcgagatg
    10441 acctggctat cgtggcggag cgggttgccc cggcattcgt cctcgaaccg gtatccgggt
    10501 ccaccccggc ccgttcccgt cggatcgccc ccctggatca cgaaatccgc gatcacccgg
    10561 tgaaacgtca cgccgtcgta gaacccctct cgggcgagga agacgaagtt gttgacggtt
    10621 ttgggggcat gttccgggta gagctcaagc tcgatcgcgc cgcgacccgt ttccatgcgg
    10681 atgtgatacc gcttcccggg atcgatctgc atctccggcg gacgatccca ctgtttttcg
    10741 gccatcaggc gcctccttag cgtgtggttt gtgtgtgacg ggtccatccg gatggtccgg
    10801 gcggatagac acccatatca aatggggggc ggaatgcaaa ggagaattcg ccccgcccgc
    10861 ggggacaggg aagagccaaa agctcgccga cgtgcgcccg cgggatcgcc ttttcggatc
    10921 cgacgggatc gtggtttgaa gagggtggga atccgccggg ttttctgata atagaccttt
    10981 gggaatccgg ccccgaaacg gacccgtcgg tgcggggccg gccacggagc gtcgacctcg
    11041 acatgcaaac gtccggtgcg catgcgggga tggccccctt ttcatgacca tgcctcccat
    11101 tttttgcatc catcccgcgc gtgcgggatg gaatctccgg gggggcatgg cgtttcgtcg
    11161 ggacaacgga atttgtcccg gtgatcgggg aaacgggatg aattttccgg gaggagtgcg
    11221 gtgaaacagg ggaaaggaat caggggggcg atgcagcagc tgctggcggt cgtcctgctg
    11281 ctggcggcgg ccaccccggc ctccgcccag tggctggagg aggcggtccg agagcacacg
    11341 ctcgcgaacg gcatgaagct cctgatggtg gagcgccacg cctcgccgac ggtctcggcc
    11401 tggatccgct accgcgtggg aagcgcggac gagcgcagcg acgaacgggg cgttgcccac
    11461 ctcctggaac acatgctctt caaggggacg aagaccctgg gaacgaggga ctacgccgcg
    11521 gaaaggccgc ttctggagcg gatcgaggcg acggcccggg cgctcgtggc ggaaaaggcc
    11581 aggcgcgcaa aaggcgatcg ggagcggatc gcccgtctgg aggaggaact ggccgcgctt
    11641 gaaaaagagg cggcgcggta tacggtccgg gaggagtttt tcgagatcta cgcccggaac
    11701 ggcggcacgg gctacaacgc gttcacgggc aaggacggca cgacctatct ggtcagtttg
    11761 cccgccaaca agctggagct gtgggccgcc atcgaatcgg accggatgat gaacccggtg
    11821 cttcgcgagt tctacagcga acgggccgtg gtcatggagg agcggcgccg gtcctacgac
    11881 acccagccgg aggggcggct ctgggaaaat ttcctggcca ccgccttcgt cgcccatccc
    11941 tacggccagc cgatcatcgg ctggatgtcc gacctggaga acctgacccg gacgcgggcg
    12001 gaggccttcc tgcaccggta ctacgccccc aacaacgccg tggtggccgt ggtcggggac
    12061 atcgacccgg accggacgat tgccctcgtg gaacgctatt tcggtgccat cccccccggc
    12121 gtcgatgtgc cgcccgtggc cgtggacgaa cccccccagg ccggcgagaa gcggatcgaa
    12181 cttcgcttgg actccgagcc ccacctgctg gtcgggttcc acaaaccggt ctatccgaac
    12241 cccgatgacg acgtgttcga cgtcatcgag atgatcctgg cggaaggcaa gac
//
LOCUS       DAOOLJ010000057        12247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_5735_length_12247_cov_14.307579, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000057 DAOOLJ010000000
VERSION     DAOOLJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12247
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5735_length_12247_cov_14.307579"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLW16_08340"
     CDS             complement(<1..540)
                     /locus_tag="PLW16_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB70954.1"
                     /translation="MKSSILIVDDETLLLESLKKALSREGYVALTATTGREALRCFEE
                     HAPDLVLLDVKLPDIDGIQVLQRLRKVDTQTPIIVMTAYSGIKGAVEAIKFGAYDYIA
                     KPFDVDELKFVVARSLASRRVEAEVDQIRTTKKERYSFANILTVNERMKQIIKLGQRV
                     ANNSQSTVLISGESGTGKEL"
     gene            complement(551..2560)
                     /locus_tag="PLW16_08345"
     CDS             complement(551..2560)
                     /locus_tag="PLW16_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB70955.1"
                     /translation="MKFVRLFEPIVINRLFIENRTVMPAMGLHYTNDYSFPERMRHFY
                     RRRAHGGVGLMILGPVAFDERGAAPAMPGIFDDRIVEAFRVFLDELHRDTKTKIAIQL
                     FHMGRNAPSPLYTGKPALAPSALKSRLTGLVPQAMTEEDIREVQVGFATGAKRAKDAG
                     FDMAEIIACTGYLVSQFLSPVTNLRTDAYGGPIENRMRFGLEVIRTVRAAVGPDFPIG
                     IRVAGNDFMEGGHTNAESALFCREAERAGVDAINVTGGWHESNTPQLTSHVPPAAYVY
                     LARGIKEQVGVPVFASNRLGDPIVAEQVLRSGAADMVGWARPLLADPDLPRKVRDGQA
                     ALAIRCICCNQGCFDGIFAGRGVTCLVNPTVGREGELKIAPARPPKKIYVAGGGPAGM
                     EFAWVAALRGHDVTLFEKEERLGGQVDLAAAPPGKAEFLNVTRSLEARMRAAGVKIRR
                     RSPLTAAKVRRGEPDLVAVATGSRPITVGVPGADRPHVVDAWDVLRGNVAHIGRRIVV
                     IGGNAVGCETADFLVSADCPKPENAAFLLYHGAENPQHLRELLCRSGRQVTVIEMVER
                     MAANVGPSTRWVLLKDLQMKGVELRTGTKLVEITDKDVVVETAAGRESIPADTVVMAV
                     GSRSVNDLARRIEQNGTPVVTLGDAASPRNMMDAIREGFEAALTV"
     gene            complement(2676..3161)
                     /locus_tag="PLW16_08350"
     CDS             complement(2676..3161)
                     /locus_tag="PLW16_08350"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HPB70956.1"
                     /translation="MIEKKYAGVARYVESGIDAIGRFGVKALEFRDRHVKLLMPLDRN
                     VNHVGMMYAGALFALGEVMGGAIFGVAFDYRKYFPIVREVQIRYLKPAFTDVTLTADI
                     SEAQVAEAVAAFEAKGKADIALDLELKDAEGTVVAQVRGVWQGRKLPEGFPVPWPTEE
                     A"
     gene            complement(3165..3971)
                     /locus_tag="PLW16_08355"
     CDS             complement(3165..3971)
                     /locus_tag="PLW16_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB70957.1"
                     /translation="MADPIHGEKKGPIFYLTLNRPEKRNAITFDMLADIAEMVEDLIT
                     DADIRVILLRGEGPVFSAGVDFPSLAGLVDRFLKDSAAGGASIRADISRYQQYLNRLE
                     TIEIPILCAIHGRLLGLGMELVLPCDIRLMSDDCIWSMPELKFGTIPDLGGTARLTRV
                     VGAAKAMEILMTGRMYPAAEALRLGLVNGLCPAADLMAEAEKMATDIAAMAPLAVGAV
                     KRVVRKGEGVDLMTHLDMEANLQSILLRSEDFREGVSSMMERRPPNWRRR"
     gene            complement(4063..5727)
                     /locus_tag="PLW16_08360"
     CDS             complement(4063..5727)
                     /locus_tag="PLW16_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70958.1"
                     /translation="MSEERIWQKAYMPGVPKTVSFEDRTMPEFLTRTARRFPRRDALI
                     YLGRRISFAELDRLADRFASALKAIGVKKGDRVALLLPNVPQIVIAYFGIWRAGAAAL
                     PCNPLYSDREIEHQVNLAGASVMITLDLFAPRMLALRSRTGIRQVIAAHINDYLPTPQ
                     RQLFPFVKRAMYLPYRKEDGYHDFMALMRAAPDQSNHTPPALDDLALIPYTGGTTGVS
                     KGAIISHRNISSINQILMAWFFDLPDGEDSELAIFPFFHMAGFNLVMNLSICKGWTAI
                     LVPRPDPQAVMDLTRKYRPTIFLAVPTIYVGVMALPEFRKSDLSFIKGFFSGAAPLLI
                     ETIEALKKATGASIVEGLGMTESTSIITMTPWRGKLKPGSVGVPLPNTDVKIVDLETG
                     ETDLPPGAEGEICFRGPQMCGGYYGMPEETERAVRGGWFYSGDIGRMDEEGYIYVVDR
                     KKDMIIAGGYNIYPREIDEVLYEHPGVLEACTVGVPHEYRGETVKAFIVVKPGVQLNE
                     KELDAFCRERLAAYKVPKLYEFTDNLPKSAVGKILRRELRDRAAGA"
     gene            complement(5898..7574)
                     /locus_tag="PLW16_08365"
     CDS             complement(5898..7574)
                     /locus_tag="PLW16_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB70959.1"
                     /translation="MAMQRPWFKSYHPNVPHEIEIEKITMPEVLQRTADRHPDGIALN
                     YFGKRITFRQLNGLVNRLANALLAMGIKKDDKIAVLLPNIPQSAVANLAAMRIGAVPA
                     MLNPLYTERELEYQINDAEAKMAITLDLLLPRIQTLKTKTGIGPIVTCHINDYLPFPK
                     KQLFPYAKKGMYRKIDPQPEVFEFMDLMEGYPDTPVRNEARWDGIAVLIYTGGTTGVS
                     KGVMLTQSNISSNVQQFAAGFHDVKVGQGLMFIFPFFHSAGHLCMHLCLWMGLENVLI
                     PRPEPPILAELIKSAKPTYIGAVPTIYVGLLALEKFSKMDLSFIGGFVSGAAPLPVEI
                     IEQLKRLTGSTMLEVYGLTETTPVVTATPWKGKIKVGTVGVPVCNTDMKIVDVDTGEK
                     ELPPGEAGELCFKGPQVMKGYYKKPEETAMVLKDGWLYTGDIGVMDEDGYVSIVDRKK
                     DVIIAGGFNIYPKEIDEILFEHPKVLEACTIGIPDSYRGETVKAYIVAKSGQTLTTEE
                     VTAFCKERLSPYKVPKQIEFIDELPKSAVGKILRRELRDLDKKKRESRQA"
     gene            complement(7652..8803)
                     /locus_tag="PLW16_08370"
     CDS             complement(7652..8803)
                     /locus_tag="PLW16_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70960.1"
                     /translation="MSVLEKHYTQEHQIFREAVRRFYEKEITPNAEAWEEAGMVPREL
                     WRKYGEQGFLCPWLPEEYGGVGADFLYSVIALEELAPCHMSGFVAFLHSDVVVPYLYS
                     FGTEEQKRRWLPKCVTGETITAVAMTEPGTGSDLAAIRTTAVRDGDHYVLNGQKTFIS
                     NGLLCNLVVVAAKTDVQAKPPYAGISLIVVEEGTPGFEKGRKLDKVGLHSQDTAEMAF
                     VDCRVPVENLLGSEGQGFHFLMQKLQQERLMSAIGAQATAEEALRLTIEYTKSREVFG
                     RPVSRFQYIAFEMAKMATEVELGRAFLDSLILEHMEGRDIVKKVSMAKYWISEMLNRV
                     VGQCVQFHGGYGYMEEYPIARLFRDARVHTIFAGTSEIMLLIISRYMGL"
     gene            complement(8891..9367)
                     /locus_tag="PLW16_08375"
     CDS             complement(8891..9367)
                     /locus_tag="PLW16_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB70961.1"
                     /translation="MTETKKDAQELLMVTSGDSSQPFNWTVDEYGSTFLREMRDHKKL
                     VGIKCPKCGKVYVPPRPVCGQCYVGMNEIVPVSDEGEIYVFTIVHFGFVDPNTGVQRP
                     VPHGYAFIKLDGADTCLTHFLDSVDPVKVKVGARVKAVFEPKRTGSMMDIKHFKVL"
     gene            complement(9486..10679)
                     /locus_tag="PLW16_08380"
     CDS             complement(9486..10679)
                     /locus_tag="PLW16_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70962.1"
                     /translation="MGKRVAVVGYGQTYQRSSRKDVNGVELINEAVRVALADAELTIK
                     DIDAIVIGNMDHFEGINYVDMWSAEGSGAVMKPVMKVTTGGTTGTSVAMCAYYHVASG
                     MFDKVLAIGWEKNSESDTTGAIITAFHPVWERPNLGGAVAGLAIEASKYMHDYGATEK
                     DGARVAVRERLHALNNPYAHLHQEWMKGIPPDQLVDLIVGSEQNQLLAYPLRMSDMCP
                     RTDGAAAVIFAVEGKAEKIAPIPAWVQATANRHNFVYAGDVDMARNTTIAEASQAVFR
                     KAGIKEPLKQIDVCELYLPYSYAGLKWIEDIGFCGYGEGPRLLWDGVTDMDGEMPINP
                     SGGVMSSNAIGATALIRVGEGALQVMGRAGARQVNRKVNLAFCSGFGGCYWADIMLLG
                     RKKPA"
     gene            complement(10690..11826)
                     /locus_tag="PLW16_08385"
     CDS             complement(10690..11826)
                     /locus_tag="PLW16_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPB70963.1"
                     /translation="MENIAIVGVGQTVCARRREETFEQLVFEAATKALADAGAEIGDI
                     DNVVTVSNDFWDGRTISSMAVQEAAGGWHKDISTVEGDGAFGAFYGVMRILSGSFDTA
                     LVVGHIKGSEGVMNLITNAMFDPIYQRSLGIDATAAAALQARIYMQMSGATERDFARV
                     SVKNHGQAMKNPFAQLPMKLTLKDVLESRPIAEPLKLLDCSPISDGAAAVVLSNRRGL
                     KRFKKDPVWIRGVGHCTDAFNLGYRDLARSRSLGEAAKKAYKMAKIKDPLKELDVAEL
                     YDAYSYMELMWYEGLGLAPEGEGKQLIREGATALGGVLPVNPSGGVLSGHPVIVAGLI
                     RLIEATLQVRGEADKRQVKGAKTALAQGINGPCGQAHCVFIVGA"
     gene            complement(11847..>12247)
                     /locus_tag="PLW16_08390"
     CDS             complement(11847..>12247)
                     /locus_tag="PLW16_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPB70964.1"
                     /translation="YAWSVGATGSRFLTALRDDQEIWGVKCPKCKRVYVYPIKNCGEC
                     FVPTEQWVKVANVGTLESFTVVHYKHDMQPRKRPLIYGLVKLDGTDGALLHLIGGVEP
                     AKIKLGMRMKAVFARQRAGSILDIQHFAPA"
BASE COUNT         2130 a         4051 c         3723 g         2343 t
ORIGIN      
        1 cagctccttc ccggtcccgc tctccccgga gatcagcacc gtggactggc tgttgttcgc
       61 gacccgctgc cccagcttga tgatctgctt catccgctcg ttgaccgtca ggatgttggc
      121 aaagctgtag cgctccttct tcgtggtgcg gatctgatcg acctcggcct caacccggcg
      181 ggaggcgagg gaacgggcca cgacgaactt cagttcgtcg acgtcgaagg gtttggcgat
      241 atagtcgtag gccccgaact tgatggcctc gaccgccccc ttgatgccgc tgtaggccgt
      301 catgacgatg atgggcgtct gggtgtccac cttgcggagg cgctggagga cctggatgcc
      361 gtcgatgtcg ggcagcttga catccagcag gaccaggtcg ggggcgtgct cctcgaaaca
      421 gcggagggcc tcccggccgg tcgtcgccgt caacgcgacg tatccctccc gggagagggc
      481 cttcttcagg gactccagaa gcagggtttc gtcatcgacg atcagaatgg acgatttcat
      541 gacttcaccc tcacaccgtc agggccgctt cgaagccctc gcggatcgcg tccatcatgt
      601 tccgggggga tgcagcgtcc ccgagggtca ccaccggcgt cccgttctgt tcgatccgtc
      661 gggccaggtc gttgacggac cgggacccga cggccatgac caccgtatcg gccggaatgg
      721 attcgcgacc cgcggccgtt tccacgacaa cgtccttgtc cgtgatctcg acgagcttcg
      781 tgcccgtgcg cagctcgacc cccttcatct gaaggtcttt caggagtacc cagcgcgtcg
      841 agggccccac attggcggcc atgcgttcga ccatctcgat aaccgtgacc tgacggccgg
      901 aacggcagag cagctccctc aggtgctgcg ggttctcggc cccgtggtac aagagaaacg
      961 ccgcgttttc cggtttcggg cagtctgccg agaccaggaa atcggcggtt tcgcacccga
     1021 cggcattccc gccgatcacc acgatgcggc ggccgatatg ggcgacattc ccccgcagca
     1081 cgtcccaggc gtccacgaca tggggcctgt ccgcccccgg aacgccgacg gtgatcggcc
     1141 gggaaccggt ggcgacggca accagatcgg gctccccccg cctgaccttg gccgccgtca
     1201 ggggcgacct tcgccggatc ttcacgcccg ccgcgcgcat ccgcgcttcc aggctccggg
     1261 tcacattcag aaattccgcc ttcccgggag gcgccgccgc cagatcgacc tgcccgccga
     1321 gccgctcctc cttctcgaag agggtcacgt catggccgcg cagcgcggcg acccaggcga
     1381 actccatccc cgcgggaccg ccgccggcca catagatctt cttcggcggc cgcgccgggg
     1441 cgatcttgag ttccccctcc cggcccaccg tgggattcac aaggcaggtg acgccccggc
     1501 cggcgaagat gccgtcgaag caaccctggt tgcaacagat gcagcggatg gccaatgccg
     1561 cctgcccgtc ccggaccttc cgcgggagat ccggatcggc caagaggggc cgcgcccagc
     1621 cgaccatgtc cgcggcgccg gagcgtagaa cctgctcggc cacgatgggg tcgcccaggc
     1681 ggttcgaggc gaagaccggg acgccgactt gctccttgat cccccgcgcc aggtagacgt
     1741 aggcggcggg cggcacgtgg gaggtgagct gcggggtgtt ggactcgtgc cacccgccgg
     1801 tgacattgat ggcatcgacg cccgcccgct ccgcctcccg gcagaacagg gcggattccg
     1861 cgttcgtgtg tccgccttcc atgaagtcgt tccccgcgac ccggatcccg atggggaaat
     1921 ccggacccac cgccgcccgg accgtccgga tgacctcgag accgaaccgc atccggtttt
     1981 cgatgggtcc gccgtaggca tccgtacgga ggttcgtgac cggggacaga aactggctga
     2041 cgaggtagcc cgtacaggca atgatctccg ccatgtcgaa gcccgcgtcc ttcgcgcgct
     2101 tcgcccccgt ggcaaaaccc acctggacct cgcggatgtc ctcctccgtc atggcctgcg
     2161 gcaccagccc ggtcagtcgg ctcttcaggg ccgagggggc cagggcgggc ttccccgtgt
     2221 agagcggaga cggggcgttc cgccccatgt ggaagagctg gatggcgatc ttcgttttcg
     2281 tgtcgcggtg gagctcatcc agaaagacgc ggaacgcctc cacgatccgg tcgtcgaaga
     2341 tgccgggcat ggcgggcgcc gccccgcgtt cgtcgaaggc gacgggaccg aggatcatga
     2401 gtcccacccc gccgtgcgcg cggcggcggt agaaatgccg catccgctcc ggaaacgaat
     2461 agtcgttcgt atagtgcagg cccatggcgg gcatgaccgt gcggttttca atgaacaaac
     2521 ggttgatgac gatgggttcg aacagcctga cgaatttcaa tccattctcc ttgactcacg
     2581 aacggccctg ccggtcgagc ccggcgagac ttgaatccat gggttagctg accgccaccc
     2641 gcagtcggga cgacggcgac cggcgcggcc gggggtcacg cctcctcagt cggccaggga
     2701 acgggaaagc cttcgggcag cttccgcccc tgccagaccc cccgcacctg ggcgacaacg
     2761 gttccctcgg cgtccttgag ttcgaggtcc agggcgatat ccgccttgcc tttcgcctcg
     2821 aacgccgcaa ccgcctcggc cacctgcgcc tctgaaatgt cggccgtcag ggtcacatcc
     2881 gtaaacgccg gcttgaggta acggatctgc acctcccgga cgatggggaa atattttcga
     2941 tagtcgaagg ccacgccgaa gatggccccg cccatcacct cgcccagcgc gaaaagcgcc
     3001 ccggcataca tcatccccac gtgattgacg ttgcggtcga ggggcatgag gagtttcacg
     3061 tggcgatccc ggaactccag ggccttcacg ccgaaccgac cgatggcatc gatcccgctc
     3121 tccacatacc gggcgacgcc cgcatatttc ttctcgatca tccgttacct cctccgccag
     3181 ttgggaggac gccgctccat catggacgac accccttccc tgaaatcctc gctccgaagc
     3241 agaatgctct gcagattggc ctccatgtcc agatgggtca tgagatcgac cccctcgccc
     3301 ttccggacga cccgcttgac ggcgccgacg gcgagcgggg ccatggctgc aatgtccgtc
     3361 gccatcttct ccgcctccgc catcagatcc gcggccgggc agagcccgtt gacgagaccc
     3421 agccgcaggg cttcggccgc cgggtacatc cggcctgtca tcaggatctc catcgccttt
     3481 gccgccccga cgacacgcgt caggcgcgcg gttccgccca ggtcggggat ggtgccgaac
     3541 ttcagctccg gcatgctcca aatgcagtcg tcgctcatca ggcggatgtc gcacggcagg
     3601 accagttcca tgcccagccc cagcaaccgg ccgtggatgg cgcacaggat cgggatctcg
     3661 atggtctcca ggcggttgag gtactgctgg taccgggaga tgtcggcccg gatggatgcc
     3721 cccccggccg ccgaatcctt gagaaagcgg tcgaccaggc ccgccaggga cggaaaatcc
     3781 acgccggcgg aaaacacggg gccctcgccg cgcagaagga tgacccggat gtccgcgtcg
     3841 gtgatcaggt cctccaccat ctcggcgatg tccgcgagca tgtcgaacgt gatcgcgttg
     3901 cgcttttccg gtcgattcag cgtcagataa aagatcggcc ccttcttctc tccgtggatc
     3961 ggatctgcca tcgctccctc ctgccgtaaa aaaatttgcc cccttatagg aatttttctc
     4021 cccggcgtca atcggtgttt cccggattcc ggggggattc ggtcacgccc ctgcggccct
     4081 gtcccgcagc tcccggcgga ggattttgcc cacggcgctc ttgggaaggt tgtcggtaaa
     4141 ttcatacagc ttcggcacct tgtaggcggc caggcgctcg cggcagaagg cgtctagttc
     4201 cttttcgttc aattgcacgc cgggtttgac aacgatgaag gccttgacgg tttcgccccg
     4261 gtattcgtgg gggacgccga cggtacaggc ttcgagcaca cccgggtgct cgtagaggac
     4321 ctcgtcgatc tcccggggat agatgttgta gccccccgcg atgatcatgt ccttcttgcg
     4381 gtcgacgaca tagatatacc cttcttcgtc catccggccg atgtcccccg aatagaacca
     4441 gccgccgcgg acggctcgct ccgtctcttc cggcatgccg tagtacccgc cgcacatctg
     4501 cggcccccgg aagcagatct ccccttccgc gcccggggga agatccgtct cccccgtctc
     4561 cagatcgacg atcttcacgt ccgtgttcgg gaggggcacg ccgacgctgc ccggcttgag
     4621 ttttccccgc cagggcgtca tggtgatgat ggaggtcgat tcggtcatcc ccaagccctc
     4681 cacgatgctg gcaccggtgg ccttcttcag ggcctcgatc gtctcgatga gcaggggcgc
     4741 ggccccggaa aaaaacccct tgatgaacga caggtcactt ttccggaatt caggcagggc
     4801 catgaccccc acgtagatgg tcggcaccgc gaggaagatc gtcggccggt atttccgggt
     4861 gaggtccatc accgcctggg gatcgggacg gggcacgaga atggccgtcc agcccttgca
     4921 gatgctcagg ttcatgacca ggttgaaacc ggccatgtga aagaagggaa agatggccag
     4981 ttcgctgtct tcgccgtcgg gcaggtcgaa aaaccaggcc atgaggatct ggttgatcga
     5041 ggagatgttc cgatgggaaa tgatcgcccc cttcgagacg ccagtggtcc cgcccgtgta
     5101 ggggatcagg gcgagatcgt ccagggcggg gggcgtgtgg ttcgactgat cgggagcggc
     5161 gcgcatcagc gccatgaaat catgataccc gtcttccttc cggtagggca ggtacatggc
     5221 acgcttgaca aaagggaaaa gctgccgttg cggcgtcggc agatagtcgt tgatatgcgc
     5281 cgcaatcacc tgccggatgc cggtgcgcga cctgagcgcg agcatccggg gcgcgaagag
     5341 gtccagcgtg atcatcaccg aggcgcccgc caggttgacc tggtgctcga tctcccggtc
     5401 cgagtagagg gggttgcagg gcagcgccgc tgccccggcc cgccagatgc cgaagtaggc
     5461 gatgacgatc tgggggacgt tgggcaacag cagagccacc cggtcgccct ttttgacccc
     5521 gatggccttg agggcagagg cgaaacggtc ggccagccgg tccaattcgg caaacgaaat
     5581 ccgccggccg aggtagatca gggcgtccct gcggggaaag cggcgggccg ttcgggtgag
     5641 aaattccggc atggtccggt cttcgaagga gaccgtctta ggaacaccgg gcatatacgc
     5701 cttttgccag atccgctctt cgctcaaggg gtcctccttg tcctggatgc aaaatcccgt
     5761 gaaagcgctc cccgtcccgg tcccgcctgc acgggcgaaa atccgggcag atcatggaag
     5821 aacggggttc acgtctccgc gggcatgatg tctccccgaa tcccggaggt ctgcgaacat
     5881 gcgcttgccc gcgttcctca agcctggcgg gattccctct tcttcttgtc caagtcccgg
     5941 agctcccgcc ggaggatctt gcccacggcg ctcttgggca gttcgtcgat gaattcgatc
     6001 tgcttgggaa ccttgtacgg gctcaaccgc tccttgcaga aggcggtaac ctcctcggtc
     6061 gtcagcgtct gtccggattt ggcgacgatg tacgccttga cggtttcacc ccggtaggaa
     6121 tccggaatgc cgatggtaca ggcctcgagg accttgggat gctcgaagag gatctcgtcg
     6181 atttccttag gataaatgtt gaatccgccg gcgatgatga cgtccttctt ccggtccacg
     6241 atcgacacgt agccgtcctc gtccatgacg ccgatgtccc cggtgtagag ccaaccatcc
     6301 ttgaggacca tcgcagtctc ctcgggcttt ttgtagtagc ccttcataac ctgcggcccc
     6361 ttgaagcaga gctcgccggc ctcgccgggc ggcagttcct tctcgcccgt gtcgacgtcc
     6421 acgatcttca tgtccgtgtt gcagacaggg acgccgaccg tgccgacctt gatcttcccc
     6481 ttccaaggcg tcgcggtgac gaccggggtc gtctccgtca ggccatagac ctcgagcatg
     6541 gtggagccgg tcaaccgctt gagctgctcg atgatttcca ctggcagggg ggcggcgccg
     6601 gagacgaacc cgccgatgaa cgacagatcc atcttgctga atttctccag ggcgagcagc
     6661 ccgacgtaga tcgtcggcac ggccccgatg taggtcggct tggcgctttt gatgagctcc
     6721 gccaggatgg gcggctccgg gcgcgggatc aggacgttct ccaggcccat ccacaggcac
     6781 aggtgcatgc agagatgccc cgccgaatgg aaaaagggga agatgaacat caggccttgg
     6841 cccaccttga cgtcgtgaaa cccggcggca aactgctgca cgttggagct gatattcgac
     6901 tgggtcagca tgacgccctt gctcaccccg gtcgtgccgc ccgtatagat caggacggca
     6961 atgccatccc atcgggcctc gttccgaacc ggcgtgtccg gatagccctc catcaggtcc
     7021 atgaactcga agacctcggg ctgcggatcg atcttgcggt acatgccctt tttcgcgtag
     7081 gggaagagct gcttcttcgg aaacggcagg taatcgttga tatggcaggt cacgatcgga
     7141 ccgatgccgg tcttcgtttt gagggtctgt atccgcggca gcaagaggtc cagggtgatc
     7201 gccatcttgg cctcggcgtc gttgatctgg tattccagct cccgctcggt atagagggga
     7261 ttgagcatgg ccggcacggc gccgatccgc atggccgcca ggttcgccac ggccgactgg
     7321 ggaatgttgg gcaggagaac ggcgatcttg tcgtccttct tgatccccat ggccagcagc
     7381 gcgttggcca gccgattcac gaggccgttc aactggcgga aggtgatcct cttgccgaaa
     7441 taattcagcg cgatcccgtc cgggtgccgg tccgccgtgc gctgaagcac ctcgggcatg
     7501 gtgattttct cgatctcgat ctcatgcggt acgttcgggt gatagctttt gaaccagggt
     7561 cgctgcatgg ccatatcctt catcctttct gttgaacggg caacggttga gacgcccccg
     7621 gcgctgccgt cgggggtgcg gtgaacgagg attaaagccc catgtatcgg ctgatgatga
     7681 gcagcatgat ctccgaggtg ccggcaaaga tcgtatggac ccgggcgtcc ctaaacaggc
     7741 gcgcgatggg atactcttcc atgtacccgt agccgccgtg gaactgcaca cactgcccca
     7801 ccacccggtt gagcatctcc gagatccagt acttggccat cgacactttc ttgacgatat
     7861 cccgaccctc catgtgctcg agaatcaggc tgtccagaaa ggcgcggccc agttccacct
     7921 ccgtcgccat cttggccatt tcgaaggcga tgtactgaaa ccggctgacg ggccggccga
     7981 acacctcgcg gcttttcgtg tattcgatcg tgagccggag cgcctcttcc gccgtcgcct
     8041 gggcgccgat cgcgctcatc aggcgctcct gctggagttt ctgcatgagg aaatgaaacc
     8101 cctgaccttc cgaacccagg agattttcga ccggcacgcg gcaatcgacg aaggccatct
     8161 cggctgtgtc ctgactgtgc agaccgacct tgtccagttt tcgtcccttc tcgaaccccg
     8221 gcgtcccctc ctcgacgacg atcagggaaa tccccgcgta cggcggtttg gcctggacat
     8281 ccgtcttggc cgcgacgacc acgaggttgc agagcaggcc gttggagatg aaggtcttct
     8341 gaccgttcag aacatagtga tcgccgtccc gtaccgcggt cgtccggatg gccgccaagt
     8401 cggaacccgt accgggctcg gtcatcgcaa cggcggtaat cgtctcgccg gttacgcact
     8461 taggcagcca gcgtcgtttc tgctcctccg ttccgaagct gtagaggtaa ggcacgacaa
     8521 cgtccgagtg caggaaggcg acgaaacccg acatgtggca gggggccagt tcctcgagcg
     8581 cgatgacgga atacaggaaa tccgcaccga cgccgccgta ttcctccgga agccacgggc
     8641 agaggaagcc ctgctcgccg tacttccgcc aaagttcccg gggcaccatc cccgcctctt
     8701 cccacgcctc ggcgttcggg gtgatctcct tttcgtagaa ccggcgcacc gcctccctga
     8761 aaatctggtg ttcctgggta tagtgttttt ccaaaaccga cattcaaaat ctcctccgca
     8821 aaaaacagaa ggctcgggga tccggaggct cgacctcagc ctctctcgcc cctcgccttt
     8881 tgcccttcgc ctaaagcacc ttgaagtgct tgatgtccat catgcttccc gtccgcttcg
     8941 gctcgaagac cgccttgacc cgggcgccca ccttgacctt caccggatcg acgctgtcga
     9001 ggaaatgggt caggcaggtg tcggcgccgt ccagcttgat aaaggcatac ccgtggggca
     9061 ccggccgctg gaccccggta ttcggatcca cgaagccgaa gtgcacgatc gtaaagacgt
     9121 aaatctcccc ctcgtcggaa acgggaacga tctcgttcat gccgacgtag cattgaccgc
     9181 agacgggccg gggcggcaca tagacctttc cgcacttggg gcacttgatt ccgaccagtt
     9241 tcttgtggtc gcgcatctct ctgagaaagg tggagccata ctcgtccacc gtccagttga
     9301 acggctggct ggagtccccg gatgtcacca tcagaagctc ctgagcgtct tttttcgttt
     9361 ctgtcatcat atcctccctc atgactgctg agataggctg cacgcccgac cccgggctcg
     9421 cccgacggcg gccggatcga cgggcaaccg gatccatcac gcgtctcgtg aaaaactccc
     9481 catgttcaag cgggcttctt cctgcccaag agcatgatgt ccgcccagta gcaaccgccg
     9541 aatcccgagc agaaggccag attcaccttc cggttgacct ggcgcgcccc cgcccgcccc
     9601 atgacctgca gggccccctc gcccacccgg atcagggccg tcgccccgat ggcgttggaa
     9661 ctcatgaccc cgcccgaggg attgatcggc atttcgccgt ccatgtccgt cacgccgtcc
     9721 cagaggagcc ggggcccctc gccgtacccg cagaaaccaa tgtcctcgat ccacttgagg
     9781 ccggcgtagg agtacggcag gtaaagctcg cagacgtcga tctgcttgag cggctccttg
     9841 atgcccgcct tgcggaagac cgcctgcgag gcctcggcga tcgtcgtgtt gcgggccatg
     9901 tccacgtcgc cggcgtagac gaaattgtgg cgattggccg tggcttgcac ccaggccggg
     9961 atcggcgcga tcttttccgc cttcccctcg acggcgaaga tcacggccgc cgcgccgtcg
    10021 gtgcggggac acatgtcgct catccgcagg gggtaggcca gcagctggtt ctgctccgaa
    10081 ccgacgatca ggtccaccag ttgatccggg gggatgccct tcatccactc ctggtgcagg
    10141 tgggcgtagg ggttgttcag agcgtggagc cgttcgcgca cggccacccg cgcgccgtcc
    10201 ttctccgtgg ccccgtagtc gtgcatgtac ttgctcgcct cgatggccag cccggccacg
    10261 gcgccgccca ggttgggccg ctcccaaacc ggatggaagg ccgtgatgat cgccccggtc
    10321 gtgtcggact cggagttctt ctcccagccg atggccagta ccttgtcgaa catccccgaa
    10381 gcgacgtggt aataggcgca catggccacc gacgttcccg tcgtcccgcc ggtcgtcacc
    10441 ttcatgaccg gtttcatgac ggcaccggaa ccttccgccg accacatgtc cacatagttg
    10501 atcccttcga agtgatccat attgccgatc acaatggcgt cgatatcctt gatggtcagc
    10561 tccgcgtccg ccagggcaac cctcaccgcc tcattgatga gttccacccc gttgacatcc
    10621 ttgcgcgagg agcgctggta ggtctgacca tatccgacaa cggctacgcg tttccccata
    10681 tcttcctcct taagcaccga cgatgaatac gcaatgggcc tgtccgcagg ggccgttgat
    10741 cccctgggcg agcgccgttt tcgcgccctt gacctgccgc ttgtccgcct cgccgcggac
    10801 ctgcagggtc gcttcgatga gccggatcag tcccgccacg atgaccgggt ggccggagag
    10861 gacgcccccg gaaggattca cgggcaggac gccgcccagc gccgtggccc cttcccggat
    10921 gagctgtttt ccctcgccct ccggcgccag ccccaaaccc tcgtaccaca tcaattccat
    10981 gtacgagtag gcgtcgtaga gttccgccac gtccagctcc ttcaaaggat ccttgatctt
    11041 cgccatcttg taggccttct tcgcggcctc cccgagggaa cgggagcggg ccagatcgcg
    11101 atagccgagg ttgaaggcat cggtacaatg gccgacgccg cggatccaga cgggatcctt
    11161 cttgaagcgc ttgaggccgc gccggttcga gaggacaacc gcggccgccc catcgctgat
    11221 cggggagcag tcgagcagtt tgagcggctc ggcgatcgga cgggattcca gcacgtcctt
    11281 caaggtaagc ttcatcggca gctgggcgaa ggggttcttc atcgcctggc cgtggttctt
    11341 gaccgacacc cgcgcgaaat cgcgctccgt cgccccgctc atctgcatgt agatgcgagc
    11401 ctgaagggcc gccgccgcgg tcgcgtcgat cccgagcgag cgctggtaga tgggatcgaa
    11461 catggcgttg gtgatcaggt tcatcacccc ctcggagccc ttgatgtgcc ccaccacgag
    11521 ggccgtgtcg aaggacccgg acaaaatgcg catcactccg tagaaggccc cgaaggcgcc
    11581 gtcgccctcg accgtggaga tgtccttgtg ccacccgccc gccgcctcct gaacggccat
    11641 cgaggagatc gtccggccgt cccagaagtc gtttgagacg gtgacgacgt tgtcgatgtc
    11701 accgatctcc gcgccggcgt cagccagggc tttcgtcgcc gcttcgaata cgagctgttc
    11761 gaacgtttct tctctgcgcc gggcgcacac tgtctgtccg acgccgacga tagcaatgtt
    11821 ttccatagtt ttccccctcc gtgagtctat gccggagcaa agtgttggat gtcgaggatc
    11881 gagcctgccc gctggcgggc aaaaaccgcc ttcatgcgca tgccgagctt gatcttggcc
    11941 ggttcgacgc cgccgatcag gtgcagcagg gcgccgtcgg tgccgtcgag tttgacgaga
    12001 ccgtagatca gcgggcgctt gcggggctgc atgtcgtgct tgtagtgcac caccgtaaag
    12061 gactcgagtg tcccgacgtt cgccaccttg acccactgct ccgtagggac gaaacactct
    12121 ccacagttct tgatcgggta tacatagacg cgcttgcact tggggcactt gacaccccag
    12181 atctcctggt catccctgag ggcggtcaga aaccggcttc ccgtcgcccc gacactccac
    12241 gcatagg
//
LOCUS       DAOOLJ010000058        11792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_6059_length_11792_cov_17.450115, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000058 DAOOLJ010000000
VERSION     DAOOLJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6059_length_11792_cov_17.450115"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLW16_08395"
     CDS             complement(<1..473)
                     /locus_tag="PLW16_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HPB70965.1"
                     /translation="MKRLWFFAGCLLAGLIGMLGGPGPVDAAKSYETCAEINDDALRL
                     RCYDEAAGRSRPEQPDSPDLAATPPPEQRATIAASGGSALSRIWQLDDESRRRRFAIM
                     PHRQNYILPYAYNFTQDKATYEKANPGTEIQNAESKFQLSLKMKLWEDIFGTKMDL"
     gene            complement(821..2140)
                     /gene="der"
                     /locus_tag="PLW16_08400"
     CDS             complement(821..2140)
                     /gene="der"
                     /locus_tag="PLW16_08400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPB70966.1"
                     /translation="MKPLIAIIGRPNVGKSTLFNRLTDRKKAIVIDQPGATRDRNYAD
                     GRWRDRRFTVIDTGGFEPATEERILIQMREQTLLAIEEADVLLFLMDGRTGLAPADID
                     IARTLRGLDKPVFFVVNKIDGARHEDLAYEFYRLGVEEIHPVSAQHGLGVAELLDRVI
                     EHLPPAEAEAEDDPDEEPRRIAVIGRPNVGKSSLINRILGYERTIANPTPGTTRDPID
                     TPFRYNRRDYLFIDTAGIRRKSRISVTLEKYTIVQAMKAIDRSDITLLLVDAVEGVTE
                     QDVKIAGLAVEKGSAVILCVNKWDLVDKDTATAGAFVADVRDKLKFLDFAPVVFISAL
                     TGQRVRRILEIIEGVYDQYTRRIPTAELNRQARDFIAAHPPPRYRNRDNAVSYLTQVG
                     VKPPTFIFFVKEPKGMHFSYERYLTNQVRSAFGFDQVPVRIFFKRKS"
     gene            2457..5204
                     /locus_tag="PLW16_08405"
     CDS             2457..5204
                     /locus_tag="PLW16_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPB70967.1"
                     /translation="MNSKTFFHQTTPPRPPGQGRAFVAILAGFVALTLIWQAADLITD
                     WYWFQEVGYERVFLVTLTTQIKTALLFAAVFFALFFGNLSLARALSRKPFFEVREGTI
                     VLPPWKMGDRAIGGLILLASLLFSVFAALQGAVRWESVLRFLNATPFGTPDPLFKRDI
                     GFYVFQLPLLNDLYGWLMGVLIFTLLSTLLVYLLRRAFVFMPPRTVQVSPAARAHLVA
                     LVAVLFFLGVFGFWLGLSNLLFEKRGVVSGPGYTDVTTQVWVLKALMGVAALTGAALV
                     ASLFRRDWRIPAVAVLVFFVCIVVGRGMYPAFVQKFKVVPNEIVLEKPYLEWNIHYTR
                     LAYNLDNMEDRDFPAEENLTAADLKRNDLTIKNIRLWNHAPLLRTYSQIQEIRTYYKF
                     RDVDNDRYMVNGEYRQVMLSPRELSYDALPSRSWVNEHLVYTHGYGAVMGPVNRVTRE
                     GLPEFFIKDIPPVATTNVRVTRPEIYYDETSNEYVFVKTKRPEFDYPVGDQNVYARYE
                     GKGGVPLNFWRKVLYAMHFGSLTILLSDDIPAGSRVMYHRSIRERVSRVAPFARFDAD
                     PYLVITPRGRIVWFVDGYTLTDRIPYSEPIPRLGNYIRNSLKAVVDAYDGTVQLYIAD
                     GADPIVKTYARIFPGIFRPLSEMPADLQPHVRYPQGMLSIQARMYRTYHMQDPQVFYN
                     KEDMWEIPSQGAAGGDREMEPYYTIMKLPEGEKEEFILLLPFTPRNKDNLSAWMAARC
                     DAPHYGKVVVYNFPKQKLIYGPRQIEARIDQESEISKQISLWNQSGSQVIRGSLLAIP
                     IEKSILYVQSLYLAAEKGQLPELKRVIVAFGNAIAMEETLELSLRRIFGGGATVRERA
                     GGDVVPAAAATAESGDRRMIREALDHYRKAQDHLRQGNWGGYGDELKRMADLLERLEK
                     R"
     gene            5458..6657
                     /locus_tag="PLW16_08410"
     CDS             5458..6657
                     /locus_tag="PLW16_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB70968.1"
                     /translation="MKTIYFEVSIPKIILTKLLAKPFPRAFYSSVSPVRYREIPDRDL
                     PGRNWVRVKNRQAGICGADLSLFFVKADPRITIAALPGVPRAFMGHELVGAVMETGAG
                     VADLAVGDRVVLQRYLPCCSIKEIEPPCDPCREGNYTLCENFSEGEMPENLGAGFGDH
                     FIAHRSQLIKIPDSISDDTAVMIEPTSVSLHAVMKRPPRKGERLLVLGAGVIGLNVIR
                     FARLLQPECRIYVLEKMAFKQDLARKMGADVILEGDPYESVAVHTGARLYRGPLGNTV
                     LMGGFDLIYDCVGQSSTIHDALRWLKSRGDYVMIGNQLSPVKFDQTPVWNQEINLTGV
                     NAHGLETYQGEKISSFDLTLRMITQGVVNLDGFITHRFPLADYRKAFRRIQSGGEKII
                     KAVLVCE"
     gene            6719..7558
                     /locus_tag="PLW16_08415"
     CDS             6719..7558
                     /locus_tag="PLW16_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB70969.1"
                     /translation="MPITDQELRELRGHAKQLRKDIIDVTFWAGGAHIGGALSLVEIM
                     ILLYYKYLHIDPDHPDMPERDRFILSKGHAGVGFAPMLARRGYFDFELLKDFNKFNSP
                     FGMHLDGNKVKGADVSTGSLGHGLSMAVGMALGARLKGRSWRTYCVLGDGECNEGSVW
                     EAAMAAAHYKTTNLITFVDRNKYSVDGPTETVMGIEPLGDKWRAFGFIVKEVDGHSYA
                     ALSQAIEEAQAETKAPVVIIAHTIKGKGVDFMEDDVRWHYGGLDSDKIVEAKRYIETA
                     VIG"
     gene            7604..8614
                     /locus_tag="PLW16_08420"
     CDS             7604..8614
                     /locus_tag="PLW16_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPB70970.1"
                     /translation="MAVGLTWNVYDANTMTQAEIYGKVLCRLGEENTDIVGLSADLAK
                     STKIGEFAAMFPDRFFNAGVAEQNMFGMAAGLAKAGLIPFVSTMAVFTTMRAAEQVRT
                     DICYQNLNVKIIATHGGISFGQAGTTHQCTEDIAITRAMANMTVIVPADGIECANAVR
                     ACLDINGPVYIRIGRGFEPNCYTTDAYGFEIGKAVEMNPGTDITLICCGITVLQALEA
                     ARDLEKSAGISARVINMHTLKPLDGEIIRKAVAETRRIITFEDHNIYGGLGSAVAEVI
                     AEYGKSCAFARVGLPDCFSLVGYPEDLYSHYKLDADGIIEKVKEVMGKDFEDDDSWED
                     GF"
     gene            8616..9395
                     /locus_tag="PLW16_08425"
     CDS             8616..9395
                     /locus_tag="PLW16_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70971.1"
                     /translation="MASHEERLAAGLFLHALFPLLKVVMNENAGVRKKFAGVNASVQF
                     QARDGEGDMGAYLKFADGRLDVVEGTCPKPDIAFLFASVKRMNAMLGGKPALFRIRGF
                     YRVPLLVKVLALLMNLTLLMPTARPADPEKKRLKVKLTLYMMAASLSQFNKLGNPDIR
                     KWTSMQPERIYQFSVAGREDMAAYLRVKAGKTQAGPGLYAKRRPFVHMKFRSVDEALP
                     IILNEVNMVMAVEKGYLALEGSPEYARDIGNFMMQIQSLIM"
     gene            9447..10397
                     /locus_tag="PLW16_08430"
     CDS             9447..10397
                     /locus_tag="PLW16_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB70972.1"
                     /translation="MAESDRILGMTGSFGYIGKRLIRRLCDDTAFKKIICTDILPPPE
                     APPEKFEVHRCDIRDRERLSAVLREGAVDTLIHLAFIANPTRDPGFEYDVDVNGTRNV
                     LHACKSLKIRKLVVASSDCAYGFFEGTPDYLSESAPVRSTPGFPYSENKAAIEQLLAD
                     FIADTPECAVVILRPCIVMGPNANNTTSKSMKDPVIIGVRGYDPIMQFIHEDDAAEAF
                     YLAAVRDVQGVFNLAADGGLRYSELARVLGKPMISLPVWLIYPLVELLYRLRVVPFGR
                     AQLDYIRYPLSMDVEKIGRVMGFKPKYTTRETLRTLLPDP"
     gene            10492..10680
                     /locus_tag="PLW16_08435"
     CDS             10492..10680
                     /locus_tag="PLW16_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70973.1"
                     /translation="MKRNVRPMRTEDVPSGWSDRRRIREALDHYRKAQDHLRQGNWGG
                     YGDELKRMADLLERLEKR"
     gene            complement(10637..11143)
                     /locus_tag="PLW16_08440"
     CDS             complement(10637..11143)
                     /locus_tag="PLW16_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPB70974.1"
                     /translation="MSEKAFQEYYPDILSHCYGCGRLNEHGYRIRSTWDGDESVCTFQ
                     PGPYHISMPGFVYGGLLASLVDCHGTGTAAAAAYRAEGRAMDTEPPLRYVTASLHVDY
                     LRPTPLGPPLELRGRIKEIKGRKVVVAVTVAANGQVCARGEVVAVQVPEDMLPTASPG
                     ARGDRPSA"
     gene            complement(11237..>11792)
                     /locus_tag="PLW16_08445"
     CDS             complement(11237..>11792)
                     /locus_tag="PLW16_08445"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPB70975.1"
                     /translation="KEGGAMKTLEEMTREEIVDLLNRSWMTHDGMWFYHCFQACGIDT
                     ANRVNKAAIGSLAPLEMARMKKALGFSGDRIRSFAEFREFFTAAARLCIPPFMNGTMD
                     TSRENVLHWEFAPKNCFAYKGMMRIGALDGYECGVIYRLACWFDALGLTYRATPEITG
                     CRMRTGDTCGGDFVFDFGPAPSAA"
BASE COUNT         2158 a         3695 c         3725 g         2214 t
ORIGIN      
        1 aggtccattt ttgttccgaa gatatcttcc cagagcttca tcttcaggct gagttggaac
       61 ttggattcgg cgttctggat ctccgttccc gggttggctt tctcataagt cgccttgtct
      121 tgggtaaagt tgtatgcgta agggaggatg tagttctgtc ggtgtggcat gatggcgaac
      181 ctgcgccggc gactctcgtc gtcgagttgc cagatgcggg agagggcgga ccccccggac
      241 gctgcaatcg tcgctcgttg ctcagggggg ggtgtcgctg ccaaatccgg cgagtccggt
      301 tgttctggcc tgctgcggcc cgctgcttcg tcgtaacagc gaagccgcaa ggcatcgtcg
      361 ttgatttccg cgcacgtttc atagctcttg gcggcatcga ccggccccgg gccgcccagc
      421 attccgatga gaccggcgag caggcagccc gcaaaaaacc ataatcgctt catacccccc
      481 tccctccttg catgacctcc gtgtatgagc atatgccagt cctgcgacgg gatttcaagg
      541 tcaacccgca tcttttctta caaacagccg actcagggtc atgccggaac cggctccgga
      601 cgacgggggt gctcatggat tttcacttaa aaggacgtgg gttcgtcaag attaccggac
      661 gcgctcattc atgaagagtg cattgaacgg atgaacgacc gggcagggtg ccgcccgcct
      721 gatgccgcgg ctcggcgagg atcggaacag cgggaagatg cgccgtccct ccggagcctg
      781 ggaaatgccg tgggcgcgga tcgatctccg ggggcgggga tcaggacttg cgcttgaaga
      841 agatccgcac ggggacctgg tcgaagccga aggcgctccg cacctggttc gtcaggtagc
      901 gctcgtagga gaagtgcatc cccttcggct ccttgacgaa gaagatgaag gtcgggggct
      961 tgacgcccac ctgcgtcagg tagctgacgg cgttgtcccg gttccggtag cgggggggcg
     1021 ggtgggcggc gatgaaatcc cgcgcctgcc ggttcagctc ggccgtcggg atccgtcgcg
     1081 tgtactggtc gtacaccccc tcgatgattt ccaggatcct gcggacccgc tggccggtca
     1141 gggccgagat gaacacgacg ggcgcgaagt cgaggaactt gagcttgtcc cggacgtcgg
     1201 ccacgaaggc gccggccgtg gcggtgtcct tgtccacgag gtcccatttg ttgacgcaca
     1261 ggatgacggc gcttcccttc tccacggcga gccccgcgat cttgacgtcc tgctccgtga
     1321 cgccttccac ggcgtcgacg aggaggaggg tgatgtcgct ccggtcgatg gccttcatgg
     1381 cctgcacgat ggtgtatttc tccagggtca cgctgatccg gctcttacgg cggatgccgg
     1441 ccgtgtcgat gaagaggtaa tcgcggcggt tgtagcggaa cggggtgtcg atgggatcgc
     1501 gggtcgtgcc cggggtgggg ttggcgatgg tccgctcgta ccccaggatg cggttgatga
     1561 gggacgattt gcccacgttg ggccggccga tgacggcgat gcgccggggt tcttcgtcgg
     1621 ggtcgtcctc cgcctccgcc tccgcgggcg ggagatgctc gatgacgcgg tccagcaact
     1681 cggccacccc gaggccgtgc tgcgccgaga cgggatggat ttcctcgacc cccaggcggt
     1741 agaattcgta ggccaggtct tcgtgccggg cgccgtcgat cttgttgacg acgaagaaga
     1801 cgggcttgtc cagcccccgg agcgtccggg cgatgtcgat gtccgcgggg gcgagccccg
     1861 ttcggccgtc catgaggaac aggaggacat ccgcctcctc gatggcaagg agcgtctgct
     1921 cccgcatctg gatgaggatg cgctcctccg tcgccggctc gaaccccccc gtgtcgatga
     1981 ccgtgaagcg ccggtcccgc cagcggccgt cggcgtagtt gcggtcccgg gtggcccccg
     2041 gctgatcgat gacgatggcc tttttccggt ccgtcaggcg gttgaacagg gtcgatttcc
     2101 ccacgttcgg ccggccgatg atggcgatga gcggtttcaa gcggcgtccc ttttctctga
     2161 tggctggtcc cgttgttccg ggaatcggat ccctccgcgc cggacctctt gcgaccccgt
     2221 gtctcctccg tgcgctcagg ccgcgggggg tccgaaaaga ctcccacgca cgcctgcggg
     2281 atccgacttc cggatcgggc acacatttct tgtcacagat cgcccgaaag atcaatcctt
     2341 ccgacgccgc gccggcgccc gcgagtggcg cttccggcgg agaggggacc aattgtcttg
     2401 acaccgcttc gctatttccg atagaacaca tggaaagaaa gacccaagga gggctcatga
     2461 attcaaaaac atttttccac cagacgacgc ctccgcgccc gccggggcag ggccgcgcgt
     2521 ttgtggcgat cctcgccgga ttcgtggcgc tcacgctcat ctggcaggcg gccgacctga
     2581 tcacggactg gtactggttc caggaggtcg gctacgagcg ggtgtttctc gtcaccctga
     2641 cgacgcagat caagacggcc ctcctcttcg cggcggtctt cttcgccctc ttcttcggaa
     2701 acctctccct ggcgcgcgcc ctgtcccgaa agcccttctt cgaggtgcgg gagggaacga
     2761 tcgtcctgcc cccgtggaaa atgggcgacc gggccatcgg gggcctgatc ctgttggcct
     2821 ccttgctgtt ttccgtcttc gccgcgcttc agggcgccgt ccgctgggaa agcgtgctgc
     2881 gcttcctcaa cgccaccccg ttcgggaccc cggatcccct gttcaagcgg gacatcggct
     2941 tttacgtctt ccagctcccc ctgctcaacg acctctatgg ctggctcatg ggcgttctga
     3001 tcttcaccct tctgtccacg ctcctcgtct acctcctgcg gcgcgccttc gtgttcatgc
     3061 cgccgaggac cgttcaggtc tcccccgccg cgcgggcaca cctggtggcg ctggtggcgg
     3121 tgctcttttt cctgggcgtc ttcggattct ggctgggcct cagcaacctc ctgttcgaaa
     3181 agcggggcgt cgtctccggg cccggctaca cggacgtcac gacccaggtg tgggtcctca
     3241 aggcgctgat gggggtcgcc gcgctgacgg gggcggccct ggtcgcctcc ctgttccgcc
     3301 gcgactggcg catccccgcg gtggccgttc tcgtgttttt cgtctgcatc gtcgtcggcc
     3361 gggggatgta ccccgccttc gtgcagaagt tcaaggtcgt gcccaacgag attgtcctgg
     3421 agaagcccta tctggagtgg aacatccact atacgcgcct ggcttacaac ctcgacaaca
     3481 tggaggatcg agactttccg gcggaggaga atctcacggc ggccgatctc aagcgcaacg
     3541 acctgacgat caagaacatc cggctgtgga accacgcacc cctgctcagg acctacagcc
     3601 agattcagga gatccgcacc tactacaaat tcagggatgt ggacaacgac cgctatatgg
     3661 tcaacgggga ataccggcag gtcatgctct ccccgcgcga gctgtcgtac gatgcgctgc
     3721 cgtcgcgctc gtgggtcaac gagcaccttg tgtacacgca cggctacggc gccgtgatgg
     3781 gtcccgtgaa ccgcgtcacc cgcgagggcc tcccggagtt cttcatcaag gacatccccc
     3841 ccgtcgccac gacgaacgtc cgcgtcaccc gcccggaaat ctactatgac gagacgtcca
     3901 acgaatacgt cttcgtcaag accaaacggc ccgagttcga ctatcccgtg ggcgaccaga
     3961 acgtctatgc ccgttacgag gggaagggcg gcgtgccgct caatttctgg cgcaaggtcc
     4021 tgtacgccat gcacttcggt tctttgacga tcctcctgtc ggacgacatt cccgccggca
     4081 gccgcgtgat gtaccatcgc agcatccgcg aacgcgtgag ccgcgtcgcc cccttcgccc
     4141 gattcgatgc cgacccctat ctcgtcatca cgccgcgggg gcgtatcgtc tggttcgtcg
     4201 atggctacac cctgacggac cggatcccct attccgagcc gatcccccgc ctggggaact
     4261 acatccgcaa ctccctgaag gcggtcgtcg atgcttatga cgggaccgtg cagctctaca
     4321 tcgccgacgg tgccgatccg atcgtgaaaa cctacgcccg gatttttccg gggatcttcc
     4381 gcccgctctc ggagatgccg gcggatctcc agcctcacgt gcgctatcct cagggaatgc
     4441 tctccatcca ggcccggatg taccgcacct accacatgca ggacccccag gtgttctaca
     4501 acaaggagga catgtgggag atcccgtcac agggcgccgc ggggggtgat cgggagatgg
     4561 agccctatta cacgatcatg aagcttcccg agggcgagaa ggaggaattc atcctcctgc
     4621 tgccgttcac cccgcggaac aaggacaacc tgtccgcctg gatggcggcg cgctgcgacg
     4681 ccccccacta cgggaaggtg gtcgtctaca acttccccaa gcagaagctc atctacgggc
     4741 cgcggcagat cgaggcccgc atcgaccagg agtcggagat ctccaaacag atctcgctgt
     4801 ggaaccagag tggctcccag gtgatccgcg ggagcctgct ggccattccc atcgagaagt
     4861 ccatcctgta cgtccagtcc ctgtacctgg ccgcggaaaa ggggcagttg ccggagctca
     4921 agcgggtcat cgtggccttc ggcaacgcca tcgccatgga ggagaccctg gagctgtcgc
     4981 tgcggaggat cttcgggggt ggcgcgaccg tccgggaacg ggcaggaggg gacgtggttc
     5041 ccgcggcggc tgcaacggcc gaaagcggcg accgccggat gatccgggag gccctcgacc
     5101 actaccgcaa ggcccaggac catctccgtc agggaaactg gggcgggtac ggcgatgaac
     5161 tcaagcggat ggccgatctc ctcgagcgcc tggagaagcg gtaggcagca tgtcctcggg
     5221 cacctgcacg gccaccacct cgccgcgggc gcacacctgc ccgtttgcgg cgaccgttac
     5281 ggccacgacg accttccgcc ccttgatttc cttgatccgt ccgcccgtga ttcccggcag
     5341 ggacaggggg caatattgtc ttgacttccc cgggcgttgc cgcttacact ctcgccgcca
     5401 ttagtcatgc ggcatcgaaa atccccatca gcatcttcac attgaccagg gagcgttatg
     5461 aaaacgatct atttcgaggt cagtattccg aagatcatcc tcaccaagct gttggccaag
     5521 ccttttcccc gcgcctttta ttcttccgtt tcgcccgtgc gatacaggga aatccccgac
     5581 cgggatttgc cggggagaaa ctgggtacgg gtcaagaaca ggcaggccgg aatctgcggc
     5641 gccgatctgt cgctcttttt cgtgaaggcg gatccccgga ttacgatcgc cgccctgccg
     5701 ggcgtgccgc gggcgtttat ggggcacgaa ctggtgggcg ccgtcatgga aaccggggcc
     5761 ggcgtggccg acctcgcggt cggcgaccgg gtggtgctgc agcgctatct tccctgctgc
     5821 tcgatcaagg agatcgagcc cccctgcgac ccctgccggg aaggaaacta caccctctgt
     5881 gaaaattttt ccgaaggcga aatgcccgag aatctgggtg caggattcgg ggaccacttc
     5941 atcgcgcatc ggtcccagtt gatcaagatc ccggattcga tctccgacga taccgcggtg
     6001 atgatcgagc cgacctccgt ctcccttcac gccgtcatga agcgtccgcc ccggaagggc
     6061 gagcgccttc tggttctggg cgccggcgtc atcgggctga acgtcatccg cttcgcacgc
     6121 ctcctccagc ccgagtgcag gatttatgtc ctcgaaaaaa tggccttcaa acaggatctg
     6181 gcccgaaaga tgggcgccga tgtcatcctg gaaggcgatc cgtacgagtc cgtggccgtc
     6241 cataccggcg cccgcctcta ccgcggcccg ctggggaaca ccgtcctgat gggcggcttc
     6301 gatctcattt acgactgtgt cggacaatcg tccacaatcc atgacgcgct ccggtggctg
     6361 aagtcccgcg gcgactatgt catgatcgga aaccagcttt ccccggtaaa attcgaccag
     6421 acgccggtct ggaaccagga gatcaacctg accggcgtca acgcgcatgg attggagacg
     6481 tatcaaggcg aaaaaatcag cagcttcgat ctgaccctcc gcatgatcac ccagggggtc
     6541 gtgaatctgg atggatttat tacccaccgc tttccgcttg cggattatcg gaaggccttc
     6601 aggcgcattc aaagcggggg agagaagatc atcaaggccg tcctggtgtg cgaatagggc
     6661 ggatcatcgg tccggatgcg gccgaatagc catccacacc cacgaaggag cttgtgaaat
     6721 gccgattacc gatcaggaat tgagggaact gcgcgggcat gcgaaacagt tgcggaagga
     6781 cattatcgac gtcaccttct gggccggcgg cgcgcacatc ggcggcgccc tgagcctggt
     6841 ggagatcatg atcctgctct attacaagta tctccacatc gatcccgacc atccggacat
     6901 gcccgaacgc gacagattca tcctgagcaa gggccatgcc ggggtgggat ttgccccgat
     6961 gctcgcccgc cggggatatt tcgatttcga actcctgaag gacttcaaca agttcaattc
     7021 gcccttcggc atgcatctgg acgggaacaa ggtcaagggc gcggatgtct ccaccggttc
     7081 cctcggacac gggctgtcca tggcggtcgg catggcgctg ggggcgaggc tcaagggccg
     7141 ttcgtggcgg acctattgcg tcctgggcga cggcgagtgc aatgaggggt ccgtatggga
     7201 ggcggccatg gcggcggccc actacaagac gacgaacctg atcacgttcg tggaccggaa
     7261 caaatattcg gtggacggtc cgacggagac cgtgatgggc atcgaaccct taggcgataa
     7321 atggcgggcc ttcggcttca tcgtcaagga ggtggacggc cacagttatg ccgcgctgtc
     7381 gcaggccatc gaggaggcgc aggcggaaac gaaggcgccg gtggtgatca tcgcgcacac
     7441 gatcaagggc aagggggtcg atttcatgga ggatgacgtt cgctggcatt acggcgggct
     7501 cgactccgat aaaatcgtcg aagccaagcg gtacatcgaa acggccgtga tcgggtgatc
     7561 atggccggct catggaacgg acgcactcac ggggagggtt atcatggcgg tcggattgac
     7621 atggaacgtg tatgacgcaa acaccatgac ccaggcggag atttacggca aggtcctctg
     7681 ccggctcgga gaagaaaaca cggacattgt cgggctgtcg gcggatctgg cgaaatcgac
     7741 caagatcggc gaattcgccg caatgtttcc cgatcgtttc ttcaatgccg gcgtcgcgga
     7801 acagaacatg ttcggtatgg ccgcgggcct cgccaaggcc ggtctgattc ccttcgtgtc
     7861 gacgatggcc gtgttcacca cgatgcgggc ggccgagcag gtccgcaccg acatctgcta
     7921 tcagaacctc aacgtcaaga tcatcgcgac ccacggcggc atttccttcg ggcaggccgg
     7981 gaccacccat cagtgcacgg aggacatcgc catcacccgc gcgatggcca acatgacggt
     8041 gatcgttccg gccgacggca tcgaatgcgc caatgccgtc cgggcctgtc tcgatatcaa
     8101 cgggcccgtt tacatccgca tcgggcgcgg cttcgagccc aactgctaca cgaccgatgc
     8161 gtatggattc gaaatcggga aggccgtgga aatgaatccc ggcaccgaca tcacgctgat
     8221 ctgttgcggc atcaccgtgc tccaggcgct ggaggcggcg cgggacctgg aaaaaagcgc
     8281 cggcatctcc gcccgcgtca tcaacatgca caccttgaag cccctggacg gcgagatcat
     8341 ccggaaggcc gtcgccgaaa cgcgccggat catcacgttc gaggaccaca atatctatgg
     8401 cgggctggga agcgcggtgg cggaagtcat cgccgagtac ggcaagtcct gcgccttcgc
     8461 ccgcgtcgga ctcccggact gcttctccct ggtgggttac cccgaggatc tgtacagcca
     8521 ttacaaactc gatgcggacg gcatcatcga aaaggtgaaa gaggtgatgg gcaaagactt
     8581 cgaggatgac gacagctggg aggacggatt ctgacatggc atctcacgag gaaagactgg
     8641 cggcgggcct gtttttgcat gcgcttttcc ccctgttgaa agtcgtcatg aacgaaaacg
     8701 ccggcgttcg gaagaagttc gccggcgtca acgccagcgt gcaattccag gcgcgggacg
     8761 gcgagggcga catgggcgcg tacctgaaat ttgcggacgg ccgtctcgac gtcgtcgagg
     8821 ggacctgccc caagccggac atcgccttcc tgttcgccag cgtcaagaga atgaacgcga
     8881 tgctgggcgg caagccggca ttgttcagga tcagggggtt ttaccgggtc cctctgctgg
     8941 tgaaggtgct ggcgctcctg atgaacctga cgctcctgat gccgacggcc cggccggccg
     9001 accccgagaa gaaacggctg aaggtcaagc tgacgcttta catgatggcc gcatcgctca
     9061 gccagttcaa caagctgggc aatccggaca tccggaaatg gacgtccatg cagcccgagc
     9121 ggatctacca gttctcggtc gcgggacgcg aagacatggc cgcttatctg cgcgtcaagg
     9181 ccgggaagac gcaggccgga cccggcctct acgcgaagcg ccgccccttc gtgcacatga
     9241 agttccggag cgtcgacgag gcgctgccca tcatcctcaa tgaggtcaac atggtgatgg
     9301 cggttgagaa gggctatctc gcgctggaag ggtctccgga atacgcgcgg gatatcggca
     9361 atttcatgat gcagatccag tctctcatca tgtaaccggc gaggcggcgc cggacggacc
     9421 cgccggaaac cgacaggaga atcgcgatgg cggaatcgga cagaattctg ggcatgaccg
     9481 gatccttcgg gtacatcggc aagcggctga tcaggcgtct ttgcgatgac acggctttca
     9541 agaaaatcat ctgcacggac atcctgccgc cgccggaggc cccgccggaa aaattcgagg
     9601 ttcatcgctg cgacatccgg gaccgggagc ggctgtctgc cgtccttcgg gagggagccg
     9661 tcgacaccct gattcatctc gcctttatcg ccaacccgac ccgggatccg ggattcgagt
     9721 acgacgtcga cgtcaacggc acccgaaacg tcctgcacgc ctgcaagagc ctgaagatca
     9781 ggaaactggt cgtggccagc tcggactgcg cctacggatt cttcgagggg acgcccgatt
     9841 acctctcgga gagcgcgccg gtgaggtcca cgcccggttt cccctactcg gaaaacaagg
     9901 cggccataga acaactgctg gccgatttca tcgccgacac gccggaatgc gccgtcgtga
     9961 tcctgcggcc ctgtatcgtg atggggccga atgccaacaa cacgacctcc aaatccatga
    10021 aggacccggt catcatcggc gtgcgcggat acgatcccat catgcaattc atccatgagg
    10081 acgacgccgc ggaggccttt tacctcgcgg ccgtgcgaga cgttcagggc gtcttcaacc
    10141 ttgcggcgga cggggggctt cgctactcgg aactggccag ggtgctggga aaaccgatga
    10201 tctccctgcc ggtttggctg atctatccgc tggtggaact gctctatcgc ctccgcgtcg
    10261 tgcctttcgg gagggcgcag ttggattata tccgttaccc gctgtcgatg gatgtggaga
    10321 agatcggccg ggtgatggga ttcaaaccga aatacacgac ccgggagacc ctccggacat
    10381 tgctgcccga tccttgagtc ctggacggtt tcacgcagcc aggatcccct ttgcgcgggc
    10441 ggaccgtgcc gtttaagcca acctgaacgg tcacggtcgg ccggggaggg ggtgaagcgc
    10501 aatgttcgcc cgatgcgcac ggaggacgta ccctccgggt ggagcgaccg ccggaggatc
    10561 cgggaggccc tcgaccacta ccgcaaggcc caggaccatc tccgtcaggg aaactggggc
    10621 gggtacggcg atgaactcaa gcggatggcc gatctcctcg agcgcctgga gaagcggtag
    10681 gcagcatgtc ctcgggcacc tgcacggcca ccacctcgcc gcgggcgcac acctgcccgt
    10741 ttgcggcgac cgttacggcc acgacgacct tccgcccctt gatttccttg atccgtccgc
    10801 ggagttcgag tggggggccc aggggcgtcg gccgcaggta gtccacgtgc agggaggcgg
    10861 tgacgtaacg caggggcggc tccgtgtcca tggcgcgtcc ttcggcgcgg tacgccgccg
    10921 cggcggccgt tcccgtgccg tggcagtcga ccagcgaggc cagaagaccg ccgtagacga
    10981 agccgggcat ggaaatatgg tagggcccgg gctggaaggt gcagacggac tcgtcaccgt
    11041 cccaggtgct ccggatacgg taaccgtgtt cgttcagccg gccgcagccg taacagtggc
    11101 tcaggatgtc ggggtagtat tcctggaagg ccttttcact catcgtcgaa gtgctcctct
    11161 cgaaaggggg taagcgtccg cccgggcctc ggccggcggg aaggcggggc gactatgcca
    11221 caaacggggc gatcgatcaa gccgcggacg gggccggtcc gaaatcgaac acgaagtcgc
    11281 ccccgcacgt gtcgccggtc cgcatccggc agcccgtgat ttccggggtg gcccggtacg
    11341 tcaggccgag ggcgtcgaac cagcaggcga gccggtagat cacgccgcat tcgtagccgt
    11401 cgagggcgcc gatccgcatc atccccttgt aggcgaagca gtttttgggt gcaaattccc
    11461 agtgcaggac gttctcgcgc gaggtgtcca tcgtcccgtt catgaagggc gggatgcaca
    11521 gccgcgcggc cgccgtgaag aactcccgga attccgcgaa gctccggatc cggtccccgc
    11581 tgaaccccag ggccttcttc atccgcgcca tctccagggg cgcgagcgac ccgatcgccg
    11641 ccttgttcac gcggttggcc gtgtcgatgc cgcacgcctg gaaacagtgg tagaaccaca
    11701 ttccgtcgtg ggtcatccag gaacggttca agaggtcaac gatctcctcc cgggtcatct
    11761 cctccagcgt cttcatggcg cctccctcct tt
//
LOCUS       DAOOLJ010000059        11723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_6114_length_11723_cov_16.448320, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000059 DAOOLJ010000000
VERSION     DAOOLJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_11723_cov_16.448320"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(594..2165)
                     /locus_tag="PLW16_08450"
     CDS             complement(594..2165)
                     /locus_tag="PLW16_08450"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPB70976.1"
                     /translation="MEKELKKSATREKIEAFERKRDALKEMGGAGPIAKQHEAGKLTA
                     RERLAMLFDPGTFQEVQLFVKHRSSLFGLGDKEIPADGVVTGFGHVNGRTVFAAAQDF
                     TSAGGSLGEMHAKKIWKVMDMALTARKPFVSLNDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALTDWVFMVKDRSYMYITGPDVIKAVIGEEVTHN
                     DLGGAVAHATKSGVCHFATESDEDCLAKVKTLLSYLPDSCHSPLPVRPCTDVPDRECP
                     ELDTIIPDKSSRGYDMKKVITAVADNGEVFETHELWARNITVAFIRIMGRPVGVIANN
                     SRFNAGVLDTHASDKASRFIRFCDAFNIPLLTFADVPGYMPGTQQEWSGIISHGAKLL
                     HAYSEATVPKITVVTRKDYGGAYIGMCSKQLGADYVMAWPSAEIAVMGAEGACNIVYR
                     REIGAAEDPAARRAELVAAYETQFNNPYFAASLGIIEEIIAPRDTRKRVVALFETLEN
                     KQESRPDKKHNNIPL"
     gene            2384..3571
                     /locus_tag="PLW16_08455"
     CDS             2384..3571
                     /locus_tag="PLW16_08455"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB70977.1"
                     /translation="MKYIDEIDIKGKRVLFRFDFNVPLDSSQNITDDTRIRAVLPTIN
                     YALDEGARIIMMAHLGRPKGKLMPEMSLAPVARRLSRLLGKQIPLAGDCLGPEVDRMV
                     GELQPGCLILLENLRFHPEETANDDAFAKRLAAYADVYIDDAFGNAHRSHASNVGITR
                     HVPVCGAGFLIKKELNYFRRALEKPARPFVAIIGGSKVSGKLEAMVNLIKKVDKMIIG
                     GGMAFTFLKAQGYEVGKSIVEDDLIPTAQDVLQTAREHGVKFYLPIDCVIAEAMTAEA
                     ETKIVPVQEMNAHWMGLDIGPATITLFTEALGNAKTVIWNGPMGVFEIDAFSRGTYAL
                     AHSVGNSYALTIVGGGDTDVAIHRAGESDKIGYISTGGGASLELLEGKVLPGIEALER
                     CGS"
     gene            3772..4527
                     /gene="truA"
                     /locus_tag="PLW16_08460"
     CDS             3772..4527
                     /gene="truA"
                     /locus_tag="PLW16_08460"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPB70978.1"
                     /translation="MDGMRTLKIVIAYDGARYHGWQRQLNGSSIQQVLEEAVGRITGR
                     PATVVGSGRTDAGVHALAQVAHFRTESGLAEANLLKGINSVLPKDIAITDLREVPEGF
                     HARFDAASKVYFYRIVNRSVRDVFEHGRAWFIHRPLAVEQMRQALPHFLGTHDFTSFC
                     TVHCESRDRIRTVLDAALTGEAGDIIETSIEADGFLRYMVRTIMGVLVDVGLGKHPPE
                     AVGEILQARDRCRAGMTAPARGLFLKEVRYGHV"
     gene            4524..5405
                     /gene="rfbD"
                     /locus_tag="PLW16_08465"
     CDS             4524..5405
                     /gene="rfbD"
                     /locus_tag="PLW16_08465"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPB70979.1"
                     /translation="MKILIIGHKGMLGSDLVLRLAASGHVVSGKDVGDVDITSPASCR
                     AAIAECDPEIVINAAAYTDVDACEANPDLCFAVNAEGVRHVAMACREYRVKLVHFSTD
                     YIFDGTKGQPYVEEDPGRPLNVYGASKLAGERYLIDLAPDFLLIRTAWLYGRHGKNFV
                     RTILAAARRRPTLDVVDDQVGSPTYTVDLAAAVERLIFEDRHGVFHVTNRGQASWFDF
                     AKTILAHAGLTDVAVRPLASTRLGRPARRPSYSVMSNRKFLQTTGRTMRFWQIALQDY
                     LSSVSILEQWSPSAEKP"
     gene            5521..6642
                     /locus_tag="PLW16_08470"
     CDS             5521..6642
                     /locus_tag="PLW16_08470"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HPB70980.1"
                     /translation="MMRRCILFVTLVGLMFGGVSLLYAQNRVVADGMAVVNNNRVDIA
                     RDRAVDNAQRNAVERVVGVMISSSTEVENFQVKMDRILSESQGFINSRKIVSERRDGD
                     MYEVTIEADVGMGKLKDRLAALNLIMARKEKPRLMLLFSGRAQKDAVAESAMSRYFLS
                     QGFKLVDADTYKKGTERENLQGLALNRRETANFGHRYGAEVLIVGDVEASSSSSRFGE
                     IEMTTNKVIVSTKIINVDTGEVLATDSETRSCPGMRDDIKQITVDAADSLAKKMTDEI
                     MERWSSELTNAVTVKLIVTGFNAYQDLLQFKEQIASDVRGFREIYQRAYAHGQAELDL
                     EIKGNTQGVADDLAAMTFNQRKLRITEITQNRIVAKLVP"
     gene            6720..8711
                     /locus_tag="PLW16_08475"
     CDS             6720..8711
                     /locus_tag="PLW16_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB70981.1"
                     /translation="MKRAMRTRMLSGLFVLLLLAGCAVSESFKVGQDLSGSQRWDEAI
                     IYFQKAVSEAPDNQEYKDALENAKREAAKVHLARARQLLAEASPEVTVPALERVLKEA
                     DTAVALDPQNKTGVAFKQEVGGEMEALQGKLKTLYGQAELDMQKEDWPAALTKLKQVN
                     QLFPNYEDTGNRLARSEQEAVKSLYQQGIALGKQEEWKLAAQVFKAAVEINPNYYDLS
                     ALAAQAQSRDNVQYYLTEADKAAGVQNWDRAITLYEKALEYQPENQELVGKMDGMKAK
                     VGQLYFDEAVKFMNQGKYYPAMKRVERIRTYAPELQDDPVFKEFLWNFCKQLMDRADK
                     YAQKELWGNALVWLEKVQAINPSYPELFQKVLDTKDRINKRIKKSIAVFDFSSPSNDK
                     DAGKIAANKLITFLHKNGSGDLRIIERENLQSILREMQLGQTGLVDVKSVQSVGKMRG
                     IDTFIMGDVLHFSARTTDMPSLGQVKVLVNEEDVRNPEFSDWLMMNPKPNADDLKHAP
                     PRTVKKRDYQFISYKQGSSKVTAIIEISYKLVDTLTGENIFTNTISGRLIKEDKYQDG
                     VAIANIPHDPLEIPTEVEVLDELTNEKISEMGQSVLKHYQSLEVEYFNHGQQQQKRRN
                     IDQAVERYTDAIYDEKLKGISTPISQKSMEFIVKLVQDK"
     gene            8772..11066
                     /locus_tag="PLW16_08480"
     CDS             8772..11066
                     /locus_tag="PLW16_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70982.1"
                     /translation="MKNRRFVSFFPLLWTIVFSLPVLAAAQPPTATRVSVFNFGTVNI
                     EASGYGTTVTNMLTTSLQSEASLNLMDRKELEAFLSLNDLQQNDLFENVIHIGTRLGL
                     NVIVVGNVQKRGTIIAIHCKVVHIEQKRVIFQTHVRALGDAGLLGEVRKLASMIVAAI
                     AENASRSLEDKPSMGGPVNVQKRSGNMRVSLNWEDPPDAPAAGYELFRSSTETGPFAK
                     IGQTTRPEYLDQGLERNRTYYYKIRSFNQRGQQSEFSAVIPAETALTPNPPVILSAEG
                     HVRSIQMIWSPSPMTSDDPLKMKGYKLYRAKVEQGPYREVANILGKDLGIGVDTTTAL
                     DKLFKVPYLDKGLADGEEYFYRVTAYNEKNLESEYSRSVKGTTIPAVSGLIAQGDLIR
                     EIRLSWNPVPSPFIKGYFIYRSLREEADFLKIRKLDAAGARADGRVAFTDTEGLADQV
                     RYYYRVTAVEDTEIETSPSVTVSAVTKGKPPKPEGLQARSGLAKRVDLAWTSSRQPDV
                     VGYNVYWSKEKIGKYLLIKRIEGQANSAFTDTGSAASEKLADEVTYYYALTTFNKVDV
                     ESDLTDPVSATTKARPAKPLGLKAADLQVKQAPLSWDPNPEEDVEAYAVYRLEGTGGG
                     EFSRVATVGKTNAYIDQNLKDGTVYQYRIQAEDRDGLLSDLSDIVRIQTKPRPKVPSV
                     AKGRISGGQAELTWQPNEESDIVHYVVYEKRWYGTEKITTVKETRFSEAGPAKGKKKT
                     YVVTAVDKDGLESDPCPELLIVGR"
     gene            11098..11424
                     /locus_tag="PLW16_08485"
     CDS             11098..11424
                     /locus_tag="PLW16_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70983.1"
                     /translation="MKPIFHRVGRILVFLALVGLIGVCGAGAADTIGKYQVVPGNEDQ
                     AFLVDTTTGAVWILTHRTMATGREPVAIPYKFIRISPQHRGEFLVELAPGGAPAKGGD
                     AKEQTP"
BASE COUNT         2494 a         3370 c         3682 g         2177 t
ORIGIN      
        1 cttcaatctg gtaaccgtcg acaagctcct tattgcctaa gacatcgagg aataacttgc
       61 tcatcatgcg tcaccgatcc tttgctggtt cagtccgatt gatggttcct tcctctcatc
      121 cacaaaaaaa ccgatgcgcc attataaaaa aaaattctca ttcattcaag gattagttgc
      181 atccggcatc gcttgaccat tccgcttcga aatccagtgc ccgaggattg aaaaccatgc
      241 ctgccggatg gaagggcaca acggggaagt cccgccagtt cttgctttcc acatccgaag
      301 gggaaggccc gcaccctcca cggtttgtca atcgcgcgct gatcggaaga aaaatcaaga
      361 tcgccggttt gtaaattttc caacatgaag ctgaacggaa cggcttcatc gtctgctctg
      421 aaaccgtcgg aaagtcaagc ggagatttca cggatgatgc atcggggcac atccctccag
      481 gcgtgccagg gagcgttttc cggccgcctt atcccccgcc cctcgaacga agcgaggggc
      541 gggggaggga aggttcggcg cgcggaggga cggcgatttc aaagggtcga cacctacaag
      601 gggatgttgt tgtgcttctt gtcaggcctg gattcctgct tgttctcgag agtctcaaac
      661 agcgccacga cccgcttgcg cgtatcccgc ggggcgatga tctcctcgat gatgcccagg
      721 ctcgcggcga agtacgggtt gttgaactgc gtctcgtagg cggcgacgag ctcggcccgc
      781 cgggcggccg gatcctccgc ggcgccgatt tcccggcggt agacgatgtt gcaggccccc
      841 tccgcgccca tgacggcgat ctccgccgag ggccaggcca tgacgtagtc ggccccgagc
      901 tgcttgctgc acatgccgat gtaggccccg ccgtaatcct tgcgggtgac gaccgtgatc
      961 ttcggcacgg tggcctcgga gtaggcgtgg agcagtttgg cgccgtggct gatgatgccc
     1021 gaccactcct gctgggtgcc gggcatgtag ccgggtacgt ccgcgaaggt cagaagcggg
     1081 atattgaaag cgtcgcagaa ccggatgaag cgagaggcct tgtccgaagc gtgcgtgtcg
     1141 agcaccccgg cgttgaagcg cgagttgttg gcgatgacgc cgacgggccg gcccatgatc
     1201 cggatgaagg cgacggtgat gttcctcgcc cacagctcgt gcgtttcgaa gacctcgccg
     1261 ttgtcggcga cggccgtgat gacctttttc atgtcgtagc cccggctcga tttgtccggg
     1321 atgatcgtgt ccagttcggg gcattcccga tcggggacgt cggtgcaggg tctgacgggc
     1381 agcgggctgt gacagctgtc cgggaggtag gacagcaggg tcttgacctt tgcgagacag
     1441 tcctcgtcgc tctccgtggc gaagtggcag acaccgctct tcgtggcgtg ggcgacggcg
     1501 ccgcccaggt cgttgtgcgt cacctcctcc ccgatgaccg ccttgatgac gtcgggaccc
     1561 gtaatgtaca tgtagctccg atccttcacc atgaagaccc agtccgtgag cgcgggggag
     1621 tagacggccc cgccggcggt ggggcccatg atcgcggtga tctgggggat gtatccggag
     1681 gccagggtgt ttcggtagaa gatgccgccg tagccttcga gggaggggac gccctcctgg
     1741 atacgggccc cgccggaatc gttcagggag acgaagggtt tgcgggcggt cagggccatg
     1801 tccatgacct tccagatttt ctttgcgtgc atttcgccga ggctcccgcc ggcgctggtg
     1861 aaatcctgtg ccgcggcgaa aaccgtccgt ccgttgacat gaccgaaacc cgtcacgacg
     1921 ccgtcggcgg gaatctcctt gtcgcccaag ccgaagaggg aggagcgatg tttaacgaag
     1981 agctgcacct cctgaaaggt tccgggatcg aagagcatcg ccaggcgttc ccgggcggtc
     2041 agttttccgg cctcgtgctg cttggcgatc ggtcccgcgc cgcccatctc cttcagggcg
     2101 tcccgcttcc gctcgaaggc ctcgatcttt tcccgtgtcg cgcttttctt gagttccttt
     2161 tccatgccgt ctgtcatcct ttttcctttt tttgtcgcgc cttccacaag gcggcgcaat
     2221 tcatcttgac ctgaatgccg ccgtgttata tagcagcaca cctaacgatc cgcagtaaaa
     2281 acctcacaaa aacgccaagg aaatcttggc agtcgcggat tcgggctgct tcgacaggca
     2341 cggcccttat aacccttttc gagacgctgg gcaaggagga tccatgaaat acatcgacga
     2401 gatcgacatc aaggggaaaa gggttttgtt ccgcttcgat ttcaacgttc cgctggacag
     2461 ttcacagaac attacggacg atacgcgcat ccgcgccgtc ctgccgacga tcaactacgc
     2521 gctcgacgaa ggcgcccgaa tcatcatgat ggcccatctg ggtcggccca agggaaagtt
     2581 gatgcccgaa atgagcctgg cccccgtggc caggcgcctc tcgcggttgc tggggaaaca
     2641 gatccctctc gcgggcgatt gcctggggcc ggaggtggac cggatggtgg gcgagctgca
     2701 gcccggctgc ctgatcctcc tggagaacct tcgctttcat cccgaagaga cggccaacga
     2761 cgacgccttc gcgaagcggc tcgccgccta tgccgatgtg tacatcgacg acgccttcgg
     2821 caacgcccat cgcagtcacg cctccaacgt cggcatcacc cggcacgtcc cggtctgcgg
     2881 cgcgggcttc ctgatcaaga aggagctgaa ctatttccgg cgggccctgg agaagccggc
     2941 gcgtccgttt gtcgccatca tcggcggatc gaaggtctcg gggaagctcg aggccatggt
     3001 gaacctgatc aagaaggtcg acaagatgat catcggtggc gggatggcct tcacattcct
     3061 caaggcccag ggctacgaag tcggcaagtc catcgtggag gatgatctca tcccgacggc
     3121 ccaggatgtt ctgcaaacgg cccgggagca cggcgtcaaa ttctacctac ccatcgattg
     3181 tgttatcgcc gaggcgatga cggcggaggc ggagacgaag attgtccccg tgcaggagat
     3241 gaacgcgcac tggatgggac tggacatcgg cccggcgacg atcaccctgt ttacggaggc
     3301 cctggggaat gccaagaccg tgatttggaa cggccccatg ggggttttcg agatcgatgc
     3361 cttcagccgg gggacctatg ccttggcgca cagcgtcggc aactcgtacg ccctgacaat
     3421 cgtcggtggc ggcgatacgg acgtggccat ccaccgggcg ggggaaagcg acaagatcgg
     3481 gtacatctcc acgggcggcg gggcctccct ggagcttctg gaaggaaagg ttctgccggg
     3541 gatcgaggcc ctcgaacggt gcggatcctg aaaggagcgt ttgcatttcg gtcgtttgaa
     3601 cggggagggg aagcgaaact tcccctcttt attttaaggg aatgccttct cgaatcgcgc
     3661 gcccgcggat tcacccccgg cgtatctccg cgcttccagg gcattgcgaa gcggtcgtga
     3721 cccagggtgg aattgaatga aaagcacgga ggtcgggccc cggagggagg gatggatggt
     3781 atgcgaaccc tgaagatcgt gattgcgtac gacggcgccc gctatcacgg ctggcagcgc
     3841 cagctcaacg gctcgtccat ccagcaggtc ctggaagagg ccgtcggccg gatcacgggc
     3901 cgtccggcga cggtggtcgg ctccggccgg acggatgcgg gcgtgcatgc cctggcgcag
     3961 gtcgcccact tccggaccga gtccggcctg gctgaggcga atcttttaaa aggcatcaac
     4021 agcgttctgc ccaaggacat cgcaattacg gatctccggg aggtgccgga aggcttccat
     4081 gcgcgcttcg atgcggcgag caaggtctat ttttaccgga tcgtcaaccg gtcggtgcgg
     4141 gacgttttcg agcacgggcg ggcgtggttc attcatcgtc ccctggccgt cgagcaaatg
     4201 cggcaggccc tgcctcattt tctgggcacc cacgacttta cctccttctg caccgtccac
     4261 tgtgaaagcc gcgaccggat ccggaccgtt ctcgacgccg cgctgaccgg cgaggccggg
     4321 gacatcatcg agacaagcat cgaggccgat ggattcctgc ggtacatggt ccgcaccatc
     4381 atgggcgttc tggtcgatgt gggactgggc aagcatcccc ccgaggccgt gggggagatc
     4441 ctgcaggcca gggatcgttg ccgtgccggt atgacggcgc ccgcccgcgg tctttttctg
     4501 aaggaagtca ggtacggaca cgtatgaaga tcctgatcat cggtcacaaa ggcatgctgg
     4561 ggagcgatct ggtactccgt ttggccgcgt ccggccatgt cgtgtcgggc aaggacgtcg
     4621 gtgacgtgga catcacgtcc ccggcatcct gccgggcggc gattgcggaa tgcgatccgg
     4681 aaatcgtgat caacgcggcg gcctacacgg atgtggacgc ctgtgaggcg aatcccgatc
     4741 tgtgttttgc cgtcaacgcg gagggcgtca ggcatgtcgc catggcgtgc cgcgagtatc
     4801 gggtgaagct cgtccatttc agtacggatt atattttcga cgggaccaag gggcagccct
     4861 atgtcgagga ggatcccggc cgtcccctga atgtctacgg cgcctccaag ctggccgggg
     4921 agcgttacct gatcgacctg gccccggact tcctgctgat ccgaaccgcc tggctgtacg
     4981 gccgccacgg taaaaatttc gtccggacca ttctggcggc ggcacggcgg cggccgacac
     5041 tggacgtcgt ggacgatcaa gtcggctctc ccacctatac ggtcgacctg gcggcggccg
     5101 tcgaacggct gatcttcgag gaccggcacg gggtcttcca cgtcacgaat cggggccagg
     5161 caagctggtt cgactttgcg aaaaccattc tcgcccatgc cggattgacc gacgtcgcgg
     5221 tgcgtcccct tgcgtcaacc cggttgggac gcccggccag acgtccgtct tacagcgtca
     5281 tgagcaaccg gaagttcctc cagacgacgg gccggacgat gcgattctgg cagattgcgc
     5341 ttcaggacta cctgtcctcc gtgtcaattc tggagcagtg gagcccgtcc gccgaaaagc
     5401 cttgacacct tagagagatt tcagtatatg cggtgcaggt tcgttaacct gccgatttat
     5461 cgagcggaac gaccgttcaa ggccgccggg gaccggcggc gggaaaggac gcgaactggc
     5521 atgatgagaa gatgcatact tttcgtgacg ctcgtgggac tgatgttcgg gggggtaagt
     5581 ctcctttatg cccagaaccg ggtcgtggcc gatgggatgg ccgtcgtcaa caacaaccgc
     5641 gtggacatcg cccgcgacag ggcggtcgac aacgcccagc gcaatgccgt ggagcgggtg
     5701 gtcggggtca tgatttccag cagcacggag gtggagaatt ttcaggtcaa gatggaccgg
     5761 atcctttccg agtcgcaggg attcatcaac tcgcgcaaaa tcgtttccga acggcgtgac
     5821 ggggacatgt acgaagtcac catcgaagcc gatgtgggga tgggaaaact gaaggaccgt
     5881 ctcgccgccc tgaacctcat catggcccgc aaggaaaaac cccgcctgat gcttcttttc
     5941 agcggacggg cacagaagga cgccgttgcc gagtccgcga tgtcccgcta tttcctgtcc
     6001 caggggttca agctggtcga cgccgatacc tacaaaaagg gcacggaacg cgagaatctt
     6061 caggggctgg cgctcaatcg gcgggagacg gccaatttcg gccatcgtta cggtgcggaa
     6121 gtcctgatcg tcggcgatgt cgaagcgtcg tccagctcgt cgagattcgg ggagatcgaa
     6181 atgacgacga acaaggtgat cgtctcgacc aagatcatca acgtcgatac gggcgaggtt
     6241 ctggcgacgg acagcgaaac aagatcgtgt ccggggatgc gcgacgacat caaacagatc
     6301 acggtggatg ccgcggacag cctggcgaag aagatgacgg acgaaatcat ggagcgctgg
     6361 tcgtcagagt tgaccaatgc ggttaccgtg aaactgatcg tgaccgggtt taacgcctat
     6421 caggatctat tgcagttcaa ggaacagatc gcgagcgacg tgagaggatt ccgggagatc
     6481 tatcagcgcg cctatgccca tggtcaggcc gaactggatc tcgagatcaa gggcaatacc
     6541 caaggggtgg ccgacgacct ggccgccatg accttcaacc agcgtaaatt gagaatcacg
     6601 gaaatcaccc agaaccggat cgtggcgaag ctggtcccct gaaagggacg ttttctgccg
     6661 gccccacggg gattcgtgga acgcaaacga cggtcaagat gcaccacagg gaggtaatca
     6721 tgaaacgggc gatgagaaca cggatgcttt cgggattgtt tgtcctgctt ttgcttgccg
     6781 gatgcgccgt cagcgagagt ttcaaggtcg gccaggatct gagcggcagc cagcgctggg
     6841 acgaggcgat catctatttc cagaaggcgg tcagtgaagc gcccgacaat caagagtaca
     6901 aagacgccct tgaaaatgca aaaagagagg cggccaaggt tcacctggcg cgggcccggc
     6961 agcttctcgc cgaggcgtcc ccggaagtga cggtcccggc tttggagcgg gttctcaagg
     7021 aagcggatac cgccgttgcc ttggaccccc agaacaagac cggtgttgcg ttcaaacagg
     7081 aagtgggcgg ggaaatggag gcgcttcagg gcaaactgaa gaccctctac ggacaggccg
     7141 agctggacat gcaaaaagag gactggccgg cggcgttgac caagctcaag caggtcaacc
     7201 aactcttccc gaactatgag gatacgggta accggctcgc ccgctccgag caggaagccg
     7261 tcaagagcct ttaccaacag ggaattgccc tgggcaagca ggaggaatgg aagctggcgg
     7321 cccaggtctt caaggcggcc gtcgagatca atcccaacta ttacgacctg tccgcactgg
     7381 ccgcgcaggc gcagtcgcgc gacaatgtcc aatactacct gacggaggcc gacaaagcag
     7441 ccggcgtcca gaactgggac cgggcgatca cgctctacga aaaggcgctg gaatatcagc
     7501 ccgaaaacca ggaactggtt gggaagatgg acggcatgaa ggccaaggtg gggcagctct
     7561 acttcgacga agccgtcaaa ttcatgaatc agggcaagta ttacccggcc atgaaacggg
     7621 tggaacggat ccggacctac gccccggagc tgcaggacga tccggttttc aaggaatttc
     7681 tctggaattt ctgcaagcag ctcatggatc gggccgacaa atacgcgcaa aaggaactct
     7741 gggggaacgc gctggtctgg cttgagaaag tgcaggccat caatccctcc tatccggaac
     7801 tgttccagaa ggtcctggat accaaggatc ggatcaacaa acggatcaag aaatccatcg
     7861 ccgtctttga cttcagcagc ccgagcaacg acaaggacgc gggcaagatt gccgccaaca
     7921 agctgatcac gttcctgcac aagaacggga gcggggatct caggatcatc gagcgggaaa
     7981 atcttcagtc catcctgcgg gagatgcaac tggggcagac ggggctggtc gatgtcaagt
     8041 cggtccagag cgtggggaag atgaggggga tcgatacctt catcatgggc gacgtgctgc
     8101 acttctccgc gcgtacgacg gatatgccga gcctgggtca ggtcaaggtt ctggtcaatg
     8161 aagaggatgt ccgcaatccg gagttctccg actggctgat gatgaacccc aagcccaacg
     8221 cggacgatct caagcacgcc cccccacgga cggtgaaaaa gagagattac cagttcatct
     8281 cctacaaaca gggatcgagc aaggtcaccg ccattatcga gatttcatac aaacttgtgg
     8341 ataccctgac cggggagaac atcttcacca acaccatttc cggaagactg atcaaggagg
     8401 acaagtacca ggacggcgtt gcgattgcca atatccccca cgatccgctc gagattccga
     8461 cggaggtgga ggtcctggac gagttgacga acgagaagat ctcggagatg ggccagagcg
     8521 tgctcaaaca ttaccagagc ctggaggtcg aatacttcaa ccatgggcag cagcagcaaa
     8581 aaaggcgcaa catcgatcag gccgtcgaac ggtacaccga tgcgatctac gacgagaagc
     8641 tgaagggcat ctcgaccccg atttcccaga aatccatgga gttcatcgtc aagctggtgc
     8701 aggataaata gccaaggcga agcgcggggg gcccggacgt cgagccgggc ttttctgcct
     8761 ggagggaaac catgaagaac agaaggttcg tatccttctt cccactgctg tggacgatcg
     8821 ttttttccct ccccgtgctg gcagcggcgc agccccccac ggcgacccgg gtttccgttt
     8881 tcaatttcgg cacggtcaat atcgaagcct ccggctacgg gaccaccgtg accaacatgc
     8941 tgaccacctc cctgcagtcc gaagcctccc tgaatctgat ggaccggaag gagttggagg
     9001 cttttctcag tctcaacgac ctgcagcaga acgacctctt cgaaaacgtc atccacatcg
     9061 gaacgcgcct ggggcttaac gtcatcgtcg tgggcaacgt gcagaagcgg ggaacgatta
     9121 tcgccatcca ctgtaaggtg gtgcacatcg agcagaaacg ggtgatcttc cagacccatg
     9181 tccgggccct gggcgacgcc ggtctcctgg gcgaagtccg gaaactcgcg tccatgatcg
     9241 tggcggccat cgcggaaaac gccagccgtt cgctggagga caagccctcg atggggggac
     9301 ccgtcaacgt acagaagcgg tcggggaaca tgcgtgtttc cctgaactgg gaggatcccc
     9361 ccgacgcgcc ggccgccggt tacgaactct ttcggagctc cacggagacc ggtcccttcg
     9421 ccaagatcgg ccagacaacg cgccccgaat atctcgacca gggcctggag cggaacagga
     9481 cctactatta caagattcgt tccttcaatc agcggggtca gcagagcgaa ttttcagccg
     9541 tgatcccggc cgaaacggcg ctgactccca acccccccgt gattctgagc gccgaaggtc
     9601 acgtgcggag catccagatg atctggtcgc ccagtcccat gaccagtgac gacccgctga
     9661 agatgaaggg gtacaaactc taccgcgcga aggtggaaca ggggccctac agggaggtgg
     9721 ccaatatcct gggcaaggac ctggggatcg gggtcgacac cacgaccgct ctcgacaagt
     9781 tgttcaaggt tccttacctc gacaagggac tggccgacgg ggaggagtat ttttaccggg
     9841 tgaccgccta caatgaaaag aaccttgaaa gcgagtattc ccgaagcgtg aaagggacga
     9901 ccattcccgc cgtctccggt ctgatcgccc agggcgatct catccgggag atccggctga
     9961 gctggaatcc cgttccgtca cccttcatca aggggtattt catctatcgc agtctccgtg
    10021 aggaggccga ttttttgaag atccggaaac tggatgccgc cggcgcccgg gcggacggga
    10081 gagtcgcgtt tacggatacg gagggactcg ccgatcaggt ccgctattac tatcgcgtca
    10141 cggccgttga agacaccgaa atcgagacgt ctccttccgt gaccgtttcc gccgtcacga
    10201 aggggaaacc cccgaaaccg gaagggcttc aggccaggag cgggctggcg aagcgggtgg
    10261 atctcgcctg gacgtccagt cggcagccgg acgttgtcgg ctacaacgtc tactggtcga
    10321 aggaaaagat cggcaagtat ctgctgatca aacgcatcga aggacaagcg aacagcgcct
    10381 ttacggatac cggttccgcc gcttcggaaa agctggcgga cgaggtcacg tattattacg
    10441 ccctgacgac gttcaacaag gtggatgtgg aaagcgatct gacggatccg gtttccgcga
    10501 ccaccaaggc ccgtcccgca aagcccctgg ggctcaaggc tgccgatctt caggtgaaac
    10561 aggcgccgct gtcctgggat cccaatccgg aggaggatgt cgaggcgtac gccgtctacc
    10621 ggctcgaagg aaccggaggc ggggagttct cccgtgtggc gacggtcggg aaaacgaatg
    10681 cctacatcga ccagaacttg aaggacggta cggtttacca gtaccgcatc caggcggaag
    10741 accgggacgg gctgctgagt gatctgtccg atatcgtccg catccagacg aagccgcggc
    10801 cgaaagtgcc gtcggtcgcg aaggggcgta tcagcggcgg ccaggcggaa ctcacctggc
    10861 agcccaatga agagagcgat atcgtccatt acgtcgtcta tgaaaaacgt tggtacggaa
    10921 ccgagaagat caccaccgtc aaggaaacgc gcttcagcga agcgggaccg gccaagggga
    10981 aaaagaaaac ctatgtcgtg acggcggtcg acaaagacgg gctggaaagc gatccgtgcc
    11041 cggaactttt gattgtagga cgttaatcaa agcggggtga ggcgggggat gaagacgatg
    11101 aaaccgatat tccatcgagt gggcaggatc cttgtgttct tggcgttggt ggggctcatc
    11161 ggcgtctgtg gagccggagc ggccgatacg atcgggaaat accaggtggt gccgggaaac
    11221 gaagaccagg cctttctggt cgacaccacg acgggcgccg tctggatcct gacccaccgc
    11281 accatggcca cggggcggga gccggtcgcg attccctata aattcatccg gatctctccg
    11341 cagcaccggg gcgagtttct ggtggaactg gcgccgggcg gtgcgccggc caagggcggc
    11401 gacgcgaaag agcagacccc ctgatccggg ggatgccggc cggcgttgaa cgcggaaaag
    11461 acggaccgtc atggaggttc atccgattcc atgcagcggc tgctcgtgat tgaaggcggg
    11521 gcaaacgccc tgctcaccgt ggcgcaaact tgaggttttg ggaggccagc catgaacgcg
    11581 ttcaagcggt tatccctttg tctgacagtg gtgctcgcga ccttggccgt gcaaagctgc
    11641 gtcgtggcca ctaagacgac gatcatcact ccggagatca tcgccctgta caagggaaag
    11701 ttcaagatcg acccctacat gca
//
LOCUS       DAOOLJ010000060        11504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_6291_length_11504_cov_16.046467, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000060 DAOOLJ010000000
VERSION     DAOOLJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6291_length_11504_cov_16.046467"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /locus_tag="PLW16_08490"
     CDS             <1..2174
                     /locus_tag="PLW16_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HPB70984.1"
                     /translation="TVDHPEYGGDDTITITGDGNNHILGGMGSDTISTAATTAGTTDV
                     ILGDNGIVTYAGATGSLELKKIVSTVPSQGGADTILTGDGDKHIIAGYGSDAVTAGTG
                     DHVVFGDAGQADYDTDGAVLNLQTTDTVDHPEYGGDDTITITGDGNNHILGGMGSDTI
                     STAATTAGTTDVILGDNGIVTYAGATGSLELSQIVSTVPSQGSESGLVSGDDVIVTGD
                     GDKHIIAGYGSDTVTAGTGDHVVFGDAGQADYDTDGAVLNLQTTDTVDHPEYGGDDRI
                     TSGDGNNLILAGMGADTVTEGNGTGVILGDNGTILYEGPTGNLQISQVVSTLPSSGGG
                     DTLTSGSGDKYVIAGVGADTVATGSGNDIILGDNGIILSGPDGIVDLVQSTSPTIGAA
                     DVVTAGEGNNILIGGTGADRMSSGSGKDILLGDGGQVTFSAGMLLYIETIDNFIGGND
                     FLDGGPNEDILFGGAGNNTFVGTLADDVMVGSYGRVTFEGGAVDMVIRLDSTDIIANT
                     MTDLQGGKSAVRDMVPEAVYGEDDEEWGTGLVGEDAQYAAFYPENRGYESVGTGGVAG
                     SGMMERGTPGVLSASNNHHGDDLSEERGEAGPSVMEPEEQPAAGETEARAPREEMIEG
                     QPSGSSGERPAASSQAEDQLVEEPSGLDEGGTKSDPNAGNTGGIDSAAGKAIAGLMGW
                     KIISGGDLDKSSIVNTKSFERLAKKQEDKRYKRWKNAGSWQ"
     gene            2266..3375
                     /locus_tag="PLW16_08495"
     CDS             2266..3375
                     /locus_tag="PLW16_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70985.1"
                     /translation="MAGNLRAEIIQVHTEPGEFHFLVRGRPVEGNGSPLTVGPVDRRI
                     ILSPSTAKGLVRLLEAALRDYETAHRPESAPDQSTVEGGREPGQTGRRSILESVRGEE
                     ARRLIQSLDGLGVHYGFEQSFKVLPGLILGNRFLAGFNRDSVRQGLDEKMEVICRKLG
                     MPESQMGEFIRGFPEANILLFGYEQGEKESVYKAYLEFGREFERISRENPSNRSPFLI
                     HRGFKWDIADKARHTTANYMCYPHRSVEELLGRLSDVFYSGTPGMPFDIVRGLVELAS
                     ARMRPDEFLYLEVAEEGNARLSFDVNMYRANLRVSELYPLLMAMCRYFSVPLADFHRI
                     YESVKDLIFGHISGGVDRHGRDFLTVYYGVKGSTL"
     gene            3451..4434
                     /locus_tag="PLW16_08500"
     CDS             3451..4434
                     /locus_tag="PLW16_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB70986.1"
                     /translation="MERMCGQVVERDVKTGRVDLSKYLVISVWFGCNNDCTICMLSDL
                     KRSLPPIGFEKFSQVVSDVVEHRRFDNLILSGAEVTTFDDLERYVRFAASFGWFQRIQ
                     IQTNGRRLSDRRYLDRLVACGVNEFFVSLHGFDEVHDALTRRPGSFRETLAGLRNLEG
                     LTDVNVISNTVLNRTNVHQIAELLHFLSGLRIGELHLWNLFPMEETDRRDLVVSIREF
                     ARLLPDLLARLKPAGKPIVLKSFPECLAAGSPAVFDDWFPVTVLPNRFWEEFQRSGFG
                     TCIYRDRCAAAETCWGLSRAYLRKYGDERDILAPVGRKERPADGRPREAIP"
     gene            4431..5291
                     /locus_tag="PLW16_08505"
     CDS             4431..5291
                     /locus_tag="PLW16_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70987.1"
                     /translation="MTRRERMTDRDLFFNYCLWAYEPVAPWQDKWRSANLLFHSFERA
                     GADDRMFDLVERLRGTVGPSMTVWGVKWAGGKIGWEYYFYDYRRRERERSAAKVLQGM
                     APLVRSSVRLNENHHYFMFSLDIDDALVTGARSVDEIHMYIGNPGSDVSSGICYAVTP
                     AGSRLENFYFFFDARRHREDILNKIACSAQIDQGAVELGEIYRPELRDCRTICLANKQ
                     RTDCIYFSGITVDQLIFFLNWLDYPRSLCSLVERNRSRLDHLLYDVGFDYRMESDRLR
                     IEKSGYYGIF"
     gene            5373..6428
                     /locus_tag="PLW16_08510"
     CDS             5373..6428
                     /locus_tag="PLW16_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB70988.1"
                     /translation="MNGHETGPDAPREREFDFLRVDEFLRSLLPSCALGVALQSGLID
                     FLVQNGPAAAETLADRFAADAKGMRLLLDQLLANRVLTDDHGRFALSEDFLKALSYRD
                     LMELKLEMSNLGVFDLLRFFPDLVFRPGEFARKSDFCGLFAYDRCFEKSEESVRLTRR
                     WMRITTVLTKYEAQACLKYHDFGGYRRILDVGGNSGEFALRICRSHPRAEVTVFDLPL
                     VCEIGAAHVQREPEAKRISFVKGNALGDPWPSDFDLVSFKSTLHDWPEEAAGRLMTSA
                     AEALRPGGTLLVFERGPLETSGTPVAYAHIPFFLFQHSFRSPRFYTQRMQALGFRNIG
                     VRILDLGTPFLLVTGVR"
     gene            6547..6891
                     /locus_tag="PLW16_08515"
     CDS             6547..6891
                     /locus_tag="PLW16_08515"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPB70989.1"
                     /translation="MAQKSDKTVQAVIPETDAGNVKAKIRWDVGKMQTTYANVCNVSS
                     TREEFTFLFGINKAWNPEQRELTVDISDRVILNPYAAKRLSLLLRNIVRQHELRFGEI
                     QLKPVAKPEENA"
     gene            complement(6955..7593)
                     /locus_tag="PLW16_08520"
     CDS             complement(6955..7593)
                     /locus_tag="PLW16_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70990.1"
                     /translation="MVSTERHVIGIAGPAGAGKTSLVRALAERLDDAVVIHYDHYERA
                     TQEPMDRLAQWIRAGADVNDIRLPELARDLGALKRGEPVINPVTRMELTPRRHIIFET
                     PLGREHRETGRHIDLLLWVDVPLDVALARKIREFASVVLARGDPRNIQDFLAWLHTYL
                     ENYLGGIHDLMDLQRERIRANADRIIDGNRMFETVLQEILKTVRSDPSSGRF"
     gene            7866..8210
                     /locus_tag="PLW16_08525"
     CDS             7866..8210
                     /locus_tag="PLW16_08525"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPB70991.1"
                     /translation="MVQRKDADIEKKALGEGAEDDQDNAKATIQWDVSKMESSYANVC
                     NVSCTREEFTLLFGMNKTWDAGQRKLTVDMTDRVIVNPYAAKRLYLLLGNVIRQYEDR
                     FGEIHLDVAEEK"
     gene            8671..10497
                     /locus_tag="PLW16_08530"
     CDS             8671..10497
                     /locus_tag="PLW16_08530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB70992.1"
                     /translation="MDIAKAWEGLQSVQDRDEFVAAWLTVQCSMIRGCIQGVLVLMDS
                     AAGTYMPVASWPEKGSFERLSDILNRTLSEKEGMLVELNPSDAIPDEDDRRYALAYPI
                     VVDELLIGSVAVEVAASSDASLRRAMEELQWGAVWLENLNRREQGIEDRDTLKRLKAA
                     VDILAVVLAEGSFQEAATAFVTEVATRLSCDRVSFGLVKGSSVRVKAISHTAIVGKKM
                     NLVRSIGAVMEEAVMQRTEIVYPPPPDAEPAVVRDHERMIQVHGDRSMLTLPLYAHDR
                     YYAALTLERQDDRPFTKDEQSYIKSVAALTGPALENKFQNDRAIVFVILASLKRQAEK
                     IFGAGYVGRKVAILAAVLLIVFFSLARQDYRLTADAVLEGSVTRSIVVPMDGYIQESF
                     VKAGHTIPKDAVMCRMDGRDLELERQNWLGRKRQYERQYQEAVAKHNRAEASIISAQL
                     DQAEAQIALVDKKRSQIVIRAPFTGIVIRGDLSQRLGGTVAKGEEIFQMAPLDAYRLI
                     LKVDEQRITDVSEGKKGVLVLSSMAGHPFGFTVRKITPITTAAEGRNYFRVEADLEQA
                     SPRLRPGMEGVGKIDVDRRRIISIWTRDLIDWFRLELWSWLP"
     gene            10507..>11504
                     /locus_tag="PLW16_08535"
     CDS             10507..>11504
                     /locus_tag="PLW16_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB70993.1"
                     /translation="MSEKTLYSNSWYRVADLKPFVRNHAEIHRQDYRGQAWYVLEDHA
                     SGRFHRFTEETYFIIGLMNGERTLQEIWEAACNKLGEDMPTQDEMILLVSQLHQADVL
                     QADIAPDIENLHRRQQKDRKTRFWNAVRSPLALRLPILDPDRFLEKTVGLVRPFYSLP
                     AAFVWLVIVTSAVVLAVTHWQELTSNLADRVLLLENILVFWMIYPIIKVFHEFGHAYA
                     VKRWGGEVHEMGVMFIVFMPIPYVDASSSTAFRDRRKRIVVGASGIAVELLIASLAMI
                     LWLNLEAGAARAIAYNTMIITGVSTLFFNGNPLLKFDAYYVLCDLIEIPNLGTRANR"
BASE COUNT         2297 a         3302 c         3703 g         2202 t
ORIGIN      
        1 acacggtgga tcatccggag tacggcgggg acgacacgat caccatcacc ggagatggga
       61 acaaccacat cctgggcggg atggggtctg acacgatcag cacggcggcg acgacggccg
      121 ggacgacgga cgtgatcttg ggcgacaatg ggattgtgac gtatgcgggt gcgaccggat
      181 cgctggagct gaagaagatt gtttccacgg ttccgtcaca gggtggggct gatacgatcc
      241 tgaccggcga cggcgacaag cacatcattg cgggttacgg gtccgacgcg gtgacggcgg
      301 ggaccggtga ccacgtggtg tttggggatg ccggtcaggc ggattacgac acggacggcg
      361 cggttctgaa cctccagacc acggacacgg tggatcatcc ggagtacggc ggggacgaca
      421 cgatcaccat caccggagat gggaacaacc acatcctggg cgggatgggg tctgacacga
      481 tcagcacggc ggcgacgacg gccgggacga cggacgtgat cttgggcgac aatgggattg
      541 tgacgtatgc gggtgcgacc ggatcgctgg agctgagcca gattgtttcg acggtaccct
      601 cacagggtag cgagtccggg ttggtgagcg gcgacgacgt gatcgtgacc ggcgacggcg
      661 acaagcacat cattgcgggt tacgggtccg acacggtgac ggcggggacc ggggaccacg
      721 tggtgtttgg ggatgccggt caggcggatt acgacacgga cggcgcggtt ctgaacctcc
      781 agaccacgga cacggtggat catccggagt acggcgggga tgaccgcatt acctcgggcg
      841 acggaaacaa cctgatcctg gcggggatgg gggccgacac agtcacggaa ggaaacggga
      901 cgggcgtcat cctcggcgac aacggcacga ttctttacga aggtccgacc ggcaacctcc
      961 agatcagcca ggtcgtctcc acgctgccgt ccagcggcgg cggggatacg ttgacgtccg
     1021 gaagcggcga taaatacgtc atcgccgggg tcggcgcaga caccgtcgcg acggggagcg
     1081 ggaacgacat catcctgggt gacaacggga tcatcctctc cggcccggac gggattgtgg
     1141 acctcgtcca aagcacctcc cccacgatcg gggcggcgga tgtggttacg gccggtgaag
     1201 gaaacaatat cctgatcgga gggacgggcg ccgaccggat gagcagcggc tcggggaagg
     1261 atatccttct cggcgacgga gggcaggtca ccttctcggc cggaatgttg ctatacatcg
     1321 aaacgatcga caatttcatt gggggaaacg acttcctgga cggcgggcca aacgaggaca
     1381 tattgttcgg cggtgccggc aacaatacct ttgtcggcac gctggccgac gacgtcatgg
     1441 tggggtccta cgggcgggtc accttcgagg gaggcgccgt cgacatggtg atccgactcg
     1501 attcgaccga catcatcgca aacacgatga cggacctcca gggcggaaag agcgccgtgc
     1561 gcgatatggt accggaagcg gtgtacgggg aggacgatga ggagtggggg acaggccttg
     1621 tcggcgagga tgcgcagtac gcggccttct acccggaaaa ccgcggatac gaaagcgtgg
     1681 gcacgggggg tgtcgccggt tccgggatga tggagcgcgg gacgcccggc gtgctttcgg
     1741 catcgaacaa tcatcacggc gatgacctgt ccgaagagag gggagaagcc gggccatcgg
     1801 tgatggaacc ggaggaacag ccggccgccg gagaaacgga ggcgcgggcg ccgcgggagg
     1861 aaatgatcga agggcagcct tccggctcct ccggagaacg gccggcggca tcttcccagg
     1921 cggaagatca actggttgaa gaaccttccg gcctggacga aggcggcacc aagagcgatc
     1981 ccaacgccgg aaacaccggc ggcatcgaca gcgccgcggg gaaagcgatt gccggcctga
     2041 tgggatggaa gatcatctcc ggaggggact tggacaagag cagcatcgtg aatacaaaat
     2101 cgttcgaacg gcttgcgaag aagcaggaag acaagcggta caagcgttgg aagaacgccg
     2161 ggtcctggca atagggttca acacctgtcg ccgatcgccc cgcccgaaag gcggggcttg
     2221 tttttcgtgg tacgcgagca gcggacggcg tccgagaggg caggattggc tggaaatctg
     2281 agggcagaga tcattcaggt ccacaccgaa cccggggagt tccattttct cgtgagggga
     2341 aggcccgtcg aggggaacgg ctccccccta acggtcggac cggtcgacag aaggatcatt
     2401 ctcagcccgt cgactgcgaa gggtctggtt cgtctcctgg aagcggctct gagagactac
     2461 gagacggcgc accgccctga atcggctccg gatcaatcaa cggttgaggg cggtcgtgag
     2521 ccgggacaga cgggccggcg gtcgattctg gaatccgtgc ggggggagga agcccgtcgt
     2581 ctcatccaat cgctcgacgg tctcggtgtt cactatggat tcgagcagtc cttcaaggtg
     2641 cttcccgggc tgattctggg aaaccggttc ctggccggtt tcaacaggga cagcgtgcgg
     2701 caggggctgg acgaaaagat ggaggtcatt tgccgcaagt tgggcatgcc cgaaagccaa
     2761 atgggggaat tcatccgggg ctttccggag gcgaatattc ttctctttgg ctatgaacag
     2821 ggtgagaagg agtcggtcta caaggcctac cttgaattcg gccgcgagtt cgagcggatc
     2881 tcccgagaga atccctcgaa tcgctccccg tttctcattc accgcggttt caaatgggat
     2941 atcgcggaca aagcccggca cacgaccgcg aattacatgt gttatcccca tcgatcggtc
     3001 gaggagcttc tcgggagact gtcggatgtt ttctacagcg gaacgcccgg gatgccgttc
     3061 gacatcgtcc ggggccttgt ggagctggct tcggcaagga tgcgcccgga tgagttcctc
     3121 tacctggaag tggcggagga gggcaatgcc cggctgtcct tcgatgtcaa catgtatcgg
     3181 gcgaacctgc gtgtcagcga gctctacccg cttctcatgg cgatgtgccg atacttttcg
     3241 gttcccctgg cggatttcca ccggatttat gaatccgtaa aagacctgat cttcggccat
     3301 atctcaggcg gggtcgatcg ccacggacga gacttcctga cggtctatta cggcgtgaag
     3361 gggagtaccc tgtaggctac cgggtcggcg acgcggggag ggcgcagaag ccgatccgtt
     3421 cttggatgat acggtacggc gacctctccg atggaaagga tgtgcgggca ggtggtggag
     3481 cgggatgtga agacagggcg cgtcgatcta tcgaaatacc tcgtgatctc cgtctggttc
     3541 ggctgcaata acgattgcac gatctgtatg ctgtccgacc tcaagcgcag cctgccaccc
     3601 atcggattcg agaagttctc tcaggtcgtc agcgacgtcg tggaacaccg gcgtttcgac
     3661 aacctgatcc tttccggggc ggaggtgacc acctttgacg atctggagcg gtatgtccgt
     3721 ttcgcggcgt ccttcggatg gtttcaacgg atccagatcc agacgaacgg acgccgactc
     3781 tccgatcgcc gctatctcga tcgactggtc gcctgcggcg tgaatgaatt tttcgtcagc
     3841 ctgcacgggt tcgacgaggt ccacgacgca ctcacccggc ggccgggatc gttccgggaa
     3901 acccttgccg ggcttcggaa tctggaagga ctgacggatg tgaacgtgat cagcaacacc
     3961 gtcctcaacc gaaccaacgt tcaccagatc gccgaattgc tgcattttct atccggcctc
     4021 aggatcggcg aactgcatct ctggaatctc ttccccatgg aggagaccga tcggcgcgat
     4081 ctcgtggtca gcatccggga gtttgcgcgt ctcctgccgg acctcctcgc acgcctgaag
     4141 ccggcgggga agccgatcgt cttgaaaagt tttcccgagt gcctcgcggc cggttcgccc
     4201 gccgtcttcg acgactggtt ccccgtgacg gttctcccca accggttctg ggaggaattc
     4261 cagcggagcg gtttcggaac ctgcatctac cgggatcggt gcgccgccgc cgagacctgc
     4321 tgggggcttt cccgcgccta tctccggaag tacggggacg agcgtgacat cctggcgcct
     4381 gtcggacgaa aggaacgccc ggctgacggg cggccccggg aggcgattcc atgacgagac
     4441 gagagcggat gacggaccgc gatcttttct tcaactactg cctgtgggcg tatgagccgg
     4501 tcgccccgtg gcaggacaag tggcgttccg ccaatctgct gttccattcc ttcgagcgtg
     4561 cgggagccga cgatcggatg ttcgacttgg tggagcgcct ccgggggaca gtcggcccgt
     4621 cgatgaccgt ctggggggtg aagtgggcgg gcgggaagat cggatgggag tactacttct
     4681 acgattaccg gcggcgggag cgggaacggt ccgcggcgaa ggttctccag gggatggcgc
     4741 cgctcgtccg atccagcgtg aggctcaacg aaaaccatca ttacttcatg ttttccctcg
     4801 acatcgatga cgccctggtc accggggcgc ggagcgtgga cgagatccac atgtacatcg
     4861 gcaatccggg cagcgacgtt tcctccggca tttgctacgc cgtcaccccg gccggatccc
     4921 ggctggaaaa tttttacttc ttcttcgacg cccgaagaca ccgggaggac atcctgaaca
     4981 agatcgcctg ctccgcccag atcgatcagg gggccgtcga actcggcgag atctaccggc
     5041 cggaactccg ggactgccgg acgatctgcc tggcgaacaa acagcggacc gactgcatct
     5101 acttctccgg catcaccgtg gatcagctta ttttcttttt gaactggctg gactacccgc
     5161 gatcgctatg ttccctcgtg gagcggaacc ggtcccggct ggatcatctg ctgtacgatg
     5221 tcggattcga ctaccgcatg gaatcggacc ggctgcgcat cgagaaaagc ggctactatg
     5281 gcatcttctg acgctcggtt catggtgccg agtagaatcg gatcgaagcg aaggcgcggg
     5341 cgtttctgtg caaatgaagc ggtgcatcgg ccatgaacgg acatgaaacg ggaccggacg
     5401 ctccccggga gcgggaattc gatttcctgc gcgtggatga atttctgcgg agtcttctgc
     5461 cgtcctgtgc cctgggcgtc gccctccagt ccggactgat cgattttctc gtgcaaaacg
     5521 ggcccgccgc cgcggagacg ctggcggacc ggttcgcggc ggatgcgaag ggcatgcggc
     5581 tcctgctcga ccagctcctc gccaatcggg tgttaacgga tgatcacggg cgttttgcgc
     5641 tctcggaaga ctttctgaag gcgctgtcgt accgcgacct gatggaactg aagctggaga
     5701 tgtcgaacct gggggtcttc gacctgctcc gatttttccc ggatctcgtg tttcgtcccg
     5761 gggaatttgc gcgcaaatcc gacttctgcg ggctttttgc ctacgaccgc tgtttcgaga
     5821 agagcgagga gtccgttcgg ctgacccgtc gctggatgcg catcaccacg gtgctgacga
     5881 agtacgaggc ccaggcctgc ctgaaatacc acgatttcgg cggttacagg cgcattctcg
     5941 acgtcggggg aaacagcggc gagttcgccc tccgcatctg ccggagccat ccgcgtgctg
     6001 aggtcacggt gttcgatctg cccctcgtgt gtgagatcgg ggccgcccac gtccagaggg
     6061 agccggaggc gaaacgtatc tccttcgtga aggggaatgc cctgggggac ccctggccgt
     6121 cggatttcga cctcgtgtct ttcaagtcca cgcttcacga ctggccggag gaagcggccg
     6181 gtcgactgat gacgtccgcg gccgaggccc tgcggccggg cggaacgctg ctggtcttcg
     6241 aaagggggcc gctggaaacg tcgggaacgc cggttgcgta cgcccatatc ccgttctttc
     6301 tctttcagca cagtttccgt tcaccccgct tctatacgca gcgcatgcaa gcgctgggct
     6361 tccggaatat cggagtccgg atcctcgacc ttgggacgcc ctttttactg gtgacgggtg
     6421 tgcggtaatc cgacgcggag agccttttct gcggggccgt catgttgaca ggaggcgctc
     6481 aatcggatag aacgtcaagt ggggcgaccc atcccaacct atcaattcat ccaagaggag
     6541 gatagcatgg ctcagaaaag cgataagacc gtccaggccg tcattccgga gaccgacgcc
     6601 ggaaacgtga aggcgaagat ccgttgggat gttggaaaga tgcagacaac ctacgcaaac
     6661 gtgtgcaacg tgtccagcac tcgggaagaa ttcacgttcc tgttcggcat caacaaggcc
     6721 tggaaccccg agcagcgcga actgaccgtg gacatcagcg accgggtcat cctgaatccc
     6781 tatgcggcaa aacgcctctc tctcctgctt cgaaacatcg ttcgacagca cgagctccgc
     6841 ttcggagaga tccaactgaa acctgtcgcg aaaccggagg aaaacgccta ggacccgcgg
     6901 gcgtcgatcc cgactccaca ggcgacccgg ggcgggccgt cgggtaatcc cggatcagaa
     6961 gcgaccgcta gacgggtcgc ttctgacggt cttcaggatc tcctggagca ccgtctcgaa
     7021 catccggttt ccgtcgatga tccggtcggc gtttgcacgg atccgttccc gctggaggtc
     7081 catcaggtcg tgaatgcccc cgaggtaatt ttccagatag gtgtggagcc aggcgaggaa
     7141 atcctggatg tttcgggggt ctcctcttgc gaggaccacc gaggcgaact cccggatctt
     7201 ccgggccagg gcgacatcca gcggcacgtc gacccagagc agcaggtcga tatgccgtcc
     7261 cgtttcgcga tgctccctcc cgaggggcgt ttcgaaaatg atgtgcctgc ggggggtcag
     7321 ctccatgcgg gtaacgggat tgatgacggg ttcgccgcgc ttgagtgcgc ccaaatcgcg
     7381 ggccagttcg ggtaggcgga tatcgttgac gtccgctccg gcacggatcc attgagcgag
     7441 acggtccatc ggttcctgtg tcgcccgttc gtagtgatcg tagtggatga cgacggcgtc
     7501 atccagccgt tccgccaacg cccggaccag agaggtcttc cccgctccgg ccggtcccgc
     7561 gatgccgata acgtgtcttt ccgtgctgac catggaccgc ctcgattcct aagattcggt
     7621 tcgccgcaag agtttcctcg cgggtcgaac cgattataag gcaattcttg actgagttca
     7681 aatactttcg gcggtgccgt ccacacggcg acggcatccg gcgtcgagtc gacgcttcca
     7741 gcgtgcctgg ggagcggtcg gtcggatgaa tcagcattcc gttgacaggc ccagttcaat
     7801 ggtatatatc agcacgcaaa cgcttaaatt atcaggaaaa gggcaggcgc ccggaaggag
     7861 gaaacatggt ccagaggaaa gacgccgaca tcgagaagaa ggccctgggt gagggggcgg
     7921 aggacgatca ggataacgcc aaagcgacca tccagtggga tgtttcaaag atggagtcgt
     7981 catatgccaa cgtgtgcaat gtatcctgca cgcgcgaaga attcaccctt ctgttcggga
     8041 tgaacaagac ctgggatgcc ggccagcgaa agctgacggt tgacatgacg gatcgcgtca
     8101 tcgtcaaccc gtacgccgcg aaacggctct acctgctcct cggcaacgtc atccgccagt
     8161 acgaagaccg tttcggtgag atccacctgg acgtcgccga agagaaatga ttcagcgcgt
     8221 ccgatgatcg tgcaacaccg cgccgtatct gaatttatgc agatgcggcg cggacggcgg
     8281 cgcggaattt cgaaaacgat caagaaattt cacaaagggg cgttgaaacc ccttcccttg
     8341 gagaaggggg ccgttggcgt gtttccccca ctcgatcgga agaacggcgg ttcggttctc
     8401 aatgcgcccg gattccgcta ttctgttttt cgcggatgcg gcgcatgcgg aaatctcgcg
     8461 atccggcgcg tccttcaacc tcgggctgat tacccgaccc accgagcgca tctgcggcgc
     8521 ggcccctccg acatggtttt tgcgggaatg ccgtcaggta gggtaccgat ccgtcaagcg
     8581 cggagacgat tcaccgatcg aatccttgcc caagtgagaa aagatccgat tcggtccgcg
     8641 aacggcggac ccctttaaga gacggaagag atggacatcg ccaaggcgtg ggaaggtttg
     8701 cagtccgttc aggatcgcga tgaattcgtc gcggcatggc tgacggttca gtgttccatg
     8761 atccgggggt gcattcaagg cgtgctcgtt ctcatggact cagcggcggg gacgtacatg
     8821 cccgtcgctt catggccgga gaagggctcc ttcgagcggc tctcggatat cctgaaccgg
     8881 acgctttccg aaaaagaggg aatgcttgtc gagctgaacc cctccgacgc aatacccgac
     8941 gaggacgacc ggcggtacgc cttggcctat ccgatcgttg ttgacgagct tctcatcgga
     9001 tcggttgccg tcgaggtagc ggcgtcttcg gacgcgtctc tgcgcagggc catggaggag
     9061 cttcagtggg gcgcggtatg gcttgagaac ctgaatcgcc gcgagcaggg catcgaagac
     9121 cgggatacac tgaaacgcct gaaggcggcg gtggacattc tcgccgttgt gctggcggag
     9181 ggaagcttcc aggaggccgc cacggcgttt gtcacggagg tggccacgag actgtcctgc
     9241 gaccgggtca gtttcggact ggtcaagggg agttcggttc gcgtcaaggc catttcccat
     9301 accgcaatcg tcggcaagaa gatgaacctc gtgcgctcca tcggggccgt catggaggag
     9361 gccgttatgc aacggacgga gattgtgtat cctccgcccc cggatgcgga gccggcggtc
     9421 gtccgcgatc atgagcgcat gatccaggtc catggcgacc gttcgatgct gactcttccc
     9481 ctgtatgcgc atgaccggta ctacgccgcc ctgacgctgg aaagacagga cgaccgaccc
     9541 ttcacgaagg acgaacagtc gtacatcaag agcgtggcgg ccctgaccgg tccggccctg
     9601 gagaacaaat tccagaacga ccgtgcgatc gtcttcgtca tcctcgcctc gctcaagcgg
     9661 caggccgaaa agatcttcgg agccggatat gtgggccgga aggtcgcgat cctggcagcc
     9721 gtgctgctga tcgtcttctt ttccttggcc aggcaggact accgcctgac cgcggatgcc
     9781 gttctcgagg gatcggtcac ccgctccatc gtcgttccga tggacggcta tatccaggag
     9841 agcttcgtca aggcgggcca cacgatcccg aaagacgccg tgatgtgcag aatggacggg
     9901 cgagacctcg aactggagag acaaaactgg cttggccgca agcggcagta cgaacgtcaa
     9961 tatcaggaag ccgttgcaaa acataaccgg gccgaggcca gcatcatcag cgctcagctc
    10021 gaccaggcgg aggcccagat cgcgctggtg gacaaaaagc ggtcacagat cgtaatccgc
    10081 gcgcccttca cggggatcgt tatcaggggc gatctcagtc agcggctggg cggcaccgta
    10141 gcgaaggggg aggagatctt ccaaatggcc cccctcgacg cctaccgttt gatcctgaag
    10201 gtggacgaac agcgcatcac ggacgtcagc gaggggaaga agggcgtcct ggttctctcc
    10261 tccatggccg gccatccctt cggattcacc gtccgaaaga ttacgccgat caccaccgct
    10321 gccgaaggcc gtaactattt tcgcgtggag gcggacctcg aacaggcgtc tccacggctt
    10381 agacctggga tggagggggt tggaaagatc gacgtggacc gaaggcggat catctccatc
    10441 tggacgcgag acttgatcga ctggttccga ctcgagctct ggtcctggct cccttagagg
    10501 gtgttcatgt cggaaaagac cctgtacagc aattcctggt atcgcgttgc ggatctcaag
    10561 cctttcgtca gaaatcatgc ggagatccac cgtcaagatt accgggggca ggcatggtat
    10621 gtccttgagg accacgccag cggtcgattt cacaggttta cggaagagac gtacttcatc
    10681 atcggcctga tgaacggcga gcggacgctc caggaaatat gggaggccgc ctgcaacaag
    10741 ctgggtgagg acatgccgac ccaggacgag atgatcctgc tggtttccca actgcaccag
    10801 gcggatgtgc tccaggccga tattgcccca gatatcgaga atcttcaccg caggcagcag
    10861 aaggaccgaa agactcgctt ctggaatgcc gtccgatcgc cgctggctct gcgactcccc
    10921 atcctggatc cggaccggtt tctcgaaaaa accgtaggcc tggtgcgccc cttctacagc
    10981 ctgccggcgg cattcgtctg gctcgtcatc gtcacgtcgg ccgtcgtgct ggccgtgacc
    11041 cattggcagg aactgacctc gaaccttgcg gatcgcgtcc tgcttctgga aaacatcctc
    11101 gtcttctgga tgatttatcc catcatcaag gtgtttcatg aattcggcca tgcctatgcc
    11161 gtgaaacgat gggggggaga agttcacgaa atgggcgtga tgttcattgt cttcatgcct
    11221 atcccttacg tcgacgcctc gtcctcgacg gcgttccggg accggcggaa gcgcatcgtc
    11281 gtcggcgctt cgggcatcgc cgtcgagctg ctcatcgcgt ccttggcgat gatcctgtgg
    11341 ctgaacctcg aagcgggcgc cgcaagggcc attgcttaca atacgatgat catcaccggt
    11401 gtttcgaccc tgttcttcaa cgggaaccca ctgctgaaat tcgacgctta ttacgtcctt
    11461 tgcgatctga tcgagatccc gaatctggga acgcgcgcca atcg
//
LOCUS       DAOOLJ010000061        11329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_6425_length_11329_cov_15.373337, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000061 DAOOLJ010000000
VERSION     DAOOLJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_11329_cov_15.373337"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..875)
                     /locus_tag="PLW16_08540"
     CDS             complement(90..875)
                     /locus_tag="PLW16_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB70994.1"
                     /translation="MERKFVNYVVEDRLAVVTIDNPKMNALSVPVVEELGAVFDELET
                     RTGIGVAIIIGGGTRAFVAGADIKGFKDLLGRRDFVFNGAREMQNVFTKIERSRLVVI
                     AAINGLALGGGCELAAACDIRIAGDNVIIGVPEVKLGLIPGAGGTQRLARLLGKGKAK
                     LMVLSGDFFSAQDACQMGLVEKVVPVADVLAEAKKLAGTILGNAPLAVEAGKRAINEG
                     VEMSLDDGLVFEAGFVGDLFMTEDLKEGAAAFVEKRFPVFKRK"
     gene            complement(1058..1855)
                     /locus_tag="PLW16_08545"
     CDS             complement(1058..1855)
                     /locus_tag="PLW16_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB70995.1"
                     /translation="MTTEGMRVLAFNGSPRDRRGNTEKILQPFLEGAAAAGAVTETLY
                     LKELKIRECQGCYACHMLTPGRCAIRDDMTWVLEKMLASDVLVFASPLYVFTVSAYLK
                     TLLDRMMILGTLEVKVVDGVVVHPPRFPERKWRWVVISNAGFPERELFEPMEGVFRRF
                     AWAIGGGTQVTIAGMILKGMGELFSVPSMLPNYQWFFDACRQAGGEVVREGAIAPATQ
                     AVLDRPLLDVDPEAFAAMSNTFIDLAAKKARSRKTGQEKTQGTAVSG"
     gene            complement(1855..2991)
                     /locus_tag="PLW16_08550"
     CDS             complement(1855..2991)
                     /locus_tag="PLW16_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70996.1"
                     /translation="MYFNETHEAFRASVKRFVDREINPNMEAWEAEGVPLHELFRKMG
                     DLGFLGIRYDPRWGGQGLDYWFETVFIEELGHVWGWGLPVAIMVQTNMATPAIDMFGS
                     DFLKERYLKPAIAGEKVAAIAVTEPGAGSDVAALSTRAVRDGDAYVINGSKTFITNGC
                     QADFLTLLARTSNDPGHHSYGLFVVPTDLHGFSVSKKLDKVGLRSSDTAELFFDNVRV
                     PAVNLIGNEREGFIYQMQQFQHERFTILPITYIACRDIIDMTVTYIKQRIVFGKPLST
                     KQVLRHRLVEWLAEIESLRHLTYHVVRMKTAGLDATREISMGKLLAAQLLNKVASGCL
                     QMFGGMGFMNETLISRHFRDTRVMSIAGGADEVMKDIVARMEGF"
     gene            complement(3055..3825)
                     /locus_tag="PLW16_08555"
     CDS             complement(3055..3825)
                     /locus_tag="PLW16_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB70997.1"
                     /translation="MKESTVLIGKQDRICTLTINRPYIKNAWTLEFVEELRDAFLAVG
                     RDKEIHCVVLEGAGRDFSSGADYALFKAALSPSVWLDGMRTLKALIMAMRGIPQPVIA
                     KVRGVAIGGGANLALAADFVIAAEDARICENFSNIGIIVDAGGNYFLPRLVGLAKARE
                     LLYLGDMLSGRAAADMGLIYKAVPDAELDAAVDALAQRLAGKSLEVMALIKEALDTSF
                     EMSLQTTMEWEAAHQSIMTQREELKTVVELYLKSRGKA"
     gene            complement(3844..4992)
                     /locus_tag="PLW16_08560"
     CDS             complement(3844..4992)
                     /locus_tag="PLW16_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB70998.1"
                     /translation="MKYDVFNEEHEQFRVQLRRFLADRVTPHAAEWERNRQIPRQIWQ
                     EMGQLGFLGFCYDPAYGGVGADDLFRVVMSEEMARCSAGGFGVAVCAHNDMSTTYIDL
                     LGTHEQKLRWLKPCTAGEKVCAIAVSEPGAGSDVAGIATRAERKGDHYVIEGQKTFIT
                     NGYYGDILVTAVKTDTKAVPPHKGISLIVVEKGMKGVTARKLEKIGGHASDTAEIFFD
                     QVEVPVEHLLGAEGQAFYAIMKNFQLERLVATILAVSFAQHILELTMDHAKQRVAFGK
                     PIAKFQAVRHKLAEMATAIELNRALSYQCAVQYAKGEDVTKEISMLKYASAEMVNRVA
                     YDATQLFGGYGYMAEYEVARLYADLRAVTIAGGTTEIMKDIVGRLMGL"
     gene            complement(5036..6589)
                     /locus_tag="PLW16_08565"
     CDS             complement(5036..6589)
                     /locus_tag="PLW16_08565"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HPB70999.1"
                     /translation="MRIGDLLTRAAKWYPEKEAVVFEGTRLTYRQLNERVNALAHALI
                     DMGVRKGDRVALVCHNSHYYAEVVFALAKIGAVSTNLNWRLSPREMAFLVDDSDAQVV
                     IFSQRFAYLYEPMRVLLKREFRFVGVEGTLGEGMIDYDELIARYPNHEPEVEVDEDDV
                     FLQLYTSGTTGRPKGVMLTHANMIANAHSTILEMEMQRDWNILGLLPVFHIAIFILID
                     LVMVGAKVTFVHTFDPAVLLATIEKEKVNTLGFTPVIFSFLLAHPDLNRYDLSCVKNI
                     IYATAPMPVDLLVRSIEKFQCRFIQFFGMTEMSPTLTMLIPEDHVMEGPEHKVRWLGS
                     AGRPVMNVDVKIVDEQGRDCPPGVTGEIIARGANMMKGYHKLPDETAAAIRDGWYHTG
                     DMGFFDAYGYLFIADRKTDMIISGGENIYPREVEEAILKLDGVQEVAVIGIPDDAWGE
                     AVRAIVVKRPGAELTEAQVIEHCRQHIASYKKPKGVDFVEALPRSAVGKVLKHELRAP
                     FWKGRTRKV"
     gene            complement(6653..7642)
                     /locus_tag="PLW16_08570"
     CDS             complement(6653..7642)
                     /locus_tag="PLW16_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB71000.1"
                     /translation="MLKTRITELLGIQHPVLMGAMHRVTTAEMVAAVAEAGGIGFIPA
                     AAFASLDALRGEIRKARTLTDGPIGLNISLVPAVHPGEKRIREIIEVGVEEGVGAFET
                     AGGSPAAFLPLIKASRIPVLHKVTQVRYARKAEEIGADAVIVIGCEGGGYIGPAEIPT
                     MILVEGAARALSIPVVAAGGIADGRTMAAALTLGAEGVLMGTRFIASTEAGLHARYQE
                     RLVETGETDTVVLFRSFGSPLRFIRNRLTPEVERMEREGRSMREILDRIGPGQEKDLR
                     LDDDPQGRLFPVGFGVTLIDRIRPIAEIVAEIVREAETAILCLDRVKGETAAA"
     gene            complement(7650..8456)
                     /locus_tag="PLW16_08575"
     CDS             complement(7650..8456)
                     /locus_tag="PLW16_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB71001.1"
                     /translation="MGFEHLFKVEQEGHIAWLILNRPEKRNAIGFDFFRGIMAHFQRF
                     DEDPSVRVVIMKGEGKCFTAGTDVMELAELIGDGSAYSRELQREKILLGQECFNAIEK
                     CRKPSIAAVHNYCVGVGVDLLSACDIRIATRDALFGVRETRMGVVADVGTLQRLPHII
                     GHGWFRELALTGRDFGAEEALRMGFITRICEDREALYGEARRLASEVLACSPLAVYGA
                     KDVILYSRDHGVYPGLKYVAQKNAAAIPSEDMLEAMQAFMEKRPPVFKGK"
     gene            complement(8618..9427)
                     /locus_tag="PLW16_08580"
     CDS             complement(8618..9427)
                     /locus_tag="PLW16_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB71002.1"
                     /translation="MMGNESVFKLEREGHIVWLTLNRPEKRNAMGRAFYRGMADFCRQ
                     FDQDPDVRVVVLKAEGKSFTTGTDMIELGELVGDGSALSRDLLRDQILWGQGCISEIE
                     ACRKPVIAAAHGLCLGGGVDLLSACDIRIATRDVVFGIRETRVGVIADVGTLQRLPHI
                     IGHGWFRELALTGRDFGAEEALKMGFITRICADREALYEEARRLALEVVACSPLAVQG
                     AKDVILYSRDHGVYPGLKYVAQKNAAAIPSEDMLEAMQAFLEKRAPVFKGK"
     gene            complement(9548..11251)
                     /locus_tag="PLW16_08585"
     CDS             complement(9548..11251)
                     /locus_tag="PLW16_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71003.1"
                     /translation="MTYNDRPWVRFYNEWVHPEVSIPEKTYLDYLNESFRRNPHRPAL
                     YFMGKEIVFGELEDLSARFANFLAEIGCVPGTPVSVITPNIPQNQIANVGICRAGCVA
                     QGISLLLTPTEMAYQLNHSGARVLIILDLAFEQKFVKIQQEVPNLTHVVVCRIGDYLP
                     AVKRVLGGILGKLPTGQVTPIAGKHVLTFSEVMRNHPAQAPDVRLSPEDTCLIQYTGG
                     TTGIPKGAEVTHRNIVSCLVQMKNWLDLKEMEEVYCSAFPFFHIAGLFVGMINLSMGN
                     PQVLIPDPRNIEHFCKAYVKHRPTGVANVPVIYQQLAEEPAFRAADHSRCKAFISAAS
                     PLPEKLYRRLEATVGQGKVLELFGMTETMVTSLDAPGKKKKIGSVGLPWPAYRLKIVD
                     LESGTREMPIGQEGELIIRGPGVMKGYWRMPEETAHALRELDGEKWLYTGDVVRMDDD
                     GYLYVVDRCKDMIDVRGYNVFSREVEDRLYQHPSVEFCAVVGVPDPERPGTQQVKAVI
                     QLKAEFRKNDPKALQSEILEHCRETLAHYKVPQAIEFVESIPLTSVGKVDKKVLRPKA
                     S"
BASE COUNT         2053 a         3829 c         3204 g         2243 t
ORIGIN      
        1 caaaacaatg ggatatcgta tcccgaacag ttcggtaatg cgtgtcttca tggaatgact
       61 ctcctgtgat cctgcggact cgcccgcggt tatttgcgtt tgaagacggg gaagcgcttc
      121 tccacgaagg cggcggctcc ttccttgagg tcctcggtca tgaacaggtc ccccacgaat
      181 ccggcctcga acaccaggcc gtcgtcgagg gacatttcca cgccctcatt gatggcccgt
      241 tttccggcct ccacggccag gggggcattg cccaaaatcg taccggccag ctttttcgcc
      301 tccgccagaa cgtccgccac cggcacgacc ttctccacga gtcccatctg gcaggcgtcc
      361 tgggcgctga agaagtcccc gctcaggacc atcagcttgg ccttcccctt gcccaggagt
      421 cgggccaggc gctgcgtccc gcccgcaccg ggaatcaggc cgagcttgac ttccggaacg
      481 ccgatgatga cgttgtcccc ggcaatccgg atatcgcagg ccgccgccaa ctcgcagccg
      541 ccccccaggg ccaggccgtt gatggcggcg atgacgacca gccgtgaacg ctcgatcttc
      601 gtgaagacgt tctgcatttc gcgagcgccg ttgaaaacga agtcccgtct tcccaggagg
      661 tccttgaatc ctttgatatc cgctcccgcg acgaacgccc gggttccgcc gccgatgatg
      721 atggccacgc cgatccccgt gcgggtttcc agctcgtcga acaccgcgcc gagctcctcg
      781 acgaccggaa cactcagggc gttcatcttc ggattgtcga tggtcaccac cgccagacga
      841 tcctccacga catagttcac aaattttctt tccacggttt gactcctcct ttaagttttt
      901 tcaggaatcc atagaatccc cgaaagtagc tccgatgaat cgaggtgaaa cgcaccccga
      961 gggtttatcg cggctttcca ccgccatctt tttctaaggt gtaccgttgc tgttttctgg
     1021 aaggataggc tcccatagca caggccggcc ggggaattca accggatacc gccgttcctt
     1081 gcgtcttttc ttgccccgtc ttgcgggaac gggccttttt cgcggcgagg tcgatgaagg
     1141 tgttgctcat ggccgcaaac gcctcgggat cgacgtcgag cagcggccgg tcgagcacgg
     1201 cctgcgtggc gggcgcgatc gccccttccc ggaccacctc tccgcccgcc tgccgacagg
     1261 cgtcgaagaa ccactgataa ttcggcagca tcgacggcac cgagaagagt tcgcccatcc
     1321 ccttgaggat cataccggcg atcgtcacct gcgtcccgcc cccgatagcc caggcgaacc
     1381 gccggaacac cccctccatc ggctcgaaga gttccctctc gggaaagccg gcgttggaga
     1441 tgacgaccca acgccatttc cgctcgggaa agcgcggggg atgcaccaca acgccgtcga
     1501 cgaccttcac ctcgagcgtc cccaggatca tcatccgatc gagcagcgtc ttgagatagg
     1561 cgctcaccgt aaagacgtag aggggcgacg cgaacacgag gacatcggac gccagcatct
     1621 tctcgaggac ccaagtcatg tcgtcccgga tggcgcaccg gccgggggtc agcatgtggc
     1681 aggcgtagca tccctggcat tcccggatct tcagttcctt gaggtagagg gtctcggtca
     1741 ccgctccggc cgcggcggcg ccctccagga acggctggag gattttttcc gtattcccgc
     1801 gccggtcgcg ggggctgccg ttgaaggcca ggacccgcat gccttcggtc gtcatcagaa
     1861 cccctccatg cgggcaacga tgtccttcat cacctcgtcc gccccgccgg cgatgctcat
     1921 cacccgcgtg tcccggaaat gccgggaaat gagcgtctcg ttcatgaacc ccatgccgcc
     1981 gaacatctgc agacacccag aagcaacctt gttcagcagc tgcgccgcga gcagtttccc
     2041 catggagatc tcccgcgtgg cgtcgagccc cgccgtcttc atccgcacga cgtgataggt
     2101 cagatgcctc aggctctcga tctcggcgag ccactccacc agccgatggc gcagcacctg
     2161 cttcgtgcta agtggcttcc cgaacacgat ccgctgcttg atgtaggtca ccgtcatgtc
     2221 gatgatgtcc cggcaggcga tgtacgtgat gggtaggatc gtgaagcgtt cgtgctgaaa
     2281 ctgctgcatt tggtagatga acccctcccg ctcattgccg atcaggttca ccgccggcac
     2341 gcggacgttg tcgaagaaca gctccgccgt gtcgctgctc cgcaggccga ccttgtcgag
     2401 ctttttgctc acgctgaacc cgtgcaggtc cgtgggcacg acgaacaggc cgtaggaatg
     2461 gtgcccgggg tcgttcgacg tccgggccag gagcgtcagg aaatcggcct ggcagccgtt
     2521 cgtgatgaac gtcttcgacc cgttgatcac gtaggcgtcc ccgtcccgga cggcccgggt
     2581 cgacaatgcg gcgacatcgg acccggcacc cggctccgtc accgcgatcg ccgccacctt
     2641 ctcgcccgcg atggccggct tcaggtacct ctccttgagg aaatcgctcc cgaacatgtc
     2701 gatcgccggg gtggccatgt tcgtctgcac catgattgcc accgggaggc cccagcccca
     2761 gacgtggccc aactcctcga tgaagacggt ctcgaaccag tagtccaggc cctggccgcc
     2821 ccaccggggg tcgtagcgga tccccaggaa ccccaggtcc cccattttac ggaacagctc
     2881 gtgcagcggc acgccctccg cctcccacgc ctccatgttc gggttgatct cccgatccac
     2941 gaagcgcttc accgaggcgc ggaacgcctc gtgtgtctca ttgaaataca tgtttcccct
     3001 ccacgatcac ctttttgccg ttcgcttgca ctccccgcca ccccgctgat ccgttcaggc
     3061 cttaccgcgg gacttcaggt acagttccac gacagtcttc aattcctccc gctgcgtcat
     3121 gatcgactga tgggccgcct cccactccat cgtcgtctga agcgacattt cgaaacttgt
     3181 gtccagggcc tccttgatca gggccatcac ctcgagcgac tttcccgcca gcctctgcgc
     3241 cagtgcgtcg accgcggcat ccagctcggc atcgggcacg gccttgtaga tgaggcccat
     3301 gtcggcggcc gcacggccgc tcagcatgtc gccgagataa agcagttccc gggccttcgc
     3361 cagacccacg agccgcggca ggaaataatt ccccccggca tcgacgatga tcccgatgtt
     3421 cgagaagttt tcgcaaatcc gcgcgtcctc ggcggcaatg acaaaatccg cggcgagggc
     3481 aaggttcgcc ccgcctccga tggccacccc ccgcaccttg gcgataaccg gctgagggat
     3541 gccccgcatg gccatgatca acgccttgag cgtccgcatc ccatccagcc agacggaggg
     3601 agacaaggcc gccttgaaga gcgcataatc ggccccggag gaaaaatccc gtcctgcccc
     3661 ttccaggacg acgcaatgga tctccttgtc acgtcccacc gccaggaagg catcccgcaa
     3721 ctcctccacg aattccagcg tccaggcatt cttgatgtag ggccggttga tcgtcagggt
     3781 gcagatccgg tcctgcttgc cgatcaaaac ggtggattcc ttcatctcat cctccttttc
     3841 acgttaaaga cccatgaggc ggccgacgat gtccttcatg atctccgtcg tccccccggc
     3901 gatggtgacg gcccgcaggt ccgcatatag gcgggccacc tcgtattcgg ccatataccc
     3961 gtacccgccg aacagttggg tggcgtcgta ggcgacccgg ttgaccattt cggccgaggc
     4021 gtatttgagc atggaaattt ccttggtcac gtcctcgccc ttcgcgtatt gaacggcgca
     4081 ttgatagctc agggcgcggt taagttcgat cgccgtggcc atttccgcca gtttgtggcg
     4141 caccgcctgg aacttcgcga tcggcttgcc gaaggccacc cgctgtttcg cgtggtccat
     4201 cgtcaactcc aggatgtgct gggcaaacga cacggcgagg atggtggcga ccaggcgttc
     4261 gagctggaaa tttttcatga tggcgtagaa ggcctgtccc tcggcgccga ggaggtgctc
     4321 cacgggcact tccacctggt cgaagaagat ctccgccgta tccgaggcat ggccgccgat
     4381 cttctccagc ttccgtgcgg tcaccccttt catgcctttc tcgacgacga tcagcgagat
     4441 gcccttgtgc ggcggaaccg ccttcgtgtc ggtctttacg gcggtgacca ggatgtctcc
     4501 gtaatacccg ttggtgataa aggtcttctg gccctcgatc acgtaatgat cgcccttgcg
     4561 ctccgcccgc gtggcgattc ccgccacgtc ggagcccgcg ccgggctccg acacggcgat
     4621 ggcgcagacc ttctcgccgg cggtgcaggg cttgagccac cgcagcttct gttcgtgcgt
     4681 gcccaagagg tcgatgtagg tcgtggacat gtcgttgtgc gcgcaaaccg ccacgccgaa
     4741 ccctcccgcg gaacaccggg ccatctcctc ggacatgacc acccggaaga gatcgtccgc
     4801 gccgacaccg ccataggcgg ggtcatagca gaatcccagg aatccaagct gccccatttc
     4861 ctgccagatc tgccgcggga tctgccgatt ccgttcccac tcggcggcat ggggggtgac
     4921 cctgtccgcc aaaaatctcc ggagttgcac ccgaaactgc tcatgctctt cgttgaagac
     4981 atcgtacttc atgctgatcg ttcctcctta ttccctttcg tttccggcaa ccgcttcaga
     5041 cctttcgggt ccttcccttc cagaagggcg cacggagttc gtgtttcaga accttgccca
     5101 cggcgctccg cggcagcgcc tcgacaaaat cgaccccctt cggcttcttg tagctggcga
     5161 tatgctgccg gcaatgctcg atcacctgcg cttccgtcaa ctccgcgccc ggcctcttga
     5221 cgacgatggc gcggaccgcc tcgccccagg cgtcgtccgg aatgccgatg acggccactt
     5281 cctggactcc gtccagtttg aggatcgcct cctccacttc ccggggatag atgttttccc
     5341 cgccggaaat gatcatgtcg gttttccggt cggcgatgaa aaggtagccg tacgcgtcga
     5401 agaagcccat gtcgcccgta tggtaccagc cgtcccggat ggcggccgcc gtctcgtcgg
     5461 gcagcttgtg ataccccttc atcatgttgg ccccgcgggc gatgatctcg cccgtgacgc
     5521 ccgggggaca gtcgcggccc tgctcgtcga cgatcttcac gtcgacgttc atgacgggac
     5581 gtcccgccga tcccagccag cgcaccttgt gttccggccc ctccatgacg tgatcctcgg
     5641 ggatcagcat ggtcagcgtc gggctcattt ccgtcatgcc gaagaattgg atgaaccggc
     5701 attgaaactt ctcgatggaa cggacgagaa gatcgaccgg catcggcgcg gttgcgtaaa
     5761 tgatgttctt cacgcagctc aggtcgtacc ggttgaggtc ggggtgcgcc agaaggaagc
     5821 tgaaaatcac gggcgtgaag cccagggtgt tgaccttttc cttttcgatc gtcgcgagca
     5881 gaacggccgg atcgaaggtg tggacgaagg tcaccttggc gcccaccatg acgagatcga
     5941 tcaggatgaa gatggcgatg tgaaagacgg gcagcagccc caggatgttc cagtcccgtt
     6001 gcatctccat ctccaggatc gtgctgtggg cattcgcgat catgttcgcg tgggtcagca
     6061 tcaccccctt ggggcggccc gtggtcccgc tggtatagag ctgcagaaac acgtcgtctt
     6121 catccacctc gacttccggc tcgtggttcg gataccgggc gataagttcg tcgtaatcga
     6181 tcatgccctc tcccagcgtt ccctcgacgc cgacgaagcg gaattcgcgc ttcagcaata
     6241 cgcgcatggg ttcgtacaga taggcgaacc gctgggagaa gatcaccacc tgcgcgtccg
     6301 aatcatccac gagaaaggcc atctcccggg gggaaaggcg ccagttgagg ttcgtcgaga
     6361 cggccccgat cttcgccagg gcgaaaacga cctcggcgta gtaatgggaa ttatggcaga
     6421 caagcgccac ccggtccccc ttcctcactc ccatgtcgat taatgcatgg gcgagggcgt
     6481 tgacccgctc gttcaattgc cggtacgtca ggcgcgtgcc ctcgaagacc accgcctctt
     6541 tttccgggta ccatttcgcc gcccgggtca gcaaatcacc aatccgcatc gatggtccct
     6601 ccttctcctg agcgatcctt ccgtccagtc tatcaccgga gcggtggacg ggctatgccg
     6661 ccgccgtctc cccctttacc cgatccaggc acaggatggc cgtctccgcc tcacggacga
     6721 tctccgccac gatctccgcg atgggccgga tgcggtcgat cagcgtcacg ccgaagccga
     6781 cgggaaacag ccgtccctgc ggatcgtcgt ccaggcgcag gtccttctcc tgacccgggc
     6841 cgatcctgtc gagaatctcc ctcatcgagc gcccttcccg ctccatgcgc tcgacctcgg
     6901 gggtcagccg attgcggatg aagcggaggg gactgccgaa cgaccggaag aggacgaccg
     6961 tatccgtctc gccggtttca acgaggcgtt cctgataccg ggcatggagc cccgcctccg
     7021 tcgaggcgat gaagcgggtc cccatgagca ccccctccgc cccgagggtg agcgcggcag
     7081 ccatcgtccg tccgtcggcg atgccgcccg cggcgacgac cgggatggaa agggcgcggg
     7141 cggcgccctc caccaggatc atcgtcggga tctccgccgg tccgatatag ccgcctcctt
     7201 cgcaaccgat gacgatcacg gcatcggcgc cgatctcctc cgccttccgg gcatacctga
     7261 cctgggtcac cttgtgcagg acagggatgc gcgatgcctt gatcagcggg agaaaggcgg
     7321 cgggggagcc ccccgccgtc tcgaacgccc ccaccccctc ttcgacgccg acctcgatga
     7381 tttcccggat ccgcttctcc cccgggtgga cggcgggcac cagggagatg ttgaggccga
     7441 tcggcccatc cgtgagggtc cgggcctttc ggatctcacc ccgcaaggca tcgagggacg
     7501 cgaaggcggc ggccggaatg aaaccgattc ctcccgcctc ggccacggcg gcgaccatct
     7561 cggcggtggt gacccgatgc atggcgccca tcaaaacagg gtgttggata ccgagaagtt
     7621 ccgtgatgcg cgtcttcagc ataaggttct cacttcccct tgaaaacggg cggtcgtttc
     7681 tccatgaagg cttgcatggc ttccagcatg tcctcggacg gaatggccgc ggcattcttc
     7741 tgcgccacgt acttgaggcc cggatagacg ccgtgatccc ggctgtagag aatcacgtcc
     7801 ttggccccgt aaacggccag tggggaacag gcgagcactt ccgaggcaag ccgccgcgcc
     7861 tccccgtaga gggcctcccg gtcctcgcag atccgcgtga tgaatcccat ccgcagggcc
     7921 tcctccgcgc cgaaatcccg gccggtcagc gccagttccc ggaaccagcc gtgaccgatg
     7981 atatggggaa gccgctggag cgtccccacg tcggccacga cgcccatccg ggtttcacgc
     8041 acgccgaaga gggcgtcgcg ggtcgcgatc cggatgtcgc aggccgacag cagatcgacc
     8101 ccgacgccga cacagtaatt gtggacggcg gcgatcgacg gcttgcggca tttttcgatc
     8161 gcgttgaagc actcctgtcc caggaggatc ttctcgcgct gcagttcgcg cgaatacgcc
     8221 gacccatcgc cgatcagctc cgccagttcc atgacgtccg tcccggcggt gaaacacttt
     8281 ccctctccct tcatgatgac gacccggacc gacggatcct cgtcgaaccg ctgaaaatgc
     8341 gccatgatgc ccctgaagaa atcgaacccg atcgcattgc gcttctccgg ccggttaagg
     8401 atgagccagg cgatgtgccc ctcctgttcg accttgaaca gatgttcaaa ccccatgttg
     8461 acgcctcctt cttcaaattg ccggaccgat ccggcccggt ctccctttca cgcgctgttt
     8521 cgttccgggc gacggtcgcc cgcgcctccc gtcccgccac gatgtcggac ctccgggcaa
     8581 cccggcggcg atccgccgcg ccgtggatcc tgcccgttca ctttcccttg aagacgggcg
     8641 ctctcttctc caggaaggcc tgcatggcct ccagcatgtc ctcggacgga atggccgcgg
     8701 cgttcttctg cgccacgtac ttgagtcccg gatagacgcc gtgatctcgg ctgtagagaa
     8761 tcacgtcctt ggccccctga acggccagag gagaacaggc gaccacctcg agcgcaagcc
     8821 gcctcgcctc ctcatagagg gcttcccggt ccgcgcagat ccgcgtgatg aaccccatct
     8881 tcagggcctc ctccgcgccg aaatcccgac cggtcagcgc cagttcccgg aaccagccgt
     8941 ggccgatgat gtgcggcagg cgctggagcg tccccacgtc ggcgatcacc ccgacccgcg
     9001 tctcccggat accgaagaca acgtcccggg tcgcgatccg gatgtcgcag gccgacaaca
     9061 gatcgacccc accgccgaga cagagcccgt gcgccgcggc gatcacgggt ttgcggcagg
     9121 cttcgatttc gctgatgcag ccctgccccc acaagatctg gtcccgaagc agatccctcg
     9181 acaatgccga tccgtcgccg accagttcgc ccagttcgat catgtccgtg ccggtggtga
     9241 agctcttgcc ctcggccttg aggacaacga cgcgcacatc cggatcctgg tcgaactgtc
     9301 ggcaaaaatc cgccatgccc cgatagaagg cgcgtcccat cgcgttgcgc ttctccggac
     9361 ggttcagcgt caaccacacg atatgtccct cccgttccag tttgaataca ctctcatttc
     9421 ccatcatcca tcctcccact cggccgatat ccaaaacccg tcacaggcgc tccggcccgg
     9481 accgcccccc tttgcaccct gttttcccca tcgtcgaacg gcttcaaaac gcggtccgcc
     9541 ggatccctca ggacgccttc ggccggagga ccttcttgtc gaccttgccc acggacgtca
     9601 agggaatgga ctcgacgaat tcgatcgcct gcggcacctt gtaatgggcg agcgtttccc
     9661 gacaatgctc caggatctcc gattgcaggg ctttcggatc gttcttccgg aattccgctt
     9721 tcaactggat caccgccttg acctgctgcg tccccggccg ttccggatcg ggaacgccta
     9781 caacggcgca gaactcaacg gacggatgct gatagagtct gtcctccact tcgcgcgaaa
     9841 agacgttgta gccccgaaca tcaatcatgt ccttgcaccg atcgaccacg taaaggtagc
     9901 cgtcgtcgtc catcctcacg acatccccgg tatagagcca tttctctcca tcgagttcgc
     9961 gcagggcgtg cgccgtctcc tccggcatcc gccaatagcc tttcatcacc cccggtcccc
    10021 ggatgatcaa ttccccctct tgcccgatgg gcatttcccg cgtaccgctt tccaaatcca
    10081 cgattttcag ccgataggcc ggccagggaa gccccacgga accgatcttt ttcttcttcc
    10141 ccggggcatc caggctcgtc accatggttt ccgtcatgcc gaacagctcc aggaccttgc
    10201 cttggccgac cgtcgcctcg aggcgacgat acagtttctc cggcaggggt gatgcggccg
    10261 agatgaaagc cttgcatcgg gaatggtcgg cggcccggaa cgccggttcc tccgccaatt
    10321 gctggtaaat caccgggaca ttggcaaccc cggtcgggcg atgcttgacg tacgctttgc
    10381 agaaatgctc gatgtttctc ggatcgggaa tgagcacctg cggattcccc atggagaggt
    10441 tgatcatgcc gacgaacaga ccggcgatgt gaaagaaagg aaaggcggaa caatacactt
    10501 cctccatctc cttgagatca agccagttct tcatttgaac cagacagctg acaatgttcc
    10561 ggtgggtgac ttcggccccc ttcggaatgc ccgttgttcc tcccgtatac tggatcagac
    10621 aggtgtcttc cggcgaaagc cggacatccg gcgcctgcgc cgggtggttg cgcatgactt
    10681 cggaaaacgt cagcacgtgt ttgccggcga tgggcgtcac ttgacctgtc ggaagtttcc
    10741 cgaggatccc tcccaaaacg cgcttcaccg ccggaagata atctccgatg cgacagacga
    10801 ccacgtgcgt caggttcggg acttcctgct gaattttgac aaatttctgt tcgaaagcca
    10861 gatccaggat gatcagcacc cgggcgccgg agtggttgag ctgatacgcc atctccgtcg
    10921 gcgtcagcag gagagaaatg ccttgcgcca cacaccccgc gcgacagatg cccacattgg
    10981 cgatctgatt ctgcggaata ttgggtgtga tgacggagac gggcgttccg ggaacgcaac
    11041 cgatctcggc cagaaaattg gcgaagcggg ccgatagatc ttccaattcc ccgaacacga
    11101 tttcctttcc catgaagtaa agggcgggcc gatgcggatt ccgcctgaag ctttcgttca
    11161 aataatccag ataggtcttc tccggaatgg agacctccgg atggacccat tcattgtaaa
    11221 accgcaccca gggtctgtcg ttgtacgtca taggacaccc cctaatgtcc ttcttgagaa
    11281 gcatgtccgg acttcaggat ttcgagtttc gtttctgtcg ttcttcttc
//
LOCUS       DAOOLJ010000062        11022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_6682_length_11022_cov_18.414881, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000062 DAOOLJ010000000
VERSION     DAOOLJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6682_length_11022_cov_18.414881"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLW16_08590"
     CDS             <1..206
                     /locus_tag="PLW16_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71004.1"
                     /translation="LIATARRGDTRLVAVVLGASTPGVRARETIRLLDEGFARMGVTP
                     PPARTRSDVARPPERDKIPTARP"
     gene            complement(229..1563)
                     /gene="rimO"
                     /locus_tag="PLW16_08595"
     CDS             complement(229..1563)
                     /gene="rimO"
                     /locus_tag="PLW16_08595"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPB71005.1"
                     /translation="MSETVHMISLGCPKNLIDSEVMAGLLAGEGFRLTAEAREAQIIL
                     VNTCAFILPAREEAIETILRMAALKTPAGGNCRYLIVAGCLPQRYGAALARALPEVDL
                     FLGVNEVPAVAAHLRRLLAGQTGRRNRTGKPTFLMDAGLPRLISTPAATAYLKIAEGC
                     NNRCAYCVIPAIRGPYRSRQPGDILREAETLVGQGVRELIVTAQDTTAYGQDLPGRPA
                     LPGLLHDLASMKKLRWLRLLYTYPERLTDGLFEVIARESKLCKYIDMPIQHIDDELLA
                     AMHRRGNSALIRERIARARAMIPGVALRTSLIVGFPGETPSRFNRLHAFLAEARLDHV
                     GIFTYSREEGTPAENRPPEISEATKRRRRGTLMIRQAGISEEINRTLIGTRQEILIAG
                     PSANPEYPFVGRCHRQAPDIDGVTYVRGGRLRPGTFVTRTILAADTYDLYAG"
     gene            complement(1566..3797)
                     /locus_tag="PLW16_08600"
     CDS             complement(1566..3797)
                     /locus_tag="PLW16_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPB71006.1"
                     /translation="MTEEIQGNRMGEIRGVVCLAGALFLFLSLFSYHPLDPSFTHFTA
                     GDAPIHNLAGTVGSHTADTLARLFGFGVFWFPVVLVIAGLKSLLERAYRLTGVGIAGF
                     VGLICATTGLFAVTIPRVLLHGVDLPTGGLTGQLLARLLLGYFNLAGAYLILTALLIV
                     SLMVLVDFSLVVLARRLFQVIVGGVRLLSVAIPRKIEALRQWRSKPDKEKKTKKKAAP
                     VIAAGSAEPRRTAKPEQIPLPLVMPSPGQAFQLPPLTLLDPVQHRELRQKKETLVANS
                     RVLENKLADFGVEARVLEVRPGPVITMYELEPAPGVKIHRITNLTDDLALALKAPSIR
                     IIAPIPGKAAIGIEIPNPDRDEVHLRDVLDHEDFRTSPCRLPIALGVDIVGQPVITDL
                     IRMPHLLIAGTTGSGKSVSLNAMICSILFKAPPDEVKFLMIDPKRLELSAYEGIPHLL
                     HPVVVDPKKASQVLRWAVEEMERRYQIIGERGVKGIEAFNELVKRAAAEKKGSPARAA
                     SLPSDGDPSPEAEAPPPPAPLPYIVIVIDELADLMLVAQRNVEESLARLAQMARAAGI
                     HLILATQRPSVDVITGVIKANFPTRISFQVSSKVDSRTILDVQGAENLLGAGDMLFIP
                     PGTSKLNRIHGAYVSDAEIERIVFYVKQQGQPAYDESITQYRPDAPEGDKEGEALDEK
                     YDEAVELVTDLGQASISLVQRYLKVGYNRAARIIERMESEGVVGPSDGVKPRKVLARK
                     LPR"
     gene            complement(3794..4531)
                     /locus_tag="PLW16_08605"
     CDS             complement(3794..4531)
                     /locus_tag="PLW16_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB71007.1"
                     /translation="MIRSALLVILGVAITGFVSLFCIVFPYISPGENKIHKIANLWAR
                     ILLLISATKVDVIGGENVLTRSPQIFMANHASDFDILIVLAHIPGQFRWIAKKELFRI
                     PLFGKAMRNAGYIEIDRQHHERAMRSIDEAAEKIKEGKSVMTFPEGTRSRDGKIQAFK
                     QGMFHLAIKSGVPIVPISIIGASEIMPKRSLRVNPGTITMVIDKPIDVTGYTIETREA
                     LIQKVREVIVRNFDEQRAIRLQSPECR"
     gene            4794..5783
                     /gene="rfaE1"
                     /locus_tag="PLW16_08610"
     CDS             4794..5783
                     /gene="rfaE1"
                     /locus_tag="PLW16_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPB71008.1"
                     /translation="MAQILTRKRALEIMAGFPLARVLVIGDIMIDHFIWGKVVRISPE
                     APVPVVDVKADDLMLGGCANVLNNIFSMGGKACVTGVIGADVMGDKVREEFRKRHIST
                     QGLVAEPRRPTSLKTRIVAHQQQVVRFDRESRTPIREESVRHILSYLRNLRDSLGAVV
                     ISDYGKGVVTPALLDGVRTEISGRPIVVCVDPKQRDFNPYRGFDVLTPNHHEAAEAVG
                     VEMGNGAELVAVGKQLIERYDLKALLITRGEAGMSLFERRGRIVHTHLPTEAREVFDV
                     TGAGDTVIGVFALCVASGATFREAAVLANHAAGIVVGKVGTATASREELKRVL"
     gene            5780..6313
                     /locus_tag="PLW16_08615"
     CDS             5780..6313
                     /locus_tag="PLW16_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPB71009.1"
                     /translation="MSRLATAPVVKLIMSVLAPAPDLIGQAVGVVSDLYGEADFVSAL
                     FPFDYTDYYAGEMGAPLYRRFVAFERLMPPETLPDVKRLTCEAEEQWTEGGRRRVNID
                     PGYLAEAHLILATGKGYAHRPYLRDGVYADLTLVYQHQAFRPLAWTYPDYGSPDVRAL
                     FEKIRAKYLLQRKEARH"
     gene            6403..7284
                     /locus_tag="PLW16_08620"
     CDS             6403..7284
                     /locus_tag="PLW16_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPB71010.1"
                     /translation="MKSMTGYGRAEGMCGGKKLVVEIRSLNHRYIEVSLRLPPPFFPW
                     ELEIKKRVTERFSRGKIEVALRMDAQEGLEAAGRLTLNWPLLRQYHALLVELKQAFNI
                     EDPITLSMLAGLREVFITSENGAPAENLWPDVEPILAEAMSMLSSMRQREGEMLRQDM
                     VMRLQLIQGEMDAIRKRSPEVVLECRGRLAERVRELANNLEIDEGRLSVEVAIMADKM
                     DITEELVRFASHLNQFHELVLSPDAVGRKIDFLIQELNREINTIGSKSADTDIARRVI
                     DIKSELSKLREQVQNIE"
     gene            7307..7906
                     /gene="gmk"
                     /locus_tag="PLW16_08625"
     CDS             7307..7906
                     /gene="gmk"
                     /locus_tag="PLW16_08625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPB71011.1"
                     /translation="MLIVVSAPSGTGKTSLCREVLAMFPELRFSVSWTTRPPRPGERD
                     GQDYCFTSEAEFRRRIEQGEFVEWVENYGHLYGTSIRTVQSFLDQGHDLLLDVDPRGA
                     RAIKERFPSGVFIFLLPPSLAALEVRLSRRGESADVMAARLAKAKDEIREMIWYDYLI
                     VNEQLDEAVDCLRAVYMAETCRRERFYDRMKGDLQSLTG"
     gene            7924..8196
                     /gene="rpoZ"
                     /locus_tag="PLW16_08630"
     CDS             7924..8196
                     /gene="rpoZ"
                     /locus_tag="PLW16_08630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPB71012.1"
                     /translation="MARITVEDSLRTAKNRFALVILTAKRARQLLKGSQPLAERKNNR
                     EIVTALREIADAQVVFSHPEYLRGIKEEYRPVLDQTEFVGDEEYTE"
     gene            8177..8950
                     /gene="pyrF"
                     /locus_tag="PLW16_08635"
     CDS             8177..8950
                     /gene="pyrF"
                     /locus_tag="PLW16_08635"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPB71013.1"
                     /translation="MKNIRSDAPRERLIFALDVGEGLTEVLSWVKRLRDHVGCFKVGK
                     EAFTHLGPGLVEAIHQGGGRVFLDLKFHDIPNTVARAAEAAVRMGVNMFNVHALGGSR
                     MMVETATAVRNMAARLDVPAPVILAVTVLTSLDDRDLSDLGFRMSTEALVVHLAVQAR
                     AAGLDGVVASARDVPAIRAACGEACLIVTPGIRRDGVDAGDDQKRILSPEGAVAAGAD
                     YLVVGRPIRMAVDPAAEADDIVRAITAGLATRTGAPPRV"
     gene            8965..9726
                     /gene="rlmB"
                     /locus_tag="PLW16_08640"
     CDS             8965..9726
                     /gene="rlmB"
                     /locus_tag="PLW16_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPB71014.1"
                     /translation="MRASNGGCQEILYGINPVREALRHGGVDIREIWVTDGRGGAPLT
                     EILEQARQRGIPVAYRRREELDRRSGVRSHQGLVAVGRAFAYADLEEVVANRHEAFRH
                     GCLLILDGVLDPQNLGSLIRTAHCFGVNGVIIPENRAAAVSPAAVKASAGAAGHIPVA
                     RVVNLVQTIQLLKDRGFWIYGADTRGGRQPEELVIEGDIALVMGSEGKGIRPLVRRQL
                     DFPLSIPLVGCLDSLNVSVAAGIILHAIMKQRTRQ"
     gene            9760..10971
                     /locus_tag="PLW16_08645"
     CDS             9760..10971
                     /locus_tag="PLW16_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB71015.1"
                     /translation="MPAFLWEATSRKGEQKKGELDAADEATVRAQLRRQGFKNISVKA
                     KPKDLMEYLPFLQQSVKEKDVVVFSRIFSTMINAGLPLIQCLELLASQEQNKTFAGII
                     RAIKEDIEGGSTLTDALKKYPKIFDELFVNLVAAGEAGGILDVILSRLSAYMEKAMKL
                     KSKVKGAMTYPASVLVISIGVVAVLLLKVIPVFQKMFEGMGGTLPAPTQFLVNASQFA
                     QNYFYVFIIVIVAVVFAYKRFVATEKGRYVVDSLALKAPVFGQLIRKVAVSKFTRTMS
                     TMMSSGVPILEALNIVSKTAGNVIIENALIQTRKSISEGRTIAEPLMETDIFPPMVVQ
                     MIAVGEATGALDTMLAKIADFYDDEVDAAVDALTSLLEPFMMVFLGGVVGGMIIAMYL
                     PIFQMASVVGG"
BASE COUNT         2003 a         3211 c         3779 g         2029 t
ORIGIN      
        1 acctcattgc gacggcccgc cggggggaca cccggctcgt ggccgttgtc ctcggcgcct
       61 cgaccccggg ggtccgggcg cgggaaacca tccgcctgct cgacgagggt tttgcgagga
      121 tgggtgtgac tccgccgccg gcgcggaccc ggagcgatgt ggcccggccg ccggagcggg
      181 acaaaatccc gacggcccgc ccgtgaaggg tccgcttcgg ggggcgcctc atcctgcgta
      241 aaggtcgtag gtgtcggcgg cgaggatggt ccgggtgacg aacgtgcccg ggcgaagcct
      301 cccgccgcgc acgtaggtga cgccgtcgat gtccggggcc tggcggtggc agcggccgac
      361 gaacgggtac tccggattcg cgctcggtcc tgcgatcagg atctcctgcc gggttccgat
      421 cagcgtccgg ttgatttcct ccgaaatccc ggcctgccgg atcatcagcg tcccgcggcg
      481 tcgccgcttg gtcgcctcgg aaatctccgg cggccggttc tccgcgggcg tgccctcctc
      541 ccgggaatag gtaaaaatcc ccacatggtc gaggcgggcc tccgccaaaa atgcatgcag
      601 ccgattgaac cgcgacggtg tttcccccgg aaaccccacg atcagcgatg tccgcagggc
      661 cacgcccgga atcatggcgc gggcccgggc gatgcgctcg cggatcaggg cgctgttacc
      721 ccgccggtgc atcgcggcga gaagctcgtc gtcgatgtgc tggatgggca tgtcgatgta
      781 tttgcagagc ttggactcgc gcgcgatgac ctcgaacagg ccgtcggtca ggcgctccgg
      841 ataggtgtag agcaggcgga gccatctgag tttcttcatg gaggccaggt cgtgcagcag
      901 cccgggcagg gcgggccgac cggggagatc ctgcccgtag gcggtcgtgt cctgggccgt
      961 gacgatcagc tcccgcaccc cctgtccgac gagggtctcc gcctcacgca ggatgtcccc
     1021 tggctggcgg ctccggtagg ggccccggat tgcggggatc acgcagtagg cgcagcggtt
     1081 gttgcagcct tcggcgatct tcaggtaggc cgtggccgcg ggtgtggaga tgagtcgggg
     1141 aaggccggcg tccatcagga aggttggctt gccggtgcgg ttccggcggc ccgtctgtcc
     1201 cgcgagcagg cgccggagat gagccgcgac ggccgggacc tcgttgacgc cgaggaaaag
     1261 atcgacctcg gggagcgccc gcgcgagcgc cgcgccgtag cgctggggaa ggcatccggc
     1321 gacgatcaga taccgacagt tcccgcccgc cggggtcttg agggcggcca tgcgcaggat
     1381 cgtctcgatc gcctcttccc gggccggcag gatgaaggcg caggtgttga cgagaatgat
     1441 ctgggcttct cgggcctccg ccgtcaggcg aaagccttcc ccggccagca ggccggccat
     1501 cacctcggag tcgatcaggt tcttcggaca gccgaggctg atcatgtgga cggtttcgga
     1561 catggtcagc ggggcagctt gcgggccagg actttccggg gcttgacgcc gtccgagggc
     1621 cccaccacgc cttcggactc catccgttcg atgattcgcg cggcccggtt atagccgacc
     1681 ttgaggtatc gctggaccag ggagatggag gcttgaccga ggtcggtgac cagctccacc
     1741 gcctcgtcgt atttctcgtc gagggcttcc ccctccttgt ccccctcggg cgcgtccggc
     1801 cggtattggg tgatggattc gtcataggcg ggctggccct gctgcttcac atagaagacg
     1861 atccgctcga tttccgcatc ggagacgtag gccccatgga tgcggttgag cttggaggtt
     1921 cccggcggga tgaacagcat gtctccggcg cccaggaggt tctccgcccc ctgcacgtcg
     1981 agaatggtcc gggagtccac cttggaggag acctgaaacg agatgcgggt cgggaagttc
     2041 gccttgataa cgccggtgat gacgtcgacg gagggtcgct gtgtcgccag aatcaggtgg
     2101 atgcccgcgg cccgggccat ctgcgccagg cgggccaggg attcctcgac gttgcgctgg
     2161 gcgacgagca tcaggtcggc cagctcgtcg atgacgatga cgatgtaagg cagcggcgcg
     2221 ggcggcgggg gcgcttccgc ctccggcgac ggatcgccgt ccgacgggag cgaggccgcg
     2281 cgggcgggcg atcctttctt ctccgcggcg gctctcttga cgagttcgtt gaaggcctcg
     2341 attcccttga cgccgcgttc cccgatgatc tggtagcgcc gctccatctc ctccaccgcc
     2401 cagcgaagca cctgggaggc cttcttgggg tccacgacca ccgggtggag gaggtgggga
     2461 atcccttcat aagcggacag ctcgaggcgc ttcgggtcga tcatgaggaa tttcacctcg
     2521 tccggcgggg ccttgaacag aatgctgcag atcatggcgt tcagcgagac gctcttcccg
     2581 gagcccgtcg tgcccgcgat gagcaggtgg ggcatgcgga tcagatccgt aatgacgggc
     2641 tggcccacga tatccacgcc cagggcgatg ggcaggcgac agggggaggt ccggaaatct
     2701 tcatgatcga gaacgtccct caggtggacc tcgtcccggt ccggattggg aatctcgatg
     2761 ccgatcgccg cctttccggg gatcggggcg atgatacgga tgctcggcgc cttgagggcg
     2821 agggccaggt cgtccgtcag attggtgatc cggtgaatct tgaccccggg tgccggctcc
     2881 agttcgtaca tggtgatgac cggtcccgga cggacctcca ggacccgcgc ctcgacgccg
     2941 aagtcggcca gcttgttttc caggacccgg gaattggcga ccagcgtttc cttcttctgg
     3001 cggagttccc gatgctggac ggggtcgagc agggtcaggg gcgggagttg gaatgcctgg
     3061 ccgggggagg gcatcacgag ggggagtggg atctgctccg gcttggccgt gcgacggggc
     3121 tccgcggagc cggcggcgat gacgggcgcg gctttcttct tcgttttctt ttccttgtcc
     3181 ggcttcgaac gccactgccg gagcgcctca attttccgtg ggatcgccac cgaaagcagt
     3241 cggacgcctc cgacgatgac ctggaaaagc cgcctcgcca gaacgaccag ggagaagtcg
     3301 acgaggacca tgagggaaac gatcaggagg gccgtgagga tgagataggc cccggccagg
     3361 ttgaaatagc ccaggaggag acgggcgagc agttggccgg taagcccccc cgtggggaga
     3421 tcgaccccgt ggagcaaaac acgcgggatc gtcacggcga acaggccggt cgtggcgcag
     3481 atcagcccga cgaaacccgc aatgccgacc cccgtgagac ggtacgccct ctccagcagg
     3541 gatttcaggc cggcgatcac caagacgacg gggaaccaga agacgccgaa gccgaacagg
     3601 cgggccaggg tgtcggcggt gtgcgagccg acggtcccgg ccagattgtg aatcggggcg
     3661 tctccagccg tgaaatgggt gaacgaggga tccagcggat gataggaaaa caggctcaga
     3721 aataggaaca gggcgcctgc caggcagacg acaccccgaa tttctcccat cctgttgcct
     3781 tgaatttctt ccgtcatcgg cactccgggg attgcaggcg gatcgcccgc tgttcgtcga
     3841 agttgcggac gatgacctca cgcacctttt ggatcagcgc ctcgcgggtt tcgatcgtgt
     3901 agcccgtcac gtcgatgggc ttgtcgatca ccattgtgat cgttcccggg ttgacccgca
     3961 gggaccgctt cggcatgatt tccgaggctc cgatgatgga aatcggtacg atgggaaccc
     4021 ccgatttgat ggccagatgg aacatgccct gcttgaaggc ctggatcttg ccgtctcggc
     4081 tgcgggtccc ctcgggaaag gtcatgaccg acttcccctc cttgattttt tccgccgcct
     4141 cgtcgatgct ccgcatggcg cgttcgtgat gctgacggtc gatttcgata tagcccgcgt
     4201 ttcgcatggc cttgccgaac agggggatac ggaaaagctc cttcttggcg atccagcgga
     4261 actgcccggg gatgtgggcg agaacgatca ggatgtcgaa atcgctggca tgattggcca
     4321 tgaagatctg cggactgcgg gtgaggacgt tttccccacc gatcacatcg actttggtgg
     4381 cggagatgag aagcaggatc ctggcccaga gattggcgat cttgtggatc ttgttttcgc
     4441 ccggcgagat gtaagggaaa acgatgcaga aaagggagac gaaaccggtg atggcaacac
     4501 cgaggataac caaaagggcg gaacggatca tggtcacctt ccttggacgc gtgggtccac
     4561 ggcggcgctt aaccgcatca tgcgcataat ttcattgaga ttctagcaaa gaaatcatgc
     4621 atatacttct acttgataat tcccatggtg tcaacaggga attggccagg gaaggtgata
     4681 aaagaattga caaaaaacgg acatggtaat agcattcacc cccttgacgc gcctccccgg
     4741 aaaccggcga atcgggatgg acgcacgtcg gggaggaggt tcggccggcc gttatggcgc
     4801 agatacttac gaggaaacgg gcgctggaga tcatggccgg ctttcccctg gcgcgggttc
     4861 tggtcatcgg ggatatcatg atcgaccact tcatctgggg gaaggttgtc cgcatctcgc
     4921 cggaagcgcc cgtccccgtg gtggacgtta aggcggacga cctgatgctg ggcggatgcg
     4981 ccaatgtgct caacaacatc ttctccatgg ggggaaaggc ctgcgtcacc ggggtgatcg
     5041 gcgcggacgt catgggggac aaggtccggg aagagttccg gaagcggcac atctccacgc
     5101 aggggctggt cgcggagccc cggcggccca catccctgaa aacgcgcatt gtggcccatc
     5161 aacagcaggt ggtgcgcttc gaccgggaga gccggacgcc gatccgcgag gagagcgtcc
     5221 gtcacatcct gagctatctg cggaacctgc gtgacagctt gggcgcggtt gtaatttccg
     5281 actatggcaa gggggttgtg acgccggccc tgctggacgg ggtcaggacg gagatttccg
     5341 gccgccccat cgtggtctgc gtcgatccga agcagcggga tttcaacccc tatcggggat
     5401 tcgatgtgct gacccccaat caccatgaag cggccgaggc ggtgggggtg gagatgggga
     5461 acggggccga gctcgttgcg gtcggaaaac agctcatcga gcgatacgat ctgaaggccc
     5521 tcttgatcac ccggggggaa gcgggcatga gcctcttcga gaggcgcggc cgcatcgttc
     5581 atacgcacct gccgaccgag gcccgggagg tcttcgacgt caccggggcc ggtgacacgg
     5641 tcatcggggt gttcgccctg tgcgtggcct ccggcgcgac ctttcgagag gcggcggttc
     5701 tcgccaatca cgccgccggg atcgtggtcg gcaaggtggg aacggcgacg gcctcccgcg
     5761 aggaactcaa gcgcgtccta tgagcaggct cgcaacggcc ccggtcgtga aactcatcat
     5821 gagcgttctt gctccagcgc cggatctcat cggacaggcg gtcggggtcg tctcggacct
     5881 ctacggggag gcggatttcg tgagcgccct gtttccgttc gactatacgg attattacgc
     5941 cggggagatg ggcgctcccc tgtacaggcg gtttgtcgct ttcgagcggc tgatgccgcc
     6001 ggaaaccctt ccggacgtca agcgtctgac ctgtgaggcg gaagagcaat ggacagaggg
     6061 gggacggcgg cgggtcaaca tcgatccggg ctatcttgcc gaggcccatc tgatcctagc
     6121 cacgggaaag ggctacgccc accggcccta tctgcgtgac ggagtctatg cggatctgac
     6181 cctggtgtat cagcaccagg cgttccggcc cctggcctgg acgtatccgg attatggcag
     6241 cccggacgtg agggccctct tcgaaaagat ccgggcaaaa tacctgctgc agcggaaaga
     6301 ggcgcgccat tgaccgggcg tctggggccg tccgttccga atttacgtgt attctgaaga
     6361 aatgactcgg gcgcgatccg ccgggaaggg attcgagacg atatgaagag catgacgggg
     6421 tacggccgtg ccgagggaat gtgcgggggc aaaaaactcg tggtggagat ccggtccctc
     6481 aaccaccgat acatcgaggt gtcgctccgg ctgccgcccc cctttttccc ctgggaattg
     6541 gaaatcaaga aacgggtgac cgaacgcttt tcccgcggca agatcgaggt cgccctgcgc
     6601 atggatgccc aggaggggtt ggaggccgcg ggccggctga ccctgaattg gccgcttttg
     6661 cgccaatacc acgccctgct ggtggagctc aagcaggcct tcaacatcga ggatccgatc
     6721 accctgtcga tgctggcggg gctccgggag gtcttcatca cgtccgagaa cggcgctcct
     6781 gcggagaacc tgtggccgga tgtggagccg atcctcgcgg aggccatgtc catgctgtcg
     6841 tccatgcggc agcgggaggg ggagatgctc cggcaggaca tggtgatgcg tctccagttg
     6901 attcaaggcg agatggacgc gattcggaaa cggtcgcccg aggtcgttct cgaatgccgc
     6961 gggcgcctgg cggagcgggt gcgggaactg gcgaacaacc tggagatcga tgagggccgg
     7021 ctgagcgtgg aagtggcgat catggccgac aagatggata ttaccgaaga gttggtgcgt
     7081 tttgccagtc acctgaacca gtttcacgag ctggtcctca gtcccgacgc cgtgggccga
     7141 aaaatcgatt tcctcattca ggaactgaac cgggagatca acacgatcgg gtccaagagc
     7201 gccgatacgg acattgcccg gcgggtcatc gatatcaaga gcgagctgtc gaaactgagg
     7261 gaacaggtgc agaacattga gtgatccggc cggccggaca aatggaatgc tgatcgtcgt
     7321 ctccgcgccc tcggggaccg gcaagacgtc gctgtgccgg gaggttctgg cgatgttccc
     7381 ggagctcagg ttttccgtct cctggacgac gcgtcctccc cggccggggg agcgggacgg
     7441 ccaggattac tgctttacgt ccgaagcgga gttccggcgc cggatcgagc agggggagtt
     7501 tgtcgagtgg gtggagaact acggacacct ctacggcacc tcgatccgga ccgttcaatc
     7561 ttttctggat cagggtcacg acctcctgct tgacgtggat ccgcgcggcg ccagggcgat
     7621 caaggagcgg tttccgtcgg gcgtgttcat ttttctgctc cccccgtcgc tagcagcgct
     7681 ggaggtccgg ctgtctcgtc gcggggaatc cgcggacgtg atggccgcgc gtctcgcgaa
     7741 agcaaaggat gaaattcgcg agatgatctg gtatgattac ctcatcgtca acgaacagct
     7801 cgatgaggcg gtggattgtc tgcgggcggt ctatatggcg gaaacctgtc gccgggagcg
     7861 gttctacgac cggatgaagg gcgatttaca atccctgacg ggatgagagg gaggcgtaaa
     7921 gttatggcga gaattacggt ggaggattcc ctgcgaactg cgaagaaccg gtttgccctg
     7981 gtaatcctga cggccaagcg ggcgcggcag ttgctgaaag ggtcgcagcc cctggcggag
     8041 agaaagaaca accgggaaat cgtgacggcg cttcgcgaga ttgccgacgc ccaggtcgtt
     8101 ttttcccatc ccgaatacct gcggggcatc aaggaggagt accgtccggt gctcgatcaa
     8161 acggaattcg ttggtgatga agaatatacg gagtgacgct ccccgcgagc gcctgatctt
     8221 tgccctcgat gtgggcgagg ggctgacgga ggtcttgtcc tgggtgaagc gtctccggga
     8281 tcacgtcggc tgcttcaagg tggggaagga ggccttcacc cacctggggc cgggcttggt
     8341 ggaggcgatc caccaggggg gcggccgtgt ctttttggat ctcaagttcc acgatattcc
     8401 caacacggtg gcccgcgccg ccgaggcggc ggtccgcatg ggagtgaaca tgtttaacgt
     8461 gcacgccttg ggcggctccc ggatgatggt ggagaccgcg accgccgtcc ggaacatggc
     8521 cgcgcgcctg gacgtgcccg cccccgttat cctggccgta acggtcctga ccagtctgga
     8581 cgaccgggat ctaagcgatc tcgggttccg catgtccacg gaagcgctgg tggtccatct
     8641 ggctgtacag gcccgggccg cgggactcga cggtgtggtc gcttctgcac gggatgtccc
     8701 ggccatccgt gccgcctgcg gcgaggcgtg tctcatcgtc acgccgggca tccgtcggga
     8761 cggggtcgat gccggtgacg accagaagcg gatcctgtcg ccggaaggtg ctgttgccgc
     8821 cggggccgac tatctcgtgg tgggccgccc gatccgcatg gccgtggatc cggcggcgga
     8881 agcggacgac atcgtccggg cgatcacggc tggcctcgcc acgcgcaccg gggcaccgcc
     8941 gcgcgtttga gaggcgggaa cggcatgagg gcgtcgaacg gcggatgcca ggaaatcctc
     9001 tacgggataa accccgtgcg cgaggccctg cgtcacgggg gcgtggacat ccgggaaatc
     9061 tgggtgacgg atgggcgggg aggggcgccg ctcacggaga tcctggaaca ggcgcggcaa
     9121 agggggattc ccgtggctta ccggcgccgg gaagaactgg accggcggtc cggtgtgcgc
     9181 agccaccagg gcctggtcgc cgtcggtcgc gcctttgcct atgccgatct cgaagaggtg
     9241 gtggcaaatc gtcacgaggc gtttcggcac ggttgtcttt tgattctgga tggggtcctc
     9301 gatccccaga acctgggatc cctgatccgc acggcccact gtttcggtgt caacggcgtg
     9361 atcattccgg agaaccgcgc cgcggccgtc agccccgccg cggttaaggc ctcggcaggc
     9421 gccgccggac acatccccgt ggcgagggtg gtcaacctcg ttcagaccat ccagttgttg
     9481 aaagaccgag gattctggat ttatggcgcc gatacccggg ggggaagaca accggaggaa
     9541 ctcgtgatcg agggcgatat cgccctggtc atgggcagcg agggcaaggg gatccggccg
     9601 ctggtgagac gccagctgga ttttcccctg tccattccct tggtggggtg cctcgattcg
     9661 ttgaatgttt ccgtggcggc gggaatcatc ctgcatgcga ttatgaaaca gcggacccgt
     9721 cagtagacat ccttgatcat caccgagacg gagggaacca tgccggcatt cctgtgggaa
     9781 gcgacatccc ggaaggggga gcagaaaaag ggagaactgg acgcggcgga tgaggcgacc
     9841 gtacgcgctc agttgcggcg tcagggtttc aagaacatca gcgtcaaggc caagccgaaa
     9901 gacctgatgg aatacctccc ctttcttcag cagtcggtga aggagaagga cgtggtggtg
     9961 ttttcccgga tcttctcgac catgatcaat gcgggcctgc ccctgatcca gtgcctcgaa
    10021 ctcctggcga gccaggagca gaataagaca tttgccggga tcatccgggc catcaaggag
    10081 gacatcgaag gcgggtcgac actgacggac gccctcaaaa aataccccaa gatcttcgac
    10141 gaactgttcg tcaacctcgt ggccgcaggg gaggccggcg gcattctcga cgtcattctg
    10201 tcacgccttt cggcctacat ggaaaaggcc atgaaactga agagcaaggt caagggggcc
    10261 atgacctacc cggccagcgt cctggtgatc tccatcggtg tcgtggccgt ccttctgctc
    10321 aaggtcatcc cggtctttca gaagatgttc gaagggatgg gcggcacgct gccggcgccg
    10381 acccagtttc tcgtcaacgc aagccagttc gcccagaatt acttttacgt gtttatcatc
    10441 gtcattgtcg ctgtggtctt cgcctacaaa cgctttgtcg caacggaaaa ggggcggtat
    10501 gtcgtcgact ccctggctct caaggcgccg gtgttcgggc aactcatcag gaaggtagcc
    10561 gtatccaaat tcacacggac catgtccacc atgatgtcga gcggcgtgcc gattctggag
    10621 gcgctcaaca ttgtcagcaa gacggcgggc aacgtcatca tcgagaatgc cctgatacag
    10681 acgcgcaaga gcatcagcga aggacggaca atcgccgagc cgctcatgga gacggatatc
    10741 ttccccccca tggtggtgca gatgatcgcc gtcggggagg caaccggggc gctcgatacg
    10801 atgctggcga agattgcgga cttctacgac gacgaggttg acgcggccgt ggatgcgctg
    10861 acctcgctcc tggagccgtt catgatggtg tttctgggcg gcgtcgtggg cgggatgatc
    10921 atcgccatgt acctgccgat cttccagatg gcctcggtgg tgggcggata aaaaaatggt
    10981 cgggatggcg cgatttgaac gcgcgactcc tgcgtcccga ac
//
LOCUS       DAOOLJ010000063         9912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_7854_length_9912_cov_17.212438, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000063 DAOOLJ010000000
VERSION     DAOOLJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7854_length_9912_cov_17.212438"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1772)
                     /locus_tag="PLW16_08650"
     CDS             complement(330..1772)
                     /locus_tag="PLW16_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPB71016.1"
                     /translation="MATATLLRKVLKLRTVISTSAGMAMATSCYLAGMQVATMLAGEL
                     AWISILVAGSLCLLSALCFSELTAQYPTAAGIKLFIQNAFNERVAIAIGMFYVLMGIT
                     MVGAESYLLSSVLMDSLPLVSPAFDRVLWVIAFIAFVGFINVRGVKLTGLVQDILTYA
                     MLLFLVGVSLYTFTRVGVDIPAALAHPKLTWGNVLNAAGVGVFLYVGFEWVTPLAEET
                     TDYRMIGRGMLWAIGLLSITYSLFTVAMVTGLSHLAPEFIRRGTDIPHIEFGRALFGS
                     GGVLFFVAMSILASVTSFNSGLLNTSRFAYAMARDNVLPKIFSRIHPDYATPWFAILG
                     LMVFALVVCFLILFSGRYMFIIILAAALECFIYVVMALCVIRLRRKFPDRERSFKVPG
                     GYLVPALTVVIFLGLLLGIFCDVTRDHTGKILFANGWVALVMAGFFLGTALYTLYVVP
                     IFKKKAAELAGKRVKRRPGRTAGAGTEAGE"
     gene            complement(1783..2142)
                     /locus_tag="PLW16_08655"
     CDS             complement(1783..2142)
                     /locus_tag="PLW16_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPB71017.1"
                     /translation="MATGAEILEALADYQVQCNENKRLRRMQRDWSRLLHFVADDTGD
                     IFTMDVIQGEIVSYAAGAAGTPDIIVTTASENLCDMFWGDLNPSQKYLQGEIRVKATQ
                     EDIVRLDAITMIIWPDV"
     gene            complement(2210..3478)
                     /locus_tag="PLW16_08660"
     CDS             complement(2210..3478)
                     /locus_tag="PLW16_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB71018.1"
                     /translation="MSDDLNTLKQRALADFAEFVNPMKVRTMKAAGIDLIEERRDGAC
                     TWDLTGKRFIDCQTGSGIMNVGRHNREIVEALKKALDTYDIGVFLVCSRQKADLARKL
                     AEITPGDLKCTVYGAGGGEAVDAAIKIARGYTMKTEIVYADKAYHGHTGFALSAIGRA
                     AYKEPFEPLMPGFKQVPFGDAQAVREAMSADTAAVILEPIQGEGGINVPPDDYLPEVR
                     RLCDEHEALLILDEIQTGFARTGRMFACEHWGVVPDILTLAKSLGGGLYPISATVFKE
                     EINDFFIPHPFVHLSTFGGSDLGCIVALAVIDYIEKNGLAEHARLMGERFRAGFDRLT
                     AAYPDLLLEVRQKGLMMGLQYTNTSIGPRMTKKLADRGVLAIYTGNDPSICRLMPPLV
                     ITPAEVDEVLAALEGAMRELSGEKGLGKDD"
     gene            3765..4673
                     /locus_tag="PLW16_08665"
     CDS             3765..4673
                     /locus_tag="PLW16_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB71019.1"
                     /translation="MEYQSVDDVQERLTAAGYIPSREIATVVFLAEATQKPILVEGPA
                     GVGKTELAKAVARSLNRELIRLQCYEGLDEAKALYEWEYAKQLLYTQMVKGRINDIIA
                     DADTLTVAVDRIAAQEDAFFSDRFIQPRPLLQAISSEEPVVLLLDEVDKSDPEFEAFL
                     LELLSDFQVSIPELGTRKAVRIPFVLLTSNNYRDMSDALKRRCVHLYIDYPERDLEIR
                     IVRLKVPGVEEALVRSLVEAIRRIRALDLKKNPSISESIDWAQSLLALQARSLSPGVL
                     SDTLNVVCKYQGDIEKVRKNLDRVLA"
     gene            4684..6069
                     /locus_tag="PLW16_08670"
     CDS             4684..6069
                     /locus_tag="PLW16_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB71020.1"
                     /translation="MVDLVLKFVSSCRASGMRISTSEVLDCVRHLERIDCTREDEFRA
                     ALRANFAKSVRELAHFDRLYRLFFHEFRLDAADVEAAGRFSNQIGDLARRLAPEAAPE
                     AEYRAVVDFLAGDPQSLLRALHRIRTESDASPQTVKFNLAPLEGRLKLLMQILRVGDV
                     AQALIKDEKLDLDAEDRRALAAHVGERLDRARALLMFEPLPNADGIRKNGDAGERLKQ
                     LGEKPFSSFTPRETEEMREAIGGLVRKLKNITSRRFAVRNRGALDVKKTLRAAARYNG
                     VPLDLKYRRKALRKGRIVTLCDVSGSVWSAARFMLNLLYSLQDCFDRVHSFVFVDRLT
                     EVTEIFDTHEVNEAIGRVLGKAGVGFNATTDYGEMFRHFKRGYMEILTKKTTLIVIGD
                     ARSNYLSPEDAILGEMRDRCRRVIWLNPEPEAIWGTADSEIYTYAPWCHEVRPCRNLN
                     QLIRFIEELVL"
     gene            6069..7199
                     /locus_tag="PLW16_08675"
     CDS             6069..7199
                     /locus_tag="PLW16_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB71021.1"
                     /translation="MTGDLHAETRRVRERFTRFAAERIAGRGDLDNGEAFPGDLWRRM
                     GEAGLFRLGIPKRYGGSGGGWPALVAAGEALVASGGNLGLALSWLVQQAVARFLVHGF
                     GNAGQRGRLLPELALGRLTVAFAVSEPGHGAHPKHLTTRAERAGDGFRLTGEKAYLTN
                     GPIADLYVVVAVTSEAEGRKRLTAFLVPRRTPGLSVAAPTPLPFLRPSPHGGIELVDC
                     RLPGTAVLGEAGTAYETMVIPFGGVEDLLMTGAILGALSRLEARAVEALRAGEPDRTL
                     RSELGTVHALREMLTGVALAAAADLDRGVAPSGIPQTVAGFPLAVALLERHAAILSRT
                     GAGEDPVPARLQADLTALFSLSRRRLIARQERLGAALISNPA"
     gene            complement(7434..8723)
                     /locus_tag="PLW16_08680"
     CDS             complement(7434..8723)
                     /locus_tag="PLW16_08680"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPB71022.1"
                     /translation="MEKAELIYGGKTIGLPVVTGTEGEKAIDISRLRQESGLITLDVG
                     YQNTGSCMSEITFIDGDRGILRYRGIPIEELAERASFSEVAYLLIYGDLPNRAEQAVF
                     SQYLTQNSMIHEEMLRFFDGFPPTAHPMAILATMVNAMSVYYTDYYTEDFQAQTFDLM
                     AASLISRIRTIAAYSYKHSIGQPHIYPKPDLKYTANFLYMMFDSPVDPYVPDPDIEKV
                     LSTLLILHADHEQNCSTSTVRLVGSSMANLYASICSGICALWGPLHGGANQKVIDMLE
                     NIDRNGISIEECLASAKDKNSRVRLFGFGHRVYKSHDPRARILKQCAKTIFRNSDRRD
                     SLVNIAIELEEAALKDEFFIERKLYPNIDFYSGILFRMIGIPTNMFTVMFAIGRLPGM
                     IAQWKEMHDATPLKIGRPRQIYTGSTLRHYIPLEERP"
     gene            complement(8876..9802)
                     /locus_tag="PLW16_08685"
     CDS             complement(8876..9802)
                     /locus_tag="PLW16_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB71023.1"
                     /translation="MGKSLGINNIPSKVCTYACRYCQVGRTTSMTLDRRAFYRPEEIL
                     RDVQDRIAMTRAAAEPIDYLAFVPDGEPTLDIHLVDELMMMKSLGIPVGVITNGSLLW
                     REDVREALAGADWVSLKIDAVEERIWRRIDRPHKMLNLSSILAGMGQFAETFAGKLVT
                     ETMLVKGINESETCLAGIAGFIKGLKPSAAYLSIPTRPPTLERVCAPDEETVNRAYRI
                     FADRLERVEYLIGYEGDAFAFTGDIQKDLLSITAVHPMRREAVDALLAKAGASWAVVE
                     NLQAQGELAEVRHDGHLFYLRKFTKTRPSDAT"
BASE COUNT         1835 a         3125 c         3120 g         1832 t
ORIGIN      
        1 tttttacgtt accacaaccc cttcaggtgg gtcagttttc ggttaccaca atcaagattt
       61 tctataaccg gcaaagaaag caggccagac tcggtttttt atctcccgtc gcttttgaac
      121 gatggttcta cgaaaaacga ctggctgcgt gaaaggtttg gtgtctacta ttgacgaccg
      181 acctcagtcc ctgtttttta caccaaggga tcgggatggg cccatcgtgt ttttgccgcg
      241 atccctatgc tttgtgatcg ctggttccgg cggggcttcc ggcgcgggcg gaggggaacg
      301 ggataaggga ggagcggata aaagaaatct tactctccgg cctcagtgcc ggcgccagcc
      361 gtccggcccg gacggcgctt gacgcgcttg cccgccagct ccgcggcctt ctttttgaag
      421 atcggaacca cgtacagcgt gtagagggcc gtgccgagga agaatcccgc catgacgagg
      481 gccacccagc cgttggcgaa gaggatcttg cccgtgtggt cgcgggtgac gtcgcagaag
      541 atgccgagca agagccccag gaaaatgacg acggtcagcg cggggacgag gtagccgccc
      601 ggcaccttga agctccgctc ccggtcgggg aacttccgcc gcaggcggat gacgcacagg
      661 gccatgacca cgtagatgaa gcactccagg gcagcggcca ggataatgat gaacatgtac
      721 cggccgctga agagaatgag gaaacacacg acgagggcga agaccatcag gcccagaatg
      781 gcgaaccagg gggtggcgta gtccgggtgg atccggctga agatcttcgg caggacgttg
      841 tcgcgggcca tcgcgtaggc aaagcgggag gtgttcagga gccctgagtt gaaggacgtc
      901 accgaggcca gaatgctcat cgccacgaaa aagagcaccc caccgctgcc gaacagggcc
      961 cggccgaact cgatgtgggg gatgtcggtt ccccgccgga taaactcggg ggccagatgg
     1021 ctcagccccg tgaccatggc aacggtgaag agcgaatagg tgatggacaa aaggccgatg
     1081 gcccagagca tcccgcgccc gatcatccga tagtccgtcg tttcctcggc gagcggcgtc
     1141 acccactcga agccgacata gaggaagacc cccaccccgg cggcgttgag cacgtttccc
     1201 caggtcaatt tcggatgcgc gagggcggcc gggatatcca cgccgacccg cgtgaaggta
     1261 tagagggaca ccccgacgag aaacaaaagc atcgcgtagg tcaggatgtc ctggaccagg
     1321 ccggtgagct tcaccccccg gacgttgatg aacccgacga aggcgatgaa ggcgatcacc
     1381 cacaggaccc ggtcgaaggc gggactgacc agcggcaggg aatccatcag gacgctggac
     1441 agaaggtagc tctccgcgcc gaccatcgtg atccccatca ggacgtagaa catcccgatg
     1501 gcgatcgcaa cccgctcgtt gaaggcgttc tggatgaaga gcttgatccc cgccgcggtc
     1561 gggtactggg cggtcagttc cgagaagcac agggccgaca ggaggcacag cgaccccgcg
     1621 acgaggatgg agatccaggc gagttcgccc gccagcatcg ttgccacctg cattcccgcc
     1681 agatagcagg acgtcgccat ggccatgccg gcggaggtgg aaatgaccgt ccgcagtttg
     1741 agcaccttcc gcagcagcgt cgccgtcgcc atgcgctcct ccttaaacgt cgggccagat
     1801 gatcatggtg atggcgtcga ggcggacgat gtcctcctgc gtcgccttga cgcggatttc
     1861 cccctggagg tacttctgcg agggattgag atcgccccag aacatgtcgc agaggttttc
     1921 actcgccgtc gtgacgatga tgtccggggt gcccgcggcc cccgccgcgt aagagacgat
     1981 ctccccttgg atcacgtcca tcgtgaagat gtccccggtg tcgtccgcga cgaagtggag
     2041 cagccgggac cagtcccgct gcatccgccg cagacgtttg ttttcgttgc actgtacctg
     2101 gtagtcggcg agcgcctcca gaatctccgc accggtcgcc ataggacgct tcccccctgc
     2161 tgatggttgc agacactttc ccccgggatc acgcgccccg gggccgccgt cagtcgtcct
     2221 tcccgaggcc cttctctccg gaaagctccc gcatggcgcc ctcgagcgcg gcgaggacct
     2281 cgtccacctc cgccggggtg ataaccagcg gcggcatcag tcggcaaatg ctcgggtcgt
     2341 tgcccgtgta gatcgccaga acgccgcggt cggcgagctt cttcgtcatc cgggggccga
     2401 tggaggtgtt cgtgtactgc aggcccatca tcagcccctt ctgccggacc tccaggagca
     2461 gatcggggta ggccgccgtc aggcgatcga aaccggcgcg gaaccgctcg cccatgagtc
     2521 gcgcgtgttc ggccaggccg ttcttctcga tgtagtcgat cacggcgagc gccacgatgc
     2581 accccaggtc tgagccgccg aaggtggaca ggtgaacgaa agggtggggg atgaagaagt
     2641 cattgatctc ctccttaaag acggtggcgg agatggggta gaggccgccc cccagggatt
     2701 tggccagggt caggatgtcc gggacgaccc cccagtgctc gcacgcgaac atccggcccg
     2761 tccgggcgaa gccggtttgg atttcgtcga ggatgagcag cgcctcgtgt tcgtcgcaga
     2821 ggcgacggac ctccggcagg taatcgtccg gcgggacgtt gatccccccc tctccctgga
     2881 tcggttcgag gatcacggcg gcggtgtccg cgctcatcgc ctcccgcacg gcttgggcat
     2941 cgccgaaggg cacttgcttg aagccgggca tcagcggctc gaacggctcc ttgtaggccg
     3001 cccggccgat ggcggagagg gcgaagcccg tatggccgtg ataggcctta tcggcgtaga
     3061 cgatctccgt cttcatcgtg tagccgcggg cgatcttgat cgcggcgtcc accgcctccc
     3121 cgccacccgc gccgtagacc gtgcacttca ggtcgccggg ggtgatctcg gcgagtttcc
     3181 gggcgagatc ggccttctgg cgggagcaaa ccaggaacac gccgatatcg taggtgtcca
     3241 gcgccttctt gagcgcctcc acgatctcgc ggttgtggcg cccgacgttc atgatgcccg
     3301 agcccgtctg gcagtcgatg aagcgcttgc cggtgaggtc ccaggtgcag gcgccgtccc
     3361 gccgctcctc gatcaggtcg atgccggcgg ccttcatggt ccgcaccttc atgggattga
     3421 cgaactcggc gaaatcggcc agtgcccgct gcttgagggt gttcaaatcg tccgacacgg
     3481 tgtcacctcc ttttgaatga ggttcttgct tctccgcgat ccgggcatgc cgccgcctgt
     3541 tcaacaggag acattctggt gctcagcaat aagacgaggg caatggatgt aaacaggcgg
     3601 tgcatgcata cctccatcga ctgaagcggg gcgaattttt gtgacgtgat gaccgaaggt
     3661 aagaggaacc gcccccgtat gtcaaggtaa ttttgacacc ccgccgggaa ttccttgacc
     3721 tgtggggttg attggtataa aagcccgaaa aaaaggacgt tttcatggaa tatcagagtg
     3781 tggacgatgt ccaggaacgc ctgacggcgg ccggctacat cccctcccgg gagatcgcga
     3841 cggtggtctt cctcgcggag gcgacgcaga agccgatcct cgtggaaggg cccgccgggg
     3901 tcggcaagac ggagctcgcc aaggccgtgg cccgcagcct gaaccgggag ctgatccgcc
     3961 tccagtgcta cgaggggctg gacgaggcga aggccctgta cgagtgggaa tacgccaagc
     4021 agctcctcta cacccagatg gtcaagggcc gcatcaacga catcatcgcg gacgcggaca
     4081 cgctcaccgt ggcggtggac cgcatcgccg cccaggagga cgccttcttc tccgaccgct
     4141 tcatccagcc ccgcccgctc ctccaggcga tcagctcgga ggagcccgtc gtcctcctcc
     4201 tggacgaggt ggacaaatcc gatcccgaat tcgaggcgtt cctgcttgaa ctcctgagcg
     4261 atttccaggt ttcgatccct gaactcggga cgcgcaaggc cgtcaggatc ccctttgtcc
     4321 tgctcacctc caacaactac cgcgacatga gcgacgcgct caagcgccgg tgcgtccacc
     4381 tgtatatcga ctacccggag cgggatctgg agatccggat cgtgcgactc aaggtcccgg
     4441 gggtcgagga ggcccttgtg cggagcctcg tcgaggcgat ccgccggatc cgcgccctcg
     4501 atctgaaaaa gaaccccagc atctccgaat ccatcgactg ggcacagtcc ctgctggccc
     4561 tccaggcccg gtcgctgtcg cccggggtcc tatcggacac cctgaacgtc gtctgcaagt
     4621 accagggcga catcgaaaag gtccggaaaa acctcgaccg cgtcctcgcg tgagtcccgc
     4681 gccatggtcg atctcgtcct gaaattcgtc tcctcctgcc gggcgtcggg gatgcggatc
     4741 tccacctccg aagtcttgga ctgcgtccgg catctggagc ggatcgactg cacccgcgag
     4801 gacgagttcc gggcggcgct cagggcgaac ttcgcgaaga gcgtccggga gctggcccac
     4861 ttcgaccgcc tttaccgcct cttctttcac gagttccgcc ttgacgccgc cgatgtggag
     4921 gccgccggac gtttcagcaa tcagatcggt gacctcgccc ggcgtctcgc gccggaggcc
     4981 gccccggagg cggaataccg ggcggtggtg gattttctgg ccggggaccc ccagagtctc
     5041 ctccgggcgc tgcaccgcat ccggacggaa tccgacgcct ccccgcagac ggtgaagttc
     5101 aacctggcgc cccttgaagg ccgcctgaaa ctcctcatgc agatcctgcg ggtgggcgac
     5161 gtggcgcagg cgcttatcaa ggacgaaaag ctggatctgg atgcggagga ccggcgtgcg
     5221 ctcgccgctc acgtcggcga gcgcctggac cgcgcccggg cgctgctgat gttcgagccg
     5281 ctacccaacg cggacggcat ccgcaagaac ggcgacgcgg gggaacgcct caagcagctc
     5341 ggcgaaaagc cgttctcgtc cttcacccca cgggaaacgg aagagatgcg cgaggccatc
     5401 ggcggcctcg tgcgcaagct gaaaaacatc acgagccgcc gcttcgccgt ccggaatcgg
     5461 ggcgccctcg acgtcaagaa gaccctccgg gccgccgccc gctacaacgg cgtgcccctc
     5521 gatctgaaat accggcgcaa ggccctccgg aagggccgga tcgtcaccct ctgcgacgtt
     5581 tcgggctccg tctggtccgc cgcgcgcttc atgctgaatc tcctgtattc cctccaggac
     5641 tgtttcgacc gtgtccacag tttcgtcttc gtggaccggc tgacggaagt cacggagatc
     5701 ttcgacacgc acgaggtcaa cgaggcgatc ggcagggttc tcgggaaagc cggtgtcggc
     5761 ttcaacgcca cgacggacta cggggagatg ttccgccatt tcaagcgcgg ctacatggag
     5821 atcctgacga agaagacgac gctcatcgtc atcggggacg cccgctccaa ctacctgagc
     5881 ccggaggacg cgatcctggg cgagatgcgc gaccggtgcc gccgggtgat ctggctcaac
     5941 cccgaaccgg aggcgatctg gggcacggcg gacagcgaga tctacaccta cgccccctgg
     6001 tgccacgagg tgcgaccgtg ccggaacctg aaccagttga tccgcttcat cgaggagctc
     6061 gtcctgtaat gaccggtgat ctgcatgcag aaacgcgccg agtgcgtgaa cgcttcacgc
     6121 ggttcgccgc ggagcgcatt gccggacgag gggatctgga caacggagag gccttccccg
     6181 gcgacctgtg gaggcgcatg ggcgaggcgg gtctcttccg gctcggcatt ccgaagcgct
     6241 acggcggcag tggcgggggc tggccggccc tcgtggcggc cggcgaggcg ctcgtcgcct
     6301 ccgggggaaa cctggggctg gcgctttcct ggctggttca gcaggccgtc gcccgcttcc
     6361 tcgtccacgg tttcggcaac gcgggacagc gggggcgcct cctgccggag ctagccctcg
     6421 gccgtctgac cgtcgccttt gccgtctcgg agcccggcca cggcgcccac cccaaacacc
     6481 tgaccacgcg ggcggagcgg gcgggcgacg gattcaggct cacgggtgaa aaggcctacc
     6541 tgacgaacgg tcccatcgcc gatctttacg tcgtcgtcgc cgtcacctcc gaagcggagg
     6601 gccgaaagcg cctcacggcc tttctcgtgc cccggcgcac gcccggcctc tccgtggcgg
     6661 cgcccacgcc gctgccgttc ctgaggccgt cgcctcacgg cggcatcgaa ctcgtcgact
     6721 gtcgcttacc ggggacggcg gttctgggcg aggcggggac ggcctacgag acaatggtga
     6781 tcccgttcgg cggggtggag gacctgctca tgacgggggc gatcctgggg gcgctttcaa
     6841 ggctcgaggc gcgggcggtc gaggcgctcc gtgccgggga acccgaccgg accctgcgga
     6901 gcgagctggg caccgttcat gccctgcgcg agatgttgac gggggtggcc ctggccgcgg
     6961 cggcggacct ggaccggggg gtggccccgt ccgggatccc gcaaacggtt gcggggtttc
     7021 ccctggccgt ggcgcttctc gagcgtcacg cggccatcct ctcccgcacg ggcgcgggcg
     7081 aagatcccgt gccggcgcgg ctccaggccg atctcaccgc cctgttctct ctcagccgcc
     7141 gccgtctgat cgcccgccag gagcgcctcg gcgccgcgct catttccaat cccgcctgac
     7201 gaacatcgca accggatgct ggtgcatcaa gggaatcgga tccccggcgc accgcgttcc
     7261 caatagagat gggcgaagaa gaacgtaaat aagcaaaagg cggggcctat tggccccgcc
     7321 ttttgtcttg atcatataac gcttggcatt cgtcgccacc cgcgcggggg cgtggattga
     7381 aacacctggg gcaccgccac gagcacctgc ggatgccgac gccgccgatc ggcctacggg
     7441 cgttcctcca gggggatata gtgcctcagc gtgctgcccg tgtaaatctg gcgggggcgg
     7501 ccgattttca agggcgtggc gtcgtgcatc tccttccact gggcgatcat gcccggcagg
     7561 cggccgatcg cgaacatgac ggtgaacatg ttcgtcggaa tgccgatcat ccggaataaa
     7621 atgccgctgt agaaatcgat gttggggtag agttttcgtt cgatgaagaa ctcgtccttc
     7681 agggcggcct cctcgagctc gatggcgatg ttcaccaggg aatcgcggcg gtcgctgttc
     7741 ctgaagatcg ttttggcgca ctgtttcaga atcctggcgc gggggtcgtg actcttgtac
     7801 acccgatggc cgaagccgaa cagacgaacc cggctgtttt tgtctttggc cgacgcgaga
     7861 cattcctcga tggagatccc gttcctgtcg atgttttcga gcatgtcgat gaccttctgg
     7921 ttcgccccac cgtggagagg cccccagagc gcgcaaatgc ccgagcagat cgacgcatac
     7981 agattggcca tgctgctgcc gaccagccgc acggtcgatg tgctgcaatt ctgttcgtga
     8041 tccgcatgca ggatcaaaag cgtggacagc accttttcga tgtccggatc cggtacgtag
     8101 gggtccaccg gggagtcgaa catcatgtaa agaaaattgg ccgtgtattt caggtcgggt
     8161 ttgggataga tgtgcggctg accgatgctg tgcttgtagg aatatgcggc gatcgtgcgg
     8221 atccgggaaa tcagcgaggc ggccatgaga tcgaaggtct gcgcctggaa atcctccgtg
     8281 tagtagtccg tataataaac ggacatggcg ttgaccatcg tcgccaggat ggccatcggg
     8341 tgtgccgtgg ggggaaatcc gtcgaagaaa cgcagcatct cctcgtgaat catggaattc
     8401 tgcgtcaggt actgcgagaa aaccgcctgt tcggcccgat tgggaagatc cccgtaaatc
     8461 agcaggtacg ccacttccga aaaactggcc cgttccgcca gctcctcgat cggaataccg
     8521 cgataacgca atatgccgcg atccccatcg ataaaggtga tctcggacat gcaggatccc
     8581 gtattctgat accccacgtc cagggtgatc agaccgcttt cctgccgcaa ccgggatatg
     8641 tcgatggcct tttccccttc cgtgccggtc acaaccggaa ggccgatcgt ctttccgccg
     8701 taaatcaatt ccgccttttc caatgcctct tctccaatcc gctgctcagt ctttagctgt
     8761 tatggcgtcc ttagctgcta tagcggcttc tgtcaacggt catttgaagg acgtccggcc
     8821 gcccgatgcc ggcaatccgg tgcagggatt ccgtgcgccg attgttttgc attcgctatg
     8881 tggcgtcaga ggggcgggtt ttagtgaatt ttctcagata aaagagatgg ccgtcatgtc
     8941 tcacctctgc cagttctccc tgcgcctgca gattctccac gacggcccat gaggccccgg
     9001 ccttcgctaa aagcgcgtct accgcctctc gtcgcatcgg atgaacggcg gtaatgctca
     9061 agagatcctt ttggatatcg ccggtaaagg caaacgcatc cccctcatag ccgatgagat
     9121 attccacacg ctccaaccga tcggcgaaga ttcggtacgc ccggttcacg gtctcttcat
     9181 cgggcgcgca aacccgctca agggtcggcg gccgggtcgg aatgctcaaa taagccgcag
     9241 agggcttcaa acccttaatg aatcctgcga tccccgccag gcaagtttcg ctttcgttga
     9301 tgcccttgac gagcatcgtt tcggtgacaa gcttgccggc aaaggtttcg gcgaattgcc
     9361 ccattcccgc aaggattgag gacaggttca gcattttgtg gggacggtcg attcgcctcc
     9421 agatccgctc ctcgacggca tcgattttca gcgaaaccca atctgctccc gcgagcgcct
     9481 ccctcacgtc ttcacgccag agaagagatc cgtttgtgat gacgccgacg gggatcccca
     9541 gagacttcat catcatcagt tcgtcgacga gatggatgtc cagggtaggc tctccatccg
     9601 gaacgaaggc gaggtagtcg atgggctcgg ctgcagccct cgtcatcgca atcctgtcct
     9661 gcacatcccg aaggatctct tccggccggt aaaaagcgcg acgatccagc gtcatggacg
     9721 tggttcgccc cacctgacag taacggcagg cgtaggtgca caccttggac gggatgttgt
     9781 tgatccccag gctctttccc aaacgcctcg atggaacggg gccaaaagag tattgaaagc
     9841 cgcttgccat caccgttgtc tcccgcaggg atattgtcca tcgtcaaccc tgatattgca
     9901 tgggccgact tc
//
LOCUS       DAOOLJ010000064         9886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_7885_length_9886_cov_17.800631, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000064 DAOOLJ010000000
VERSION     DAOOLJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7885_length_9886_cov_17.800631"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLW16_08690"
     CDS             <1..186
                     /locus_tag="PLW16_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71024.1"
                     /translation="DYSPATAEAIDREVREIIGREYRRARDILADKRAVLLKGAQRLL
                     EQEKIDGEEIRVLLVET"
     gene            196..1263
                     /gene="mutY"
                     /locus_tag="PLW16_08695"
     CDS             196..1263
                     /gene="mutY"
                     /locus_tag="PLW16_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPB71025.1"
                     /translation="MQSKRQVLVRRLLDWYDRHHRPLPWRETSDPYRIWVSEIMLQQT
                     QVETVLPYYERFLERFPTVEALAAAKPDEVLRVWEGLGYYARARNLHAAARRLVERSG
                     GRVPETREALLALPGIGAYTAGAILSIAFGRRAASIDGNVRRVWSRIFASADGRTAPA
                     DLEDLAATIVPREAPGRFNQAVMDLGATVCTPRNPRCDGCPLRDLCPARAGGLQHVLP
                     AVKKKAPVPERDGTAGVVTDAAGRLLIVQRPAEGLLGSLWKFPGGFSRPGEPPEATLE
                     RTVREELGIRIRVGDRIGAVKQVYTHFRLTLHVFTGRIGRGRPRALACQDWAWAPLDT
                     LADRPFSGVDRKVMAVLAARR"
     gene            1288..1770
                     /locus_tag="PLW16_08700"
     CDS             1288..1770
                     /locus_tag="PLW16_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HPB71026.1"
                     /translation="MARDHIPVTPALVVMKREGVRYVLKSYAYVEHGGTRVSARELGE
                     DEHAIVKTLVMENERREPLIILMHGDRQVSTKALARTLGVKSIVPCDPKTAEKHTGYK
                     VGGTSPFGTRKPLPIYLEETIVGLPKLLINAGRRGLLAEMAPAELIRVLKPVPVRVAL
                     "
     gene            1848..2408
                     /gene="trxC"
                     /locus_tag="PLW16_08705"
     CDS             1848..2408
                     /gene="trxC"
                     /locus_tag="PLW16_08705"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HPB71027.1"
                     /translation="MKKDEMIIRCLNCGTKNRIPQDRLQARPACGRCHAPLDEMIIRC
                     LNCGAKNRMPETRLQDRPLCGKCGVPLVIAGTQGRPVDVTDADFSREVGAESGAVLVD
                     CWAPWCAPCRQVAPILDDLAAKYAGGVKIAKLNVDENPLTAAQYAIRSIPTMLLFKDG
                     KLVNRLVGALPKQEIEQHLLTIMQTN"
     gene            complement(2518..3060)
                     /locus_tag="PLW16_08710"
     CDS             complement(2518..3060)
                     /locus_tag="PLW16_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71028.1"
                     /translation="MPGMTRQILRLTAILLTALLCACAQTAPRPTAPPEAPVPSDPPV
                     LQEGLFNFSSEATDCFNRGLDFLENEGADPEGAKAREAFAELLTNYPRGKWQRAAAAL
                     VRLLEERTRLREGRAQDRQALEKARGETEQLRKELRALNDRLQVETTRLAQENEQLKK
                     DLQLLKELELQLDKRDRDLR"
     gene            complement(3148..4509)
                     /locus_tag="PLW16_08715"
     CDS             complement(3148..4509)
                     /locus_tag="PLW16_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPB71029.1"
                     /translation="MKLTISRKLLFSYVAMAVLTVLASAYALFSLQQLDRLSHAIIHE
                     DVFMVDLSKQMIDTVLDQESAEKKYLILKDPSIAEIFWNRSREFRRRIETWERKRNGT
                     DGKRRREVLALHERYDALFRTEEALVGENRLEEAVSLSGREGRVLVSNMVEVLRSLQR
                     ESNRDITGRLARMKSQAVRAGRMTLLLSGFSLLVGLLLAVLITTNISRPLKKLEQATD
                     RVADGEFDYDLRIDRKDEIGSLAYAFSDMMKRLKVMEEYNLDASPLTGLPGNRAIEKE
                     IERRLTARTAFSLCHVDLDNFKPFADHYGYAWGSEVIKEVARIMSEYEARHSDEKNFI
                     GHIGGDDFIIMARPDRVEAACRHLVTEFDRRMPKFYSDKDRQRGFIAAKDRQGVQQKY
                     PMLSVTIAIVTDDGTRFKDPLEMARVAAALKEYAKALPGSNYVRLEDVEAGVKGPEPG
                     VTT"
     gene            4691..5650
                     /locus_tag="PLW16_08720"
     CDS             4691..5650
                     /locus_tag="PLW16_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPB71030.1"
                     /translation="MAAKDLIRESVRVGAVKGWDGFIWMMKIVVPVSFLTTLLDRSGW
                     LARVDFLLQPAMGVLSLPPEAALPLLIGMLANIYGGIAAMVVLPLTLAQMTLVAIFLL
                     TAHNLIQEGIIQAKSGLHPLKATLFRIGAATVTVLCVAPFLDPGTATAAPAGGAAEAV
                     RPWIGVVEAWAWTTLRLAVKIFFIIFVIMILMEILKRTGWVHTIVKICAPFLRLMGLA
                     PKVGLLWMTAVIFGLAYGGAVIVEETRNGDLSRAELETLHLSIGINHSMVEDPILFMA
                     LGMSAFWLYIPRLIMAIVTVRLLNLWFRLTGPKQPAAVTPASR"
     gene            5811..7118
                     /locus_tag="PLW16_08725"
     CDS             5811..7118
                     /locus_tag="PLW16_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPB71031.1"
                     /translation="MIYNEEFETLPREVLEALQFKRLQQVLQRVYHTVGFYRRTFDAA
                     GVRPDDIKTLADLSRLPFTSKQDLRENYPFGLFAVPMSSVVRLHASSGTTGRSTVVGY
                     TKRDIDTWSELMARCFVAAGLTKNDIIHNAYGYGLFTGGLGAHYGAERLGASVIPMSG
                     GNTKRQIMILQDFGPTAICCTPSYALNLAEQGRAMGVDMRSLRLRVGIFGAEPWSDQM
                     RDQLEDQLAIQALNIYGLSEVMGPGVAMECSEGREGMHIFEDHFIVETIDPETGAILP
                     PGEEGEIVFTTLSKEAFPLIRYRTRDVARLITAPCRCGRSHVRMGRIMGRSDDMLIIR
                     GVNVFPSQIEAVLVGIEGLEPHYQLVVDREGTLDTLEVQVEVSERTFANADEVKVLQK
                     MERRIVKDIKDYLGVSARVKLVEPKSLQRFEGKASRVTDRRKL"
     gene            7150..7581
                     /locus_tag="PLW16_08730"
     CDS             7150..7581
                     /locus_tag="PLW16_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB71032.1"
                     /translation="MKVDQISIFLENKPGGLERVTRILKEANINIRTLSIADTSEFGI
                     LRLIVNDVEATVRVLKEDGFRVSRTTVVAVEVPDRPGGLYSIMEVLSAHGINVEYLYA
                     FVEKSGENAVIIFRFGNPDQAIDVLIKNNLTVLPGAKLYSL"
     gene            8113..8822
                     /locus_tag="PLW16_08735"
                     /pseudo
     CDS             8113..8822
                     /locus_tag="PLW16_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
     gene            8827..9735
                     /locus_tag="PLW16_08740"
     CDS             8827..9735
                     /locus_tag="PLW16_08740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPB71033.1"
                     /translation="METTIHRLINGDARELTYLDDASVHLIVTSPPYWNLKQYNDNAA
                     QLGHIQDYEAFLLELEKVWRHVYRILVPGGRLVCVVGDVCVARRDFGRHLVFPLHADI
                     CVICRRIGFDNLNPIIWHKIANASYEVENGSKFLGKPYEPNAIIKNDMEFILMQRKPG
                     GYRKPTNQQRQASRIEKDVFDRWFQQIWTITGASTKHHPAPFPLELATRLVRMFSFTE
                     DTVLDPFCGSGTTMVAALRTGRNSIGIEIDPEYCRMAARYLKAETSDLFTAAELRFEK
                     APTDAVAVISEEPALYGVRPAKKRLE"
BASE COUNT         1839 a         3169 c         3061 g         1817 t
ORIGIN      
        1 gactacagcc cggcgacggc cgaggcgatc gaccgggagg tccgggagat catcgggagg
       61 gagtaccgcc gggccagaga catcctggcg gacaagaggg cggtcctcct gaaaggggcg
      121 cagcggctct tggagcagga gaagatcgac ggcgaggaaa tccgggtgct gctcgtggaa
      181 acgtagacgc gaaatatgca gtcaaaaaga caggtgctcg tccggcggct cctggactgg
      241 tacgatcgcc atcaccggcc gttgccctgg cgggaaacgt cggaccccta ccggatctgg
      301 gtctccgaga tcatgctcca gcagacccag gtcgagaccg tccttccgta ttacgagcgc
      361 ttcctggaac ggtttccgac cgtggaggcc ctcgcggccg ccaagcccga tgaggtgttg
      421 agggtctggg aagggctggg ctactacgcg cgcgcccgga acctccacgc tgcggcccgc
      481 cggctcgtgg agcgcagcgg ggggcgggtg cccgagacgc gggaggcgct gctcgccttg
      541 cccgggatcg gcgcctacac cgccggggcg atcctgagca tcgccttcgg ccggcgggcg
      601 gcgtccatcg acggcaatgt tcgccgggtc tggagccgga tcttcgcgtc ggcggacggc
      661 cggacggcgc cggccgacct tgaggatctg gccgcgacga tcgtgccccg cgaggcgccc
      721 ggccgcttca accaggccgt gatggacttg ggggcgacgg tctgcacgcc gcggaacccg
      781 cgctgcgacg gctgccccct ccgggatctc tgtcccgcac gggccggggg gttgcagcac
      841 gtcctgccgg ccgtaaagaa gaaggccccc gttccggaac gggacggcac ggccggcgtc
      901 gtgaccgacg cggcgggccg gctactgatc gtccaacgcc cggccgaggg tctgctggga
      961 tcgctctgga agttccccgg cgggttttcc cggcccggcg agcctccgga agcgaccctg
     1021 gagcggacgg tccgcgagga gctgggaatc cggattcggg tcggcgaccg gatcggcgcg
     1081 gtgaagcagg tctataccca cttccggctc accctgcacg tcttcaccgg acggatcgga
     1141 cggggccggc cccgcgctct ggcctgccag gactgggcct gggccccgct cgatacgctt
     1201 gcggatcgcc ccttctccgg cgtcgaccgc aaggtgatgg cggtcctggc ggcgcggcga
     1261 tagccgccgg gcagaaggag ggaagccatg gccagggatc atattcccgt cacacccgcg
     1321 ctcgtggtca tgaagcgcga gggggtccgg tacgtcctga aatcctacgc ctacgtggaa
     1381 cacgggggca cgcgggtgtc cgcgcgagag ctgggggagg acgagcacgc catcgtcaag
     1441 acgctcgtca tggagaacga acgccgggag cccctgatca tcctcatgca cggcgaccgc
     1501 caggtgtcga ccaaggccct ggcgcggacg ctgggggtca agtccatcgt gccctgcgat
     1561 cccaagacgg ctgaaaaaca cacgggatac aaggtgggcg ggacctcccc tttcggcacc
     1621 cgcaaacccc tgccgatcta tctggaggag acgatcgtcg gcctgccgaa gctcctcatc
     1681 aatgcgggcc gccgggggct tctcgccgag atggcaccgg ccgaactgat cagggtcctc
     1741 aagcccgtgc ccgtccgcgt ggccctctga agcgcctggc gtcgccaggt atttcattga
     1801 cattttttcg cggttcggag aaaatggcgc catgcaaggg tgatgacatg aagaaagacg
     1861 agatgatcat ccgctgcctc aactgcggca caaaaaaccg catcccccag gatcgccttc
     1921 aggcacgtcc cgcctgcggg cgctgccacg cccccctcga cgagatgatc atccgctgcc
     1981 tcaactgcgg cgcgaagaac cgcatgccgg agacccgtct ccaggatcgt ccgctgtgcg
     2041 gaaaatgcgg cgtccccctc gtcatcgccg gcacccaggg acgccccgtg gacgtcaccg
     2101 atgcggactt ttcacgagag gtcggcgcgg agtccggggc cgtgctcgtg gactgctggg
     2161 cgccatggtg cgccccctgc cgccaggtgg cccccatttt ggacgatctg gcggccaaat
     2221 acgccggggg cgtcaagatc gccaagctca acgtcgacga aaacccgctg accgccgctc
     2281 agtacgccat ccgcagcatc ccgacgatgc tcctgttcaa ggacggcaaa ctggtgaacc
     2341 ggctggtcgg cgccctgccg aagcaagaga tcgaacagca cctgctgacc atcatgcaga
     2401 cgaactgaag acgtcgagac gtcccgacga tcggatccct ctgtacgacg ggaagacgcc
     2461 ggccgggggg agggggttct gcctccgcgg gcgcgacccg cggagggatc ccggcgttca
     2521 gcgaagatcc cggtcccgct tgtcgagttg caactccagc tccttgagca actggaggtc
     2581 cttcttcaac tgctcgttct cctgggcgag ccgggtcgtt tccacctgga gacggtcgtt
     2641 gagcgcccgg agctccttcc ggagctgttc ggtttccccc cgggcctttt ccaacgcctg
     2701 cctgtcctgc gcgcgccctt ccctgagccg cgtccgctct tcgagaagcc ggacgagggc
     2761 ggcggcggcc ctctgccact tgccgcgggg atagttggtc agaagctcgg caaaggcctc
     2821 ccgggccttc gcgccctccg gatcggcacc ctcgttttcc aggaaatcaa gaccccggtt
     2881 gaaacagtcc gtagcctccg aactgaagtt gaagagcccc tcctggagaa cagggggatc
     2941 gctcggaacc ggggcctcgg gcggcgccgt cggacggggc gcggtctgag cgcaggcgca
     3001 cagaagcgcc gtcagcagga tcgccgtcag gcggagaatc tgtcgagtca tgcccggcat
     3061 ggaagctgtc cttaccgttt cccgcgcctg ctcccggaga ggggcgcagt cagatgaggg
     3121 atgcttacgg gacgcagcat ccggggttca tgtcgtcacg ccgggttccg gccccttcac
     3181 accggcttcc acgtcctcca ggcgaacgta gttgctgccg gggagggcct tggcgtactc
     3241 tttcaacgcc gccgccaccc gcgccatctc caggggatcc ttgaaccgcg tgccgtcgtc
     3301 cgtgacgatg gcgatggtaa cgctcagcat ggggtacttc tgctgaaccc cctggcggtc
     3361 cttggcggcg atgaatccgc gctgccgatc cttgtcgctg tagaacttcg gcatccgccg
     3421 gtcgaactcg gtgaccagat gccggcaggc cgcttccacc cggtcgggcc tggccatgat
     3481 aatgaagtca tcccccccga tgtgtccgat gaagttcttt tcatcgctgt ggcgggcttc
     3541 atattccgac atgatgcggg ccacctcctt gatgacctcg ctgccccagg catagccgta
     3601 atgatcggcg aagggcttga aattgtcgag atcgacgtgg cacagggaaa aggccgtccg
     3661 ggccgtgagg cgccgctcga tctctttttc aatggcccgg ttgcccggca ggccggtcag
     3721 ggggctggcg tcgaggttgt actcctccat gaccttgagc cgcttcatca tgtcgctgaa
     3781 ggcgtaggcg aggctgccga tctcgtcctt acggtcgatg cgcagatcat agtcgaactc
     3841 gccgtccgcc acccggtccg tcgcctgttc gagcttcttc aggggccggg agatgttggt
     3901 ggtgatcagc acggcgagca gcaggccgac cagcaagctg aacccgctca acagcagggt
     3961 catccggccg gcgcggacgg cctgggattt catccgggca aggcggccgg tgatgtcccg
     4021 attcgactca cgctgcagcg accggagaac ctccaccata ttcgagacga ggacccgacc
     4081 ctcccggccc gataacgaga ccgcctcctc caggcggttc tcgccgacga gggcctcttc
     4141 ggtccggaag agcgcgtcgt agcgttcgtg cagggccaga acctcccggc gccgcttgcc
     4201 gtccgtcccg ttccgttttc gctcccacgt ctcgatccgc cgccggaact cccgacttcg
     4261 gttccagaag atctcggcga tggagggatc cttgagaatc agatattttt tctcggcgct
     4321 ctcctgatcg aggaccgtat cgatcatctg cttgctgaga tcgaccatga aaacatcttc
     4381 atggatgatg gcgtgactga gacgatcgag ctgctgaagg ctgaaaaggg cataagcgct
     4441 ggccagaacg gtcaaaaccg ccatggcaac ataactgaac agcaacttac ggctgatggt
     4501 gagcttcatc gatcgggcat atccctttga gcttcgggtg tttttttagc atatgcgccc
     4561 tgccgtgtaa acgggaattt gtttgcggga ccctgttttt tgccttcacc gcaccgggct
     4621 tttctgctaa acagaggccg cgtcacgaca gccggtttcc gggcggcggg ggaggcagcg
     4681 gtcaagcttc atggcagcaa aagatctgat tcgggaatcg gtgcgcgtcg gcgcggtcaa
     4741 gggctgggac gggtttatct ggatgatgaa gatcgtcgtt cccgtctcct tcctgacgac
     4801 gctcctggac cggagcggct ggctggcgcg ggtcgatttt ctcctgcagc cggccatggg
     4861 cgttctgagc ctgccgccgg aagccgccct gccgctcctg atcgggatgc tggccaacat
     4921 ctacggcggg atcgcggcca tggtggtgct gcccctgacc ctcgcccaga tgaccctggt
     4981 cgccatcttc ctcctgacgg cccacaacct gatccaggag gggattatcc aggccaaatc
     5041 gggccttcac ccgctgaagg ccaccctctt ccgaatcggc gccgccacgg tgaccgtttt
     5101 gtgcgtggcg ccgtttctcg accccgggac cgcgacggcg gccccggccg ggggggcggc
     5161 ggaggccgtc cgcccctgga tcggggtcgt ggaagcctgg gcctggacaa ccctccggct
     5221 cgcggtgaag atcttcttca tcatcttcgt catcatgatc ctgatggaga tcctgaagcg
     5281 caccggatgg gtccacacga tcgtgaagat ctgcgccccg tttctccggc tcatggggct
     5341 cgcgcccaag gtcggcctgc tgtggatgac ggccgtcatc ttcggcctgg cctacggcgg
     5401 cgccgtgatc gtcgaggaga cgcgcaacgg ggacctctcc cgggcggagt tggaaaccct
     5461 gcacctgtcc atcgggatca accactccat ggtcgaggac ccgatcctgt tcatggccct
     5521 cggcatgagc gccttctggc tctacatccc ccggctgatc atggcgatcg tgacggtccg
     5581 cctgctgaac ctctggtttc gcctgaccgg accgaagcag cccgccgcgg tcacgcccgc
     5641 gtcccgctaa gtatccactt ctggtcgcct tttcatgaat gttccttgtc cccggatgga
     5701 aattctgtta taggttcgac cggtggtcgc cggaagccga tccctgaggt gcagggagcc
     5761 gatttcaaga acgcccggca tgacaaatcc atgaggaggg gatggctgtt atgatctata
     5821 atgaagaatt cgagaccctg ccccgggaag tcctggaggc cctgcagttc aagcgcctcc
     5881 agcaggtgct ccagcgggtc tatcataccg tcggtttcta ccggcgcacc ttcgacgccg
     5941 ccggcgtaag gcccgacgac atcaagaccc tggcggacct ctcccgcctg cccttcacgt
     6001 cgaagcagga tctccgggag aactacccgt tcggcctctt cgccgtcccc atgagcagcg
     6061 tggtccggct gcacgcctcg tccggaacga ccggccgttc gacggtcgtc ggctacacca
     6121 aacgcgacat cgacacctgg tcggaactga tggcccgctg cttcgtcgcc gcgggactca
     6181 cgaagaacga catcatccac aacgcctacg gctatggcct gttcaccggc ggcctcggcg
     6241 cccactacgg ggcagaaagg ctgggcgcca gcgtgatccc gatgtccggc ggcaacacga
     6301 aacggcagat catgatcctg caggacttcg gtccgacggc catctgctgc acgccgtcct
     6361 acgcgctcaa cctggcggaa cagggtcggg ccatgggggt ggacatgcgg tcgctccgcc
     6421 tgcgcgtcgg gatcttcggg gccgagccct ggagcgacca gatgcgcgac cagctcgaag
     6481 atcagctcgc catccaggcc ctgaacatct acgggttgtc ggaagtcatg gggcccggcg
     6541 tggccatgga atgctccgaa ggccgggagg ggatgcacat cttcgaggac cacttcatcg
     6601 tggagaccat cgatcccgag acgggcgcca ttctgcctcc gggggaggag ggggagatcg
     6661 tcttcacgac gctcagcaag gaggccttcc ccctgatccg ctaccggacc cgggacgtcg
     6721 cccgcctgat caccgccccc tgccgctgcg gccggagcca tgtccgcatg ggccgcatca
     6781 tggggcgcag cgacgacatg ctcatcatcc gcggcgtcaa cgtcttcccc tcccagattg
     6841 aggcggtcct ggtcggcatc gaaggcctgg aaccccacta ccagctcgtc gtcgaccggg
     6901 aaggcaccct ggacaccctg gaggtgcagg tcgaggtcag cgagcgcacc ttcgcgaacg
     6961 ccgacgaggt gaaggtcctg cagaaaatgg aacggcgcat cgtcaaggac atcaaggact
     7021 acctgggcgt ctccgccagg gtgaagctgg tcgaacccaa atcgctgcag cgcttcgaag
     7081 gcaaggcgag ccgggtcacg gatcggcgca aactttaacg tctcttttaa cccgaggagg
     7141 tcggtgccaa tgaaagtcga tcagatttcc atctttctgg aaaacaagcc gggcggactg
     7201 gagcgggtga cccggattct caaggaggcc aacatcaata tccggaccct gtcgatcgcc
     7261 gacacctccg agttcggcat cctgcgcctc atcgtcaacg acgtggaggc caccgtgcgc
     7321 gtgctcaagg aggacggctt ccgggtcagc cgaacgaccg ttgtcgccgt cgaggtgccg
     7381 gaccggccgg gaggtttgta cagcatcatg gaggtcctgt cggcccatgg catcaacgtc
     7441 gaatacctgt acgccttcgt cgagaagagc ggcgagaacg ccgtgatcat cttccgcttc
     7501 ggcaatcccg accaggcgat cgacgtcctc atcaaaaaca acctgaccgt gctgccgggc
     7561 gcgaagctct attcccttta ggaggtgtgc cgggaccgcc gggacggggg cctctgccga
     7621 ccgccccgtg cgggggaatg gttggattct caacgctccg cttacactcc atatcaaccg
     7681 gtgccccggc gcgaggggac ggcttatgtc ccccgcaatt tcttccaacg cccgatcttc
     7741 gaggatgcgc aatgaaagcc gcaagccgcc ttaggtccgg tcgtcgctcg ccacgaatcc
     7801 ggttccttcc tcgatcggcc gccgggcgtc cggtaagcgc cggaggaccg gcaaccgtcc
     7861 cccacccgtc actattaata gaggctctaa aaaagcaagc aaatgaagcc acacacgcag
     7921 ctaaagctgc ggataaatct tgcattaaag tatttggcgc accgtagtaa ttgtaccgac
     7981 aaacatgcgc acgttagttc cctgtatgat taaatgatcg taaaacaaag gcaatacaga
     8041 tgatcgctac gttccggctg atttctgcgt tcgcgtgttg aataggtgcg aggaatctga
     8101 tacggcatgg ccatgaatcg tgacgacatc ctgcgtaatt tgccggtgca taccgggagg
     8161 gcagtcgccc actactggaa gactcggacg ggtcagcaag atagacagcg ggaaaccggc
     8221 aagacggacc agggcctgcg aagcgccgtc accggcggtg cgcagatgga cggcttcgtc
     8281 gatctcttca cggaactgat tacggcggcc gggatttcgg aacggcatgt cttcagaaag
     8341 aaggccgtca aattgccagg cttcttccgc ccgaccaagg agtgggacct gctcgtagtg
     8401 cgcgagaggc caagtcccag gtcggtcctt ccttcggcaa taacttcaac aaccggacgg
     8461 aagaggcgat aggaagcgcc cttgacctct ggaccgcata ccgtgaacgt gcctacctca
     8521 gcagccccca gccttttctc ggttactttt tcatgctgga ggaccgcgct gcctccaacc
     8581 gcccggtcaa ggtgaaggag ccgcatttca aagtctttcc cgaattcgtc ggcgcttcct
     8641 acatgcgccg ctatgaaatc ttctgccgca agctcgtgct ggaaaggcat tacacggtgg
     8701 cggcgttcat ttcgtccaca atagaggaag ggcttcaagg aagcttctcg accccggcgg
     8761 ccgacctttc tgtcgagcat ttcgcgaaga ccctcacggc ccacttggcc gcctttgcgt
     8821 gaactcatgg aaaccaccat tcacaggttg atcaatggtg atgcgaggga acttacgtac
     8881 ctcgacgatg cctcggttca tcttatcgtc acatctcccc cttactggaa tttgaaacaa
     8941 tacaacgata acgcggccca actcggacat attcaggact acgaagcatt cctgctcgag
     9001 ttggaaaagg tttggcgcca tgtttacaga atcctcgtgc ccggggggcg cctcgtttgc
     9061 gtggtcggag atgtttgtgt ggcgcgtcgc gactttggac gccacctcgt ctttccttta
     9121 cacgccgaca tttgcgtcat ctgccgaagg attggattcg acaacctgaa cccgatcatc
     9181 tggcacaaaa ttgcaaacgc ctcctacgag gttgagaacg gatcaaaatt cttggggaag
     9241 ccctacgagc cgaacgcaat tatcaagaac gacatggaat tcatcctgat gcagcgcaaa
     9301 cccggcgggt accgaaaacc caccaaccag cagcgccagg caagccggat tgaaaaagat
     9361 gtttttgacc gatggtttca gcaaatctgg accatcaccg gggcctcaac caaacaccac
     9421 cccgcccctt tcccgctgga actggccacc cgcctcgtcc gcatgttctc cttcaccgag
     9481 gatacggttc tggatccgtt ctgcggatcg ggcacgacga tggttgccgc tctgcgcacg
     9541 ggacgtaata gcattggaat cgaaattgat ccggaatact gtcgcatggc ggcccggtat
     9601 ctgaaagcgg agacatccga ccttttcacg gcagcggaac ttcgctttga gaaagcgccc
     9661 acggacgccg ttgccgtcat aagtgaagaa cccgccctat acggagtccg gccagccaag
     9721 aagagacttg aataggcgaa cagtgcgctc cacacggatc gccgagaact ttcttcatct
     9781 caccttcgag aaagcgtccc gaagaacgtc ttcggcgggt ctggttttga gctttccttc
     9841 acggcagtcc cgatagcgac gctcggcttc ctgaagccag agttct
//
LOCUS       DAOOLJ010000065         9792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_8018_length_9792_cov_18.382561, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000065 DAOOLJ010000000
VERSION     DAOOLJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_9792_cov_18.382561"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLW16_08745"
     CDS             complement(<1..224)
                     /locus_tag="PLW16_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPB71034.1"
                     /translation="MDAGSVRKALLLAFGILVMSGAVPAVAAQKISVFVSIAPQVYLV
                     ERIGGERVAVEALVGPGREPHDYEPTPRQMA"
     gene            complement(344..1249)
                     /locus_tag="PLW16_08750"
     CDS             complement(344..1249)
                     /locus_tag="PLW16_08750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB71035.1"
                     /translation="MLNFLPKPLVGVIAVSLLVLNILVWCLFLFAVALIKFLLPFKPV
                     RRAVDPILNAIATTWISGNNGWMRLTQKTRWDIQGLEGLQYRGWYLVSSNHQSWVDIF
                     VLQNMFNRRIPFLKFLIKQELLYVPIMGLAWWALDYPFMKRYSAEYLKKHPEMRGKDL
                     ETTRKACKKFSLIPTSVMNFPEGTRFTPAKHDRQESPYRHLLKPKAGGVALVLDAMGE
                     KFQSLLNVTIVYPDGIPGFWEFLSGRVKRITVRVQNLPIPKHFIGGDYENDAQFRAAF
                     QQWLHEVWLEKDALIAQLRAETAPA"
     gene            complement(1437..1937)
                     /locus_tag="PLW16_08755"
     CDS             complement(1437..1937)
                     /locus_tag="PLW16_08755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPB71036.1"
                     /translation="MMRRRYGLGRIGLVLAGLLLCVGCGEPQRPGVTGAPPDFTLKDL
                     QGRKWTLGDQLGKPVLMLFTTTWCPSCRQEIPHYKAIYDHYGPRGLEMVNIDIQESRE
                     KVSRFADGHGLPYRILLDETGEAAQAYGVVGVPFAALLDRDGQAVCLPCPNLDSELAA
                     LYPAAP"
     gene            complement(2066..2776)
                     /locus_tag="PLW16_08760"
     CDS             complement(2066..2776)
                     /locus_tag="PLW16_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPB71037.1"
                     /translation="MPLLEGLNTFLEGAPALAYLAVFAGGVLVSFTACVYPVVPITIA
                     FIGARSAGSRTRGFSLSLVYVLGMALTYTALGGIAALSGKLFGQLQTNPWTYFVMANI
                     CILMGLAMLEVFILPVRTPAFIARLQPRGKTRGLAGSLAVGAASGLVLGPCTAPVLAV
                     LLSYVATRQHLLFGMSLLFVFALGMGVLLVIVGTFAGLLANLPKSGVWMARVNRLFGW
                     VLIGTGEYFLIVAGQLWI"
     gene            2944..4434
                     /gene="glpK"
                     /locus_tag="PLW16_08765"
     CDS             2944..4434
                     /gene="glpK"
                     /locus_tag="PLW16_08765"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPB71038.1"
                     /translation="MRYILALDQGTTSSRAILFDRAGAVRAVAQKEFRQIYPKPGWVE
                     HDPTEIWATQIGVAAEAMTTAGISGADIAAIGITNQRETTVLWDRRTGQPVYNAIVWQ
                     DRRTADLCDALKAEGLTAVFRRKTGLIIDAYFSGTKIRWILDHVPGARARAEAGELAF
                     GTIDTWLVWNLTRGARHVTDASNASRTLLYNIHTGAWDDDLLRMLDIPRAVLPEVRAS
                     SDAVGETAPSVLPGGIPIAGIAGDQQAALFGQMCTEPGLVKNTYGTGCFMLMNTGDKP
                     ILSENNLLTTVAWKIGSEIRYALEGSVFMGGAVVQWLRDGLGIIRTSAAVEELANRVP
                     DNGGVYLVPAFAGLGAPYWDPYARGTLVGLTRGSGASHIARAALESIAYQSLDVLECM
                     ATDAGIDIRELRVDGGATANDTLMQFQADILGVPVIRPVIAETTAMGAACLAGLAVGY
                     WRDLAELNGRLQVDRRFEPAMDRARAGALVREWRRALERSRRWIEK"
     gene            4454..6046
                     /locus_tag="PLW16_08770"
     CDS             4454..6046
                     /locus_tag="PLW16_08770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPB71039.1"
                     /translation="MTAKDPTHRQTLLAALAAKGAWDVIVIGGGATGLGVAVDAAARG
                     YRTLLLEQHDYSKGTSSRSTKLVHGGVRYLKQGNVSLVLEALHERGLLMFNAPHLIRH
                     QAFIVPSYGWWEGPFYGVGMKVYDKLAGKLGLEPSQRLSLRETRRRIPTLEPDGLRGG
                     VIYYDGQFDDARLAVNLAQTVIDRGGTALNYMPVTALLKKGDAVSGVAARDEETGRVY
                     EIPGRVVVNATGVFADGVIRMDDPTVPNLIAPSQGVHLIVGREFLPGHAAIMVPHTDD
                     GRVLFAVPWHDKVVLGTTDTPIERADLEPRVLEEEIDFILKHSRRYLARGPERKDVLS
                     MFAGIRPLFRTGDDKSTAELSRDHTLLVSPSGLVTITGGKWTTYRRMAQDTVDLAITV
                     GGLKRRPCPTEQMPIHGWVEASDDADPLHAYGADAPGIRRLIAARPELGKRLHGALPY
                     LRAEVVWGVRREMARTVEDVLARRTRALFLNARASVEMAKDTAALMAAELGRDEDWQA
                     EQVARYKALAAGYLPSLKGGPI"
     gene            6043..8556
                     /locus_tag="PLW16_08775"
     CDS             6043..8556
                     /locus_tag="PLW16_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPB71040.1"
                     /translation="MNAAMQPKFAIKAPRGLSPRIAWLRDYYFQGTARAWNNEATAWT
                     TGTPWDVVYDEITYYIVPETYPFLQTFRSALRQAARPVPLPEGFWKWSLPERRAWFIR
                     EVMVNHVPQELLPGDLLAGGRFNLQASRCWTKAETAARDRLIYGRKGARAVMKAFHDH
                     GYGNSGATSGHLIPDYARVLDIGWRGIHADLAETYDRLTERDRKGARGAQLRAMMTAA
                     GMPRDLAARYAVLCTRRAAGEADPARRDELAQMAANLERVPWEPPRTFWEAVQALWLT
                     HMLVMSDENYPGPGVSFGRLDQSLYPFWQRSLDEGMDREFGKEILRCLWVHCNTVYDM
                     MIRVGGNQGITAGYGQLFNLSGMGPGGRDMTNDLTYALLEVIDEMTPILEPKPNVRLH
                     RNSPEALLDRVVDMIASSQGAPFLLNFDERSMAGMLREAKEAGVTHLIHEGNVHDYAS
                     VGCLENTMVGNDRSGTVDNNVNLVKAVELALTGGRDLVPFVDPMNGKTERIRQAGPRT
                     GDATAFATWDAFWEAYCAQTRYILARCAETYEASEAIRARFSPTPYLSCLVKGCAGQG
                     RDVTQGGAELSFTTMEGVTFATTVDSLLAIKYLVYDQKACTMAELIRALKDNWAGHEI
                     LQAKAKFRAPKYGRDDDEADRMAARVMELWAAETWKFRTRSTGRRFRPGMLSWNYWVG
                     DGFIVAATADGRPRGQFLSNAICPSNGADIHGPTANVNSVGKALGGKAPSGRGDWEAY
                     LCNLPNGASHTMTFSPSLLRDPEHRRKFRAFLKGYIENGGTALQINLIDADMLREAQK
                     HPRDYRHLLVRVTGYNAYFTSIGRELQDEIIARESHARY"
     gene            8566..9570
                     /locus_tag="PLW16_08780"
     CDS             8566..9570
                     /locus_tag="PLW16_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPB71041.1"
                     /translation="MTATAAPVDESTESLILNIQRMSTEDGPGIRTTVFFKGCNLHCL
                     WCHNPESLSARPQMQWIETRCIGCKTCLAACPEGALSDDGAGIRILRDRCTACGACSR
                     ACPALALERLGEPWRLDRLLDEVEKDRAYFEASGGGVTVSGGEPALQAPFVAALLAGC
                     RQRGLHTALDTCGFCATGALEGLLPVTDLVLYDLKEIDPERHRRFTGQSNERIFANLL
                     LVRDRMRAQERPARLWLRTPIIPRMTAREDNIAGLGRFIVENLADVVERWELCAFNNL
                     CRDKYRRLDMDWACREDALMTAGEMEALDDAARRVGVAAARVVWTGAVRPDEPAGDPV
                     "
     gene            9603..>9792
                     /locus_tag="PLW16_08785"
     CDS             9603..>9792
                     /locus_tag="PLW16_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71042.1"
                     /translation="MTDLQTRFLPEDREAFASDVKVGLLATVSGEGWPHLTLITTLET
                     ASPTELTWGQFCEGASKRH"
BASE COUNT         1682 a         3302 c         3333 g         1475 t
ORIGIN      
        1 gccatctggc ggggggtggg ttcgtaatcg tggggttccc gtcccggacc gacgagcgcc
       61 tcgacggcca cccgctcgcc gccgatccgt tcgaccagat agacctgggg cgcgatgctg
      121 acaaataccg atattttctg tgcggcgacg gccggaaccg cgccgctcat gaccaggatc
      181 ccgaaggcca gaagcagggc cttccggaca gaaccggcgt ccatctcgat gctatccctt
      241 catgcgcaac gccgttgcgc gttttttctt ccggccgccc gcgcctctgc ccccccggtc
      301 cgaaagaccg ccgggcggca acggcccctt cggcctttcc ccgtcaagcc ggggcggtct
      361 cggcgcgcag ttgcgcgatc agggcgtcct tctccagcca cacctcgtgc agccactgct
      421 ggaacgccgc gcggaactgc gcgtcgtttt catagtcacc gccgataaaa tgcttcggaa
      481 tcggtaaatt ctgcacgcga accgtaatcc gcttcacccg cccggagaga aattcccaga
      541 atcccgggat gccgtcgggg taaacgatgg tgacgttcaa cagagactgg aatttttcgc
      601 ccatcgcgtc caggaccagg gcgacgcctc ccgccttggg tttcagcaga tggcgatagg
      661 gggattcctg ccggtcgtgc ttggccggcg tgaaccgcgt cccctcggga aagttcatga
      721 cgctggtggg gatcagcgag aactttttac aggccttgcg cgtggtttcg agatccttgc
      781 cccgcatttc ggggtgcttc ttgagatatt ccgcactgta ccgcttcatg aacgggtaat
      841 ccagcgccca ccaggcgagc cccataatcg gaacataaag caattcttgc ttgatgagaa
      901 acttgaggaa cgggatccgg cgattgaaca tattctggag cacgaagatg tccacccagc
      961 tctggtggtt gctgctcacg agataccagc cccggtattg gagtccttcg aggccctgga
     1021 tgtcccaccg ggttttctgc gtcagcctca tccagccgtt gttgccggaa atccaggttg
     1081 tggcgatggc gttcaggatg gggtcgaccg cccggcgcac cggtttgaag gggaggagga
     1141 acttgatcag ggcgacggcg aacaggaaca ggcaccagac aaggatgttc agaacgagca
     1201 gcgagacggc gatgacgccg accaagggtt tcggcagaaa gttcagcatg atggggctcc
     1261 cggtcccgga ttttcgtgat agtgcgaaac gcggactata ggaggagacg gccggcatgt
     1321 caatcaaaaa cagcgcaggc gtgcacgaaa agcgtttcgg gcgtcaggac ggacggcggt
     1381 gtgtcgggca gcggtcggct ccccttcacg ggcgcccggc gggacccctg ccgggcttac
     1441 ggggccgcgg gatagagggc ggcgagttcc gagtcgagat tggggcaggg caagcagacg
     1501 gcctgtccgt cccggtcgag gagggcggcg aaggggaccc cgacgacacc gtaggcctgg
     1561 gcggcctcgc cggtttcgtc gagaaggatg cggtagggca gcccgtgccc gtcggcaaag
     1621 cgggaaacct tctcccgcga ttcctggatg tcgatgttca ccatctccag gccccgggga
     1681 ccgtagtggt cgtaaatcgc cttgtaatgg gggatctctt ggcggcagga ggggcaccag
     1741 gtggtcgtga agagcatcag gacgggcttg cccagctgat cccccaaggt ccacttgcgg
     1801 ccctgaaggt ccttcagcgt gaagtccggg ggggcgcccg tgaccccggg ccgttgcggc
     1861 tcgccgcacc cgacacacag gagcagaccc gccagcacga gcccgatcct ccccaagccg
     1921 tatcgtcttc tcatcattct ccgtttctcc tcccccgttg tttcccctcg ccgaatccgt
     1981 cgaccgtccc ggcgcgcggt ccgctctgta tccgcgcctc cctgcggcgc gcatccttcc
     2041 cttgtccacg tcccaacgcc cggcgctaaa tccagagctg accggcgacg atcaggaagt
     2101 actccccggt cccgatcagg acccagccga agaggcgatt gacgcgcgcc atccagacgc
     2161 ccgatttggg caggttggcc agcagtccgg caaaggtccc aacaatgacg agcaggaccc
     2221 ccatgcccag ggcgaagacg aacagcagac tcattccgaa cagcaggtgc tgtcgcgtgg
     2281 cgacataact caacagcacg gccaggaccg gagcggtgca cggtcccaga accagccccg
     2341 acgcggcgcc gacggcgagg ctgcccgcga gaccccgggt tttcccgcgc ggctggagac
     2401 gggcgatgaa ggccggggtg cgcacgggca ggatgaagac ctccagcatc gcgagcccca
     2461 tgaggatgca gatgttggcc atgacgaaat aggtccaggg gttggtctgg agctggccga
     2521 agagctttcc ggacaaggcg gcgatcccgc ccagggccgt gtaggtgagg gccatcccca
     2581 ggacgtagac gagcgacagg gaaaatcccc gggtccggga gcccgcgctg cgcgccccga
     2641 tgaaggcaat ggtgatgggc accacgggat agacgcaggc ggtgaagctc accagcaccc
     2701 cgcccgcgaa tacggcgaga tacgccaggg cgggagcgcc ctccaggaat gtgttcagtc
     2761 cctccagcag cggcatggtc cgtctccttt tttcccgggg gcccatcccc ggccgggggc
     2821 gggcgtcaag gtctggcgat atgtaccaca tcccgccggc ggatgccagg ggcgagttaa
     2881 atttcccttg tcattgtgag gaattgcatg caataatgcc gtatcctgtc gaaggaggtc
     2941 cgtgtgcggt acatcctggc cctcgatcag ggcacgacga gttcccgagc catcctcttc
     3001 gatcgcgccg gcgccgtgcg ggccgtcgcg cagaaggaat tccgccagat ctaccccaag
     3061 cccggctggg tggagcacga tccgacggaa atctgggcca cccagatcgg ggtagccgcc
     3121 gaggcgatga cgacggcggg gatctccggc gcggacatcg ccgcgatcgg catcaccaac
     3181 cagcgggaaa cgacggtcct ctgggaccgc cgcaccgggc agccggtgta caacgccatc
     3241 gtgtggcagg accgccgcac cgccgacctc tgcgacgccc tgaaggcgga ggggctgacg
     3301 gcggtcttcc ggcggaagac cggcctcatc atcgacgcct atttttcggg cacgaaaatc
     3361 cgctggatcc tggatcacgt ccccggcgcc cgcgcgcggg cggaggcggg cgaactggcc
     3421 ttcggcacga tcgacacgtg gctcgtctgg aacctcaccc ggggcgcccg gcacgtaact
     3481 gacgcctcca acgcctcccg gaccctgctc tacaacatcc acaccggcgc ctgggacgac
     3541 gatctcctga ggatgctcga cattccccgt gccgtcctgc ccgaggttcg ggcgtccagc
     3601 gacgcggtcg gcgagacggc cccgtccgta cttcccggcg gcatccccat cgcgggcatc
     3661 gccggcgatc agcaggccgc cctcttcgga cagatgtgca ccgagcccgg cctggtgaaa
     3721 aacacctacg gcacgggttg tttcatgctg atgaatacgg gcgacaagcc gatcctgtcg
     3781 gaaaacaacc tcctgacgac ggtggcctgg aagatcggtt cagagatccg gtacgccctc
     3841 gaggggagcg ttttcatggg cggcgccgtg gtgcagtggc tccgggacgg cctggggatc
     3901 atccggacct ccgccgcggt ggaggaactg gccaaccgcg tgcccgacaa cggcggggtc
     3961 tatctggtgc ccgccttcgc cgggctgggc gccccctact gggacccgta cgcccgcggg
     4021 accctggtcg gcctcacccg gggctccggc gcgtcccaca tcgcccgggc ggccctggag
     4081 agcatcgcct accagtccct cgacgtcctg gaatgcatgg cgacggacgc cgggatcgac
     4141 atccgcgagc tgcgcgtcga cgggggggcg acggccaatg atacgctgat gcagttccag
     4201 gcggatatcc tgggcgtccc ggtgatccgc cccgtgatcg ccgaaaccac cgccatgggc
     4261 gcggcctgcc tggcggggct ggcggtcggc tactggcgag atctggcgga actcaacgga
     4321 cggctccagg tggaccggcg gttcgagccc gccatggacc gcgcccgggc gggagcgctc
     4381 gtgcgcgagt ggcgtcgggc cctcgaacgg tcgcggcgct ggatcgaaaa atagctcaag
     4441 gggtgatgga cctatgacgg caaaagaccc gacccatcgg cagaccctcc tggccgcgct
     4501 ggccgccaag ggggcctggg acgtcatcgt catcgggggg ggtgccaccg gcctgggcgt
     4561 ggcggtcgat gcggcggcgc gggggtaccg gacgctcctc ctggagcagc acgactattc
     4621 gaaaggaacc tccagccgca gcaccaaact cgtccacggc ggcgtccgct acctgaagca
     4681 gggaaacgtc tcgctcgtcc tggaggccct ccacgagcgc ggcctgctga tgttcaacgc
     4741 cccccacctg atccgccacc aggccttcat cgtccccagc tacggctggt gggaaggccc
     4801 gttctacggc gtcggcatga aggtctacga caagctcgcg ggcaagctgg gcctggaacc
     4861 ctcccagcgg ctctccctcc gggagacgcg ccggcgcatt ccgaccctgg agcccgacgg
     4921 cctccggggc ggggtcatct actacgacgg gcagttcgac gacgcgcgtc tggcggtcaa
     4981 tctagcccag accgtcatcg accgcggcgg cacggccctg aactacatgc cggtcacggc
     5041 cctgctcaag aaaggcgacg cggtgagcgg ggtcgccgcc cgggacgagg aaacgggccg
     5101 ggtgtacgag attccgggcc gggtcgtggt gaacgccacc ggcgtcttcg ccgacggcgt
     5161 catccggatg gacgacccga cggtgccgaa cctgatcgcc cccagccagg gggtgcacct
     5221 gatcgtcggc cgggaattcc tgccggggca cgccgccatc atggtgcccc atacggacga
     5281 cggccgcgtc ctgttcgcgg tcccctggca cgacaaggtc gtcctcggca cgacggacac
     5341 cccgatcgag cgggcggacc tcgagcccag ggtcctcgag gaggagatcg atttcatcct
     5401 gaagcactcc cggcgctacc tcgcccgggg cccggaacgg aaggatgtcc ttagcatgtt
     5461 cgccggcatc cggcccctct tccggacggg ggacgacaag agcacggcgg agctgtcgcg
     5521 cgaccatacg ctcctggtct cgccgtcggg gctggtcacg atcacgggcg gcaaatggac
     5581 gacgtaccgg cggatggccc aggacacggt ggatctggcg atcacggtgg gcgggctgaa
     5641 gcggcgcccc tgccccaccg agcagatgcc catccacggc tgggtcgagg cgtccgacga
     5701 cgccgatccc ctccacgcct acggcgccga cgccccgggc atccggcggc tcatcgccgc
     5761 ccggccggag ctgggaaagc gcctccacgg ggcgctcccc tacctccggg ccgaggtggt
     5821 ctggggcgtc cgccgggaga tggcccgcac ggtggaggat gttctggccc ggcgcacgcg
     5881 ggcgctgttt ttgaatgcac gggcgtcggt cgagatggcg aaggacacgg cggccctcat
     5941 ggccgcggaa ctcggccggg acgaagactg gcaggcggag caggtcgccc gttacaaggc
     6001 gctggcggcc ggttatctgc cctccctgaa aggaggcccc atatgaacgc ggcgatgcag
     6061 ccgaaattcg ccatcaaggc gccccggggg ctctcccccc ggatcgcgtg gctgagggat
     6121 tactacttcc aggggacggc gcgcgcctgg aacaacgagg ccacggcctg gacgaccggg
     6181 acgccctggg acgtcgtgta cgacgagatc acgtactaca tcgtcccgga gacctacccc
     6241 tttctccaga ccttccgctc cgccctgcgg caggccgccc ggcccgtccc gcttccggag
     6301 gggttctgga aatggtccct gccggagcgg cgcgcctggt tcatccggga ggtgatggtg
     6361 aaccacgtcc cgcaggagct cctccccggc gaccttctgg ccggcggccg cttcaacctg
     6421 caggcgtccc gctgctggac gaaggcggag acggcggcgc gggaccgcct gatctacggc
     6481 aggaagggcg cccgcgccgt catgaaggcg ttccacgacc acggctacgg gaacagcggg
     6541 gcgacgagcg gccacctgat ccccgactac gcccgagtgc tcgacatcgg ctggcggggc
     6601 atccacgcgg atctcgcgga aacgtacgat cgtctgacgg agcgggacag gaaaggcgcc
     6661 cgcggggccc agctccgcgc catgatgacg gcggccggga tgccccgcga cctggccgcg
     6721 cgctatgccg tcctctgcac gcggcgggcg gcgggcgaag cggacccggc gcggcgggat
     6781 gaactggccc aaatggcggc caacctcgag cgggtcccct gggaaccgcc ccggaccttc
     6841 tgggaggcgg tgcaggccct gtggctcacg cacatgctcg tcatgagcga cgagaactac
     6901 cctggacccg gcgtgtcctt cggcaggctc gaccaatccc tgtatccctt ctggcagcga
     6961 tccctcgacg agggcatgga ccgcgagttc ggcaaggaga tcctgcggtg cctctgggtg
     7021 cactgcaaca cggtctacga catgatgatc cgcgtggggg gcaaccaggg catcaccgcc
     7081 gggtacgggc agctcttcaa cctctccggc atgggcccgg gagggcggga catgaccaac
     7141 gacctgacct acgccctgct cgaggtcatc gacgagatga cccccatcct ggagcccaag
     7201 cccaatgtgc gcctgcaccg gaacagcccg gaggcgctcc tggaccgggt cgtggacatg
     7261 atcgcgtcga gccagggggc gccgttcctc ctcaatttcg acgagcggtc gatggccggc
     7321 atgctgcggg aggcgaagga ggcgggcgtc acccacctga tccacgaggg gaacgtgcac
     7381 gactacgcct ccgtcggctg cctggagaac acgatggttg gcaacgaccg ttcaggcacg
     7441 gtggataaca acgtgaacct cgtcaaggcg gtcgagctgg ccctgaccgg ggggcgggac
     7501 ctcgtgccct tcgtcgatcc gatgaacggc aagacggagc gcatccgcca ggcggggccg
     7561 cggacgggcg acgcgacggc gtttgcgacc tgggacgcgt tctgggaggc ctactgtgcg
     7621 cagacccgct acatcctggc ccgctgcgcg gagacatacg aggcctcgga ggcgatccgg
     7681 gcccgcttct cgccgacccc ctacctgtcc tgcctcgtga agggctgcgc cgggcagggg
     7741 cgcgacgtga cccagggcgg cgccgagctc agcttcacca cgatggaggg ggtcaccttc
     7801 gccacaacgg tcgattcgct gctcgcgatc aagtatctcg tctacgacca gaaggcgtgc
     7861 acgatggcgg agctgatccg ggcgctcaag gacaactggg ccggccacga gatcctgcag
     7921 gccaaggcca agttccgggc cccgaagtac ggccgggacg acgacgaggc ggaccggatg
     7981 gccgcccggg tcatggagtt gtgggcggcg gaaacctgga agttccggac ccgctcgacg
     8041 ggccggcgct tccgccccgg catgctgagc tggaactact gggtcggcga cggcttcatc
     8101 gtggccgcca cggcggacgg gcgcccccgg gggcagttcc tctccaacgc catctgcccc
     8161 tccaacggcg ccgacatcca cggtcccacg gccaacgtga actccgtggg caaggccctg
     8221 ggcggcaagg cgccctccgg ccggggggac tgggaggcct acctgtgcaa cctgccgaac
     8281 ggggcgagcc acacgatgac cttcagcccc tccctgctgc gggaccccga acaccggcgg
     8341 aaattccggg ccttcctcaa gggttacatc gaaaacggcg gcacggcgct ccagatcaac
     8401 ctgatcgacg cggacatgct ccgcgaggcc cagaaacatc cccgggacta ccggcacctc
     8461 ctggtccggg tgacgggcta caacgcctac ttcacgagta tcggccgcga gctgcaggac
     8521 gagatcatcg cccgcgagag ccatgcccgc tactgaagga gacccatgac cgcgaccgcc
     8581 gccccggttg atgaatcgac cgaatccctg atcctgaaca tccagcgcat gtccaccgaa
     8641 gacggccccg gcatccggac gacggtcttc ttcaagggct gcaatcttca ctgcctgtgg
     8701 tgccacaacc cggagagcct ctccgcccgt ccccagatgc agtggatcga gacccgctgc
     8761 atcggctgca agacgtgtct cgcggcctgc ccggaggggg ccctgagcga cgacggcgcg
     8821 gggatccgca tcctgaggga ccgctgcacc gcctgcggcg cgtgcagccg ggcctgtccc
     8881 gccctggccc tggagcgtct cggggaaccc tggcggctgg acaggctgct ggacgaggtc
     8941 gagaaggacc gggcctattt cgaggcctcc ggcggcgggg tgacggtttc cggcggcgaa
     9001 ccggccctgc aggccccctt cgtcgcggcc ttactcgccg gctgccggca gcgcggcctg
     9061 cacacggcac tcgacacctg cggcttctgc gcgacggggg cgctcgaggg actcctgccg
     9121 gtcacggacc tcgtgctcta cgacttgaag gagatcgacc cggagcggca ccgccggttc
     9181 accggccagt ccaacgagcg gatcttcgcg aacctgctcc tcgtccggga ccggatgcgc
     9241 gcgcaggagc gaccggcgcg cctgtggctg aggacgccca tcatcccccg catgacggcg
     9301 cgggaggaca acatcgccgg tctcggccgc tttattgtgg aaaatctggc tgacgtggtc
     9361 gagcgctggg agctgtgcgc cttcaacaac ctctgccggg acaagtaccg ccggctcgac
     9421 atggactggg cctgccggga ggatgccctg atgacggccg gcgagatgga ggccctcgac
     9481 gacgcggccc ggcgggtggg cgtcgcagcg gcccgggtgg tctggacggg cgcggtccga
     9541 ccggacgaac ccgccggaga tcccgtatga gaagggccgg ttaaatccag gggggggatg
     9601 taatgacgga tctgcagacg cggttcttgc cggaagacag ggaggccttt gcctcggacg
     9661 tcaaggtggg cctactcgcc acggtgagcg gggaaggatg gccacacctg acgctgatca
     9721 cgacgctcga gacggcctct ccgacggaac tcacgtgggg ccagttctgc gaaggggcga
     9781 gcaagcggca cg
//
LOCUS       DAOOLJ010000066         9638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_8220_length_9638_cov_14.725034, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000066 DAOOLJ010000000
VERSION     DAOOLJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_9638_cov_14.725034"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLW16_08790"
     CDS             complement(<1..392)
                     /locus_tag="PLW16_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71043.1"
                     /translation="MNVDIMRKIDLFVGVPLCFVVTLLYRLNPFSRRRKPDPIRRVLF
                     IELSEMGSAILADPAMRKIRRRAGAELFFLIFRSTRPSLELLQTVPAENIFTLRGDRL
                     WTLAWDTVCFILWTRRQRIDTVIDLELFS"
     gene            707..1357
                     /locus_tag="PLW16_08795"
     CDS             707..1357
                     /locus_tag="PLW16_08795"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HPB71044.1"
                     /translation="MIDLHTHTIFSDGALIPSELARRAEVAGYRGLAFTDHGDAANID
                     FIIPRLVHVCRKLQSSYRIRLVPGIELTHVPPDDIAPLAGESRKLGARIVVVHGETLA
                     EPVAPGTNHAALLAGIDILAHPGLIAEEDVLLAARNGIALELSARRGHALANGHVAKL
                     ARRHGASLIVNTDAHAPGDLISAVQAARIAAGAGLDETEISALIAHAEALLKKIQL"
     gene            1441..2652
                     /locus_tag="PLW16_08800"
     CDS             1441..2652
                     /locus_tag="PLW16_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB71045.1"
                     /translation="MTYRGNPTCGGQGVYIYYLSHALKELGHHVEVISGPPYPVLDEG
                     VRLQKIPSLDLYNPDHLFKVRRLRDLTSPINQVEFLSMCSGGFPEPMTFGMRVRRHFQ
                     LHHPHYDVVHDNQCLAYGIGDLTRLGYPTVATIHHPITVDRDVDIQSARPWYRKIKVR
                     RWYSFIRMQQKVSRRLSHIITVSECSKRDISDAFAIPSQKFRVVPNGINMDYFYPLTH
                     VERQRDHLIVTNSADTPLKGLRYLLEAVASIRERRPIRLTVIGQPKPDGVIERLVRKL
                     RLKDDVHFTGRIPGEDFARYYAEATVAVIPSLYEGFGMPAGEAMACGVPVISTTGGAL
                     PEVVGDAGILVPPGNRKALEEAIVALLDDPERCHALGQAGLKRVREAFTWRHAAEKTV
                     GVYREAIHADR"
     gene            2639..3346
                     /locus_tag="PLW16_08805"
     CDS             2639..3346
                     /locus_tag="PLW16_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB71046.1"
                     /translation="MLTVDFSRLGIRPGMRILDVGCGTGRHLGAAFRTAGIDVVGVDL
                     NRDDLRRAAYTLHLICEEMADHGAWLITRADATNLPFDDARFDLVICSEVLEHIPDNR
                     KAVAELVRVLKPGCDLVVSVPRYWPERICWALSDDYWHEPGGHIRIYRKDELTRMLEA
                     AGTTCWGFGYRHALHVPYWWLKCAVGHKNESSRPVSLYRKFLEWDIIKRPLITRWLDA
                     LLNPVLSKSIVIYLKKG"
     gene            3352..4488
                     /locus_tag="PLW16_08810"
     CDS             3352..4488
                     /locus_tag="PLW16_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71047.1"
                     /translation="MELNVSRKLTLSPIDIDAMTAFIKRIQKMNGEIPWSLDGKTDPW
                     DHVESAMGLSMAGCRTEAERAYLWLVENQQEDGSWCAAYRDGRAEDRTRDTNMSTYIA
                     VGTFHHYLMTEDLNFLREIWPALKAGIDFAVQMQANSGEIYWAKNEAGVVDPMALLTG
                     CSSIYMSLKCGLAVARLLGKKKPAWERALAKLGHAIRQRPNLFNMIKSRFSMDWYYPV
                     LCGAITGAAAIKRMEKQWEKFVVPEWGVRCVSDRPWVTMAETSELVLALEAIGDYTQA
                     ELVFSWLQDKKFDDGSYWMGVTFPDRVIWPEEKTTWTAAAVLIAYDALMDLTPAAGLF
                     RHDFWERSGYLPADLLRDLPAAFPSPLSAGMYKVDARVSLSETR"
     gene            4492..5214
                     /locus_tag="PLW16_08815"
     CDS             4492..5214
                     /locus_tag="PLW16_08815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB71048.1"
                     /translation="MLRDHRPYILKRWDIRWQDWYAQHFLKPQFARMGKGGVFMRPWY
                     VEVFGGPISLGDYPHVIATPDKRVRLTVWSTIGGNGRISIGDYCLICPGVRISAGSEI
                     TIGNSCMMAQGAFITDSDWHDIYDRGRSVGQSAPVRIEDNAWIGDSAIVCKGVRIGKN
                     SIVGAGAVVAKDVPDNVIVAGNPASVVKEIDPARDMKTRAEWLADPAELAAQFDEIDR
                     SRMGANTLRGWLRALIFPRSGD"
     gene            5258..6850
                     /locus_tag="PLW16_08820"
     CDS             5258..6850
                     /locus_tag="PLW16_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB71049.1"
                     /translation="MKVAIIAPPYPLEEAPAPPLGVTYVAAAFEKAGADVRIFDYIVN
                     RYTPERLKEQLDRFRPDVVGATSVTLNFIAAADILRTAKRHNPRIVTLIGGPHVSFSE
                     RETLTAYPEIDVVVRGEGEKTIPELMPRLEDRRGWESVRGIAFRQGTDIVLTEPQELI
                     EDLNTLPLPARHLLPMSRYQALGYPVSIITGRGCPYACIFCQGRRMVGKKFRQRDASL
                     VVDEIEEILSYGIDRINVADDLFASNRDRVKAVCSEIQKRGLKFSWSAFARANTVDRE
                     MLRIMRETGCDSISYGVESGNQEMLDRIKKKITLDMVREAVHLGLEAGMVVHTSFIVG
                     LPGESPETLKETQAFADSLGAMYGYHYLAPFPGTTVREEVDQFDLEILTDDWNRYDAN
                     SAIVRTSKISPEEITRFVTDFENEINEAWEKQIRGYWEKTNPPEKDLHVEGFFRTQFV
                     FNLLSKDLIERYGRLPGPCSPEASLGELCARLVEATGGNEEIIRKAMQRFIEAGYIKQ
                     TREDDGYRWYWTHNNRVDFNSL"
     gene            complement(6941..7636)
                     /locus_tag="PLW16_08825"
     CDS             complement(6941..7636)
                     /locus_tag="PLW16_08825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB71050.1"
                     /translation="MVFPEIKALNIKGFLADAESAQLYKRASLAAKRGPCLEIGSYCG
                     LSTAYLGTGCREAGGVLFSIDHHGGSEEQQPGQAYFDPDLVNPSTGEIDTFPHFRRTL
                     RSLDLENTVIPIAARSELVARHWSTPLSLVFIDGGHTFQTALTDYVCWSPHLMPGGYL
                     LIHDIFPDPSQGGQAPFCIYRLALASGLYEDCGIVGTLAILQRPLGKDLTAAAISQWQ
                     ALQAAGTAKASDH"
     gene            7751..9301
                     /locus_tag="PLW16_08830"
     CDS             7751..9301
                     /locus_tag="PLW16_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPB71051.1"
                     /translation="MKTAELELERIRKLTQISIALSTQHDIDRLLEMIVEEARWLTRA
                     DGGTLYILSDDGRHLNFAIVQNESLRVRMGGTGGGITWPPIPMHLPDGRSNDQNVSAY
                     AALSGRTVNIDDVYAAAGFDFQGTRHFDQETGYRSRSMLVVPMYNHEQDIIGVLQLLN
                     AMDARTGGVVGFSDESEKITEAMASQAAVALTNRRLIQSLQHLLDAFITAIATTIDEK
                     SPYTGGHVRRVADLVVRLARKINEAKSGPFADIALDTDHMEELRMAAWLHDIGKITTP
                     EHIVDKATRLEAIVDRLELIRTRAEVIKRDYLIGCLRGRLAAAGLESEGGPASADDPF
                     LLAVEEDLDLLARINGGETRLDDALVSRLKAIGDRRGSTLEGGAFPLVTEDEMKSLRI
                     ARGTLTDEERFIVNQHALMTEKILQSLPFPKKFRNVPEYAGSHHEKLDGSGYPHGKRA
                     DALALQSRIIAVADVYEALTARDRPYKTMKTREESMALMERMAREGHLDEGLVVLLKE
                     AVASGDIR"
     gene            complement(9312..>9638)
                     /locus_tag="PLW16_08835"
     CDS             complement(9312..>9638)
                     /locus_tag="PLW16_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71052.1"
                     /translation="QFSCLDPRREGQLIALIRDTENDHLADVSWAIHLGCLRDDRTGD
                     FVSLDPDIQIFALSRNVQNHFLTDAYHESVWREVSACRFSIDWNCFQDRYSIYAKGRV
                     NPEAPA"
BASE COUNT         1981 a         2883 c         3009 g         1765 t
ORIGIN      
        1 gaaaaaagct cgaggtcgat gaccgtgtcg atccgttgac gccgcgtcca gaggatgaaa
       61 cacacggtat cccaggcgag cgtccaaagg cgatctccgc ggagcgtgaa gatattttcg
      121 gccggaaccg tttgaagaag ctccaggctt ggccgggtgg accggaaaat caggaaaaag
      181 agctctgccc cggcgcgcct ccggattttt ctcatggccg gatcggcgag gatcgcgctg
      241 cccatctcgg acagctcgat gaagagaaca cgccggatcg ggtccggttt gcggcgccgg
      301 gaaaagggat tgagccggta tagcaaggtg acgacgaagc agaggggaac gccgacgaaa
      361 agatcgattt tccgcatgat atccacgttc atgaccatcc tcgagtgggc cgcgtgcggc
      421 ccggaaggtg atttccggcg cactatagcg aaacgcgcgg tacgtgtcaa agattacgcc
      481 gcgtcttcgc gagtggcgga ctcgacgcgg ggatgcccgc gggcgaaccc ccaaccgacc
      541 cacggacaaa taattcttta gaaaaagccc ccagttcctt gttgacccaa aagattaaat
      601 ttgttaagtg catccattcc gacaacccag aatgctcatc tccgccgcgg acaaaggcgg
      661 atcatagcgt ggagtgagaa cggcggcatg cgaacggagc gcggcaatga ttgacctgca
      721 tacgcacacg atcttcagcg acggggcact catcccctct gaacttgcgc gccgggcgga
      781 ggtggccgga tatcgggggc tggcctttac ggatcacggt gacgcggcca acatcgattt
      841 catcattccc cgtctggtcc atgtctgccg taaacttcag tcctcctatc ggatcaggct
      901 cgtccccggc atcgaactga cccatgttcc gcccgacgac atcgcaccgc tggcggggga
      961 gtcgcgcaaa ctgggcgcgc ggattgttgt ggtccacgga gaaacgctcg cggagcccgt
     1021 tgcccccgga accaaccatg ccgcgctctt ggcggggata gacattctgg cccatcccgg
     1081 tctgattgcc gaggaagacg tgctcctggc ggcccgaaac gggatcgctc tggaactttc
     1141 cgcgcgacgg ggtcacgctc tcgcgaacgg gcacgtggcg aagctggcgc gcaggcacgg
     1201 cgcgagcctg atcgtcaata cggacgcgca cgcgccgggg gatctgattt cggctgtcca
     1261 ggccgctcgg atcgcagccg gcgccggctt ggacgagacg gagatatccg ccctgatcgc
     1321 ccatgcggag gcgctcctga agaaaataca actttgagga aggaaagaca gacgttgcca
     1381 aggcaagccg accgtcccgc aaccgggcat agcacacaca caccgttgaa gatctgcctc
     1441 ttgacgtacc gcggcaaccc aacctgcggg gggcaggggg tctatatcta ttatctgagc
     1501 catgccctca aggaactcgg acatcacgtc gaggtcatct ccgggccgcc gtatccggtc
     1561 ttggacgagg gggtgcgcct ccaaaagatt cccagcctcg acctgtacaa ccccgaccac
     1621 ctgttcaagg tccgacgcct gcgggactta acatcaccga tcaatcaggt cgaattcctg
     1681 agcatgtgtt ccggtggctt tccggagccg atgacgttcg gcatgcgcgt ccgtcgtcat
     1741 ttccagctgc accatcctca ctacgatgtc gtccatgaca accagtgcct cgcctacggc
     1801 atcggggatc tcacccgtct gggatacccg acggtggcga cgatccatca tcccatcacg
     1861 gtggatcggg acgtggacat ccagtcggcc cgaccctggt acaggaaaat caaggtccgg
     1921 cgctggtatt cctttatccg catgcagcag aaggtttccc ggcgcctgtc gcacatcatc
     1981 acagtctccg aatgctccaa gcgcgacatc agcgatgctt tcgcgattcc ctcgcagaaa
     2041 ttccgcgtag ttcccaacgg gatcaacatg gattatttct accccctcac gcatgtcgaa
     2101 cgccagcgcg accacctcat cgtgaccaac agcgccgata cgcccctgaa ggggctccgg
     2161 tatctcctgg aggcggttgc ctccatccgg gaaagacgcc ccatccgcct gaccgtgatc
     2221 ggacagccga agcccgatgg cgtgattgag cgcctcgtcc gtaaattgag gctaaaggat
     2281 gacgtccatt tcaccgggcg gattcccggc gaggatttcg cgcgctacta cgccgaggcc
     2341 accgttgcgg tcatcccctc gctctacgag ggtttcggca tgcccgcggg agaggcgatg
     2401 gcctgcgggg ttcctgtgat cagcaccacg ggtggggcgc tgccggaggt ggttggggat
     2461 gccgggattc tcgttccgcc aggcaacaga aaagcgctcg aggaggcgat cgtggccctg
     2521 ctcgatgacc ctgagcgctg tcacgcactg ggacaggccg ggctgaagcg ggtccgtgag
     2581 gcgttcacct ggcggcatgc cgccgagaag accgtgggcg tctatcggga agcgatccat
     2641 gctgaccgtt gatttcagcc ggctgggaat ccgccccgga atgcggatcc tggatgtggg
     2701 atgcggcacg ggtcgtcatc tcggcgcggc gttccggacg gcgggcatcg acgtggtcgg
     2761 ggtggatctc aacagggacg atctgcgccg ggcagcctac accctccatc tgatctgcga
     2821 ggagatggcc gatcacgggg catggctgat cacccgggcg gacgcgacga accttccgtt
     2881 cgacgacgcc cggttcgacc tggtgatctg ttcggaggtg ctggagcaca ttccggacaa
     2941 ccggaaggcc gttgccgagc tcgtccgcgt tctgaagccc ggatgtgacc tggtggtcag
     3001 tgtgccgcga tattggccgg agaggatctg ctgggccctc tcggatgact actggcacga
     3061 gccgggcgga catatccgaa tttacagaaa ggatgaactc acccggatgc tcgaggcggc
     3121 gggaaccacc tgctggggat tcggttaccg tcatgcgttg cacgttccct actggtggct
     3181 caaatgcgcg gtcggtcaca aaaacgaatc ctctcggccc gtgagcctct atagaaaatt
     3241 cctggaatgg gatattatca aacgtccttt gatcactcgc tggcttgacg cgctgctcaa
     3301 ccccgttctc tccaagagca tcgtgatcta tctcaaaaaa ggataataac tatggaattg
     3361 aatgtttccc gaaagttgac gctgagccca attgacattg acgccatgac cgccttcatc
     3421 aaacgcatac agaagatgaa cggcgagatc ccctggtcgc tggatggaaa aacggatccc
     3481 tgggaccacg tcgagagcgc catggggctg tcgatggccg gttgccggac ggaagcggag
     3541 cgggcctatc tctggctggt cgagaaccag caggaggacg ggagctggtg cgccgcgtac
     3601 cgggacggac gcgcggaaga ccggacgcgg gacaccaaca tgtccactta tatcgccgtc
     3661 gggacctttc atcattatct gatgacggag gacctcaact tccttcgcga aatctggccg
     3721 gccctcaagg cgggcatcga ttttgccgtg cagatgcagg ccaattccgg cgagatctac
     3781 tgggcgaaga acgaggccgg cgtcgtcgat ccgatggccc tactcacggg ctgcagctcg
     3841 atctacatga gtctcaaatg cggcctggcc gtcgcacgcc tcttggggaa aaagaagccc
     3901 gcctgggagc gggcgctcgc gaaactggga catgccatcc ggcagcggcc caacctcttc
     3961 aacatgatca agtcgcgctt ctccatggac tggtactacc cggtcctgtg cggcgcgatt
     4021 accggtgcgg ccgctataaa gcggatggaa aagcagtggg aaaagtttgt cgttccggaa
     4081 tggggggtcc gctgcgtttc ggatcgtccc tgggtcacga tggccgagac atcggaactc
     4141 gttctcgcct tggaggccat cggtgattac acccaggccg aactggtttt cagctggctg
     4201 caggacaaga aattcgacga cggatcctac tggatggggg tcacgttccc cgaccgggtc
     4261 atctggcccg aggaaaagac cacctggacg gctgcggccg tgttgatcgc ctatgacgcc
     4321 ctgatggatc tgacgccggc ggccggactc ttccggcatg atttctggga gcggagcggc
     4381 tatctgcccg cggatctgct tcgcgacctg ccggcggcat tcccctcgcc cctctccgcc
     4441 gggatgtaca aagtcgatgc gcgcgtttcc ctctccgaaa cgaggtaggc cgtgctgagg
     4501 gatcatcgcc cctacatcct gaagcgctgg gacatccgct ggcaggattg gtatgcgcaa
     4561 catttcctga agcctcaatt cgcacggatg gggaagggcg gtgtcttcat gaggccctgg
     4621 tacgtggaag tcttcggtgg accgatcagc ttgggagatt atccacacgt catcgcgacg
     4681 cccgacaagc gcgtccgcct gacggtctgg tccacgatcg ggggaaacgg acgtatcagc
     4741 atcggggact actgcctgat ctgtcccggc gtcaggatca gcgccggtag cgagatcacc
     4801 atcggcaaca gctgcatgat ggcccagggc gcctttatca cggattccga ctggcacgac
     4861 atctatgacc gaggccggtc cgtcggacaa tccgcgccgg tccggatcga ggacaacgcc
     4921 tggatcgggg acagcgccat cgtctgcaag ggcgtccgta tcgggaagaa cagcatcgtg
     4981 ggcgctggcg ccgtggtcgc gaaggacgtg ccggataacg tcatcgtcgc cggtaatccc
     5041 gccagcgtcg tgaaggagat cgatccggcg cgggacatga aaacgcgcgc ggaatggctc
     5101 gcggatccgg cggagctcgc cgcgcagttc gatgaaatcg accggtcccg gatgggagca
     5161 aataccctgc ggggctggct tcgtgccctc attttccccc gatccgggga ttagcggcag
     5221 gacccaaaat tcgaacactc aaggaaaacg gacccccatg aaagtcgcca tcattgcgcc
     5281 accctatccc ctggaagaag cccccgcacc ccccttgggc gtcacctatg tggccgccgc
     5341 cttcgaaaaa gcgggagcgg acgtccgcat cttcgattat atcgtcaatc gctatacgcc
     5401 cgaaaggctg aaagagcagc tcgaccgatt cagaccggac gtcgtcggcg caacctccgt
     5461 gacgctcaat tttatcgccg ccgccgacat cctgcgcacg gcaaagcgcc acaatccccg
     5521 catcgtaact ctgataggcg gcccgcacgt gtctttctcg gagcgggaaa cgctgacggc
     5581 gtatccggag atcgatgtcg tggtgcgggg cgagggggaa aaaaccatcc ccgaactcat
     5641 gccccggctg gaggaccgcc gtggctggga aagcgttcgc ggcatcgcgt tccgccaggg
     5701 gacggacatc gttctgacgg aaccccagga gctgatcgag gatctcaata cgctgccgct
     5761 tccggcgcgg catctgttgc ccatgtcgcg ttatcaggcc ctgggatatc cggtgagcat
     5821 catcacgggg cggggctgcc cctatgcctg cattttctgc cagggccgtc gcatggtggg
     5881 caagaagttc cgccagcgcg atgcctcgct cgtcgtcgat gagatcgaag aaatcctatc
     5941 ctacggcatc gaccggatca acgtggccga cgatcttttc gcctcgaacc gggatcgcgt
     6001 caaggccgtc tgtagcgaga ttcagaagcg gggcctgaag ttcagctgga gcgcctttgc
     6061 ccgggccaat accgtcgatc gcgaaatgct caggatcatg cgcgagacgg gatgcgacag
     6121 catcagctac ggcgtcgaat cgggcaatca ggaaatgctc gatcggatca agaaaaagat
     6181 caccctggac atggttcgcg aggccgtcca tctcggcctc gaagccggca tggtcgtcca
     6241 tacgtccttc atcgtggggc tgccgggcga atctccggag accctcaagg aaacgcaggc
     6301 cttcgccgac agcctgggcg ccatgtacgg ctatcactac ctggcgccat tcccgggaac
     6361 gaccgttcgt gaagaggtcg atcaattcga cctggagatc ctcaccgacg actggaaccg
     6421 atacgacgcc aacagcgcca ttgtccggac atccaagatt tcgcccgagg agataacgcg
     6481 attcgtcacg gatttcgaga acgagatcaa cgaggcctgg gaaaagcaga tccggggtta
     6541 ctgggaaaag acgaaccccc cggagaagga tctgcacgtg gagggtttct tccggactca
     6601 gtttgtcttc aatctgcttt cgaaggacct gatcgagcgt tacggtcggc tgcccggtcc
     6661 ctgttcccca gaggcgtcct tgggggagct ctgcgcccgg ctggttgagg cgacgggcgg
     6721 gaatgaggag atcatccgga aggcgatgca gcgatttatt gaagcgggat acatcaagca
     6781 gaccagagaa gacgacggct atcgctggta ctggacacat aacaaccgcg ttgattttaa
     6841 ttccttgtaa gaaacgggac tggtggctcc gggaattcca tgaggcaagg gtccgagagg
     6901 cgcagcggtt tctcgtcgaa aggccgctgg taaagaagcg ttagtgatcg gaggctttgg
     6961 ccgtgccggc cgcctgcagc gcctgccact ggctgatggc ggcggctgtc agatccttcc
     7021 cgaggggccg ctgaagaatc gccagggtcc caactattcc gcaatcctca tacaggccgg
     7081 aggcgagggc cagtcggtag atgcagaagg gagcctgtcc cccctggctc ggatcgggga
     7141 agatgtcgtg gatcaggaga tatccgccgg gcatgagatg aggagaccag caaacgtagt
     7201 cggtcagggc ggtttggaag gtatgcccac cgtcgatgaa gacgagcgac agcggcgtgg
     7261 accagtggcg cgccacgagt tccgagcggg cggcgatcgg gatcacggtg ttctcgagat
     7321 ccaggctccg gagggtccgg cgaaaatgcg gaaacgtgtc gatttctccc gtgctcgggt
     7381 tgacgaggtc cggatcaaaa taagcctggc caggctgttg ctcttcggat ccgccgtgat
     7441 gatcgatgga gaagagcacc ccccccgctt cccggcatcc cgttcccaga taggccgtgg
     7501 aaagaccgca atagctcccg atttcgagac agggcccccg ctttgcggct agactggccc
     7561 gcttgtacag ctgagcagac tctgcgtcgg ccaggaagcc cttgatgttc aacgccttga
     7621 tttccggaaa gaccatgacc cccttctccc tgacggcgaa cggttgctct cgttccgtgg
     7681 ggggtgtcat accgtgatga aggacaaaat gcaagaaaat gtgcgtcaga tcacgttttg
     7741 agggggctcg atgaagacgg ccgaactgga attggaacgg atcagaaagc tgacgcagat
     7801 cagcattgcc ctgtcgacac agcacgatat cgaccgcctg ctggagatga tcgtcgagga
     7861 ggcccgctgg ctgacccggg cggacggggg caccctgtac atcctctccg atgatggtcg
     7921 gcatctgaat tttgccatcg tacagaacga gtcgctccgg gttcgtatgg gcggaacggg
     7981 aggcgggatc acgtggccgc cgattcccat gcatctgccg gacggccgat ccaatgatca
     8041 gaacgtctcc gcctacgccg ccctgtcggg aagaaccgtc aatatcgacg atgtgtacgc
     8101 cgcggcaggt ttcgatttcc agggaacacg ccatttcgac caggagaccg ggtaccgctc
     8161 ccgctccatg ctggtcgtgc ccatgtacaa tcacgagcag gacatcatcg gtgtcctgca
     8221 gcttttgaat gccatggacg cacggacggg cggggtcgtc ggtttcagcg acgaaagcga
     8281 aaagatcacg gaggcgatgg cctcacaggc cgcggtcgcg ttgaccaacc ggcggctgat
     8341 tcaaagcctg cagcatctcc tcgacgcctt catcaccgct atcgcgacca ccatcgacga
     8401 gaagtcgccc tacacaggcg gtcacgttcg gcgggtggcc gacctcgtcg tgcgtctggc
     8461 ccgaaagatc aacgaggcga agtcgggacc cttcgccgac atcgctttag acaccgatca
     8521 catggaggag ctccggatgg cggcctggct gcacgacatc ggcaagatca ccacgcccga
     8581 gcacatcgtc gacaaggcga ctcggctcga agcgatcgtc gaccgcctcg aactgatccg
     8641 gacccgggcg gaggtcatca agcgggatta tctcatcggt tgtctgcgcg gaagacttgc
     8701 cgccgccggc ctggagtccg agggcggccc cgcttctgcg gatgacccgt tcctgctcgc
     8761 cgtggaagag gacctcgacc tgctcgcccg gatcaacggc ggggaaaccc ggctggacga
     8821 tgccctggtg tcccgcctga aggcgatcgg cgacaggcgg ggaagcacac tggagggggg
     8881 ggcctttccc ctcgtcacgg aggatgaaat gaaaagcctc cggattgccc gggggacgct
     8941 gacggacgag gagcggttca tcgtcaacca gcatgccttg atgacggaaa agatcctgca
     9001 atcgctgccc tttcccaaga agttcaggaa tgttcccgaa tacgcgggct ctcaccacga
     9061 gaagctcgac gggtcgggct atccccacgg caaacgggcg gacgcccttg cgcttcagag
     9121 ccgcatcatt gccgtggcag acgtctatga agcgctgacg gccagggacc ggccttacaa
     9181 gaccatgaaa acccgggagg agtcgatggc gctgatggag cggatggccc gggagggaca
     9241 tctcgacgaa ggcctcgtcg tcttgttgaa ggaggccgtg gcctccggag acatccgctg
     9301 acggcgttgc ctcaagccgg ggcttcgggg ttgacccggc cttttgcgta tatggaatac
     9361 cggtcctgaa aacaattcca gtcaatgctg aagcggcagg ccgacacttc ccgccagacc
     9421 gattcatggt aggcgtccgt taggaaatgg ttctgcacgt tcctggagag cgcgaagatc
     9481 tgaatgtccg gatccaagga aacgaagtct cccgtgcggt catcccgcag gcaacccagg
     9541 tgtatcgccc aagaaacgtc ggccagatga tcgttctcgg tatcgcggat gagcgcgatc
     9601 aattgtccct cccggcgcgg atccagacag gaaaactg
//
LOCUS       DAOOLJ010000067         9580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_8294_length_9580_cov_20.850919, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000067 DAOOLJ010000000
VERSION     DAOOLJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8294_length_9580_cov_20.850919"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..627
                     /locus_tag="PLW16_08840"
     CDS             55..627
                     /locus_tag="PLW16_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71053.1"
                     /translation="MKTSLKLIELVRDKGEDIAGNWARDVRKNAKTPFYHDLSDEKIL
                     PQAIHFYEHLLKIVNLDDPFAGARDYFSGYAERLYRDGVPLNEAVYALVLMRRHIWLY
                     AEFQVTFITAIEQNQAVESLLRTTLIFDYIMYFVTETYETLLKRQAAPKRTTRKKGDY
                     VIPWSSWASLWGEGRDKKEEAFHNLNDPAR"
     gene            633..2018
                     /gene="atpD"
                     /locus_tag="PLW16_08845"
     CDS             633..2018
                     /gene="atpD"
                     /locus_tag="PLW16_08845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB71054.1"
                     /translation="MERYENQGTIVSVRGSVVDAFFPKQLPAMRQVLRAGENQRIQIE
                     VFTQLDAHTIRGVALTPTQGLARGSVIVDTGQPFTVPIGKRLLGRVFNVFGDTIDKLE
                     PVSGGEWRSIHNEPVALIEQSTVAEIFETGIKAIDVLAPLERGGKAGLFGGAGVGKTV
                     LITEMIHNMVSHHEGVSIFCGIGERCREGEELYREMKDAGVLGNTVMVFGQMNEPPGA
                     RFRVGHAALTMAEYFRDDQKQDVLFLIDNIFRFIQAGSEVSGLMGQLPSRLGYQPTLA
                     TELAELEERISNTKTGAITSIQAVYVPADDFTDPSASHTFGHLSASIVLSRKRTSEGL
                     YPAVDLLQSNSKMLMPNIAGERHYRIAQDIRKTLSVYEDLKDIIAMLGIAELSREDQR
                     TVFRARRLERFFTQPFFVTEQFTGTDGKMVALADSLDGCERILNDEFADYPESALYMI
                     GDISEAKIHEA"
     gene            2008..2397
                     /locus_tag="PLW16_08850"
     CDS             2008..2397
                     /locus_tag="PLW16_08850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPB71055.1"
                     /translation="MKLRILLPAEVMLEEEVTKVVAEAENGSFCLLPHHTDFAATLAP
                     GIFTYTTAAGDEALLAMDTGALIKQGANVLVSTRNAIRAEDLDRIKQVVEKQYQVLDE
                     REKMARAASARLEADLIRRFMELNQTR"
     gene            2457..2825
                     /locus_tag="PLW16_08855"
     CDS             2457..2825
                     /locus_tag="PLW16_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPB71056.1"
                     /translation="MENEKTSQERRRQIAATFTKKIAQRQAMRIRGQKQKDRIVWFGL
                     GMIGVVGWSVAIPTLIGVALGLWIDQNLPSRFSWTLMLLIAGVTAGCMNAWYWVKRGG
                     YNIEHLNGETDEEETHEKRR"
     gene            2809..3111
                     /locus_tag="PLW16_08860"
     CDS             2809..3111
                     /locus_tag="PLW16_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPB71057.1"
                     /translation="MRSAADFLPAFAAGLALGAVFFGGLWWTVRRLVAAPRPSRLLWQ
                     SFALRFGIALAGFYAVANGQGGPLAVAVLGFLVMRELIARRFFIRADSSGGMPWKS"
     gene            3099..3842
                     /locus_tag="PLW16_08865"
     CDS             3099..3842
                     /locus_tag="PLW16_08865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPB71058.1"
                     /translation="MEVMTLQMISPDQIVLWKWAFITINATLLFTWLVMGILVCASFL
                     ITRRLSAGTRIPRWQNLLEVIVGAMREQIREVTEEDTDEYMPFIGTIFLFILTSNLLF
                     IIPGYVTPTASLTTTAALAVCVFLAVPIYGIRKQGIVNYLKEYFQPIFIFFPFHVMGE
                     FSRTLALAVRLFGNMMSHEKVIAILLAITPLLFPVAMHILGLLIGAIQAYIFAILAMV
                     YIASATGAHHEKQPSAAVPSEAVKLASAK"
     gene            3873..4148
                     /locus_tag="PLW16_08870"
     CDS             3873..4148
                     /locus_tag="PLW16_08870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HPB71059.1"
                     /translation="MSIIGMASVIASAICMGIGSIGTGLGEGRAVQQALMAIAQQPDE
                     KGALTRTLFVGLAMIESIAIYCFVISMILIFANPFWNQVIPKVVAGG"
     gene            4153..4923
                     /locus_tag="PLW16_08875"
     CDS             4153..4923
                     /locus_tag="PLW16_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71060.1"
                     /translation="MHIDGFVFFAQIVNFLILIYLLKRFLYGRIIQAMDNREAKIAAR
                     IAEAETMKVDAAAAAENYAQKMKGLDARFEALLNEARLEAEERRKELLAKAREEVDGI
                     QKNWQEALKRQQEAFLQELRRLTGQQLWTIVGRVLADLADADLEQRIVKVFLHRLTTL
                     DEQRRLEIKASLRGEGELYIDSAFAIAPEAQEQIRSVLKGHFTNGVRIFYRVRPEVVS
                     GIEMRAHGHKIAWSFQEYMETMQENLSQALQREAQGTA"
     gene            4936..6528
                     /locus_tag="PLW16_08880"
     CDS             4936..6528
                     /locus_tag="PLW16_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HPB71061.1"
                     /translation="MEGNAKSRESTSRGDDGVLKSFLASTFSTLDEVLLSQQPDLTLH
                     EVGVVKFVSRGIVRVDGLPNIRAEELVRFPGNLMGLVFNIDPDEVGVILLDPSEHIGA
                     GAEVHRTGRVLDVPVGDAMLGRIVDALGRPLDNKGDIAAAKRLPVEREAPAVMDRLPV
                     TVPLQTGIKVIDALIPIGRGQRELILGDRQTGKTAIILDTIINQKGKDVICIYCAIGK
                     QSSDTARVVADLEAHGVMDFCTIVVATGEDPPGLQFIAPYAATSMGEYFMSQGRDVLV
                     VYDDLTRHARAYRELSLLLRRPPGREAFPGDIFYIHSRMLERSTHLRAELGGGSLTAL
                     PITETEAQDMSSYIPTNLISITDGQIYLSPPLFQKGILPAIDVGKSVSRVGGKTQLPA
                     YRAVAGDLRLSYSQFEELESFSRFGTRLDESTRRTLERGWRVREILKQNQYVPLPVAE
                     QIAALLSVTGGALDEVPLERVGEAEAAIRQAVREQLVDLCARIQAGEKLGLSDKEAML
                     RVARDVVAKKFREESRADNGSA"
     gene            6506..7438
                     /locus_tag="PLW16_08885"
     CDS             6506..7438
                     /locus_tag="PLW16_08885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HPB71062.1"
                     /translation="MQTTEALKRTIKSAGDLLSVVKTMKALAAVSIRQYQKAVESLAD
                     YNRTVEMGLQILLKSQPDLTLLSRPQPVRRLGVIVFGSDQGLCGQLNEQIATHALSEM
                     DALKVPRSERTILSAGMRVTDYIEDAGQRVEETLTTPGSTSGITPMVQEIIMILESWR
                     FEKHIDHFVLYYNKYLSGASYRPITVPLLPVDPTWLRDIAKKKWPSRSLPIFTLDPDV
                     LLSTLVREYLFVSLYRAFAESLASENASRLASMQNAEKNIEERLQELHVHYHRQRQMT
                     ITEELLDIVAGFEALQEQARLKARSAHAERRVSS"
     gene            7435..>8235
                     /locus_tag="PLW16_08890"
     CDS             7435..>8235
                     /locus_tag="PLW16_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPB71063.1"
                     /translation="MTSSDKRFCAICAWRGTCQKRFSVPVDGLLNVYCPDYTRDIQIK
                     DTRDLDRALLDYQISQWQAEARKKVGPTITFSRETGSGGSEIARIVATRLKMDLIGGQ
                     IIQKVAESAKMSTKVIETLDEKGVTFMESIVNSMFASRHIWPDEYLRHLTMVIGAAGQ
                     HGNAVLMGRGANFILKPERTFRLRIIAPLEKRVQYLMERGKLSRSDAEKFIVNKDADR
                     KKFIKKNFKADVSDPAHYDLVVNTAYVSVEGAVAAIIDAFQARTFVFGQ"
     assembly_gap    8236..8335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8336..8564
                     /locus_tag="PLW16_08895"
     CDS             <8336..8564
                     /locus_tag="PLW16_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71064.1"
                     /translation="KEGPGSPGKMERLERELLLALAEEVVLQLRNRLMEAETQHPLES
                     AIRIADFQERLWCIEDLTDKIRTQVRKETNL"
     gene            complement(8572..>9580)
                     /gene="glk"
                     /locus_tag="PLW16_08900"
     CDS             complement(8572..>9580)
                     /gene="glk"
                     /locus_tag="PLW16_08900"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPB71065.1"
                     /translation="RRSRKGGDMLLAGDIGGTKTHLGVFSPYGDPREPLHAATLPSGR
                     YPDPETMIREFLAEAQLPVTAVCLGVAGPVREGAANITNLPWQLSVGRLCDVLGTPSV
                     RIVNDVEAMAWAIPALGPADLEVLSPGEPAERGTLALIAPGTGLGEAFLTWNGFQYRV
                     HPSEGGHVDFAPAGEQQAALLTGLQAQLDHVGYETVCSGQGLRRLYEFLRDGGDGHEP
                     AWLTEALTEADDPNPVIVAGALERGDGLCRRTLDLFAAILAAEAGNLALKVMADGGVY
                     LGGGIPRRILPVLRAPAFLAAFRRKGRMAGTVARIPVTVIVYERAALLGAVLCGLASF
                     P"
BASE COUNT         1800 a         3170 c         2983 g         1527 t
ORIGIN      
        1 aaggctgaag tcaaaagccc ccggcacatg cgaaagcgga aaggagcagg gtccatgaag
       61 acatcgctga aactgatcga gctggttcgg gacaaggggg aagacatcgc cggaaactgg
      121 gccagggatg tccggaagaa cgccaaaaca cccttctatc acgatctatc cgacgaaaag
      181 atcctgccgc aggccatcca tttctacgaa cacctgctta agatcgtcaa cctcgacgac
      241 cctttcgccg gcgcccggga ttacttctcc ggctatgcgg agcggctcta ccgggacggg
      301 gtccccctga acgaggccgt ctatgcgctg gtcctgatgc gccgtcacat ctggctctac
      361 gccgagtttc aggtcacctt catcacggcg atcgaacaga atcaggccgt ggaaagcctg
      421 ctccgcacga cgctcatctt cgattacatc atgtacttcg tcaccgaaac ctacgagacc
      481 ctcctgaagc gtcaagccgc gccgaagcgg acgacccgga aaaagggcga ttacgtcatc
      541 ccgtggtcca gctgggccag cctctggggg gaggggcgcg acaagaagga ggaggcattc
      601 cataatctca atgaccctgc gaggtgaacg acgtggaaag gtatgaaaat cagggaacga
      661 tcgtttcggt ccggggaagc gtcgtggacg ccttcttccc caaacagctc ccggccatgc
      721 gccaggtttt gcgggccgga gaaaaccagc ggatccagat cgaggtcttc acccagttgg
      781 acgcgcacac catccggggg gtggccctca cccccaccca ggggctggcg cggggatcgg
      841 tcattgtcga cacgggccag ccgtttaccg tgcccatcgg caagcgcctg ctgggccgcg
      901 tcttcaatgt cttcggggat accatcgaca agctggagcc ggtcagcggc ggcgagtggc
      961 gctccatcca caacgagccc gtcgccctga tcgaacagtc caccgtggcg gagatcttcg
     1021 agacggggat caaggccatc gacgtcctgg cgccgctgga gcggggcggc aaggctggcc
     1081 tgttcggcgg cgcgggcgtg ggcaagacgg tgctcatcac cgagatgatc cacaacatgg
     1141 tgagccatca cgagggcgtg agcatcttct gcgggatcgg cgagcgctgc cgggaaggcg
     1201 aggagctcta ccgcgagatg aaggacgccg gcgtcctggg caacacggtc atggtcttcg
     1261 ggcagatgaa cgagccgccc ggcgcccggt tccgggtggg acacgccgcc ctcacgatgg
     1321 cggagtactt ccgggacgac cagaagcagg acgtcctgtt tctgatcgac aacatcttcc
     1381 gcttcatcca ggcgggctcc gaggtctccg gcctcatggg gcagctgccc tcccgtctgg
     1441 gctaccagcc gaccctggcc acggagcttg cggagctcga ggagcggatc tcgaacacga
     1501 agacgggggc catcacctcc atccaggccg tctacgtccc ggcggacgat ttcacggacc
     1561 cctccgcctc ccacaccttc ggacacctgt ccgcctccat cgtcctgtcg cgcaagcgga
     1621 caagcgaggg cctctacccg gcggtcgacc ttctccagtc caactccaag atgctcatgc
     1681 ccaacatcgc cggcgaacgg cactaccgca tcgcccagga catccgcaag accctctccg
     1741 tctacgagga cctgaaggac atcatcgcca tgctgggcat cgcggagctc tcccgagagg
     1801 accagcgcac cgtcttccgg gcccgccggc tggaacggtt cttcacgcag cccttcttcg
     1861 tcacggagca gttcaccggc accgacggca agatggtggc cctggccgac tccctggacg
     1921 gctgcgagcg gatcctgaac gacgagttcg ccgactaccc cgagagcgcc ctgtacatga
     1981 tcggcgacat cagcgaggcg aagatccatg aagcttagaa tcctgctccc cgcggaggtc
     2041 atgctcgagg aagaggtcac caaggtcgtg gccgaggccg aaaacgggtc gttctgcctc
     2101 ctgccccacc acaccgactt cgcggccacc ctggcgccgg gcatcttcac gtacacgacc
     2161 gcggcggggg acgaggcgct cctggccatg gacacgggcg ccctgatcaa gcagggggcg
     2221 aacgtgctcg tctccacccg caacgccatc cgggcggagg atctggacag gatcaagcag
     2281 gtggtggaaa aacagtatca ggtgctcgac gaacgggaaa agatggcgcg cgccgcctct
     2341 gcccggctgg aggccgacct gatccggcgg ttcatggagc tgaaccagac gcggtagcgg
     2401 cgtgaagctc ccggcggtac accccctcac cctcgatcaa ggagacgagg tccggcatgg
     2461 aaaacgaaaa gacatcccag gagcggcgcc ggcagatcgc cgcaaccttc accaagaaga
     2521 tcgcccagcg gcaggccatg cgcatccggg gccagaagca gaaggaccgc atcgtctggt
     2581 tcggattggg gatgatcggc gtcgtcggct ggtcggtggc gattcccacc ctgatcggcg
     2641 tggcgctcgg cctgtggatc gaccagaacc tcccgagccg cttctcctgg accctgatgc
     2701 tgctgatcgc cggcgtgacg gccggttgca tgaacgcctg gtactgggtg aagcgggggg
     2761 gctacaacat cgaacacttg aacggcgaaa cggacgaaga ggagacccat gagaagcgcc
     2821 gctgatttcc tgccggcctt cgcggccggc ctggccctgg gcgccgtctt tttcggcggg
     2881 ctctggtgga ccgtccgccg cctcgtcgcc gcgccgaggc cgtcccggct cctctggcag
     2941 agcttcgccc tccgcttcgg catcgcgctg gcggggttct acgcggtggc gaacggacag
     3001 ggcgggccgc tggcggttgc ggtcctggga tttctcgtta tgcgcgaact catcgcgcgg
     3061 cgctttttca tccgcgcgga ttcctcagga ggcatgccat ggaagtcatg acgctgcaga
     3121 tgatcagtcc cgaccagatc gtcctctgga agtgggcctt catcaccatc aacgcgaccc
     3181 tgctcttcac ctggctggtc atggggatcc tggtctgcgc ctcctttctg atcacgcggc
     3241 ggctgtccgc gggcacccgg atcccacgct ggcagaacct gctggaggtc atcgtcgggg
     3301 cgatgcgcga acagatccgg gaggtgaccg aagaggacac ggacgaatac atgccgttca
     3361 tcgggacgat cttcctgttc atcctcacct cgaacctcct gttcatcatc cccggctacg
     3421 tgacgcccac ggcatccctg accaccacgg cggccctggc ggtctgcgtt ttcctggccg
     3481 tgcccatcta cggcatccgc aagcagggaa tcgtgaatta cctcaaggag tacttccagc
     3541 ccatcttcat ctttttcccc tttcacgtca tgggcgagtt ttcccgaacc ctggcgctgg
     3601 ccgtccggct cttcggcaac atgatgagcc atgagaaggt catcgcgatc ctcctggcca
     3661 tcacccccct gctctttccg gtcgccatgc acatcctggg gcttctgatc ggcgccatcc
     3721 aggcctacat cttcgcgatc ctcgcgatgg tctacatcgc ctccgccaca ggggcgcacc
     3781 acgaaaagca accaagcgcc gcggttccgt cggaagcggt caaactggct tcggccaaat
     3841 aaacaaagaa aggaaggaag actatggaca gcatgagcat cataggaatg gcttcggtga
     3901 tcgcttccgc gatctgcatg gggatcgggt ccatcggcac gggactcggg gaggggaggg
     3961 ccgtccagca ggccctgatg gccatcgccc agcagccgga tgaaaagggc gccctgaccc
     4021 ggaccctgtt cgtgggtctg gcgatgatcg agtccatcgc catctactgc ttcgtgattt
     4081 ccatgatcct gatcttcgcc aatccgttct ggaatcaggt cattccaaag gtcgtcgcgg
     4141 gaggataggt ccatgcatat cgacgggttc gtattttttg cccagatcgt caacttcctg
     4201 atcctgatct atctgctgaa gcgttttctc tacggccgga tcatccaggc catggacaac
     4261 cgggaggcga agatcgccgc ccggattgcc gaagccgaga cgatgaaggt ggatgcggcg
     4321 gcggcggcgg agaattacgc ccagaagatg aagggtctcg atgcacggtt cgaggcgttg
     4381 ctcaacgagg cgcggctgga agcggaggaa cggcgcaagg agctgctcgc caaggcccgc
     4441 gaagaggtgg acgggatcca gaagaactgg caggaggccc tcaagcgcca gcaggaggcg
     4501 ttcctccagg agctgcgccg cctgacgggc cagcagctct ggaccatcgt cggccgcgtg
     4561 ctggccgatc tggccgacgc cgacctggag cagcggatcg tcaaggtctt tctccaccgc
     4621 ctcaccaccc tggacgagca gcggcggctg gagatcaagg cctcgctccg gggcgagggg
     4681 gagctctata tcgacagcgc cttcgccatc gctccggagg cccaggagca gatccggtcc
     4741 gttctcaaag gccatttcac caacggcgtc cgcatcttct accgggtccg gccggaggtc
     4801 gtctccggaa tcgagatgcg ggcgcacggg cacaagatcg cgtggagttt ccaggagtac
     4861 atggaaacga tgcaggaaaa tctctcccag gccctgcagc gggaggccca gggaacagcc
     4921 taaaggaaga ggatcatgga agggaacgca aagtcgagag aaagcacgtc acggggcgat
     4981 gacggagtct tgaagtcctt cctcgccagc accttttcca ctctggacga ggtcctgctg
     5041 agtcagcagc ccgacctgac gctccacgaa gtcggggtcg tgaagttcgt cagccggggg
     5101 atcgtccgcg tcgacggcct gcccaacatc cgggcggagg aactcgtgcg cttcccggga
     5161 aacctgatgg gactcgtgtt caacatcgac ccggacgagg tcggcgtgat cctcctcgac
     5221 ccgagcgaac acatcggcgc cggcgccgag gtccaccgca ccggccgggt cctggacgtt
     5281 cccgtggggg acgcgatgct gggacgcatc gtggacgccc tgggccgccc cctggacaac
     5341 aagggggaca tcgccgccgc gaaacggctc cccgtcgagc gggaggcccc ggcggtcatg
     5401 gaccggctcc ccgtcaccgt ccccctccag acggggatca aggtgatcga cgccctcatc
     5461 cccatcggcc gcggccagcg cgagctgatc ctgggcgacc gtcagacggg caagaccgcc
     5521 atcatcctgg acacgatcat caaccagaag ggcaaggacg tcatctgcat ctactgcgcc
     5581 atcggcaagc agagctccga cacggcccgg gtcgtcgccg atctcgaggc ccacggggtg
     5641 atggacttct gcacgatcgt cgtcgccacg ggggaggacc cgccgggatt gcaattcatc
     5701 gccccctacg cggccacctc gatgggggag tacttcatgt cccagggccg ggacgtcctc
     5761 gtcgtgtacg acgacctgac ccgccatgcc cgcgcctacc gggagctctc cctcctcctg
     5821 cgccgcccgc ccggccgcga ggcctttccc ggcgacatct tctacatcca ctcccggatg
     5881 ctcgaacggt ccacccatct gcgggcggag ctgggcggcg ggtcgctcac cgccctgccc
     5941 atcacggaga ccgaggccca ggacatgtcg tcgtacatcc cgaccaacct gatctccatc
     6001 accgacggcc agatctacct ctccccgccg ctcttccaga agggcatcct gccggccatc
     6061 gacgtgggca agtccgtctc ccgtgtcggc ggcaagaccc agctgcccgc ctaccgggcc
     6121 gtcgccggcg acctgcggct gtcctactcg cagttcgagg agctggaatc cttctcccgg
     6181 ttcggcaccc gcctcgacga gagcacccgc cggacgctgg aacggggctg gcgggtccgg
     6241 gagatcctga aacagaacca gtacgtcccc ctgcccgtcg cggagcagat cgccgcgctg
     6301 ctgtccgtga ccggcggcgc cctggacgag gtgcccctcg agcgggtcgg cgaggcggaa
     6361 gcggccatcc gtcaggcggt gcgggagcag ctggtcgacc tgtgcgcccg catccaagcg
     6421 ggggaaaaac tcggcctgtc ggacaaggag gccatgctgc gggtggcccg ggacgtcgtg
     6481 gccaagaaat tcagggagga atcccgtgca gacaacggaa gcgcttaagc gaaccatcaa
     6541 gagcgccggg gacctcctgt cggtcgtcaa gacgatgaag gccctggccg ccgtcagtat
     6601 ccgccagtat cagaaggccg tcgaatcgct ggcggactac aaccggaccg tggaaatggg
     6661 tctgcagatc ctgctcaaga gccagccgga cctcaccctg ctgtcccggc cccagccggt
     6721 tcggcgcctg ggggtcatcg tcttcggctc cgatcagggc ctgtgcggtc agttgaacga
     6781 gcagatcgcc acgcacgccc tttcggagat ggacgccctc aaggtgccgc ggtcggagcg
     6841 gacgatcctg tccgcgggga tgcgcgtcac cgattacatc gaggacgcgg gccagcgggt
     6901 ggaggaaacc ctgaccacgc ccggctcgac ctccgggatc acgcccatgg ttcaggaaat
     6961 catcatgatc ctggaaagct ggcgcttcga gaaacacatc gaccatttcg tcctgtatta
     7021 caacaagtac ctgtccggcg cgtcctaccg gccgatcacc gtcccgctcc tgcccgtgga
     7081 ccccacctgg ctcagggaca tcgcgaaaaa gaaatggccg tcccggagcc tgcccatctt
     7141 caccctggat cccgacgtcc tgctgtccac gctggtgcgg gaatacctct tcgtttccct
     7201 ctaccgggcc ttcgccgaat ccctggccag cgaaaacgcc agccgtctgg cctccatgca
     7261 gaatgccgag aagaatatcg aagagcgtct ccaggagctc catgtccact accaccggca
     7321 gcggcagatg accatcacgg aggagctcct cgacatcgtc gccggcttcg aggcgttgca
     7381 ggagcaggcc cgattgaagg cgcgcagcgc gcatgcagaa aggagggtgt catcatgacg
     7441 tcgagcgaca agaggttctg tgcgatctgt gcctggcggg gaacctgtca aaaaaggttc
     7501 agcgtccccg tcgacggtct gctgaacgtc tactgccccg attatacgcg ggacatccag
     7561 atcaaggata cccgggatct ggaccgggcg ctactggact atcagatcag ccagtggcag
     7621 gccgaggccc ggaaaaaggt cggcccgacg atcacctttt cccgggagac gggcagcggc
     7681 ggcagcgaga tcgcccggat cgtggccacc cggctcaaga tggacctgat cggcggccag
     7741 atcatccaga aggtggcgga aagcgccaag atgagcacga aggtcatcga aaccctggac
     7801 gagaagggcg tcacgttcat ggagagcatc gtcaactcga tgttcgccag ccgccacatc
     7861 tggcccgacg aatacctccg ccacctgacc atggtcatcg gtgccgccgg tcagcacgga
     7921 aatgccgtcc tcatgggccg gggcgccaac ttcatcctca agccggagcg gaccttccgc
     7981 ctgcgcatca tcgcgcccct ggagaagcgg gtccagtacc tgatggagcg cggcaaactt
     8041 tcccggagcg atgcggaaaa gttcatcgtg aacaaggatg cggaccggaa gaaattcatc
     8101 aagaaaaact tcaaggcgga cgtctccgac ccggcgcact acgatctggt cgtcaacacc
     8161 gcctatgtca gcgtggaggg cgccgtggcc gccatcatcg acgccttcca ggcccggacc
     8221 ttcgtcttcg gccagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaag
     8341 aggggccggg gagcccggga aagatggaac ggctggaacg ggagctcctg cttgcgctcg
     8401 ccgaggaggt cgtgctgcag ctgcgcaaca ggctcatgga agccgagacg cagcaccccc
     8461 tcgaatcggc catccgcatc gccgacttcc aggaacggct ctggtgcatc gaagacctga
     8521 ccgacaaaat ccgcacgcag gtccgcaagg aaacgaacct ttgagcccgg cctacggaaa
     8581 ggacgccagg ccgcagagca cggcgcccag cagggcggcg cgctcgtaga cgatcacggt
     8641 taccgggatc cgcgccaccg tcccggccat ccggcccttc cggcgaaagg ccgccaggaa
     8701 ggccggcgcc ctcagaaccg gcaggatgcg ccggggaatc cctccgccga ggtacacgcc
     8761 gccatcggcc atgaccttca gggccaggtt gcccgcctcc gccgccagga tggcggcgaa
     8821 gaggtccagc gtccggcggc agaggccgtc ccccctctcc agggcccccg caacgatgac
     8881 cgggttcgga tcgtccgcct ccgtcagggc ctcggtcagc caggccggct cgtggccgtc
     8941 gccgccgtcc cggagaaatt cgtagaggcg gcgcaacccc tgtccggagc agaccgtttc
     9001 atagccgacg tgatcgagct gcgcctgcag cccggtcagc agggccgcct gctgttctcc
     9061 cgccggcgcg aaatccacat gccctccctc ggaggggtgg acccggtact gaaagccgtt
     9121 ccaggtcaga aaggcctccc ccagtccggt ccccggggcg atcagggcca gggtcccccg
     9181 ttccgccggc tcccccgggc tcagcacctc cagatccgcc ggccccagcg cgggaatggc
     9241 ccaggccatc gcctcgacgt cgttgacgat cctgacggac ggcgtcccca gaacgtcgca
     9301 gaggcggccg acgctcagct gccaggggag gttcgtgatg ttggcggcgc cctcccgcac
     9361 cggtcccgcg acgccgagac agaccgccgt cacgggcagc tgggcctccg ccaggaattc
     9421 ccggatcatc gtctccggat cgggataccg cccgctgggc agggtcgccg cgtggagcgg
     9481 ttcccgcgga tcgccgtaag gggaaaaaac gcccagatgg gtcttcgttc cgccgatgtc
     9541 gcccgccagc agcatgtcgc ctccttttcg ggatctccgg
//
LOCUS       DAOOLJ010000068         9342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_8604_length_9342_cov_11.470335, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000068 DAOOLJ010000000
VERSION     DAOOLJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8604_length_9342_cov_11.470335"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLW16_08905"
     CDS             complement(<1..898)
                     /locus_tag="PLW16_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB71066.1"
                     /translation="MDKPGIPEQVEDILIVGGGPAGYTAGLYAARARMKTLMIEGEST
                     VSQITVTDLIENYPGLPEGVNGFDLRERFRAQAESFGMRRATGDVTAIAPVSWGGTSG
                     WRVTAGETTYDTLAVIVAAGANWRRLGVPGEEEFIGRGVSYCATCDGPFYRNRDVVVV
                     GGGNTAVQEALYLTRFASRVILVHRRDRLRAAGVLAERASAHPKIAFAWNSVVQAVEG
                     KDFVEGIRIQNVRNPAEVRVIPAEGVFVFIGLTPNTDLLVGIADRDANGYVRVDPDMR
                     TSAPGIFACGDCIQKLLRQVITA"
     gene            complement(1054..2358)
                     /locus_tag="PLW16_08910"
     CDS             complement(1054..2358)
                     /locus_tag="PLW16_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71067.1"
                     /translation="MSRSGWIIGVLLLCLLPSPAAAEESYVFDLSEIEKKPYHFSGYL
                     EAKPVVYGLDDRAAFYQLRFFNDSHRRTVWEANGRVRLEGGYEKGPARIFARLNTDLQ
                     YNDKIHASGKTTFYEAYASVKPLPSLKIDAGKKTMKWGKGYAWNPVAFLDRPKNPDDP
                     EQAMEGYIVATADVIRSFDGPLKTVSFTPAFLPVCNHVNTDFGNADKLNFSARLYFLL
                     FDTDVDFMFMTGGSRPDRYGVDFSRNVGSNFEIHGEYAWLKSSRKIVLDPSGTARRVE
                     TDAHRFLIGLRYLASFELTTIVEFYHAGDGFTSDEMESFFHLIHRGYGAYRATGNPAG
                     LGRAAQLAESGYARPNPMRNYLYVRLIQKDPFDILYFSPSLTCMINTDDLSVSVMPEL
                     LYTGIANMELRLRASVLAGARDTDFGEKQNDYRIELRAGIYF"
     gene            complement(2359..3093)
                     /locus_tag="PLW16_08915"
     CDS             complement(2359..3093)
                     /locus_tag="PLW16_08915"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPB71068.1"
                     /translation="MLKIVHFVLAVILVFPVHALALDGAKLLREMDRNLSPESYESYR
                     KLINIEPNGRKKEFTLFTVKKGLDKVASVFLAPASEKGRSTLRLGENMWLNIPNVGKP
                     IRITSLQSVVGGVFNNADILQLDYAVEYDVTGVREEKGELLLHLKAKTKNVAYDRLKI
                     RLDRNRTLPTSIECLTEANMVIKTLYFKNVRDFGGGIVRPEVIETDSPLYKGYKSVMI
                     MAKIKKRTFKDEVFTLTFMPNLASLR"
     gene            complement(3105..3491)
                     /locus_tag="PLW16_08920"
     CDS             complement(3105..3491)
                     /locus_tag="PLW16_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB71069.1"
                     /translation="MKKRLLSGFLWVLMLIVSGTNLWADNTRIAVVSQGNTPDAVVSP
                     VAARGLYFLIFDVQGTFIEALKNPYRDAGRNASEQVVADLSNRGVTTLIAGNFGNKMT
                     AAMTARRIRYVEFQGTAADAVRKAVP"
     gene            complement(3502..4743)
                     /locus_tag="PLW16_08925"
     CDS             complement(3502..4743)
                     /locus_tag="PLW16_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB71070.1"
                     /translation="MRNLFKIAIRNLIRYQRRTLLTASLITIGVVFVLVFVSISGSFK
                     SLMIGQITDSMMGHLQLHRKGYVASIDNLPLNLNMKPETYRKIEEMLASDQDVAAFSP
                     RLKFGGMFSNFIETTNIRLNGIDPEAEFRTVPLLSARVIEGRPTLGRGEILVPALLAK
                     GMKLRPGDPVVVVATNADGSVNGMQFIVSGILESATGPGGRDGYVHIGDAAEVLRMTE
                     VEVSEIAVRIEDFSGLPEVMTRLRQNIRDDGPQQKGQAALEVHSWEDLSPFFNIARMI
                     DVMTLFIRIMLIAIVLISIMNVMVMAVYERIREIGTIAAIGTPPGKIRSMFILEGFCM
                     GVFGAAAGVGIGAAVIALVNLIGIRFNFGQQKGLLLKAVLDPLDLMVVSAIVVAVAVL
                     ASLQPAVKASRMEPIDALRHV"
     gene            complement(4736..5440)
                     /locus_tag="PLW16_08930"
     CDS             complement(4736..5440)
                     /locus_tag="PLW16_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB71071.1"
                     /translation="MALITASGVSKEYRTEEVTVHALRELHFEIEPASFVSFVGPSGS
                     GKTTLLNLLGCLDKPTAGTLSVAGTEVAALNRKESAAFRGKHLGFIFQDFNMISVLTV
                     YENIEYPLIMVQNVAPRERRERIARMLAAVGMADQGGKYPHQISGGQKQRVAIARALV
                     TNPALVLADEPTANLDSQTAYAVIGLMKKMRDEMGTTFIFSTHDTKIVGEAEVLFRLV
                     DGRLAEKEVKGGTTHA"
     gene            complement(5520..6242)
                     /gene="prxU"
                     /locus_tag="PLW16_08935"
     CDS             complement(5520..6242)
                     /gene="prxU"
                     /locus_tag="PLW16_08935"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084511687.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5988..5990),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPB71072.1"
                     /translation="MAKEFKAGCARPTGGPVGEKLEKPQGTPQQTPHKEVKKMITVGQ
                     KAPDFTAPAYHKGKFVSVQLSEFLGKWVVLCFYPGDFTFVUATEISAVAETYPEFKKL
                     GVEVLSMSVDSVFVHKMWNDHELSKMVKGGVPFPMLSDGGGKVGSVYGVYLEDAGVEA
                     RGRFLIDPDGIIQGFEVLTPPVGRNVRETIRQIQAFQHVRKSKGSEATPSGWKPGKPS
                     LKPGPGLVGKVWEVWNTKMAFD"
     gene            complement(6466..7947)
                     /locus_tag="PLW16_08940"
     CDS             complement(6466..7947)
                     /locus_tag="PLW16_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB71073.1"
                     /translation="MIGGSAAGPKAAAKARRIDNDAEVIILQKEADLSMASRGYPYYV
                     GGFFDDRNQLLCTPAGVVRNPMFYLNAKDIEARVNTEVTAIDRKHKTLSFKNLADGEA
                     GTLPYDKLVIATGAVPRMPPVPGADLDGISTLQSMKDADFLRNVRDEGKIKKAVVIGG
                     GLIGIETCEALQLAGIEITVIELLPQLLTFLDWELAKLVENHVKSKGANVITDNGIAA
                     FLGENGKLTAVKLENGTELPCELAVVAIGVRPNVKLAKEAGLKIGELGGIDVNEYMQT
                     SDPDIYAVGDCVESVNRITGKKVHAPYGDLANLQGRVAGENAASENYVTFPGTIQTGI
                     CKVFDYAAGSTGLSETAARKLGYTDIVTVVNASPDKPGFMEGKLLVTKLTADAKSGRI
                     LGAQCIGPGNVAKQIAQWAMAIQGKLTVEDLINADLPYAPPFSLAIDHFIATAHLLQN
                     KMKGRLKGISCHQVYLKILAGEKPFLIDTRGPEEFEEIRLGIG"
     gene            complement(8094..8780)
                     /locus_tag="PLW16_08945"
     CDS             complement(8094..8780)
                     /locus_tag="PLW16_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB71074.1"
                     /translation="MQTEFVAWMEGIPLFQGVSREGLRSLAARARLRTFRSGEAILGE
                     TDPVRAFFVVIDGRVKLYKSSSEGKEQTLCLLGVGEPFGLCTAFATPAFPANVSALED
                     SAVVVIPGPELEAAARQEPVLLLNVIRILSRRLKESMALVESLSLYEIPQRLSAFLLQ
                     SPNGRRKGAPGLVRLATTHRELAKILGATPEALSRALKRMTADGILRVEGRDISILDR
                     AALEAVAAGE"
BASE COUNT         1798 a         2853 c         2773 g         1918 t
ORIGIN      
        1 aggccgtgat cacctgccgg agcagttttt gaatgcaatc gccgcaggcg aagattcccg
       61 gggcggaggt ccgcatgtcc ggatcgaccc gaacataccc gttcgcatcg cggtccgcga
      121 tgccgaccag gaggtccgta ttcggcgtaa gcccgatgaa gacgaagacc ccttccgccg
      181 gaatgacgcg gacctccgcg gggttccgga cgttctggat gcggatcccc tccacgaaat
      241 ccttcccctc gaccgcctgg acgacgctgt tccaggcgaa ggcgatcttc ggatgggcag
      301 aggcacgctc ggccaagacg cccgccgccc gcaggcgatc ccgccggtgg acgagaatca
      361 cccggctcgc gaagcgggtc agatagagcg cctcctgaac cgccgtgttc ccgccgccga
      421 ccaccaccac gtcgcgattg cggtagaagg ggccgtcgca ggtcgcgcag taagaaacgc
      481 cccggccgat gaattcctcc tcccccggga cgcccaagcg ccgccagttc gcccccgccg
      541 cgacgatcac ggccagggtg tcgtaggtcg tttcaccggc cgtcacccgc cagccggacg
      601 tcccgcccca ggagacgggc gcgatggcgg tcacgtctcc ggttgcccgc ctcatcccga
      661 aggactcggc ctgggcgcgg aaacgctccc gcaaatcgaa cccgttgaca ccctccggga
      721 ggcccggata gttctcgatc agatccgtca ccgtgatctg gctgacggtg gattccccct
      781 cgatcatcag ggtcttcatc ctggcccggg cggcgtagag ccccgccgtg tatcccgccg
      841 gcccgccgcc gacgatgagg atgtcttcaa cctgttctgg tattcctggc ttgtccatgt
      901 cgatgcactc ctgagaggcg ccctcacggc gccgttcggt tcggtcgagg cagacccgcc
      961 cccgctgaga tacggcgggc agtataggaa aaggggcgag ggatgtcaac ggaattcgtg
     1021 ggaaatttcc cgaagactta cgcagtttcg atcctaaaag taaatgcccg cccgcagttc
     1081 gatccgatag tcgttttgct tttctccgaa gtcggtgtcc cgcgcccccg caaggacaga
     1141 tgcccgcaac cggagttcca tgttggcgat gccggtatag agcagctccg gcatgacgct
     1201 gacgctgaga tcgtcggtat tgatcatgca ggtcagggaa ggggagaaat agaggatgtc
     1261 gaaaggatcc ttctggatga ggcgaacgta caggtaattg cgcatggggt tgggacgggc
     1321 atagccggat tcggcgagct gcgccgctcg tcccagtccc gccggatttc ctgtcgcccg
     1381 ataggcacca tatccccggt gaatcaggtg gaagaagctc tccatttcgt cggaggtgaa
     1441 accatcgccg gcatgataaa attcgacgat cgtcgtgagt tcgaaggagg cgagataacg
     1501 caggccgatg agaaaccggt gggcgtccgt ttcgacccga cgtgccgtcc cggatgggtc
     1561 caaaacaatt ttgcggctgc ttttcagcca ggcgtattcc ccgtggattt cgaaattgct
     1621 gccgacgttg cgggaaaaat cgacgccgta gcgatccggt ctgctgccgc cggtcatgaa
     1681 catgaaatcc acatccgtgt cgaagaggag gaagtacaga cgggccgaaa agtttagctt
     1741 gtcggcgttg ccgaaatccg tgttgacgtg attgcagacg ggcaagaacg ccggcgtgaa
     1801 ggaaacggtt ttgagcggtc catcgaaact gcggatcacg tccgccgtcg cgacgatgta
     1861 gccttccatc gcctgttccg gatcgtccgg attcttgggc cggtcgagaa aggccacggg
     1921 attccaggca tagcccttgc cccatttcat cgtcttcttg cccgcgtcga tcttcagtga
     1981 cgggaggggt ttgaccgacg cgtaagcttc gtagaaggtc gtctttcccg atgcgtggat
     2041 tttgtcgttg tactgcaagt cggtattgag tcgggcgaag atccgggcag gacctttttc
     2101 gtagcccccc tccaggcgta cacggccgtt ggcctcccac acggtccgac gatgggagtc
     2161 gttaaagaag cgcagctgat agaacgcggc ccggtcgtcc aggccgtaga cgacgggctt
     2221 ggcttcgaga tagcccgaga aatggtacgg ctttttttcg atttcggaga ggtcgaagac
     2281 gtagctttcc tcggccgccg ccggagacgg gaggagacag agcagcagaa ccccgatgat
     2341 ccatccggat cgactcatct aacgcaggga cgccaggttc ggcatgaagg tcagcgtgaa
     2401 gacctcgtcc ttgaaggtgc gcttcttgat tttggccatg atcatgacgg acttgtagcc
     2461 cttgtaaagc gggctgtccg tttcaatcac ctccggacgg acgatgcccc cgccgaaatc
     2521 ccggacattt ttgaagtaaa gggttttaat gaccatgttc gcttcggtga ggcattcgat
     2581 gcttgtgggc agcgttctgt tcctgtcgag cctgattttc aatcggtcgt aggccacgtt
     2641 tttcgttttg gccttgaggt gaagcagcag ttcgcctttc tcttcccgga ccccggtcac
     2701 gtcgtattcc acagcatagt ccagttggag aatgtcggcg ttgttgaaca cgccacccac
     2761 caccgattgc aggctggtga tgcggatggg tttgccgaca ttgggaatgt tcagccacat
     2821 gttttctccc aggcgaagcg tgctccggcc tttttcactg gcgggcgcca gaaagacgga
     2881 ggccaccttg tccaaaccct tcttgaccgt gaacagtgtg aattccttct tccggccgtt
     2941 cggttcgata ttgatgagct ttcggtagga ctcataggat tcgggggaaa ggttgcggtc
     3001 catctcccgc agcaatttcg ctccatccaa ggccagggca tgcaccggaa agacaaggat
     3061 cacggccaga acgaaatgaa cgatcttaag catcgctggt cctcctatgg aaccgctttc
     3121 cggacggcat cggcggcggt cccctgaaat tcaacatagc gaatgcgcct ggccgtcatg
     3181 gcggccgtca ttttatttcc gaaattgccg gcgatgaggg tcgtaacgcc tcgattcgac
     3241 agatcggcaa caacctgttc gctggcgttc cggccggcat cccgataggg atttttcaag
     3301 gcttcgatga aggtcccctg gacgtcaaag atgagaaaat aaagaccccg cgccgcaacg
     3361 ggactgacca cggcatccgg cgtatttccc tgggagacca ccgctattct ggtattgtcc
     3421 gcccagaggt ttgtgcctga cacgatcaac atcaggaccc acagaaagcc cgacagaagc
     3481 ctttttttca taaccttccc cctatacatg tctgagcgcg tcgatgggtt ccatccggga
     3541 ggccttaacg gccggctgga gacttgccag cacggcgacg gccacgacga tcgccgacac
     3601 gaccatcaga tccagcgggt cgagcacggc cttgagaagc aggccttttt gctggccgaa
     3661 gttaaagcgg atgccgatca ggttgacaag ggcgatgacc gcggccccga tcccgacacc
     3721 ggctgccgct ccgaacaccc ccatgcagaa tccctccaga atgaacatgg aacggatttt
     3781 ccccggcggg gtgccgatgg cggcaatcgt gccgatttcg cggatgcgct cgtagacggc
     3841 catcaccatg acgttcatga tgctgatgag cacgatggcg atgagcatga tccggatgaa
     3901 gagggtcatg acgtcgatca tacgggcgat gttgaagaaa ggagataaat cctcccagga
     3961 atggacctcg agcgccgcct gtcccttctg ctgaggaccg tcgtctcgga tgttctgcct
     4021 cagccgcgtc atgacctcgg gcaggccact gaaatcctcg atgcggacgg cgatttcact
     4081 gacctccacc tcggtcatgc gcagcacttc ggccgcatcg ccgatgtgaa cgtagccgtc
     4141 gcgcccgccg ggtccggtcg cgctctccag aatgccggag acgataaact gcatgccgtt
     4201 gaccgagccg tcggcgttgg tggccaccac cacgaccgga tcgccgggac gcagcttcat
     4261 gcccttcgcc agcagcgctg gaaccaggat ttcgcctctg cccagcgtcg gccggccctc
     4321 gataacccgg gcggacagga gcggaacggt tcgaaactcg gcttccgggt cgatgccgtt
     4381 taagcggatg ttggtcgtct cgatgaaatt gctgaacata cccccaaact tgagccgcgg
     4441 agaaaaggcc gccacgtcct gatcggatgc gagcatttcc tcgattttcc gataggtctc
     4501 gggcttcatg ttcaggttga gcggcaggtt gtcgatggag gcgacgtaac ccttccggtg
     4561 cagttggagg tgtcccatca tcgaatcggt gatctggccg atcatcagag atttgaagga
     4621 cccgctgatg gaaacgaaca caaggacgaa aaccacaccg atcgtgatga gggaggccgt
     4681 caacagggtc cggcgctgat agcggatcag gttgcggatg gcgatcttaa acagattacg
     4741 catgcgtcgt cccccccttc acctcttttt cagccaagcg gccgtccacc agacggaaaa
     4801 gaacttccgc ctcgcccaca atcttcgtgt catgcgtgga aaagataaag gtggtcccca
     4861 tttcatcgcg catttttttc atcagcccga ttacggcgta ggcggtctgg gaatccaggt
     4921 tggctgtggg ctcatcggcc agtaccagcg ccgggttagt cacgagcgcc cgggcaattg
     4981 ccacccgctg tttctggccg cctgagatct ggtgggggta tttgccgccc tggtccgcca
     5041 tgccgaccgc cgcgagcatg cgggcgattc gttcccgccg ctcccgcggg gcgacattct
     5101 gaaccatgat cagggggtac tcgatgtttt catagacggt gagaaccgaa atcatgttga
     5161 aatcctggaa gataaacccc aggtgctttc cccggaacgc cgcgctttcc tttcggttca
     5221 gcgcggcgac ctccgttccc gccaccgaca gggtccccgc cgtgggctta tccaggcagc
     5281 cgagcagatt caggagcgtc gtcttgccgc tgccggaggg ccccacgaag gacacgaacg
     5341 aggccggttc gatttcaaag tggagttccc ggagggcgtg aacggtcacc tcttccgtcc
     5401 ggtattcctt ggatacgccg cttgccgtaa tcaaggccat cgcgaagccc ctttccggcg
     5461 gtcgcgcctg tcggcgggcg gccttcctcg ggccgcccgc cgcgacggtt cccgcttcct
     5521 caatcgaacg ccatcttggt gttccagacc tcccagacct tgccgaccag gcccggtccg
     5581 ggcttcagcg agggtttccc cggcttccaa cccgagggcg tcgcctccga gcccttgctc
     5641 ttgcgcacgt gctggaaggc ctggatctgc cggatcgtct cccgcacgtt gcgacccacc
     5701 ggcggcgtga ggacctcgaa gccctggatg atcccgtcgg gatcgatcag gaaacgcccc
     5761 cgggcctcca cgcccgcatc ctccagataa acgccgtaaa cggagccgac cttgccgccg
     5821 ccgtcggaca gcatcgggaa gggaaccccg cccttgacca tcttggacaa ctcgtggtca
     5881 ttccacatct tgtggacgaa aacgctgtcc acgctcatgg acagcacttc cacgcccagc
     5941 ttcttgaatt cagggtaggt ttcggcgacc gccgaaatct ccgtcgctca gacgaaggtg
     6001 aagtccccgg ggtagaagca caaaaccacc catttgccga gaaattccga tagctgaacg
     6061 gaaacgaact tgcccttgtg gtacgcgggc gccgtgaagt ccggcgcctt ctggccgacg
     6121 gtaatcatct tcttgacctc cttgtgcggt gtctgctgag gggttccctg gggcttttcc
     6181 aatttctccc cgaccgggcc gccggtgggc ctggcgcacc cggccttgaa ttcctttgcc
     6241 atcgggtcga cctcctttct caatacgggg ttatttgtcg cgcggatatg gccacgccat
     6301 gatcccgcct tccatgacct tgacattctt ccagccgttg gcccccaggg cgagtgccgc
     6361 ttcgtagccg cgcagcgaaa ttttgcagta gcagatgatt tcgcggtttt tatcttgcgg
     6421 cagttccttg aggcgtttgc ggatggcccc cagcggaatc agtgtctagc cgatgcccag
     6481 gcgtatttct tcaaactctt ccggtccgcg cgtatcaatc agaaaaggct tttcgccggc
     6541 cagaatcttc agatacacct ggtggcaact gatccccttg agccgtcctt tcattttgtt
     6601 ttgtagcaga tgcgccgtgg cgatgaaatg atcgatggcc agagaaaacg gcggcgcgta
     6661 gggcaaatcg gcgttgatca agtcctcaac ggtcagtttg ccctggatag ccatcgccca
     6721 ctgggcgatc tgtttggcga cattgcccgg accgatacac tgcgcgccca gaatccggcc
     6781 gcttttggca tcggcggtca gtttcgtgac caggagcttt ccctccataa atcccggctt
     6841 atcgggactg gcgttcacaa cggtgacgat gtcggtatat cccaactttc tggccgccgt
     6901 ctcggacagc cccgtggagc cggccgcgta atcgaacacc ttgcagatac cggtttgaat
     6961 ggttccggga aaggtcacgt agttttcgga tgcggcgttc tcgcccgcca cgcggccctg
     7021 aagattggcc aggtccccgt aaggtgcgtg cacttttttg ccggtgatgc gattgacgct
     7081 ctccacgcag tcgccgaccg cataaatatc ggggtcggag gtctgcatgt attcattgac
     7141 gtcgatcccg cccagttcgc cgattttcaa acctgcctct ttggccagtt ttacattggg
     7201 acgcacgccg atggccacca ccgcgagttc gcaggggagt tccgttccat tttccaattt
     7261 gacggcggtg agcttcccgt tttcacccag aaaggcggca atgccgttgt cggtgatcac
     7321 gttggcccct ttgcttttga catggttttc aaccagtttg gccagttccc aatccagaaa
     7381 agtcagaagt tggggcagaa gttcgatgac ggtgatttca atgcccgcca gttgcagcgc
     7441 ctcacaggtc tcgatgccga tcagcccgcc gccgatcacg acggcttttt tgatcttgcc
     7501 ttcgtcgcgc acgttgcgca gaaaatccgc gtctttcatc gattgcagcg tagagatgcc
     7561 gtccagatcc gcacccggca ccggcggcat gcggggcacc gcgccggtcg caatcaccag
     7621 tttgtcgtaa ggcagggtcc cggcctcacc gtcggccagg ttcttaaacg atagggtttt
     7681 atgcttccgg tcgatggccg tgacttccgt attcacccgc gcctcgatgt ccttggcgtt
     7741 caaatagaac atggggtttc gcaccacacc cgccggcgtg cacaaaagct ggttgcggtc
     7801 gtcgaagaaa ccgcccacgt aatacgggta gccgcgggaa gccatcgaaa gatccgcttc
     7861 tttctgcaaa atgatgacct cggcatcgtt gtcgatccgg cgggctttgg ccgccgcttt
     7921 gggaccggcg gccgatccgc cgatcacgat gatccgtctt gggttcgtca tggtcctctc
     7981 ctacgggatg aaatctaaag aaacgtcagg aggccggaaa cgcccgttgt cgtctcttcg
     8041 cagtccccct aacagtttac gaccgcgacg gcattgatgt aaatcaagat ccgtcattcc
     8101 ccggcggcga cggcctccag cgccgcccgg tccaggatgg agatgtcccg accctcgacc
     8161 cggaggattc cgtccgcggt cattctcttc aatgcccgcg agagggcttc gggcgtcgcc
     8221 ccgagaatct tggccagctc gcggtgggtc gtggcgaggc ggaccaggcc gggggcgccc
     8281 ttccttcgcc cgttcggact ttggagtaga aaggcggaca accgctgggg aatctcgtac
     8341 agggacaggg actcgacaag ggccatggat tccttcagac ggcgggagag aattcggatc
     8401 acgttgagca gcaggacggg ttcctgccgc gccgcggcct cgagttccgg tcccgggatg
     8461 acgacgacgg cgctgtcctc cagggcggac acgttggccg ggaaggccgg agtggcgaag
     8521 gccgtgcaga gcccgaaggg ttcccccacc ccgaggaggc agagggtctg ttccttgccc
     8581 tcggacgagc ttttgtagag cttgacgcgg ccgtcgatga cgacgaaaaa ggcgcggacg
     8641 ggatccgtct cgccgagaat cgcctcgccg gaccggaagg ttcgcagccg ggcccgcgcg
     8701 gccagggacc gcaggccctc ccgggaaacg ccctgaaaga gcgggatccc ctccatccac
     8761 gcaacgaact ccgtctgcat ctatcccccc gttgatccag atcaatgacg gacacggcga
     8821 aacggtcgta atgtttaact ttgctgttat catagcataa ggacggcggg gaggccaacc
     8881 cattttccgc ttcccgctgg acgtgaaaca tcggatcgac acgattggag gacgtcatga
     8941 aagaactgtg gatcgtcttg ggcatcgttg ccgcgtggat ttttctgcag gcctggctgc
     9001 tgccccggat gggcgtctcg acctgaatga gacctgcctg tccggtcgag gacagccgaa
     9061 agaaagcgcc tgccccggcg ccccgtgcga tcgccgacgc cccggagccc cagggaccgg
     9121 aacaaacccc ctgagggcag agggcaaaca acgattagag cgagcgctcg tttcttcttg
     9181 acagggtcgc gtgcctggcc tatggttcgg caacattcca tttctacatc ccgatgttgc
     9241 ccagaggagg aaaagcgcca tgcccgagaa ccccttgcac gccctgatga atccgaaatc
     9301 gatcgccatc gccggcgcga gcaacaatgc catgaagatg gg
//
LOCUS       DAOOLJ010000069         9279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_8677_length_9279_cov_19.318842, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000069 DAOOLJ010000000
VERSION     DAOOLJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8677_length_9279_cov_19.318842"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLW16_08950"
     CDS             <1..243
                     /locus_tag="PLW16_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71075.1"
                     /translation="AYQVDEIMAFTLRTFGQGTKVAVEISLMAADAGLVRTDEDVIAV
                     GGTVSGCDTALLLQPANSAHVLDLKIREVIAKPSVF"
     gene            complement(295..483)
                     /locus_tag="PLW16_08955"
     CDS             complement(295..483)
                     /locus_tag="PLW16_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71076.1"
                     /translation="MATLIPEGEDLKQAITWISAAIEDNPNQSLPALVEKAVFQFDLS
                     PVDAEFLVGFFANRNRTA"
     gene            complement(578..1246)
                     /locus_tag="PLW16_08960"
     CDS             complement(578..1246)
                     /locus_tag="PLW16_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPB71077.1"
                     /translation="MRRILVAAPHPDDEWIGCGGSLLKHIRRGNPVTVVYMTSGEAGG
                     LGRTREALAAVREAEAREAAAFAGITDLIFLGNPDGYLAYDRSNLVGLVEIVRRIRPD
                     ILYIPHAQETHPDHAATHRLCVEACRRAGGPWFSESPGEPWAVGTILCYEVWTPLTAV
                     PCVEDISAFMDLKLAALRRHRSQLDGIPYDEAAAGLNRYRGITTGKGRFCECFAVQSL
                     EAPS"
     gene            complement(1338..1553)
                     /locus_tag="PLW16_08965"
     CDS             complement(1338..1553)
                     /locus_tag="PLW16_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71078.1"
                     /translation="MKARSGKEHRWCDRHHYFIDLDACEARADQSPRCRRCIHQWNQL
                     CLPLPEFLNPPVALPRGGARTGKNGFR"
     gene            complement(1664..1987)
                     /locus_tag="PLW16_08970"
     CDS             complement(1664..1987)
                     /locus_tag="PLW16_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71079.1"
                     /translation="MAKARKGDMYSCEVCGLIVVVDEVCGCATADLVCCKAPMGRGKA
                     AAAKAKKKALAQPAAKAPALKAPAAKPAAKAKTKVKPAKKAAPKAAVKKAAPKKPAAK
                     AKKRA"
     gene            complement(2096..3163)
                     /locus_tag="PLW16_08975"
     CDS             complement(2096..3163)
                     /locus_tag="PLW16_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB71080.1"
                     /translation="MTSSTACPDMNVLMLAPTAFPRCTGNAVTVDRIARLLSRRNISC
                     EILDLSRTSPETLRKRVRSAAPDLLHAFHAVKGGTTGLRLQEQLGVPLITTLTGTDIN
                     LDFADPDKRRVMREVLNRSAAVTVFQETLLPRLNRLGIPRDKIRVIHQSVDFPERRDY
                     DYRAGLGLSGDTPVILMLGGLRQVKQMDYAVAALNKVRRHHRTFQLLIAGPLLEPAAT
                     EALHKALRIHPWIRYLGEVPRNQVPSLLEQSNLVLNTSRSESESNAILEALSFGRIVI
                     ARDIPGNAHLGAGAAGLLFRNREELERHLLTILEQPERWAAVGKNARRRIAAFHRPDR
                     EMKAYAALYRDVSRPSRHARR"
     gene            complement(3138..3992)
                     /locus_tag="PLW16_08980"
     CDS             complement(3138..3992)
                     /locus_tag="PLW16_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB71081.1"
                     /translation="MEPRILAQDIGDASLSYLLYEGDGPAIVMLHATGFLPWLWHPVA
                     RKLAPSFRVIAPYFCDHRDTDPNRGGLSWLEIARDLTEFCRRLDLDHPLLVGHSMGAT
                     VLAIAHAECGLAARGMLLVEPIFLPRDYYRTLITVEDHPLASKAIRRTNHWPDENAAL
                     AYLRSRALFEKWDEEMLRLYIRYGMMDDDAGGLTLTCAPEREAALFMGGMQYDPWPVL
                     PRVTCPVLMLEGEASENRAFIDLKEALSHIPHTPEYRLIPDAGHLIPMEQPRTVTEIL
                     RDFIHRLS"
     gene            complement(3993..>4556)
                     /locus_tag="PLW16_08985"
     CDS             complement(3993..>4556)
                     /locus_tag="PLW16_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPB71082.1"
                     /translation="WSPSWGNTQPWQVYVLTGEPLERFRQANQEQLRSGVAPAPEIEM
                     PQEWPATLKSRYVGVGKDVLTALAIPREDKAARIRYALDMFGLFGAPCLIVVCVPAAL
                     PRAYATLDVGLFLQTFCLAAAARGLATCALAASVNYPQLIRRLAPVPEDATIVIGAAV
                     GYPDADVPVNRFSRSRAELDEFTVWVG"
     assembly_gap    4557..4656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4657..5655)
                     /locus_tag="PLW16_08990"
     CDS             complement(<4657..5655)
                     /locus_tag="PLW16_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB71083.1"
                     /translation="MKRTERVFRFFTIVLFAAGMLLLTGGFVAPSAGWGPAWALSFGK
                     ADTAGAPASFADLAEKLRPTVVNISTTKVLKGRQMGPMGGELPFGRFFGNDEFFRRFF
                     GDLPEREFKQRSLGSGFIISEDGYIFTNNHVVEKADKILVKLADGKECEADVKGRDAN
                     TDLALIKIDSPGKLPYARLGNSDQARIGDWVFAIGNPFGLDHTITAGIISAKGRVIGA
                     GPYDNFIQTDASINPGNSGGPLFNLAGEVVGINTAIVAQGQGIGFAIPINMAKDILDD
                     LKSRGKTTRGWLGIAIQDITEDMREALKLEDRTGALVGDVFPGEPADNAGMKTGDVI"
     gene            complement(5704..5910)
                     /locus_tag="PLW16_08995"
     CDS             complement(5704..5910)
                     /locus_tag="PLW16_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5872..5874),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HPB71084.1"
                     /translation="MSEKVIIYGKAGUPYTQKARSAYGERAVYVDVRQDAERMQDMLR
                     HSGGRREVPVIVEGSRVVIGFGGA"
     gene            6115..7452
                     /locus_tag="PLW16_09000"
     CDS             6115..7452
                     /locus_tag="PLW16_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HPB71085.1"
                     /translation="MLKIRGIVPLVVLALIAGCGWSDAAETIRDLRTLPQDARAYLSP
                     ASEARPAVTAEDQDRLDARYNEQFLSPWRQTQPRHGVEELDRSFKRYRKAPGYAENGR
                     PHTPEWIDELRASAQFETYPNAVYAGMTIRPTDVRVLPTKKPRFGGLNGYPFDLFQQT
                     ALPVNTPVCVVHATRDKDWLLVETAWFVGWIPAEDVARMNEAGIRAWERGPYVTILRD
                     GVPVYGAADGMFLFHAPLGAMFPLAKARDDGWEVFVTVANEKREAVTRLAFAASGPVT
                     RKPLPLTPRNIAGQINELIREPYGWGGLNGSRDCSATLRDLFAPFGIWLPRNSSEQAR
                     QGGGVINLKRLRPEIRKEQILSRGIPFLTLLWEPGHIMLYVGSVQDEILVFHNFWSVK
                     ARDRKGRVQQKVIVGQAAITTLHPGEELEGVTVRTSNRLHALWAMTLMVPPAS"
     gene            complement(7484..8827)
                     /locus_tag="PLW16_09005"
     CDS             complement(7484..8827)
                     /locus_tag="PLW16_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB71086.1"
                     /translation="MKRLLLISPVGQKSGYLLSRITTFPPLGLAYVAAATPSDWDVRI
                     LDENFETFAFEEADLVGITSFTSNIRRAYEIARVYRERNIKVVMGGIHVSMNPDEALR
                     YADAVVVGEVEGIWKQVLDDFGQGRLAPKYSGPQIDLTRSGITPRRDLLHPNYLWQAV
                     QTSRGCPFDCDFCSVSRHLGKHYRQRSAAHVLDELSQCTGDYVVFVDDNLVGHTREDK
                     HRATDLFQGMIQRKLNKKWWMQTSINAADDERLIELAARAGCMFVFIGFESISEDMLR
                     GMNKRANLKIGIENYRKVVHAFHRHGIAVLGAFIIGNDRESPDYYKKLADFLVHSGID
                     MVQISILTPLPGTRLMERMQNEGRLIYQDFPADWDKYRFSYVVHRMNGLEPETVYTGN
                     NYIKGQLYSFPTAQYRLMKSLWSLKRIPNFYTVVKLNQALKKSWQGSHYQGKYPA"
     gene            complement(9050..>9279)
                     /locus_tag="PLW16_09010"
     CDS             complement(9050..>9279)
                     /locus_tag="PLW16_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71087.1"
                     /translation="APLRMGDPLRRGVQSDDRPARLNQVVKDRGRLARTAAQIEEALS
                     RLDPAPSGLSAYVFGTGLDSRRYVSSSCSMR"
BASE COUNT         1601 a         2851 c         2971 g         1756 t
ORIGIN      
        1 gcctaccagg tggatgaaat catggccttc accctgcgca cgttcggcca ggggacgaag
       61 gtggcggtcg agatttccct gatggcggcg gatgccgggc tcgtccggac ggacgaggac
      121 gtgatcgccg ttggcgggac ggtgagcggc tgcgatacgg cgcttctcct ccagcccgcc
      181 aacagtgccc atgtcctcga tctgaagatc cgggaggtga tcgcgaaacc gtccgtgttc
      241 taacgtcgcg ggaaggaccc gggcaggcat cgacgccggc gggggcggta tcggtcaggc
      301 ggttctgttc cggttcgcga agaatcccac caggaattcc gcatccacgg gcgacagatc
      361 gaactggaaa accgcctttt cgacgagggc cgggagggac tggttgggat tgtcttctat
      421 ggcggcggag atccaggtga tagcctgttt caggtcttcg ccttcgggta tgagtgttgc
      481 catgtctgct gtcctctttg taaaagctgc gcggcggagt cgcacgccga ctcgaaaagg
      541 gtccggagcg cggcgtcggc cgcgatgatc acaggcctca cgacggggcc tccaacgact
      601 gaacggcgaa gcattcgcag aagcggccct tccccgtggt gatgccccgg tagcggttga
      661 gtccggcggc ggcctcgtcg taaggaatgc cgtcgagctg cgatcggtgc cgtctcagcg
      721 ccgccagctt caggtccatg aacgcgctga tgtcctcgac gcagggaacc gccgtcagcg
      781 gggtccacac ctcgtaacac agaatcgtcc ccaccgccca gggctctccg gggctttcgg
      841 agaaccacgg ccctccggcg cggcggcagg cctccacgca tagccggtgc gtggcggcgt
      901 ggtcggggtg tgtctcctgt gcgtggggga tgtagaggat atcgggccgg atacggcgaa
      961 caatctccac gaggccgacg aggttcgatc gatcgtacgc caagtagccg tcggggtttc
     1021 cgaggaagat cagatccgtg atccccgcaa aggccgcggc ctcgcgggct tccgcttccc
     1081 gcacggcggc cagtgcctct ctcgtgcggc cgaggccccc cgcttccccc gaggtcatat
     1141 agaccaccgt gacggggttt ccccggcgga tgtgcttcag aaggctgccg ccgcacccga
     1201 tccactcgtc gtcgggatgg ggggcggcga cgaggatgcg ccgcatgttc ctgtttctcc
     1261 ctgaaggctt cgggacgaat cctcacgacg aacgtctgta cgtatgttgt cagggtcgtt
     1321 cccgaacgtc cgcctcctca gcggaagccg ttcttgccgg tccgggcgcc tccccgcggg
     1381 agggcaacgg gcgggttcaa aaattccggc aggggtaggc aaagctggtt ccattgatga
     1441 atgcagcggc ggcaacgggg gctctgatcg gcacgggcct cacaggcgtc gagatcgatg
     1501 aagtagtgat gacgatcgca ccaccggtgt tctttgccgg accgcgcttt catggttcgt
     1561 tccgacgggc ctttcccccc tggaaacgaa accccgcctc cctgatcatg aagggcacgc
     1621 ggggcggggt ctcttgtcct tccttgcgtc cgtcacacgg agatcaggcg cgtttcttgg
     1681 cttttgccgc cggtttcttg ggtgcggcct ttttgacggc ggccttcggc gccgctttct
     1741 tggccggctt gactttcgtc ttggcctttg ccgccggttt ggctgcgggg gctttcaggg
     1801 ccggcgcctt ggctgccggc tgggccaggg cctttttctt ggctttcgcg gcagcggcct
     1861 tgcccctgcc catcggtgcc ttgcagcaca ccagatccgc ggtcgcgcag ccgcacactt
     1921 catcgacgac gacgatcaga ccgcagacct cacaactgta catgtcccct tttcttgcct
     1981 ttgccatgcc tgtctcctcc cttagataaa atggttttta tctccgattc gaaaacatac
     2041 ccgttgcaaa gatccgattt tcttttatcc gaagcggacc gatatttcaa gaaaattatc
     2101 gtcgcgcgtg tcgggacggg cgcgacacat cgcggtagag cgcggcgtag gccttcattt
     2161 cccggtcggg tcgatggaag gcggcgatcc gccgtcgcgc gttttttccg acggccgccc
     2221 atcgctccgg ctgttcaagg atggtcagga gatgccgctc cagctcttcc cggttgcgga
     2281 acaggagtcc ggccgcgccg gctccgagat gggcgtttcc cggaatgtcc cgtgcgatca
     2341 cgatgcgtcc gaaactcagg gcctccagga tggcgttgga ttccgactcg gagcgggacg
     2401 tgttcagaac gaggttgctc tgctcgagga gggaggggac ctgattccgg ggaacctcgc
     2461 cgagatagcg gatccaggga tgaatacgca gggccttgtg cagggcctcc gtcgccgccg
     2521 gctccaggag gggaccggcg atgagcagct ggaacgtgcg atggtggcga cggaccttgt
     2581 tcaatgcggc gacggcgtag tccatctgct tgacctgtct caatccgccc agcatcagaa
     2641 tcaccggcgt gtcgccggac aggccgagcc cggcccgata atcgtaatcc ctgcgctccg
     2701 gaaaatccac ggactgatgg atgacacgga tcttgtcccg ggggatcccc aggcgattga
     2761 gccgggggag aagcgtttcc tgaaagaccg tcaccgccgc cgaccggttc agaacctccc
     2821 gcatgacccg tcgcttgtcc gggtcggcga aatccaggtt gatgtccgtt cccgtcaggg
     2881 tggtgatcag ggggacgccg agctgttctt gcaggcgcag tcccgtggtg ccgcccttga
     2941 cggcgtgaaa tgcgtgcaga agatcgggtg cggcggaccg gacgcgtttc cggagggtct
     3001 ccggcgacgt ccgcgacagg tcgaggatct cgcaggaaat attccgccgg gaaagcagac
     3061 gggcgatgcg atccaccgtg acggcgtttc ccgtacaccg gggaaaggcc gtcggagcga
     3121 gcatcaggac gttcatgtca ggacaggcgg tggatgaagt cacggaggat ttccgtgacg
     3181 gtccggggct gctccatggg gatcagatgt ccggcgtccg ggatcagccg gtattcgggc
     3241 gtgtgaggga tgtgggacag ggcctccttc aggtcgatga aggcgcgatt ttcgcttgct
     3301 tctccttcga gcatcaggac cggacacgtc acgcggggca ggacgggcca cggatcgtac
     3361 tgcatgcccc ccatgaaaag cgccgcctcc cgctcggggg cgcaggtcag cgtcagcccg
     3421 cccgcgtcgt cgtccatcat cccgtagcgg atgtagaggc ggagcatctc ctcgtcccat
     3481 ttctcgaaca gggcgcggga gcggagataa gcgagggccg cgttctcgtc cggccagtgg
     3541 ttcgtgcggc ggatggcctt ggaggccagg ggatggtctt ccaccgtgat gagggtccgg
     3601 taatagtcgc gggggagaaa gatgggctcg acgaggagca ttccccgcgc ggcgagtccg
     3661 cactcggcgt gggcaatggc caggacggtc gcccccatcg aatgccccac caagagcggg
     3721 tggtcgagat ccaggcgacg gcagaattcc gtcaggtccc gggcgatctc gagccagctc
     3781 aggccgccgc ggttcggatc cgtgtcccgg tggtcgcaga agtagggggc gattacccgg
     3841 aaggacgggg ccagcttgcg ggcgacggga tgccacagcc agggcagaaa cccggtggcg
     3901 tggagcatca cgatcgccgg accgtcaccc tcgtacagca gataggacag cgaggcgtcg
     3961 ccgatgtcct gtgcgagaat gcggggctcc attcaaccga cccagaccgt gaattcgtcg
     4021 agctcggcgc gggagcgcga gaaccggttg acagggacgt cggcgtccgg atagccgacg
     4081 gccgcaccga tgacgatggt cgcgtcttcg gggaccggcg cgagccggcg gatcagttgc
     4141 ggatagttga cggacgcggc cagggcgcac gtcgcgagtc cgcgcgccgc ggcggccagg
     4201 cagaaggtct gcaggaacag gccgacgtcg agcgtggcat aggcgcgcgg cagagcggcg
     4261 gggacacaca cgacgatcag gcacggcgcg ccgaacaggc cgaacatgtc cagggcgtaa
     4321 cgtatccggg cggccttgtc ttcacggggg atggcgaggg cggtgaggac atccttcccc
     4381 accccgacat agcgggattt gagagtcgcc ggccactcct ggggcatctc gatttccggc
     4441 gccggcgcca ccccgctccg gagctgctcc tgattggcct gacggaagcg ctcgaggggc
     4501 tcgcccgtca ggacgtaaac ctgccagggc tgggtgttgc cccacgaggg ggaccannnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatc acgtcgccgg tcttcatccc
     4681 cgcgttgtcc gccggctctc ccgggaagac gtcgccgacc agggcgcccg tccggtcctc
     4741 gagcttgagg gcctcccgca tgtcctccgt gatgtcctga atcgcgatgc ccagccatcc
     4801 gcgggtggtc tttccccggc ttttcaggtc gtcgaggatg tccttggcca tgttgatggg
     4861 aatcgcgaag ccgatcccct gcccctgggc gacgatggcg gtgttgatgc cgaccacctc
     4921 tccggccagg ttgaacagcg gaccgccgct gttgccgggg ttgatggacg cgtcggtctg
     4981 gatgaaattg tcgtagggac cggcgccgat gacccgcccc ttggcgctga tgattcccgc
     5041 cgtgatcgta tgatcgagac cgaacggatt gccgatggcg aagacccagt cgccgatccg
     5101 cgcctggtcg gaattcccca ggcgcgcgta cggcagtttc cccggggaat cgatcttgat
     5161 gagggcgaga tccgtattgg cgtcccgtcc cttgacgtcc gcctcgcact ccttgccgtc
     5221 ggccagtttc accaggatct tgtcggcctt ttccaccacg tggttgttgg tgaagatgta
     5281 gccgtcctcg gaaatgatga atccggagcc caggctcctc tgcttgaatt cacgctccgg
     5341 caggtcgccg aagaagcgcc ggaaaaactc gtcgttgccg aaaaagcgcc cgaagggcag
     5401 ctcgcccccc atcgggccca tctgccggcc cttgagcacc ttggtggtcg agatgttgac
     5461 gaccgtgggg cggagcttct ccgccaggtc ggcaaaagac gcgggggcgc ccgccgtatc
     5521 ggcctttccg aaggacaggg cccatgccgg cccccatccc gcggacgggg cgacaaatcc
     5581 tccggtgagc agaagcatgc ctgcggcgaa caaaacgatc gtgaaaaatc gaaaaacccg
     5641 ttccgtccgt ttcatggtgt ccctctcctc tttgacgccg gtgcccgatc ggcttcagac
     5701 ccctcaggcc ccgccgaatc cgatcacgac cctggaccct tccacgatga ccggcacctc
     5761 ccgacggccg ccggaatgcc ggagcatgtc ctgcatccgc tccgcgtcct gtcggacatc
     5821 gacatagacg gcgcgctccc cgtaggccga cctggccttt tgggtgtagg gtcagcccgc
     5881 ctttccatag atgatgacct tttccgacat cggggtcccc gggcgccgtc catcatcggg
     5941 atgatcctgt cctcccgcgc ggggaggacg ctcagcgccg tttttctttt ttataccaat
     6001 ctttttttct ttccgccaca caaaatccga ccgcgcctca ggtggccggc acttgacgtg
     6061 caacgggcaa ccgcatatag tgcccgtcat cagcgtcggc ttttggagga gatcatgctc
     6121 aagatcagag ggatcgtacc gctcgtcgtt ctggcgctga tcgcggggtg cgggtggagt
     6181 gacgcggccg agacgatccg ggatctccgc accctgcccc aggatgcgcg tgcctatctt
     6241 tcccccgcaa gcgaggcgcg gcccgccgtc accgcggaag atcaggaccg cctggacgcc
     6301 cggtataacg agcagtttct ttccccctgg cgccagacgc aaccccggca tggcgtcgag
     6361 gaactcgatc ggtctttcaa gcggtaccgg aaggcgccgg gctacgcgga aaacggccgc
     6421 ccccacacac ccgaatggat cgacgaactc agggcctcgg cccaattcga aacctatccg
     6481 aatgccgtct atgcggggat gacgattcgg ccgacggacg tccgagtctt gccgacgaaa
     6541 aagccgcgct tcggaggatt gaacggttat ccgttcgatc tcttccagca gacggccctg
     6601 ccggtcaata caccggtttg cgtggtgcac gcgacgcgcg acaaggactg gctgttggtg
     6661 gaaacggcct ggttcgtcgg ctggattccc gcggaggatg tcgcccggat gaacgaggcg
     6721 ggcatccggg cttgggagcg ggggccctac gtgacgattc tccgcgacgg ggttcccgtg
     6781 tacggggcgg ccgacgggat gttcctcttc catgcacccc tcggggcgat gtttcccctg
     6841 gcgaaggccc gggacgacgg ctgggaggtt ttcgtgacgg tggcgaacga aaagcgcgag
     6901 gccgtcaccc gattggcctt tgcggcttca ggtccggtca ccagaaaacc tctgccgctg
     6961 acaccgcgga acatcgccgg gcagatcaac gagttgatcc gtgagcccta tggctggggt
     7021 ggcctgaacg gcagccggga ctgttcggcg accctgaggg atctcttcgc cccgtttgga
     7081 atctggctgc cccgcaactc ctccgaacag gcccgtcagg ggggcggggt catcaatctg
     7141 aagcggctca ggccggagat ccggaaggag cagattctgt cgcggggcat cccctttctg
     7201 acgctgctgt gggaaccggg ccacatcatg ctgtacgtcg gcagcgtcca ggacgagatt
     7261 ctggtgtttc acaacttctg gtccgtcaag gcccgggacc gcaagggccg tgtccaacag
     7321 aaagtcatcg tcggtcaggc ggccatcacg acccttcatc ccggcgagga gttggaaggg
     7381 gtaacggtcc ggacgtccaa ccggctccat gcgctctggg ccatgacgct gatggttccc
     7441 ccggcgtcgt gagcgtttgc cgggagaaat ccggttcctt gcttcaggcc ggatattttc
     7501 cctggtaatg ggacccttgc cagcttttct tgagggcctg attcaacttc acgacggtgt
     7561 agaagtttgg aatccgtttc aggctccaga gggatttcat caggcgatac tgagcggttg
     7621 ggaatgagta gagttgtccc ttgatgtagt tgttgcccgt gtagaccgtt tcgggttcaa
     7681 ggccgttcat ccggtgcacg acgtacgaga accggtattt gtcccagtcc gccgggaagt
     7741 cctggtagat cagccgcccc tcgttttgca tccgctccat cagccgggtg ccgggcaggg
     7801 gggtcaggat gctgatctgt accatgtcga tgcccgaatg aacgaggaaa tccgccagtt
     7861 tcttgtaata atcgggagac tcccggtcat tgccgatgat aaacgccccc agcacggcaa
     7921 tgccgtgccg gtgaaacgca tggacgacct ttcggtaatt ctcgatcccg atcttcaaat
     7981 tggcccgctt gttcattccc cggagcatgt cttcgctgat ggattcgaag ccgatgaaaa
     8041 cgaacatgca cccggcacgg gccgccaatt cgatgagtcg ttcatcgtca gcggcattga
     8101 tggaggtctg catccaccat ttcttgttca gtttgcgctg aatcatgccc tggaacaggt
     8161 ccgtcgcccg gtgcttgtct tcccgggtgt gtcccaccag attgtcatcg acaaaaacga
     8221 catagtcccc cgtgcactga gacagctcgt ccaagacatg cgctgcgctg cgctgacggt
     8281 aatgcttccc cagatggcgg gagaccgaac agaaatcgca atcgaaggga cagccgcggg
     8341 aggtctgcac cgcctgccac aggtaattcg ggtgcagcag atcgcgccgc ggggtgatgc
     8401 ccgagcgcgt cagatcgatt tgagggccgc tgtatttcgg ggccagccgc ccctgcccga
     8461 agtcgtccag cacctgcttc cagatcccct ccacttctcc cacgaccacc gcgtccgcgt
     8521 aacgcagggc ctcgtcggga ttcatggaga cgtggattcc ccccatgacc accttgatgt
     8581 tgcgttcccg atagacgcgg gcgatttcgt aggcccggcg gatgttgctc gtgaaggacg
     8641 tgatgccgac caggtccgct tcttcgaatg caaacgtctc gaaattttca tcaaggatcc
     8701 ggacgtccca gtcgctcggc gtcgccgccg ccacgtaagc cagccccagc ggcggaaagg
     8761 tggtaatccg gctcaacagg tatccgcttt tctgccccac cgggctgatc agtaaaagtc
     8821 gtttcatcgt gtccgctctt acctcaatga catccatgaa tgcaaccgtt aagtggcgca
     8881 acggatcgcg cccccggtct tccgaccctt ttccatggat cagggtgcgg cccgtcgaaa
     8941 aatttgacaa ccgccggcaa agccgttaaa gggggtgcgg caggcatgcc atttctttgg
     9001 caaggacaaa ccccatgcag gagatgcctt tggcaatacc gcgggatttt tacctcatgg
     9061 agcatgaaga ggaaacgtac cgtctcgaat caagacccgt cccgaagaca tacgccgaca
     9121 ggcccgatgg tgcgggatca agccgggaca gcgcctcctc gatctgggct gcggtccggg
     9181 caagacgacc gcgatccttc acgacctgat tcagccgggc gggtcgatcg tcggactgga
     9241 cgcctcgccg gagcggatcg cccatgcgca acggtgcta
//
LOCUS       DAOOLJ010000070         8726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_9441_length_8726_cov_18.066659, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000070 DAOOLJ010000000
VERSION     DAOOLJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9441_length_8726_cov_18.066659"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /gene="ftsH"
                     /locus_tag="PLW16_09015"
     CDS             complement(<1..1672)
                     /gene="ftsH"
                     /locus_tag="PLW16_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB71088.1"
                     /translation="MKKHYKFSIWYVLLGIWVVLLVQSYIASMLAVPTIPYSQFLNQL
                     KAGNITEVAITANQIQGKMKVDPNNPGEQKAFKTIRVDPELSKTLEQYPVIFKGEIES
                     TWMRDLFSWVFPLLLFVGIWYFLMKRMGGQQAGFMTLGKNKARIYMENELNVTFADVA
                     GVDEARQELVEVIEFLKTPGKFTDIGGRIPKGILLVGPPGTGKTLLAKAVAGESGVPF
                     FSLSGSEFVEMFVGIGASRVRDLFVQAKEKAPCIIFIDELDALGKARGFGAMGGHDER
                     EQTLNQLLVEMDGFDPKVGVILMAATNRPEILDPALLRAGRFDRHVLVDRPDKQGRED
                     ILKVHMKKVKAAPEVDLGILANMTPGMVGADLANLINEAALLAVRAGRSVVAMKDFEE
                     AVERIIAGLEKKNRLINPRERETVAYHEMGHALVALSLPGTDPVQKISIIPRGISALG
                     YTLQVPTEDRFLMKKTELENKIATLLGGRAAEEIIYGDVSTGAHNDLSRATDIAKSMV
                     REYGMSEALGQVYLARDKRPQFLDIGLGEGGDYSPATAEAIDREVREII"
     gene            1888..2868
                     /locus_tag="PLW16_09020"
     CDS             1888..2868
                     /locus_tag="PLW16_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB71089.1"
                     /translation="MKRSLWRYAVLCTIGLMLVPACGWAAEVVLYSSNPPELLDMVSQ
                     GFEKKTGIKVSTVRLGTGEAMKRIAAEKNKPLCDVFWSGDVAVLDNAKQYFLPYKSRE
                     ARALPKNYIEKEGRWTSSNAHVMVIMYNKSLVREPEKPKAWKDLFLPKWKGKVVIANP
                     EKSGSAYAQVYGIYKLYGWDGLKKLIDNAKILDSSSLIYKGVAGGEYPIGVTMEYAAY
                     RYVAGGSKDVGIVYAADGAIVAPEGAAVILNSPHPQEAKKFFDYLISKPVQEEVFEKF
                     YRRPARTDAKTIAGLPALKKIRVLKKFDPLEANVLEKDILKQWKEIVLSR"
     gene            2876..3946
                     /locus_tag="PLW16_09025"
     CDS             2876..3946
                     /locus_tag="PLW16_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB71090.1"
                     /translation="MQVELRDIRKSFGRLTVIDGVSLEIEEGHLFTLLGPSGCGKTTL
                     LKIVAGFHAPDAGQVLFGGTRVNAVPPHKRETGMVFQTYALFPHLTVFDNVAYGLRTR
                     RTSQDQIRKKVAEVLENVQLDGLEARFPSQLSGGQQQRVALARALVFSPRILLMDEPL
                     SNLDARLRISMREEIKRIQKRLGITTIYVTHDQEEAMAISDRVAILNGGRLEQIGTPW
                     EIYAAPRNRFVAQFMGSSNILDLDVTGYDAGRARAQARTGDQVFHLCSGKPLSGPILV
                     ALRPEWIDILPDGAEAPVNAFTGTVVSAMFVGSLVRYGVRCLGDRLLTIDAADPHKRG
                     IKGEGEPIRLGFDAERPVVIEP"
     gene            3982..5637
                     /locus_tag="PLW16_09030"
     CDS             3982..5637
                     /locus_tag="PLW16_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB71091.1"
                     /translation="MNIGRHLNTWNVILVLVFIVLFFAVLYPVLYIFKASFMDPETGA
                     FSLNSYQTFFKYPYYLRCLRNSLFVSALATGFALVIGIPFAFFLSRYDLPGKDVIKTL
                     GTLPLILPTFIGAEAWIMLLGRNGLLANTLGRFGLEAPTIYGWKGIVLVYTLQFFPYV
                     FLMVSSAVNAIDRSLEEAATSLGASRLRAFFTVTLPLVTPAILSGALVVFYLSIENFG
                     VPILIGEDFRVLSVQAYNEFISEMGGNPSMAGALSMILLSLTLLLTVVQKYWTERKTY
                     AITSVQRTAVKRLSLLPTALIWVFCAGLVFIALFPFLVVVVAAFTKAHGPVMYYGQFT
                     LDHFIKAFTIAPRPILNSFFLATAATLIGIVFGLVVSYLLVRRRGTVSYVLDVLTMLP
                     LAIAGTVQGIALAVTYNKGLVVLTGTWMILVLAYFIRKVPFSIKTTSALLHQIDRNIE
                     DASVNLGVPPFRSFLKVILPVMMPGIIAGAIIMWVTTLAELSSTIVLYYGPWATMTVE
                     IFQRINSGDFGPASAFATVLIVSVLIPLFILHKLLGRDLTTAL"
     gene            5762..6211
                     /locus_tag="PLW16_09035"
     CDS             5762..6211
                     /locus_tag="PLW16_09035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPB71092.1"
                     /translation="MERYDVLPPALKQALMKKMKTDSPFWTLLGMELLDARKGWARMR
                     LPFDRKLVHPMGIAHGGAIFSIADSAVAMALIGMVDRGDAFVTVEMKLNYVLPFASGE
                     ITAEARIIHQGTKTALGDVEVRNDRDELVAKGLATYMILKGGAQANG"
     gene            6417..7001
                     /locus_tag="PLW16_09040"
     CDS             6417..7001
                     /locus_tag="PLW16_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB71093.1"
                     /translation="MDFKNSKTRTNLMNSFAGESQARNRYTFFASAAKKEGYEQISEI
                     FTDTANNEKEHAKLFYKHIVDLSGGEDAIQVNGTYPVILGDTLANLKSAAAGENEEWT
                     ALYPEAAKIADAEGFPKVAETFRQVAEAEKSHEDRYLALAQNIANGEVFKKKAKIKWR
                     CRNCGRIIESQEAPKVCPTCQHPQDYFEVKAENY"
     gene            7446..>8726
                     /locus_tag="PLW16_09045"
     CDS             7446..>8726
                     /locus_tag="PLW16_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB71094.1"
                     /translation="MGSLICDVRDQKFVLYELLKIEELCKTEKYADFSRETFDMTLAE
                     AEKFATEVLFPTMAEGDRVGCKLENGLVKVPPCFRRPFQLYQEGGWSTMSVSQEAGGQ
                     GFPLVMSSAAKEWFIHNFGMMCYPYLTEGAAHLIEVYGTEAQKRKYMDKMYACEWGGT
                     MALTEPGAGSDVGNLKTKAIRQPDGTFRLQGTKQFITAADHDLVPNIVHPVLARIEGD
                     PAGTGGISIFLVPKYLVNDDGSLGRRNDYTIGAIEEKMGLHGSATCVMNLGDNGDCYA
                     ELLGEERQGIKVMFQLMNEARIGVGIQSLSLGGIAYLHAVNYAKERLQGSSLLEMKNP
                     EAPRVPIIQHADVRRMLLWMKAHVEGFRALTYYTAYCEDIVHASADEAHRDKCKGIIE
                     VLTPIVKAYCSDVAFKVTELAIQTYGGYGYCSEYP"
BASE COUNT         1634 a         2823 c         2643 g         1626 t
ORIGIN      
        1 cgatgatctc ccggacctcc cggtcgatcg cctcggccgt cgccgggctg tagtctccac
       61 cctcgccgag gccgatgtcc agaaactggg ggcgcttgtc ccgggccagg tagacctgac
      121 ccagggcttc gctcatcccg tattcccgga ccatgctttt ggcgatgtcg gtggcgcggg
      181 acagatcgtt gtgggccccc gtggagacgt cgccgtagat gatctcctcc gccgcgcggc
      241 caccgagcag ggtggcgatc ttgttttcca gctccgtctt cttcatgaga aatctgtctt
      301 ccgtgggcac ctgcagggta tagcccaggg ccgagatgcc ccgcgggatg atcgagatct
      361 tctggaccgg gtcggtgccg ggcagggaca gggcgaccag ggcgtgcccc atctcgtggt
      421 aggcgaccgt ttcccgctcc cgggggttga tgagacggtt tttcttctcc aagccggcga
      481 tgatccgctc caccgcctct tcgaaatcct tcatcgccac gacggaccgg cccgcccgga
      541 cggcgagcag ggccgcttcg ttgatcaggt tggcaaggtc cgccccgacc atccccgggg
      601 tcatgttggc gaggatcccc agatcgacct cgggggccgc cttgaccttt ttcatatgga
      661 ccttgagaat gtcctcccgc ccctgcttgt ccgggcggtc gacgaggacg tgccgatcga
      721 agcggcccgc ccggagcagc gccggatcca ggatctccgg acggttcgtc gcggccatca
      781 ggatcacccc caccttggga tcgaagccgt ccatctcgac caggagctgg tttaaggtct
      841 gctcccgctc gtcgtggccg cccatggccc cgaatccccg ggccttcccc agggcgtcga
      901 gttcgtctat gaagatgatg cagggggcct tttcctttgc ctgcacgaac aggtcgcgta
      961 cccgggaggc gccgatcccg acaaacatct cgacgaactc cgatccggag aggctgaaga
     1021 aggggacgcc gctttccccc gccacggcct tggccagcag ggtcttgccc gttcccgggg
     1081 ggcccacgag caggatgccc ttcgggatcc gccccccgat gtcggtgaac ttccccggcg
     1141 tcttaaggaa ttcgatcacc tcgaccagct cctgcctcgc ctcatccacg ccggccacgt
     1201 cggcgaaggt cacgttgagc tcgttctcca tgtagatccg ggccttgttc ttccccaggg
     1261 tcatgaagcc cgcctgctgg ccgcccatcc gcttcatcag gaaataccag atcccgacga
     1321 acagcaggag gggaaacacc caggagaaca ggtcgcgcat ccaggtggat tcgatctcgc
     1381 ccttgaagat caccgggtac tgctccaggg ttttggacag ttccggatcg acgcggatgg
     1441 tcttgaaggc cttctgttcg cccgggttgt tcggatcgac cttcatcttt ccctggatct
     1501 ggttggccgt gatggccacc tcggtgatgt tgcccgcctt gagctggttc aggaactggc
     1561 tgtaggggat cgtcggaacg gccagcatgg aggcgatata gctctggacg agcagcacaa
     1621 cccagatgcc gagcaggacg taccagatgg aaaatttata gtgtttcttc atcatgccct
     1681 cccgcatgga tgacgtttca tgaacggatt tcaacctcgc gctaggattc aatataacag
     1741 ataagcaatc caaagaaaac gagtttccgc ccacagccct ttgtcgttga catccccggg
     1801 tgttttccct ataaaagtgc agagccgtgc cggatcgacc ggcacgttgg cgaccgaacc
     1861 catgggacgt ttacgggagg taagcttatg aagcggagtc tttggcggta tgcggtgttg
     1921 tgcaccatcg gcctgatgct ggttccggcc tgcgggtggg cggcggaggt ggtcctgtac
     1981 tcctcgaacc cgccggagct gcttgacatg gtcagccagg gttttgaaaa gaagacgggg
     2041 atcaaggtat ccaccgtccg cctcgggacg ggagaggcca tgaagcggat cgccgcggaa
     2101 aagaacaagc cgctctgcga cgtcttctgg agcggcgacg ttgccgttct cgacaatgcc
     2161 aagcagtact tcctccccta caagtcccgt gaagcccgcg ccctcccgaa gaactacatc
     2221 gagaaggaag gtcgctggac gagctccaac gcccatgtca tggtaatcat gtacaacaag
     2281 tccctcgtca gggagccgga aaaaccaaag gcctggaagg acctcttcct gcccaaatgg
     2341 aagggcaagg tcgtcatcgc caatccggag aaatccgggt ccgcctatgc ccaggtgtac
     2401 ggcatctaca agctctacgg ctgggacggc ctgaagaagc tcatcgataa cgccaagatc
     2461 ctcgacagct ccagcctgat ttacaagggc gtcgccggcg gggaatatcc gatcggcgtc
     2521 acgatggaat acgccgccta ccgctacgtg gccggcgggt ccaaggacgt ggggatcgtc
     2581 tatgccgccg acggcgccat cgtggccccc gagggcgcgg cggtgatcct gaacagcccc
     2641 cacccccagg aggcgaagaa gttcttcgat tacctgatca gcaaacccgt ccaggaggag
     2701 gttttcgaga agttctaccg ccgtccggcg cggacggacg cgaaaaccat tgccgggctt
     2761 cccgccctga agaagatccg ggttctcaag aagttcgatc cgctggaggc gaacgtcctt
     2821 gaaaaggaca tcctgaagca gtggaaggaa atcgttctct cgcgataaag aatgcatgca
     2881 ggttgaactc agggacatcc gcaaatcctt cggccggctc acggtcatcg atggcgtgag
     2941 cctcgagatc gaggaggggc atctgtttac cctcctcggt ccgagcggtt gcgggaagac
     3001 gaccctcctg aagatcgtgg ccggttttca tgcccccgac gcggggcagg tcctcttcgg
     3061 cgggacgcgc gtcaatgccg tcccccccca caaacgcgaa acggggatgg tcttccagac
     3121 ctacgccctg ttcccgcacc tgaccgtctt cgacaacgtc gcctacgggc tgcggacgcg
     3181 caggacctcg caagatcaga tccggaaaaa ggtggcggag gttctggaga acgttcagct
     3241 cgacggcctg gaggcgcgct ttcccagcca gctctccggc ggacagcagc agcgcgtcgc
     3301 cctcgcgcgg gcgctggtgt tctcgcccag gatcctcctc atggacgaac ccctgtcgaa
     3361 cctcgatgcg cgtctgcgga tcagcatgcg cgaggagatc aagcgcatcc agaaacgctt
     3421 gggcatcacg acgatctacg tgacgcacga ccaggaggag gccatggcca tctccgaccg
     3481 cgttgcgatc ttgaacggcg ggcggctcga acagatcggg acgccctggg agatctacgc
     3541 cgccccccgg aaccgcttcg tggcccagtt catggggagc agcaacattc tcgatctgga
     3601 cgtcacgggc tacgatgccg gacgtgcccg cgcccaggcc cgcaccggcg atcaggtgtt
     3661 ccacctttgc tcgggaaagc ccctgtccgg cccgattctg gtcgccctgc gccccgagtg
     3721 gatcgacatc cttccggacg gggcggaagc cccggtgaat gcgttcacgg gaaccgtcgt
     3781 cagcgcgatg tttgtcggct ccctggtccg ttacggggtg cggtgcctgg gggaccgtct
     3841 gctcacgatt gacgcggcgg acccccataa gcggggcatc aagggcgagg gggagccgat
     3901 ccgcctcggc ttcgacgccg agcggccggt cgtgatcgaa ccctgaacgg acccacagcg
     3961 tcccgaggcg ctgcccgaag catgaatatc ggacgtcacc tcaatacctg gaatgtcatc
     4021 ctggttctgg tcttcatcgt cctgttcttc gccgtcctgt atcccgtcct ctacatcttc
     4081 aaggcgagtt tcatggaccc ggagacgggc gccttttcgc tgaacagcta ccagaccttt
     4141 ttcaagtatc cctattacct gcgctgcctg cgcaacagcc tgttcgtcag cgccctcgcc
     4201 acggggttcg cgctcgtcat cgggattccc ttcgcgttct tcctctcccg ctacgacctg
     4261 cccgggaagg acgtcatcaa gactctggga accctgccgc tgatcctgcc gaccttcatc
     4321 ggggccgagg cctggatcat gctcctggga cggaacggac tgctggcaaa caccctgggc
     4381 cgcttcggcc tggaagcgcc cacgatctac ggctggaagg gcatcgtcct cgtctacacc
     4441 ctgcaattct tcccctacgt ctttctgatg gtatcgtccg ccgtcaacgc catcgaccgc
     4501 tccctcgagg aggcggccac gagcctgggc gcctcacgtc tgagggcttt cttcaccgtc
     4561 accctgccgc ttgtcacccc ggccatcctg tccggggccc tggtcgtctt ctacctgtcc
     4621 atcgaaaact tcggggtgcc catcctgatc ggcgaggact tccgggtcct gtcggtccag
     4681 gcctacaacg aattcatcag cgaaatgggg ggcaatccct cgatggcggg ggcgctaagc
     4741 atgatcctcc tgtcgctcac gctgctcttg acggtggtgc agaagtactg gacggagcgc
     4801 aagacctacg ccatcacgtc cgtgcagcgg acggcggtga aacggctttc gctcctcccg
     4861 accgccctca tctgggtctt ctgcgccggc ctcgtcttca tcgccctctt tccgttcctg
     4921 gtcgtcgtcg tggcggcctt cacgaaggcc cacgggccgg tcatgtacta cggccagttt
     4981 accctggatc atttcatcaa ggccttcacc atcgctccgc ggcccatcct gaattcattt
     5041 tttctcgcca cggccgccac cctcatcggg attgtcttcg gcctcgtcgt cagctacctg
     5101 ctcgtccgcc gccgcggaac cgtgagctac gtcctcgacg tcctgaccat gctgcccctg
     5161 gccatcgccg gcaccgtcca gggcatcgcg ctcgccgtga cgtacaacaa gggcctcgtc
     5221 gtcctcacgg gaacctggat gatcctcgtg ctcgcctatt tcatccgcaa ggtcccgttc
     5281 tccatcaaga ccacctcggc gctcctgcac cagatcgacc ggaacatcga agacgcctcc
     5341 gtcaacctgg gtgttccccc gttccggtcc tttctcaagg tcatcctgcc ggtgatgatg
     5401 ccgggcatca tcgccggggc gatcatcatg tgggtcacga ccctggccga gctcagttcc
     5461 accatcgtcc tgtactacgg cccctgggcg accatgaccg tggagatctt ccagcggatc
     5521 aacagcggcg atttcggacc cgcctccgcc ttcgccacgg tcctcatcgt ctcggtcctg
     5581 attcccctgt tcattctgca caagctcctg ggcagggatc tgacgacggc cctctgatgc
     5641 ggtgttgacg gagaccggtc gggatagtat aagacttgcc ggaccccccg tccctccgag
     5701 tgcggggcgc gattcccacg cgcgggaagg atcgcggatc attttcaagg agtcgttgcg
     5761 aatggagcgg tatgacgtat tgcccccggc cctgaaacag gccctgatga agaagatgaa
     5821 gacggattcc cccttctgga ccctgttggg gatggaactg ctggatgccc gcaagggctg
     5881 ggccaggatg cggctcccct tcgaccgcaa gctggtccac cccatgggca tcgcccacgg
     5941 cggtgcgatc ttttccattg ccgattccgc cgtggcgatg gccctgatcg gcatggtgga
     6001 ccggggagac gccttcgtga cggtcgagat gaagctcaat tacgtgctcc ccttcgcgag
     6061 cggcgagatc accgcggagg cccggatcat ccaccaaggc acgaaaaccg ccctgggcga
     6121 cgtggaggtc cggaacgacc gggacgaact cgtcgccaag gggctggcga cctacatgat
     6181 cctcaaggga ggggcgcagg cgaacggctg aaggctgagt gcgtcgatcg atgtgccgca
     6241 gggcctctat ccaccggata ttcatgggtt tctgggaaaa tgcacgcaaa gggattgcca
     6301 gcggaccgga agaggattac attccccgca tgacgccgag cggggtcgag gtcgccggac
     6361 acgccggcgc tgcatgcacc ccaggatcga tctgaactcg agaaaggaga cccaccatgg
     6421 atttcaagaa cagcaagacg cggaccaacc tgatgaattc ctttgccgga gagtcccaag
     6481 cccgcaaccg ctacacgttc ttcgccagcg cggccaagaa ggagggctat gaacagattt
     6541 cggagatctt caccgacacg gccaacaacg agaaggagca cgccaagctc ttctacaagc
     6601 acatcgtgga tctgagcggc ggcgaggatg ccatccaggt caacggcacc taccccgtga
     6661 tcctgggcga cacgctcgcc aatctgaaat cggccgccgc cggtgaaaac gaggagtgga
     6721 cggcgcttta cccggaggcg gcgaagatcg ccgacgcgga aggcttcccc aaggtcgccg
     6781 agaccttccg tcaggtggcc gaggcggaga agtcccacga ggaccgctac ctcgccctcg
     6841 cgcagaacat cgccaacggc gaagttttca aaaagaaggc caagatcaag tggcgctgcc
     6901 ggaactgcgg tcggatcatc gagagtcaag aagcgcccaa ggtctgcccg acctgccagc
     6961 atccccagga ttatttcgaa gtgaaggccg agaattattg aggcgtgacc cgcccgacgg
     7021 ccgttccgga tccgacctcc gtcgcgatcc ggagcggcag ccggttttcc gtgtccgcac
     7081 catcgcccaa tgtcatcgtt tccgtcttcc atttccggag ggggtgtggc gctgagagca
     7141 tcgacgaaag ccctgatggc gaagcgcggc cggcgcatcg aacgacccgt ccacaacggc
     7201 gtctatcacg ccttctctca cccgtacgtc tgcgtcgcct gccacacctt gcgcttcccg
     7261 gaacgcgatt tcacccggtt caccccctgt tgcccaacat gccacgttcg gaagattgcc
     7321 ccaaaggcgg ttccggcgct acggcctgat tccacccccg tgcgggggaa tgcggcagtc
     7381 attccccccg gctggatcat gctccggccg gggggagaaa aataacaaaa ggagaggaga
     7441 agattatggg gagtttgatt tgtgacgtca gggatcagaa gttcgtgctg tacgagttgc
     7501 tcaagatcga ggagctctgc aagaccgaga agtacgcgga tttctcgcgt gagaccttcg
     7561 acatgacgct ggcggaggcc gaaaagttcg ccacggaggt cctatttccg acgatggcgg
     7621 agggcgaccg ggtgggctgc aagctggaaa acggcctggt caaggtgccc ccctgtttcc
     7681 gccggccctt ccagctctac caggaaggcg gctggagcac catgagcgtc tcccaagagg
     7741 cgggcggcca gggattcccg ttggtgatgt cctccgcggc caaggagtgg ttcattcata
     7801 atttcgggat gatgtgctac ccctacctga cggagggcgc ggcccacctg atcgaggtct
     7861 acggcacgga ggcccagaag cgcaagtaca tggacaagat gtacgcctgc gagtggggcg
     7921 gcacgatggc cctgaccgaa ccgggggccg gcagcgacgt cggtaacctg aagacaaagg
     7981 ccatccggca gccggacggg accttccgcc tccaggggac gaagcagttc atcacggcgg
     8041 ccgaccatga cctggttccg aacatcgtcc acccggtgct cgcccggatc gagggcgacc
     8101 cggcggggac gggcgggatc tcgatcttcc tcgtcccgaa gtacctcgtc aacgacgacg
     8161 ggtccctggg tcggcgcaac gactacacga tcggcgcgat cgaggagaag atgggccttc
     8221 acgggagcgc cacctgcgtg atgaacctgg gcgacaacgg ggactgctac gccgaacttc
     8281 tcggcgagga gcgccaggga atcaaggtca tgttccagct catgaacgaa gcccggatcg
     8341 gcgtgggcat ccagtcgctc tcgctcggcg gcatcgccta cctccacgcc gtaaactacg
     8401 cgaaggagcg cctgcagggc tcgtcgctcc ttgagatgaa gaaccccgag gccccgcggg
     8461 tgccgatcat ccagcacgcc gacgtccgcc ggatgctcct gtggatgaag gcccacgtcg
     8521 aaggcttccg ggcgctgacg tactatacgg cctactgcga agacatcgtc cacgcgtccg
     8581 ccgacgaggc gcatcgggac aagtgcaagg gcatcatcga ggtcctgacc ccgatcgtga
     8641 aggcgtactg ttccgacgtg gccttcaagg tgacggaact cgcgatccag acctacggcg
     8701 gctacggtta ctgctcggag tacccc
//
LOCUS       DAOOLJ010000071         8417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_9891_length_8417_cov_14.032289, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000071 DAOOLJ010000000
VERSION     DAOOLJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9891_length_8417_cov_14.032289"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLW16_09050"
     CDS             complement(<1..464)
                     /locus_tag="PLW16_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPB71095.1"
                     /translation="MRLSEERALLVVIDIQGNLARAMDEKESLFANAARIVAGAKVFG
                     LPVVVTEQTPEKLGPTIPELAPLLTGTAPIRKESFSCCAVPAFTDALAAAGRRQILLT
                     GIEAHICVYQTALDLIEAGYELHVVADAVSSRTTRNRELALQRLRREGASITS"
     gene            630..926
                     /locus_tag="PLW16_09055"
     CDS             630..926
                     /locus_tag="PLW16_09055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPB71096.1"
                     /translation="MLGLIAKLKIQEGRMDEALDLFKGLVADVKTEAGTLSYTVNKDT
                     ADPNVIVVVERYKDADALTAHSSTPHFAAFSKRIAGLLDGQMEMSILEEVASIT"
     gene            977..1744
                     /locus_tag="PLW16_09060"
     CDS             977..1744
                     /locus_tag="PLW16_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB71097.1"
                     /translation="MGNGLVLYEERDRVAILTINRPQAMNALNTDVNLRLIELLLEAE
                     RDPAIKVAVLTGAGGKAFIAGGDIKEMLGMDAMQARGYALNAKRVIDTIWNLGKPVIA
                     AINGVCLGGGLEYAMACDLRTAAETAKFALPEINIGIMPGSAGTQRLPRLIGMAKAKE
                     FCFTGSMFDAKLALELGLLNHVYPAESLMEETLALAGKIVSKSGPSLRLIKAAMNRGT
                     ETDLETASLFEIDCFGLCFSTQERQDGMRAFVEKGRK"
     gene            complement(1906..3216)
                     /locus_tag="PLW16_09065"
     CDS             complement(1906..3216)
                     /locus_tag="PLW16_09065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPB71098.1"
                     /translation="MHGFRFYRYAVSTIEHAGRFAVLLFLTLAVLLAGASEAAAKALT
                     ISNYFSPLNQKRPFRTTTRHIVLHTTEGALEGSLRKVHRFGEAHYLVAPSGEVYRIID
                     RRRIATHTGRSMWKGKSTLDDFTIGIEVVGYHDEDITTAQYASLRELLRQLKAIYRIR
                     DKDVLTHSMVAYGRPNRFHPQRHRGRKKCGMLFAHPDVRRRLGLSARPTRDPDVAARR
                     LAVADPELHRFLYRPPRMWAALIGARESQRPDERREATRVIAKGINAWNVARDRYDHR
                     TTIYEYPDGRRFRGDEIREWEAIPAGTRVRFEDVPEREQSFEGFLEIGRDGSRASDLA
                     GDLYTDSTTIYFFPSGLIRTGAELNKNASLRKLLERPPKGTRVLAGYIYGGYVQGVRS
                     ASRIAGRKWNYPSTFYRLPDGRILSGDEIDDQKIPGRTLVFLLK"
     gene            complement(3297..4310)
                     /locus_tag="PLW16_09070"
     CDS             complement(3297..4310)
                     /locus_tag="PLW16_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPB71099.1"
                     /translation="MSITTTLQHKSRNQAEGSISRFMETHYRHFNALTLRDAALSYRD
                     HINKGGKMFLAMAGAMSTAELGISLAEMIRQDKIHAICCTGANLEEDVFNLVANQTYV
                     KIPEYRNLSPSQERELLERGYNRVTDTGIPQDVMFHIEDRLKRRWREAEKTGTLKFPH
                     EFFYDLLLSGELTAHYQIDPENSWLLAAAQKRLPIYVPGWEDSTLGNVYAATCIKKEL
                     KPGSIRGGIEYLMHLAEWYQKIDTETSAGIGFFQIGGGIAGDFSICVVPMLNYDLKEK
                     VKHWAYFCQISDSITSFGSYSGAVPNEKISWGKLSVDTPRYVIESDATIVAPLLFSYV
                     LER"
     gene            complement(4686..5093)
                     /locus_tag="PLW16_09075"
     CDS             complement(4686..5093)
                     /locus_tag="PLW16_09075"
                     /inference="COORDINATES: protein motif:HMM:NF009806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HPB71100.1"
                     /translation="MIVRDIRQIIDTEREVAGKGWVSRRLLLKKDGMGFSFHETIITA
                     GTEHRFWYKNHLEAVYCVAGNGTIEDLASGEVHEVKDGVLYALNDHDCHILRGGTEDM
                     RLICAFNPPVTGRETHDGEGSYELVEERAPNAS"
     gene            complement(5093..6367)
                     /gene="ectB"
                     /locus_tag="PLW16_09080"
     CDS             complement(5093..6367)
                     /gene="ectB"
                     /locus_tag="PLW16_09080"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: protein motif:HMM:NF006733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HPB71101.1"
                     /translation="MRIFENLESRVRGYIRSFPVIFHTAKGSIMVDEQGKEYIDFFAG
                     AGTLNYGHNNEQITQALIEHLQLGGVVHGLDMATTAKKNFLEKFYNTILQPRNMEYKI
                     QFTGPTGTNAVETALKLARMVKARSNVVCFTNGFHGLTMGSLAITGNAFYRDEAYISR
                     SNVSFMPYDGYLRPDFNTLDLFRKMLADNSSGLDTPAAVILETIQAEGGINVARPEWL
                     KELAEICHQFDILMIIDDIQVGNGRSGDFFSFEEADLKPDIVCLSKSIGGGLPMAFIL
                     MRPDLDQWRPGEHTGTFRGNNLAFVASTQALCYWDTNDFSEAVKYKGTVMERELKKIA
                     ETYPELEASVRGRGMIYGLELPERGLASQVSERCFENGLIIELAGSYDQVVKFLPALT
                     IDEETLLRGIEIVGQSIGEILKKKKENLTGEL"
     gene            complement(6378..6884)
                     /gene="ectA"
                     /locus_tag="PLW16_09085"
     CDS             complement(6378..6884)
                     /gene="ectA"
                     /locus_tag="PLW16_09085"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HPB71102.1"
                     /translation="MEPHNSIQLNKPRRGDGPAMRVIARDTGVLSVNSAYYYAMMARC
                     FSDTCLVARSGEQVCGYIAALFLPAQPETLFVWQIGVSRAAQGKGIGKQMLIRLIERA
                     RPAFLEATIALDNQASLRAFQAAARQMAADATFSDKPFFTQSDMGSTDAPEHLMRIGP
                     IQLLKQNA"
     gene            complement(6892..7833)
                     /gene="hemC"
                     /locus_tag="PLW16_09090"
     CDS             complement(6892..7833)
                     /gene="hemC"
                     /locus_tag="PLW16_09090"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPB71103.1"
                     /translation="MAKIEIKIGSRKSELALWQTRYVQDRLNQAGLSASVVTVETQGD
                     RMLDTSIAKIGSKGVFTEALEVELATGAIDIAVHSAKDMPSTLPEGFELIAFSEREQP
                     ADVLVSRNQALTMGDPGRPITIGTSSVRRQALLKRHYPHVRTVDIRGNLQTRIRKMDA
                     GLCDGIIMAYAGMHRMGMDALIVHTFPLDRFVPPVGQGCIAVEAAASLPAEKRALIRA
                     AVNHRASEACLLAERAFLRQLEGGCSIPVFALSALENDEVRLYGGLMSLDGRKFLSGT
                     RSGNRREAAEIGKGLGAHILQAGGAAILAEIRQHLEA"
BASE COUNT         1616 a         2436 c         2574 g         1791 t
ORIGIN      
        1 gaggtgatcg acgcgccctc ccggcgcagg cgctgcaggg cgagctcccg gttccgggtc
       61 gtccgggagg agaccgcgtc ggcgacgacg tggagttcgt agccggcctc gatcaggtcc
      121 agggccgtct ggtacacgca gatgtgggcc tcgatgcccg tgagcaggat ctgcctgcgg
      181 cctgcggccg cgagggcgtc cgtgaaggcg ggaaccgcgc agcagctgaa gctctccttc
      241 cggatggggg cggtcccggt caggagtggg gcgagctccg ggatcgtcgg tcccagtttc
      301 tccggggtct gttccgtgac cacgacgggc aggccgaaga ccttcgcccc ggcgacgatc
      361 cgggccgcgt tcgcgaacag ggactccttt tcgtccatgg cccgggccag attcccctgg
      421 atgtcgatga cgacgaggag cgcccgctct tcggaaagcc gcatcccgtt ttccctcccg
      481 ttttttgctt ctcttaacat gatctccgct tgttttcaac ggacggcgat gatatgtccc
      541 gcaggaaccg gggtcgggtc tgcgcaggcg gcgatttgcg cggccgccga tcgaagcgca
      601 ccggaaacgc caacgaaagg gaggaaagca tgctcggact catcgcgaaa ttgaagatcc
      661 aggaaggccg gatggacgag gccctcgacc tcttcaaggg gctggttgcg gacgtcaaga
      721 cggaggcggg gacgctttcg tacacggtga acaaggatac ggcggacccc aacgtcattg
      781 tggtcgtgga gcgctacaag gacgccgatg cgctgacggc ccattcctcg actccccact
      841 tcgccgcctt ctcgaagcgg atcgcggggc ttctcgacgg ccagatggag atgagcatcc
      901 tggaggaggt ggcgtcgatc acataagcgg gctctgggct gaactttaaa atcattggaa
      961 gggagatttt agacggatgg gaaacgggtt ggtgctttac gaggaacggg accgggtggc
     1021 gatcctgacg atcaatcgcc cccaggcgat gaacgccctg aacacggacg tcaatctgag
     1081 gctcatcgaa ctcctgcttg aggccgagcg ggatccggcg atcaaggtgg cggtgctgac
     1141 gggtgcgggc gggaaggcct tcatcgccgg cggggacatc aaggaaatgc tggggatgga
     1201 tgccatgcag gcccgggggt acgccctgaa tgcaaagcgg gtcatcgata cgatctggaa
     1261 cctggggaag cccgtgatcg ccgcgatcaa cggggtctgc ctcgggggcg gcctcgagta
     1321 cgcgatggcc tgcgatctga gaacggcggc tgagacggcg aagttcgccc tgccggagat
     1381 caacatcggc atcatgccgg gcagcgccgg gacgcagcgg ctgccccgcc tgatcggcat
     1441 ggccaaggcc aaggagttct gcttcaccgg ttcgatgttc gacgcgaaac tggccttgga
     1501 actggggctc ctcaatcacg tctatcccgc cgagtccctg atggaggaaa ccctggcgct
     1561 ggccggaaag atcgtttcga aatccggccc ctcgctgcgg ctgatcaagg cggccatgaa
     1621 ccgggggacg gaaacggacc tggagacggc ctccctgttc gaaatagact gcttcggcct
     1681 gtgcttctcc acgcaggagc ggcaggacgg tatgcgggcc ttcgtggaga agggcaggaa
     1741 gtaacccagc gggaaatttt cgagggatgc gttccgtgcc gatgtgacgt cagcgggaag
     1801 cgtccctcat ccttttcatc tgatcggaga acggtttatg tttagcccgc ccggtcgcct
     1861 gaaatctaaa ttgcataagt caacaacgcc cattttgtca tttgctcact tcagcaggaa
     1921 cacgagggtc cggccgggga ttttctggtc gtcgatttcg tcaccgctca ggatgcgacc
     1981 gtcgggaagg cggtagaacg tcgagggata attccatttg cggccggcaa tccgggaggc
     2041 ggagcgaacg ccttgcacat atccgccgta gatgtagccc gccagcaccc gggttccctt
     2101 gggcggcctc tcgagcagtt tgcggagcga ggcgtttttg ttcagctcgg ccccggtccg
     2161 gatgaggccg ctggggaaaa aatagatggt cgttgagtcg gtgtagagat cacccgccag
     2221 gtcgctcgcc ctggagccgt ccctgccgat ctccaggaac ccttcgaatg actgttcccg
     2281 ctccggaaca tcctcgaaac ggacgcgcgt gccggcagga atcgcttccc attccctgat
     2341 ttcgtctccc cgaaaccttc gtccgtccgg atactcatag atcgttgtcc ggtggtcgta
     2401 tcggtcccgg gcgacgttcc aggcattgat ccccttggcg atgaccctgg tggcttcccg
     2461 ccgttcgtcg ggccgctgcg attcccgggc gccgatcagg gcggcccaca ttctgggggg
     2521 gcgatagaga aagcggtgca gctcgggatc ggcgaccgcg agtcgcctgg ctgcgacatc
     2581 cgggtcccgg gttggtctcg cggaaaggcc cagacgtcgc ctgacgtcgg gatgggcaaa
     2641 gagcatccca cattttttcc tcccgcggtg cctctgggga tgaaagcggt tggggcgccc
     2701 gtaggccacc atggaatggg tgaggacatc cttgtcgcgg atccggtaga tggccttcaa
     2761 ctgtctcagc agctcgcgaa gcgacgcata ctgggcggtg gtgatatcct cgtcgtgata
     2821 gccgacgacc tcgatgccga tggtgaaatc gtcgagggtg ctcttcccct tccacatgct
     2881 tctccccgtg tgggtggcga tccgcctgcg gtcgatgatc cggtaaacct cacccgaggg
     2941 ggcaaccaga tagtgggcct ctccaaagcg gtgcaccttg cggagggaac cttccaaggc
     3001 gccctcagtg gtgtgcagga caatgtggcg tgtggtggtg cgaaagggac gtttttgatt
     3061 caggggactg aagtaattgc tgattgtcag cgctttcgca gccgcctccg aagcccccgc
     3121 gagaagtacc gcgagggtca gaaacaggag gacggcgaac cgtccggcat gctcgatggt
     3181 tgaaacggcg tatctgtaaa acctgaatcc atgcattccg ctgtcgttga acaagacctc
     3241 cccaatatca tccacgcacc ggtcgaattt tcggtgtgga gccggttccc cgcgggtcac
     3301 cgctccagca cgtagctgaa caacaggggc gccacaatgg tggcgtcgct ttcgatcaca
     3361 taacgcgggg tatctaccga taactttccc caggagatct tttcgtttgg caccgccccg
     3421 gaataagaac cgaaactggt gatgctgtcc gaaatctggc agaagtaggc ccagtgcttc
     3481 accttctctt tgaggtcgta attcaacatg ggtaccacgc agatggagaa atcgccggca
     3541 atgccgcctc cgatctggaa aaacccgata cccgccgagg tttccgtgtc aattttctgg
     3601 taccactccg cgagatgcat cagatattct atcccgccgc ggatggaccc cggcttcagt
     3661 tcctttttta tgcacgtggc ggcatagacg ttgcccaggg tagaatcttc ccagccggga
     3721 acgtaaatgg gcagccgctt ttgggccgca gccagaagcc agctgttttc ggggtcaatt
     3781 tgataatgcg ctgtcagttc tcccgagagc agaagatcgt aaaagaattc gtgcggaaac
     3841 ttgagggtgc ctgtcttttc ggcctcccgc cagcgccttt tcagtcggtc ctcgatgtga
     3901 aacatgacgt cctgggggat gccggtgtcc gtgacgcggt tgtagccgcg ctccaggagt
     3961 tcgcgctcct gtgagggact cagattgcga tactccggga tcttgacgta ggtttggttg
     4021 gcgacaaggt tgaagacatc ctcctcgagg ttagccccgg tgcagcaaat cgcatggatt
     4081 ttatcttggc ggatcatctc cgccagacta atccccagtt cggccgtact catggcgccc
     4141 gccatggcca gaaacatctt tccgcccttg ttgatgtgat ccctgtatga cagggcggca
     4201 tctctgagcg tcaaggcgtt gaagtggcgg tagtgcgttt ccataaaacg gctgatactt
     4261 ccctctgcct ggttgcgtga tttgtgctga agcgtcgtgg tgatggacat gatcccgtct
     4321 cctttctcct tgcgcctggc cctgcttttt tggaaaaaaa cggtgtggcc gtgacggtgt
     4381 caggtcgatc cgcgggcaac tgttttaagt ggccgggagt gactgaatta cttggctacg
     4441 ttggaggact ggcaagattt tattttgcgg actataggga aaacaagacg atatgtcaat
     4501 tcaaaagggt ccatccgtca cctccaacgg tgtctaactc tggggaactg cctgtatgcc
     4561 cggaccgttc tggccgcagg cacaacgccg tgcgccttcc tgatgcactt ctccgcccgt
     4621 gcccggcaaa tgggttgatg attcgtacaa gggcttcccc gggcgcggcg gcagagcgga
     4681 aagcatcagg aggcgttggg cgcccgctcc tcgaccaatt cgtaggagcc ttcgccgtcg
     4741 tgggtctccc gccccgttac cggcggattg aaggcgcaga tcagccgcat gtcttccgta
     4801 ccgcccctga ggatatggca atcgtgatca ttgagggcgt agaggacgcc gtccttgacc
     4861 tcgtggacct cgccactggc cagatcctcg atagttccgt tgccggccac gcagtaaacc
     4921 gcctccaggt ggttcttgta ccagaaccgg tgctccgtgc cggccgtgat gatggtctcg
     4981 tgaaaggaaa aacccatgcc gtcttttttc aacagaaggc gacggctcac ccatcccttt
     5041 cccgccacct cccgctccgt gtcgataatc tgccggatgt cgcgaacaat cattacaatt
     5101 cccccgtgag attctctttc ttctttttca ggatctcccc gatagattgc ccgacaatct
     5161 cgatgccgcg caacagggtc tcttcgtcaa tggtcagggc aggcagaaat ttcaccactt
     5221 ggtcgtagct gccggccagt tcgatgatca gaccgttttc gaagcagcgc tccgatacct
     5281 ggctggcgag cccccgttcc ggcagctcga ggccatagat catcccgcgg ccgcgcaccg
     5341 aggcctccag ttccggatag gtctcggcta tcttctttag ctcccgctcc atcaccgtgc
     5401 ccttgtactt cacggcctcg gagaagtcgt tggtgtccca gtagcacagg gcctgggtgg
     5461 aagccacaaa ggccaggttg ttgccgcgga aggtgccggt gtgttcgccc ggtctccact
     5521 ggtccaggtc cggccgcatc aggatgaagg ccatgggcag gccgccgccg atggacttgg
     5581 acaggcaaac gatgtcgggc ttgaggtccg cctcctcgaa gctgaagaaa tcgccgctgc
     5641 gaccgttgcc cacctgaata tcgtcgatga tcatcaggat gtcgaactga tgacagatct
     5701 ccgcgagttc cttgagccat tccggccggg caacgttgat tcccccttcg gcctgaatgg
     5761 tctccaggat caccgcagcc ggcgtgtcca gaccgctgga gttgtcggcc agcatcttgc
     5821 ggaacagatc cagagtgttg aaatccggcc tcagatagcc gtcatagggc ataaagctca
     5881 cattggagcg gctgatatag gcctcgtccc ggtaaaaggc gttgcccgtg atggccaggg
     5941 agcccatggt gagaccatgg aagccgttgg taaagcagac gacgttggag cgggccttga
     6001 ccatgcgggc cagtttcagg gcggtctcca cggcattggt gccggtaggc ccggtaaact
     6061 ggatcttata ctccatgttc cgcggttgca ggatggtgtt gtagaatttt tccaggaagt
     6121 tcttcttcgc cgtggtggcc atgtccagac cgtgcaccac gccgcccaat tggaggtgct
     6181 cgatgagcgc ctgggtgatc tgctcgttgt tgtggccgta attcagggta ccggcgccgg
     6241 cgaaaaagtc aatatactcc ttcccttgct catcgaccat gatggatccc ttggccgtgt
     6301 ggaagatgac gggaaacgac cggatgtacc ccctgacgcg agattccaag ttttcaaaga
     6361 tacgcaaaac atcctcctta tgcattctgt ttcagaagtt ggatcggccc gatccgcatc
     6421 aggtgctccg gtgcatcggt ggaacccatg tccgactggg tgaagaaggg cttgtcggaa
     6481 aaggtcgcgt cggccgccat ttggcgagcc gctgcttgaa acgcccggag agacgcctgg
     6541 ttgtcgaggg caatggtcgc ctcgaggaat gcgggtcgag ccctttcgat cagtcggatc
     6601 agcatctgct tgccgatccc cttgccttgg gccgccctgc tcaccccgat ctgccagacg
     6661 aagagcgtct cgggctgcgc gggcagaaaa agggccgcga tatagccaca aacctgttcc
     6721 ccggaccggg ccaccaaaca ggtgtcggaa aagcaacggg ccatcatcgc gtagtagtag
     6781 gcggaattca cggaaagaac gcccgtgtcc cgggcgatca cccgcatcgc cgggccgtca
     6841 ccgcggcgtg gcttgttcaa ctggatgctg ttatggggct ccatgcgtct gttatgcctc
     6901 cagatgttgt cggatttcgg ccaggatcgc cgcgccgccg gcttgtaaaa tgtgggctcc
     6961 cagtcccttg cctatctctg ccgcctcgcg gcggttgccg ctccgagtcc cggatagaaa
     7021 cttccggcca tcgaggctca tcagcccgcc gtaaaggcgt acctcgtcgt tttcgagagc
     7081 ggacagggca aagacgggaa tggaacagcc cccttccaac tgccgcagaa aggcccgctc
     7141 cgccagcagg cacgcctcgc tcgcccgatg gttgaccgcg gcgcgaatca gcgctcgctt
     7201 ctccgccggc agagaggccg ccgcctccac ggcgatgcag ccctgaccga ccggaggcac
     7261 aaagcgatcc aggggaaagg tgtggacaat cagtgcatcc atgcccatgc ggtgcatccc
     7321 cgcataggcc atgatgatgc cgtcgcagag gcccgcgtcc atcttccgga tgcgggtttg
     7381 gaggttgccg cggatatcca cggttcgcac atggggataa tgacgtttga gcagcgcctg
     7441 gcgtcgcacc gaggaggtcc cgatggtgat ggggcggccg gggtccccca tagtcagcgc
     7501 ttggttgcgg cttaccaaca catcggccgg ctgttcccgc tcgctgaagg cgatcagttc
     7561 aaacccttcc ggtagcgtcg agggcatgtc tttggcgctg tggacggcga tatcgatggc
     7621 gcccgtcgcc aactccacct caagggcctc ggtgaaaacg ccctttgagc cgatcttggc
     7681 aatgctcgta tccaacatcc ggtctccttg ggtctcgacg gtgacgaccg acgcggagag
     7741 gcctgcctgg ttcaagcgat cctggacata gcgggtctgc cagagggcca gttcgctttt
     7801 ccgggatccg attttaatct ctatcttcgc cacaatcctc catgaaaatc gtttcgtacc
     7861 tcttccgaaa acaggatcgt ctggcaattg ctcataagat tacgagcatc gccaaagatt
     7921 ccgaagggac ttggattagt ttaaaacagg atatctagga attcccttga tgttgattca
     7981 tcataacaac ccatgactta tgtgtcaaca taagaatgac gggaaaataa gttaataaaa
     8041 tcaaatagtt agtcctcttt acgctgtcgg ttttgcgtcc gtcatcctta agtgtttgat
     8101 taataacggt tatttagagc atgatcacgc tcaaggcccg tagaatggtg tagagcggcc
     8161 tcgatcttga gtttttgttc ggatctatgc aggacggcct taaatcttta ggcgggttga
     8221 agcttgcaga ttgaacgtga aggatttatc gcaatgctgg ctctctaact tttcatgtgc
     8281 cggagttccc gcagaagatc ccagtctccg gccttgtttt tcctgaagat ccggaagcgg
     8341 gccgttaacg cctccagctc gatcagggcg aaggacgggc cggttcttgt gccggtgtgc
     8401 tggtcgccgg gattgca
//
LOCUS       DAOOLJ010000072         8300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_10097_length_8300_cov_12.283202, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000072 DAOOLJ010000000
VERSION     DAOOLJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_8300_cov_12.283202"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..530)
                     /locus_tag="PLW16_09095"
     CDS             complement(84..530)
                     /locus_tag="PLW16_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB71104.1"
                     /translation="MRVRVSAVFEQAGEILCMRYVYGGKDVYAIPGGGVGKNTLLHDT
                     LIDEWKTELGVKVTVGNILLVGEAPATKRHTQTLHIIFEATDVRGTPKVRPDSTHSLD
                     VAWVPINQLETSPLYPDVGKQLFDYFQSGRTRSIAFTDNCMERGYW"
     gene            763..2145
                     /locus_tag="PLW16_09100"
     CDS             763..2145
                     /locus_tag="PLW16_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HPB71105.1"
                     /translation="MLNRWRGHSSSCSPLRLGTFVVGLILLFFIPIRSVHAEGILDVY
                     RLAIENDPAYKAARFSHEASKETLSQAWAAFQPTISAEGIYTNTDQKILSSDNTVYGQ
                     GSASFPTTEYSVSLIQPLFNMSSLSNLFRAKARVKAADMGMEAARQDLIVRVTKAYLG
                     GLAARDKLALAKTEEASVEMHYELASGKYKMGLTPKTDYLDAKARMAEVRANRITAES
                     VQDDALQALREVTGMPAGDLAGLRVDLPLKNPEPNDIEAWISAAVKQNPTLEAQRQAV
                     EAARQEYKRQLAGHYPMLNFEAGYTYNETEGTLFGGGSEVETTDILLRLNVPIFEGGI
                     VRSRSREAYNLYLAAIQEQERQLRALQKETRAAYYGVADAMERATALREAVEAQELVV
                     QAKQDGYKSGLFTLLAVLDSQRDLTLVRQDYATARYDYVANSLRLKKAVGTLAQSDIE
                     IVQGWLEDGK"
     gene            2176..>8300
                     /locus_tag="PLW16_09105"
     CDS             2176..>8300
                     /locus_tag="PLW16_09105"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HPB71106.1"
                     /translation="MWNLSRNAKSAGKKAEKKTRRKNKRPFQLEALEPRLLLSADLPL
                     PIADVKDLPGVMPAVVEKVTVDAHETVQASDDVQAGNALPPLNVDGVQIQDLTLTPVS
                     DDALEKGRTGLADPSLSPQGDTAADAEIGKAVSGAASDAGSAEGDSGLLSDRRDPREV
                     IIVDPSVTHYTSVVEALKAKSAAHGSLEAGSTAADQEWFIHVLDSAGDGIEQITGILK
                     SYKNLDAVHLLSHGASGEMSLGATALLHQGLQDRADEISAWGSSLKAGGDILLYGCNI
                     AEGAAGVDFVQTLASLTGADVAASTDGTGGTAAGGDWDLEYRTGEVQASPAFTASDAN
                     YPYLMEDVVGTTADDNLTGTGEGDRYVFDDDWGTDTISEPAVASGTDILDFSNVEADL
                     TFTTDADGKISVTDGDNILFPVAGIEKIIGGTGNNTLIGPEGGATWTLTGGDSGMVGT
                     LAFSGISHLQGSANNDTFVVQSNARWSGTIDGGGGTNTLDASPVSDRLLFTIRADGTV
                     GLSTAPDTWASLGIIPAEVLSLLTDGISIESVRNMDKLVGGSGDNTFAFQENAAFAGI
                     LDGGTGSTNTLDYSAYAAAVTVNFVNKTATATDGINNVNRVVGKKSQLTDGSGNSTVT
                     GTGSEATIAYSDDEGVVASLLTSIGLKTIEGTPGDDVLTGNDGINILDGKSGDDLLIG
                     GAGSDIYTFTDGWGADTIQDTSGDLDTLDFTLCTADLTFTIHTDGTVSVTDGTNKISK
                     AANIEVLIDGQGNDRFVFEDGATFEGTIGKPDWLDKLLGNSLIDYGSNTFDFSAYTTP
                     IYVDLGIKIPTLEMILWQTAWQADDGDDSTDETAIVPYFSNIDNVIGGSAGDYIWGSS
                     GANVLRGGPGDDVIVGRRGADTFDGGLGDDILYGGMTEDELITIIATLAGDPAAIADQ
                     LGISPVDVAQRVLNGETIQSIIMDVIDGDDDVVTYADATGPVTVDLSWHPEVAGDIIY
                     DATTSVFDLLEQVASSVANIANYLSLSTSTDPSLDTSDFDELDDPANILPHSDGAAGH
                     DVILFIHHAIGSNYDDTLKGDFLDNTLKGGAGNDTLEGGLGSDTLVGGLGDDILDGGD
                     SWAGDSTLVGDRDIASYIDATDGVRVDLNIVGAQDTRKWDIFYYENDPVGGLVEGREY
                     EVVKVGDNTFQLKSRSGVVIDLVPAPPGGAHSFKYDGGSISFTPSTPGSIDYATSQIK
                     IIGHGFSTDDVITYDAKGNDIIEGLTAGQSYKVVKVDDNTFTLNDMAGAAVALTLAAP
                     RGTHGFDKNGVLKDFSPFAGDVNYADDTFTIAGHGFSDGDIVTYSAGGEKIYTSQGMG
                     RDTLTNMEGLTGSAYDDVLIGTDKHNVIKGEAGNDFLVSGKGADIVTGGAGSDTISYE
                     DFDMGVFVNLWDPIPQIISNDGDTVTLKEIENVIGSAYDDVLIGDFGNNLLDGGYGND
                     WLTGMMGSDTYRFRDGWGSDKAIDDIEAFLDEIDQADVIDTVGDWLAQAGQWLSDAIN
                     TADVLDILEQAQDKSAEKIPDKLDFSLTTADLLVGIHDHGDVSIRYGASEIVEASEIE
                     VIVTGGGSDTFKFDDGVSFSGTLDGGGVNYDKYPDAAPDVNTLDYSAYTTSIVVDLLA
                     PEDGKPGKAQATQGVYNIHNVIGGSADDTLQGSDGNNLLMGGAGNDLIEGRGGDDTLE
                     GGAGDDALIGGEGFDLAAYVTYVNPVVGSTTGITVDLSLGDARQNTVGAGTDVFSGIE
                     GVVGSDYNDVLKGDDADNMLIGGKGDDTIDGGAGNDFISGDAGNDTLTGGAGDDTIEG
                     GVGNDVMDAGEGNDTVSYIYPGSAVTVDLALTASQNTIGAGWDTLRGFENLIGSQGDD
                     TLKGNAEDNILTGGKGNDILHGLDGDDVLLGEEGNDTLYGGLGEDILSGGHGDDIING
                     YISSLDPGYDPAADVDLDIASYAEAESGVTVNLTVVVAQATGGAGSDRLAHIEGLIGS
                     EYADVLTGDAGDNVLLGQAGDDVIEGGAGDDTIYGGEGIDFASYALSTKPVFVDLGLT
                     WEQETGDDTGWDEIAGIEGLIGSAF"
BASE COUNT         1806 a         2429 c         2545 g         1520 t
ORIGIN      
        1 gtcccttttt tattgctgat aacgtcttgc cgaccgattg tggtcgatga aatactggcc
       61 cgccatcttc gggacggacg ccgctaccag tatccccgct ccatgcagtt gtcggtgaag
      121 gcgatagagc gcgtccggcc ggattggaag tagtcgaaaa gctgtttccc cacgtcggga
      181 tagagcgggg atgtttccaa ctgattgatg gggacccagg ccacatcgag ggaatgggtg
      241 ctgtcaggcc ggactttggg cgtcccccgc acatccgtcg cctcgaagat gatgtgcagg
      301 gtctgggtgt ggcgtttggt tgccggggct tcgccgacga ggagaatgtt gccgaccgtc
      361 accttgacgc ccagttcggt tttccattca tcgatcaggg tgtcgtgaag cagcgtattt
      421 ttgcctacgc cgcccccggg gatggcatag acatcctttc cgccgtacac gtaacgcatg
      481 caaagaatct ctccggcctg ttcaaaaacc gcggacactc ggacgcgcat aattcctcct
      541 ttctcccgaa gccaatatac tgcaaaaaac aaagacgtgc aacgatcctg aacccccaaa
      601 aaaaatctat aaaatcattg acaaggggtc ggtcttcagt tacaagaagt caggggttcg
      661 accggaaggt tcatttatga taaatgctcc gtgattccaa ggccttagga tctatcaaca
      721 aacatcagaa aacagttcaa taagagaggg gaaaacctga ccatgctgaa ccgctggaga
      781 ggtcatagtt catcttgttc tcccctgcgt ttggggacgt tcgttgtggg gctgatcctc
      841 ctttttttca ttccaatccg atctgtccat gcagaaggca tcttggacgt ctatcggttg
      901 gcgatcgaaa acgatccggc ttataaggct gcccgcttca gccatgaagc gtcgaaggaa
      961 accttgagtc aggcgtgggc cgccttccaa ccgacgatct cggccgaagg aatatacaca
     1021 aacaccgacc agaagattct cagcagcgac aatacggttt acggccaggg atccgcatcg
     1081 tttccgacca cggaatattc cgtgtcgctg attcagccac ttttcaacat gtcctccctg
     1141 tccaacctct ttcgcgcgaa agcgcgggtc aaggccgcgg atatggggat ggaagcggcc
     1201 cggcaggatc tcatcgttcg tgtcaccaag gcctatctgg ggggtctcgc agcccgtgat
     1261 aagctcgccc tggccaaaac cgaggaagcg tcggtcgaaa tgcactatga gcttgcatcg
     1321 ggaaagtaca aaatgggcct tacccccaaa accgactatc tcgatgcaaa ggcgcgcatg
     1381 gccgaggtcc gcgcaaaccg gatcaccgcg gaaagcgtcc aggacgacgc gcttcaggcg
     1441 ctgcgcgagg tcactgggat gccggcaggc gatctggccg ggctgcgtgt agacctgccc
     1501 ctgaagaacc cggaaccgaa cgatatcgaa gcctggatct ccgcggccgt taagcagaat
     1561 cccaccctcg aggcgcagcg tcaggcggtg gaggcggcgc gtcaggagta caagcgacag
     1621 ctcgcggggc attatccgat gttgaatttc gaggcgggct atacctacaa cgagaccgag
     1681 ggcacccttt tcgggggcgg cagcgaggtg gagacgaccg acatcctctt gcgcttgaac
     1741 gtcccgatct tcgagggggg aatcgtccgg tctcgttcgc gggaggccta caatctctat
     1801 ctggcggcga ttcaggaaca ggaacgccag ctccgggccc tacaaaagga gacccgggcg
     1861 gcctattacg gtgtggccga tgcgatggag cgggcgaccg ctctccgtga agccgtggag
     1921 gcacaggaac tggtcgtgca ggccaagcag gatggctaca aatcgggctt gttcaccctg
     1981 cttgcggttc tggattcaca acgcgacttg acgctggtca gacaggacta tgcgacggcc
     2041 cggtatgatt atgtcgcgaa cagtttgcgg ctgaagaagg ccgtaggcac attggcacaa
     2101 agcgatatcg agatcgtcca agggtggctc gaagacggca agtaacacga atcaccagtc
     2161 ttcacaggag ttttcatgtg gaatttaagc cgcaacgcaa aatccgcggg taagaaagcg
     2221 gaaaagaaaa ctcgacggaa gaacaagcgc ccctttcagc tagaggccct ggagccgaga
     2281 ctccttcttt ccgcggacct tcccctcccg atcgccgacg tcaaggacct tccgggcgtc
     2341 atgccggccg ttgtcgagaa agtgacggtc gacgcacacg agaccgtcca ggcctcggat
     2401 gacgttcagg ccggcaatgc actccccccg ttgaacgtgg acggcgtgca gatccaggac
     2461 ctgacgttga ctccggtctc ggacgatgcc ctggagaagg gccggaccgg gcttgcggac
     2521 ccctccttga gcccccaggg cgacacagcc gcggacgccg agatcggaaa ggccgtctca
     2581 ggtgcggcgt cggatgccgg atccgcggaa ggggattccg gccttctgtc tgaccgacgc
     2641 gacccgcgcg aggtcatcat cgtcgatccc tccgtaaccc actacacctc agtcgtggag
     2701 gccctgaagg cgaagagtgc ggcgcatggc tctctcgagg ccggatcgac ggccgcggac
     2761 caagagtggt tcatccacgt gctcgattcc gccggcgacg gcatcgagca aatcacgggg
     2821 attctcaaat cctacaagaa cctggatgca gttcatctcc tctcgcacgg cgcttccgga
     2881 gagatgagtc tcggggcgac cgcgcttctg catcaggggc tgcaggatcg cgccgatgaa
     2941 atttcggcct gggggagttc cctgaaggcc ggcggtgaca ttctgcttta tggatgcaac
     3001 attgccgaag gagcggccgg cgtcgacttc gttcagacct tggcgtcgct gaccggtgcg
     3061 gacgtggccg cctcgaccga cggcacgggc ggaacggccg cgggggggga ttgggatctg
     3121 gagtaccgca cgggggaggt gcaggcgtct ccggccttca ccgcctcgga tgccaattac
     3181 ccgtatctca tggaggacgt cgtcggcacc accgccgacg ataacctgac cggcaccggg
     3241 gaaggcgacc ggtatgtctt cgacgacgac tggggcacgg acacgatcag cgagcccgcg
     3301 gttgcctcgg gcacggatat cctggacttc tccaatgtcg aagccgacct gaccttcacg
     3361 accgacgcgg acggaaaaat ctccgtgacg gacggcgaca acatcctctt cccggttgcc
     3421 gggatcgaaa agatcatcgg cgggaccggc aacaacaccc tgatcggtcc ggagggaggc
     3481 gcgacctgga cgctgaccgg cggcgacagc gggatggtcg gcacgctggc cttcagcggc
     3541 atcagccatc tccaggggtc cgccaacaac gatacctttg tggtccaaag caacgcccga
     3601 tggtccggga ccatcgacgg cggaggcggg acgaataccc tcgatgcatc acccgtttcc
     3661 gaccgcctcc tcttcacgat ccgtgcggac gggaccgttg gcctcagcac cgcccccgac
     3721 acctgggcgt ccctgggaat tatccctgcg gaggtgctct ccctgctgac ggacgggatc
     3781 tcgattgaga gcgtccggaa catggacaaa ctggtcggcg gaagcggaga caatacgttt
     3841 gccttccagg aaaacgctgc tttcgcgggg atcctcgacg gcgggaccgg gtcaaccaac
     3901 accctggact actcggcgta tgccgccgcc gtgaccgtga acttcgtcaa caaaacggca
     3961 acggccaccg atggcatcaa taatgtgaac cgcgtcgttg gcaaaaagtc ccagttgacg
     4021 gatggaagcg gcaattcgac ggttacaggg accggatcgg aagcgaccat tgcctactcg
     4081 gacgatgaag gggtcgtggc atcgctgctt acctcgatcg gactcaagac catcgagggc
     4141 acgcctgggg acgatgtcct gaccggcaac gatgggatca atatcctcga cggaaaatcg
     4201 ggcgatgacc tcctgatcgg cggcgccggc tcggacatct acacgttcac ggacggctgg
     4261 ggcgccgata ccatccagga tacaagcggt gatcttgata ccctcgactt caccctgtgc
     4321 accgcggatc tgacattcac catccacacc gacggtacgg tgagcgttac cgacggaacc
     4381 aacaagatct ctaaagcggc caacatcgag gtgctgatcg acggccaggg caacgaccgg
     4441 ttcgtctttg aagacggcgc aaccttcgaa ggcaccatcg gcaagccaga ctggctcgac
     4501 aagctcctcg gcaacagcct catcgattac ggctcaaata ccttcgattt ttcggcttac
     4561 accaccccga tttacgtgga tctgggcatc aagatcccca ccctggaaat gattctctgg
     4621 cagaccgcgt ggcaggccga tgacggagac gatagcacgg atgaaaccgc aatcgttccc
     4681 tacttcagca atatcgacaa cgtcatcggc ggttcagccg gcgattatat ctggggcagc
     4741 tcgggagcca atgtcctgag gggcgggccc ggagatgatg tcatcgtcgg acggcggggc
     4801 gccgacacgt ttgacggagg actgggcgac gacatcctgt acggcgggat gaccgaagat
     4861 gagctgataa cgatcatcgc tacgcttgca ggcgacccgg ctgcaatcgc ggaccaattg
     4921 ggtatatcgc ccgttgacgt cgcccagcgc gtcctaaatg gggaaacgat ccagagcatc
     4981 atcatggacg tgatcgacgg cgacgatgac gtggtaacct atgccgatgc gaccggtccg
     5041 gtcaccgtgg acctgagctg gcacccggaa gtcgcggggg acatcatcta cgacgcaaca
     5101 acgtcagtct ttgacctgct tgaacaagtg gcgtcaagtg tcgcgaacat tgccaattac
     5161 ctgtccctta gcacaagcac ggacccgagt ctcgatacgt ctgactttga tgagttggac
     5221 gatccggcca acatcctgcc gcacagcgac ggggctgccg gtcatgacgt gattttgttc
     5281 atccatcacg ccatcggatc caactacgac gataccctga aaggcgattt cttagacaac
     5341 accctcaaag gcggtgcggg taacgacacc cttgaaggcg gcctgggatc ggacaccctc
     5401 gtgggtggct tgggcgatga cattctggac ggtggcgatt cctgggcagg ggacagcacc
     5461 ctggtggggg accgcgatat cgcctcctac atcgacgcga cggacggggt ccgggtcgat
     5521 ctcaatatcg ttggggccca ggacacccgc aaatgggaca tcttctatta tgaaaacgat
     5581 ccggtcggcg gcttggtcga gggccgggaa tacgaggtcg tgaaagtcgg cgacaacacc
     5641 ttccagttga aaagccgaag cggcgttgtg atcgatctgg ttccggcgcc ccccggtggc
     5701 gctcacagct tcaagtatga cggcggctct atatccttca cgccctcgac tcccggctcg
     5761 atcgactacg caaccagcca gatcaaaatc atcggacacg gctttagcac ggatgatgtc
     5821 atcacctatg acgccaaagg caacgacatc atcgagggcc tcaccgcggg ccagagttac
     5881 aaggttgtca aggtcgatga caataccttt acactgaatg acatggcggg agcggccgtt
     5941 gccctgaccc tggccgctcc acggggtacc cacggctttg acaaaaatgg ggttctgaag
     6001 gacttcagcc cgttcgccgg tgatgtgaat tatgccgacg atacctttac catcgccgga
     6061 cacggattca gcgatggcga catcgtcacc tacagcgccg gtggcgaaaa gatctatacc
     6121 agccaaggca tgggccggga caccctgacc aacatggaag gcctcacggg gtccgcctat
     6181 gacgacgtcc tgatcggcac ggacaaacac aacgtcatca agggcgaggc cgggaatgac
     6241 ttcctggtga gcggcaaggg ggcggatatc gtaaccggcg gggccggcag cgataccatc
     6301 tcctacgaag actttgatat gggcgtgttt gtcaacctct gggatcccat accccagatt
     6361 atttccaacg acggcgatac cgtcaccctc aaggagatcg aaaacgtcat cggctcggcc
     6421 tacgacgacg ttctgatcgg cgatttcggg aacaacctgc tcgacggagg ctacggcaac
     6481 gactggctga cgggcatgat gggatccgac acctaccggt tccgggacgg ctgggggagt
     6541 gacaaggcga ttgacgacat cgaggccttc ctggacgaga tcgatcaggc cgatgtgatc
     6601 gataccgtcg gggactggct tgcgcaggcc ggccagtggc tgtcggatgc catcaatacc
     6661 gccgacgtcc tcgacatcct cgaacaggcg caggataaat ccgccgaaaa gatccccgac
     6721 aagctcgatt tctccctgac caccgccgac ctcctggtcg ggatccacga tcacggtgat
     6781 gtgtccatcc gctacggcgc cagcgaaatc gtcgaggcct ccgagatcga ggtgatcgtc
     6841 accgggggcg gcagcgatac cttcaaattc gatgacggcg tatcgttcag cggcaccctt
     6901 gacggcggcg gtgtcaatta cgacaagtac cccgatgccg ctcccgatgt gaacaccctg
     6961 gactactcgg cctacacgac atcgatcgtc gttgacctcc tggctcccga agacggaaag
     7021 cccgggaaag cgcaggcgac gcagggcgtc tataacatcc acaacgtgat tggaggcagt
     7081 gcggacgaca ccctgcaggg gtccgacggc aacaacctgt tgatgggcgg ggccggcaac
     7141 gacctcatcg agggccgtgg cggcgacgac accctcgaag ggggagcggg agacgacgcc
     7201 ctcatcggcg gcgaaggctt cgatctggcc gcctacgtca cgtatgtgaa tccggttgtc
     7261 ggtagcacga caggcatcac cgtcgatctg agtttgggcg acgccaggca gaatacggtc
     7321 ggggccggga cggacgtgtt cagcggcatc gagggggtcg tcggatcgga ttacaatgac
     7381 gtcctcaagg gtgacgatgc ggacaacatg ctcatcggcg gcaaagggga tgacacgatc
     7441 gacggcgggg ccggaaacga tttcatctcc ggtgacgcgg ggaacgatac cctgaccggc
     7501 ggcgccggcg acgacacgat cgagggcggc gtcggcaatg atgtcatgga cgccggtgag
     7561 ggaaacgaca ccgtatcgta catctatccg ggttccgcgg tgacggtgga cctcgccctt
     7621 acggcatcac agaacacgat cggcgccggg tgggacaccc tgcgcggatt tgagaatctt
     7681 atcggctcac agggagatga cacacttaaa ggcaatgccg aagacaacat cctgaccggc
     7741 ggcaagggga acgacatcct gcacggtctg gatggcgatg acgtccttct gggcgaggag
     7801 ggcaatgaca ccctgtacgg gggtctcgga gaggacatcc tctcgggtgg tcatggggat
     7861 gatatcatca atggttatat ctccagcctc gatccggggt atgaccccgc cgcggacgtt
     7921 gatctggaca tcgcctccta tgccgaggcc gagagtggcg tgaccgtcaa cctgaccgtt
     7981 gtagtcgcgc aggccacggg aggcgccggt tcggataggc ttgcccatat cgaggggctg
     8041 atcggttccg aatatgcaga cgtcctgacc ggcgatgccg gcgacaacgt attgctcggc
     8101 caggcggggg acgatgtcat cgaaggcggt gcgggcgatg acacgattta tggcggtgaa
     8161 ggcatcgatt ttgcctcata cgcgctgtcg acgaagcccg tcttcgttga cctgggcctt
     8221 acatgggagc aggagaccgg cgatgacacg ggctgggatg aaatcgccgg tattgaaggc
     8281 ctgatcggat cggccttcaa
//
LOCUS       DAOOLJ010000073         8290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_10115_length_8290_cov_13.437523, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000073 DAOOLJ010000000
VERSION     DAOOLJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10115_length_8290_cov_13.437523"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLW16_09110"
     CDS             complement(<1..717)
                     /locus_tag="PLW16_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPB71107.1"
                     /translation="MKSKEIAPGAVLFNRALTPEHFLLALSLTPSFARPVPGQFVMIR
                     PEGVGPQLLSRPFSLYGYRGRRDGSRIELLYRTIGAGTRQLAALKAGESVEILGPLGR
                     GFTLGPDVDEVILLAGGMGVAPLTFFAERLGRTKAPPRVVCYFGARIRDELIGLDRLS
                     PLCSELHIATEDGSCGERGVITDLFAARMRRHDPRRAAIYACGPNPMLQTLSRILAGT
                     SLRCQVSVEERMACGIGACLC"
     gene            complement(731..1225)
                     /gene="rimI"
                     /locus_tag="PLW16_09115"
     CDS             complement(731..1225)
                     /gene="rimI"
                     /locus_tag="PLW16_09115"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPB71108.1"
                     /translation="METDVIIEAMISEDIPAVLDIDADSFRVPWTPGLFEQELRCDFS
                     RNLVAWIDGGRGRTVIGYIVCWLVAEEMHVQTLAVRKDMRRRGIASRLVAEMMRIARR
                     AQILESSLEVRRSNLAARGLYEKFGFRVKGVRPAYYSDVREDALIMAADVMPLSETCD
                     QRSG"
     gene            complement(1230..1925)
                     /locus_tag="PLW16_09120"
     CDS             complement(1230..1925)
                     /locus_tag="PLW16_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HPB71109.1"
                     /translation="MVFKAIVIAVLGGFICLDRIVLQGMVSRPVVTGPIIGFILGDAY
                     TGLIVGALMELFWIDRSPIGAYVPPNDTLATVVIAAAAILVGQSMGATSRELVALTFL
                     LCLPAGLLGQRLDIWKTARNERRARLALNEVQAGDMDAVSRRHHQSLLQAYLGDVVLI
                     GVILLPATLFLGWIYPFFPLPFMKTLLVVYFLLPVLGVGVALNTIHVRGVIPVFCGLF
                     LTMNVVLELFRFV"
     gene            complement(1942..2439)
                     /locus_tag="PLW16_09125"
     CDS             complement(1942..2439)
                     /locus_tag="PLW16_09125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HPB71110.1"
                     /translation="MNVVLVRVDNRLVHGQIIEAWIPFTKAACLAVIDDDVANDLFRT
                     TVIRMAVPREIEVIVSRVEDFAGQYLPRPGKEPTIVLFSSIDDVKRARACGFPIAKLN
                     IGNIYADECQIKCSPCVLLSGKDVEVLRALLNAGVQIDVRRVPGDKPLDIRDLIEGAT
                     GESVP"
     gene            complement(2449..2859)
                     /locus_tag="PLW16_09130"
     CDS             complement(2449..2859)
                     /locus_tag="PLW16_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71111.1"
                     /translation="MIGVLIVTHGNLGAELIKAAELIKGSLEGIVPVSVDQTKGMEEV
                     KKEITSALKKLDKGKGVLVLTDLFGGTPSNISLSFMKPGKVEVLTGVNLPMLLKIEDK
                     RSDMDLNEFSHYIKDYGRKNISLASEIMNKKVGG"
     gene            complement(2895..4688)
                     /locus_tag="PLW16_09135"
     CDS             complement(2895..4688)
                     /locus_tag="PLW16_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPB71112.1"
                     /translation="MNPQVEPDNLLEFLESTVKKYPNEPAFGTKNASGVYEWVTYSRF
                     AERVDRVRGGLAQLGVSRDDAVGIIANNRLEWAIAAYASYGLCARFVPMYENELPKTW
                     EYIVSDSTVKVLFVSKPEILDKVGEFPRRIPTLKKLVLIEGEGPDSMAGLENVGKANP
                     VASQMPKGDDIAGLIYTSGTTGDPKGVLLSHRNFTSNSIAGLKMYPEIKGPGDYSLSI
                     LPWAHSYGQTAELNAMIYKGGAIGFMENVTTIVNDMSLVRPTWLIAVPRVFNRIYDGL
                     WKKMDEEGGIARKLFVMGVEAAKRKRELAAEGKTCLMTNLKFKLTDKIVFQKIREKMG
                     GRLKGAMTGSAAMNVEIANFFFDVGIPTYDCYGMTETSPAIAMNASYAYRLGSVGRAI
                     EKVRIEIDRSVVEETAKDGEIVVYGPNVMQGYHNKPEETRQVMTPDGGIRTGDRGRLD
                     EDGYLYITGRIKEQFKLENGKFVFPAALEEDICLNPYVANALIYGENRPYTVCLVVPD
                     FFSLEQYAEKHKLPVDMKALVEHKEIQDLIAQEITDMLKGKYGGYEIPKKYYLLAENF
                     SLENGTLTQTMKVKRKVVLERYMDKIEALYR"
     gene            5033..5473
                     /locus_tag="PLW16_09140"
     CDS             5033..5473
                     /locus_tag="PLW16_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB71113.1"
                     /translation="MKTIKRCPRCGFEEATYRNPVPTVDVIVEVEAKGIVLIRRRNPP
                     HGWAIPGGFVDYGESLEAAAVREAREETGLDVTLLGQLHTYSRPDRDPRQHTITTVFM
                     ARAAGQPRPGDDARDVGIFARNRLPQPLAFDHAEILRDYFERRV"
     gene            complement(5475..6365)
                     /gene="metF"
                     /locus_tag="PLW16_09145"
     CDS             complement(5475..6365)
                     /gene="metF"
                     /locus_tag="PLW16_09145"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPB71114.1"
                     /translation="MHITEILNRHKISFSFEFFPPRDARGAEMLFASIADLTLLKPSY
                     VSVTYGAGGSTRNLTHNLVRRIRKETSLTVVSHLTCVGSTREEIARILDTYAADGIEN
                     VMALRGDPPAGQRDFVAERNGFAHADELVAFIRKRFPAFGIGVAGYPEGHAETPNRLK
                     EMDYLKRKVDAGADFICTQLFFENKDFYDFAERCTLAGIQVPIIAGIMPLTSRQGMLR
                     MADLALGMRYPAGLLKAVSRARDDRFVENVGVQWATEQVRDLIDQGVRGIHFYTLNRS
                     KATLRIYESLGVSDSAQLVE"
     gene            complement(6430..6819)
                     /locus_tag="PLW16_09150"
     CDS             complement(6430..6819)
                     /locus_tag="PLW16_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71115.1"
                     /translation="MPKRTSLKTSWRRAAIAVVTLALIVTPGFVAAAPDLNERPMVKR
                     AEEVTTLKNEFVRKVLERAGLSYEADATGFPVRVQYQQTWESIQKIEVVPLTDPTDAD
                     AGVKGHRIFFFTEDDILQIDSPLPIRK"
     gene            complement(6833..7606)
                     /locus_tag="PLW16_09155"
     CDS             complement(6833..7606)
                     /locus_tag="PLW16_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB71116.1"
                     /translation="MKLRGLLPLFGVAAKIVYLTQWRKTMTSPDWDVPSPLSRLGLED
                     ADGIPEGGFGAVAAPAGVGKTALLVQLALHAMLKDEPVLHVSLQDPVQKIGLWYRELF
                     QNLAGHEGLPRAGQVWERILPNRFIMSFQVEGFTVPRLEERISDLTAQNIFHPRLMIV
                     DGLRFQPEIAPTLKDLKRYAEKQGLRVWFTVHVHRRDTDAHGLGPNELNELLGMFAVI
                     LKLDTVGDEIKIRVEKGFPGGEAPAALSLDPTSLLLRAS"
     gene            complement(7606..>8290)
                     /gene="fusA"
                     /locus_tag="PLW16_09160"
     CDS             complement(7606..>8290)
                     /gene="fusA"
                     /locus_tag="PLW16_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB71117.1"
                     /translation="CDVTTGQPRVAYRETITQRADFNYTHKKQTGGAGQFGRIAGYLE
                     PLEDADFEFVNAIVGGAIPTQFIPACEKGFRSCLAKGPRMEFPVTGIRVVIDDGASHA
                     VDSSDMAFQAAARGAFREGYLRARPVIKEPIMKVVVETPTEFQGSVMGLLNQRRGMIL
                     GAQDEGPQCVIEAQTPLAEMFGFSTVLRSATQGKAQFTMEFAHYRQVPQGLAEKIAEE
                     MAAKKKRAA"
BASE COUNT         1608 a         2558 c         2431 g         1693 t
ORIGIN      
        1 gcacaggcag gcgccgatgc cgcaggccat ccgctcctcg acggagacct ggcagcggag
       61 cgacgtcccc gcaaggatgc gggaaagcgt ctgcagcatg ggattcgggc cgcatgcgta
      121 tatcgcggct cggcggggat catggcgtct catcctcgcc gcaaagagat cggtgatcac
      181 gccccgctcc ccgcagctgc cgtcttcggt ggcgatgtgc agttctgaac acagggggga
      241 cagccggtcg agaccgatca gctcgtcgcg gattctggcg ccgaaatagc agacgactcg
      301 cggaggggcc ttggttcgcc cgaggcgctc ggcgaagaag gtaagcggcg ccacccccat
      361 gccgccagcc agcaaaatca cttcatcgac gtccggccca agtgtgaaac cccggccgag
      421 cggtccgaga atctccacgg attcgcccgc cttcagggcg gccagttgcc gggtgccggc
      481 tccgatggtc cggtacagca gttcgatccg gcttccatcg cgtctgccgc gatacccgta
      541 aaggctgaac ggcctggaga ggagctgcgg tccgacccct tcgggacgga tcatgacgaa
      601 ctgcccggga accggccttg cgaaggacgg cgtcagggac agggcaagca gaaagtgctc
      661 tggagtcagg gcccgattga acagcacagc tcccggcgct atctctttcg atttcatccg
      721 cagggcctcc tcatccactc cgctggtcac atgtttcgga aaggggcatc acgtccgccg
      781 ccatgatcag ggcatcttcc ctgacgtcgc tgtagtaggc aggccttacc cctttgacgc
      841 ggaaaccgaa tttctcgtag agtccacgcg cggccagatt cgaacgtctg acctcgaggc
      901 tggactcgag aatttgcgcg cggcgggcga tgcgcatcat ttcggcgacc agccgcgagg
      961 cgatccctcg ccggcgcatg tcctttctga ccgccagagt ttgaacgtgc atttcctccg
     1021 ccaccaacca gcagacgata tagccgatca ccgtccggcc ccggcccccg tcgatccacg
     1081 cgacgagatt gcgtgaaaaa tcgcaccgca attcctgctc gaacaatccc ggtgtccagg
     1141 ggacgcggaa cgagtccgcg tcgatgtcaa gcacggcggg gatatcctcg gaaatcatgg
     1201 cctcgatgat cacatccgtt tccatgcgtt cagacgaacc ggaacaattc caggacaacg
     1261 ttcatggtca gaaacaaccc gcaaaaaacc ggaatgaccc cccggacgtg gatggtattg
     1321 agggcaacgc ccacccccag cacgggcaac agaaagtaga cgaccaggag tgtcttcatg
     1381 aacggcaagg ggaaaaaggg gtaaatccag ccgaggaaga gcgttgccgg caggaggatg
     1441 accccgatca ggacaacatc gccgagatag gcctgaagca gggattgatg atgccggcgc
     1501 gagacggcgt ccatgtcccc cgcctggact tcgttcagcg ccagacgtgc ccgccgctcg
     1561 ttccgggcgg tcttccagat gtccagcctc tgcccgagca ggcccgcggg aaggcagagg
     1621 agaaaggtca gggcgacgag ttcccgggag gtcgcgccca tggattgtcc caccaggatc
     1681 gcggcggccg cgatgaccac ggtcgccagt gtatcgttgg gcgggacgta cgcgccgatg
     1741 ggggaacggt cgatccagaa aagttccatt aaggccccga cgatcagtcc cgtgtaggcg
     1801 tcaccgagga taaaaccgat gatcggaccg gtgaccaccg ggcgagacac catgccttgc
     1861 aggacgattc tgtcaaggca gatgaaccct cccaggacag ctatgacgat cgccttgaag
     1921 accatacctc tagctcttga ttcatggtac ggattcgccc gtcgccccct cgatgaggtc
     1981 gcgtatgtcc agcggcttat ccccggggac ccgacggacg tcgatctgaa cgcccgcatt
     2041 cagcagtgcg cgaagaactt caacatcctt cccgctcaga agcacacagg gagaacattt
     2101 gatctggcat tcgtcggcat aaatattgcc gatattcagc ttcgcgatcg gaaagccgca
     2161 tgcccgggcg cgcttgacat cgtcgatgga gctgaacagg acgatggtcg gctcctttcc
     2221 cggtcgcggc aggtactgtc cggcaaaatc ctccacacgg ctcacgatga cctcgatttc
     2281 cctcggaacc gccatccgga tgaccgtggt ccgaaagagg tcgttcgcca catcgtcatc
     2341 gatgacggca aggcaggccg ccttcgtaaa ggggatccac gcctcgatga tctgaccgtg
     2401 gaccagccga ttatccaccc ggaccagaac gacgttcatg agacctgttt acccgccgac
     2461 tttcttgttc attatctcgc tggcgaggga gatgtttttg cgaccgtaat ccttgatata
     2521 gtgggaaaat tcattcaaat ccatatccga acgcttgtcc tcgatcttga gcagcatggg
     2581 gaggttcacg ccggtcagga cctcgacctt cccgggcttc atgaacgaga gggaaatatt
     2641 cgacggcgtt cccccgaaga gatccgtgag caccagcacg cccttgccct tgtcgagttt
     2701 ctttagagcg gaggtgatct ccttcttgac ctcttccatc cctttcgtct gatccaccga
     2761 tacggggaca atcccctcga ggcttccctt gatcagttct gccgccttga tcagttctgc
     2821 accaagattg ccgtgcgtaa cgataagcac accgatcata tccgctctcc cccttcctga
     2881 atgttgtgcc tttgctaccg gtaaagcgcc tcgatcttgt ccatatatcg ttcgagaacc
     2941 accttccgct tcaccttcat ggtctgcgtc agcgtgccgt tttccagaga gaaattctcc
     3001 gcgagcaggt aatacttttt cggaatttca tagccgccgt acttgccctt cagcatatcg
     3061 gtgatttcct gtgcgatcag gtcttgaatc tccttgtgct cgacgagcgc cttcatgtcc
     3121 accggcagct tgtgcttctc ggcgtactgt tcgagcgaaa agaagtccgg tacgaccagg
     3181 cagacggtgt aggggcggtt ctctccatag atcagggcgt ttgccacgta cgggttgaga
     3241 cagatgtcct cctccaacgc ggcggggaag acgaacttgc cgttttccag cttgaattgt
     3301 tccttgatcc gtccggtaat gtaaagatac ccgtcctcat ccagacggcc gcgatctccc
     3361 gtccggatgc cgccgtcggg cgtcatgacc tgccgggtct cttccggttt gttgtggtac
     3421 ccttgcatga cattcggtcc gtagacgacg atctcgccat cctttgcggt ttcctcgacg
     3481 acggagcggt cgatctcgat cctcactttt tcaatggcgc ggccgaccga ccccagacgg
     3541 taagcgtagg aggcgttcat ggcaatggcc ggtgaggtct ccgtcatgcc gtagcagtcg
     3601 tacgtgggta tcccgacgtc gaagaagaag ttcgcgatct ccacgttcat ggcggcgctg
     3661 cccgtcatgg cacccttcag ccgcccgccc attttctcgc ggatcttctg gaacacgatc
     3721 ttgtcggtca gcttgaactt caggttggtc atgagacagg tcttcccctc ggcggccagt
     3781 tcgcgtttcc gtttcgccgc ttcgacgccc atgacgaaca gtttcctggc gatcccgccc
     3841 tcttcgtcca tcttcttcca gagaccgtcg tagatccggt tgaagacgcg gggaacggcg
     3901 atcagccatg tggggcggac gagggacatg tcgttgacaa tggtggtgac gttttccatg
     3961 aaaccgatcg ccccgccttt gtaaatcatg gcgttgagtt cggccgtctg cccgtaggaa
     4021 tgcgcccagg gaagaatgga gaggctgtag tctcccggcc ccttgatttc gggatacatc
     4081 ttgaggcctg caatggagtt gctggtaaag ttgcggtgcg aaagcaggac accctttgga
     4141 tctcccgtcg ttcctgaggt gtagatcagg ccggcgatat cgtctccctt gggcatctgt
     4201 gaagcgacgg ggttcgcctt tccgacattt tccagcccgg ccatcgaatc gggtccttcg
     4261 ccctcgatca ggaccaattt tttcagtgtc ggaatgcgcc gcggaaactc cccgaccttg
     4321 tccaggatct cgggcttcga cacgaagagg accttgaccg tactgtcact cacgatgtac
     4381 tcccaggttt tcggcaactc attctcgtac atgggcacga accgcgcgca cagcccgtag
     4441 gaggcgtagg ccgcgatggc ccattccagg cggttgttgg caatgatgcc gacggcgtca
     4501 tcccggctga cgccgagctg cgccagcccc ccccgaacgc gatccacgcg ttcggcaaac
     4561 cggctgtatg tgacccattc atagacgccg ctggcgtttt tcgtcccgaa ggccggttca
     4621 ttcggatact ttttaacggt gctttccagg aattccagaa gattgtcagg ttcgacctgc
     4681 gggttcatgt gctcctcctt cacaatggac gttctgtatc gatttcccga acggccaaga
     4741 gattcggcct tcggcgtagc ttgcggcaac ggcggcgcgc tccgtcaggg aggtcgctcg
     4801 agacgggcga cgttcctgcg cctctgtgcc ggcgaagttc gaaagaaacg gatccggcga
     4861 ggccgctacc gcgcctcccg tatcggcagc ggttctttac gcctaatgtg gagaaatgtc
     4921 aaatatattc tttcctttcc ggacagcgac ctcagacgtg aatcgccttg acagaacggg
     4981 aatccccctt taatgttgac tgaatttccg cggaaagaga aaggcagaag ccgtgaaaac
     5041 gatcaagcgc tgcccgcgtt gcgggttcga agaagccacg taccggaacc ccgtccccac
     5101 ggtcgacgtg attgtagagg tcgaggcgaa ggggatcgtc ctgatccgga gaaggaatcc
     5161 cccacatgga tgggccatcc cggggggatt cgtcgattat ggggagtccc tcgaggcggc
     5221 cgcggtccgg gaagcaagag aagaaacggg actggacgtg acccttctgg gccaactgca
     5281 cacctattca cggcccgacc gggacccgcg ccaacatacg atcacgacag tgttcatggc
     5341 ccgcgccgcc ggtcagcctc gacccggcga cgacgcccgg gatgtgggga tctttgcgcg
     5401 gaaccgcctg ccgcaacccc tggctttcga tcacgcggag atcctgcggg attacttcga
     5461 gcgccgcgtg tgactcattc aaccagttgc gcggaatccg acacgccgag cgattcgtaa
     5521 atccggaggg tcgccttcga acggttcagc gtatagaagt ggatgccccg gaccccctga
     5581 tcgatgaggt cccggacctg ctccgtcgcc cattgcacac ccacattctc gacaaaccga
     5641 tcgtcccggg cgcgcgatac cgccttcagc aggccggcgg gatatctcat gccgagcgcc
     5701 agatcggcca tgcggagcat cccctgccgt gaggtcaacg gcatgatacc ggcgatgatg
     5761 gggacctgga tcccggccag ggtgcagcgt tccgcgaagt cgtagaaatc tttgttttcg
     5821 aagaacagct gtgtgcagat gaagtcggct cccgcatcaa cctttctctt cagatagtcc
     5881 atctccttga gtcggttggg ggtttccgcg tgcccctccg gatagccggc aacgccgatg
     5941 ccgaacgcgg ggaaccgttt ccggatgaat gccaccagtt cgtcggcatg tgcgaagccg
     6001 tttctctccg ccacgaagtc ccgctgtccg gccggaggat ctcccctaag ggccatgacg
     6061 ttttcgatcc cgtcggcggc gtaggtatcc aggatccgcg cgatctcctc ccgcgtcgag
     6121 ccgacgcagg tcagatgaga aaccaccgtg agggatgttt ccttccggat acgccgaacg
     6181 aggttatggg tcaggttgcg tgtcgatccc cccgcgccgt acgtcacgct gacataggac
     6241 ggtttgagta acgtcaaatc ggcgatcgag gcaaagagca tctctgcccc tctggcgtcg
     6301 cggggtggga aaaactcgaa actaaagctg attttatgtc ggttcagaat ttcggtgatg
     6361 tgcatgtcca tcccggattt aaatcgcgcc tccccgtccc ggccgtccaa acgtgcgggc
     6421 gtgtgcacct catttcctga tcggcagggg cgaatcgatc tggaggatat cgtcttccgt
     6481 gaagaaaaag atccggtgac ccttgacgcc cgcgtcggcg tcggtcggat ccgtgagagg
     6541 cacgacctcg atcttttgga tgctttccca ggtctgctga tactggacgc gcacgggaaa
     6601 tcccgtcgcg tcggcctcat aggaaagtcc tgcacgctcg aggaccttcc tgacgaactc
     6661 gttcttcagg gtggtgacct cttccgccct tttgaccatg ggccgttcgt tgaggtccgg
     6721 ggccgcggcc acaaaaccgg gagtcacgat caacgcgagg gtcacgacag cgatcgcggc
     6781 ccgtctccac gacgttttca ggctggttcg tttcggcatc gtttctcacc tctcaggacg
     6841 cgcggaggag caggctggtc ggatccaggc tcagtgcggc gggcgcttcc ccgcccggaa
     6901 atcctttctc cacgcgaatc ttgatttcgt ccccaaccgt atccagtttg aggatgacgg
     6961 caaacatccc cagaagttcg ttcagctcgt tggggccgag tccatgggcg tccgtatccc
     7021 ttcgatgaac atgcacggta aaccagacgc gcagaccctg cttttccgca taacgtttca
     7081 aatccttcag ggtgggggcg atttcgggct gaaacctcaa gccgtcgacg atcatgagcc
     7141 ggggatgaaa aatgttctgg gcggtcagat cggagatccg ctcttcgagc cgaggcacgg
     7201 tgaatccttc cacctggaag gacatgatga accggttggg aagaatccgc tcccacacct
     7261 gaccggctcg cgggagccct tcgtgtccag cgaggttttg aaagagctcc cggtaccaga
     7321 gaccgatctt ctggacggga tcttgaaggc tgacgtgaag gacaggctca tccttcaaca
     7381 ttgcatgcag ggcaagctgg acaaggaggg ccgtcttccc gacacccgcg ggggcagcga
     7441 cggcaccgaa gcctccctcc ggaatcccgt cggcatcttc gagcccgagg cggctcaggg
     7501 ggctcgggac atcccagtct gggctggtca tggttttcct ccactgagtc agataaacga
     7561 tcttggcggc cacgccgaac agcggcagaa gtcccctcaa cttcatcagg ccgcgcgttt
     7621 ctttttcgcc gccatctcct cggcgatctt ttccgccaga ccctgcggga cttgccggta
     7681 gtgggcgaat tccatcgtga actgggcctt gccctgtgtt gcggaccgca agaccgttga
     7741 aaagccgaac atctcggcca ggggtgtttg cgcttcaata acgcactgcg ggccctcgtc
     7801 ctgcgcgccg agaatcatcc cgcggcgctg attgagcagc cccatcacgg atccttgaaa
     7861 ttccgtcggc gtctcgacga ccaccttcat gatgggctcc ttgatcaccg ggcgcgcccg
     7921 gaggtaccct tcgcggaagg cgcctcgcgc cgcggcctga aaggccatgt cggaggagtc
     7981 cacggcgtgt gatgccccat cgtcgatgac cacccggatg cccgtcacgg ggaattccat
     8041 gcgcggaccc ttcgccaggc aggagcggaa ccccttctcg caggccggga taaactgtgt
     8101 gggaatggcc ccccccacga tggcgttgac gaactcgaaa tccgcgtcct ccaggggttc
     8161 gagataaccg gcgatccggc cgaactgacc cgcgccgccg gtctgcttct tgtgagtgta
     8221 gttgaagtcc gcacgctgtg tgatcgtttc ccggtaggcc actcggggct ggccggtggt
     8281 cacgtcgcag
//
LOCUS       DAOOLJ010000074         8121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_10393_length_8121_cov_17.294942, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000074 DAOOLJ010000000
VERSION     DAOOLJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10393_length_8121_cov_17.294942"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1582)
                     /locus_tag="PLW16_09165"
     CDS             complement(296..1582)
                     /locus_tag="PLW16_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71118.1"
                     /translation="MMPTTRPQFPLMRKLLEEADLSLEVVVLLIFGAFLLLFGILLAP
                     IHTGIVPYSPDGTYGLFLVLVSFQMIAMGKTPFGDLRRSWAAVAGGMAVAVLGMSLCF
                     IPGFLTGFARWLVGFVLTAGGLSLFLQLLMAREKGRRWMAGPGILKSLAVCSGAVYLL
                     LAALGLITLCPGAVSDYVTAGTLVATGLGLLSLAGIIQQIIRRWPEADQHPTLKGVAV
                     AEDARKTRRGLFGEANLSLSLATLLLMGVLLTLLGLVLIPVNLGWIPFSPDGLQGLLL
                     TLFAIQMMSLGDSPIGHYRRSWLMVSYGIAFVGLGAFSCIAPGVLTSVLRALLAVLNL
                     TAGGLYFARRLRQRIKERKTPPADPLPPIVTRLFRTQAALNIAVIAFGVNMLVPGLLP
                     GFVNAVIIVANGLLLFQLAFLLMILEQEATPRDCGS"
     gene            complement(1610..2524)
                     /locus_tag="PLW16_09170"
     CDS             complement(1610..2524)
                     /locus_tag="PLW16_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPB71119.1"
                     /translation="MTIDDIRKILVVGTGTMGQKIALQCARYGYEVVAYDAVPKSLEN
                     AKARIPAFAADLVARGTMKPEESEAAIARIRYSSDPRDGADADLVSESIFEDPDLKAK
                     VFAQLNAVCKPQTVFTTNTSTLLPSMYAEATGRPDRFAALHFLGVIDHRLVDIMPHPG
                     TAPEIVELLTAFARRIRQVPLVLKKENPGYVSNAIWGAMNSAAIRISLVDKVASVEDV
                     DRAVMIHLGMPCGPFGLNDQIGLDTIWHVMQSNAKLSGDPGAQAFADEFKRDYIDKGW
                     LGAKSGRGFYTYPDPAYLHPGFLSGDPE"
     gene            complement(2545..3414)
                     /locus_tag="PLW16_09175"
     CDS             complement(2545..3414)
                     /locus_tag="PLW16_09175"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPB71120.1"
                     /translation="MKNGSYFIPREQLHAFMNPGAMNNEEGLYIFWETDPAVARRVLP
                     PMLELLDPAHPIAMVYVVNIREPTFAPWYMEGGLSLLCRYGETVGAYFLNLQLSGPGA
                     HMGLCSGRETSGLPKKMCERIVVERKGDWARAFIERHGRRIFSTEVELGDYNDPLLGI
                     MRQDYKPGYQERGACLLFQYECGPAPAGHMIIPQARLLHYDSVIDYTTWEPARIQSIQ
                     MEHSLDDPWAELLVVKLLGAGYSINSNWVAGLSTLAQYDGAEADSLFSYLFSGRWDRS
                     TLVTGRHQIYGQF"
     gene            complement(3451..4269)
                     /locus_tag="PLW16_09180"
     CDS             complement(3451..4269)
                     /locus_tag="PLW16_09180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB71121.1"
                     /translation="MSETKDYYRNKVAVVTGGASGVGLALAETMLGYEARGVILADVN
                     DANLARETARLNTAYPGRALGVHCDVTLEAEVQGMIAQAAEFGGGRIDLLFNNAGAGF
                     AGYFDQLSNAHWEKAFALNFYGALYGIRAVLPVMRAQGGGHIVDVISGIAFSPMALQS
                     MYAATKAALNALTLALRYEFWDENIRLTSTTPGTTATAIWSAGGQEPPAGAQTPEHSA
                     QTILAGVAKNQRLVFGDADDERGARNCFNPDIADGVDDYLLNVARKRRSGEWVV"
     gene            complement(4296..6239)
                     /locus_tag="PLW16_09185"
     CDS             complement(4296..6239)
                     /locus_tag="PLW16_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB71122.1"
                     /translation="MFDRLFSPVRIAGLEIRNRVAMTPMGVGLAAVGGGVSDDIIAFY
                     EARARGGIGLIVSEICRVMDGAGAGEPCQLAARNGADIQGLGRMVDAVRKYGARMFIQ
                     LHHPGRQGHHEVGGEQPVSASAVANPVTGEVPRALTVPEIGKIQQAFVGGARIAQMAG
                     ADGVELHGAHGYLINGFLSPYLNRRDDQYGGSLENRLRFLLEIVAGIRTVCGRGFPLG
                     VRLSAEEFLGDQGNDLAATCQIAAELEKAGVDFLDISCTIPDSPKFTACIEPGTIEQG
                     WKRYMAAEIKKHVKIPVIAVANIKEPEVAEAILAEGCCDLVGVARGHLADPDWCHKAK
                     AGKPETIRKCIGCLVCFDEIEHARHVKCSVNPTTGREREFARPARDGAGRTVAVVGGG
                     PAGVTAALVLQERGFRTVLFDPSPRLGGTLNVADKGVDKEKITRLVDSLIAQVLDSGV
                     ELRLGEEATVEKVQALSPCGVFVACGARPIVPPIPGIDGENVVLAEDVLTGRATVDGD
                     CIVIGSGMTGLETAEVVLRAGHKTTVVDMLPQIGAGAEIIVILDAKQRMAPYNPVYLP
                     GHKLLKINETGVELECVESGRTISVPARTVILAMGVRPNREVVDRFRTAFPDARVVGD
                     AARGGRILEATQDAYGQAFVFEP"
     gene            complement(6276..7787)
                     /locus_tag="PLW16_09190"
     CDS             complement(6276..7787)
                     /locus_tag="PLW16_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HPB71123.1"
                     /translation="MPFTLLLAMVLSMSGLSAVNSFSTVSLSAIIFTQQDAGCLSGTP
                     ENIPPPLDNGTIPAACTDKLVRPNTTGHPAARAVSTPAPSEESPTRPQPDQKGASGPE
                     TKVDENDKPPTIPFDYSGDFTHRPVLTGDWDGIRTKLLQHGIRIDVLFMPILQGNLSG
                     GLVQKPMFYGSLRYGIDVDTATAGLWRGGSCSFKIQTGCGRSANDQTGQGVINQVDWA
                     AVDAGDGDPPTTVTDAILFQKLNGLFSVAGGRITSRDSNVFASDETTQFLNAAFNNNP
                     AYNTTFPRQTLFASLLANPAPWFTLITAVLDAGPPAAEDRPGTDFNRGITLFTLTSFS
                     VKTAELPGHHRLGGTWSNKVKPTLEGLISPDLYPDTVSSDWGLNYDFDQFLMQDRSSP
                     NRGFGVFGRFGLGNPVTNKTSAFYSFGLGGRGMWPGRPADSFGVGYFYDTTSKRVPPE
                     IRTLLRNERGFEAYYNLGLKPWLELTLDLQVVTPPLKEDAQAVVGGVRLLIRL"
BASE COUNT         1489 a         2517 c         2629 g         1486 t
ORIGIN      
        1 ggcccgaaaa aaagttccgc gtcccgtgcc agatcagctg cggcgcgtat ttcataggga
       61 tatggggcat gacacttcct ttcagttgac gaacaaaatg tcactatagt ggatgccggt
      121 cggattttca accgggaagt cggactgggt ccagggcggt ggcgggctta ctccgatcag
      181 cttcaggtcc aggaccctac ggaaatggtc gtccggggcg agaagttgca catagacaga
      241 ggcgatgaaa acctctggca agggcatcaa atgccgtcgg gcacgaatta atacgtcatg
      301 atccgcaatc gcgtggcgtc gcctcttgct ccagtatcat caggaggaag gccagttgaa
      361 agagcagcaa cccgttggcg acgataatca cagcattcac gaagcccggc aggagccctg
      421 gcacgagcat gttgactccg aacgcgatga cggcgatgtt cagcgccgcc tgcgtgcgga
      481 acaaccgggt gacgatgggc gggagaggat ctgccggcgg ggtcttcctc tccttgatcc
      541 tctggcggag ccgcctggcg aagtaaaggc ccccggccgt gaggttcaat acggcgagga
      601 gggcccggag gacgctagtg agcacccccg gcgcgatgca ggagaatgca cccaggccga
      661 cgaaggcgat gccgtaggag accatcagcc acgaccgccg gtaatggccg atcggagagt
      721 ctcccagcga catcatctgg atggcgaaga gagtgagcag gagaccctgc aagccatcgg
      781 gcgagaaggg gatccatccc agattgacgg gtatcaggac caatccaagg agagtcagca
      841 ggactcccat cagaagaagt gtcgccagcg agagagagag gttggcttcc ccgaacagcc
      901 ctctccgggt tttcctcgca tcttctgcaa cggccactcc tttcaacgtc ggatgctgat
      961 ccgcttccgg ccaccgtcgg atgatctgct gaataatccc ggccagggac aggaggccga
     1021 gtccggtggc gacgagggtc ccggcggtca cgtagtccga gacggccccg ggacacaagg
     1081 tgatcagccc cagggcagcc aacagcaggt ataccgcgcc actacagaca gcgagcgatt
     1141 tcaggatccc gggcccggcc atccaccgcc gccccttctc gcgggccatc aacagctgca
     1201 gaaagaggga taacccgcct gctgttagca cgaatccgac cagccatcgg gcgaagcccg
     1261 taaggaaacc cgggatgaaa cagagggaca tgcccaggac cgcaacggcc atgccgccgg
     1321 caaccgcggc ccaggagcgg cgcaggtcgc cgaaaggcgt cttacccatc gcgatcatct
     1381 gaaaggagac gagcaccagg aaaagcccgt aggtgccgtc cgggctatat ggcacgatcc
     1441 ccgtatgtat cggcgccagg aggatgccga acaggagcaa gaacgctccg aagatgagca
     1501 ggacgacgac ttccagggaa aggtcggcct cctccagcag ctttcgcatg agcgggaatt
     1561 gaggacgagt ggtgggcatc atcggctagg gaccctcttg cgggaacgat cactcgggat
     1621 cgccgcttaa aaaaccgggg tggagatagg ccgggtcggg gtaggtgtag aatccccgac
     1681 cggacttggc ccccagccag cccttgtcga tgtagtcccg cttgaactcg tcggcgaagg
     1741 cctgggcgcc tgggtcaccc gacagcttgg cattgctctg catcacgtgc cagatggtgt
     1801 ccagcccgat ctggtcgttc aggccgaacg ggccgcaggg catgccgagg tggatcatga
     1861 ccgcccggtc gacgtcctca accgaggcga ccttgtccac cagcgagatc cggatggccg
     1921 cggagttcat ggcaccccag atggcgttgg acacgtagcc gggattctcc ttcttgagga
     1981 ccagcgggac ctgccggatc cgccgggcga aggccgtcag cagttccacg atctcgggcg
     2041 cggtccccgg atggggcatg atgtccacga gtcgatggtc gatcacgccc aggaaatgca
     2101 acgcggcgaa ccggtccggc cggccggtag cttcggcgta catggacggc aacagcgtgg
     2161 atgtgttggt tgtgaagacc gtttggggtt tgcagaccgc gttgagttgg gcgaagacct
     2221 tcgccttgag gtcgggatcc tcgaagatcg actcgctcac gaggtccgca tccgccccgt
     2281 cccggggatc ggaggagtag cggatccggg cgatggcggc ttcggattcc tcgggtttca
     2341 ttgtcccccg cgccaccagg tcggcagcga aggcgggaat tcttgccttg gcattctcca
     2401 gggacttcgg gacggcgtcg taggcgacca cttcgtagcc gtagcgggcg cactgcaggg
     2461 cgatcttctg gcccatggtt cccgtgccca ccaccaggat cttgcggatg tcgtcgatgg
     2521 tcatgatgcg tcctccggaa gcgttcagaa ctgcccgtag atctggtgac ggccggtgac
     2581 gagcgtggat cggtcccagc gtcccgagaa caggtacgag aagaggctgt cggcctccgc
     2641 cccgtcgtat tgtgcgagag tcgaaagacc cgcgacccaa ttggagttga tgctgtaccc
     2701 ggcgcccagc agcttcacca ccaacaattc cgcccacggg tcgtcgagtg agtgttccat
     2761 ttgaatcgat tggatgcgcg ccggttccca ggtcgtgtaa tcgatgacgc tgtcgtaatg
     2821 gagcaggcgc gcctgcggaa tgatcatgtg gccagccgga gccgggccgc attcgtactg
     2881 gaacagcagg caggcgcccc gttcctggta ccccggcttg tagtcctggc gcatgattcc
     2941 cagaaggggg tcgttgtagt ctcccaactc cacctcggtc gagaatattc gccggccgtg
     3001 ccgctcgatg aaggcgcgcg cccagtcgcc tttgcgctcg accacgatgc gctcgcacat
     3061 cttcttcggt agcccggagg tctcgcgccc gctgcacagg cccatgtgcg cgcccggacc
     3121 gctgagctgg aggttcagga agtaggcgcc gacggtttcg ccgtaccggc agagcaggct
     3181 gaggccgcct tccatgtacc agggcgcgaa ggtgggttcg cggatgttca cgacgtagac
     3241 catggcgatg gggtgggcgg gatccagcag ctccaacatc ggaggcagca cgcgtctcgc
     3301 cacggcggga tcggtctccc agaagatgta caggccctcc tcgttgttca tggcgccggg
     3361 gttcatgaaa gcgtgaagct gttcccgcgg gatgaaataa ctgccgtttt tcatgggatc
     3421 atgacctcca ttcggttttt cggggggaag ctaaacgacc cattcaccgc tgcggcgctt
     3481 tcgggccacg ttcagcaggt aatcgtccac cccgtcggcg atatcgggat tgaagcagtt
     3541 acgggcgccc ctctcatcgt ccgcgtcgcc gaaaaccagc cgctggttct tcgccacgcc
     3601 ggcgaggatc gtctgcgcgg aatgctcggg cgtctgcgct cccgcgggcg gctcttggcc
     3661 gccggcggac cagatcgcgg tagccgtggt gcccggcgtg gtcgaagtca ggcggatgtt
     3721 ttcgtcccag aactcgtagc gcagggcgag ggtcagcgcg ttcagggcgg ccttcgtggc
     3781 ggcgtacatg gactgcaggg ccatcgggct gaacgcgatc ccggagatga cgtccacgat
     3841 gtggccgccg ccctgcgccc gcatcaccgg cagcacggcg cggatgccgt acagcgcccc
     3901 gtagaaattc agcgcgaacg ccttctccca gtgggcgttc gacaactggt cgaaatatcc
     3961 ggcgaaaccg gcgccggcgt tgttgaacag caggtcgatt ctcccgccgc cgaactccgc
     4021 cgcctgggcg atcatcccct gaacttcggc ctccagagtc acgtcgcagt ggacccccaa
     4081 cgcccgtccg ggataggccg tgttcaggcg tgcggtctcc cgggccaggt tggcatcgtt
     4141 gacgtccgcg aggatgactc ccctcgcttc gtagccgagc attgtctccg caagggccag
     4201 cccgacgccc gaggcaccgc cggtcacgac ggcgaccttg ttgcgataat agtctttcgt
     4261 ttcactcatg gttgttgtac tccctgtcaa ttgaattagg gttcaaacac gaacgcctgg
     4321 ccataggcgt cctgcgtcgc ttccaggatc cgtccgccgc gggccgcgtc gccgacgacc
     4381 cgagcgtcag ggaaggcggt ccggaagcgg tccacgactt cccggttggg acggacgccc
     4441 atggccagaa tcacggtgcg cgcagggacg gagatggtcc ggccgctttc cacgcattcg
     4501 agttcgacac cggtctcatt gattttcaac aacttgtgtc cgggcaggta gaccgggttg
     4561 tagggcgcca tccgctgctt ggcgtcgagg atcacgatga tctccgcgcc ggcgccgatc
     4621 tgcggcagca tgtccaccac cgtcgtcttg tgcccggcgc ggaggaccac ctccgccgtc
     4681 tcaaggccgg tcatccccga cccgatcact atgcagtctc cgtcgaccgt ggcccttccc
     4741 gtcaggacgt cttcggcgag gacgacattc tcgccgtcta tccccgggat gggcgggacg
     4801 atcggccggg caccacaggc gacgaacacg ccacaggggg atagggcctg gaccttctcc
     4861 accgtcgcct cctcgccgag gcgcagctcc acgccgctgt ccagcacctg ggcgatcagg
     4921 gagtcgacca gtcgcgtgat cttctccttg tcgacgccct tgtcggctac gttcagcgtg
     4981 ccgccgagac gcggtgacgg gtcgaacagc acagtgcgga atcctcgctc ctgcagtacc
     5041 agggcggccg tgacccctgc gggccctccg cccacaacag ccactgtccg ccccgctccg
     5101 tcgcgggccg ggcgggcgaa ctcccgttcg cggcccgtgg tcggattgac ggagcacttg
     5161 acgtggcggg catgttcgat ctcgtcgaag cagacgaggc agccgatgca cttgcggatg
     5221 gtttccggtt tgccggcctt ggccttgtgg caccagtcgg ggtcggcgag gtgcccgcgc
     5281 gccacgccca ccaggtcgca gcacccttcc gccaggatcg cctccgcgac ttccggctcc
     5341 ttgatgttgg ccacggcgat gaccgggatc ttcacatgtt tcttgatctc cgcggccatg
     5401 tacctcttcc aaccctgttc gatcgtgccc ggctcgatgc aagcggtgaa cttcgggctg
     5461 tcagggatgg tgcagctgat gtcgaggaag tcgacgccgg ccttctccaa ttccgccgcg
     5521 atttggcacg ttgcggcgag gtcgttcccc tggtcgccca gaaactcctc ggccgagagg
     5581 cggacgccaa gcgggaaccc tcttccgcag accgttcgga tgcccgcgac gatctccagc
     5641 agaaaccgca ggcggttctc gagactgccg ccgtactggt cgtcgcgccg gttgagatag
     5701 ggtgacagga agccgttgat cagataacca tgcgcgccgt gcagctcgac gccatcggcc
     5761 ccggccattt gggcgatgcg ggccccgccc acgaaagcct gctggatttt cccgatctcc
     5821 gggaccgtca gggcgcgggg aacttccccc gtgacgggat tggccaccgc cgaggcggag
     5881 acgggctgct cgccgccaac ctcgtggtgg ccctgtcggc cgggatggtg caactggatg
     5941 aacattcggg cgccgtactt gcgcaccgca tccaccatcc ggccgagccc ttggatgtcg
     6001 gccccgtttc tcgccgcaag ctggcagggc tcgcccgcgc cggcgccgtc catgacccgg
     6061 cagatctcgc tgacgatgag cccgatgccg ccccgggccc gcgcctcgta gaaggcgatg
     6121 atgtcgtcgc tgaccccgcc gcccaccgcg gcgaggccca cgcccatggg ggtcatcgcc
     6181 acgcggttcc ggatctccag tccggcgatg cgcaccgggc taaacagtct gtcgaacatt
     6241 gtttttcacc tcgccgtgtg tcgttcgggc ggctctcaaa gccggatcag cagtctgacc
     6301 cctcccacca ccgcctgcgc atcctccttc aagggcggcg tgacgacctg caggtccagg
     6361 gtgagctcga gccagggttt caggcccaga ttgtagtaag cctcgaaccc ccgttcgttg
     6421 cgcagcaagg tcctgatttc cggcgggacc cgcttgctgg tcgtatcata aaaatacccg
     6481 acgccgaaag aatctgccgg acggccgggc cacatgccgc gtcctcccag cccgaaactg
     6541 tagaacgcgc tcgttttgtt cgtgaccgga tttccgaggc cgaatcgccc gaagactccg
     6601 aacccccgat tgggcgaact ccggtcttgc atgagaaact ggtcgaagtc gtaattgagc
     6661 ccccagtcgc tggataccgt atccgggtat agatccggag agatgagtcc ctccagcgtg
     6721 ggcttgacct tgttgctcca ggttccgccg aggcggtggt gtccgggtaa ttcagcggtc
     6781 ttcacggaaa aggaagttaa ggtgaacagg gtgatccccc ggttgaaatc cgtgccgggc
     6841 ctgtcctcgg cggccggcgg accggcatcg agcacggcgg tgatcagcgt gaaccacggg
     6901 gcgggattag ccagcaacga agcgaacagc gtctggcggg ggaaagtggt gttgtacgct
     6961 ggattgttgt tgaacgcggc gttgaggaac tgggttgttt cgtcggatgc gaagacgttg
     7021 gaatcgcgtg aagtgatccg tcccccggcg acggaaaaca acccgttcag tttctgaaag
     7081 agaattgcgt cggtcaccgt ggtgggtgga tccccgtcac cggcatcgac ggcggcccag
     7141 tccacctgat taatcacccc ctggccggtc tggtcgttgg cgcttcggcc acagcccgtc
     7201 tggatcttga agctgcagga accgccgcgc cacaggccgg cggtggctgt gtccacgtcg
     7261 atgccgtagc ggagcgaccc gtagaacatg ggcttctgga ccagacctcc cgacaggttg
     7321 ccctgtaata tcggcataaa caacacatcg atgcggatgc catgctgcag gagtttcgtg
     7381 cggattccat cccaatcacc ggtcagtacg ggccggtggg tgaagtcgcc ggaataatcg
     7441 aacggaatgg tgggaggctt gtcgttctcg tcaaccttgg tttccggtcc ggaggcgccc
     7501 ttctggtccg gctggggtcg ggttggcgac tcttcagatg gtgccggcgt cgaaacagct
     7561 ctggctgccg gatggccggt tgtgttcgga cggacgagct tgtccgtgca tgccgctggg
     7621 atggtgccat tgtcgagggg gggtgggatg ttctccggcg tgccgctcag gcagccggca
     7681 tcctgttgtg tgaaaatgat ggcagagagc gaaaccgtgg agaatgaatt cactgccgac
     7741 agaccggaca tgcttaatac catcgccagg aggagcgtga acggcatgag actctcttcg
     7801 cagttcttcc ggcaagtaga caggctatcc aactaaaaac ggtcaactta tcaggaagat
     7861 agccgggatg gcatggagaa gcaaggtaaa acagagcaga ttcaagtcgg acaccgggat
     7921 cgtcgccggc tcctgagagc aaggggggtt gccatgcgga aggattggat tgattgcctt
     7981 taagagctga ttcatgcttc atctcgttcg agtggagtat ccggcgtaca ggctcgaaga
     8041 tgcttcagaa ctgcttgcgg ctgtccagca gcagggtgac ggggccgtcg ttgaccagtt
     8101 ccacgtccat catggcctgg a
//
LOCUS       DAOOLJ010000075         7965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_10659_length_7965_cov_16.055499, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000075 DAOOLJ010000000
VERSION     DAOOLJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10659_length_7965_cov_16.055499"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLW16_09195"
     CDS             <1..391
                     /locus_tag="PLW16_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB71124.1"
                     /translation="NNDLDLFGEMDTAAKLHKVRLEDPTVMDAQTVLRMATRGGARCL
                     GLNDRIGTLSLGSAADLIIVELKNKPHLTPLYNPWSQLVYAASGSDVATVVINGRIVL
                     QDRRLVTIDLPAVIEDVERIADRIRSS"
     gene            446..2023
                     /locus_tag="PLW16_09200"
     CDS             446..2023
                     /locus_tag="PLW16_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPB71125.1"
                     /translation="MKLPRFETLTTRLTLLGLGLLWLFTGPQAAAAAVQEVTLFPDSA
                     EVVETAKCAVQGSATTQQAIFTLPAQADPETLVTKTAPGAGWRIEDQSWRKINRQDDE
                     GIRVLKAKIAKLKEERGGLQASLQGIDTQIQFWQNQAKAKTKTLAETVNLAAAIGKNS
                     RRVFQEKAQLEPQIEKIDREIALTQEELKRATGSQETAWEVTLLLGGRGPAELSLTYS
                     YVLNGCGWLPLYRIEARPQEQRIFLVWDAEVWQSSGKDWVDTRVNIATLQPPRSLTPP
                     EFRPWIIQPRPLYRPMVRSKAARADDLQLLAEATDERVGVMAAPVPKTKSTFTLWSLG
                     KRTIPAGPKRKFSIREEYWPAAFTYLLRPSVSNQAFVRADVTLAEPRDIPAGSGQFVI
                     DGAVLGKREFAFSGREGEIYFGVDPLVSARAELLQRQSGEKGFIADRQTQTWDWRLEI
                     QNERTIPVRIRLEEPNPQVRDERIRLKTVLDPDTTEKNAGTLIWSFDLAAGAKKSLHT
                     TYQLDAPKDMNLDTGWR"
     gene            complement(2037..4739)
                     /locus_tag="PLW16_09205"
     CDS             complement(2037..4739)
                     /locus_tag="PLW16_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPB71126.1"
                     /translation="MPLVTFYFQLHQPCRLHPDRDKFLWEEKNREIFLKVAEKCYLPT
                     TRLFTELIRAYPRFRITLSMSGIFLEQAEVYQPAVIEALQELLQAGSVHDQVEYLDET
                     YYHSLTSLFSDPQKTEFRAQVSLHREKMRRLFGIFPTSFRNTELMYNNHIADVVADMG
                     YQSILCEKRADMFKDQDPPVSPNAVFRARGSSLIVLPRNRELSDDVAFRFPHRPLTPD
                     DYAGYLARIDGEAVCLGYDYEHIGEHLWKDSGIFDFWRGLPASLEKHESILCMNPSAV
                     AQRFRDADCPLLDIHGLATSSWADVGRDTSGWLGTLTQYALFHEIEAMEGEARRSGTE
                     LLTKWRHLTTSDHIYFLHESTGDDHAVHSYFNPYGGSIAQPTYVLTRKIDELGGALKR
                     FEILKRSERTALLIITPETGRLPEDMDALAQYVSGKSGGQGEVVSALCEGLVERGIDV
                     HLATLNLKKRFQRERGMNEDQWREVRYKIDSKKIHLVSSAVFSGLMSAYSGDPIRTAV
                     EFQRQVVNNVIKSVRARHQGRLLIHSHDWMAGGLITAYAKATGIPVLHTVHNVFTAHL
                     PVEMLGGLDLSQLSEMIFWSEDGGRICVDCQATGIKNATLINFVGERFLREVVEDFFL
                     DRPIVPPNVRNEVKAKYHHDSAISVLNAPASNMYPEHCGVLMRKYGPEDDIIAAKRQN
                     LIAFQNETGLNVNPDAILFYWPSRLDPVQKGVELLEDQAQAFVEANENAQIAIVADGI
                     GSDRTHVDILGRIAYASKGRIAYHSYNEKLSMQGFAAAHDVFGASLYEPCGQIDQVGN
                     LFGTTATNRDTGGYHDKIRELRLKVDGAPQDMGNGFLFRDYDAGGLRYALEKSLAFHR
                     RPPEIREPQLKRIMTEAREQYDLRKMIAQYIRIYEMLNDGRPLV"
     gene            4896..>7965
                     /gene="hrpA"
                     /locus_tag="PLW16_09210"
     CDS             4896..>7965
                     /gene="hrpA"
                     /locus_tag="PLW16_09210"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HPB71127.1"
                     /translation="MQEDQDRSVRADGRARRDSLLERLNYPAGLPIVSSRTEIIRALR
                     THRVVVIAGETGSGKTTQIPKMCLEAGRGMAGLIGCTQPRRIAAVTVAQRLAEELNEP
                     LGRSVGYKIRFEECRGPGQRIKFMTDGVLLAETQRDRLLRRYDTLIVDEAHERSLNID
                     FVLGIVKTLLDRRNDLHLVITSATIDTEKFSLAFDRAPVIEVTGRTYPVEMLYRPPEP
                     GVSEGDEETHVEAAVAAAVDITARHPDGDLLVFMPTEPDIRETCTLLEGRNTGAWAVL
                     PLYARLPASEQRRVFQPARASRKIIVATNVAETSLTIPNIRFVIDTGLARIGQYNPRT
                     RTYSLPIRGISRSSADQRQGRCGRVRNGVCIRLYSEENYLKRAPFTAPEILRANLAAV
                     ILNMMALKLGSIQQFPFIDKPSPKRIRDGIELLLELGALEPADRTDRQETAREHYRLT
                     PRGRSMARLPLDPRLSRMILEAEREGCLDELLVIAAALSIQDPRERPPDKAAQADQSH
                     RIFADPLSDFASLLTLWTHCLDRVGPTGSQGTLKRYCREQFLSYRRMREWKAVRDELA
                     TLSSEAGVEANAPSLPVSGAKSASKKGRAEDPYARIHRAILSGHLSNIAVKKEKNVYT
                     AAHGREAFLFPGSGLYNRRPRWIVAAEMVETSRLFARTCAQIDPAWIEPLAGPLCQTS
                     YSGPHWDPARGEVVADARVTLFGLLIVPARPVSFGPLFPEEAFQIFLRAALVEGRVRP
                     CPPFLRHNLSLRERVGVLEDKVRRRDILVDDDDLVRFYQTRLPVVWSMQSLMKVVRER
                     GGDAFLRLREEDLWRRPPDEEIPQLYPDETRVAADRFRLVYRYAPEAPDDGVTLLIPP
                     EALPVIRTAAIEQGCPGLWQEKILALLKGLPKEARRRLSPLTESARIISLEMEREYEN
                     LFSALSVFVKQRFGLSVPPAVWSAIPLPAHLQMRFAVWDDERGEVAAGRDLALLQREH
                     GREVCTSALDRLQNQWETPPAVNWAFPDLPERIPLLERGRIRGYAYPALEA"
BASE COUNT         1472 a         2569 c         2489 g         1435 t
ORIGIN      
        1 caacaacgac ctcgatctgt tcggagaaat ggacaccgcc gcgaagcttc acaaggtccg
       61 gctcgaagac ccgaccgtca tggacgcgca gacggtcctt cgaatggcca cccgcggagg
      121 cgcgcgctgc ctggggctca acgatcgcat cggcaccttg agtctcgggt ccgcggctga
      181 cctcatcatc gtcgagctga aaaacaaacc ccacctgacg cccctgtaca acccgtggtc
      241 gcaactggtc tatgccgcct caggttcgga cgtcgccact gtcgtcatca atggaaggat
      301 cgttctgcag gaccggaggc ttgtcacgat cgatctcccc gctgtgatcg aggatgtcga
      361 gaggatcgcc gaccgcattc gatcatcctg atcggatcgc ccggcaggga cctcgaacga
      421 cgtcacgaag gaaggaggat tcgccatgaa gctgccccgc tttgaaaccc tgaccacacg
      481 gttgacgctc ctcgggctcg gcctgctttg gctcttcacc ggccctcagg ccgctgctgc
      541 ggccgtccag gaggtcaccc tcttccccga ttcggcggag gtcgtcgaga cggcaaagtg
      601 cgccgtacag ggaagcgcaa ccacccagca ggccattttc accctgcccg ctcaggcgga
      661 cccggaaacc ctcgtgacga aaaccgctcc gggcgccggc tggcggatcg aggaccagtc
      721 ctggcggaaa atcaaccgcc aggacgatga agggatccgg gttttgaagg caaaaatcgc
      781 aaagctcaag gaggagcgcg ggggtctcca agcctccctg cagggcatcg acacccagat
      841 ccagttctgg cagaaccagg ccaaggccaa aacgaaaacg ctcgcggaga cggtaaacct
      901 cgccgccgcc atcggcaaga acagccgccg cgtctttcag gagaaggccc agttggagcc
      961 gcaaatagaa aagatcgacc gggagattgc cctgacccag gaggagctga aacgcgccac
     1021 cgggagtcag gagacggcct gggaggtcac gctgctcctg ggcggacggg gtccggctga
     1081 gctgtcgctg acctattcct acgtcctgaa cggttgtggg tggctccccc tttaccggat
     1141 cgaggcccga ccccaagagc agcggatctt cctcgtctgg gacgccgaag tctggcagag
     1201 ttccggcaag gactgggtcg atacccgggt caacatcgcc accctccaac ctccgcgatc
     1261 cctgacgccc ccggagtttc gaccctggat tattcagccc cggccgctct atcgccccat
     1321 ggtgcggagc aaggccgccc gcgccgatga ccttcagctc ctggcggagg cgaccgacga
     1381 gagggtgggc gtaatggccg cgcccgtgcc gaagacgaaa agcaccttca ccctctggtc
     1441 gctggggaag cgaacgatcc ctgccggacc gaaacggaaa ttcagcatcc gggaggaata
     1501 ctggccggcc gcgttcacct atctcctgcg gccgagcgtc tccaatcagg cgtttgtccg
     1561 ggcggatgtc acgctcgctg aaccccggga catccccgcc ggatcgggtc agttcgtgat
     1621 cgacggggca gttctcggta agcgtgaatt cgccttctcc gggcgggagg gcgagatcta
     1681 cttcggcgtg gatccgcttg tcagcgcccg cgccgaactg ctccagcgcc agtccgggga
     1741 aaaagggttc atcgccgacc ggcagacgca gacatgggac tggcgccttg agattcagaa
     1801 cgaacggacg atcccggtcc ggatccgcct cgaagagccg aacccgcagg tccgtgacga
     1861 acggatccgt ctcaagaccg tgctggatcc ggacacgacg gaaaagaacg ccggaacgct
     1921 gatctggtct ttcgaccttg cggcgggggc gaagaaatcc ctgcacacga cctatcagct
     1981 cgacgccccg aaagacatga acttggatac gggctggcgc tgatcgcctc acccgctcag
     2041 accagcggcc gcccgtcgtt cagcatttca tagatgcgga tgtactgagc gatcatcttc
     2101 cggaggtcgt actgctcgcg cgcctcggtc atgatgcgct tcaactgggg ctcacggatt
     2161 tccggcggcc gccggtgaaa ggcaaggctt ttctccaagg cgtagcggag cccccccgcg
     2221 tcatagtccc ggaagagaaa tccgttcccc atatcctggg gcgccccgtc caccttcagg
     2281 cgcagttccc gaatcttgtc gtggtacccg ccggtgtccc ggttggtggc cgttgtcccg
     2341 aagaggtttc cgacctggtc gatctggccg cagggttcgt acagggaggc gccgaagacg
     2401 tcgtgcgcgg cggcaaaccc ctgcatggac agcttttcgt tgtaggaatg gtaagcgatc
     2461 cggcccttgg aggcataggc aatgcgcccc aggatatcga cgtgcgtccg gtcgctgccg
     2521 atcccgtccg ccacgatggc gatctgcgcg ttttcgttcg cctcaacgaa tgcctgggcc
     2581 tgatcctcca ggagctccac ccccttctga accgggtcca gccgtgaggg ccagtagaaa
     2641 aggatcgcgt ccgggttgac gttcaagccc gtctcgttct gaaacgcgat cagattctga
     2701 cgcttcgccg cgatgatgtc gtcctcgggg ccgtattttc tcatgaggac cccgcaatgc
     2761 tccggataca tgttcgacgc cggcgcgttc aggaccgaga tggccgagtc gtgatgatac
     2821 ttggccttga cctcgttccg gacattcggg ggaacgatgg gccggtcgag gaagaagtcc
     2881 tccacaacct cccgaaggaa ccgctcgccg acgaaattga tgagggtcgc gttcttgatg
     2941 cccgtggcct gacaatcgac acagattctc ccgccgtcct ccgaccagaa aatcatttcc
     3001 gaaagctggc tcaaatcgag gccgcccagc atctcgacgg gcagatgggc cgtaaagacg
     3061 ttgtggacgg tatgcagaac ggggataccc gtcgctttcg cgtaggccgt gatgaggcct
     3121 cccgccatcc agtcgtgact gtgaatcagc agacgtccct gatggcgggc gcgcaccgac
     3181 ttgatcacgt tgttgacgac ctgacgctgg aactccaccg ccgtgcggat cggatcgccc
     3241 gaataggcac tcatcaaccc cgagaaaacc gcggagctga caaggtgaat cttcttcgaa
     3301 tcgatcttgt agcgcacctc ccgccattgg tcctcgttca tgccgcgctc acgctgaaag
     3361 cgctttttca ggttcagggt cgcgagatgg acgtcgatgc cccgctccac caggccctcg
     3421 cacagggcgg agacaacctc gccctggccg ccgctctttc cggaaacgta ctgggccagg
     3481 gcgtccatgt cttcgggcag ccgacccgtt tccggggtaa tgatgaggag cgccgtccgc
     3541 tccgagcgct tcaggatctc aaaccgcttc agggcgccgc cgagttcgtc aatcttgcgc
     3601 gtcagaacat aggtcggctg cgcgatcgat ccgccgtagg ggttgaagta agaatggacg
     3661 gcgtgatcgt cgccggtgga ctcatgcaaa aaatagatgt gatcggaagt cgtcaggtgc
     3721 cgccatttcg tgagcagctc cgttcccgac cggcgcgctt ccccctccat cgcctcgatt
     3781 tcgtgaaaca gggcatattg ggtcagggtt cccagccagc cggaggtgtc gcgtccgaca
     3841 tccgcccacg aggaggtggc cagtccgtga atatcgagta ggggacaatc ggcgtcgcgg
     3901 aaccgctgtg cgaccgccga cgggttcata cagaggatgc tttcgtgctt ctcgaggctg
     3961 gccgggagtc cgcgccagaa atcgaagatg cctgaatcct tccagagatg ctcgccgatg
     4021 tgttcataat catacccgag gcagacggct tccccgtcaa tccgcgccag gtagccggcg
     4081 tagtcgtccg gggtcagcgg acggtgggga aaacgaaagg ccacgtcatc gctgagttcg
     4141 cgattgcgag gcaggacgat cagggaagat ccccgggcgc ggaagacggc gttcggggaa
     4201 acgggtggat cctggtcctt gaacatgtcg gcccgctttt cacacaggat cgactggtaa
     4261 cccatgtcgg cgacgacgtc ggcgatgtgg ttgttgtaca tcaattcggt gttccgaaag
     4321 gaggtcggaa aaatcccgaa gagccggcgc atcttttcgc gatgcaggga gacctgggcc
     4381 ctgaattccg ttttctgtgg atctgaaaag aggctggtaa gggagtggta gtaggtctcg
     4441 tccaggtatt cgacctggtc atgcacgctg ccggcctgca gaagctcctg gagggcctcg
     4501 atgacggcag gctggtacac ttccgcctgt tcgaggaaga tccccgacat gctcagggtg
     4561 atccgaaagc gggggtaggc ccggatcagc tccgtgaaga ggcgcgtggt cggcaggtaa
     4621 cacttctcgg ccactttgag aaagatctcc cggttctttt cctcccagag aaacttatcc
     4681 cgatccggat ggagacggca gggctggtgc aactggaagt aaaaggtgac caacggcatg
     4741 ctttgccctc cttgtaaccg cattccttat cattatggtg gtttatacga tccggcaccc
     4801 tgaatgtcaa ccggttattc cgtctctgcc gctgtgcgcg attgacaggc gggcgtcctt
     4861 tgtgaaatag ctgccccgcg agggaggtgg catcgatgca ggaagatcaa gacagatccg
     4921 ttcgggcgga cgggcgggcc cggcgggact ctctcctcga gcggctgaac tatcccgcgg
     4981 ggcttcccat cgtttcgtcg cggacggaga ttattcgggc cctcaggacg caccgcgtcg
     5041 tcgtgatcgc cggtgaaacc ggatccggca agaccaccca gattcccaag atgtgtctgg
     5101 aggcgggccg gggcatggcc gggctcatcg gatgcacgca gccgcgacgg attgcggcgg
     5161 taacggtcgc gcagcggctg gccgaggagc tgaacgaacc cctcggccgc tcggtcggat
     5221 acaagatccg cttcgaggag tgccgcggtc ccgggcaacg gatcaagttc atgacggatg
     5281 gggttctcct ggcggaaacg cagcgcgatc gcctgctccg ccgttacgac accctgatcg
     5341 tcgacgaggc gcacgagcga agcctaaaca tcgatttcgt ccttgggatc gtcaagacgc
     5401 tcctcgaccg ccggaacgat ctccatcttg tgatcacctc ggcgaccatc gataccgaga
     5461 aattctcgct cgccttcgac cgggcgcctg tgatcgaggt gacgggccgg acctatccgg
     5521 tggagatgct ttaccgcccc ccggaacccg gcgtatcgga gggtgacgaa gaaacccacg
     5581 tcgaagccgc cgtggcggcg gccgtagaca tcacggcgcg gcatccggac ggcgacctcc
     5641 tggtcttcat gccgacggag ccggacatcc gggagacttg caccctgctc gagggccgca
     5701 acaccggggc gtgggccgtt ttgcccctgt acgcccggct ccccgcctcc gaacagcggc
     5761 gcgtctttca gccggcccgc gcgtcgcgca aaatcatcgt cgccacgaac gtggccgaaa
     5821 cctcgctcac tatcccgaac atccggttcg tcatcgacac cggcctcgcg cggatcggcc
     5881 agtacaaccc gcggacgcga acctacagcc tgcccatccg gggcatttcg cgtagcagcg
     5941 ccgatcagcg tcaaggtcgt tgcggacggg tgcggaacgg cgtctgcatc cgactgtaca
     6001 gcgaggagaa ctatctcaag cgtgcgccct tcacggcgcc tgagatcctc cgggcgaatc
     6061 tggccgcggt gatcctgaac atgatggcgc tgaagctggg gtcgatccag cagttcccat
     6121 ttatcgacaa gcccagcccg aagcgcattc gggacggcat cgagcttctg ctggaactgg
     6181 gcgcgctgga gcccgcggac cgcaccgaca ggcaggaaac ggcccgggag cactaccgcc
     6241 tgacgccccg cgggcgatcc atggcgcggc tgcccctcga tcctcgcctg tcccgcatga
     6301 ttctcgaagc cgagcgcgag gggtgcctcg acgagcttct cgtcatcgcc gctgcgctca
     6361 gcatccaaga cccccgcgag cgtccgccgg acaaggccgc acaggcggac cagtcgcacc
     6421 ggatcttcgc ggatcccctc tccgatttcg cttcgctgct gaccctctgg acccactgcc
     6481 tggaccgggt cgggccgacg ggctcccagg gcaccctgaa gcgctactgt cgggagcagt
     6541 tcctatccta ccgtcggatg cgagagtgga aggcggtccg cgacgagctc gcgaccctgt
     6601 cctcggaggc cggagtcgag gcgaacgccc cgtcgcttcc cgtcagcggg gcgaagtcgg
     6661 catcgaaaaa gggccgcgcc gaggatccct acgcccgtat ccaccgggcc atcctgagcg
     6721 gtcacctgtc caacatcgcc gtcaagaagg agaagaacgt ctacaccgcg gcgcacggtc
     6781 gcgaggcgtt cctgtttccc ggctcgggcc tctacaaccg gcgcccccgt tggatcgtcg
     6841 ccgcggagat ggtggaaacc tctcgtctgt tcgcccgcac ctgcgcacag atcgatcccg
     6901 cctggatcga gcccctggcc ggccccctct gccaaaccag ctacagcggc ccccattggg
     6961 atcccgcgcg aggggaggtt gtggccgacg cccgtgtcac gctcttcggc ctcctgatcg
     7021 ttccggcacg tccggtttcc ttcgggcccc tgtttccgga ggaggcgttc cagattttcc
     7081 tgcgagcggc ccttgtcgag gggcgggtcc ggccctgccc accgtttttg cgccacaacc
     7141 tttcgctcag ggagcgcgtc ggcgttctcg aagataaggt ccgccgtcgt gacatcctcg
     7201 tggatgatga cgatctggtc cgcttttacc agacgcgtct ccccgtcgtg tggagcatgc
     7261 agagtttgat gaaagtcgtt cgggaacgcg gcggagacgc cttcctgcgg ctgcgcgagg
     7321 aggatctctg gcgtcgtcct ccggatgaag agattcccca gctctatccg gacgagacca
     7381 gggtcgcggc ggaccgcttt cggctggtgt accgttacgc cccggaggca ccggacgacg
     7441 gcgtgaccct cctgattccg ccggaggcct tgcccgttat ccggacggcc gcgatcgaac
     7501 agggctgccc cggtctctgg caggaaaaga tcctggccct gctgaaaggc ctcccgaagg
     7561 aggcccgcag gcggctctcg cccctgacgg agagcgcccg gatcatcagt ttggaaatgg
     7621 agcgcgagta cgaaaacctg ttttctgcgc tatccgtctt cgtcaagcaa cgatttggac
     7681 tgagtgtgcc cccggcggtc tggtccgcga tcccgctgcc cgcgcacctg caaatgcgct
     7741 tcgccgtctg ggatgacgag cgcggggagg ttgccgccgg tcgggacctg gcgctccttc
     7801 agcgtgagca tggccgcgag gtctgcacct ctgcgctcga caggcttcaa aaccaatggg
     7861 agacgccgcc tgcggtgaac tgggcgtttc cggaccttcc tgaacgcatc cccctgctcg
     7921 aacgcggccg catccggggg tatgcctacc cggccctcga ggccg
//
LOCUS       DAOOLJ010000076         7863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_10859_length_7863_cov_18.098745, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000076 DAOOLJ010000000
VERSION     DAOOLJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10859_length_7863_cov_18.098745"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLW16_09215"
     CDS             complement(<1..419)
                     /locus_tag="PLW16_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPB71128.1"
                     /translation="MIRFDILSVFPGMFASPLAESLIGKARDKGLVDIRFHDIRDYAT
                     DRHRMTDDAPYGGGGGMVMKVEPIDRALEAILPDRSEGLVILMTPQGEPFSQRRAEEL
                     ASHPRLVLLCGHYEGVDERVREHLVDRELSIGDYVLTG"
     gene            complement(429..932)
                     /gene="rimM"
                     /locus_tag="PLW16_09220"
     CDS             complement(429..932)
                     /gene="rimM"
                     /locus_tag="PLW16_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPB71129.1"
                     /translation="MRFIEIGRIVKAHGVQGRMKAVHFLDDEGLLSSLDEIFIAVGPE
                     GAARSYRIRALESRGGALFLDLEGVADRDAAGALAGQFLLLPEDQAPPLAPDEYYWRD
                     LLDMTVRTEAGEELGRITAIFPTGSNDVYVCTGPDREILLPAIAEVVREVDRAGKVMV
                     VRLLEGL"
     gene            complement(946..1176)
                     /locus_tag="PLW16_09225"
     CDS             complement(946..1176)
                     /locus_tag="PLW16_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPB71130.1"
                     /translation="MKELIRYMAQALVDQPDLVQVSEVVGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAMRTILSAASTKLRKRTVLEIIE"
     gene            complement(1279..1530)
                     /gene="rpsP"
                     /locus_tag="PLW16_09230"
     CDS             complement(1279..1530)
                     /gene="rpsP"
                     /locus_tag="PLW16_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPB71131.1"
                     /translation="MATKIRLTRIGAKGKPFYRIVVANSESPRDGKFLEIVGTYDPNQ
                     EPAAITLAEGRVAEWLAKGAKPTLTVSQLLQKKGIAAKV"
     gene            complement(1608..2942)
                     /gene="ffh"
                     /locus_tag="PLW16_09235"
     CDS             complement(1608..2942)
                     /gene="ffh"
                     /locus_tag="PLW16_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPB71132.1"
                     /translation="MFESLSEKLDGIFKKLKGRGRLDEENIQSALKEIRMALLEADVN
                     FKVVKDFIEEVRARAVGQEVVDSITPGQQVVKIVHDRLIELMGGGTSQLRFGNRIPAP
                     IMLVGLQGCGKTTTAVKLARLLAAQGKRTYLVSADVYRPAAMEQLRVMGEKIGAGIFA
                     AGEERDPVRICVQAVEEARRQGYEAVIVDTAGRLHIDAAMMDELKRIREIVTPSEILF
                     VADAMTGQEAVNVAKKFDELLGIDGVIMTKMDGDARGGAALSLKAVIGKPIKFVGVGE
                     KIDALEVFHPDRMASRILGMGDIVTLVEKAQATVDEKEARELERKIRKNEFTLEDFRD
                     QLRQIKKMGSLTDILGMIPGLSKVKGLKDVTPDERELVRVGAIIDSMTRAERLNYLLL
                     DGSRRKRIARGSGTSVQDVNRLIKNYIEMRKMMKRMTGKGGLKALRRGNFPF"
     gene            complement(3027..3788)
                     /locus_tag="PLW16_09240"
     CDS             complement(3027..3788)
                     /locus_tag="PLW16_09240"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPB71133.1"
                     /translation="MVAKNRKQFEVKLGKTGISLLVFGMAAGLFATFLFGVTVGKNID
                     TYPAAIAGYLPGVLGEWMGWKAPAAPVVLGEAKKETAPPEEDEFDLTFYDTLSKKKEG
                     DIILQRPVAPRPSAKDAPSAPSASLPAARLPATPTAKAPATAGQTQPPAAAAVPGAGK
                     PAPAGLAEKPVAGKEKFLVQLVSYQEKAKAEQLNKKVAALGYRPRVEAVEVPGKGTWY
                     RVVLSGFDSVESGKKTADAVSAKIPGVKGVVRREK"
     gene            complement(3866..5530)
                     /gene="argS"
                     /locus_tag="PLW16_09245"
     CDS             complement(3866..5530)
                     /gene="argS"
                     /locus_tag="PLW16_09245"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPB71134.1"
                     /translation="MKKRLERMVGEALAACMEKGTLAAAELPAVEIELTKDAAHGDYA
                     TNAAMMLASRLKMNPRRIAEALIAELADPGGVVARTEIAGPGFINFFIREGFWATLLE
                     AVEREGTGYGRLDFGAGKRIQVEFVSANPTGPLHIGHARGAVVGDAVAAVLAACGYAV
                     EREYYINDAGNQMHNLGRSVLARCRELDGGDAPFPENGYQGEYIRELAREVLAREGRR
                     CLDEAEGEAIPRLTDHAGGVILDGIKDDLKAFGIVFDRYFSERTLYENDGVARLLAAL
                     EAQGFIYREDGTLWFRTTAFGDEKDRVVIRQNGEPTYFAADIAYHKNKFERGFDTVID
                     VWGADHHGYIPRMHAGVQALGYPKEALQVILVQLVNLLRDGKPVAMSTRSGEFVTLRQ
                     VVDEVGRDAARYNFLMRRSDSHLDFDLEVAKRQSNDNPVYYVQYAHARICSILKTAAE
                     KGVAVPAYGQAALERLTLPEEAALIKTLIRLPEVIEGAARNLEPHRLTFYLNELAGLF
                     HSYYNKNKVVSEDGDLTGARLFLVRAVQTVLANVLGLLGVSAPERM"
     gene            complement(5704..5877)
                     /locus_tag="PLW16_09250"
     CDS             complement(5704..5877)
                     /locus_tag="PLW16_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71135.1"
                     /translation="MAMDRRYCAICAWRGTCQKKFSVSTDATGQVHCADYSRDLAIKD
                     KDIEEAVKKEREG"
     gene            6097..6690
                     /locus_tag="PLW16_09255"
     CDS             6097..6690
                     /locus_tag="PLW16_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71136.1"
                     /translation="MRKYLLWMSVVISVLFLPVVTDAACLIRLESGGRIWTPHCWEEG
                     GELKFCVGEGVAGVEKNMVRHIEKTAMTSGVVYEAPSPARAPADTAEKAEKSPSSPAN
                     VESGAALPKPPAKQVDVKAYQDRMAKLKTDLNRTLARMKKATANNDPGDKEEATAENR
                     RISDEMYRLTDELKEGNNGVLPADWWEGIGKEEPAEP"
     gene            complement(6720..7808)
                     /locus_tag="PLW16_09260"
     CDS             complement(6720..7808)
                     /locus_tag="PLW16_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HPB71137.1"
                     /translation="MAQLIEPRKEVRHSDERGMVLVVVLLLTAVLVLLGSTAVIMSTT
                     DMKISANYKAGTQAFYEAEAGVEQVIYYLRNNPVVYPTAGSPTTVTVTCPADFSFNPT
                     VTLHYVAPYRYRFQMTGIGTNNTSKTIEARIRKIPPSTTSADGAVAMYGGGPAVDFKK
                     GGGGGYAVDGHDYPVPADPNCNGSACDTTADSSLPAKPGLYTVMTPTVTGNVSAHLGG
                     SPTQTLGPSRETEYNNFVNNVLTNNLYQTTLGTRDIPAITVIPNGATLNGTGNGAGII
                     IVDNGGELNIVGNFEFEGLVILRGTGRVFGAGTGNIFGSMITIGHESKLIDLTGSINL
                     FYSSSALSNLQNINSITTLQRTAWRDVF"
BASE COUNT         1294 a         2632 c         2426 g         1511 t
ORIGIN      
        1 cccgtcagca cgtagtcgcc gatggagagc tcccggtcga ccaggtgctc gcgcacccgt
       61 tcgtccaccc cttcgtagtg accacagaga aggaccaggc gcgggtgaga cgccagttcc
      121 tccgcccggc gctgggagaa gggctcgccc tgcggggtca tcaggatgac caggccctcc
      181 gagcggtccg gcaggatcgc ctcgagcgcc cgatcgatgg gttccacctt catgaccatc
      241 ccgccgcccc cgccgtacgg ggcgtcgtcg gtcatgcggt gccggtccgt cgcatagtcc
      301 cggatatcgt ggaaacggat gtcgacgagc cccttgtccc gcgccttccc gatcaggctt
      361 tcggccaggg gcgaggcgaa catcccggga aagaccgaga ggatgtcgaa gcggatcatg
      421 tccccctcct acagcccttc cagcaggcga accaccatga ccttcccggc gcgatcgacc
      481 tcgcggacaa cctcggcgat ggccggcagg aggatctccc ggtccggccc ggtgcagacg
      541 tacacgtcgt tgctccccgt ggggaaaatg gcggtgatgc ggcccagctc ctcgcccgcc
      601 tccgtccgga cggtcatgtc cagcaggtct cgccagtagt attcgtcggg agcgagcggc
      661 ggcgcctgat cttccggcag cagaaggaat tgtcccgcca gcgcgccggc ggcgtcgcgg
      721 tccgccaccc cctcgagatc gaggaataac gccccgcccc gtgattcgag cgcgcggatc
      781 cggtagctcc gggcggcccc ttccggcccg acagcgatga aaatctcatc gagggacgaa
      841 agcagcccct catcgtcgag gaaatggacg gccttcatgc gtccctgtac gccgtgggcc
      901 ttcacgatcc ggcctatttc aatgaaccgc atctctttat tcgatttatt cgataatttc
      961 gagcaccgtc cgcttgcgca atttcgtcga ggcggcgctc aggatggtcc gcatggcctt
     1021 ggcggtccgg ccctgcttgc cgatgacctt gcccaggtcc tccttcgcta ccctgagttc
     1081 gatgacggat gtctgctcgc cgacaacctc ggaaacttga accagatcag gttgatccac
     1141 cagcgcctga gccatgtatc tgatcaattc cttcatcgcc tccacctcca cggattgttg
     1201 acgttcatcc actcgctgtc gacgcctgcg ttcatcgttc gcggaaacgg actcggctcc
     1261 gggcgcactc cgggaaggct acaccttcgc ggcgatcccc tttttctgca gcagctggga
     1321 gaccgtcagg gtcggcttcg cccccttggc cagccattcg gcgacgcggc cttccgcgag
     1381 cgtaatggcg gccggttcct gattggggtc gtacgtcccg acgatctcca gaaacttccc
     1441 gtcccggggg gattcggaat tcgccacgac gatgcgatag aagggtttcc ccttggcgcc
     1501 gatccgcgtt aatctgattt ttgttgccat ccgtctctgt cctaacctcc tgaagtgttc
     1561 tatggcgatt caaaacgaac gcgctatcta acacactttt tccggaatta aaaggggaaa
     1621 ttaccacgcc ggagggcctt cagccccccc ttccccgtca tccgcttcat catcttgcgc
     1681 atctcgatgt agttcttgat caggcggttg acgtcctgga cggacgtgcc gctgccccgg
     1741 gcgatgcgct tgcgccggga gccgtcgagg agcaggtagt tgaggcgctc ggcgcgggtc
     1801 atggagtcga tgatggcccc cacgcggacc agttcccgct cgtcgggggt cacgtccttc
     1861 aggcccttga ccttgctcag accggggatc atcccgagga tgtccgtgag cgaccccatc
     1921 ttcttgatct gccggagctg gtcgcggaag tcctccaggg tgaactcgtt tttgcggatt
     1981 ttccgttcga gctcgcgggc ctccttttcg tccaccgtcg cctgggcctt ctccaccagg
     2041 gtgacgatgt cccccatccc caggatgcgc gaggccatgc ggtccgggtg gaagacctcg
     2101 agggcgtcga tcttctcgcc cacgccgacg aacttgatgg gcttgccgat gacggccttg
     2161 agcgacagcg ccgcgccgcc ccgggcgtcg ccgtccatct tcgtcatgat gacgccgtcg
     2221 atgccgagca gttcgtcgaa cttcttcgcg acgttgacgg cttcctgacc cgtcatggcg
     2281 tcggcgacga agaggatctc cgagggggtg acgatctccc ggatccgctt cagttcgtcc
     2341 atcatcgcgg cgtcgatgtg gaggcgcccc gccgtatcga cgatgaccgc ctcgtagccc
     2401 tggcggcggg cctcctcgac ggcctgcacg cagattctca ccgggtcgcg ttcttctccg
     2461 gcggcgaaga tcccggcgcc gatcttctct cccatcaccc ggagctgctc catggcggcg
     2521 ggccggtaca cgtccgcgga gaccaggtag gtgcgcttgc cctgcgcggc gagcaggcgc
     2581 gccaacttca cggccgtcgt ggtcttgccg cagccctgga ggccgacgag catgatcggg
     2641 gcgggaatcc ggttgccgaa gcggagctgg ctcgtaccgc cgcccatgag ctcgatcaat
     2701 cggtcgtgga cgatcttgac gacctgctgg ccgggggtga tgctgtccac gacctcctgg
     2761 ccgacggccc gcgcacggac ctcctcgatg aaatccttga cgaccttgaa gttgacatcc
     2821 gcttcgagca gggccatgcg gatctccttc agggcggact ggatgttctc ctcgtccagc
     2881 cgtccgcggc ccttgagctt cttgaagatg ccgtccagtt tttccgaaag actctcgaac
     2941 ataccacccc gtcaattctc gttgtcccgc ccccggcggc gaacccggta ttcccgagcc
     3001 ctcccgggga cggcgacccg cgtctcttat ttttcccggc ggacgacgcc cttgacgccg
     3061 gggatcttgg ccgacacggc gtcggccgtc tttttcccac tctcgacgct gtcgaaccca
     3121 ctcaggacca cgcggtacca agttccctta ccgggcacct cgaccgcctc cacgcggggc
     3181 cggtatccca gggccgccac cttcttgttc aactgctccg ccttggcctt ctcctggtag
     3241 gagacgagct gcacaaggaa cttttccttt cccgcaaccg gcttttccgc aagccccgcc
     3301 ggtgccggtt tgcccgcacc gggaacggcc gccgcggccg gcggctgcgt ttggcccgcc
     3361 gtcgcggggg ccttggcggt gggagtcgcg ggcaatcggg ccgcgggaag ggaagccgac
     3421 ggcgccgacg gtgcgtcctt ggccgatggc ctcggggcga cgggacgctg gaggatgatg
     3481 tcgccctcct ttttcttgct cagggtatcg taaaacgtca gatcgaattc gtcctcttcc
     3541 gggggggcgg tctccttctt cgcctcgccg agaacgaccg gcgccgccgg cgccttccat
     3601 cccatccatt cccccaggac gccgggaaga tagccggcga tggccgcggg ataggtatcg
     3661 atgttcttcc cgaccgtcac cccgaagagg aaggttgcga atagcccggc cgccatgccg
     3721 aaaaccagga gcgagatccc cgtcttcccc agtttgactt caaattgctt ccggtttttc
     3781 gcgaccatgc gccaccgccg ttcggaaaca atcaggcacg gggccccgcc tttcatggaa
     3841 aacagggtgc aatccccgcc ctgctctaca tgcgctccgg cgccgatacc cccaggagcc
     3901 ccaggacgtt cgccaggacg gtttggaccg cccggacgag aaagaggcgc gccccagtga
     3961 ggtccccgtc ctcggagacc accttgttct tgttgtagta gctgtgaaac aggcccgcca
     4021 gctcgttgag atagaaggtc aggcggtggg gttccaggtt ccgcgccgcc ccctcgatca
     4081 cctccggcag gcggatcagg gtcttgatca gggccgcctc ctccgggagc gtcaggcgct
     4141 ccagggccgc ctgtccgtag gccggcacgg ccacgccttt ttcggcggcc gtcttcagga
     4201 tgctgcagat ccgcgcgtgg gcgtactgga cgtaatagac cgggttgtcg ttcgactggc
     4261 gtttcgccac ctcgaggtcg aaatccaggt ggctgtcgga gcgccgcatg aggaagttgt
     4321 agcgcgccgc gtcccgtccg acctcgtcga ccacctgacg cagggtgacg aactcgcccg
     4381 agcgcgtgga catggccacg ggcttgccgt ccctgaggag gttgacgagc tggacgagga
     4441 tgacctgcag ggcctccttc ggatacccca gggcctggac gcccgcgtgc atgcggggga
     4501 tgtagccgtg gtggtccgcg ccccagacgt cgatcaccgt gtcaaagccg cgctcgaact
     4561 tgtttttgtg gtaggcgatg tcggcggcga agtacgtggg ctccccgttc tggcggatga
     4621 cgacccggtc cttctcgtcg ccgaaggccg tcgtcctgaa ccacagggtg ccgtcctcgc
     4681 ggtagatgaa gccctgggcc tcgagcgccg ccagaagcct ggccacgccg tcgttctcgt
     4741 agagggtccg ctcgctgaag tagcggtcga agacgatccc gaaggccttc aggtcgtcct
     4801 tgatcccgtc gaggatgacc ccgccggcat ggtccgtcag gcgggggatg gcctcccctt
     4861 ccgcctcgtc gagacagcgc cggccctccc gcgcgaggac ctcccgcgcc agttcccgga
     4921 tgtactcgcc ctggtagccg ttctccggaa acggcgcgtc cccgccgtcc agctcccggc
     4981 agcgggcgag gaccgaccgg cccaggttgt gcatctggtt gcccgcgtcg ttgatgtagt
     5041 attcccgctc gaccgcatag ccgcaggcgg cgaggaccgc ggcgacggcg tcgcccacca
     5101 ccgccccccg ggcgtggccg atgtgcaggg ggcccgtggg gttggcgctg acgaactcca
     5161 cctggatgcg ctttccggcg ccgaagtcga gccggccgta gcccgtcccc tcgcgctcca
     5221 cggcctccag gagcgtcgcc cagaacccct cgcggatgaa gaaattgatg aagccggggc
     5281 cggcgatttc cgtccgggcg acaacacccc cgggatcggc cagctccgcg atcaaggcct
     5341 cggcgatccg ccgcgggttc atcttcaggc gcgaggccag catcatggcc gcgttcgtgg
     5401 cgtaatcgcc gtgcgcggcg tccttcgtga gttcgatctc caccgcgggg agttccgccg
     5461 ccgcgagcgt gcccttctcc atgcacgccg caagggcctc cccgaccatc cgttccaacc
     5521 gtttcttcat atccttaagc tcttaccggt ttgctccacg gtcccgtggt taccccgggt
     5581 ctgacgaagg gggtccgccg cgacccacat cgtccctttc gatcaaaaaa aagtgacaag
     5641 taaccctcca atcccggctg ttacttgtca cctcgctgtc ttacggtgct gagggcggac
     5701 gggctatccc tcacgttcct ttttcaccgc ctcttcgata tccttgtcct tgatggcaag
     5761 gtcccgggag taatcggcgc agtggacctg cccggtcgca tccgtggaca cgctgaattt
     5821 tttctgacag gtccctctcc aggcgcagat ggcgcagtac cttcgatcca tggccatgat
     5881 gcaatacccc cttcttgaag attcccccga cgggcggcgc aaccctaata cgaatgcccc
     5941 tttcaagtca aggatttttg cacgcagggg cgcttctccg gggcctctcg ccgcgtccgc
     6001 aaacgcgccg ataaaccgtt gaaaaacggt ttgacattcg gggagggttt tctttaagat
     6061 gccggtaaag tcctcattca aaaaggaagg cccgccatga gaaaatatct cctctggatg
     6121 tccgtcgtca tttcggtcct gttcctcccc gtcgtcaccg acgccgcctg cctgatccgt
     6181 ctcgagagcg gcggccggat ctggacgccc cactgctggg aggaaggtgg ggagctgaag
     6241 ttctgcgtgg gcgagggtgt tgcgggagtc gagaaaaaca tggtgcgtca catcgaaaaa
     6301 accgccatga cctcgggcgt cgtctatgaa gcgccgtcac cggcgcgggc ccccgccgat
     6361 accgcggaga aggccgaaaa aagcccctcg tctccggcca acgtcgagag cggcgcagcg
     6421 ctcccaaaac ctccggcaaa gcaagtcgat gtgaaggcct accaggaccg gatggccaag
     6481 ctcaagacgg acctgaaccg aacgctcgcg cggatgaaga aggccactgc aaataacgat
     6541 cctggcgaca aggaggaggc cacggcggaa aaccgccgga tctccgatga aatgtaccgt
     6601 ctgaccgatg aactgaagga aggcaacaac ggcgttctgc ccgccgactg gtgggaaggc
     6661 atcgggaagg aagagccggc ggaaccgtaa aactgggcag acgggccgta tccgcccctt
     6721 tagaagacat cccgccaggc cgtccgctgg agcgtggtaa tcgaattgat gttctgcagg
     6781 ttgctcaggg ccgaagaact gtaaaacagg ttgatgctgc cggtcaggtc gatcagcttg
     6841 ctctcgtggc cgatcgtgat catgctcccg aagatattgc cggtcccggc gccgaagacc
     6901 cggccggtcc ccctcaggat gaccagccct tcgaactcga aattgccgac gatgttgagc
     6961 tcacccccgt tgtcgacgat gatgatgccc gcaccgttcc cggtgccgtt taacgtggct
     7021 ccgttgggaa tgaccgtgat ggccggaatg tcccgcgtgc ccagcgtggt ctgatacagg
     7081 ttgttcgtca ggacattgtt gacgaaattg ttgtactccg tctcccggct gggccccagg
     7141 gtctgcgtgg ggctcccgcc caggtgggcg gaaacatttc ccgttacggt cggggtcatg
     7201 acggtgtaga ggcccggctt ggccggcagc gaactgtccg ccgtcgtgtc gcaggcgctg
     7261 ccgttgcaat tcggatcggc cggcaccggg taatcatggc cgtcgaccgc gtagcccccg
     7321 cccccgcctt tcttgaagtc gaccgccggc ccgccgccgt acatggcgac cgcgccgtcc
     7381 gcgctcgtgg tggacggggg aatcttccgg atgcgggcct cgatcgtctt cgaggtgttg
     7441 ttggtcccga tgccggtcat ctggaaccgg taccggtagg gcgccacata gtggagcgtc
     7501 accgtggggt tgaagctgaa gtcagccgga caggttacgg taaccgtcgt gggggacccg
     7561 gccgtgggat agacgaccgg attgttccgc aggtaataaa tgacctgctc cacccccgcc
     7621 tcggcctcgt aaaaggcctg ggtgccggcc ttgtagttcg cgctgatctt catgtccgtc
     7681 gtgctcatga tcacagccgt cgaaccgagc agcaccagga cagccgtcag gagcaggacg
     7741 accacgagga ccatgccccg ttcgtcggaa tgccgaactt ccttgcgggg ttcgatgagc
     7801 tgcgccatgc tgacacctcc cctgcctttc agatggcaag atttctggcc gtcacgacgg
     7861 acg
//
LOCUS       DAOOLJ010000077         7530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_11543_length_7530_cov_15.042140, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000077 DAOOLJ010000000
VERSION     DAOOLJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11543_length_7530_cov_15.042140"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            674..2407
                     /locus_tag="PLW16_09265"
     CDS             674..2407
                     /locus_tag="PLW16_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB71138.1"
                     /translation="MQDRRKTKAQLIDELNVLRDRDALCQRLLSSVGDAVLVLDGFRI
                     EDCNPRAQALFGRTREQLIGRTPLAVSPEKQPNGTPSARLGPELLAAVQPGEPLRCEW
                     QYLGPNGTPISTELVLHRLDGGSRVLAVSRDLTERKSVNDLYRALARNSPIGVFLAVG
                     GKFVYTNRKFCELTGYDRKELSGRPSGALVHPEDRDWARRNALLMLQGVVTEPFEFRA
                     VKKDGAISWTMETVSSITFRGRRAVLGNCMDVTRQKLTEAALRESEARYRTIIADIVD
                     GYYEVDLTGRFTFFNDRVWQMLGYPREETMGLSYLQYTPEAAARKVFEVFNRVYRTGE
                     PYHGLEWEIMRKDGTLGFGEASVSLIRDADGNPSGFRGIVRETTERRKMEEIIRNLAY
                     EDDLTGLPNRRAFLERFTREIARAGRHGRKFMIAMMDLDRFKEVNDTLGHPMGDRLLK
                     AVAQRIRGLLRKEDTVARLGGDEFALLIPDISDRTAGERVGDKLVAAFRSAFRVDPHV
                     LEITTSIGMAVYPDDGLDEEVLGRNADSALYRAKEKGRNGWAWREGHSADQFRQGDLP
                     FLNRSKGRESS"
     gene            2404..3408
                     /locus_tag="PLW16_09270"
     CDS             2404..3408
                     /locus_tag="PLW16_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPB71139.1"
                     /translation="MIRYRDLGLVNTRAMFAAAVHGGFAVPAYNFNNMEQLQAIVLAC
                     LETQSPVILQVSKGARDYADETLLRYLGQGAVAMMKRVAGEKGLGEIPITLHLDHGDS
                     YDLCVSCIETGFSSVMIDGSHLAYEDNCALTRRVVDYAHRFDVTVEGELGVLAGVEDD
                     VSAESHTYTKPEEVQDFLSRTGVDSLAISIGTSHGARKFKPGQDPEIRLDILREIEKR
                     IPGFPIVLHGSSSVPPEAVDTINRYGGRLQDSIGIPEAQLREAARSAVCKINIDSDGR
                     LVMTAAIREVFAVRPAEFDPRKYLGPAREALKAMYQRKNREVLGSAGKAAAILEAIGR
                     "
     gene            3436..4365
                     /locus_tag="PLW16_09275"
     CDS             3436..4365
                     /locus_tag="PLW16_09275"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB71140.1"
                     /translation="MRADHFAFKMADGVELFVYRWLPDDPAGATAKVQIAHGMAEHGG
                     RYERFAQALTAAGYAVYAPDHRGHGRTARSEDDLGYLADADGFNRVVEDHLALNGRLQ
                     KDLPGRPVYLFGHSMGATLAQVYISRYGEALRGAILSGTTARQGPLILFALGAARLEM
                     TFRGNRERSPLLTALSFGAFNRPFRPNRTEFDWLSRDTAEVDKYVADPLCGFTCTTSF
                     FRDLFTGVRVAHASATYAEVPAGLPILVFSGDQDPVGGFGKSVRQTVAAYREAGCRDV
                     TGKDYPGGRHEMLNETNRDAVTADILAWLAAHR"
     gene            complement(4432..5664)
                     /locus_tag="PLW16_09280"
     CDS             complement(4432..5664)
                     /locus_tag="PLW16_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPB71141.1"
                     /translation="MAKENQGVMPRWLPLVGGVLGSTTCGLLLYAWSVFIKPLNAEFG
                     WSRAEIALAFALCCLVFGLTTYPAGRLSDKFGPKVVVLTGGIILGIGFVLSGYIQAKW
                     QLYLTYGLVAGFGGGLVYLPPIATAPKWWPDRRALATGFAVVGLGLGSFIMAPFATYI
                     IDTIGWRYVFIYVGAAMGIMAVFAAFCLPVPPAGWKPAGWTPPAPAPGTAARKTRDYT
                     YQETFRTPQFWLLYVAYFCAAFAGLMVIGHIAGHGIDQGLSPMVAAGAASWLAIFNAV
                     SRILIGLVADKTGTRASFVGIFILQVVAMLLLYPSGSVYWALWIVAALIGWNYGSMFT
                     LFPATCLQYFGPTSQGTNYGLLFTAWGLAGFAGPFVGGWLKDMTGTYYIPFIVGAAVV
                     AISTFITALVKPPAKLGE"
     gene            complement(5834..>7530)
                     /locus_tag="PLW16_09285"
     CDS             complement(5834..>7530)
                     /locus_tag="PLW16_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB71142.1"
                     /translation="GGDFLMGSLFHTISREAAAELRLLKAMGYDAATFGNHEFDLKPA
                     GVAQMLSAASSTDGLPALVQANLRFSPNDPRDDALAERFAEGLVKPYIILVRDDRKIG
                     IFGLLGRGAAEMAPFAKPLVFADPIETARQMIDRLRNVEKVDWVVCLSHNGLGRMAGP
                     LNDVTLAREAPGIDVIVGGHSHTRLEAPLRVGDTVIVQAGANGRWVGVLDLERRDGRI
                     AVRAYRSVPIDARIPADPVIQRRIEAFAGIVDQNVLTAHDLSFRQIIAETAFDLTLKE
                     EESNLGNLMADALVWMANRHAFDPADTATRIVMAVESNGVIRDDLMKGRTGALAVTDV
                     FRAIPLGLGMDDTMGYPVIAVYLTGAEIKKALEVLTSIHPLKGDPYFLQVSGVRFTYN
                     PKRLIFDRVTDIRIGSAEEGWRPLDTTCRNKALYRVAANLYNATFLKLVGDFTWQILK
                     IVPKDRQGRPVENLADMRIDADPSRPGIQELKQWAGFMVYLRQFTDVDGDGLPDIPET
                     YRGKLGRIVPEPSLHPVDLLAHGSWLTWTAFGAGLLILTGLILGIRWLIRAARRRR"
BASE COUNT         1323 a         2442 c         2533 g         1232 t
ORIGIN      
        1 cgtccagatg gtccacctgc cagacgacgt tgtcgcccag ccgcagcccc tggaccaccg
       61 cgtccagatc ggacaggccg cttgtcaccg ttctgtcgtc catggtcctc cttccccgcc
      121 ttcggcgtcg ccttccggga tgggtccgcc cgcgacccgc cgtctccggt tgttttcgag
      181 gttctcccgg tccgcatcgc ggcgccgggg cgccgtgtcg aggggcgggg gccgacgcat
      241 ccccctgttg aaaagcaagc gcgatgccag gacggcctgg cggggcctcc atccggaagg
      301 ggcggccgtg gcttttcgac ctgccggatc agaggcgggg gcgcatcggt tcatcccttc
      361 ataaattcaa gcaattatgc attccaccgc aaggtcgtcc gaaggccggc gtgcgggtcg
      421 ccgacggagc gtgagggagt ccgacgcacc ccgggccgtc gcaaccgggc acaaacttgt
      481 ttcaattttg ccgccccgtc cggaatcggc gacagaaatg ttccaagaac ttttcgccgt
      541 cggaatattg acagtaaccc cctttgcggg tatgaaaagg catacgaggg gaacgattga
      601 tttatctccg cctgcttgtt gaatgattcc gtcatcggca cggaccgctc gcccggaacg
      661 gcacaccgca cccatgcagg atcgccggaa aacgaaagcg caactgatcg acgaattgaa
      721 cgtcctgcgg gacagggatg cgctgtgcca gcggctgctt tcctccgtcg gcgacgccgt
      781 gctcgtcctc gacgggttcc ggatcgagga ctgcaatccc cgggcgcagg ccctgttcgg
      841 acgcacgcgg gagcaactca tcggccggac gcccctggcc gtctccccgg aaaagcaacc
      901 gaacggcacc ccctccgccc ggctcggccc cgagctgctc gccgcggttc agccgggcga
      961 gccgctgcgg tgcgaatggc aatatctcgg cccgaacgga accccgatca gcacggaact
     1021 ggttctccac cggctcgacg gcggctcccg ggtgctggcc gtttcccgcg acctgacgga
     1081 gcgcaagagc gtgaacgatc tctaccgcgc cctggcccgc aattccccca tcggggtgtt
     1141 cctggcggtc ggcgggaagt tcgtctacac caaccgaaaa ttttgcgaac tgaccggcta
     1201 cgaccggaaa gagctgtcgg gccgccccag cggggcgctc gtccatcccg aggaccggga
     1261 ctgggcgagg cggaatgccc tgctgatgct tcagggcgtc gtgaccgaac ccttcgaatt
     1321 ccgggcggtc aaaaaggacg gcgcaatctc ctggaccatg gaaacggtga gctccatcac
     1381 cttccgaggg cggcgggccg tcctcggcaa ctgcatggac gtgacccggc agaaactcac
     1441 cgaggccgcc ctgcgggaga gcgaggcgcg ctaccgaacg atcatcgccg atatcgtcga
     1501 cgggtattac gaggtcgacc tgaccggccg cttcacgttc ttcaacgacc gggtctggca
     1561 gatgctgggc tatccccgcg aagagacgat gggcctgagt tacctccagt acacccccga
     1621 agcggccgcc aggaaggtct tcgaggtgtt caaccgggtc taccgaacgg gcgaacccta
     1681 tcacggcctg gagtgggaga tcatgcgcaa ggacggcacc ctgggattcg gcgaggcctc
     1741 ggtttcactg atccgggacg ccgacggaaa cccgtcgggc ttccgcggga tcgtccggga
     1801 gacgacggag cgccggaaga tggaggagat catccgcaac ctggcctacg aggatgatct
     1861 gacgggattg cccaaccggc gggcgttcct ggagcgcttt acccgggaga tcgcccgggc
     1921 cggccgtcac gggcgcaaat tcatgatcgc catgatggac ctcgaccggt tcaaggaggt
     1981 caacgacacc ctgggccacc cgatggggga ccgcctgctc aaggccgtcg cccaacggat
     2041 tcggggcctg cttcgcaagg aggacacggt ggcgcgcctg gggggcgacg agttcgcgct
     2101 tctgattccg gacatctccg acaggaccgc cggagagcgg gtcggcgaca agctggtcgc
     2161 ggcctttcgg tccgccttca gggtcgatcc gcatgtcctg gagatcacca cgagcattgg
     2221 catggccgtc tatcccgacg acggccttga cgaggaggtg ttgggccgga atgccgacag
     2281 cgccctctac cgggccaagg agaaaggccg caacggctgg gcgtggcgcg agggccattc
     2341 cgccgatcag ttccgccagg gggatttacc cttcttaaac cgttcaaaag ggagggaatc
     2401 gtcatgatcc gttatcgaga ccttggcctc gtcaacacgc gggccatgtt cgccgccgcc
     2461 gtccacgggg gcttcgcggt tccggcctac aacttcaaca acatggagca gctccaggcg
     2521 atcgtcctgg cctgtctcga aacccagtcg cccgtcatcc tccaggtctc caagggggcc
     2581 cgggactacg ccgacgagac cctgctgcgc tatctgggcc agggagccgt ggcgatgatg
     2641 aagcgggttg cgggggaaaa gggcctgggc gagatcccca tcaccctcca tctggaccac
     2701 ggggattcct acgacctgtg cgtttcctgc atcgagacgg gcttctcctc cgtcatgatc
     2761 gacggctccc acctcgccta cgaggacaac tgcgcgctca cgcgccgcgt cgtggactac
     2821 gcccaccggt tcgacgtgac ggtcgagggg gaactggggg tgctcgccgg ggtggaggac
     2881 gacgtctcgg cggaaagcca cacctatacg aagccggagg aggtccagga ttttctgtcc
     2941 cgcacgggcg tggattccct ggccatctcc atcggcacct cccacggcgc ccgcaagttc
     3001 aagcccggac aggatccgga gattcgcctc gacatcctgc gggagatcga gaagcgcatc
     3061 cccggctttc ccatcgtcct gcacggatcc tcctccgttc cgccggaggc ggtggacacg
     3121 atcaaccggt acggtggccg cttgcaggac tccatcggga ttcccgaggc gcagcttcgg
     3181 gaggcggccc ggtcggccgt ctgcaagatc aacatcgact ccgacggccg cctcgtcatg
     3241 acggcggcga tccgggaggt ctttgccgtc cggcccgcgg agttcgatcc ccgcaaatat
     3301 ctgggtcccg cgcgggaggc cctgaaggcg atgtatcagc gcaagaacag ggaggtgctg
     3361 gggagcgccg gcaaggccgc ggcgatcctc gaagcgatcg ggagatgagg accagacctt
     3421 aaaggagggg acgggatgcg cgcggatcat tttgcattca aaatggcgga cggggtggag
     3481 ctcttcgtgt accggtggct tcccgacgat cccgccgggg cgacggccaa ggttcagatc
     3541 gcccacggca tggcggagca cggcggtcgg tacgaacgct tcgcccaggc cctgaccgcg
     3601 gccggctacg ccgtctatgc gcccgaccat cgggggcatg gacggacggc ccgatccgag
     3661 gacgacctgg gctatctggc cgatgcggac ggcttcaacc gggtggtgga agaccacctg
     3721 gcgttgaacg gccgcctgca gaaggatctc ccggggcggc cggtctatct tttcggccac
     3781 agcatgggcg ccacgctggc ccaggtttac atcagccggt acggcgaggc tctgcggggg
     3841 gcgatcctgt ccggaacgac ggcccgccag gggccgctca tccttttcgc cctcggcgcg
     3901 gcgcgtctgg agatgacatt ccgggggaac cgggaacgca gcccgctttt gacggccctg
     3961 tccttcgggg ccttcaaccg gccgttccgg ccgaaccgga cggagttcga ctggttgagc
     4021 cgggacacgg cggaggtgga caagtacgtt gccgaccccc tctgcggctt cacctgcacg
     4081 acctcgtttt tccgcgacct tttcaccggc gtccgggtcg cgcacgcctc cgccacctat
     4141 gccgaggttc ccgccgggct gccgatcctc gttttctccg gcgatcagga tcccgtgggc
     4201 gggttcggaa agagcgtccg gcagacggtg gcggcctatc gcgaggcggg ctgccgggac
     4261 gtgacgggga aggattatcc cggcgggcgt catgagatgc tcaacgagac caatcgggac
     4321 gcggtcacgg cggacatcct ggcctggctt gccgcccacc gctgatccgc ccggtcggac
     4381 cgtccgcgga cggaatgacg gcccgtgggt tgccacgggc cgtccgcgtt tctattcgcc
     4441 gagcttcgcc ggcggcttca ccagcgccgt gatgaaggtg gagatcgcca cgacggcggc
     4501 cccgacgatg aacgggatgt agtaggtccc cgtcatgtcc ttcagccagc ccccgacaaa
     4561 cggaccggca aatccggcga gtccccaggc ggtgaagagc agcccgtaat tcgttccctg
     4621 ggaggtgggg ccgaagtact gcaggcacgt tgccgggaac agggtgaaca tcgaaccgta
     4681 gttccagccg atcagggccg ccacgatcca cagcgcccag tagaccgatc ccgacggata
     4741 caggagcagc atggccacga cctggaggat gaagatcccc acgaaggagg cccgggttcc
     4801 cgtcttgtcg gcgacgaggc cgatcaggat ccgtgagacg gcgttgaaga tggcgagcca
     4861 gctcgcggcg ccggcggcga ccatcgggga gagcccctgg tcgatgccgt gcccggcgat
     4921 gtggccgatg accatcagtc ccgcgaacgc cgcgcagaag taggcgacgt agaggagcca
     4981 gaactggggg gtgcggaagg tttcctggta ggtgtagtcc cgcgtctttc gggcggcggt
     5041 tcccggcgcc ggcgccgggg gcgtccagcc ggcgggtttc cagcccgcgg gcggcacggg
     5101 caggcagaag gcggcgaaga ccgccatgat ccccatggcc gccccgacgt agatgaagac
     5161 gtagcgccag ccgatcgtat cgatgatgta cgtcgcgaag ggcgccatga tgaaggagcc
     5221 cagccccagg cccacgacgg caaaccccgt ggcgagcgcg cgccggtccg gccaccactt
     5281 cggggccgtg gcgatggggg ggagatagac caggccgccg ccgaaaccgg cgacgaggcc
     5341 gtaggtcagg tagagctgcc attttgcctg gatgtagccg gacaggacga acccgatccc
     5401 cagaatgatc ccgcccgtca agacgacgac cttcggcccg aatttgtcgc tcaggcgccc
     5461 ggccgggtag gtcgtcaggc cgaagacaag gcagcacagg gcgaaagcca gggcaatctc
     5521 cgcccggctc cagccgaact ccgcattcag cggtttgatg aaaacgctcc acgcatacaa
     5581 gaggagaccg cacgtcgtgc tccccaaaac gcctcccacg aggggaagcc atctcggcat
     5641 gaccccctga ttctccttcg ccatcgctgt tgtccctcct tcgtgatgaa tggtgtgaat
     5701 gcaacatgcg gaaaatccga cgtccgcggc cacgaccatc gggagattcc acagtgcggt
     5761 tcaatcaact gatggaataa agaatggtct tttaaaagtc aagcaaaaac aaattgctga
     5821 gaggaaggat tgactatcgc cgccgccgcg ccgcgcgaat cagccagcgg atcccgagga
     5881 tcaggccggt caggatgaga agaccggcgc cgaaggccgt ccaggtcagc caggatccgt
     5941 gcgccaggag gtcgacggga tggaggctgg gctccgggac gatccggccg agttttcccc
     6001 ggtaggtctc cgggatgtcg ggcaggccgt cgccgtcgac atcggtaaac tgcctcaggt
     6061 agaccatgaa cccggcccac tgcttaagtt cctggatccc cggacgcgac gggtccgcat
     6121 cgatccgcat gtcggccaga ttctcgacgg ggcggccctg gcggtccttg ggaacgatct
     6181 tcaggatctg ccaggtgaag tcgccgacca gcttgagaaa ggtggcgttg tagagattgg
     6241 cggctacgcg gtacagcgcc ttgttccggc aggtggtgtc gagcggccgc cagccttcct
     6301 ccgccgagcc gatccggatg tccgtcacgc ggtcgaagat caggcgtttc ggattgtagg
     6361 tgaaccggac gccggagacc tgaaggaagt agggatcgcc cttgagtggg tggatgctcg
     6421 tcagcacctc gagggccttc ttaatttccg cgccggtgag atagaccgcg atgaccggat
     6481 agcccatcgt gtcgtccatc ccgaggccca gcgggatcgc ccggaagacg tccgtcacgg
     6541 cgagcgcgcc ggtccggcct ttcatcaggt cgtcccggat gacgccgttc gattcgacgg
     6601 ccatgacgat tctcgtcgcg gtgtccgccg gatcgaaggc gtggcggttc gccatccaga
     6661 cgagggcgtc tgccatgagg ttgcccaagt tggattcctc ttcctttagg gtcaggtcga
     6721 aggccgtctc ggcgatgatc tggcggaagg acaggtcgtg cgccgtcagg acgttttggt
     6781 cgacgatacc ggcgaaggcc tcgatccgcc gctggatcac cggatcggcg gggatgcgcg
     6841 cgtcgatcgg gaccgaccgg tacgcccgga ccgcgatccg tccgtcccgt cgctccaggt
     6901 cgagcacgcc gacccagcgg ccgttggcgc cggcctgaac gatcaccgtg tcacccacgc
     6961 ggagcggcgc ctccaagcgg gtgtggctgt ggcctccgac gatgacgtcg atccccggcg
     7021 cctcccgggc cagggtgacg tcgttcagcg gtccggccat gcggcctagg ccgttgtggg
     7081 acaggcagac gacccagtcc accttctcga cgttccggag gcggtcgatc atttggcggg
     7141 cggtctcgat ggggtccgcg aagacgagcg gtttggcgaa aggcgccatc tcggccgccc
     7201 cccgcccgag gaggccgaag atcccgatct tccggtcgtc cctgacgagg atgatgtagg
     7261 gcttcacgag gccctcggca aaccgctcgg ccagggcgtc gtcccgggga tcgttcgggc
     7321 tgaagcggag gttcgcctgg acgagcgccg gcaggccgtc cgtggagctt gccgcggaga
     7381 gcatctgcgc cacgcccgcg ggcttcagat cgaactcgtg gttgccgaag gtggccgcgt
     7441 cgtagcccat cgccttgagc agccggagtt ccgcagccgc ctcgcggctg atcgtgtgga
     7501 agagggaccc catgagaaag tcgccgccgt
//
LOCUS       DAOOLJ010000078         7321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_12032_length_7321_cov_18.366639, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000078 DAOOLJ010000000
VERSION     DAOOLJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12032_length_7321_cov_18.366639"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLW16_09290"
     CDS             <1..462
                     /locus_tag="PLW16_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71143.1"
                     /translation="IVKGPQIMLGYKGKPEETANTLKDGWIYTGDIATMDEDGYFYIV
                     DRKKDMIISGGYNVYPRDIDEVYYENPKVQEACSIGIPDPKRGENVKLFIVLKEGQTA
                     TQEEMIEYGKTKLAAYKLPSEVEFRKELPKTNVGKVLRKELRAEELKKRGK"
     gene            complement(617..1765)
                     /locus_tag="PLW16_09295"
     CDS             complement(617..1765)
                     /locus_tag="PLW16_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB71144.1"
                     /translation="MNFAPTEEQAMVRDMVRKFAEAEIKPIAEELDHTHRHPEEICRK
                     LGEMGIMGVAVPTEYGGAGMDNVTYVMAMIEISKACASCGVIVSVNNTLYGHPVKTFG
                     THEQKLEYLTPVAGGQVEGCYGLTESGAGSDAASLKCRAELKGDKYIVNGAKTFITNG
                     PVARYCVLAATTDPSIGYKGVINLIVDMKNTPGFKVGKIEEKMGILASGTSELIFEDA
                     EVPAANLLGKTGQGFQQMMAGLNAGRIGIGAQACGIGRAALEDALAYAKERVQFGKPI
                     STFQAIQFKLADMATELDAAELLLLRAAWMEDNHMDFEMQSAMSKYYSSDVAMKAAIE
                     GVQIFGGYGYIKEYPAERHMRDAKICQIYEGTNEIQRVVVARKLLGQR"
     gene            complement(1827..2609)
                     /locus_tag="PLW16_09300"
     CDS             complement(1827..2609)
                     /locus_tag="PLW16_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPB71145.1"
                     /translation="MDYKNILFDVAEGVATITFNRPKALNAMNAETMTELRDAATVCK
                     NDDAVKALILTGSGEKAFVAGADISQMQSLNAPQALAFMELGHETLRLIETMPKPSIA
                     AVNGFALGGGTEISLACDMRFAAETAVFGQPEILIGLIPGWGGTQRLPRIVGMGRAKE
                     LVMGGAQIKAQRAYEIGLVNRLYPLETLMDETRKFALKLTQLPGFALKMAKHSINFGY
                     DLALDNANRLEVECCAQCFSTNDQKEGMQAFLEKRKPVFTGK"
     gene            complement(2738..>4000)
                     /locus_tag="PLW16_09305"
     CDS             complement(2738..>4000)
                     /locus_tag="PLW16_09305"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPB71146.1"
                     /translation="ARTAVGSFGGSLKGVRTVDLGALVIKEAIKRAGLRPVVSDFVNG
                     CRPDVFKDVTMTEIQKKYYDYADTLAPAYFDECIMGNVLQAGLGQNPGRQASIYAGLP
                     EETNTITVNKVCASGMKAITLAAQIIKAGDAEILVAGGMENMSNAPFALPDARWGYRM
                     SMPFGQITDIVVHDGLYEIFNKYHMGFTAENIAAKYGITRQEQDELALMSHQRARAAI
                     ASGAVDDEIVPVVIPSKKGDTVFKVDERPMDTTLEKMAKLPPAFKRDGGTVTAGNASG
                     INDGAAAVVVMSAEKAKELGLTPLAQIKGYASGGVDPAYMGLGPIPATRKVLRKLGLT
                     MKDIDLVELNEAFACQAIACIRELGVDLDKANLNGSGISIGHPVGCTGARITYSLAMQ
                     MKKKDLNLGLATLCIGGGQGMAIVLERV"
     assembly_gap    4001..4100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4101..4774)
                     /locus_tag="PLW16_09310"
     CDS             complement(<4101..4774)
                     /locus_tag="PLW16_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPB71147.1"
                     /translation="MTKTPQKLNHKMAELYRGKPVAAYGERRTDFSGSGRKIENAFKA
                     LREVVLVEACRTPYGRAGGALKGFTAPELGALAIKEVLERTGGKVKPEDVDYVFMGQV
                     VPAGAGQVPSRQATILAGLPESVPSITVNKVCSSGIKTVDLACQMIQLGRAEICIAGG
                     QESMSNCPFSLPDMRWGARMGLPSKPAVDLMVNDGLWCAFYNRHMALHGSEVADEFGF
                     SRADQD"
     gene            complement(4767..6227)
                     /locus_tag="PLW16_09315"
     CDS             complement(4767..6227)
                     /locus_tag="PLW16_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71148.1"
                     /translation="MTLGDLLRDAVERYPAHTALIHESRTWTYGEFEGAVRALGHHLS
                     GLGIGKGDHVALMLPNCPEFVIAYFAIVNLGAVAVTLNAQSTAHELKHLLGDSDALAF
                     ITTAPAARRYEEIRDVVPACRHLLVANGPDSPFGKILAGAVPALDPPEIAPDDPAVMI
                     YTSGLTGKPLGAMLTHRNLYTQSALLRDIYGGTENSRSLAIIPLFHSFGAVANMLAAM
                     RTGAGIVLMERFTLDGIFQTVARERITYLAAVPRLFLGMLFHEGAEKYDVGSLEFCIT
                     GGSAMPPEFIPLFEDKFKVILREGYGLTEASPVCSVVRREKMQKVGSIGTVIPGAEAR
                     IFDEDDREVPRGVEGELVIRGENVMKGYYKAPEATAQVLRNGWLHTSDLAKMDEDDYI
                     YITGRKKRMIITSGFNVYPREVEIAIELHPAVAKARIESKPDLIRGEIVKAFIQRRPD
                     AALDDKEIMKHCRTYLSSYKVPREVEFVNAIEEKDD"
     gene            6429..6959
                     /locus_tag="PLW16_09320"
     CDS             6429..6959
                     /locus_tag="PLW16_09320"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HPB71149.1"
                     /translation="MKKAILAVLLVLLWTASAAMAAELKVGDKAADFSLKDSEGKTFS
                     LQSPEFKGKVISVFYAGVSEGDDNNAAQDALHNDKGLDRKTGYAGFGIANLKSSIVPN
                     FVIKSAIKKKKDKTGAVILLDPDYTMINLWGLKNKASNVVILDKNRICRYIYKGKMPQ
                     AEVAKMLSVIKEYQVK"
     gene            7068..>7321
                     /locus_tag="PLW16_09325"
     CDS             7068..>7321
                     /locus_tag="PLW16_09325"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPB71150.1"
                     /translation="MKKIAFVSVVLGALLFATAHVWAASPVGKWKTIDDETGKAKSII
                     EVYEQNGKIYGKILESLKPGGGKGEVCDKCEGADKGKPIIG"
BASE COUNT         1218 a         2214 c         2396 g         1393 t
ORIGIN      
        1 atcgtcaagg ggccgcagat catgctgggg tacaagggca agccggaaga gacggccaac
       61 accctgaagg acggctggat ctacacgggc gacatcgcga cgatggacga ggacggctat
      121 ttctacatcg tggaccggaa gaaggacatg atcatctccg gcggctacaa cgtctacccg
      181 cgcgacatcg acgaggtcta ctacgagaac ccgaaggtcc aggaggcctg ctccatcggg
      241 atccccgatc ccaagcgggg cgagaacgtg aagctcttca tcgtcctgaa ggagggccag
      301 acggcgaccc aggaggagat gatcgagtac ggcaagacga aactggccgc ctacaagctg
      361 ccgtcggagg tcgagttccg caaggaactt cccaagacca acgtgggcaa ggtcctgcgc
      421 aaggagctgc gcgcggaaga gctcaagaag cgcgggaagt aagggatttc gtgaagacaa
      481 aaaggcgttg ggacgggaag gagtttcctg gtcccaacgc ctttgtttta tcgcccggca
      541 tcgcgttgcc gcccgggccc gacacggggt ctattgcctg aagcccgccg ccgtggatgc
      601 ggcgcgcggg gtttttttat ctctgtccca agagcttgcg cgccacgacg acgcgctgga
      661 tctcgttggt gccctcgtag atctggcaga tcttggcgtc gcgcatgtgc cgctccgccg
      721 ggtattcctt gatgtacccg tacccgccga agatctgcac cccctcgatc gccgccttca
      781 tggcgacgtc ggaggaatag tacttcgaca tcgccgactg catctcgaag tccatgtggt
      841 tgtcctccat ccaggcggcg cgcaggagca ggagctccgc cgcgtcgagc tcggtggcca
      901 tgtcggccag cttgaactgg atggcctgga aggtgctgat gggcttgccg aactggaccc
      961 gctccttggc gtaggccagc gcgtcctcga gggcggcccg gccgatcccg caggcctggg
     1021 cgccgatccc gatgcggccc gcgttcaggc ccgccatcat ctgctggaag ccctggccgg
     1081 tcttgcccag gaggttggcc gcggggacct cggcgtcctc gaagatcagc tcggaggtgc
     1141 cggaggccag gatgcccatc ttctcctcga tcttgccgac cttgaagccc ggggtgttct
     1201 tcatgtccac gatcaggttg atgaccccct tgtagccgat ggaggggtcg gtcgtggcgg
     1261 cgagcacgca atagcgggcg acggggccgt tcgtgatgaa ggtcttggcg ccgttgacga
     1321 tgtatttgtc gcccttgagt tccgcccggc acttgagcga ggcggcgtcg gagcccgcgc
     1381 cggactccgt gagcccgtag cagccctcga cctggccgcc ggcgacgggg gtcaggtact
     1441 ccagcttctg ttcgtgcgtc ccgaaggtct tgacggggtg gccgtacagg gtgttgttga
     1501 ccgagacgat gacgccgcag ctcgcgcagg ccttggagat ttcgatcatg gccatgacgt
     1561 aggtcacgtt gtccatcccg gccccgccgt actccgtggg gacggcgacc cccatgatgc
     1621 ccatttcgcc cagcttccgg cagatctcct ccgggtgacg atgggtgtgg tccagttcct
     1681 ccgcgatggg cttgatctcc gcctcggcga atttccggac catgtcgcgt accattgcct
     1741 gttcttccgt gggtgcaaaa ttcatgtctc atccctcccg aatcgatggg tgaagcggtg
     1801 aggcagcccc gcttcgcccg gaaatattac ttgccggtaa aaaccggctt gcgtttttcg
     1861 aggaaggcct gcatgccctc cttctggtcg ttcgtgctga agcactgggc gcagcactcc
     1921 acctcgaggc ggttcgcgtt gtccagcgcc agatcgtagc cgaagttgat gctgtgcttg
     1981 gccatcttga gggcgaagcc cgggagctgc gtcagcttga gggcgaactt gcgcgtctcg
     2041 tccatgagcg tctccagcgg atagagccgg ttgacgaggc cgatctcgta ggcccgctgg
     2101 gccttgatct gcgcgccgcc catgacgagc tccttggccc ggcccatccc gacgatccgg
     2161 gggagccgct gggtgccgcc ccagccgggg atcaatccga tgaggatctc cggctgaccg
     2221 aagacggcgg tctccgcggc aaagcgcatg tcgcaggcga gcgagatctc cgttccgccg
     2281 cccagggcga agccgttgac cgcggcgatc gagggcttcg gcatcgtctc gatgagccgc
     2341 agcgtctcgt gcccgagctc catgaaggcc agggcctggg gggcgttgag cgactgcatc
     2401 tgggagatgt ccgccccggc gacgaaggcc ttctcgccgc tgcccgtcag gatcagcgcc
     2461 ttgaccgcgt cgtcgttctt gcagacggtg gccgcgtcgc gcagctccgt catcgtttcc
     2521 gcgttcatcg cgttcaaggc cttgggccgg ttgaacgtga tcgtcgcgac gccctccgcg
     2581 acgtcaaaca ggatgttctt gtagtccatc gatcactcct tcaaatcttt gaaaatcgcg
     2641 atccatccac gcgccggaga tgtttcgatt ccgaaatatc tccgaggcgc gcttccggga
     2701 tccgatgtcc gggttgctca aaggcttcgc ccggtgtcta gacgcgttcc aggacgatgg
     2761 ccatcccctg gccgccgccg atgcacaggg tagcgagccc gaggttcagg tccttctttt
     2821 tcatctgcat ggcgaggctg taggtgatcc gggcgcccgt gcagccgacc gggtggccga
     2881 tggagatccc gctgccgttc aggttggcct tgtccagatc gacgccgagc tcgcggatgc
     2941 aggcgatcgc ctggcaggca aaggcctcgt tcagttcgac gagatcgatg tccttcatcg
     3001 tgaggcccag cttgcgcagg accttgcggg tggccgggat ggggccgagg cccatgtacg
     3061 ccggatcgac gccgccggag gcgtaaccct tgatctgggc caggggggtg aggcccagtt
     3121 ccttcgcctt ctcggcgctc atcacgacga ccgccgccgc cccgtcgttg atcccggagg
     3181 cgttcccggc cgtgaccgtg ccgccgtcgc gcttgaaggc ggggggcagc ttcgccatct
     3241 tctcgagggt cgtgtccatc ggccgctcgt ccaccttgaa gacggtgtcg cccttcttcg
     3301 aggggatgac gacggggacg atctcgtcgt ccacggcgcc ggaggcgatg gcggcccggg
     3361 cgcgctggtg gctcatcagc gcgagctcat cctgctcctg gcgggtgatg ccgtatttgg
     3421 cggcgatgtt ctcggcggtg aagcccatgt ggtacttgtt gaagatctcg tacaggccgt
     3481 catggacgac gatatccgtg atctggccga agggcatgct catccggtag ccccagcggg
     3541 cgtcgggcag ggcgaagggg gcgttcgaca tgttctccat gccgcccgcg accaggatct
     3601 ccgcgtcgcc ggccttgatg atctgggcgg cgagcgtgat cgccttcatg ccggaggcgc
     3661 agaccttgtt gaccgtgatc gtgttcgtct cctcggggag ccccgcgtag atgctcgcct
     3721 ggcggcccgg attctggccg aggccggcct ggaggacgtt ccccatgatg cattcgtcga
     3781 agtacgcggg cgcaagcgtg tcggcgtagt cgtagtactt cttctggatc tccgtcatgg
     3841 tcacgtcctt gaagacgtcc ggacggcagc cgttcacgaa gtcgctcacg acgggacgca
     3901 ggcccgcgcg cttgatggcc tccttgatca cgagggcgcc cagatccacg gtgcgcaccc
     3961 ctttgaggga ccctccgaaa ctgccgacgg cggtcctcgc nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn tcgtcctggt cggcccggct gaagccgaat tcgtcggcga
     4141 cctcggagcc gtggagggcc atgtggcggt tgtagaaggc gcaccacagg ccgtcgttga
     4201 ccatcaggtc cacggccggc ttggagggca ggcccatccg ggccccccag cgcatgtcgg
     4261 ggaggctgaa ggggcagttg ctcatgctct cctgcccgcc ggcgatgcag atctcggcgc
     4321 ggccgagctg gatcatctgg caggcgagat cgacggtctt gatccccgag gagcagacct
     4381 tgttcacggt gatgctgggc acggactccg gaagcccggc gaggatcgtg gcctggcggc
     4441 tcggaacctg gccggcgccg gcggggacga cctgccccat gaagacgtag tccacgtcct
     4501 ccggcttcac ctttccgccg gtgcgctcca ggacctcctt gatcgcgagc gcgcccaatt
     4561 ccggggccgt gaagcccttc agcgccccgc cggcgcggcc gtagggcgtc cggcaggcct
     4621 cgacgaggac gacctcgcgc agggctttga atgcattttc gatcttgcgg ccggacccgg
     4681 aaaagtccgt ccgccgttcg ccgtaggccg ccaccggctt gccgcggtag agctcggcca
     4741 tcttgtggtt gagtttctgt ggggttttag tcatctttct cctctatcgc atttacaaat
     4801 tccacttccc gggggacctt gtagctcgac aggtacgtcc ggcagtgttt catgatctcc
     4861 ttgtcgtcca gggcggcgtc cggtctgcgt tggatgaagg ccttgacgat ttcgccccgg
     4921 atcaggtcgg gcttgctctc gatccgggcc tttgccacgg ccgggtgcag ctcgatggcg
     4981 atttccacct cgcgggggta gacgttgaag ccgctcgtga tgatcatccg cttcttgcgc
     5041 cccgtgatgt agatatagtc gtcctcgtcc atcttcgcca ggtcgctcgt gtggagccag
     5101 ccgttccgga ggacctgggc cgtcgcctcg ggggccttgt agtacccctt catgacgttc
     5161 tcgccccgga tcacgagttc gccctcgacg ccccggggaa cctcccggtc gtcttcgtcg
     5221 aagatcctgg cctccgcgcc cggaatgacc gtgccgatgg agccgacctt ctgcatcttc
     5281 tcccggcgga cgacggagca gaccggggag gcctccgtaa gcccgtagcc ctcccggagg
     5341 atgaccttga acttgtcctc gaagagcgga atgaactcgg gcggcatcgc ggaaccgccg
     5401 gtgatgcaga attccaggga gccgacgtcg tatttctccg ccccctcgtg gaacagcatg
     5461 cccaggaaca ggcgcggaac ggcggccagg taggtgatcc gctcccgggc gacggtctgg
     5521 aagatgccgt ccagggtgaa gcgctccatg agcacgatcc ccgccccggt gcgcattgcc
     5581 gccagcatgt tggccaccgc gccgaaggaa tgaaacaggg ggatgatggc gagcgaccgg
     5641 ctgttttccg ttccgccgta gatgtccctg agcagggcgg actgggtgta gaggttgcgg
     5701 tgcgtgagca tggcgcccag gggcttgccc gtgagcccgg aggtgtagat catgacggcc
     5761 ggatcgtccg gcgcgatctc tggggggtcg agggcgggca ccgcccccgc gaggattttc
     5821 ccgaaggggg aatccggtcc gttggcgacg agcaggtggc ggcaggccgg gacgacgtcg
     5881 cggatctctt catagcgccg cgccgcgggc gcggtggtga tgaacgcgag ggcgtcgctg
     5941 tcgccgagca ggtgcttgag ttcatgcgcc gtggactggg cgttcagggt cacggcgacg
     6001 gcccccaggt tcacgatcgc gaagtaggca atgacgaatt cggggcagtt ggggagcatc
     6061 agggcgacgt gatcgccctt cccgatgccg agaccgctca ggtgatggcc cagggcgcga
     6121 acggcccctt cgaattcccc gtaggtccag gtccggctct cgtgtatcag ggcggtatgg
     6181 gcgggatagc gttcaaccgc gtcccgcaat aggtctccca aggtcatgat cttccgtaac
     6241 ccccgcaatt tttggccttt cccttagcat aggggcggtg tttttgccaa caaaagatgg
     6301 gggctgattt tctcttgatt ttttccttgc cccgcctgtc cgatttccct ataatgcgcc
     6361 cgtcctcccg gatggcgggg acgaatcaag gtccgaatca tccgaaaaaa caaaaaaagg
     6421 agaagggcat gaagaaagcg attctggcgg tgctgttggt tctgttgtgg acggcttcgg
     6481 cggcgatggc ggccgaactc aaggtgggcg acaaggcggc ggatttttct cttaaagatt
     6541 ccgagggaaa aacgtttagc ctccaatccc ccgaattcaa gggcaaggtc atctctgtct
     6601 tctatgcggg cgtcagcgaa ggggacgaca acaacgccgc ccaggacgcc cttcacaacg
     6661 acaaggggct ggaccgcaag accgggtatg ccggtttcgg tatcgcgaac ctgaaatcgt
     6721 ccatcgtccc caatttcgtc atcaagtccg cgatcaagaa gaagaaggac aagaccgggg
     6781 ccgtcatcct gctcgacccg gattacacca tgatcaacct ctgggggctg aagaacaagg
     6841 cctcgaacgt ggtcattctc gacaagaacc gtatctgccg ctacatctac aaggggaaga
     6901 tgccgcaggc ggaggtcgcc aagatgctgt ccgtgatcaa ggagtatcag gtaaagtagc
     6961 gcttcgtctg atccgacttc aatgcctgga cggtgtgcga cgggggcggt ccccgtcgcg
     7021 tacgcggggc cgaaagtccg acttttgaga aaagggaggg agtgccgatg aagaagattg
     7081 catttgtgtc ggtggtgttg ggggcgctgc tgtttgcgac ggcgcacgtc tgggcggctt
     7141 cgccggtcgg aaaatggaag acgatcgacg atgaaaccgg gaaggccaaa tccatcatcg
     7201 aggtttacga acagaacggc aagatctacg gcaagatcct cgaatccctg aagccgggcg
     7261 gcggcaaggg cgaggtgtgc gacaagtgcg aaggcgccga caagggcaag ccgatcatcg
     7321 g
//
LOCUS       DAOOLJ010000079         7224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_12238_length_7224_cov_17.851444, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000079 DAOOLJ010000000
VERSION     DAOOLJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12238_length_7224_cov_17.851444"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLW16_09330"
     CDS             <1..371
                     /locus_tag="PLW16_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPB71151.1"
                     /translation="LARRLLGPRAIIGVSVETWEDVEQAQALDCDYLGVSPVFATPTK
                     TDTRGAWGLEGLAKIRAFSRHPLVAIGGINAANAEAVCRAGADSLAVVSAICAAADPF
                     QASQGLAAVIHRVREERGVR"
     gene            368..1528
                     /locus_tag="PLW16_09335"
     CDS             368..1528
                     /locus_tag="PLW16_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HPB71152.1"
                     /translation="MKTLVVFDYSGTLSLAAVAFGRPDRLREQLDASGLAALGIASPG
                     VFWDEVVGPTWTEGSTTPVGYRGVMARRIRERCGVRPGDPADDRIRDAVSRFVGAYLG
                     QSRIDLRWQPLLNALTSNPDVQLLVATDHYAEATDYIVGYLRDGGLTARAIRDEPEAP
                     AAPARILVANSADLGAPKAARAFWEQSRRLLPARMDRVILIDDFGFNEQAGDSYGARQ
                     KVAERMAKTAFLLEEVFDGAVEVVPFMIEPGLPAGAAGAVIRETDGQVRALLSGTAGG
                     SPSPSARQTENPAPGGSGLQPPREPPACKRKPDLTYPCPWVYKVVGADPDALRQAIAA
                     VVGPRRHTVTPSRTSANGKYHSLNLALEVRDEAERLTLYDGLRRQPAVRIVL"
     gene            1779..2327
                     /locus_tag="PLW16_09340"
     CDS             1779..2327
                     /locus_tag="PLW16_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPB71153.1"
                     /translation="MKKKSQGLTLGRRVRAGREKLGLSLADLAHESGYTVDVLRDLEE
                     GKIVPPVSLLLQLSRVLKLDMEDGEAEEDLKTTRKRTRSHKKRVDSYAYNPLTKPAKD
                     KHLRAYHVTIEANTEHKGVEYHHEGEEFVYVIKGGLVIQVGDNVSTLAKGESLHFNSS
                     LHHKLSNPTAETTRLLVVIYVP"
     gene            2341..3480
                     /locus_tag="PLW16_09345"
     CDS             2341..3480
                     /locus_tag="PLW16_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB71154.1"
                     /translation="MSFQLTSEQEMIRLMAREFARKELEPFAHAWDAQETFPLEALRK
                     MGGLGLLGMMVPAEYGGAGVGAVSYSLALQEIAYACASTAVTMSVTNLSQDPLLKFGT
                     EAQREKYLVPLAGGEIIGAFAITEPGAGSDPGSMVMRAEDRGNHYRVNGTKVFITNGG
                     YAGSIILVARTGAEKSGRGLTAFIVEPGLPGFSVGRKEDKMGLRASNTVELIFEDCLI
                     PKENLLAKEGHGFRIAMTALDSGRIGIASQSLGIARACLDEAIQYAKSRRQFGRAIGS
                     FQALQWMIADTATEIEAAHWLTLNAADRKDQGLPFTREASMAKLFASEMANRAAYRAL
                     QIHGGYGYMKEYKIERLYRDVRVTAIYEGTSEVQRIVIARESMGA"
     gene            3527..4732
                     /locus_tag="PLW16_09350"
     CDS             3527..4732
                     /locus_tag="PLW16_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71155.1"
                     /translation="MIFNSLDTDRIAIIGPGRLGTAFAYKFDRDNKKVAIYYHDGDLC
                     RTINRERVNPKHLTQDLADRLGGMDRVPRFSPRVYATHDLERIVEGNDFIFLSVTMNR
                     LPELLNYLTPLIERKTGDTCLISPIKGLTADELTKELITPSQLINNNLYNLKYKYQVV
                     CIGGPFFDIDVALGNPVCVTVAGPRRISRLIRDELLRFNRRELNSYYNFDIVGVEACG
                     SLKNIVANLMGLAQSLSLGDSTPGTIFARSGVEIRSLSKLLGGSFQAFHSQAGVGDMY
                     VTVSSPASKNFRYGRYFYEQYMGNPIETNMKVLARIDGTPEGPNTIRNVHKYLEKKNM
                     YSPLFNCAYKIFNEGNNKKDIRELVIQACQFDKRTKEFIGPVSRVLYNLFPHFWYRRH
                     KGLMSRFEP"
     gene            5074..6435
                     /locus_tag="PLW16_09355"
     CDS             5074..6435
                     /locus_tag="PLW16_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPB71156.1"
                     /translation="MDETKVVLSDHEIPRQWYNIQADLPTPLPPPLHPGTGQPVGPED
                     LAPIFPMNIIAQDVSQERWIDIPDEVLGKYLLWRPSPLYRARNFEKLLGCPVKIYYKH
                     EGVSPAGSHKPNAAIPMAYYNREFGIKRLTTETGAGQWGSALAMACQMFGLACRVYMV
                     RVSFEQKPYRRMLMNTWGAECIPSPSSLTVSGRDFLAAHPDSPGSLGVAISEAIEDAV
                     TSENTRYSLGSVLNHVLMYQTIIGLEAEKQLEKIGVYPDVVLGCAGGGSNFAGVSFPF
                     VRDKIHGKDVRIVAAEPTSCPSITRAPYAYDFGDLAQKTPLLAMHTLGHDFVPAPIHA
                     GGLRYHGMAPLVSHVVRGGLVEARAYNQLETFDAGVKWARSEGYVPAPETNHVIAAVV
                     DEARRAKDEGKEKVILFNWSGHGLIDLTAYDAYLSGKLTQYELPEEEIERAIRTTAAF
                     PKP"
     gene            6580..6849
                     /locus_tag="PLW16_09360"
     CDS             6580..6849
                     /locus_tag="PLW16_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71157.1"
                     /translation="MTEPNEARQIRCPRLGHEIAFSYCEREGGDLPCPRIVRCWQSFL
                     PVEAYLKRTLSPDAWERFSSPTPGDKVTSLLEMVERAKGVRKERL"
     gene            6846..>7224
                     /locus_tag="PLW16_09365"
     CDS             6846..>7224
                     /locus_tag="PLW16_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB71158.1"
                     /translation="MTLPVSDNDPRPLAERMRPRTLDEFVGQSHLLGEGSLLRRAIAE
                     DRLFSIIFWGGPGCGKTTLARILAGETRSHFVSFSAVLSGVKEIRAVVEGAERRQREA
                     RLTTVLFVDEIHRFNKAQQDAFLP"
BASE COUNT         1313 a         2323 c         2405 g         1183 t
ORIGIN      
        1 ccctggcccg gaggctgctg gggccccggg cgatcatcgg cgtgtccgtc gaaacctggg
       61 aagacgtgga acaggcccag gcgctggact gcgactacct gggcgtcagc cccgtttttg
      121 cgacgccgac gaaaacggat acccgcgggg cgtgggggct cgaggggctg gcgaagatcc
      181 gggccttttc gcggcatccc ctcgtggcga tcggcggcat caacgccgcc aatgcggaag
      241 cggtctgccg ggccggggcg gatagcttgg ccgtcgtctc ggcgatctgc gcggcggccg
      301 atccgttcca ggcctcgcag gggctggcag cggtgattca cagggtccgg gaggaacggg
      361 gcgtgcgatg aagacgctcg tcgttttcga ctattccggc accctgagcc tggcggcggt
      421 ggccttcggc cgtccggacc gtctgaggga gcagctcgac gcgtccgggc tggcggcgct
      481 cgggattgcg agccccgggg tcttctggga tgaggtcgtc ggcccgacct ggacggaggg
      541 gagcacgacg ccggtcggct accggggcgt catggcgcgg cggatccgcg aacggtgcgg
      601 cgtccgcccg ggagacccgg cggacgaccg gattcgtgac gccgtttccc ggttcgtcgg
      661 cgcctatctg gggcagtccc ggatcgatct tcgctggcag cccctcctga acgccctaac
      721 ctcgaatccg gacgtgcaac tcctcgtcgc cacggaccac tatgccgagg cgacggatta
      781 tatcgtgggc tatctccgcg acggggggct caccgcccgg gcgatccggg acgagccgga
      841 agcgccggcg gcgcctgccc ggatcctggt cgccaattcc gccgatctcg gcgccccgaa
      901 ggcggcgcgc gccttctggg agcagtcccg tcgcctcctc cccgcgcgga tggatcgggt
      961 catcctcatc gacgacttcg ggttcaacga acaggcggga gacagctacg gcgcccggca
     1021 gaaggtggcg gagcggatgg cgaaaaccgc cttcctcctg gaggaggtct tcgacggggc
     1081 ggtggaggtc gtgcccttta tgatcgaacc gggcctgccg gccggtgcgg cgggtgccgt
     1141 gatccgcgaa acggacggac aggtccgggc cctcctgtcc gggacggcgg gcggctctcc
     1201 ctcgccatcg gcccggcaga cggagaaccc tgcgcccggc ggaagcgggc tgcagccccc
     1261 ccgcgaacct ccggcctgca aacggaaacc cgacctgacc tatccctgcc cctgggtata
     1321 caaggtcgtc ggcgcggacc cggacgcgct ccgccaggcc atcgccgccg tcgtcggtcc
     1381 ccgccgccac acggtgacgc cttcccgcac gagcgccaac gggaaatatc acagtctgaa
     1441 cctggccctg gaggtgcggg acgaggcgga gcgcctgacc ctctacgacg gtctccggcg
     1501 tcagccggcc gtccggatcg tcctgtaaag accgcctccg tttcaccgca ggccgtcgga
     1561 ggaatgtttc cgttttccgg acgatccgaa cggaccccgc ggcggggcgc tttgtccttg
     1621 acacgctgcc ggggcttccc tatactccac ccgatttttc cggccccgcc cgtcaccggg
     1681 ggcgttgcgc tccaacccat ggggggctgt ctccgtgaag gtcgcggggt cggagcagaa
     1741 cccggcttgt ccggggaaca atgagggagg tgcccgtggt gaagaaaaag tcacaggggc
     1801 tgaccctggg gcggcgcgtc cgggccggcc gggagaagtt gggcctgagc ctcgcagacc
     1861 tggcccatga gagcggttac acggtcgatg tcctgcggga tctcgaggag gggaaaatcg
     1921 tccctcccgt ctccctgctg cttcagctga gccgggtgct gaaactcgac atggaggacg
     1981 gggaggcgga ggaggacctc aagaccaccc ggaaacgaac ccggagccac aagaagcggg
     2041 tggattccta cgcctacaac cccctgacca agccggccaa ggacaagcac ctgcgggcct
     2101 accacgtcac gatcgaggcc aacacggagc acaagggggt cgagtaccac cacgaggggg
     2161 aggagttcgt gtacgtcatc aagggcggac tcgtcatcca ggtgggcgac aatgtctcga
     2221 cgctggcgaa gggggagagc ctccacttca attcctccct ccatcacaag ctgagcaatc
     2281 cgaccgcgga gacgaccagg ctgctcgtcg tcatctacgt gccgtaaggg gggagggggg
     2341 atgtcttttc agctcaccag cgaacaggaa atgatccgcc tgatggcccg ggagttcgcc
     2401 cgcaaggagc tggagccctt cgcccacgcg tgggacgcac aggagacgtt tcccctggag
     2461 gcgctccgca agatgggggg gctggggctg ctcgggatga tggtgccggc ggagtacggc
     2521 ggggcaggcg tcggggcggt cagctattcc ctggcactgc aggagatcgc ctatgcctgc
     2581 gcctccacgg cggtgaccat gtcggtgacg aacctctccc aggatcccct gctcaaattc
     2641 gggacggagg cgcagcggga gaaatacctc gtcccgctgg cgggcgggga gatcatcggc
     2701 gcctttgcca tcacggagcc cggtgcgggc tccgatccgg gcagcatggt catgcgcgcg
     2761 gaggaccggg gcaatcatta tcgggtcaac ggcacgaagg tcttcatcac gaacgggggc
     2821 tacgcggggt caatcatcct ggtggcccgg acgggcgcgg agaagtcggg ccgggggcta
     2881 acggccttca tcgtcgaacc gggcctgccg gggttttcgg tgggccggaa ggaagacaag
     2941 atggggctcc gcgcctccaa cacggtggag ctgatcttcg aagactgtct cattccgaag
     3001 gaaaacctcc tggcaaagga gggccacggc ttccggatcg cgatgacggc cctagacagc
     3061 gggcgcatcg gcatcgcgtc ccagtccctc ggcatcgctc gggcctgtct ggacgaagcg
     3121 atccagtacg caaaatcgcg ccgtcagttc ggtcgggcga tcgggtcgtt ccaggccctc
     3181 cagtggatga tcgcggacac ggcgacggag atcgaggccg cccactggct gaccctgaac
     3241 gcggcggatc gcaaggacca ggggcttccg tttacccggg aggcgtcgat ggcgaagctt
     3301 ttcgcctcgg agatggccaa ccgggcggcc taccgtgccc tgcagatcca cggcgggtac
     3361 ggctacatga aggaatacaa gatcgagcga ctgtaccggg acgtgcgcgt caccgcgatc
     3421 tacgagggga cctcggaggt ccagcgcatc gtcatcgcgc gggagtccat gggggcctga
     3481 gcggcgtcgc cccgctccgg caaatcggcg ccagtggcag gggatcatga ttttcaacag
     3541 tctggacacg gacagaatcg ccatcatcgg ccccgggcgt ctcgggacgg ccttcgccta
     3601 caagttcgac cgcgacaaca agaaggtcgc gatctattac catgacggcg acctctgccg
     3661 caccatcaac cgggaacgcg tcaatccgaa acacctgacc caggacctcg ccgaccgtct
     3721 gggcgggatg gaccgggttc cgcgattctc cccccgggtc tacgcgaccc acgatctgga
     3781 gcggatcgtc gagggcaatg acttcatttt tctgtccgtg acgatgaacc gcctgccgga
     3841 gctcctcaat tacctgaccc ccctgatcga gcgcaagacg ggcgacacgt gcctgatctc
     3901 gcccatcaag gggctcaccg ccgacgagct gaccaaggag ctcatcaccc cgtcccagct
     3961 catcaacaac aacctctaca acctcaagta caagtatcag gtggtctgca tcggcgggcc
     4021 gttcttcgac atcgacgtgg ccctggggaa tccggtctgc gtcacggtgg ccggaccacg
     4081 gcgcatctcc cgtctcatcc gggacgaact gctccgcttc aaccggcgtg aactgaactc
     4141 ctactacaat ttcgacatcg tgggggtaga ggcctgcgga tcactcaaga acatcgtggc
     4201 caacctcatg gggctggcgc agtcgctgtc gctgggcgat tccacgcccg gcacgatctt
     4261 cgcccgttcc ggcgtggaga tccgctcgct gtcgaagctc ctgggcggga gcttccaggc
     4321 cttccacagc caggcgggcg tcggcgacat gtacgtgacc gtatcctctc ccgccagcaa
     4381 gaacttccgg tacggccggt atttctacga gcagtacatg ggcaacccca tcgagaccaa
     4441 catgaaggtc ctcgcccgga tcgacgggac gcccgaaggg ccgaacacga tccggaacgt
     4501 ccacaagtac ctggagaaga agaacatgta cagccccctg ttcaactgcg cctacaagat
     4561 cttcaacgag gggaacaaca aaaaggatat ccgggaactc gtcatccagg cctgccagtt
     4621 cgacaagcgg acgaaggagt tcatcggccc ggtctcccgg gtcctgtata acctcttccc
     4681 ccacttctgg taccggcggc acaaggggct tatgtcccgt ttcgaaccct gaggcggagg
     4741 gaaggaccgg tgtcgaaaat ccggccacaa aaacacctgc aaaaaaatac ttgccctcgg
     4801 gttttttttt tgatagggaa ccttcccacg attgagagga aagaggttcg gttgatggat
     4861 cggcggtctc aaaacaggtt gttctggtgg tggcagacgt aacgtctgcc cgcgagcgct
     4921 cgccggtatc gagcgggccg tgggcgggag aggacccccc atggccgagc acggagagaa
     4981 agaatcaaaa gccacggggc gcgccccgtg gctttttttg cgcggtgcgt tcattcaggg
     5041 acgcgcctat ttcaggaaaa aggagagcag atcatggacg agacgaaggt agtgctgagc
     5101 gatcatgaaa ttccccggca atggtacaac atccaggcgg atcttccgac cccgttgccg
     5161 ccgcccctcc atcccgggac ggggcagccg gtcgggccgg aggatctggc cccgatcttc
     5221 ccgatgaaca tcatcgcgca ggacgtgagc caggagcgct ggatcgacat tccggacgag
     5281 gtgctgggga aataccttct gtggcggccg tctcccctct accgagcccg caacttcgaa
     5341 aagctcctcg gctgcccggt gaagatctac tacaagcatg agggggtgag tccggccgga
     5401 tcccacaagc cgaacgccgc gatccccatg gcgtactaca accgcgagtt cgggatcaaa
     5461 cgcctgacga cggaaacggg cgccgggcag tgggggagcg ccctggccat ggcctgccag
     5521 atgttcggtc tggcctgccg ggtgtacatg gtgcgggtca gcttcgaaca gaagccgtac
     5581 cggcggatgc tgatgaacac gtggggcgcc gagtgcatcc caagtccgag cagcctgacc
     5641 gtatcgggtc gcgactttct ggccgcgcac ccggattcgc cgggaagcct gggagtcgcc
     5701 atcagcgagg ccatcgagga tgcggtaacc tccgagaaca cccggtactc cctgggcagc
     5761 gtgctcaacc atgtcctgat gtaccagacg atcatcggtc tcgaggccga gaaacagctg
     5821 gagaagatcg gcgtctatcc cgatgtcgtg ctgggctgcg ccggcggcgg cagcaacttt
     5881 gccggcgtct cgttcccctt cgtccgggac aagatccacg ggaaagacgt tcggattgtg
     5941 gcggccgagc ccacctcctg tccgtcgatc acccgggcgc cctatgccta tgacttcggc
     6001 gatctggcac agaagacgcc gcttttggcc atgcacacgc tggggcatga tttcgtgccc
     6061 gcgcccatcc acgcgggcgg actgcgctat cacggcatgg cgccgctcgt cagccacgtc
     6121 gtccgtgggg gcctggtcga ggcgcgggcg tacaaccagc tggagacctt cgacgccggc
     6181 gtgaaatggg cgcggtcgga aggctatgtc ccggcgccgg agacgaacca cgtgatcgcc
     6241 gcggtcgtcg acgaggcgcg gcgggccaag gatgagggga aggagaaggt tatcctgttc
     6301 aactggagcg gccacggtct gatcgatttg accgcctacg acgcctacct atccggaaag
     6361 ctcacgcaat acgagctgcc cgaagaggag atagagcggg cgatccggac gacggcggcc
     6421 ttccccaagc cgtagatcca cggacggcat ccccgggcgg ggtggcgatt gtgccggggg
     6481 cgaaaacgcc accccgttct ttttccctgc cgcggggaag aaatcgcttg acggcggagg
     6541 ggagatcggc tttcaataga ctccgaagaa ggaaggacca tgacggaacc aaacgaggcg
     6601 cggcagatcc gttgtccgcg cctcgggcac gagattgcgt tttcctattg cgagcgggaa
     6661 ggcggagacc tgccctgtcc gcggatcgtc cgctgctggc agtccttcct gcccgtcgag
     6721 gcctatctga aacggaccct ttccccggac gcctgggagc ggttttcctc accgacgccc
     6781 ggggacaagg tgacgtccct gctcgagatg gtcgagcggg cgaagggggt ccggaaggaa
     6841 cgtctttgac cctccccgtc tccgacaacg atccgcgtcc cctcgccgag cgcatgcggc
     6901 cgcgcaccct cgacgaattc gtaggtcagt ctcacctgct gggtgaggga tcgctcctgc
     6961 ggcgggccat tgccgaagac cgcctcttct ccatcatctt ctggggcggt cccggctgcg
     7021 ggaagaccac gctggccagg atcctggccg gcgaaacccg gtcccatttc gtcagcttct
     7081 ccgcggtcct ctccggtgtc aaggagatcc gggcggtggt cgagggggcg gagcgccggc
     7141 agcgggaagc gcgcctcacg accgtcctgt tcgtcgacga gatccaccgc ttcaacaagg
     7201 cccagcagga cgcctttctc cccc
//
LOCUS       DAOOLJ010000080         6772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_13376_length_6772_cov_12.056722, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000080 DAOOLJ010000000
VERSION     DAOOLJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_6772_cov_12.056722"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="PLW16_09370"
     CDS             <1..1491
                     /locus_tag="PLW16_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB71159.1"
                     /translation="STHIAVIDIGRTNKKLLLYDLKLNPVDGAYATIADIPHGNENHE
                     PIDATAAWFLNALADMAASHRIANITITTYGGAFVCLDEQGRVALPPLSGSTDPGEDV
                     QASFDALIGDPRRIQRRMATPRMPFLSVMARGIHYVKERYPELFARTRTLINLPQYYG
                     YLLTGQTGNERTYMGTHTALWDFERMDWSEVRERLGVEALIPPTLSRAWEVLGTLTPQ
                     VARKTGLAPEVRVTMGIHDSNAAMVPFLIKQPEDFMLVSTGSMCVVMHPEPGVRFREE
                     DFGEMVYYNLNAFDGVFKTALFVAGLEFDIYMGLLGGRHGRQDPPDIDPELLADILGR
                     REEFILPAIVPFGMYLNSPARLVEGDQVYPLGDVFSGKGPRFLDDYERSCALLTLSMA
                     LHTRKAMERAGHAPGMPVIIEGGFSHNALYTRLIASLAPESPVMLTGLKEASAFGAAL
                     LGLAAHEGVSPHRLAERFTLTTTRVPPFELPNLRAYVETFERRTQG"
     gene            complement(1524..2015)
                     /locus_tag="PLW16_09375"
     CDS             complement(1524..2015)
                     /locus_tag="PLW16_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB71160.1"
                     /translation="MKALVTYYSQTGNTEKVARSIYDSLPADKELLPVHDVRDVTGYD
                     VVFCGFPVQAHSVPVKVHGLLKNLPDGQKVAFFCTHGSLRGGSLPKQAIESAIGMVPR
                     AKVLGTFGCRGKVDPQIIALLRAKPEHRQWAEEAVGADAHPDEADLSDARAFAAGILA
                     KVR"
     gene            complement(2308..3288)
                     /locus_tag="PLW16_09380"
     CDS             complement(2308..3288)
                     /locus_tag="PLW16_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB71161.1"
                     /translation="MIIDSHVHILEEGWRPEAFLQGVARIGADISGIDKDVIRNSIFV
                     TFWDGEGKKRIAAMERDGIDICIIHPIDFGLADRCSEPVVPYQLQNETMWNIARKYEG
                     RMYCSVSVDPRRKDAVALLERGVSGFGAVALKLHPGSGFYPNDKICYPLYEKAAELNV
                     PVVLHTGPIATPLKSKFCLPMFVDELAADFPQTNFCMAHMGFRLWPEAIGIAEVNTNV
                     FLDISGWQGESKHPRRFYGILRTVLDTISSEKVMWATDGPMYSQIVPDREFAQVIKDA
                     GKNGAAFGYAFSEIEINNLLGGSAARFFNLKLTPSGTDTAPGQTQDSRNP"
     gene            complement(3306..4628)
                     /locus_tag="PLW16_09385"
     CDS             complement(3306..4628)
                     /locus_tag="PLW16_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB71162.1"
                     /translation="MTDQTQNPHDPAAGAPLPISRGKAYYAYVLLFLLYMFDYIDRQV
                     VVSVFPFLKADWELTDLQCGMLVSAVYWSIVIFTLPVSILIDRWSRKNSIAIMAIFWS
                     VASVACAFTRNFTQLFAARTAIGIGEAGYAPGGATLISTTFPKAKRALMLGIWNAAIP
                     LGIAIGTALGAVIAERWGWRHAFGLVAIPGFFIALLFFRVKDYKTVSLADTLGQAESG
                     AGDKVGVGTVARDILGNRTLLLIYMGFAASVFVTTSLSTWLPSYFQRTYHIPAGQAGF
                     MGGMVMLMSIIGAPLGGFLADLWMKKTPNGRPYFIALSSLITSIALFLSFAVLQGTVQ
                     YVMLIFAGITAVAFVSGAMAIIQDVSNPGLRATYFSLNVVIQNLLGSSLGPIVIGSIS
                     DHYTLGTAMAVMPVFSLAAAAAFISASRFYSRAAGRVEAIEAQIQAAP"
     gene            5383..6693
                     /locus_tag="PLW16_09390"
     CDS             5383..6693
                     /locus_tag="PLW16_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HPB71163.1"
                     /translation="MRREYGFGVLCCVLGFLVGICFVGQVEGRIYYRCGNGHDEPTCE
                     DHGGYLCKKDNKVGKKLTDCLKFDGAVWEDRPPFCGDGWIEGIEECDDGQWNSDSLPN
                     ACRTDCRRAHCGDEVHDRGEDCDNGRRNSDIVPNACRTSCRFPSCGDGLHDFDYGEAC
                     DDGNQDESDGCRSDCRTCTRLTGNVFVNAETFFLCKGDYSVASYPDAGAIIVQNENYG
                     IVVDCAGATLHGSGDGAGIFVRNTSATIRNCVITGYAAGIRAVNSHVVIEASNRLTGN
                     TRGIVLENSTRSDSTKAVTPMVGMPKGAAKGMRPETSPDGGKAESAKIASPPIRARTG
                     LSAAPPSPGARLPLTVAKPVPAVPPSAKVVAEGKRVFLNLNVAVARAEVYAGNRLVGR
                     LPSGNRLEITNHVPKAVRGELILHYFDARGTKTVQAVKIGSDRK"
BASE COUNT         1350 a         1958 c         2119 g         1345 t
ORIGIN      
        1 tcgacgcaca tcgcggtcat cgacatcggc cggaccaaca agaaactgct cctctacgat
       61 ctgaagctca acccggtaga cggcgcctat gccacgatcg ccgatattcc ccacgggaac
      121 gagaatcacg agccgatcga cgcaacggcc gcctggttct tgaacgcgct cgcggacatg
      181 gccgcgagcc accgcatcgc caacatcacg atcacgacgt acggcggcgc atttgtctgc
      241 ctggacgaac aggggcgcgt ggcgctgccg cccctctccg gctccaccga tccgggcgag
      301 gacgttcagg cgtccttcga cgcgctcatc ggcgatcctc gccggatcca gcgtcgaatg
      361 gccacgcccc ggatgccgtt tctcagcgtc atggcgcgcg ggatccacta tgtcaaggag
      421 cgctatccgg agctgttcgc ccggacccgg accctgatca acctgcccca gtattacggc
      481 tatctgctca ccggtcaaac gggaaacgag agaacctaca tgggaaccca cacggccctg
      541 tgggacttcg agcggatgga ctggtccgag gtgcgcgagc ggctgggggt ggaggcgctg
      601 attcctccga cgctttcccg ggcatgggaa gttctgggaa cgttgactcc ccaggtggcc
      661 cggaaaaccg ggcttgcccc cgaggtccgc gtgacgatgg gcattcacga ctccaacgcc
      721 gccatggtgc cctttctgat caagcagccg gaggatttca tgctggtgtc caccgggtcc
      781 atgtgcgtgg tcatgcatcc cgaacccggc gtgagattcc gggaggagga tttcggcgag
      841 atggtttact acaacctgaa cgccttcgac ggcgtcttca agacggcgct cttcgtggcc
      901 ggactggagt tcgacattta tatgggactt ttgggggggc gccacggccg gcaggacccg
      961 cccgacatcg acccggagct gctcgcggac atcctcggcc gccgggagga attcatcctg
     1021 ccggcgatcg tccccttcgg catgtacctg aactccccgg cccgtctcgt cgaaggggat
     1081 caggtttatc ccctgggaga tgtcttttcg ggcaaaggcc cgcgctttct ggacgattac
     1141 gagcggtcct gcgccctgct gacactcagc atggccctcc atacccgcaa ggccatggag
     1201 cgtgcgggcc atgcgccggg catgcccgtc atcatcgagg ggggattctc ccacaacgcc
     1261 ctgtatacgc gcttgatcgc gtcgctcgct ccggaatccc ccgtgatgct caccggcctg
     1321 aaagaggcct ccgcctttgg cgccgccctg ctgggattgg ccgcccacga gggcgtgtct
     1381 ccgcatcggc tcgcggagcg cttcacgctg acgaccaccc gggtgccgcc tttcgagttg
     1441 ccgaacttgc gggcctacgt cgaaactttt gaacggcgga cgcaaggctg agcgggacga
     1501 tattatgctt ctcctgttgc cggttatcgg accttggcga gaatgccggc ggcgaatgcc
     1561 cgcgcgtcgc tgaggtccgc ctcgtcggga tgggcatccg ctcccacggc ctcttccgcc
     1621 cactgccgat gctccggctt tgcccgcagc aaggcgatga tctgggggtc caccttgccc
     1681 cggcaaccga aagtccccag gaccttggcc cggggcacca tgccgatggc gctctcgatg
     1741 gcctgcttcg gaagcgaccc gccgcgcagc gatccatggg tgcagaagaa agcaaccttc
     1801 tgcccgtccg gcagattctt gagaagaccg tgcaccttga caggcacgct gtgcgcctgc
     1861 acgggaaacc cgcaaaacac gacgtcatag cccgtcacgt cccggacgtc gtgaacggga
     1921 agaagttcct tgtccgccgg cagactgtcg taaatggatc gagccacctt ttccgtatta
     1981 cccgtctggg agtaatacgt gaccaatgcc ttcatctttc ctccttctga gtctgtgatt
     2041 tacggggggt cgtcccccga tgcgccgggc gcgactcgga agtcaactcg aacagagagc
     2101 cgccctcttc ggttccgtcg ctcccggaag ccccacactt cataggggaa ttccccgccc
     2161 ttgtcaagat gggccacgga cacctccacc ccggtggtct gaagggtctt ttcggccatt
     2221 cttcgcgccg gtcgctgggg gtgatgcctc ctggctgacc gagacaaagc gcgggggggg
     2281 ggagcgcgtc tctttttcga tccctcatca agggtttcga gaatcttgcg tctgccccgg
     2341 agcagtatcg gttccggagg gggtgagttt caggttgaag aaacgcgcgg cgctgccgcc
     2401 caacaggttg ttgatctcga tctccgagaa agcataaccg aaggccgccc cgttcttgcc
     2461 ggcatccttg atgacctgcg cgaactccct gtcggggaca atctggctgt acatgggtcc
     2521 gtccgtggcc cacattacct tttcggaaga gattgtatcc aggaccgtcc tcagaatccc
     2581 gtagaaccgc cggggatgtt tcgattcgcc ctgccagccg gagatgtcca gaaacacgtt
     2641 ggtgttaacc tccgcgatgc cgatggcttc aggccatagc cggaacccca tgtgcgccat
     2701 gcagaagttg gtctgcggaa aatcggcggc caactcgtcg acgaacatgg gcagacagaa
     2761 cttacttttg agcggggtgg cgatgggacc cgtgtggagg acaaccggca cgttcaattc
     2821 cgccgccttc tcgtaaaggg ggtagcagat tttgtcgtta gggtaaaaac ccgaacccgg
     2881 gtgcagcttc agggcaaccg cgccgaatcc ggacacgccc cgctccagca gcgccactgc
     2941 gtcctttcgg cggggatcga cgctgacgga gcagtacatc cggccctcgt atttccgggc
     3001 gatgttccac atggtctcgt tttgaagctg gtaggggacg accggctccg agcagcggtc
     3061 ggcgaggccg aaatcaatag ggtggatgat gcagatgtcg atgccgtccc gctccatggc
     3121 ggcgatgcgc tttttgcctt caccatccca gaaggtgacg aagatgctgt tgcggataac
     3181 atctttatcg ataccgctga tgtcggcgcc gatcctggcg accccctgca gaaacgcttc
     3241 gggacgccag ccctcttcga gaatatggac gtgggaatcg atgatcatga atcacctccg
     3301 ggtttctatg gggcagcctg gatctgtgcc tcgatggcct ccaccctccc ggccgctcgg
     3361 ctgtagaagc gtgatgcgga gatgaaggcg gcggcggcgg cgaggctgaa gaccggcatg
     3421 acggccatgg ccgtgcccag ggtatagtga tcggaaatcg aaccgatgac gatggggccg
     3481 agagagcttc ccagcaggtt ctgaatgacg acgttgaggc tgaaataggt tgcccgcaga
     3541 ccggggttgg agacgtcctg gatgatcgcc atggcgcccg aaacgaaggc cacggcggtg
     3601 atgcccgcaa agatcagcat gacgtattgg acggttccct gaaggacggc gaaggacagg
     3661 aagagggcga ttgaggtgat caaggaggac agggcgatga aatagggccg gccgttcggt
     3721 gtcttcttca tccagagatc ggcgagaaag ccgccgaggg gagcgccgat gatggacatg
     3781 agcatgacca tgccgcccat gaagccggcc tgtccggcgg ggatgtggta cgtccgctgg
     3841 aaataggagg gcaaccaggt gctgagggac gtggtgacga agacgctggc ggcgaagccc
     3901 atgtagatga gtaagagcgt cctgtttccc aggatgtctc tggcaaccgt cccgacgccg
     3961 accttgtccc cagccccgct ttccgcttgg cccagcgtgt cggccaggga aacggtttta
     4021 taatccttga cccggaagaa caggagcgcg atgaagaacc cgggaatggc gacgaggccg
     4081 aaggcatgcc gccagcccca gcgctccgcg atcacggccc ccagggcggt tccgatcgcg
     4141 atccccagcg gaatcgcggc attccagatg ccgagcatca gtgctcgttt cgccttgggg
     4201 aaggtcgtgg agatgagcgt ggcgccgccc ggcgcgtacc ccgcctcgcc gatgccgatc
     4261 gccgtccggg cggcgaagag ctgggtgaag ttccgggtga acgcgcaggc gacggaggca
     4321 acgctccaga agatcgccat gatggcgatg ctgtttttcc ggctccagcg gtcgatcagg
     4381 atggaaacgg gcagggtgaa gatgacgatg gaccagtaaa cggcggagac gagcatgccg
     4441 cactgtaggt cggtgagttc ccagtcggcc ttgaggaacg gaaagaccga gaccacgacc
     4501 tgccgatcga tgtaatcgaa catgtacagg aggaagagca ggacgtaggc ataataggcc
     4561 ttcccccggg aaatgggcag gggcgccccg gcggcgggat cgtgggggtt ctgcgtttgg
     4621 tcggtcatgg tcaggtttgc ttccccggtg atgagagtta tctcaaatcc ctcgtcgcgt
     4681 caagatgaac ctgcgggagg gagcggccgc gcccacggag agggtgtagc agcgcatgaa
     4741 gcccggcgga ttgtaacatg cccggagccg cagattagaa catcaattgc ccgtcggatg
     4801 cgccgtgtga cttgtattga tctcgataaa ctctgccgcc gaacttcttt tcttgatctt
     4861 cttgttgctg gcctggccgt cgcggaagct cgccgtcgtc aaattgtctg atgaggcacg
     4921 tttccagatt tctggacatt tcttgcgtca gttttgtcct gatgaagata attgcggtct
     4981 gcccctggcg tcgttcgtgt cctttttccc gaactcatga gttacagaga cggaggagat
     5041 acagaggacg caacgcccgt cctttctcac tttccaggtg gtgccggggg gctcggggaa
     5101 gcattctttt ttgagttcat ccgattcttg ggtatttcct gaaagaaagc gagcggattt
     5161 taaacggcca tccatgcttt cgaagcatta tcgtggaaaa atctgcttga actatttatg
     5221 gtttaggtgc acaaaaacag taacgtctat cggtaaccta catcataacc cctttcccct
     5281 aattgaatgc cctgcgttca aaagaaaaag gatgttgtgc aaaaaacgct gcacatcagt
     5341 atgatcagcc gatccggcca tgaccgatcg ggggaggttg ccatgagaag agaatacggg
     5401 ttcggtgtgc tatgttgtgt cctgggtttt ctcgtcggta tctgctttgt cggtcaggta
     5461 gagggacgca tctactatag gtgtggtaac ggccatgatg aaccgacctg cgaggatcac
     5521 ggtggctatc tatgcaagaa agacaataag gtgggcaaga aactcaccga ttgcctgaag
     5581 ttcgacggag cggtctggga agatagaccg ccattttgtg gcgatggttg gattgagggg
     5641 atcgaagaat gcgacgacgg acagtggaac agtgattccc tcccgaatgc ctgccggacg
     5701 gattgccgac gcgcccattg cggcgacgag gtgcacgaca ggggggaaga ctgcgataat
     5761 ggtcgcagaa attcggatat cgtgcccaat gcctgtcgca caagctgccg gttcccaagt
     5821 tgcggggacg gcctgcatga tttcgattac ggcgaggcgt gcgacgacgg caaccaggac
     5881 gaatccgacg gttgccggtc ggattgcagg acctgcaccc ggttgaccgg aaacgtcttt
     5941 gtcaatgccg aaacgttttt tctctgcaaa ggcgactact ctgtcgccag ctacccggat
     6001 gccggggcga tcatcgtcca aaatgaaaac tatgggatcg tggtcgactg cgccggggca
     6061 accctgcatg gttcaggaga tggagccggc atctttgttc ggaatacgtc tgcgaccatc
     6121 cgcaactgcg tgattaccgg ctatgcggcc gggatccggg ctgtcaattc acatgtggtg
     6181 atcgaggcta gcaaccgttt gaccggcaac acccggggca tcgtgctgga gaactcgact
     6241 cggtctgatt caacaaaggc cgtgacgccc atggtgggca tgcccaaggg cgccgcgaag
     6301 gggatgcgtc cggaaacctc ccctgacggt ggtaaagcgg aatcggcaaa gattgcctcc
     6361 cctccgatcc gagcgcggac cgggctatcg gctgcccctc cctctccagg tgctagactg
     6421 ccattgaccg ttgccaaacc ggtgccggcg gtgcccccca gcgcgaaggt cgttgcggaa
     6481 ggaaaacggg ttttcctcaa tcttaacgtg gccgtggccc gggcggaagt ctatgccggg
     6541 aacaggctcg tgggtcggct cccgagtgga aaccgtctcg aaatcaccaa ccacgttccg
     6601 aaagctgtgc ggggagaact gatactgcac tattttgatg cgagggggac caagaccgtg
     6661 caagctgtaa aaatcggttc ggacaggaaa taaactgtcg taagatggtg cgtagttgac
     6721 agcttgtgta aagatggcta cgaaggaaaa cttcctgtag tcttcttttt tg
//
LOCUS       DAOOLJ010000081         6192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_15091_length_6192_cov_15.786215, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000081 DAOOLJ010000000
VERSION     DAOOLJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15091_length_6192_cov_15.786215"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="aroF"
                     /locus_tag="PLW16_09395"
     CDS             <1..920
                     /gene="aroF"
                     /locus_tag="PLW16_09395"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPB71164.1"
                     /translation="IIGAVGDERGKGQLKSVENLPGVEKVVPILKPYKLASREFSEAD
                     TVIPVGDMKIGGTSFVVMAGPCSVESEEQLMESAYIVRKGHAQILRGGAFKPRTSPYS
                     FQGMEEEGLKLLKKASERTGLPVVTEVMNTTEVDLVASYADIVQIGARNVQNFALLKR
                     VGQIRKPVLLKRGMMTTIEELLMSAEYILDSGNDQVILCERGIRTFETATRNTLDLSA
                     VPVLKGLTHLPVIVDPSHAAGHWRYVIPLARAAVAVGADGIIIEVHPEPEKAVSDGAQ
                     SLKPEKFYRMMEELRVIAGVMGKEMGVPA"
     gene            917..2011
                     /gene="aroB"
                     /locus_tag="PLW16_09400"
     CDS             917..2011
                     /gene="aroB"
                     /locus_tag="PLW16_09400"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPB71165.1"
                     /translation="MKKIRVNLDRKSADAHEIFIGRGIIDRMGLLMAKNNWASRYVLV
                     TDSRVAELHGDRVLATLRGMNLAVDMIQFPAGEASKTVRTCLDLVERLLALGADRKSA
                     LIALGGGVVGDLTGLAASLFMRGIPYLQAPTTLLAQVDSAIGGKTGVNLPAAKNMLGT
                     FYQPRGVFIDLAFLDTLPEREFANGLAEIVKYGVIEDPELLKHLADEAEAIRGRDGAV
                     LERLVARSCRIKKGIVELDETEKGLRRILNFGHTMGHAVEAATDYGMAHGEAVAVGMV
                     AAARLSVALDHLSSDEGERIEAVLRAIGLPVRIPGNVSAERLLDGLRMDKKKEGVDVH
                     FVLLKKIGIPFVNGGVPEALLRKTIEDLQS"
     gene            2008..3087
                     /gene="pheA"
                     /locus_tag="PLW16_09405"
     CDS             2008..3087
                     /gene="pheA"
                     /locus_tag="PLW16_09405"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPB71166.1"
                     /translation="MKKTPLDDLRAAVKERDRELVRLLNERAKLSVEIGRVKAVQNRP
                     VYDPAQEAEVYARLEALNEGPLTGPALRDVFREILSQSRALQTPMTVAYLGPEASFSH
                     LAALSHFGRQARYVHAATIAEVFDAAEKDKGVWGIVPVENSTEGAVKRTLDRLMTTPL
                     TIRAERFLRITLCLMAQRSGRKGIRRIYSHPQALAQCRGWLDRNVPRAERLEVASTAA
                     AVRKAQEEKDAAAVGSPMAAEAYGLKVVAAGIEDSPWNTTRFFVIGRGEGEPTGRDKT
                     SILFGTPHVPGTLLNALEPLAREGVNMTRIESYPLRDRMWEYLFFVDFLGHRRDEKVR
                     RCLAEMAAKTTLIKILGSYPQGEEA"
     gene            3159..3863
                     /gene="aroD"
                     /locus_tag="PLW16_09410"
     CDS             3159..3863
                     /gene="aroD"
                     /locus_tag="PLW16_09410"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPB71167.1"
                     /translation="MRICVPVTAATNEAALALMDRVLAEADLVEIRLDAIRKPDLARL
                     LRGRRGRVLVTNRSAAEGGAFDGSEVRRVALLREAVALGAAYVDVEAATDGGLIRSLR
                     ETLRDQDGATKLVVSWHDVTGTPSARALRKKLRELMAFKADVVKLVTRAETVADAFRV
                     LELIPAAGRRGQEIVAFAMGPRGRASRLLSVLMGASWTFAALAAGAESAPGQMTVGET
                     RQSMSVLTRGSQNGRP"
     gene            3957..4760
                     /locus_tag="PLW16_09415"
     CDS             3957..4760
                     /locus_tag="PLW16_09415"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPB71168.1"
                     /translation="MDVYGVIGNPVGHSLSPLMHQAALDALGIPARYLAFAVRDLPAA
                     VQGILGLNLRGVSVTLPFKTEVMTLLDEVEADASAIGAVNTIVNDHGRLTGFNTDGTG
                     LIRDLREVMDIEGRRFAVLGAGGAARAAVFGLLQAGGIPVVLNRTAQKAEALAAAFGC
                     DFRPLAEAAGVEADCLINTTPVGMAPGVERSPLTADVLGRFRWVVDIIYTPLRTRLLR
                     EAAAVGCRTRSGLGMFIHQGAEQLRIWTGRTPPVDLMRRVVEARLIEAG"
     gene            4785..6059
                     /gene="aroA"
                     /locus_tag="PLW16_09420"
     CDS             4785..6059
                     /gene="aroA"
                     /locus_tag="PLW16_09420"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPB71169.1"
                     /translation="MEDLTEIRPLRDLDARVTLPGSKSYTQRALVIAGLAEGTSVLRD
                     PLLSEDTGVMIDALRALGAGIAREDGDLVVAGTAGKIARPEGEIRLGNNGTALRFLTA
                     VVSLGRGVFTLTGDPRLCERPIGPLLEALDALGVDARSRGDNGHPPVVVVADGLKGGQ
                     VVFRDIESSQYVSALLLSAPYASGDTRLDLAGCIPSLPYVAMTIEAMATFGQDIARPA
                     AHAFAVRGNGGYRGREYRVEGDASSASYFFLAAALTGGRVRVDNVRPRTLQGDIGLLA
                     ILEELGCHVRRGEDWVEVEGGRLAPGDVALGLGDMPDMVPTVAVLAALRPGRTIIRNA
                     AHLRLKESDRLAALASELVRVGIGARETEDGLVIDGGTPHGAVIETYNDHRIAMSFAI
                     LGLVVPGVRIVNGACVGKSFPGFWQALEGLYA"
BASE COUNT          994 a         1995 c         2237 g          966 t
ORIGIN      
        1 gcatcatcgg cgccgtcggg gacgaacgcg gcaaggggca gctcaaatcc gtcgagaacc
       61 tgcccggggt ggaaaaggtg gtcccgatcc tcaaacccta caagctcgcc agtcgcgagt
      121 tttccgaggc cgataccgtc atcccggtcg gagatatgaa gatcggcggg acgtccttcg
      181 tcgtcatggc cggtccctgt tccgtggaaa gcgaggaaca gttgatggag agcgcctaca
      241 tcgtccgcaa gggccacgcg cagatcctgc gtggcggggc cttcaagcct cggacgtcgc
      301 cctacagctt ccagggcatg gaggaagagg gcctgaagct cctgaaaaag gccagcgagc
      361 ggaccgggct gccggtggtc acggaggtca tgaatacgac ggaggtggac ctggtggcgt
      421 cgtacgccga catcgtccag atcggcgccc gcaacgtcca gaatttcgcg ctgctgaagc
      481 gggtcggcca gatccgcaag ccggtccttc tcaagcgggg catgatgacg acgatcgagg
      541 agctcctcat gtcggcggag tacatcctgg attcggggaa cgaccaggtc atcctctgcg
      601 aacgggggat ccgcaccttc gagacggcga cgcgcaacac cctggatctg agcgccgtgc
      661 cggtgctcaa gggattgacg cacctgcccg tcatcgtcga tcctagccat gccgccggcc
      721 actggcggta cgtcatcccg ctggcgcggg cggccgtcgc cgtgggggcc gatgggatca
      781 tcatcgaggt gcacccggag ccggaaaagg ccgtctccga cggcgcccag tccctgaaac
      841 cggagaagtt ctaccggatg atggaagagc tgcgcgtcat cgccggggtg atggggaagg
      901 agatgggggt gccggcatga agaagatccg cgtcaatctc gaccggaaat cggcggatgc
      961 ccacgagatc ttcatcggcc ggggcatcat cgaccgcatg gggctcctca tggccaagaa
     1021 caactgggcg tcgcgctatg tcctcgtgac cgacagccgc gtggccgagc tgcacggcga
     1081 cagggtcctg gcgaccctcc ggggcatgaa cctcgcggtg gacatgatcc agttcccggc
     1141 gggggaggcg tccaagacgg ttcggacctg cctcgatctc gtcgagcggc tgctggccct
     1201 cggggcggac cggaagtccg cgctgatcgc cctgggcggc ggggtggtcg gcgatctgac
     1261 ggggcttgcg gcctccctgt tcatgcgggg cattccctac ctccaggcgc cgacgacgct
     1321 gctcgcgcag gtggacagcg ccatcggggg aaagaccggg gtcaatctcc cggcggcgaa
     1381 gaacatgctg ggcaccttct accagcccag gggcgtcttc atcgacctcg ccttcctgga
     1441 cacgctgccg gagcgggagt tcgccaacgg cctggcggag atcgtcaagt acggcgtcat
     1501 cgaggatccg gagcttctga agcacctggc cgacgaggcc gaggcgatcc gggggcgcga
     1561 cggggcggtc ctggagcgcc tggtcgcccg gtcctgccgg atcaagaagg gcatcgtgga
     1621 gctcgacgag acggagaagg ggctccgccg tatcctgaac ttcggccaca cgatggggca
     1681 cgccgtggaa gccgcgacgg actacggaat ggcgcacggg gaggccgtgg cggtaggcat
     1741 ggtggccgcc gcgaggcttt cggtcgccct cgaccatctg tcctccgacg agggcgaacg
     1801 catcgaggcc gttctgcggg ccatcggcct tcccgtccgg attcccggta acgtgagcgc
     1861 cgagcgtctg ctggacggat tgcggatgga caagaaaaag gaaggcgtgg acgttcactt
     1921 cgtcctgttg aagaagatcg ggattccgtt cgtgaacggc ggggtcccgg aggcgctcct
     1981 gcgcaaaacg atcgaggatt tgcagtcatg aagaagaccc ccctggacga tttgcgggcg
     2041 gccgtgaagg aacgggaccg ggaactggtc cgtctgctga acgagcgggc gaaactgtcc
     2101 gtcgaaatcg gccgggtcaa ggccgttcag aaccggcccg tttacgatcc ggcccaggag
     2161 gcggaggttt acgcacggct ggaggccctg aacgaaggcc ccctgaccgg cccggccctg
     2221 cgggacgtgt tccgggagat tctctcccaa tcccgggccc tgcagacccc catgaccgtg
     2281 gcctatctgg ggccggaggc gtctttttcc cacttggcgg ccctttccca tttcgggcgg
     2341 caggcgcggt acgtccacgc ggcgaccatc gccgaggtgt tcgacgccgc ggagaaggac
     2401 aagggcgtct ggggcatcgt cccggtggaa aattccaccg agggtgccgt aaagcggacg
     2461 ctcgaccgcc tgatgacgac ccctctgacc atccgggcgg agcgctttct caggatcacg
     2521 ctctgcctga tggcgcaacg gtccggacgg aagggaatcc gcaggattta ttcccacccg
     2581 caggcgctcg cgcagtgccg ggggtggctc gaccggaacg tcccccgcgc cgaacgcctg
     2641 gaggtcgcca gcacggcggc ggcggtccgg aaggcgcagg aggagaagga cgccgccgcc
     2701 gtcggcagtc ccatggccgc cgaggcctac ggcctgaagg tggtcgcggc gggcatcgag
     2761 gacagcccgt ggaacaccac gcggtttttc gtgatcggcc ggggcgaggg cgagcccacg
     2821 gggcgggaca agacctctat cctcttcggg acccctcacg ttcccggaac gctcctgaat
     2881 gccctggagc cgctcgcaag ggagggcgtc aacatgacgc ggatcgaatc gtaccccctc
     2941 cgcgaccgga tgtgggaata cctcttcttc gtcgatttcc tcggccatcg ccgggacgag
     3001 aaggtccgcc gctgcctcgc agaaatggcg gccaagacga ccctgatcaa gatcctcggg
     3061 tcctatcccc agggggagga ggcatgaatc ggtgcgctcc cgttcacacc ggcgtccgcc
     3121 cccgcccggt ttcgggggcg gggacctccg gatcaggcat gaggatttgc gttcccgtta
     3181 cggccgccac gaacgaagcc gcgctcgcgc tcatggacag ggtgctggcc gaggccgatc
     3241 tcgtggagat ccgcctggat gccatccgca aaccggacct cgcacgtctg ctgcggggga
     3301 ggcggggtcg ggttctcgtc acgaaccggt ccgcggcgga ggggggcgcc tttgacggct
     3361 ccgaggtccg gcgggtcgcg ctcctgcggg aggcggtcgc cctgggggcc gcctacgtgg
     3421 atgtcgaggc ggcgaccgac ggggggctga tccggtcgct ccgcgaaacc ctgcgggacc
     3481 aggatggcgc cacgaagctc gtcgtctcct ggcacgacgt gacggggacg ccttcggcgc
     3541 gggcgctcag gaagaagctg cgggagctga tggcgtttaa ggccgatgtc gtgaagctgg
     3601 tgacgcgggc ggagaccgtc gcggacgcgt tccgggtgct cgaactcata cccgcggccg
     3661 ggcggcgggg ccaggaaatc gttgccttcg ccatggggcc caggggccgt gccagccgcc
     3721 tgctgtccgt actgatgggc gccagctgga cgttcgccgc gctcgcggcg ggggcggaat
     3781 cggcgccggg gcagatgacg gtcggagaaa cccggcaatc gatgtccgtc ttgacccggg
     3841 gctctcaaaa cggacgcccg tgaaacggag cccgatttcc cgcttctgac ggggagggat
     3901 cgcatgtcga ttcccctgca gacggcgggg agaaatgaag gagtgtgcat cgcgtcatgg
     3961 atgtctacgg cgttatcggc aatcccgtcg gccacagcct ctcgcccctc atgcaccagg
     4021 cggccctgga cgccctcggg atcccggcgc ggtatctcgc ctttgccgtg cgggatctgc
     4081 ccgccgcggt gcaggggatc ctggggctga acctccgtgg tgtcagcgtc accctgccgt
     4141 tcaaaacgga ggtcatgacc ctgctcgacg aggtcgaggc cgatgccagc gccatcggcg
     4201 ccgtcaacac gatcgtcaac gaccacgggc ggctgaccgg cttcaacacc gacggaaccg
     4261 ggctgatccg ggatcttcgg gaggtcatgg atatcgaggg acggcgtttc gccgtcctgg
     4321 gggccggcgg cgccgcacgc gccgccgtgt tcggcctcct gcaggccggg gggattccgg
     4381 tcgtgctcaa ccggaccgcg cagaaagcag aggcgctggc ggcggccttc ggctgcgatt
     4441 tccgtccgct ggcggaagcg gccggcgtgg aggcggactg cctgatcaac acgacgccgg
     4501 tggggatggc gcccggggtc gaacgctcgc cgctgacggc ggacgttctc ggaaggttcc
     4561 gctgggtggt ggacatcatc tacacccccc tgcggacccg tctcctgagg gaggcggccg
     4621 ccgtgggctg ccggacccgc tccggcctcg gcatgttcat ccaccagggg gcggagcagc
     4681 tccggatctg gacgggccgg accccgcccg tggacctgat gcgccgggtg gtggaggcgc
     4741 ggctgatcga agccggctga aggggaaggg aaagaccgca cgcgatggaa gacctgaccg
     4801 aaatcagacc gctccgcgac ctggacgccc gggtgaccct gcccggctcc aagagctaca
     4861 cgcagcgggc gctcgtcatc gccggtttgg cggaggggac gtccgtcctg cgggaccccc
     4921 tgctttcgga ggatacgggg gtcatgatcg acgccctccg cgccctgggc gccgggatcg
     4981 cccgggagga cggggacctc gtcgtcgcgg gaacggccgg gaagatcgcc cggccggaag
     5041 gggagatccg gctcggcaac aacgggacgg ccctgcgctt cctgacggcg gtggtttccc
     5101 tcgggcgggg cgttttcacc ctgaccggcg atccccgcct gtgcgagcgc ccgatcgggc
     5161 ccctcctgga ggccctcgat gccctcggcg tcgacgcccg cagccgcggg gacaacgggc
     5221 accctcccgt cgtcgtcgtc gcggacggcc tgaagggtgg gcaagtggtt ttccgggaca
     5281 tcgagagcag ccagtacgtt tccgcactcc tgctctcggc cccctatgcg tcgggggaca
     5341 cgcgcctcga cctcgcgggt tgcatcccgt cgctcccgta cgtggcgatg acgatcgagg
     5401 cgatggcgac cttcggtcag gacatcgccc gcccggccgc ccacgcgttt gccgtccgcg
     5461 ggaacggcgg gtaccggggg cgggagtacc gcgtcgaggg agatgcctcc agcgcgtcct
     5521 atttcttcct ggccgcggcc ctcacggggg ggcgggtccg ggtggacaac gtccgccccc
     5581 ggaccttgca gggggacatc gggctgctcg ccattctcga agaactcggt tgccacgttc
     5641 ggaggggcga ggactgggtc gaggtggaag gcggccgcct ggctccgggc gacgtcgccc
     5701 tgggcctcgg cgacatgccg gacatggttc cgaccgtcgc cgtcctggcg gccctgcgtc
     5761 ccggccggac gatcatccgg aatgcggccc atctgcggct gaaggagagt gaccgtctcg
     5821 cggccctggc gtcggaactg gtccgggtcg gcatcggggc ccgcgagacg gaagacggtc
     5881 tcgtcatcga cggggggacg ccgcacggcg ccgtcatcga gacgtacaac gaccatcgga
     5941 tcgccatgag ctttgccatc ctggggctcg tggttcccgg cgtccggatc gtcaacggcg
     6001 catgtgtggg gaaatccttt cccggattct ggcaggcgct ggaggggctt tacgcatgaa
     6061 gaacgtagtg ctcgcgggat atcgctgcac gggaaaaacc agcgtcggaa ggggactggc
     6121 ggcccgcctg caccgtccct tttacgacac ggacgatctg gtccgggagc ggacggggat
     6181 gtcggtccgg cg
//
LOCUS       DAOOLJ010000082         6122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_15321_length_6122_cov_17.517884, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000082 DAOOLJ010000000
VERSION     DAOOLJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15321_length_6122_cov_17.517884"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLW16_09425"
     CDS             complement(<1..601)
                     /locus_tag="PLW16_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPB71170.1"
                     /translation="MAGPLTGLKVLDFTTLLPGPYATMTLADLGAEVLAVISGSRPDL
                     AAILPPYLPGTTQSVAGAFLGRGKRSLTLNLKDPRAADIVDRLLGTHDILVEQFRPGV
                     MAKLGFGYEALRKKHPSLIYCSITGYGQTGPFKDRAGHDINYLARSGLMGYQGRRDTG
                     PTLMSMQIADVASGSNHSIIAILAAVIHRQRTGEGQHLDV"
     gene            complement(669..1811)
                     /locus_tag="PLW16_09430"
     CDS             complement(669..1811)
                     /locus_tag="PLW16_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB71171.1"
                     /translation="MDIIKYTDEHKMFRDTVRKFVEKELVPHIEEWEEAGIVPKSVWK
                     KMGDQGFLGTCVPEEYGGPGADFLYSVIILEEMSRANFSGLTASLHSDIIVPYIISFG
                     SEEHKQKYLPGCVTGDIITAVAMTEPNAGSDLAAMKTTAVEDGDSVILNGQKTFISNG
                     INCDLCVVAARDPSVDNPHKAVDLYLVEAGTPGFEKGKQIKKIGWHSQDTSELYFTDC
                     RIPKSNRLGEKGTGFIKLMQKLQQERLVCAIGAQTAAEFMLEVTIKYCQERTVFGKPI
                     SKFQHSQFEIVEMATEIKLGRTFIDKLIMDHMEGQHIVVETSMAKYWITDMAYRVADR
                     CLQLHGGYGYCDEYPISRAWRDIRVTRIFAGTNEIMKVIVARFMGL"
     gene            complement(1956..3170)
                     /locus_tag="PLW16_09435"
     CDS             complement(1956..3170)
                     /locus_tag="PLW16_09435"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HPB71172.1"
                     /translation="MASGIRDKVAIIGMGCSKFGERWDVGAEGLIVEAFQEAIADAGI
                     EKKDIQAAWFGVCFEEINVGKGSVTLGVTLRLPHLPVSRVENFCATGTEAFRAACYAV
                     ASGAYDICLAQGVEKLKDTGYGGLPNWGASAGTLTWQVFANATAPGLFATLASGYGAK
                     YKVKNDDIKKAIAHVSWKSHQNGALNPKAHLRKAVSMDAILAAPIIAWPLGLFDCCGV
                     SDGSACAIVTTPEIAKKLGKKEKDIITIKASQLSVSNGYEGAFNEWQGDHFVTADKCS
                     TACYKEAGITNPKEEISMIEVHDCFSITELVTYEDLHLSDRGQAWKDVLDGMYDRDGK
                     IPSQIDGGLKCFGHPIGASGLRMLYEMYLQLQGKAGDRQLKDPKLGLTHNLGGFPHHN
                     VCAITIIGKYKK"
     gene            complement(3186..5903)
                     /locus_tag="PLW16_09440"
     CDS             complement(3186..5903)
                     /locus_tag="PLW16_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB71173.1"
                     /translation="MIGITSYGAYIPRLRLDRMAIFQAMGWFAPAIISVAQGERSMCN
                     HDEDSLTMAVAAAQDCLIGLDKKKIDAGYLCSTTLPFADRLNAGILATALNLRSDINT
                     ADFTASQRAGTTGLLTALDVVKSGSRKNILVTASDQRMTRPANFYEMWFGDGAAALAV
                     GDKDVVAEFLGSYSLTYDFADHFRGSWCKFDYTWEERWLREEGYSKFIPEAVNGLFKK
                     LKITMDDVQKFVFPCIFKAEHRSIAKKLGATGDKLVDTMHEVCGETGVAHPLLMLVSA
                     LETAKPGDGILLAGFGQGCDALYFKVTENIAKLAERLGVKGTLAKKKSTDNYMKFLKF
                     RELIDPEMGIRNEGPIQTAMTALWRNRKLVLGLVGGKCTVCGTAQLPAADICVNPSCG
                     HVGPMDDYEFAGIPAKVMTFTGDLLAVSVDPPAIYGTVDFAGGGRMLADFTDCERDEI
                     KVGMPVTMSFRKRVKDVNRGFTQYFWKAVPQTDWIPEIRFDGRVAIVTGAGAGLGRAY
                     ALELGKRGAKVVVNDFGGARDGSGAGAAGPADKVVDEIKALGGEAVANYDNVATPEGG
                     AGIVKTAVDAFGKVDILINNAGILRDKGLLKMEPENWAAVLAVHLNGAYNVTRPAFQV
                     MRDQGFGRIVMTTSAAGLYGNFGQTNYSSAKLGLVGFMNTLRLEGAKYNIKVNTIAPV
                     AASRLTEDVMPPEMFAKLSPEYIAGIVLYLCSEECQDTGAIFNAAAGYFSRAAMMTGP
                     GIHLGADKVPTPEDIRDNWEKINSMAGAKHYTELNAAIMDFLMPPAAAGAEAPKGGGG
                     DLDVKGIFEKMPSAFNAAAAAGKDVTFQFNISGPTGGDWAVVIKDGACQVTAGTVDKP
                     TTTIGMADTDFIDLIAGRLDGMKAFSAGKLKVGGDMMKSQLIGKLFKFN"
BASE COUNT         1119 a         1939 c         1782 g         1282 t
ORIGIN      
        1 acacgtcgag gtgctgcccc tcgcccgtcc gctgccggtg gatgacggcg gccagaatgg
       61 cgatgatgga atggttcgat ccggacgcca cgtccgcaat ctgcatcgac atcagggtcg
      121 gtcccgtatc ccgccggccc tgatagccca tgaggcccga acgggccagg tagttgatgt
      181 cgtgaccggc ccggtccttg aagggccccg tctgcccgta gcccgtgatc gagcagtaga
      241 tgagcgacgg gtgcttcttt cggagcgcct cgtagccgaa tccgagcttc gccatgacac
      301 cgggccggaa ctgttcgacg aggatgtcgt gggtgcctag caggcggtcg acgatatccg
      361 ccgcccgggg atccttgagg ttcagggtga gggatcgctt cccccgtccc aggaacgccc
      421 ccgcgacgga ctgggtcgtt ccggggaggt agggcggcag gatcgccgcg aggtccggac
      481 ggctgcccga gatgaccgca agaacctccg cgccaaggtc ggccagggtc atcgtggcgt
      541 aaggaccagg caggagcgtc gtgaagtcca ggaccttcaa tcccgtcaac gggccggcca
      601 tgtctcctcc ttcgtgaacg cggtgcgtga gagggggggg ggcaccgccc cccctcggtg
      661 aacggtgcct aaagccccat gaatcgggcc acgatgacct tcatgatctc attcgtcccg
      721 gcaaagatcc gggtcacccg gatgtcccgc caggcgcgcg agatcgggta ctcgtcgcag
      781 taaccgtaac cgccgtggag ctgcaggcag cggtcggcca cccggtaggc catgtccgta
      841 atccagtact tggccatgga cgtctcaacg acaatgtgct gaccctccat gtgatccatg
      901 atcagtttgt cgatgaaggt ccggccaagt ttgatctccg tcgccatttc cacgatttcg
      961 aactgcgaat gctgaaattt ggagatgggt ttgccgaata ccgtccgctc ctgacagtat
     1021 ttgattgtga cctccagcat gaattccgcg gcagtctgtg caccgatggc acagacgagg
     1081 cgttcctgct ggagtttctg catcagtttg atgaatcccg ttcccttctc gcccaaccgg
     1141 ttgctcttgg gaatccggca gtccgtgaag tacagttccg aagtgtcctg gctgtgccag
     1201 ccgatcttct tgatttgctt gcctttttca aatcccggcg ttccggcctc gaccagataa
     1261 agatccacgg ccttatgggg gttgtccacg gacggatccc tcgccgccac aacacagaga
     1321 tcgcaattga tcccattact gatgaaggtc ttctggccgt tcaggatgac cgaatcgccg
     1381 tcctcgacgg ccgtcgtctt catcgccgcg agatcgctgc ccgcgttggg ctccgtcatg
     1441 gcgaccgccg taatgatgtc gccggtgacg cagccgggga gatatttctg cttgtgctcc
     1501 tcggaaccga aggaaatgat gtagggaacg atgatgtcac tgtggagcga cgcggtaagt
     1561 cccgagaaat tcgcacggga catctcttcg agaatgatca ccgaatagag gaaatccgca
     1621 cctggcccgc catattcttc gggaacgcag gttcccagaa acccctgatc gcccatcttt
     1681 ttccagacgc tcttggggac gatccccgcc tcttcccatt cctcgatgtg gggcaccaat
     1741 tccttttcca cgaacttccg cacggtatcg cggaacatct tgtgttcgtc ggtatatttg
     1801 ataatatcca tggtgtgtct ccttcacggc atgcggataa acacaaattg acggccggaa
     1861 acggcaccgg cagggaaacg cccgtccccc cgcggcgccc gccggggcgg aagggagttt
     1921 ccctacgccc cggccaggtt gaccgcgttg gaaatttatt tcttgtactt accgatgatg
     1981 gtaatggcgc acacattatg gtgggggaac ccgcccaggt tgtgcgtcag gccaagcttg
     2041 ggatccttca actgacgatc tccggccttc ccctgaagct gcaaatacat ctcgtaaagc
     2101 atccgcaagc ccgaggcccc gatcgggtgg ccgaagcact tcaatccgcc gtcaatctgg
     2161 gacggtatct tgccgtcccg gtcatacatc ccgtccaaaa cgtccttcca ggcctggccc
     2221 cgatcagaga ggtggaggtc ttcgtacgtg acgagctccg tgatcgagaa gcagtcatga
     2281 acctcgatca tgctgatctc ttccttcgga ttcgtgatgc cggcttcttt gtagcaagcc
     2341 gtgctgcact tatccgccgt gacgaagtga tcgccttgcc actcgttgaa cgcgccttca
     2401 tacccgttgc tgacggaaag ctgggaagcc tttatggtga tgatgtcttt ttccttcttg
     2461 cccagtttct tggcgatctc cggggtcgtg acaatggcgc atgcggaccc gtcggaaaca
     2521 ccgcagcagt cgaacagacc gagcggccag gcaatgatcg gcgccgccag gatcgcatcc
     2581 atcgacaccg cttttcttaa atgggccttc gggttcagcg ccccgttctg atggcttttc
     2641 cacgaaacat gggcgatggc ctttttgatg tcgtcgttct tgaccttgta tttggcgcca
     2701 taacccgagg ccagcgtggc aaaaagcccc ggagcggtcg cattggcaaa cacctgccag
     2761 gttaacgttc ccgccgaagc tccccaattg gggagtccgc cataccccgt gtccttcagt
     2821 ttctccacgc cttgggccag gcagatgtca taggcgcccg aggcgacggc gtagcacgcc
     2881 gcccggaagg cctccgtgcc cgtggcgcag aaattctcta cccgggagac gggaagatgg
     2941 ggaagacgaa gggtcacccc gagggtcacc gaccccttgc ccacgttgat ctcttcaaaa
     3001 cacacgccga accaggcggc ctgaatgtcc tttttctcga tgcccgcgtc ggcgatcgct
     3061 tcctgaaagg cctcgacgat gagcccctcg gctccgacgt cccatcgctc gccaaacttg
     3121 ctgcaaccca taccgatgat tgcgacttta tctctgattc cagaagccat ggtcatacct
     3181 ccccattaat tgaatttgaa cagcttgccg atgagctggg atttcatcat gtcgccaccg
     3241 accttcaatt tgccggcgct gaaggccttc atgccgtcga ggcgaccggc gatcaggtcg
     3301 atgaagtccg tatcggccat gccgatggtc gtcgtcggtt tatccacggt ccccgcggtc
     3361 acttggcagg cgccgtcctt gatcacaacc gcccaatccc ccccggtcgg accggagatg
     3421 ttgaattgga aggtcacatc cttcccggcc gccgccgccg cgttgaaggc ggagggcatt
     3481 ttttcgaaaa tccccttcac atcgagatcc ccgccaccgc ccttcggcgc ttccgcgccg
     3541 gccgccgccg ggggcatcag gaagtccatg atcgcggcgt tcaactcggt gtagtgcttg
     3601 gcgccggcca tgctgttgat cttttcccaa ttgtcgcgga tgtcttccgg ggtcggcacc
     3661 ttgtccgcgc ccaggtggat cccgggaccc gtcatcatcg cggcccggct gaagtaaccc
     3721 gcggcggcgt tgaagatcgc acccgtgtcc tggcactcct cggagcagag gtacaggacg
     3781 atcccggcga tgtactcggg actcagcttc gcgaacatct ccggcggcat gacgtcttcc
     3841 gtgagccggg aggcggccac cggggcgatg gtgttcacct tgatgttgta tttcgcgccc
     3901 tcgagtctga gcgtgttcat gaagcccacg agccccagct tcgccgagct gtaattcgtc
     3961 tgcccgaagt tgccgtacag gcccgccgcc gaggtcgtca tgacgatccg accgaatccc
     4021 tggtcccgca tcacctggaa ggcgggacgc gtcacgttgt acgcgccgtt cagatgcacg
     4081 gcaaggaccg cggcccagtt ctccggttcc atcttcagaa gacccttgtc gcggaggatg
     4141 cccgcgttgt tgatgaggat gtccaccttg ccgaaggcgt ccacggcggt cttcacgatg
     4201 cccgcgccgc cctcgggtgt cgcgacgttg tcatagttcg ccacggcctc gccgcccaaa
     4261 gccttgatct catcgacgac cttgtccgct ggaccggccg cgccggcccc ggagccgtcc
     4321 cgggcgccgc cgaaatcgtt gacgacgacc ttcgccccgc gcttgcccaa ctccagggcg
     4381 tacgcccggc ccaggcccgc gcccgcaccc gtcacgatcg ctacgcggcc gtcgaagcgg
     4441 atctcgggaa tccagtcggt ctggggaacg gccttccaga aatactgggt aaagccgcga
     4501 ttcacgtcct tcacccgttt ccggaaggac atggtgaccg gcatgccgac cttgatttca
     4561 tcgcgttcgc aatccgtaaa gtcggccagc attcgaccgc cgcccgcgaa atccaccgtt
     4621 ccgtaaatgg ccggaggatc gacggaaacg gccagcaggt cgcccgtaaa ggtcatgacc
     4681 ttcgcgggaa tgccggcaaa ctcgtaatcg tccatcggac ccacatgacc gcacgatggg
     4741 ttcacgcaga tatccgccgc cggaagctgg gccgtcccgc agacggtaca cttcccaccg
     4801 accaaaccga ggaccagttt tcgatttcgc cacagggccg tcatggccgt ctggatcggg
     4861 ccctcgttcc ggatgcccat ctcgggatcg atcagttcac ggaacttcag gaacttcatg
     4921 tagttgtccg tgctcttctt ctttgcgagc gtgcccttga cgcccagacg ttcggcgagc
     4981 ttcgcgatgt tttccgtgac cttgaagtag agggcgtcgc agccctggcc gaagccggcc
     5041 aagaggatcc cgtcgccggg cttggccgtc tcgagggcgg agaccagcat caggagggga
     5101 tgggccacgc cggtctcacc gcaaacctcg tgcatcgtgt cgacgagctt gtcgcccgtg
     5161 gcgcccagct tctttgcgat gctgcggtgc tcggccttga agatgcaggg gaagacgaat
     5221 ttctggacgt cgtccatggt gatcttgagc ttcttgaaca gaccattgac ggcctcgggg
     5281 atgaacttgg agtaaccctc ttcccggagc caccgctctt cccacgtgta gtcgaactta
     5341 caccaggacc cacggaaatg gtctgcgaag tcgtaggtga gactgtagga tccgaggaat
     5401 tccgccacca cgtccttgtc gccgacggcc agggcggccg cgccgtcgcc gaaccacatc
     5461 tcgtagaagt tggccggacg cgtcatgcgc tggtccgacg ccgtcacgag gatgttcttc
     5521 ctgctccccg atttgaccac atccagggcg gtcagcaggc cggtggtgcc cgcgcgctgg
     5581 gaagccgtga aatccgccgt gttgatgtcg ctgcgcaggt tcagggccgt ggccaggata
     5641 ccggcgttca gacgatcggc gaagggcagg gtggtggagc acaggtagcc cgcatcgatc
     5701 tttttcttgt ccaaaccgat caaacagtcc tgggccgcgg ccaccgccat cgtcaggctg
     5761 tcttcgtcat ggttgcacat ggaacgttcg ccctgggcca cgctgatgat cgccggggcg
     5821 aaccagccca tggcctggaa aatcgccatc ctgtccaatc tcaggcgggg aatgtaggcg
     5881 ccgtatgaag tgattccgat catgttctta cctcctcatt cattgcgtag tttgcattca
     5941 gatgtttgag aatcgcacct ccgtcaagcg gcccccttca gaacttgaag agtttttcca
     6001 gaagctggga tttcatcagg tcaccctcga tcttcagttt cccggaggtg taggccttca
     6061 tggcgttcat cttcccgttc atcagatcca gatagtcggc cgccgcgatc ttcagcgtac
     6121 ag
//
LOCUS       DAOOLJ010000083         6040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_15617_length_6040_cov_11.984962, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000083 DAOOLJ010000000
VERSION     DAOOLJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15617_length_6040_cov_11.984962"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1916)
                     /locus_tag="PLW16_09445"
     CDS             complement(171..1916)
                     /locus_tag="PLW16_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPB71174.1"
                     /translation="MKKIRKVLVANRGEIALRIIRTLKERNIEAIAVYETPDQDAYYL
                     RFADRAVMIGREAGKNYLDADRIIRAARKSGADAVHPGYGFLAENAEFAEACEKAGLV
                     FIGPPPEVIRTLGHKVLCKEVARRAGVDPLPGTDSLPAGEAAALDALRQFARAEGYPL
                     MLKATAGGGGRGIRKVEREADLAGQLHVARAEARSAFNDDRLYVERYIESPRHVEVQI
                     LADRHGKVLHLGTRDCSIQRRHQKLLEVAPAHLPAPVIEAIQTAAIEVMKTAGYVNAG
                     TVEFLVDPKTLAFWFMEVNTRLQVEHTVTEMLTGVDIVAEQLRIAAGEALTLDQEQIR
                     LPGAAIEVRINAEDPKNYFLPEAGKRVEVYQPPGGPGIRLDGTVYQGYVIPAVYDSLL
                     VKLTVWGVDWDQAVRRLRRALRDFRLAGPKTTIPFYLKICDEPDFQAGRFQTDYLETH
                     SDLFAYDDDPVWRGGETGRAEHLVLDEAEAPAEPLPPGFVEPPRVKPYLLTKTVEDAA
                     GDGAICSPIKGRVMNIRVSPGETVYAGDVLMILETMKMCTNVITEVTGQVAELYVGVD
                     DTIDVDEPLIRIAAS"
     gene            2216..3247
                     /locus_tag="PLW16_09450"
     CDS             2216..3247
                     /locus_tag="PLW16_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPB71175.1"
                     /translation="MSFIDPAALNSAIVTLAFVGGGVVAGLAFEFLILRQLKKLTAKT
                     EWPGDDVILASLKGAFIALFTTLGLFGAVRSNLTSAEQGATAERVLTVVLILLSTWVL
                     AKIVSGLVQMYSRQVGDGFLSTSMMSNLSRFLVILLGGLTVLQYLNISITPILTALGV
                     GGLGIALALQDTLANLFAGLHIIASKQIKPGDYVKLSTGEEGFVADITWRYTNIRSLA
                     NTITIVPNAQMSKAVVTNFNLPEQETSILVPIGVSYDSDLEKVERVTIEVAREVMQSL
                     EGGVPEFEPLVRYHTFGDSGIHFNVLLRGRHINDQYPLKHALIKRLHRRYREEGIEIP
                     FPTRTLHIR"
     gene            complement(3426..3752)
                     /locus_tag="PLW16_09455"
     CDS             complement(3426..3752)
                     /locus_tag="PLW16_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71176.1"
                     /translation="MVLSELTFWSIMKWVLVILVAGFIGQFGKTFATDLLAKVRSVRA
                     ARRRKPSKNIAVVAGPPAAPSLPDAPAQDLPPGGAPPPPAPLPDKKVLKALAKAQKKA
                     LKARQK"
     gene            complement(3851..4354)
                     /gene="mobB"
                     /locus_tag="PLW16_09460"
     CDS             complement(3851..4354)
                     /gene="mobB"
                     /locus_tag="PLW16_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPB71177.1"
                     /translation="MIPIVSVVGTSNSGKTTLIEKLLAELVRRGYRVATIKHNVHGFS
                     IDHPGKDSWRHKAAGATMTILASPSQVAVVEDVAHDQTFAELAERYVRDVDLILTEGH
                     KRNPHPKIEVLRAVQERELLSKPEDGLVAVASDRPLDVGVPCLDIDDAAGLADLIEAR
                     FLGGRGR"
     gene            complement(4496..5887)
                     /locus_tag="PLW16_09465"
     CDS             complement(4496..5887)
                     /locus_tag="PLW16_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPB71178.1"
                     /translation="MERLLRVTLVVIALLMMALPAVAGAGDSAAAPGHAAALTFFWIA
                     VILIAAKLSSLVEKIGQPSVLGELVIGVLMGNAVLVGIPFFEPLKQDAVIAFLSELGV
                     VILLFQIGLESNIEKMKAVGLKAFAVAVVGVVVPFVLGTYLVGPWLLPGLAFHAYLFI
                     GAVLTATSVGITARVFQDLKKLQTPEAQIVLGAAVIDDVLGLIILAVVSAIVTVGSVS
                     IGLIGWLIAKAILFLVGSILVGQLLAAQLGKWLAKVQTGMGMKFTLAICFCLVLAFLA
                     QQIGLAPIVGAFAAGLVLDPVHFGYFKDPKVVSDVKAAMEGCAPDVKQKVEKAIEPHA
                     YSHVEDLIEPVAFLLVPIFFVMTGMSVRLETLFDVPILLLALGITAAAFLGKIVSGLV
                     AGNVNKSIVGFGMIPRGEVGLIFAMMGKSLRVISDSVFAALIIMVILSTLITPPVLTY
                     LLKRQDRKSRPSA"
BASE COUNT         1097 a         2000 c         1848 g         1095 t
ORIGIN      
        1 agacaccgga ccagacactc cccgcacaaa tcgcacttcg acgcatccca catgagccat
       61 ctcctccttt attcgtcgtt gaaaaccgtt gaagatcgat atgatcccgc acaacggggc
      121 cttttcggcc cggtgccgcc cccgcggcag gcggagggat ccgtcgggtc ttatgacgcc
      181 gcgatccgga tcagcggttc gtccacgtct atcgtgtcgt cgaccccgac atagagctcc
      241 gccacctgcc cggtcacctc ggtgattacg ttggtgcaca tcttcatcgt ctcgaggatc
      301 atgagcacgt caccggcgta gacggtctct ccggggctta cgcggatgtt catgacgcgg
      361 cccttgatgg gggaacagat agccccgtcc ccggccgcat cctccaccgt cttggtcaga
      421 agatagggtt tgacgcgggg cggctcgacg aagcccgggg gcagcggttc ggcgggcgcc
      481 tcggcttcat cgagcaccag atgctcggcc cggcccgttt cgccccccct ccagaccggg
      541 tcgtcgtcat aggcgaaaag atcggaatgg gtctccagat aatccgtctg gaagcggccg
      601 gcctggaaat cgggttcgtc acagatcttg agatagaacg ggatggtggt cttcgggccc
      661 gccaggcgga aatcgcggag cgcccggcgg agcctccgca cggcctggtc ccagtccacc
      721 ccccagacgg tcagtttgac caggagggaa tcgtacacgg cgggaatcac atatccctgg
      781 tacaccgtcc cgtcgaggcg gatgcccggc ccgcccggag gctggtagac ctcgacgcgc
      841 tttcccgcct cggggagaaa atagtttttc ggatcctcgg cgttgatgcg cacctcgatc
      901 gccgccccgg gcagacggat ctgttcctgg tcgagcgtca gggcctcccc cgcggcgatc
      961 cggagctgtt ccgccacgat gtccacgccg gtcagcatct ccgtcaccgt gtgttccacc
     1021 tgaaggcggg tgttgacctc catgaaccag aaggcgagcg tctttgggtc gacgaggaac
     1081 tccaccgtcc cggcattgac gtatccggcg gttttcatga cctcgatcgc cgccgtctgg
     1141 atcgcctcga tcaccggcgc cggcagatga gcgggggcga cttccaggag tttctggtgc
     1201 cgccgctgga ttgaacagtc ccgcgtcccc agatggagga cctttccgtg acggtcggcc
     1261 aggatctgga cctccacatg gcgcggggat tcgatatagc gctccacgta gaggcggtcg
     1321 tcgttgaagg ccgagcgggc ctcggcccgg gccacgtgaa gctggccggc caggtccgct
     1381 tcccgctcca ccttccggat gccccggccg ccgccgccgg cggtggcctt gagcatcagc
     1441 ggatagccct cggcccgcgc gaactgccgg agggcatcga gcgccgcggc ctccccggcg
     1501 ggcaggctgt ccgttcccgg cagggggtcg acgccggccc ggcgggcgac ctccttgcag
     1561 aggaccttgt ggccgagcgt ccggattacc tccggcggcg ggccgatgaa gacgaggccg
     1621 gccttttcgc aggcctcggc gaactcggcg ttctcggcga gaaacccgta gccgggatga
     1681 acggcgtcgg cgccgctttt ccgggccgcc cggatgatcc ggtcggcatc cagataattt
     1741 ttccccgcct cccggccgat catcacggcc cggtcggcga accgcaggta gtaggcgtcc
     1801 tggtcgggcg tctcgtagac ggcgatggcc tcgatattgc gctctttcaa ggtccgaatg
     1861 atccggaggg cgatctctcc ccggttggcg accagcacct ttctgatctt tttcattttc
     1921 ccttcaaaat cctcccccgc gggctttggc tccggggccg cgccgggcgg gggcgggaga
     1981 gattatcgaa agcagtgtgt tttcttatca cgtcttcccc ggggtgtgaa ccgaaatttc
     2041 acgtgggcgg caccccggcc gggaacgccg tgcgccaccg gaaacacccg aaagcagttg
     2101 gcagggccgc cggcctgtgg tatcgtgccg ttgtcccggt tcacgcgacg gacggcagga
     2161 aacgatcgcc gtccttcgtc ccggccgggg agccatcccg tccgtgaggt gaatcatgtc
     2221 ctttatcgat cctgccgcgc tgaactcggc aatcgtcacg ctggccttcg tcggtggcgg
     2281 cgtcgtcgcc ggcctggcct tcgaattcct gatcctgagg caactgaaga aactgactgc
     2341 gaaaacagag tggccgggcg acgatgtcat cctggcatcc ctgaagggcg ccttcatcgc
     2401 cctgttcacg accctcggcc tgttcggcgc cgtacggtcc aatctgacgt ccgccgagca
     2461 gggcgctacc gccgagcggg tcctgactgt cgtcctgatc ctcctgtcga cctgggtgct
     2521 cgcgaaaatc gtttcggggc tcgtccagat gtacagccgg caggtcggcg atggcttcct
     2581 gtcgacctcg atgatgtcca acctgtccag gttcctcgtg attcttctcg gcggcctgac
     2641 ggtgctccag tacctcaaca tctccatcac gccgatcctg acggccctgg gcgtgggtgg
     2701 cctgggcatt gccctggcgc tccaggacac gctcgcgaat ctcttcgccg gcctgcacat
     2761 catcgcgtcg aagcagatca agccgggaga ttatgtcaag ctctccaccg gcgaggaggg
     2821 gttcgtcgcg gatatcacgt ggcgttacac caatatccgg tcgctcgcca acacgatcac
     2881 catcgtcccc aacgcccaga tgtccaaggc ggtggtcacc aacttcaacc tccctgagca
     2941 ggagacgtcg atcttggttc cgatcggcgt cagctacgac agcgatctgg aaaaggtcga
     3001 acgcgtgacg atcgaggtcg cccgggaggt gatgcagtct ctggaggggg gcgtccccga
     3061 gttcgaaccg ctcgtccgtt accacacctt cggggattcc ggcatccatt tcaacgttct
     3121 cctgcgcggg cggcatatca acgaccagta tcctctcaag cacgcgctca tcaagcgcct
     3181 gcatcggcga taccgggagg aaggaatcga aatccccttc ccgacccgga cgctgcacat
     3241 ccgttgaagt actgcggacg ccttccgcct gacgcggagg ttacccgtgg ggtcccctgt
     3301 gtcgggctcc ggcgtttcgt ggagcaggcc cgcaaggatt acaacatcgc tggattcggt
     3361 gacatgacgc ccggaacgat cgcccgaccg accgcgggtt tttcgcgcga agccgggcga
     3421 cggcgctatt tctgccgggc tttaagggcc ttcttctgcg ccttggcgag cgccttcagg
     3481 acctttttgt ccggcagagg agcggggggc gggggggcgc cgcccggtgg gagatcctgg
     3541 gccggcgcat ccggcaggga aggggccgca ggggggccag caacgaccgc aatgtttttc
     3601 gagggtttcc gccgcctggc cgcccggacc gaccggactt ttgccagcag gtcggtggcg
     3661 aaggtcttcc cgaattggcc gatgaagccg gccacgagga tcacgagcac ccacttcatg
     3721 atggaccaga aggtcagctc cgacagaacc acgggatcct cccttgcgtt gggtcggttg
     3781 cagtcccgcc cctagagcac ccgggacgcg ttcgcccggg cacacgagag cggcgtcggg
     3841 cggaggccgt ttatcgcccg cggccgccga ggaaccgcgc ctcgatgaga tcggccagcc
     3901 cggccgcatc gtcgatgtcc aggcagggga cgccgacatc cagcggccgg tcgctcgcca
     3961 cggcgaccag gccgtcctcc ggtttggaca ggagttcccg ctcctgaacg gcccgcaaca
     4021 cctcgatctt ggggtgcgga ttgcgtttgt ggccctccgt caggatcagg tccacgtccc
     4081 ggacatagcg ttccgccagt tccgcaaacg tttgatcgtg cgccacatcc tccacgaccg
     4141 ccacctgcga cggcgacgcg aggatcgtca tcgtcgcccc ggccgccttg tgccgccaac
     4201 tgtccttgcc gggatggtcg atcgagaagc cgtggacgtt gtgcttgatc gtcgccaccc
     4261 ggtatccccg gcggaccagt tccgccagga gtttttcgat cagggtcgtc tttccggaat
     4321 tcgaggttcc gaccaccgaa acaatgggga tcatcgcttt tgtcctcatc cgcctgcccg
     4381 aggcgcccct gccccgcaag agcctcctgt cggcagcggc cggggccggc cggcgacgca
     4441 tgcggcgcac caatgccgct cggaggcgac cccgcccgtg atcggacgcc ccccctcaag
     4501 ccgagggtcg agatttccgg tcctgccgtt tcagaagata ggtcaagacc ggcggggtga
     4561 tcagcgtgct cagaatcacc atgatgatca aggcggcgaa cactgaatcc gagatcaccc
     4621 tcagggattt ccccatcatg gcaaaaatca aaccgacctc gccgcgcggg atcatgccga
     4681 acccgacgat ggatttattc acgtttccgg cgacgagacc cgaaacgatc tttcccagaa
     4741 acgcggccgc tgtaatcccc agggccagga gaaggatcgg cacgtcgaac agcgtctcca
     4801 gtctgacgct cattcccgtc atgacaaaaa aaatggggac cagcaagaac gccaccggtt
     4861 caatcaaatc ctccacatgg ctgtacgcat ggggttcgat ggccttctcg accttttgtt
     4921 tgacgtccgg cgcacagcct tccatggccg cctttacatc gcttacgacc ttgggatcct
     4981 tgaagtatcc gaaatgcacc gggtccagaa ccaggccggc ggcgaaggcg cccacgatcg
     5041 gggccaagcc gatctgttgc gccagaaacg ccagcaccag gcagaaacaa atggccaggg
     5101 tgaatttcat tcccatgccc gtttgaacct tcgcgagcca tttgcccagt tgcgccgcca
     5161 gcagttggcc gaccaggatc gatccgacca gaaacaggat cgctttggcg atcagccagc
     5221 cgatcagccc gatgctgaca ctgcccaccg tcacgatggc cgacaccacg gccaggatga
     5281 tcaatcccag gacgtcgtcg atcacggcgg cgcccaggac gatctgggcc tccggagtct
     5341 gcagcttttt gaggtcctga aacacccgtg cggtgatccc caccgacgtg gcggtcaaaa
     5401 cggccccgat gaacagatag gcatggaagg ccaaaccggg cagcagccac ggacccacca
     5461 ggtaggttcc caaaacgaac ggcaccacca cgccgacgac ggcgaccgca aaggccttga
     5521 gacccacggc cttcattttt tcgatgttgg attccagccc gatttgaaac aggagaatga
     5581 cgacgcccag ttccgacagg aaggcgatga cggcatcctg cttcagcggc tcaaaaaacg
     5641 ggatgcccac caggaccgca ttgcccatca gcaccccgat caccagctcg cccaaaacgg
     5701 acggctgacc gatcttttcc accagactgg acagtttggc ggcgatcaga atcacggcga
     5761 tccagaagaa cgtcaaggcg gcggcgtgac ctggggcggc ggctgaatcg cccgccccgg
     5821 caacggcggg aagtgccatc atcaggaggg cgatgacgac aagcgtcact ctcagcaatc
     5881 gttccatgac acgtccccga aaatttcccg tcacaaccct ctgtgacggg cgttgtgacg
     5941 ggagtaaacc acagccgggg ccctcctgtc aaaggtttgc tgatcgaaaa taatcccttg
     6001 aacttcctca ggaaaggata tatacagcgg gcgacttgaa
//
LOCUS       DAOOLJ010000084         6037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_15622_length_6037_cov_18.952190, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000084 DAOOLJ010000000
VERSION     DAOOLJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15622_length_6037_cov_18.952190"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="nrdR"
                     /locus_tag="PLW16_09470"
     CDS             <1..447
                     /gene="nrdR"
                     /locus_tag="PLW16_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPB71179.1"
                     /translation="HPENKVIDSRVSKDNNAIRRRRECLSCERRFTTYEFVEEVLPMV
                     VKKDGRREPFDRTKILSGVKKACEKRPISMDDMEALVDRIEQYCQDTQAREIPAALIG
                     ERVMGELEKLDGVAYVRFASVYREFRDVRDFINELKDFLNGKEDVK"
     gene            513..1169
                     /locus_tag="PLW16_09475"
     CDS             513..1169
                     /locus_tag="PLW16_09475"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPB71180.1"
                     /translation="MFTGIIQGTGIIRGLTRQGADARLEVETDLSLNDVALGDSIAID
                     GACLTVTARTARTFTADVSAETLSRTTLGAARVGGRANLEKALRLGDFLGGHLVLGHV
                     DGIGRIAAQTLRANSVLIGVDVDAALMRYVVEKGSVAVDGISLTVNRCEKNRFYVNMI
                     PHTADATTLGRKRAGETVNIETDIIGKYVERLAAPYTGTAPAGGGLSRDLLSRHGFIK
                     "
     gene            1202..2404
                     /locus_tag="PLW16_09480"
     CDS             1202..2404
                     /locus_tag="PLW16_09480"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPB71181.1"
                     /translation="MSVSTIQEAIEDIRNGKMVILVDDEDRENEGDLCMAAEFITPEA
                     INFMARYGRGLICLTLTEEHADRLGLTPMTRDNRSRFGTAFTVSIEAKQGVTTGISAY
                     DRATTIRAAVADGAKAEDLVSPGHIFPIRARKGGVLFRTGQTEGSVDLARLAKLKPTG
                     VICEVMKDDGTMARMPDLELFSKEHGLKICTIADLIDFRMQHESLIRRAATAGVPTRY
                     GGDFTLTVYENDVDDTLHIALVKGEIRPEDEVLVRVHSECVTGDLFGSERCDCGDQLH
                     TAMKMVDQEGKGVIVYMHQEGRGIGLVNKIKAYALQEQGHDTVEANLALGFKADLRDY
                     GIGAQILVDQGVRKMRLMTNNPKKVVGLEGYGIKIVGRVPIEIQPNENNIKYLTTKKK
                     KMGHMLKI"
     gene            2428..2901
                     /gene="ribE"
                     /locus_tag="PLW16_09485"
     CDS             2428..2901
                     /gene="ribE"
                     /locus_tag="PLW16_09485"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPB71182.1"
                     /translation="MPKLMEGKIVAKGMKFAIAASRFNDFICGRLIEGAMDALTRAGA
                     DEKDIQIVKVPGAFELPLTAKKLAKSGRFDAVICLGAVIRGATPHFEYISAEVSKGIA
                     SVGLDTEVPVAFGVLTTDTIEQAIERAGTKAGNKGWDAAMTAIEMVDLFKKLSAL"
     gene            2979..3449
                     /gene="nusB"
                     /locus_tag="PLW16_09490"
     CDS             2979..3449
                     /gene="nusB"
                     /locus_tag="PLW16_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB71183.1"
                     /translation="MRYRRKAREAALQVLYALDVLEWIDVQDALALFWAHYGAPEEVK
                     PFATVLVEGTWLNRRSIDELIRGCSENWSLERMARVDKGILRMAVYEFLHCPDIPPKV
                     TLNEAIDLGKIFGSENSGAFINGILDALYAKLREQNLLADVTLPGPLAGGIDAP"
     gene            3506..>6037
                     /gene="leuS"
                     /locus_tag="PLW16_09495"
     CDS             3506..>6037
                     /gene="leuS"
                     /locus_tag="PLW16_09495"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPB71184.1"
                     /translation="MNAKYAPQEIEEKWQRIWTERETFKVTEDPARRKYYLLEMFPYP
                     SGRIHIGHVRNYTIGDVVARYKRMRGFNVLHPMGWDSFGMPAENAAIDRGIHPSVWTN
                     DNIANMRRQLQRMGFSYDWDREISTCEPDYYRWEQLFFLKMFEKGLAYKKRSSVNWCP
                     QCKTVLANEQVEAGLCWRCGAEVGEKDLEQWFFKITAYVEELLESCNRLPGWPEQVLT
                     MQKNWIGKSYGCELSFPMEDGREDIRVFTTRQDTIYGATFMLIAAEHPLVMELARGKA
                     CEADVQAFVEKVKKQDKMMRTSDYYDKEGVFLETFCLNPVTNFRMPIYAANFVLADYG
                     TGCVMAVPTHDQRDFEFAERYNLEKIVVIQPPGTTLDPKTMTEAYVDEGLLVHSGPFD
                     GMENLKALEAIAEHLEAIGRGKKTVQYRLRDWGISRQRYWGAPIPVIHCEKCGSVPVP
                     EQDLPVVLPRDIDLTGTGESPLARHDAFWKTICPVCSGPARRETDTMDTFVESSWYFD
                     RFCSPACNIKPGLDRRQVDYWMPVDQYIGGIEHAILHLLYARFYTKMLRDFGVVGVDE
                     PFTNLLTQGMVCKETMRCAEHGYLYPDEAKDGRCAHCGREVQVGKTEKMSKSLKNVVD
                     PDYLIQKYGADTVRMFCLFAAPPEKDLEWSDQGVDGSFRFINRTWRIVMDYLDDIRET
                     SPFAGDRELTGDLKALRRKTHQVIRKVTSDIEDRFHFNTAISAVMELVNVLYQTKRPE
                     KSDAVGLAVIRETIEAIVVLLSPIVPHIAEELWTLFGKTDALADTAWPTFDAGIAAEE
                     EITIVLQINGKVRSRITVPADESEARIKAAARADKRICEFIGDRPV"
BASE COUNT         1258 a         1907 c         1915 g          957 t
ORIGIN      
        1 catcccgaaa acaaggtcat cgactcccgg gtcagcaagg acaacaacgc catccggcgg
       61 cggcgggaat gcctcagttg cgagcgccgg ttcacgacct acgagttcgt cgaggaagtc
      121 ctgccgatgg tcgtgaagaa agacggccgc cgcgagccgt tcgaccggac caagatcctg
      181 tccggggtga agaaggcctg cgaaaagcga ccgatcagca tggacgatat ggaggcgctg
      241 gtggaccgca tcgagcagta ttgccaggac acccaggccc gggagatccc ggccgccctg
      301 atcggcgagc gcgtcatggg cgaactcgaa aagctggacg gcgtggccta cgtccgcttc
      361 gcctcggttt accgagagtt ccgggacgtc cgggacttca tcaacgagct caaggatttc
      421 ctgaacggga aggaagacgt gaaataggga tgcgggtccg aacccgcatc ggacgcgggg
      481 aatcggccgg accggccggc gggagcgatt caatgtttac gggcatcatc cagggaaccg
      541 gaatcatccg cggtctgacg cgccaggggg ccgacgcccg cctggaggtt gagaccgatc
      601 tgtccctgaa cgacgtggcc ctgggcgaca gcatcgcgat cgacggcgcc tgcctgacgg
      661 tgacggccag gaccgcccgg accttcacgg ccgacgtctc ggcggaaacc ctctcccgga
      721 cgaccctcgg cgccgcgcgg gtcggcggca gggcgaacct cgaaaaggcc ctgcgcctcg
      781 gggattttct cggcgggcac ctcgtcctgg gccatgtgga cgggatcgga cggatcgccg
      841 cccaaaccct ccgggccaac tccgtgctca tcggggtgga cgtggacgcg gcgctgatgc
      901 gctacgtcgt ggagaagggc tcggtggcgg tggacggcat cagcctgacg gtaaatcgct
      961 gtgaaaaaaa ccgcttttat gttaatatga tcccgcatac ggcggacgcg accaccctgg
     1021 gtcgcaaacg ggccggagag accgtcaaca tcgagacgga catcatcggc aaatacgtgg
     1081 aaagactggc cgcgccctat acgggaacgg cgccggccgg cggcgggttg agcagggacc
     1141 tcctctcccg tcatggattc atcaaatagc cgtcccgaaa aacgattcaa gaaggtgttc
     1201 aatgagtgtc agcacaatcc aggaagccat cgaggatatc cgcaacggca agatggtcat
     1261 tctcgtcgat gacgaggatc gcgaaaacga gggcgatctc tgcatggccg cggagttcat
     1321 taccccggag gcgatcaatt tcatggcccg ctacggccgc ggcctgatct gcctcaccct
     1381 gacggaggaa catgccgacc ggctcggcct gacccccatg acgcgggaca accgctcccg
     1441 cttcggcacg gccttcacgg tctccatcga ggcgaagcag ggcgtcacca ccggcatctc
     1501 cgcctacgac cgcgcgacga cgatccgggc ggccgtcgcc gacggcgcca aggcggaaga
     1561 cctcgtcagc ccgggccaca tcttccccat ccgggcccgc aagggcggcg tgctgttccg
     1621 caccggccag acggagggct ccgtggacct ggcccgcctc gccaagctga agcccaccgg
     1681 ggtcatctgc gaggtcatga aggacgacgg gacgatggcc cgcatgcccg atctggagct
     1741 cttttcgaaa gagcacggcc tcaagatctg caccatcgcc gacctgatcg attttcgcat
     1801 gcagcatgaa tccctgatcc ggcgggcggc gacggccggc gtccccaccc gctacggcgg
     1861 cgacttcacg ctgacggtct atgaaaacga cgtggacgac accctccata tcgccctggt
     1921 gaagggggaa atcaggcccg aggacgaggt cctcgtccgg gtgcactcgg agtgcgtcac
     1981 cggggacctg ttcggttcgg agcggtgcga ctgcggggat cagctccaca cggcgatgaa
     2041 gatggtcgat caggagggca agggcgtcat cgtttacatg caccaggaag gccgcgggat
     2101 cggcctggtc aacaagatca aggcctatgc ccttcaggaa caggggcatg atacggtcga
     2161 ggccaatctg gccctcggct tcaaggcgga cctccgggat tacggcatcg gcgcccagat
     2221 cctcgtcgac cagggcgtgc gcaagatgcg cctgatgacg aacaacccca agaaggtcgt
     2281 gggcctcgag gggtacggga tcaagatcgt cggacgggtc ccgatcgaga tccagcccaa
     2341 cgaaaacaac atcaagtacc tcaccacaaa gaagaaaaag atggggcata tgctcaagat
     2401 atgaagggtg aaaaggagat ttccgtcatg ccgaaactca tggaagggaa gattgtcgcc
     2461 aaggggatga agttcgccat cgcggccagc cgcttcaacg acttcatctg cggccggctg
     2521 atcgagggcg cgatggacgc actgacccgc gcgggggcgg acgagaagga catccagatc
     2581 gtcaaggtcc ccggggcctt cgaactgccc ctgacggcga agaagctggc caaatcggga
     2641 cgcttcgacg cggtgatctg cctcggcgcc gtcatccggg gcgccacgcc ccatttcgag
     2701 tacatcagcg ccgaggtctc caagggcatc gccagcgtcg gtctggacac agaggtcccc
     2761 gtcgcgttcg gcgtcctgac gacggacaca atcgagcagg ccatcgaacg ggcgggcacg
     2821 aaggcgggca acaagggctg ggacgccgcc atgacggcca tcgagatggt ggacctgttc
     2881 aaaaagctct cggcactatg atcttttcca ggacgcagcg gcggaaaatc ctggccggcg
     2941 gattccggga agagtccccg cggctgacag gggcttgaat gaggtaccgg agaaaggcac
     3001 gcgaggcggc cttgcaggtt ctttacgcgc tggacgtcct ggagtggatc gacgttcagg
     3061 atgccctagc cctcttctgg gcgcattacg gcgccccgga ggaggtcaag cccttcgcca
     3121 cagtcctcgt cgagggaacg tggctgaacc gtcggtccat cgatgaactg attcggggct
     3181 gctccgaaaa ctggagtctg gaacggatgg cccgggtgga caagggcatc ctccggatgg
     3241 ccgtctacga atttctccat tgccccgaca tccccccgaa agtgaccctc aacgaggcca
     3301 tcgatctcgg caagatcttc ggttcggaga actccggcgc cttcatcaac ggcattctgg
     3361 acgcccttta cgccaagctt cgcgagcaga acctcctggc cgacgttacc ctgccggggc
     3421 cgctggccgg cgggatcgat gcgccctgaa agaaacggcc gcctgcggcg gccggtcgat
     3481 accatcaatg gaggataaca acagcatgaa cgcaaaatac gctcctcagg aaatcgagga
     3541 gaagtggcag cggatctgga ccgagcggga aaccttcaag gtgacggagg acccggcaag
     3601 gcgcaaatac tatctgctcg agatgttccc ctacccctcc ggccgcatcc atatcggaca
     3661 cgtccgcaat tacaccatcg gcgacgtcgt ggcgcgttac aagcggatgc ggggattcaa
     3721 tgtcctgcac ccgatgggct gggattcgtt cggcatgccg gcggagaacg ccgccatcga
     3781 ccgcggcatc catccctctg tctggacgaa cgacaacatc gccaacatgc gccggcagct
     3841 tcagcgcatg ggcttcagct atgactggga ccgcgagatt tcgacgtgcg agccggacta
     3901 ttaccgctgg gaacagctct tcttcctcaa gatgttcgag aagggcctgg cctacaagaa
     3961 gcgctccagc gtcaactggt gcccccagtg caagaccgtc ctcgccaacg aacaggtcga
     4021 agcgggcctg tgctggcggt gcggcgccga agtcggcgag aaggacctgg aacagtggtt
     4081 tttcaagatc accgcctatg tggaagagtt gctcgagtct tgtaaccggc tccccggctg
     4141 gccggagcag gtcctgacga tgcagaagaa ctggatcggc aagagctacg gttgcgaact
     4201 ctcctttccg atggaggacg ggcgcgagga catccgggtg ttcacgacac ggcaggatac
     4261 gatttacggc gcgaccttca tgctcatcgc cgcggaacat cccctggtca tggaactggc
     4321 gcggggaaag gcctgcgagg cggacgtcca ggcgttcgtg gaaaaggtca agaagcagga
     4381 caagatgatg cggacctccg attactacga caaggagggc gttttcctcg aaaccttctg
     4441 cctgaacccc gtgacgaatt tccgcatgcc catctatgcg gctaacttcg tgctcgccga
     4501 ctacggcacg ggatgcgtca tggcggtgcc gacgcacgac cagcgcgact tcgaattcgc
     4561 cgagcgctac aacctcgaaa agatcgtggt catccagcct cccggcacga ccctcgaccc
     4621 gaaaaccatg acggaggcct acgtcgacga agggctcctc gtccactccg gaccgttcga
     4681 cggcatggag aacctgaagg ccctggaggc gatcgcggaa cacctcgaag ccatcggccg
     4741 cggcaaaaag accgttcagt accgcctgcg cgactggggc atctcccgcc agcgctactg
     4801 gggggcgccg atccccgtga tccactgcga aaagtgcggg tccgttcccg tccccgagca
     4861 ggacctgccc gtcgtcctgc cgcgggacat cgacctgacc ggcacggggg aatcccccct
     4921 ggcacgccac gacgccttct ggaagacgat ctgtcccgta tgcagcgggc cggcccgacg
     4981 ggaaacggac acgatggaca ccttcgtcga gtcctcctgg tacttcgacc gcttctgctc
     5041 gcccgcctgc aatatcaagc cgggcctgga ccggaggcag gtggactact ggatgcccgt
     5101 ggaccagtac atcgggggga tcgaacacgc catcctgcac ctcctgtacg cccgcttcta
     5161 caccaagatg ctccgggact tcggcgtggt cggggtggac gagcccttca cgaacctcct
     5221 cacgcagggc atggtctgca aggagacgat gcgctgcgcc gaacacggct acctctatcc
     5281 cgacgaagcc aaggacggcc gctgcgccca ctgcggccgg gaggtccagg tcggcaagac
     5341 ggagaagatg tcgaagtccc tgaagaacgt cgtcgacccg gactacctca ttcaaaaata
     5401 cggggccgac acggttcgga tgttctgcct gttcgccgcc ccgccggaga aggatctgga
     5461 atggagcgac cagggggtgg acggctcgtt ccgtttcatc aaccggacct ggcggatcgt
     5521 catggactac ctggacgaca tccgcgaaac ctcccccttc gcaggcgaca gggagctgac
     5581 gggggacctg aaggcgctcc ggcgcaaaac ccatcaggtc atccgcaagg tgacctccga
     5641 catcgaggat cgctttcact tcaacacggc catcagcgcc gtcatggagc tcgtcaacgt
     5701 cctgtaccag acgaagcgtc cggagaaatc ggacgccgtc ggcctcgccg tgatccgcga
     5761 aaccatcgag gcgatcgtcg ttctgctctc ccccatcgtc cctcacatcg cggaggagct
     5821 gtggacgctg ttcggaaaga cggacgccct cgccgacacc gcctggccca ccttcgacgc
     5881 ggggatcgcc gcggaagagg aaatcaccat cgtcctgcag atcaacggca aggtgcggag
     5941 ccgcatcacg gtccccgcgg acgaatcgga agcgcggatc aaggcggccg cgcgcgcgga
     6001 caagcgaatc tgcgaattca tcggggaccg gccggtc
//
LOCUS       DAOOLJ010000085         6029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_15655_length_6029_cov_20.247573, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000085 DAOOLJ010000000
VERSION     DAOOLJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15655_length_6029_cov_20.247573"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PLW16_09500"
     CDS             <1..1605
                     /locus_tag="PLW16_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB71185.1"
                     /translation="FFTFLVNNELFRLRLKLSRSSHISHEEFADVEARFLEGRFTPEI
                     LEQFKDMLDYFGQAPIIVRSSSLLEDSFGNAFAGKYRSEYCANQGSPEERLEAFLTAV
                     KLVYASTLNPDALTYRRMRGLGESDEQMAILVQRVSGMPFNRYFFPTLAGVAFSRNLY
                     AWTSRIDPAQGMIRLVLGLGTRAVNRVGDDYPRMIAVSHPKLRPETGTGIAKYSQWEM
                     DVIDLEENRLETKSVREVFRDGEYPNLPMLVSLMKDGYLSDPFTGRLPPGTNKFVLTF
                     NNLINRTDFVKIVGEMLSRLEAAYGHPLDTEFTAAVDPEGRVRVNLLQCRPMRLPGTT
                     GPVALPAGLPKERVLFRSGRTISGGVVERIRYILYIDPKRYATISDADVKKTLGRVVG
                     RINEHPGIAGRPLVMMGPGRWGSSNIDLGVNVSYADINNTSVLVEIAREEAGHVPEVS
                     YGTHFFLDLVEARIIYLPVYPNDPEADFQHAFFAETANALTDLLPEAGRYAPYVHLID
                     LPAATGGRCARLVADPESQQAVCYIE"
     gene            1682..2503
                     /gene="dapF"
                     /locus_tag="PLW16_09505"
     CDS             1682..2503
                     /gene="dapF"
                     /locus_tag="PLW16_09505"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPB71186.1"
                     /translation="MIEFTKMSGSGNDFILIDNRARTLQVPDITAFVQAVCARKIAVG
                     ADGLILIEPSDRVDFRWRFFNADGSEVEMCGNGGRCAARFAFLKGIAKERMSFETGAG
                     VIDAEVRGDVVKLRLTDPRDLAINQSLSVDGKSLEVHSINTGVPHVVHFVNDLEHTDV
                     FHLGRMLRHHAQYQPAGTNANFVAAIDPHALEIRTYERGVEDETLACGTGAVASALIS
                     AWKGLVESPVEVRVRSGETLCIHFTKKAGGFEKIYLEGRAKVVYEGRLWEEAYRP"
     gene            2514..3680
                     /locus_tag="PLW16_09510"
     CDS             2514..3680
                     /locus_tag="PLW16_09510"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPB71187.1"
                     /translation="MQTADRLTKIPPYLFMELRKKINQAKADGVDVISLAIGDPVEPT
                     PAPIIAELCRRAQDSENHRYPTDEEKGMLAFRREVARWYGTRYGVDLKPETEILGLIG
                     SKEGCHHFVLARINPGDVVLMTDPGYPAYRASILMGGGEPYNVPILRENGYLPVFADI
                     PSDVAKRARGMFLNYPNNPTGACATKPFLNNLVAFARQFDIAVCYDNPYSEILFGGQE
                     RISFLMAAGAKDVGIELNSLSKPFNMCGWRIGMAAGNPDLIAAISKVKENTDSGIFNP
                     IQYAAIHALAKNAPAVEDMLAVYARRRERVLAALARIGITFDAPKGTFYLWVPVPKGM
                     TSLEFTNRLFDKTAVVVASGTAYGQYGEGFVRISLTVPDDRLTEAMRRIEQEFA"
     gene            3906..4934
                     /locus_tag="PLW16_09515"
     CDS             3906..4934
                     /locus_tag="PLW16_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPB71188.1"
                     /translation="MEIGIVGLPQSGKSTLFEIMTGVRGRDAHGEACVRGQASVPDER
                     FDRLVEIFRPVKVTPASVPFVDINAAGEKAWNTIRQNLSGADGLVHVVDGFSATASGE
                     IAGRYRKLADELILADLLIVENRLERLDRTPAKALRPREALQRDLLPGLKAHLEAGRP
                     LREMNLADEQVLALRSFSFWTIKPELVVINTGEDVAAVEPAALALPAGQPRIRICARL
                     EAEIAALPADERQDYLAAMGITAPAFTQVIRTAFAMLARQVYFTVGEDEVRAWVIPVG
                     AKAPQAAAAIHQDFERGFIKAEVVGYDEFLAHGGTLAGAKAAGRLRLEGKDYVVRDGD
                     IISFRFNV"
     gene            complement(4950..>6029)
                     /locus_tag="PLW16_09520"
     CDS             complement(4950..>6029)
                     /locus_tag="PLW16_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71189.1"
                     /translation="DVYWCLADIGWITGHSYIVYGPLAICASSVIYEGVPNYPDAGRP
                     WRIAQNLDVNIFHTAPTAIRALRKLGPDEPQKYTYHFKHMTTVGEPIEPEVWKWYYYT
                     VGKGEAAICDTWWQTENGGFLCSTLPAIKPMKPGSAGPGVPGIHPIVFDDEGNVVPPG
                     AGKAGNICIQNPWPGSFQTIWRDRERYVNQYYSRYCKNPKSKRWQDWPYMTGDAAVVA
                     ADGYVRILGRIDDVINVSGHRLGTKEIESAAMTVVDVAEAAVVAVKHEVKGKEPDLYV
                     ALKPGCTPSDALAKKIQDAVVQEIGAIARPRKVWIVSDMPKTRSGKIMRRVLSAISNG
                     TDVGDVMTLANPEIVVEITKMVQGK"
BASE COUNT         1013 a         2062 c         1944 g         1010 t
ORIGIN      
        1 ttcttcacct tcctcgtgaa caacgaactc ttccgcctgc ggctcaagct ctcgcgcagc
       61 tcccacattt cgcacgagga attcgccgac gtggaggcgc gcttcctcga gggccggttc
      121 acccccgaga tcctggagca gttcaaggac atgctcgatt acttcggcca ggccccgatc
      181 atcgtccggt cgagctcgct gctcgaggac agcttcggca acgccttcgc cggcaagtac
      241 cggagcgagt actgcgcgaa ccagggaagc ccggaggaac gcctggaggc ctttctcacg
      301 gcggtgaagc tcgtctatgc gagcacgctc aaccccgacg ccctgacgta ccggcggatg
      361 agggggctcg gggagagcga cgagcagatg gcgatcctcg tccagcgggt atccggcatg
      421 cccttcaacc gctacttctt cccgaccctg gccggcgtcg ccttctcccg gaacctctac
      481 gcctggacga gccggatcga tccggcgcag ggcatgatcc ggttggtcct cgggctgggc
      541 acccgggccg tcaaccgcgt gggcgacgac tacccgcgga tgatcgcggt gagccatccg
      601 aaactgcggc ccgagacggg cacggggatt gccaagtatt cccagtggga gatggacgtg
      661 atcgacctgg aggagaaccg gctcgagacg aagtccgtcc gggaggtctt ccgcgacggg
      721 gagtatccga acctgccgat gctcgtctca ctgatgaagg acggctacct ctccgacccc
      781 ttcaccgggc ggctgccgcc ggggacgaac aagttcgtcc tgaccttcaa caacctgatc
      841 aaccggacgg atttcgtgaa gatcgtgggc gagatgctct cccggctcga ggcggcctac
      901 ggccacccgc tggacacgga attcacggcc gccgtggacc ccgagggccg cgtccgggtg
      961 aacctgctcc agtgccgccc catgcgtctg cccggaacga cggggcccgt cgcgctgccc
     1021 gccggcctgc cgaaagagcg ggtcctcttc cgctccggcc ggacgatcag cggcggggtc
     1081 gtggagcgga tccgctacat cctctacatc gatccgaagc ggtacgccac gatctcggat
     1141 gccgacgtca agaagacgtt gggccgggtc gtcggccgga tcaacgagca tcccgggatc
     1201 gccggccggc cgctcgtcat gatggggccg gggcgctggg gaagcagcaa cattgacctc
     1261 ggcgtcaatg tgagctatgc agacatcaac aacacgtcgg tgctggtcga gatcgcccgg
     1321 gaggaggcgg ggcacgtccc cgaggtgtcc tacggaaccc atttcttcct ggacctcgta
     1381 gaggcccgga tcatctatct gcccgtgtac ccgaacgatc cggaggcgga ttttcagcac
     1441 gccttcttcg cggagaccgc caacgccctg acggacctcc tgccggaggc gggccggtac
     1501 gcgccctacg tccacctgat cgacctgccg gcggcgaccg gggggcgctg cgcccgcctc
     1561 gtcgccgatc cggagagcca gcaggcggtg tgctatatcg aataggcgtc gggcgacggg
     1621 cgacgaaacg acgaggaatc gtttccggtg cctgacgccc cgcaaccaag gaggtttcac
     1681 gatgatcgag ttcacgaaga tgagcggcag cggcaacgat ttcatcctga tcgacaaccg
     1741 ggcgcggacg cttcaggttc ccgacatcac ggcctttgtc caagccgtct gcgcgcgaaa
     1801 gatcgccgtc ggggcggacg gcctgatcct gatcgagccc tccgaccggg tcgatttccg
     1861 ctggcggttc ttcaacgccg acggctccga ggtggagatg tgcggaaacg gcggccgctg
     1921 cgccgctcgc ttcgcctttc tcaagggcat cgcgaaggag cggatgtcct tcgagacggg
     1981 ggcgggcgtc atcgacgccg aggtccgggg cgacgtcgtg aagctgcggc tgacggaccc
     2041 ccgggacctg gccatcaacc agagcctttc cgtggacggg aaatcgctcg aggtccacag
     2101 catcaacacc ggcgtcccgc acgtcgtgca cttcgtcaac gacctggagc acaccgacgt
     2161 cttccatctc ggccggatgc ttcgccatca cgcccagtac cagccggcgg gaaccaacgc
     2221 caatttcgtc gccgccatcg atccccacgc cctcgaaatc cggacgtacg agcgcggcgt
     2281 ggaggacgag acgctggcct gcgggacggg cgcggtcgcc tcggccctga tctccgcctg
     2341 gaaggggctc gtcgaatccc cggtcgaggt ccgggtccgg agcggcgaaa ccctttgcat
     2401 tcatttcaca aagaaggcgg ggggattcga gaagatttat cttgaaggcc gggccaaagt
     2461 ggtttatgag ggacgcctgt gggaggaggc atatcgccct taggaggttc cccttgcaga
     2521 ccgccgaccg cttgacgaag atcccgccct acctgttcat ggagctccgc aagaagatca
     2581 accaggccaa ggccgacggc gtggacgtga tcagcctggc gatcggcgac cccgtggagc
     2641 cgacacccgc cccgatcatc gcggagctgt gccggcgggc gcaggactcg gagaaccacc
     2701 gctatccgac ggacgaggaa aagggcatgc tcgccttccg ccgggaggtc gcccgctggt
     2761 acggaacgcg ctacggggtc gacctgaagc cggagacgga gatcctgggc ctgatcgggt
     2821 ccaaggaggg ctgccaccac ttcgtcctcg cccggatcaa tccgggggac gtcgtcctca
     2881 tgaccgatcc cggctatccc gcctaccgcg cgagcatcct gatgggcggg ggcgaaccgt
     2941 acaacgtccc gatcctgagg gagaacggct accttccggt tttcgccgat atcccctccg
     3001 acgtcgcgaa gcgggcccgc gggatgttcc tcaactaccc caacaacccg acgggggcct
     3061 gcgcgactaa acccttcctg aacaacctcg tcgccttcgc gcgccagttc gacatcgccg
     3121 tctgctacga caacccctac agcgagatcc tcttcggggg tcaggaacgg atcagcttcc
     3181 tcatggctgc gggcgcgaag gacgtcggca tcgaactcaa cagcctctcg aagcccttca
     3241 acatgtgcgg ctggcggatc ggcatggccg ccggcaaccc cgacctgatc gccgccatca
     3301 gcaaggtcaa ggagaacacc gattccggga tcttcaaccc gatccagtac gcggcgatcc
     3361 atgccctggc gaagaacgcc ccggccgtgg aggacatgct ggccgtctac gcgcggcggc
     3421 gggagcgggt cctggccgcg ctcgccagga tcggcatcac gttcgatgcg ccgaagggca
     3481 ccttctacct gtgggtcccg gtccccaagg ggatgacttc cctcgagttc acgaaccgcc
     3541 tcttcgacaa gaccgccgtc gtggtggcct ccggcaccgc ctatggccaa tacggcgagg
     3601 gtttcgtccg catttcgctc accgtcccgg acgaccgtct gaccgaggcg atgcgccgca
     3661 tcgagcagga attcgcctga gaacaggcgc tctccgccgt tcgcacgcgc cggaacgccg
     3721 gtatcggatc cggctccgca tcgccctcct tcgcggggtt gaaatcaccc acggggtgtc
     3781 ccatccccac ttgctttcgg tttgcattac acccgtacga cgcgatcccg gcggatgctc
     3841 cgccgtcttt ccgcttgcca aggggcgtac cggctggtat agaggcgcgc gctgggggat
     3901 tggggatgga aatcggaatc gtcggcctgc cgcagagcgg gaagagcacc cttttcgaga
     3961 tcatgaccgg cgtccgggga cgggacgcgc acggggaggc ctgcgtgcgc ggccaggcgt
     4021 ccgtgccgga cgagcggttc gaccggctgg tggagatctt ccgccccgtc aaggtaacgc
     4081 ccgccagcgt ccctttcgtc gatatcaacg ccgcgggcga gaaggcgtgg aacacgatcc
     4141 gccagaacct gagcggcgcc gacggcctcg tccacgtcgt cgacgggttt tccgccaccg
     4201 cgtccggtga gatcgcgggc cgctaccgca agctcgccga cgagctgatc ctggcggacc
     4261 tcctcatcgt cgagaaccgc ctggagcgcc tggaccggac accggcaaag gccctccgcc
     4321 cgcgggaggc cctgcagcgg gacctcctgc ccggtctcaa ggcgcacctg gaagcgggcc
     4381 gccccctccg ggagatgaac ctcgcggacg agcaggttct cgcgctgcgc agcttctcct
     4441 tctggacgat caagccggaa ctcgtcgtga tcaacacggg cgaggacgtg gcggcggtcg
     4501 agcccgccgc cctcgcgctg ccggcgggac agccgcggat ccgcatctgc gcccgcctcg
     4561 aagcggagat cgcggccctg ccggccgacg agcgccagga ctatctggcc gccatgggga
     4621 tcaccgcgcc ggccttcacg caggtcatcc gcaccgcctt cgccatgctc gcgcggcagg
     4681 tgtacttcac cgtcggggag gacgaggtcc gggcctgggt gatccccgtc ggggcgaagg
     4741 cgccgcaggc ggcggcggcc atccaccagg atttcgagcg gggcttcatc aaggccgagg
     4801 tcgtgggcta cgacgagttc ctcgcgcacg gcggcaccct ggccggggcc aaggccgccg
     4861 gccggctccg cctcgaaggc aaggactacg tcgtccggga cggcgacatc atctccttcc
     4921 gcttcaacgt ctgagcgccg cccggaccct cacttcccct ggaccatctt cgtgatctcc
     4981 acgacgatct cggggttcgc cagcgtcatg acgtcgccca cgtccgtccc gttggagatg
     5041 gcgctcagga cgcggcgcat gatctttccg gaccgggtct tcggcatgtc ggagacgatc
     5101 cagacctttc tcggcctcgc gatcgcgccg atttcctgaa cgaccgcgtc ctggatcttc
     5161 ttggccagcg catcgctcgg cgtacagccg ggcttcaggg cgacgtacag atccggctcc
     5221 tttcccttca cctcgtgctt caccgccacg acggccgcct ccgccacatc cacaacggtc
     5281 atggcggccg attcgatctc cttcgtcccc agccggtggc cggaaacgtt gatcacatcg
     5341 tcgatccggc cgaggatgcg gacatagccg tcggcggcga cgaccgcggc gtcgcccgtc
     5401 atgtagggcc agtcctgcca ccgtttgctt ttgggattct tgcagtacct cgaatagtac
     5461 tgattgacgt accgttcgcg gtcgcgccag atggtctgga agctgccggg ccacggattc
     5521 tggatgcaga tgttccccgc ctttccggcg cccgggggga cgacgttgcc ttcgtcgtcg
     5581 aacacgatcg ggtgaatgcc cgggacgccc ggtccggcgc tccccggctt catgggcttg
     5641 atcgccggca gcgtgctgca caagaacccg ccgttttccg tctgccacca ggtgtcgcag
     5701 atggccgcct ctcccttgcc gacggtatag taataccact tccacacctc gggctcgatc
     5761 ggctccccga ccgtggtcat gtgcttgaag tggtaggtgt atttctgcgg ttcgtccggg
     5821 cccagctttc tcagggcgcg gatcgcggtc ggcgccgtat ggaagatgtt cacgtccaga
     5881 ttctgggcga tccgccaggg ccggcccgca tccggatagt tcggcacgcc ctcgtagatg
     5941 accgaggagg cgcagatggc cagcggaccg tagacgatgt aggagtggcc ggtgatccag
     6001 ccgatgtccg ccaggcacca gtagacgtc
//
LOCUS       DAOOLJ010000086         5971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_15878_length_5971_cov_13.844828, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000086 DAOOLJ010000000
VERSION     DAOOLJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15878_length_5971_cov_13.844828"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..439
                     /locus_tag="PLW16_09525"
     CDS             2..439
                     /locus_tag="PLW16_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPB71190.1"
                     /translation="MSENQAPSSWEPKIVAIVCNWCTYAGADLAGISRIQYPPNVRII
                     RVPCTGRINPFYVVKALQAGADGVLVSGCHPGECHYLTGNLSARRKFATLKRFLSYIG
                     VEGDRTMFTWVSASEGERFSQVIKGVTEAVRALGPARNLVKQL"
     gene            507..1526
                     /locus_tag="PLW16_09530"
     CDS             507..1526
                     /locus_tag="PLW16_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB71191.1"
                     /translation="MENVEKKLREEAKLLLAEKKVDVLVGYEAGTLPMTATPCFITAP
                     EEADRLVWNAFCVQNLAKFVQDLISLHRQSQQRVKPEARTRKVVGVVARGCTTRSLVI
                     QLQERQFNRDDIFVVGVPCGGYVDKCKVAARVAGQEIREASLVGDQLSVKTAGEAKTL
                     PLGEILADNCLTCRFNNPVISDLMIGSPAPAMAPESEYAGVEAHEKLSLAERWSYFEK
                     EMAKCIRCNACRQVCPSCYCPVCFAEQSQPQWVGISEDETDTQVFQIMRLYHMVGRCV
                     DCGSCVAVCPMGVDLRTFLKKLDKDVFELFGNRAGSSLEDLPPLSQFKEDDREDFIYD
                     PQKAK"
     gene            1561..2085
                     /locus_tag="PLW16_09535"
     CDS             1561..2085
                     /locus_tag="PLW16_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB71192.1"
                     /translation="MKTFLEEVNDMIGGVPIQRCYHCRKCTAGCPLAFAMEYNPNRII
                     KMIQMGMKEEVLGSSTIWMCASCETCVTRCPNQVDIARMMDALRQMSIESGRPAKEAN
                     VLKFHKAFLANIRLGGRINEPIMMVQYKLASGDLFSDMAMGVGMFMKGKLSLLSPRTR
                     DMKAVRDIFAKTKP"
     gene            2112..2960
                     /locus_tag="PLW16_09540"
     CDS             2112..2960
                     /locus_tag="PLW16_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HPB71193.1"
                     /translation="MKVSYYPGCSLHATGKEYGQSVRAVSKALGIELKEIDDWSCCGA
                     TSAHSTNFKLSIALPARNLIAAERDAMDVMVPCAACFNRFKTAQHHLDKDKALKSEIE
                     VIVGAKYQGGIRVRHPLDIISNDVGLDTLAGKAVHKLTGLKPVSYYGCLLLRPPEVCE
                     FDDYENPILMDGILGALGAEVRPWSYKTDCCGGSLTISKTGIVVKMVDRLMTMAREAE
                     ANCLVTACPVCMANLDMRASEHVRLPVFYFTELMALAMGLDGPEGWFKLHNIDPRPLL
                     TGLGLV"
     gene            3084..4106
                     /locus_tag="PLW16_09545"
     CDS             3084..4106
                     /locus_tag="PLW16_09545"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB71194.1"
                     /translation="MEKRYIKKDSLNGRVKKMAEAGLVYAPVKDGDNVLFQVLTPGME
                     PLTAYANSKNAPKNFFFPHTEKLMKYTRTEKGMAFSGELAEAREAVLFGVRPCDARSF
                     VLLDMLFDQEKYRDPYYVARREKTTVISLACIYPPYTTCFCTSVAGGPMDAAGADILL
                     TDLGERYLVEFLTPKGEKLEAHFAGDKAGDEADAQKQAIAAQAEQEITSRIPAKEIKP
                     ILDVSFEHPFWNTIHQKCLACGTCTYLCPTCHCFDISDEQKGDDGIRLRNWDSCMFPL
                     FTLETSGHNPRPTQKERWRQRAMHKFKYYVDNFNAIACVGCGRCVMYCPVNIDIRKIV
                     TDISKL"
     gene            4122..4961
                     /locus_tag="PLW16_09550"
     CDS             4122..4961
                     /locus_tag="PLW16_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPB71195.1"
                     /translation="MQNPYLPIPVVVSKIITEVETKDIKTFRLEFLNKEDEANFKYLP
                     GQFAELSLYGKGESPIGIASSPTQPGYIEFTVQKAGVVTSSLHEIEEGTHMGVRGPLG
                     NSWPIEYLEGKNIVVVGGGFAFTTLRSLINYMIHDDNRKRFGSITVIYGARTPGLLIY
                     KDELAEWQKRSDINLNVTVDKGDASWKGREGFVPTVCKEVAPSSENAVTVICGPPVMI
                     RFTLPVFFDLGFSKENIVTSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFTLAELDKLT
                     KEY"
     gene            complement(5105..5716)
                     /gene="lepB"
                     /locus_tag="PLW16_09555"
     CDS             complement(5105..5716)
                     /gene="lepB"
                     /locus_tag="PLW16_09555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB71196.1"
                     /translation="MTKEKSKIQEYVEAIILAILIAFFIRTFVIQAFKIPSGSMKPTL
                     LIGDHILVNKFIYGVKVPYLRSTILPFREPKRGDIVVFIYPEDRSKDFIKRVVGVAGD
                     TLEIRNKKIFLNGLPYDDKHGVYVDDFIIPGAIQPRDNFAPVTVPKESLFVMGDNRDQ
                     SYDSRFWGFVEQKDVMGKAMVIYWSWNREAFGVRWSRLGKILR"
     gene            complement(5726..>5971)
                     /locus_tag="PLW16_09560"
     CDS             complement(5726..>5971)
                     /locus_tag="PLW16_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HPB71197.1"
                     /translation="GVGGVVGMMVLTPWLYGPGALIGLFAGAFTGVWITEMIRQLNLK
                     SAFRAEPRAILGWTAGSLVRGALTVGMTVVILITIYS"
BASE COUNT         1312 a         1702 c         1804 g         1153 t
ORIGIN      
        1 tatgtctgag aatcaagcac cgtccagctg ggaaccgaag atcgtcgcca ttgtctgcaa
       61 ctggtgcacc tatgcgggtg cggatctggc cgggatcagc cgcatccagt atcccccgaa
      121 cgtccggatc atccgtgttc cgtgtacggg gaggatcaat ccgttctacg tcgtcaaggc
      181 cctgcaggct ggggcggacg gggttcttgt ttcggggtgc catccgggcg aatgtcacta
      241 tctgacggga aacctctccg cgaggcggaa atttgccacc ctcaagcgct tcctgagcta
      301 tatcggcgtg gagggggacc ggacgatgtt cacctgggtt tccgcttccg agggggagcg
      361 tttctcccag gtcatcaaag gggtgaccga ggccgtccgg gccctggggc cggcccggaa
      421 tttggtcaag cagctctagg aaaaacgcgg ggtcgagcca acccgccttg gatcatgaac
      481 tccataccag tgaggaaatc aaaacggtgg aaaatgtaga gaagaaactc cgggaagaag
      541 cgaagctgct gctggcggaa aagaaggtcg acgtcctcgt gggatacgaa gcgggcacgc
      601 tgcccatgac ggccaccccc tgcttcatca ccgcgccgga ggaggcggac cggctcgtgt
      661 ggaacgcctt ctgcgttcag aacttggcca agttcgtcca ggatctgatc agccttcaca
      721 ggcagtcgca gcagcgcgtc aaaccggagg cgcggacccg aaaggtcgtc ggtgtggttg
      781 cccggggatg cacgacacgc tccctggtga tccagcttca ggagcggcag ttcaaccggg
      841 acgatatctt cgtcgtcggc gtcccctgcg gcggctatgt ggacaagtgt aaggtcgcgg
      901 cgcgggtggc gggtcaggag atccgggagg cgtctctcgt tggcgatcaa ctctccgtca
      961 aaacggccgg agaggcgaaa acccttcccc ttggcgagat tttggccgac aattgcctga
     1021 cgtgccgctt caacaacccg gtcatctcag acctcatgat cggaagcccg gccccggcga
     1081 tggcgcccga gtccgagtat gccggcgtcg aagcgcacga aaagctgtcg ctggcggagc
     1141 gatggagcta tttcgagaag gaaatggcca agtgcatccg atgcaacgcc tgccggcagg
     1201 tctgcccgtc ctgctattgc ccggtctgct tcgcggagca gagccagccg cagtgggtcg
     1261 gtatcagcga agatgaaacc gatacgcagg tcttccagat catgcgcctc tatcacatgg
     1321 tcgggcgttg cgtggattgc gggtcgtgcg tcgcggtctg tcccatgggc gtggatttaa
     1381 ggacgttcct gaaaaagctc gacaaggacg tcttcgaatt gttcggcaat cgggcgggat
     1441 cgtcgctgga ggatctgccg cccctgtccc agttcaagga agatgatcgg gaggatttta
     1501 tctacgatcc tcagaaggcg aaataatctt caagaccaaa catccagtca cggagtcggc
     1561 atgaaaacct ttttggaaga agtcaatgac atgatcggcg gagttcccat ccagcgttgc
     1621 tatcattgcc gcaaatgcac ggcgggctgc cccctggcgt ttgccatgga gtacaacccc
     1681 aaccggatca tcaagatgat ccagatgggg atgaaggaag aagtactcgg gagttcgacg
     1741 atctggatgt gcgcatcgtg cgaaacctgc gtcacgcgct gtccgaacca ggtggacatc
     1801 gcgcggatga tggatgcgct ccggcagatg agcatcgaat cgggccgccc cgcgaaggag
     1861 gcgaacgtcc tgaagttcca caaagcgttt ctcgccaaca tccgccttgg cggtaggatc
     1921 aacgagccca tcatgatggt ccagtacaaa ctggcttccg gagatctctt ttccgacatg
     1981 gccatgggcg tgggcatgtt catgaagggc aagctctccc tgctctcccc caggacccgg
     2041 gacatgaagg ccgtgaggga catcttcgcg aaaacgaagc cgtaaaggac tgcttttaag
     2101 gaggcacgct tttgaaagtt tcatactatc ccggatgctc gcttcatgcc acgggcaagg
     2161 aatacggcca gtcggtccgg gcggtcagca aggcgctcgg gatcgaactc aaggaaatcg
     2221 atgactggtc gtgctgcggg gcgacatcgg cccacagtac caacttcaaa ctttccatcg
     2281 ccctgccggc gaggaacctg atcgccgcgg aacgggacgc catggacgtc atggtcccgt
     2341 gtgccgcgtg tttcaaccgc ttcaaaaccg cacagcatca cctggacaag gacaaggcgc
     2401 tgaaatccga aatcgaggtc atcgtcgggg caaagtatca gggcgggatc cgggttcggc
     2461 atcccctcga tatcatctcg aacgatgtcg gtctcgatac actggctgga aaggcggtcc
     2521 acaaactaac gggactcaag cctgtctctt attacggctg cctcctgctc cggccaccgg
     2581 aggtgtgcga gttcgacgat tacgagaatc ccatcctgat ggacggcatt ctcggcgccc
     2641 tcggggcgga agtccggccc tggtcctaca agacggactg ctgcggcggg agtctgacca
     2701 tcagtaagac gggcatcgtt gtcaagatgg tcgacaggct catgaccatg gcgagggagg
     2761 cggaagccaa ctgcctcgtg accgcctgtc cggtctgcat ggccaatctc gacatgcggg
     2821 cctcagagca tgtccggctg ccggtgtttt attttacgga gctgatggcc ctggcgatgg
     2881 ggctcgatgg gccggaagga tggttcaagc tgcacaacat cgatccgcgg cccctgttga
     2941 ccggactggg actggtctaa ccggtaaagc cggagcggga cgtcgggagc ggcatcccgg
     3001 gcgggtatgg ggcgggaacg gccccctgaa gaagacgaaa cgcttcccct cgcgggggag
     3061 aaaaaacgca ggcaaggacg gatatggaaa aacggtacat caagaaagac agcctgaacg
     3121 gcagggtcaa gaagatggcg gaggcgggac tggtttacgc cccggtgaag gatggggata
     3181 acgtcctttt ccaggttctg acgccgggca tggagccgct gacggcgtat gccaacagca
     3241 agaacgcccc gaaaaacttc ttcttcccgc acacggaaaa gctgatgaag tacacgcgga
     3301 cggaaaaggg catggcgttt tccggcgaac tggcggaggc gagggaggcc gtcctgttcg
     3361 gtgtccgccc ctgcgatgcc cgcagtttcg tccttctcga catgctcttc gatcaggaaa
     3421 aataccgtga cccctactat gttgcccggc gggaaaagac caccgtcatt tccttggcct
     3481 gtatctaccc cccctatacc acctgtttct gcacctccgt ggccggcggg ccaatggacg
     3541 ccgcgggcgc agatatcctc ctgacggatc tcggggaacg ctatctggtc gagttcctca
     3601 cccccaaggg ggagaagctg gaggcccatt tcgcgggtga caaggcgggc gatgaggcgg
     3661 acgcccagaa gcaggccatc gcagcccagg ccgaacagga aatcacctcc cgcatcccgg
     3721 ccaaagagat caagccgatc ctggacgtca gtttcgagca tccgttctgg aacacgattc
     3781 atcagaaatg tctggcttgc gggacctgca cctacctctg ccccacctgt cactgcttcg
     3841 acatcagcga cgagcagaag ggcgacgacg gaatccgtct gcgcaactgg gattcctgca
     3901 tgttccccct gttcacgctc gagacatccg gacacaatcc gcggccgacg cagaaggagc
     3961 gctggcgtca gcgtgccatg cacaagttca aatattacgt ggacaatttc aacgccatcg
     4021 cctgcgtagg atgcggccgt tgcgtgatgt actgtccggt gaacatcgat atcagaaaaa
     4081 tcgtcacgga tatttcgaaa ttatagaatc gggagcgtgt aatgcagaat ccatatttac
     4141 ccattccggt cgtcgtctcg aaaatcatca cggaagtgga aacgaaggac atcaagacct
     4201 tccggctgga gtttctcaac aaggaagacg aggccaactt caagtatctg ccgggtcagt
     4261 ttgccgaact ctccctctac ggcaaggggg aatcccctat cgggatcgct tcatcgccga
     4321 cgcagccggg ttatatcgag tttaccgtcc agaaggcggg cgtggtgacc tccagccttc
     4381 acgagatcga ggaagggacc catatggggg tccgcggacc cctcgggaat tcctggccca
     4441 tcgaatacct ggaaggaaag aacatcgtgg tcgtcggtgg cggctttgcc ttcacgacgc
     4501 ttcgctccct catcaactac atgatccacg acgacaaccg gaagcgcttt gggagtatca
     4561 ccgtcatcta cggcgcgcgg accccggggc tcctcatcta caaggatgaa ctggcagaat
     4621 ggcagaaacg gtccgacatc aacctgaacg tcaccgtcga caagggagac gcctcctgga
     4681 agggacggga gggctttgtc cccaccgtct gcaaggaagt ggcgcccagc tccgagaatg
     4741 cggtgaccgt catatgcggc ccgccggtca tgatccgctt taccctgccg gtctttttcg
     4801 atctcggttt ttccaaggag aacatcgtca cctccctcga aatgcggatg aaatgcggca
     4861 tcggcaaatg cggccgctgc aacgtcggca gcaaatatgt ctgcaaggac ggaccggtct
     4921 tcacgcttgc ggagctggac aaactgacga aggagtactg aggttcgcgt ctgaaaagag
     4981 gtgcggaagg gcgagcccct caggggtgca cttacagatc ccgcggcgga atgaaaggga
     5041 aaaagggagt cggacgactc cctttttttg ttgggtttac atctctaacc gggtgacgac
     5101 gccgtcaacg cagaatcttc cccagacggc tccagcgaac accgaacgcc tcgcggttcc
     5161 aggaccagta gatgaccatg gcctttccca taacatcctt ctgttctacg aatccccaaa
     5221 accggctatc atagctctga tcccggttgt cgcccatgac aaagagcgat tccttcggaa
     5281 cggtgacggg tgcgaaattg tcccggggct ggatggcgcc gggaatgatg aaatcgtcca
     5341 catagacgcc gtgcttgtcg tcgtagggga ggccgttcag gaatatcttc ttgttccgga
     5401 tctccagggt atccccggcc acgccgacca cccgcttgat gaaatccttg gagcggtcct
     5461 ccggatagat aaacacgacg atatcccccc gcttgggttc gcgaaacggc aggatcgtac
     5521 tgcgcaggta aggcaccttc accccataga tgaacttgtt aacgaggata tgatcgccga
     5581 tgagcagggt gggtttcatg gagcccgaag ggattttgaa ggcctggatg acgaacgtgc
     5641 ggatgaagaa ggcaatcagg atggcaagga tgatggcttc gacatattcc tggattttcg
     5701 atttttcttt cgtcatggtt tttccttatg aatagatcgt gatcaggatc acgaccgtca
     5761 ttccgacggt cagcgcacct ctgaccaggc ttccggccgt ccatcccaga atggcgcgcg
     5821 gttcggcgcg aaaggcggat ttgaggttca actgacggat catctcggtg atccagacgc
     5881 cggtaaaggc ccccgcgaac aatccgatca gggcgccggg gccgtagagc cagggggtca
     5941 ggaccatcat gcccacgacg cccccaactc c
//
LOCUS       DAOOLJ010000087         5930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_16039_length_5930_cov_18.841532, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000087 DAOOLJ010000000
VERSION     DAOOLJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16039_length_5930_cov_18.841532"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PLW16_09565"
     CDS             complement(<1..1368)
                     /locus_tag="PLW16_09565"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPB71198.1"
                     /translation="MKRKTGARKIPQLTPNALTVLKRRYLKRDQEGTVLESPDDLFRR
                     VAESLATADRRFRGKSDVAKLADAFYRMMTDLEFLPNSPTLMNAGRELGQLSACFVLP
                     VGDSMEEIFDAVKFTALIHKSGGGTGFSFSRLRPAQDVVMTTTGISSGPISFMRVFDV
                     ATETIKQGGTRRGANMAILRVDHPDIMDFIMCKADKKQLNNFNISVGLTEAFMVAVEN
                     DENYELVNPRNNEVTGTLNARKVFHRIVTQAWENGEPGMIFLDRLNRDNPTPHIGEIE
                     STNPCGEQPLLPYESCNLGSINLGKMVEDGKIDWARLKEVVHLAVHFLDNVVEINRYP
                     LPQIEAMTSGNRKIGLGVMGWADMLILLGIPYDTEEGVRTAEKVMKFIREEGRQASRE
                     LAKRRGAFPNFKGSVFDTPGEPPIRNATITTIAPTGTISIIANASSGVEPIFAVSYVR
                     QVMDND"
     gene            complement(1548..1892)
                     /locus_tag="PLW16_09570"
     CDS             complement(1548..1892)
                     /locus_tag="PLW16_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPB71199.1"
                     /translation="MWRDDAHLLDIVVAAKWALRFVKGMTWKDFDASQLHQDAVVDRL
                     VIISNAAKKVSREMKEAHPEIPWREIIAVSNNRLIFDYFRVDIEAVWRTIQDDLPGIV
                     QALEPLVPPEKA"
     gene            complement(1879..2193)
                     /locus_tag="PLW16_09575"
     CDS             complement(1879..2193)
                     /locus_tag="PLW16_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPB71200.1"
                     /translation="MAGVALPPPEVLAAFCQKWRIVELSLFGSVLREDFRPESDVDVL
                     VRFAEDVKYGMFDFVHLQEELTALVGRPVDAVDWRAVERNENYLRREHILKHREVLYV
                     AG"
     gene            complement(2223..3974)
                     /locus_tag="PLW16_09580"
     CDS             complement(2223..3974)
                     /locus_tag="PLW16_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71201.1"
                     /translation="MSARNLSIQAGLDGAVIAASWEALDRERVVARLWRKDHTLWKPA
                     PTEIANRLGWLTSPRDMLAELPEIHARVHEIRAAGYTRAVLLGMGGSSLAPEVFRKTF
                     GIREGFLDLTVQDSTDPGAVLALAEGLDPVQTLFIVATKSGGTVETFSLMKFFYRRLV
                     DALGPPEAGRHFIAITDPESALAEMAARFGFRCTFLNNPDIGGRYSALSCFGLVPAAL
                     IGLDVRRFLERAVAQVDTEREGPGKGFGAGTPAARAGDAALPLNGALIGAALGGLAVA
                     GRDKLTFFFSPPLAAFGDWLEQLIAESTGKEGKGILPIVGETPADPAAYGPDRVFALM
                     GFPGDRVLRRQAAGLAAAGHPVIRVDMVEADDLGAQCFLWEVATAVAGWHLGINPFDQ
                     PDVESAKVLARERLAAFRAQGRLPKEAAGIEAGEVRVYGDLQAKTPGAALAAFLDETG
                     PGGYVVLQAYVRPAPETDRALLALRTAIRDRYRLAVASGYGPRFLHSTGQLHKGDAGR
                     GRFIQITADDPRDAVIPDGMDGPEGAVTFGTLKRAQAAGDGEALRKAGRPVIRFHFNR
                     DAARGLAGLAAGLRGTT"
     gene            4215..5003
                     /locus_tag="PLW16_09585"
     CDS             4215..5003
                     /locus_tag="PLW16_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71202.1"
                     /translation="MERIIVQLYEIQEPREAECLLELGVDHIGSVILSEEAWKSPALK
                     DVVRLVKDSPAKSSLIPLMKNADNILRALDYYQPQLVHFCEAIPLAPEDGARRARVRA
                     DLIRTQQRVKKEFPPFGIIRSIPIPAPGLADPPPIREVVLELARTLAPHSDFFLTDTL
                     RGFEDSEDAQPVAGYVGITGEVCDWTLAAALVEASPIPVILAGGISPENVHDGIRRVR
                     PAGIDSCTQTNARDTRTGRPIRFRKDFDRVRRLIDEVRRADAAA"
     gene            complement(5229..5705)
                     /locus_tag="PLW16_09590"
     CDS             complement(5229..5705)
                     /locus_tag="PLW16_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB71203.1"
                     /translation="MPILSLSTETLHILRKPAIYPPHRLYEALNKLPSKRNVVILDSC
                     FSGAGNRSVIAKGLRPLVVMAANQEIVGANTTILSASAGNQVSSTYLEKGHGLMTYFI
                     LKGIKEEPIRNQGGHMDMEKLYPYVKQNVERIARKLYNNEQTPQLWIGKQKSGEGK"
     gene            complement(5626..>5930)
                     /locus_tag="PLW16_09595"
     CDS             complement(5626..>5930)
                     /locus_tag="PLW16_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB71204.1"
                     /translation="PKAEYAANDARLVAEYFTKSLGVPEENLIILTNDKATKSDLEKY
                     FHKWLPNNVSKESDVFIYFSGHGAPNPSGGDAYLVPFDGDPTYITETGYLSASPAL"
BASE COUNT         1056 a         1881 c         1850 g         1143 t
ORIGIN      
        1 gtcgttgtcc atgacctgcc ggacgtagga gacggcgaag atcggctcca cgccggacga
       61 ggcgttggcg atgatcgaaa tggtgccggt cggtgcgatc gtcgtgatgg tggcgttccg
      121 gataggcggc tcgcccggcg tgtcgaagac cgatcccttg aaattgggga aggcgccgcg
      181 tcgcttcgcc agttcccggg aggcctggcg gccctcctcc cggataaatt tcatgacttt
      241 ctccgcggtc cgcacgccct cttcggtgtc gtagggaatc cccaggagga tcagcatatc
      301 ggcccagccc atgacgccca ggccgatctt gcggttgccg gaagtcatgg cctcgatctg
      361 gggcagcggg tagcggttga tctccacgac gttgtcgaga aaatgcacgg ccaggtgcac
      421 gacttcctta agccgggccc agtcgatttt cccgtcttcg accattttcc ccaggttgat
      481 cgagcccaga ttgcaggact cgtagggcag gagcggctgt tccccgcagg ggttcgtcga
      541 ctcgatctct ccgatgtggg gcgtcgggtt gtcgcggttc aggcggtcca gaaaaatcat
      601 gcccggctcg ccgttttccc aggcctgggt gacgatccgg tggaagacct tccgggcgtt
      661 gagcgtcccg gtgacctcgt tgttccgggg gttgacaagt tcgtagttct cgtcgttttc
      721 gacggcgacc atgaaggcct ccgtcaggcc gaccgagatg ttgaagttgt tcaactgctt
      781 cttgtcggcc ttgcacatga tgaagtccat gatgtccgga tgatcgaccc gcaggatcgc
      841 catgttggcg ccgcgccgcg tgccgccctg cttgatggtt tctgtcgcga cgtcgaagac
      901 ccgcatgaac gagatcgggc cgctggaaat cccggtcgtg gtcatcacga cgtcctgggc
      961 cggccgcagg cgggagaagg agaagcccgt gccgccgccc gacttgtgga tcagagcggt
     1021 gaatttgacg gcgtcgaaaa tctcctccat ggaatcgccg acgggcagga cgaaacaggc
     1081 ggaaagctgg cccaattccc ggccggcgtt catcagggtc ggtgaattgg ggagaaactc
     1141 gagatccgtc atcatccggt agaacgcgtc cgcgagcttg gcgacgtcgg acttgccccg
     1201 gaaccggcga tcggcggtgg ccagggactc ggcgacgcgc cggaagaggt cgtcggggct
     1261 ctccagcacc gtgccttcct gatcccgctt gagatagcgc cgtttgagaa cggtcagggc
     1321 attgggggtc agttgcggga tcttccgtgc gccggtcttt cttttcatgc cgatggactc
     1381 ctcaagagat cgaaaaaaat caaagccttc cccctgccga gggggcgccg gccccttgtg
     1441 cgtgaggcac aagatgtagt gtttaggaat ctctttgacc acaagatgtg ggagatgtca
     1501 acaagagaat gggaaaaaat cgcagggggg atgggaaaaa aatgccctta agccttttcc
     1561 ggcgggacga ggggttcgag ggcctggacg atgcccggca ggtcgtcctg gatcgtccgc
     1621 cagaccgctt cgatgtccac gcggaaataa tcgaagatca ggcggttatt gctcacggca
     1681 atgatctccc gccaggggat ctccggatgg gcctccttca tctcccgtga aaccttcttc
     1741 gccgcgttgc tgatgatcac gagccggtcc acgacggcgt cctggtggag ctggctcgcg
     1801 tcgaaatcct tccaggtcat gcccttcacg aaccggagcg cccatttggc cgccacgacg
     1861 atatccagca ggtgggcgtc atcccgccac ataaaggacc tcccgatgct tcaaaatgtg
     1921 ttcccggcgc aggtagttct cgttccgttc cacggccctc cagtccacgg catcgaccgg
     1981 ccgcccgacg agggcggtca gctcctcctg aaggtggacg aaatcgaaca taccatactt
     2041 gacgtcttcc gcaaagcgga caaggacatc gacgtcgctc tccggccgaa aatcctcgcg
     2101 cagaacggac ccgaagaggg agagctcgac gatgcgccat ttctggcaga aggcggcaag
     2161 cacctccggc gggggcaggg cgaccccggc cattcctctt ccctttgggt cggtcctgac
     2221 ggtcatgtcg ttccccgcag accggcggcc agaccggcga gcccccgggc ggcgtccctg
     2281 ttgaaatgaa agcggatcac cggccgcccc gccttccgga gggcctcgcc gtcgcccgcc
     2341 gcctgggcgc gcttcagcgt cccgaaggtg actgcccctt ccggtccgtc catcccgtca
     2401 gggatgaccg catcgcgggg atcgtccgcc gtgatctgga tgaatcgccc ccgccccgcg
     2461 tcccccttgt ggagctgtcc cgtcgagtgg aggaatcggg ggccgtaccc cgacgcgacg
     2521 gccaggcgat aacggtcgcg gatggccgtc cgcagggcga gcagggcccg atccgtctcc
     2581 ggcgcgggcc ggacgtacgc ctgcaggacg acgtagcccc ccggaccggt ttcatccaga
     2641 aacgccgcca gggccgcgcc cggtgttttt gcctgcaaat ccccgtaaac gcgaacctcc
     2701 cccgcctcga tacccgccgc ttccttgggt agccgtccct gcgcccggaa ggcggcgagg
     2761 cgttcgcggg cgaggacctt cgcggactcg acgtcgggct gatcgaaggg gttgattccc
     2821 aggtgccatc cggccacagc cgtcgcgacc tcccagagga agcactgggc gcccagatcg
     2881 tcggcttcga ccatgtccac gcggatgacg gggtgtccgg ccgccgcgag gcccgccgcc
     2941 tgccgccgca ggacccggtc gcccgggaac cccataagcg caaacacgcg gtccgggcca
     3001 tatgcggccg gatcggccgg ggtttcgccc acgatgggga ggatgccctt tccctccttg
     3061 cccgtgctct ccgcgatgag ctgttccagc cagtcgccga aggcggcgag cggcggcgag
     3121 aagaagaaag tcaacttgtc ccggcccgct acggccagtc cacccagggc cgctccgatc
     3181 agcgccccgt tcagcgggag cgccgcgtcc ccggcccgcg ccgcaggggt ccccgcgccg
     3241 aatccttttc ctggcccctc ccgctccgtg tccacctgtg cgacggcccg ctccaggaag
     3301 cgccggacgt cgaggccaat cagggcggcc ggcaccaggc cgaagcagga cagcgccgaa
     3361 taccggccgc cgatgtccgg gttgttgagg aaggtgcagc ggaagccgaa ccgggcggcc
     3421 atctcggcca gcgcgctctc cgggtccgtg atggcgatga aatgccggcc cgcctccggg
     3481 ggaccgagcg cgtcgaccag gcgccggtag aagaacttca tgagggagaa ggtctccacc
     3541 gttccgcccg acttcgtggc gacgatgaag agggtttgga cgggatcgag gccctcggcc
     3601 aaggccagga cggcgcccgg atcggtgctg tcctggacgg tgagatcgag gaacccctcg
     3661 cggatgccga aggttttccg gaagacctcg ggagccaggc tcgatccgcc catgcccagg
     3721 aggacggccc gggtgtagcc cgcggcccgg atttcgtgaa cgcgggcgtg gatctccggg
     3781 agttcggcca gcatgtcccg ggggctggtg agccacccca ggcggttggc gatctccgtc
     3841 ggcgcaggct tccagagggt gtggtccttc cgccagagcc gcgccaccac gcgctcccgg
     3901 tccagggcct cccaggacgc cgcaatgaca gccccgtcga gccctgcttg tatcgacagg
     3961 ttccgtgccg acatcgcccc acctccctta ccggtcgttc tccggaatcg acggtcggag
     4021 gccccgtccc gcggtgcggt cggggcatac cgaaacccga ccgatccggc cgtcccaacc
     4081 cggtgcgcgc cgcgggttct cccgggaccg atcacccact gtcctgtcat gaatagcgaa
     4141 aaagacagcg gaaggcaagt cccgcgtctg ccgagccctt gacggatgcg gggttttcct
     4201 ctacaaggcg gaccatggaa cggatcatcg tgcagcttta cgaaatccag gagccccgcg
     4261 aggcggagtg cctgctggag ctgggggtgg atcacatcgg cagcgtgatc ctctcggagg
     4321 aggcctggaa atcgcccgcc ctcaaggacg tcgtccgcct ggtcaaggac tccccggcga
     4381 agagcagcct gatcccgctc atgaagaacg ccgataacat cctccgcgct ttggactact
     4441 accagccgca gctcgtgcat ttctgcgagg cgatcccgct tgcgcccgaa gacggcgccc
     4501 ggcgcgcccg cgtccgcgcg gatctgatcc gaacccagca gcgggtgaaa aaggagtttc
     4561 cccccttcgg gattatccgc tccatcccga ttcccgcccc ggggcttgcc gatccgccac
     4621 cgatccggga ggtggtgctc gagctcgccc gcaccctggc gccgcacagc gatttcttcc
     4681 taaccgatac gctgcgagga tttgaggatt ccgaggacgc ccagcccgtg gcgggctacg
     4741 tgggcatcac cggggaggtc tgcgactgga cgctcgcggc ggccctcgtt gaggcgagcc
     4801 cgatccccgt gatcttggct ggggggatct cgcccgagaa cgtccacgac ggaatccggc
     4861 gggtcaggcc cgccgggatc gacagttgca cccagaccaa cgcccgcgac acgcgcaccg
     4921 gccgccccat ccgcttccgg aaggacttcg accgcgttcg ccgcctgatc gacgaggtcc
     4981 gccgcgccga tgcggccgcc tgacccctcg tccatcaaag tcaggcaccg ctgtattgct
     5041 gagaatcaac gataaccgct tgaagtctgc atcgttgcca acagagagta aattgcacct
     5101 tgttatgctg tccagttatt gggagagttt aatcgcctgc atgagagagc ctttttcggc
     5161 tcttattttc tcgctgtatt ttctggatgg tttccggatg gaaaccagat ggccatgtca
     5221 tggtgttctt actttccctc tccggatttt tgctttccaa tccataattg gggcgtttgt
     5281 tcgttgttgt acagttttct ggcaatgcgt tccacatttt gtttcacata gggataaagt
     5341 ttttccatgt ccatatggcc accctgattc cggattggtt cctctttaat ccccttcaag
     5401 ataaaatagg tcatcaaacc atgtcctttt tccagatatg tcgaactgac ctggttgccg
     5461 gcggatgcag acagaattgt ggtattcgct ccgacgatct cctgatttgc agccatgacg
     5521 acaagcggtc tcaacccttt ggctataact gatcgattgc cggcgcccga aaaacaggaa
     5581 tccaagatta ccacgtttct ttttgatggc aatttgttca gggcttcata aagccggtga
     5641 ggcggataga tagccggttt ccgtaatata tgtagggtct ccgtcgaaag ggacaagata
     5701 ggcatcgcca cccgaaggat taggcgcgcc gtgtccggag aaatagataa aaacatcgct
     5761 ttctttagag acgttgttgg gcagccattt gtggaaatat ttttccaaat cgctctttgt
     5821 cgctttgtcg ttggtcagaa ttattagatt ttcctcagga acgccgagtg attttgtaaa
     5881 atattcagcg accagacgag catcgtttgc ggcgtattcc gccttaggta
//
LOCUS       DAOOLJ010000088         5914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_16094_length_5914_cov_14.888206, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000088 DAOOLJ010000000
VERSION     DAOOLJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16094_length_5914_cov_14.888206"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLW16_09600"
     CDS             complement(<1..227)
                     /locus_tag="PLW16_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB71205.1"
                     /translation="MFPSVMKSPRSVDIEITSFCNLRCAYCSHFSSSGDVDTDLPLSA
                     WLRFFDELGRCAVMTVTLSGGEPFMRPDLKE"
     gene            complement(235..522)
                     /gene="scmD"
                     /locus_tag="PLW16_09605"
     CDS             complement(235..522)
                     /gene="scmD"
                     /locus_tag="PLW16_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SynChlorMet cassette protein ScmD"
                     /protein_id="HPB71206.1"
                     /translation="MEPASETLIMANPLVVLREEFDDWAILFDPDTGSAFGINPIGVM
                     VWKLLDGCHSLEKITDIIREAAEEVPEDVAEHIRDFVRATVEFGLAGPKAA"
     gene            complement(601..1386)
                     /locus_tag="PLW16_09610"
     CDS             complement(601..1386)
                     /locus_tag="PLW16_09610"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB71207.1"
                     /translation="MIEAIIEDAPLVDELYKLTAEGGHNDDLDIVSPVNSMIYSLKVG
                     KRMGYSAFKLTEVCMAALHHDIGMVFVDDAISRKEGRLTEEEFTELKKHTAYGRDILK
                     ALDETYPQVSRAVYEHHERENGRGYPLGLKGEEICEYAKIIAICDSYEAMTHDRAYRK
                     AGAQHKSILEIAQAKDALFSPHIIKIFLDEITMYPIGSYVLLNNKAIGIVVKTNPTNP
                     LKPVINLVMDGHGKRLLEDRRIDLADNNILNIQGSILKDEIYQ"
     gene            1925..3082
                     /locus_tag="PLW16_09615"
     CDS             1925..3082
                     /locus_tag="PLW16_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPB71208.1"
                     /translation="MNFRIMRVFSSVILALCSLVILSCTLEYPGIPVKTYQEPATVLE
                     ARDREQQEMAETIRGLSDVTENSVFTEKYGTPEYRVGSGDVITINYWVPSPDEGFKQQ
                     TYTATVRPDGKISFVYADDIFVEGMTAREIDDTLTRLSQKYLRDPRIEVMVKEFKSKS
                     ILIFGRINIMDQQAGVSGPGRYPLVGKTRVLDVIITAGGPVAGQDTGNANLRQVELLR
                     QGKRYFLNIYNAFIGGDAIDNVVVEHGDVINVPEMPTYAERIYVFGQVRSQGILRLRD
                     SNDLLSAIAISGGTTPVAVKTDIKIIRDYDAQKRGKPIILSANLDQILMQGDLAQNIP
                     LHDGDVIYVPRSVIGDINEFIENITPMLDLIQNRPAEFRTNYFKDQTKLRW"
     gene            3102..>5914
                     /locus_tag="PLW16_09620"
     CDS             3102..>5914
                     /locus_tag="PLW16_09620"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPB71209.1"
                     /translation="MPMTYDLNLREYWRTIRRRKFIIVFTVVMMTLFSFFFSIMGRPT
                     PIYKTSASVKVEKSGSVTGLYIRAVSWSTTDYMETQMAMIKSYLIMEMVAKKLGQIPP
                     DVSSEDVRNNPRYLSAILALKDSVETEQEGNSDIINITASSSDPKQAQRIANTVAQVY
                     REFHTRDLNKRIIESKKFIEDQVTTTRQKLEDSENAVRDFRQAYRMVSLDSQSSGLVA
                     QVTTLQTVYDRDTAILGRIRDIAAFLESAEDRPLSSKNSFYFEEASAPYKSLNERLVQ
                     LMLERDILLINYTENFPQVIEIKKKIHEIITSMKAQLRAQQQSLSHGIGVTQKQLRAY
                     DSQLKDLPTLGLELARLEREVAVNREIYTMLEKQHQEALIQDAEQLEEVQIVKPALEP
                     TAPINPPKTAASTGLGFIIGIILGLVFAFLIETFDTSIGAVEDVEEFLGVRVLGIIPF
                     IDFDELRQAFREKSGVEIDDDRMWRISRMISHFAPKTTSAEGYRALRTNINFAKLEKD
                     VKTIVFTSSSPQEGKTTISINLAIAMAQAGNKVLLIDGDFRRPVISKILGIPSQPGMT
                     DVILGNFEWREVVRSITDIMTGKMTMDEIMMTPGLDNLSVMTCGTIAPNPSELVSTKA
                     VSDFLEAANDEYDFVIVDAPPILAATDAAIWGTRVDGTIIVYQVGKIARGALKRAKAQ
                     LDNVKANIVGVVLNGLKAEISPDYEYHDKYYYYYGSERKKRLSLGERIASLPESLRKS
                     VQDLPGKVSQLRKARPSPQINLKSALKWVLLLIACVLLGTGIYFSGLLKGVLPVEPSA
                     PTQSSSAPAAAKPSSEAPAPATPDASKTRDVLPPSSNVDSGSKPVDTPVAPKSAVSTI
                     PPTKPLDKAPASIGPRYSIQVRATQDGRTATDLISLLKKQGHPVYAETADLKKRGVWR
                     RIFIGPFPSENDARRYVNTHKLSG"
BASE COUNT         1329 a         1817 c         1547 g         1221 t
ORIGIN      
        1 atctctttca ggtcgggacg cataaacggc tccccgccgg aaagcgtcac cgtcatgacg
       61 gcgcagcgac ccagttcatc aaaaaaacgc agccatgccg acagcgggag atccgtgtcc
      121 acatccccgg aactcgagaa atggctgcaa taggcgcagc gtaaattaca gaagctcgtg
      181 atctcgatgt ccacgctgcg cggcgatttc atgacgcttg gaaacatagg ggcctcaggc
      241 tgcttttggg ccggccaggc caaactcgac ggtcgcccgg acaaagtccc ggatatgttc
      301 agctacgtcc tctgggactt cttctgccgc ttcgcggatg atatccgtga tcttctcaag
      361 gctgtggcaa ccgtcgagaa gtttccagac catcacgcct atgggattga ttccgaaggc
      421 acttcctgtg tccgggtcga agaggatcgc ccagtcgtcg aattcctccc gcaggacaac
      481 gaggggattt gccatgatga gtgtttcaga tgctggttcc atcgcttatt cctacaagaa
      541 tcgcgggagg ttgtcaacgc gaatgcgtcc gaaaacagcg gtcggtgagg aaggaatatc
      601 ttactgatat atctcgtctt tcagaatgga accctggatg ttgaggatgt tgttatctgc
      661 caggtcaatc cgccggtctt ccagtaagcg ctttccgtga ccgtccataa caagattgat
      721 gacgggcttg agcgggttcg tcgggttggt tttaacgacg atgccgatgg ccttgttgtt
      781 cagcaggacg tagcttccta tggggtacat cgtgatctcg tccaggaaga tcttgatgat
      841 gtgcggagag aagagcgcgt ccttggcctg ggcgatctcg agaatggatt tgtgctgagc
      901 cccggccttg cgataggcgc ggtcgtgggt catggcttca tacgaatcgc agatcgcgat
      961 gatcttggca tactcgcaga tttcctcgcc cttgaggcca aggggatagc cccggccgtt
     1021 ttcccgctca tggtgttcat aaacggcgcg gctcacctgg ggataggttt cgtccaacgc
     1081 tttcaaaatg tcccttccat aggcggtgtg ctttttgagc tcggtaaatt cctcttcggt
     1141 caacctgccc tcttttcggg aaatggcatc gtcgacgaaa accatgccga tgtcgtgatg
     1201 aagcgccgcc atgcagacct cggtcagttt gaatgcggaa tagcccatcc tctttccgac
     1261 cttgaggctg tagatcatgc tgttgacggg cgaaacaata tcgagatcgt cattatggcc
     1321 gccctccgcg gtcaatttat aaagttcgtc gacaagtgga gcatcctcga tgatcgcttc
     1381 aatcatattc agggacagcg ccagattaac cggttccccg gcccggagtt tatccctgat
     1441 gtccagaagg taattttggg catcctgata gaggccctcc gggcgatagg atccgcccgc
     1501 ctcaaatgac tcaggctccg ttccatcgga agcagggctt tccgagaggg actcttgcgt
     1561 ctcagaggta tcggaggttt cttcaggggg ctgactttct ggcgcggagg ggaaaaggta
     1621 acggagattg tacttcaccc cctgctccgg cgtctctttt tttgccgttg ttgtcggcgt
     1681 attgcccctg atcagatctg aaatgcgcac catgtgaagt accggccccc cgctcattcc
     1741 gtcggatctc gcatgatccc gatattgttg gccttaaact atagggtcca acacattaaa
     1801 atgcaagcaa aatcttaggg tcgtcaaaat acacgtcccg tcaaaaatga cttgacattt
     1861 ttagatcttt tccacgatat tacacgcact cattcaacca ccttctttgg cgcgaggagt
     1921 tcccatgaac ttcaggataa tgcgagtgtt ctcgtccgtc attctcgctc tctgctccct
     1981 ggtcatcctc tcctgtaccc ttgaatatcc gggcattccc gtgaaaacct atcaggaacc
     2041 cgccaccgtc ctcgaggctc gagaccggga gcagcaggag atggcggaaa cgatccgggg
     2101 actgagcgac gtcacggaaa actccgtctt caccgaaaaa tatggaaccc ccgaatatcg
     2161 ggtcggctcc ggagacgtca tcacgattaa ctactgggtc ccctcccccg acgaaggctt
     2221 caagcaacaa acctatacgg caaccgtccg gccggacggc aagatctcct tcgtctacgc
     2281 ggacgacatt tttgtcgaag ggatgaccgc ccgcgagatc gacgacaccc tgacccggct
     2341 gtctcagaaa tacctacgcg atccccgaat cgaggtcatg gtcaaggaat tcaaaagcaa
     2401 gtctatcttg atcttcggcc gcatcaacat catggatcag caggcgggcg tctcgggtcc
     2461 gggacgctat ccgcttgtcg gcaagacgag ggtccttgac gtcatcatca cggccggcgg
     2521 tcctgtcgcc ggtcaggaca cgggaaacgc caacctgagg caggttgagc tcctgcgcca
     2581 gggtaagcga tattttctca atatttacaa cgcattcatc ggcggggatg ccatcgacaa
     2641 cgtggtcgtc gaacacggcg acgtcatcaa cgtcccggaa atgccgacct acgccgagcg
     2701 catctatgtc tttgggcagg tgcggtccca ggggatcctg cgcctgcggg attctaacga
     2761 tcttttgagc gccatcgcca tctccggcgg caccacgccg gtcgccgtca agaccgacat
     2821 caagatcatc cgggactacg atgcccagaa acgcggcaaa cccattatcc tttccgcgaa
     2881 tctggaccag atcctgatgc agggcgacct cgcccagaac atccccctgc acgacggaga
     2941 cgtcatctac gttccgcgca gcgtcatcgg ggacatcaac gaattcatcg agaacatcac
     3001 ccccatgctc gacctgatcc agaaccgtcc ggctgaattc cgtacgaatt acttcaagga
     3061 tcagaccaaa ctgcgctggt aagccaagga gcccgcccgc catgccgatg acctacgacc
     3121 tgaacctgcg cgagtactgg cgcacgatcc gcaggcgcaa gttcatcatc gtcttcaccg
     3181 tcgtcatgat gaccctcttc agctttttct tttcgatcat gggtcgtccg accccgatct
     3241 acaagacgag cgcgagcgtc aaggtcgaaa aatcaggctc ggtgaccggc ctgtacatcc
     3301 gggcggtttc ctggtccacc acggactaca tggaaaccca gatggcgatg atcaaaagct
     3361 atctgatcat ggagatggtc gccaagaagc tcggccaaat cccgcccgat gtctcctccg
     3421 aggacgtgcg caacaacccg cgttaccttt ccgccattct cgccctgaaa gattccgtcg
     3481 agacggaaca ggaaggcaac agcgacatta tcaatattac tgcaagctcc tcggatccga
     3541 aacaggcgca acgcatcgcg aacacggtcg cccaggttta cagggaattt cacacccgtg
     3601 acctgaacaa gcggatcatc gaatccaaga aattcatcga ggatcaggtc accaccacgc
     3661 gccagaagct cgaggattcc gaaaatgccg tgcgggattt ccgccaagcc taccgcatgg
     3721 tttcgctgga ttcgcaatcc agcggtcttg tcgcccaagt gaccacgctg cagaccgtct
     3781 acgaccgcga cacggccatc cttggacgga tccgggacat cgccgccttc ctcgaaagcg
     3841 ccgaggaccg gccgctcagt tcgaaaaaca gcttctattt cgaagaggcc tccgccccct
     3901 acaagagcct caacgaacgc ctcgttcagc tcatgctgga gcgcgacatc ctgctgatca
     3961 actatacgga aaacttcccg caggtgatcg aaatcaagaa gaagattcat gagatcatca
     4021 ccagcatgaa ggcccagctt cgggcccagc agcagagcct atcgcacggt atcggcgtga
     4081 cgcaaaagca gttgcgcgcc tacgacagcc agctcaagga cctcccgacc ctgggcctcg
     4141 aattggcgcg tctcgaacgg gaagtggcgg tcaaccgcga aatctacacc atgctggaaa
     4201 agcagcacca ggaagccctt atccaggatg cggagcaact cgaagaggtc cagatcgtca
     4261 agccggcgct cgagccgacc gccccgatca atccgccgaa aacggccgcc tcgacgggcc
     4321 tgggattcat catcggcatc atcctgggac tcgtctttgc cttcctgatc gaaacgttcg
     4381 acacctccat cggcgccgtg gaggacgtgg aggaattcct gggtgttcgc gtcctcggca
     4441 tcatcccctt catcgatttc gacgaactgc gccaggcctt cagggaaaaa tccggggtcg
     4501 aaatcgacga cgatcgcatg tggcggattt cgcgtatgat ctcgcacttc gctccgaaga
     4561 ccacttcggc ggaaggctat cgcgccctgc gcaccaacat caacttcgcg aagctcgaaa
     4621 aggacgtcaa gacgatcgtc ttcaccagtt cgtcgcccca ggagggaaag accaccatct
     4681 ccatcaacct ggccatcgcc atggcgcagg cgggcaacaa ggtgctgctg atcgacggag
     4741 acttccgccg tcccgtgatc tcgaagattc tcggcatccc ttctcagccc gggatgacgg
     4801 acgtcattct cggcaatttc gaatggcggg aggtcgtccg ttccatcacc gacatcatga
     4861 cgggcaagat gaccatggac gagatcatga tgaccccggg tctcgacaac ctttccgtca
     4921 tgacctgcgg caccatcgcc ccgaatccgt ccgaactcgt cagcaccaag gccgtctccg
     4981 acttccttga ggcggcaaac gacgaatacg attttgtcat tgtcgacgcc ccgccaatcc
     5041 tggcggcaac ggatgcggcg atctggggga cgcgcgtgga cggcaccatc atcgtctacc
     5101 aggtcggcaa gatcgcccgc ggtgctctga aacgcgccaa ggcccagctt gacaatgtga
     5161 aggccaacat cgtcggcgtg gtcctcaacg gtctcaaggc cgaaatcagc cccgactacg
     5221 aatatcacga caaatattac tactactacg gcagcgaacg gaaaaagcgc ctctcgctcg
     5281 gcgaacgaat cgcgtctttg ccggaatccc tcagaaaatc cgtccaagat ctccccggca
     5341 aggtcagtca actcagaaag gcccggccat ccccccaaat caatctcaag tccgccctga
     5401 aatgggtgct gctcttgatc gcctgcgtac tgctcggcac cggcatctat ttctcgggcc
     5461 tcttgaaggg cgttcttccc gtggaacctt cggcgcccac tcagagctcc tccgcaccgg
     5521 ccgccgcaaa accgtcaagc gaagcgccgg cgcctgctac gccggatgct tctaaaaccc
     5581 gggatgtcct ccctccctcc agcaacgttg attcgggatc caaacctgtc gatacccctg
     5641 tcgcgccgaa gagcgccgtt tcaaccattc cccctacgaa gcccttggac aaagctccgg
     5701 cgtcaatcgg cccacgttat tccatccagg tgcgcgccac ccaagacggg cgcacggcga
     5761 ccgacctgat ttctctcttg aagaaacagg gtcaccccgt ctatgccgaa acggctgacc
     5821 tcaaaaaaag aggggtttgg cgccgcatct tcatcgggcc cttcccttcc gaaaacgatg
     5881 cccgtcgata tgtcaacacc cacaagcttt ccgg
//
LOCUS       DAOOLJ010000089         5807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_16527_length_5807_cov_17.605007, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000089 DAOOLJ010000000
VERSION     DAOOLJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16527_length_5807_cov_17.605007"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="ligA"
                     /locus_tag="PLW16_09625"
     CDS             complement(<1..1331)
                     /gene="ligA"
                     /locus_tag="PLW16_09625"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPB71210.1"
                     /translation="MDPIRRRMAELAAEIAHHNRRYYQMDDPEISDATYDTLMRDLQT
                     LEAAHPDLAAADSPTRRVGAPPLEKFGAVRHRTPMLSLSNAFSESDIQTFGERLQRLL
                     GEEGPLLRFIVEPKLDGVAVGLTYTRGALTTGATRGDGLTGEDVTANIRTIPDVPLRL
                     NTGDDRPLPEFLEVRGEVYMERPAFEGMNRRRLLAGEPPFANPRNAAAGSLRQLDSRI
                     TARRPLRMYCYAVGAVSGLPPFRSHTDVLEALRGWGFSVNPEIRAVRDLPGCIAEYRR
                     IGAIRTSLPYEIDGIVIKVDNLGLQEHLGTVSRSPRWAIACKFDALQETTVVRDIVVQ
                     VGRTGVLTPVAVMAPVQVGGVTVSRATLHNEDEIRRKDIRIGDTVLIQRAGDVIPEVV
                     KVIESRRTGVERPFAMPATCPECGSDVVRLQGEVAHRCLGLACPAQLKEHIR"
     gene            complement(1337..1606)
                     /locus_tag="PLW16_09630"
     CDS             complement(1337..1606)
                     /locus_tag="PLW16_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71211.1"
                     /translation="MIEKAVNQLADKILNLDEASMTALWEKYKDRMERFEVSREWERS
                     VIVFFIINAVRAKNHIFNEQVAARQHPPADTPNRRRGKPELIRVK"
     gene            1922..2413
                     /locus_tag="PLW16_09635"
     CDS             1922..2413
                     /locus_tag="PLW16_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPB71212.1"
                     /translation="MKVMSAPWRMDYIKSKKKAGCVFCKDDVRGEELVLWEGETTYVM
                     MNRYPYISGHLMIIPYRHLSRVEDLSPDERREMFDLMDMSIRVLTDVMHPEGFNAGIN
                     LGKAAGAGIDDHLHIHVVPRWVGDTNFMSVVGEVRVIPEELRKTWEHLCPHFEKYCRE
                     GLR"
     gene            2410..2721
                     /locus_tag="PLW16_09640"
     CDS             2410..2721
                     /locus_tag="PLW16_09640"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPB71213.1"
                     /translation="MRVIYTIVVVLLVLFVITFSLENAANIPLKYYDFFNTNIPVYML
                     IFLAFLVGVVFTGFMGVIERFQLTRTINKLNKTVRDLRRELRDKEERPVVAEPRPPEI
                     R"
     gene            complement(2859..3350)
                     /locus_tag="PLW16_09645"
     CDS             complement(2859..3350)
                     /locus_tag="PLW16_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPB71214.1"
                     /translation="MDFRAAVVTLSDKGARGEREDLSGAECVRMLEEVGIPVADTRII
                     PDERLEIERTLIALAAQGLDLVVTTGGTGVSPRDVTPEATLAVIDRTLPGMAEAMRLA
                     SLAVTPHAMLSRAVAGLRNRTLIINLPGSPRGVRENLAVVLPALRHALEKIRGDESDC
                     ATP"
     gene            complement(3350..5428)
                     /gene="fusA"
                     /locus_tag="PLW16_09650"
     CDS             complement(3350..5428)
                     /gene="fusA"
                     /locus_tag="PLW16_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB71215.1"
                     /translation="MAKYEPKSLRNIAFVGHGGTGKTSLCESVLYVAGKTDRAGRVDD
                     GSSTMDYEPEEQRRHISIGAALNFVDWEKHKINLIDTPGDANFTYDTKNCLRIVDAAV
                     VLVDAVGGVEFQTEKVWTYADEFSLPRLVFINRMDRERADFPQALDSLKNRLRVKTTP
                     VCLPIGKEDQFKGIVDLIQMKALLFEGDKGAVKTADIPTDLREEAEKYRETMLEDIAE
                     SDEALMEKYLDVGTLTAEELTDGLRKGVCAGSVIPVMCGSALKHIGTAPLMSLIAQTF
                     PSPADRGPVTGLKPGSDEVETRSPEEAAPFSALVFKTIADPYAGRLTLFRVFSGAVSS
                     DASFHNATRKINERFGNLFFLEGKAQKPTDGVVAGDIAAVAKLKETVTGDTLCADKAP
                     VVFARVVPPRPVMSFAVEPKSRGDEDKLVSSINRLIEEDPTLTFHRDEQTKEMILSGM
                     GHIHIEVTTEKMKRKFGVEVNLKLPKVPYKETIKGKTNVQGKYKKQSGGRGQYGDCTL
                     DIEPLPRGGGFEFLDKIVGGVIPQQYRPAVEKGIVEAMEEGVVAGYPVVDVRVSLVFG
                     SYHTVDSSEMAFKIAGSMGFKKGVLACQPILLEPIVNVAIEIPDEYMGDVIGDLNSRR
                     GRVSGMDAEGSHQVIRGQVPLAEILKYAPDLTSMTSGRGTFTYDFSHYEEVPAFIAEK
                     IIAESKKEDA"
BASE COUNT          991 a         1737 c         1915 g         1164 t
ORIGIN      
        1 cggatgtgtt ccttcagctg ggcgggacag gccagcccga ggcagcggtg ggccacttcc
       61 ccctgcaggc ggacgacgtc cgacccgcac tccgggcagg ttgcaggcat ggcgaacggc
      121 cgctccacgc cggtccgccg gctctcgatg accttcacca cttcgggaat gacgtcgccg
      181 gcgcgctgga tgaggactgt gtcgccgatc cggatgtcct tacggcggat ctcgtcctcg
      241 ttgtgaaggg tcgcccggct gaccgtgacg cccccgacct ggaccggcgc catgaccgcg
      301 acgggcgtga gaacgcccgt acggccgacc tgaacgacga tgtcccggac gaccgttgtt
      361 tcctgtagcg cgtcgaattt gcaggcaatc gcccagcggg ggctgcggga gacggtaccg
      421 aggtgctcct gcaggccgag gttgtccacc ttgatgacga tgccgtcgat ttcgtagggg
      481 agggacgtcc ggatggcgcc gatgcggcgg tattccgcga tgcaccctgg aaggtcccgg
      541 acggcgcgga tttcgggatt tacggaaaat ccccaacccc ggagcgcctc cagaacgtcg
      601 gtgtgcgagc ggaagggggg gaggccgctg acggcaccga cggcgtagca gtacatccgg
      661 agcggccgcc gcgcggtgat ccgcgagtcc agttgacgca aggagccggc ggcggcgttg
      721 cgggggttgg cgaacggcgg ttcgcccgcg agtaggcgcc ggcggttcat cccctcgaag
      781 gccgggcgtt ccatgtatac ctcgccccgg acctccagaa attccgggag cggccggtcg
      841 tcgccggtgt tcaggcggag cgggacgtcc gggatggtcc ggatgttcgc ggtcacgtct
      901 tcaccggtca ggccgtcgcc ccgggtcgcg cccgtcgtca gggctccccg cgtatacgtg
      961 aggccgaccg cgacaccgtc gagcttcggt tccacaatga agcggaggag ggggccttct
     1021 tcgccgagca ggcgctgcag acgctccccg aaggtctgga tgtccgactc ggagaatgca
     1081 ttggacagac tcagcatcgg ggtccggtgg cggacggcgc cgaacttctc caggggcggc
     1141 gctccaacgc gccgggtagg ggaatccgcg gcggcgagat cgggatgcgc cgcctccagc
     1201 gtctggaggt cacgcatgag cgtgtcgtag gtggcgtcgg aaatctccgg atcgtccatc
     1261 tgataatagc gccggttgtg gtgggcgatc tccgccgcca gctccgccat gcgccttcgg
     1321 atggggtcca tgtccttcat tttacgcgga tgagttcggg tttcccccgc cgccggttgg
     1381 gcgtgtcggc cggcggatgc tgccgggccg cgacctgttc gttgaagatg tggtttttcg
     1441 cccgtacggc gttgatgatg aaaaaaacga tgacggaccg ctcccactct cgggagacct
     1501 cgaaccgctc catgcggtcc ttgtattttt cccagagagc ggtcatggag gcttcatcca
     1561 ggttgaggat tttatctgcg agttgattaa cggccttttc gatcatgttc ggtatccttc
     1621 cctcggaacg ccgcgcgtga gccgtcgatc ggtcggcccc ccggggcgtt ccgtgtgtgg
     1681 aggccaaatg cctttttgcc tgagatctta gccgccgggg cggggattgt caaatcaaaa
     1741 gccgctgcgt tgccctcccc ccgtaagtac tcgtggggtt ccggcttgag gattccttca
     1801 gaaataatgc ttgtcttttt ctccggtatc gctataatcc cacccgatcg ccctcgggaa
     1861 aggcagcgga attcgacggg aatgcccgtc gtggaaacaa tcgggcgaag gaccgtcggg
     1921 aatgaaggtc atgagcgccc cttggcggat ggactatatc aaaagcaaga aaaaggcggg
     1981 gtgcgttttc tgcaaggacg acgtccgcgg agaggaactg gtcctatggg agggtgagac
     2041 gacctacgtc atgatgaacc gctatcccta catcagcggt cacctgatga tcattcccta
     2101 ccggcatctc agccgggtgg aggatctttc tccggatgag cgccgcgaga tgttcgacct
     2161 gatggacatg tccatccgcg tgctgacgga cgtgatgcac ccggagggct tcaacgccgg
     2221 gatcaatctc gggaaggccg ccggggcggg gatcgacgac catcttcata tccacgtggt
     2281 cccccgctgg gtcggagata cgaacttcat gagcgtcgtc ggggaggtgc gggtgatccc
     2341 ggaagagctg cgcaaaacat gggagcattt gtgtccccat ttcgaaaagt actgtcggga
     2401 gggtttaaga tgagggtcat ctatacgatc gtcgtggttc tgctcgtcct gttcgtcata
     2461 acgttctcgc tcgagaatgc cgcgaacatc cccctgaagt actacgactt cttcaatacg
     2521 aacattcccg tctatatgct gatcttcctg gcctttttgg tgggggtcgt ctttacgggg
     2581 ttcatgggcg tcatcgaacg ctttcagctc acccggacga tcaataagct caacaagacg
     2641 gtccgcgatt tgaggcggga gctgcgggac aaggaagagc ggccggttgt cgccgagccc
     2701 cgaccgcccg agatccgtta ggggcttccg gatcggatga tatcggaggg ggatccgccg
     2761 gacgggcggt cccccctttt tttttgaccg gaattcgtcc gacggggcgc cggagatgcc
     2821 attgcctggg gaacgttcgg ctgaggccgg ggagaggttc agggggttgc gcagtcgctc
     2881 tcgtcgcccc ggatcttctc cagggcgtgg cggagggccg gcagaacgac ggcgaggttc
     2941 tcccggacgc cccgggggct gccgggcagg ttgatgatca gggtccggtt gcgcaggccc
     3001 gccacggcac gggagagcat ggcatgcggg gtgacggcca gactggccag gcgcatggct
     3061 tcggccatgc cgggaagcgt ccggtcgatg acggccaggg ttgcctcggg ggtgacgtcg
     3121 cgggggctca ccccggtgcc gcccgtggtg acgacgagat cgagcccttg ggcggcaagg
     3181 gcgatcaggg ttcgttcgat ttcaagccgt tcgtcgggga tgatccgggt gtcggcgacg
     3241 gggatgccga cttcttccag catccggacg cactccgccc cgctcagatc ttcccgctcc
     3301 ccgcgcgcgc ccttgtcgct cagggtgacg accgcggccc ggaaatccat caggcatctt
     3361 ccttcttgga ctcggcgatg atcttctccg cgatgaacgc cggcacctcc tcatagtggg
     3421 agaaatcgta cgtgaaggtc ccgcggccgc ttgtcatgga ggtcaggtcc ggggcgtatt
     3481 tcaggatttc ggccaggggc acctgtcccc ggattacctg gtgggacccc tccgcatcca
     3541 tgccggaaac ccgtccccgc cggctgttca gatcgccgat gacgtcgccc atgtactcgt
     3601 cggggatctc gatggcgacg ttcacgatgg gttcgaggag aatcggctgg caggccagaa
     3661 cccccttctt gaagcccatc gatccggcga tcttgaaggc catttccgag gaatcgacgg
     3721 tatggtagct cccgaagacg agggaaacgc ggacgtccac gaccgggtag cccgcgacga
     3781 cgccttcctc catggcctcg acgatgccct tttcgacggc cggccgatac tgctggggga
     3841 tgacgccgcc gacgatcttg tcgaggaatt cgaatccacc gccccggggc aggggttcga
     3901 tgtccagcgt gcagtcgccg tactgcccgc gaccgccgga ctgcttcttg tacttgccct
     3961 ggacgttggt ctttcccttg atggtttcct tgtaggggac tttggggagc ttcaggttca
     4021 cctcgacgcc gaatttccgc ttcatcttct ccgtggtgac ctcgatgtgg atatgtccca
     4081 tgccggagag aatcatctcc ttcgtctgct catcccggtg gaaggtcagg gtcggatcct
     4141 cttcgatgag ccggttgatg gacgagacga gcttgtcctc gtccccgcga gatttgggct
     4201 cgacggcaaa ggacattacc ggtctcggcg ggaccacccg cgcgaagaca acgggcgcct
     4261 tgtcggcgca caacgtgtct ccggtcacgg tttccttgag cttcgccacg gcggcgatgt
     4321 cgcccgcgac gacgccgtcg gtgggcttct gagccttacc ctcgaggaag aagaggttgc
     4381 cgaaacgttc gttgattttg cgagtggcgt tatggaagct cgcatcggag ctgacggcgc
     4441 cggaaaagac ccggaacagc gtgagccggc ccgcgtaggg gtcggcgatc gtcttgaaga
     4501 ccagggcgga aaacggcgcc gcctcctcgg gcgagcgcgt ctcgacctca tcgctccccg
     4561 gcttgaggcc cgtcacggga ccgcggtccg cgggggaggg gaaggtctgc gcgatcaggc
     4621 tcatcagagg cgcggtgccg atgtgcttca acgccgatcc gcacatcacc gggattacgc
     4681 tgccggcgca gacgcccttg cgaaggccgt ccgtcagttc ctcggcggtc agcgtgccga
     4741 cgtccaggta tttctccatc agggcctcgt cggattcggc gatgtcctcg agcatcgttt
     4801 cccggtattt ctctgcctct tcccgcaggt ccgtgggaat gtcggccgtc ttgacggctc
     4861 ccttgtctcc ctcgaagagc agggctttca tctgtatgag gtccacgatt cctttgaact
     4921 gatcttcctt gccgatggga aggcacacgg gcgtggtttt cacccgcagg cgattcttga
     4981 ggctgtccag ggcctggggg aaatccgccc gctcgcggtc catgcgattg atgaacacca
     5041 ggcggggcag agagaattcg tcggcgtacg tccagacctt ctccgtctgg aattcgacgc
     5101 cgcctaccgc gtccacgaga acgacggcgg cgtccacgat ccggaggcag ttcttggtgt
     5161 cataggtgaa gttggcgtcc cccggggtgt cgatcaggtt gatcttgtgc ttttcccagt
     5221 ccacgaaatt cagggcggcc ccgatggaga tgtgccgtcg ctgctcttca ggctcgtaat
     5281 ccatcgtgga agatccgtcg tccacacggc cggcgcggtc cgttttgccc gcgacgtaaa
     5341 gaacggactc gcacagggac gtcttgcccg tcccgccgtg tccgacaaag gcgatgtttc
     5401 tcagggattt cggttcgtac ttggccatga agactccttt cacgtcgtgc cctgccgttt
     5461 ttggcgggct gaaccccggc ggacgggaaa tggttttata ggccggctcc gatccgcgcg
     5521 tggacggtgg taaattaacg gtgctgctta gatcataccc tccgaaaaag tcaagtcaat
     5581 tttggcgcaa gcgcctggcc ggcgccaaat atccattgac aggaaaatga gatcccagta
     5641 taagttccga cccctaaaac agggtcaggg ggcgtccgct acggcggatc ggccggcagg
     5701 gttggaaacg ttacacgatg gggaagtctc gaacaatgaa agaaaagtcc atcagaaaaa
     5761 tcctcattgc aaaccggggg gaaatcgccc tgcggatcct gaggacg
//
LOCUS       DAOOLJ010000090         5724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_16854_length_5724_cov_19.375375, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000090 DAOOLJ010000000
VERSION     DAOOLJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16854_length_5724_cov_19.375375"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLW16_09655"
     CDS             <1..777
                     /locus_tag="PLW16_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB71216.1"
                     /translation="QVPVINFALGKGDWLVREAHKYGGKVIATVVNARHAKRAQDYGS
                     DGVIATGNEAAAHGEAVTSLVLIPSLADAVEIPVIAAGGFADGRGLAAALALGAEGIA
                     MGTRFMTTKDSPLHGNFKKLSIEKDVTDTLYSTRIDGIYCRVLKTEPAAKQIKKGLDL
                     FSAFFNSQDISKQLHMPYLKLMIGILASGWKNTRQMAYMANGFKAFRLATEEGDLVRG
                     ILPVGQVTGLIHDEPSVAEVMDRIVAEAVEVQAKLAAKMA"
     gene            complement(909..1958)
                     /locus_tag="PLW16_09660"
     CDS             complement(909..1958)
                     /locus_tag="PLW16_09660"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71217.1"
                     /translation="MKYRVIQWATGSMGKTCLRAVIDHPDLELVGLYVYSDRKIGRDA
                     GEIARRAPTGVIATNRIEDILALEADVVIHAPRIQFPYRHHNANMCRLLASGKNVITI
                     NGHGFPAYHGPAYAAEFERACAQGNASLFSTGLNPGFVVEKIATAATGICLSLDAIQV
                     QEVFDISGAPDHDYVFNVCGFGSDPDRFDLTGKGPVAELITGMYAEVLALLVNRLGMT
                     LDRVEPDHRVLPAPADIQGRAGVVTAGTVAATCWRWHAVVDGRRLVTLSVNWVMGNTL
                     PGFETYDHWKIAIRGKPGIDIAMNIVEPENTGEKTRGEQYAVAGSVVNAIPEVCAAPA
                     GIFRFPLFAPYRPRF"
     gene            complement(2141..2794)
                     /gene="rpe"
                     /locus_tag="PLW16_09665"
     CDS             complement(2141..2794)
                     /gene="rpe"
                     /locus_tag="PLW16_09665"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB71218.1"
                     /translation="MKKIAPSILSADGSRLAEEIRAVEAAGADWIHVDVMDGHFVPNL
                     TLGPSMVAAIRKVTTLPLDVHLMIEAPEGFIPEFARAGSDWITVHVEAAHHLHRTVGQ
                     IREAGCRAGVSLNPATPLVQVEEILPDLDLLLVMSVNPGFGGQRFIDGALPRIRRAKE
                     MIAAAAPRVLLEVDGGVTVDNIGAIAQAGADVLVAGAAIFGSPDYARTIAAMKTRAA"
     gene            2958..4286
                     /locus_tag="PLW16_09670"
     CDS             2958..4286
                     /locus_tag="PLW16_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPB71219.1"
                     /translation="MLYHLYNLVLVLAAVFAVPYYGAKILFQGKYRRSIGPKFGLIRP
                     DLFARMEGTPRIWMHAVSVGEVTAAAPIIASLREHFPAACIVLSTSTETGQEMARRIA
                     TAATVSIYYPLDLGCVVRRVIDRVRPDLFIPVETELWPNFLRHCKDRGVRVVMVNGRI
                     SPRSFRRFVLTRFFWREVLAAVDEMGVISQADAERVEAMGVPAKKIHVLGNAKYDSLA
                     AKASPKIREDIARRLGLTEPEPVFVAGSTHEGEEAIVLDVYRRLLEVVPAAQLILIPR
                     HVERAAAVLTLVREAGFPDCITMSELNKGKKRAGERVIVIDVIGELFKVYSLATAVFC
                     GGSLVQKGGQNILEAAAWGKVVFYGRFMDDFREERALLEETGAGITVRDGADLAARLI
                     EMIRHPEERRRLGEKGRDRVIANMGAAGRYAAMIRMQVKKTGTAGPKTKG"
     gene            complement(4404..5306)
                     /locus_tag="PLW16_09675"
     CDS             complement(4404..5306)
                     /locus_tag="PLW16_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB71220.1"
                     /translation="MPENLFKVNKEEYMLPGNRACQGCGLSLGYRYILKALRENTILT
                     IPASCLTVLHGLYPTTSVALPCLNCTFASTAASASGLVAGLNALNRGDITVVAVAGDG
                     GTYDIGIQALSGAAERGTDFIFVCYDNEGYMNTGTQRSSATPMGALTTTTPIHTKQQN
                     KKDMLKIMEAHDLPYIATVSPAYPMDLYDKFVKAKRIKGTRYLQMSAPCPPGWGFPPK
                     DMVKLSRLAIETGMFVLYEVENGRFSFTGRSKTLAEKGKRLPVINYLERQGRFRKMSL
                     EQLSQIQRWVDGKWNEYVRRAETN"
     gene            complement(5311..>5724)
                     /locus_tag="PLW16_09680"
     CDS             complement(5311..>5724)
                     /locus_tag="PLW16_09680"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPB71221.1"
                     /translation="DADVILTGMGSIATEATMAVDALREQGIAAGVVGLRVYRPFPKA
                     AVREAFRDARAVLVMEKDISYGYEGALCSDLKAAFYGTEISAPIHNVVAGLGGRDVKA
                     RELAEVTREILDRIAAGQPEGPTTWLNCRTDTLTE"
BASE COUNT          963 a         1801 c         1966 g          994 t
ORIGIN      
        1 caggtgccgg tcatcaactt tgccctgggc aagggcgact ggctcgtccg ggaggcccac
       61 aagtacggcg gcaaggtcat cgccaccgtc gtcaacgccc gccacgcgaa acgcgcgcag
      121 gattacggca gcgacggcgt catcgccacg ggcaacgagg cggcggcgca cggcgaagcg
      181 gtgacctcgc tcgtgctgat tcccagtctg gccgatgcgg tcgagatccc ggtcatcgcc
      241 gcgggcggat tcgcggatgg gcgcgggctc gccgccgcac tcgccctggg cgccgagggc
      301 atcgccatgg ggacgcgctt catgacgacg aaggacagcc ccctgcacgg caacttcaaa
      361 aagctctcga tcgaaaagga cgtcaccgat acgctctatt ccacgcgcat cgacgggatc
      421 tactgccgcg tcctgaaaac ggagccggcg gcgaagcaga tcaaaaaggg gctcgatctg
      481 ttttcagcct tcttcaattc ccaggatatc tcgaagcagc tccacatgcc gtacctcaag
      541 ctcatgatcg ggatcctggc ctcgggctgg aagaacacgc gccagatggc ctacatggcc
      601 aacggtttca aggcctttcg tctggcgacg gaggagggcg acctcgtccg cgggatcctg
      661 ccggtggggc aggtcacggg actgatccac gacgagccct cggtggccga ggtcatggac
      721 cgtatcgtgg cggaggccgt ggaggttcag gcgaaactcg ccgccaagat ggcgtaggcg
      781 gggacgcaac ggaggatatc gggaaacgca aagagaagcg gcggaaaagg gatctgcatc
      841 gaccggtgcg gatccttttt ttgtgtgcgc ggcgtcgttc acgacgccgc gccggacgtg
      901 gcgcgtccct aaaagcgggg gcggtagggg gcgaacaggg ggaagcggaa gatgccggcc
      961 ggggcggcgc agacctccgg aatggcgttg acgaccgaac cggcgacggc gtactgctcc
     1021 ccccgggtct tctcgccggt attttcgggc tcgacgatat tcatggcgat atcgatgccg
     1081 ggtttgccgc gaatggcgat cttccagtga tcgtacgttt cgaagcccgg tagcgtgttg
     1141 cccatgaccc agttgaccga aagcgtcacg agccgccggc cgtccacgac ggcgtgccag
     1201 cgccagcagg tcgccgcgac ggtgccggcg gtgacgacgc cggccctccc ctgaatgtcc
     1261 gccggcgccg ggaggacacg gtggtcgggt tccacgcgat cgagggtcat ccccaggcgg
     1321 ttcacgagca gggcgagcac ttcggcgtac atccccgtga tgagctccgc caccggcccc
     1381 tttcccgtca gatcgaaccg gtccggatcg gaaccgaacc cgcagacgtt gaacacatag
     1441 tcatggtccg gcgcgccgct gatgtcgaaa acctcctgca cctggatggc gtccagcgaa
     1501 agacagatcc cggtggccgc agtggcgatc ttctcgacga cgaacccggg attgaggccg
     1561 gtgctgaaaa gggacgcgtt cccctgggcg caggcccgct cgaactcggc ggcataggcg
     1621 gggccgtgat aggcggggaa cccatgcccg ttgatcgtaa tgacgttctt ccccgaggcc
     1681 agcaggcggc acatgttcgc gttgtggtgg cgatagggaa actggatgcg cggggcgtgg
     1741 atgaccacgt cggcctccag ggccaggatg tcctcgatcc ggttcgtcgc gatgacgccc
     1801 gtcggcgccc gccgggcgat ctcacccgcg tcacggccga tcttccggtc gctgtagacg
     1861 taaagcccca cgagttccag atcgggatgg tcgatcacgg ccctcaggca cgttttcccc
     1921 atggagcccg tcgcccattg aatcacgcgg tatttcatcg gccgtttccc ttccaaccca
     1981 ggatgtgttc acaaaaggtc aaggatcgct ttttgaggtc cgcgaaattg ggcgtccctg
     2041 tctatgactt ttggcttccc gcttacgcgg ggacggcagg ggcgcgggcc tggatgcggg
     2101 ggtgacggga acgggtgccg gcctcaacgc gatgtcccga tcaggcggcg cgggttttca
     2161 tggcggcgat cgtccgggca tagtcggggc tgccgaagat cgccgccccc gcgacgagga
     2221 cgtccgcccc ggcctgggcg atcgccccga tgttgtccac cgtgacgccg ccgtccacct
     2281 ccaggaggac ccggggtgcg gcggccgcga tcatctcttt ggcccggcgg atcctaggca
     2341 gggcgccgtc gatgaaccgc tggcctccga agccggggtt gaccgacatg acgaggagca
     2401 ggtcgagatc cgggaggatt tcctccacct gaacgagggg cgtcgcggga ttgagcgata
     2461 cgccggcgcg gcaacccgcc tcgcggatct gccccaccgt ccggtgcaga tggtgcgccg
     2521 cctccacgtg gaccgtgatc cagtcgctgc ccgcccgggc gaactccggg atgaaaccct
     2581 cgggtgcttc gatcatcagg tggacgtcga ggggcagggt cgtgaccttc cggatggcgg
     2641 cgaccatgga cggtcccagg gtgaggttcg gaacgaagtg gccatccatg acgtccacgt
     2701 ggatccagtc ggcccccgcc gcctccacgg cgcggatctc ttccgccagg cggctaccgt
     2761 ccgcggacag gatggaaggg gcgatctttt tcatgcctgc tccatacagg attatcccgt
     2821 cgctgtcaac cggcctccct gcgggccgcc cgcggccccg cctccggccc gggcgggaat
     2881 gtccttgaca gacggggcca aaggggcgat gatccccgcg atttgaaccg agggagggct
     2941 tcccggggag cataaagatg ctgtaccatc tgtacaatct ggtcctggtc ctcgcggcgg
     3001 tcttcgccgt gccctactac ggggcaaaaa tcctcttcca gggaaagtac cgccggagca
     3061 tcggcccgaa attcggtctg atccgtccgg acctgttcgc ccggatggag gggacgccgc
     3121 ggatctggat gcatgccgtc tccgtcgggg aggtcacggc ggcggcgccg atcatcgcct
     3181 cgctccggga gcatttcccc gcggcctgca ttgtcctgtc cacgagcacg gaaacgggcc
     3241 aggagatggc ccgcaggatc gccaccgccg cgacggtttc gatctactac cccctcgatc
     3301 tcggatgcgt ggtgcgacgc gtcatcgacc gtgtccgtcc cgatctgttc atccccgtcg
     3361 aaacggagct ttggccgaat tttctccggc actgcaagga ccggggcgtc cgcgtggtca
     3421 tggtgaacgg ccggatttcg ccccgctcct tcaggcgctt cgtcctgacg cgctttttct
     3481 ggcgggaggt cctggcggcg gtggatgaaa tgggggtcat ttcgcaggcc gatgccgaac
     3541 gggtggaggc catgggggtg cccgcgaaga agatccatgt gctgggcaac gccaagtacg
     3601 acagcctggc cgccaaggcg tcgccgaaga tccgcgagga catcgcgcgg cgcctggggc
     3661 tgacggagcc ggaaccggtc ttcgtcgcgg ggagcacgca cgaaggggag gaggcgatcg
     3721 tgctcgacgt ctatcgccgc ctgctcgagg tggttcccgc cgcccaactg atcctgatcc
     3781 cccgtcatgt cgaacgggcg gcggcggtgc tcacactcgt ccgggaggcg ggattcccgg
     3841 actgcatcac gatgtcggaa ttgaacaagg gcaaaaagag ggcgggggag cgcgtcatcg
     3901 tgatcgacgt gatcggcgag ctcttcaagg tgtacagcct ggcgacggcg gtcttctgcg
     3961 gggggtcgct cgtccagaag ggcgggcaga acatcctcga ggcggcggcg tgggggaagg
     4021 tcgtgttcta cgggcgtttc atggacgact tccgggaaga gcgggcgctg ctggaggaga
     4081 ccggggcggg catcacggtg cgcgacggcg cggacctcgc cgcccgtctg atcgagatga
     4141 tccgccaccc ggaagagcgc cgccgcctgg gagagaaggg ccgggacagg gtcatcgcga
     4201 acatgggcgc cgccggccgt tacgccgcga tgatccggat gcaggtcaaa aagacgggca
     4261 ccgccgggcc gaagaccaaa ggctaaaggc caagagctaa aaagtgaaaa gttgaaggca
     4321 aaatctaagg gctgaaagct gaaggctgaa gggttcccct gcgcgaatgt ccgtttatga
     4381 atcctccgct ctgacgctag ccttcagttt gtttccgccc gcctgacgta ctcgttccac
     4441 ttgccgtcca cccagcgctg gatctggctt aactgctcga ggctcatctt ccggaaacgg
     4501 ccctggcgct ccaggtagtt gatcaccggc agccgctttc ccttctccgc cagggtcttg
     4561 ctccgtccgg tgaagctgaa ccgcccgttc tcgacctcgt agaggacgaa catcccggtt
     4621 tcgatggcga gccgggagag cttgaccatg tccttgggcg gaaagcccca gcccgggggg
     4681 cagggggccg acatctggag gtagcgcgtg cccttgatcc gcttggcctt gacgaacttg
     4741 tcgtagaggt ccatgggata cgccggtgag acggtcgcga tgtagggcag atcgtgtgcc
     4801 tccatgatct tcagcatgtc ctttttgttc tgctgtttgg tgtggatcgg cgtggtcgtc
     4861 gtaagcgccc ccatcggggt cgcgctggag cgctgcgtcc ccgtgttcat gtagccttcg
     4921 ttgtcgtagc agacgaagat gaagtccgtt ccccgctccg cggccccgct cagggcctgg
     4981 atgccgatgt cgtaggtccc gccgtcgccc gcgacggcca cgacggtgat gtcgccccgg
     5041 ttcagggcgt tgagtccggc caccagcccg gacgccgagg ccgccgtgct cgcgaacgtg
     5101 cagttcaggc agggcagggc caccgaggtc gtggggtaaa gcccgtgcag gaccgtgagg
     5161 caactggccg ggatggtcag gatcgtgttt tcccggaggg ccttcaggat gtaccggtag
     5221 cccagggaca ggccgcaacc ctggcaggcc cggtttccgg gcagcatgta ttcttccttg
     5281 ttcaccttga agagattttc cggcatggtt tcactccgtg agggtgtcgg tgcgacagtt
     5341 gagccaggtg gtcgggcctt ccggctgacc ggcggcgatg cggtccagga tctcccgcgt
     5401 gacctcggcg agctcacggg ccttgacgtc ccgtccgccc aggccggcca cgacgttgtg
     5461 gatgggggcg gagatctccg tcccgtaaaa ggccgccttg agatccgaac agagggcgcc
     5521 ttcatacccg tagctgatgt ccttctccat cacgaggacg gcgcgggcgt cccggaaggc
     5581 ctcccgcacg gccgccttgg ggaaggggcg gtagacgcgg aggccaacga cgccggccgc
     5641 gatgccctgc tcccggagcg cgtcgacggc catcgtggcc tcggtggcga tcgagcccat
     5701 gccggtgagg atcacgtcgg cgtc
//
LOCUS       DAOOLJ010000091         5663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_17109_length_5663_cov_16.449893, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000091 DAOOLJ010000000
VERSION     DAOOLJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17109_length_5663_cov_16.449893"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="PLW16_09685"
     CDS             complement(<1..1561)
                     /locus_tag="PLW16_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPB71222.1"
                     /translation="MRRFKRLLVPFDATSNWNIRYGIQIAHAFHAHVHFIHVRPTYAR
                     IFPFRTLDGSGAAPSETLTEEERRRIRDRMLRHLRQFPLTGVSHTLTVLHGLPAREIL
                     NAIGETKPDLVILGTEGMMKFEDILMGLSSLKIIENSPVPILSIRPDLSPPFGSSQPL
                     DSEPPAQGLYAIRRILAPTDFSAASREAVVYAGAIAHYLRAELHLLFSAADRDEAEST
                     PASGAHENLQRLAQELTALYGELPFVPQVSNLDSVKAILDTTRRIRMDLIVMSVHGEQ
                     GARFPIGSTTRRIIQRARCPVLTLHPRMKSSRIETRYQRVFKTLGLADLTQSHDGALR
                     PFGPGELSPGGARSDLFLKHYSPEGLRTVLEAYGIFPLLRKKGFSSLQIKLHMTDPYR
                     QRVRVIFDGQEDENHLIAEVVMREAALSAPAGGGTASHRPFSVLMIEWLYLQNPLVSF
                     SPDRPPLPGQSYPGLRISHEILELLIFAGRRLGKDAVTIRPAHFHSAKMYHQQFSCLD
                     PRREGQLIALIR"
     gene            complement(1567..2970)
                     /locus_tag="PLW16_09690"
     CDS             complement(1567..2970)
                     /locus_tag="PLW16_09690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPB71223.1"
                     /translation="MTAKRLHTRRRGLWPILFLTLLVSASLPSAASGAFTLADERKLG
                     KEFYDKLNQNGVIVQDERIALYVRNLGADILAHSHPAPFDFTFSVIDSSAVNAFATPG
                     GYIYVNRGLVNLVKTESELAGVLAHEIAHVNRRHIAELIARSKKISIASLAAILASAF
                     LGGGGELSAAAVGFSMAAATHLNLKYSREHEEEADRLGMAYLAGAGYNPQSMLDFLKS
                     MRRYEFYSSNIPSYFLTHPGTDERIRYLDGLLLTTYRHEGREDRHGQLKRVKSTLAAR
                     GRVSDSLLRQYREDVREHPRDVDALFGLALLLDRSGQTNEALSCFRQSLALAPNDQEI
                     RLQMGRTLLKAGQIESSIALLRTAYEQNPADASVLESLAEAYDTRGSFSEALSLYREL
                     SRLQPANVDVLHRMAMNCGRLGQHGESHLLFGRYFKNKNRVDSAEYHYRQALRYYPPE
                     SDRGREIQSELESLKTL"
     gene            3213..3917
                     /gene="cmk"
                     /locus_tag="PLW16_09695"
     CDS             3213..3917
                     /gene="cmk"
                     /locus_tag="PLW16_09695"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPB71224.1"
                     /translation="MIVTIDGPAGSGKSTIAKAVARRLAFAYLDTGALYRAVAVEIRR
                     RHLSTENVKGLSALFSETTLSLVRDGTEQRVTVNDRDVTDELRSEAVSLLASRISAKP
                     EVRAFLLDIQRRFGRSGRLVAEGRDMGTVVFPEAEIKFFLDARPEERARRRYRELRQK
                     GEAVDYDEILADLIVRDRQDRERLHAPLRAAPDAVVIDTTRLRIDEAVDAILGVIRAH
                     LEGDRILPGPWSPFTA"
     gene            4002..>5663
                     /locus_tag="PLW16_09700"
     CDS             4002..>5663
                     /locus_tag="PLW16_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPB71225.1"
                     /translation="MVNDNNEMPNREEESVIDTGEESSQATRDEEDLGFRELYEQSLQ
                     SVQIGEVVTGKVVQIGSDAVMVDVGWKTEGYIPLRELIDAEGKIGISVGDELEILVDR
                     RDGDGNLVLSRDKATKIKVWDDIKEACEQNLPVQGTIMERIKGGLSVDIGIPAFLPGS
                     QVDIRPVRDLDRYVGQNLMFEVLKYDKKRNNVVLSRRTILEKEREEEKKRTLETIEEG
                     KIVQGIIKNITDYGLFIDLGGIDGLLHVTDISWGRMSRPADKFSRGDSITVKVLSFDR
                     EKERVSLGLKQLIDNPWDQIQSRYPVGAVFEGKVVNLTDYGVFVELEDGVEGLVHISE
                     MFWTREIKHPTKVLSLGQVINVMILDVNPVSKRISLGWKQTTPNPWESLKQKYPEGQI
                     IKGVIRNITNFGIFVGVEDGIDGLVHVSDISWKQRVKHPSEFYKKGQEIEAVILNIDV
                     ENEKFSLGIKQLEKNPWEELGERFPAGSTVSGRVTNMTDFGIFVEIEEGVEGLVHISE
                     LSSKRIKTPSELYSVGDTVSAVVKNIDQKGKKIRLSIKDFESGTTD"
BASE COUNT         1170 a         1507 c         1883 g         1103 t
ORIGIN      
        1 cgcggatgag cgcgatcaat tgtccctccc ggcgcggatc cagacaggaa aactgttgat
       61 ggtacatttt cgcgctgtgg aagtgagcgg gacgaatggt gacggcgtcc ttgcccagtc
      121 ggcgtcccgc gaaaatgagc agctcgagga tctcatggct gatgcgcagg ccggggtagc
      181 tctggccggg aaggggaggg cgatcgggtg aaaaggagac cagcggattt tgcaggtaaa
      241 gccattcgat catcaggacg ctgaagggtc tgtgtgacgc cgttccgccg ccggcgggcg
      301 ccgacagtgc cgcctcgcgc atgacgacct ctgcgatgag gtgattttca tcctcctgtc
      361 cgtcgaaaat gacccgcacg cgctggcggt aaggatccgt catatgcaat ttgatctgca
      421 gcgaggagaa tcctttcttt ctcaggagtg gaaagatccc atacgcttcc agaacggtcc
      481 ggaggccctc cggggaatag tgcttgagaa agagatccga ccgggcccct ccgggcgaca
      541 gctccccggg tccgaagggg cgaagggcgc catcgtgact ctgggtgaga tcggcgagcc
      601 cgagggtctt gaacacccgc tgataccgcg tttctatccg ggatgatttc attcttggat
      661 gcagtgtcag gaccggacag cgcgcccgct ggatgattct ccgggtggtg ctgccgatgg
      721 ggaagcgcgc gccttgttcg ccatggacgg acatgacgat caggtccatt cggatgcgcc
      781 gggtcgtgtc taggatggcc ttcaccgagt ctaagttcga aacctgcggc acgaacggca
      841 gttcgccgta gagggcggtc agctcctgcg cgagacgctg aagattttcg tgcgcgccgg
      901 atgctggcgt cgattccgcc tcgtcacggt ccgcggcgga gaacaggagg tgcagttccg
      961 cccggagata gtgggcaatg gcgccggcgt agacgacggc ctcccgggaa gcggccgaga
     1021 agtcggtcgg agcgagaatc cggcggatgg catagaggcc ttgggcgggt ggctcggagt
     1081 cgagcggctg ggaggacccg aagggcggcg agaggtccgg acggatcgag aggataggaa
     1141 ccggagagtt ttcgatgatt ttcagggagg acagtcccat gaggatgtcc tcgaatttca
     1201 tcatgccctc ggtccccagg atcacgaggt ccggtttcgt ttctccgatg gcgttcagga
     1261 tctcacgggc aggcaagccg tggaggacgg tcagggtatg ggatacgccg gtcagcggga
     1321 attgccgcaa gtggcgaagc atccgatccc ggattcgccg tcgttcctct tccgtcaggg
     1381 tttcggacgg tgcggccccg gatccgtcca gggtgcgaaa tgggaagatg cgtgcatacg
     1441 tcggccggac atggatgaaa tggacgtgag catggaaggc gtgggcgatc tgaatcccgt
     1501 accggatgtt ccagtttgat gtggcgtcga aggggacgag aagccgcttg aatctgcgca
     1561 tcgcgttcag agagttttca gggattcgag ttcgctctgg atttcgcggc cgcggtcgct
     1621 ttccggcgga tagtatcgca gcgcctgccg gtagtggtat tccgcgctgt ccaccctgtt
     1681 tttgtttttg aagtagcgcc cgaagagcag atgcgattcg ccgtgctgtc ccaggcggcc
     1741 gcagttcatc gccatccggt ggaggacatc gacgttggcg ggctgaagcc tagagagctc
     1801 acggtagagc gaaagtgcct cggaaaacga tccccgggtg tcgtaggcct cggcgagaga
     1861 ttccagaacc gaggcgtcgg ccgggttctg ttcgtacgcg gttctcaaca gtgcgatcga
     1921 ggattcgatc tgtccggcct tcaaaagcgt gcggcccatt tggaggcgga tttcctgatc
     1981 gttgggggcg agggccagcg actgacggaa gcaggagagc gcctcgttcg tctggccgga
     2041 gcggtccagc aggagggcca ggccgaacag ggcgtccacg tcacgggggt gctcccgaac
     2101 gtcctcgcgg tattggcgga gcagggagtc ggaaactctg ccgcgggcgg ccagggtgga
     2161 tttcacgcgc ttgagctgtc cgtggcggtc ctcccggccc tcgtggcgat aggtcgtcag
     2221 cagcaggccg tccagatagc gaatccgttc atccgtcccg gggtgagtta gaaaatagga
     2281 ggggatgttg ctggagtaaa attcgtagcg gcgcatggat ttgaggaaat cgagcataga
     2341 ctggggattg tagcccgctc cggccagata ggccatgccc agacggtcgg cctcctcttc
     2401 atgctcacgg ctgtatttga ggttcaggtg ggtcgccgcg gccatcgaga aaccgacggc
     2461 ggcggcggaa agttcccccc cgcccccgag aaaggccgag gccaggatgg ccgccagcga
     2521 ggcgatgctg attttttttg atctcgcgat gagttccgcg atgtggcgcc ggttgacgtg
     2581 cgcgatttca tgggccagga cgcccgccag ctcactttcc gtcttgacga ggttgaccag
     2641 tccccggttg acgtagatat atccccccgg tgtcgcaaag gcgttgacgg cggagctgtc
     2701 gatgaccgag aacgtgaagt cgaagggggc aggatgactg tgcgcgagga tgtccgcccc
     2761 caggttgcgg acgtagagag cgatgcgttc atcctgaacg atcacgccgt tttggttgag
     2821 cttgtcatag aactccttgc cgagtttcct ttcgtccgcc agggtaaagg ccccggaggc
     2881 cgcgctgggt aaggaagcgg agaccaggag ggtgagaaaa aggatgggcc aaaggcctct
     2941 tctccgcgtg tggagacgct ttgcagtcat gattccattg ccctcttcgg tcgtacgggt
     3001 taaaaagggt tatgaccaag cttaaccctt ttacgggaaa atggcaagag cgctccgggt
     3061 gcggcccaat gcacggttgt catgaatgct gcgattgtgc tagacaggca ggcaaacgcc
     3121 catcggtgcg catgggggtg tatccggttt ccgagagcgc ttccggcaag cgtccgggtc
     3181 gcgaaagcca ttcctgcagg cagaaaaaag ccatgatcgt caccatcgat ggcccggctg
     3241 gttcgggtaa gagcacgatc gccaaagcgg ttgcacggcg cctcgccttc gcctacctcg
     3301 acacgggcgc cctgtatcgt gccgtggccg tcgagatccg gcgtcggcat ctgtcgaccg
     3361 agaacgtcaa aggcctgtcc gccctttttt cggaaaccac cctctccctg gtccgggacg
     3421 ggacggagca gcgggtgacg gtcaacgaca gggatgtgac cgacgaattg cggtcggaag
     3481 ccgtcagcct gctcgcgtcg cggatatcgg cgaaaccgga agtccgggcc tttctcctcg
     3541 acatccaacg ccgcttcgga cggtccggta gactggtcgc cgagggacgc gacatgggca
     3601 cggttgtctt tccggaggcg gaaatcaagt tctttctcga cgcccgaccc gaggaacggg
     3661 cacggcgccg ttatcgagaa ctgcggcaga aaggtgaggc ggtcgattac gacgaaattc
     3721 ttgcggacct aatcgttcgg gaccggcagg accgggagcg gttgcatgcc ccgctcagag
     3781 cggctccgga cgcggtggtc attgatacca ctcggctgcg catcgacgag gccgtggatg
     3841 cgattctggg cgttatccgg gctcatctgg agggagatag aatcctgcca ggaccttggt
     3901 cccctttcac cgcctaattt cttgctattt gggtggcagt atgctagttc tccaaaacta
     3961 tcggccgttt attcaacaaa catcgggggg gagagggttt aatggttaac gacaacaacg
     4021 aaatgccgaa cagagaagaa gaaagcgtca tcgatacggg ggaggaatcc tcccaggcga
     4081 cccgggacga ggaagatctg ggttttcgcg agctatacga gcagagcttg caaagcgtcc
     4141 agatcggcga agtcgtgacc ggcaaggtcg tccaaatcgg cagcgatgcc gtgatggtgg
     4201 atgtgggctg gaaaacggag gggtatatcc cgctccggga gctcatcgat gcggaaggga
     4261 aaatcggcat ctccgttggt gacgagttgg aaattctggt ggaccggcga gacggggatg
     4321 gaaatcttgt cctgtcacgc gacaaggcca ccaagatcaa ggtctgggac gacatcaagg
     4381 aggcgtgcga gcagaacctg cccgtgcagg ggaccatcat ggaacggatc aagggggggc
     4441 tctccgtcga tatcggcatt cccgccttcc tgcccggttc gcaggtcgat atccgacccg
     4501 ttcgggatct ggatcgttat gtcggccaga acctgatgtt cgaggttctg aaatacgaca
     4561 agaagcgcaa caacgtcgtt ctgtcccggc gcaccattct tgagaaagag cgggaggaag
     4621 aaaaaaagcg caccctcgag acgatcgagg agggcaagat cgttcagggg atcatcaaga
     4681 atataacgga ctacggcctg tttatcgacc tcggcggaat cgacggactg ctgcatgtca
     4741 cggacatttc ctggggccgc atgtcgagac ccgcggacaa gttttcacgg ggggacagca
     4801 tcacggtgaa ggtgctgtcc ttcgatcgcg agaaggagcg cgtttcgctc ggcctgaaac
     4861 aactgatcga caatccgtgg gatcagatcc agagccgcta tccggtcggt gccgtcttcg
     4921 aggggaaggt cgtcaacctg acggattacg gcgtcttcgt cgaactcgaa gacggcgtcg
     4981 agggcctggt ccatatctcg gagatgttct ggacacgaga gatcaagcac ccgaccaagg
     5041 tcctgtccct gggacaggtt atcaatgtca tgattctgga cgtcaacccc gtctcaaaac
     5101 gcatttcgct gggatggaag cagacgactc cgaatccctg ggaatccctg aagcagaaat
     5161 acccggaagg gcagatcatc aagggcgtca tccgcaacat cacgaatttc ggaatctttg
     5221 tgggtgttga ggatggcatc gacggcctgg tccatgtttc cgacatctcg tggaaacagc
     5281 gggtcaagca cccgagtgaa ttttacaaga aaggccagga gatcgaggcc gtcatcctga
     5341 atatcgatgt ggaaaacgag aagttctcgc tcgggatcaa gcaactggag aagaatccct
     5401 gggaggagct tggcgagaga tttcccgccg gatcgaccgt cagcggtcgc gtcaccaaca
     5461 tgacggactt cggaatcttc gtggagatcg aagagggcgt ggagggactc gttcacattt
     5521 cggagctcag ttcgaagcgc atcaagaccc cgtccgagct ttacagcgtg ggcgacacgg
     5581 tttccgccgt ggtgaagaat atcgatcaga agggcaaaaa gatccgcctc agcatcaagg
     5641 atttcgagtc cggaacgacc gat
//
LOCUS       DAOOLJ010000092         5499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_17786_length_5499_cov_21.705547, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000092 DAOOLJ010000000
VERSION     DAOOLJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17786_length_5499_cov_21.705547"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLW16_09705"
     CDS             <1..1044
                     /locus_tag="PLW16_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB71226.1"
                     /translation="LEALESSILDAIPHAVIGLHNREFIFANNAVRSVFGWRPEELIG
                     KGVRLLYRGNEDSEAIAGKFYATLETQRTFAMEFPCRRKDGEDILCMVRASRIGESLR
                     ERRIVVTYEDITERRRAEEELERSREELRNLSSHLQSVREQERTQIAREIHDELGQSL
                     TALKMDLTWMGKRLQDNEPLLDKTRSMGKLIDGTIEMVHRISADLRPGLLDDFGLVPA
                     LEWFAKDFQKRSGIVCRARLEVDDLALDKEMSTTIFRIFQETLTNVARHAEARRVEVR
                     LAASGGRLILEVSDDGRGITKKQMNDPRSFGIIGMRERAHSWGGEVLISGNRDKGTTV
                     RLTIPLAAGEAGA"
     gene            1041..1712
                     /locus_tag="PLW16_09710"
     CDS             1041..1712
                     /locus_tag="PLW16_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB71227.1"
                     /translation="MIRILVVDDHTIVREGLKQILGEEKDMNVVEEASNGQEALQKIG
                     KSEYDCVLLDISMPGRSGLEVLKDIKALRPKLPVLILSMHSEELYAIRALRAGASGYL
                     TKASASEELIGAIRKVAAGRKYVSTSLAEKLAVELDADASKPPHETLSDREYQVMIML
                     ASGKTVKEIADEVCLSVKTISTYRTRIMEKMNLKKNAELTLYAIQHHLIAYDEQANLN
                     DSIFF"
     gene            1880..4054
                     /locus_tag="PLW16_09715"
     CDS             1880..4054
                     /locus_tag="PLW16_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPB71228.1"
                     /translation="MRTSEMEAKTGLQEWTSQPVGQARPRRAKRREPASEFRDILIQA
                     HRENRSYLMEHECKELLEGMGIATTGYLVAGSEDEAVEMANAVGYPVVLKIVSPDVVH
                     KTDAGGVKLNLKHADDVRRGYQAIVGAFKYQHIVGVSVQKMAAPGVEAIIGVTRDPSF
                     GPVLMFGLGGVFVEVLKDVTFRVLPVTEADIAEMIGEIKGYPLLKGHRGVSVDIPALK
                     ALLMKISALVMAFPEIGELDLNPVFLYPEGNIVVDARIFVRDGEDAAPALAAKKQNMR
                     DFFYPKSIAVLGASGVPGKLGYNVFRNLIFHEFKGRIYPINAKNDYVQGVKAYKNILD
                     VPDPVDLAIVLVPAGAAQAAVEDCCKKGVKFLVVETAGFAETGEAGKKVQARIKEIIE
                     ANGCRVLGPNCSGVINTHHNMVQSIGLIEELGKGNVGMVAQAGVYAAGIITGLRHVMD
                     FGIVATIGNKMDISEADLLEYLGQDDHIDVIAMYMEDMKSGRRFIDVASEVTKKKPLI
                     VLKNGRTEAGKKAVSSHTASLAGNDTINTAAFRQSGAIRARDNEHMFGLMKAFSKQPL
                     PKGDGILVITYTGSLGVAATDVLYLNGMRLGDLEPHLKTRLMELLPDYVKTLNPVDYS
                     FSMDPEQLVKTIEIGVESEDIGGFIVVIQGEILDRFVEPLRAVNTQGKPILSCVACKE
                     FMMDDVVKMEKAGIPVYSTVEMATEVMADMYRFWLRKANQEQ"
     gene            complement(4227..5153)
                     /locus_tag="PLW16_09720"
     CDS             complement(4227..5153)
                     /locus_tag="PLW16_09720"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPB71229.1"
                     /translation="MIIVNTLQNVSPDLKGAVITIGNFDGVHIGHRRIFEKVIADARH
                     LGGPAVVMTFEPHPKQVLHPERRPFYLITTPAEKLRLIEAAGVDAVWLVPFSLDFAAR
                     TATSFVEDILIGALAVRKVTIGHDYTFGRGREGNEAFLKAMGRKRGFEVDVVNAFRIG
                     ETIISSTAIREAILAGEVSRAAAMLGRPYNLAGRVVVGHRRGAGLGFPTANLEPEKVL
                     VPARGVYAVRARVSGGSYAGVLNIGENPTFGDGRRSVEVHLLGFTGDIYGEPLEVQFI
                     ERLRGEVKFDGPDALVAQIRKDIDRARRILSV"
     gene            5339..5425
                     /locus_tag="PLW16_09725"
     tRNA            5339..5425
                     /locus_tag="PLW16_09725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5373..5375,aa:Leu,seq:cag)
BASE COUNT         1057 a         1781 c         1798 g          863 t
ORIGIN      
        1 ctggaggccc tcgagtcctc catcctggac gccatccccc atgccgtgat cggcctgcac
       61 aaccgggagt tcatcttcgc caacaacgcc gtccggtccg tgttcgggtg gcgcccggag
      121 gagctcatcg gcaagggcgt gcgcctgttg taccgcggca acgaggactc cgaggcgatc
      181 gccggaaagt tctacgcgac cctggagacc cagcggacgt tcgcgatgga attcccctgc
      241 cgccggaagg acggggagga catcctctgc atggtccggg cctcgcgcat cggcgagagc
      301 ctgcgggagc gccggatcgt cgtcacgtac gaggacatca cggagcgccg acgggcggag
      361 gaggaactgg aacgctcccg ggaggagctc cggaacctct ccagccacct gcagtcggtc
      421 cgggagcagg agcgcaccca gatcgcgcgg gagatccacg acgaactggg ccagtcgctc
      481 acggccctca agatggacct cacctggatg ggcaagcgcc tccaggacaa cgaaccgctc
      541 ctggacaaga cgcggtcgat ggggaagctc atcgacggca ccatcgaaat ggtgcaccgg
      601 atctccgcgg acctgcggcc gggcctgctc gacgacttcg gcctcgtccc ggccctggag
      661 tggttcgcca aggacttcca gaagcgctcc gggatcgtct gccgggcgcg cctcgaggtt
      721 gacgatctcg ccctggacaa ggaaatgtcc acgacgatct tccgcatctt ccaggaaacc
      781 ctgaccaacg tggcccgcca cgccgaggcc cgccgcgtgg aggtccgcct cgccgcctcc
      841 ggcgggcggc tgatcctcga ggtcagcgac gacgggcggg gcatcacgaa gaagcagatg
      901 aacgaccccc gctccttcgg gatcatcggg atgcgggagc gggcccactc ctggggtggg
      961 gaggtgctca tttcgggcaa ccgcgacaag gggacgaccg ttcggctgac gattccgctg
     1021 gcggccgggg aggcgggggc atgatccgga tcctcgtcgt cgatgaccac acgatcgtcc
     1081 gggagggcct gaagcagatc ctcggcgagg agaaggacat gaacgtcgtc gaggaggcga
     1141 gcaacggcca ggaggccctc cagaagatcg ggaaaagcga atacgactgc gtcctgctcg
     1201 acatctccat gcccggccgg agcgggctgg aggtgctcaa ggacatcaag gccctgcgcc
     1261 caaagctccc ggtgctcatc ctcagcatgc attccgagga gctctacgcc atccgcgccc
     1321 tgcgcgccgg cgcctccggc tacctgacga aggcgagcgc ctcggaggag ttgatcggcg
     1381 ccatccggaa ggtcgccgcc ggacggaagt acgtcagcac gtcgctcgcg gaaaagctcg
     1441 ccgtcgagct ggacgcggac gcgagcaagc cgccgcacga gaccctgtcg gaccgcgaat
     1501 accaggtgat gatcatgctg gcctcgggca agacggtcaa ggagatcgcc gacgaggtgt
     1561 gcctgtccgt caagacgatc agcacctacc ggacgcggat catggagaag atgaacctga
     1621 aaaagaacgc cgaactgacc ctctacgcca tccagcacca cctgatcgcc tatgacgagc
     1681 aggccaacct gaacgactcc atctttttct aaaacctttc ttcccccgct tgtcggacaa
     1741 agaccgacac gacaatcatc ccgaatccga cgcgagaaat ggcgcccggc cgatagcggg
     1801 accccgtcct ccatgctaaa aatgcaccca aaagacacac ccgcggggcc cgcggggtca
     1861 gtaaaggggg acgaaagcca tgaggacgag cgaaatggag gcgaaaacgg gacttcagga
     1921 atggacgtcg cagccggtcg gccaggcgcg gccccgccgc gccaaacggc gggagccggc
     1981 gtcggagttc agggacatcc tcatccaggc gcaccgggag aaccgctcct acctcatgga
     2041 gcatgagtgc aaggagctgc tcgaaggcat ggggatcgcc acgacgggct acctcgtggc
     2101 cggctccgag gacgaggcgg tggagatggc caacgccgtc ggctaccccg tcgtcctcaa
     2161 gatcgtctcc cccgacgtcg tgcacaagac cgacgcgggc ggcgtgaagc tcaacctcaa
     2221 gcacgccgat gacgtccgcc ggggctatca ggcgatcgtc ggcgccttca agtatcagca
     2281 catcgtcggc gtctccgtcc agaagatggc cgcccccggc gtcgaggcca tcatcggcgt
     2341 cacccgcgac cccagcttcg gcccggtcct gatgttcggt ctgggcggcg tgttcgtgga
     2401 ggtcctgaag gacgtgacct tccgggtcct gcccgtcacc gaggccgaca tcgccgagat
     2461 gatcggggag atcaagggtt accccctgct caaggggcac cggggcgtgt ccgtcgacat
     2521 ccccgcgctc aaggctctgc tcatgaaaat ctccgcgctc gtcatggcct tcccggagat
     2581 cggcgaactc gacctgaacc cggtgttcct ctaccccgag ggcaacatcg tcgtggacgc
     2641 ccggatcttc gtccgggacg gggaagacgc cgcaccggcc cttgcggcga agaaacagaa
     2701 catgcgcgac ttcttctacc cgaagagcat cgccgtgctc ggcgcctccg gcgtgccggg
     2761 caaactcggt tacaacgtct tccgcaacct gatcttccat gaattcaagg gcaggatcta
     2821 ccccatcaac gcgaagaacg actacgtcca aggggtgaag gcctacaaga acatcctgga
     2881 cgtgcccgat cccgtggatc tggccatcgt cctggttccg gcgggcgccg cccaggccgc
     2941 ggtggaggac tgctgcaaga agggcgttaa gttcctcgtc gtggagacgg cgggtttcgc
     3001 cgagacgggc gaggcgggca agaaggttca ggcccgcatc aaggagatca tcgaggcgaa
     3061 cggctgccgg gtgctcggcc ccaactgctc cggcgtcatc aacacccacc acaacatggt
     3121 gcagtccatc ggcctcatcg aggagttggg caagggcaac gtcggcatgg tggcccaggc
     3181 cggcgtgtac gccgccggga tcatcaccgg gctccggcac gtcatggatt tcgggatcgt
     3241 ggccaccatc ggcaacaaga tggacatcag cgaggccgac ctgctcgaat acctggggca
     3301 ggacgaccac atcgacgtca tcgccatgta catggaggac atgaagagcg gccgccgctt
     3361 catcgacgtg gcgagcgagg tgacgaagaa gaagcccctc atcgtcctga agaacggccg
     3421 gaccgaggcg ggcaagaagg ccgtctcctc gcacacggcg tcgctcgccg gcaacgacac
     3481 gatcaacacg gcggccttcc ggcagtccgg cgccatccgc gcccgcgaca acgagcacat
     3541 gttcggcctc atgaaggcct tcagcaagca gcccctgccg aagggcgacg ggatcctcgt
     3601 catcacctac accggctccc tgggcgtggc cgcgacggac gtcctgtacc tgaacgggat
     3661 gcgcctgggc gatctcgaac cccacctgaa gacgcgcctg atggagctcc tgcccgacta
     3721 cgtcaagacg ctgaaccccg tggactactc cttcagcatg gatccggaac agctcgtcaa
     3781 gacgatcgag atcggcgtcg agagcgagga catcggcggc ttcatcgtcg tcatccaggg
     3841 cgaaatcctg gatcggttcg tcgagccgct cagggccgtc aacacccagg gcaagccgat
     3901 cctgtcctgc gtcgcctgca aggaattcat gatggacgac gtggtcaaga tggaaaaggc
     3961 gggcatcccc gtctactcca cggtcgagat ggcgacggag gtcatggcgg acatgtaccg
     4021 gttctggcta aggaaagcga atcaggagca ataatcccag cagtcttctc cccctcttcc
     4081 ggcgggcgcg ggcctttcga agtgaacgcc gggggaggac aaaataaagg ccgctgtcgc
     4141 cctcggggcg acagcggcct tttcattaaa aggtccagcg tgcgggggaa gccggttttg
     4201 aagcggtgtg caggtttagt ccggcctcag acggacagga ttcggcgggc ccggtcgatg
     4261 tcctttcgaa tctgggcgac gagcgcatcc ggaccgtcga acttgacctc cccccgcagg
     4321 cgctcgataa actgcacctc gaggggttcg ccgtaaatgt cgccggtgaa gccgaggaga
     4381 tggacctcga cggaccggcg cccgtcgccg aaggtgggat tctcgccgat gttgaggacg
     4441 cccgcgtagg acccgccgga gacgcgggcc cggacggcgt agacgccccg ggcgggcacg
     4501 aggaccttct ccggttcgag gttggccgtg gggaagccga gtccggcgcc gcgccggtgc
     4561 cccaccacca cgcggcccgc gaggttgtag ggccggccga gcatggcggc ggcccgggaa
     4621 acctctccgg ccaggatcgc ctcccggatc gccgtgctgc tgatgatcgt ctccccgatc
     4681 ctgaaggcgt tgacgacgtc gacctcaaac ccccgtttcc gccccatcgc cttcaagaac
     4741 gcctcgttcc cctcccgtcc ccgtccgaag gtatagtcgt gcccgatcgt caccttgcgg
     4801 accgcgagcg ccccgatcag gatgtcctcg acgaacgagg tggccgtccg ggccgcgaaa
     4861 tccaggctga aagggaccag ccacacggcg tccacgccgg ccgcctcgat gaggcgaagc
     4921 ttctccgcgg gcgtcgtgat caggtagaag ggtcggcgct ccgggtggag gacctgcttg
     4981 gggtggggct cgaacgtcat cacgacggcg gggccgccca agtgccgggc gtcggcgatg
     5041 accttctcga aaatccgccg gtggccgatg tgaaccccgt cgaagttgcc gatggtgatg
     5101 acggcgccct tcaggtccgg ggaaacgttc tggagggtgt tgacgattat catggggggc
     5161 ttcgtatcct tccgctgcgt tttgcggggg taacatatgc actgcacgcc ggaatatcaa
     5221 ggcaaatctg acgcgcccgc gaaacgtcgt cgtcgccgtc cggggccgtt gaccccaatt
     5281 ttgcttgaca agtttcgccc gctcagtgta gggaatagcc gctcttgatc gagaacgcgc
     5341 cgaagtggcg gaattggtag acgcgctagg ttcagggtct agtgggcgta cgcctgtccg
     5401 ggttcaaatc ccggcttcgg caccatgtgg gtaggaatgg gcgcaacttc atcgggttgc
     5461 gccttttcta ttttggcggg gtgcgcagag ggtgcgctt
//
LOCUS       DAOOLJ010000093         5414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_18170_length_5414_cov_17.049263, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000093 DAOOLJ010000000
VERSION     DAOOLJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18170_length_5414_cov_17.049263"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..477)
                     /locus_tag="PLW16_09730"
     tRNA            complement(402..477)
                     /locus_tag="PLW16_09730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Cys,seq:gca)
     gene            complement(487..561)
                     /locus_tag="PLW16_09735"
     tRNA            complement(487..561)
                     /locus_tag="PLW16_09735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(527..529),aa:Gly,seq:gcc)
     gene            complement(643..1497)
                     /locus_tag="PLW16_09740"
     CDS             complement(643..1497)
                     /locus_tag="PLW16_09740"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB71230.1"
                     /translation="MRIAQQPTSEMTHMIDPSTIHYPALDRPEITAFLFYPRPECETG
                     GAGRAEGIHMIPVEDGVSIGARFHLVDPAAPNLLFFHGNGEIVSDYDDIAGLYARIAI
                     NFLPVDYRGYGRSGGRPTVTAMMRDAHIIFASVRQWLADNRHTGPLIVMGRSLGSASA
                     LELASHYPDSVDGLIIESGFAHALPLLRLLGVDTDKLGITEGEAFDNTSKIARYPGPT
                     LVIHAEFDHIIPFADGEALFAASPDPDKRFIEIPEANHNDILLHGIQPYLDAVRRLAS
                     RCKQKSVL"
     gene            complement(1494..2111)
                     /gene="pgsA"
                     /locus_tag="PLW16_09745"
     CDS             complement(1494..2111)
                     /gene="pgsA"
                     /locus_tag="PLW16_09745"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPB71231.1"
                     /translation="MIEFLLKAFDRLPISPSRREVINLPNTITLIRIGFIPALFLLLL
                     APGPRASLILTAFFILVSFTDLLDGYIARKYKIVTKMGKFLDPIADKLVVNTAMLLMI
                     PIGRIPAWIVAITIIRDIAVDGMRSMASAEGLVIDASRLGKQKTLCQIFAVSALMIHY
                     PLFGINAHVVGMAVLYVALVLTVVSGADYFVKFYRGAIRERKDEA"
     gene            complement(2133..2780)
                     /gene="fsa"
                     /locus_tag="PLW16_09750"
     CDS             complement(2133..2780)
                     /gene="fsa"
                     /locus_tag="PLW16_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPB71232.1"
                     /translation="MKFFIDTANIDEINQGLAIGMVDGVTTNPTLIAREKKPFAWVVK
                     GILKAVEGPVSLEVIGTTAKEMVTEGRKLAKMGSNVVIKVPMTTEGLKATRLFSTEGI
                     RVNQTLVFSPVQALMAAKAGAAYVSPFVGRLDDIAHDGMELVDQILTIYENYGFETEV
                     IVASIRHPRHVLDAALMGADVATIPYKVIAQLAGHPLTDRGVVAFLEDWKKVPTK"
     gene            complement(2800..3069)
                     /locus_tag="PLW16_09755"
     CDS             complement(2800..3069)
                     /locus_tag="PLW16_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71233.1"
                     /translation="MFNLLRLIVIILILYFLYQGVRRFFLSDGKKMPIPGEDRAKQVR
                     GEDLVEDPQCHMYVPVSRAIEKRIGDRTLYFCSEACYRAYQESTK"
     gene            complement(3071..3595)
                     /gene="folK"
                     /locus_tag="PLW16_09760"
     CDS             complement(3071..3595)
                     /gene="folK"
                     /locus_tag="PLW16_09760"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPB71234.1"
                     /translation="MRSGIISFIGVGANLGRPEEQCRDALRRVGERDGIALLRVASLY
                     RTEPLGDPDQPCFVNTVAELRTTLSPLGLLSKLAAIEGEMGRRRSGRSAEPRLIDLDL
                     LLYGQEVVAEADLVVPHPRMHERRFVLVPMNEIAAYVLHPAFGVSMQGLLSRCRDSGR
                     VEKIGEGETHERTE"
     gene            complement(3611..4426)
                     /gene="dapB"
                     /locus_tag="PLW16_09765"
     CDS             complement(3611..4426)
                     /gene="dapB"
                     /locus_tag="PLW16_09765"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPB71235.1"
                     /translation="MGVKAIVTAAGGRMGGRIISLIDQTDGIELSGAVEVKGHPLVGR
                     KAGEVQGVGRPGVIVHDDLSACIDKGDVIIDFTHHAASLAHVKIAAQHGRAIVIGTTG
                     FSAVELQEIRKLAKDVRCVLAPNMSVGVNILFKVLHEVARILGDEYDVEIVEAHHNQK
                     KDAPSGTAMKMAQVIAEALNRDLGKAAVYHREGMIGARTQKEIGIQTLRAGDIVGEHT
                     VLFGGAGERLEFTHRAHSRDNFARGAIRAAKWVVGQPNGLYDMQDVLGLRERK"
     gene            complement(4515..5390)
                     /gene="dapA"
                     /locus_tag="PLW16_09770"
     CDS             complement(4515..5390)
                     /gene="dapA"
                     /locus_tag="PLW16_09770"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPB71236.1"
                     /translation="MFQGAIVAIVTPFHNGKVDEERLRELIEFQIAGGTDGIVPCGTT
                     GESATLSHEEHDRVIEITIDAVKKRVPVIAGTGSNSTREALRLTRHAHECGADGALMV
                     CPYYNRPGPEGLYQHFKAIAEAVPIPIIPYNIPGRTGVNMLPETVARLAKIPNIVGVK
                     EASGSLKQMSDIVRLCGERFSVLSGDDFFTLPLMAIGGKGVISVASNVAPADMAALVD
                     AAASGDMAQARSLHHKLTPLIDALFVETNPVPVKAALKLMGRIDDEVRLPLSRLSDAN
                     LDKLRGVMKAYGLIS"
BASE COUNT         1040 a         1705 c         1596 g         1073 t
ORIGIN      
        1 gtttgttttc cccttcatag actagcgcca cccacgcgtc ggcttcgatc gccacgatct
       61 tccccgttct caccgtagac atcgctccct cacccctttc cttcatctcg cactgcacct
      121 ccccacgacg ttcggatcca cccccgtcgt ccgcgctcct accccggcga cgtcgaaaat
      181 gcaacgagaa gccggttccg aaacggagcg atcatgacaa taggacggaa cttcgaaacc
      241 tttgcacaac agggaatttt gtcactacgg ccgaagggag aaatctttaa gaaacggcca
      301 ccatgaagat tcctcgtctc tcgcgctcct cgaaaggaca tgatgcaaag gattcacttc
      361 agagttgtgt gagcgccagg gttgcagaga caaaatagaa atggaggcgg catccggatt
      421 cgaaccggag aataacggtt ttgcagaccg ctgccttagc cacttggcta tgccgccata
      481 aaaaaatgga gcgggcgaac ggatttgaac cgtcgacgtc caccttggca aggtggcact
      541 ctaccgctga gttacgcccg ctcatcgaag acgttggctc attatagcaa ttgaccgttt
      601 ttgtcaacac aaaacttgaa cacccggagc gccccgcgcc tttcagagga ccgatttttg
      661 cttgcaccgc gacgcgagcc ggcgtacggc gtcgagatag ggttggatcc catggagaag
      721 gatgtcgttg tggttggcct cggggatctc gataaaccgt ttgtccggat ccggcgaggc
      781 cgcgaagagg gcctccccgt cggcaaaggg gatgatgtgg tcgaattcgg cgtggatcac
      841 tagggtcggc cctggatagc gggcgatttt cgaggtgttg tcgaacgcct ctccttcggt
      901 gatcccaagc ttgtcggtat cgactcccag gagtcggagc agcgggaggg catgggcaaa
      961 accgctttcg atgatcaggc cgtcgacgct atcgggatag tgggaggcaa gctccagggc
     1021 ggaggcgctc cccagcgacc gtcccatgac gatcagtggt cccgtgtgtc ggttatccgc
     1081 cagccactgg cgaacggatg cgaagatgat atgcgcgtct cgcatcatgg cggtcaccgt
     1141 cggccgaccg ccggagcggc cgtaaccgcg gtagtccacc ggcaggaagt tgattgcgat
     1201 ccgggcgtac agcccggcga tatcgtcata gtccgaaacg atctcgccgt tgccatggaa
     1261 aaaaaggagg ttgggcgccg ccggatcgac caggtgaaac cgggcgccga tcgagacccc
     1321 gtcttcaacg gggatcatgt ggatcccctc cgcccggccg gcccctcccg tctcgcactc
     1381 cgggcgggga tagaatagga aggccgtgat ttccggccga tcaagtgcgg gataatggat
     1441 ggtggacgga tcgatcatgt gggtcatctc cgaagtcggt tgctgtgcta tcctcacgcc
     1501 tcatccttcc gctcacggat cgcgcctcgg tagaatttga cgaagtaatc cgcgcctgaa
     1561 acaacggtca ggacaagcgc cacgtagagc accgccatgc cgacgacgtg ggcattgatc
     1621 ccgaagagcg gatagtggat catcagggcc gatacggcaa agatctggca gagggttttc
     1681 tgcttgccca gccggctggc atcgatgacc agcccctccg cggaggccat cgaacgcatg
     1741 ccgtccacgg caatgtcgcg gatgatcgtg atggcgacga tccacgcggg gatgcgtccg
     1801 atcggaatca tgagtagcat cgccgtattg acgacgagct tgtccgcgat cggatccaga
     1861 aacttcccca ttttcgtgac gattttatat ttgcgggcaa tgtacccatc gagcagatcc
     1921 gtgaacgaca ccaggatgaa gaatgccgtc aggatcaggc tcgctcgggg ccccggcgcg
     1981 agcaggagca ggaacagggc cgggatgaaa ccgatacgga tcaaggtgat cgtattgggg
     2041 agattgatga cctcccggcg cgacggggaa atgggaagcc gatcgaaggc cttgagcaga
     2101 aattcgatca tcgggcgcag cccttctttc ttctactttg tggggacctt cttccagtct
     2161 tccaggaagg ccacgacacc cctgtccgtc agcgggtgcc cggccaactg cgcaatcacc
     2221 ttgtagggga tagtcgccac atccgcgccc atgagcgccg catcgaggac atgccgggga
     2281 tggcggatac tcgcgacaat cacctcggtc tcgaagccgt aattctcgta aatcgtgagg
     2341 atctgatcga cgagttccat gccgtcatgg gcgatgtcgt cgaggcggcc gacaaacggg
     2401 ctgacgtagg ccgccccggc cttggccgcc atcagggcct ggaccggaga aaagaccaga
     2461 gtctggttga cgcggatgcc ctcggtcgag aagagccgcg tggccttcaa tccctccgtc
     2521 gtcatgggca ccttgatcac tacgttggac cccatcttcg ccagcttccg gccctcggtg
     2581 accatttcct tggccgtcgt accgatgact tcgaggctca ccggcccctc gacggccttg
     2641 agaatcccct tgacgaccca tgcgaagggt ttcttttccc gggcaatcag ggtcggattg
     2701 gtcgtcacac cgtccaccat gccgatggcg agaccctggt tgatctcgtc gatgttcgcg
     2761 gtatcgataa aaaacttcat acgtcctccc gatgcgtttt cattttgtcg attcctggta
     2821 ggcccggtag cacgcctcgc tgcagaaata cagggtgcgg tccccgatgc gtttctcgat
     2881 cgcgcggctg acgggtacgt acatgtgaca ctgtggatcc tcgaccagat cttcaccgcg
     2941 gacctgtttg gcccggtctt cgcccgggat cggcatcttc ttaccgtcgg agagaaaaaa
     3001 ccgtcgaacc ccctggtaaa gaaaatagag gatgagaatg atgacgatca atctcaggag
     3061 attaaacatt ttattcagtc ctctcgtgtg tctccccttc cccaatcttt tccacccggc
     3121 ccgaatcgcg gcatcgcgac agcaggccct gcatggaaac gccgaacgcg gggtgcagga
     3181 cgtaggcggc aatttcgttc atgggaacga gcacgaaacg ccgctcgtgc attctcgggt
     3241 gcgggacgac cagatcggct tcggcgacga cctcctggcc gtagagcagt aaatccagat
     3301 cgatcaaccg gggctccgcg gaccgcccgg agcggcgccg tcccatctcc ccctcgatcg
     3361 ccgccagctt tgacagcagc ccgagcggcg acaatgtcgt gcgcaattcg gcgaccgtgt
     3421 tgacgaagca cggctgatcc ggatcgccga gcggctcggt gcggtaaagc gaggccaccc
     3481 gaagcagggc gatgccgtcc cgttccccca cgcgccggag agcatcccgg cactgttcct
     3541 ccggcctccc gagattcgcg cccaccccga tgaagctgat gattcccgaa cgcatgctcg
     3601 gcctgacgtc ctatttcctt tcacgcaggc cgaggacatc ctgcatgtca tacagtccgt
     3661 tgggttgtcc gacgacccac ttcgcggccc ggatggcgcc gcgcgcgaaa ttgtcccgac
     3721 tgtgggcccg gtgggtgaat tccagtctct cccccgcgcc gccgaaaaga acggtatgtt
     3781 cgccgacgat gtcgccagcg cgaagggtct gaatgccgat ttccttctgg gtccgcgccc
     3841 cgatcatgcc ctcgcggtga tagacggccg cttttcccag gtcgcggttc agggcctcgg
     3901 cgatgacctg cgccattttc atggccgtgc cgctgggcgc gtccttcttc tggttgtggt
     3961 gcgcctcgac gatctcgaca tcgtattcgt cccccaggat ccgggcgacc tcatgcagga
     4021 ccttgaagag gatgttcacc cccacgctca tgttcggcgc cagcacgcag cggacatcct
     4081 tcgccagttt gcggatctcc tgcagctcta cggcgctgaa ccccgtcgtt ccgatcacga
     4141 tggcccgtcc gtgctgcgcg gcgatcttca catgggcaag cgaggcggca tgatgggtga
     4201 aatcgatgat cacatcgcct ttgtcgatgc acgcggacag atcgtcatgg acgatcacgc
     4261 cgggccttcc gacgccctga acttctccgg ctttgcgacc gacgagcggg tgacccttaa
     4321 cctcgaccgc gcccgacagc tcgatcccgt ccgtctggtc gatgaggctg ataatccgcc
     4381 cccccatccg tcctcccgcc gccgttacaa tggccttgac tcccatgacc tgccctccgg
     4441 atgagaaaat gtaccgggtc aaccgccgcg agggatgacg tccgcccccc tctcatcggc
     4501 ggcccgaacg cttcctatga aatcaaaccg taggccttca tgacgccccg gagcttgtcc
     4561 agattcgcgt ccgaaagccg actcagcggc agccggactt cgtcgtcgat cctgcccatc
     4621 agcttcaagg ccgccttgac ggggaccggg ttggtctcga cgaacagcgc atcaatcagc
     4681 ggggtgagct tgtggtgcag cgacctcgcc tgggccatgt cgcctgaggc ggccgcatcc
     4741 acaagcgccg ccatgtcggc tggcgccacg ttcgatgcca ccgagatgac ccccttgccc
     4801 ccgatggcca tgagcggcag ggtgaagaag tcgtcccccg acaggacgga aaacctctcc
     4861 ccgcagagcc gcacgatgtc gctcatctgt ttgagggagc cggaggcctc cttaacgccc
     4921 acgatgttgg ggatcttggc cagacgtgcc actgtctccg gcaacatgtt gacgccggtg
     4981 cgtcccggaa tgttataggg gatgatggga atcggcacgg cctccgcgat cgccttgaag
     5041 tgctggtaga gaccctcggg accggggcgg ttgtaatatg ggcagaccat cagggcgccg
     5101 tccgcgccgc attcatgggc atgccgggtg agcctgagcg cctcccgggt gctgttcgac
     5161 cccgtgcccg cgatgacggg aacgcgcttc ttcaccgcat cgatggtgat ttcgatcacc
     5221 cggtcgtgct cctcgtggga cagcgtcgcc gactcgccgg tcgtcccgca tgggacgatg
     5281 ccatccgtcc ctcccgcgat ctggaattcg atgagctccc tcagcctctc ctcgtccact
     5341 ttcccgttgt ggaacggtgt gacgatggcg acgatcgctc cctgaaacat aacgcctcct
     5401 tcaagattct tgat
//
LOCUS       DAOOLJ010000094         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_18810_length_5270_cov_17.530585, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000094 DAOOLJ010000000
VERSION     DAOOLJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18810_length_5270_cov_17.530585"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLW16_09775"
     CDS             <1..584
                     /locus_tag="PLW16_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71237.1"
                     /translation="YDVKSSAGILAPGLEMKIVDEKGEEVPMDGKSWGEILLRGPWIA
                     KEYDKDPERSKPVFAGGWLHTGDVGTIDAEGYVRLVDRTKDLVKSGGEWISSVDLENL
                     IMSHPSVLEAAVIGVPDPKWQERPVGCIVVKSGMTLTPDELKAFLKDKVKANWWIPQQ
                     FAFIDQIPKTSVGKFSKKELREMFAAGKMPLSG"
     gene            664..1260
                     /locus_tag="PLW16_09780"
     CDS             664..1260
                     /locus_tag="PLW16_09780"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPB71238.1"
                     /translation="MPISLPELPFAPDALAPVISARTLEFHHGKHHRAYVDNANKLIA
                     GTDLDAAAPEDIIQKTAGDTAKTGIFNNVAQAWNHSFYWQGIKPGGGGPPVGSVADRI
                     KAAWGDYAAFVDALKTAGTNQFGSGWVWLVADGDALKIVKTANADTPVAQGLKPLLTI
                     DVWEHAYYLDWQNRRADYLVAFIDKLINWDFVNRRLGA"
     gene            1349..2032
                     /gene="radC"
                     /locus_tag="PLW16_09785"
     CDS             1349..2032
                     /gene="radC"
                     /locus_tag="PLW16_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPB71239.1"
                     /translation="MPAERPHYHGHRKRLKERYQAQGLSGFQDYEALELFLTYAIPRG
                     DVKLLAKTLIAHFGSLKAVFDAEIAELETVKGVKRHTAILLKLSKELAGAYLKEQARE
                     RSQISCTQELYDYCLMVMGGLKDEQFRVIYLDAQNRIIDSELIEEGIVNQAVVYPRKV
                     IERALKKKASALILAHNHPSGHVRPSDADVRLTKSVQDTARLLDILVHDHIIIGENRF
                     FSFREEGMM"
     gene            complement(2242..2523)
                     /locus_tag="PLW16_09790"
     CDS             complement(2242..2523)
                     /locus_tag="PLW16_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB71240.1"
                     /translation="MNKSGLIEALSRKENLTEKKAIDVVNLVFKGFAAELKKNGRIEI
                     RGFGSFVVREYGAYTGRNPKTGKNIKVEPKRLPFFKVGKELKERVDKGK"
     gene            complement(2673..3395)
                     /locus_tag="PLW16_09795"
     CDS             complement(2673..3395)
                     /locus_tag="PLW16_09795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB71241.1"
                     /translation="MNPERLQKILARAGVASRRQGEEMIRLGRVQVNGAVVREPGTKA
                     DAARDEIRVDGRRIPPAEERLYLLMNKPRGYVTTLRDPEGRPTVRDLLIGVTERVFPV
                     GRLDYETEGLLLFTNDGEFSQRLSHPRYRITKTYRVKIQGRMPARDLVALKGGVNLED
                     GPFQPREVSLDKVNDRSTWLTLTLAEGRNRVIRRALEALGYHVTRLRRTAIGGLKLGT
                     LKEGEIRPLSSREVERLLSLRF"
     gene            complement(3392..3913)
                     /gene="scpB"
                     /locus_tag="PLW16_09800"
     CDS             complement(3392..3913)
                     /gene="scpB"
                     /locus_tag="PLW16_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB71242.1"
                     /translation="MEEDIAILDALIFSSETPLTVERMREILPQYDRQGILGLLKTLA
                     EHHEARQGGFGLCEIAGGWQFYSRPDLAPWVRKLKAGRPQMLSPAALETLAVVAYRQP
                     VVKADIDRVRGVDVGGTLKGLLEKRLVRMVGRKDVPGRPILYGTTKRFLEVFQLKDLT
                     ELPTLTELKDIQA"
     gene            complement(3916..4644)
                     /locus_tag="PLW16_09805"
     CDS             complement(3916..4644)
                     /locus_tag="PLW16_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB71243.1"
                     /translation="MSYEIKLDVFEGPLDLLLYLIRKNEIDIYNIPIALITRQYLEYL
                     DLMKALNLDLAGEYLVLAATLLHIKSRMLLPVPEEEDGGTEDEAGDPRAELVQQLLEY
                     QAFKEAALQLDRRTLLERDVFKRGAPPEALPGEEGEETPIEADIFTLVEAFQRMLSQL
                     DQADLMEIDLERISLADRINEIMDSLALRRDMTFAELMGEQITRQRIVYTFLAVLELM
                     KLRMIRVYQAASFAAIRIFIVGEE"
     gene            complement(4647..>5270)
                     /locus_tag="PLW16_09810"
     CDS             complement(4647..>5270)
                     /locus_tag="PLW16_09810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB71244.1"
                     /translation="KDLSTFGFLGYPVLQAADIIMYKAYGVPVGVDQLPHVELTREIA
                     RRFNFLYREIFPIPEPLLADVPKLLGIDGRKMSKSYENAIYLSDRGDVLTKKVGAMFT
                     DPQRMRKCDPGRPELCNVFAMHGLYSDPVEVEEIAVDCRRAGIGCTECKRRLGERIAE
                     GLQPIHERQEHYRQHLHEVTEIIREGNLRAAAIARQTLEEVREAVKI"
BASE COUNT         1009 a         1680 c         1581 g         1000 t
ORIGIN      
        1 tgtacgacgt caagtccagc gccggcatcc tcgcccccgg tctcgagatg aagatcgtcg
       61 acgagaaggg cgaggaggtc ccgatggacg gaaagtcctg gggggagatc ctcctgcgcg
      121 gcccctggat cgccaaggag tacgacaagg acccggagcg ctccaagccg gtcttcgcgg
      181 ggggctggct ccacacgggg gacgtgggga cgatcgatgc ggagggctat gttcgcctcg
      241 tagaccggac caaggacttg gtgaagagcg gcggggagtg gatctcgtcc gtggacctgg
      301 agaacctgat catgtcccat cccagcgtcc tggaggcggc cgtcatcggc gtccccgacc
      361 cgaagtggca ggaacggccg gtgggctgca tcgtcgtcaa gtcgggcatg accctgacgc
      421 ccgacgaact caaggcgttc ctcaaagaca aggtgaaggc caactggtgg atcccgcaac
      481 agtttgcctt catcgaccag attcccaaga cgagcgtggg aaaattcagc aagaaggagc
      541 tgcgcgagat gttcgcagcc ggcaagatgc cgctctccgg ttgagccgtt ttttccgctc
      601 aggaggcttg ccttcaccgg gcgcgatggt tacgttaaga caaaacgatc gaaaggagtc
      661 cttatgccca tctcactacc cgaactgccc tttgcccctg atgcgctggc gcccgtcatc
      721 agcgcccgca ccttggagtt ccaccacggc aagcaccacc gcgcctacgt ggacaacgcc
      781 aacaagctga tcgccggaac ggacctggac gcggccgcac cggaggacat catccagaaa
      841 acagccggag acaccgcgaa aaccggtatc ttcaacaacg tggcccaggc ctggaaccac
      901 tccttctact ggcaggggat caagccggga ggaggcggcc cgcccgtggg ctccgtagcc
      961 gaccggatca aggcggcctg gggggactac gccgccttcg tcgatgcgct caagacggcg
     1021 ggaaccaacc agttcgggag cggctgggtc tggctcgtcg ccgacgggga tgcgctcaag
     1081 atcgtcaaga ccgccaatgc cgacacaccg gtcgcgcagg gcctcaagcc ccttctcacg
     1141 atcgacgtct gggagcacgc ctattacctc gactggcaga accggcgagc ggattatctg
     1201 gtggccttca ttgacaaact gatcaactgg gactttgtca accgccgact gggtgcatga
     1261 tcgttgaagc gcgggggaag ggacccggcc gtaaaagcgc gaccgctccc cttcccccat
     1321 acctccaccc accgggaagg aaagacccgt gcccgctgaa cgtcctcact accacggcca
     1381 ccgcaagcgc ctgaaggagc ggtaccaggc gcagggcctg agcggttttc aggattacga
     1441 ggcgctggaa ctgttcctga cctatgccat cccgcgggga gacgtcaagc tcctggccaa
     1501 gaccctgatc gcgcacttcg gcagcctcaa ggcggtcttc gacgccgaga tcgccgaact
     1561 ggaaaccgtc aagggcgtga aacgccacac cgccatcctc ctcaagctgt ccaaggaact
     1621 ggctggcgcc tacctcaagg aacaggcccg ggagcggtcc cagatctcct gtacccaaga
     1681 gctgtacgac tactgtctga tggtcatggg ggggttgaag gacgaacagt tccgggtgat
     1741 ctatctggat gcccagaacc ggatcatcga cagtgaactg atcgaggagg gcatcgtcaa
     1801 ccaggcggtc gtctatcctc ggaaggtaat cgaaagggcc ctgaagaaga aggcctccgc
     1861 cttgatcctc gcccacaacc acccctcggg ccatgtcagg ccttcggatg ccgatgtgcg
     1921 cctgacgaaa tccgttcagg atacggcccg tctccttgac attctggtcc acgaccacat
     1981 catcatcgga gagaaccggt ttttcagttt ccgggaagaa gggatgatgt aggggaggaa
     2041 atgcccgccc caacgtcatt cgcgctggac gcgacggtgg tccgcctgca cgacgggcgc
     2101 ccatctcctt gcaggagcgc cgtgacgatc ggggaagggg aacgggccgc aaggcgtgca
     2161 tgcaaaaaaa agggggcgat ccgccccccg tgtccacctt ttcgaacggg atcggcaatc
     2221 gccccccgcc cgacagaatc tctattttcc cttgtcgacg cgctccttca attccttccc
     2281 aaccttgaag aagggaagcc gcttgggttc caccttgatg ttcttgccgg tcttcgggtt
     2341 gcggcccgtg taggccccgt actcccgaac cacgaagctg ccgaacccgc ggatttcgat
     2401 cctgccgttc ttcttcaact ctgcggcgaa ccccttgaac accagattga ccacgtcaat
     2461 cgcctttttc tccgtcaggt tctcttttct gctcagcgcc tcgataagtc cggatttatt
     2521 catgatggcc tcctcaaatg gcttgatcaa cacgcctcag agtcgttcag gcagagggcg
     2581 ttttatgaat aacacgataa tttgctttta tttttgggcg actattattt atttttcacc
     2641 gattgtcaag acaaatttta agaattcttc tattaaaacc gcaatgatag taaacgctcg
     2701 acctcccggg aagacagggg gcggatctct ccctccttca gcgtcccgag tttcagaccg
     2761 ccgatggcgg tccgccgcag tcgagtcacg tggtagccta aagcctccag ggcgcgccgg
     2821 atcacgcggt tccgcccttc cgcgagggtc agggtcagcc aggtgctccg gtcgttgacc
     2881 ttgtccagac tgacctcccg cggctggaac ggtccgtcct ccaggttcac gcctcccttg
     2941 agagccacga gatcccgggc aggcatgcgc ccctggatct tgacccggta ggttttggtg
     3001 atgcgatagc gggggtggct caggcgctgg gaaaattcac cgtcgttggt gaacagcaac
     3061 aggccttccg tctcgtaatc caggcggccc acgggaaaca cccgttcggt cacgccgatc
     3121 aggagatcgc gtacggtcgg ccgcccctct ggatcgcgca gggtcgtcac gtaaccccgc
     3181 ggcttgttca tcaggaggta gagccgttcc tcggccggag ggatccggcg accgtccacg
     3241 cgaatctcgt cacgtgccgc gtccgctttc gttcccggct cgcggacgac ggcgccgttg
     3301 acctgcaccc gccccagccg gatcatctct tccccctggc gacgggaggc cacaccagcg
     3361 cgggcgagaa tcttctggag acgttcgggg ttcacgcctg gatgtccttg agttccgtca
     3421 gggtgggaag ctccgtcaag tccttgagct ggaagacctc tagaaagcgt ttcgtcgtcc
     3481 cgtagaggat cggccggccg ggaacgtcct tgcgccccac catcctgacc aagcgctttt
     3541 cgagcagtcc cttgagcgtc cctcccacgt ccaccccgcg gacgcggtcg atgtccgcct
     3601 tgacgaccgg ctgccggtag gcgaccaccg ccagggtttc caatgccgcc ggagagagca
     3661 tctgcggccg cccggccttg agcttccgca cccagggggc cagatcgggg cggctgtaga
     3721 actgccagcc gccggcgatt tcgcacaggc cgaagccgcc ctggcgcgcc tcgtgatgtt
     3781 ccgccagggt cttaagaagg cccaggatgc cctgccggtc gtactggggc aggatttcgc
     3841 gcatccgttc gaccgtcagc ggcgtttcgg aggaaaaaat cagggcatcg agaatggcga
     3901 tatcttcttc cacggtcact cctccccgac gataaagata cggatggcgg cgaacgacgc
     3961 ggcctgatag acgcggatca tcctgagctt catcagttcc agaaccgcca ggaacgtgta
     4021 cacaatccgc tgccgggtga tctgttcgcc catgagttcc gcaaacgtca tgtcccgcct
     4081 cagggcgaga ctgtccatga tttcgttgat gcgatccgcg agcgaaatcc gctccaggtc
     4141 gatttccatc aggtcggcct ggtcgagctg cgagagcatc ctctggaagg cctccacgag
     4201 ggtgaagatg tcggcctcga tgggcgtctc ttcgccctct tcacccggca gggcctccgg
     4261 cggcgcaccg cgcttgaaga catcccgctc gagaagggtc cggcgatcga gttggagcgc
     4321 cgcctccttg aaggcctggt attcgagcaa ctgctgaacc agctccgcac gcggatcccc
     4381 cgcctcgtcc tccgtgcccc cgtcctcctc ctccggcacc ggcaggagca ttctggattt
     4441 gatgtgcagg agcgtggcgg ccaggacgag gtattccccc gccagatcca ggttcagggc
     4501 cttcatcagg tcgagatact ccaggtactg acgggtgatc agggcgatgg gaatgttgta
     4561 gatatcgatc tcgttcttcc ggatcagata gagcagcaga tccagcggtc cctcaaagac
     4621 gtcgagcttg atttcatagg acatgatcag atcttcacgg cttcccggac ttcctccagg
     4681 gtctgccggg cgatggcggc ggcccggaga tttccctccc gaatgatttc cgtcacttca
     4741 tggagatgct gccgataatg ttcctgtcgc tcgtggatcg gttgaaggcc ctccgcgatc
     4801 cgttccccca agcggcgctt gcactcggtg cagccgatcc ccgcccggcg gcagtcgacg
     4861 gcgatctcct ccacctcgac cggatcgctg tacaacccgt gcatcgcaaa gacgttgcac
     4921 agctccggcc gccccgggtc gcacttccgc atccgctgcg gatcggtgaa catggcgccg
     4981 accttcttcg tgagcacgtc tccgcggtcg ctcaggtaga tcgcgttctc gtaggacttg
     5041 ctcatcttgc gtccgtcgat gccgaggagc ttggggacat ccgcgagcag cggctcggga
     5101 atggggaaga tctcgcggta caggaaattg aaccgccgtg cgatctcccg ggtcagttcc
     5161 acgtggggga gctgatcgac gcccaccggc acgccgtagg ccttgtacat gatgatgtcg
     5221 gccgcctgaa ggacggggta gccgaggaat ccgaacgtgg acaggtcctt
//
LOCUS       DAOOLJ010000095         5197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_19154_length_5197_cov_16.899067, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000095 DAOOLJ010000000
VERSION     DAOOLJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19154_length_5197_cov_16.899067"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="groES"
                     /locus_tag="PLW16_09815"
     CDS             complement(<1..262)
                     /gene="groES"
                     /locus_tag="PLW16_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB71245.1"
                     /translation="MKVRPLHDRVIVERVGEEQKTAGGIIIPDTAKEKPQEGKVIAVG
                     PGRTDKDGKLIPMDVKKGDRILFGKYSGTEIKIDGTEYLIMRV"
     gene            615..1685
                     /gene="mnmA"
                     /locus_tag="PLW16_09820"
     CDS             615..1685
                     /gene="mnmA"
                     /locus_tag="PLW16_09820"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB71246.1"
                     /translation="MQSRERVWVAISGGVDSSVAAWLLKKQGYEVTGVTMRLRLREES
                     ALTRRCGRDAIADAARVCEVIGIPHETVDFAPALETEVIAKFVREYLRGRTPNPCIDC
                     NRSIKFGLLLAQARARGFRFLATGHYARIEETGGRHRLLRGHDRSKDQTYFLYPIPVS
                     DLGSILFPLSDLTKTEVRELARQASLPVAEKPESQDLCFVPTGGYGKLIREWGGAVHP
                     GPIVDRQGRVLGEHRGTIHYTIGQRTGLKISAPRPLYVTGIDAAGNRIFVGEKAEVRA
                     PGLVAGGMNWFVPECPPEADVKIRYRKRAVPGRISPDGEGIRIDFAEPQEAVTPGQAV
                     VLYRGDEVLGGGVIDAVLPAVG"
     gene            1818..2819
                     /locus_tag="PLW16_09825"
     CDS             1818..2819
                     /locus_tag="PLW16_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPB71247.1"
                     /translation="MSPFRLLLPVILAMFLCLPAAPRAGDPPEIRIAVLSFDNESLTD
                     SVELEPFRRGIADALVCGLSGEKGFRVIERTRIEALLSELKLNASGLVDPESTQRLGR
                     ILGVQALVLGSFAVVENEVRIDARIAEVETGHVLTAEEVSGEVEDFFDLEQALVDGIA
                     SGFRQRYSARASAAVKQGSSGKMEAGGRTGTVSTRETGAPHRLAVFPFEDLQERSGPG
                     KWGEGIAAGVAGCLSKDPHVEIVDRNRLREVVDGLALKTKDPLGERAMTRAGKIVGAN
                     VLVLGSFLSFQSRIKILVRLVKTETGEVIAAARATGRIGDRSKLEEKLAANIIRSEW"
     gene            2853..4073
                     /locus_tag="PLW16_09830"
     CDS             2853..4073
                     /locus_tag="PLW16_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB71248.1"
                     /translation="MRKTARCSGGALLIILLLSGCQTILTHVKATKAMHDGRYEESVA
                     LFQEVLKKNDNNAEALKGLGETYLRMKKAPEAVAPLEKAHQLYPSDKVAGINLGLAYN
                     MIGEHDKALQVWRPYVEKEPDTNLSIVIRKQMTLALYKDAAQKAKEALKNETALRAAL
                     KASPGDPDTLAVTYFGDKGLTEQARPLQKAIAAMLITDLSKAQSLKVVERIRVQKLLE
                     ELRLGGSGIVDKRTAPRVGRLIGAGKIVTGNMLTAGNDKMDVKGLLTNVPTGGEIGRQ
                     DAGGPVKEFFKIEKAIAFGILKDLGVQLSRKEEETIGRYATQSYPGLLYFGEGLDAQD
                     RGEWDKAIEIFQKCLKEDPTGPCSVALLDAPSGNDVEASVDAAAMASAMGTAQAEDAS
                     AAASSSGGGDGGGC"
     gene            complement(4191..4637)
                     /locus_tag="PLW16_09835"
     CDS             complement(4191..4637)
                     /locus_tag="PLW16_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71249.1"
                     /translation="MSNQYERFIVRKPTLVSELPDHDFSQAIPYPVLMGKELVPEARA
                     WALYLFIKEITQELKDLAIGTDRATPHKHAFDEMYLMIGEEKAITFEVIMGNETYRVE
                     TPAAVYIPSGVPHGIRPVDATVGKTGGLIPVCLNGEYITLPVDEPG"
     gene            4884..>5197
                     /locus_tag="PLW16_09840"
     CDS             4884..>5197
                     /locus_tag="PLW16_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71250.1"
                     /translation="MKRTVAGIFSLIAVLALWGAAPLDAREPALRIVHTNDLHSHLLG
                     FAPEIDYRPDRTGADRTVGGWARLATAIAREKAASPHPVLVLDGGDFLMGSLFHTISR
                     EAA"
BASE COUNT          977 a         1562 c         1718 g          940 t
ORIGIN      
        1 cgaccctcat gatgaggtat tccgtgccgt cgatcttgat ctccgtgccg gagtatttgc
       61 cgaacagaat ccggtctccc ttcttcacgt ccatcggaat cagttttccg tccttgtccg
      121 ttcggccggg gccgacggcg atgacctttc cctcctgagg tttttccttg gccgtgtcgg
      181 gaatgatgat gcccccggcc gttttctgct cctctccgac cctctccaca atgacgcgat
      241 catgcagtgg tctgaccttc atgcctttct acctccttga taaaaatgat gatccgatga
      301 tgctgacagc ggccgatgcc ttgccggaat gaccttaaat ttatgcatga aaacgacact
      361 gtcaagggcg tacgggtaaa tttcaggcgg cgggagaccg gcagcaaccg caaccgtcga
      421 tttcaacacc cccggccctg cctgcgctcc ccgggagaaa tagttgttga caaaaccttg
      481 ggcatattga atttttgcgc gagcgggttc gttaccggaa cggcggcggg ggcaggcgcg
      541 aggcccccgc ttccgcgccg cgtcccccga tccgtgcccg ccgggcaaac cggaccggga
      601 gagaggagac ccccgtgcag tctcgggaaa gggtttgggt ggcgatcagc ggcggcgtcg
      661 attcctccgt ggccgcctgg cttctcaaaa agcagggata cgaggtgacg ggcgtaacga
      721 tgcgcctgcg cctccgggaa gaaagcgccc tcacccggcg ctgcggtcgg gacgcgatcg
      781 cggatgccgc gcgggtctgc gaggtcatcg ggatcccgca cgaaaccgtg gatttcgcgc
      841 cggccctgga gacggaggtc atcgccaagt tcgtccggga gtacctccgc ggtcggaccc
      901 cgaatccgtg catcgattgc aaccgttcga tcaaattcgg cctcctcctg gcccaggcgc
      961 gcgcgcgggg cttccgcttc ctggccacgg gacattacgc ccggatcgaa gagaccggcg
     1021 gccggcatcg cctgctgcgc ggacatgacc ggagcaagga ccagacctat ttcctctacc
     1081 ccataccggt atcggatttg ggctcgatcc tctttcctct gtccgacctg acgaagacgg
     1141 aggtccggga actcgcccgg caggcatcgc tgcccgtcgc ggagaaaccg gagagccagg
     1201 atttgtgctt tgtccccacc ggcggttacg gcaaactgat ccgggaatgg ggtggcgccg
     1261 tccatcccgg ccccatcgtg gaccggcagg ggcgcgtctt gggcgagcat cggggaacga
     1321 tccactacac catcggccag cggacggggc tcaagatcag cgccccgcgt cccctctatg
     1381 tcaccggcat cgacgccgcc ggcaaccgga tcttcgtagg ggaaaaggcg gaggtccggg
     1441 cgccggggct cgtcgccggc gggatgaact ggtttgtccc ggagtgcccg ccggaggcgg
     1501 acgtgaaaat ccgctaccgg aagcgggccg ttcccggacg gatctcgccg gacggcgagg
     1561 ggatcaggat cgacttcgcc gagccccagg aggccgtcac cccgggtcag gccgtggtcc
     1621 tctaccgggg cgacgaggtc ctgggcggcg gggtgatcga cgcggtcctg ccggccgtgg
     1681 gctgaacgac ggcggccgat gaccaccccc gcgcggcgaa ggtctccgga gacacgcccc
     1741 ctcagacatt cgttgacata accggttatt ttatcataca ctctcagcca atgggacgga
     1801 catccacaca tttcgccttg tcaccgtttc gcttgctcct gccggtcatc ctggcgatgt
     1861 ttttgtgtct gccggcggcg ccgcgggccg gggacccgcc ggaaatccgg attgccgtcc
     1921 tgtctttcga caacgaatct ctgaccgatt ctgttgaact cgaaccgttc cgcaggggaa
     1981 tcgccgatgc gctggtctgt ggtctgagcg gggagaaggg cttccgggtg atcgagcgca
     2041 cccgtatcga ggcccttctt tccgagctga agctgaatgc ctcgggcctt gtcgatccgg
     2101 agtccacgca gaggctcggc cggatcctcg gggtgcaggc gctggtcctc ggtagtttcg
     2161 ccgtcgtcga gaacgaggtg aggatcgatg cccggattgc cgaggtggaa acgggacatg
     2221 tcttgacagc cgaggaggtc tcgggagagg tagaggattt ttttgacctc gagcaggcgc
     2281 tggtcgacgg gatcgcctcg gggttcagac aacgatattc cgcaagggcg agcgcggccg
     2341 tcaaacaggg ctcgtccggg aaaatggaag cgggcgggcg caccggaacg gtctcgaccc
     2401 gggaaaccgg ggcgccgcat cgcctcgccg tctttccgtt cgaggatctg caggaacgtt
     2461 cggggcccgg gaaatggggc gaggggattg cagcgggcgt ggcgggatgc ctgtccaagg
     2521 acccgcatgt ggagattgtc gacaggaatc gtctgagaga ggttgtcgat ggactcgccc
     2581 tgaagacaaa agaccctctc ggcgaaagag cgatgacgcg tgcaggaaag attgtgggtg
     2641 cgaacgttct tgttttggga tcgtttcttt catttcagag ccggatcaaa atcctggtcc
     2701 gcttggtgaa gacggagacg ggcgaggtca tcgccgcagc ccgagccacg ggcagaatcg
     2761 gcgaccgatc gaagctggag gagaaactgg cggcgaatat catccggtcg gagtggtgag
     2821 acagcttcgg ccaaggaaag gaggtctatg cgatgagaaa gacagcccgt tgtagcggtg
     2881 gggcgcttct gatcatcttg ttgctttccg gttgtcagac gatcttgacc cacgtgaagg
     2941 cgaccaaggc catgcacgac ggccggtatg aagagtcggt cgccctcttt caagaggtct
     3001 tgaagaaaaa cgacaacaac gcggaagccc tgaagggcct cggggaaacg tacctgcgca
     3061 tgaagaaggc gccggaggcg gtagccccgc tggaaaaggc tcatcagctc tatccaagcg
     3121 ataaggtggc aggcatcaat ctcggcctcg cctataacat gatcggggaa cacgacaagg
     3181 ccctgcaggt ttggcgacct tacgtggaaa aggagccgga caccaacctc tccatcgtga
     3241 tccgcaagca gatgacactc gccttgtaca aggacgcagc ccaaaaggcg aaggaagcct
     3301 tgaagaacga aaccgcgctg cgggccgcac tcaaggcctc cccgggagat cccgacacgc
     3361 tggccgtgac ctatttcggc gacaaggggc tgaccgaaca ggccaggccc ctgcagaagg
     3421 cgattgcggc catgctgatc acggatctga gcaaggcgca atccttgaag gtcgtggagc
     3481 gcattcgcgt tcaaaagctt ttggaagagc ttcgactggg cgggagcggc atcgtggaca
     3541 agaggacggc gccacgggta ggccgactta tcggtgcggg aaagatcgtg acaggcaata
     3601 tgctgaccgc cggcaatgac aagatggacg tcaagggcct cttgacgaac gtgccgacgg
     3661 gaggggaaat cggcaggcag gacgcgggcg gacctgtcaa ggaattcttc aagatcgaaa
     3721 aggcgatcgc ctttggcatt ctgaaggatc ttggggttca actctccagg aaagaagagg
     3781 aaacgatcgg tcgctacgca acccagtctt atccgggact tctgtacttc ggcgaggggc
     3841 tcgatgcgca ggaccgggga gaatgggata aggcgatcga gatctttcag aaatgcctga
     3901 aagaagaccc gacgggtccc tgttccgttg ccctgctcga cgcgccttcc gggaacgatg
     3961 tcgaagcgtc ggtcgacgcc gcggcgatgg ccagtgccat gggcaccgcg caagcggaag
     4021 acgcctccgc ggccgcgagc agttccggcg gcggagacgg gggcgggtgt taatcgtgga
     4081 accttggccc gccgttcgcg ggtcgggacg acagaggggt ccacgccacg gaggacatcc
     4141 cggtcccggg atgtcctgaa ggggtatcgc tgtcggcctt cgcgggcgcc ttaccccggc
     4201 tcgtcgacgg gcagggtgat gtattccccg ttcaggcaga cggggatcag tccgcccgtt
     4261 ttccccacgg tcgcatcgac ggggcggatg ccgtgcggaa cgccgctggg gatatagacg
     4321 gcggccggcg tctcgacccg gtaagtctcg ttgcccatga tcacttcgaa cgtgatggcc
     4381 ttctcctcgc cgatcatcag gtacatttcg tcgaaggcgt gcttgtgcgg cgtcgcccgg
     4441 tccgtcccga tggccaggtc cttgagttcc tgggtgatct ccttgatgaa caggtagagc
     4501 gcccaggccc gggcctctgg aaccagttcc ttgcccatca gcacggggta gggaatggcc
     4561 tggctgaagt catgatccgg cagttcgctg acaagcgtgg gtttccgcac gatgaaacgc
     4621 tcatactggt tgctcatggt tttccctcct ccgcaatcgg tagcccaagg tatcagacgg
     4681 cttggggttt gtccaccgga atgcggaccc gacctccccg cgtccggcga cggggccgga
     4741 cccgaatcga agacgacctg ccgggagacc gttctccctt gacgtcccgg ggcggaaagg
     4801 atagggattg ggcatccgca ccccgcgaac gccgccacgg cgtgataccg accccggggt
     4861 tcccgacctt taaggaggat gtgatgaaaa gaacggtggc gggcatcttc agtctgatcg
     4921 ccgtgctggc cctgtggggc gccgcgccgt tggacgcccg ggaaccggcc ttgaggatcg
     4981 ttcatacgaa cgacctccac tcgcacctcc tgggatttgc cccggagatc gactaccggc
     5041 cggaccggac gggcgccgac cgcaccgtcg gcggctgggc gcgcctcgcc acggcgatcg
     5101 cccgggagaa ggccgcgagc cctcaccccg tgctcgttct cgacggcggc gactttctca
     5161 tggggtccct cttccacacg atcagccgcg aggcggc
//
LOCUS       DAOOLJ010000096         5051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_19931_length_5051_cov_23.103683, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000096 DAOOLJ010000000
VERSION     DAOOLJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19931_length_5051_cov_23.103683"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..746)
                     /locus_tag="PLW16_09845"
     CDS             complement(180..746)
                     /locus_tag="PLW16_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPB71251.1"
                     /translation="MTPPLPDRIPTREECDALMARYAMLPNIVAHSRQVMRVSLAIAD
                     HLQSGVSVNRDLVVAAALLHDITKTRSLETHERHDASGGALLREMGFARTAEIVEQHV
                     LLRDVDLDGGLEEREIVCYADKRVRHDAVVTLEERIQDLIARYGDSEEIRRLIRVNSS
                     QVFALERKIAGFMTVGIEEAIRSQEDAG"
     gene            complement(743..1891)
                     /gene="leuB"
                     /locus_tag="PLW16_09850"
     CDS             complement(743..1891)
                     /gene="leuB"
                     /locus_tag="PLW16_09850"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPB71252.1"
                     /translation="MTDRRFRIAIFPGDGIGPEVIRQAVRVLEIVARHTGTALDLTEG
                     LVGGAAYDRCGTPLPPESLDQALASDAVLLGAVGGPSWEALPYDVRPERALLGLRERL
                     GLFANLRPVVAFEDLIDASPLKAEIVRGTDILILRELTGGVYFGRPRGIRVEDGRRVG
                     VNTMIYAEEEIRRIAVKAFETARGRRKRVVSVDKANVLESTELWRDVITEVGRGYPDV
                     ALRHLYVDNCAMQLVLNPAQFDVIVTTNLFGDILSDEAAMLTGSIGLLPSASLGDRHA
                     LYEPIHGSAPDIAGQDLANPLAAILSVAMMLEHTLGLPGEARRVEDAVRTVLREGRRT
                     RDLNRERATPVGTAEMGEAVCRTLERLLTAANGPEGQDAAGRGDSRSS"
     gene            complement(1888..2406)
                     /locus_tag="PLW16_09855"
     CDS             complement(1888..2406)
                     /locus_tag="PLW16_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPB71253.1"
                     /translation="MKTLQGRIWTFGDNIDTDAIIPARYLNTSDPASLAAHCMEDADP
                     DFMKKMRPGDILVAGENFGCGSSREHAPIAIKAAGIACVVAKSFARIFYRNAFNMGLP
                     IFESGELHDRVTDGDEIEVDADRGLIRIGPDGPPLKIEPIPPFMQQLLTDGGLMGHLA
                     RKAAGRAERSPA"
     assembly_gap    2603..2702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2703..3547)
                     /locus_tag="PLW16_09860"
     CDS             complement(<2703..3547)
                     /locus_tag="PLW16_09860"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPB71254.1"
                     /translation="MGMTITEKILCRHTHLDRVHPGELISARVDLALANDITAPIAIA
                     EFRRAGGTKVFDRNKIALVCDHFTPNKDIESAGQCRTVRAFAREQAIDHFYEGGNCGV
                     EHALLPEQGVVLPGEVVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGEAWFKVPES
                     MKFVLTGALKPWVSGKDLILHIIGRIGVDGARYRAMEFSGETVGALTMADRLTMANMA
                     IEAGAKNGIFPVDDTTRAYVAARSDRRYVAGPPDPDADYRDVLEIDCSAIEPQVAFPH
                     LPSNVR"
     gene            complement(3592..>5051)
                     /locus_tag="PLW16_09865"
     CDS             complement(3592..>5051)
                     /locus_tag="PLW16_09865"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPB71255.1"
                     /translation="EKLLIARQLERLGVDIIEAGFPVASEGDFDSVRRIADEVRGVQI
                     AALARANEADIDRAWEAVAGAARPRIHTFISSSDIHLEYQLRKTRAEVLAAAVDAVAR
                     ARSYTPNVEFSPMDATRTDRNYLCEMVEAVIAAGATTVNIPDTVGYAVPEEFGDLIAT
                     LFDRVPNIAQAIVSVHCHNDLGLAVANSLAAIRNGARQVECTINGIGERAGNTAMEEL
                     VMALATRRDRFALTTGIRAEQIFPTSRLLTKITGIPVQPNKAIVGANAFAHESGIHQD
                     GLIKERRTYEIMTPQSVGIAETRIVLGKHSGRHAVAEHLRAMGYALTEEEVNRVFVRF
                     KELADIKKDVYDEDLEAILYEAVFRVEDHYKLVYLHVTSGTTAVPAATMQMEVDGRLV
                     QDGGFGVGPVDATFAAIRKITQTNHPLEKYLVHAVTGGTDAQGEVTVQLKYNGRSAVG
                     HSAHPDVLVASAKAYINALNRIEFLKANVRKVDVE"
BASE COUNT          699 a         1635 c         1735 g          882 t
ORIGIN      
        1 cgacctcctt gagacgatga gggctggctt gcaggtttgg gggtgcggcg gcatcccggc
       61 gggtgcaccg ggcgggtgcg tgtccccgcc tgtttgcgcc tcgcttcgag gcagtatagt
      121 ccatcctcga aaaaaatcaa ttttttcggt cgcggcgccc ggcgggaggg tccgcctccc
      181 tatcccgcgt cctcctggct gcggatggcc tcctcgattc cgaccgtcat gaaaccggcg
      241 atcttgcgct cgagggcgaa gacctggctg ctgttgacgc gtatcagccg gcggatctcc
      301 tccgagtcgc cgtagcgcgc gatcaggtcc tgaatccgtt cctcgagagt cacgacggcg
      361 tcgtgccgca cccgtttgtc ggcgtagcac acgatctccc gctcctccag cccgccgtcc
      421 agatccacat cccggaggag gacatgctgt tccacgatct ccgccgtgcg ggcaaatccc
      481 atttcccgca ggagggcgcc gccggaggca tcgtggcgct cgtgggtttc gagcgaccgg
      541 gtcttcgtga tgtcgtgcag gagggcggcc gccaccacga ggtcccggtt cacggacacg
      601 ccgctttgca ggtggtccgc gatcgccagg gagacgcgca tgacctgacg ggagtgcgcc
      661 acgatgttcg gcagcatggc ataccgcgcc atgagcgcgt cgcactcttc ccgcgtcggg
      721 atcctgtccg ggaggggggg cgtcatgatg atcttgaatc gcctctcccc gcggcatcct
      781 gcccttcggg cccgtttgcg gccgtgagca gccgttccag ggtccgacag accgcctcgc
      841 ccatctcggc ggtgccgacg ggcgtcgccc tctcccggtt aagatcgcgg gtgcgccgcc
      901 cctcccgcag gaccgtgcgg accgcgtcct cgacgcggcg cgcctctccc ggcaggccga
      961 gggtgtgttc gagcatcatc gcgacggaga ggatcgcggc caggggattg gccaggtcct
     1021 gccccgcgat gtcgggggcc gaaccgtgga tcggttcgta gagggcatgc ctgtctccca
     1081 ggctcgccga ggggagcaga ccgatggaac cggtcagcat ggcggcctcg tcgctcagga
     1141 tgtctccgaa gaggttcgtc gtgacgatca cgtcgaactg ggcggggttc aggacgagct
     1201 gcatcgcgca gttgtcgacg tacaggtgcc ggagggcgac gtccgggtag ccgcggccga
     1261 cctccgtgat gacgtcccgc cagagttccg tcgactccag gacgttcgcc ttgtccacgg
     1321 agacgaccct cttccgccgt ccgcgggcgg tctcgaaggc ctttaccgcg atgcgccgga
     1381 tctcctcctc cgcatagatc atcgtgttga cgccgacgcg ccggccgtcc tcaacccgga
     1441 tgccccgagg ccgcccgaag tagacgccgc cggtcagttc ccggaggatc aggatgtctg
     1501 tcccgcggac gatctcggcc ttgaggggcg aggcgtcgat caggtcctcg aaggcgacga
     1561 ccggccgcag gttcgcgaag agtcccagcc gctcccttag gccgaggagg gcccgctccg
     1621 gacggacgtc gtagggcagg gcctcccagg acggcccgcc cacggcgccc agcagcacgg
     1681 cgtcgctcgc cagcgcctga tcgaggctct ccggggggag cggcgtgccg cagcggtcat
     1741 acgccgcgcc gccgacgaga ccctcggtca aatccagggc cgtgcccgtg tggcgagcga
     1801 cgatctcaag cacgcggacg gcctgccgga tcacctccgg tccgatgccg tcgccgggaa
     1861 agatggcgat gcgaaagcgg cggtcggtca tgcgggactc ctctccgccc gccccgccgc
     1921 cttccgggcg agatggccca tcaggccccc gtccgtgagg agctgctgca tgaagggtgg
     1981 gatcggctcg atcttcagcg gcgggccgtc gggcccgatg cgaatcaggc cccggtccgc
     2041 gtccacctcg atctcgtcgc cgtcggtcac ccggtcgtgc agctcgcccg attcgaagat
     2101 cggcaggccc atgttgaagg cgttccggta gaaaatgcgt gcgaaactct tcgcgacgac
     2161 gcaggcgatg cccgccgcct tgatcgcgat gggggcgtgc tcccgggaag agccgcagcc
     2221 gaaattctcc cccgccacga ggatgtcgcc cggcctcatc tttttcatga agtccggatc
     2281 ggcgtcctcc atgcagtgcg ccgcgaggct ggccgggtcc gaggtgttga ggtagcgcgc
     2341 cgggatgatg gcgtccgtgt cgatattgtc gccgaatgtc cagatccggc cttggagggt
     2401 cttcatgggg ttcgtctcct gaaatctgca tgcagcgtcc ggccgggccc cgcgccgccg
     2461 cgggagtggc gggggcttcg tctcacgtca ggtcgtccgg tccggcgagg cggcccagga
     2521 cggccgaggc ggcggcgaca gcggggttcg ccaggtagac ctcgctctcg cggtggccca
     2581 tccggccgac aaagttgcgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nncggacgtt cgagggcagg tggggaaagg cgacctgggg ctcgatggcg ctgcagtcga
     2761 tctccaggac gtcgcggtag tcggcgtcgg ggtcgggcgg gccggcgaca tagcgccggt
     2821 cgctccgcgc cgccacgtag gcacgggtgg tgtcgtccac ggggaagatc ccgtttttcg
     2881 ccccggcctc gatggccatg ttggccatgg tgaggcggtc ggccatcgtg agcgcgccca
     2941 cggtctcgcc cgaaaactcc atcgcgcggt agcgggcgcc gtccacgccg atccgtccga
     3001 tgatgtgaag gatcaggtcc tttcccgaca cccagggttt gagcgcgccg gtcaggacga
     3061 acttcatcga ctccgggacc ttgaaccagg cttccccggt cagcatggcc gccgccaggt
     3121 cggtactgcc cacgcccgtg gcgaaggccc ccagcgcgcc gtaggtgcac gtgtggctgt
     3181 cggcgccgat gacaacctct ccgggcagga cgacgccctg ttccggcagg agggcatgct
     3241 cgaccccgca gttgccgcct tcgtagaaat ggtcgatcgc ctgttcgcgg gcgaacgcgc
     3301 ggacggtccg gcactgtccg gccgactcga tgtccttgtt cggggtgaag tggtcgcaga
     3361 ccagggcgat cttgttccgg tcgaagacct tcgttcctcc cgcccggcgg aattcggcga
     3421 tggcgatggg ggcggtgatg tcgttcgcca gggcgaggtc cacccgggcg ctgatgagtt
     3481 cgccggggtg gacacggtcg aggtgcgtgt gccggcagag gatcttttcg gtgatcgtca
     3541 ttcccatggg gtcgtgtcct ttccgtgctt tccttatccg atcgctccga tttattcaac
     3601 gtcaaccttc cggacgttgg ccttgaggaa ctcgatgcgg ttgagcgcgt tgatgtaggc
     3661 cttcgccgag gcgaccagga cgtccggatg ggcgctgtgc cctacggcgg agcgaccgtt
     3721 gtacttgagc tggaccgtga cctctccctg ggcgtccgtc ccgcccgtga cggcgtggac
     3781 aaggtacttc tccaggggat ggttcgtttg cgtgatcttc cggatggcgg cgaaggtcgc
     3841 gtccacgggg ccgacgccga acccgccgtc ctggacgagc cggccgtcca cctccatctg
     3901 catcgtcgcg gccgggacgg cggtggtgcc gctcgtcacg tgcagataga cgagcttata
     3961 gtggtcctcg acccggaaga cggcctcgta aaggatggcc tccaggtcct cgtcgtagac
     4021 gtccttcttg atgtccgcca gttccttgaa ccgcacgaaa acccggttca cctcctcctc
     4081 cgtcagggcg taccccatcg ctcggaggtg ttccgccacg gcgtggcgac ccgagtgctt
     4141 gcccaggacg atccgggtct ccgcgatgcc caccgactgg ggggtcatga tctcgtaggt
     4201 ccgcctttcc ttgatcaggc cgtcctggtg gatccccgac tcgtgcgcga aggcgttggc
     4261 gccgacgatg gccttgttcg gctggacggg gatgccggtg atcttcgtca gcaggcggga
     4321 ggtcgggaag atctgttccg cccggatgcc cgtggtgagc gcgaagcggt cccggcgggt
     4381 cgccagggcc atgacgagct cctccatggc cgtgttgccg gcccgttcgc cgataccgtt
     4441 gatcgtgcac tccacctggc gggcgccgtt gcggatcgcg gccagggagt tggccacggc
     4501 gaggcccagg tcgttgtggc agtggacgga aacaatcgcc tgtgcgatgt tcgggacgcg
     4561 gtcgaagagc gtcgcgatca ggtcgccgaa ctcctccggg acggcgtagc cgacggtgtc
     4621 ggggatgttg accgtcgtcg cgccggcggc gatgaccgcc tccaccatct cgcacaggta
     4681 attccggtcg gtgcgcgtgg cgtccatcgg cgagaattcc acgttgggcg tataggaccg
     4741 cgcgcgggcg acggcgtcga cggccgcggc gaggacctcg gcgcgggttt tcctgagctg
     4801 gtattccaga tgaatgtccg aggaggagat gaaggtgtgg atccggggac gggccgcccc
     4861 cgcgacggcc tcccaggccc ggtcgatgtc ggcctcgttg gcgcgggcca gggcggcgat
     4921 ctggacgccc cggacctcgt cggcgatcct gcggacggaa tcgaagtcgc cctccgaggc
     4981 gacggggaac cccgcctcga tgatgtccac gcccaggcgt tcgagctgcc gggcgatcag
     5041 gagtttttcg c
//
LOCUS       DAOOLJ010000097         4848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_21051_length_4848_cov_17.391613, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000097 DAOOLJ010000000
VERSION     DAOOLJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21051_length_4848_cov_17.391613"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..83)
                     /locus_tag="PLW16_09870"
     tRNA            complement(9..83)
                     /locus_tag="PLW16_09870"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Thr,seq:cgt)
     gene            373..2622
                     /locus_tag="PLW16_09875"
     CDS             373..2622
                     /locus_tag="PLW16_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPB71256.1"
                     /translation="MGERIRKLFTVTPFKITVLVILIALTLFFVDVRFIKFMELKALD
                     LRMVYRGELPSGGETVIVTVDEKSLNELGRWPWPRTTVAKVVERLKAGGAKVVGFDVV
                     FAEPDENSSLKTLASLGSEMKRLGIHDRQLSSLIARKQRGADTDAALAQAIARAKNVT
                     LGYFFHIQKRDAEHMTERQISDGEKYIRNSRYATVKMTPESSDFPIVPAYAAVTNLKA
                     ISAAGQNSGYFNAFPDSDGTIRWAPLVIKFRDGYYTSLALSTLLQYLDRPMLTLNLAE
                     FGVESIQLGSIEIPTDESGRLLLNYLGPAKTFAHYSAADVYANRLPAGTFKDKIVLIG
                     ATATGIYDLRVTPFSAVYPGVEIHATAIDNILHQRFLLHSAWTMFIDILAIVLCGLLI
                     GVAVPRLRAVQGVILILAALAVFVVLNAVVFARFNVWLNLIYPVLTMMTIYLGITVYR
                     YVTEEREKKKIRGAFQYYLTASVITEMLKDPDKLKLGGDKKDLSVLFSDIRGFTTVSE
                     KLTPEELVHLLNEYLTAMTNIVFKYDGLLDKYMGDAIMAVFGAPLDQPDHALRACRTA
                     LGMMEALKTLQKKWTDEGRPVLDIGVGINSGDMVVGNMGSEMRFDYTVMGDSVNLGSR
                     LEGINKEYGTNIVISEYTYEAVKDALCCRELDMVKVKGKKLPVRIYELLGEKRDEAKW
                     RDLVRLFDEALALYRQGCWDDAIAAFRKVLEVRPGDPPSELYLERCIELRKEPLPEDW
                     DGVCTMTRK"
     gene            2634..4592
                     /gene="alr"
                     /locus_tag="PLW16_09880"
     CDS             2634..4592
                     /gene="alr"
                     /locus_tag="PLW16_09880"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB71257.1"
                     /translation="MSVRAEEKYRSWVEVDLDHFTHNGEELKRIVGPDVKILQVVKAD
                     AYGHGAIEISNVALKNGVSMLGVANADEGVQLRVSGISAPILILSPSLASEIDEIIKY
                     NLTPSVSDLGFARELDRRFRKAGVRRPVHVEVDTGMGRGGTLYEGALPMIRRIVSYPH
                     LEVEGIFTHLSSSEQLTDYNDMQWELFQRLLDELMAENIRIPVRHMSNSGGILNYPPF
                     HLDMVRPGIMSYGVYPSLETETRAYLKPVMSFKTRVVLVKDFPAGRGIGYGRSYVTER
                     PTKIATIPVGYGDGYGVILSNQGEVLIGGRRAPIVGRVSMDMCTVDVTHVEDCRIGDE
                     VVLLGRQGDESISANEIADKVKTISYEILCALGKRAPRIFLSQGMRDSVEPRLRRIFI
                     PDEERSLARIDGIIRNCFQTRARNVELGDAIYYEMFETLFGKEDRQLELRTDFRYDIT
                     VRALPPEAGGKPHARRDYFQVTTHIEYVKTIRNPVFMIGCALNNEQLEQFFDDPRCEY
                     RWLLSRGEDLVAARDFRVDAVRIDGETIPLLRAENTDRGYEVWCGGDVLRKKLNRPVK
                     VEIEIITKKSRSGNIFSVYLVYPTRGLNISFNYAGTGLKNVREVSFFAGKHPDPEVVR
                     EKGKSIALAVRADEWIFPKSGVTFIWDA"
     gene            4628..>4848
                     /locus_tag="PLW16_09885"
     CDS             4628..>4848
                     /locus_tag="PLW16_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71258.1"
                     /translation="MEKLKVGVILGGMSSEKEVSLNSGRNVHDHLDREAYEAVPIFMD
                     ADGRLWVIPWQLVSQNTTTDIVERLETEAR"
BASE COUNT          954 a         1453 c         1541 g          900 t
ORIGIN      
        1 ttgatttctg gagccggaga ggggggttga acccctgact tgctgattac gaatcagcta
       61 ctctaccact gagttactcc ggcttttttc gagttttaag ccctgtcgaa caggctttcc
      121 cgctttatac caagcacttt tccttgtcaa gcgattatcg cccgccgccg gcagggatcg
      181 gccttccggc agggtgccgc catcccgccc cgccgtgccc taacccccac gtttgccttg
      241 acattacggg gggctctgca tatgatgcgc catccaagac agcgaaggcc cgggcgtgcc
      301 ggagcaagca gatgtgcggc tgcgggcacg cctcaaccgt tggcgtcgga cactccgggg
      361 aggaggtcat ccatggggga acggattcga aaactgttta cggtcacgcc cttcaagatc
      421 acggtgctcg tcatcctgat tgccctgacg ctctttttcg tcgacgtgcg cttcatcaag
      481 ttcatggagc tcaaggccct cgatctgagg atggtgtacc gcggtgaact gccctcgggg
      541 ggcgagacgg tgatcgtcac cgtcgacgag aaaagcctca acgagctcgg gcgctggccc
      601 tggccgagga cgaccgtggc aaaggtcgtc gagcgcctga aggccggcgg ggccaaggtg
      661 gtgggcttcg acgtcgtctt cgcggaaccg gacgaaaatt ccagtttgaa gaccctggcc
      721 agtctgggca gcgagatgaa gcgcctgggg atccatgacc ggcaactgtc ctcgttgatt
      781 gccagaaaac agagaggggc cgatacggat gccgccctgg cgcaggcgat tgcccgggcc
      841 aagaacgtca ccctcgggta ctttttccac attcagaagc gggacgccga acacatgacc
      901 gagcggcaga tcagcgacgg ggagaagtac atccgcaatt cccgctacgc gacggtcaag
      961 atgacgcccg aatcgagcga ttttcccatc gtgccggctt acgccgcggt gaccaacctg
     1021 aaagccattt ccgcggcggg gcagaacagc ggctatttca acgcctttcc ggattccgac
     1081 gggacgatcc gctgggcgcc gctcgtcatc aagttccgcg acggctacta cacatcgctg
     1141 gccctctcca cccttctgca gtatctcgac aggccgatgc tgaccctgaa tctggccgag
     1201 ttcggcgtgg agagcattca gctcggttcg atcgagattc ccacggacga gtcgggccgg
     1261 ctattgctca actacctggg acccgcgaag acgtttgccc actattccgc cgcagatgtc
     1321 tacgcgaacc ggctgccggc gggaacgttc aaggacaaga tcgtcctgat cggcgcgacg
     1381 gcgacgggga tctacgatct gcgcgtgacg cccttcagcg ccgtttaccc cggggtcgag
     1441 atccatgcga cggcgatcga caacattctg catcagcgat tcctcctcca ttccgcgtgg
     1501 acgatgttca tcgacatcct cgcgatcgtc ctttgcggcc tgctcatcgg agtggccgtg
     1561 ccgcgactca gggcggtgca gggcgttatc ctgatcctgg ccgcgcttgc cgtgttcgtc
     1621 gtgctcaacg cggtcgtttt tgcccggttc aacgtctggc tcaacctcat ctatcccgtc
     1681 ctgacgatga tgaccatcta tctcggcatc acggtctacc gctacgtgac ggaagaacgg
     1741 gagaagaaga agattcgggg ggcgttccaa tactatctga ccgcttccgt catcaccgaa
     1801 atgctgaagg accccgacaa gctgaaactg gggggagaca agaaggacct gtccgtcctg
     1861 ttctcggata tccgggggtt caccaccgtt tcggagaagc tgacgccgga ggaactggtt
     1921 catctgctga acgagtacct gacggccatg accaacatcg tcttcaagta cgacgggctc
     1981 ctggacaaat acatgggaga cgcgatcatg gcggttttcg gcgcccccct ggaccagccc
     2041 gaccatgcct tgcgggcctg ccggaccgcc ttggggatga tggaggcgct gaagacgctc
     2101 cagaagaaat ggaccgacga gggccggccg gtgcttgaca ttggcgtggg gatcaactcg
     2161 ggcgacatgg tcgtgggaaa catgggctcc gagatgcgct tcgactacac ggtcatgggg
     2221 gacagcgtca acctgggttc ccggctcgaa gggatcaaca aggagtacgg aaccaacatc
     2281 gtgatcagcg aatatacgta cgaagccgtc aaggatgcgc tgtgctgccg cgaactggat
     2341 atggtgaagg tcaaggggaa aaagctgccg gtcaggatct atgaactcct gggcgagaag
     2401 cgggatgagg cgaaatggcg ggacctcgtc cgactgttcg acgaggccct ggccctttac
     2461 cggcagggat gctgggacga cgcgatagcc gccttccgga aggtcctgga ggtgaggccg
     2521 ggcgatccgc cctcggaact ttatctcgag cggtgcatcg aactccggaa ggaacccctg
     2581 cccgaggact gggacggggt ctgcaccatg acgcggaagt agccgggatc gatatgtcag
     2641 ttcgcgcgga agagaaatat cgcagctggg tcgaggtcga cctggaccat ttcacccaca
     2701 acggggagga gttgaagcgg atcgtcggtc cggacgtgaa gatccttcag gtggtcaagg
     2761 cggatgctta cggtcacggg gcgatcgaga tttcgaacgt ggccctgaaa aacggagtgt
     2821 ccatgctggg ggtggccaac gccgacgagg gcgtgcagct ccgggtcagc ggcatcagcg
     2881 ctccgatcct catcctgagc ccctcgctgg cctccgagat cgacgaaatc atcaagtaca
     2941 acctgacccc gtccgtctcg gatctcggct ttgcccggga actcgatcgg cgcttccgga
     3001 aggccggcgt ccggcgcccg gtccatgtcg aggtggacac gggcatgggg cgcggcggca
     3061 ccctttacga gggggcgctg cccatgatcc gccggatcgt ctcgtacccg catctggagg
     3121 tcgaggggat cttcacccac ctgtcctcga gcgagcaact gacggattac aacgacatgc
     3181 agtgggagct cttccagcgc ctgctcgacg aactcatggc cgagaacatc cggattcccg
     3241 tccgtcatat gtcgaacagc ggcggcatcc tgaactatcc gccgttccat ctcgacatgg
     3301 tccggcccgg gatcatgtct tacggggtct acccgtcgct cgagacggag acgcgggcct
     3361 acctcaagcc ggtcatgagc ttcaagacgc gggtggtgct cgtcaaggat ttccccgcgg
     3421 ggcgcggcat cggttacggg aggtcctacg tcacggagcg gccgacgaag atcgccacga
     3481 ttcccgtcgg ctacggggac ggctacgggg tgattctgtc caaccagggg gaggtcctga
     3541 tcggcggccg gcgcgcgccc attgtcggcc gggtttccat ggacatgtgc acggtcgacg
     3601 tgacccatgt cgaggattgc cggatcggcg acgaggtcgt cctgctcggg cgccagggcg
     3661 acgagtccat ctcggccaac gaaatcgcgg acaaggtcaa gacgatcagt tacgagatcc
     3721 tctgcgcgct gggcaagcgc gcgccgcgca tcttcctgag ccaggggatg cgggactccg
     3781 tcgaaccgcg cctgcgccgg atcttcattc ccgacgagga gcggtccctg gcccgcatcg
     3841 acggcatcat ccgcaactgc ttccagactc gggcgagaaa cgtcgaactg ggcgacgcga
     3901 tctactacga gatgttcgaa accctcttcg gcaaggagga ccggcagctc gagctgcgga
     3961 cggacttccg gtacgacatt accgtgcggg cgctgccgcc ggaggcgggc gggaagccgc
     4021 acgcgcgacg cgactatttc caggtgacga cccatatcga atacgtcaag accatccgga
     4081 accccgtgtt catgatcggc tgcgccctga acaatgaaca gctggaacag ttttttgatg
     4141 acccgcgctg cgagtaccgc tggcttctga gccggggcga ggacctcgtt gcggcccggg
     4201 atttccgggt cgatgccgtc cggatcgacg gggagacgat ccctcttctc cgggccgaaa
     4261 acaccgatcg gggctatgaa gtctggtgcg ggggcgacgt cctccggaaa aagctgaatc
     4321 ggccggtcaa ggtcgaaatt gaaatcatca cgaagaaatc gagaagcggc aacatcttct
     4381 ccgtctatct ggtctacccg acgcgtgggc tcaacatctc cttcaactat gccgggacgg
     4441 gtctcaagaa cgtccgtgag gtgagtttct ttgcgggcaa gcaccccgat cccgaggtcg
     4501 tccgcgaaaa ggggaagtcg attgccctcg cggtccgcgc cgatgaatgg atctttccca
     4561 aaagcggcgt gaccttcatc tgggatgcgt gacggacggc gggcgagcgt tgccgggagc
     4621 ggggaaaatg gaaaagctga aagtgggtgt catcctgggc ggcatgtcct cggagaaaga
     4681 ggtctccctg aacagcggcc ggaatgttca cgaccatctg gaccgggagg cctacgaagc
     4741 cgtcccgatc ttcatggacg ccgacgggcg cttgtgggtg attccctggc agctcgtttc
     4801 gcagaacacc acgacggaca tcgtggagcg cctcgaaacc gaggcccg
//
LOCUS       DAOOLJ010000098         4764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_21563_length_4764_cov_14.897643, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000098 DAOOLJ010000000
VERSION     DAOOLJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21563_length_4764_cov_14.897643"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLW16_09890"
     CDS             <1..307
                     /locus_tag="PLW16_09890"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPB71259.1"
                     /translation="FPLDADHLVRHPSQLYEALGEGVLLFLLLWAIRRRSPFDGFLFG
                     LYVIGYGTARFVIEYFREPDAQLGLFLGMFSMGQLLCLLMIAAGGLILAVRKPQAAI"
     gene            381..512
                     /locus_tag="PLW16_09895"
     CDS             381..512
                     /locus_tag="PLW16_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HPB71260.1"
                     /translation="MGKRVVIVGGGAAGPSVAAEAKRNDPSLEITMIEQGAFVSYAA"
     gene            516..1712
                     /locus_tag="PLW16_09900"
     CDS             516..1712
                     /locus_tag="PLW16_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB71261.1"
                     /translation="MPYYIGDVIRDEGTLIARTPEKFREAGIEVRLESTVTAIDVTQG
                     RVRLTNGELLPYDVLVLATGAESRLPAWPGFDLPGVFLLKNLADAIRIKAHLNRSSCR
                     KAVVIGAGFIGMEMCEALRDRGMETTVVHRGDLPVARWDAEFAREIVAALERNGVAFL
                     ADTELHGIEAGTSCPLKVMTSRGPIEADLVLVAVGIAPNVLLARDMGLRIGATGAIGV
                     DAQQQTSAPNVYAVGDCCEVYHRVSSRWAYLPLGDTANKQGRLAGRIIGGRPAAHPGV
                     VGAQSFKIFELETAATGLNEAEALQAGFDPIGDVYWGNAIAGYFGKRKLGLKLLADRA
                     SGKLLGAQAIGETGAVGRINTLSAALWSELTLDEIGWLDLAYAPPFGGAWDVIHRAAQ
                     RLQRKR"
     gene            1716..2678
                     /locus_tag="PLW16_09905"
     CDS             1716..2678
                     /locus_tag="PLW16_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPB71262.1"
                     /translation="MSEQMVYWGIFIAFVIVMLVLDLGVFNRKAHVIKVREALMWTGF
                     WVSLALLFCLGVYWVKGSEKALAFLAGYLIEESLSVDNLFVFLMIFSYFGIPPQYEHK
                     ALFWGIIGALVMRAVFIAAGVALIEQFHWIIYVFGAFLIFTAVKMARSGEGNVHPDRN
                     VALKLLRKVMPVTNDYAGGKFFVREGGRWMATPLFAVVLVLETTDVIFAVDSIPAVLA
                     ITTDPFIVYTSNVFAILGLRSIFFALAGMMRLFYYLRYGLVVVLSFVGMKMILTEWVK
                     IPVGLSLGVIAAVLAVSIVASLALPHPVRMVIDDEAGKKENPVG"
     gene            2729..3448
                     /locus_tag="PLW16_09910"
     CDS             2729..3448
                     /locus_tag="PLW16_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPB71263.1"
                     /translation="MPSPSSRAYTCECRDVKPVPAESRQALLVTAPGWNAVAATLERY
                     ERTGAGVPWLAVGERIPAVLGRNGLAWGRGLATPPLDGPVKEEGDGRSPAGVFRIRGA
                     FGYAPAADVPWIRLPYRQATAGLKCVDDHASARYNRLVDTAVEPQTWSRHEEMLRKDD
                     LYRLGAVIGHNDDPTVSGGGSCIFLHAWGGPDRGTAGCTAVALPRLEDILHWLDPALC
                     PVLVQLVVEDRERLRTAWDLP"
     gene            3571..4335
                     /locus_tag="PLW16_09915"
     CDS             3571..4335
                     /locus_tag="PLW16_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB71264.1"
                     /translation="MQALSSARVTAWAKLNQTKYRRRERLCLAEGEKVVRELLRSPWA
                     VQAVLVAEDAAARYQALVASLPSGSVHAVPARFWKRLSQDPAPEGIMAVAALPPPLAA
                     AARAPGPGPLLLIDRVNNPNNLGALLRTAHWFGFRTVLVGAGSADWTHPKVIRTSMGS
                     LFHLNVTADLDLSAVLPALRGRFRLIGSDVRRGVPPHPCPPQTALLLGNETHGLPPAL
                     LDLTDECWCIPGAGAAESLSLPQAAAVMMYEFRKGE"
     gene            4338..4667
                     /locus_tag="PLW16_09920"
     CDS             4338..4667
                     /locus_tag="PLW16_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HPB71265.1"
                     /translation="MQWVFLAVAILAEVIGTAALKVSDGFTHPVPSLVVLLGYGISFF
                     FLSLTLRTLPVGVAYAVWSGAGMALIALIGWLFLGQSLDAPAIVGLFLIGAGVAVLNL
                     YSRSVVN"
BASE COUNT          696 a         1502 c         1667 g          899 t
ORIGIN      
        1 cttccccctc gacgccgacc atctcgtccg ccatccctcg cagctctacg aggccttggg
       61 cgagggggtt ctcctgttcc tcctcctgtg ggcaatccgc cggaggagcc cgttcgacgg
      121 gttcctcttc ggtctgtacg tgatcggcta cgggacggca cgtttcgtga tcgaatattt
      181 ccgagagccg gacgcgcaac tcggcctgtt cctggggatg ttcagcatgg gtcagcttct
      241 gtgcctgctc atgatcgccg cggggggcct gatcctggcc gtccggaagc cgcaagcggc
      301 gatatagatc gacgggccgg gcagaccggc gggaccgccc gccggaccgt caacatccac
      361 aaacccaatg gaggtgcaag atggggaagc gggtcgtcat tgtcggtggg ggcgcggccg
      421 gtccatccgt ggccgcggag gccaaacgga acgatccctc gctggagatc acgatgatcg
      481 aacagggggc gttcgtgtcc tatgccgcct gaccgatgcc ctattacatc ggcgatgtaa
      541 tcagggacga aggaacgctg atagcgcgca cgcccgagaa attccgggaa gcgggtatcg
      601 aggtgcgcct ggagtcgacg gtgaccgcga ttgatgtcac ccagggccgt gtgcggctga
      661 ccaacgggga gttgctgcct tacgacgttc tggtcttggc gaccggcgcc gagtcccggc
      721 ttccggcgtg gccgggattc gacctgccgg gggtgttcct gctgaagaac ctggcggacg
      781 ccatccggat caaggcgcat ctgaaccgga gctcctgccg caaagccgtg gtcatcggcg
      841 cggggttcat tggcatggag atgtgcgagg ccctccgcga ccgcgggatg gagacgacgg
      901 tcgtccatcg cggcgacttg ccggtcgcgc gctgggacgc cgaattcgcc cgggagatcg
      961 tcgccgccct ggagcggaac ggcgtggcct ttctcgcgga tacggagctg cacgggatcg
     1021 aggcgggaac gtcctgtccg ctcaaggtga tgacgagccg gggaccgatc gaggcggacc
     1081 tggtcctcgt ggccgtcggc atcgccccga acgtgttgtt ggcccgggat atggggctcc
     1141 ggatcggcgc gacgggtgcg atcggcgtcg acgcgcaaca gcagacatcg gccccgaacg
     1201 tttacgccgt tggcgattgc tgcgaggtgt accaccgggt gagcagccgg tgggcctatc
     1261 tgcccctggg tgacacggcc aacaagcagg gccgcctggc gggacggatc atcggcggcc
     1321 gccccgccgc gcacccgggc gtcgtcgggg cccagtcctt caagatcttc gagctcgaaa
     1381 cggcggcaac gggcctgaac gaggcggagg cgctgcaggc ggggttcgat cccatcggcg
     1441 acgtctactg gggcaacgcg atcgccggtt actttgggaa gcggaaactc ggcctgaaac
     1501 ttttggcgga ccgggcgtcc ggcaaacttc tgggcgcaca ggccatcggg gaaacggggg
     1561 cggtcggtcg catcaacact ctttcggcgg cgttgtggtc cgaattgacc ctggacgaga
     1621 tcggctggct cgatctcgcg tacgccccgc ccttcggcgg ggcctgggac gtgatccatc
     1681 gcgccgccca gagattgcag aggaagcgtt aaacaatgtc ggaacagatg gtgtattggg
     1741 gaattttcat cgcctttgtg atcgtcatgc tcgtcctgga cctgggcgtc ttcaaccgta
     1801 aggcccatgt aatcaaggtt cgggaagccc tgatgtggac ggggttctgg gtcagcctcg
     1861 ccctgctgtt ctgcctgggg gtttattggg tcaaggggtc ggagaaggcc ctggccttcc
     1921 tcgccggcta cctcatcgag gaatccctga gcgtcgacaa cctcttcgtc tttctcatga
     1981 tcttttcgta cttcgggatc ccgccgcagt acgagcacaa ggccctgttc tggggcatca
     2041 tcggggcgct cgtgatgcgg gcggtgttca tcgcggccgg cgtggccctg atcgagcagt
     2101 tccactggat catctatgtc ttcggtgcct tcctcatttt caccgccgtc aagatggcgc
     2161 ggagcgggga agggaatgtc cacccggacc ggaacgtcgc cctcaagctc ctgcgcaagg
     2221 tcatgccggt gacgaacgat tacgccgggg ggaaattttt cgttcgggaa ggggggcggt
     2281 ggatggcgac gccgctgttc gccgtggtcc tcgtcctgga gacgacggac gtgatcttcg
     2341 ccgtggattc catcccggcg gtcctcgcga tcacgaccga tcccttcatc gtctatacct
     2401 ccaacgtgtt cgccatcctg gggctgaggt cgatcttctt cgccctggcg gggatgatgc
     2461 gcctgttcta ctatctgcgc tacggcttgg tggtggtcct gtcctttgtc gggatgaaga
     2521 tgatcctgac ggagtgggtc aagatcccgg tgggcctttc ccttggcgtc atcgcggcgg
     2581 tactggcggt gtcgatcgtc gcttcgctgg ccctgccgca ccccgtccgg atggtgatcg
     2641 acgacgaggc cgggaagaag gagaatccgg tcggatgatc cccggcgggt gtccgggtag
     2701 gggaagcccg gtgttgtaaa ggccgatcat gccgtctcca tcctcgaggg cgtacacctg
     2761 cgaatgcagg gatgtcaagc cggttccggc cgaatcacgg caggccctcc tcgtgaccgc
     2821 gccgggctgg aacgccgttg ccgccaccct cgagcggtat gaacggacgg gtgcgggggt
     2881 accgtggctg gccgtcgggg agcggatccc cgccgtcctg gggcgcaacg gtttggcttg
     2941 ggggcggggc ctggcgactc ccccactgga cggtccggtc aaagaagagg gggacggccg
     3001 gtctcccgcg ggcgtgttca ggatccgcgg tgccttcggc tatgccccgg cggcggatgt
     3061 cccctggatc cgtctgccgt accgtcaggc cacggccggg ctgaaatgcg tggacgatca
     3121 cgcttcggcc cgctacaacc gcctcgttga caccgccgtg gagccacaga cctggagccg
     3181 ccacgaggag atgctgagga aggacgatct ttaccgcctc ggggccgtga tcggccacaa
     3241 cgacgatccg accgtgtccg gcggcggctc ctgcatcttc ctccatgcct ggggcggacc
     3301 ggaccggggc acggcggggt gcaccgccgt ggcgctgccg cggctcgaag acatcctcca
     3361 ctggctcgat ccggccctgt gtccggttct cgtgcagctg gtggtggagg accgggagcg
     3421 gctgcgcacg gcttgggacc ttccgtaacc ggagggaaca gaagggaccg ggtccgtgcg
     3481 ggatgccggg ttgttccacc cgccggcgcg ggcggggccg ggcatggccg ttttctgaag
     3541 gatgcgtcga ttcccccggg ggttcatgat ttgcaggcat tatcgtccgc gcgcgtgacc
     3601 gcatgggcca agctgaacca gacgaaatac cgccggcggg agaggctgtg cctcgccgag
     3661 ggggagaagg tcgttcggga actcctgcgg agtccgtggg cggttcaggc cgtgctggtg
     3721 gcggaggacg ccgcggcgcg ctaccaggcc ctggtcgcgt ccctgccgtc ggggtcggtc
     3781 catgcggttc cggcacggtt ctggaagcgc cttagccagg accccgcccc ggagggcatc
     3841 atggccgtgg ccgccctgcc gccgcccctt gccgcggccg cccgggcccc gggccccggg
     3901 ccgctcctcc tgatcgaccg ggtcaacaat cccaacaatc tcggggcgct cctgcggacg
     3961 gcccactggt ttggcttccg gacggtgctc gtcggcgccg ggtcggcgga ctggacccac
     4021 ccgaaggtca tccggacgtc catgggcagc ctctttcacc tgaacgtgac agccgatctc
     4081 gatctttcgg cggtgctgcc ggcactccgg gggcgtttcc gcctcatcgg cagtgatgtc
     4141 cgccggggcg tcccgcccca tccctgcccg ccgcaaacgg ccctgctgct gggcaacgag
     4201 acccacggtc tgccgccggc gctgctcgac ctgacggacg aatgctggtg catccccggc
     4261 gccggcgccg ccgagtctct gagcctgccc caggccgcag cggtcatgat gtacgaattc
     4321 aggaaggggg aatagccatg cagtgggttt ttcttgccgt cgcgattctc gcggaggtga
     4381 ttggcacggc ggcgctcaag gtttccgacg gattcaccca ccccgttcca tcgctggtcg
     4441 tccttctggg gtatggaatt tctttcttct tcctctccct gacgctgcgg accctgccgg
     4501 tcggcgtggc ctatgcggtg tggtccgggg ccgggatggc cctcatcgcc ctgatcggct
     4561 ggcttttcct ggggcagtct ctggatgcgc ccgccatcgt cggtttgttc ctgatcggcg
     4621 ccggcgtggc ggtgctgaat ctctattccc ggtccgtcgt gaattgaacg ggccgcgggt
     4681 tggatcgcgt tcggatcgac cgggcggctg gccgggtctc cggagatccc gaaaaggagg
     4741 cgacatgctg ctggcgggcg acat
//
LOCUS       DAOOLJ010000099         4755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_21620_length_4755_cov_20.984043, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000099 DAOOLJ010000000
VERSION     DAOOLJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21620_length_4755_cov_20.984043"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..902)
                     /gene="era"
                     /locus_tag="PLW16_09925"
     CDS             complement(12..902)
                     /gene="era"
                     /locus_tag="PLW16_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPB71266.1"
                     /translation="MFKSGFIGIIGRPNVGKSTLLNAVVGERIAITTPKPQTTRNRIT
                     GIRNVPNGQMIFLDTPGIHRADTPLNQLMVGAATDAFRSADLLLLLVEAPAGVHRGDL
                     SILRGLEKVRRPVILVINKTDLVPADALPALTAAFREAFPFADALALSALKERGIDAL
                     LDTLWQLLPEGPPFFPEDQMTDRSERFVAAEIVREQVILLTHKEIPYSTAVVVDTFRE
                     DEAKKLISIQATIHVERDSQKGILIGKGGGMLKRIGTAARLEMEKFFAARIYLELFVR
                     VQKDWTHDKRMLREFGYSEK"
     gene            1174..3135
                     /locus_tag="PLW16_09930"
     CDS             1174..3135
                     /locus_tag="PLW16_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPB71267.1"
                     /translation="MRTVAITALCTALITLFTGLFPAAPAAWAASPETALMAAHRAYL
                     RGDRAALARQAEAVRGHALEAYADFWRLKLGLGEADPADVRDFLARNAGTVLAEQLRR
                     DWLRILGRTGQWALFRQERPLLAQSDADITCYALQDRWRRREGPVRPGLDPFWNASRP
                     LPAACEPVADALYESGALTPDDLRDRFRLLVRNNRLSAAERIAARLPEDRAPRPDRIQ
                     AVSRAPAQFLEGPEVDLRTAASRELAIVALSRLAADDPTAAAERWSRMAQAAFPIEDQ
                     QYVWAVLATHGAYRRVPEAVDWFRRAGGTPLTDEQLAWRARIALRQHRWAEVNAAVER
                     MSPASRREAAWTYWQGRALVALGRPEEGRKLYARLAGGTDFYGLLAAEELGRSLAVPP
                     PAAPPTAEELAAAAGKPGLKRALLLYRLGLRSEATLEWQWAVRSLDDRSLLVASELAC
                     RNGLWDRAIVTANRTVAAHDFSLRYPTPYRDVLSGQARLRRLDEALVFGLVRQESRFI
                     AHALSSAGARGLMQLMPATARRVARKIGLKDYRLSRIHQPEVNTALGTGYLRSMLDQF
                     DGSPVLAAAAYNAGPGRAHRWRDHAGPLEGAIYVETIPFSETRRYVKKVMTNAMYYAA
                     LLGGGAQLPLKSRLGTVAGTAGSRPPEDE"
     gene            3225..4115
                     /locus_tag="PLW16_09935"
     CDS             3225..4115
                     /locus_tag="PLW16_09935"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB71268.1"
                     /translation="MFRLRVILLCVVFSLGLCPAGYAAQSAITEADGYSCMGVDKSRK
                     QTEQEALADAKRNAVENAKTHVKSQTTVKDFQIEEDLIDAYAQASVRIVRELERGWYR
                     DAVSGDCFKIRIQAEVIPEEGAMSRIGREKGGFPDPAAPLNVKIWTDRKAYRQGERIK
                     IYLQGNKPFYARILYKDAGGNLLQLLPNAHRRENYFNGGVIYELPAGDDRFELEVSPP
                     FGMENVLIYAGTAPLGDLDLREMGAAAGGVYQVQTEGAEVGLKTRGIKVTGKGAGAAP
                     NVPATAEFVESSVGLTTGGR"
     gene            complement(4232..4705)
                     /locus_tag="PLW16_09940"
     CDS             complement(4232..4705)
                     /locus_tag="PLW16_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPB71269.1"
                     /translation="MNGWSDRKSRRARRIGVAAVCILCVLFTGCLGWILEKPTIVVRE
                     ISLTPRSLLEMELRLGIEVHNPNRFDLTLTALDYTLFLDDRKIGEGRLEQALRIPAAS
                     TTSIRAPLSVRFGDLGGGVRALLTGGEIPYRIEGSAVIKTVFGSDTFPFTKDGHL"
BASE COUNT          738 a         1622 c         1659 g          736 t
ORIGIN      
        1 tccggcccgc cttacttctc gctgtacccg aattcccgta gcatgcgctt gtcgtgggtc
       61 cagtccttct ggacccggac gaagagctcc aggtaaatcc tcgcggcgaa gaatttttcc
      121 atctccaggc gcgccgccgt gccgatccgc ttcagcattc ccccgccctt gccgatgagg
      181 atgcccttct gcgagtcccg ctccacatgg atcgtcgcct ggatggagat cagtttcttc
      241 gcctcgtcct cccggaaggt gtccacgacg acggccgtgc tgtaggggat ttccttgtgc
      301 gtcagcagga tgacctgctc ccggacgatc tcggcggcca cgaagcgctc ggagcggtcc
      361 gtcatctggt cctcggggaa aaagggcggc ccctcgggga ggagctgcca gagcgtgtcc
      421 aggagggcgt cgatgccccg ctccttcagc gcggacaggg cgagcgcgtc ggcgaagggg
      481 aaggcctccc ggaaggcggc cgtcagggcg gggagggcgt ccgccggaac caggtcggtc
      541 ttgttgataa cgaggatgac cggccggcgg accttttcca gcccgcggag gatggacagg
      601 tccccccggt gcacgccggc gggggcctcg acgagcagga gcagcaggtc ggccgagcgg
      661 aaggcgtcgg tggcggcgcc gaccatgagc tggttcaggg gcgtgtcggc ccggtggatc
      721 cccggcgtgt cgagaaagat catctgaccg ttcgggacgt tccggatgcc cgtgatccgg
      781 ttgcgcgtcg tctggggctt cggcgtggtg atggcgatcc gctcgcccac gacggcgttc
      841 agcagggtcg atttgccgac gttggggcgg ccgatgatgc cgatgaatcc ggatttgaac
      901 aaggcgattt tccttcccgg ggacgggtgc gccccctgcc ggatccggac cgcggggatc
      961 gcgatccggt ttttccggtt ggagatgttt ctatagcgca gccgtcgggg gtctgtctaa
     1021 tgaaatcgct tcgcccggcg tccccggagg cgtcgcgtct cccgggaacg tgccgaaaac
     1081 ggcgcgcccc gggttttgac tgcaaagaaa gaggcaattt tgagcggaac tccgacgaaa
     1141 cttgtgatac aagggcgcgc ggaggtcaac ggaatgcgga cagttgcgat cacggccctg
     1201 tgcacggccc tcatcacgct ctttaccggc cttttcccgg ccgcgcccgc ggcgtgggcc
     1261 gcgtcccccg agacggcgct gatggccgcc caccgcgcct atctccgggg cgaccgggcg
     1321 gccctcgccc gccaggcgga ggccgtccgc gggcacgccc tcgaggccta cgcggatttc
     1381 tggcgtctca agctgggact gggcgaggcc gacccggcgg acgtgcggga ctttctcgcc
     1441 cgcaacgccg ggaccgtcct ggccgagcaa ctgcgccgcg actggctgcg catcctgggg
     1501 cgcaccggtc aatgggcgct cttccgccaa gagaggccgc tgctggccca aagcgatgcc
     1561 gacatcacct gctatgcgct tcaggaccgt tggcgccggc gggaaggccc ggtccggccg
     1621 gggctagacc ccttctggaa cgcctcccgc cccctgcccg ccgcctgcga accggtcgcc
     1681 gacgcgcttt acgaatccgg cgcgctcacc ccggacgacc tccgggaccg attccggctc
     1741 ctcgtccgca acaaccggct gtcggcggcg gaacggatcg cggcgcgtct gcccgaagac
     1801 cgggcacccc ggccggaccg gatccaggcg gtttcccggg ccccggccca gtttctggag
     1861 ggtccggagg tggatctgcg gacggcggcc agccgcgagc tggcgatcgt cgccctgtcg
     1921 cgcctggccg cggacgaccc gacggcggcc gcggagcgct ggagccggat ggcgcaggcg
     1981 gcgtttccga tcgaggacca gcagtacgtc tgggcggtgc tggcgaccca cggcgcctac
     2041 cggcgcgtcc cggaggccgt ggactggttc agaagggctg gcgggacgcc gctcacggac
     2101 gaacagttgg cctggcgcgc gcgcatcgcg ctcaggcagc accgctgggc ggaggtcaac
     2161 gccgccgtcg agcggatgtc ccccgcttcc cgccgcgaag cggcctggac ctactggcag
     2221 gggcgcgcgc tcgtcgcgct cggccggccg gaagaggggc ggaagctgta cgcccggctc
     2281 gccggcggga cggatttcta cgggcttttg gcggcggagg agctgggacg gtcgctcgcg
     2341 gtcccgccgc ccgccgcgcc gccgaccgcg gaggagcttg ccgcggccgc cgggaagccc
     2401 ggtctgaagc gggcgctgct gctctaccgc ctcggtctga ggtccgaggc cacgctggag
     2461 tggcaatggg ccgtccgttc cctggacgac cgctcgctgc tggtggcctc ggaactggcg
     2521 tgccggaacg gcctctggga ccgcgccatc gtcacggcga accggaccgt cgccgcgcac
     2581 gacttttcgc tccgttaccc gacgccctac cgcgacgtcc tgtccgggca ggcgcgcctg
     2641 cgccggctcg acgaggccct ggtcttcggc ctggtgcgcc aggaaagccg cttcatcgcc
     2701 catgccctgt cctcggcggg cgcccggggc ctgatgcaac tcatgcccgc cacggcccgc
     2761 cgggtcgccc ggaaaatcgg cctgaaggac taccgcctgt cgcgcatcca ccagccggag
     2821 gtcaacaccg ccctggggac cggttacctc cggagtatgc tcgaccagtt cgacggcagc
     2881 ccggtgctcg ccgccgcggc ctacaacgcc gggccgggcc gggcccaccg gtggcgcgac
     2941 cacgccgggc cgctcgaggg cgcgatctac gtcgagacga tcccgttttc cgaaacccgc
     3001 cggtacgtca agaaggtgat gacgaacgcc atgtactacg ccgccctcct cggcggcggg
     3061 gcgcagctcc cgctgaagtc ccgcctgggg accgttgcgg gaacggccgg aagccgcccg
     3121 ccggaagacg agtgagcgcc ccggcccggc aaaactcttc gcttgacgcc ggcgcgccgt
     3181 tcggggatga taggggccga cgaaagacga aggagggaag gggtatgttc cgattgcgcg
     3241 tcatcctgct gtgcgtggtt ttttctctgg ggctctgccc cgccgggtat gccgcccagt
     3301 ccgccatcac cgaggcggac ggctactcct gcatgggggt cgataaatcg cggaagcaga
     3361 cggagcagga ggccctggcg gacgcgaagc gcaacgccgt cgagaacgcc aagacccacg
     3421 tgaagagcca gacgaccgtc aaggacttcc agatcgagga ggaccttatc gacgcctacg
     3481 cccaggcctc ggtccggatc gtccgtgagt tggaacgggg ctggtaccgg gatgccgttt
     3541 cgggagactg cttcaagatc cggatccagg ccgaggtcat ccccgaggaa ggggcgatgt
     3601 ccaggatcgg ccgggagaag ggcgggttcc cggatccggc cgcccccctg aacgtgaaga
     3661 tctggacgga ccggaaggcc taccgccagg gggaacggat caagatctac ctccagggga
     3721 acaagccctt ctacgcgcgg attctttaca aggacgccgg gggaaacctg ctccagctcc
     3781 tgccgaacgc gcatcgtcgc gaaaattact tcaacggcgg cgtgatctac gagctgcccg
     3841 ccggggacga ccgtttcgaa ctcgaggtgt ctcccccctt tggaatggag aatgtcttga
     3901 tctatgccgg caccgccccg ctgggcgacc ttgacctcag ggagatgggg gccgccgccg
     3961 gcggcgtcta ccaggttcaa acggaagggg cggaagtcgg cttgaagacc cggggcatca
     4021 aggtcacggg aaagggtgcg ggcgccgccc cgaacgtccc cgcgaccgcg gagttcgtcg
     4081 agtcgtcggt cggcctcacg acgggcggcc gctagcccga cgcgacggaa gaacgcgcat
     4141 cggcgtcgat gaatgcagaa agcggcggcg gggttcccaa cgggccatcc gcgcggaacg
     4201 agcgtccgac cccacactcc cgctccgctt cctaaagatg cccatccttc gtgaagggga
     4261 acgtgtcgct cccgaagacc gtcttgatga cggcgctgcc ctcgatccgg tagggaatct
     4321 cgccgccggt caggagcgcc cgcaccccgc cgcccagatc cccgaaacgg acgctgaggg
     4381 gcgcccggat cgacgtcgtc gatgcggcgg ggatccggag cgcctgctcg aggcggcctt
     4441 cgccgatttt ccggtcatcg aggaagaggg tatagtccag ggcggtcagc gtcaggtcga
     4501 accggttggg gttgtggacc tcgatgccca gccgaagctc catctccagc agggagcggg
     4561 gcgtaagcga gatctcccgg acgacgatgg tgggcttttc caggatccag cccaggcacc
     4621 cggtgaacaa cacgcacagg atgcagacgg ccgcgacgcc gatgcgcctc gccctccgcg
     4681 attttcgatc gctccaaccg ttcatcacag ccccctcttt gccggatcgt ctccgggcgt
     4741 tcgtttcatc acacc
//
LOCUS       DAOOLJ010000100         4709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_21883_length_4709_cov_14.880748, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000100 DAOOLJ010000000
VERSION     DAOOLJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21883_length_4709_cov_14.880748"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="hisS"
                     /locus_tag="PLW16_09945"
     CDS             <1..1046
                     /gene="hisS"
                     /locus_tag="PLW16_09945"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPB71270.1"
                     /translation="RPEATASILRAYLEHTLYAADPLARLFTIGPMFRRERPQKGRLR
                     QFHQLDAEILGIDDPRVDAELIVMLMHFLVRAGLPDIHLEINSLGCPDCRPTFRDRLI
                     TFLAGKQTALCEDCRRRMHSNPLRIFDCKLESCREQLQSAPKILDSLCSPCNAHFQTF
                     REYLSIADITPLINAGMVRGLDYYTRTAFEVTTGALGAQNAVLGGGRYNGLARELGGP
                     DIPGIGFAIGMERLLSLIEPNGAQLSPAPRLFIAILGDQAGRIAFDLANGLRKVGMRT
                     EIDYAGRSLKSQMRRANKLNCPYTLILGDRELASGEVQWRDMRGSLQETLPLNGLFET
                     VRRKLLEKGQTLV"
     gene            1021..2817
                     /gene="aspS"
                     /locus_tag="PLW16_09950"
     CDS             1021..2817
                     /gene="aspS"
                     /locus_tag="PLW16_09950"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPB71271.1"
                     /translation="MRKVKHLSEFITQIKRTHYCGTLTAADDGRTVVLMGWTHRRRDH
                     GGVIFVDLRDREGLVQIVFNPEISPDIHREAHRIRSEYVIAVRGQVRTRPEGMQNPDL
                     KTGDVEVMVHDLQILNEAKTPPFLLDSEAEISENVRLKYRYLDLRRPVIQKNLLLRSM
                     AASETRAYLIDNGFIEVETPFLTKSTPEGARDYLVPSRVNPGAFYALPQSPQIFKQLL
                     MVAGFDRYFQIVKCFRDEDLRADRQPEFTQIDCELSFISEEDVLRIMEGLMIHLFRSV
                     LGIELDRPFPRLSFHEAMDRYGKDAPDVRFGLELTDLTDLLSDSAFVIFQEAFATGGV
                     VKAIRVDGAQKLSRKDIDGLNDFITALGAKGLAWARVTPDGWTSPIVKFMTPTEIADI
                     NRRMQAAEGDLLLMMADAPGLVRDILGNLRLHLARKLDLVRPDQLAFTWVTEFPLFEY
                     SESEKRYVSTHHPFTSPVDEDIPLLLTDPGKVRARAYDLVLNGSEIGGGSIRIHRQEV
                     QSLIFQALGLPADEIRQKFGFLLDALEYGTPPHGGIAFGLDRLMMIMTGAESIRDVIA
                     FPKTQKATCLLTDAPGPVSIEQLMELSLKIVS"
     gene            2881..>4709
                     /locus_tag="PLW16_09955"
     CDS             2881..>4709
                     /locus_tag="PLW16_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HPB71272.1"
                     /translation="MPKWNQERKVLDHEHTRFWRYDLKDIDEPNLQKDVFPYEEISRI
                     DFDHKLIPITPADDLFITDTTFRDGQQARPPYTVRQIVDLFKLMSRLGGERGIIRQSE
                     FFLYSQKDKEAVAQCVALNLPYPEVTGWIRASREDIDLVKNSGLKETGILTSVSDYHI
                     FLKLNMNRRKALDEYLGIVKSILDEGIVPRCHFEDITRADIYGFCIPFALELMKLREE
                     SGIDIRIRLCDTMGYGVTYPGASLPRAIDKMVRAFVDDAGVPGHLLEWHGHNDFHKAL
                     INASTAWLYGCSAANGTLLGLGERAGNPPIEGLIIEYIGLRGRPDGIDTTVITDIAHY
                     FDREIGYHIPANYPFVGADFNVTRAGVHADGLIKCEEIYNIFDTSRLLKRPIVPMITD
                     KSGKAGIAYWINSHLHLAEESQVDKRHPGISKIHKWIVDQYEGGRMTSISNEEMEKLI
                     RKYIPELFMSELDKIKYRAAEAAVAIVKQVIEHPVMKSMQPELQEPVMLQCIEENPSI
                     QFAYVVDMNGKKTTRNITNIADRVKYENYGVGTDQSDREWFIRPLQTGKMHVTNFYIS
                     KMTGALCITVSAPIVDEKDEMVGIFGVDIKFEDWVKRVEDIAEA"
BASE COUNT          962 a         1510 c         1344 g          893 t
ORIGIN      
        1 tgaggccgga agcgaccgcg tcgattctcc gcgcctatct cgagcacacc ctctacgccg
       61 ccgatccgct ggcccgcctc ttcaccatcg ggcccatgtt ccggcgggaa cgcccccaga
      121 agggtcgcct tcgccagttt catcaattgg acgcggagat tctcgggatc gacgatcccc
      181 gcgtggacgc ggagctgatc gtcatgctca tgcattttct cgtccgcgcc ggcctgccgg
      241 acattcatct cgaaatcaat tccctgggat gtccggactg ccgccctacg ttccgagacc
      301 ggctcatcac ttttctcgcc gggaaacaaa cggccctgtg cgaggactgc cgtcgcagga
      361 tgcactcgaa cccgctccgg atcttcgatt gtaaactcga gagctgccgc gagcagcttc
      421 agtcggcgcc gaagatcctc gactcccttt gttccccgtg caacgcccat ttccagacat
      481 tcagggaata cttgagcatt gcggatataa cgcctttgat caacgctgga atggttcgag
      541 gtctcgatta ttatacgcgt acggccttcg aggtcacgac gggggctctc ggcgcccaga
      601 atgccgtcct cggcggcggc cggtacaacg gtctcgcacg cgaactcggc ggccccgaca
      661 tcccgggaat cggcttcgcc atcggcatgg agcggctcct gtccctgatc gagccgaacg
      721 gtgcgcaact cagccccgca ccgcgcttgt ttatcgccat tttgggagat caggccgggc
      781 gtattgcgtt cgatctcgcc aacggtctgc ggaaagtcgg catgcgcacg gagatcgatt
      841 acgccgggcg cagcctcaaa agccagatgc ggcgcgctaa caagctgaac tgcccttaca
      901 ccctgatcct gggcgatcgc gaactcgcct caggcgaggt gcagtggcgc gacatgcggg
      961 gcagtcttca ggaaaccctt cccctgaacg gccttttcga gaccgtccgg agaaaattgc
     1021 ttgagaaagg tcaaacactt gtctgaattc atcacccaaa tcaaaaggac ccattactgt
     1081 gggacgctga cggccgcgga cgacgggcgt accgtcgtac tgatgggatg gacgcaccgg
     1141 cggcgggatc acggcggggt catcttcgtt gatcttcgcg accgcgaagg acttgtccag
     1201 atcgtcttca atccggagat cagtccggac atccaccggg aggcgcaccg catccggagc
     1261 gaatacgtga tcgccgtccg gggtcaggtc cggacccgcc cggaaggcat gcaaaacccc
     1321 gacctcaaaa ccggagacgt cgaggtgatg gtccacgacc tccagatcct gaacgaggcg
     1381 aagacgcctc cgtttctcct cgattccgag gcggagattt ccgaaaacgt ccgcctgaaa
     1441 taccgctacc tggacctgag acgtcccgtc atccagaaga atctcctgct ccggagcatg
     1501 gccgcgtcgg aaacccgggc ctatctgatc gataacggct tcatcgaggt agaaacgcct
     1561 tttctcacga agagcacacc cgaaggggcc cgcgattacc tggttccgag tcgcgtcaac
     1621 cccggcgcct tttacgcgct gccccagtcc ccccagatct tcaagcagct cctgatggtg
     1681 gccggcttcg accgctactt tcagatcgtc aaatgcttcc gggacgagga cctgcgcgcg
     1741 gatcgccaac ccgagttcac ccagatcgac tgcgagctgt ccttcatctc cgaggaggat
     1801 gtcctccgga tcatggaggg gttgatgatc catctcttcc gctccgtgct cggcatcgag
     1861 ctcgaccggc cgtttccccg cttgtccttt cacgaggcga tggaccgcta cgggaaggac
     1921 gcccccgatg tccgcttcgg cctcgaactc acggacctca ccgacctgct gtcggacagc
     1981 gctttcgtga tcttccagga ggcctttgcc accggcggcg tcgtcaaggc gatccgggtc
     2041 gatggcgccc agaagctttc ccggaaggac atcgacgggt tgaacgattt catcaccgcc
     2101 ctgggcgcga aggggctcgc ctgggccagg gtcactccgg acggctggac ctccccgatc
     2161 gtcaaattca tgacgccgac cgaaatcgcg gacatcaatc gtcggatgca ggcggccgaa
     2221 ggcgacctgc tcctcatgat ggcggatgcg cccggtctcg tccgcgacat tctcgggaac
     2281 ctccgcctcc atctcgctcg gaaactcgat ctcgtccgcc cggatcaact ggccttcacg
     2341 tgggtcacgg aatttccgct cttcgagtac agcgagtcgg aaaagcgcta cgtctccacg
     2401 caccatccct tcacctcacc ggtggacgag gatatccccc tgttgctgac cgatcccggc
     2461 aaagttcggg cgcgtgccta cgacctcgtc ctgaacgggt cggaaatcgg tggcgggagc
     2521 atccgcatcc accgccagga ggtgcagtcg ctgatcttcc aggcgctcgg ccttcccgca
     2581 gacgagatcc gccagaagtt cggcttcctc ctggacgccc tggagtacgg cacaccgccg
     2641 cacggcggta tcgccttcgg tctggaccgc ctgatgatga tcatgacggg cgcggagtcg
     2701 atccgcgacg tcatcgcctt cccgaaaacg cagaaggcga cctgcctcct caccgacgcc
     2761 cccgggccgg tgagcatcga gcagctgatg gagctttccc tgaagatcgt ctcctgacgc
     2821 ctttttctgt tgacaaagac acgctcgctt tataccttca gcgggtcagg aaaatgaacc
     2881 atgcctaaat ggaatcagga gaggaaggtt ctcgatcacg aacatacgcg gttctggcgt
     2941 tacgatctga aagacatcga cgagccgaat cttcagaagg acgtctttcc gtacgaggag
     3001 atctcccgga tcgacttcga ccacaagctc atcccgatca ccccggcgga cgacctgttc
     3061 atcaccgaca cgacgtttcg tgacggtcag caggcgcgtc cgccctatac ggtgcggcag
     3121 atcgtcgacc tgttcaagct gatgagccgc ctgggcgggg agcgggggat catccgccag
     3181 tccgagttct tcctctacag ccagaaggac aaagaggccg ttgcccagtg cgtcgcgctg
     3241 aacctgccct accctgaggt cacgggctgg atccgggcca gccgggagga catcgatctg
     3301 gtcaagaatt ccggtctgaa ggaaaccggc atcctgacct cggtgtccga ctatcacatc
     3361 ttcctcaaac tcaacatgaa ccgcaggaag gccctcgacg aatacctggg cattgtaaag
     3421 tcgatcctgg acgaaggcat cgtcccgcga tgtcatttcg aggacatcac gcgggccgac
     3481 atctacggat tttgcatccc gttcgccttg gagctgatga agcttcgcga ggagagcggc
     3541 atcgacatca ggattcgcct ctgcgacacg atgggctacg gtgtcacgta tcccggggcc
     3601 tccctgcccc gcgcgatcga caagatggtg cgcgccttcg tcgacgatgc cggcgttccg
     3661 ggtcatcttt tagagtggca cggccacaat gactttcaca aggccctgat caatgcctcc
     3721 accgcctggc tgtacggctg ctccgccgcc aacggcaccc tgctgggcct gggcgaacgc
     3781 gccgggaacc ctccgattga aggcctcatc atcgagtata tcgggctccg cggccgaccg
     3841 gacggcatcg acaccacggt catcaccgac atcgcccact atttcgacag ggagatcggc
     3901 tatcacattc cggcaaacta tcccttcgtc ggggccgact tcaacgtgac ccgggccggc
     3961 gtccatgccg acggactcat caagtgcgag gagatctaca acatcttcga caccagccgc
     4021 ctactcaagc ggccgatcgt gccgatgatc accgataaat ccggcaaggc cggcatcgcc
     4081 tactggatca actcccacct tcatcttgcc gaggagagcc aggtcgacaa gcgtcacccc
     4141 ggcatctcca aaatccacaa gtggatcgtc gaccagtacg agggggggcg gatgaccagt
     4201 atctccaacg aggagatgga gaagctcatc cgcaagtaca taccggaact gttcatgtcg
     4261 gaactggaca agatcaaata ccgggcagcg gaggcggcgg ttgcgatcgt taaacaggtc
     4321 atcgagcacc cggtgatgaa gtccatgcag cctgaacttc aggaacccgt gatgcttcag
     4381 tgcatcgagg agaacccctc gattcagttc gcctatgtgg tcgatatgaa cgggaagaag
     4441 acgacccgaa acatcacgaa catcgccgat cgggtcaagt acgagaatta cggtgtcggg
     4501 acggaccagt cggaccggga gtggtttatc cggccgctac agaccggtaa aatgcacgtg
     4561 acgaacttct acatctccaa gatgacgggt gccctgtgca tcaccgtttc cgcgccgatt
     4621 gtcgacgaga aggatgagat ggtcggcatt ttcggcgtgg acatcaagtt cgaagactgg
     4681 gtcaagcggg tcgaagacat cgcggaagc
//
LOCUS       DAOOLJ010000101         4689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_22010_length_4689_cov_17.924040, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000101 DAOOLJ010000000
VERSION     DAOOLJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22010_length_4689_cov_17.924040"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLW16_09960"
     CDS             <1..297
                     /locus_tag="PLW16_09960"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPB71273.1"
                     /translation="NSWMEPLKKRFDIKILGQELSVSSDSGDEHVAKVVGYVTERVEE
                     IGRTAGKATTLNVAILTALNIADEYFKLKGENERIVKQLEARSENLIHLINELN"
     gene            305..496
                     /gene="ssrS"
                     /locus_tag="PLW16_09965"
     ncRNA           305..496
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLW16_09965"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            703..2265
                     /gene="rny"
                     /locus_tag="PLW16_09970"
     CDS             703..2265
                     /gene="rny"
                     /locus_tag="PLW16_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPB71274.1"
                     /translation="MSSGLIILFIVLAGAVGLVLGYALKQRLSRKQRGASERLGARIV
                     DEAKKEAENIKKEAILQAKENLLKAKTEFERDAQERKTELDNLEKRLRTKEEAIDKRI
                     DTIVQKEANLEGRDKALTQKESQIGEKHGRLDRMIEEQREQLEKAAGISAEEAKALLV
                     QAMETEAKHEAALAIRKIEDETRRTADKKARDILAYSIQRYAGDVVAENTVSVVSLPN
                     DEMKGRIIGREGRNIRAIEAATGIDLIVDDTPEAVVLSSFDPVRREVARLSLERLITD
                     GRIHPGRIEDVVKKVATEVDSIIRETGERTSFDVGVHDIHPEILKLLGSLKYRTSYSQ
                     NVLQHSVDVAHLTGMMASELKMNVKEAKRAGLLHDIGKAIDHKVEGPHAAIGADFAKR
                     YGESPRIIQAIATHHDDGRTNTILGVLVQAADTLSAARPGARREMLETYVRRLEELEA
                     IANSFTGVDKCFAIQAGREIRILVENEKISDNDAVMLCRDIVKKIEAELTYPGQIKVT
                     VIRETRVSEFAK"
     gene            2332..3120
                     /locus_tag="PLW16_09975"
     CDS             2332..3120
                     /locus_tag="PLW16_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPB71275.1"
                     /translation="MNILFIGDIIGKPGRRAVRELLPRLVDEARIDFVIANCENAAAG
                     FGVTRDVVEELYRDGVHVLTSGNHFWDKKEVLEFAEDYETLLRPANYPDGVPGWGSAI
                     LPTSTGEYVGVLNLAGRVFMAPLDCPFRTADRELARLEKKARIIIVDVHAEATSEKQA
                     LGWYLAGRVSAVIGTHTHVQTADACIRPGGTAYLTDAGMTGPFDSVIGIGKDIILERF
                     LSLTPRKYDVAKGDIRLQGAVVTVDEKTGRATDIRAISRKLETG"
     gene            3141..4421
                     /gene="tyrS"
                     /locus_tag="PLW16_09980"
     CDS             3141..4421
                     /gene="tyrS"
                     /locus_tag="PLW16_09980"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB71276.1"
                     /translation="MSVFDVFRERGFIEQVTDEALVEDLLAREKVTCYIGFDPTAASL
                     HVGSLVPIMALAHMQRHGHRPIALIGGGTALIGDPSGKTEMRRILTRAQVDHNAACIR
                     EQLSQYLDFSDGKALLLNNADWLARLNYIEFLRDIGRHFSVNKMLAAESYRIRLEKGL
                     NFIEFNYMLLQAYDFLHLFKQEGCVLQMGGNDQWGNMLAGSDLIRRLEGKNAFSLTFP
                     LMTTSAGHKMGKTEKGTVWLDRSLTSPYDYYQYWINAEDADVERFLLLFTFLPVEEVR
                     AVRALSDARLNMAKAVLAFEATKVTHGEAAAADAWRASCQAFGTKPVAEGLFPSSTIP
                     RRDAAQDVSAIPCLVKSRDDMGAGIPAFELFHEAGLCASRGEARRLIQQGGGYVNERP
                     VDRIDEIIGLGHCDAQGEIRLRKGKKTYFIVRVK"
BASE COUNT          991 a         1339 c         1526 g          833 t
ORIGIN      
        1 aactcttgga tggagccttt gaaaaagcgc ttcgacatca agatactggg gcaggagttg
       61 tccgtatcga gcgattcagg ggatgaacat gtagcaaaag tcgtcgggta tgtcacggag
      121 agggtggaag agatcggccg gacggccggc aaggcgacca ccctgaacgt ggccatcctg
      181 acggcgctca acattgcaga tgagtatttt aaacttaagg gagaaaacga gagaattgtc
      241 aagcagttgg aagcaagatc ggaaaattta attcatctga tcaatgaatt aaattgatcc
      301 ctgaaatccc ctgcgatgtg cgtgattgac cgtttctttt ttgagccaac accttaacct
      361 tgggggcctt tccttgtttg ccgtgtgcac gtccaccaga gtccctaacg ggggtggaaa
      421 gcctttgagg acaacagggc acccactttg gaaaaaggtt caaaaagggg gttgacacgg
      481 cataggcggg ggttcttttt tgtccgccat ttttccctgg atttttcctc atcgcctctt
      541 cccaccgaca tgccatgagt ccattgcgtg gcccggacat ttacgatccg ggagtgcgga
      601 gtggatagtt tctttgcatc ccgcccgagc ggatgacgtg cgggcgggat gaggccgtga
      661 tgacccggcc gtgacggatt ctagggaggt gaaacataaa ccatgtctag cggtttgatc
      721 attctattca tcgttttggc gggcgccgtc gggttggtcc tgggctatgc cctgaagcag
      781 aggctctccc ggaagcaacg cggcgcatcg gaacgcctcg gggcccgcat cgtcgacgag
      841 gccaagaagg aagcggaaaa catcaagaag gaggcgatcc tgcaggcgaa ggagaacctc
      901 ctcaaggcca agacggagtt cgagagggac gcccaggaga gaaagaccga gctcgacaac
      961 ctggaaaagc gcctgcgcac caaggaagag gcgatcgaca agcggatcga cacgatcgtc
     1021 cagaaggagg ccaatctgga ggggcgggac aaggccctga cccagaagga gtcccagatc
     1081 ggcgagaagc acggtcgcct ggaccggatg atcgaggagc agcgggagca actggaaaag
     1141 gcggccggca tctccgccga agaggcgaag gcgctcctcg ttcaggcgat ggagacggag
     1201 gccaagcacg aggcggccct ggccatccgc aagatcgagg acgagacccg gcggacggcg
     1261 gacaaaaagg cgcgggacat cctggcgtac tcgatccagc gctacgcggg cgacgtggtc
     1321 gcggaaaaca ccgtttccgt cgtgagcctg ccgaacgacg aaatgaaggg ccggatcatc
     1381 ggccgcgaag ggcgcaacat ccgggccatc gaggcggcca cggggatcga cctgatcgtg
     1441 gacgatacgc cggaggcggt cgtcctgtcg agcttcgatc ccgtgcggcg ggaggtggca
     1501 aggctttccc tggagcggct gatcacggac ggccggatcc atccgggacg catcgaggac
     1561 gtcgtcaaga aggtggcgac ggaagtggac agcatcatcc gggagacggg ggagcgcacc
     1621 tccttcgatg tcggcgtcca cgacatccat ccggagatcc tgaagctctt ggggagcctg
     1681 aagtaccgga cgagctattc gcagaacgtc ctccagcatt ccgtggatgt ggcgcatctg
     1741 acgggcatga tggcgtccga actcaagatg aacgtcaagg aggccaagcg cgccggactc
     1801 ctgcacgata tcgggaaggc catcgaccac aaggtcgagg ggccccatgc ggccatcggc
     1861 gccgattttg ccaagcgcta cggagagtcg ccgcggatca tacaggccat cgccacccat
     1921 cacgacgatg ggcggacgaa caccatcctg ggcgttctcg ttcaggccgc cgataccctg
     1981 tccgcggccc ggcccggggc ccggcgggag atgctggaga cgtatgtgcg gcggctggag
     2041 gaactggagg ccatcgcgaa ctccttcacc ggcgtggaca agtgtttcgc catccaggcg
     2101 gggcgggaga tccggatcct cgtggagaac gagaagattt ccgacaacga cgcggtcatg
     2161 ctctgccgcg acatcgtcaa gaagatcgag gccgaactga cctatccggg gcagatcaag
     2221 gtgacggtca tccgggaaac ccgcgtctcg gagttcgcga agtgaccggg gcgaagggca
     2281 ggggacaaag gcacatgcca actctccccc acgcctgtcc aaaggattat catgaacata
     2341 ctttttatcg gcgacatcat cggtaagccg gggcggcgcg ccgtgcggga gctgctgccc
     2401 cgccttgtgg acgaggcgag gatcgatttc gttatcgcca attgcgagaa tgcggcggcc
     2461 ggtttcggcg tgacccgcga cgtggtcgag gagctgtatc gtgacggcgt ccatgttctg
     2521 acctcgggaa accacttctg ggacaagaag gaggtcctcg aattcgcgga ggattacgaa
     2581 accctcctgc gtccggccaa ttatccggac ggcgtgcccg ggtggggcag cgcgattctg
     2641 ccgacgtcga ccggggaata cgtcggggtg ctcaatctgg ccggccgggt gttcatggcc
     2701 ccgctcgact gtccgttccg gacggcggac cgggagctcg cgcgcctgga gaaaaaggcc
     2761 cggatcatca tcgtggacgt gcacgccgag gcgacgtcgg agaagcaggc cctgggctgg
     2821 tacctggcgg gccgcgtcag tgccgtcatc gggacgcaca cgcacgtcca aacggcggat
     2881 gcttgtatcc gtcccggcgg gacggcttac ctgacggatg ccgggatgac ggggccgttc
     2941 gattcggtca tcggcatcgg caaggacatc atcctggagc gctttctctc cctgacgccg
     3001 agaaagtacg acgtggcgaa gggggacatc cgccttcagg gcgccgtcgt caccgtggac
     3061 gaaaagaccg gccgggcgac ggacatccgg gcgatcagcc ggaaactgga gacgggatag
     3121 attcaagacg gagacgcacc atgagcgttt ttgatgtttt tcgcgagcgg gggttcatcg
     3181 agcaggtcac ggacgaggcc ctggtcgagg acctgctcgc ccgggaaaag gtcacctgct
     3241 acatcggctt cgatccgacg gcggcgagcc tgcacgtcgg cagcctggtg ccgatcatgg
     3301 ccctcgccca catgcagcgc catggccacc gccccattgc cctgatcggc ggcgggacgg
     3361 ccctgatcgg cgatccgtcc ggcaagacgg agatgcgccg gatcctgacc cgggcgcagg
     3421 tcgatcacaa cgccgcctgc attcgcgagc agttgtccca gtatctcgat ttctcggacg
     3481 gtaaggccct gctgctgaac aacgccgact ggctggcccg gctcaattat atcgaatttc
     3541 tgcgcgacat cggccggcac ttcagcgtga acaagatgct ggccgcggag agttaccgga
     3601 tccgcctcga aaagggactc aacttcatcg agttcaacta catgctgctc caggcctacg
     3661 atttcctcca cctcttcaag caggagggct gcgtcctcca gatggggggc aacgaccagt
     3721 ggggaaacat gctggccgga agcgacctga tccggcgcct ggagggcaag aacgccttca
     3781 gcctgacgtt tcccctgatg accacctccg cgggccacaa gatgggcaag acggagaagg
     3841 gaacggtgtg gctggaccgg tcgctcacct cgccctatga ctattaccag tactggatca
     3901 acgccgagga cgccgacgtt gagcgcttcc tgctcctgtt caccttcctg cccgtggagg
     3961 aggtccgcgc ggtccgcgcg ttgagcgacg cccggctgaa tatggccaag gcggtcctcg
     4021 ccttcgaggc gacgaaggtc acgcacggcg aggccgcggc ggcggatgcc tggcgggcgt
     4081 cctgccaggc cttcggcacg aagccggtgg cggaggggct gttcccgtcc agcacgattc
     4141 cccgtcggga cgcggcccag gacgtatcgg ccatcccgtg cctcgtcaaa tcccgggacg
     4201 acatgggcgc cggcatcccg gcgtttgagc tctttcacga agccgggctg tgcgcctccc
     4261 gcggcgaggc ccggcgtctg atccagcagg gcggcgggta cgtgaacgaa cgccccgtcg
     4321 accggatcga cgagataatc ggcctcggcc actgtgacgc gcagggggaa atccgcttaa
     4381 ggaaggggaa aaaaacatat ttcatcgttc gcgtaaaata attcaaatta atgcttgaca
     4441 ccccggggaa gaaaggttaa aagccgcgtc tgttctttgg tagcaggctt ttactcgagc
     4501 ggttgtctcc tgggttcctg acgttttcac tctccggaaa ggcccgtctt ttccggaacg
     4561 gggttaaaaa ttccgtttaa aataatttga aattgttctt gacactcgga gagttttgaa
     4621 ttacacataa aagctcgttt gaagcacgtt ctttcaaaag tgaatagtgg gataatcgat
     4681 tagcaggtc
//
LOCUS       DAOOLJ010000102         4557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_22879_length_4557_cov_16.001555, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000102 DAOOLJ010000000
VERSION     DAOOLJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22879_length_4557_cov_16.001555"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLW16_09985"
     CDS             <1..542
                     /locus_tag="PLW16_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HPB71277.1"
                     /translation="VARKIREYGIEKLVVDPVMIAKGGTPLIRDEAKPPLLAELIPLA
                     FVVTPNVPEAEILAGMKIASLADMEEAARRIHRAGARHVVVKGGHLNRGLGEATDVLY
                     DGREVHRLASPWIATRDTHGTGCTFSAAIATGLAHGKDVPDAVAAAKRYITEAIRHAW
                     RIGGGHGPTNHMAGVKGEG"
     gene            593..1783
                     /gene="cytX"
                     /locus_tag="PLW16_09990"
     CDS             593..1783
                     /gene="cytX"
                     /locus_tag="PLW16_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HPB71278.1"
                     /translation="MEKRPQLSGVNLFFLWFGAAVSVAEILAGGFLADLGLVRGLWAI
                     VLGHAVGVALLVLAGLIGFNEKLPAIMSTRISFGRQGSYLISAINILQLIGWTAIMIV
                     EGGVAMNAIAKALWQFNHPAAMMIGIGVLIGLWVFWDVEGFRYINTIAAVFLLGLTVV
                     MSFVLLTRPDIAAATASRSPGLFGMGFELAVIMPLSWVPLISDYTSLAPSRRAAVAGP
                     LIGYFIGSCWMYAIGLVGAIYAGTSDPTGVMLAANLGLAALAILVLSTVTTTFLDVFS
                     AGVSLLNIFPRLSRRWVAVLFTVLGTGLALVFPIDRYTDFLYVLGSVFSPLVAVLLCD
                     YFLLKRDNRERRADLAATISLLVGIGFYYLIKPFPIPVGPTLTTIAFTALLHLSVRTV
                     FRRV"
     gene            complement(1817..2791)
                     /locus_tag="PLW16_09995"
     CDS             complement(1817..2791)
                     /locus_tag="PLW16_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB71279.1"
                     /translation="MRAFVTGAGGFIGSFLCRALQEKGWEIVAFALPEEQVDSLTAAG
                     IGVRRGDLTEPGTLRGCCEGIDVVFHLAGRVVDWGSREVFYANIHAATKHLLEEAAGR
                     VSRFVYISSIAACGLGRHLKGRREADPCFKSGVPYNDAKLDTEALVRRYQTAKGMDAT
                     VIRPANVIGPGSVWVRDVLDSYRRLTVPLIDGGRWSASLVYVENLVDGIVRASTQEAG
                     RGKTYQFRDDWNVTWKQYLTDLGAMIGKAPGFNIPFSVAWTAGAVCETVLTPLNLRAP
                     ITRLAAGVMGRDNDVDTTKARTELGWQTVVSYPEALRRIGVWVRERYG"
     gene            complement(2889..3974)
                     /locus_tag="PLW16_10000"
     CDS             complement(2889..3974)
                     /locus_tag="PLW16_10000"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPB71280.1"
                     /translation="MKKHPLLSGLVGIAVLLALYLSFWPVAIDPAPWVPPAAPERTGV
                     YTPNNLLSGVQRLESGMGSPLEGVAIDRERRLYAGTKDGRIIRYDREGKTPEVFVEIG
                     GWPLGIAFDAAGNLIVCNGRNGLRSVASDGTVTTLTVEQGGVPFRNVDGLAIAPDGTI
                     YFTDVSKKFTDDQYMDDLLEHRPNGRLLAYDPATRKTRLVLDGLYFPNGVAVAPDNTF
                     LILAEMGTYRLLRIRLSGPRTGTSEVFIDNLPGFPDGLFPGSDGVFWVTIVSPRDKLA
                     DTVLFPHPPLRKVLRRLPRALMPAPKNYGYVLGLDGNGTIVHNLQDPTGTFGQITNVL
                     EHDGKLYMGSLSEDAIGVLPVPKRAVK"
BASE COUNT          726 a         1500 c         1498 g          833 t
ORIGIN      
        1 tcgtcgcccg gaaaatccgc gagtacggca tcgagaagct cgtcgtggac cccgtgatga
       61 tcgccaaggg gggcacgccc ctgatccgcg acgaggcgaa gccccccctc ctcgcggagc
      121 tgattcccct cgcctttgtt gtgaccccga acgtcccgga ggcggagatc ctggcgggga
      181 tgaagatcgc ctcgctggcg gacatggagg aggcggcccg gaggatccac cgggccggcg
      241 cgaggcacgt cgtcgtcaag ggcgggcacc tgaaccgcgg cctgggtgag gcgaccgacg
      301 tcctctacga cgggcgggag gtccatcgtc tggcctcgcc gtggatcgcg acgcgggaca
      361 cgcacgggac gggctgcacc ttctccgccg ccatcgccac gggtctcgcc catgggaagg
      421 acgttccgga cgccgtcgcg gcggcgaaac gctacatcac cgaggccatc cgtcatgcct
      481 ggcggatcgg cggcggccac ggcccgacga accacatggc gggggtaaag ggtgaaggat
      541 aagaggcaaa aggccgaacg ccccgcccca atgccgggat aggaatattt gcatggaaaa
      601 gcggcctcag ctcagcggcg tcaacctgtt tttcctctgg ttcggggccg ccgtgtccgt
      661 cgcggagatc ctggcggggg gatttctcgc ggatctgggg ctcgtcaggg ggctctgggc
      721 cattgtcctc ggtcatgcgg tcggggtggc cctcctcgtg ctggccggcc tcatcgggtt
      781 caacgaaaaa ctgcccgcca tcatgtccac gcggatctcg ttcgggcggc aagggtccta
      841 cctgatttcc gcgatcaaca tcctccaatt gatcggctgg acggcgatca tgatcgtcga
      901 gggcggcgtg gccatgaacg ccatcgcaaa ggccctgtgg cagttcaatc accccgcggc
      961 gatgatgatc ggaatcggcg tcctcatcgg cctgtgggtc ttctgggacg tcgaagggtt
     1021 ccgctacatc aacacgattg ccgcggtttt cctgctcggc ctgaccgtcg tgatgagctt
     1081 cgttcttcta acccgtcccg acatcgcggc ggcaacggcg tcccggtccc cgggcctgtt
     1141 cggcatgggg ttcgagctgg ccgtcatcat gccgttgagc tgggtccccc tgatttccga
     1201 ctacacctcg cttgcgccgt cgcgtcgcgc cgccgtggcg gggcccctga tcggctattt
     1261 catcgggagt tgctggatgt acgcgatcgg cctcgtgggc gcgatctacg cggggacatc
     1321 cgatccgacg ggcgtgatgc tcgcggccaa cctggggctc gcggccctgg ccattctcgt
     1381 cctgtccacg gtgacgacga ccttcctcga cgtcttctcg gcgggcgtgt cgctgctgaa
     1441 catctttccc cgcctgtctc ggcgttgggt cgccgtgctc ttcaccgtcc tcgggaccgg
     1501 gcttgccctg gtgtttccga tcgacagata caccgatttt ctctacgttc tgggatcggt
     1561 tttttcgccc cttgtggccg ttctcctgtg cgactatttt ctcctgaagc gggacaaccg
     1621 cgaacgccgg gccgacttgg cggcgaccat ttccctgctg gtcggtatcg gcttctatta
     1681 tctcatcaaa cccttcccca tcccggtcgg gcccacgctc accaccatcg ccttcacggc
     1741 gctcctccac ctgtccgtgc ggacggtctt caggcgggtg tgatcccggg cgggtccggg
     1801 ggccgattcc cggccgtcag ccgtaccgct cccggaccca gacgccgatc cgccgcaggg
     1861 cctcggggta ggagacgacc gtttgccagc ccagttccgt ccgtgctttc gtcgtgtcaa
     1921 cgtcgttgtc cctccccatc acaccggcgg ccagacgggt gatgggggcg cggagattca
     1981 gcggcgtgag gaccgtttcg cacacggccc ccgcggtcca ggcgacggaa aaggggatgt
     2041 tgaaacccgg ggcctttccg atcatggcac ccagatcggt caggtattgc ttccatgtga
     2101 cgttccagtc gtcccggaat tgataggtct tgccgcggcc ggcctcctgc gtgctggccc
     2161 ggacgatccc gtccacgagg ttctccacgt agacaagact ggcgctccag cgtcccccgt
     2221 cgatgagggg cacggtgagt ctccggtagc tgtccaggac atcgcggacc cagacgcttc
     2281 cggggccgat cacgttggcg ggccggatca cggtggcgtc catgcccttc gcagtctggt
     2341 aacgccggac gagggcctcc gtgtcgagct tcgcgtcgtt gtagggcacc cccgacttga
     2401 aacaggggtc cgcctcccgg cgccccttca ggtgccgccc caggccgcag gccgcgatgg
     2461 agctgatgta gacgaagcgg gatacccggc ccgcggcctc ctccaggagg tgcttcgtcg
     2521 ccgcatggat gttggcgtag aagacctcgc gggaccccca atccacgacg cggccggcca
     2581 agtgaaagac cacgtcgatc ccttcgcagc agcccctcag cgtcccgggc tcggtgaggt
     2641 cgccgcgccg gaccccgatg ccagcggccg tcagggaatc gacctgctcc tccggcaggg
     2701 cgaaggcgac gatctcccac cccttctcct gaagcgcccg gcagagaaac gaaccgatga
     2761 acccgcccgc tcccgtcacg aatgcccgca tgtcatgcct cctttcatca gccggtcccg
     2821 gattcccgaa aacggggccg gtttggaaac cccggacgga acccgccctc gcaaaccgaa
     2881 atcttcgttc atttcaccgc gcgtttcggc acggggagga ccccgatggc gtcctccgac
     2941 aggctgccca tgtagagctt tccatcgtgt tccagcacat tggtgatctg gccgaaggtg
     3001 ccggtcgggt cctggaggtt atgaacgatc gtgccgttgc cgtccaaccc caggacgtaa
     3061 ccgtaatttt tcggcgccgg catgagggcg cggggcaggc gacggagcac cttgcggagg
     3121 ggcgggtggg ggaagagcac cgtatcggcg agtttgtcgc gcggcgagac gatcgtcacc
     3181 cagaaaacgc cgtcgctccc ggggaacagg ccgtccggaa agcccggcag gttgtcgatg
     3241 aaaacctcgg aggtcccggt ccgggggccg ctgagccgga tgcggaggag ccggtacgtc
     3301 cccatttccg ccaggatcag gaacgtgttg tcgggggcga ccgcgacccc gttggggaaa
     3361 tagaggccgt ccagcacgag ccgtgtcttg cgggtcgccg gatcataggc caggagcctc
     3421 ccgttgggtc ggtgctccaa aagatcgtcc atgtattgat cgtcggtgaa tttcttcgac
     3481 acgtccgtga aatagatcgt cccgtccggg gcgatggcga ggccgtccac gttccggaag
     3541 gggacgccgc cctgctcgac cgtgagggtc gtgaccgtcc cgtccgacgc aacggaccgc
     3601 agcccgttcc tgccgttgca gacgatcagg tttccggccg cgtcgaaggc gatgccgagc
     3661 ggccaccccc cgatctcgac aaagacctcg ggggttttcc cctcccggtc gtaccggatg
     3721 atgcggccgt cctttgtccc ggcgtagagc cgtctctccc ggtcgatggc cacgccttcc
     3781 agcggcgatc ccatccccga ttcgaggcgc tggacgcctg acagcaaatt gttcggcgta
     3841 tagacgcccg tccgttccgg tgcggccggg gggacccacg gcgcggggtc gatggcgacg
     3901 ggccaaaagg agaggtaaag ggccagcaga accgcgatgc cgacaagacc ggacagcagg
     3961 ggatgtttct tcataagaga cctcctgatg gctggggcga ccggttgtga gacggagaga
     4021 acgttttccc ccgacccggc gggccgaaac cggccccttt ggggcgtcgg gccgaccggc
     4081 gcgatcggac gatccgaatc tggaatgccg tcaatcttgc atacattgaa cggtccgtca
     4141 atcgacgact ttacagtgat tcatgccggc cgggattcta tgttgcggaa tctattcttt
     4201 tcttgtaaca actggcctga gaaccgccaa gctgtgggct caggattgag gaacggagcc
     4261 caatgggggg cggcggcgcg tccatcggca aacgggttga agggcggatt cgcacggcag
     4321 gtcccggtcg tgatcggggg gctgacaggc gtttgcaggg ggactgaagc ggcgttcccg
     4381 tcgagtatcc ggcttcgcgc gagagaaacc ctcacatgga tcggcttgct cagatgcatc
     4441 cacggaacat ccctcccgag gtctcttcac ccctccccgc gctgcgggaa ggggcggcgg
     4501 ccatcgtgcg cgccctgcgc cgcgccggcc acgaggccta ctgggtcggc ggctgcg
//
LOCUS       DAOOLJ010000103         4518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_23143_length_4518_cov_12.391217, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000103 DAOOLJ010000000
VERSION     DAOOLJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23143_length_4518_cov_12.391217"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLW16_10005"
     CDS             <1..476
                     /locus_tag="PLW16_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71281.1"
                     /translation="KYTPRHKKKVLININYQSISINQFQSIQLSMTGDIGYVDQDGYL
                     FIVDRKKDMLLCKGHNVYPRDLEEVLNAHPDVMQAAVVGKKDERYGEAPVAFVQLRPG
                     ATATEVELLTYANENMAAYKKIRRLVILAQLPASAAGKVLRRELRDQVQTSDFVV"
     gene            585..1994
                     /locus_tag="PLW16_10010"
     CDS             585..1994
                     /locus_tag="PLW16_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71282.1"
                     /translation="MADYPRKPKVVSEFGQFLYKSQDEIRAVQNEKLRHQVQLLEKYS
                     PYYSKLFQEKGITAADIRTVDDLEKIPLLYKKDYMADPMSFRLVIPADAPVQPHERIL
                     WGVHYTTGTTSGVPTPFFSMTHDYFGNLMQLARMDEVGGITADDTVANLFPLTPVPHI
                     GFWKTVDYSIACAAKVCNALTGSGYPPFPIHRPLDYAVEMVVKNRATVLSGIVSFVRR
                     VIMRAEELGKDFSSVRMCFVLGEACPKGMREDMRTRLKNIGAKDPFICSGLGFTEMQG
                     TTLECSEGGGMHLSAPDLHFLEIVDEETGKRLPDGEKGLLTITHVDRRGTCFLRYVVG
                     DITTLSHETCPHCGRNDVRVVMQPIRTMELIKVKGTLINPDILKTEITAIKGIEEYQI
                     VFTKEDLKDPLSLDKLLIRVAAAAGFDQAEMQTNIKTATKRAVEMTPDVVFVDKYDIF
                     DPTKTLKSTRVVDERPPVE"
     gene            2021..3424
                     /locus_tag="PLW16_10015"
     CDS             2021..3424
                     /locus_tag="PLW16_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB71283.1"
                     /translation="MTEKYDTMIIGAGIAGLGVGAILTKEAGQKVLVLDRFPKFGGRM
                     MSYDGYPAAGWRVEIGLHMIELGEKSSCNALNERVGKKVAWAPFSETVDIWNGEKFVS
                     VAELVPMTKEDKVAFRELMQKIATLSDAEIEVWDNRSLAEWLYENVPQAPVRELFTDL
                     GMIMTTIPHAIDMAAGEVLFIARENLLKTRQALQASYPLDGMEGVTRGLVETIRAGGG
                     EIRLNCDVQEVVIENGTAVGVRIPSQKHIYQEEYRIFETETLLADRVVCALPIYKLSQ
                     IIDFNPESSPLPRWWIKRITDIQHEVTGLIGFMIGLKEPVTDPKKKCFFSALKTRHAG
                     FPFQAFPASNFSDNVAPVGKQVIHTDLVCEHAEASDKFKRERLLKLHWEDLKEMFPGI
                     EDKMEWRLPYYVDGCDGLARQPGLVGNFKPALQAPGIPNLYFTGDTYLGRGLAINSAA
                     RSGMLCADLILKTLNGK"
     gene            3470..>4518
                     /locus_tag="PLW16_10020"
     CDS             3470..>4518
                     /locus_tag="PLW16_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPB71284.1"
                     /translation="MAERDLSFMRTFGYPEKWLEESSTYGVNDSYLITQPGWSVEPIP
                     PIPEISIYEIFAASARKYPDDVAVIFLDRKITYRDMDDMINRYASLLLDLGVKKGDVV
                     ATMLPNCFQHWIAFFGANRIGAAHTPLNVMYKDREIAYQIKDSGAKTIVTYDLFYQLY
                     FAKLRDEIGLKNVIVTHVKDFAAPDTNAEALGMLKLFWDAPKQPLPGTVDLFDAIASH
                     QPTQAKVACNPREDTALILYTSGTTAAEPKGVIETHFNLVFNALSHFHMMNALKKREV
                     NFSIMPMFHTAGYFLHTLPVFYQGGTVIPIPLFNPDEALRVISTHQVSTIFAPPTLYI
                     ALMQHPRISEVDLSSL"
BASE COUNT         1082 a         1263 c         1276 g          897 t
ORIGIN      
        1 ctaaatatac tccacgacac aaaaagaaag ttcttatcaa tatcaattat caatcaatat
       61 caatcaatca atttcaatca attcaattat caatgaccgg cgacatcggg tatgtggatc
      121 aagacggtta cctgttcatc gtggaccgca agaaagacat gctgctgtgc aagggacaca
      181 acgtctaccc gcgggacctg gaggaggtct taaacgccca tccggacgtg atgcaggcgg
      241 ccgtcgtcgg gaagaaagac gaacgctacg gggaggcgcc ggtggctttc gtccagttgc
      301 ggcccggcgc aacggcgacg gaggtggagc tcttgacata cgccaatgag aacatggccg
      361 cttacaagaa aatccgccgg ctggtgattc tcgctcagct ccccgccagc gccgccggaa
      421 aagtcctccg gcgggagctg cgcgatcagg tgcaaacttc tgattttgtt gtatgagtca
      481 aatgttaggg gatcaaacaa gacggcacgg aataacgatg cttcggacat gacgagggat
      541 cgccctggag ggggtgcgac aaataaaaaa ggaggacgat tgacatggct gattatccaa
      601 gaaaaccaaa ggttgtcagt gagttcgggc agtttctcta caaatcgcag gatgaaatcc
      661 gggccgtaca gaacgaaaag ctccgccacc aggtccaact cctggagaag tacagtccct
      721 attacagtaa acttttccag gaaaagggca tcaccgcggc ggatatccgg acggtggacg
      781 atctggagaa gattccactt ttgtacaaga aagattatat ggccgatccg atgagtttcc
      841 ggctggtcat tccggccgat gcgccggtgc agccccacga gcggatcctc tggggcgtcc
      901 attacacgac cggaacgacc tccggcgtac ccacgccgtt cttctcgatg acgcacgact
      961 acttcgggaa cctgatgcaa ttggccagaa tggacgaggt cggggggatt accgccgatg
     1021 acaccgtggc caatctcttc ccgctgaccc cggttccgca cattggattc tggaagaccg
     1081 tcgattactc gatcgcctgt gccgccaagg tctgcaacgc cctgacgggt tccggctatc
     1141 cgccgtttcc gattcaccgg ccgctggatt acgcggtgga aatggtcgtg aaaaaccgcg
     1201 ccacggtgct ctccggcatc gtttccttcg tccggcgcgt aatcatgcgg gccgaagaac
     1261 tgggcaagga cttttccagc gtcaggatgt gctttgtgtt gggcgaggcc tgtccgaagg
     1321 gcatgcggga ggatatgcgg acccgcctga aaaatatcgg ggcgaaagat ccctttatct
     1381 gcagtggcct cgggtttacc gagatgcagg gaacgacgct ggaatgcagc gagggcggcg
     1441 gcatgcactt aagcgctccg gatctccatt ttttggaaat cgtggatgag gagaccggca
     1501 agcgcctgcc cgatggggag aaggggctgc tgacgatcac ccatgtggac cggcgcggca
     1561 cttgctttct gcgctatgtc gtcggggaca tcacgacgtt gagtcatgag acctgccccc
     1621 attgcgggcg gaacgacgtc cgggtcgtca tgcagccgat ccgaacaatg gaactgatca
     1681 aggtgaaggg gaccctcatc aatcccgata tcctgaaaac ggagatcacc gccatcaagg
     1741 ggattgaaga atatcagatc gtcttcacga aggaagatct taaagatccc ctttcgttgg
     1801 acaagcttct gatccgtgtc gcagcagcgg cgggattcga ccaggccgaa atgcagacga
     1861 acatcaagac cgccacgaag agggccgtag aaatgacgcc ggatgtggtg ttcgtagaca
     1921 agtacgacat cttcgatccg acgaagacct tgaagagcac ccgtgtggtg gatgaacgac
     1981 cgccggtgga gtaaaagaat tccaaaagga gaaagaaatc atgacggaaa aatatgatac
     2041 gatgatcatc ggggccggga ttgccggcct gggtgtcggg gcgatcctga cgaaagaagc
     2101 cggacagaaa gtgctcgtgc tggaccgctt tccaaaattc ggcggccgga tgatgagcta
     2161 cgacggctat cctgccgcgg gctggcgggt ggagatcggc ctgcacatga tcgagttggg
     2221 agaaaaatca agctgcaacg cgttgaacga aagagtcggc aaaaaggtgg cctgggcgcc
     2281 gttcagcgaa actgtagaca tctggaatgg ggaaaagttc gtcagtgtgg cggaactcgt
     2341 cccgatgacc aaggaggaca aggtcgcgtt ccgggagctg atgcagaaga tcgcgacttt
     2401 aagcgacgcc gagatcgaag tgtgggacaa ccgatcgctc gcggaatggc tttacgaaaa
     2461 cgttccccag gcccccgtcc gggaactctt caccgacctc ggaatgatca tgacgaccat
     2521 tccccatgcc atcgatatgg ccgcaggcga agtccttttc atcgcccgcg agaacctgct
     2581 gaagacccgc caggccctgc aggcgagcta tccgctggat gggatggaag gggttacccg
     2641 gggactcgtc gaaaccatcc gggcgggggg cggagagatc cgccttaact gcgacgtgca
     2701 ggaagtggtc atcgagaacg gcacagccgt tggtgtccgc attccctccc agaagcacat
     2761 ctaccaagag gaataccgga tcttcgagac cgagacgctt ttggccgacc gcgtcgtgtg
     2821 cgcgctgcca atttacaagc tctcccagat catcgatttc aaccccgaat ccagccccct
     2881 gccgcgttgg tggatcaaaa ggattacgga catccaacat gaagtgacag gactgattgg
     2941 gttcatgatc gggctgaaag aaccggtgac agacccaaag aagaaatgct tcttcagcgc
     3001 actgaagacc cggcatgccg gtttcccgtt ccaggcgttc ccggcttcga atttctccga
     3061 caacgtggcc ccggtgggca aacaggtcat ccatacggac ctggtctgtg agcacgcaga
     3121 ggcctcggac aagttcaagc gggagcggct ccttaaactc cactgggaag acctgaagga
     3181 gatgttcccc gggatcgagg acaaaatgga atggcgcctg ccctactacg tggacggctg
     3241 cgacggcctg gcccgtcagc cggggcttgt gggaaacttc aagccggcct tgcaggcgcc
     3301 gggcattccg aacctctatt ttaccggtga tacctacctg ggccgggggc ttgccatcaa
     3361 cagcgccgcc cggagcggca tgctttgcgc cgatttgatc ctgaaaacgc tcaatggaaa
     3421 atgaactgca atcatcagca gcgaaatcga actaggagga atggatgtaa tggcagaaag
     3481 agatctcagt ttcatgcgga cgttcggtta cccggagaaa tggctcgaag aatcttcaac
     3541 ttacggtgtc aacgattcct acctcatcac ccagccgggc tggtcggttg aacccatccc
     3601 gcccatcccg gagatttcca tctatgagat ttttgccgct tcggcccgga agtatccgga
     3661 cgatgtggct gtaatttttc tcgaccggaa aatcacctac cgggacatgg acgacatgat
     3721 caaccggtac gcttccctgc tccttgactt gggtgtcaag aagggggacg tcgtcgccac
     3781 gatgctgccc aattgttttc agcactggat tgcttttttc ggtgccaacc gtatcggagc
     3841 cgcccacacg ccgttgaacg tcatgtacaa agatcgcgag atcgcctatc agatcaagga
     3901 ctccggggcc aaaaccatcg tgacctatga cctcttttac cagttgtact ttgccaagct
     3961 gcgcgacgag atcggcttaa agaacgtgat cgtgacccac gtgaaggatt tcgccgcccc
     4021 ggatacaaat gccgaagccc tgggaatgtt gaagctcttc tgggacgccc cgaaacagcc
     4081 cctaccggga accgtcgatc tcttcgacgc catcgccagc catcagccca ctcaggcgaa
     4141 ggtcgcatgc aatccccgcg aggacacggc cctcattctt tacacctccg gaacgactgc
     4201 ggccgaaccc aagggggtca ttgaaacgca tttcaatctc gtcttcaacg ccttaagtca
     4261 tttccacatg atgaacgccc tgaagaaacg tgaagtcaat ttctccatca tgccgatgtt
     4321 ccatacagcc ggctattttc tgcataccct acccgtcttc taccagggcg gtaccgtcat
     4381 tcccatcccc ctcttcaatc ccgacgaggc cttacgggtc atcagcaccc atcaggtcag
     4441 caccatcttt gccccaccga ccctttacat cgccttgatg cagcatcccc ggatcagcga
     4501 ggtcgacctg agcagcct
//
LOCUS       DAOOLJ010000104         4282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_24816_length_4282_cov_21.168204, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000104 DAOOLJ010000000
VERSION     DAOOLJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24816_length_4282_cov_21.168204"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="pdxT"
                     /locus_tag="PLW16_10025"
     CDS             complement(<1..527)
                     /gene="pdxT"
                     /locus_tag="PLW16_10025"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPB71285.1"
                     /translation="MIPIRAPRAPEIGILDLQGGIQEHLDHLERLGIDARRIKTADAL
                     SDVAGLILPGGESTCIARLLAIFGLDQVILREVRRGMKLWGTCAGTILVAKTLVGEPA
                     HLGLIDITVERNGFGSQLDSFAAEAVIPKVSPDPIPLTFIRAPKITHVGPGVDILLQI
                     DDYIAAAESPDVLVTV"
     gene            complement(538..1419)
                     /gene="pdxS"
                     /locus_tag="PLW16_10030"
     CDS             complement(538..1419)
                     /gene="pdxS"
                     /locus_tag="PLW16_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPB71286.1"
                     /translation="MDAKRYELNVQLAQMLKGGVIMDVTNVEQAKIGEEAGAVAVMAL
                     ERVPADIRRDGGVARMSDPAMIAQIKQAVSIPVMAKARIGHFVEAQILEALKIDYIDE
                     SEVLTPADEECHIDKTRFAIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMNREIRQLAAMPVEEVTGFAKANGMPYELALKVKTLGRLPVVNFAAGGIATPA
                     DAALMMQLGCDGIFVGSGIFKSADPARRARAIVKATAYFNDPARVLEASMDLGEAMPG
                     LEIASIPEEQRLAGRGW"
     gene            complement(1526..2308)
                     /gene="hisF"
                     /locus_tag="PLW16_10035"
     CDS             complement(1526..2308)
                     /gene="hisF"
                     /locus_tag="PLW16_10035"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPB71287.1"
                     /translation="MLRKRIIPCLDVRAGKLTKGIKFKGNIDIGDPVEAARQYYEQGA
                     DEIVFYDITASSDQRQIMLDVVRRVAATIFIPFSVGGGIRTLADMRAVILAGAEKVSV
                     NSAAVQNPRLIAEGAKAFGSQCIVLGMDVKRVEASARIPSGYEIVIHGGRIDTGRDAL
                     EWAREAECLGAGEICLNSIDADGTRDGYEIRLTSLISDRVGIPVIASGGAGTPEHLHD
                     VLTRGRADAALIASMVHYRTHTLADIKAYLSGRGVPVRVHWA"
     gene            complement(2298..2936)
                     /gene="hisH"
                     /locus_tag="PLW16_10040"
     CDS             complement(2298..2936)
                     /gene="hisH"
                     /locus_tag="PLW16_10040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPB71288.1"
                     /translation="MIGIVDYRAGNLTSVARALEHLHEPCEVTDRPEVLDRASHIIFP
                     GVGAAAEAMANLRRSGLDAWLKKWVQEGRPILGICLGTQVILSESEEDGTTPGLGIIP
                     GVVKKFPGTLQAEGQRLKIPHMGWNTVEFQGDHPVFAGLPRAAEFYFVHSYYPAPMDE
                     RLVVGWTRYGIRFCAALARDNLVAVQFHPEKSGRPGLRILANFCRWGGNDAA"
     gene            complement(3100..4068)
                     /locus_tag="PLW16_10045"
     CDS             complement(3100..4068)
                     /locus_tag="PLW16_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418923.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPB71289.1"
                     /translation="MGKPAFWNNTKRYYDLKSFWLNAFGHRVYKLPIDAGFTCPNRDG
                     TVARGGCIYCDGRGSALRQAGPLPAVAEQIRKGRAYYREARHAGRFIAYFQTFTNTYG
                     SLEHLRALYDEALAQEDIIGLSVGTRPDCVPDAVLDLLQDYARDRHVWLEFGLQSIHD
                     RTLAALNRGHTAAQFLDAVARTAGRGIRVCVHIIVGLPGESHADILETARVLASLPVQ
                     GIKIHALLALKGTVLGDRYARGEIQTVSREHYVGTVCDVLELLPPDLVVQRLTADGYK
                     DIFLGPPWAVNKMAVLNAIDRELERRDAWQGCRSAPRPRSGSEDMR"
     gene            4053..>4282
                     /locus_tag="PLW16_10050"
     CDS             4053..>4282
                     /locus_tag="PLW16_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB71290.1"
                     /translation="MPVCPRGLLFRCVLRRILLDSPFDCKDLVRGGGHLQSYDVIVVG
                     GGHAGCEAALAAARMGCETLLCNINLDAMALMP"
BASE COUNT          678 a         1432 c         1428 g          744 t
ORIGIN      
        1 accgtgacga ggacgtccgg agattcggcc gcggcgatgt agtcgtcgat ttggagcagg
       61 atgtcgaccc ccggcccgac atgcgtgatc ttgggggccc ggatgaaggt cagggggatc
      121 gggtccgggg acaccttcgg gatcacggcc tcggcggcaa aactgtccaa ctggctaccg
      181 aaaccgttgc gctccacggt gatgtcgatg agtccgaggt gcgccggctc gcccacgagg
      241 gtcttcgcga cgagaatggt cccggcgcag gttccccaga gcttcatgcc ccgccgaact
      301 tcccggagaa tcacttggtc gaggccgaaa atggcgagca gccgggcgat gcaagtgctc
      361 tcgccgccgg gtaggatgag gccggcgacg tccgacagcg cgtcggcggt cttgatcctg
      421 cgggcgtcga tgcccagacg ctccagatga tcgaggtgtt cctggatgcc gccctggagg
      481 tcgagaatcc cgatctccgg cgcacgcggc gcccggatgg gaatcacggg ggacatctca
      541 ccagccccgt ccggcgaggc gctgttcttc cggaatggag gcgatctcca ggccaggcat
      601 ggcctccccc agatccatgg aggcctccag gacccgggcc ggatcgttga agtaggccgt
      661 cgccttgacg atggcccggg cgcgccgggc cggatccgcc gacttgaaga ttcccgaacc
      721 gacaaagatc ccgtcgcagc caagctgcat catgagcgcc gcgtccgccg gggtggcgat
      781 cccgcccgca gcgaaattga cgacgggcag gcggccgagg gttttgacct tcagggccag
      841 ctcatagggc atgccgttcg ccttggcgaa gccggtgacc tcctcgacgg gcatggccgc
      901 gagctgccgg atctcccggt tcatcgtccg catgtggcgg accgcctcga cgacattgcc
      961 ggttcccgcc tcgcccttgg tccggatcat ggccgccccc tcggcgatgc gccgcagggc
     1021 ttcgcccagg ttccgcgcgc cgcagacgaa ggggatggcg aagcgcgtct tgtcgatgtg
     1081 gcactcctcg tccgcggggg tcaggacctc gctctcgtcg atgtaatcga tcttcagggc
     1141 ctcgaggatc tgggcctcca cgaagtggcc gatccgggcc ttggccatga cggggatgga
     1201 aaccgcctgc ttgatctggg cgatcatggc cgggtccgac atgcgggcaa cgccgccgtc
     1261 gcggcggata tccgcgggga cccgctccag ggccatgacg gccaccgccc ccgcctcttc
     1321 gccgatcttc gcctgttcga cattcgtcac atccatgatg accccgccct tcaacatctg
     1381 ggcgagctgg acgttcagtt cataccgttt tgcatccatg gtggcaccct ccccccgggg
     1441 ctggaacccg ccccgtcgtg aatcgtctta gtctatgtaa aggccgggga ttcgtcaagc
     1501 acaaacatga acctcccgcg cggcgctacg cccagtgcac gcggaccggc acgccgcgcc
     1561 cggagaggta ggccttgatg tcggcaaggg tgtgggtccg gtagtggacc atggaggcga
     1621 tcagggccgc atcggcccgg ccccgagtca ggacgtcgtg gaggtgctcc ggcgtgcctg
     1681 caccgccgga ggcgatcacg gggatgccga cccggtctga gatcagcgac gtcaggcgga
     1741 tttcataacc gtcccgggtg ccgtcggcgt cgatggaatt gaggcagatt tcccccgcgc
     1801 ccaggcactc cgcctcccgg gcccattcca gggcgtcgcg acccgtatcg atccggccgc
     1861 cgtgaatgac gatctcgtag ccggacggga tccgggcgga ggcctcgacc cgtttgacgt
     1921 ccattcccag gacgatgcac tggctcccga aggcctttgc cccctcggca atgagcctcg
     1981 gattctgcac ggctgccgaa ttgacgctga ccttttccgc cccggccaga atgaccgccc
     2041 gcatgtcggc cagggtccgg atgccgccgc cgacggaaaa agggatgaag atcgtcgcgg
     2101 cgacgcgccg gacgacgtcg agcatgatct gccgctgatc ggacgaggcc gtgatgtcgt
     2161 agaagacgat ctcgtcggcc ccctgctcat agtactgccg cgccgcctcc accgggtccc
     2221 cgatgtcgat gttgcccttg aattttatgc ccttggtgag cttgcccgcc cggacgtcca
     2281 ggcaggggat gatccgctta cgcagcatcg ttgcctcccc agcggcagaa gttggccagg
     2341 atgcggagcc ccggccggcc gctcttttcc gggtggaact gcacggcgac caggttgtcc
     2401 cgcgccaggg cggcacaaaa acgtatcccg tagcgtgtcc agccgacgac gaggcgttcg
     2461 tccatcggcg ccgggtagta cgagtggacg aagtaaaatt cagccgcccg gggcaagccc
     2521 gcgaacaccg ggtgatcgcc ctggaactcg acggtgttcc accccatgtg ggggatcttc
     2581 agccgctgtc cctccgcctg caacgtcccg ggaaatttct tgaccacgcc cggaatgatg
     2641 ccgaggccgg gcgtcgtgcc gtcctcctcg ctctcactca ggatcacctg ggtgccgagg
     2701 cagatgccga ggatcggcct gccctcctgc acccattttt tgagccacgc gtcgaggccg
     2761 ctccgacgca ggtttgccat cgcctcggcc gccgccccga cgccggggaa gatgatgtgg
     2821 ctcgcccggt ccaggacctc cggccgatcc gtcacttcgc agggttcatg caggtgttcg
     2881 agggcgcggg ccacgctcgt caggttcccc gcccggtaat ccacgatgcc gatcataacc
     2941 atccttcaac gaacgtcggt gacggcgccc ggccgtcccg tcgacgcctc tctccccatt
     3001 cccgtgaggg gctgcggacg ggaagcttcg tgccgggcgg cctccccgaa caggcccctg
     3061 ccgtttcaac ccaaaaaatg agcaaatagc gtgccgtcgt cagcgcatgt cctccgaacc
     3121 gctacggggc cgtggggcgc tacggcagcc ctgccaggcg tcccgccgtt ccagctcgcg
     3181 atcgatcgcg ttcagaacgg ccatcttgtt gaccgcccac ggcgggccca gaaagatgtc
     3241 cttgtacccg tcggcggtca gacgctggac gacgaggtcc ggcggcagga gttccagaac
     3301 gtcgcagacc gttccgacgt agtgctcccg gcttacggtc tggatttccc cccgggcgta
     3361 ccggtcgccc aggacggtcc ccttcagggc cagaagcgcg tggatcttga ttccctgcac
     3421 gggcagggag gcgagcacgc gggcggtctc gaggatgtcc gcgtggctct cccccggcag
     3481 gccgacgatg atgtggacgc agacgcggat cccccggccg gcggtccgcg cgaccgcgtc
     3541 caggaactgc gccgccgtgt gtccccggtt cagcgcggcc agggtccggt cgtggatcga
     3601 ctggaggccg aattcgagcc agacgtggcg gtcccgggcg tagtcctgca aaagatccag
     3661 gaccgcgtcc ggaacgcagt ccgggcgggt tccgacggac aggccgatga tgtcctcttg
     3721 ggccagggcc tcgtcgtaca gggcccggag atgctccagc gacccgtagg tgttggtgaa
     3781 cgtctggaaa taggcgatga accggccggc gtgccgggcc tcccggtagt aggcccggcc
     3841 ctttcggatc tgttcggcaa ccgccgggag cggccccgcc tgccgtaagg ccgagccgcg
     3901 gccgtcgcag tagatgcatc ccccccgcgc cacggtgccg tcgcggttgg ggcaggtgaa
     3961 cccggcgtcg atggggagct tgtagacccg gtgcccgaag gcgttcagcc agaagctctt
     4021 cagatcgtag tagcgcttgg tgttgttcca gaatgccggt ttgcccacgg ggccttcttt
     4081 ttcgttgtgt tttgaggcgg atcttattag attccccatt cgactgcaag gatttggtgc
     4141 ggggaggcgg acatttgcaa tcctatgacg tgatcgtggt cggcggaggg catgcgggct
     4201 gcgaggcggc cctcgcggcg gcccggatgg gctgcgagac cctgctgtgc aacatcaacc
     4261 tggatgccat ggccctgatg cc
//
LOCUS       DAOOLJ010000105         4020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_26996_length_4020_cov_20.264565, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000105 DAOOLJ010000000
VERSION     DAOOLJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26996_length_4020_cov_20.264565"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..864
                     /locus_tag="PLW16_10055"
     CDS             91..864
                     /locus_tag="PLW16_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB71291.1"
                     /translation="MNIVACVKQVPDTEAQIKVKPDGSGIDEGGIKWVMNPYDEFGVE
                     EALKMKEKFGGDVTIVCVGPARAMETIRTALAMGADKGIHISDPALENADAFTTASAL
                     AAAIKGIPYDVILCGQRAIDDDSGQVGSVLAELLGIPQVTVVTKVDVDGQKVKVVRPI
                     EGAQLVIESSLPAVITATKGLNEPRYASLPGIMKAKKKPVDVKDAAALGISADAKAKV
                     GKFIPPPARPPGKIICADDTPEGKAAEVAKLLREEAKVI"
     gene            896..1873
                     /locus_tag="PLW16_10060"
     CDS             896..1873
                     /locus_tag="PLW16_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPB71292.1"
                     /translation="MAKGVWIVAEHRDGALRKVSFELASTARKLADQTGDEVAAVVVG
                     SGVEAMAAELGKYGVDKVFVADNAAFEPYTTDAHAAAVAKAVKDNDPAIVLLGASAQG
                     KDLAAALVGKLAAGLATDCTDVKIEDGKLVAVRPMYAGKCFGEVAFNAAPAMASLRPN
                     VFPIVETAKAGAVVKFDAGVDAGALKTKVVEVQKEASGKIDVAEANIIVSGGRGMKGP
                     EGYGILEELAGVLGAAVGASRAAVDAGWRPQSDQVGQTGKVVSPNLYIACGISGAIQH
                     LAGMSSSKYIVAINKDNEAPIFAKADYGVVDDLFKVVPALTAECKKLMA"
     assembly_gap    1881..1980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1991..>4020
                     /locus_tag="PLW16_10065"
     CDS             1991..>4020
                     /locus_tag="PLW16_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB71293.1"
                     /translation="MEHIPFSVGNEGRQVFWNAEHFELWLFLFTFVALGIFAYGIYRR
                     WQMWAAMGKPEIRWDDTKERLKLLWRDGFLQVKTFKDPYPGIMHGLIFFGFVVLIFGA
                     AFDATEFHITEPFGVAFLRGWFYLIFSFTMDLFGLAVLIGVLMALHRRYISRPDRLSY
                     RGESDSTADDAIALLLLLGIIVTGFVIESLRIHVTRPSWEVWSFAGYALSKAFAGVPA
                     ETAKAMHAWTWWIHTFIVLGFIAYIPYSRLLHMITVPANHFMQNRKPVGTIEPIRDFE
                     NAESFGVGQLEEFTWKQIFDADACTKCGRCQDGCPAYLTGKPLSPKKLTQDLKTYWLE
                     KAPAAVMAKKLADEPARWPYLGKKWPILNTVDFILDFLLAKTKGVPAGAPAEKALVGE
                     AIELHELWACTNCMYCMENCSASIEHVPKIIAMRQYKVLTEADFAPELQLTYRNMENN
                     SNPWGVGSHLRGDWAKDLGIKTLAEDPNVEYLFYVGCSGSFDDRGKKVSVAFAKILKE
                     AGVSFGILGAEESCCGDSAMRGGNEYLYQTLAQANIEVMNGYGVKKIITTCPHGYNAL
                     KKDYPHFGGNYEVYHHTEILADLLAKGRISLKKSMDGVFTYHDSCFLGRYNNIYAQPR
                     TILGAVPGMKLVEMERNLAKSFCCGAGGARMWMEEEGQRINNTRAAQAIEVKA"
BASE COUNT          750 a         1232 c         1291 g          647 t
ORIGIN      
        1 cccggcgcct tagactaaca tcaagtgctg gatgcagcgg cgggccgctt tccggcgggg
       61 ccgccggcgg cgttcgggaa ggaggaagac gtgaatattg ttgcatgcgt aaaacaggtt
      121 ccggatacgg aagcccagat caaggtcaag ccggacggat cggggatcga tgagggcggc
      181 atcaagtggg tcatgaaccc ctacgacgaa ttcggcgtcg aggaagccct caagatgaag
      241 gaaaagttcg gcggcgacgt gacgatcgtg tgcgtgggcc ccgcccgcgc gatggagacg
      301 atccgcacgg ccctggcgat gggcgccgac aaggggatcc acatcagcga tcccgccctg
      361 gagaatgcag atgccttcac gaccgcgtcc gccctggcgg cggccatcaa gggcatcccc
      421 tatgacgtca tcctgtgcgg gcagcgcgcc atcgacgacg actccggtca ggtcgggagc
      481 gtgcttgccg aactgctggg catcccccag gtcaccgtgg tcacgaaggt ggacgtggac
      541 ggccagaagg tcaaggtggt ccggcccatc gagggtgccc agctcgtgat cgaaagctcc
      601 ctgccggcag tcatcacggc caccaagggt ctcaacgaac cccgctatgc ctccttgccg
      661 gggatcatga aggcgaagaa gaagccggtc gacgtgaagg acgccgcggc cctgggcatc
      721 tccgcggatg ccaaggccaa ggtcggcaaa ttcatcccgc ctccggccag gcctcctgga
      781 aagattatct gcgccgacga tacgcccgag ggcaaggccg ccgaagtggc caagctgctc
      841 cgggaagagg cgaaggtcat ttaattttat caaccattca tggaggaata gcgaaatggc
      901 aaaaggtgta tggattgttg cagaacatag agacggcgcc ttgaggaagg tcagcttcga
      961 gctggccagc accgcccgga agctggccga ccagacgggc gacgaagtgg ccgccgtcgt
     1021 ggtggggtcc ggcgtcgagg cgatggctgc cgagctcgga aaatacggtg tggacaaggt
     1081 cttcgtagcg gacaacgccg cgttcgagcc ctacacgacg gacgcccacg cagccgccgt
     1141 ggccaaggcc gtgaaggata acgatccggc gatcgtgctt ctgggcgcgt ccgcccaggg
     1201 caaggatctg gcggcggccc tcgtcggcaa gctggcggcg gggctcgcga cggattgcac
     1261 cgacgtgaag atcgaagacg gcaagctcgt cgcggtccgg cccatgtacg cgggcaagtg
     1321 cttcggcgag gtggccttca acgccgcccc ggcgatggcg tcgctgcgtc ccaacgtgtt
     1381 cccgatcgtg gagaccgcca aggcgggcgc cgtggtgaag ttcgacgccg gcgtcgacgc
     1441 gggcgcgctc aagacgaagg tcgtcgaggt gcagaaggag gcctccggta agatcgacgt
     1501 cgccgaggcg aacatcatcg tgtccggcgg gcgcggtatg aagggccccg agggctacgg
     1561 catcctcgaa gagctggcgg gcgtgctcgg cgcggcggtc ggcgcatcgc gcgcggccgt
     1621 ggatgcgggc tggcgtcccc agtccgacca ggtcgggcag accggcaagg tcgtgtcccc
     1681 gaacctgtac atcgcctgcg gcatctccgg cgccattcag catctggccg ggatgagctc
     1741 ctcgaagtac atcgtggcga tcaacaagga caacgaggcc ccgatcttcg cgaaggccga
     1801 ctacggcgtg gtggacgacc tcttcaaggt cgtacccgcc ctgacggcgg aatgcaaaaa
     1861 attgatggcg taaacgacat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 tggaggagtt atggagcaca tcccgttttc cgtgggcaat gaaggaaggc aagtcttctg
     2041 gaatgccgag cattttgaat tgtggctctt tctgttcacc tttgtcgccc tgggcatctt
     2101 cgcctacggc atctaccgcc gctggcagat gtgggcggcc atgggcaagc ccgagatccg
     2161 gtgggacgac acgaaggagc gcctgaagct gctgtggcgc gacggcttcc tgcaggtcaa
     2221 gaccttcaag gacccctatc ccggcatcat gcacgggctg atcttcttcg gatttgtggt
     2281 cctgatcttc ggcgccgcct tcgacgcgac ggagtttcac atcacggagc ccttcggcgt
     2341 cgccttcctg cgcgggtggt tctacctgat cttctccttc acgatggacc tcttcggcct
     2401 ggccgtcctg atcggcgtgc tgatggccct ccatcgccgc tacatttccc ggcccgaccg
     2461 cctgtcctac cggggcgagt ccgacagcac ggcggatgac gccatcgcgc tcttgctgct
     2521 gctcgggatc atcgtgaccg gtttcgtcat cgagtccctc cggattcacg tcacgcggcc
     2581 ctcctgggag gtctggtctt tcgccggcta cgcgctctcg aaggccttcg ccggcgtgcc
     2641 cgcggagacg gccaaggcga tgcacgcctg gacctggtgg atccacacct tcatcgtcct
     2701 gggcttcatc gcctacatcc cctattcgcg gctgctgcac atgatcacgg ttccggcgaa
     2761 ccatttcatg cagaaccgca agcccgtcgg gacgatcgag ccgatccgcg acttcgagaa
     2821 cgccgagtcc ttcggcgtgg ggcagctcga ggagttcacc tggaaacaga tcttcgacgc
     2881 cgacgcctgc acgaagtgcg gacgctgcca ggacggctgc ccggcctatc tgaccggcaa
     2941 accgctgtcc ccgaagaagc tcacccagga cctgaagacc tactggctgg agaaggcccc
     3001 ggccgccgtg atggcgaaga agctcgccga cgagcccgcc aggtggccct acctcggcaa
     3061 gaagtggccc atcctgaaca cggtcgactt catcctcgac ttcctgctgg cgaagaccaa
     3121 gggcgtgccc gcgggcgcgc cggcggagaa ggccctggtc ggggaggcca tcgaactcca
     3181 cgagctgtgg gcctgcacga actgcatgta ctgcatggaa aactgctccg cgtccatcga
     3241 gcacgtgccg aagatcatcg ccatgcggca gtacaaggtc ctgacggagg cggacttcgc
     3301 ccccgagctc cagttgacct accggaacat ggaaaacaac tcgaacccct ggggcgtggg
     3361 ctcccacctg cggggcgact gggcgaagga cctcggcatc aagacgctgg ccgaagaccc
     3421 gaacgtggaa tacctgttct acgtcggctg ttcggggtcg ttcgacgacc gcggcaagaa
     3481 ggtttcggtc gccttcgcca agatcctcaa ggaggccggc gtcagcttcg gcatcctcgg
     3541 cgccgaggag agctgctgcg gcgactcggc gatgcggggc ggcaacgagt acctgtacca
     3601 gacgctcgcc caggccaaca tcgaggtgat gaacggctac ggggtcaaga agatcatcac
     3661 gacctgtccc cacggctaca acgcgctcaa gaaggattat ccgcacttcg gcgggaacta
     3721 cgaggtctat caccacacgg agatcctcgc ggacctgctc gcgaagggca ggatcagcct
     3781 gaagaagtcc atggacgggg tcttcaccta ccacgactcc tgcttcctcg gccgctacaa
     3841 caacatttac gcgcagcccc ggaccatcct gggcgccgtc cccggcatga agctggtgga
     3901 gatggagcgg aacctcgcga agagcttctg ctgcggcgcc ggcggggcgc ggatgtggat
     3961 ggaggaggaa ggccagcgga tcaacaacac ccgcgccgcg caggcgatcg aggtcaaggc
//
LOCUS       DAOOLJ010000106         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_27020_length_4017_cov_23.096921, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000106 DAOOLJ010000000
VERSION     DAOOLJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27020_length_4017_cov_23.096921"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="rfaD"
                     /locus_tag="PLW16_10070"
     CDS             complement(<1..799)
                     /gene="rfaD"
                     /locus_tag="PLW16_10070"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HPB71294.1"
                     /translation="MIVVTGGAGFIGSAFVWRLNLEGIDNIVIVDRLGTEEKWKNLVN
                     RRFADFLHKDEFLRLLIKDDLPFRPRAIVHMGACSSTTERDADYLLENNFHYSCRLAE
                     WALRKRCRFVYASSAATYGDGRLGFDDGDDVTPTLRPINMYGYSKQLFDLWVLRRKAE
                     ERIAGIKFFNVFGPNEHHKGDMASVVFKAFHQIRREGRVRLFKSYRAEYPDGGQKRDF
                     VYVKDAVEVMRWLLDHPDINGLFNLGTGQARTWNDLIGAVFAALGREP"
     gene            complement(811..2013)
                     /locus_tag="PLW16_10075"
     CDS             complement(811..2013)
                     /locus_tag="PLW16_10075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB71295.1"
                     /translation="MAGAELIGKEEIQEVMDVLETGVLMRYGFDAERKGIFKVRQLEE
                     EFARYCGAAYALGVTSGSSALKVALAALGIGPGDEVICPAFTFVATYEAVLEAGAIPV
                     MADIDESLNLDPADIERKLTAHTRAVIPVHMCGAAAHIDRILAVAKKHNLLVLEDNAQ
                     GCGGSFKGKKLGTFGDMGIFSFDYYKTITTGEGGLVMTDSKALYDRADWVHDHGHDHN
                     PAVSRAMEGRTILGFNYRMNELQGAIGLAQLKKLDRVIAGQRANKAAIKAMLANVKGV
                     GFRELPDADGDTATFLAFNLPAEDLAARFQKALAAGGVDTTCFKFNKWHYVPNWEHFL
                     AQATANAKQYPFKDPSYVGKASYDRKDIPQAEDLLGRTLVMGISVRMAEERLARIGET
                     IVQAARTL"
     gene            complement(2071..3228)
                     /locus_tag="PLW16_10080"
     CDS             complement(2071..3228)
                     /locus_tag="PLW16_10080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB71296.1"
                     /translation="MVKPFAFTGAKRIVFGRAAFDGLAGQVRDLKGSRPFVVVDRNLA
                     ATGIPGRLDALLKDADLKSVLFDRAEAEPRLELADAGAQAALKAKCDLVVGVGGGSAM
                     DLAKAVAVLAANRGRAADYLGLGKVPGPGLPTIMVPTTAGTGSEVTFTAVFMRKSLKK
                     KEGMNSPYLYPDVALLDPLLTLSLPPGPTATTGIDALCHAIESFTSINASPMSELLSL
                     EAIGLIAENLRTCVHDGGNVEAREQMLLGSLYAGLGLANAGVTAVHSLSYPLGGRYGI
                     PHGMANTLLLPAVMAYNLPGALEKFTIIAEAMGEIPDDLPLRESACLAIEAVETLIED
                     CGITETLGDFDVREEDFPTLADAALTVARPLANNPRKVTREDAIQIYRDAF"
     gene            complement(3301..4008)
                     /locus_tag="PLW16_10085"
     CDS             complement(3301..4008)
                     /locus_tag="PLW16_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB71297.1"
                     /translation="MAIVLNRPKYAGNVGSAARCAKNMGIGRLIVVGRRDLDEEELRM
                     RATHEAADLIDRLEHRDGLGEALGEFRYCIGTTARLGDGRGPVVSPREMATKLAALPP
                     DNAVALLFGPEDTGLTNDDLKFCHLVVNIPTAGFKSLNLSHAVMILAYEILVARWTGR
                     KAAGHRLASVAELEGMYGQVEDLLKRIGFLNPQNPGYWMMHLRRLFARTGLTAKEVKI
                     LRGICRQVDWYAENKKA"
BASE COUNT          617 a         1309 c         1354 g          737 t
ORIGIN      
        1 cgggctcccg ccccagcgcg gcgaacacag ccccgatcag gtcgttccac gtccgggcct
       61 gacccgtccc caggttgaag aggccgttga tatcggggtg gtccaaaagc cagcgcatca
      121 cctcgaccgc gtccttcacg tacacgaagt cgcgcttctg accgccgtcg gggtactcgg
      181 cccggtacga cttgaacagc cgcacccggc cctcccgccg gatctggtgg aaggccttga
      241 agacgacgct cgccatgtcc cccttgtggt gctcgttggg gccgaagacg ttgaagaact
      301 tgatccccgc gatccgttcc tccgccttgc ggcgcaggac ccacaggtcg aagagctgct
      361 tggagtagcc gtacatgttg atggggcgca gggtgggggt gacgtcgtcg ccgtcgtcga
      421 accccagtcg gccgtcgccg taggtcgcgg cggagctggc gtagacgaac cggcagcgtt
      481 tgcgcagggc ccactccgcg agccggcagc tgtagtggaa gttgttctcc agcaggtagt
      541 cggcgtcccg ctccgtggtc gaggagcagg cccccatgtg gacgatggcc cggggccgga
      601 agggcaggtc gtccttgatc agaagccgga ggaactcgtc cttgtggagg aagtcggcga
      661 aacgccggtt gacgaggttc ttccacttct cttccgtccc caggcggtcg acgatgacga
      721 tgttgtcgat tccctcgaga ttcaaccgcc agacgaaggc gcttccgatg aatccggccc
      781 cgccggtgac gacgatcatg gtgggtctcc ttacagggtt cgggccgctt gtacgatggt
      841 ttctccgatc cgggcgagcc gctcctcggc catccggacg gaaattccca tgacgagggt
      901 ccggcccagg aggtcttccg cctgggggat gtccttccgg tcgtaggacg cctttcccac
      961 ataggacggg tccttgaacg ggtactgttt cgcgttggcg gtggcctggg cgaggaagtg
     1021 ttcccagttg gggacgtagt gccacttgtt gaacttgaag caggtcgtgt ccaccccccc
     1081 ggctgccagg gccttctgga agcgcgccgc caggtcctcg gcgggcagat tgaaggcgag
     1141 gaaagtcgcc gtgtccccgt cggcgtccgg cagctcccgg aatcccacgc ccttgacgtt
     1201 tgccagcatc gccttgatag ccgccttgtt cgctcgctgg cccgcgatga cccggtcgag
     1261 cttcttcagc tgggcgaggc cgatcgcccc ctggagctcg ttcatccggt agttgaagcc
     1321 gaggatcgtc cggccctcca tcgcccggct cacggcgggg ttgtggtcgt ggccgtggtc
     1381 gtggacccag tcggcccggt cgtagagggc cttgctgtcc gtcatgacga gcccgccctc
     1441 gccggtggtg atcgttttgt agtagtcgaa gctgaagatc cccatatcgc cgaaggtccc
     1501 gagcttcttg cctttgaagc tcccgccgca gccctgggcg ttgtcctcga gaacgagcag
     1561 gttgtgcttt ttcgcgaccg ccaggatccg gtcgatgtgg gccgcggccc cgcacatgtg
     1621 cacggggatg acggcccgcg tgtgcgcggt cagcttccgc tcgatgtccg ccgggtcgag
     1681 gttgagcgat tcgtcgatgt ccgccatgac ggggattgcc cccgcctcca ggaccgcctc
     1741 gtaggtcgcg acaaacgtga aggccgggca gatcacctcg tcgccggggc cgatgcccag
     1801 ggccgcaagc gccaccttga gcgcgctcga cccggaggtc acgcccaggg cgtaggccgc
     1861 cccgcagtat cgggcgaatt cctcctccag ttgccggacc ttgaagatcc ccttccgctc
     1921 ggcgtcgaag ccgtaccgca tcaggacgcc cgtttccagg acatccatca cctcctggat
     1981 ttcttcctta ccgatcagtt ccgcaccggc catctgccgc ctccttcagg gttatgatga
     2041 tgccgtcgat tccgcattga atgaatgaag tcaaaaagcg tcccggtaga tctggatcgc
     2101 gtcctcccgc gtgaccttcc gggggttgtt ggccaggggg cgtgccacgg tcagggccgc
     2161 atctgccaag gtggggaagt cctcctcccg gacatcgaaa tccccgaggg tctccgtgat
     2221 cccgcaatcc tcgatcaggg tctccaccgc ctcgattgcc aggcacgccg actcgcgcag
     2281 gggcagatcg tcgggaatct cccccatcgc ctcggcgatg atcgtgaact tctccaaggc
     2341 gcccggcagg ttgtaggcca tgacggcggg caggaggagc gtgttcgcca tcccgtgggg
     2401 gattccgtag cggccgccca gggggtacga gagggaatgc accgccgtca cgccggcgtt
     2461 ggcgagaccc aggcccgcgt aaaggctgcc caggagcatc tgctcgcggg cctccacgtt
     2521 cccgccgtcg tggacgcagg tccgcaggtt ctcggcgatg agtccgatcg cctccaggga
     2581 gaggagttcg ctcatcggcg aggcgttgat cgaggtgaac gattcgatgg cgtggcatag
     2641 ggcgtcgatg cccgtggtcg ccgtgggtcc cggggggagg ctcagggtca gcagggggtc
     2701 caagagcgcc acgtccggat agaggtacgg gctgttcatc ccctccttct tcttgaggct
     2761 cttccgcatg aaaaccgcgg tgaaggtcac ctcgctgccc gtccccgccg tcgtggggac
     2821 catgatcgtg gggagtcctg gcccggggac cttgccgagt cccaggtaat cggccgcccg
     2881 gccgcggttg gccgcgagga ccgccaccgc cttggcgaga tccatggcgc tgccgccgcc
     2941 cacgccgacg acgagatcgc acttcgcctt gagcgcggcc tgcgcccccg cgtcggcgag
     3001 ctccagacgg ggttccgcct ccgcgcggtc gaacaggacg gatttgaggt ccgcgtcctt
     3061 gagcagggca tccagccgac ccggaatgcc ggtggcggcg agattcctgt ccacgacgac
     3121 gaacggacgg gagcctttga ggtcgcggac ctgcccggcc agtccgtcga aggccgcccg
     3181 gccgaagacg atccgtttcg cgcccgtgaa ggcaaacggt ttcaccatgg tgtcgctcct
     3241 tttttgcacg caattgcagg gatgtttaaa ggtgggtaat aagttccgac cgggggaaag
     3301 tcaagccttt ttgttttccg cgtaccagtc cacctggcgg cagatgccgc ggaggatctt
     3361 gacctccttc gcggtcaggc ccgtccgggc gaagagacgg cggagatgca tcatccagta
     3421 gccgggattc tgcggattga ggaagccgat ccgcttcagc aggtcctcga cctggccgta
     3481 catgccctcc agctccgcca cggaggcgag tcggtggccg gcggccttgc gcccggtcca
     3541 gcgggcgacg aggatctcgt aggcgaggat catgacggcg tgcgagaggt tcagggactt
     3601 gaagccggcc gtggggatgt tcacgacgag gtggcagaac ttgaggtcgt cattggtgag
     3661 gccggtgtct tccggaccga agaggagggc gaccgcgttg tccgggggaa gcgcggcgag
     3721 cttggtcgcc atctcccggg gcgaaaccac cgggccgcgg ccgtcgccca ggcgcgccgt
     3781 tgtcccgatg cagtaccgga actcgcccag cgcctccccg aggccgtccc ggtgctccag
     3841 ccggtcgatc aggtcggcgg cctcgtgggt ggcgcgcatg cgcagctcct cctcgtcgag
     3901 gtcgcggcgg cccaccacga tgaggcggcc gatccccata ttcttcgcgc accgcgccgc
     3961 ggaaccgacg ttgcccgcat acttgggccg gttcaggacg atggcgatat cggtggt
//
LOCUS       DAOOLJ010000107         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_27230_length_3994_cov_17.245240, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000107 DAOOLJ010000000
VERSION     DAOOLJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27230_length_3994_cov_17.245240"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLW16_10090"
     CDS             <1..714
                     /locus_tag="PLW16_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71298.1"
                     /translation="SPTELTWGQFCEGASKRHVRENPRTGFLVMSLDRRLWRGKAVWT
                     RAETEGDAYIRYNRKPMWRYNAYFGIHTVHFMDLVSLHPGEALPLPGIVAGALLTGLA
                     KGARKTGRSERILTPWAEGLFNRMDALKFLAFIGADGFPEIVPLLQAQAADSRRLVFA
                     PTVYRKDLRGLQPGATVAILGLTLEMENCLVRGPFAGFNRARGAPLGVIDLDWVYNSM
                     PPIPGQIYPARPLEPVTCF"
     gene            complement(830..1774)
                     /locus_tag="PLW16_10095"
     CDS             complement(830..1774)
                     /locus_tag="PLW16_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71299.1"
                     /translation="MFSDLFARRWRGLPVHGWAGLVLIAAFWFLNWSLTGLRTLWGFF
                     PLWLGYCLFVDALVCIRKGDSLLTRNAYAYAGLFAVSAPAWWLFELLNRRLQNWLYLG
                     VDGISDLAYVLFSSLSFSTVIPAVFGTAELAGTFRWIGRFRNGPRLAPTPTVLKRFLA
                     VGVASLLALLLWPRYFFPLVWLAVYLILEPFNGRFGYRSLLADTASGDWRGVFSLWTG
                     CLICGFFWEMWNILSYPKWIYHVPFVDFCRIFEMPVLGYGGYIPFSLELFALYHLITG
                     LATRGRPQDYVELTPDADRARSTVKDPGAPPASGEAVP"
     gene            complement(1909..3333)
                     /locus_tag="PLW16_10100"
     CDS             complement(1909..3333)
                     /locus_tag="PLW16_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPB71300.1"
                     /translation="MIDFTPLFCPGSVAVIGASPNIVRDRAGFIHSLSACFQGRLYPI
                     NPRHTEIEGRPCYARITEVPEAVDYALIMLPREKVAAALRDCVQAKVRFVLVFTSGFS
                     EVGETGLEAELLSILKGSDTRMIGPNCVGAHCPERGLVYYPQMMRDTPGHVGFFSQSG
                     GHALNFLIRGLSMGIGFDKVISVGNQADLKIVDFIEYFAEDPRIDIICGYIEDIKDPE
                     RFHRAVRETVLNTRKPIVLWKGGRSADGARATQSHTGALAAPVKVWDGVMAQMGVINA
                     ETQNEMSDVLLALKFGFNPRGLRACIAVAGGGSSVELTDAFNANGVSVPELSPEVQAL
                     VGTDLSRVNTSTRNPLDLGMFGFAPNVLGEAAERAARDPNIDFIAICLYPELIQIMVR
                     ELWDGFVEKMLEIFPRISKPLVVLIPQLLFRRPEVEAVRTAFVEKLDRAGILSFPDAG
                     RAARMAVKIHRYRRFMEAHPGDDR"
     gene            3616..3975
                     /locus_tag="PLW16_10105"
     CDS             3616..3975
                     /locus_tag="PLW16_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71301.1"
                     /translation="MSEDTRKKIHDEKSLEKLTVKELREIAAEIPHERAIHDMKKEEL
                     VAFIREARGIKESAPAAKKKQPVKIKMAKPALKARIRELKALQAQARESSDRGGLVRL
                     RRQITRLKKKSRHVAAA"
BASE COUNT          802 a         1233 c         1345 g          614 t
ORIGIN      
        1 tctccgacgg aactcacgtg gggccagttc tgcgaagggg cgagcaagcg gcacgtccgg
       61 gagaacccga ggaccggctt cctcgtcatg agcctggacc ggcggctctg gcggggcaag
      121 gccgtctgga cgcgggcgga aaccgaaggc gacgcgtaca tccgctacaa ccggaagccc
      181 atgtggcgtt acaacgccta cttcggcatc cacaccgtgc acttcatgga cctggtctcg
      241 ctgcacccgg gagaggcgct gccgctcccc gggatcgtcg ccggggcgct cctgacggga
      301 ctggccaagg gggcccggaa gacgggacgg tcggagcgca tcctgacgcc ctgggcggag
      361 ggactcttca accgcatgga cgcgctgaag ttcctcgcct tcatcggcgc ggacggcttc
      421 cccgagatcg tccccctgct ccaggcgcag gccgccgaca gccgccgcct cgtcttcgcg
      481 cccaccgtct accggaaaga tctgcggggc ctgcagccgg gcgcgacggt cgcgattctc
      541 gggcttaccc tggagatgga aaactgcctt gtccgggggc cgttcgcagg cttcaaccgc
      601 gcccgcggcg caccgctcgg cgtgatcgac ctggactggg tgtacaactc catgccgccc
      661 atcccgggac agatctaccc agcacggcca ctcgaacccg tcacgtgctt ctgaggcggc
      721 aaccggtaac gcgaacgatg gatcggaagg gcgccgcatg caggatgcat gcaacgtgaa
      781 cgtcgacccc gggccgcagg atcgtccggt gtaccccgca tcccggcggc tacggcacag
      841 cctccccgga tgcggggggc gcgcccgggt ccttcactgt cgaacgggcc cggtcggcgt
      901 ccggcgtcaa ctcgacgtag tcctgcgggc gcccccgggt ggcgaggccc gtgatcaggt
      961 ggtacagggc gaagagttcc agggagaagg ggatgtaccc gccgtagccg aggaccggca
     1021 tctcgaagat gcggcagaaa tcgacgaagg ggacgtggta gatccatttc ggataggaaa
     1081 ggatgttcca catctcccag aaaaagccgc agatgaggca gcccgtccag agggaaaaca
     1141 cgccccgcca gtctcccgag gccgtgtccg cgaggagcga gcggtagccg aagcggccgt
     1201 tgaagggctc caggatcagg tacacggcca gccacacgag ggggaaaaaa taccggggcc
     1261 agagcagcag ggccagcagg ctcgcgaccc cgacggcgag gaaccgcttc aagacggtgg
     1321 gcgtgggcgc cagccgggga ccgttgcgga agcgtccgat ccaacggaag gtcccggcga
     1381 gttccgccgt accgaagacg gccgggatga ccgtcgagaa gctcaggctg gagaacagga
     1441 cgtaggccag atcggagatg ccgtcgacgc ccaggtatag ccagttctgc agacggcggt
     1501 tgaggagttc gaagagccac caggccggcg cggagacggc gaagagcccc gcgtaggcgt
     1561 aggcgttgcg ggtcagaagc gagtcgccct tgcggatgca gacgagggcg tcgacgaaca
     1621 ggcagtagcc cagccagagg gggaaaaatc cccagagggt tcgcaggccc gtcagcgacc
     1681 agttgagaaa ccagaaggcc gcgatcaaca ccagtcccgc ccagccgtgg accggcagcc
     1741 ctctccatcg tcgggcgaaa agatcggaga acatgttctt catcctcctt cccccgcgga
     1801 aaagccccct tcggaggtcc ggccggggct ctgcggacgg cgcccgggga ccgtgcgcgc
     1861 gggcgatgtc ccgtccggcg agggcggcgg aggggcggag gaggtgcttc agcggtcgtc
     1921 gcccgggtgc gcctccatga atcgccggta ccggtggatc ttcacggcca ttctcgccgc
     1981 ccgccccgca tccggaaagg agagaatccc ggcacggtcg agcttttcga cgaatgccgt
     2041 ccgcacggct tcgacctccg gccggcggaa cagcaactgc gggatcagga cgaccagcgg
     2101 cttggagatg cgggggaaga tctccagcat cttttcgacg aacccgtccc acaactccct
     2161 gaccatgatc tggatcagct ccggatacag gcaaatggcg atgaagtcaa tgttcgggtc
     2221 ccgggccgcc cgttccgcgg cctccccgag aacattgggt gcaaaaccga acatgccgag
     2281 atcgagcggg ttgcgcgtgg aggtgttgac ccgggagagg tccgttccga ccagcgcctg
     2341 cacctccggc gacagttccg gaaccgagac cccgttcgcg ttgaaggcgt cggtcagttc
     2401 cacggaactt ccgccaccgg cgaccgcgat gcaggccctg agcccccggg ggttgaaccc
     2461 gaacttcagg gcgagcagga cgtcggacat ctcattttgg gtctcggcgt tgatgacgcc
     2521 catctgggcc atcacgccgt cccagacctt cacgggggcg gccagggcgc ccgtgtggct
     2581 ctgcgtggcc cgcgcgccgt cggcggaccg tccgcccttc cagaggacga tcggttttct
     2641 cgtgttcagg accgtttcgc ggaccgcccg gtggaagcgc tcggggtcct tgatgtcttc
     2701 gatgtagccg caaatgatgt cgatacgggg atcttcggcg aagtattcga tgaaatcgac
     2761 gatcttgagg tccgcctggt tgccgacgct gatgaccttg tcaaaaccga tgcccatgga
     2821 gaggccgcgg atgaggaaat tcagggcatg gccgccggac tgggaaaaga acccgacatg
     2881 gcccggggtg tcccgcatca tctgcgggta atacaccagg ccgcgctccg ggcaatgggc
     2941 cccgacgcag ttgggaccga tcatccgggt gtcgcttccc ttgaggatcg aaagcagttc
     3001 cgcctcgagc cccgtttcgc ccacttcgga aaatcccgac gtgaagacga gcacaaagcg
     3061 aaccttggct tggacgcaat ccctcagggc ggcagcgacc ttttcccgcg ggagcatgat
     3121 cagggcgtaa tcgacggcct ccggcacctc cgtgatccgg gcgtagcagg gccggccttc
     3181 gatctccgta tgccgcgggt tgatggggta gagccggccc tggaagcagg cgctcaggct
     3241 gtgaatgaag cccgcccggt cgcggacgat gttgggcgag gcgccgatca cggcgacgga
     3301 gccgggacag aacagggggg tgaaatcgat catccgggtg cacctttcga atcgtggatg
     3361 gaaagcgaga aaaccgacga ggcaatcgtc gcccccgccg gcggtggact ataggggata
     3421 ttcaaggggg tgtcaataaa aatgccggta ttttcctctt ttaaaatgca caaatattgt
     3481 tattatatgc ttgtaggggg cgggtgtcgg gcccttcccc gccggatggc gtccatgcgg
     3541 tgtaaaccgg ggcgactcca ggtcccccct gattgcctgt agatcttgaa aataaacgaa
     3601 ggaggtctat gcgtgatgtc tgaggatacc cggaagaaaa ttcatgacga aaaatccctt
     3661 gaaaagttga ccgtcaagga attgcgcgag atcgcagccg aaatccccca cgagagggcg
     3721 attcacgaca tgaaaaagga agaactggtc gccttcattc gggaagcccg gggaatcaag
     3781 gagagcgccc ccgccgcgaa aaagaaacag cccgtcaaga tcaagatggc caaaccggcc
     3841 ctgaaggcca ggattcgcga actgaaagcc cttcaggcgc aggcgcggga atcgagcgat
     3901 agaggcggcc tcgtccgact gaggaggcag atcacccgtt tgaagaagaa atcgaggcac
     3961 gttgccgcgg cctgacggtc gacgatcgga agcg
//
LOCUS       DAOOLJ010000108         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_27775_length_3937_cov_21.575219, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000108 DAOOLJ010000000
VERSION     DAOOLJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27775_length_3937_cov_21.575219"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLW16_10110"
     CDS             <1..559
                     /locus_tag="PLW16_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPB71302.1"
                     /translation="NLLPHFIQGATVRITVAKIPEEGLKYGFTRQGEWFREFGRGRED
                     LPVELGPVKVSLKARRTGDTVYLEGMIETTVTADCCRCLEATRLPMAAAFAYTGVPEE
                     ERFAEERELRPEDLEVVFYTDDTLDLDPIVYEQILLQIPLKILCREDCRGLCPHCGAN
                     LNDGPCRCPEEAVDGRFSALKKLKI"
     gene            581..766
                     /gene="rpmF"
                     /locus_tag="PLW16_10115"
     CDS             581..766
                     /gene="rpmF"
                     /locus_tag="PLW16_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB71303.1"
                     /translation="MPNPVKRHSRTRRNKRRSHDFLTAPVTSACPQCGEPKLPHRICA
                     NCGTYKGREIVPIEKLS"
     gene            798..1739
                     /gene="fabD"
                     /locus_tag="PLW16_10120"
     CDS             798..1739
                     /gene="fabD"
                     /locus_tag="PLW16_10120"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPB71304.1"
                     /translation="MIRVGIVFPGQGTQSVGMGKDLREGSAAAGRVFDEAGRLLEMDV
                     ADLCFRGPQERLSLTVFTQIAVLTVDIACWEALREAAPVTPEVMAGHSLGEYAALYAA
                     GAVTLDDVFPLVKARAEYHQRAVPVGVGAMAAIVGLTRERVDAICREASSPETVVALA
                     LQNSPNQFVVSGHTPAVGAAVEMAAAAGALQAVRLPISVPCHSRLLDPTAALLAERLR
                     TVTFKDAAVPVIPNCDPEIFYTRDRATALLTRQITSPVRWQETVERMVALGVDTVVEV
                     GPGRVLSGLSKRIHRGLKLLNVEDAASLKKTADFLNS"
     gene            1795..2031
                     /gene="acpP"
                     /locus_tag="PLW16_10125"
     CDS             1795..2031
                     /gene="acpP"
                     /locus_tag="PLW16_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB71305.1"
                     /translation="MSLEEKVIEIIMEQLDVTREECILEASFIDDLGADSLDLVELIM
                     EMEENFGIQIADEELEKIRTIGDVIDFLHSKGVK"
     gene            2067..3308
                     /gene="fabF"
                     /locus_tag="PLW16_10130"
     CDS             2067..3308
                     /gene="fabF"
                     /locus_tag="PLW16_10130"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPB71306.1"
                     /translation="MKRRVVITGLGAVTPLGCSVPETWEAVCAGRSGVAPITRFDSTL
                     FDTKIAAELKTFDPLQYVNKKEYRRLDDFILYAIAASEMAVVDAGGAITGTNAERVGV
                     IIGSAIGGLSTMEKEKETLAAAGPRRISTFAIPAVLCNLAGGHVSIRFGAKGPITCPV
                     TACAAGTNAVGEAFRLIQNGYADVMIAGGVDAAITPLGIGGFNAMRALSTRNNNPEKA
                     SRPFDRDRDGFVVGEGGGILILEEMTAALDRGARVYAEIAGYGSTCDAFHMAAPPPGH
                     EGAVRCMRAALRDAALVPEAIDYINAHGTSTPLNDVYETQAIRTVFGTHSDRLAVSST
                     KSMTGHMLGAAGGVEAVFAALAIREGIVPPTINLDHPDPDCDLDYVPHTARRQAVRAA
                     MSNTFGFGGVNAVVILKEFQP"
     gene            3305..3796
                     /gene="rpiB"
                     /locus_tag="PLW16_10135"
     CDS             3305..3796
                     /gene="rpiB"
                     /locus_tag="PLW16_10135"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPB71307.1"
                     /translation="MTETRPRLTIGADHAGFALKEALKAALVAQGYDVVDAGTSNAEP
                     ADYPDFATLVARAISRGTGERGILICGSGIGMSITANRFPGVRAALCLDAEAARLSRL
                     HNDANILVLAARRTGTEAALEITFVWLATPFEGGRHQRRLEKLRAIEREICSTSSTDL
                     EEE"
     gene            3803..>3937
                     /gene="glyA"
                     /locus_tag="PLW16_10140"
     CDS             3803..>3937
                     /gene="glyA"
                     /locus_tag="PLW16_10140"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB71308.1"
                     /translation="MSALTHVDPEIAAAIRRETERQAGKLELIASENFVSEAVLEAQG
                     C"
BASE COUNT          744 a         1288 c         1245 g          660 t
ORIGIN      
        1 taatttactt ccgcacttta ttcagggggc gaccgtgagg atcaccgtag ccaagattcc
       61 cgaagagggc ctgaagtatg ggtttacccg gcagggcgag tggttccggg agttcgggcg
      121 ggggcgcgaa gacctccccg tcgaactggg ccctgtcaag gtctccctga aggccagaag
      181 gaccggggat acggtctacc tggaaggcat gatcgagacg acggtcacgg ccgattgctg
      241 ccgctgcctc gaggcgactc gcctgcccat ggcggcggcg ttcgcttaca ccggcgtgcc
      301 cgaggaagag cgctttgcgg aggagcgcga gctccgtccg gaagatctgg aagtggtctt
      361 ctacacggac gacacccttg atctggaccc gatcgtctac gagcagatcc tcctccagat
      421 cccgctcaag atcctctgcc gtgaggattg ccgggggttg tgcccgcact gcggcgcgaa
      481 tttgaatgac gggccgtgcc gctgcccgga agaggcggtg gacggacggt tttccgcctt
      541 gaaaaaattg aagatctgac accttaagag aggatacgct atgccaaacc cagtcaaacg
      601 acattcgcgc acgagacgca acaagaggcg gtcccatgat tttctgacgg cgcccgtgac
      661 ctcggcctgt ccacagtgcg gggagcccaa gcttcctcac cggatctgcg cgaattgcgg
      721 cacttacaag ggaagggaaa tcgttcccat cgagaagctt tcctgaagcg tcgattccgc
      781 ctgaggaagg gatcatcatg atcagggtgg ggattgtctt tccgggacag ggaacccagt
      841 ccgtgggcat gggcaaagac ctccgcgagg gaagcgccgc cgcggggcgc gtgtttgacg
      901 aagcgggccg cctgctcgag atggacgtgg ccgacctgtg cttccgggga ccgcaggagc
      961 gtctgagcct gacggtcttc acccagattg ccgttctgac cgtcgacatc gcctgctggg
     1021 aggccctccg cgaagcggcc cccgtgacgc ctgaggtcat ggccggtcac agcctgggcg
     1081 agtacgccgc cctgtacgcc gcgggcgccg tcaccctgga cgacgtcttt cccctcgtca
     1141 aggcgcgcgc cgaataccac cagcgggccg tccccgtggg cgtcggcgcc atggccgcaa
     1201 tcgtcggcct cacgcgcgaa cgggtcgatg cgatctgccg cgaggcgtcg tcaccggaaa
     1261 ccgtggtcgc cctcgccctc cagaacagtc cgaaccagtt cgtcgtctcc ggccacacac
     1321 cggcggtggg cgcggccgtc gaaatggccg cggcagcggg ggccctgcag gcggtgaggc
     1381 tccccatcag cgtcccctgc cacagccggc tgctcgaccc gaccgccgcg ctgctggccg
     1441 agcgcctgcg cacggtgacc tttaaggacg ccgccgtccc ggtcatcccc aattgcgacc
     1501 cggaaatctt ttacacacgc gaccgcgcga ccgcgctgct gacccgtcag ataacctcgc
     1561 ccgtccgctg gcaggagacc gtcgagagga tggtcgccct gggggtggat accgtcgtcg
     1621 aggtcggtcc cggacgggtc ctgtccggct tgagcaaacg gatccaccgg ggactaaagc
     1681 tcctgaacgt ggaagacgcg gcgtccctga aaaaaacggc agatttttta aacagttgat
     1741 gatatggagg cgtgggaaaa gcacgccgtc atttttaacg aggagggagg actcatgtca
     1801 ctcgaagaaa aagttatcga aatcatcatg gagcagttgg atgtcacacg ggaagagtgt
     1861 atcctggagg cgtccttcat cgacgatttg ggtgcagatt ccctcgatct ggtcgagttg
     1921 atcatggaaa tggaggaaaa tttcggcatc cagatcgcgg atgaggagct cgagaagatc
     1981 cgcaccatcg gtgacgtcat cgattttctg cacagcaagg gagtaaagta accggccctt
     2041 cgccatacgc cgtcaggggg tgtccattga aaagaagggt tgtcatcacc ggcctcgggg
     2101 ccgtgacgcc gctcggctgc tccgtcccgg aaacctggga ggccgtctgt gccggacggt
     2161 cgggcgtcgc tcccatcacc cggttcgact cgaccctctt cgacacgaag attgccgctg
     2221 aactgaagac cttcgatccc ctgcaatacg tcaacaagaa agagtaccgg cgcctcgacg
     2281 atttcatcct ctatgccatc gccgcatccg aaatggccgt ggtcgacgcg ggcggggcca
     2341 tcacggggac caacgcggag cgggtcggcg tgatcatcgg ctcggccatc ggcggactct
     2401 ccaccatgga gaaggagaag gaaaccctcg cggcggccgg cccccggcgc atttccacct
     2461 ttgccattcc ggcagtcctc tgcaatctgg cgggaggcca cgtctctatc cgcttcggcg
     2521 ccaagggacc gatcacctgc cccgtgaccg cgtgcgccgc gggcacgaac gccgtcgggg
     2581 aagccttccg tcttatccag aacggatacg ccgacgtaat gatcgccggc ggggtggatg
     2641 ccgccatcac ccccctcgga atcggcgggt tcaacgccat gcgggcgctc tcgactcgga
     2701 acaacaatcc ggaaaaggcc agccggccgt tcgaccgaga ccgcgacggc ttcgttgtcg
     2761 gtgaaggcgg cggcatcctc atcctggagg aaatgaccgc ggccctggac cggggcgcca
     2821 gggtctacgc ggaaatagcg ggctatggct ccacgtgcga cgcctttcac atggcggcgc
     2881 cgccgccggg acatgagggg gccgtccggt gcatgcgggc cgcgctccgg gacgccgcac
     2941 ttgtccccga ggccatcgat tacatcaacg cccacggaac ctccacccca ctgaacgacg
     3001 tctatgaaac ccaggccatc cgcaccgttt tcggcacgca cagcgaccgg cttgccgtca
     3061 gctccaccaa gtccatgacg gggcacatgc tgggcgccgc cggcggcgtg gaggccgtgt
     3121 tcgccgcact ggccatccgg gaaggcatcg tccccccgac gatcaacctc gaccatcccg
     3181 acccggactg cgacctcgac tatgtccccc acacggcccg ccggcaggcc gtccgcgccg
     3241 ccatgtccaa caccttcggc ttcggcggcg tcaacgccgt agtgatcttg aaggaattcc
     3301 agccatgacc gaaacccgcc ccagactcac catcggcgcc gatcacgcgg gctttgccct
     3361 gaaggaggcc ctcaaagccg ccctcgtcgc gcaagggtac gacgtcgtgg atgcgggaac
     3421 ctcgaacgcg gaaccggcgg actaccccga tttcgccacc ctcgtcgcgc gcgcgatctc
     3481 ccggggaacc ggcgagaggg gcatcctgat ctgcggttcg ggcatcggga tgagcatcac
     3541 ggccaaccgt ttcccgggag tccgcgcggc actctgcctg gatgccgagg cggcacggct
     3601 gagccgcctc cacaacgacg ccaatatcct ggtactcgcg gctcgccgga ccggaaccga
     3661 ggccgccctc gaaattacgt tcgtctggct ggcgacgcct ttcgagggcg gccggcatca
     3721 gcggcgccta gagaagctcc gggccatcga acgagagatt tgttcaacat catctaccga
     3781 tcttgaggag gaataaccgt acatgtctgc tttgacccac gttgatcccg aaatcgccgc
     3841 cgccatccgc cgggaaaccg agcggcaggc gggcaagctg gagctcatcg cttcggaaaa
     3901 cttcgtcagc gaggcggtcc tggaggccca gggctgc
//
LOCUS       DAOOLJ010000109         3790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_29237_length_3790_cov_9.201071, whole genome shotgun sequence.
ACCESSION   DAOOLJ010000109 DAOOLJ010000000
VERSION     DAOOLJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29237_length_3790_cov_9.201071"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLW16_10145"
     CDS             <1..714
                     /locus_tag="PLW16_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71309.1"
                     /translation="GFSTKFFVFDPYARLTFGPVYIEAEGIYITGETEWDNKAKLGAL
                     AAGLPGYVGRGGAAKKDIDLEEIAVFLHVKVDLAPVYFGVYAGYVSGDDITTADESEG
                     RNLAAGKAGSDFKPSLMLFNDDYATQFAGSTGVASLGSYMGNVIYGQLYVGFKPTPKL
                     DIRFSYAYAEADEPEVANQDEEYGSEIDVTASYKIYDNLEYMVGGAYFMTGDFFKRGV
                     AAVEVEDDYMLMHKLTLKF"
     gene            985..1251
                     /locus_tag="PLW16_10150"
     CDS             985..1251
                     /locus_tag="PLW16_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPB71310.1"
                     /translation="MQNHTISIAEGKKAFSRLIQDVADHKGDVVVTRRGKPVAVVIPH
                     DEYRKLARGEAYRKIMAARERFCRSELKASDIYEESRQELKDRS"
     gene            1248..1679
                     /locus_tag="PLW16_10155"
     CDS             1248..1679
                     /locus_tag="PLW16_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPB71311.1"
                     /translation="MRTVVCDASVVLKWYLPDEGLGAQAIGILERYLADEVDIIAPAL
                     LEYEIINALIMAARRGRIEDAAIHEALEGFSGLGISLLPVTDLYRDICEFCRKHGHTA
                     YDASYLALAVARGADLITADQRFYNSVKISMPCVHWLGNAD"
     gene            complement(1786..3321)
                     /locus_tag="PLW16_10160"
     CDS             complement(1786..3321)
                     /locus_tag="PLW16_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB71312.1"
                     /translation="MTTKLCVFVSSVQKELEDERLIVQNLMNTDVFLSAYCTPVLYEL
                     EPASPDKALEGCLQSLDGCQVYLLIVGVEYGTPVGKLSITHAEYRRAKERKLPILAFI
                     KGDRSVKREEGTAKLLAELDADGPKYKRFGNVIELQKEVRAALVKLLKDRFGISPTSD
                     ENQIAEQTIEATSPFESQPLARLRWQDLDQEVLRRLITTAENRDAGSLSDADLAAGAA
                     ARGLVWRDLGSGQIYATAAGIVLLARDPSMVFPQCRILADAYRSTEPDGDPRDHEDIR
                     SPMPLAIDRAIAFIDRNTRHPMRIVGLNRVRLDEYPVDGLREALVNAVAHRLYEDAGR
                     KILLEIFADRVVLSSPGLPPKPITLASLRKGKYRPCSRNPVVAQCLSYFHRIEERGSG
                     FRRMRDQMLDHGLEPPILGTDMGYFQVTFQGPSDNIERLRVPEKRLLVTPSMEAQLNE
                     RQKAMVAILVRGEALTSRRCEEEFHVTRDTANRDFVLLVKLGLAVKEGKGRSTRYVLP
                     AKT"
BASE COUNT          798 a         1113 c         1117 g          762 t
ORIGIN      
        1 ggcttctcga cgaagttctt cgttttcgac ccctatgccc ggctcacttt cgggccggtc
       61 tacatcgagg ccgagggaat ttacatcacc ggcgaaaccg agtgggacaa caaggcgaaa
      121 ttgggcgccc tcgcggccgg gctcccgggt tacgtgggac gcggcggagc ggccaagaag
      181 gatatcgatc tcgaggaaat cgccgtcttc ctgcatgtca aggtcgacct cgcccccgtg
      241 tacttcggcg tctatgcggg ctacgtctct ggcgacgaca tcaccacggc ggacgagtcc
      301 gagggcagga atctggcggc tgggaaggcc gggagcgact tcaaacccag cctgatgctc
      361 ttcaacgacg actacgccac gcagttcgcc ggatcgacgg gcgtggcctc gctcggcagc
      421 tacatgggca acgtcatcta cggtcagctc tatgtcggct tcaagcccac gccgaaactg
      481 gacatccggt tcagctacgc ctacgcggaa gcggacgagc ccgaggtcgc caaccaggac
      541 gaggagtacg gctccgaaat cgacgtgacc gcgagctaca agatctacga caacctcgag
      601 tacatggtgg gcggcgccta cttcatgacg ggcgacttct tcaagcgcgg cgtggcggcg
      661 gtcgaagtcg aagacgacta catgctcatg cacaagctga ccctgaagtt ctaaggggaa
      721 agcgtggggt tgcaacggag aatcaacacc taacctaacc gccgcctgcc aaggcggcca
      781 cagacgaacg ccccggcggc catccggtct ccggggcgtt tttttgcctg cacgggcatg
      841 acccatcacc gctcgagcca tgcccttcta tccgtcaggt gggatttcaa gacgcgcccc
      901 gccgatggca gcccctgttg atggttgaca tccattttgt atccatttat aatggtcaca
      961 tctgatagtc acatggaggg cctcgtgcaa aaccatacga tcagcatcgc ggaagggaaa
     1021 aaggcgttca gccgcctgat tcaggatgtg gcggatcata agggggacgt ggtcgtgacg
     1081 cgccggggaa aacctgtcgc agtggtcata ccccatgacg agtatcggaa gctcgcccgt
     1141 ggtgaagcct atcggaagat catggccgcg cgtgagcgct tttgccggag cgagctgaag
     1201 gcgtccgaca tctatgaaga gtccagacag gaactgaagg atagatcatg agaaccgttg
     1261 tctgcgatgc gagcgtcgtt ctcaagtggt atctacccga cgagggtctg ggcgcgcagg
     1321 ccatcgggat tctggagcga tacctcgcgg acgaggtgga catcatcgcg cccgcacttc
     1381 tggaatatga aatcatcaat gccctgatta tggccgcgcg acgcggcagg atcgaggacg
     1441 cggcgattca tgaggccctg gagggatttt ccgggctggg gatcagcttg ctccccgtca
     1501 ccgaccttta tcgggatatc tgcgaattct gtcggaaaca tggccatacg gcgtatgatg
     1561 ccagctatct ggccctggcc gtcgcccgcg gcgccgacct gatcaccgca gatcaacgtt
     1621 tctataacag cgtcaagatc tcgatgccct gcgtccactg gctggggaat gcggattgat
     1681 gagcggacgg cgccgttatc ggatgagaat ctgacgatta gcgccgagaa agccggcatc
     1741 actcacctga cgattagaca atcttgcctg acgattacct gacgattatg tctttgcagg
     1801 aagcacatac cgggtcgatc gccctttgcc ttctttaacc gccaagccca acttcaccaa
     1861 tagcacaaaa tccctattcg ccgtgtcacg ggtgacgtga aattcctctt cgcaacggcg
     1921 acttgtcagt gcctctcctc gaaccaggat tgccaccatg gccttctgcc gctcattcaa
     1981 ctgggcctcc atggaagggg tgaccaagag ccgcttttcg ggcacccgca ggcgttcgat
     2041 attatcgctg gggccctgga aggtaacttg gaagtatccc atgtcggttc cgagaatcgg
     2101 cggctccagc ccgtggtcca gcatctggtc acgcatccgc cggaacccgc tgccccgctc
     2161 ctcgatgcgg tggaaatagg aaaggcactg cgccacgacc ggattacggg aacagggccg
     2221 atacttcccc ttccgcaggc ttgccagtgt tatcggtttg gggggaaggc ccgggctgga
     2281 gagcacgacg cgatcggcaa aaatctcaag gaggatcttg cggccggcat cctcgtatag
     2341 ccggtgggcc acggcgttga ccagggcctc gcgcaggccg tcgacgggat actcgtcgag
     2401 gcgcacccgg ttgaggccga cgatgcgcat cgggtggcgt gtgttgcggt cgatgaaggc
     2461 aatggcacgg tctatggcca gcggcatggg tgaccggata tcctcatggt cccgtggatc
     2521 gccgtccggt tccgtactcc ggtaggcgtc agccagtatc cggcactggg ggaagaccat
     2581 cgaaggatcc ctcgcgagga ggacgatgcc ggcagccgtg gcgtagattt gccctgaccc
     2641 caagtcgcgc cagaccagac cgcgagcggc ggcaccggct gccaaatcgg catctgaaag
     2701 actgcccgcg tcccgattct cggccgtggt aatcagcctc ctgagcactt cctggtccaa
     2761 atcctgccac cggagacggg caagaggctg cgactcgaac ggggaagtcg cctcgatggt
     2821 ctgttcggcg atctggtttt catcgctggt cggcgaaatt ccgaaccgat ccttcaacag
     2881 tttcaccagg gccgcgcgga cctccttctg aagttcgatc acgttgccga aacgtttgta
     2941 cttcggcccg tccgcatcga gttcggcaag gagctttgcc gttccctctt cacgcttcac
     3001 gctgcggtcg cctttgatga acgcaagaat ggggagtttc cgctcctttg cgcgacgata
     3061 ctcggcatgg gtgatcgata gtttccccac cggcgtgccg tattcgacgc cgacgatcag
     3121 caaatagact tggcaaccgt cgagcgattg cagacatccc tccagcgcct tgtccggaga
     3181 ggccggttcc agttcataaa gcacaggcgt gcaataagcc gagagaaaca catctgtgtt
     3241 catcaagttc tgaacgatca ggcgctcgtc ttccagttcc ttttggactg agctgacaaa
     3301 aacgcaaagt ttcgtggtca ttgtggcgcc tccgcccacg aaagcagttc ggcaaaatca
     3361 tagttgacgt tcttcacacc gaagaccggc ttgcgttgga aaggcggccg gatataatag
     3421 acgcgatgcg agtctccggc gataaaatcg acgatgtcga ggattgaatc taaccaaatg
     3481 cagagtatat gtcaaaccac gatcttatcg ggctcgattt tttcaggcgc tatgtcctaa
     3541 tcatcctaac aatcggaaat aacgagcatg gcctatccgt tcacccggaa tgaagtcagg
     3601 gccacccagg acgtggatgt catctttcac ctgaccgggc gagccaggcg cgcctgccgc
     3661 tgctcatcga gcgactgcga gaaatagcag agatgtccga ctagaaatca tcatcgccgc
     3721 catcgggaag tttgatttat tccgccccgt tcgaggcctc cttctccagg taggcgaagt
     3781 cgatctggtc
//
LOCUS       DAOOLJ010000110         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_32607_length_3491_cov_19.365250, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000110 DAOOLJ010000000
VERSION     DAOOLJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32607_length_3491_cov_19.365250"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLW16_10165"
     CDS             <1..293
                     /locus_tag="PLW16_10165"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPB71313.1"
                     /translation="DHLGVDLFEYHDRTGYIFPIIRTAVKHIQSLRRGDEFVCRATVK
                     EARAKIVVAFEIRRFSDGTVCARGTTEQVAVRTPEMEMVFTIPDEISRALGC"
     gene            295..2013
                     /locus_tag="PLW16_10170"
     CDS             295..2013
                     /locus_tag="PLW16_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB71314.1"
                     /translation="METSFSQALIAGVERVSAWADLLDDINVFPVADGDTGRNLVISL
                     SPLRQITQGRDRIIRELLLSARGNSGNIAARFFSGLLMADAMHNLPDAIREGRNRAWQ
                     AVNDPKPGTMLTVFDALVAALEEAPGTYDAAWRERVLDRLEGAVRETPELLPKLKAAG
                     VVDAGALGVFIYLDGFFSALTGNIDSFKPITDIFRDSLKISSEFQEAGEGGYCVDTVL
                     KGAADDADTIRRLAAIGESVVVLKDGDYLKVHLHARDGQKARGELGAFGDVVRWFVDD
                     LDAQVHSFRRRRVEAAVHILTDAAGSVNRQNTAQLGITLLDSYLTVGDKCLPETHFAP
                     EELYDAMRRGVKVSTSQASVFERHQYYQSVLERYPRVLYLCVGSVFTGNYSVAMDWKK
                     THDPEDRLTVIDTQAASGRLGLMSLAAARFSLKTRDAEAVIRYARENVDRCEEYVFLD
                     KLQYLAAGGRLSKTSAFFGDMLRMKPVISPLAEGAKKMGVVRTQADQVDFAQEKLAAA
                     LSPEMPALIMLEYSDNRTWVAESVQPEIARRYPAAEIVLHPLSLTSGAHMGPGTWAVA
                     FLPAGE"
     gene            2091..3323
                     /locus_tag="PLW16_10175"
     CDS             2091..3323
                     /locus_tag="PLW16_10175"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HPB71315.1"
                     /translation="MPDRRRVVVTGIGLRTPIGHSLAEMAEAFRQGRTGVRAMPEWRK
                     IDHLRTAVAGVCEGVDETAVPRHFRRAMGRVAVLAALSATDALVDAGIGEERIASSRC
                     GVSFGSTAGSSQAMEDYLRQIFASRSLKGLQSSVYIRFMSHTCAANLATMFQVQGPVI
                     ASCTACVAGSQGIGYGYEAIRSGRADVMIAGGAEEMHYMDAGIFDIMRATSTRYNDRP
                     DRTPRPFDRARDGLVVGEGGGCLVLEAYEHARSRGAAIHAEIIGFGNNCDGGHITNPS
                     AEGMAAAMRLALEDAGIGAEDVGHVHAHATATEQGDIAESLATHAVFGEGVPVSAIKG
                     YMGHTLGACGAIESAVTILMLKEGFVVPTRNLDEPDPACAPLNHVRGACRPHRFRIGM
                     NNNFAFGGINTSLLFARV"
BASE COUNT          576 a         1182 c         1184 g          549 t
ORIGIN      
        1 tcgatcacct gggcgtcgac ctgttcgaat atcacgaccg gaccggctac atcttcccca
       61 tcatccgcac ggcggtcaag cacatccagt cgctccggcg gggagacgag ttcgtctgcc
      121 gtgccaccgt caaggaggcc cgtgccaaga tcgtcgtggc cttcgagatc cggcgcttca
      181 gcgacgggac cgtctgcgcc cgggggacaa cggagcaggt cgccgtccga acgccagaga
      241 tggaaatggt ctttacgatc ccggatgaaa tcagccgggc cttaggatgc tgacatggaa
      301 acctcttttt cccaagccct gatcgccggc gtggagcgcg tctccgcgtg ggccgacctg
      361 ttggacgaca tcaacgtctt cccggtggcc gatggcgata cgggacgcaa cctcgtcatc
      421 agtctgtccc ccctgcgcca gatcacgcag ggacgggacc ggatcatccg cgaactgctc
      481 ctgtcggccc ggggcaattc cggaaacatc gcggcccgat tcttctccgg cctgttgatg
      541 gcggatgcga tgcacaacct gcccgacgcc atccgcgagg gtcggaaccg ggcctggcag
      601 gcggtcaacg accccaagcc gggcacgatg ctcaccgtgt ttgacgccct ggtcgcggcc
      661 ttggaggagg cgcccgggac gtacgacgcc gcctggcgtg aacgggtgct cgaccgcctc
      721 gaaggggccg tccgcgagac ccccgaactg ctgccgaagc tcaaggcggc gggcgtcgtc
      781 gacgccgggg ccctgggcgt cttcatttac ctggacgggt tcttcagcgc gcttacgggg
      841 aacatcgatt ccttcaagcc gatcaccgac attttcaggg actcgctcaa gatctcttcg
      901 gaattccagg aggccgggga ggggggctat tgcgtggaca cggtgctcaa gggcgcagcg
      961 gacgacgcgg acaccatccg gcggctggcg gccatcggcg agagcgtcgt cgtcctgaag
     1021 gacggggatt acctgaaggt ccatctgcac gcgcgggacg ggcagaaggc ccgcggggaa
     1081 ctcggcgcct tcggggacgt ggtccgctgg ttcgtcgacg acctggacgc ccaggtccat
     1141 tccttccggc gccggcgcgt cgaggcggcc gtccacatcc tgacggacgc cgcgggctcc
     1201 gtgaaccgcc agaacacggc gcaactgggg atcaccctgc tcgacagcta tctcacggtc
     1261 ggggacaaat gcctgcccga gacccacttc gcgccggagg agctctacga cgcgatgcgc
     1321 cgcggggtca aggtctccac ctcccaggcc tccgtcttcg agcgccacca gtattaccag
     1381 agcgtcctgg aacgctaccc acgggtcttg tacctgtgcg tcggctcggt gttcaccgga
     1441 aattacagcg ttgcgatgga ctggaaaaag acccacgatc cggaggaccg cctgacggtg
     1501 atcgacacgc aggcggcctc ggggcggctg gggttgatga gcctggccgc cgcccggttc
     1561 tccctgaaga cccgggacgc ggaagcggtg atccggtacg cccgcgaaaa cgtcgatcgc
     1621 tgcgaggagt acgtctttct cgataagctc cagtacctgg ccgccggggg ccgcctgtcc
     1681 aagacgagcg ccttcttcgg cgatatgctg cgcatgaagc cggtgatcag cccgctcgca
     1741 gagggggcca agaagatggg ggtcgtccgg acccaggccg accaggtgga cttcgcccag
     1801 gagaagctgg ccgccgcgct ttcgcccgag atgccggccc tgatcatgct cgaatattcc
     1861 gacaaccgga cctgggtcgc cgaatcggtg cagccggaga ttgcccgccg ctacccggct
     1921 gcggagatcg ttctccaccc cctgtcgctc acgtccgggg cccacatggg accgggcacg
     1981 tgggccgtgg ccttcctacc cgccggggaa tgaaaggcgt tgccgcggga cgtcatggag
     2041 gacagtaggg gtctcaggtc atcacacggg accgaaagga cggatgaatc atgccggatc
     2101 ggcgccgcgt cgttgtcacg gggatcggtc ttcggacgcc gatcggtcac tccctcgcgg
     2161 agatggccga ggccttccgg caggggcgga ccggggtccg ggccatgccg gagtggcgca
     2221 agatcgatca tctccgcacg gccgtcgccg gcgtctgcga gggggtggac gaaacggcgg
     2281 tcccccggca tttccgccgc gccatgggcc gggtcgcggt cctggcggcc ctgagcgcca
     2341 ccgatgcgct ggtggacgcc ggcatcgggg aagaacggat cgcctcgtcc cgctgcgggg
     2401 tgtccttcgg ctccaccgcc ggcagctccc aggccatgga ggactacctc cgccagatct
     2461 tcgcgtcgcg gagcctgaaa ggtctccagt cctccgtcta catccgcttc atgagccaca
     2521 cctgcgcggc caacctggcg acgatgttcc aggtccaggg gccggtcatc gcctcctgca
     2581 cggcctgcgt ggccgggtcc caggggatcg gttacgggta cgaggccatc cggtcgggcc
     2641 gggcggacgt catgatcgcc ggcggcgcag aggagatgca ctacatggac gccgggatct
     2701 tcgacatcat gcgcgccacc tccacgcgct acaacgatcg accggaccgg acgccccggc
     2761 ccttcgaccg ggcgcgcgac gggctcgtcg tgggggaggg cggcggctgc ctcgtcctcg
     2821 aggcgtacga gcacgccaga agccgcggcg cggccatcca cgccgagatc atcgggttcg
     2881 gaaacaactg tgacggcggc catatcacca atcccagcgc cgaggggatg gcggcggcga
     2941 tgcgcctcgc cctcgaagac gccggcatcg gcgcggagga cgtgggccat gtgcacgccc
     3001 atgccaccgc gacggagcag ggggacatcg ccgagtccct cgcgacgcac gccgtcttcg
     3061 gggagggggt gccggtgagc gcgatcaagg gctacatggg gcataccctg ggagcgtgcg
     3121 gcgccatcga atccgccgtc acgatcctga tgctcaagga aggcttcgtc gtgccgacgc
     3181 ggaacctgga cgaacccgat ccggcctgtg cgccgctcaa tcatgtccgc ggcgcgtgcc
     3241 ggccccatcg cttccggatc ggcatgaaca acaactttgc cttcggcggg atcaatacct
     3301 ccctgctgtt tgcccgggtc tgacgggggc gaccgtggag acaagccccg acggacgctt
     3361 taccatcctc atcccggctt acaaccatgc ggcgcaaatc gccgccgtcg tcgcccgcgc
     3421 ccgctccctg gggctgccgg tctgcgtgat cgacgacggc tcgacggacg cgacggccgc
     3481 gcgcctggag g
//
LOCUS       DAOOLJ010000111         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_37460_length_3149_cov_13.970588, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000111 DAOOLJ010000000
VERSION     DAOOLJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37460_length_3149_cov_13.970588"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="PLW16_10180"
     CDS             <1..2008
                     /locus_tag="PLW16_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB71316.1"
                     /translation="IDRWTYFFFVFSIPVFIQFVHGFLGLRRRGWMVALAYAAAFLFL
                     PFVHTPSFISGFHTYSFGTIAKAGPVYHVFSGVVGLAMTYCLLVLANAVKRTQDPAQR
                     NRIRYILFGMGFSALLISLNILPVSGIDIYPLGNFSFIPAIVLAFGVLKYDLLDIGVL
                     IRKGTIYFLLTGVLTLVYIAAIYLFHLVFIDSAGGRSILLPMVLALIIVLLFNPLKGR
                     IQDLIDHVFFRAKYDYQELLRTVSGELAGMLKHEQIVRLLLTTMVDALQVTQAGAAVL
                     RPDGERLALSVHDGRHFRRGEEISITPEHPLIQWMTRTGKPVVRSQAHRLDLPAVEQK
                     RVESVMQFLDAVILIPLLSGKRLIGFFFLSEKRSGELFVHEDLELLTTLANQSVTAIE
                     NAQTYEALEELNRELEKKVEARTLALKKALEERERTQQQLIQSESLAAIGQLVAGTAH
                     ELNNPLAGATSLIQSSLESIQQDLTPSEKDELIDDMKFVLKELQRAAAIVRSLLGLSR
                     QTQTYMEPVNMNLILDDALRILYNQYKHLEVEIIKHYQEDLPEINGNFANLGQVFINI
                     LMNALQSLPEGKGSLVLETGCRAERDVVWISCRDSGQGIPPDRIKDIFKPFFTTKEVG
                     KGTGLGLYISHEIIQRHGGSITAQSVPGEGTTMTVTLPRRGRMG"
     gene            2005..3036
                     /locus_tag="PLW16_10185"
     CDS             2005..3036
                     /locus_tag="PLW16_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB71317.1"
                     /translation="MIGLMIVDDEEGVRRSVKKVLQKDGYRIFTAERGEEAVELVRLH
                     AQEIGTVISDYKMPGIDGLETLVQIGRLNSEITRIMLTGYATMESAIEAVNAGIDGFL
                     TKPFENAELRAKVREYNVKKRLKQFVSEPVLEALQRDMGSMAPRKCTVSVLFTDIRGF
                     SALTERLSPMELSELLNRKYFSPFDDIIFLYNGTLDKHIGDGIMGIYGAPISYGNDVE
                     RAILTALSMKEEMRRINDGLAESGIRIEVGYGIATGEVMAGIFGSQRKKEYTALGPTV
                     NIAARLEGLAGVDQILVCEQTYERVRDIVEAEKIDTRALKGMTRKIDIYAINGHRDRN
                     SLTSSGASV"
     gene            3067..3143
                     /locus_tag="PLW16_10190"
     tRNA            3067..3143
                     /locus_tag="PLW16_10190"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3101..3103,aa:Met,seq:cat)
BASE COUNT          704 a          843 c          915 g          687 t
ORIGIN      
        1 gatcgatcgc tggacctatt ttttcttcgt tttcagcata cccgttttca tacagttcgt
       61 gcatggcttt ctcggcctca ggcgtagggg gtggatggtc gccttggctt acgcggctgc
      121 cttcctgttt ctgccgtttg tccatacacc ctccttcatc agcggcttcc atacctactc
      181 gtttggaacg attgcgaagg cgggtcccgt ttaccatgta ttttcggggg ttgtcggcct
      241 ggcgatgacc tactgccttc tggtcttggc gaatgccgta aagaggacac aggaccccgc
      301 gcaacgcaac cggatccgct atatcctgtt cggcatgggt ttcagcgcgc tgctgatatc
      361 gctcaacatc ctccccgtca gcggaatcga catttacccg ctcgggaact tcagcttcat
      421 tcctgccatt gttctggcgt ttggcgtttt gaaatacgat cttctcgata tcggcgttct
      481 gatccggaag ggaacgatct actttcttct caccggtgtc ctgacgctcg tctatatcgc
      541 cgccatctat ctttttcatc tggttttcat cgatagcgcg ggcgggcgat ccatcctcct
      601 gccgatggtc ctggccttga tcatcgtcct gctcttcaat ccactcaagg gacgcattca
      661 ggatctgatc gatcacgtct tcttccgtgc caaatatgat taccaggaac tgctgcgcac
      721 ggtcagcgga gaacttgcag gaatgttgaa acacgagcag atcgtgcggc ttctgctcac
      781 gacgatggtc gatgcgctcc aggtcacgca agccggagcg gccgttttgc gtccggacgg
      841 agaacggttg gccctgtcag tccacgatgg tcgccatttc agaagagggg aggaaatttc
      901 catcaccccc gagcatccgc tgattcagtg gatgacacgg accggcaagc ccgtcgtgcg
      961 gtctcaggcc catcggctgg acctgcccgc cgtcgaacag aagcgggtcg aatccgtcat
     1021 gcaattcctc gatgcagtga tcctcattcc gctgttgtcc gggaaacgac tgatcggatt
     1081 tttcttcttg agcgaaaagc gctcagggga gctgtttgtc catgaggatc tggaattgct
     1141 gaccacgctg gcgaatcaaa gcgtcaccgc catcgaaaac gcgcagacct acgaggccct
     1201 ggaggaactg aaccgcgaac tcgagaaaaa agtcgaggcg cgaacgctgg ctctcaagaa
     1261 ggccctggaa gagcgggaac gaacacagca gcagttgatt cagtcggaaa gcctggccgc
     1321 gatcggccaa ctggtggcgg gtacggccca tgagctgaac aatccgctgg cgggcgccac
     1381 cagcctgatt cagtcttccc tggaatcgat ccagcaggac ctgacgccgt cggaaaaaga
     1441 cgaactgatc gacgacatga agtttgtcct caaggagctt cagcgcgccg ccgccatcgt
     1501 ccgcagcctt ctcgggctct cccgccagac gcaaacctac atggaaccgg taaacatgaa
     1561 tctcattctc gacgatgccc tgcgcatcct gtataatcaa tacaaacatc tggaagtgga
     1621 gatcatcaag cactatcagg aggatctgcc tgagattaac gggaactttg ccaacctggg
     1681 tcaggtcttc atcaatatcc tcatgaatgc cttacagtca ctgcccgaag gcaaggggag
     1741 cctcgttttg gaaacggggt gccgggccga acgggatgtc gtgtggattt cctgccggga
     1801 ttccgggcag gggattccac cggaccggat caaagacatc ttcaagcctt tttttaccac
     1861 caaggaagtc gggaagggaa ccggcctggg gctctacatt tctcatgaaa tcatccagag
     1921 acatgggggg agcatcacgg cgcaaagtgt tccgggcgag ggaacgacga tgaccgttac
     1981 cctgcccaga agagggagga tgggatgatc ggcctgatga ttgtggacga cgaagaaggg
     2041 gtgcggaggt ccgtcaagaa ggtcctccaa aaagacggtt accggatttt tacggcggaa
     2101 cggggagaag aggccgtcga actggttcgc cttcatgcac aggagatcgg gaccgtgatc
     2161 tcggattaca agatgcccgg catcgatggc ttggaaaccc tggttcaaat cggccggctc
     2221 aattccgaaa tcacccggat catgctgacc ggctatgcga ccatggaaag tgcgatcgag
     2281 gccgtcaatg ccgggatcga cggttttctg accaagccgt ttgaaaacgc ggaactgcgg
     2341 gcgaaagtgc gtgagtacaa tgtcaagaag cgcctgaagc agttcgtgtc cgagcccgtt
     2401 ctcgaagcgc tgcagcggga tatggggagt atggcaccgc gaaagtgcac ggtcagcgtc
     2461 ctgtttacgg atattcgagg tttctcagcg ttgacggaac gtctgagccc catggagcta
     2521 tccgaactgc tgaaccggaa atatttttct cccttcgacg atatcatctt cctttacaat
     2581 gggacgttgg acaagcacat cggcgatggg atcatgggga tttacggggc cccgatcagt
     2641 tatggcaatg atgtggaacg cgcgatctta accgccctga gcatgaaaga ggagatgcgg
     2701 cggatcaatg acggcctcgc ggaatccggg atcaggatcg aggtggggta cgggatcgcg
     2761 accggagagg tcatggcggg tatcttcggc tcacagcgaa aaaaggagta tacggcgctt
     2821 ggaccgacgg tgaatatcgc ggctcggctg gaaggcctgg ccggcgtcga tcagattttg
     2881 gtttgcgaac agacttacga gcgggtacgg gacattgtcg aagccgagaa gatcgatacc
     2941 cgggccctca aggggatgac gagaaaaatc gatatctacg cgattaacgg gcacagggat
     3001 agaaactcct tgacaagcag cggcgcttcg gtttaaaaga cactttcatt tttgagatcg
     3061 gcgtgacgcg gggtggagca gtccggtagc tcgttgggct cataacccaa aggtcagagg
     3121 ttcgaatcct ctccccgcta ccaaatcaa
//
LOCUS       DAOOLJ010000112         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_42014_length_2895_cov_14.230634, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000112 DAOOLJ010000000
VERSION     DAOOLJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42014_length_2895_cov_14.230634"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLW16_10195"
     CDS             <1..863
                     /locus_tag="PLW16_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPB71318.1"
                     /translation="TFAAEVKAEADALTVNGKRIPITTQTGALNELPWKSLGVDIVME
                     CTGKFRKRDELAGHLAAGARKVLLAVPGKGLDATFVMGVNEETYDPARHDIVSNASCT
                     TNCMAPVAKVIHDAFGIVKGLMTTVHAYTMDQRLLDGSHKDLRRGRAAALSIVPTSTG
                     AASAVAEVIPELEGKLGGVALRVPTPNVSLVDLAVEVGRNVTIAEVNGALKAAAEGRL
                     KGIMAFCEEELVSIDFTSSPYSAIVDAPLTGVINGNFVKVFAWYDNESGYACRMRDLA
                     LHIAARSFGG"
     gene            865..1617
                     /gene="tpiA"
                     /locus_tag="PLW16_10200"
     CDS             865..1617
                     /gene="tpiA"
                     /locus_tag="PLW16_10200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB71319.1"
                     /translation="MIRPLIAGNWKMHKTVREAVAFAGQLREALPDLQDREVVVAPPF
                     PALVPVAEALRGSAIALGAQNLHDAAQGAFTGEVSAAMLVDAGCRFVIVGHSERRALF
                     GETDASINHKLAQAVTNGLVPIFCIGETLPEREADRTLAVVARQLKEGLNNLSSNDMK
                     RITLAYEPVWAIGTGRTASPAQAQEVHAAIRQWLAERFDATTAAAARILYGGSVNPGN
                     IASLMCERDVNGALVGGASLECDSFRRIVMFD"
     gene            1696..2073
                     /gene="secG"
                     /locus_tag="PLW16_10205"
     CDS             1696..2073
                     /gene="secG"
                     /locus_tag="PLW16_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB71320.1"
                     /translation="MQVLITILHIIVAIVMILVVLLQAGKGANMGAAFGGSSNTVFGS
                     SGPGTFLGKMTTVVAVIFMLTSLSLSYMVGRTGKTSMMDAVKRPAAERQIPAAPLPPS
                     PVAPQAPGAPAGTPAPAPTPAAR"
     gene            2207..2293
                     /locus_tag="PLW16_10210"
     tRNA            2207..2293
                     /locus_tag="PLW16_10210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2242..2244,aa:Leu,seq:gag)
     gene            complement(2319..2705)
                     /locus_tag="PLW16_10215"
     CDS             complement(2319..2705)
                     /locus_tag="PLW16_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB71321.1"
                     /translation="MEKIPRAIHDENMHKNRVFLFKGKPVKDLRTALTRACRDAGIPY
                     GRGVKNGFVFHDTRHCFNTNMRKSGVPESVIMKITGHSTREMFLWYDTVDASDTKKAV
                     DQMEGFLKSVDQNVDQAPPNRQGVNR"
BASE COUNT          519 a          955 c          904 g          517 t
ORIGIN      
        1 gcacgttcgc ggccgaggtc aaggcggagg ccgacgccct gaccgtcaac ggcaaacgga
       61 tcccgatcac tacccagacg ggcgccctga acgaactgcc ctggaaatcc ctgggggtgg
      121 acatcgtcat ggagtgcacg ggaaaattcc gcaagcgcga cgaactggcc gggcatctgg
      181 ccgccggggc gaggaaggtc ctcctggccg tcccgggcaa gggtctggat gccacgttcg
      241 tgatgggggt taacgaagag acctacgacc cggcgaggca tgacatcgtc tccaacgcct
      301 cgtgcacgac caattgcatg gcccccgtgg ccaaggtgat ccacgacgcg ttcggaatcg
      361 tcaagggact gatgacgacg gtccatgcct acaccatgga tcagcgcctc ctggacggct
      421 cccacaagga tctccggcgg ggacgggcag cggccctctc catcgtcccc acctccaccg
      481 gcgccgcctc ggccgtggcg gaggtcatcc cggagctcga gggaaagctg ggcggcgtgg
      541 ccctgcgggt gcccacgccc aacgtctcgc tcgtcgatct cgccgtcgag gtcggccgga
      601 acgtgacgat cgcggaggtc aacggcgccc tgaaggcggc cgcggagggg cggctcaagg
      661 ggatcatggc cttctgcgaa gaggagctcg tctccatcga cttcacctcg agcccctact
      721 cggccatcgt cgacgcgccg ctcaccggcg tcatcaacgg aaacttcgtg aaggtcttcg
      781 cctggtacga caacgagagc ggctatgcgt gccgcatgcg ggaccttgcc ctgcacatcg
      841 ccgccagatc cttcggagga tgacatgatc cgccccctga tcgcgggcaa ttggaagatg
      901 cacaagaccg tccgggaggc cgtcgccttc gccggccagc tccgggaggc gctgcctgac
      961 ctccaggacc gggaggtcgt cgttgccccg ccctttccgg cgctcgttcc ggtcgccgag
     1021 gcactgcggg gctccgccat cgccctgggg gcgcagaacc tccacgacgc cgcccaaggg
     1081 gcctttaccg gcgaggtgtc ggccgccatg ctggtcgacg ccggctgccg gttcgtcatc
     1141 gtcggccatt cggagcggcg cgccctcttc ggggaaacgg acgcctccat caaccacaaa
     1201 ctcgcccaag cggtgacgaa cggcctcgtc ccgatcttct gcatcggcga aaccctcccg
     1261 gagcgcgagg ccgaccggac cctggccgtc gtcgcccggc agctaaaaga gggattgaat
     1321 aatctttcct ccaatgatat gaagcggatc acgctcgcgt atgagcccgt ctgggccatc
     1381 gggaccggac ggaccgcctc ccccgcccag gcgcaggagg tgcatgccgc catccgccag
     1441 tggctggcgg agcgcttcga cgccacgacg gcggcggcgg cgcgcatcct gtacgggggc
     1501 agcgtgaacc ccggcaacat cgcgagcctc atgtgcgagc gggacgtgaa cggcgcgttg
     1561 gtcggcggcg ccagcctcga gtgcgattcc ttccgcagga tcgtgatgtt cgattaaggc
     1621 cggcgggtcg cgcgggccgg ggccatcgga cgtgcccgag ggcgcgttca ttttgatgaa
     1681 agagagacga aacccttgca agtactgatc acgattctac acatcatcgt cgccattgtc
     1741 atgatcctgg tggtgctcct ccaggcgggc aagggcgcga acatgggcgc ggcgttcggg
     1801 ggatcgagca acaccgtgtt cggcagcagc gggcccggca cgtttctggg caagatgacc
     1861 acggtcgtgg ccgtcatctt catgctgacc tcgctctcgc tgtcctacat ggtcggccgg
     1921 accggcaaga cctccatgat ggacgccgtc aagcggccgg ccgccgaacg tcagatcccg
     1981 gccgcgccgc tgccgccgtc gcccgtcgct ccccaggctc cgggcgcccc agccggaacg
     2041 ccggcaccgg cccccacccc cgccgccagg tagaaaaccg gcgccacggg ccgccggacc
     2101 cgttttgcat tgacaaaccg gacggcaaag tggtagggga tgatccgcat tttcgggggt
     2161 gagcgctctt tccagcaacc ggttcccgga ccggtcagaa cggactgccg aagtggtgga
     2221 atctggtaga cacgctatct tgagggggta gtgggctacg cccgtccggg ttcaagtccc
     2281 ggcttcggca ccataagaaa aacagggggt tggccaggtc atcggttaac cccctgtcga
     2341 tttggggggg cttggtcaac gttttggtca acacttttca ggaacccttc catttggtca
     2401 acagccttct tcgtatctga ggcatcaacc gtgtcatacc acaagaacat ctcccgcgtg
     2461 gagtgaccgg taattttcat aatgaccgat tcaggtacgc ccgacttccg catgttcgtg
     2521 ttgaagcagt gtcgggtatc atggaagaca aacccgttct tcacgcctcg cccgtaggga
     2581 attccggcat ccctacacgc cctggttaat gcggttcgga gatccttgac cggcttgcct
     2641 ttgaacagga agacccggtt cttgtgcatg ttctcgtcat ggattgccct cgggatcttt
     2701 tccaaaacac ccccttgttt tttgagtttt tttatcagaa aagcgctgcg ccccgcgtgg
     2761 acggaaggcc aatatccggc cgccaatgat cgttccgttc aagccgtttg atcgactcat
     2821 tcaaaatccg gccactcgct ctcccgcccg ccatagatcc gagccactcg gaaaatccgt
     2881 aactgtccgg gattg
//
LOCUS       DAOOLJ010000113         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_42076_length_2891_cov_17.680183, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000113 DAOOLJ010000000
VERSION     DAOOLJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42076_length_2891_cov_17.680183"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLW16_10220"
     CDS             complement(<1..345)
                     /locus_tag="PLW16_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB71322.1"
                     /translation="MTEKIKLIDVSKCTGCRACQLACKQWNQMPARQTKNSGTYQNPP
                     DLQWNTWTLIRFQEVVDKTGTLKWLFRKDGCMHCTDSACVKVCPSGALHYTEFGTVGL
                     IKEKCIGCKECIA"
     gene            complement(351..>2891)
                     /gene="fdnG"
                     /locus_tag="PLW16_10225"
     CDS             complement(351..>2891)
                     /gene="fdnG"
                     /locus_tag="PLW16_10225"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HPB71323.1"
                     /translation="TVPALAESFGRGAMTNHWVDFKNSDVILIMGSNPASNHPVAFKW
                     IQEAIDKRGAKLICVDPRFTQSAAKAHLYAPLRSGTDIAFLGGMIKYILDNNLYFEEY
                     VKQYTNASFLVNPAFKLPGDNNGVFSGMKDGKYDKATWTYQAGGGGVISKDPTLKNPN
                     CVFQLFKKQYARYTPEVVSKITGTPQDKLLEVYKLYASTGKPDRAGVELYAMGWTQHT
                     VGVQNIRAMCIIQLLLGNVGIAGGGVAAMRGESNVQGSTDHALLFHIWPGYLNTPRAS
                     LTTLKDYNAKETPSTKEANSLNWWKNKPKYVASFLKSMYGKGVSLDEAYSLMPKIDDG
                     ANHSWLTIFDQMYKGKFTGFFAWGMNPACSGAHSNKVRQALTKLDWMVNVNLFDNETG
                     SFWRGPGMDPKTIKTEVFMLPCASSVEKEGSIANSGRLQQWRYKAVNPPGEALPDGDI
                     MSEIFFKVKALYEKEGGPLKEAVTKLTWPYGTKTVAGHFHYEPRRVAAEINGFFLEDK
                     RVENPTKKGEFKEFKKGDLVPTFAWLQDDGSTSSGCWIYCGSVTGDKGILPMRRGTKD
                     PTGIGLYPEWAWSWPVNRRIIYNGASVDPAGKPWDPKRSVIAWNGEKWVGDVPDGAGN
                     PGSGRPPFIMKPDGVASLFGPGLADGPFPEHYEPLECPVEKNLLSPQKNNPAIKRFDK
                     TGVGSDLDKYSGVDSCDPRFPFICSTYRISEHWQTGVLTRWCPWLAEMQPELWVEISQ
                     ELAKERGFKNGERVVVKSARGEVEAVAIVTPRFKPFDIDGATIHEVGIPWHFGWITSA
                     DRKYADGDKKPEVFTRGDAANLLTPTIGDANTMIPESKAFMVNVVKRG"
BASE COUNT          471 a          880 c          920 g          620 t
ORIGIN      
        1 ggcgatgcac tccttgcagc cgatgcactt ctccttgatc aggcccaccg tgccgaactc
       61 cgtgtagtgc aaggcgcccg aggggcagac cttgacacag gccgaatcgg tgcagtgcat
      121 gcagccgtcc ttccggaaca gccacttgag cgtcccggtc ttgtccacga cctcctggaa
      181 ccggatgagc gtccaggtat tccactgcag gtcgggggga ttctgatacg tcccgctgtt
      241 cttcgtctgc cgggcgggca tctggttcca ctgcttgcag gccagttggc aggcccggca
      301 gccggtgcat ttcgaaacgt cgatgagttt gattttttca gtcatttatc tcaccccctt
      361 ttcacgacgt tgaccatgaa ggctttgctc tcgggaatca tggtgtttgc atcgccgatg
      421 gtcggcgtga ggagattcgc cgcgtcgccg cgggtgaaga cttcgggctt tttgtccccg
      481 tcggcgtact tccggtcggc ggaggtgatc cagccgaagt gccaggggat gcccacttca
      541 tggatggtcg ccccgtcgat atcgaacggc ttgaagcgcg gggtgacgat cgcgaccgcc
      601 tccacctcgc cgcgggcgga tttgacgacg acccgctcgc cgtttttgaa gccccgttcc
      661 ttggccagtt cctggctgat ctccacccag agctccggct gcatttccgc gagccacggg
      721 caccagcggg tgagcacacc cgtctgccag tgttcgctga tccggtaggt gctgcagatg
      781 aaggggaacc gcggatcgca gctgtcgacg ccggaatact tgtccagatc cgatccgaca
      841 ccggtcttgt cgaagcgctt gatggcgggg ttgttcttct gaggagacag gaggttcttc
      901 tccacggggc actccagcgg ttcgtagtgc tccgggaaag gcccgtccgc gagccccggt
      961 ccgaagagac tcgccacgcc gtccggcttc atgatgaagg gcggccggcc cgaacccggg
     1021 ttgccggcac cgtccggaac gtcgccgacc cacttctcgc cgttccaggc gatgacggag
     1081 cgcttgggat cccagggctt gcccgcggga tcgacggagg ccccgttgta gatgatccgg
     1141 cggttgaccg gccaagacca ggcccattca gggtaaaggc ctatgccggt cggatccttc
     1201 gtcccgcggc gcatgggcag aatgcccttg tcgcccgtaa cggacccgca gtaaatccag
     1261 cagccggacg aggtcgatcc gtcgtcctgc agccaggcaa aggtcggcac gagatcgccc
     1321 ttcttgaatt ccttgaactc gcccttcttg gtcgggtttt ccacccgctt gtcttcgagg
     1381 aaaaacccgt tgatctcggc agccaccctc cgcggctcat agtggaagtg gccggcaacg
     1441 gtcttggtgc cgtagggcca agtcagcttg gtgacggcct ccttcagggg gccgccctcc
     1501 ttctcgtaga gcgccttgac cttgaagaag atctcgctca tgatgtcgcc gtcgggcagg
     1561 gcctctcccg ggggattgac ggccttgtag cgccactgct ggagacggcc gctgttggcg
     1621 atgctcccct ccttctcgac ggaggaggcg cagggcagca tgaagacctc ggtcttgatc
     1681 gtcttgggat ccatgccggg gccgcgccaa aaggagccgg tctcgttgtc gaaaaggttg
     1741 acgttgacca tccagtcaag cttcgtgagc gcctggcgga ccttgttgga gtgggcgccg
     1801 ctgcacgccg ggttcatgcc ccaggcgaag aaaccggtga acttgccctt gtacatctgg
     1861 tcgaagatcg tcagccacga gtggttcgcc ccgtcgtcga tcttcggcat caggctgtag
     1921 gcctcgtcga gcgaaacgcc cttgccgtac atggacttga ggaaactggc cacgtacttg
     1981 ggcttgttct tccaccagtt taggctattg gcctccttgg tggacggcgt ttccttggcg
     2041 ttgtaatcct tgagcgtcgt cagggacgcc ctcggtgtgt tcaggtagcc cggccagatg
     2101 tggaaaagca gggcgtggtc cgtggagccc tggacgttcg actcgccgcg catggccgcg
     2161 acgccgccgc cggcaatgcc cacgttgccc agcagaagct ggatgatgca catcgcccgg
     2221 atgttctgaa cgcccaccgt gtgctgcgtc cagcccatcg cgtagagctc gacgccggcc
     2281 cggtccggct tgccggtcga ggcgtacagc ttgtagacct cgagcagttt gtcctggggc
     2341 gtcccggtga tcttcgagac gacctccggc gtgtagcggg cgtactgttt cttgaagagc
     2401 tgaaacacgc agttgggatt cttgagggtc gggtccttgc tgatgacccc gcccccgccg
     2461 gcctggtagg tccaggtcgc cttgtcgtac ttgccgtcct tcatgcccga gaagacgccg
     2521 ttgttgtcgc cggggagctt gaaggccgga ttgaccagga aggaggcgtt ggtgtactgc
     2581 ttgacgtact cctcgaagta caggttgttg tcgaggatgt acttgatcat tccgcccagg
     2641 aaggcgatat cggtcccgga acggaggggg gcgtagagat gggccttggc ggccgactgg
     2701 gtgaaccgcg ggtccacgca gatcagcttc gctcctcttt tgtcgatcgc ctcctggatc
     2761 catttgaagg cgaccggatg gttggaagcg gggttgcttc ccatgatcag gattacatca
     2821 ctgttcttga aatcgaccca gtgattcgtc attgcgccgc gtccgaacga ctctgccaga
     2881 gccggtacag t
//
LOCUS       DAOOLJ010000114         2753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_45094_length_2753_cov_16.720904, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000114 DAOOLJ010000000
VERSION     DAOOLJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45094_length_2753_cov_16.720904"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1475
                     /locus_tag="PLW16_10230"
     CDS             <1..>1475
                     /locus_tag="PLW16_10230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB71324.1"
                     /translation="SQVPDLMRRGMVPIEVALIQTSPPDKHGCMSLGVSVDIVKAATE
                     AAGLVITQVNRHMPRVHGDSFIRIEDVDFIIPHDEPLLEYEAGVDFEAAQRIGKYISE
                     LVQDGDTIQVGYGIIPNALFSNLQHKKHLGVHTELLSDGIVELMKKGVIDNSQKPINR
                     GKTIAAFCMGHRETYAYIDDNPFIEFKTIDYTNNPLIIAQHDNMTAINTGLEIDLTGQ
                     ATAESIGKTFYSGIGGQADFMRGTVLAKNGRTILALLSTADHGRVSRIVPFLKEGVGT
                     SLIRGDIHYVVTEYGIAYLHGKNIRERAMELIAIAHPDFRPALIEEARQGGLIYRDQK
                     FVPGKSGEYPEELETYRTTKTGEEIFLRPVKISDEPLLKEFIYSLSDRSMHRRFITLR
                     KDMHHDRLQEFVAIDYTKKMVIMVFGQDGPKDLLIGVGEYVIVEETHTADVAFMVRDD
                     CQNRGIGTELLHYLTYLAKRQGLLGFTASVISENEPMLHVF"
     assembly_gap    1476..1575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1576..2594)
                     /locus_tag="PLW16_10235"
     CDS             complement(<1576..2594)
                     /locus_tag="PLW16_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPB71325.1"
                     /translation="MFQEYFTEDHQIFRGTVRKFVEKELKPHVEAWEDEELFPIELYK
                     KMADAGLLGIGYPEAYGGVPCDKFMHVAFTEEMIRGGSVGLVSGLGSHSIAMPPVLNL
                     GTEEQKQRFLVPVLAGDKVSALGITEPNAGSDVANLRTRAVRDGDFYVVNGAKTFITS
                     GCRANFITTAVRTGGPGHKGISLLVIDSSTPGFSVSKKIRKMGWAASDTAELSFVDCR
                     VPVENLLGGEGMGFYGIMINFQNERLALSVMAHAVAELALEESIKYAKQREAFGKPLT
                     GFQIIRHKLVDMAMKVDVAKEYNYRLAAKMEAGKDCVREVSMAKNFACSVCDQVVFDA
                     VQIHGG"
BASE COUNT          536 a          843 c          788 g          486 t
ORIGIN      
        1 tcccaggtgc ccgatctgat gcgccggggc atggtcccga tcgaggtggc cctgatccag
       61 acctcgccgc ctgacaaaca cggctgcatg agcctcggcg tcagcgtgga catcgtcaag
      121 gccgccacgg aggcggctgg cctcgtgatc acacaggtca accggcacat gccccgggtc
      181 cacggggaca gcttcatccg gatcgaggac gtcgatttca tcatccccca cgacgagccc
      241 ctcctcgaat acgaagcggg ggtggatttc gaggccgccc agcggatcgg caaatacatc
      301 tccgaactcg tccaggatgg cgatacgatt caggtggggt acgggatcat cccgaacgcc
      361 ctgttttcca acctgcagca caagaaacac ctcggcgtcc atacggaact gctctccgac
      421 ggcatcgtgg agctgatgaa gaagggggtc atcgacaatt cccagaagcc gatcaaccgg
      481 gggaagacca tcgccgcctt ctgcatgggg caccgtgaaa cctatgcata tatcgacgac
      541 aaccccttta tcgagttcaa gaccatcgac tatacgaaca atcccctcat catcgcccag
      601 cacgacaaca tgacggccat caataccggc ctcgagatcg acctgaccgg ccaggccacg
      661 gcggagtcca tcggcaagac cttctacagc gggatcggcg gccaggccga tttcatgcgc
      721 ggcacggtgc tcgcgaaaaa cggacggacg atcctggccc tcctgtccac ggcggaccac
      781 ggccgggttt cgcgaatcgt gccgttcctc aaggaggggg tggggacctc cctgatccgg
      841 ggagacatcc actacgtcgt cacggaatac ggcatcgcct acctgcacgg gaaaaacatc
      901 cgggagcggg ccatggaact catcgccatc gcccaccccg atttccgtcc ggcgctcatc
      961 gaagaggcgc ggcagggcgg cctgatctac cgggaccaga agttcgttcc cggcaagagc
     1021 ggcgaatatc ccgaggaact cgaaacctat cgcacgacga agaccgggga ggagattttc
     1081 ctgaggccgg tcaagatcag cgacgagccc ctgctcaagg agttcatcta ctccctgtcc
     1141 gaccgcagca tgcatcgccg cttcatcacc ctgcgcaagg acatgcacca tgaccggctc
     1201 caggagttcg tcgccatcga ctatacgaag aaaatggtga tcatggtttt cgggcaggac
     1261 ggcccgaagg acctcctcat cggcgtgggg gagtacgtca tcgtggaaga gacccacacg
     1321 gcggacgtgg ccttcatggt ccgcgacgac tgccagaacc ggggcatcgg cacggaactg
     1381 ctgcactacc tgacgtacct cgccaagcgt cagggtctcc tggggttcac ggcctcggtg
     1441 atttcggaga acgagcccat gctccatgtc ttcgannnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnccgcc gtggatctga acggcgtcga agacgacctg atcgcagacc
     1621 gaacaggcga agttcttggc catggagacc tcccggacgc agtccttgcc cgcctccatc
     1681 ttggccgcca gacggtagtt gtattccttg gcgacatcga ctttcatcgc catgtccacg
     1741 agtttgtgcc ggataatctg gaagcccgtc aacggcttcc cgaaggcctc ccgctgtttg
     1801 gcgtacttga tggattcctc cagggccagc tcggccacgg catgggccat gacggacagg
     1861 gcgagccgct cgttctggaa gttgatcatg atgccgtaaa agcccatccc ctcgccgccc
     1921 aggaggtttt ccaccggcac ccgacagtcc acgaaggaca gttccgccgt gtcggaggcg
     1981 gcccagccca tcttccggat ctttttcgaa acgctgaaac cgggcgtgga cgaatcgatc
     2041 acgagcaggc tgatcccctt gtggccgggg ccgccggtcc ggacggcggt cgtgatgaag
     2101 ttggcccggc agccgctggt gatgaaggtc ttggcgccgt tgacgacgta gaaatccccg
     2161 tcgcggacgg cccgcgtccg gagattggcg acatccgagc cggcattggg ttccgtgatg
     2221 ccgagcgcgg agaccttgtc tcccgccagc acgggaacga gaaaacgctg tttctgctcc
     2281 tccgtgccca ggttgagaac cggaggcatg gcgatggagt ggcttcccag gccggagacg
     2341 agaccgacgg aaccgccgcg gatcatctct tcggtgaagg cgacatgcat gaacttgtcg
     2401 caggggacgc cgccgtaggc ctccggatag ccgatcccca gcaggccggc gtcggccatt
     2461 ttcttgtaca attcgatggg aaagagctcc tcgtcctccc aggcctcgac gtggggtttg
     2521 agttccttct ccacgaactt gcggaccgtg ccccgaaaga tctgatggtc ttcggtgaag
     2581 tattcctgaa acattgaaaa cctccttttt ctctatcagg atcgacagtt aagaagaagg
     2641 ggacagcacc tcccggatgc cgcccccttc agacttatgc cttcgcggcg agtttggtct
     2701 cgcattcctt gcgcaggacg ttcttcagga tcttgccgct cgggttgcgc ggc
//
LOCUS       DAOOLJ010000115         2742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_45339_length_2742_cov_20.910681, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000115 DAOOLJ010000000
VERSION     DAOOLJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45339_length_2742_cov_20.910681"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLW16_10240"
     CDS             <1..306
                     /locus_tag="PLW16_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71326.1"
                     /translation="MIGFGILSVAFLAPAAAVIQDVVHPGLRALAYGLCVVCQHVFGA
                     AWSPMLIGNISDHVGLEKALLVLPVFGVVAAVLFMVGARHYEKDLDKVRHVELLSES"
     gene            328..1530
                     /locus_tag="PLW16_10245"
     CDS             328..1530
                     /locus_tag="PLW16_10245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB71327.1"
                     /translation="MFVSHLSCPKCRATYEPNRKIQLCTCGSPLLVAYDLKKTEKALK
                     KDSLPQRRNDLWRYRELLPVRDFKNIVTLGEGMTPLIRLRNIGGEMDLPHLYLKDEGI
                     IPTGTFKARGAAVGVSMAKELGVKALAMPTNGNAGGAWALYCAAAGIQAVIVMPKDAP
                     PIPRKECVIAGASLYLVNGLISDAGRIVGRAVTDRGLYDASTLKEPYRIEGKKTMGLE
                     IAEQFGWDVPEVILYPTGGGVGIIGIYKALQELQEIGWIGSRMPRLVAVQASGCAPVV
                     SAWQAKKTESVFWENSATVAFGINVPKALGDFLVLDAVYATNGCAVAVTDEDILNAQA
                     YLGRREGLFVCPEGAATLAAARRLVREGWIEKNERVVLLNTGTGLKYPDSVDAEPPVL
                     QPGDAIER"
     gene            1550..1936
                     /locus_tag="PLW16_10250"
     CDS             1550..1936
                     /locus_tag="PLW16_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPB71328.1"
                     /translation="MKKIIATENAPKAIGPYSQAVQAGDLLFISGQIPLDPETSEIVG
                     STATDQSEQVFKNIGAILTAAGLGYRDVVKTTVLLKSIQDFAAVNEVYARYFTDAYPA
                     RAAYEVVNLPKGALVEIEAIATLSRT"
     gene            complement(2137..>2742)
                     /locus_tag="PLW16_10255"
     CDS             complement(2137..>2742)
                     /locus_tag="PLW16_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPB71329.1"
                     /translation="QIRAQIEETTSDYDREKLQERLAKLVGGVAVVKVGAATEIEMKE
                     KKARVEDALNATRAAVEEGIIPGGGVAYLRALPALAKERLADDEEQHGINIVKRALEE
                     PLRQIAVNAGFEGSIVVEKVKDGKGNYGFDADRGEYGNLIDAGIMDPTKVARLALLNA
                     ASVAGLLITTEAMVAEKPRKKAPMAMPPGGGMPPDMGDYDY"
BASE COUNT          483 a          896 c          887 g          476 t
ORIGIN      
        1 atgatcggat tcggcatcct gtcggtggcg tttctcgccc ccgcggcggc ggtcattcag
       61 gacgtggtgc acccgggcct ccgcgcgttg gcctacggcc tgtgcgtggt ctgccagcat
      121 gtcttcggcg ccgcgtggtc cccgatgctc atcgggaaca tctccgatca cgtcgggctg
      181 gagaaggcgc tcctcgtgct ccccgtcttc ggggtcgtcg cggccgtgct gttcatggtg
      241 ggcgcccgcc attacgaaaa ggacctcgac aaggtcaggc atgtcgagct gttgagcgaa
      301 tcataattct caagaaggag tggaaacatg tttgtcagcc atctgtcctg cccgaagtgc
      361 cgggccacgt acgaacccaa ccggaagatc cagctctgca cctgcggttc gccgctcctg
      421 gtcgcgtatg atctcaagaa gaccgagaaa gccctgaaaa aagactccct gccccagcgg
      481 agaaacgatc tctggcggta ccgggaactg cttccggtgc gggatttcaa gaatatcgtt
      541 acgctgggcg agggaatgac gccgctcatc aggctgcgga acatcggtgg ggagatggac
      601 ctgccccatc tctacctcaa ggacgaggga atcatcccca cgggaacctt caaggcgcgc
      661 ggggccgccg taggcgtctc catggcaaag gaactcgggg tgaaggcgct ggcgatgccg
      721 accaacggga acgccggcgg cgcttgggcg ctgtactgcg cggcggcagg gatccaggcg
      781 gtgatcgtca tgccgaagga cgccccccca atcccacgca aggaatgcgt tatcgcgggc
      841 gcgtccctgt acctggtgaa cggcctcatc agcgacgcgg gccggatcgt cggccgggcc
      901 gtgacggacc ggggactcta cgacgcctcg acgttgaagg agccgtaccg catcgagggg
      961 aaaaagacca tgggcctcga aatcgccgaa cagttcggct gggatgtgcc cgaggtcatt
     1021 ctctacccga cgggcggcgg ggtcgggatc atcggcatct acaaggcgct ccaagagttg
     1081 caggagatcg gctggatcgg ctcccgcatg ccgcgcctcg tcgcggttca ggcgagcggg
     1141 tgcgccccgg tggtcagcgc ctggcaggct aaaaagaccg aatcggtgtt ctgggaaaac
     1201 tcggccaccg tggccttcgg catcaacgtg ccgaaggcgc tgggcgactt cctggtgctc
     1261 gacgcggtct acgccacgaa cgggtgcgcc gtcgcggtga ccgacgagga tatcctgaac
     1321 gcacaggcgt acctgggtcg gcgggagggg ctgttcgtgt gccccgaggg cgccgcgaca
     1381 ctcgccgccg cccgcaggct ggtccgggag ggctggatcg aaaagaacga acgggtggtg
     1441 ctgctcaata ccggcaccgg cttgaagtat cccgattcgg tggacgcgga gccgccggtc
     1501 ctgcagccgg gcgacgccat cgagcgctga catccaaagg acaaccgaca tgaaaaagat
     1561 cattgcgaca gaaaacgcgc ccaaggccat cggcccgtat tcacaggccg ttcaggcggg
     1621 ggacctgctg ttcatttccg gacagattcc gctcgatccg gaaacatccg agatcgtcgg
     1681 cagcaccgcc accgatcagt ccgagcaggt cttcaagaac atcggggcga ttctcacggc
     1741 cgcggggctc ggctatcggg acgtggtcaa gaccaccgtg ctcctcaaga gcatccagga
     1801 tttcgcggcg gtcaacgagg tgtacgcgcg gtatttcacc gacgcgtatc ccgcccgggc
     1861 ggcgtacgag gtggtcaacc ttccgaaagg cgccctggtc gagatcgagg caatcgccac
     1921 actgtcacgc acgtaagaac ccgccgaacc ggtgcccgga tacgccgtca ggctgctttg
     1981 ggccgggtgc ggattcgccc ccgggcttcc cgcgccacag gtatcgctgc cggccccaac
     2041 gggggaaacg accctccatg aatgaaaaaa gggggcaggc gccttcgcac ctgccccctt
     2101 tttcgtcaca catccggacg gaagcgccgg acggaatcag tagtcataat cccccatgtc
     2161 gggcggcatg ccgccgccgg ggggcatggc catcggggcc ttcttcctgg gcttttcggc
     2221 gaccatcgcc tcggtcgtga tcaacaagcc cgccactgag gccgcgttca ggagcgccag
     2281 gcgggcaacc ttcgtcggat ccatgatccc cgcgtcgatc aggttgccgt actcgccgcg
     2341 gtcggcgtcg aaaccgtagt tccccttgcc gtccttgacc ttctccacaa cgatggaccc
     2401 ctcgaagccc gcgttcacgg cgatctgccg gagcggctcc tccagggcgc gcttcacgat
     2461 gttgatgccg tgctgctcct cgtcgtcggc cagcctttcc tttgccagcg ccggcagggc
     2521 gcgcagatag gccacgccgc ctccgggaat gattccctct tccacggcag cccgcgtggc
     2581 gttcagggcg tcctcgacgc gggccttctt ctccttcatc tcgatctcgg tggccgcccc
     2641 gaccttcacg accgcgaccc cgccgacgag cttggccaga cgctcctgga gcttctcccg
     2701 gtcgtagtcg gaggtggtct cctcgatctg ggcgcggatc tg
//
LOCUS       DAOOLJ010000116         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_46637_length_2685_cov_19.123194, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000116 DAOOLJ010000000
VERSION     DAOOLJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46637_length_2685_cov_19.123194"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLW16_10260"
     CDS             <1..837
                     /locus_tag="PLW16_10260"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HPB71330.1"
                     /translation="PLLERGRIRGYAYPALEAGEDGCRPRLCTEPGEAEGVHRVGVMR
                     LACLCLPGELKSLKKAVALPAAMKAQAAFCGGPAAWERRILEKVLGDLVDVRVRGRAD
                     FQNMIAGLRARLLPHAQDVTALVTPVLRATSDASQTLNALTAAHRFNRPALAFLAALK
                     EELARLIPDDFLQVYSVDRLVHVPRYLAALSIRAQRGLDHLDRALAREEPLRAYAKRL
                     AEFTAEITPQTTAEKRREIEALFWMIEEYKVSVFAQELKTPAPVSPKRLQAKIAAIEA
                     LP"
     gene            complement(845..1528)
                     /locus_tag="PLW16_10265"
     CDS             complement(845..1528)
                     /locus_tag="PLW16_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB71331.1"
                     /translation="MTKPEWIAEGVCQVGGPEISRADDATVFIVDGGDELAMIDSGAG
                     RSVRRIEDNIRNAGLDPSRITQLILTHCHIDHIGGAPHFRERFGCRILIHTLDAEAVE
                     AGDPKRTAANWYDTDFPPTRMDGRLSGDAGALAIGNTALSWVHTPGHTPGSICLYLDR
                     ASERILFGQDIHGPFLAAFDSDLAQWRRSMDKLLALDADILCEGHFGIFRTKPSVQRY
                     IQRTLDQNR"
     gene            complement(1525..2493)
                     /locus_tag="PLW16_10270"
     CDS             complement(1525..2493)
                     /locus_tag="PLW16_10270"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPB71332.1"
                     /translation="MKPSTSIDLKVSIGDLVLNNPVMTASGTFGYGEEFAPYGDLNHL
                     GAVIVKGLSLEPRTGNPPPRMMETPSGMLNAVGLQNIGVRAFIREKLPFLRRFDCAVI
                     ANIYGETVEEYERVAGLLSRASGVAALEVNISCPNVRRGGIAFGAEPDAAAEVTRRVK
                     AAAALPVMVKLTPNVTDIAQIARAVEEAGADAISLINTLTGMSVDVEKRIPHLANVTG
                     GLSGPAIRPVALRMVWQAVQAVSIPVIGVGGILNARDALEFLIVGARAVQVGTANFIR
                     PTVTMEIIDGISAYLERHACRSVTQIIGTLKTPEAELRRGGGGERT"
     gene            complement(2490..>2685)
                     /locus_tag="PLW16_10275"
     CDS             complement(2490..>2685)
                     /locus_tag="PLW16_10275"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB71333.1"
                     /translation="CQVSVEERMACGIGACLCCATAVAMPGRQVSYRRVCCDGPVFNL
                     RDLIWDMTPDHSDSRGCTSP"
BASE COUNT          455 a          876 c          879 g          475 t
ORIGIN      
        1 cccctgctcg aacgcggccg catccggggg tatgcctacc cggccctcga ggccggcgag
       61 gacggctgcc gaccccggct ttgcacggaa ccgggcgagg cggaaggcgt gcatcgcgtg
      121 ggcgtcatgc gtctggcgtg cctctgcctc ccgggtgaat tgaagtcgct gaagaaggcc
      181 gtcgcactcc ccgcggccat gaaggcgcaa gccgcttttt gtgggggccc ggcggcgtgg
      241 gaacggcgga tcctcgaaaa ggtgctgggc gatctcgtcg atgtccgcgt ccgcggacgg
      301 gcggatttcc agaacatgat cgctggtctg cgcgcccggc tactgcctca cgcgcaggac
      361 gtgaccgccc tcgtcacgcc cgtcctgagg gccacctccg acgcctcgca gacgctgaac
      421 gctcttacgg cggcgcaccg cttcaaccgg ccggccctcg cctttctggc ggcgctcaag
      481 gaggaactgg cccgtctgat cccggacgac ttcctccaag tctattccgt cgaccgcctc
      541 gtccacgtcc cccgctatct cgccgccctg tcgatccgcg cccaacgcgg gctcgatcat
      601 ctggaccgtg cgctcgcccg ggaagagccc ctgcgcgcct atgcgaagcg cctggcggaa
      661 ttcacggcgg agatcacgcc ccagaccacg gcggaaaagc gtcgggaaat cgaggccctc
      721 ttttggatga tcgaggaata caaggtctcc gtctttgccc aggagctgaa gacccccgcg
      781 cccgtctctc ccaagcgttt gcaggctaaa atcgcggcga tcgaggccct tccctgaggg
      841 aggatcaccg gttctggtcg agggtgcgct gaatgtagcg ctggacgctt ggctttgtcc
      901 tgaagattcc gaaatggccc tcgcagagga tgtcggcgtc aagcgcgagg agtttatcca
      961 tggaccggcg ccactgagcg agatcggagt cgaaggcggc cagaaaaggg ccgtggatat
     1021 cctgaccgaa caggatccgc tcgctcgccc ggtcgaggta aaggcagatc gaccccgggg
     1081 tgtgccctgg cgtatggacc caggacagcg cggtgtttcc gatggccaga gcgcccgcgt
     1141 ctccgctcag ccgtccgtcc attctggttg gaggaaaatc cgtgtcatac cagttcgcgg
     1201 cggtgcgttt cgggtcgccc gcctccacgg cctcggcgtc gagggtgtgg ataaggatcc
     1261 ggcagccaaa gcgttcgcga aagtggggcg cccccccgat gtgatcgata tggcagtgtg
     1321 tcagaatcag ctgtgtgatc cgggagggat cgagtcccgc gttccggatg ttgtcttcaa
     1381 tcctccggac gctgcgcccg gcccctgaat cgatcatggc gagttcatcc ccaccgtcta
     1441 cgataaaaac ggtcgcgtca tcagcccggg agatctccgg acctcccacc tggcaaaccc
     1501 cctcggcaat ccattccggc tttgtcatgt ccgttcgcct ccccctcccc ttcgtaattc
     1561 ggcttccggc gtcttcaggg tgccgatgat ctgcgtcacg gaccggcatg cgtgccgctc
     1621 caggtaggcg gatatcccgt cgatgatctc catcgtcacg gtgggacgga tgaaattggc
     1681 cgtgcccacc tggacggccc tggcgccgac gatcaggaat tccaaagcat cccgggcgtt
     1741 caggatgccg ccaacgccga tcacggggat agaaacggcc tgcaccgcct gccagaccat
     1801 cctcagggcg accggtcgaa tggcgggacc ggagaggccg ccggtgacgt ttgccagatg
     1861 ggggatccgc ttctcgacat cgaccgacat cccggtcagg gtgttgatca gggagatggc
     1921 gtctgcgccg gcctcctcga ccgcccgggc gatttgggca atgtccgtga cattgggcgt
     1981 gagtttgacc atgacgggga gcgccgccgc cgccttgacg cgccgggtga cctccgccgc
     2041 ggcgtcgggc tcggcgccga aagcaatgcc gccgcgccga acattcgggc aggagatgtt
     2101 cacctcgagc gcggctaccc cggaggcccg gctcagcagg cctgcgaccc gttcgtactc
     2161 ctcgacggtc tcaccgtaga tgttcgcgat gaccgcacag tcgaaccgtc tgaggaaagg
     2221 cagtttctcc cgaatgaagg cacgcacgcc gatgttctgc agtccgacgg cgttgagcat
     2281 gccggacggc gtttccatca tgcggggcgg gggattgccg gttctgggct cgagggagag
     2341 ccctttgacg atcaccgctc ccagatggtt caggtcgccg tagggggcga attcctcgcc
     2401 gtaaccgaag gtgcccgagg ccgtcatgac gggattgttg agaacgaggt ccccgatgct
     2461 gaccttcagg tcgatggacg tggacggttt catggcgatg tgcaccccct ggaatcggag
     2521 tggtcgggcg tcatgtccca gatgaggtcc cgcaggttga agacgggccc atcgcagcag
     2581 acgcgacggt aggaaacctg acggccgggc attgcaacag ccgtggcaca gcacaggcag
     2641 gcgccgatgc cgcaggccat ccgctcctcg acggagacct ggcag
//
LOCUS       DAOOLJ010000117         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_49714_length_2566_cov_14.704898, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000117 DAOOLJ010000000
VERSION     DAOOLJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49714_length_2566_cov_14.704898"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1570
                     /locus_tag="PLW16_10280"
     CDS             245..1570
                     /locus_tag="PLW16_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB71334.1"
                     /translation="MQTVDILVTSGTVLTFDDKDTKLIDGALAVHGDRIVALGRRDEL
                     ARTFEARETIDATGAIVMPGLINAHTHAAMTCFRGIADDMELMDWLNNYIFPAEARNV
                     NPELAYWGSLLACAEMIKSGTTTFADMYIFQGETARAAKEAGMRCLIGEVLFDFPSPN
                     INTPAEGLAYTEKLIQTWADDPLVNVMVEPHALYTCSPDLLKDCKALANRYQVPLATH
                     LLENAGEVKQLRDKLGRRATAFLEDIGLLDERFIGFHCVMMDEQDMRLFADHGCKVVH
                     NPESNMKLASGVAPISAMLGHGIAVGLGTDGCASNNNLDMFQELDTAAKLEKSARLDP
                     TVMSARTVLRMATSDGAKVLGLDHLVGTLAVGKKADLCIIDMNKPHLTPLYDEYSHLT
                     YAVSGADVDTVLINGRVVLRSRRLTTIDEIEVMRRVREIAVRIRESLRR"
     gene            1574..>2566
                     /locus_tag="PLW16_10285"
     CDS             1574..>2566
                     /locus_tag="PLW16_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB71335.1"
                     /translation="MPALDLLIRGGTALTLTDDRPRIQPAMIGIRDGLITLLEEGFAT
                     ESRSDLSAAETLDASGTIILPGLVNTHTHLPMTLFRGLADDLPLMDWLNHHIFPAEAR
                     HIHPDTVYAGSLLALAEMILSGTTTFCDGYFYEEHVAQAALDTRMRGIVAEGFLDPPL
                     PEAPDTAAQVRRAERFLAKWQGASPLVTPALFLHSPYTCSPATLTAIKDVARHHGALC
                     CIHVAETREETAIVRDRYGTTPVRHLHRLGVLDEKTLAVHVNWVDEEEMTILLDCGVA
                     VSHCPESSMKLSAGIAPVPALLNRGIPVGLGTDGCASNNDLDLFGEMDTAAKLHK"
BASE COUNT          501 a          860 c          742 g          463 t
ORIGIN      
        1 aggaccgagg gattttaagt cccttgcgtc taccaattcc gccacccagg ctttgtcgtg
       61 cgcgcctcac gctatagtat gtttgttttg gggtgtcaac aagatcgtcc tccggcgccg
      121 cggccctccc ttgagaccgg ttttaaagtc atttccgaaa ggttcgcccg tcttgaatta
      181 ggttgaagag cctttcttca atgtgctaat ggcagatcaa tacccaatgc aagaggatgc
      241 gaagatgcaa acagtcgata tcctggtaac cagcggaacc gtcctgacgt tcgacgataa
      301 ggacacgaaa ctgatcgacg gggcgctggc cgtccacggc gaccgcattg tcgcgctggg
      361 tcgccgggac gaattggccc gaacctttga ggccagggag acgatcgacg cgaccggcgc
      421 catcgttatg ccggggctca tcaacgccca cacgcacgcc gccatgactt gcttccgcgg
      481 catcgccgac gacatggaac tcatggactg gctgaacaat tacatcttcc cggccgaggc
      541 ccgcaacgtg aaccccgaac tggcctactg gggcagcctg ctcgcctgcg ccgagatgat
      601 caagtcgggg acgaccacct tcgcggacat gtacatcttc caaggggaaa cggcccgcgc
      661 ggccaaagag gcggggatgc ggtgcctgat cggcgaggtc ctcttcgact tcccctctcc
      721 caacatcaac acccccgccg aaggcctcgc ctatacggaa aaactcatcc aaacctgggc
      781 cgacgacccg ctcgtcaacg tcatggtcga accgcatgcg ctgtacacgt gctcaccgga
      841 tcttttgaag gactgcaagg ccctggcgaa ccgctaccag gtccccctcg cgacgcacct
      901 gctggaaaac gcgggcgagg tcaaacagct acgggacaag ctgggccgcc gggcgacggc
      961 ctttcttgag gacatcggcc tgctcgatga acggtttatc ggctttcact gcgtcatgat
     1021 ggacgaacag gacatgcgcc tcttcgccga tcacggctgc aaggtggtgc acaaccccga
     1081 gagcaacatg aagctggcct ccggcgtcgc gccgatctcc gccatgctcg ggcacgggat
     1141 cgccgtcggt cttggcacgg acggttgcgc gagcaacaac aacctggaca tgttccagga
     1201 gctggacacc gcggccaaac tggaaaagtc cgcccggctc gatcccacgg tcatgtcggc
     1261 ccggaccgtc ctcaggatgg cgacgtccga cggggcgaag gtcctcggct tggatcatct
     1321 cgtcggcaca ctcgcggtcg gcaaaaaggc cgatctctgt atcatcgata tgaacaagcc
     1381 ccatttgacg cccctgtacg acgaatactc gcacctgacc tatgccgtct ccggcgccga
     1441 cgtggacacg gttctcatca acggccgggt ggtcctgcgg agccggagac tgaccacgat
     1501 cgacgagatc gaggtcatgc ggcgggtgcg cgagatcgcc gtgcgcatcc gcgagagcct
     1561 ccggaggtaa ggcatgcccg cgctcgatct cctcatccgg ggcggaacgg ccctgaccct
     1621 gaccgacgac cggccacgga ttcagccggc catgatcggc atccgggacg gcctcatcac
     1681 cctgcttgaa gaagggttcg ccaccgaatc ccgaagcgac ctgagcgctg ccgaaaccct
     1741 tgacgcctcc gggacgatca ttctgccggg tcttgtcaat acgcacaccc atctccccat
     1801 gacccttttc cgcggtctcg ccgatgacct gcctctgatg gactggctga accaccatat
     1861 tttccccgct gaggcgcgcc atattcatcc ggataccgtc tatgccgggt cgcttctcgc
     1921 tctggccgag atgatcctgt ccggaacgac cacgttctgc gacggctatt tttacgagga
     1981 acacgtcgcc caggcggcgc tcgacaccag gatgcgggga atcgtggccg agggattcct
     2041 cgaccctccc ctgccggagg cgcccgatac cgcggcccag gtgcgccggg cggagcgctt
     2101 cctcgccaag tggcagggcg cctccccgct cgtcaccccc gccctcttcc ttcattcacc
     2161 gtacacctgc tcgccagcca ccctaacggc catcaaggac gtcgcccgtc atcacggtgc
     2221 actctgctgc attcacgtgg cggaaacgag ggaggagacc gccatcgtcc gggaccggta
     2281 cggaacgacg cccgtccgcc acctccaccg tctgggcgtg cttgacgaga agaccctggc
     2341 ggttcatgtc aactgggtgg acgaggaaga gatgacgatc ctccttgatt gcggcgtggc
     2401 cgtctcccac tgcccggaga gcagcatgaa gctctccgcc ggcatcgcac ccgttccggc
     2461 tctgctcaat cgcggcattc cagtcggact tggaacggat ggttgtgcca gcaacaacga
     2521 cctcgatctg ttcggagaaa tggacaccgc cgcgaagctt cacaag
//
LOCUS       DAOOLJ010000118         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2704
            NODE_68243_length_2062_cov_11.419033, whole genome shotgun
            sequence.
ACCESSION   DAOOLJ010000118 DAOOLJ010000000
VERSION     DAOOLJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864779
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,060
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 2,024
            CDSs (with protein)               :: 2,024
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68243_length_2062_cov_11.419033"
                     /isolate="AMR_MDS_2704"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLW16_10290"
     CDS             complement(<1..205)
                     /locus_tag="PLW16_10290"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB71336.1"
                     /translation="MKILALQGSPRPAGYTQMVLDPFLEGARSRGAETETIFLAKQKI
                     RPCIGCYTCWYKTPGICIHKNDDM"
     gene            518..1087
                     /locus_tag="PLW16_10295"
     CDS             518..1087
                     /locus_tag="PLW16_10295"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPB71337.1"
                     /translation="MTGIILSGGKNTRMGRNKALLTLDGERLIDRTVNLFKDLFAEVI
                     IVTNAPREYLDQDVAIVTDVYKGKGALGGIYTGLFYATHDYVFVAACDMPFLRKDFIR
                     HMMEQAPGNDIVVPFQPEGYQPLHAIYSKRCAAPMKRLMDENRLKVTGFYTGMKIHTL
                     SEEEVRSFDPAGWMFLNINSPADLKKIAS"
     gene            1381..>2062
                     /locus_tag="PLW16_10300"
     CDS             1381..>2062
                     /locus_tag="PLW16_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPB71338.1"
                     /translation="MQYETIQYETAEPGIGVLTLNRPRKYNAVNQAMMKELEHFWAER
                     ENDPDTRIVLLKGNGEKGFCAGLDMEEGLELQKTMNSLQFYQSQARMGRLMLKMRQVP
                     QIVVCIVHGAAAGIGLSFALASDLRVISPDARFSAAYINIGLGGADMACSYLLPRAIG
                     AGRAYEFMLTGNYMSAAEAVSLGLVSRMVERDRLTDTALELARTMNGKNPAGLRLTKE
                     AINVNLDIG"
BASE COUNT          411 a          659 c          609 g          383 t
ORIGIN      
        1 gcatgtcgtc gtttttatgg atgcagattc cgggggtctt gtaccagcag gtgtaacagc
       61 cgatgcaggg acggatcttc tgcttggcca ggaagatcgt ttccgtttcg gcgccgcggc
      121 tccgggcgcc ttccaggaac gggtccaaca ccatttgggt atagcccgcc gggcgggggc
      181 tcccctggag cgccagaatc ttcatccgtt cgtctccttt cgattgcggg gatgcgcccg
      241 ttgcgaagcg tccagcgcac ggggacatcg ccgttttcag ggatgtcact atgcacaatc
      301 aggggctttt ttccattaaa atatcccgcc gtcattttca ttgacacaac ccggtgggtt
      361 gcctatgctt cccgccgtga gaggcgatcc tcctccggcg gcccggcatt tttcgcttta
      421 tctttccgcg gagatcggtt agctttcccg caccgggccg accggcgccc cacgggagtc
      481 gaagcgggcg cggacagctc cgaagaagga gcagcacgtg acgggcatca tcctttccgg
      541 cggcaaaaat acgcgtatgg gccgcaacaa ggccctgctc accctcgacg gcgaacggct
      601 catcgaccgg acggtaaacc tgttcaagga cctcttcgcc gaggtcatca tcgtcaccaa
      661 cgccccgcga gaatacctcg accaggacgt cgccatcgtc acggacgtct acaagggcaa
      721 gggggcgctg ggcgggatct acacggggct cttctatgcc acccacgact acgtcttcgt
      781 cgccgcctgc gacatgccct tcctccggaa agacttcatc cggcacatga tggaacaggc
      841 cccgggcaac gatatcgtcg tccccttcca gcccgaaggc taccagccgc tccacgccat
      901 ttacagtaag cgttgcgccg cccccatgaa gcgcctgatg gacgaaaacc gcctgaaggt
      961 caccggattc tacaccggga tgaagatcca taccctatcg gaagaggagg tccgctcctt
     1021 cgatcccgcc ggctggatgt tcctgaacat caattcgcca gcggatctga aaaagatcgc
     1081 ctcgtgacgt gttcgtccgg gcgttgcctg cgcccgtcga ccggcgcgtg cgtcgacgcg
     1141 ttcacccttc gccaaaccca ggctgacaaa cggggacccc gatcaaggcc tccgtctgat
     1201 tgcccgaaga tcgaaataac gagaccttgc atcatgtcat ttcgaggagc acgagtgacg
     1261 agacatcttc acgatggccg tttcttaaag atttctcccc tcggtcgaaa tgacaagaag
     1321 gacggtcgaa gggacatgat gtgaaggttt cataaccttg aaaaggaggg agatttttgc
     1381 atgcaatacg agacgattca gtatgaaacg gcggagcccg gcatcggcgt tctgaccctc
     1441 aaccggccgc ggaagtacaa cgcggtcaac caggccatga tgaaggagct ggaacacttc
     1501 tgggccgagc gcgagaacga ccctgacacg cgcatcgtcc tgctcaaggg caacggcgag
     1561 aagggcttct gcgccgggct cgacatggag gagggcctgg agcttcagaa gaccatgaac
     1621 tccctccagt tctaccagtc ccaggcccgc atgggcaggc tgatgctgaa gatgcgccag
     1681 gttccccaga tcgtcgtctg catcgtgcac ggggcggcgg cgggcatcgg cctgagcttc
     1741 gccctcgcgt cggacctgcg cgtcatcagc cccgacgccc gtttctcggc cgcctacatc
     1801 aacatcggcc tggggggcgc cgacatggcg tgcagctatc tcctgccccg cgccatcgga
     1861 gccgggcggg cgtacgagtt catgctgacc gggaactaca tgtccgccgc ggaggccgtg
     1921 agcctcggct tggtcagccg catggtcgag cgggaccgcc tgacggatac cgcgctcgaa
     1981 ctcgcccgca ccatgaacgg caagaacccg gccggactgc gcctgaccaa ggaggccatc
     2041 aacgtcaacc tggacatcgg cg
//
